LOCUS       DACDIP010000001       115928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDIP010000001 DACDIP010000000
VERSION     DACDIP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 115928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.001"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(606..962)
                     /locus_tag="IV257_000001"
     CDS             complement(606..962)
                     /locus_tag="IV257_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8813164.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IV257_000002"
     CDS             complement(976..1386)
                     /locus_tag="IV257_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8813165.1"
                     /translation="MIKVYTSPSCTSCRKAKAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="IV257_000003"
     CDS             complement(1732..3918)
                     /locus_tag="IV257_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8813166.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="IV257_000004"
     CDS             4136..4819
                     /locus_tag="IV257_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8813167.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="IV257_000005"
     CDS             complement(4900..5619)
                     /locus_tag="IV257_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8813168.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="IV257_000006"
     CDS             complement(5931..6137)
                     /locus_tag="IV257_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813169.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="IV257_000007"
     CDS             6382..6984
                     /locus_tag="IV257_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8813170.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8405
                     /locus_tag="IV257_000008"
     CDS             7125..8405
                     /locus_tag="IV257_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8813171.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8418..9146
                     /locus_tag="IV257_000009"
     CDS             8418..9146
                     /locus_tag="IV257_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8813172.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9215..10738)
                     /locus_tag="IV257_000010"
     CDS             complement(9215..10738)
                     /locus_tag="IV257_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8813173.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10990..12171
                     /locus_tag="IV257_000011"
     CDS             10990..12171
                     /locus_tag="IV257_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8813174.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12231..13859)
                     /gene="treC"
                     /locus_tag="IV257_000012"
     CDS             complement(12231..13859)
                     /gene="treC"
                     /locus_tag="IV257_000012"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8813175.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14310..17021)
                     /locus_tag="IV257_000013"
     CDS             complement(14310..17021)
                     /locus_tag="IV257_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8813176.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17031..17732)
                     /locus_tag="IV257_000014"
     CDS             complement(17031..17732)
                     /locus_tag="IV257_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813177.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17918..18517
                     /locus_tag="IV257_000015"
     CDS             17918..18517
                     /locus_tag="IV257_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8813178.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18598..19611
                     /locus_tag="IV257_000016"
     CDS             18598..19611
                     /locus_tag="IV257_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8813179.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19719..20636
                     /gene="rbsK"
                     /locus_tag="IV257_000017"
     CDS             19719..20636
                     /gene="rbsK"
                     /locus_tag="IV257_000017"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8813180.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20664..21059
                     /gene="rbsD"
                     /locus_tag="IV257_000018"
     CDS             20664..21059
                     /gene="rbsD"
                     /locus_tag="IV257_000018"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8813181.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21059..22432
                     /locus_tag="IV257_000019"
     CDS             21059..22432
                     /locus_tag="IV257_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8813182.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22789..24933
                     /locus_tag="IV257_000020"
     CDS             22789..24933
                     /locus_tag="IV257_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8813183.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFNHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24985..26025)
                     /locus_tag="IV257_000021"
     CDS             complement(24985..26025)
                     /locus_tag="IV257_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8813184.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26302..28104)
                     /locus_tag="IV257_000022"
     CDS             complement(26302..28104)
                     /locus_tag="IV257_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813185.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28118..28870)
                     /locus_tag="IV257_000023"
     CDS             complement(28118..28870)
                     /locus_tag="IV257_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813186.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28879..29241)
                     /locus_tag="IV257_000024"
     CDS             complement(28879..29241)
                     /locus_tag="IV257_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8813187.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29417..30289)
                     /locus_tag="IV257_000025"
     CDS             complement(29417..30289)
                     /locus_tag="IV257_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8813188.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30340..30828)
                     /locus_tag="IV257_000026"
     CDS             complement(30340..30828)
                     /locus_tag="IV257_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8813189.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30966..31796)
                     /locus_tag="IV257_000027"
     CDS             complement(30966..31796)
                     /locus_tag="IV257_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8813190.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(31812..32420)
                     /locus_tag="IV257_000028"
     CDS             complement(31812..32420)
                     /locus_tag="IV257_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8813191.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32413..32730)
                     /locus_tag="IV257_000029"
     CDS             complement(32413..32730)
                     /locus_tag="IV257_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8813192.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32886..33614)
                     /locus_tag="IV257_000030"
     CDS             complement(32886..33614)
                     /locus_tag="IV257_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8813193.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33773..34654
                     /locus_tag="IV257_000031"
     CDS             33773..34654
                     /locus_tag="IV257_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8813194.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34891..35952)
                     /locus_tag="IV257_000032"
     CDS             complement(34891..35952)
                     /locus_tag="IV257_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8813195.1"
                     /translation="MRRLFKDEKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRH
                     SISPAESAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFS
                     IRMDEQQKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLL
                     QAPNLSDEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREV
                     IVFFQPKWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFE
                     TATEIVLEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRI
                     DYSSIEGVGTTAMIRLSKQ"
     gene            complement(35936..36604)
                     /locus_tag="IV257_000033"
     CDS             complement(35936..36604)
                     /locus_tag="IV257_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8813196.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36746..39082
                     /locus_tag="IV257_000034"
     CDS             36746..39082
                     /locus_tag="IV257_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8813197.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39197..39508)
                     /locus_tag="IV257_000035"
     CDS             complement(39197..39508)
                     /locus_tag="IV257_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813198.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39689..41164
                     /locus_tag="IV257_000036"
     CDS             39689..41164
                     /locus_tag="IV257_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8813199.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41211..41795
                     /locus_tag="IV257_000037"
     CDS             41211..41795
                     /locus_tag="IV257_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8813200.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41797..42216
                     /locus_tag="IV257_000038"
     CDS             41797..42216
                     /locus_tag="IV257_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8813201.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42497..42781)
                     /locus_tag="IV257_000039"
     CDS             complement(42497..42781)
                     /locus_tag="IV257_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8813202.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43133..43843)
                     /locus_tag="IV257_000040"
     CDS             complement(43133..43843)
                     /locus_tag="IV257_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8813203.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43840..45312)
                     /locus_tag="IV257_000041"
     CDS             complement(43840..45312)
                     /locus_tag="IV257_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8813204.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45450..45923
                     /locus_tag="IV257_000042"
     CDS             45450..45923
                     /locus_tag="IV257_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8813205.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(46076..46627)
                     /locus_tag="IV257_000043"
     CDS             complement(46076..46627)
                     /locus_tag="IV257_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8813206.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46732..47262
                     /locus_tag="IV257_000044"
     CDS             46732..47262
                     /locus_tag="IV257_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8813207.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47745..48188)
                     /locus_tag="IV257_000045"
     CDS             complement(47745..48188)
                     /locus_tag="IV257_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8813208.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48204..49364)
                     /locus_tag="IV257_000046"
     CDS             complement(48204..49364)
                     /locus_tag="IV257_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8813209.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49871..51571
                     /locus_tag="IV257_000047"
     CDS             49871..51571
                     /locus_tag="IV257_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813210.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51673..52506)
                     /locus_tag="IV257_000048"
     CDS             complement(51673..52506)
                     /locus_tag="IV257_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8813211.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52810..54975)
                     /locus_tag="IV257_000049"
     CDS             complement(52810..54975)
                     /locus_tag="IV257_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8813212.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTCFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55235..57529
                     /locus_tag="IV257_000050"
     CDS             55235..57529
                     /locus_tag="IV257_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8813213.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57522..58187
                     /gene="pgmB"
                     /locus_tag="IV257_000051"
     CDS             57522..58187
                     /gene="pgmB"
                     /locus_tag="IV257_000051"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8813214.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58180..59187
                     /locus_tag="IV257_000052"
     CDS             58180..59187
                     /locus_tag="IV257_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8813215.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59209..60228
                     /locus_tag="IV257_000053"
     CDS             59209..60228
                     /locus_tag="IV257_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8813216.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60509..61876)
                     /gene="nhaC"
                     /locus_tag="IV257_000054"
     CDS             complement(60509..61876)
                     /gene="nhaC"
                     /locus_tag="IV257_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8813217.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61985..63406)
                     /locus_tag="IV257_000055"
     CDS             complement(61985..63406)
                     /locus_tag="IV257_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8813218.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63649..65526)
                     /gene="tdc"
                     /locus_tag="IV257_000056"
     CDS             complement(63649..65526)
                     /gene="tdc"
                     /locus_tag="IV257_000056"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8813219.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRNVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65817..67073)
                     /locus_tag="IV257_000057"
     CDS             complement(65817..67073)
                     /locus_tag="IV257_000057"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8813220.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            67653..68954
                     /locus_tag="IV257_000058"
     CDS             67653..68954
                     /locus_tag="IV257_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8813221.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(69023..69622)
                     /locus_tag="IV257_000059"
     CDS             complement(69023..69622)
                     /locus_tag="IV257_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8813222.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            69876..70757
                     /locus_tag="IV257_000060"
     CDS             69876..70757
                     /locus_tag="IV257_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813223.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            70770..72380
                     /locus_tag="IV257_000061"
     CDS             70770..72380
                     /locus_tag="IV257_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8813224.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(72457..73116)
                     /locus_tag="IV257_000062"
     CDS             complement(72457..73116)
                     /locus_tag="IV257_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8813225.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(73399..74064)
                     /locus_tag="IV257_000063"
     CDS             complement(73399..74064)
                     /locus_tag="IV257_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813226.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(74211..74816)
                     /gene="dhaL"
                     /locus_tag="IV257_000064"
     CDS             complement(74211..74816)
                     /gene="dhaL"
                     /locus_tag="IV257_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8813227.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(74844..75827)
                     /gene="dhaK"
                     /locus_tag="IV257_000065"
     CDS             complement(74844..75827)
                     /gene="dhaK"
                     /locus_tag="IV257_000065"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8813228.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(75829..76224)
                     /gene="dhaM"
                     /locus_tag="IV257_000066"
     CDS             complement(75829..76224)
                     /gene="dhaM"
                     /locus_tag="IV257_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8813229.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            76378..76698
                     /locus_tag="IV257_000067"
     CDS             76378..76698
                     /locus_tag="IV257_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8813230.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(76810..77679)
                     /locus_tag="IV257_000068"
     CDS             complement(76810..77679)
                     /locus_tag="IV257_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8813231.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(77775..79079)
                     /locus_tag="IV257_000069"
     CDS             complement(77775..79079)
                     /locus_tag="IV257_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8813232.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIANAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(79076..79585)
                     /locus_tag="IV257_000070"
     CDS             complement(79076..79585)
                     /locus_tag="IV257_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813233.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(79760..81895)
                     /locus_tag="IV257_000071"
     CDS             complement(79760..81895)
                     /locus_tag="IV257_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8813234.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(81978..83633)
                     /locus_tag="IV257_000072"
     CDS             complement(81978..83633)
                     /locus_tag="IV257_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8813235.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(83635..83949)
                     /locus_tag="IV257_000073"
     CDS             complement(83635..83949)
                     /locus_tag="IV257_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8813236.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            84119..84379
                     /locus_tag="IV257_000074"
     CDS             84119..84379
                     /locus_tag="IV257_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8813237.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            84393..84707
                     /locus_tag="IV257_000075"
     CDS             84393..84707
                     /locus_tag="IV257_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8813238.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(85437..85991)
                     /locus_tag="IV257_000076"
     CDS             complement(85437..85991)
                     /locus_tag="IV257_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8813239.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(86117..88046)
                     /locus_tag="IV257_000077"
                     /pseudo
     CDS             complement(86117..88046)
                     /locus_tag="IV257_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(88352..89068)
                     /locus_tag="IV257_000078"
     CDS             complement(88352..89068)
                     /locus_tag="IV257_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8813240.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(89069..90892)
                     /gene="glpO"
                     /locus_tag="IV257_000079"
     CDS             complement(89069..90892)
                     /gene="glpO"
                     /locus_tag="IV257_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8813241.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(90906..92402)
                     /gene="glpK"
                     /locus_tag="IV257_000080"
     CDS             complement(90906..92402)
                     /gene="glpK"
                     /locus_tag="IV257_000080"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8813242.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(92538..93998)
                     /locus_tag="IV257_000081"
     CDS             complement(92538..93998)
                     /locus_tag="IV257_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8813243.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(93988..95283)
                     /locus_tag="IV257_000082"
     CDS             complement(93988..95283)
                     /locus_tag="IV257_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8813244.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(95615..96292)
                     /locus_tag="IV257_000083"
     CDS             complement(95615..96292)
                     /locus_tag="IV257_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8813245.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(96322..97413)
                     /locus_tag="IV257_000084"
     CDS             complement(96322..97413)
                     /locus_tag="IV257_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8813246.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(97514..97948)
                     /locus_tag="IV257_000085"
     CDS             complement(97514..97948)
                     /locus_tag="IV257_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8813247.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(98532..99989)
                     /gene="ascB"
                     /locus_tag="IV257_000086"
     CDS             complement(98532..99989)
                     /gene="ascB"
                     /locus_tag="IV257_000086"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8813248.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(99986..101881)
                     /locus_tag="IV257_000087"
     CDS             complement(99986..101881)
                     /locus_tag="IV257_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8813249.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(101939..103714)
                     /locus_tag="IV257_000088"
     CDS             complement(101939..103714)
                     /locus_tag="IV257_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8813250.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(103764..105260)
                     /locus_tag="IV257_000089"
     CDS             complement(103764..105260)
                     /locus_tag="IV257_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8813251.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(105454..107076)
                     /locus_tag="IV257_000090"
     CDS             complement(105454..107076)
                     /locus_tag="IV257_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8813252.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(107404..108717)
                     /locus_tag="IV257_000091"
     CDS             complement(107404..108717)
                     /locus_tag="IV257_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8813253.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(108962..109186)
                     /locus_tag="IV257_000092"
     CDS             complement(108962..109186)
                     /locus_tag="IV257_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813254.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(109206..109574)
                     /locus_tag="IV257_000093"
     CDS             complement(109206..109574)
                     /locus_tag="IV257_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8813255.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(109770..110480)
                     /locus_tag="IV257_000094"
     CDS             complement(109770..110480)
                     /locus_tag="IV257_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813256.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(110498..110995)
                     /locus_tag="IV257_000095"
     CDS             complement(110498..110995)
                     /locus_tag="IV257_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8813257.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(111032..111484)
                     /locus_tag="IV257_000096"
     CDS             complement(111032..111484)
                     /locus_tag="IV257_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8813258.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(111619..112224)
                     /locus_tag="IV257_000097"
     CDS             complement(111619..112224)
                     /locus_tag="IV257_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8813259.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(112674..113294)
                     /locus_tag="IV257_000098"
     CDS             complement(112674..113294)
                     /locus_tag="IV257_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8813260.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(113296..114165)
                     /locus_tag="IV257_000099"
     CDS             complement(113296..114165)
                     /locus_tag="IV257_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8813261.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(114188..114595)
                     /locus_tag="IV257_000100"
     CDS             complement(114188..114595)
                     /locus_tag="IV257_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8813262.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(114614..115903)
                     /locus_tag="IV257_000101"
     CDS             complement(114614..115903)
                     /locus_tag="IV257_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8813263.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        33911 a        23950 c        20253 g        37814 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgcttttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggatat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7381 ttttaggaga tcacttaaca agcatcggcg atttttttgt tttgaaatct tcggttattg
     7441 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7501 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7561 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7621 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7681 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7741 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7801 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7861 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7921 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7981 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     8041 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     8101 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     8161 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     8221 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     8281 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8341 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8401 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8461 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8521 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8581 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8641 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8701 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8761 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8821 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8881 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8941 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     9001 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     9061 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     9121 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     9181 agccgatcca aacccttcgg ttcttttttg cctatcagtg caaaagcatt tcctgtgcaa
     9241 tttcacgtcc atccattgta tctggataaa atgttggcca attctccatt tctttcagca
     9301 atacttcttg actttcattt cctgcatata aatgaaaatg ttcggagtca gccggtgcaa
     9361 tcgtgtgatc actgaattga atatatctaa aagcgccact ttccttatct tctgcttcga
     9421 atagatacct tacgccacgg tttcctgcct cgtaggtcaa gatttctttc cctacataac
     9481 gataatttga acgtttcgtt gttccattct taaacgtaaa tgccatctct ttctcactga
     9541 tcttgattga agcaatatct gtttgatagc ccttcgtata atattcttta tattcttgtg
     9601 ctgtcttatc ctgattcaat ttttctttat attcaaaaac aggatcaaaa gtcccatctt
     9661 ctacatacgt atctactgtt tgccaagttc ctgcccaatc tgaaagagag cgatcttgta
     9721 catcctcgtc ttcaaaataa ccgttgtaga cgctcttttc ttttttgggg gtcaaagtat
     9781 cttctagcgg gttttctgtc tctgtcgttt ttttcaacgc ttcgaggttt gcttccatga
     9841 tagaaagata gttttctcca ttttctattt gttcattcgt caatccttcc aaaggattca
     9901 atacttctaa agatacacct gcttcgtttg ctaacgttct ggcaatacta tctttggcgt
     9961 tttcttcaaa ataaatatag ttgattccat gttctttaac aaagtgtttc agttcagcaa
    10021 ttctcgccgc agacggttct tcactagaag aaagaccagc aatcgatact tgattcaatc
    10081 catattctaa cgctagatag ttgaatgcag catgttgcgt gacaaaagat ctttgttttg
    10141 cttgcttaaa ggtttcttga taaagctgat ggagtgcttg aagtttctcg gtataggctg
    10201 ctgcattttt tgtccatact tcctgttttt ctgggtaagc ttcaatcagc tgatcacgta
    10261 tgtttgctac ctgcttgatt gctaaacttg gagctagcca tacatgtggg tctagctcat
    10321 gtgtatggcc ttcttccccg tgttcatggt catgatcatg tccttcttca cttcccggaa
    10381 gtaaaagcat accttctgtc gcttttatcg tatggacatt gccttcttgt aatgtttttt
    10441 ggattgcggg gacccatgtt tccatgtttt catcattata aacaaagata tctgtatctt
    10501 ggatctttgc catgtcttta gcagaaggtt cataatcgtg ggaatcagtc cctgctggaa
    10561 tcaataactc tacttctcct tcatccccga cgattgcttt tgtaaaatca tacatgggat
    10621 aaaaggtcgt catcacgctc aatttttctt tactgtcctc ggctttccca cttgccccgc
    10681 aagcagttaa catacctgct aaaaccagca tggccaatat acctatcaat cgtttcattt
    10741 atgacactcc tcttctcatc ttctttcatt tgaatgttcc gattcgcttt tcgcaaaccg
    10801 taatagttac gttttagcaa caggatttaa tttatcatat ggttaaaaga actgtcaagc
    10861 gttttcttat tttaattgat gagaaagttt atatgaatga ttgacgatat ttttgcaatc
    10921 aacctctatc attttccttt tatacccgct tttgtctata atgaagttat agataaagga
    10981 ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt tgatccgctt tttaggagga
    11041 aaaacctctt actatgtttt aggaatagtt atcttacttg cgctgatcat ttattttttc
    11101 catcagatct cgtttatatt caagcctgtc ggaatcatta tcgctactat cttgccccca
    11161 ttattgtttg cactgatctt gtattatctt ttggaaccag tcatcaaacg tttgagcaag
    11221 cacctttcga ggaacttgtc agtcattatc gtttatgtga ttattttatt tttattgggg
    11281 cttggaagca tatggcttat tccttttctt gaagaacagg caaagacatt gatcagtagt
    11341 ttgccagatt tgtttcaaga ttttcaagag agtctccgac aatttttgga gaaaacacct
    11401 tttgccgatt catttaatca atttttcgct tctattgacg acgtgacgtc tgatatagct
    11461 ggatttattg gaaattattg ggaaagtggc gcacaacgga tcagcaacat tttttcaact
    11521 gttacagcta tttttattac tttatttact ggtccaattg tcgctttttt ccttttgaga
    11581 gatcctcaaa aattttatca gtcagtttta gcgattgtcc caccagcatt tcgtacagat
    11641 tttaaaaatt tgacaaagat tgctaatcaa caattgggtt catttctaaa ggggcaaatc
    11701 attgcttcat ttatccttgg agcagtctac tgggtttgtt tcttactgat cggattagaa
    11761 tttgctagtg ttttagctat ttctgctggg ttgttatgta tcattccata tattgggccg
    11821 tttatcgcct tctttccagg tttgatcatt gcattccaag actctacttt tatggctgtc
    11881 aaatttgtca ttgtctggtt tgccgtacaa cttctccacg gtgacttggt gattcctcga
    11941 gtaatgggtg atcgtttaca gatccatccg attacgatct taatcgtttt acttgtcatg
    12001 ggtgatctaa tgggaattgt gggcgttatt tttggtattc ctatctatac gttagtcaaa
    12061 ttactcgtta cttttatttt ccgtaaattc aaacaacgct acaataaatt ttatggcgat
    12121 aaaggagagt atgaaaactc ttcattttct gaagaagatt attttaaata aaacacaaaa
    12181 cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg tgtttttgtt ttaatttttc
    12241 agcaaagcaa ttgcctggta tggttttagc tccagacttt gatgaagaac agtatcggaa
    12301 taattatcga taagaatatt ggcttctgtg aatccttctg ggatggcgat ccttgtatct
    12361 ttatcataga aatttgttaa tacaagcagc tgctgtcctt caaattggcg aagaaagcca
    12421 taaacttccg gatgatttgc ttcataagca tgataatctc cttgtgcaac gattggatat
    12481 gtttttctca gccgaatcaa tttcttgtaa taggacaaaa tagaatcttc agaagatagt
    12541 tctttttcta cattaatctc ttctagcttg cctccaactt ttaaccaagg tttccctgta
    12601 gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg ttcgagagtt atcgcgagat
    12661 tttgctttga tccgacgaaa cgcttctttt tctgtgaatc cttgttcaag gagtaactta
    12721 taagcattca aactttctac gtcaatatag tcggaaatcg atgaatagtc tgggtcgatc
    12781 atgccgattt cttctcccat ataaatatag ggcgtcccac ggttcaaatg gatcatggtt
    12841 gctagcatct ctgctccttg ttttcgatac tgatcgatag ctacaaatcg attgagcgca
    12901 cgtggctgat catgattatt aaaaaataga gcattccagc cgttaccctc actcatcttt
    12961 tctccccatg tatgaaatag tcgctttaat tctgcaaaat caaatggagg agtagaccat
    13021 ttattcccat tttcataatc gactttcaaa tgatggaaat taaagaccat tgataattct
    13081 tctctactag gttctgtata caagatacaa ttttcgatcg tcgtagagct catttcaccg
    13141 actgtgatga tagtggaatc cgtcccaaac gttgcttggt tcaattcttt tagatacgta
    13201 tgggtgattg gtttgtccgt atattcaggt ttgccatcat tttctttatt atttttcaat
    13261 tgttcgtctt taccgataac attgataaca tcgaaacgga atccttttac ccctttttcc
    13321 atccaaaaac gaacaacttt gaatagttcc tctcttactg cttggttccg ccagttcaag
    13381 tcagcttgtg tccgatcata taagtgcaaa taatatttcc ccgtatctcc aaatggcgac
    13441 caagcacttc caccaaattt tgatacccaa tccgttggct cttctcttaa aatgaagaag
    13501 tcctgataat gctggtcccc tgctagtgct tttttgaacc attcatgttc tgtcgaaacg
    13561 tgattcagca ccatatcgag catgatttct atccggtatt tctttgcttc ggctattagc
    13621 tgttcgaatt cctccatcgt accaaagata ggatcgatgg ctgtgtaatc agagatatca
    13681 taaccgttgt ctttttgagg gcttggataa atagggttta gccaaatcat gtcgatgcct
    13741 agatcattca gatacgggag tttttctttg attcctttta aatctccaat tccatcacca
    13801 ttactgtctt tgtaagattt gggatagatt tggtaaatga ctttgtcttt aaatgtcatg
    13861 gatatttacc tccattcaac tgatttatta cctaccacaa cttatatata caagttatca
    13921 tttattatta taactgaacc gaaagcgttt gtaaaattta tctttataca ttctaagagg
    13981 tctgttgccc aataaaaaac aagagatcag acatgtattt gtctatcctc ttgtttaaaa
    14041 tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa agcttttatc tacggcaatc
    14101 agtcttctca aatgtaaaac atgaggagct aattttattt gagtattctt tttgctcgat
    14161 gcaggacggc ttttttacaa cctctgattc atggtgttca atcagctgtc cttcggtatt
    14221 agctcaaaaa acggcaaaat atgaaaaact attttgccgt ttgtttctct aatactcagg
    14281 tcaaaccgct gatttcacaa cctctgtttt tattcattcg atttcaacgc atcaatcata
    14341 tcgatatgct ttaatttgaa atgggtaaca atcatcacaa taaccgtaaa tacgatagtt
    14401 aatcctgctg ataagacata agcccaccat gtgatcacta atggaaagat gacattctcc
    14461 atagacgctt gttgcaagat gaagtcagtc aatatatatc ctacaccaaa tcctccgatg
    14521 attcctaaaa gagtaaagat gatattttct cgaacaatgt acatcgtgac ttctttatca
    14581 aaaaatccaa ggacttttat tgtagataat tcgcgtacac gctcggaaat attgatatta
    14641 gttagattat acaagacgat aaaagccagc agtcctgata atacgacaaa gattaagaca
    14701 accgagtcta agttcgccat tgattcttct tgcgtttcga tttgtttgga catgaaagac
    14761 gtatttgtca cagcgtctgt ggcaagcagt ttctcggaaa gcatttcttc ttcttttgtt
    14821 gtcatcttat ctgtacgcag caaaaatgca ttatccatca tcgattgatc agtaactgaa
    14881 gcataatatt caggggacat ataaataaag ctacctagat agtttttcac gatttctttt
    14941 acttcgactt tccattcttt ttgatcttca tcataaagcg aaatatgatc tcctgtagat
    15001 aagtcaaata gttctgctac tttttgcgta atagcgattc ctgttttagg caaggagcgt
    15061 gggttctctt caagatcagt taaatggata aaacgatcca aatcagctgt tttacttggt
    15121 acgatcactg tcagactttg cgaagcatgt cctttttttc tcaattctac ggtttgagta
    15181 tgaatagcta ggatctcatt tactttttct tgcttctgca gcatatccgc tgctttttga
    15241 cgactatctt ccgtggatga atccaatgta acgattgctt gatagtcaat gatcgttcca
    15301 aattgtttgg ctgaaacaga acttagagaa tctttcaatc ccacgcctgc aaccatcagc
    15361 ccggcacatc cagcaattcc gataatcgcc ataagcattc ttgacttata tcggaagaga
    15421 tttcggtaac tgattttttg attaaagcta aggcgtgacc acagcggagt gatgtattca
    15481 agaaaaatgc gtttccctgg cttcggtgct ttcggttgaa gcaatgcagc aggccgttct
    15541 cttaaatctt tgaataaaac gatcattgca gcgccaaatg cggcaatgaa gaatgcggca
    15601 gctgcttgta aaatcggcgg ccagtcataa aaaataagcg cactcccaat atcataccgc
    15661 tcgttagaca gctcaaaaat gatccttggc aaaagattcg ttcctaatat cgttcctaaa
    15721 acaataccta tccctgaggc tagacttgca tagattgcat atttttttgc gatttcaact
    15781 ttcgtataac ctaacgcctt taatgtcccg atttccctgc ggttttcttc taccatacgt
    15841 gtcattgtcg taaaggtgat caatgcggca ataaagaaaa agaaaacagg aaaaacattg
    15901 gcaatcgcag cgatacgatc agacagatca ccaaattctt gaaaaccgac attttctgaa
    15961 cgttcattgt ataggtatga tggtttagtt aaattttcaa ttttttctgc atttcgattc
    16021 aattcttcct ctgcagaggc cagttcacgt tctttctcaa ctaattgcct ttcttgttca
    16081 gcaagttgag cttcttgttg agataatgtt tgaacagcga ttgttcgttg cgcttcagga
    16141 agctgctgga tagttgctga ctgttgcgct aactgttttt tagcatcttc taaccgtgtt
    16201 cttgcttgcg ttgtttgtgc tttgccatcc gctatttttt gctgtgccgg ttctaattct
    16261 tcgttggccg attttttcag ctcttctaag cgttcttcgg gtcgatcttg taaatacttt
    16321 tctgcctttt cttgattttg ctccatttta tctttgtaag ctttgctgta cgtattcaaa
    16381 ttctttacgt ttgtaaaatc aagatataag acagaataaa tatctgtttt aaaaacggat
    16441 tcaggtaaat agacaaaata atcgacgcta ccatttccga cgttcgcgta tcctctctcc
    16501 aaattgttga tgaacaatgg agaacggaca aagcccgtga tggtatactc tttttctttc
    16561 aattcatctg aatcaatctg ataaatatca ccgattttat acccttcatc ttccgctttt
    16621 tcatccaata caagctggtt tggtttttcc gggagttttc catcagttaa ttgtagcttg
    16681 ttctgcttgt tatttttgtt ataagaaaat atttgaatca cttcgttttt gccagtattg
    16741 gaataataga tctgttttcc tgtttctact tttgcatctg ggatcttttc tgcttgagca
    16801 atatcttttt gtgtcaagcc accagtagaa acgatttgca aatcagaaag tcctgctgta
    16861 ccaacatagt gatccatcgt tttttgcatg acaggtccag cagttttgac acctacaaat
    16921 aaaagtgttc ctagcatgac aataaggatg attgcaatga atcgtccttt agattgccga
    16981 atttcgcgaa aacctgcttt aagatatgtt cttgttttca taaaatcccc ctaccataca
    17041 atctcatcaa tggatgaagg atgatcattt aattcaacag accttacttt agcatcatta
    17101 atgtgaatga ctctatcagc gatgggcgct aaagcagaat tatgtgtaat gatcaaaacg
    17161 gtattgccgt gttgtctgct agcgttttgc aataatttca atacttgctt gcccgtttca
    17221 aaatccagcg cgcctgtcgg ttcatcacat aaaagaagtt tgggattttt agctagtgca
    17281 cgagcaattg aaaccctctg ctgttcgcct cctgataatt gggacggaaa gttattcaat
    17341 cgatgagcta ggcccacttg cctcaatacc tctacaggat caagcgcatc aggagaaact
    17401 tccgttgcca attccacatt ttcttttgct gttagattag gaacgagatt gtagaattgg
    17461 aaaacgaacc ctacatctgt tcgtcggtaa gcagtaagct gtttatctga aaattgcgca
    17521 atatctgtgt catcaataat gatctgacct tcatcaggtg tatccatccc acctaaaata
    17581 ttaagaatcg ttgactttcc tgctccactt ggaccaagta taatcactaa ttcgcctttt
    17641 tcaattgaaa aagaaatatc gtcatttgct gtaatggtcg tttctcccat tttgtatcgt
    17701 ttgtattcat tttttacttc gacatacttc actctgattc ctccattttc acgatatttc
    17761 tcgctatatc tatgcatcga atgcgttttc tcacacttat ttattttacc actgaatttc
    17821 tctagactaa agaaaggaat cctttaattt agttgacaaa taaacgattt atgatttact
    17881 tacataaaaa acattgttat ttgaaagaag gaacatcatg aatgatttaa aggaaaatat
    17941 gatttttgat ttatcaaaaa ctgcttttgt cgcgatcgac atgcaaaaag agatcgtaaa
    18001 taacggtaca ctctctccac acactgcagc atctattctg acaaacaatg atttgttagt
    18061 aagaacattg aaacgtaccg ctgccttgaa agttttagtg aacgtagaca tctccacttt
    18121 cccttatttg agtcaacaaa ctgatatggg tggaatgact gcttctgtac ctccagaatt
    18181 cactgattta cttttgaagg attctttgaa agatacagat aatatgttga ctatcacaaa
    18241 atacaatcca agtgcttttt ttggtacttc tcttgatctt caacttcgcc ggcgtggaat
    18301 cgaaactatc attttaagcg gtgtagcgac aacaaacggt gtatatgcta ctgcattaga
    18361 tgcctttcaa cacgggtacc atatcgtgct tgctgaagat gcttgcagtg atcgtgacaa
    18421 agaatcccat caattgttca taaaaaagat atttccgaaa actgcacgag tacgctcaac
    18481 gaaacaaata atcgaagcta tccaacaaag caaataagta ctggcacacg tgattttcat
    18541 ttacttcttt ttgatttcat gggaaaataa ccacaggatt tagataggag tatgaacatg
    18601 gtgaaaaaaa agatcacaat caaagaagtc gctaaacttt ccggcgtttc aatcacaact
    18661 gtctccttga tattaaatgg aaatacggag aaattcagtc cgaaaactgt tcagaaagtt
    18721 ttagctgcca aagaagaatt aggctatcag cctgactatt ttgctagaca aatgattacc
    18781 aaggaaacga aaacaattgg tgtactcgtt cctgatatca cgaatccatt cttcagtaca
    18841 ctgatgcgag gaattgagga tatcttgtat aagcagaatt tcgtcacgat attatgtaat
    18901 gctgattctg atcatcaaaa agaaatcgaa tatctagccg agctcacacg tcgaggtgtg
    18961 gatggattca ttatcgcgac ttctgctgtc tctacagatg caatcaacga aaatttgaaa
    19021 aaacaaggcc ggcccttcat tgttttggat cagaaaaaat ctgaaggatt tagtgatgcc
    19081 gtacgaacag acgattttcg cggtggttac ctagcgggca tgcacttgct tagtctagga
    19141 catcaaacga tcgcccttgt ctatcctgaa aatccaccgg aaaatgtcca cgctcggatt
    19201 gaggggttta aatctgctct tgatgtttac caaatccctc acgaccaact gatcctctta
    19261 cctacacagt tctcaaaaca aggtggctac cagatcacag cagaacttct tgattctgct
    19321 gcaactggtg tgtttgcttt aaatgatgaa ttagcttttg gattatatcg cggattagag
    19381 gaagctggaa aaagcatccc tgaagactac agcattattg gctatgataa tatagatatg
    19441 tgcgagtata tcaagccgaa actgacaacg atcgcacaac caatcttcga attgggacaa
    19501 acatctgcca agttattgtt agatcggatc caattcccag aaaaagaatg ggaagaaaaa
    19561 agattacctg tacgattaga aaaaagattt tcaactgcac ccttgaaata gctttcaagg
    19621 tgtgctatat ttttatcgaa ctattaaaac gttttactaa aacgttttaa aaaataattt
    19681 tcccatcaaa ataaatgaga tagttggagg tttgtaaaat gaacacggtt acagtaattg
    19741 gaagtattaa tatggacact acccttcgtt taaccaacat gcccaagcca ggcgaaacga
    19801 tgcatgcgca tgagattttt cacgctggcg gaggtaaagg tgccaaccaa gcagtggcag
    19861 ccaaacgttc aggcgcacgg acatctttta ttggaggcgt tggggctgac agcgaaggac
    19921 aacagttatt agatctactg acaaaagaaa acatcgatac atctgggata gctgaaatcc
    19981 aaggcgccac aaccggacaa gcaatgatta tggtagacgc agcaggagaa aatagtatct
    20041 tgatccatgc aggggcaaat aatgctttcc atgaacaaga agtcctaaaa aacaagcaac
    20101 tgattacaaa cagtgacttt attattgctc agtttgaaag tagcttagat gcgacgatcc
    20161 tagctttttc tattgcaaaa gacgcaggaa aaacaacgat cttgaatcct gcaccagctc
    20221 gtgaaacgat tcctactgaa ctacttgaaa agacagatat cattatacca aatgaaacag
    20281 aaacagaaat cattacagga atcagagtga cagatcataa cagtttggtc gcagcagccg
    20341 aaaaactcca tgaattaggt attggaacag taatcattac actaggcagt gccggcgctt
    20401 tctatcatac agaaaaagaa cacggtatcg tccctgcttt taaagtggat gccgttgaca
    20461 caacagccgc aggtgataca tttattggcg cattaagcag cacacttcag ccagacctta
    20521 gtaacttgaa agaagcgatc ctttatggaa atctagcctc ttctgttgct gtgcagtctt
    20581 atggtgcgca accttctatc ccttaccgcg aagctttaga acaatcagca gtctaaaatt
    20641 aacaatgaga ggaagaaaca aacatgaaaa aaggaaaagt aatcaattca gatatttcac
    20701 gagtaatcgc acaaatgggg cactttgata aattaagtat cggggatgca ggcatgcctg
    20761 tcccctttgg tactgaaaaa atagatttgg ctgtctcaaa cggtcttcca agttttttat
    20821 ctgttttaga aaacgtttta gaagaacttg aggtacaatc gattgaattg gcggaagaaa
    20881 taaagacaat gaacccggaa atattggaaa aaatcaaatt attgttacca gatacaccga
    20941 ttagctttat cccacatgaa gaaatgaaaa aagagttgaa taactgtcat gcatttattc
    21001 gtacaggcga aatgacacct tacgccaata ttatattagt gagtggagtg gtcttctaat
    21061 ggaaacgaca atgaatcctg aacagatggt actgtccgag cagattcata tgctgattga
    21121 acagttggga ggaaaagaca atattgattc tgtccttcca tgtgcgactc ggcttcgtat
    21181 caaattgaaa aaacagccag tagccaatgc agaaaaatta aaacagcaaa tcccttccat
    21241 taagtatttc attttactcg atagatatgc agaaataatc atggatcagg gattggctcc
    21301 tgcttacaag gaattgacgc gtcaaatcga tcattcagct ccgaaagaaa agctaaatcc
    21361 gatcgaaaaa ctatttggtt tgatatcgga aatctttacg ccgatcattc cgctgtttgc
    21421 cggttctggt atattaaaag gtctggttgt tttagcgact acgattggct ggcttagtga
    21481 aacaagcgga acttatcata ttttgtctgc tgcgtcgaat gccgtctttt attttctgcc
    21541 aatctttctt gctttttctg ctgccaaaac gtttaaagtg aatggatata ttgcggctgt
    21601 cattgctgca gcactgattg aaccgaatct gacctcttta ctttcagaca gtggcaaagc
    21661 agctactgat tttctgggga ttcctgtagt actgatgcag tatacgtcta ctgtcatgcc
    21721 ggcgatttta ggtattttct tttattcttt tgttgaaaaa ttcctcaaaa aatatattcg
    21781 cgaaaatatg caattagtat ttgtaccttt actttcatta gttatcacgg ttcctttgac
    21841 gttaatggta ttcgggcctg cgggtgttta ccttggcgaa ggtttagctg ccgcaattac
    21901 ttggatgatg gatattaacg gaccgatcag tggcgcaatc atcgctggtg gttggaatat
    21961 tttggtaata tttggtatcc aatgggctgt aaaccctgtc atgatcagta atatctctgc
    22021 ttatgggttc gaccgaatcg ttccgttgac aggagcagcc aattttggaa tggctggtgc
    22081 agctttaggt gtatttctgc gaagtaaacg ttcaaaaacc cgttcgatct ccggttctgc
    22141 ttttgcttcg attttacttg cgggagtgac cgaaccaact gtttacggaa ttgcgatccc
    22201 attgaaaaaa ccatttgttg ctgcttgcat tggtgcagct gctggtggag ctgtaatggg
    22261 atttgcccaa gtgaaagcga tcgcttttgt atttggtagt ctgaccacat tgcctgcatt
    22321 catttccggt acttttttct ggtacttagc aggactggct gttagtctag tcgtagcaat
    22381 gatcactact ctcgttagcg ggtttgatga agacctaatg tcttatgaat agttaatgaa
    22441 aaagactgaa gtatgagatg tttatcacat atttcagtct ttttcttttt aggatcatta
    22501 tttttataaa tttttgtgat actttccaaa ctttggtata atttacatat gcataaccat
    22561 ttttatcccc acttgtttat ccccaaacaa aggtggtcta tgcaataaaa tgaacgtcaa
    22621 agaaaaacga atgaatgctc gttgcttatt tacaaattgt tttatttaga atacacagac
    22681 agatttatga aaagaaaaag cttttcgaca cgattatctg ttccaaaaaa tgtctaatga
    22741 atgctgaata agacagaaaa tcaatgaaag gaaaaggact agtttattat gaaaaataaa
    22801 aaaacgatac gtttgctagc tgctacttgg ctgattgctc cgtctgtttt gtcgaccaat
    22861 accgtatttg ccgtagataa tcaaatagct gatcaaacag tcacaacaga tacgactaca
    22921 tccagctcag ctactagtga aacaacggat tcttttacag aagacccaac tggaacaact
    22981 aactcaacaa caggatcgac cgagccaact gaaattgatc cggctgatcc atcagaaaat
    23041 gaaaagccag ccgagacata ttcccttaca attgctcctt ctttgattgg taagatccaa
    23101 ctgactgctt cgacagcgga ttcccctgaa cagcaacctt tggatattac ggaaagtgga
    23161 acggtagaag gtttattagc ggaaacgaag gtaacttaca ctattacgcc agctgaaggt
    23221 gaacaaattg cttcagtcgc tgttaatggt gccaaagaag taaattatga tgcaaagacc
    23281 tttacagtag gtacgaacaa tttatctatt actgcagaat ttaaagctgg accggatact
    23341 tcaacagaga aaccaactga tcctagtgaa gaagtcccta gtacaccaga tccggaaacg
    23401 ccaaacaaac cagatgtggg taataacaac aataataatg gttcaaataa ttcaaacaat
    23461 aataacaata attcgaacag cgggaattct tcgaatcaaa caaacccatc tggatcgaat
    23521 aacaatggaa atactcagag agaaccaaac catccaaaca atggttcatc gaatggcgga
    23581 acgaacaatg cttcatctcg tccaaataca gcaaatcaaa gcggtataga gaatccatct
    23641 tctgcttctt ctgattttgt tgtaaaatca ccaattgatg ctgtattgcc tacaaataca
    23701 acgaatgtcc aacaagcaat cgttcgagaa gcattcaatc atcttggaaa accatatgta
    23761 tggggagcaa aaggtccaaa tacttttgac tgttctggtt tgacttatta cgtatatatg
    23821 aaagcaaccg gtcattatat cggtggctgg acaggcgaac agcaatatgc aggtacgcag
    23881 attccagtaa gtcaggccca acctggcgac ctcgtcttct ggggaccttc ttccggtgtt
    23941 actcaccatg taggtatcta tattgggaac ggacaattta tccatgcgcc tcaaccagga
    24001 gataaagttc gagtaacttc tatttcagat ttcacgcctg attttgctgt ccgagtaaac
    24061 ttagcaggtt tacctgctgc ttcaggttcg ctgacaggcg gctcatctat tttagatgga
    24121 ttagatggct cgttccattt cagtcaaaac caaacaactg atcagttctt gaaaaagatt
    24181 gcagatgatg cacaggaaat cgggcaaaaa gaaggaatct acgcatcagt catgatggct
    24241 caagctatcc ttgaaagtgg atctggaaac agcttattat caagcgaacc aaatcataac
    24301 ctttttggta tcaaaggatc atataaagga agcagcgtga ccttcaatac acttgaacaa
    24361 gatagttccg gacaaagcta ccaaatccgc gcacaattcc gtaaatatcc ttcttacaag
    24421 gaaagtttgg aagactatgc cgacttgatc aaaaatggac tgactggcaa tcctgatttc
    24481 tacaaaccaa cttggaagtc ggaaacaaaa gactacaaag aagcaaccaa atatttggaa
    24541 ggacgttatg ctacagatcg ccaatattca caaaaattga acgcgatcat cgaagcgtac
    24601 gacctgacaa aatatgatga accgaaaaaa gaagcagcaa caactgaaga agcaaacgct
    24661 gaaatcctca atatgtctaa gcgattcgat gtgccaatca aatggacaac agatggcgtg
    24721 ccaatcacag gattagaatt ttcaacgata aaacgcgtca acaacgcttc ttccatcttc
    24781 cgtgtcgctt caatctggga tttgtggaac aattttacgg caagacagat accagaaaca
    24841 actactcgta cagttaaagc tgcaactgtt cctgtactaa ccttgttaag tttagacaaa
    24901 tcattctggc ttgatctgcc tttaaagaaa tagaataaat taaaaagggg atgtgacact
    24961 gtcacactcc ctttttaatt tattctattt ctttatattt attttgtatt ccgttacaaa
    25021 ctcgcgtact tggttaggtt ccagagtgac atctccaaaa ctttcaagtc gctccgctcc
    25081 tggcagttct tgagtctcaa atgtgatacc gccatgattt actaatttat tcccgcgcat
    25141 ttccggtcca tcttctccaa aatttgctgt aaaaatcacg attcctgaag catctgtttt
    25201 tacttggatc gatacttttt catctgggct tgtcaattcc gcagcattta gtaaaccaga
    25261 ttgttttttc aacaaaaatg gatgatcgat gccgcccact atcattgatt ggcgaaaatc
    25321 actggcaaag acctcaccta attttttagg acgttggaag tcaaatgctg tgccacttac
    25381 tgaaactact tctcctgtag gcgttgtatc tttattgagc gtagcaaata atcggctatt
    25441 caaccaaagt atatgttcgt cgattgactg tgctggatca ccggttaaat tgaaatagac
    25501 atgatttgta ggattgaata ctgttttgcg gtcactgatc ccttggaccg tcactcgcca
    25561 tgtatcatcg ttcgtcagcg tataccgtac ttctacatct aaagttccag gaaatccgtt
    25621 ttccatatct gggctagtct gatagaaaat gaccgatgct tcgttttcac cattgatgac
    25681 tgtataatgc cactgcttgg cttcaaagct aggtgcacca ccatgtaaac aatgaccagt
    25741 gtccgggtct gtttttactt cgaacgtttc atcatctaat ttgaatgtgc cattcgtaat
    25801 ccgtccagcc atacgtccga ttgaagcacc gatataaggg tctttttcta gatattcttt
    25861 tgcttcatca aaacctaaga tcaactctcg tttttcatta tcgattattg tttgaaaatg
    25921 aacaacccgt gccccaagat cactgacaga caaagatgtc ccattttgat tcgtgatcgt
    25981 aatcaagtga tagttttcgc caaaattttg ttcattgatt tccatgttcc ttgcaccctc
    26041 ttttctttca tttgcactga ttccagcata catggaaacc gttttcttaa cagcttattc
    26101 cgtgagtaac gagaaaaaat ttactatatt ctaataaaag accgtgaaaa aaaggttgtg
    26161 acaaaaatat tgtcacaacc tctttttccg aacaaacggt gttcaaaagc ttgcatctgc
    26221 ggtaataagc ccaaccaaac gaaaaatatg aggagctatt tttgcttggc tgttcttatt
    26281 acttgatgca ggacagcttt ttcacaatct ctttttcaat acgattccag tatataacca
    26341 tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat atcgtaaacg gaacccaaat
    26401 atctttgtat ggattttgca tgaatagacc aaacatttgt aatccgaata agacatggac
    26461 tacacctaag attcctggtg ctaagaataa cacgcctact tctctacgga tcgattgttt
    26521 aagcaacccg cgtcgtgttc ctatcttttc aagcatttca taacgagcaa tgtcactctt
    26581 gcttcctgac aagatcttga acatcaaaca gctagcaagc atggtcaaga aagctaaacc
    26641 taggaagaat cccataaatt caaaaccaga gaatagacca ttatacactt catacatttc
    26701 atatttctgg ttgctgtact ctactttttg caaagatgga ttgttcattt tattttgagc
    26761 aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct tttacctgaa ctagctgtaa
    26821 agtggattgt ggcaagttca gttgatcaaa ttctgattga ctgactacct tgatttcttt
    26881 ttcttgctgt tccggcaatt catagcttcg aagttcctct tgtacagtca gttcattcat
    26941 ttttttagca ggaacttttt ctttcgtgat cttcggcggt gttccatctt catgttttat
    27001 catgatcaat ggttgagccg caaattcact tgcattatag taaatcgttc ccgcatcttc
    27061 tttttgagta taagaagcag aaagtgttgg tgaaagttct ttgatttttt gctgatcttc
    27121 tgcccgagga ttattcatca ctagatcata tccagaattg ttttctgtca agtctgcaat
    27181 ttcattttta aaacctaatc ctaccgtcaa agcgcccagt gccaaggcaa acagcatagc
    27241 gaccattgag agcatttgtg tatattcacg aatacgaaag tttaactgtg acaatgtgaa
    27301 attgttcaat tttttcaatg aaatctgttc tgatttcttt agtaatgata aaaagaaaat
    27361 gatcacagaa tggaaaatga aataagttcc taatacgatc gtaactaagg ctacccctat
    27421 accaaagact tgataggtcc ccacgtgttt catcatatag taaccgcagc ctagaaaagc
    27481 aattcccagt actacttcaa ggaaaaacaa gaatttatgc tgcttgattc ttgttggtgt
    27541 ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca atcgaaaaag cattgacaac
    27601 agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg atcaacaacc cgtgaatact
    27661 aaaaggtgta aaatgcgaaa tattgagatc taactgatcc actaataatc ggtggacgat
    27721 acttgtcaat cctacaccga cagctgaacc cactaaagta gctgcgatcc ctactaagaa
    27781 tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt gctcccagca tcataaacat
    27841 tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt gcatataaaa tataaacgaa
    27901 tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg ataactgtag ccgcaacgat
    27961 cgtattactt ttcaaaaaag cttcgttgct tgccattgat tcaaacatgt aaaaaatagc
    28021 agatgccata actaatcctg agaataacac caagtagtct cttaaacggc tcttgatccc
    28081 agtaagtgat aatttccaaa gcatagtcaa tctcccccta ctgttccaag tttccaagaa
    28141 tagatagtat ttctcgatag aaggaatcac ggctttgatc tccacgtttc acttcttgat
    28201 ggataccgcc atccttgatg aataaaatac gttggcaata acttgcagaa aacggatcat
    28261 gcgtcacaag aagaatcgat acatgatctt ttgtattcaa ttcgtccatc gtatctaaaa
    28321 gatcccgggc acttttggaa tccagcgcac ctgtaggctc atcccctaat agaatcgttg
    28381 gttgtgtgat caatgcacgt gcggcggcga cccgttgttt ttgtccaccg gaaatctcag
    28441 aaggatagct ttccaaaata tgagagatgg ataaacgttc tgcaattttt tggacacgtt
    28501 gcttgatttc ttttggtctg acgccttgta aagaaagagg aacagcgata ttttcttgtg
    28561 ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa gccaatctca ttcgcacgaa
    28621 aatctgctag ttgatttcct tttaatgtgg tcacatcttt ttgattgatt cggatatgcc
    28681 catctgttgg tttatctaat gtggataaaa tattcaaaag tgtcgactta ccagaaccag
    28741 aagcgcccat gatcccaata aattcgcctt tttctacatc gaagctgatg ccgcttaatg
    28801 cttgcgtctt tttctcattg ttttttccgt atacttttgt tacattctgt acttcgacta
    28861 ttttgttcat ttctttccct accctttaat tagtttttcc agtgctgttt tcggcacgtc
    28921 ttttttttcg acttcttcat acgaagtgac acctttattt ttgttccagg tgatttttaa
    28981 atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg aagtccagtt ctttttcctt
    29041 accctctttc gtatagccag gcaggatata tctatagttg actgtttctt gtccagagtc
    29101 tgattttgaa actgtttttg taccatctgt cgtaatttgt acatagtatt cttctccacc
    29161 cataactacg taatctgcga ttttcacccc tgcaagtacc acaacgatga aaacaagtag
    29221 accgatcaga agttttttca ctttttcccc ttctctctta ctttatatac tgattataga
    29281 aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct tctttcaatt ttgtcatttt
    29341 ctttaaaaca actaaaaaaa gagttcaaga caataatccg tcctaaactc cttcttccta
    29401 tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc tgtattgata acaggttgcg
    29461 ttcctttgtc tgtaatgatt gagacaagta aattattgat caattggact ttccgatcat
    29521 cggtgaaatt gatttcttgt ccatcttcga tttgctctaa ggccatttga gtgattgtga
    29581 cagctccttc aacaatggtt tgtcgagcag acaaaatggc ttttgcttgt tgacgttgga
    29641 gcatggcact agcgatttct gttgcatagg ctaaatggtt caagcgtgtt tcgattactt
    29701 cgacacctgc tactgctaaa cgctcttgca gctctttttg caactcgtca gacactgccg
    29761 tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt atcatacggg tattggctag
    29821 cgatatgccg aatggctgtc tcactttgga tttctacaaa atcttgataa tacgctacat
    29881 cgaataatgc ctttgctgta tcaacgactt taaagactac tactgctgag atttcgatgg
    29941 ggtttccatc caaatcattg acttttaaaa cagaactatt gaaattccga actttcaacg
    30001 aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa gccattttct cgaatcgtac
    30061 ctaaatattg tccaaaaaac aaaattactt ttgcttgatt aggactgact acagttgccg
    30121 aacttaacaa caataacgtg atcaaccaaa gaaaaatact taaaaaaagt gcccaaactt
    30181 tcgcttgcca catacctaaa tagaagaaaa acaatccaac taaagctaat acgaccaatc
    30241 cgacaatccc tgcataacca ttgatataaa atgctctttt ttctctcatg ctacttcctg
    30301 cgctccttta tccttcaata aatatctaat gttacttttt taattttttt ttcgaatacg
    30361 cgccattaca ccttctagtt ctaactcttt gtcttcagtg atgtaccaac cgcttgcctg
    30421 tgtccctccg gctgcctgcg gtaaataatc caattttgtt cgaacccagt gcttttcttc
    30481 tgtaaaatag tcttctacca agacctctag atccatccct gctgaaagag gcaaatcttc
    30541 ccgaacagat ttaaagataa atcgttcctt cttcccatca tacttcaata ttcctgtatc
    30601 gataatcggt aaagaataat aatccagctc tttcttcacc gtcaataaat gtttgaaaac
    30661 ttcatggtac acttgctcta attggcgacc ttctgaattg ttcgggtaat taaccatcac
    30721 tttttctttg tctggatcat agaaggtttg gcttagttga gtgatataag tatgtaatct
    30781 ttccatttct gtttttaact tttctctttg accttcttct accttcatat actctttcac
    30841 cctgttttct ttttagatat tttctttctt attttactat agttatgtgc gttaagcgat
    30901 gaaaaacagc aaagattgga aggatctttg ctgttttttc ttaaaatatc cagttgagta
    30961 caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa tattgatcga tgtgtcttta
    31021 gcagtgatct ttagcttttt ctgtccttgt tctaacaaag tatttgttat tggctggtag
    31081 tctttttcaa tataagattc accagacgtt tggatagtat agccactgtt ttccatctca
    31141 tgcaaagata aattgccacc gtttaggatg acttttcctt ctttgctgat caaactgtct
    31201 tctagatgga agttgctgtt ttcacctgtg acgatcaagt tatccacatt ggcctcatct
    31261 aatcgaagtg agcctaattc atctttcgca gttattgttc ctcctgtaaa ttcattgaac
    31321 gatacccagc catcatgtgt cttagtattc acttttttga ttgatacttc gttgatatca
    31381 attgcactat tattcccttc gattgtcaat tcagataatt catgatctgc agggacaaca
    31441 atagtgattt gtgcacttgt gtcttctaaa ctaatgattg cacctgttga ttttttgact
    31501 gcattcacag tcaacacgcc atttttctcc tttatttctg gcttttctgt cttatcataa
    31561 gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa ctgtttgatt ttctgcatgg
    31621 atgacgattt tgtttgcatc cttgattttt tttgtctcag taaccatatc gatcaaccga
    31681 ggaccattat cccaagtcag actcttttga gcgcctaatg tataagttgc acctgctagg
    31741 aggccaccag taagcatcag accaataccg gtaatcaaaa aatgtttcaa tctcaattta
    31801 tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt ttgtaataaa acgtcgtccc
    31861 atccatttaa caaagttcat catgacataa aaaagccctt ttgttccttt cagcaacagt
    31921 ggcgaaataa atactgcgct tcctgtggcg aacagaccga caccgataaa gaataatcca
    31981 ctcagaatcg attgtccaaa cacactgatt cctgagatga tcatgaatag gccaacacct
    32041 aagatactga tacataaaac atagatacta aaaatgagcg ctagtaaagc aaccaatagt
    32101 cccccaataa ccaatataaa ccccagtgct agtggaatga ggacaggaga agcaaataaa
    32161 ccgataatga ttaaccaaat caggcgaatt tgtctttttg gatttttcac taactcgttt
    32221 tctgcttgtt cttcttctga aaagtaactg atttttaatt ttaaggcaaa ctgctttggt
    32281 gtaccatatt catcaatgat ctgatctaat gtttttccag aatctaaaaa atattcctca
    32341 taatactgga tcaaatcaaa acgatcgtct tcaggaattt cttgaagatg atccacaact
    32401 tcattcaaat attcattcat ttgtgctctc ctccaataac cggtctaaag aatctcgaaa
    32461 aacaatccat tcatcaataa tttcttctaa atgacgactt cccattggtg tcaacgagta
    32521 atatcgacgg tttctgcctt caaaaggttc atcataggtc tcaagatatc cttctttttt
    32581 caatcttctc aaaaccggat atagtgtaga ttcactgaca gagagacttt cttgtacgat
    32641 ttttgttaag gcatacccat acatatcttc cttgcttaat gctgctagca cagtaccatc
    32701 aagaagaaca gttggaatct ggatgtgcat tacgtcacct tctattctca agtgtaatat
    32761 tttatttcaa tctatattat acaacatata atatagtttc aaaatcagtt tgtcaaatac
    32821 aaaaaaactg actaaaagat ttgatccttt ttagtcagca aaaaatggct tctctttgat
    32881 ttttattata acagtggcat aatccaattt gtccacatcc agctcagtgg catgataacg
    32941 atcattgcag gtatcatgtc agctactggg aacattttca cgttgatcat tctaaaaccg
    33001 gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta agatcatgtc tggtgtagtc
    33061 aatggataaa taaattttgc caataagaac aaaatataaa aaataatgaa ttggggaatt
    33121 gctatcacag aaacgacata ccctaaatta cacgcaaaaa tagctgctgt gaaaaaatca
    33181 aggacagatt tagaaatcaa aatcgtactg tctcctgtca tcccagaatc taaacttcca
    33241 tagatccccg taccacttgc acaaaataga acaatgaccg tcactaatgt gctgataaat
    33301 tcatcgtggg tcataccaag cttttcacta gggaagattt tagaaatagg tacttgcatc
    33361 agcgcagccc ccttatttat cccattacct aaatgaacaa tcaaaccaat cgctgttcct
    33421 atcactaaag caaaaataac agccggcata tttttcatcg gcgcaatgga atagattccc
    33481 atccccatcg aacacacacc aaaaaccatc gtcagctgtg ctttaaaatg ttcacttaat
    33541 ttatttccta ctaatccgcc aattagtccg ccaaacaaaa cggaacctac attgatcaaa
    33601 actccagttg gcatcaaaaa actcctctct ttttctacga agcgaaaaaa atcagaaaaa
    33661 tttctgtaat tattgctttc gctgcaagaa agattgtaac agacagagaa gattacacca
    33721 attcattttt tgtgttaaag tattctttat tggaataagt aggaggaata tgatggatat
    33781 ccataaattc gaagtttttc ttgatttagc cgaaacaatg aatttcacca aaactgctga
    33841 tcgacaattc accacccaag gaaatatttc taaacagatt ctttcgttag aaacagaatt
    33901 agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa ttgaccgaag ctggaagctt
    33961 attgctccca tatgtaaaaa atgtcgtaga acaataccat gctatgcaag aagtgcttga
    34021 aacctatacg aaagacaaca atctttcatt aaatatctta acgataccga caatgatcaa
    34081 ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc catcctgaat ttacagtcca
    34141 gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac gccaatgaac atagtgacac
    34201 gatccacttt gctagaagtt tccaggcccc accaagttcg attgagtggt tgcctactga
    34261 aacagacgat tttgtagcag ttctacctaa aaatcatcct ttagctagta aaaagaaatt
    34321 ggatctaagc gaactgaaaa aggagaactt tttgctcctt ggccccacta cgaacttgta
    34381 tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa ccaaaaatct cttataaagg
    34441 agcaagaatc gacttgatca tcaatatgat tgcaaatgac atggggattg ctattgttat
    34501 ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg attctcccta tttctccaac
    34561 aacagagagt tatcttgcat ttactcgtaa agtcggtgag cattctttgg cttcagatac
    34621 attttgggag tatttaaatc aagaagaaaa ctaagcccta cattttctgt ccatctatcc
    34681 acccaaaaaa agagagcgac gcatgacttt cgctctcttt cttgcatcat gtccttgttt
    34741 caatgacacc tctaacattt ctcgtagttc aataagaaag cagcgatttt tatgccaaaa
    34801 gaaccgtaag aacatttccc taatcacaaa aaaacgacta aaaaccccta aaaaccttca
    34861 atagaatttt taggggtttt attctttttc ctattgttta cttaaccgta tcattgctgt
    34921 cgttcctact ccttcgatac tgctataatc aatacgtcca tcatgcagtt ctagaatttt
    34981 tttaaccagt gaaagtccta gcccattccc agaaatttga cgacttgtat ctccttgata
    35041 aaatttttca aacattcgat ctctggtctc ttcattcatt cctacacctg aatcggtcac
    35101 ttccagtaca atctctgtgg ctgtctcaaa tagtttgata tgaatctgtc cattgatttg
    35161 attgtacttt atggcgttat ccatgatatt cagccacact tggtacaaaa actcttcatt
    35221 gcctgtgtaa tttactgcag caagttcgat atccaattct agctgttctt tctcccattt
    35281 aggctgaaaa aaaacgatta cttctctgat ttgttcatct aatcgatact ctttcttttc
    35341 cagttggata cgctggtttt ccagtttgtt caattttaag atgttctctg ttaattgtga
    35401 aagctgcgtg atactttcga taatccgata taaaaatatt tggcgttctt cgtcagagag
    35461 atttggggct tgtaacaatt gaacatatcc ttggattgtt gctagtggtg ttttgaattc
    35521 atgggataca ctggacacaa agtcattcct cagtgtctcg atactattga gttcttggac
    35581 cataacgttg aaatctttat acagttgctg tacttctgct actttctgct gttcatccat
    35641 tcgaatcgaa aaatctcccg ttgctaccag actcatattt gtccctagcg cactgattgg
    35701 atgcaggata cgccgcccaa caaaaatcga tattcctgta cccacgacca tactgaaccc
    35761 cgcaaagacg aataacgggt ataacgttgg ttttccagcc gattcagcag gcgagataga
    35821 atgtcgagac aataagaaaa taaaaccgag aaaacacagc agtgtcacta agatcgaaat
    35881 gaagacaata ctagcaaaat agatccataa ttgtgaaaat agcttttttt catctttaaa
    35941 taaccgcctt atagcctagt ccccgtactg tgatgatttg aaaatcttcc gtcttttcaa
    36001 aacgggtcct taaacgtttg atatgtacat cgatcgttcg ttcttctgta ttcgtatcta
    36061 atccccaaat atcatccatc aactgttggc gagtgaagat cttgttggga taagacagta
    36121 atttatacag aagcagaaat tctttttgag gcagaatcaa cgaatgctga tcttgtaaca
    36181 ctgtataagc atctagctct aattttgttt gtccgattat caagctactg ctgtaattga
    36241 tctttgaacg tcgaagcaat gcttccacac gtaggatcat ctcattgaca tcgatgggtt
    36301 ttgtcatata atcatctgct cctaatttga acccgcgttt cttatcctca aaatcggcct
    36361 ttgccgtaat cattagaaca ggtgtgtcaa atctggtgct acgtaatgag tctagcaagt
    36421 catagccatc cattttcggc atcatgatat ctgtaatgat taattctata cgtgtcgctt
    36481 ctagtttttt cagcgcatcc tctccattca atgcggtgat gactgaaaag cccgctcgtt
    36541 ccaacacaga acggtaaagc aactgtaatt gcggatcatc ttctactact aaaatacgat
    36601 tcattgatct ccctctgttt tttgaaaaat ttctagattt tttcttgtag ttcatcttca
    36661 gttaacattt atttcgtata ctacgatcat tgttctgata tacttattag tataccgaat
    36721 atttcaatac gggagaaggg tgattatgct tcaattaaaa gatataaaaa aatattataa
    36781 agtcggcgaa accaccacca aagcattgga tggtgtgtct gtcgctttca gacaaaaaga
    36841 attcgtagcg attttaggtc ctagtggttc tggtaaaacg actatgctga atgtgattgg
    36901 tggactagat aattatgatt ccggtgatat ggtaatcaac ggaaagtcta ctaaagactt
    36961 taaggacagt gactgggatg cctaccgaaa caactctatc ggtttcattt ttcaaagtta
    37021 caacttgatt ggtcatctag gcattattga aaacgttgaa cttgggatga cattaagtgg
    37081 tgtttctaaa gacgaaaaac gaaaacgggc agaagatgca ttgcaccgag tcggcttgac
    37141 aaatcatatg cataaaaagc caaatcagtt atctggtgga caaatgcagc gagtggccat
    37201 cgctcgagca ctagccaatg acccagatat ccttttgtgc gatgaaccaa ccggagcgtt
    37261 agatacagaa acaagtatcc agatcatgga attgatccaa gagttatcga aagaaaaatt
    37321 agtgatcatg gtcacacata atcctgaact ggcccatcaa tacgcagatc gaatcattga
    37381 attttctgat ggtaagatct taacagattc ccatcctcac atcgaacgac caaaagatga
    37441 tcaatttaac ttacgcagaa caaagatgag tttttggaca gcgttaaagc tttccttcaa
    37501 caatatccga acaaaaaaag gacgtacttt cttgacctcc tttgcttcca gtattgggat
    37561 catcggaatc gcgatcgttc tgtccctttc ttctggattc caaaaacaaa tcgataacac
    37621 gcaagctgaa acgatggcga aatttccgat tactatttct aaggtaacga ctaaccagac
    37681 tcgtgacgat gccggattgg gtgcaagcaa agcggattat ccagacagca aaacgattac
    37741 tgcaaaagtc agcgaagaag ataaagcaca gcacacgaat aagattgatc aaacatacgt
    37801 cgattacgta acagacatcg atcctaactt aagtaataat atcggattta cccgtactac
    37861 aggaatcaat cttttacgtg atgtaaatgg caaagtacag cctgtcagct tctcgaatca
    37921 aaatcctgat acggaatctc tttctttatc tagtacgatg tcagcaatga caggtgtggg
    37981 agtgtcaagt ttccctacac agcttgacac tagcaaagaa aactttttaa aagacaatta
    38041 ttctttactt gccggaagtt atccagcctc agcaacagat gtcgtgctga tcgtagacgg
    38101 caataataat actaatatca acgcattgaa gaatcttgga tttgatgtga aagaagacga
    38161 aaaacttgat tttgatgata tcgttggtac aacattcaaa ttagtaaaca acaatactta
    38221 ctatacaaaa cttccaactg ggaattttat ccctaataca gattatgatg cgatgtatca
    38281 gaatgcttct gatgaattga agatctcggg gatcctacgc gtgaaatctt cttctacaat
    38341 gaatctctta tctccaggaa tcgcttatag cgatcaatta acgactcaaa tcgtcaacga
    38401 aaacaaagaa tcagaaatcg tcaaagctca aagagacagt ggtgtaaatg tcttaactac
    38461 tgaaaaagtt gacgaaaatg ccaaacaaac attgcttagt tatttaggtg gagacagctt
    38521 gccttcaagt atcatgattt atccgaacaa ttttgaagac aaagaaaaga tcttggatta
    38581 tctagatgat tacaataaag gcaaatcaga tgaagataaa atcatttata ctgatttagc
    38641 tggaacgatg actgaactga ctggcggatt gatggatgct atcacttatg tccttattgc
    38701 ttttgcaggg atctctttag taacaagtat gatcatgatc agtatcatta cttatacatc
    38761 tgtgattgaa cggacaaaag aaatcggtgt actaaaagct ttaggtgcac gtaaaaaaga
    38821 catcactcgc gtatttgacg cagaaacgtg tattttaggt atttcttctg ggatattagg
    38881 tgtgtttatt gcatggctag ctacgttccc tatcaatagc atcttataca acatgacgga
    38941 tctaaaaaat gttgctcaac taaatcctgt ccatgcgatt attctagtga tcgtctctac
    39001 tatattgacg atgcttggcg gtcatcttcc tgcacgaatg gcagcgaaaa aagacgccgc
    39061 tatcgctttg cgtgcagaat aaggttgtga caaccacatt tagatttgag tcataagagc
    39121 gctgaaatag tagaaaacag caagaaacga ggaagtaata tgttccttgt ttcgcgctgt
    39181 tttttttaat tattttttat aattctggta tacttttctt tcctttgtac cacaactctt
    39241 ctggcgtata cctgtctttt ccatttatga atactgttcc atctgcttca atcgttactt
    39301 cagtcccaat ccacatatta cgagaaagtg cgagaagatt ttttcttttt cctcttcctt
    39361 tttttccaaa ttgcaaggca tcggttatct cttctgtcca atgagatttc acgtatttta
    39421 cttctccgtt ttcaaatgta actttcaaaa tattcgctat attttccact tttactgctt
    39481 taggaaatga ctcctccatt tttttcactt tccttcctcc aacactaagt atgcgctttc
    39541 ttttagtgat aagtatatca tagtttattc tattatcaaa aaaacgcatc cattccattg
    39601 tagaatactt taccgaacaa gttgaattgt tttcctcttt ttcacctttt actatagata
    39661 ggtaaagaca actaaggagg aatctctcat gacagatcaa ccgtatgatt taagaaaagt
    39721 catcgaagaa ctaaaaacac ttccaggtca atatcacgaa acagatattg aaatcgatcc
    39781 tgaagcagaa ttatctggtg tttaccgtta tatcggtgct ggcggtacag taaaacgtcc
    39841 gactcaagaa ggaccaacta tgactttcaa caatatcaaa ggtttccccg gcacacgtgt
    39901 gaacatcgga acaatggcta gtcgtaaacg tgtaggtgaa attttgcacc atgattatcg
    39961 aacacttggt cgtttcttga aagacgctgt agaaaatcca atcaaacctg tgaaagtaga
    40021 caaagcagac gctccagcac aagaagtcgt tcatctagct accgatgcag acttcgatat
    40081 tcgcaacatt ttgcctgcac ctacaaatac tcctgaagat gctggtccat acatcacaat
    40141 gggtgtagtc ttaggatcag atccagaaaa aacaatgagc gatgtaacga tccatcgtat
    40201 ggttctagaa gataaagata cgatcggtat gtacatcatg cctggcggac gccacattgg
    40261 tcacttccaa aaacaattcg aagccttgaa taaaccaatg cctatcacta tcaatattgg
    40321 tttagatcca gcaattgcca ttggcacgac tttcgaacca cctaccactc cacttggcta
    40381 caatgaacta tggtgtgcag gagctttgcg taacgaacct attcaaatcg ttgatggtat
    40441 agcagtcgat gaagtgggta ttgctcgtgc tgaattcatc attgaagcag aaatcatgcc
    40501 aaatgaaaca atgcaagaag atatcaacac gaactcagga aaagcaatgc cagaatttcc
    40561 tggctatact ggggatgcta atccagctgt acaagtagtg aaagtcaaag cagttactca
    40621 ccgaaaagac aatccaatca tgcaaacaac gattggtccc agtgaagaac acgtatctat
    40681 ggccggtatt cctacagaag caagtattct tgccttggtc gataaagcta ttccaggaaa
    40741 agtagtaaat gtctacaatc ctcctgcagg tggtggtaaa ttgatgacca tcatgcaaat
    40801 ccgtaaagaa aacgaagcgg atgaaggtat tcagcgtcaa gcagctattc ttgctttatc
    40861 agccttcaaa gaattgaaga cagtcatttt agtcgatgaa gatgttgata ttttcgacat
    40921 gaacgatgta gtttggactt tcaacactcg tttccaagga gataaagata tcatcgtgat
    40981 cccaggaatg aggaaccatc cattggaccc atcagaatta ccagaatata atcctggctc
    41041 tattcgtttc cgtgggataa gtgcaaaaac tatctttgac ggtacagttc ctttcgatat
    41101 gaaagaacat tttgtacgtg caaaattcca agaagtagaa gattgggaaa aatatttgaa
    41161 ataatgaatg atctgcaact gttttgacat cataaaaagg agtgcggaaa atggaacaat
    41221 tggcacaacg gaagaaaaaa atcgtgattg gtgtaagtgg cgcttcaggc accatttatg
    41281 ccatcaacct agtgaagaaa ctaaaagaat atccgatgat agaaacacac ggaatcatca
    41341 gtccatgggc aaaacagaac ttaaagctgg aatccgactt atcactaact gaattcgaaa
    41401 gcctgtttga ttaccattat tcaaacaaag atttaggtgc tgctgtcgcc agcggatcat
    41461 ttttgaccga tggaatgatc atcgtaccgg caagtatgaa aacagtcgca ggtatctctg
    41521 ttggtttagg cgacaacctg attgcacgtg ctgctgatgt aacaatgaag gagcaacgaa
    41581 agttagtcat tgttcctcgt gaaacgccat taaatgccat tcacttggaa aacttgacta
    41641 aattagcacg actaggtgtg caaatcatac caccaattcc tgctttttat aatcagcctc
    41701 aaacgatcca agatcttgtt aatcaccata caatgaaact tttggatcaa tttgaaattg
    41761 taaatgagca ttcaaaacgt tggcagggtg attaatatgg aaaaagaaac gatagttgaa
    41821 gtaaccaaac taggtttcac gatccaagct gctgttaaag taatcggtaa agacttattg
    41881 atcactttaa caggcggaga tacaccacat atcggaacag tcactacatt ttctcgtgat
    41941 acagagatgc aaactgtacg tttccctagc cacgatggtc ggttccataa agacgatgtc
    42001 ttagccgaaa aaatagctgt gatcatcaaa actgccgttc cgggaagttg tacgatcact
    42061 tccggtatcc atgttgacca tatctccaat gcacaaatca acgcctcttt tccgatgtca
    42121 gaagagttag gttacaagat tttagattgg ttggaaaaaa tagatttcca tatcaaagaa
    42181 ccagtttatt ataaagatgg tgaaaaacca ttatgaaaga agaggttgtg aaatcagcgt
    42241 tatgacctga gtatgagaga aaaaaatgga aaaatagctt ctcatatttt gccgtttttg
    42301 agctaatacc gagggacagc tgattgaacg ccgtttatca agaggttgtg ataaagccgc
    42361 ttcgtttcga gtagtaagaa aaattttgta aacatgggtt tccacatttt tgcataaatt
    42421 tgacttacta ccgagaaact ggcttttgaa caccgtttat caagaggttg tgacacgtct
    42481 tttgtcacaa cctcttttat ttttgcttca ctgtaactgc tacaatatca ccgatttctt
    42541 ttccgatgtt tttacgaata tcttttcgta gtccaaggat gtagcagact gaaccatctg
    42601 gatttttcac gcccatattc acgatactgc cttcatatgg cacattgtca aaagttgctt
    42661 ggactttaac tcttccagca ccgaattctt ttcttatatc atacggaaaa ataatatatg
    42721 ccccgccctt gccgacttcg gaagcataga tttttgattc aaaagaataa atttccctca
    42781 ttttatttct cctatttttt acatctagta tagcatagca aaaaaaagaa ggaactgtga
    42841 caaaagtctt gtcgcagttc cttttttcga ataaacggtg ttctcctgcc aactcctcgg
    42901 tcacaagtca caatagtcaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac
    42961 tcagagttaa acggcaggct cacaacctct tccttaaacg gtgttcaaaa gcttgcatct
    43021 acggcactaa gcccaatcag atgaaaaata tggagtgcta tttttatctg attgttctta
    43081 ctgcttgatg cagagcagct ttttcacaac ctcctacatg gtgttcaaaa tattattcgc
    43141 cttttgcttc tttcactaat tttgcgaatg cttgttgttg gaattcagca gctaaggctt
    43201 tcgtacgttg ttcttgctct gctttgctca tttttggatt ttcagtcatt gcttttacca
    43261 taccgtcttg aacataagct tttgcttcac tttccatcgt actttgctgt ttcttcacta
    43321 attcagctaa cttggctgct tggtccgtac ttaattctac ccatgtctct gggattttga
    43381 atgtattcac tcggctgtca tattcgtgat tgttatcagc aatattgaac cagaatttat
    43441 acatgtcgtt tttgtattcc caataagtag ttttcgtcac tttttcagca tcgccgtctg
    43501 tcttttctac tttatttgtt tcgttatccg taccagttgc ttttggtttt tcttgttttt
    43561 catttgttcg gtaaagatac actttttctt ttccattccc taaagcttta tacaaaagca
    43621 tatctgctcc ttgggaatca gcactggaaa caattttttc tgtttttgtt tccgttactt
    43681 tttccattcc aaagtgattg gacaaattag cgacgatcaa acttagtgaa gcgacgaaga
    43741 ttagtccgaa aatcaaagat aaaaaggttt gccatgtttt tttagcgaac acgttcgtta
    43801 aagcaaaagc aagcaagctg acaatcaaaa ctccaataat catgcattat cccccgcttt
    43861 cattagaaca ggttcttttt tcttcaataa aaaggcaata ataaagccaa gaatcccaaa
    43921 gatgatcgct acgataaacg cggcatggta acctgacagt gtggcattga ttgcctgatc
    43981 tttataagct aatggcgtat tatccaatac agatttgctt gggatatttg cttttgtcac
    44041 attcgtcaac acactgatca aaatagctgt accaatcgag cttgcgattt gacggaatgt
    44101 attatttacc gctgtcccat gactgatcaa gttatttggc aatgcattca taccagaagt
    44161 cgttaccggc atcatgacca tggagatccc aaacatacgg atcgcataaa ggatcacgat
    44221 atataaaact ggagtcgttt ttgtcaagaa agcaaatggt gcagttgccg ctgtcaaaat
    44281 gaacatccct gtgatggcta aacgtttcgc gccgtattta tcgaaaatcg cacccgtcac
    44341 aggcatcata gcccccatga tcaatgcacc cggcaacaac atcaagccag aatggaatgc
    44401 tgattctcca cgaatatttt gaatatacaa tggtacgacc atttctgccc ctaccatcgc
    44461 catatttgtt acaccagcta aaacagctgc aatcgtgaat gtagatgatt tgaatacacg
    44521 aagttccaag aatggatgtt ccaaatgtaa ttgacgccat gagaacaagg cgatgaaaat
    44581 cacaccaaca actaagaaac caattacttt caagcttccc cagccgtcat ctcctacact
    44641 agaaaatccg taaagtaaac taccgaatcc aatcgttgat aaaatagcgg ataaaatatc
    44701 aatgctagga tttgacaatg gaataacact tttcattagg aagaatgcta aaaccaagac
    44761 aagaacaacg attggcagta ccataccaaa caagtcacgc caatggtagt tatcaatgat
    44821 ataaccagat aatgtagggc ctaaagcagg agcaagtcca ataacgatcc ctgacatccc
    44881 cattgctgat cctctttttt ctggcgggaa aatagatagc atgatatttt gcaataatgg
    44941 catcgatact cccacaccgc aagcttggat caaacgaccg attagtaatg tactaaaatt
    45001 aggtgctgta aagcaagtaa tcgttccgat caaaaagatc gtcatggcag aaagatataa
    45061 atttcttgaa ctgaatttat tgatcagcca tgctgttatt gggatcatga tcccgttgac
    45121 tagcaagaaa ccagtcgtta accattgcac atcagacgct gaaatatcga aagctttcat
    45181 caatgtagga aaagctgtcg tcaataaggt ctgattcaaa acggtacaaa atgtaccaat
    45241 taataagaca acaactaaaa gtgtccgatt atatggtttt ccgtaaatat ctacgggttg
    45301 gttgttactc atatttctcc ttctttctga attttttctg aaactactag aatactttag
    45361 tcctaaattc gctatttgtc aacctacttg acacataagt taatacggat ataattaagg
    45421 gaagcaagag gacaaaagga gtgacaaaaa tggttggggc ttcccttaaa cgtttgttgg
    45481 aaaatgatca attggttgtt ggcatcagtg aattaagtga tatcgtaggt gtgtctccac
    45541 gtcaattaag gtattgggaa caaaagggat tcattcgttc gatcgaagct gatgcgaatt
    45601 gtcctagaaa atatcgttta cctacagtca taaaagtaga actgatcaaa aaatatttag
    45661 atgacggatt tactttgtca aaagctgcag aaaaggcaga aatccgccaa aagaaaatgc
    45721 accatgtccg taaagttttt tcaaaagcaa tcaaagatat cgaattgatc gacgaacgct
    45781 atacgatttt acaaatcggt aacttcaaag aagagaatgc taagatgtac atcattcatg
    45841 acaacgagac agacgaatta acttacaagc tccttcctgt cgatgaaaaa gctgactacg
    45901 aacagttgtt aaaagaactc taaacgccac taatctccta agtaccaagc tttttaataa
    45961 gtagatgcta cataaatact tgaaaaaagg cctgtttgaa cataagaata tccgaacggt
    46021 gatctgcgtt tctttcatat agaaaggaat tctccatcac tgttcggata tttttttatt
    46081 tttttcggtt actttcttct aatacatctg ttagccaatt tgcatattct gtttcccgat
    46141 tgatcgcatg cgctaaaatc aggtagtgtc cgtaattttc atgtttttct ttttcagtag
    46201 gaaagatcgt tttttttcgc tctaatagat gaagtcgttt ttcttcatga aggtcacgct
    46261 gctgctgaat aatttctact aatgccggat cattcacgtc tttgacgaaa tacaatttca
    46321 gaacaaattc gtctcgatta accggtagtt cattggtggg tgtatgaatc cactctgcca
    46381 attgttcctt cccttcttct gttaattcat aaactttctt ttccaatttt tcacctgtga
    46441 tctctacttg atgctgaatg attccttgct cttccatttt tgcaagttca ggataaatct
    46501 gactatgttt tgcttgccaa aattctccta tttcattttt gaacgattga ttcaattcat
    46561 atcctgtcat cttcttctta cttaataacc ctaacaaaat atatcgaagt gtattttttc
    46621 ttgccatcag ttttccctcc ttgacaaatc tttaaatcca ttctatcata aatgagtaat
    46681 cgaaaacatg tagtttatta catgttttaa tttataagga gatgcttaaa catgaaagaa
    46741 tttaaagaat tggaagattt tattggaact cactttatct atacgtatga caatggttgg
    46801 gaatatgaat ggtatgccaa aaatgaccat actgttgatt accgtatcca tggcggaatg
    46861 gtcaaaggcc gttgggtaaa agaccaagaa gctgatatcg ttaaattgac ggacggcgta
    46921 ttcaaaatca cttggacaga gccaacaggg acagacgtgg cattggactt tatgccaaat
    46981 gaaaagaaac ttcacggaat gatctttttc cctaaatggg ttgaagaaca tccagaaatc
    47041 actgttactt tccaaaacga acatatcgca gaaatggaag cagctcgcga aaaatatgaa
    47101 acttatccaa aattacttgt tcctgaattt gctacgatca cttatatggg tgatgcaggt
    47161 atagacaatg aagatgttat tagcgaagaa ccatacgatg gaatgccaga tgatatccgt
    47221 agcggaaaat acttcgatga aaattataaa cgagtaaaat aaggttgtga aagagaggtt
    47281 gtgaaatcag cgttctgacc tgagtattag agaaagaaac ggaaaaatag ttcctcatgt
    47341 tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt atcaagaggt
    47401 tgtgaaatca gcgttatgct ttgagtaata agaacgctag ttagaaaata gcttttcata
    47461 ttttctaggc tagtgggctt attaccgaga agcagccgtt tgaacaccgt ttatcaagag
    47521 gttgtgaaat cagcgttttg acctgagtat tagagaaaca aatggaaaaa tagttcctca
    47581 tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcaaag
    47641 attgtaacaa ctgcatattc acacagttaa attagtgact tcaaaatgaa ataatgctcc
    47701 cagcaaaaaa ttaggctggg agcattattt cgttattctg ttcatcaaaa atcagcgttt
    47761 tgattccatt tcatcacaag ttcctcctca tttactgctt catctttttg ctgtgtttga
    47821 attgtaaaac cttgtgaaag atagaaatga atagctgatt gatttttttg atagactgtc
    47881 aaagtcagtg ttttattggt ttcttttatt tttttcatta gttgcgtacc gattctttgt
    47941 tgccggtatt cccttttcac aaagattcct gcgataaact gatcaatcac cccagcaaaa
    48001 ccaataattt cttgatctat ttctgctaag taaatcactg catctggtaa agctttccta
    48061 acagattcta cttgattctc ccaataagtt gatggaatga attcatgaga atccaagtta
    48121 gcatgcagcc aaatcgatag aacagattcc agttctttgg cagacaattt tgttgttgtg
    48181 cggatcattt tttctctcct tacttattca ttgattttaa aataaaaacc atttttttga
    48241 agctctttta caagttttcg cccctcaaaa aagtatatct tctcttgttt tacttttcta
    48301 ataccaaaag aaacagcaac tttaggatca tcagctgaaa cgattcgata agttccacca
    48361 ggcaattgat ccagcttgac tttcccatga tggtcgcttt gtactgaaaa cggcacacca
    48421 tttctagtaa gtgttttttt cttatgtttc aaaatgaatg aactaccatt taccggtttc
    48481 cctgacgaat cactaataat aaatgaaaaa tcaagcactt ttttacttaa tctatactga
    48541 tagattagtc gtccgataaa cactaacagt atgactacag ctattatcgt tcctgcaaga
    48601 ataaaccatt gccgcatggt tctttcctga tttccttttc cttcttcttt ttttaccgat
    48661 tcgttataag gaatccgatg tcctgtcact agcaaacgat gcgtgttgat catatacggc
    48721 gtacatgtaa gcaaggtaac taagtcttct ccttctgaaa tcgttagaac agacgtgtca
    48781 tctggcagta ccacttgtat atttttaact tgataagcca atttctcccc caaaacagtc
    48841 aaaataaacg tgtctccgtt ttctaaacga tctaattcgg taaataaccg tcgattaggt
    48901 aaaccgctgt gagcagagat cactgaatgc gtcgaatttc caccaagcgg gaaagaggtt
    48961 ccttgcaaga cgcctgctcc attttcaagg atcctaccat tgagccgatc ataaagtgaa
    49021 atatttaatt ggatcttagg tatattgata cttccgatca gccattcctc taattggctg
    49081 gatgttgtac tttcatttct gttcttttca ttgaaaggat cagtaccagg attgaatcct
    49141 tggtcagtta atcgttggtt tttctcctcc attttctttc gctgcacttc aatttcagct
    49201 gcggtccgtt tttgtacttg ttcgatacgt ttttgatcaa taaaactatt taaagaatcc
    49261 acataaaatg gatataaagc gatcagtaac cctacgacga tacatattaa taaaaacaaa
    49321 cgaaacaaaa gtcgacgaga ctgtttttgc ttactctgct tcacatgtgt cctcccttgc
    49381 aagttttttt gttctttctt ttattatatg ggttcaggaa aaaaatagaa aatgaaagcc
    49441 gatgaggttg tgaaaaagct gtcctgcatc aagtaataag tacagcccaa aaaaatagct
    49501 actcatattt tttgttgggt tgggcttatt accgcagatg caagcttttg aacaccgttt
    49561 aaggaagagg ttgtgaaaac tgcgttatgc tttgagtaat aagagcgcta gtcagaaaat
    49621 agcttctcat attttctagg ctagtgagct tattaccgag aagcagctga ttgaacaccg
    49681 tttaccgaga ggtcatgaaa aagctacttt gtttcgagta ttaagaaaaa ttctgtttca
    49741 aaacaaggag atgcaacaaa acttatgtcg catcctctct ccttagaact ttcacttatg
    49801 tttttcttgt aaaattcata aatattttca tgaattttct aatttcttgc ctacctctcg
    49861 cataggatat atgatgaatt tatctaaagt ttgcaaagga gttcgacaat tgaaaaaata
    49921 tggcccattt ttagcaattt ttttcattgc tttggcattt ctcatgcctc atttattcac
    49981 acataaacta attttgggtg ctgatgcccc attccattac aatcggttct atgatgccgc
    50041 gcaacaaatt aaatatgggg atttccatta ttttccttcc ctttatggat tccagcaaag
    50101 tggcaggatc gtgaatgccg tttacggccc atttttcgct tatttacaag gattgatcgt
    50161 gttgcttgca ggtagctggt ttcattatca gattctttca aatttcattc tttatcttat
    50221 ttcaggattt tctatgctga ttttactgag atatgtcaat gttcgaaatt gggtaagtgt
    50281 accagtagcc gttttattca tgagtacgta ttctattcaa tactggtcta tcaaccaagg
    50341 attcactagc tgggggactt gtttttatcc tttatgtatg atccctattg ctgatttcgt
    50401 ccttcataaa aagttccctg ttatcaaagt cgctgtctca gtgggactta tgacacagat
    50461 ccatttactg tcttcgatca tgttagcttt gatgtatatc cctttttatc tttattactt
    50521 ttttaaccag actgaaaaag gaaaagcact gcttcaactg attaagtcgg tcttgcttta
    50581 ttttgttctg acagcaaata tttgggcagg attagctatt gtttacagtg ggaataagat
    50641 tcttcctccc tttgttaaca gagaaatgag tcaaaaggcg atagatctgg atggcagtta
    50701 ttggctccat taccctaagg cctttcctat attgatatta tcaggttttt tgctttttat
    50761 tttctttcaa caaaagaaca ccttattcca aaaaatcgtg ttgtacacaa gtcttgtttt
    50821 cttttttctt tctactagct tgattccatg gacgacattg atcgaaaaaa atgtgccttt
    50881 tattagtttg atccagtttc catttcgatt tttcgtgcca tttacgctat tgcttctact
    50941 ttaccttgcc ctttcactga accagtggtc tgaacaaaag tggttccgtc ttttaagtat
    51001 atttggcatt cttatttttt cagcacagac gatccagaac ttataccagc atctggaaaa
    51061 gtgggaaaat gaaacatttg tcagtcgaca tacttatcta ttcgatacgc cggaagaagc
    51121 aaggaaaaca ttcttcagca aagatatgtc taccagcttg ttcgttttcc aaaaaaccac
    51181 acctgactat ctgccaatct ataaggagac aaaagacaat aaatacgacc ggtataacga
    51241 agaaatacta gaaaacgaaa caacttttat aaaaacacat ccaggcggtc ttttgacaat
    51301 caagtggaaa aacactgggg acaaaactgt tcatatccct gttatcgtct acgatcggac
    51361 tgtgctacag caaaatggaa aaacattgac tgattatgaa gtaacagata tcgggacccc
    51421 aatcgtgaag cagcaaaaag gaatcaacga gctcacgctt cattatcaaa cgccaatcta
    51481 tttctatttc attctttctc ttactttgat tggctggttt accttgctat gtttatttat
    51541 ttatcataga tacaagcttt tacgtgctta ggcaaaaggg ataaatacat ccgatcaaaa
    51601 aactctccaa acggtataat cgtttggaga gttttatttt aatcagtttt tttatgttat
    51661 atgttatatt ttttattcgt tcacatctaa ttcgatttcc accccgtagt gatcactgat
    51721 gattggacta tcgatcccat caaaaatccg ttgatatttt ttaacctgca ttccttcagg
    51781 gacgaaaata aaatctatac gcaatttttc tgtattttgt tcccaaccat ctattttctt
    51841 ttccacagta gctgcttttc ctttttcatt cgcgacaaca tacgaatctt gaagaagaag
    51901 gcaactttct cttacagtat aataaccttg agagtctact ggattattga aatcacctag
    51961 gaaaaaaagt ggtacgcgag tttccaaaaa gtagttttcc aagtttttcc actcaaaata
    52021 aaagccactt tttttatctg tccaccaaga aaagtggcag ctcgagacaa aaatgtcctg
    52081 atcatctatc tttgttttcc caatcaagat cttgcgtgtc tgataatctt ctttcttttg
    52141 aaattgggag acggtaagga cctgactttc caatgttttt ttcgtaagca gtgcatttcc
    52201 ttcttcatat ttatcataac cgatgtggct catttcccaa ctccaaaaat actcttttcc
    52261 gtgttccttt aaaagctgaa ctaaacggta ggcaaaatta tctttacgga taggtacttg
    52321 attctttacc ggacagaact tcattagctt cgcaggttct acttcttcag attccagtaa
    52381 ttggttgatt tcttgtaaag caatcacttc ataatcttct aacaggatct tatctgctaa
    52441 ttcttgcaat tttttctctg gttctggctc aagccagcta tgcgtgttga gtgtcatgaa
    52501 tttcattggt tttctccttg tcttcatttc gaaacaaaag ggttgtgaca aaatttttgt
    52561 cacaacctct ccagcaaata gtgttctcct gctaactcct cagtaataag ctcaatggac
    52621 caggagctat tttttgtttg gctgttttta tcacttggtg cagggcagct ttttcacgac
    52681 ctctccacta aacagtattc aaactgctgc ttctcggtaa taagcccaac caaacgaaaa
    52741 ataggaggag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    52801 aacctctcct tacattttaa tggagccgat cactttacca gctgtcactg tacctaattg
    52861 cttgatcact acttcttcta ctttatcgtt tgtaaatacg atcatgatcg ttggatcttt
    52921 ttcttcgttt tggatcacat caagatccat ctcagctaat ttcgttcctg ttcctacttc
    52981 ttggccttct ttgacagcaa tctcaaaagc tggctgattc atttggactg tatcgattcc
    53041 catatggatc aaaacttcga taccatcttt cgtttccagc ccaattgcat gtttgctagg
    53101 gaaaatactt gtcacttttc cagcgatcgg cgcaacaact tctcttgatg ttggaataac
    53161 agcaaagccg tcacccatca ttttttgtga gaataccgga tcttttactt cagataaagg
    53221 aatcacttct ccagaagctg cggaaactag ttcttgttct atacctaagt attgcttact
    53281 acctgcttgt ccatttcctg tagaaaccat ttctggctct ggaatctctg ctcctgattg
    53341 cagcagatct tcgatatctg atttcaagac atctgctttt ggtccataaa ctgcttggac
    53401 tccattgtct ttgacgatca atcccatcgc ccctgctcgt ttccaggctt cttcagtccc
    53461 tactttttct cgatcattta cacttacgcg taaccgagtc atacaagcat cgacatcttt
    53521 gatattttct tttcctccca gcaaatgaat gatctggacc acttgctgat ctgctgttcc
    53581 tgttccgctg ttagatacaa cggtttcttc actgtcattg tcgtaatttc cgttacgtcc
    53641 cggtgtagca aagttgaatt ttttgatcaa gaaatttgct aagaaacacg taacaacacc
    53701 aaaagcgatc acacacaaca cgaagttgat aagatctcca cctagacccg ctttgatagc
    53761 taacggtgtc cgagtcagca attcgatatt cccaaatgaa tggacccgca atggcaagat
    53821 atctgccatc gcaaatgcag ccccttggat cactgaataa atgacatata atggcacagc
    53881 agcaaacata aacatgaact ccaatggttc tgttacacct gttagaaaga cagccaacgc
    53941 tgctgaaaaa tacattgatt tatatcttgc tttcttatca ggatcgacat tacgatacat
    54001 cgcaaaagcc attcccatca agatacctga agaaccaatc atttgtccta ctttgaaacg
    54061 agccggcgtc cagttatgca acacaaactc atattgcgac atatcccctg cacctttaag
    54121 attcactaaa tcagtggccc aagccaacca taacggatct tgcccaaata cttgtgtccc
    54181 cgcctgtgcg cctgataaaa tctcatacga tccgccaagc tgtgtatagt tgataggaat
    54241 cgtcagcata tgatgcaatc caaatggcaa tagtaaacgt tctaatgttc caaataaaaa
    54301 cggtgctaga atcggtgcgc tttcttgtga ttctgcaatc cataaaccaa aattattgat
    54361 tccagcctgt acgtttggcc acaccacagc aagaatcaat gccacgattg ttgaccataa
    54421 gataacaaca aacggaacaa agcgctttcc attgaaaaat gacaaagcat ccggcaattt
    54481 tctataatta tagtacttat tataagccat tgccccgaca aatccagcga tgatccctac
    54541 gaatactccc atattcaaag ctggtgcttc caatacactt gtaaaaaatc ctttaaccat
    54601 gatcttcgta ccgaacagag tgtgggtaaa tgcatcttca ttcgcaagca tgtcacttgt
    54661 gacaccaaag atagccccag tgatccgatt gattaaaata aatgagatcc cagctgcaaa
    54721 tgctccgcct gctctttctt ttgcccaact tcctcctatt gctaaagcaa ataaaatatg
    54781 aagattacca ataattgccc aaccaatatt ttcaattact ccgcccgttg tgacaagcgg
    54841 cgtccaatca ggattgatca agggaattga tttccctata ctgatcatca acccagctgc
    54901 cggcataact gctaccacaa ccattaaagc ctttccaaat ttttgccaaa actcaaaact
    54961 aaatatcttt ttcattccgt ttagctcctt tacgcaatcg gtttcgttaa acgtattata
    55021 gcgtgcgttt tcttttttgt aaatgcttta ttttttattt ttctgtctat accattctat
    55081 aaaacctata atacgtgtgt tcccttctat aattttctcc taaaaattta tttttttaag
    55141 cttttttcta tgaaatattt tcttttgtct attgacttgt gcaatcggtt gcgtttatta
    55201 tgaacgtata aaacagctca ttaggaggag aaaaatgaaa cagatcaaac gactatttgc
    55261 tattgatcca tggaaaatca gcacgacaca attagataaa aaaaatcttc gactgcaaga
    55321 atctttaaca agtattggaa acggttatat ggggatgcgc gggaactttg aagaaactta
    55381 ctcaggggac caccatcaag gtacttattt ggcgggtgtc tggtatcccg ataagacacg
    55441 tgtcggctgg tggaaaaatg gttatccaga atactttgga aaagtgatca acgccttgaa
    55501 ctttatcgcg atagatattt cgatcaacga tcagctgatc gacttggctt ctttggaacc
    55561 agaagacttt tactgggaat tagatatgaa aaatggtgta ttatctcgta cattcacaat
    55621 cactacttct actaataaag tacgcatttc cttcgaacgt tttctgagca ttgtcaaaaa
    55681 agaatcagct tatatctatt taactataga aatgcttgaa ggtagcggaa cagtcaaact
    55741 cgtatcaaaa ttggatggaa atgttcaaaa cgaagacagc aactatgatg aacatttttg
    55801 gaatgagatt gatcgtggaa ctatagaaga aatcggctat ttgacgacta aaactattcc
    55861 aaacaacttt gggattgaac aatttactgt tactgcagca atgcgccatt tcgttaatgg
    55921 aaaatcagtc atccctgact atcacgaaga tactttagca ttacatggtt gcttgtctgc
    55981 agaattatct caaggtgaga catggtcagt ggaaaaagaa attatcgttc ttactagtag
    56041 agatattcca gaagcgcagc aagcagaaaa agctgtctca gaattaacga accagacaaa
    56101 caattatatc caagcaaaag aagagcaaac agcggcttgg gcagaacgat ggaagcttgc
    56161 cgatgtggtc atcgaaggag acgaagaagc acaacaaggt attcgattca atttgttcca
    56221 actattctct acttactacg gcgaagatga acgattgaat atcggcccca aaggattcac
    56281 aggagaaaaa tacggcggag ccacttactg ggatacagaa gcgtatgctg tccctcttta
    56341 tcttgcttta gccaaacctg aagtaactaa gaatctgctg aaatatcggc ataatcaact
    56401 gccgcaagca atccacaacg ctcagcaaca aggattaaaa ggcgcgcttt atcctatggt
    56461 caccttcact ggtgtcgaat gccataacga gtgggaaatc acattcgagg agattcaccg
    56521 aaatggcgcg attgcctatg caatctacaa ttataccaat tacaccggcg atacggaata
    56581 tttgaaaaat gaaggactgg aagttttagc agaaatcgca cgcttctggg cagaccgtgt
    56641 ccatttttca aaacgagcaa ataaatacat gatccacggt gtgaccggcc caaacgaata
    56701 tgagaacaat atcaataaca attggtatac aaatacgatc gcagcttggg tcttgcgtta
    56761 cacgcgagaa aattatttaa cttatcaaaa agaagctgct atcactatca gcgaagaaga
    56821 actagccaaa tggaaagata tcgcagagaa catgtacttc ccagttgacg aagaattagg
    56881 aatctttgtc cagcatgaca cattcttaga taaagatctc atgcctgtat ccgatattcc
    56941 cacaagcgat cttcctctga atcagcattg gtcttgggac aaaatcctta gatcttgctt
    57001 catcaagcag gcagatgtac tacaggggat ttatttcttc aatgatactt ttacaaaaga
    57061 agaaaagcaa aaaaactttg atttctatga accaatgact gtccatgagt cttccctttc
    57121 gccaagtatc cattcgattt tagcagcaga attaggaatg gaaagcaaag ctgtagaaat
    57181 gtaccagcga acagctcgct tggatttgga taattacaat aacgacacag aagacggctt
    57241 gcacatcact tccatgactg gtagctggtt gtcgattgtc caaggatttg ctcaaatgaa
    57301 aactgcccat gatcagttga gattcgcacc atttctgcca aaaacttgga aagcttattc
    57361 tttccatatc aattatcgcg gacgtttatt gttcatctca gtcacagatg aacaagttac
    57421 tttcgagtta ttagaaggtt ctcctctaga aatttacgtc tatgaacagt cttattcttt
    57481 agcggatcgt ctagaaataa cgctaagaaa ggaggaagca aatgtttaaa ggggtattgt
    57541 ttgatttaga cggtgtcatt accgatactg ctgaattcca ttatcgtgct tggcgaaagc
    57601 ttggtcagga aatcggcatt acgatcgacc acacattcaa cgaacagcta aaaggcgtca
    57661 gccgagaaga ctcactctct ttgcttttag cttatggcgg aaaagaacac tctttttcaa
    57721 aggaagaatt tgcagaatta gcgaaaagaa aaaacgatta ttatttagag atgatccaga
    57781 cgatcgaacc aaaagatgtc ttccccgggg tcgtgccttt actggattct ttaaaagaag
    57841 aaaaaatcaa gattgctctt gcttctgcaa gtaaaaacgg gccattccta cttgaaaaaa
    57901 tgggtcttac accttacttt gatgctattg ccgacccggc agaagcaaca aacggaaaac
    57961 ctgcgccaga tatcttcctc cttgcagcaa aagctgtcgg actaaaagca gaagaatgta
    58021 tcgggatcga agacgcgcaa gccggtatcc aagccatcct ctcaagtggc gctcaaccaa
    58081 ttggcgtggg acgtgctgaa gaccttggag aggagatccc gcttgtccct gatacgactt
    58141 ttcttacgat cgattacttg aaagaaaagt ggcatgacca tggataatcc agagatctcc
    58201 gtcgtaccag ttccagagac tgaaataaag aaaatcacat tgaagaacga ctacttagaa
    58261 gtcgttcttc tcaattacgg tgcccgcctg caccaaatct tcgcaccaga caaagaagga
    58321 aaatcagaaa atgttttatt gtcttacgat cgtttcgaag atgttctaac agacaaaagc
    58381 ttttttggag caactgtcgg acctgtagcc ggaaggatcc gggatgcttc ttggggtgag
    58441 catagattgg agaaaaactg cggatcacac catatccacg gtggcacgaa aggctggtct
    58501 ttccaatttt gggaagtaga agttttcaaa actccatcat caataggtgt ggtcttttac
    58561 ttaaaagatg aattttctac ctatccagga ccaatcactg ctacgatcac ttaccgttta
    58621 acaaaaaacg aattagagat gacaacctct gctagtagcc ttgttgaaac catctgcaat
    58681 ccgaccaacc atgcctactt caacctttca ggaaatggaa caagagacat ctatgaacat
    58741 cagttaacgg tctttttaga tggtgtgctt gaattagatc aagaaaaact gcctactgga
    58801 aattggacaa aaaaagaaaa actgccaatc gactttagaa aatccccaac actgcaagag
    58861 atccttgcat gttatccaga tggactagat gatgtatttc ttttgcatca tcctaggctt
    58921 tccaaaactt ccttacaatt attcgagaag cattctggtc gccaaatgac tatagcaact
    58981 tctaataaaa gtatggtatt attttctacg actggattcg aagctgattt ctctgtcaat
    59041 ggaaaacaga tgcattcaaa ctacggatta gcaatcgaac cgcaagaaat ccctgatatc
    59101 gttcatttcc ccaaatttgg atcgatcaac ctccatcctg gtcaagaacg gatcagtcag
    59161 acgatttatc ggttctctgc acaataacaa gagaaaggtg tttttatcat ggctattacc
    59221 gtaaaagatg tggcgaaaaa agccggtgtt gccacctcga ccgtctctcg tgtcatcaac
    59281 gatcatccta gtatttccga gacaacaaag aagaaagtcc gaaaaattat ggatgaacta
    59341 ggttatgttc ctaacatgac tgcccgcaat ctaggaaaac gcatttccag tgccatcggc
    59401 gtaatcttac cccctcttga ttcaaaagag cggatcggta acccatttta tcttgagatc
    59461 atggaagcaa tcaacgaaga agcgcggaat tatgatatga ctacagcaat cgctactgct
    59521 aaaaattttg aattgctgtt agagaacgtc cagcgtatgc atttacaaaa acaagtagat
    59581 ggttttattc tagtgtattc agatagtcaa gatactgtca ttgattattt ataccaaaac
    59641 cacatccctt tcactttgat cggacagcct tatcatcatg agaacgaaat cgtttatgta
    59701 gataatgata accagcttct aggtaaacaa gcaacagaat ttttgatcca gaatggacat
    59761 aagaatatcc tgtttgtgac aaacacgact catgaaagtt tgtatttcga acgatatttc
    59821 ggttatcaaa aagcaatgat gatggctggt cttcctgcgt tatccagtgt agtactagag
    59881 catccggagg attatctgtc ctttgagaaa acgttgaaag aaaacgaagc cacagcattg
    59941 gtggtcattg acgatatctt cgcccttcgc acgatgcagt tggcgcaaat gtatggctat
    60001 gtcattccag agacactctc cgtcattagc ttcaacaact cgatcttctc aacattaaca
    60061 cacccttacc ttacaagtat cgatatcgat atctccgaat taggacgtgt cgctatgcaa
    60121 aagctgcgcg aattgatcca tgaagaattg agcagtggcg tacgtttggt catccctcat
    60181 cgcttgatca aaagagagac agtcgttcct ttagaaaacc catactaaat agctgactca
    60241 ctaaatacgc tgaccattta tcctatggtc agctttttat gtaaaagaag atactacttc
    60301 taacacattg tttatctgta aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc
    60361 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    60421 ttcacaaaaa tttaaaaagc aattttcttg agtttcttct tatttcttgg agttaaacgc
    60481 ttctgtccca gcctcttttt tccttccttc aattttttac acgtcgacga ttcaataaaa
    60541 aaccgcctaa tattgtaaag acaggtgcaa gaatcgagaa aaaggcgaat ggcaagtaat
    60601 gcaacgcgct aactttcatc gttcccataa tgaatgtgcc gctgactccc cacggtacga
    60661 gagaattgac ggtcgcacct gaatccgcta gtgttcgtgt caaataggtt tttgataagc
    60721 caacgtcatc aaaagaacgc ttgaatgttt ctcctggaag aatgatggac agatactgtt
    60781 ctccaacgat cagattgatt ccgatacagc tcaaggcagt aaacccgact aaacgagaag
    60841 gtcggtccat cttttctttt aattcttgaa cgatggtatc aacgatcaga tatttgatca
    60901 gtaacccacc taatgccaat gcaagtaaaa tcaaactagc agaacctaac atactcatga
    60961 tcccgcctcg agaaagcagt tcatctagct ttggatcagg tgtatttgcg acataaccat
    61021 tcatcaagat gtctgctgct tttccgaccg tcaaactttt ctcatggata aaagaaagaa
    61081 cgagagcagc tgtcgatccc gctagtaatg tagggatagc cggaattttt ttgattgccc
    61141 ccccaaataa aataactagt ggaatcaatg tccaaggaga gatccaaaag ctcgctttca
    61201 tgttttggat catttcattg atactagcta gatcggctga atgactgtta tgtcctaata
    61261 tcgtataaaa aatcaacgaa aggacgaatg ctgggaaatc cgtataacgc ataccagcga
    61321 tatgttcgaa tagattaact cctccaatac ccgctgccaa gttggtcgta tctgataaag
    61381 gcgagcagtt attccctaaa aaggcaccgg aaacgactgc gcctgtagtc attgcatcat
    61441 tgaatccaag taaatgaccg atccccaaaa aagcaattcc aatcgtcgaa atcgttgtaa
    61501 atgaactgcc gacagtcact ccgacaattg cacaaacaag aaatgctgtc ggtaaaaaga
    61561 aacgcacaga aacaagttta aatccataaa ccataatcgt agggatggtt ccagataaaa
    61621 tccatgaagc aactaagaca ccaatcaaaa gaaaaatcag gattggaatg atccctggag
    61681 tgattccttt gatgatcccg tcatggatct catcccatgt gaacttttta aatctgccat
    61741 aaaacaacaa acacatgaaa gctgcaagga tcgggatatg tggcgagagc tggaatccga
    61801 tgatcagtac tcctaggagt gccaacaaca cgatcaatac agcgatactt tcttttaaag
    61861 aaatattttt attcatcgtc gatctcctat ttcaaatgaa aaggccctgc catgaaaaaa
    61921 ctattcactt ggcaggacct cctactaaat cagcgacaaa acgtcggctg aaagacttat
    61981 ttttttattt ttcttctaaa cttttttcta cctcaatccc ccaagaatct ttcttgaagt
    62041 gattgatgat caatggaata acgaacatga ccactgtgat agctgctact aataatgcat
    62101 cactcaatgt cgctgtacct aatgtagcaa atacgacaac gatgatagag aagatcagca
    62161 tgcagctgac tacccaagct aatccgtttc cgtttttacc aattcggtaa ggacgttcga
    62221 attctggatg tttcttacgc attttaagca atgaaacagc aatcaaaatg tacacgatac
    62281 aataaataac agttgtagca tttgtaagtg tcaagaatac agcacttacg tcatccatta
    62341 caccataaag caaagcaaat aatgaaataa cgattgattg tgttagaacg acatttctag
    62401 atacgccgta tttgttttct ttatggaatc cgaattttgg tggaaggaat ccttcacgag
    62461 ctacttggat gattgttttt gatggtccag taacccaagc tgataattgt aaaagcacac
    62521 caataaatac catgaagctg aagatatttc cgatcacttc tggtaagcct aggatttgac
    62581 agtcaatcaa gataggctgc gtgatatttg ctaattccat ttttccatct ggtacgacat
    62641 ttgctacaag catcgcattg atgacattca ataaaactaa tccgatcaaa gcgataaata
    62701 cgccttttgt ataatttttt gttgcgtctt tcaaacgagg catgtaaaca gaactcattt
    62761 cgatcccaac aaaaataaat gtgataccag ctaagtattt caatgaatca atatgttcca
    62821 agtcagggaa agcttttgac catgaaaagt cacctagata gccgtttgag actagtccga
    62881 ctttgaaggc tgccaaaaca cctaaaacga acatgatgac tactgggata tataccccta
    62941 gccatacacc gatgttcccg ccgacttttg ccatatcgaa tttcaagttc aagatcgtga
    63001 tgatccagta gataactaaa atacaaccta aaacgaacca gtgattattg ccaagttcta
    63061 cattaccaaa tgtgtttcct aacaatggtc ctaaagtaga agcgaccatt accatacctg
    63121 ggaacatttg cacccataat agccatgcaa cgacaaatcc ccatttgctg cccagtgctg
    63181 tttttaccca taactgagga ccgccttctt gaggaagcat cgtggaaagc tcaccggaaa
    63241 ccattgatat cggaccggca aaaaacaaaa ctgcaaaaat tgaataaaaa atcaatgtcc
    63301 aaccgactgc ggctaacgta ggaatactcc gaaccgttgc acacagtgcc attgtcagac
    63361 cgataaaggt cccaagaccc attttactta cttcagattt actcaccgtt gacactctcc
    63421 ttgatttttt ggataaaata acggtgtctt tgaagtagtt tcagcaataa acgaaacaat
    63481 tcagcgaata tttgagggga ttctttgagt ttattgcttg aagggtttct tcaaagacaa
    63541 cctcaaaaca aagggatcga agagcttagg tctccccttg ctcttcagat ccaaacatgc
    63601 gtcagaaaca gttagtattc aagctgtttt ctaacatgat taccttgctt attttgcttc
    63661 gcttgccaat aattggtctg cgtagatttt ttccaatttt tcttgaagag ctgctttgat
    63721 tttttcagca tattcttcaa agtgttcttc tttgttcatg tatggtgtca ttacgctggc
    63781 acgcaataca gttacttttc cggcacggtt ccattcttca tctgagaatc ctagttggtt
    63841 cacaaattgt aatgggctgt tgccatagtc tgggatagca aagtcagtat gtgaagttaa
    63901 gaactcatta ccgtaaatgc ttcctttcac gtatgaagaa taatcatata cgtcatggtt
    63961 caatttattc attgcaacta agtcatcgtt gcctttttct ttgaatacat agtctaccat
    64021 attgaaatct ggataagtta atggatgaac ttcgatttct ttgtctccta ctttaaatga
    64081 caagtcattc aagaagttgt agaaacgatg tgcaccttcg attgatgcac ccattaattt
    64141 accatatcct gtgacattca aaggtaagac atggtgtgct gcccaaacgc tggctgctgt
    64201 tgcccctgct tttgaacctt ccaagatata agccccaagt aatgctggga tatctgcgcc
    64261 tttttcaaat acatatgtcg caaagtaaga gataacgttt ctcatgcgga tatctttgat
    64321 gacgatacca ccggcagaat atggaacata gcccatttta tgtgggtcaa tcgttacaga
    64381 ttctgcttct tcgattgctt tatatgcact gtgtacttct tctaagatat aatctttgtt
    64441 ttctgtaaag acattgtatt tgtaatgaac atcttttaaa tcttcaaatg gaatgaagtt
    64501 gttgtcttcg tctaagaaaa tcgcacggcc gtatccgcca taagcagcat ctacgtgtaa
    64561 gtagaagtaa ataccgtctt tttctaatac gcgacgcaat gcaacgattt tatcgatacc
    64621 gtcgattgct ccttcttctg ttgatcctac aacaccgact acacctagga ttggtgtttt
    64681 ttcagctgct agaccacgaa cgattttttc aagttcgttg atgtccatgc gatagttgtg
    64741 gtcaactggc actggaatca cttggtctaa accgataccg atgatgtcgg cagcttttag
    64801 ccatgaatag tgtttagttt gtggaaccaa ccattttcct aatttttcta aatgttttcc
    64861 gctacgtgct gagtgagctt tgatttcgtc gattttttct gggacgctgt ctaacagatt
    64921 catgatttct tctgttgaca agttcattaa ttcccaatcg ctttttccag caactagttc
    64981 aggtgtcact tctttcattg caagcggcaa tgatttgatg ttgcgggcat accaaagtcc
    65041 ttctaagttt gctaaagaac catcagcaac gatgtggccc cagccatctt tatagctcat
    65101 taattttgca aattccattc ctacttcttc ttccatttgg cttgttgcag gagaagattc
    65161 ataggcaacg ttattaccat tccatagcat agcaaaattg tacgctagta aagatggcat
    65221 caatgtttca gagttcatat gaccccagta acgtccggca ttatgccaag gaacggaatg
    65281 ggtacgcatc cgcgctgaaa tttctgatag aacatccttt gttttattca cagtgtgttc
    65341 aaaactagca ctgctttttt cttctggcgt aataataggc atgtcttgag gcatgtagtt
    65401 ttgacgccag cctaaatgtt catccaccaa ttcattcaac aaagctttat agatttggcc
    65461 gttctcagct ttgtccccaa tgaatagggc atttaggttt agatctttcg acaatgattc
    65521 actcatagtg aaaccctcct aaaaaaatat ttaaattttg tcgagcccta cattacacct
    65581 gttcttctat tctgtaaact aagttttggc ctgattttta atcatatttt aatatgtatc
    65641 aattatccga attgttcaca cttttgaaat aattaaaaaa aacgagggaa tttacaaatg
    65701 taaactccct cgcaaagatt gtattaacga caaccagcgt tctattttag gatgggtttc
    65761 aaaattggaa tgttgtaata gtatttccct ttttcaaggt aaaaacttac taaattctaa
    65821 tcaattactt tggccaaaaa gtaatttttc ttaccttttc gaataaccac aaacttacca
    65881 tcgaattcat cggatggatt tatgacaaaa tttaaatcgg taacgcggtc accgttaata
    65941 cttatagctc cgtttgaaac atcttcacgt gcttgacgtt tagatggttc aatttttgtg
    66001 gaaacaagca gttccacgat attttcaggt gtgcttgaaa tttcaacgtt cggcatttta
    66061 ccgaatcctt gcgcgatttc ttccgcattt agttctttaa tatttccaga gaacaatgct
    66121 tgcgttattt tttgcgcttc tttcaagtct tcttcactat gcacaaatct tgtaacttct
    66181 tctgccaaac ggcgttgtgc ttcacgtttc tcaggttctg tctctacttt tttcgctaag
    66241 tcttcgattt cttcttgaga caagaaagta aagaatttca agtatttcac tacatcgcga
    66301 tcatcttgat tcaaccagaa ttggtagaac tcaaatggtg aagttttctt cggatcaagc
    66361 caaacggcac cgcctgcagt ttttccgaat ttcgttccgt cagccttcag catcaatggg
    66421 atcgttaaac cgaacgcttc tgcttctggt ccttctaatt ttcggattag gtctaatcca
    66481 gctgtgatat ttccccattg gtctgctcca ccgatttgca attgcacatc gtatgtttta
    66541 tgcagatgca aaaagtcgat agattgcagg atttggtaag taaactctgt aaatgagatt
    66601 cctacttcca aacggctggc aacgatatct tttgctaaca tcgtgttgat gttgaagttt
    66661 ttaccgtagt ctctcaaaaa ttcaagtaaa gagatttttg acaaccagtc gtagttgttc
    66721 acaaatgtga tatttgcatc tttaccaaac aattttctca tttgattgga aagtgcgtcc
    66781 acgttatgtt gcacttgttc catcgtttgc aacacacgtt ctgttttgcg gccgcttggg
    66841 tcgccgatcg ttcctgttcc gccgccaatc agaatatatg gatgatggcc tgccaattgg
    66901 aatcttttca tcatcataaa tggaatcaaa tgtccgatat gcatcgaatc accagttgga
    66961 tcaacgccgc aataaagtga aatgctcttt tcttcaacga gctcgcgaag tctttcttcg
    67021 tttgtttgct gattaatggc gtcgcgccaa gttaattcat caataatatt cattttactg
    67081 acctccgtgt agttttttta tgaaagcagg aacctgctag catatgtaat taattatttc
    67141 ttcaggatag gataggaaat aaaaaaagtc ccttggcata taagccaagg gacgaaatct
    67201 cgcggtacca cccaatttgt atgtctagat atccaacata cctctcgaac atttaacgct
    67261 gtcgcgtggc aatcactgcc aaagactcct gaaatgtaat tcgctttaaa gcatgtactg
    67321 gtttgcagcg accaccagct ttctgaaaca ttggctttaa caactacttc gtttcgatct
    67381 tcgtcttgtt attattaata tgtgtctatc atagagcgca actttttctt tgtcaacaga
    67441 aaaaggatat tttttttaaa ttcctcattt tttctttttt gaatccgctc taattccgaa
    67501 cattttattt tcaattattg ttaaaagaaa taactttttc tgtatttttt ctgcaattta
    67561 tcgagaagtc actttacttt gcctcttatt ctttcttcgt tacaataagg aatgggacaa
    67621 gtaaaaaaat gaaaaagcaa gggacgaggt atatggaaaa aaagcaaaaa attattttag
    67681 gagccacttc tgctttaact atcgttattt taattctaag tttgtttttt gtcatccgat
    67741 catctacaaa aaaagaacca atcattcctg aaaaaaaaat aacagaaaaa gcaaaagaac
    67801 cgacagatga taagcaacaa ctagttactc aagcaaaaaa tctggctcaa agctacgatt
    67861 atgaccaagc gattgcgtta ttggaaaaag atagtaagaa cggatctact gaaacacaac
    67921 agcttcttca aacactaaaa aaggagaaag aacaactgat cgactggtct gatcctactc
    67981 agatctctca tgtctttttc cacagcttga tcgtggaccc gcaaaaagcc ttccactctc
    68041 aacaggcaca aggatacaaa gattatatgg tgactgttga agaattcaac cgttcgatcg
    68101 aacaacttta tcaaaatgat tttgttttag ttaatttgaa tgatttgatc caaaaagacg
    68161 aacaaggaaa tctttccttt aacgggcttg cgctacctga aggaaaaaaa ccattggttc
    68221 tttcacaaga tgatgtcagc tattatgaat atatggataa ttccggtttt cctagccgat
    68281 tggtcgtatc aaataacaat gaaatcaaaa atgtctataa agagaaagat aaagaagaaa
    68341 tcggtgactt tgacatggtt cctctgatcg atgcctttgt caagaaacat cctgattttt
    68401 cttatcgagg cgcaaaaggt accttggccc tgacaggtta caacggagtt ttaggttatc
    68461 gaacatcaaa atctcaatat ggagacaatg aaaaaacaaa aaaagagatt aaagaagcta
    68521 aaaaagtggc ggaacagttg aagaaagacg gctggagctt tgcttcccat acatggggtc
    68581 acttgaatat gactcaagct tcactagaag atgtaaaaaa agacaatgaa ttgtggcagc
    68641 aagaagttgc acctatccta ggaaaaacaa atattctgat ttatccattt ggtgcagaca
    68701 tcagcgactg gcagccttat tcagcagaaa atcaaaaatt tgcttacttg aagagtcaag
    68761 gtttcgatat tttctgtaat gtcgatgctt ctacaccagc ttggggacaa ttaggtacgg
    68821 atttctatcg aaatgctcgg atcaatatcg atggcattcg ttttcaatct gatttagacg
    68881 gaaaaaatcc tattcttgat ccattcatca atgtccatga tgtctatgac aaagaagcac
    68941 gcgaatctgc ttgattttac gtcatttatg aaccaaaaaa gccgaactga cattttatcg
    69001 tcagtttcgg cttttttatg ctttatttct tcttcggtgc gattcccttg atcaggactt
    69061 ctgtccattc tgctgtatat ggaagcagtt ttgcgtatat ctccggctta gaaattcctt
    69121 ctaccattgc ttgaaaatag aggatcaaga actcatccga atatttcaaa tccacttttc
    69181 cttctttccg ccctcgatgg aaaagatcaa gcatgatact gaaactttct tgcgtatacg
    69241 catccatcgc gtcagaaaat tctttttttt ctttttttgt atagtatgtc atcaaatcga
    69301 tataaaattg tttgttcact cgattgagat aacggatttt gttttcactc atggcaatca
    69361 gtgtttcttc aaatggaaga ttttccgcaa tcaacgcttt cgcttcttgc cctaaatcat
    69421 tcataaatgc aaaaaatact tcataaatca gcccttcctt gctgccaaaa tatttaaaaa
    69481 tcgtcgcttt acttacctga gcgttctcag ctaccttatc cattgtcact ccagcggttc
    69541 ctatgtctgt gttcatcaag tcaaaagctg cttgtaaaat ctctttcttt ttctcttgtg
    69601 ctctcttttc aaaaccgttc atttgtagag cctcccgtaa ttttaaactt ttataatata
    69661 tatagttcaa atttattctg aggtttatta taactatcta ttgagaaagt atcaagctag
    69721 caattccttg tttatgaatt cattgacaga aatttataaa aagaatatta tgtactaaaa
    69781 taataaaaaa agttttataa aaaggtataa aaccttttta ttaaactatt taatcttgtt
    69841 tagtatatat ttttagaaaa aggagtgggg tttttatggc agaaatcgtc aaagtcacac
    69901 atttgaaaaa gagctttggt cgtttcgaag ctttaaaaga tgtctctttc accgtacatt
    69961 caggtgaagt cgttggattt atcggaccaa atggttctgg aaaatccacg acgattcgga
    70021 cacttttagg gattataaaa aaagacggcg gaacggcaac gatttttggg caagatgtat
    70081 gggaagatag tttatctatc catgaacgtc tttcttatgt tccaggcgat atctctttgt
    70141 ggggaaattt gactggagga gcgatcatcg atctcttcat gaagcttcat ggaaaaggga
    70201 gtgctcaaaa aaaagatgaa ctgattcaac gtttctctct tgatcctaag aaaaaagcaa
    70261 aaaactattc aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg gctgttgatt
    70321 cggatctcta tttatttgat gagcctactt ctggtttaga tcctttgatg gaaacgattt
    70381 tccaagaaga agttgaaaaa ttgaaagaac agggcaaatc catcctactt tcttctcata
    70441 ttctcagtga agtcgagcga ctagcggata aagtcgtgat catacgcgaa ggaacactcg
    70501 atgaagaagg aacactcgat gaattgcgtc atttgactcg ttcgaccatc actatagaaa
    70561 cagaagcaga gaatagtcac ttagcacaat tgaacggtgt ccatgacttt gtgcaaaaag
    70621 gtcacctcgc tcaatttgct gctgataacg aagctttcga tgtgatttta gctgaagcgt
    70681 ccaaattagg tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat ttattcatga
    70741 gacattatga aggatgagga ggaagcgaaa tgactgataa attttcacgc tggtccaccc
    70801 ttttttctca aaaccttagc cgtgactgga aaaagatcct gatatggcta gtcggtgtca
    70861 ctgctttttc tggtggtttt gtccctgcgt ttgaagaatt gacttccgga caaggattag
    70921 ctggattata cgaaaccttg caaaacccgg caatgattgc aatggtcgga ccaactccag
    70981 cttcgaatgc tgctgagtat actttaggag cgatgtatgc aaatgaaatg ttgctttttt
    71041 gcagtttgtt tgctgctgtg atctctgttt tacacgtggt gagccaaacg agaaaaaaag
    71101 aagaacttgg tctgattgaa cttattcgtt cctttcgagt cggacgtcaa gcaaactctt
    71161 tagctgtcat cgctgaaagt attctgatca atttgctttt agcaatatcg atcagctgta
    71221 cgatgatttt tttccaagcg gattcgatca ctgcagaagg tgcttggctt tttgggctat
    71281 ctgtaggtag tatggggatt ttggggagtg ccgttgcact gtttgctgca caactgatgc
    71341 caagttcagc cagttcgaat ggtctgtctt tagcgttttt aggtggactt taccttctac
    71401 gagcaataac agatgtagct gattcctccc tttcgatgtg gaatccttta ggctggtctt
    71461 atttgaccta tccatttaca actaacaact ggattccttt gctttatttg tttttgttca
    71521 gtctaatctt gataatcagt tcatttgttt tagaggaaca tcgagatttg aacaccggct
    71581 atatccccga acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc ttgcgtggtt
    71641 tattattcta tctaaataaa gggatgacaa tcagttggct tatcactttt ggtttgatgg
    71701 gggcggcata cggctctatc tacgggaata tgcaacagtt tttagaaagt aacgacttaa
    71761 tgaaacagat gtttatccaa tctggcgtct caattgaaaa aaactttaca agcacgatca
    71821 tggtagtctt gatttgtcta gctgctattt taccaattgc tagcatcaat cgtttattta
    71881 cagaagaaac ccacggacgt atgaatcagc tgttcgcaac aaaaatttct aggaaccaac
    71941 tttattggac caatatgtta ctagctattc tatttgctat attggcaatc ctgattagtt
    72001 ctggtgcact agggctttct gctttggctt cgatgaagga tgcgaaagac atggatctat
    72061 tgatgtttct taaagccggg ttcaatttcc tgcctgccgt tttagtgttt atcggaatta
    72121 gtggctttct attcggatgg cttcctcgaa tcggtaaact tgcttatatc tacttagctt
    72181 actcctttgc actcaattac tttggcggaa ttttggattt accagaatgg ttttccaaaa
    72241 cagctgtccc tagctgggtt ccacggatgc caaacgattc ttttgatctt tccgtgttcc
    72301 ttacgttatc atgtatcagc cttgcactgt tcgtccttgg ttctattggt tataggcgaa
    72361 gagacttact agaaggataa taaaaaagca tgtacaattt catgctagcc tccaaatctg
    72421 ggtcaaaaat ccaacatttg gaggtcattt ttttatttac aagtcttcta actgctcttt
    72481 atcaatgatc cagacttctc gattctttct ttttacataa ccattctcct ctaataattt
    72541 aaattttcta gataaagttt cgggggtcgt accgataaat gcagcaagtt ctttcatttt
    72601 cattggcagg aaaaagtgat ccttccctgt cgctttagct aagtcaagca gatagacagc
    72661 gactcgttct tctacttttt ccatcatcaa aaaagttgtc tgctgctgta cttttgttaa
    72721 tttctcaacg ttgattgcta aaagtttcag actaagggcg ggataatgca gtaagagttc
    72781 cgagaaatct ttttgtctca atatgcaaat ttccgttttt tctagtgctt cgccaaataa
    72841 agactcattt ggaacgccta gcaactggct ctctccctca tatccgcccg gctctattac
    72901 tcgtaaaagc tgttcctttc cgctagcaga taattgatac actttcatac tgccggaggc
    72961 cacgatcact aattgaggtt ctccattcgg cgcaagaatc tgttctcctt tttccacata
    73021 gtgatgtttg acaagtgcgt gtactttttc ttgatcttgc tgtactaagt gattgaacaa
    73081 aggaacaaat gaaacacata aatgatcatg actcataatc tctcctcctt ctaaacagct
    73141 atttctaata aagaaacata acttccctta ttccaactga taattttctt ttatcatagc
    73201 ataaacgtca agcgaaccag ataccagcaa atagagtgga tttctttttg tcccttgctt
    73261 tattttcctt cttttgagac cttaagtaat tgattcctat caatttttta aatatttact
    73321 ttttgaataa tcaaaaacaa accttcactc gcaagccgag agtaaggttt gtttttcgca
    73381 ttgcttattt cgtgttgtct attgaaattt ttccgcacct agcttttgat atagtggtgc
    73441 tagttcttgt aaggtttcag cggttatttg ctcaacgtgt tcgatttctt gcggatagaa
    73501 gaatagttta cccatttgaa aggttcggtc tctcatcgct tttttcaact cttcttgttt
    73561 ctttgtatcg gacaaatagt cagacaaagc taagtggtcg tcaagttcat gttctggttg
    73621 cggccatatg taataatcct tgatatcgac tgttttgctc cactctttca gtatcgcaag
    73681 caactggtta tacgctgcaa tgctaccata tcgttcttcg cttttataat gttggtacat
    73741 caaatagatt tggtattgtt tttgattcaa caaaactgct agacaggcat tctcgtcttg
    73801 acgagattcg cttcggtacg cgctccaaaa atgactgcgt aaattccagc cattcgtcca
    73861 gctttcgatt ttaggtttgc tcatgccgta catatcaggc aaaagtgcag ctgtctggag
    73921 ctgaatctct ttccattttt gccagtgttc tttatattct ttcttgattt gttcaatctt
    73981 atctgttgga tggctctctt tgagctgttt gaatgcgtag attggtcgat caaacaactg
    74041 ataatctgat tttccaagtt ccattgtttc gcctctttcc gttatctttt gtattctacc
    74101 tgaattttgc gatatggaca aatagaggtt gtgaaaaagc tgttctgcat caagtcataa
    74161 gaacagccaa acaaaaaact gcgatgagca tcaccgcagt tttattgaac ttaaggttga
    74221 ttgatcacat ctaaaagcgt tgaaaataaa atggcactag aagcagctcc tgggtcgata
    74281 tgtccgattg cacgctctcc tagataggaa gctcgtcctt ttttagctac caagactttg
    74341 gtgtgttctt ttgcctggtc aatgatttct tttgttaaat tccctgcctt caatgcctct
    74401 gtgactggca gccaaacatc taacatcgtt ttatcgtcag cttctgcctt tcctcggctt
    74461 tgaatacccg ctgttccctg ctcgatcact tcgcctaatt tttcttgaga atcaatagaa
    74521 tcgacctctt ttgtcgcttt catcatgttc atgaatgcag atccgtataa cggaccagaa
    74581 gccccaccga ctttcgatat catcgccata gagaatactt tgaatgtatc gctgattgtt
    74641 tcaggatgat cggcttgaaa agcttcttca taagctgaaa cccctcttcc catgttattt
    74701 ccatggtctc catcgccaat cggggtatct agatcactga gatattgttt gttctgattg
    74761 attgcctctg caaattgcgt gagccattct tggatttctt gaacggttag tttcatatta
    74821 tcaccctttt atcttgagat tttttaccat gaaatcgtct gtacattgct ttctaaagaa
    74881 tctttccatt gtgtctccgt caagtcgatc agtgttagag aaagaccttg catatctagt
    74941 gaggtcataa aattaccgac ttttcggaaa actacattga cgcctctttc ttccaacaga
    75001 gctaaaacat cattcatgaa cacgaactgt tccatcaaag gcgtcccgcc cattccgttg
    75061 actagtacac ctgcttcttt tggtaaattc gagtaatcat ctaaaatctt agtaacaagt
    75121 tcctttgcca gtactttaga aggctgcatc ttttctcgac gatagcctgg ctcaccgtgg
    75181 ataccaacac catactcgat ctcatcttct cctagttcga atcctggttt ccctacttct
    75241 ggtacagttg ctgctcgaag cgctagaccg atcgtcttag ttgcctgtac gaccttttct
    75301 cccagttctt tcaactcagc tagggaagca ccgtttcttg ctgcatcgcc cacgattttg
    75361 tgtacaagga cagtacctgc aactccgcgt tttccagcag tatacgtact attttcaacg
    75421 gcgatatcat catctacgat cacactttct acttcaatat cgtccatagc agctaattcc
    75481 tttgccatat cgaagttcaa tgcatcccct gtataattct taacgatcag caaaactcct
    75541 tttccactat cggcttcctt aatagccgtc tgaatctgat ctggagttgg agaagtaaac
    75601 acatcaccaa gcaccgctgc acttaacatc ccttccccta caaacccggc atgggatggt
    75661 tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt ccgctttctg attcttggct
    75721 acaacacggg aagattctac acgatgaaca agttctggat agctttttac taatccttcc
    75781 agcatttctt cgacaatatt gcttggatca ttcatgattt ttttcaattt attcccatcc
    75841 tttctttccg gcattttgtg cttctgttaa gatttgctga gtatcatctg ttataccggc
    75901 agtgatggct gctgcaaaag ctccttccac taaaggcgca tccaccagat gataacgttg
    75961 ctgctgctct tcttccaaca tgtcttttgc cagttccgag ttcaataccg cgctaccgat
    76021 atccgcaaaa acaaaataac tagctgcatc gcttgattca ttgactgctt ctacgatttt
    76081 catcgggtct gatccgatct cgccatttat tccgccgaga gaaaaaattt gcacctcttc
    76141 ggatttttgc atctgctcaa tcatttcttt aataccatct gttacttttt gactatgaga
    76201 aataagcaaa atactttttt tcacaagaat cttcctttct tttacatagt tgctaacgat
    76261 tggtttcgct ttcattaatg aacaattcaa gtatataata aatttctaat tttgggaaaa
    76321 tatgtgtagt gcttcttgaa gtttgttata ctttaattaa aagagaggag tgatcgaatg
    76381 attacaacaa caacgaacag tgtagaggga aaagccgtag tagcttataa agggatcgtt
    76441 tttggtgagg tcattactgg gatccatgca tttaaagact tggaggcggg gttgcgaaat
    76501 atctttggcg gccgctcaaa aagctatgaa aacgaactaa tgggtgcaag agaagaagca
    76561 cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg atgcgatcat tggcgtaaaa
    76621 atggactatg aagtattagg cagcgacaat ggcatgctga tggtcacatg tagtggaaca
    76681 gctgtctcca cgagataagt aggggacatc tgttttaata actaaagaga gatgggacat
    76741 gtagtggcac tcaagaatta gatttttcag tctaacttcg aggtactcta ccttcccatc
    76801 ctctcttttt tatttctttt tgcggatgat tccgtaagct gctgcgacta acacacccgc
    76861 ccctgctaag gaataactga cagaagctgt tttttcattt gtttttggca aagtctgact
    76921 tgttgatttg gatggctctg tcgtttgctg aactgtttct gtcgtgcttc cagaggtcac
    76981 tgatttttct cctgccgaat gcaaaatatc ttttattcgt tggaaactag ctgctaagtc
    77041 agcttttttt gcttcaaaat cattagcaac tgtttgtgct tcgctgatct ttccgtggat
    77101 tgcttgactg gccgctgtga tatccgcttg agtttgactt agctttccag aagctgtttg
    77161 agcaacctga tttaattgct gattgaattc ttttgcttgt tggatcttcc cgtcaagatc
    77221 tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc tttttctcaa gttgtgcacg
    77281 gtcatttccc actttatttt taagatcagc taaatcttga tcgattgctg cttggtcttc
    77341 tttcaatttg ttttttgcag cttctaatgc ttcttcgagt tcttttcttt tattttccaa
    77401 gtcttgcaaa tgatcgattt ggtcagaaga ggtagaacta tttattgatg tatcttctga
    77461 agatgaaatc gtttctcctg aattatctgt cggtacattg gaagtcattt ctgtttccga
    77521 ggcaccgttt tcagcaccag aagagcttat ggttgttgaa ggttctgttt gagcaggttc
    77581 agaagtttcc gcaaaagctg tctgtcccaa taagaaaata ctggaaacag caattggcca
    77641 gattaacggc ttcattcttt ttttcatcac ttttcccatc cttactactt tttaactaat
    77701 ttcttattat aagcaaaaat gtttggaaga accataaaca caacaggctg tttgtctaaa
    77761 aaacggtgca tcttttattt ttttgtacgt tccgggcttt tccatgtccc gcttccataa
    77821 cctaaaatcg ttttgactgc tggaatgaaa gtcgtcacga tcgttatcgc gttcgctatc
    77881 cagaacaaaa gcatatatag tggtgcgaaa agaaggtatt tcaattttct tcgtcgatca
    77941 tctacaatca gcgaagctag caactggaaa aatcctgcga ccatttcaaa gcagatgaac
    78001 acaaaactga atgtaaaagt aatataaatc tgttcgtagt ttccttgata tacataatgc
    78061 ccgattaatc cagcaaaaag aactacggaa atacaaaaga atattgacca gacaatactc
    78121 aatgtttgat caatgaagat gatcgttctt ccaatatgtt taaaaggatg gagcatcact
    78181 tttttgaaat tcgtcagcca gacttctgtt ccaccttttg cccaacgttt tctgtgtcga
    78241 tatagcatct tcaacgtcac aggtacttcc atgaaaaaga taatctcagg agcaaaaaca
    78301 gataaccagt catttaactg atgatcccaa gcaatactga tgtcttctgt cgctcgatct
    78361 tgtctgaatc cgcccacatc gatcaaggct tcttttcgat acatcgtatt tgctccactg
    78421 tacgcataaa gaccaccgaa aacagctgac tgtgttcgtt taatgatgcc cacgatactt
    78481 gagaattcaa ccgtttgaga ttttgcaatc aatttggtac gattttggac atccatattt
    78541 gcagtgaccg cagcaatatg tcttgcacca gggcgaatga aataattcac atacctaatc
    78601 agtgcatctg gctccggtac cgtgtctgca tcattgctta aaatcaattt cccttttgca
    78661 aaggcaagtc cgatattaaa cgcgtgggct tttcctttat ttttttcgat ccgaaccaca
    78721 cgaagattag cgtatttttt catcagtcta gctaatattt ctggtgtttg gtccgttgaa
    78781 ccatcgtctg tcactaatac ctcatagttg tgataattca atttcgtcat caaatactcg
    78841 attgtatatt cgatcacaat ttcttcatta tgggctggca ccatgatcgt gatgaacggt
    78901 tcgacactca agggaacatc tacccattct ttttgcttat gtttgtataa aaacacataa
    78961 caccagacac caataaacca tacaaatccg cctaaaatcg gataagaaac gagaaaaaaa
    79021 tacataatag acaaacaagt atctaagaat aaattacctg tatgggtgat tgagatcatt
    79081 tcagttccac cccattttct ttatacaaat ctgcaatgaa tgtcgtatct agatttttct
    79141 cttcggagac agaataaaaa cggaccgttt ctctctcttc ttttgttcca aaacgttccg
    79201 tatacacttc ttcaagtaat tcttgtcttt ttttcaattt ttcatcttca tattcaaaag
    79261 atttatttac ttttttgtat agatttcgat tgttccacct agtcaataca aatgaggtaa
    79321 tcaggaaact gaaaaaaatc aatacaaaaa agccgattaa gaaatacagc attttctttt
    79381 ctgataaaaa agcgtacaag gaaaaatcct tggctctttc tggtctcgtt aatgggaaaa
    79441 agacccatat aaaaggaaga acaacgatca tccatcctaa aagagccact aacgtttgcc
    79501 aaatctttaa ccagtaatta ccttttgaaa aatacttatc tttcaccaaa attgatgcaa
    79561 cttcttctct tgttactttt ttcatatcct cacctcacgt ttttttgtta cgacgtagga
    79621 gtttaccata catttcattt cttttcacat aaagtgttcc taaatttttc ctaagaaaaa
    79681 gaacaaaaca tttctcatca gcaaatgaaa aatattttgt tctttttatc agttgttctt
    79741 tttatttttt atctcttaat tatgttgctc tttccaatac gacaatgctt ttcattcctc
    79801 tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc atctccatcg ttttcaaatg
    79861 gatataagat tacatgatca aattccagtc cttttgctag actcaaagga atgatttggc
    79921 agttttctaa tccatatgaa ttttttttaa tcgattcttg tagcgtttgg gtttcctctt
    79981 ctgtttgggt gataactgcc gctttttctc cttttagaga aaccaaaatc tcagctagta
    80041 ttttcaacaa ttcttcagag gatcgaaaag agcggtattc gggttctttt ccttgcggtc
    80101 ggatcgatac gatgtcgatc ttctcttgat ccacagcata ggttttgaaa agctctgtga
    80161 ttgctccgct agaacggtaa ctatttctaa gatcataccg atggatcggc agatggtagt
    80221 cgtcgaaaca acgcataata tcagcaaaag tagtggaaga attaaagatt gcctgattct
    80281 cgtcacctac taaagtaaag tgagtttttg gaaataattc gatcaataga cctaattgtg
    80341 ccagggtata atcttgtact tcatcaatca atagatagcg aaatgctggt acatttacct
    80401 tttcaacaag taaatgacga atagctaata taatgactgc ttcatcaaca gtacgtgtgg
    80461 gttgccaagc ataagctgag ccatatcgtt tttcataaat tctttcaaac aactgatgct
    80521 catccaccca tgccatttct tcgatttgtc tggttatttt tcgatatttt ttttgtaaaa
    80581 gtttccttgc ataagctaca atactttgtt ccgaatcatc agaaatcaat ttaccgaata
    80641 attccaactg cctatcttct gtcaacgaga gtacctgatt ctgcacagca ggactttttg
    80701 cttgtttcaa aagatgattc ttccattcgc tcatcagtgc ctttttcgtt gcttgaagcc
    80761 tatcgatcaa tttaggatga gcaggaactt tctcatatat tttttcgatc gtttttttag
    80821 aaaggactgt ttttccattt ttagtaagtc cacggataaa attcggatga tctaaaaaca
    80881 aaggatctga ttgttttaga ttatcgataa actttttgct acgcaaacgt tctgtctgct
    80941 ccgatacatt ttctcccgtg atacgtttga aataagcctc ttcaccttct atttcctctg
    81001 ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag tggatttctt tcacctaatg
    81061 atggtaggac attcgaaata tagtcgataa accgatgatt aggtgataga atcagcaagt
    81121 catcggatgt catcgtatct cgatataaat agagcagata cgcaatacgc tgcatgatag
    81181 cagaggtttt accacttcct gctacgccat ttaccaagat attttgactt ttcacatccc
    81241 gaatgattgt gttttgttcc gattggatcg tagcagtgat agccttcatt tcattggtct
    81301 catcttggcc taagacttct aataatacgt catcttgaat agctacagac gtatcaaaaa
    81361 acttgaccaa acgatctctt tccaaaagaa attgtcgtct ttctttaata accgtatcga
    81421 ttttattttg acgaaccgta taagaagatg gaccaatcat gttattgtaa aaaagttcgg
    81481 caattggtga acgccaatca taaacaagat tttcatctgc atcatctgtg aaatttgctg
    81541 taccgatata aaaggattct tttggctcat tatccagaaa gtctacaacg acttttccaa
    81601 agtagggact tttcaataaa cgttcgatct ttgctagctg attttctgcg tttttctgtt
    81661 tgatattcat ctgatcgatt tctctatttt tcatttcaat catcgaaaaa gtatccaggt
    81721 tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc catttctttc aacgactggt
    81781 ttccttctat ttttgccgta tccatcgatt cccgcagttc gtttttttta tcaattagct
    81841 tttgatacac gttcttcaga tatgcttctt ctaactgttt ttgtgtgttg accatacaca
    81901 ttctcctctt tttttaaagt ggtggaaccg attagtttac ctcttttaag agtcgaatgc
    81961 aagcttttta acgagtatca tttgttagga tatatcagtt cttcaggtcg gacagtttga
    82021 tataaagaaa atgcaccatc aaggtttttt acgtggtagc cagactgctt caagattctt
    82081 tccgctagat agctacgtaa tccgctatga cagctgacga tatattcttg ttggctgtct
    82141 aattcattta atcggtcccg aagttcattc aaagggatat gtttggcttc cttgaatttg
    82201 cccttttcca attcttctgc tgttcgcaca tctaataaaa tttttccttt tgctagttct
    82261 gtcggcaatt catgccattg tacggtttca ctaagccctt ctgcaatgtt cattgctgca
    82321 tatcctagca tgtttactgg atctttggct gagccaaatg gcggtgcata cgtcaattct
    82381 aattccggta aatcgaagat cgtcaaatgc cctttgattg ctgttgctaa aatatcgatc
    82441 cgtttatcca caccttttgc cccaacacct tgtgcaccat aaatctcgcc agtctctgga
    82501 tggaagatca acttcagtaa aatatctgtt gcttctggat aatagcttgc atgatctttt
    82561 cctgatacat gaatgacttc aaaagacagt tcgttttctt tggctgttct ttcacttaaa
    82621 ccggtggatg cggcagtcat tccaaacgtt cgtataattg ctgttccaat accgccttga
    82681 tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc taccttgacg attggctggt
    82741 gaagcaagag aaataagtgc atcttttcca gtgatttgct gttttacaac gatagcatcc
    82801 cccaccgcat aaatatcagg aatattcgtt tgataacgtt cgtctactag gatacccccg
    82861 cgtatgccta attcgattcc agcggtcact gctagctggt tatcaggacg aacaccaatg
    82921 gataaaataa ctaaatcagc agggatggta cgcccatcat ccagtcgcaa tcgtttcccg
    82981 tggtcttcaa atccagcgac agcatgagaa gtaatcactt ctactccttg atgagacaat
    83041 tctttttcta taaaagctgc catttcttca tccagtgttg gaagaatatg tggtgcttgt
    83101 tcgatcacca tgacttctaa acctcttctt tttaggtttt ctgccatttc caatccgatg
    83161 aatcctgcac caacgatcac ggcacgcttt ggctgttttt ctaatgcatg catcacttta
    83221 tcgatatctg gtacatttct aatagaaaaa acattgtcgg cttctgttat ccctggaatc
    83281 gatggaacaa ctggttttgc accaggagat aaaaccaatg catcgtactg ttcaatgtgt
    83341 ttttgaccct catgtacgac ttctaccgtt ttgttttcgg gaaagatggc tgtcacttca
    83401 tgatttggac gcacatctaa acaaaaacga gcggataatg attctggcgt ttggacaaga
    83461 agattttccc gttcactgat ttctcccgaa agataataag gcaagccgca atttgcaaaa
    83521 gaaacatacg gtcctttttc aaaaacaacg atttctgcat cttccattaa tcggcgaagc
    83581 cgtgtagcag cagacattcc acctgctaca cctccgacaa taacgatttt catcttattt
    83641 ccctcctact gttcgatcaa accattgatt catgccacca cgtacattca ctacatcata
    83701 accagatttc tttaattctt tagctgcttg tttactcctc atccctgatt gacaaatcac
    83761 atagacggtc ttttctttgt ctccatcata tctgtttata ctttgtaatg gaacattttt
    83821 agctcctttt atatgcccgc cccggtattc tgcaggtgtt ctaacatcaa gtaacgctgg
    83881 attttccaaa agcttttcct ttaattctgg gactgttatc gattttattg attgaaatag
    83941 caaattcatt ttctgcacct catttacatt tttataaata cctcatgggg tatattaaac
    84001 aggagcttta ttaatatgtc aaccagaatg actcttcatt tttttcacta ctttttatat
    84061 ttgcataaaa taccgataag gggtattatc taattataaa aatagaggag aatgtgttat
    84121 ggagattgat aataaaaata tcctgaaccg tttgaaacgt acagagggac aaattcgtgg
    84181 tatccaaaaa atgatcgaag acgaaaaaga atgtatggat gtcatcactc aattaagcgc
    84241 tgttcgttct agtattgatc gcgtaatggg catgatcgtt gcagataatc tgaaaaattg
    84301 ctttgagaaa cctgatagta atccagaaga acaagcggct aaactagaac aagcgatcaa
    84361 gatgattatt aaaaagtaaa ggaatgattt caatgaaagt cgttttccat attgatgaat
    84421 tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct gataaaagct tctccagaaa
    84481 cgacgattgt cgtatctgta aatggcatcg cgattaccgg ttatctagac tctgccaatg
    84541 cagaattctt agatttgcaa ggagtcgttt ttcacgcttg cgccaatgct atgcgtgcta
    84601 atcatatttc agaaagttct ttacctgaac aagtaatcgt tgtaccagca ggtgtccttg
    84661 atttagttga actgcaatcc caaggttatg cttatatcaa gccttagtca gtagaggttg
    84721 cgacagaagt gatcagcttc aagaaatttc ggcttatttt tcgaagaagc tacttctgga
    84781 aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc aagtaataag aacagccaaa
    84841 caaaaatagc ttctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    84901 gaacaccgtt taaggaagag gttgtgaaat cagcggtttg acctaagtat tagagaaaaa
    84961 aacgggaaaa tagctcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
    85021 ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt agtttcgagt attaagaaaa
    85081 attttgtgaa aatggatttc tacatttttg cataaatttg gcttaatacc gagaaactgg
    85141 cttttgaata ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg agtattagag
    85201 aaacaaacgg aaaaatagct gctcatattt tgccgttttt tgagctaata ccgaaggtca
    85261 gctgattgaa caccgtttac caagaggttg tgagcctacc atttaactcc gaatcacaag
    85321 aaataatttc aaaaacaact tctcctgttt ttggttcggt tggcttatta tcgagaagta
    85381 gctactggga gtctatttat caaaaaaagg aatgcgacat aggtcacacc ccttttttat
    85441 tttctttgaa acatctttcg gtactcgaca gcatacaaga caaaagattg taaaagaccg
    85501 taagtgatga cggcaaaaat gactctcacc cacagcgttg gtaattctgg gaaaagcagc
    85561 aaaaggatca tgccaacata gatgacagca gtcgctactt tgccatacca cttcgctcca
    85621 cttaatttga tatttttaga tagcccgatt aatccagcta caaacataaa gccttctttt
    85681 atcaagaata gaccaaacaa cagccaaagc cacggtcgat accatgccat agacaaaatc
    85741 agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca ccttccctaa gtctgttacc
    85801 tgattatatc tccgagcaat gaccccgtct agataatcag acaaacctga gacaacaatg
    85861 accgctcctg ctatataaaa tgactgtctc agcacaaaat aaagataaat cggcagcaaa
    85921 aaaatacgga atatcgataa aagattcgga atcgtttgta tttctttacg ccagtctgtg
    85981 cttaatttca ttttatcgcc ctacctcctc agcactcttt tctttttatt ttagcttaca
    86041 cggggaataa aaccaatgat ttcactgttt ttatttttct ttacagacac tgatattatg
    86101 agctaatttg agataatcac ttgctgtttt catcgtttac tcttctttct tcctgtcgtt
    86161 tgcgcatttc ttttactagt actttatgag ctttttttga tcctcgctct aggtcacgct
    86221 gccgtttacg tgttttataa tcgataaaca gactgtctaa atcgtaacct gtcgggtaca
    86281 gatcttctgc tgaacgcacc agacgaacac gctgacgagg cacgtggatt atttttttgt
    86341 ctaaatatac atcaatcgta tcttcaccta tatcttcata aactaacgct tctttttggt
    86401 attcattgac aaatatccga tcaccttttg caaagagctg acgttctttt ttattctcct
    86461 tcctatttgt aactgctgtc ttaggaagaa attctttttt ctgtgtccga taggcttttt
    86521 ccgccaagta acgctctgct tgatggatca atttcatcga catatccatt ttatgagcaa
    86581 tccacaaagc ttggctttct cctgtttctt ccaacccgca actgatattt aggacgaagt
    86641 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
    86701 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
    86761 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
    86821 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
    86881 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
    86941 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
    87001 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
    87061 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
    87121 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
    87181 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
    87241 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
    87301 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
    87361 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
    87421 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
    87481 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
    87541 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
    87601 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
    87661 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
    87721 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
    87781 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
    87841 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
    87901 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
    87961 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
    88021 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
    88081 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
    88141 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
    88201 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
    88261 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
    88321 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
    88381 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
    88441 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
    88501 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
    88561 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
    88621 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
    88681 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
    88741 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga
    88801 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
    88861 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
    88921 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
    88981 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
    89041 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
    89101 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
    89161 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
    89221 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
    89281 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
    89341 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
    89401 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
    89461 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
    89521 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
    89581 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
    89641 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
    89701 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
    89761 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
    89821 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
    89881 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
    89941 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
    90001 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
    90061 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
    90121 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
    90181 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
    90241 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
    90301 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
    90361 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
    90421 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
    90481 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
    90541 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
    90601 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
    90661 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
    90721 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
    90781 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
    90841 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
    90901 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
    90961 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
    91021 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
    91081 gccgttgttt ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
    91141 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
    91201 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
    91261 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
    91321 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
    91381 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
    91441 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
    91501 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
    91561 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
    91621 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
    91681 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
    91741 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
    91801 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
    91861 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
    91921 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
    91981 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
    92041 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
    92101 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
    92161 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
    92221 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
    92281 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
    92341 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
    92401 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
    92461 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
    92521 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
    92581 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
    92641 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
    92701 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
    92761 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
    92821 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
    92881 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
    92941 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
    93001 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
    93061 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
    93121 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
    93181 tctactatcg aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt
    93241 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
    93301 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
    93361 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
    93421 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
    93481 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
    93541 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
    93601 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
    93661 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
    93721 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
    93781 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
    93841 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
    93901 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
    93961 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
    94021 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
    94081 ttcaaacgtc catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc
    94141 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
    94201 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
    94261 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
    94321 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
    94381 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
    94441 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
    94501 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
    94561 ccacatctga ttgtcgggac gagaatgatt agccagcaaa acaagatcat agttctcctt
    94621 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
    94681 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
    94741 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
    94801 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
    94861 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
    94921 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
    94981 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
    95041 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
    95101 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
    95161 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
    95221 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
    95281 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
    95341 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
    95401 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    95461 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    95521 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    95581 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    95641 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    95701 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    95761 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    95821 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    95881 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    95941 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    96001 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    96061 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    96121 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    96181 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    96241 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    96301 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    96361 tggcaaataa agctctttat atgctttttc aaaaggtttc gttacaaatt catcctcacg
    96421 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    96481 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    96541 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    96601 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    96661 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    96721 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    96781 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    96841 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    96901 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    96961 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    97021 cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    97081 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    97141 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    97201 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    97261 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    97321 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    97381 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    97441 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    97501 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    97561 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    97621 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    97681 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    97741 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    97801 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    97861 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    97921 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    97981 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    98041 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    98101 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    98161 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    98221 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    98281 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    98341 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    98401 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    98461 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    98521 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    98581 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    98641 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    98701 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    98761 tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    98821 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    98881 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    98941 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    99001 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    99061 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    99121 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    99181 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    99241 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    99301 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    99361 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    99421 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    99481 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    99541 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    99601 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    99661 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    99721 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    99781 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    99841 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    99901 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    99961 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
   100021 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
   100081 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
   100141 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
   100201 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
   100261 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga
   100321 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
   100381 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
   100441 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
   100501 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
   100561 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
   100621 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
   100681 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
   100741 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
   100801 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
   100861 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
   100921 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
   100981 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaaataattg
   101041 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
   101101 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
   101161 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
   101221 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
   101281 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
   101341 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
   101401 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
   101461 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
   101521 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
   101581 catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta
   101641 cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta ttatctttta
   101701 gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga
   101761 caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa
   101821 ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca
   101881 ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt
   101941 agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa
   102001 aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct
   102061 gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg
   102121 catttaacgg aagcataact aggtcagaac taggagagat tagttgctga atggaatcta
   102181 tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt
   102241 tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt
   102301 caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt
   102361 gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat
   102421 acactggttc tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt
   102481 ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc
   102541 gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg
   102601 gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac
   102661 gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt
   102721 tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa
   102781 gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt
   102841 ttttttcatc attaatcatc aaataattta actctaatag tcgattttca attgcttgtt
   102901 tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta
   102961 ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa
   103021 tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat
   103081 atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg
   103141 tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata
   103201 agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt
   103261 ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata
   103321 cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt
   103381 gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat
   103441 gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat
   103501 aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt
   103561 tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg
   103621 ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt
   103681 ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat
   103741 agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca
   103801 ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt
   103861 ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct catttcccct
   103921 gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca
   103981 tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga
   104041 tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg
   104101 aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga
   104161 tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca
   104221 tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa
   104281 taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac
   104341 attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct
   104401 cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca
   104461 ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc
   104521 atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac
   104581 atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta
   104641 tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg
   104701 atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc
   104761 gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc
   104821 aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt
   104881 aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct
   104941 cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc
   105001 ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga
   105061 taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata
   105121 ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct
   105181 tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct
   105241 ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt
   105301 atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat
   105361 tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa
   105421 agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc
   105481 ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat
   105541 aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca tattacaaat
   105601 aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt
   105661 ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa
   105721 tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc
   105781 tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg
   105841 ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga ttgattccaa
   105901 gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat
   105961 atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg
   106021 atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc
   106081 ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt
   106141 ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt
   106201 tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc
   106261 taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata
   106321 aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc
   106381 accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt
   106441 gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat
   106501 acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt
   106561 ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc
   106621 atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt
   106681 gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc
   106741 aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat
   106801 atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc
   106861 catgattgct tgataattac tgatatcata cccattgtca tcattcggag actcgtatac
   106921 tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat
   106981 cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg
   107041 ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt
   107101 ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca
   107161 aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt
   107221 ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt
   107281 ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat
   107341 aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc
   107401 tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat
   107461 gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa
   107521 acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa
   107581 tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa
   107641 tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg
   107701 agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg
   107761 tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa
   107821 taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat
   107881 cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc
   107941 aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc
   108001 ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat
   108061 ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc
   108121 gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa
   108181 aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt
   108241 cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc
   108301 agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa
   108361 gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca
   108421 tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg
   108481 tgccgttcca attggataac gggctaatac ccccattaaa acacagccga tcgcactggc
   108541 taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg
   108601 atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg
   108661 ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt
   108721 ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa
   108781 aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta
   108841 agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca
   108901 ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa
   108961 atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg
   109021 tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc
   109081 cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg
   109141 ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt
   109201 tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa
   109261 aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg
   109321 tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct
   109381 ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg
   109441 ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca
   109501 attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt
   109561 gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact
   109621 gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa
   109681 agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaacggcaa
   109741 tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc atctttggtt
   109801 agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt caacgcttcg
   109861 attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac cagcaccgct
   109921 gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag acagcgacag
   109981 atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc atcggaatct
   110041 gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag atttcctgcg
   110101 atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat actattatag
   110161 atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg tttcagtaaa
   110221 gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc ctgacgccac
   110281 tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct tgcttctttt
   110341 gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac gtggttatcg
   110401 ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca gaaattggta
   110461 tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc ttgatgaata
   110521 gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat ttgataaatc
   110581 cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg agccaaatgg
   110641 ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat gtaccgatac
   110701 cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa tgatcagcca
   110761 tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc tcttgatcga
   110821 tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt ttttctagtg
   110881 cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt ccaaatgtat
   110941 ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc gtcatttttc
   111001 gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg ccttttttga
   111061 caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat tccaattgtt
   111121 ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg ttttctcgaa
   111181 tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg atttttttat
   111241 tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg attgctcggg
   111301 cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg attccaggat
   111361 tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact tctttgaatt
   111421 cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga aggatttcag
   111481 ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt gttgcaaatg
   111541 caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt atattctttt
   111601 cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta aaataagtaa
   111661 ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac taaaaaatcc
   111721 catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga tcgcacaagc
   111781 aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat tcgtatcaaa
   111841 aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga cttctttacg
   111901 cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg accattcttt
   111961 cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg cacgaacatc
   112021 tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt cttcgaaagt
   112081 ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct caaaacgctc
   112141 tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga gtagtctttt
   112201 gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg tatgactatg
   112261 taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa tctgcaataa
   112321 ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg ggctgtgaat
   112381 aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa aaatatgacg
   112441 agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc acaacctccc
   112501 cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc atcaaacatg
   112561 agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag gctcacgacc
   112621 tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct ttgttactta
   112681 ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac ttgagtatag
   112741 tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt tcggtcggcg
   112801 atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg atcttccaat
   112861 acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga ttcacgatca
   112921 cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat cgtgatcacc
   112981 tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc actaaaagga
   113041 tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc tttgtaatag
   113101 aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt aaacccgatg
   113161 gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc tggaaatcgt
   113221 tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc cagaagaatg
   113281 aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt tcataaacag
   113341 tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca cattatggcg
   113401 aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg tccagttgac
   113461 taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt caggataatc
   113521 ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta ctgcatcgat
   113581 ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat gattcgtacc
   113641 gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg cgtcttccgg
   113701 tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac cgccgattgt
   113761 tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta ctgcttttgt
   113821 cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag aagtatatcc
   113881 gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt gcaagactgc
   113941 atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat gcatgccgat
   114001 atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt cttttgctgc
   114061 gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa ctgtaaaatt
   114121 caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt attgccacct
   114181 tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc atctcatcca
   114241 ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca tctgcacgct
   114301 tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt ttattttcca
   114361 aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa aatttaagtc
   114421 caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa atctcttgtt
   114481 tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg gctttttctt
   114541 cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca aacatcattt
   114601 tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca tataagagtg
   114661 ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc gaattaaaga
   114721 tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata ccgccaatga
   114781 tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct ccggcaaaga
   114841 aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa gtaaacatgg
   114901 caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt gggctataag
   114961 gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc atagctatcc
   115021 ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc aatacataca
   115081 cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt tgatctcctg
   115141 ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat agcgggacca
   115201 tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc ggcaacttga
   115261 tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca acaccggctt
   115321 caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat ccagtgattt
   115381 tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct ccgataaaac
   115441 caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag actgtcgtca
   115501 tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat tttgtgaaac
   115561 gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata tctctaccta
   115621 aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg atcccttgca
   115681 tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg atgatgctcg
   115741 aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag atgacttgtc
   115801 ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg gtgatcgatg
   115861 cctgagtcaa aatattttcg atgataaaat tgataacgcc catgttttga tgtgcacccc
   115921 aaaagtta
//
LOCUS       DACDIP010000002       103804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDIP010000002 DACDIP010000000
VERSION     DACDIP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 103804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 103804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.002"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IV257_000102"
     CDS             212..604
                     /locus_tag="IV257_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8813264.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IV257_000103"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IV257_000103"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8813265.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IV257_000104"
     CDS             3165..4775
                     /locus_tag="IV257_000104"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8813266.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IV257_000105"
     CDS             5067..5936
                     /locus_tag="IV257_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8813267.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IV257_000106"
     CDS             6101..7372
                     /locus_tag="IV257_000106"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8813268.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IV257_000107"
     CDS             7369..8664
                     /locus_tag="IV257_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8813269.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IV257_000108"
     CDS             8763..9026
                     /locus_tag="IV257_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8813270.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IV257_000109"
     CDS             9471..9920
                     /locus_tag="IV257_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8813271.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IV257_000110"
     CDS             9977..11395
                     /locus_tag="IV257_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8813272.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IV257_000111"
     CDS             11395..12255
                     /locus_tag="IV257_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8813273.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IV257_000112"
     CDS             12429..13202
                     /locus_tag="IV257_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8813274.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IV257_000113"
     CDS             13349..13714
                     /locus_tag="IV257_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8813275.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IV257_000114"
     CDS             complement(13862..15070)
                     /locus_tag="IV257_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8813276.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IV257_000115"
     CDS             complement(15089..15367)
                     /locus_tag="IV257_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813277.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IV257_000116"
     CDS             15560..16216
                     /locus_tag="IV257_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8813278.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IV257_000117"
     CDS             complement(16658..17455)
                     /locus_tag="IV257_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8813279.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IV257_000118"
     CDS             complement(17452..18636)
                     /locus_tag="IV257_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8813280.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IV257_000119"
     CDS             complement(18661..19908)
                     /locus_tag="IV257_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8813281.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IV257_000120"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IV257_000120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8813282.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IV257_000121"
     CDS             21888..22202
                     /locus_tag="IV257_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8813283.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IV257_000122"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IV257_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8813284.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IV257_000123"
     CDS             22887..23531
                     /locus_tag="IV257_000123"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8813285.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IV257_000124"
     CDS             23546..23875
                     /locus_tag="IV257_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813286.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IV257_000125"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IV257_000125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8813287.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IV257_000126"
     CDS             24863..25687
                     /locus_tag="IV257_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8813288.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IV257_000127"
     CDS             25680..26027
                     /locus_tag="IV257_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8813289.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IV257_000128"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IV257_000128"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8813290.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IV257_000129"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IV257_000129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8813291.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IV257_000130"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IV257_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8813292.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IV257_000131"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IV257_000131"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8813293.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IV257_000132"
     CDS             31684..32451
                     /locus_tag="IV257_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813294.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IV257_000133"
     CDS             32441..34483
                     /locus_tag="IV257_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813295.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IV257_000134"
     CDS             34764..34913
                     /locus_tag="IV257_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8813296.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IV257_000135"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IV257_000135"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8813297.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IV257_000136"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IV257_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8813298.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IV257_000137"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IV257_000137"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8813299.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IV257_000138"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IV257_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8813300.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IV257_000139"
     CDS             39330..40412
                     /locus_tag="IV257_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8813301.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IV257_000140"
     CDS             40412..41215
                     /locus_tag="IV257_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8813302.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IV257_000141"
     CDS             41285..42025
                     /locus_tag="IV257_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8813303.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IV257_000142"
     CDS             42036..43040
                     /locus_tag="IV257_000142"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8813304.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="IV257_000143"
     CDS             complement(43085..44929)
                     /locus_tag="IV257_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813305.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="IV257_000144"
     CDS             45141..45659
                     /locus_tag="IV257_000144"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8813306.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="IV257_000145"
     CDS             45787..46470
                     /locus_tag="IV257_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8813307.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="IV257_000146"
     tRNA            46779..46852
                     /locus_tag="IV257_000146"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="IV257_000147"
     CDS             47011..47370
                     /locus_tag="IV257_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8813308.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="IV257_000148"
     CDS             47493..49124
                     /locus_tag="IV257_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8813309.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="IV257_000149"
     CDS             complement(49186..49827)
                     /locus_tag="IV257_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8813310.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="IV257_000150"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="IV257_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8813311.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="IV257_000151"
     CDS             50444..50788
                     /locus_tag="IV257_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8813312.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="IV257_000152"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="IV257_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8813313.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51501..54335
                     /locus_tag="IV257_000153"
     CDS             51501..54335
                     /locus_tag="IV257_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8813314.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="IV257_000154"
     CDS             54526..55017
                     /locus_tag="IV257_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8813315.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="IV257_000155"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="IV257_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8813316.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="IV257_000156"
     CDS             56142..56945
                     /locus_tag="IV257_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8813317.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="IV257_000157"
     CDS             56966..57880
                     /locus_tag="IV257_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8813318.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="IV257_000158"
     CDS             57963..58337
                     /locus_tag="IV257_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8813319.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="IV257_000159"
     CDS             58476..58721
                     /locus_tag="IV257_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8813320.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="IV257_000160"
     CDS             58893..59957
                     /locus_tag="IV257_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8813321.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="IV257_000161"
     CDS             60203..60628
                     /locus_tag="IV257_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8813322.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..61070
                     /gene="nusB"
                     /locus_tag="IV257_000162"
     CDS             60621..61070
                     /gene="nusB"
                     /locus_tag="IV257_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8813323.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61167..62021
                     /locus_tag="IV257_000163"
     CDS             61167..62021
                     /locus_tag="IV257_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8813324.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62022..63359
                     /locus_tag="IV257_000164"
     CDS             62022..63359
                     /locus_tag="IV257_000164"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8813325.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63364..63588
                     /locus_tag="IV257_000165"
     CDS             63364..63588
                     /locus_tag="IV257_000165"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8813326.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63590..64480
                     /locus_tag="IV257_000166"
     CDS             63590..64480
                     /locus_tag="IV257_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8813327.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64489..65307
                     /locus_tag="IV257_000167"
     CDS             64489..65307
                     /locus_tag="IV257_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8813328.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65414..65863
                     /locus_tag="IV257_000168"
     CDS             65414..65863
                     /locus_tag="IV257_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8813329.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65881..67563
                     /gene="recN"
                     /locus_tag="IV257_000169"
     CDS             65881..67563
                     /gene="recN"
                     /locus_tag="IV257_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8813330.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67618..67734)
                     /locus_tag="IV257_000170"
     CDS             complement(67618..67734)
                     /locus_tag="IV257_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8813331.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67807..68763)
                     /locus_tag="IV257_000171"
     CDS             complement(67807..68763)
                     /locus_tag="IV257_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8813332.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68934..69302
                     /locus_tag="IV257_000172"
     CDS             68934..69302
                     /locus_tag="IV257_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8813333.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69472..69903
                     /gene="mraZ"
                     /locus_tag="IV257_000173"
     CDS             69472..69903
                     /gene="mraZ"
                     /locus_tag="IV257_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8813334.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69924..70883
                     /gene="rsmH"
                     /locus_tag="IV257_000174"
     CDS             69924..70883
                     /gene="rsmH"
                     /locus_tag="IV257_000174"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8813335.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70888..71289
                     /gene="ftsL"
                     /locus_tag="IV257_000175"
     CDS             70888..71289
                     /gene="ftsL"
                     /locus_tag="IV257_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8813336.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71290..73482
                     /locus_tag="IV257_000176"
     CDS             71290..73482
                     /locus_tag="IV257_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8813337.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73508..74470
                     /locus_tag="IV257_000177"
     CDS             73508..74470
                     /locus_tag="IV257_000177"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8813338.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74484..75845
                     /locus_tag="IV257_000178"
     CDS             74484..75845
                     /locus_tag="IV257_000178"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8813339.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75871..76959
                     /gene="murG"
                     /locus_tag="IV257_000179"
     CDS             75871..76959
                     /gene="murG"
                     /locus_tag="IV257_000179"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8813340.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76974..78194
                     /locus_tag="IV257_000180"
     CDS             76974..78194
                     /locus_tag="IV257_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8813341.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78350..79675
                     /gene="ftsA"
                     /locus_tag="IV257_000181"
     CDS             78350..79675
                     /gene="ftsA"
                     /locus_tag="IV257_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8813342.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79698..80939
                     /gene="ftsZ"
                     /locus_tag="IV257_000182"
     CDS             79698..80939
                     /gene="ftsZ"
                     /locus_tag="IV257_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8813343.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80956..81633
                     /locus_tag="IV257_000183"
     CDS             80956..81633
                     /locus_tag="IV257_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8813344.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81647..82246
                     /locus_tag="IV257_000184"
     CDS             81647..82246
                     /locus_tag="IV257_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8813345.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82261..82548
                     /locus_tag="IV257_000185"
     CDS             82261..82548
                     /locus_tag="IV257_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8813346.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82597..83379
                     /locus_tag="IV257_000186"
     CDS             82597..83379
                     /locus_tag="IV257_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8813347.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83400..84107
                     /locus_tag="IV257_000187"
     CDS             83400..84107
                     /locus_tag="IV257_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8813348.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84369..87167
                     /gene="ileS"
                     /locus_tag="IV257_000188"
     CDS             84369..87167
                     /gene="ileS"
                     /locus_tag="IV257_000188"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8813349.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87216..88742)
                     /locus_tag="IV257_000189"
     CDS             complement(87216..88742)
                     /locus_tag="IV257_000189"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8813350.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88757..89404)
                     /locus_tag="IV257_000190"
     CDS             complement(88757..89404)
                     /locus_tag="IV257_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8813351.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89588..89917)
                     /locus_tag="IV257_000191"
     CDS             complement(89588..89917)
                     /locus_tag="IV257_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8813352.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90094..90822
                     /gene="sfsA"
                     /locus_tag="IV257_000192"
     CDS             90094..90822
                     /gene="sfsA"
                     /locus_tag="IV257_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8813353.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90838..91851
                     /locus_tag="IV257_000193"
     CDS             90838..91851
                     /locus_tag="IV257_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8813354.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91851..93128
                     /locus_tag="IV257_000194"
     CDS             91851..93128
                     /locus_tag="IV257_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8813355.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93191..95893
                     /locus_tag="IV257_000195"
     CDS             93191..95893
                     /locus_tag="IV257_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8813356.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96045..96362
                     /locus_tag="IV257_000196"
     CDS             96045..96362
                     /locus_tag="IV257_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8813357.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96392..96712
                     /locus_tag="IV257_000197"
     CDS             96392..96712
                     /locus_tag="IV257_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8813358.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96820..98280
                     /locus_tag="IV257_000198"
     CDS             96820..98280
                     /locus_tag="IV257_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8813359.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98348..98569
                     /locus_tag="IV257_000199"
     CDS             98348..98569
                     /locus_tag="IV257_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813360.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98600..98782
                     /locus_tag="IV257_000200"
     CDS             98600..98782
                     /locus_tag="IV257_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8813361.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98782..99195
                     /locus_tag="IV257_000201"
     CDS             98782..99195
                     /locus_tag="IV257_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8813362.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99318..100499
                     /locus_tag="IV257_000202"
     CDS             99318..100499
                     /locus_tag="IV257_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8813363.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            100888..100961
                     /locus_tag="IV257_000203"
     tRNA            100888..100961
                     /locus_tag="IV257_000203"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100922..100924,aa:Arg,seq:cct)
     gene            complement(101030..102169)
                     /locus_tag="IV257_000204"
     CDS             complement(101030..102169)
                     /locus_tag="IV257_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8813364.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(102468..103103)
                     /locus_tag="IV257_000205"
     CDS             complement(102468..103103)
                     /locus_tag="IV257_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813365.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(103216..>103804)
                     /locus_tag="IV257_000206"
     CDS             complement(103216..>103804)
                     /locus_tag="IV257_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP8813366.1"
                     /translation="LIMLIVRFIKKTNKKTPMILLIIGIIFTTVGFSLSSSNEANDNN
                     KQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKY
                     ISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFT
                     KGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        33800 a        17759 c        21859 g        30386 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtag aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    61081 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    61141 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    61201 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    61261 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    61321 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    61381 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    61441 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    61501 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    61561 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    61621 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    61681 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    61741 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    61801 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    61861 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    61921 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    61981 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    62041 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    62101 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    62161 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    62221 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    62281 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    62341 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    62401 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    62461 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    62521 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    62581 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    62641 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    62701 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    62761 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    62821 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    62881 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    62941 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    63001 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    63061 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    63121 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    63181 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    63241 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    63301 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    63361 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    63421 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    63481 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    63541 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    63601 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    63661 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    63721 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    63781 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    63841 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    63901 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    63961 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    64021 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    64081 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    64141 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    64201 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    64261 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    64321 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    64381 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    64441 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    64501 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    64561 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    64621 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    64681 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    64741 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    64801 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    64861 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    64921 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    64981 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    65041 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    65101 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    65161 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    65221 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    65281 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    65341 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    65401 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    65461 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    65521 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    65581 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    65641 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    65701 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    65761 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    65821 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    65881 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    65941 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    66001 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    66061 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    66121 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    66181 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    66241 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    66301 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    66361 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    66421 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    66481 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    66541 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    66601 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    66661 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    66721 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    66781 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    66841 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    66901 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    66961 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    67021 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    67081 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    67141 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    67201 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    67261 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    67321 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    67381 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    67441 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    67501 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    67561 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    67621 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    67681 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    67741 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    67801 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    67861 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    67921 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    67981 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    68041 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    68101 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    68161 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    68221 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    68281 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    68341 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    68401 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    68461 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    68521 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    68581 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    68641 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    68701 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    68761 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    68821 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    68881 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    68941 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    69001 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    69061 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    69121 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    69181 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    69241 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    69301 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    69361 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    69421 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    69481 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    69541 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    69601 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    69661 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    69721 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    69781 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    69841 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    69901 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    69961 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    70021 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    70081 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    70141 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    70201 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    70261 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    70321 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    70381 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    70441 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    70501 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    70561 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    70621 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    70681 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    70741 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    70801 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    70861 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    70921 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    70981 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    71041 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    71101 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    71161 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    71221 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    71281 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    71341 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggaatgttc
    71401 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    71461 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    71521 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    71581 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    71641 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    71701 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    71761 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    71821 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    71881 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    71941 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    72001 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    72061 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    72121 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    72181 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    72241 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    72301 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    72361 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    72421 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    72481 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    72541 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    72601 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    72661 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    72721 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    72781 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    72841 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaacc
    72901 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    72961 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    73021 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    73081 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    73141 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    73201 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    73261 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    73321 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    73381 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    73441 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    73501 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    73561 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    73621 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    73681 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    73741 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    73801 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    73861 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    73921 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    73981 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    74041 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    74101 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    74161 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    74221 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    74281 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    74341 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    74401 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    74461 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    74521 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    74581 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    74641 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    74701 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    74761 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    74821 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    74881 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    74941 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    75001 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    75061 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    75121 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    75181 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    75241 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    75301 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    75361 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    75421 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    75481 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    75541 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    75601 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    75661 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    75721 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    75781 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    75841 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    75901 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    75961 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    76021 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    76081 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    76141 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    76201 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    76261 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    76321 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    76381 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    76441 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    76501 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    76561 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    76621 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    76681 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    76741 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    76801 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    76861 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    76921 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    76981 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    77041 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    77101 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    77161 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    77221 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    77281 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    77341 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    77401 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    77461 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    77521 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    77581 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    77641 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    77701 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    77761 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    77821 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    77881 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    77941 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    78001 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    78061 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    78121 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    78181 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    78241 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    78301 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    78361 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    78421 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    78481 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    78541 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    78601 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    78661 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    78721 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    78781 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    78841 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    78901 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    78961 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    79021 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    79081 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    79141 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    79201 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    79261 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    79321 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    79381 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    79441 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    79501 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    79561 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    79621 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    79681 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    79741 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    79801 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    79861 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    79921 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    79981 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    80041 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    80101 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    80161 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    80221 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    80281 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    80341 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    80401 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    80461 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    80521 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    80581 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    80641 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    80701 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    80761 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    80821 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    80881 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    80941 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    81001 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    81061 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    81121 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    81181 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    81241 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    81301 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    81361 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    81421 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    81481 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    81541 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    81601 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    81661 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    81721 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    81781 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    81841 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    81901 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    81961 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    82021 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    82081 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    82141 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    82201 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    82261 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    82321 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    82381 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    82441 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    82501 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    82561 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    82621 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    82681 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    82741 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    82801 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    82861 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    82921 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    82981 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    83041 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    83101 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    83161 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    83221 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    83281 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    83341 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    83401 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    83461 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    83521 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    83581 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    83641 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    83701 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    83761 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    83821 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    83881 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    83941 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    84001 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    84061 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    84121 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    84181 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    84241 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    84301 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    84361 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    84421 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    84481 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    84541 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    84601 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    84661 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    84721 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    84781 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    84841 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaagc tatatttaca
    84901 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    84961 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    85021 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    85081 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    85141 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    85201 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    85261 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    85321 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    85381 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    85441 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    85501 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    85561 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    85621 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    85681 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    85741 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    85801 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    85861 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    85921 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    85981 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    86041 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    86101 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    86161 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    86221 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    86281 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    86341 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    86401 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    86461 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    86521 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    86581 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    86641 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    86701 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    86761 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    86821 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    86881 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    86941 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    87001 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    87061 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    87121 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    87181 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    87241 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    87301 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    87361 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    87421 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    87481 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    87541 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    87601 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    87661 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    87721 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    87781 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    87841 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    87901 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    87961 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    88021 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    88081 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    88141 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    88201 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    88261 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    88321 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    88381 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    88441 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    88501 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    88561 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    88621 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    88681 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    88741 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    88801 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    88861 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    88921 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    88981 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    89041 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    89101 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    89161 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    89221 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    89281 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    89341 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    89401 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    89461 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    89521 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    89581 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    89641 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    89701 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    89761 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    89821 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    89881 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    89941 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    90001 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    90061 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    90121 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    90181 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    90241 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    90301 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    90361 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    90421 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    90481 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    90541 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    90601 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    90661 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    90721 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    90781 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    90841 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    90901 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    90961 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    91021 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    91081 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    91141 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    91201 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    91261 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    91321 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    91381 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    91441 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    91501 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    91561 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    91621 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    91681 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    91741 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    91801 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    91861 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    91921 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    91981 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    92041 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    92101 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    92161 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    92221 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    92281 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    92341 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    92401 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    92461 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    92521 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    92581 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    92641 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    92701 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    92761 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    92821 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    92881 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    92941 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    93001 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    93061 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    93121 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    93181 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    93241 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    93301 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    93361 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    93421 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    93481 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    93541 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    93601 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    93661 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    93721 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    93781 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    93841 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    93901 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    93961 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    94021 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    94081 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    94141 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    94201 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    94261 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    94321 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    94381 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    94441 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    94501 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    94561 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    94621 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    94681 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    94741 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    94801 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    94861 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    94921 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    94981 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    95041 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    95101 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    95161 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    95221 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    95281 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    95341 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    95401 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    95461 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    95521 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    95581 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    95641 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    95701 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    95761 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    95821 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    95881 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    95941 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    96001 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    96061 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    96121 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    96181 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    96241 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    96301 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    96361 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    96421 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    96481 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    96541 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    96601 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    96661 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    96721 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    96781 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    96841 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    96901 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    96961 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    97021 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    97081 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    97141 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    97201 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    97261 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    97321 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    97381 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    97441 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    97501 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    97561 gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct
    97621 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    97681 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    97741 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    97801 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    97861 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    97921 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    97981 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    98041 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    98101 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    98161 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    98221 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    98281 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    98341 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    98401 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    98461 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    98521 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    98581 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    98641 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    98701 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    98761 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    98821 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    98881 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    98941 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    99001 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    99061 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    99121 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    99181 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    99241 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    99301 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    99361 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    99421 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    99481 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    99541 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    99601 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    99661 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    99721 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    99781 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    99841 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    99901 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    99961 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
   100021 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
   100081 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
   100141 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
   100201 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
   100261 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
   100321 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
   100381 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
   100441 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
   100501 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
   100561 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
   100621 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
   100681 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
   100741 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
   100801 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
   100861 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
   100921 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc
   100981 ctttgcggta caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat
   101041 ttttatataa actatcgaac acagtattga ttttttcttt atcctgatct tcaagctctc
   101101 gaacaatatg aagataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
   101161 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc
   101221 catgcaaagt aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc
   101281 tgacggcgtt tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt
   101341 ttttgatttc aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat
   101401 caatcgtatg tttatcaatg gaaattttcc tatttgaagt ttcgtttttt gtttttcccc
   101461 attcatttaa tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga
   101521 tatcttccca tgttaatcct agaagttcag caaatcgcat tccagtggca ccagcaacta
   101581 acacgagcat aggagaagag tactgcgcag aaaggttttc ttctacgagt ttcattaaag
   101641 atttaaattg atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt
   101701 tgattactgc ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca
   101761 acgacgctct aatatggtta ttaaacttaa taacggtaga ttttacatgg tctttagcat
   101821 acttatttaa aaagcgttga tatcccactc tatctaaatc agaaatcaat accgctggca
   101881 tatatttctt tatgttcatt aacgtatctt cgtattttct ataagtgata ggagatactg
   101941 ttccttcttt ataaagttgc atccaatcct caaagtaatc tgataataat agatttttgc
   102001 gatccccttt aaggcctttg ttcaagttat attctaattc gttggcggca tcttttgctt
   102061 ctccttttgt cttaaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt
   102121 atgaaatttc gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcag
   102181 ctctctttct ctgatacaat aggaactata aagaagccca ttgtttgggt tttattattt
   102241 tttgcacatc ctttttgctt gccggcgcaa ggatgtgcta tttgtcgttt taatggtata
   102301 attggatgtg tattagatca tccaatcaga ggtgtttatt atgattaaag tgatttttaa
   102361 aagaataact gaacttccag agtttagatt atttgttgat tattggtatg tctggttttt
   102421 tattgttgca ttgctgatag gtctagatgt catattagaa agaaaactta agtaagagtt
   102481 gagttaataa attgaattcc taaatgagat attttgtagc ccatattttg tttgccgaca
   102541 tcacctaata ctatggagct aatcgttttt tctaacagac caaaatcaga taatctattt
   102601 aagctaccat ctattagctc ttgtgaatag ccagacatgc tgatatttcc agtgaaattt
   102661 tcgtttccac gactcattaa gcatgctaac aattgaatct caatcgatgt taattgattc
   102721 agtacaagaa gataatactc ctcgttatcc catgaagcgt tgttaattcg aagtaaacaa
   102781 ttcttataaa gatttttata catatctatt ttattttgtg cctttgattt ttctacctcg
   102841 tcatttatcc cttcgaatat tccaatgagt tggtccttgt tctctaagtt gaaaacatct
   102901 tctggaattt gatcgcgaat gttttctaat gattcgttta aatttttgta gaatttctcg
   102961 atgcgtttga aacgcttctc gtttttagac ccaaaataaa gtgactgcac aacgccacca
   103021 atataaggaa ttgctccaat accgccttca actattaatt caagatattc ttttttggtt
   103081 agtttttcat tgctattatc catagttacc tcctattttg tcatttcctt tacaagcatt
   103141 gtgactcctg atggcactct tttgtatagg ttttattatt gtgcacaatc taactcttag
   103201 tggggcgtgg ttcatttatt ttttacttac tttactatta atcatatccc attgagcata
   103261 ggatttttct atgttcgatc ctcttgaact aatttctcct gtaaaagtta ctttatcgcc
   103321 ctttgtaaag gtatcagcat tgtttaagtc cttcgcaaaa attacatatg aaactttagg
   103381 tgttgcagaa atatcattcc aacttttatc atcgtactct ttgtctgcta taatggctat
   103441 tctagtagtc attgattcga taacagttcc ttcaaaagta tatgttttac cactaatata
   103501 tttagtaaaa gctgtatttt gctcagtagc tggagtaata gaataataac tatctaagaa
   103561 agcaccccag tcatgattat ttgtctctaa aaattcttca aattgagtta ggcttttatt
   103621 gctgctgtta gatgtagtag tttgagtatc agatgatgag ctttcctgtt tgttattgtc
   103681 atttgcctcg tttgatgagg aaagcgagaa acctacggta gtaaaaataa tcccgataat
   103741 aaggagaatc ataggtgttt tcttatttgt ttttttgata aatcttacaa ttaacatgat
   103801 taaa
//
LOCUS       DACDIP010000003       103542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDIP010000003 DACDIP010000000
VERSION     DACDIP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 103542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 103542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.003"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1253)
                     /locus_tag="IV257_000207"
     CDS             complement(<1..1253)
                     /locus_tag="IV257_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8813367.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNE"
     gene            complement(1681..2754)
                     /locus_tag="IV257_000208"
     CDS             complement(1681..2754)
                     /locus_tag="IV257_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8813368.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(2751..3578)
                     /locus_tag="IV257_000209"
     CDS             complement(2751..3578)
                     /locus_tag="IV257_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813369.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(3579..4385)
                     /locus_tag="IV257_000210"
     CDS             complement(3579..4385)
                     /locus_tag="IV257_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813370.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(4397..5482)
                     /locus_tag="IV257_000211"
     CDS             complement(4397..5482)
                     /locus_tag="IV257_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813371.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(5499..6041)
                     /locus_tag="IV257_000212"
     CDS             complement(5499..6041)
                     /locus_tag="IV257_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8813372.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            6299..7288
                     /locus_tag="IV257_000213"
     CDS             6299..7288
                     /locus_tag="IV257_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8813373.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7285..8022
                     /locus_tag="IV257_000214"
     CDS             7285..8022
                     /locus_tag="IV257_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8813374.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            8019..8570
                     /gene="coaC"
                     /locus_tag="IV257_000215"
     CDS             8019..8570
                     /gene="coaC"
                     /locus_tag="IV257_000215"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8813375.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            8586..9218
                     /locus_tag="IV257_000216"
     CDS             8586..9218
                     /locus_tag="IV257_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8813376.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            9303..9950
                     /locus_tag="IV257_000217"
     CDS             9303..9950
                     /locus_tag="IV257_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8813377.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            9955..10938
                     /locus_tag="IV257_000218"
     CDS             9955..10938
                     /locus_tag="IV257_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8813378.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(11341..12855)
                     /locus_tag="IV257_000219"
     CDS             complement(11341..12855)
                     /locus_tag="IV257_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8813379.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            12993..13799
                     /locus_tag="IV257_000220"
     CDS             12993..13799
                     /locus_tag="IV257_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813380.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(13974..14543)
                     /locus_tag="IV257_000221"
     CDS             complement(13974..14543)
                     /locus_tag="IV257_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8813381.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(14626..16686)
                     /locus_tag="IV257_000222"
     CDS             complement(14626..16686)
                     /locus_tag="IV257_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813382.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(16963..18441)
                     /locus_tag="IV257_000223"
     CDS             complement(16963..18441)
                     /locus_tag="IV257_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8813383.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(18739..19674)
                     /gene="murB"
                     /locus_tag="IV257_000224"
     CDS             complement(18739..19674)
                     /gene="murB"
                     /locus_tag="IV257_000224"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8813384.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(19747..20667)
                     /locus_tag="IV257_000225"
     CDS             complement(19747..20667)
                     /locus_tag="IV257_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8813385.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            20780..21532
                     /gene="xth"
                     /locus_tag="IV257_000226"
     CDS             20780..21532
                     /gene="xth"
                     /locus_tag="IV257_000226"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8813386.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            22396..22929
                     /locus_tag="IV257_000227"
     CDS             22396..22929
                     /locus_tag="IV257_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8813387.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(23419..23943)
                     /locus_tag="IV257_000228"
     CDS             complement(23419..23943)
                     /locus_tag="IV257_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8813388.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(23936..24409)
                     /gene="tsaE"
                     /locus_tag="IV257_000229"
     CDS             complement(23936..24409)
                     /gene="tsaE"
                     /locus_tag="IV257_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8813389.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(24532..25512)
                     /gene="pta"
                     /locus_tag="IV257_000230"
     CDS             complement(24532..25512)
                     /gene="pta"
                     /locus_tag="IV257_000230"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8813390.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(25665..26345)
                     /locus_tag="IV257_000231"
     CDS             complement(25665..26345)
                     /locus_tag="IV257_000231"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8813391.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            26471..27340
                     /locus_tag="IV257_000232"
     CDS             26471..27340
                     /locus_tag="IV257_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8813392.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            27366..27881
                     /locus_tag="IV257_000233"
     CDS             27366..27881
                     /locus_tag="IV257_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813393.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(27928..28491)
                     /locus_tag="IV257_000234"
     CDS             complement(27928..28491)
                     /locus_tag="IV257_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8813394.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(28546..28725)
                     /locus_tag="IV257_000235"
     CDS             complement(28546..28725)
                     /locus_tag="IV257_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813395.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(28802..29311)
                     /locus_tag="IV257_000236"
     CDS             complement(28802..29311)
                     /locus_tag="IV257_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813396.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(29407..29787)
                     /locus_tag="IV257_000237"
     CDS             complement(29407..29787)
                     /locus_tag="IV257_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8813397.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            30117..30866
                     /locus_tag="IV257_000238"
     CDS             30117..30866
                     /locus_tag="IV257_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8813398.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(31050..32327)
                     /locus_tag="IV257_000239"
     CDS             complement(31050..32327)
                     /locus_tag="IV257_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813399.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(32406..33050)
                     /gene="pcp"
                     /locus_tag="IV257_000240"
     CDS             complement(32406..33050)
                     /gene="pcp"
                     /locus_tag="IV257_000240"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8813400.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(33249..34193)
                     /locus_tag="IV257_000241"
     CDS             complement(33249..34193)
                     /locus_tag="IV257_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8813401.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(34195..34881)
                     /locus_tag="IV257_000242"
     CDS             complement(34195..34881)
                     /locus_tag="IV257_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8813402.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            35354..35674
                     /locus_tag="IV257_000243"
     CDS             35354..35674
                     /locus_tag="IV257_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8813403.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            35835..36740
                     /locus_tag="IV257_000244"
     CDS             35835..36740
                     /locus_tag="IV257_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8813404.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            36737..37510
                     /locus_tag="IV257_000245"
     CDS             36737..37510
                     /locus_tag="IV257_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8813405.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(37687..39111)
                     /locus_tag="IV257_000246"
     CDS             complement(37687..39111)
                     /locus_tag="IV257_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8813406.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            39322..39768
                     /gene="nrdI"
                     /locus_tag="IV257_000247"
     CDS             39322..39768
                     /gene="nrdI"
                     /locus_tag="IV257_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8813407.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(39838..41820)
                     /locus_tag="IV257_000248"
     CDS             complement(39838..41820)
                     /locus_tag="IV257_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8813408.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(41834..42730)
                     /gene="murQ"
                     /locus_tag="IV257_000249"
     CDS             complement(41834..42730)
                     /gene="murQ"
                     /locus_tag="IV257_000249"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8813409.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(42733..43794)
                     /locus_tag="IV257_000250"
     CDS             complement(42733..43794)
                     /locus_tag="IV257_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8813410.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(44006..44533)
                     /locus_tag="IV257_000251"
     CDS             complement(44006..44533)
                     /locus_tag="IV257_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8813411.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(44600..46102)
                     /gene="lsa"
                     /locus_tag="IV257_000252"
     CDS             complement(44600..46102)
                     /gene="lsa"
                     /locus_tag="IV257_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8813412.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(46360..47481)
                     /locus_tag="IV257_000253"
     CDS             complement(46360..47481)
                     /locus_tag="IV257_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8813413.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            47607..48548
                     /locus_tag="IV257_000254"
     CDS             47607..48548
                     /locus_tag="IV257_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8813414.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(48691..49587)
                     /locus_tag="IV257_000255"
     CDS             complement(48691..49587)
                     /locus_tag="IV257_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8813415.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            49869..51209
                     /locus_tag="IV257_000256"
     CDS             49869..51209
                     /locus_tag="IV257_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8813416.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            51404..51847
                     /locus_tag="IV257_000257"
     CDS             51404..51847
                     /locus_tag="IV257_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8813417.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(52109..53527)
                     /gene="nhaC"
                     /locus_tag="IV257_000258"
     CDS             complement(52109..53527)
                     /gene="nhaC"
                     /locus_tag="IV257_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8813418.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(53931..55154)
                     /locus_tag="IV257_000259"
     CDS             complement(53931..55154)
                     /locus_tag="IV257_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8813419.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(55186..56025)
                     /locus_tag="IV257_000260"
     CDS             complement(55186..56025)
                     /locus_tag="IV257_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8813420.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(56042..56722)
                     /locus_tag="IV257_000261"
     CDS             complement(56042..56722)
                     /locus_tag="IV257_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813421.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(56724..57797)
                     /locus_tag="IV257_000262"
     CDS             complement(56724..57797)
                     /locus_tag="IV257_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8813422.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(58447..59118)
                     /locus_tag="IV257_000263"
     CDS             complement(58447..59118)
                     /locus_tag="IV257_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8813423.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(59206..61884)
                     /gene="mgtA"
                     /locus_tag="IV257_000264"
     CDS             complement(59206..61884)
                     /gene="mgtA"
                     /locus_tag="IV257_000264"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8813424.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWFFITVVAYLALVTAVKKIYIRRFQELL"
     gene            62544..62981
                     /gene="ndk"
                     /locus_tag="IV257_000265"
     CDS             62544..62981
                     /gene="ndk"
                     /locus_tag="IV257_000265"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8813425.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(63003..63869)
                     /locus_tag="IV257_000266"
     CDS             complement(63003..63869)
                     /locus_tag="IV257_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8813426.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(64103..66181)
                     /locus_tag="IV257_000267"
     CDS             complement(64103..66181)
                     /locus_tag="IV257_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8813427.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            66674..67891
                     /locus_tag="IV257_000268"
     CDS             66674..67891
                     /locus_tag="IV257_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8813428.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(68350..68973)
                     /locus_tag="IV257_000269"
     CDS             complement(68350..68973)
                     /locus_tag="IV257_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813429.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(69129..70526)
                     /locus_tag="IV257_000270"
     CDS             complement(69129..70526)
                     /locus_tag="IV257_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8813430.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(70548..71519)
                     /locus_tag="IV257_000271"
     CDS             complement(70548..71519)
                     /locus_tag="IV257_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8813431.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(71526..73826)
                     /locus_tag="IV257_000272"
     CDS             complement(71526..73826)
                     /locus_tag="IV257_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAP8813432.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(73828..75153)
                     /locus_tag="IV257_000273"
     CDS             complement(73828..75153)
                     /locus_tag="IV257_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8813433.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            75384..75809
                     /locus_tag="IV257_000274"
     CDS             75384..75809
                     /locus_tag="IV257_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813434.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(76307..77008)
                     /locus_tag="IV257_000275"
     CDS             complement(76307..77008)
                     /locus_tag="IV257_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8813435.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(77024..77464)
                     /locus_tag="IV257_000276"
     CDS             complement(77024..77464)
                     /locus_tag="IV257_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAP8813436.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(77464..78039)
                     /locus_tag="IV257_000277"
     CDS             complement(77464..78039)
                     /locus_tag="IV257_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8813437.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(78450..78956)
                     /locus_tag="IV257_000278"
     CDS             complement(78450..78956)
                     /locus_tag="IV257_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP8813438.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(78967..79446)
                     /locus_tag="IV257_000279"
                     /pseudo
     CDS             complement(78967..79446)
                     /locus_tag="IV257_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(79618..80319)
                     /locus_tag="IV257_000280"
     CDS             complement(79618..80319)
                     /locus_tag="IV257_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8813439.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(80427..81869)
                     /locus_tag="IV257_000281"
     CDS             complement(80427..81869)
                     /locus_tag="IV257_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8813440.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(81883..83748)
                     /locus_tag="IV257_000282"
     CDS             complement(81883..83748)
                     /locus_tag="IV257_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8813441.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(83869..84720)
                     /locus_tag="IV257_000283"
     CDS             complement(83869..84720)
                     /locus_tag="IV257_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8813442.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(85030..85743)
                     /locus_tag="IV257_000284"
     CDS             complement(85030..85743)
                     /locus_tag="IV257_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8813443.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(85811..86137)
                     /locus_tag="IV257_000285"
     CDS             complement(85811..86137)
                     /locus_tag="IV257_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8813444.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(86260..86487)
                     /locus_tag="IV257_000286"
     CDS             complement(86260..86487)
                     /locus_tag="IV257_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8813445.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(86809..88062)
                     /locus_tag="IV257_000287"
     CDS             complement(86809..88062)
                     /locus_tag="IV257_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8813446.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            88123..88863
                     /locus_tag="IV257_000288"
     CDS             88123..88863
                     /locus_tag="IV257_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8813447.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(88949..89707)
                     /locus_tag="IV257_000289"
     CDS             complement(88949..89707)
                     /locus_tag="IV257_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8813448.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(89704..90732)
                     /locus_tag="IV257_000290"
                     /pseudo
     CDS             complement(89704..90732)
                     /locus_tag="IV257_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(90779..93919)
                     /locus_tag="IV257_000291"
     CDS             complement(90779..93919)
                     /locus_tag="IV257_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813449.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            complement(94143..95027)
                     /locus_tag="IV257_000292"
     CDS             complement(94143..95027)
                     /locus_tag="IV257_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8813450.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(95122..95559)
                     /locus_tag="IV257_000293"
     CDS             complement(95122..95559)
                     /locus_tag="IV257_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813451.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(95776..96687)
                     /locus_tag="IV257_000294"
     CDS             complement(95776..96687)
                     /locus_tag="IV257_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8813452.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(97144..97572)
                     /locus_tag="IV257_000295"
     CDS             complement(97144..97572)
                     /locus_tag="IV257_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813453.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(97600..98238)
                     /locus_tag="IV257_000296"
     CDS             complement(97600..98238)
                     /locus_tag="IV257_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813454.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(98242..100278)
                     /locus_tag="IV257_000297"
     CDS             complement(98242..100278)
                     /locus_tag="IV257_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8813455.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            100404..101069
                     /locus_tag="IV257_000298"
     CDS             100404..101069
                     /locus_tag="IV257_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8813456.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(102560..103432)
                     /locus_tag="IV257_000299"
     CDS             complement(102560..103432)
                     /locus_tag="IV257_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8813457.1"
                     /translation="MIYGAIEAGGTKFICAVSTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQRKSKKYGHITSTPKLAWQNFNFVGTLKQHFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGI
                     IGGLALARKAIRNK"
BASE COUNT        30260 a        21503 c        17789 g        33990 t
ORIGIN      
        1 ttttcattga aattaccttc atttatcagc tgagtaattt tttccaaaat cgaatcataa
       61 tcaaacggtt tgattcgatc cgtttttatc ttttgatttt tctttatcaa taaactagca
      121 atcggcaagc tttctacttc ggattccagc atatttaact gaatcaagat ctcatttttc
      181 ccaatcgtta atggttcttc ttgtgttcga ctatacacat ccgcacaact gattttaatg
      241 atattcttca gcttaccaat attcccacta aagggatgag ataacattaa agacaaggct
      301 tctcttgaaa caataatatc tttttgtaaa acatccgctt cattttgaaa aaataattga
      361 atcagctcta atcgttctcg aatggggcga tcttcaaaag ttggcaacat aacactaatt
      421 tgaatacgcc gatcaaaggt gtctaataaa taattttcac ctttttccgt cgtggcgaaa
      481 ataaaacgaa ccttagcaga atgccaattt acattttcac cgactggacg atagtttcca
      541 gtatcaataa atagaaagag cttttcttga ttttctttcg ataggcgatg gacctcatcg
      601 agaaaaagat agccgttgtt ggccgttgcc aataaaccat cggtatcctt ttcagccccc
      661 gtaaaggccc ccttcttgta tccaaacagt gttgccgaaa gcagttcggg attgtccgca
      721 taatcggcac agtttaagat agcaaatggt gcatcttttt caataatttg ctcatggata
      781 gcgtattcaa aaatttttgt ggctaaaaaa ctttttccaa cacccgtggc tccatttatt
      841 aatacactga gtccgttagg cggatatttg acagcggcta tacatttttg aatgacctct
      901 ctcagacttc cgtcatgacc aactacagaa tgaaaggctt cgtttatttg ctccgtatta
      961 tctacaaccg catattcatc taaaggcttc cacaaaacag gctttcccgg taatttctcg
     1021 acctttttat cttccactaa tctcgtcaaa taatggctgg cattctgacg agagatacct
     1081 agattcttcg aaattttctc ggcagtgaca gaagacatat tccctgttag tcgtatcagc
     1141 tcatcaaaaa ctcgatcttt agcgctcttt ttcatttttt cctccagttt aatgtgtcta
     1201 aaagcataat aaactatcgg ctgcaatatg tctattctat atttgtattc caatttttac
     1261 tttgaaacac aaatagtcga atatttgcct cagatatctg acaaaataat ttatctacca
     1321 atttagtttt tatctaatat gatttttatg ttttttcata gctacaaata ttgaactgga
     1381 ttcatatatt agttttatta atagattaca gaagggttaa gtatatttgc atagtttcca
     1441 attactttat ccttctgtaa ctttttcaat cttagattta ctagcccgtc tcgggcccta
     1501 ttttacaatg gttttgtcca cttttctctg gtagaaaaac gtggtagaaa aaataaaata
     1561 acacaaaaaa agcggcgttt catccgcttt tggtagaaga ttggaaaact cagatagaaa
     1621 aaggacgatt tttagctctt tagaacgttg ataagtcccc attcttactg agttttgcca
     1681 ttatctccta tacatcttga attctaagaa taggtcatta tagatgccta ggtatttcga
     1741 tcctagatct ttgtagactt caagatggga gatcgtgtca tcagatggat agaattgttc
     1801 gtccgacgaa atgtcttttg gcaataacgc ttttgcttct tcatttggtg tagaataacc
     1861 aatatactct gcattttgtg ccgcattttc cggttctagc ataaagttga tgaaatcata
     1921 ggcaccttct ttatttttca cggtttttgg cataacgata ttatcaaacc aaagattgga
     1981 tccttctggt gggatcacat aatgtaaatg ttcattttcc cacatcatat cggcagcttc
     2041 gccagaaaat gtcactgcca cggaggcttc ttcattgatc atatacattt tgatctcatc
     2101 tgccacgatt gctttgacat ttggtgttaa atgattcaat ttatttgctg cttctgtcag
     2161 ctgtgccata ttcttactat tcaaagaata gcctaaacta ttcaaagaaa gccccataac
     2221 ttctcgtgca ccgtcaatca gcatcaaact gtcttttaat tccggcttcc acaaatcatc
     2281 ccaatgctta atttgatttg catcaaagaa tttatcgtta tatacgatcc ctaaagttcc
     2341 ccaaaaatat ggaatcgaat actgattatc tggatcaaaa tccaagtcta aaaatcgtgg
     2401 atcaatatta tttaatcctt tgatcttcga atgatctaat ggaagtacta gtttttcttt
     2461 gatcattttt tggatcatat attcacttgg tatagcgatg tcgtagtttg tcccaccttg
     2521 ctggattttt gtgaacatcg cttcattaga atcaaatgtt tcataattca ctttgtatcc
     2581 atattctttt tcaaatttcg tgatcaaact aggatcgata taatctcccc agttatagat
     2641 ggtaagtacc tttgctccac tcatgccact tgttttttca agttgacgaa caccaagcag
     2701 cagcaccgca ataatgatga gaatacctaa aaacagagat tgtaattttt tcatcgcagc
     2761 cgtgccaccc cctcgcgtac tcttctgttt ttcttcagcc gttttggacg gttttcttta
     2821 ctaataaagt agtatccaat gaccaatatc atggagaaca agaaaactaa cgcacttagc
     2881 gcattgattt ctaaactgat tccttgtctt gcacgagaat agatctctac tgagagcgtc
     2941 gtaaagccat ttcctgtgac gaaaaatgta accgcaaaat catcaagaga ataggtaaat
     3001 gccataaaat atccagctat gattcctgga gaaagataag gcaaaatgat atttttgact
     3061 acttggaaat tattagcacc aaggtctcta gccgcatcga ccattgaatc gttcatttcc
     3121 tgcaattttg gcaagaccat caagacgacg attggaatgc tgaaagcaat atgggaaagc
     3181 aatacactct gaaaacctaa tccgaatcct agtagtgtaa agaaaatcaa gaaactagca
     3241 ccgataataa catcaggaga gactaataag atgttattca tgctcaacaa cgctgtccgt
     3301 gcacgtcgtt ttttcgtgta atagattccc atagccccaa atgtcccgat gatcgtggcg
     3361 atcaatgcag ataagaaagc cagcatgaac gtattcaata cgataatgat caaacgtgta
     3421 tcttcaaaaa cagcttcata attttcccaa gtaaatcctg taaagctgtt catcgttcct
     3481 ccttcattga aggaataaaa aatcagatag aagatcggtg catataagaa taaaaagacg
     3541 atccctaaat aaagatacga ccatttaaat tttttcattt atgagcgacc tccttttttc
     3601 ttctcgcctg tcaggagcat cacgatgaac atagcaatga ttaaaatcac accgattgta
     3661 gaacccatcc cccagttttg tgtaaccaag aaatgctgtt cgatagccgt tcctaaagtg
     3721 atcacccgat ttccgccaat cagacgagtc agcatgaata aagaaagaga aggaataaat
     3781 actgcttgga caccactttt cacaccattt aatgataacg gaaaaatcac tcgtcggaag
     3841 gtctcaaagc tattcgcgcc taaatctcta ctagcactga tcaaagaagg attcatctct
     3901 tccaatgcat tgaaaattgg cataatcata aatggaatct cgatatacgt tgccacgaac
     3961 agaaaactga agtctgtgaa taatatctgt ttcggcccga tcccgataaa atcaaagaaa
     4021 tgattcacgc tgccgtgcac actgaaaatc ccaataaatg cgtaagcttt aagcagcaga
     4081 ttcacccatg taggcaaaat aatcagcatc agccataatt gcttgtgctt cagttttgtt
     4141 aaaaaataag cagtcgggta actgatgatc agcgtgaaaa acgtgatcaa aaatgcatac
     4201 catacagaat tgatcgtcat agaaagcgat gtccccgacg taaaataaga tagataatta
     4261 ttcagcgtga attgcccatt catatcaaaa aaagattgat aaatgatcat taatacaggc
     4321 gcgatcacga ataaaccgag ccacaaaata taagggacgg aatagatcct gcgcattgtt
     4381 ttcattcttg tcccccctat tcttcgtagc tgtcaagacg tgcatcgaat tcttcttcag
     4441 actcgttgaa acgcatgaca tggatatctt ctggctcaaa ggccaatcct actttgcttc
     4501 cttctttagc ttttttcgtt gaatggacca tccattcatt ttgctgttca tcataacaga
     4561 tgatttcata atggacacca cgaaacagct gagtatcgac cgttacgact agttttcctt
     4621 tatctggtgt cgtgatcgtc aaatcctctg ggcgtaaaac aacttcaact ggttcattta
     4681 gacgcatccc gccatcgaca cattcaaatt gcttaccaac aaactcaact agattatctt
     4741 cgatcattac accgtccacg atattgctct ccccaacaaa atcagcaacg aaatgattga
     4801 ttggctcatc ataaatatct acgggtgtac cactttggac gattttccct ttattcatga
     4861 caaaaatctc atcactcatt gccaacgctt cttcttggtc atgagtaaca aaaataaacg
     4921 tgattcctaa tcgttgttgc agatcccgca gttcatactg catatctgta cgtagtttca
     4981 aatcaagcgc cgataacggt tcatctaata gcaatacttt cggttcattt acaattgctc
     5041 gtgcaatcgc tacacgctgt ctttgaccac cagacatttc actaatctcc cgtttttcgt
     5101 atccaggaag ctggaccata cgcaaagcat cttttacctt cttttcgata tctgatttgg
     5161 acattttctt gatcttcaat ccaaaagcaa cgttatcaaa aacattcata tgaggaaaaa
     5221 gtgcataatc ttggaagact gtatttactt gtcgcttatt agctggcaaa tcattcaacc
     5281 gtttcccttc aaaatagatg tccccttctg tagcttcggt gaatccagct ataatacgta
     5341 ggatcgttgt tttaccacag ccggaaggac caagcagtgt ataaaatttt ccttgttcga
     5401 tctcaaaact tacttttttt aatactgttt catcatcata gcgtttcact acttgatcaa
     5461 atgaaatgat tggtttcccc acgtttttac cccctatatt acaaatatga ttgcgttgca
     5521 acaatcaata cttttgttcg ttttttgaga tgattcctta attgatgttg ttttgtcgct
     5581 tgataataga tcgactgtcc tgcttttgcc acatagaccg tctctcctaa gcttaactct
     5641 aattcccctt ctaatacaaa aataaacgtt tcagataatg aaggttcaaa cgtcttatat
     5701 tctcctgcac cttcaaaagt cagcaagaca ggctccattt ctttttcatt cgaatcagcc
     5761 acgagccatt ccaattcata cccgttttct tcatcgaaat aaaccgtatg atcttctttt
     5821 gaataaacca cttgcagatt cgctgcttct tgatgaaaaa attcttctgg agtcacacct
     5881 aagacttcaa gaatagaaaa gaatgtttcc attgatggtg aacttaaatc tcgttccaac
     5941 tgggaaatat acccttttga caaatctgtg cgttcgccta gttcttcttg tgtcaaattc
     6001 ttttggatgc gcaaatttct taacttctca ccaatttcca taatttcctc cgaatcgaag
     6061 ttttctaata gtaaacttcc tgtttactac cactcgtatt agtatacgaa atttattgaa
     6121 aaaggcaacc ctttgtaaaa ttttttttgc tgaatatttc tttatctttt ggaataaatt
     6181 ctagttgttt gattctttat ttttcaccta atttttgtct aaatttacgg aagaacaaga
     6241 ataaaagagt aggctttatg gtatgataaa agaaaatttt aaaaggagtg tctcacgaat
     6301 gaacgctttt cgatcttttc aggtaataaa ggaacctgtt ttccatacag caatcagtga
     6361 tctgccattt actgagttgg ctgaaaatga agtcttggta aaaattgcct attctgatgt
     6421 caattataaa gatgcattgg caatgagcga gtctggtcag gtgatacgaa catatccgat
     6481 gactccaggg attgatttat ccggaactgt cgtccaaagt aacaatccac gtttttcaaa
     6541 agaagatctt gtacttgcta ctggttttgg gcttggcgtc acccatccag gcggttatag
     6601 ccaatatcaa aaagtaccag gagactggtt agttcctcta ccaaaaaata tgacgctgcg
     6661 tcaagcgatg atccttggta cagcagggtt tactgctatg ttgtgtgtga acgctttaat
     6721 ccgtcaaggt atgacctccg ataaaaaagt tgtcgttact ggtgcttctg gcggtgtagg
     6781 cagtacagcg atagccctgt tacataaact aggattccag tcaatcattg ccttttccag
     6841 aaaagaagag tctgtcacat ggctaaaaag tctaggtgct ttgcaagtcg tacgacctga
     6901 tgaatttctg ccagagacaa caaaagcttt aggaaaacag caaatcgact atgtcatcga
     6961 cacagtaggt ggcgagcagc taagcagtct tcttccattg atctcttata acggtgcagt
     7021 agctttatgt ggcaatgctg ggggaatcaa actaaacgcg actgtcttgc cttttattct
     7081 gcgaaacatc cagttgattg gaattgattc tgtcaatgta ccaatagatc aacgtctttc
     7141 tttatggcaa cagatggcgg atttgcagat tgcagatgaa ctggtcgtcc aagagattac
     7201 tctggatcaa ttgcctgaaa cagcatctaa actacttgct ggaacacacc aaggaagaac
     7261 actagtaaat gtaggtgacc ataaatgaag attctaatca cagctggagg aacgactgaa
     7321 aaaatcgatc aagtacgtgc tattacaaat cattcgacag gacgattagg gcaagcatta
     7381 gctgattatt tagctgccaa tccggatacc acaatcgatt acgtgactac gaaacaagca
     7441 ctgaaaccga aaagacactc caatataacg atttatacga tcgaatctgc actggactta
     7501 tttttacaat tagaagaact aacgaaaaaa gaacactacg atgcgattat ccacagtatg
     7561 gcagtcagtg attttactcc tgcttttagt ttttcagaag aacagctggc aaaaaaccta
     7621 cccacttctt caacgcaaga agaactggac aattggtttg cggaaaatga acagacagat
     7681 accactgttt caaagatctc ttctaacacc gagcatcttg tgctcgtttt gaagaaaaca
     7741 ccaaaaatca tttcttatct acgtgaatgg cagccaaaag caaaaatcat tggtttcaaa
     7801 ctattagtag atgtaccaaa ggaatctctt ctggcagtcg caaaaaatag tctgataaat
     7861 aacaaaactg attttatttt cgcaaatgat ttgacggaaa tccatggcga gacacaccac
     7921 ggctatctct tatctaaaga tggaacagtg gaagaagctc aaagtaaatc tgagattgct
     7981 gcattgatta ctgagaaaat tagactggag gaatcaaaat gaaaaagatc ttactaggcg
     8041 ttactggaag tatttctgct tacaaaagcg ctgatcttac aagtcaattc gtcaaattag
     8101 gtgcccaggt agatgtgatt atgtctaaaa gcagcacaca ttttatcacg ccattaacct
     8161 tgcaatctct gtcgaagcgc gcagtccata cagacgtcat gcaagaatca aatccgcagg
     8221 taatcaatca tatcgaacta gcaaaacaag ctgacttgtt tctcatcgcg cctgctacag
     8281 caaacattat cggcaagcta gcaaacggca ttgcagacga tcttctttca acagtcgcaa
     8341 tggcgcttcc agtgggaaca ccaaaattga ttgcccctgc catgaacacg aatatgtatc
     8401 aaaacccgat cactcagcgt aatctggaaa cattgagatc tgtcggctat caggaaattg
     8461 aaccaagaga atctctttta gcttgtggag attttggaaa aggtgcctta gctgatattc
     8521 agacgatcat cgaacaggtc atgctgactc ttaatgaaag aaatgcttag aaaaaaggag
     8581 tacttatgaa aaatacaaaa aactttacat taactgcgat gtttttagct attttgattt
     8641 tattagctgt tacgccttta ggttttatcc ctatcggtcc aatcaatgca acaaccatgc
     8701 atattcctgt aatcattgct tcgatcgttt taggaccaag actaggtgct tttcttggcg
     8761 gtacatttgg actgatcagc atgatccgta gcacagttat ccaaacacct ttgtcttttg
     8821 tcttttctcc gtttatccca gtcatcggaa cagaacatgg aagctggaaa gccttgctga
     8881 tcgcgtttat tcctcgcatc ttgattgggg tggttcctta tttcgtttat aaaggtctgc
     8941 gcaaactaat gaaacaaaaa gcagattccg tttctttatt cttagctgga ctttccggtt
     9001 cattgaccaa tacgatttta gtgatgaata tgatctactt tttattccag caagattatg
     9061 ccaaagtcat cggaacaaat ttaaatgctg tttattcagc tattctagcg gttattttta
     9121 caagtggtat acctgaagga atcgtcgcag gtcttgcgac tgcagcagta tgcaatgtct
     9181 tgttaagata cttcaagcac ccttctctta ctaactgata gtcatcgtag ccgatctctg
     9241 atttttttct cttttggtgt tactattgaa tggaaagatt tgacgtcgaa aggatgatcc
     9301 aaatgaaaat ttttgttgta ggtgcaaacg gacaaattgg ccgacacctg ataaaagatt
     9361 tagctccaag ttcccatgaa atttttgcag gggtgagaga tgttgcttcc caaacattag
     9421 taaaaaaaga gaatgtttcg tatgtctctt ttgatttaac ttggtccgtg gaaaaaatga
     9481 ccgaagcatt caaaggcatc gatgtattga tttttgctgc cggctctcaa ggaaagaatc
     9541 ttctgcaagt agacttggat ggcgcaatca aaacagtgat cgcagctgaa aatgcgcatg
     9601 tctctcgcta tctgatggtc agtgctgttt atgcagatga accagcaaaa tggcctgagt
     9661 ccatgacaga ttattatatt actaaacatt atgcagatga atggctgaaa cgaactaact
     9721 tagattttgt cattcttcaa ccagtcacat tgactaacga tgaagaagtt acttccattc
     9781 aattaactaa gcctaatgag aaggcatcaa aaacgatcac tcgctctaca gttgctgctg
     9841 tacttgccgc tctagtggaa gaaacggata tttctcgtac gactctagta ctttctgaag
     9901 gtagcaaaga gttgaataca gcattccaag aatgggcaaa ggaggaatag atccatgcgt
     9961 gccatttcaa tcaagcatcc tggttctgta gatgtattag aacaaattga agtgcctgtt
    10021 cctaaaccaa aacaaggcga attactgatc aaagtccata cagcagctgt caatcgaaca
    10081 gatattatga aaagagaatc agtctctttg aaacctccct accctatttt gggagtcgaa
    10141 gtagctggag aggtagttga gaataaaagt gatatctcca cttttactcc tgggacaaaa
    10201 gtatacggat tagtcaatct aggtggttat gccgaatatg ctgtgatgcc tgccgatcgt
    10261 gcaatccttc tccctgatac gctagactat gtttctgctg ctggaatctc agaagttttt
    10321 ttgactgctt atcaaacact ttactggctt ggaaaattac aagaaggcga aactgtcttg
    10381 atccatgcag gagcaagtgg tgtgggaaca gcagcgatcc aattagctag acagctttct
    10441 cacgcgaaaa tcatcgttac tgcaggatca gaagaaaaac tcgccttttg taaagaactt
    10501 ggtgcagatg aattgatcaa ctataaaaaa caagactttc aagaagaggt acgaaaaatc
    10561 actgaaggtc ggggagtcga tgttattttg gatttcatcg gcgcttctta ctgggaaaag
    10621 aatttagctt ctatagccgt tgatggacgt tgggtcttga ttggtatgct aggtgggacc
    10681 atcgtgcctg agatagacct tggtgtattg attgggaaac gtgtccaact gcttggtaca
    10741 ttattgacac ctagaagtga tcagtataaa gcagagctcg cacaaacttt tatgcaaaaa
    10801 gctgggcctt ttctagaaaa tggcaagatt cggccaatca ttgaccgtac attccaattc
    10861 agcgcagtca aacaagcgca tgaatatatg gaagcaaaca aaaacatcgg aaaaatcatt
    10921 ttgaagataa ctgaataaaa tcatgctgat caaaaaagag gctgggacag aagcgtttaa
    10981 ctccaagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg aatttcagct
    11041 tatttccgaa ggaatttctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa
    11101 agtgttcttt acttttgtcc cacgctcttt ttccgaataa acggtgttca aaagcttgca
    11161 tctgcggtaa taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc
    11221 ttattacttg atgcagggca gctttttcac aacctcttca ctaaacagtg ttcaatcagc
    11281 tgaccctcgg tattagctca aaacgctgat ttcacaacct ctttttattt acgttctatt
    11341 tcattcattt tccgaagcaa atcgagctgc ttgtatgccc gcttgtcgcc caaaaataat
    11401 gatctccgca actgaatttc caccgatccg gttgtttcca tgtaagccac ctgtgacttc
    11461 accagcagca tataaccccc taattggctg gttttcttta tctaatactt cagtgttcgt
    11521 attgattttc acaccgccca tcgtgtaatg gattcctggt gcgattttga tcgcataata
    11581 attcggagac gataagtcat gttccatggc tgtggcacgg ttgaattgag catctttttt
    11641 ctccgcaaca tccgtattcc atccatcaac tgtttttgct agtgtttctt ttggtaaatc
    11701 gattttttct gctaattctt ccattgtctt tccagttgtg acatatcctt tttggtcata
    11761 aaattcaata gctgtggcac gatcacgcac gccttggtca aaaatcaaat aagctgattt
    11821 ttcaggcaat gctgtaattg ctgcagatac tttatccctt gtatccattt cattgacaaa
    11881 tcttttcccc tcttgatcga ccaagattgc tccttctccg cgaacaacct caccaatcaa
    11941 gaccccctca tcctgctgca cagttggatg aatttgtatt ttatccatat caacggtttg
    12001 tccacctaat ttttcaatca tggtgatacc atctccagtt gatccttctt gatttgtcgt
    12061 aatataatca gctaaatcgg gacgatattt ttcaataaat tctttgctgg caccaaaacc
    12121 gcctgtggta actacgattg cttttccggt gacctctttt ggttcttctc cttgaacatg
    12181 tacagtcact tcattgaccg tttccccatt tttcttaatg tctgtcacat ctgcgttgac
    12241 aaaaataggg atttcccgct catggacgtt attcaataac ccctccacta agtatccacc
    12301 aatagccgag ccatcagacg gacgatgtgt cctcttttca ctcatccctc cagtgatcgt
    12361 taagttgtcc agtttgatac cgttttgatc tagccaatcg accgcatcag cagagtgatc
    12421 gacaaagtat cgaagcattt ctttatcatt cgttcctcca ccacctttta gcgtttcttc
    12481 atagaaggca tcattcgagt ctttgattcc ttcttctttt tgaaattttg tttctgaagc
    12541 attcataccg cttgatgatt tagatgtgtt tcctccggca acaggcatct tttccaaaat
    12601 tactggattc aacccagcat cttttgcttc gatagctgct gccatccctg caccaccagc
    12661 tccaataatc acgacatcat aagattcttt catttctttt ggatctgtat atacttgttc
    12721 agaagcgcct gatgatactt cggcagattc cttcgtacta gaacttgtct gcgctgtatt
    12781 tttctgatct gcgccgcatg cgctcatagc aagaagtgca acggctgtta ataaaccagc
    12841 aactaatttt ttcatttgta tccccctttt ctccttatac tatattaagt gatttttttc
    12901 acaatatcaa ctctgttttt ctttaagtca tatagctttg gtaaacacac gcttcttagt
    12961 ataattaagg atatacatga aggagttatt ttatgaatca tttgaaaacc ttttttacga
    13021 acagtacaaa aactgcaggt agaatcacaa acggaatcta tattttatta cgtatcatcg
    13081 tcgttattat gctagtggat caggtctgga accaacactg gggaaataca atgcttttat
    13141 tactgacatt aattttattt gaaatccctt attttctgga aaagatatta aagatacaga
    13201 ttccaaatat gctagaaatc attttgttat cttttatttt tagttcaaca gttttaggag
    13261 agttaagtga tttttatggc tatttttcaa tgtgggatac ttttctacat gcattcagcg
    13321 gatttttagc tgggggtgtc ggcttttccc ttgtttattt gctcaataaa aatacaaaaa
    13381 ccatgaactt gactccttta ttactagcag ttgtttcctt ctgtttttcg atgacagttg
    13441 gtgttatgtg ggagtttgtg gaattttcag cagataaatt actgcaattc gatatgcaaa
    13501 aagatacgat catccagcaa atcaatagtg tggagttaag taaagacgga aacactgtct
    13561 atcatgttcc agatatcact cggacaatca ttgaaagtca aaatgcagat ggtaaaacag
    13621 ataaaacaat catccaaaat ggttacttag acgtgggaat catggatact atgaaggatt
    13681 tatttgttaa ttttattgga gcagttgttt tcagtgtatt tggttacctc tatagtcgaa
    13741 acgattacca aaaatttacg ttcatcaaga attttattcc taaaaaaatc aaacgataaa
    13801 acatgctaaa aaacaagaga aggggcagtg acaatacttt tgtcactgcc ccttcttttt
    13861 tgaatgtatg gcatgccatt agttatccga tagtattaac tcaaaaaagt aagatatgag
    13921 gaatgactat ttcgtttttt atcccaatac tgaggactta tttcaccatt tcgttattta
    13981 tctaatttac ttaaaacctg cataactttt tcataatctg gattaggtgt gccgtctaga
    14041 ttttcagcaa taaaagtaaa acccattttt ttataaagag caatcgcctg atggctccat
    14101 gtctgtgtgt gaagatagat tggtccgtcc gtatggaggg taggaaattc ttgcaagact
    14161 ctggcagtca atgcttttgc gagccctttt ccttggtaat ctggtttcac tgctaaccaa
    14221 tgaaaaaccg gatacacttg ctctcttgat ttttttcgcc aagcggtaca ggttgctact
    14281 ttttctcctt tttttgtctc tacaaaaaac atccttcttt caagttcttc aggataagga
    14341 gcaaagtttt gttgaaaata ctcaagtgct tcatctgtcg tttgaaattc cccaacggaa
    14401 gtctcaatct ccgcccaagc agattcatct tctttttgat aaaatcgaaa ttcaaaacct
    14461 tccgctaaag gcgtgattgg aacattttgt tgaagtggtc ttgtcatcca gatttctgcg
    14521 taaggtaacg ttttatctaa catttttcta tccctcctcg tctccattat gccaaataac
    14581 atatgactca gctacttatt tgactataac agatagagat ataaatcaga accagtcatc
    14641 tgcgtcaaat tcatttcgat tagcgtatac ccatgttcct gccatacctc gcatgaaatc
    14701 tcctaaagca gaagaaggaa tacctccacc aggtgcgggt gttcccggac taggaagtgg
    14761 tacttgcggc tgcagatgct catcggtgga atcaatagtt gacaagcgtt ttttcttcgt
    14821 ttccttctgg tcatctgatt ccttctgatc ttccgactgt ttagcaacaa attctttttc
    14881 tgattgttgt tttattttag gctctaaaat ttcattttcc aatccagaaa cttgtggggc
    14941 caaatgagct tcttgcctta tcgttttatt agattctgag atttttctag atattcttgc
    15001 taactctagg cttcttgttt gttttacttt ccctgttgga gaaactgcat gagccatttg
    15061 ttgttttttt tctgacaagc tttcttcttg tcttttttgt tctttttgat ctatctgttt
    15121 gtttaatggt aaaacatgta atagtgactg agcatggagt tcttggagtg aatacgggtc
    15181 atgataacta tacataatcg taaaatcacc tgtttgtttg gtcaaataac catcctccaa
    15241 tggtacttca ttgattgtaa ccgatacatc ctttaacggt acgggtttac ctaaggaatt
    15301 tgttgctgat tggatcaact cttctttgtt tattttttcg tttggatgaa caataagatc
    15361 atgaccttct aatgaacgat tgtctggtat ataaacataa gtaaaggtaa tttcccctcc
    15421 tgattgcttc ctattattca tcagttctga gtgaagtact tcaaggatct ccgaccagtg
    15481 actttttctg tctaatccgt ttgcttgcat ccaatcagaa atagaagtga ctttcccatt
    15541 ttgttcataa gtcgataaat ctatatttgg cactcgatca gaaaattcag caggtaggcg
    15601 caaatcagga atacgattta cactactcat atgtgtttct gcttgaagtt ctttatttgt
    15661 ttgactatct ataaaatgag tgatgacagt cgatgaagca gcagatgctt ctacataaac
    15721 tacagctgtt gtttctgcca catttgatgt tttcgagtct atataagtta atacaacggg
    15781 atattctccc ggagttgtag tatctactgt cccgttcatt ttgatttctt ctttatttcc
    15841 tgaagttcca tcgctttttg ttacatattc aacaagatct ttaggggcgt aactctctcc
    15901 tgttcttaag tgaagatctt ttcctttgat agctgtttga tcagcacgaa catggatcac
    15961 agcagatgca gtcattgttt ttaaagcaat tggatcaaaa taggacaagg aaatagtaga
    16021 tgttcctgta ttagtcgtat ccacgatatt cccattgttt gaaattactt tggacatatc
    16081 tccttgtgtt ccatctgcgt tcttcagcct ttctaccaga tcagatatcg aaacttttgt
    16141 ccctgccaga acatcgattt tctttctttc aaactgtgaa taatcttttt catatacata
    16201 agaaacttgt gcgcttccac cgtcctttat atctgtcaaa gcatgattca aaatatcagt
    16261 gatctgtttt aactgatcac cttttgtagg atcagtcata tcggaaaaga tagaaagcgg
    16321 cacactgctt ccggtttccg atgtccataa tgttttgtta ggaattaacc agtagtggtt
    16381 tatcgttgga ggataactga ctttattata ggaagtcaat gcaggaagta gctgttttcc
    16441 atttgcatca gtgaaagtat gagtaatagt gataggggaa tcagtggtct cagaaacaga
    16501 ggtacgtacg accttgatgg tttgtgtcgt tgcctgaacg ggtacatgca tatagacacc
    16561 tacagataaa ctgatcagca tcgtgcgcat cataaactta acgtagtgtt tttcagaaca
    16621 cgaccgttcc ttatctaaac gacaagcgtg tttatttagt tgttttgaac gtctttttga
    16681 acacatttat tcttagtcct ccctactctt gtctaaacat gaagtagtaa ttttcataag
    16741 atgatttcat tatataaata aaatcaaaag aagatggact attaggagag tatttttttt
    16801 gattttttat tgtttcaaga aaatcggaat aataacttat gtacttttta attttgttaa
    16861 gtaatatatc tagttatatc tagttatatc tagttacctt ttctttgaaa aaaacataaa
    16921 tcagctaata aatatgtgag acaaagctgc catccataaa tactagctga ttttttcagt
    16981 taaatgtcta tttttttctc tgatgatggt atttccaaaa tattcccagt catctggagt
    17041 cggtggattt ttccaaatta tctgttcttt attaaatttt tcaagtaaac aatcagataa
    17101 ataaatattc gtacgagaag tgattcgact ctcaatcaca atattgtaat ttttaaatcc
    17161 tttcacttga gaaataatgt aatcggtata agcagatcct tgcgaaaaat caatacagac
    17221 atgaatcggt gcttcaaaat atgataccgg acaaataccg accaataaaa agagataatc
    17281 ataaaataag cgaatcatga aacgttcatc ttttcccaag aaatagcgtt tattctcttg
    17341 tatgaccttt tggattgctg tactgaatat tggatacgtc tcgaagaaaa actgtagctg
    17401 ctttttagac gtaaagctag tcatctcaaa gtcgaatatc caattttttc gattgacatg
    17461 catcataccc atacgaagtt cttttcgtat atatagtctt tcttcttcag agatttcaat
    17521 cgaaaatggt aattgatcaa tcaattccac tgacattttt tgactaatca tgtggatatc
    17581 atgaaaatga gcttccgcaa gtccaaatgc gccatctaat tcaaattctt cttccgtgat
    17641 caaaaacaac atgatataag aaatctcatc aatgatctgc tctttaggga tatgaagaaa
    17701 agaaatatgt gataaatcct tgatgatctt cattccttct gagctcttaa agtattcatc
    17761 tgaaaagaaa cgttttccga ttggttgatt cccataggcc ctcaacaaac taatagtggt
    17821 tagcaaattg aatttgattc gttttgttgc agacagtttt aaattgaaca gatattcaaa
    17881 atattgcacg atactctttg cttcattcat gacgctttct ctaaatggca tagaaaaacc
    17941 attaaagacc ccgaaatata ccccaaataa tacgttccgt atgtttattt cttccccaac
    18001 taatatgttt ttctttaact ttatttgatg ctgttcaaga aaagagacta attctcttct
    18061 ttttacatag gctcttgata tgcttaaaaa atgattctct gcaaattttt cgatcgttac
    18121 ttttgaatcc acgatctccg ttaacagtcg agcaattttt gatcgttgaa agtagattct
    18181 tcttaaattt ttgatatccg tcaaattcag acgttcgaca tggatctctg cttcttctga
    18241 aatttggatc tctggctttg ttttgaattc agtaaattct ttatttaatt ctatgatata
    18301 atttcttgtt ttaaatttag acaaacctaa tgtttcatat aacttaggca gcactacata
    18361 attctttttc tcaaattcta aatatctagc tagcagcagt ttttctctat cttcctcttc
    18421 tataaggtaa tccaacttca tatcctacac ctcaaaaaat gcctcatacc aattacttga
    18481 tatgcagatg aaagttcatc ttttcaccta tactgatttt atgatggatt atgcttgttt
    18541 tttcatcagt tattctcatt ttttatcttt tttttgagaa tattgcaaaa aatggaaaaa
    18601 tttatgagga aatattctat attttttgtt ttattgtgtt aaaatgtaca agatttgcgc
    18661 catttgcgta aacaaccaca aaaaagagca ggacggccac cgtcctgctc ttttacttat
    18721 tttaagttcc attacttatt aattggctaa acttctgctt gtttctgata ttgcttgttt
    18781 ttctccaatg atccgcactt ctgtttctaa agacacatca aatttttctt tgattacttc
    18841 ttggatgtgc gcaatcaatt caatataatc tgtcgctgtc gcatgatcga tattgacgat
    18901 gaatccagca tgtttttcag agacttgtgc accgccccat ttcaggcctt gcaatcctgc
    18961 ttgttggatc agctgacctg tatagtgtcc tactgggcgc ttaaagacac ttccgcatga
    19021 agggtattct aatggctgtt ttgattgacg caattcggtc aactcatcca ttcttgcttt
    19081 gattgcttca taatcccctg tttgcaaact gaatcgagct tccaatacga tagcacgtaa
    19141 ttcttggact ttactgtgac gataagaaaa taccatttcg tcttttgtca aagttttcaa
    19201 tgttccatct tcaagcaaaa gatccacttc agcaaaaaca tctttgatct ctccgccata
    19261 cgcacctgca ttcatataaa ccgctccacc gacacttccc gggatgccgc aagcaaattc
    19321 aaatccagtc aatgattctg ccaaagctgc ataagtcgta tcaatcagtg aggcacctgc
    19381 ttccgcaatg accatcgttc ctttgacatg aatctgtttc atttcagtca gcatgatgac
    19441 tacaccgcga atcccgccat cttgaacgat cagattgctt gcatttccta ataccatcca
    19501 aggtatatca tggattcggc aataatcgat gatttgcttt acttcttctt gttttttcgg
    19561 gaatgcaagg acatcagctg gtccacctgt tttcgtaaac gtatagttca tcaatggttc
    19621 atcaaacaat atagtaatcg tggctaagct ttcaactatt tcttctttat tcactgagga
    19681 ttttcctttc ttttctcaaa cgtcctcatt attctatcac gcttaataca atcttgccag
    19741 tcttgttcaa agttgatgac ggtagtgatc tttaagcact tcctcacata attcatgact
    19801 tcttacaact tctttttgtt taagcaattc tgtttccgat ttacctaaac ggatctgaga
    19861 aaagaaggcc tctaccattg gctcaaaccc gcgtttgact agcgtgtttg tccagtcgcc
    19921 aaattctttc actttttttc catcagcatc ttgaatgaca agctgggtca gatcgtttac
    19981 ttcataagta ccatttttac ttgttacttg atatttttct atattagcgc cacttgctaa
    20041 atccatcgtt aaaatagccg tttgattttg tgtttctaat tgtaaaatgg catattccaa
    20101 aaattccttt gtttctctta tctggtattt tgttctcaaa atgggttcat ctaataaata
    20161 cacagctgta tcaataagat gtaaaaacaa atcataaagg acgaactctg tcgtttcttt
    20221 tgccgctatt cgattttttt gcaggtggat caaccgctta tctgggatac gtttcaattc
    20281 ttctaccata ggagcaaagc gccggttgaa accgatcatc aaaattattt ggttcttatc
    20341 agctaattct tgcaattcac gaacttcatt tagatggaca ctcagtggtt tgtcaatata
    20401 aacatggata ccattttcta gacatttttt ggctaattca taatgtactt ttgttgctgc
    20461 gtggatcata catgcttcga ttttttcttc taaaagctca tcgatcgttt catatagatg
    20521 ttggaacccg tactcttttt gaattctttc ttttgtttct ttatttcttg ttgcaaaata
    20581 aaaatcaagt gttccttgtt ttttcgcata agttggtaaa tatgcttttt ctgcgatatt
    20641 ccctacaccg attacaccaa tcttcatcta ctttcctcct ggatcattta gttactttca
    20701 tcttacacca ttttttacta aaatgaggta caatagtatc gcaaaggtaa ggaaattaat
    20761 tcgaaggagt gtgcttttat tgaaatttat ctcttggaac gtaaacgggc ttcgtgccat
    20821 cgtaaataaa aattttttag aagtttttca tgagttagac gccgattttt tctgtctgca
    20881 ggaaacgaaa ctccaagcag gacaaattga tttagatctt ccaggttatt atcaatattg
    20941 gaattacgcg gaacgaaaag gctattccgg cacagcgatt tttgctaaaa aaccagcatt
    21001 gaatgccact tacggaatgg gtatcgatat ccatgataca gaaggccgtc tgattacttt
    21061 ggaatattcc gatttctttc ttgtaacctg ttacacgcca aattctcaaa gcgaattaaa
    21121 acgtttagat taccgattag aatgggaaga agccttttat aattatttgg aaaatctaaa
    21181 aaaacaaaaa ccagtcatcg tgtgtgggga tctaaatgtc gctcatcaaa agattgattt
    21241 gaaaaactgg aaaacgaatc aaaaaaatgc tggattcact ccagaagaaa gagcggctct
    21301 ttctcgactg ttggataacg ggtttatcga tacattccgt tatttttatc caacccagga
    21361 aggtgtctac tcttggtgga actaccgatt caattcaagg aaaaacaatg ctggttggcg
    21421 gatcgattac tttttgacga gcaaagattt agagccagga ctagctgatg caaagatcca
    21481 tacggacatt atgggcagtg atcattgtcc agtagaatta gatttgaaat aaagaggttg
    21541 tgaaaaagtc gtttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt
    21601 ttgcataaat ttggcataat accgagaaac tggcttttga acactgttta tcgagagatt
    21661 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
    21721 ttttcgtttg gttgggctta ttaccgcaga tacaagcttt tgaacaccgt ttattaagag
    21781 gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    21841 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa
    21901 gaggttgtga aaaagctgca ctgcatcaag taataagaac agccaaacaa aaatagttcc
    21961 tcatattttt cgtttggttg gacttattac cgcagatgca agcttttgaa caccgtttat
    22021 tgagaagttg tgaaatcagc gtcctgacct gagtattaga gaaacaaatg gcaaaatagt
    22081 tcctcatatt ttgctatttt ttgagctaat accgagggtc agctgattga acaccgttta
    22141 gtggagaagt tgtgaaaaag tcgcctactt tcgagtcgta agaaattttt ttgaaaatgg
    22201 ttcctcacaa ctttgcataa atttgactta ataccgagaa gtcatgatcc ccatctaact
    22261 ccaaatcaca aggaatgagt tcaaaaacta gcttttctca acaaatatta cataaaaaat
    22321 tctatgtttt ttgttaaatt tatttgcttt tttcaaaagt atgttacagt aatttcccaa
    22381 ggaggtttta cagaaatgaa ttttgttgcc atggattttg aaactgccaa tcatcagcct
    22441 tatagtgctt gttctcttgc gctagtcatg gtaaaaaata gccagatcgt tgatgaattc
    22501 tatacattga tacagcctga gactcctttt ttctggcgaa atgtccaaat tcacggaatc
    22561 catcaagaag acgtacgcaa tgcgccgaaa ttcccagaag tctggcaaac tatccaacca
    22621 tattttcaaa aaaatcgttt agtcgttgca cataatgctg cttttgatac aaaggtctta
    22681 gcgggatgct tagattatta tcatcttgaa cagcctaatt atctttcttt atgtacagtg
    22741 aaaagtagcc gtcgcctgtt tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa
    22801 aatttgaaca tccaattaga tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg
    22861 gaaatcctgc tttatcaaga aaaaaaattc gggacagatc cattgaaaaa acttgtgact
    22921 ataaagtaaa agaatactta tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc
    22981 gagtcacaag gagcaatttc aaaaaatagc ttctcatgtt tattaaatat tgtgactggt
    23041 gaccgaggag ctggcaggag aacaccgttt attggagagg ttgtgaaaaa gctgttctgc
    23101 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttggact
    23161 tattaccgca gatgcaagct tttggacacc gtttaccaag aggttgtgaa atcagcgttt
    23221 tgacctgagt attagagaaa caaacggcaa aatagttcct catattttgc cgttttttga
    23281 gctaataccg aaggtcagct gattgaacac cgtttattca aaaaaaatgg actgcgacac
    23341 gacttttgtc acagtccctt cgtgtgtttt tctgccttct caaaaggatg acagtttctc
    23401 actcttttcc tatttcttct attcaatcaa ataactcatt ttatatacat ctaaaaattc
    23461 acctagatct gttcttgccc ctctaggcat gaccgcttcg atttggaagc ccatttttcg
    23521 gtaaaggtgt acggctcttt cattacgaac ttgaacgtca agctccaatc gaaaaagtac
    23581 acccatttcc ttcgcccaac tgatgatttc ttctagcata agagtgccta gtcccattcc
    23641 ccaatattcc tgcagaatac tgataccgac ttctcccacg tgagacaaac gaaattgact
    23701 gtctgccttg acagatgctg ttccaataat cgtcccttca tagattgcaa gcagcaataa
    23761 attattattg ctttcgtata tataatccaa ctcttcttct aaggtttctg gtggtaacag
    23821 gagttctgct tcatccaaaa ctaaaaattc tgtctcctta ttaacttgtc ccatcatcaa
    23881 aagaatgtct tttgcatctc ctggcactgc ttcccgcaag atgatcccaa cttcttcact
    23941 catgattttc agcccatttt tctagaattc gctgtttgaa ggtttcactt tgctccccgt
    24001 atgagattag tttcacatac cgcttttcta aatctgtatc acttttttct aaaatcagtt
    24061 ccaaataatc ctccggcagt gcttcttcta atagatttcc ccattcaaca acggaaagcc
    24121 catcaccttc aaaatattca tctaacccta aatctgctcc gctagctgct acccgataaa
    24181 tatccatatg atacaacgga atccgacctt gactatattc tctaataatc gtgtatgtcg
    24241 gacttttgat catttgcgtt atccctaatc cttgggcaat tcctttcgtc aacgttgttt
    24301 tacccgcacc tagatcacca gtcaatatca gattgtcacc tggcattgcg gaatgaccaa
    24361 ttgcctctgc gaattgttct gtcattgtta atcctgatag ttcgatcatt tttcatcttc
    24421 tcccgctctt ctttcttttt gccagtatat cgcgtaatga agttttttta aagaaaaaat
    24481 gaactagtca agaacagcag ctgctgtttt tggctagttc gcgtgtattt gttaatcaat
    24541 caatgtttgt gctgctgtga taatcgataa tttatataca tcttcttcat ttgctccacg
    24601 agacaaatca gaaaccggtt tgttcaagcc ttgtagaatt ggtccgattg cttcaaagtt
    24661 accgaaacgc tgagcaattt tatagccgat atttcctgac tgcaattctg gaaatacaaa
    24721 gactgttgct tgaccagcta cttctgaatc tggcgctttc agttgtgcaa ctgaaggaac
    24781 ataagaagca tcaaattgca attcgccgtc gatcatgtat tgtggcgcta gttttttagc
    24841 gattttagtt gcttcaacta ctttgtctac ttctggcgat ttcgctgagc cttttgtcga
    24901 gaagctcatc attgctactt ttggttcgat atcgaacaat tctgctgttt ttgcagaatc
    24961 tacggcgatt tcagccaatt cttgcgcagt tggattcacg ttgatcgcac aatccgaaaa
    25021 gacatatttt tcttgatctc ttccacgtac catgatcatc gctccgcttg tccgactgat
    25081 gccaggcttt gttttgatga tctgaagtgc tgggcgaaca gtgtcgccag tcgaatggat
    25141 cgctccagag accattccgt ctgcaatccc catatacgta agcatcgtac caaagtaatt
    25201 cacatctttt aaaatctttt ctgcttgttc tttggatgcc tttccatttc tacgttcaac
    25261 aaaagtatcc accatttcat cccacttatc gtaattatca gggctgatga tcgtaaagct
    25321 agatgttttg attcctctgg catgcgctgc atttcttatt tcttcttcgt tccctactaa
    25381 aatgggttcc acaagttctt ctgctttcaa ccgggcagct gcgcctaaaa tacgagcatc
    25441 agtagcttcc gggaaaacga ttttgatccc acggcgaaca attttaaatc ttaaactatc
    25501 aaacaattcc acaagatgac ctccagacgt ttttatatgt attatcatac actattccag
    25561 aaaaaaagaa cagtttattt tatctgtatc agtcaaaaat gctggaaaaa ttaagttttt
    25621 cacatacaaa gcgaactgca ctataacagg aaagcgctgt tttattacac tgtttcaggc
    25681 agttgccaat caatcggttc ttctcctaac gcaaggagcg catcattcgt tttcgagaag
    25741 ggtttggaac caaaaaagcc acgacttgca gataacggac tgggatgtgg agatgttatg
    25801 atgatatggc gactcttatc gatcattttg attttttctt gtgccggttt tccccacaag
    25861 ataaacacga ttggtttttc ccgttcattt aatttttcaa tgatcgtgtc tgttaatctt
    25921 tcccaacctt tcccgcgatg agaatacgct tgcccttccc gaacggtaag aacggtgttg
    25981 agtaaaagga ctccctgttt agcccatgac actagatttc catgttgaac aggtgcgatt
    26041 ccaagatcat cataaagctc tttatagata tttcttaacg atggcgggat ctttacgcca
    26101 ggttgcacag aaaaagataa gccatgagct tgattttctc catgataagg atcttgtcct
    26161 aaaatcacca ctttaacttc ttcataagga gtcaattcca atgcttcata gatatgatac
    26221 atatctggat ggatcttttg ggacgcatat tcttttttta aaaattctct tagatgcaga
    26281 taataatctt ttgaaaattc tgaggacaaa acttcttgcc aactgttatg aatgatttct
    26341 ttcaaaaact ccacctccta ataaaaagaa taactcacat tttcttcaat cacaagggac
    26401 aatcttcacc ctcagcactt gattaatatg gtaaactagg aacaagaagc agaaaggaac
    26461 gataatcatt atgatcaaat taattgcatc agatatggac ggcacattat tagatgccca
    26521 tatgagtgtt tctcctgaaa atgccgaggc gatacgtttt gcaaatgatg ccggtgttga
    26581 gttcatggtc gctactggtc gaaactatca agaagctcgc gcagctttag atgaggtagg
    26641 aatcgattgt gcgatgatca ctttgaacgg ggcacaggta tttgataaag aaggaaattc
    26701 attgtttacc gtgtcgattc caaataatca agcaatctct gttttagata ttctagatgc
    26761 taacggtatt tactatgaag tagcaaccaa cgatggtctt tattctgaaa atcaagcgaa
    26821 acgaattgaa agttttgctt ccatggtggc tacccattta cctcacctta cgtataaaat
    26881 ggcgattgcc atggcctcag ctaagctgga gttattgcac atcacatacg tggacagtat
    26941 tcgtgctatt ttggaagaaa agaatctgga agttttaaaa attatttgtt ttcatacaga
    27001 agggccagct gtattaggtc cagttggaaa gcagatttct cttttaggcg atctagccgt
    27061 tacttcctct ggtcaaaaca atatcgaagt gaaccataaa aacgctcaaa aaggaattgc
    27121 tgttgcccat gtcgctcacg agcgtgggat ttctttagaa gaagtcatga cgattggcga
    27181 taacttcaat gatgtcagca tgctgcaagt agcaggagtc agtttcgcaa tgggaaatgc
    27241 agaaatcgaa gtaaaagatt atgccaaata tacaacggac acaaaccttg aatctggcgt
    27301 aggaaaagcc atattgcgcg cgattgcaga agacttgtag ttttatctag aaaagaggga
    27361 tatagatgtc agaatttttc attcaggaaa agcagcttgg acgtgcaact cggacaattg
    27421 tcaaaaacga aaaaggacat tccctgtttt tgatggttgg tcgctgggga acaaagggcg
    27481 atgcattgtc tctttatgcc atgaatggcg atctcgttgc tcacatcaag cagataagcc
    27541 tgacattcgg tacgcgtttt gagctgtata aagagttcga aaaggtggga accatgcaga
    27601 aaatattcaa ttggcccggt gacttttatt atattcgtag acttcactgg acagtccaag
    27661 gtgatatcta taatcatcaa tatcaaatcc atcatttcaa tcagcaaatc atgaaaatgg
    27721 ataaagcaac acttttttca ggagattact atgtattgga tgtctcaaat gatgaagatg
    27781 ctccagtttg tatttgtatt gctgcggttt tagattactg gctctaccgt aaagacaaaa
    27841 gtcgcgataa gcctgtttct taccgactta acttaagtta aaaagatgta cctgaaatgc
    27901 ggaaatacgc atttcaggta catcttttta gatattcttt tgcatatgat cataaagatg
    27961 accactgatc atccgctgac cgacaacttt gaagtcatgg cgttcgtata attttttagc
    28021 ctttggattt ttttcatcca cacttagacc gatgacggag cgattagctt tcttcgccaa
    28081 tttaggtaat gcttctaata atcgcgaacc gatgccttgt cctctaaaat cttctgatac
    28141 actgatagag tccaaatacc attcgttggg aaacgtttct ttatcaatga acaaacgtgt
    28201 agacgcatct agctgatatt tctctaagat aggtaccagt ggctgatcga tgattgattc
    28261 ttcttcatct gtataaccgt aagcaactcc tgcaacttta ccgtctattt cttctacgat
    28321 acctcgtgaa taactgtaac gataatttgg atcagtgatt gcttcttcta aaatctctaa
    28381 agtcttttgt tcccctgtct gcttcaaaaa tggcagttcc atatctttta gaatcactaa
    28441 gatcaatggg gcgatttctg gagcatcttc ttttctcgca aaacgaatca tagtttcctc
    28501 tccttttctc cattctaaca tgcagaaaaa gaaatcgcaa aatcgttatt ttaaaaaatc
    28561 ttcaatcttt tcagtaatga aataaccatg agtaacgatt cttgccgctt cagttcggca
    28621 ataagggcag acgactggca tttctttatc attatgttcg actacattat tgacttcttc
    28681 gtatgcaaat ttttttccgc aattagggca tttcattttt tccatgttat ctcaacttcc
    28741 tttcatcttt actctcttag cttactctta tattgtaaaa aagagaagca atatccttct
    28801 tctatgcaga tatccatacc tgacggtcct gttctccatc tattaatcgg actttataat
    28861 aaggacgttc atctttgact agaagtgtcc actctttcac ttttgctttt ttcccgtagt
    28921 agctgactgc ttccttcatc gctgcttgcg gttgtttgaa ctcttccgtt ttttctatat
    28981 caaaaagatg ttctgcggcc ttttccgtgt catttcgtac ggttgttttc ccagtggatg
    29041 gattgactgc tatgacttgt ccttcacaaa aaaactggta ttcatatgtt gcattcgtag
    29101 gattctccaa atctttttca actgcaaaat gaatagcaga tatgttatgt tcttttgttt
    29161 cgttttttac gattgctgct gcttttttca atgagatccg atattcctgg tcactaatat
    29221 catccatgtg aaaagcggca ttgagcagtc ccgtcatacc ggtaatccct attacgatga
    29281 gggcagcaac accaaacatc atttttttca ttcttcattc tcctgtttct tttttttaaa
    29341 gaatatcaag aaatgaacga atgaaaaatc gaatgaaacg aaaaaaaagt gacaattttg
    29401 ttatctttat tcgtgcagtt ctaatggcaa tccgtctgga tcaaagaaaa acgtcatttt
    29461 ttttcctgta aaggtatctt ctctaatcgg ttctgattcg atccctaagg actgcaattc
    29521 tgcaattgtt tcttcaatat cgtctacttt gaaagcaaga tgacgcaagc cacaagcttc
    29581 tggatagttt ggtcgttgtg gcgcattagc aattccaaaa atctccaatt cagcacttcc
    29641 tagtttcaga tctaatttat agtcatctcg ttctttgcgg taattttctc tgattacttc
    29701 aaatcctaat ttattaacat aaaaatcacg tgatttctga taatctgaaa caatgatggc
    29761 tatatgatgg atcgttgtaa ggttcatatg agcactccta ttcttcgttt aagctcggat
    29821 ctatattaat ctccagttcc attgtaaatg tgtcagattg aaaaaaatag ctgtttttcc
    29881 tttttataag aaaaaaggca aaaaaaacag aaagtaggtc ctccaagaca ctcttcctgt
    29941 tcccaatata ataaatgaaa gtaagaaatt atattttcaa gaaaacaaac agaaaattat
    30001 cataacacaa attatttata attgaaagac tagttttttt tcaagaaaag catacaatag
    30061 attgtgttag taaatacaga acacactaca tttagtgtct taaggagtcc tcatacatga
    30121 agtcatattt tcagttttta gctcatcagc taaaaggctg gccgcaacaa aattattatc
    30181 ttttcttttt cagtcttggc tgccaggtca tgactcttgt caatagtccc atcacttggc
    30241 tgtccgttat tacatttatc ggtaccacgc ttggtgtatt gtgtatttta tccatcaatg
    30301 cggcaaaatc agtcaacggt attttaggta ttttgtctgc gatttgtttt attatcgttg
    30361 ggttttcagc aaagaactat ttaagtatcg gtgaacagat tgcttatatg atcacattgg
    30421 atatccccgt attgttaagt aaagaatgga atcataatat ggcttcaaaa atccgaaaat
    30481 tcactggaaa aagctggttt atcgcaatca tcagtacttt ccttgtctat cttgtttcag
    30541 gattcctgat tggtcgttat acagatgatc ctcggccgtg ggtagatgcc attgcttttt
    30601 ctattagctt gactgctggt atcatcagtt tcttgcgtta caacaaccaa tattactggt
    30661 ggttagcttc agggcttgct caaatggtcc tttggtatat ttcatttacg catggatctg
    30721 caagtttggc gatgttcgtg aacagctctg tttacttgct gaatgatgtc cttgcattta
    30781 ccgtttcacc ttggtataac caaaaagaac gtgaacgtat gcaaaaaatg gaagaagaat
    30841 actacaacgc acaacaacac gcataaaaaa actgagcgtg ggacaaaagt aaagaacact
    30901 tttgtctcac gctctaaaca cgtataaacg gcggaatcag aagcaactcc ttcggaaata
    30961 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
    31021 ttaaacgctt ctgtcccagc ctctattttt tatagtaatt ccttcagata ctttcttgcc
    31081 tcatcattga aacgcagtga ttcttctaac ttctgataat attccaacgc ttgtttctga
    31141 gcttcagtaa aacgataatg ttttcctttc catttgtcga ttgtctcttg cacatttcca
    31201 gattggatat ctttcactac tagttgccat ttttcttttg agactgtacg gtctttctta
    31261 tcagcagatt gtatagcttc tcgttcttta cgcaatactt ttaaaaaacc atttctgact
    31321 atccgaggaa tgctgtcgaa gaacccttct gtaaaaccat agcgacaata ttgcttccaa
    31381 ataaatagat agatttttgg ttggatgaat cgttggtttt tcgcaaaata ttccatctga
    31441 gcagtgactt ctggtgttcg ttgacgtttt gtctttctca acacggaaat aaatgtttca
    31501 aaataagcat taggatgtac tggcacttct tcatatacga tggattgcca atctttacgt
    31561 aaaaatgcgc gatagttgat caagaattct tctacatcct ttactaattc ttctgcaaag
    31621 tcgtcaaata gttctttttt tatctgtcgc ttttttgact gcccattgat gtgttcactt
    31681 attttcttga tttcattttc caatgcttga taatccgcta tgtaaatcgg catttctact
    31741 tggcgaaatt ctgcgagccg ctgagctaat tttatatccg gtaaatatgg ctgtttcatc
    31801 cgacgatttt ggtacaagac aaaacagtat ttttgccata gtccttcagg aaaatcgata
    31861 ttcttctgct taagccataa taattcgtct aaggccagat caacttttgt cattccctga
    31921 tggatcgaag ctgccactgt cggagagaca tgcaggtgat cggcaaagtg gttgatgcct
    31981 agttttagga tctttccggt ttttggcgat ttaacgatgt attgcacttt taattctcta
    32041 ccacactcac aatacaattt ttgctggctt cgttttgttc gaaaaaacgg attgatcttg
    32101 aagtcagaaa acacccaatc tgttgcagct aaataatgtt cttccataaa acgagagctg
    32161 attagatatc ggcggtgctt atcgactagt tcccgctgtt ttttcgttaa ttttgcatag
    32221 atctcccaac gttgactgct tgtgtattgt ttagaaattt tgatgggata gcttttgctg
    32281 atttcttttg gaaaccattc ttcaaagacg gattgccatt cttccacggc tgtttctttc
    32341 ctttctaatt acaaaaagct ggctactaac ataacgttag caaccagcca gtgtttcttg
    32401 ctaaattagt gagttgttcc ggcatttccg gaaatatctt ctttattttc tactgctgct
    32461 tcaactgcat aagtcaaaga agaagcaatg tcttctaaag acatagaagg cgtacccact
    32521 ggacgatcga tcacttgatc gggtgagaat ggcacgtgga tgaacccacc tcgaattgcc
    32581 gggaattgtg tttcgatcaa atgaagcaaa cgatacatga tatcattgca gacaaatgtt
    32641 cctgctgtat aagagatgta cgctggtaga ccatgatctt gtacattttt tgtcattgct
    32701 ttgattggca atgaggtaaa gtaagcggct ggaccgtttt cttctaaagc cgtatcgaat
    32761 ggctgatttc cttcattatc agggattctt gcttctgcta gattgattgc gacacgttcg
    32821 aacgagactg ctgaccgccc acctgcttgt ccgacacaga tcacgacatc tggttggtgc
    32881 tgttggatgg cttctttgac tacttgactg cttttttcga atacggtcgg tacttccact
    32941 ttgatgattt cagcaccgct gatttcatct ggcatttttt ttactgcttc ataggctgga
    33001 ttcactttgt ccccaccaaa tggatcaaaa cctgttacta gtactttcat ctgttgttcc
    33061 tccgtccgat tgatttaaca tatggttatc ttttatcata ccattttcct tgaacagcgt
    33121 aaataaagga aacaatagtt taaataacat aacagatcaa acttaatata ttcttataaa
    33181 aaaatagtgt tccactacaa tgttagaaga ggttgcgaca attattctgt cacaacccct
    33241 ctcttctatt atttaaatag aatcatataa gcaatttgga aaactagcat aaataacgca
    33301 ataaacaact ggtttttgat cactccgtat ttgtctttca tctccagcat cgctacaggc
    33361 acgatattga agttagcagc catcggtgtc agcagggtcc cacaaaaacc gcaagtcaat
    33421 gcaaccattc ctattaacgc tggattcgct ccatgactaa atacgaatgg tgcaccgatc
    33481 ccaaccgtca tcacggtgat ggccgcaaat gcattcccca tgattaccgt aaatatcacc
    33541 atgccaagag cgtaaatgat gatgccgatg ttcacatttc cttctggaac gatcgtacct
    33601 actgcattgg aaatcactgt cccaactcca gcgcttgtaa ataccgcacc tagagaagct
    33661 aaaagcattg gcaacatgct taacggccct actgtgccca gcatatctgc tgcatcatct
    33721 aaaaatgtac ttggtcgatt atctcgagaa aagaacatca agattagcat ggctgcaaaa
    33781 acaccgacgc ccacgccgac taaagcacct aaattggtaa atagagcaaa caagatcgca
    33841 aagacaccga tactcaaagc tggaataaaa attttcattc ctagtttgtc tgacattttt
    33901 tccatatatt cttttgatgg aagccggctt tctcctttgc tgactttctt agcaattgct
    33961 ggtatggtca tcgcaaagat caagccaccg ttcacgattg ccggaagcca tcttcctcct
    34021 gcaataacta cccctaagac cgtccagaaa aaggctgacc cataacgatg aggattggtt
    34081 ttatctgtta aatttcttac tccagtgtaa atcaatacga gtcccatcac gatataaagg
    34141 atctccagta atttatcgcc taataaaata tcaggatttg taaaaaagtc catcttattg
    34201 cccctttcct ggataatatt tcttcgctaa tttcttttct ttaagataga agtagacact
    34261 tgccacaact aaagccgtga tagccactgg aacttccact aaagcaagct gtcccaatgt
    34321 tacgtcatat cctagatctt tcagtgttga ttggactagc aacgcccctg ctcctccgat
    34381 gaatagtact tggccaaaga accagcagac attttccata gctgaagcca taccttttag
    34441 ttcttcttca tgcttaggat ggacttctaa cccttttgat tcgatggtcc ccaatgccat
    34501 cggtaaaatg attggacgaa caaatccagc aactccacca aaactcacgt taaaagccgc
    34561 aaagatcatt cgaaagatcc cataaatatc tacaatcata ccagcagata ctttcttgaa
    34621 tcgcttgatc aaggaagccg ctgattcttt cagaccattt ctttcaagcg ttcctgttgc
    34681 caacataatg atgataaaaa tcgccatccc gcgattgttt acaaaacttg tacctaaaag
    34741 ttctaacatt ccttgaatgc caagaccgcc agtcaaagct gtaacaacta aagcaaccat
    34801 tacgatcaat atcgagtcta atttaaatgc aaacccgaga ataacaatca agacgcctaa
    34861 aaggctaaga taattttcca tatattttcc tccataaaat gacaatattt ttttaattat
    34921 aaagcaaaac acataaaaaa tatagttgtt ttctcacgaa atagacacta tttttaaata
    34981 tttttcgtta tttttttatt atataattca ccttacttat tgtaagataa agcttttttt
    35041 aatttattgc ctgctaatat ataaaaataa ggtctagtta ctgttttttt aaatgattaa
    35101 taaataacaa ctcctttcta tgaaattgca aagcattcat cttcttttct gctaacgata
    35161 tatccgtcaa tgcttgttac actttactaa aaagtatgag attgccgata tctggatagg
    35221 tgtgctatac tacagaaaaa gatatacgat ctgccaccgg gtagtaaaaa agaggatgat
    35281 cattccatta ggtctttgaa gggatgattc tcctcttttt gtgtattttt tatattgaag
    35341 gaggaacgta tgtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg
    35401 gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt
    35461 gattggaatg attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgcctatgag
    35521 tctctcttat cctatttgga cagggattgg cgcagttggt tcggtcatta ttggtgtggt
    35581 cttgtttcat gataagctga atctttccac ttggttcttt gtcgggttgc tgctcatcag
    35641 catcattggc atcaaaatca ctagcggtca ctagatactt agacaagcag acaaggactg
    35701 atcagaaaaa gcggggcttt ttatttgata ttcctctttc tatgaaaaaa caagaaaata
    35761 agactaatga cctagcgact tctctactca tcaggtgtat gcttgtacta tctatagaaa
    35821 aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac tttggtcaca
    35881 aacaagccct cagcgatgtc agttttagct tatctcctgg caaaatcgtt ggattagtag
    35941 gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg atccattact
    36001 cagaaggaac gatccgtatt ggtgaaaatg agatttctcc ctctagtcat aaaggattag
    36061 aacaagtagg agcattgatc gaatatccgg gtatctatcc attcttaaca ggttatgatc
    36121 atctgaagtt attctctgaa acaaacgatg tatctgctat cgacactatc gtgaaacagc
    36181 tcaagatgga aaaatacatt cacaaaaaag cgaaatccta ctcgctggga atgaaacaaa
    36241 aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta gatgagccga
    36301 tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc gacttggcta
    36361 ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa aaaatggtcg
    36421 atgagctgat cctgatcaat caaggaaaaa tcatcaaaca atgtacaatg gctgaattga
    36481 accaaacagc gcaagatttc atcgttatct ctacgacaaa tgatcaagct gctcgaacga
    36541 tcttgacaga cgctggatat cccttggaag atcctgataa aatcaagctt ctaaaaaaaa
    36601 acgaagagtt attaggaact gttatccagt tattgtctgc agaaaatgtc catgtgacag
    36661 atgtccagca tctgacgaat gatttggaat cttccctatt ggaaattctg gattcagtaa
    36721 agggggaaga agaaaaatga cgttgcttat aaaacaagaa ttatttaaat tgatcaagaa
    36781 aaagtcaact gctgttttat ctgttctatt agttgctttg ctcataggaa cagcacttct
    36841 agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc ttttcagtgc
    36901 cacatcttgg attgtcttta tcatgattgc tgctgcaagt acgatcattt caatggaatc
    36961 acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag aaatattagt
    37021 aagtaaatgg atcacattag ttatttattc tatttattta taccttttag ctattattgt
    37081 caccgtgctg atgaaactga tccttttccc ttcgatttcc tttacggaga agatttctac
    37141 tggtcagaca ctgatccaaa gcttattcac ttatactttg ggtagttata tcggtttatg
    37201 gctaatttta agtttagtgt tgatgattgc ttgttttatc aatagttccg gtgcctccat
    37261 ctcagcaggg atcgtctttt attttgcttc atcaatcata tctggtatcc tcatcgcact
    37321 cattcaaaaa tgggagtgga tcaaatggaa tccaatcagt atgctgaatc tgcaaaacca
    37381 aattggcaac gaagagatta tgaagcaatt gactcatctt tctactaatc aaatgctgtt
    37441 tggcaattta gcttacattg ctttgttctt agcattggga tacctcgtat tcaagcgaaa
    37501 aaatgtatga tggttcatta acaaagaagt tgtgacagaa gtggccagtt tcaagaaatt
    37561 tcggcttatt tttagaagaa gctacttctt gaacaccatt tattcaaaaa aagaagcgat
    37621 gacacgaatg ttgtcaccgc ttcttttttg aataaaccac aggcttcaca gcctctcttt
    37681 atacttctat acgtgaagca tcccagtggc aaataaaacc attgcgatta ctccgaccat
    37741 cgtcacaccg ataagtaaaa tcttatctaa gtggatatgt tgtacaggtt cgttttgttc
    37801 tttacgtgct ttgaggtaaa aataaatacc tggtaagaaa agaacaagaa caagcatcac
    37861 ttcattaaag gctacaacta gcatggccca tacttgaaaa gcacttgcta aaaaccctac
    37921 taaaaattgg acccactctt tccgctgata agaaagcttc atttggtaca gccctaagaa
    37981 aagccacgtg aagaaaatcg ctgctgtacc aagtgtataa gcaaagttat atgcactgtc
    38041 agtgaataat aatgaaagta aaaagacatt cgtacataac gtcgtcaata cgatagcata
    38101 agttggtgcc ccatttttgt tgagttttcc ccatgcttta ggcaaaagtc cgtcctcagc
    38161 catattcccc attgtttcaa tcggcaagat tgtccatgag agccaaaccc caactaaaga
    38221 aatgatcaaa ccgccgttga tcacgaccgc cccccattta ccaaccatca tttcaaatac
    38281 atatcccata gcaggttgcg ctttgatcgt ttgcagctga ttcatcgtca ttgtgccata
    38341 tggaagcatg gaaaccaaga cgtaaataat gatcaaaccg aatattccta gaatcgttgc
    38401 tttcccaaca tccgattgtt tttttgctcg tgaacctacg acagaagctc cttcaatacc
    38461 aacgaatacc cagagcatca ccatgatgca actcaatact tgactagaaa acgggatcgg
    38521 ctgcccattc gcattcaata tgatatcgct agaaaagtta ttaatgaatt gttggatcga
    38581 aaaggtgctg atagctacta caataaataa aaataatggc actaatttac aaaatgtgac
    38641 gatagcattg acaaagctag cattttcaac tccacgattc actagccatg catatcccca
    38701 taagaagatc gtactcatca cgatcgatgc cacattttga ccaccttcga atacggggaa
    38761 gaaataacct aatgcactca tcaacaatgt tgcgaacgcc acgtttccta accagcatgt
    38821 gagccagtaa ccccaaccgc tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta
    38881 actaaagatc ccgcctttta aatctggacg ttttttattg atattattga tcgataagga
    38941 gagcatcaac attgctgttc ctacgagtac ccagctgaag atagctgggc caccagccga
    39001 tccggagatg tccgtcataa taccaaatac gccacctcca atcgctgaac caatcactaa
    39061 ggcagcaagt ggaatcaaac caattccttt tttctgttgc tctgtatcca tgtaagcatc
    39121 ctccaaagta aaaccgcttt tatcaaaaag tataacaaac cttttttatt tttacttaat
    39181 taataaaaac tttaatagaa atataattct accgactttt ttctcattat acaagcaaag
    39241 cgcctagtcc ctttccaaat tacaccacca aaaaaactta aaaagatgct atactaaagg
    39301 gaaagaattg ggggatgatc catgaacatt ttatatatat cgatttcagg aaatacgcgt
    39361 gcttttgcca aacatctagc agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat
    39421 cctgaagtta ctttaaaaga aatccatgaa aatagtgatt ttacgaaaga aacagaacca
    39481 ttttttactt tcgttcctac ttatttagac ggcgggaatg ggttagacaa cggtgacaca
    39541 gaaatcttga ccgaaacgat gcgcgaatat ctggaatacg aagacaatca taaactttgc
    39601 cttggtgtag tcggtagcgg taataaaaac ttcaacaatc aatattgtct gacagccaaa
    39661 caatacgcac aaagattcgg ctttcctttc ttggcagatt atgaattacg gggaacacca
    39721 tctgatgtga cacgcgttta tcaaattttg gcagaaaaaa ataaataaca caaaagaaga
    39781 agctgaaaaa taaatttcgg cttcttcttt ttatgttctc tattgtttct attcttatta
    39841 agctttgaca tccatcacag gtgcttttgc tttcgcttta cctgtttttt ccaagacaaa
    39901 gttttttaca cgatccatat tggttaccaa gacgatcatg gaggtttctt tgtctgctgc
    39961 tttgattgct tctaagtcag cttctgctac aagcgtatca ggtgtcactt gctgtccttc
    40021 tgttactttc acatcgaatg gcgtaccgcc taagtcaact gtgttgatgc ccatgtgaag
    40081 caagatttct aagccatttg gtgtcgtaat gccgatagca tgttttgttg ggaaaacaga
    40141 acttattact ccagtaaccg gggaatagat atgtccgttt tctggttcta caaagtaacc
    40201 ttcacccatc attttttgtg caaaaactgg gtcacttgct ttatctaaag aaacaactgt
    40261 tccgtcagca actgatgata agatagtcac actttcagct gaagcttgag gtactgcttt
    40321 ttcaggtact tcatttactg tggctgtttc ggtcgctagt tcttcttttt ctaaacggtc
    40381 tttaggaata ccgaagaaga atgtagcaac gaagccacca gcgtaagcag ccaataaacc
    40441 aaggatatag cctaaccatt taccatcaga gatcaatgga atcaaagcgg ctcctgaagg
    40501 tccaatagcg attgctccga catttccgat cattccgatc actgccccac cgatacctcc
    40561 accgatacaa gcagtaataa atggacgtcc tagaggtaat gttactccgt aaatcaatgg
    40621 ttcgccgatt cctaagattc cgactggtaa agcccctttg atcatttctg ttaattcttt
    40681 gtcttttttg cagcggatcc ataatgccaa tgctgcaccc acttgtcctg ctccggccat
    40741 cgcaaggatt ggaagcaata aggtacgacc agtttcatca atcatttgta catggattgg
    40801 cgtcaagatc tgatgtagtc cgaacatgac cattggcaag aataatgtac ctagaacgaa
    40861 gccggcaaat gctcctccaa caccaagtac ccagttgatc ccaccgacca tgccgtcaga
    40921 aatgaaccct gctaaaggca tgatgaagaa aatttcgatc agaccgatgg caatcaggga
    40981 aagagttggc gtaacgatga tatctaccgc atcaggtact actttatgca gttttttctc
    41041 aacgattgac aataaccaaa ctgcgaagat aacacctaag atcccgcctt gtccagttgc
    41101 aagagcttca ccggtaaaca agtttttcag tggtgcttct ggattcatcc ctgtcagcat
    41161 cacaacagcc ccgataactc caccaagcgt tggcgttgca ccaaaaactt gtgcagcatt
    41221 gatacctgta tagataacta gatagctaaa catcccgttt ttcaagatgt tcatcacatc
    41281 gatatattgt tgccatgttc ctgcgtctag tgttccagct gtgactaagt ttgctaaaac
    41341 tgcggcaatc cctgccacga tcccagtgcc gacaaatgca gggatcattg gtacaaagat
    41401 attagagatg tcttttaata ccgatttgat tttagagggt ttttgtttcg atttttgtgc
    41461 cgctttcatt tcttgtgccc gttcgtttac acggtctttt ccagatgcag aagaacttgc
    41521 tgtagttgca ctagcagaag gaagtttttc tcctaatttc acgcctgcta agtcaaccat
    41581 ttcctgcgct actttgttca ctttcccagg tccgatgatg acttggagct gttcatcctc
    41641 tactacgcca agtactcctg gaatcgcttt taattccgag acatttacaa gatcatcatt
    41701 gatgaccttc atacgtacac gagtcatgca gtggacaatg ctttcaacat tgcccatacc
    41761 gcctgctcct tgataaattt gttttgccag ttcttccaaa gtcaattcgt tttttgccat
    41821 ggtcttttcc ccctcataag gttcctttta caaaaccgtt tgcttcagct aattttgctt
    41881 gagctgcctc tttatcacta tttgtcaaaa tcatgacgat agccagtttg acattttgat
    41941 ctgcttcctc aaagtacttt tcagctgttt cgtagtcagc ttctgtcgct tgcatgatga
    42001 ttcgttttga gcgttcaatt agtttttcat ttgttggttt gacgtcgacc atcaagttat
    42061 tgaatacttt accgataccg atcatactga tagtagaaat catattcaaa atcagttttt
    42121 gtgctgttcc tgacttcaat cgagtagagc ctgtcaaaaa ttctggtcct gcatcgactt
    42181 cgattgggaa atccgcatgc tcgctgattt ctgcttgttt attgcaagaa atcgttcctg
    42241 taacagcacc aatcgatttt gcgtaatcta atccaccaat cacatatggt gtacgaccgc
    42301 tagctgcgat accaataact atatcatttt ttgttaaatt cagcttgatt agatcttctt
    42361 gccctaattc ataagaatct tcggctcctt cgacggctac agtcattgct tcttctccac
    42421 cagcaattag gcctatgacc atttccggtt ctacaccgaa tgtcggtaca cattcagcag
    42481 catctaatac accaagtcta ccactcgttc cagctcccat ataaatcaag cgaccacctt
    42541 ttttaaagga ttcaatcgtg ttggcgatca ctggctcgat acttggcagt tctttttcaa
    42601 cagcagcggc cactttctga tcttctttgt tcatgagttg gactgcttga gaaatactca
    42661 tttcatctaa tccaaaagtt tcttggtttc tgcgttccgt tgttaggttc tctaaattga
    42721 ttttgctcat ttttatcttc cttcttttct tataaattga tagttcatac cagctttgat
    42781 ttggttgatc agcggtagtt cttctttgat gactttggct actacattca ctttttcatc
    42841 cgctggcaaa tcatatttag ttaattggat ctcacccata tagcgcaaat acttttcgtt
    42901 atctaaagta atagatcctt tcatacgctt agctgtattt cttactggga ttggcggaat
    42961 ctctcgaaaa cgcgcatctg cgcttcgtat gacatctctt gcatcatctt gccgattcgt
    43021 atgttttcct aatatatatt cgtaaaaaac ttcatctaca ggttccgtat ggagcaagac
    43081 tgtctgttct ttttggaaag aagcaaattg ctcctgaacg tgttctgaca gacctgcatc
    43141 tccgatataa acaatatccg tgttacttgc taatagatca agagcacctg ccagaggatg
    43201 gatccctcga tgttcttcca aagttggtaa tccttgatac agtggacctc tcaactctgt
    43261 atctcctggt acaaagcctt gtacaaggaa tccttgagtt ttgagccatt gatttttttc
    43321 ctgataccat tctttgtcca accctgtttc aagacgtgga taataattat gccaagcttc
    43381 aatctgtgag aaatcagccg ctgcttcttt caactcatcg atatcattag gcgtgatcgt
    43441 gctggcattc aaactgattt tcaattgatg tgaccagtcg gcgatctgct gattagaaat
    43501 atgataatcc attcttaacc ctgttacacc aattgctttc aagggtgtta gatcttctaa
    43561 tgaaaagcca gctcgtttca atgcctctcc tgaaatatcg accatcaact ctagtttatt
    43621 ctttttcgct tcgcttccta gttcgatcaa tcgattttga taagccgaaa catcatcttc
    43681 cggtatatgc atggaagtga aaataccgac aaagccattg gatgccattt ggcggatata
    43741 ctgtttcttc tcttctgaca aagaatcatt cataaatatt gaaaagccaa acatattatc
    43801 cctctttttc tgtatccgtc ttcatttcta tgactagagt atatatctaa agaaacaaaa
    43861 tttcaatact tttgttaaaa taattaaatt ttatttctaa aaattggatt tataatgcca
    43921 aaagaattga cacagccaca aaaaaggcac tgctaagcaa aagcagtgcc ttgaaacatt
    43981 ttatttaaat aacacaaaag tctaattact cataagaaat taattctgta ttgtctacca
    44041 tgaatgtaaa gaacccttca ttaggcattg gatgaaaata agcattgatc actcttatca
    44101 aggcattatg acagactaac aagtagacat tttcttcgtc ttccatgcat tctttcaata
    44161 aaggaacgat ccgtgcgtac acatctaaga ccgactctcc atttgggaaa cgtacagcaa
    44221 acgctaaacg cgctttttga aagttttcat ctttgcttgg cagtccgtca taatccccaa
    44281 aatccatttc taccaatcgc tcatcagcgg tcatcggcaa attgagacga tcaactactg
    44341 cttgtgctgt atctcttgct cgttgaagag gggaatggat gattttcgtg attggaacag
    44401 gcaaatctac taccttttct gctagtgcct gtgcctgcat ctgtcctttt tcagtcagcg
    44461 gcacatctgc tcgcccgcaa actaatcctt gtgcgttcca agttgtttct ccgtgtctcg
    44521 ttacataaag catgtgattc tctccttcgt tgacaaagtt ctccttaacc ataccttgtt
    44581 gtctttgaga ttacaaggtt tagattttct caagagaaat aatctcagta gatactttat
    44641 ctagaaaggt ttgatcatgt tccactagta acatagcagg tttcacgttc aaaatcagtt
    44701 gttctaattg ttcttgattg aagacatcca aataattcaa tggttcatcc catatataga
    44761 gctcagctgg ttgggataaa gatttagcca attccacttt tttccgttgt cccatactca
    44821 tctgttcgat tttattatga aaaacatctc tttccatccc aagctttcgg agattattca
    44881 aaaatgcttg atagtctact cggtttttct tcgcgaattc cgccaatgtc cctcgattgt
    44941 cttcataatt ttggcgtacg taactaatgg tcaaatgttt gggctgggat ttttctccta
    45001 caaccttgcc gttgaatgtc cccagaagat aatggataat ggatgacttt cccgcgccgt
    45061 taggacctga aattgccatc cgctgatgag gttcgatcgt aaaatgaact ggctcgaata
    45121 acagattttc ataccctaat tgaagatttt ctacgcttaa cagccgttta tgatgagaca
    45181 cttggctatt catcgtcaaa gcatcgatat actcgatatc ttttaataat ttttctttct
    45241 cactgatctg ggtttccatc cgattcacga ttgctttaga tcgtttcatc gtccgtgcag
    45301 catcagcgcc tactgctcct ttattcactc gcctagattc cgtatcgatg aatccgactt
    45361 ttttctttgt tttgtcccct tctcgggaac gagaccattc tgctttttca gctgctgttt
    45421 tctttagcct gctgacttct tttttcaatt tttcattttt agctatttca aattcatcac
    45481 gaagtttttt ctgttcttca tagatagaga aattcccttg ataaagttcc agttggctct
    45541 tttcgattgc taaaacatgg tccactactt catcgataaa tccccgatca tgactcacca
    45601 cgatgaatcc ttgttttttc tttttcaagt aagccgctac ttgttttctg ccagagatat
    45661 ccaaatgatt cgttggttca tcgattaacg ggaaatgagt gtcatccaca aataagagtg
    45721 ccagtaggac ttttgtcttc tctcctcctg atagtgtgct gaattctctc cataagattt
    45781 caggatctgt ttgcatcagt tggagctctc tttcaatttc ccatatctca aaatccataa
    45841 tctcatttaa cacataatag gtcaaacgct ctttatcttt tgtttgctgc gggaaataga
    45901 caaacgcttg ctgatggatc acttgccctt gataagggag tttgttttgc aggatattca
    45961 gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc tagtttccat tgtgtgtcaa
    46021 agtttagatt ggcttgttca aataataatg tgccttggct gtcatagccg aatgtcagat
    46081 tttttatttc gattttagac attattccat cacctcgtaa aaaaagacca gtcctatttt
    46141 cagaactggc taaagaggat gcaagggttg gggcacacaa aaagacccat acatcgaaag
    46201 aaccaagcca atcccgaaaa tctgcacact gctcccttgt ctccacaagg tacagtctga
    46261 atgcagcttt catcaatcga cttaattttt caatgcacgg atccctcact ttgtcctata
    46321 tttgaaatta cgatagcata aagaaattgt ttttgtcaac tacttcggga gaatgttgaa
    46381 aaatctgctg agtgtctgtc ctaagatttc tgttttttct aatgtttgcc tgtgttccat
    46441 tgcttcctct aaatcgattg gtcctaattg aatgcttaag acaatgggga gaaaatcaaa
    46501 gagtccttcc agctctcggt ctctacttcc gcaaatagcg attattggtt ttcgatattt
    46561 ttttgcaagc actgctacgc catatggaac ttttccgtgg attgtttgct gatcaatccg
    46621 cccttctcct gtaatgatga catccgcttg ttcgatctgt ttttctaatc cagtgatttg
    46681 gctgaccaac tcaaaaccag agaccatctt cgctcctaac aaaaaaaggg ctccgccaat
    46741 accgcctgct gctccgcttc ctgggatgtc atctaaatcc acctcccaaa catcttttat
    46801 ctgctgagca atcttttcag cttgtttatc caagaaagct aacgtcgtat ccgttcctcc
    46861 tttttgtctt ccaaattgcc aagcagctcc ttgttgacct gtatatgggt tggtcacgtc
    46921 tgcagcgatg atcactttta ctttagaaac aagaacgttc cttcgatcca aatgaagttt
    46981 tctagtagat aacaaaggat ttccttctgt gtacccttct aaagaagcac ccaaagattg
    47041 gagtaatcct agaccaccat cgctacatcc gcttcctcct aaagtgacaa tgatttgttc
    47101 cacttcttga agcagtgtat ctttgataag ctctcccaag gcataactag aagcaagtcg
    47161 gatggtttga tccgttggtt tatcaagtaa atctaatcct aatacttgtg cagattcaat
    47221 gactgcggta ttttttcctt gccagttcgt caataaataa ctgatttttc gctgacgaaa
    47281 taagagatcc atcccatcaa tcgtacgcca ggttcccccg agcgcgtaat agattgcttc
    47341 tgcagtattt tctccgccat ctgcaatcgg cacattgatt tttcttcctt tacggtcttc
    47401 ccaaccattt agaactgcct gatttaattc aaatgaagaa gcacttcctt taaatgaatc
    47461 gattgccgcc aaaatatcca tctatatccc tcactttctt cttaagtaag acactttttt
    47521 tcactttttt caaacaaaac aatgctattt tcttctgttt ttctatatac taaaagggaa
    47581 ctcattgaat aaaggagaga aaaaacatga aaatcgcaat agctggagcc ggagcaatgg
    47641 gcagccgaat cggattaatg ttgcatcaaa gcggaaatga ggtcgtcttg atcgatcgct
    47701 ggcctgctca cattgaagcc attcgtacaa atggattgat tgctgacttt aatggcaaag
    47761 aagtagttgc aaaactgcca atctattcac cagaagaaat catcgaaagc aacaaacacg
    47821 tggatttgat cgtagccttg acaaaagcga atcaattaga tgatatgttt cgttcgatcc
    47881 aatccattat tactgagaat acttatgttt tgtgtttact gaatggatta ggtcatgaag
    47941 atgtactgga aaaatatgta ccaaagaaaa atatcttatt tgggatcacg atgtggacag
    48001 ctggtctagc tggtcccgga aaagtcacat tattaggtga tggagaaatc gaactagaga
    48061 acttggaacc agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt caagaagcaa
    48121 aattgaatcc aatctatagt cataatgttc gttattccat ttggcgaaaa gcttgtgtaa
    48181 acggaacatt aaatggatta tgcacgatat tggattgtaa tattgctgaa ttaggtgcgc
    48241 aaaaagcggc agagggcatg gttcgaacga ttgtatctga gtttgcttct atcgcagcaa
    48301 aagaaggaat cacattagat caagaagaag tgtaccaaca tattgcttct acttatgatc
    48361 ctgacaacat cgggttgcat tacccatcta tgtaccaaga cctgatcaaa aaccatcgtt
    48421 tgactgaaat cgactacatc aatggtgccg tttggcgaaa aggacaaaaa tatgatattg
    48481 ctacaccata ttgtgccttt ttgacccaat tagtccatgc aaaagaagga atccttggcg
    48541 cagaataaaa ggctctctga tgagaatcag ctggatcagc agctggttct cattttttta
    48601 cccccatgaa agttcaaaaa gaaaaacaca attctcatgt tgccataaga attgcgcttg
    48661 tttattttca tctatgattg gttggcactt ttatttattt ttaacagagg aaagatcgta
    48721 ttcatattgt tcataaatag cggagtcagt agtgaaaaaa cgttttgccc aattttctgc
    48781 atcaaaaaat tccaacagtt ctgatgggat tggttgaaat ggcgtatgca aaaattccgg
    48841 taattcatct aaatcacgtt cttgaagcaa aaaaatattt tcagaatgat aaaactgata
    48901 atgagctatg gcttgattat ctgtcactag tttcttttga aaatacatag attcaaacgt
    48961 tcgcaaagtc actccttgct gcccttcccg caacagttct agaatccccc tcgattcccc
    49021 ggtttttttc agatattctt cataagtaag gtatccaggt tgttcatttc ctcgaagcgg
    49081 aagaataaag taattggtcg taacacctct ttttgtaaat tcctgcttat atttttctgc
    49141 ccgttctttt ctgccgttat ttgttgcacc aaaaaataga tccgtctttg gttcgctaga
    49201 agaaaaatcc atatgtttcg aataaaagga actattatgt tttagtccaa gttctttcgc
    49261 ttctaaaaaa tcaaaatgat aaatctcatc gattgccggt tcatcttgtg cctgcttcag
    49321 catcgcctcg tgttctgctt taaagtggtt ccaaaaaaat aacacgattt ttgcattggt
    49381 aaatggacgc acgcgtcgag ccatctccat atcaaatcca taatctaaaa aaacgatcag
    49441 ctgataattt gccaactgtt gtcgagctga tttaatgaat tccgactgta gtaaatcttt
    49501 tccggaccaa acatctaaag agcggatgga aggaaaaaaa taaagtgatt tatttttcgc
    49561 aattagcaat acgtcgtttt tgttcatctg ttccgcctca ctttctctgt cactttttcc
    49621 tatgatagcc tgtttaaata gtaaaaagcc agtaaaacgc ttcctattat ttttataaaa
    49681 atttaacatt tctatgaaaa tacgatgaaa taaatcattc ttccattaaa atatgcccaa
    49741 aagttttctg aatagttgta attatctgaa aatttgttat cattagtcat tattattatt
    49801 actatttatt cttactattt attcttacta tgcattttga ttgtgaattt aaaagaaaga
    49861 ggttatttat ggaagataaa aaattgaaaa aacaacttag ctcccgacat atcaccatgc
    49921 tagctttagg tggagcgatt ggtgcaggtc tatttaaagg aagtggcgaa gcgattggaa
    49981 tcgccggccc ttctgtattg atcgcttttt tgattggcgg cataatttta tatatcgtga
    50041 tgaaaggtct gggaaaaatt gttttaagtg gtggtgatac gcaccacgga ttatctggat
    50101 tgattcgtcc atatctaggt gctcatgctg ccgattttac cgattgggtc tactggtcca
    50161 tgtggattat caatatcatt gctgaagctg ttgcagctgc ttccttttta cagttgtggt
    50221 tcccccacat cccagcttgg gtatttgttt ttatgttggc tgttttaact actatcatca
    50281 atctttattc ggtacgatta ttcgcagaga cagaatattg gctggccttt gctaaaatca
    50341 gtgtcattat cctattgatc atttttgctg cttatcttgt cggtcagcaa atgttgggta
    50401 ctggtgtttt ccctaccctt caacaattaa ctgatcatgg tggttttact ccccacggta
    50461 tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt tctgagttga
    50521 ttgctatcac agtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag
    50581 gcgtcatggg aagaatcatt tccttttaca ttattccttt atttttgctt ttgattattt
    50641 ttccttggaa tactttagca ggcacgaacg ttagtccttt tgtgatggta tttgaaaaaa
    50701 tgaatattcc atttgcggca gatatcgtca atttcgtgat tgttcttgct ctgttctcgt
    50761 ctatcaattc aggtgtatat gcttcttcga gaattttgtt cttccgcttg aaagatcgga
    50821 aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa cgtgctgtgc
    50881 tgttttgtac cagtgttctt tatctaggtg tggctctttc ttattttgtc ggtgacaaat
    50941 tatttggcta tctggcaggt tctctttcct atacagtatt attgatctgg atcattatca
    51001 gtgctgcgtc ctttgtactg acactaagga aaggaacgtt atgggacaaa ggaatcagct
    51061 tttttgcatt agcagtctta tggttgattt tccttggtat cctattcacg aactccattg
    51121 gggtaactgt attgaccggt ttgctttacc tgttcattta tttcagttat caaaaaaaga
    51181 atgatgcttt cgtcttgacg aatgaatgaa accaatagag aattttattc tatttatatt
    51241 gaatatctgg agctattgaa aaaaaggagc tgcgacaaaa attgttgcag ttcttttttc
    51301 cgaataaacg gtgttcaaaa accatctgtg cagattaatt gattgttctc cacgctcatg
    51361 acatgataga agaaaggaat gaagaaaaag gagtttgttt taaatgaaaa agtattggaa
    51421 gtttttgttg atgattggtg tttcgacggt agtcatgttt ggtatgatgt atttgaatgt
    51481 ttatgcgctt gatcatatct tcttcagtcg cacacgtgta ttcatggcgt tgatgatggg
    51541 tgcagtcatg gcagtcatca tgttgttgtt catgtggaaa atgtatgaaa acaaaaagat
    51601 aaatgctatg attttagccg ctagtcttct tgtttttgcc ggttccttat ttatggtacg
    51661 aagccaaaca actgtcaacg atacggcgtg gatgaaggcg atgatccctc atcactctat
    51721 tgctatcttg acaagtaaac gagctgattt aaaagatcct gaagtaaaaa aattagcgga
    51781 agaaatcatt caggcacaag aagaagaaat cgacaaaatg aaaaaaatga tcaaagaaat
    51841 ggaatgacca ttatccatcc aaataaaaaa agaggatgtt ccagaagtga tcaacttcaa
    51901 gaaataagaa gaagctactt ctggaacacc gtgaatcaga ggttgtgaaa aagctgccct
    51961 gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttagg
    52021 cttattaccg caaatgcaag cttttgaaca ccgtttattc gtaaaaggga gtgtgacaaa
    52081 agtcttgtca cactcccttt ttacgttttt attgttctaa ttgcgctgtc ttgcttactg
    52141 cttcttgcgg tttcacaaat ttctttgaga tcccccaacc tgtaaaggca ctgaccatcg
    52201 aaaacacagg tgaaagcaaa ctgaaaaaca cgaatggcaa gtaagtgagt gtaggaatgc
    52261 caaatgtact tgctacgaaa cttccggcga ttccccaagg cactaagtag ttgattactg
    52321 ttccaccatc ttctagtgtt ctccctaata cagttggatc aaatcctgct ttttcgtaag
    52381 tttctttaaa tgcattacca ggcaggataa cagatagaaa ttgttctcca ataaagatat
    52441 ttactccgat gcttgtcatc aatgttgtaa aaatcaattt tggggtgttc gtcatctttc
    52501 ttgatactcg ttccataacg gttgagatca acccaaattc gatcaacaaa ccacctaatg
    52561 acaatgttag aataatcaat gacacagttg gcatcatgct ttcgattcct ccacggctaa
    52621 gtagtgaatc gatttgttgg tttccagtat ttgaaacaaa tcccgattcg atgacccctg
    52681 ccaacttcgt taaggaagtt tgtgggtttt gaataaagat cattcctact gctacagcaa
    52741 tattcaacaa aatcgtcaaa attgccggta cctttttcca tgcacacaag agcattaaaa
    52801 atagaggaat cagtgaccag atcgaaatag taaagtttgc ttctaaaata tcagtcactt
    52861 cacgaataga tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg aataaaaaga
    52921 aagaaacaac aaatgccgga acagttgacc acatcatatg cttgatgtgt ttgaacaaat
    52981 cggcatcaac aatagctgct gctaaatttg ttgattcgga taatggagac atcttgtctc
    53041 caaaaacggc acctgaaaca atggctccaa caacaagggc tggatttagt cctagtgttg
    53101 tcccaatacc gaaaaatgca atcccaatcg ttgacataac agtaaaggca ctccctacag
    53161 cgctacctac gattgcacaa acaacaaata cagaagggac gaaccactgg acactaatca
    53221 atttaaatcc aatgaccatg agggttggaa taattcctgc ttgtatccat acagcaatca
    53281 atgaaccgac taaaataaaa ataaagattg gaatgatccc cggtttgatg ccattgatga
    53341 tcccttcgtg cagccaatct gtcgggtatt tttttatcac tgcgtacaac atgaccattc
    53401 cgatagctgc caaaatcgtg atattaggtg aaacctttaa tccgacaacg ccaatcgcaa
    53461 tgcctaaaac aatgaccatc aatacaagtg ttgcttctaa aaaacttggt gttccttttt
    53521 tttccatgat aatctctcct tttttagttg ttcttatgtt ctaaaaaagt taagatatcg
    53581 gattactccg ccacaattca tccgcgattc ctcctttata aaaataaaaa gacctgcttt
    53641 caactaagga cgaatgactc cgcggtacca ccttaattaa aaacagatct gtttttcact
    53701 ttcaaacgat tattcaaatg tatggacttt ccatgtaatt cgtcaattag gcttgatcgg
    53761 ttcacagcga ccaccgactt tctgaacaaa acacctaacc actactgcgt gaaaagttga
    53821 cttgtatgaa gtttaccttc cataagatca tttgtcaatg aaaaagaaaa tattttttca
    53881 tttttctgaa aattattttt ataactttcg cgcacaactt tatcttttta ctagaaagta
    53941 gcgccctctt gtttttttct tgctactatt tcttgatgtt cttgtttgat tttttctaat
    54001 agcttgggtt ctctcaataa atctagagct gtattggcaa gtaattccgc tgcaatcgca
    54061 atagaagcaa gtcctttttc acttttagca gcagctttga attcttctgt atgtccggca
    54121 atatatgtct cagagattgc tacagtaggc tgtatcgttg gaataacttg gctaacattt
    54181 cctacatcag aagaacctag acttgttctt tgttccgttt ctatttcttc atcaggaacc
    54241 cctgcttctt ttgcatgttg gaagaataag tcatcgaaag aaggcgttac gatgacatca
    54301 tcgactgcat tttggaacaa tccaaactca aaagtcgtac ccgtgcttaa tgcagcacct
    54361 cgtacgatat tttccacttt ttcatatact ttgtctaatg tttgtcgatt tcctgcacgt
    54421 aaatagaaac gtccagcagc gtagtctggt atgacattgg ccgccacgcc tccatctgta
    54481 atgattccat ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccgtta
    54541 aacacttgga tcaaggcttc caaagcattt actccttttt caggtgctgc tgctgcatga
    54601 gaagcgacac cgtaaaattt gatatcgact ggatcatttg ctaaagattc tgtcgttttc
    54661 ccataacgaa aagctggatg gacacataat gctgcatcga catcctcaaa aaaaccttcc
    54721 cgaacgaagc ttcccttagc tgatccattt tcgcctcctt cttcccccgg agtaccatac
    54781 acacgaattt ctccgccaat ctcatcaatg aattgtttca ctatgctggc tgctaatacg
    54841 gagtaagtac caaataaatt atgtccgcat gcatgaccaa tacctggtaa agcatcaaat
    54901 tctgccaaaa aggcaattgt cggacctggt ttattggaaa tataacgtgc atcaaagcct
    54961 gttcggtgcc cagctacttc tttttctacc cagaaccctt ctttttccaa ttgtttgatt
    55021 aaagctgctg aggaataaaa ttcataatta cttacttcag gatggtcgtg gatagctagt
    55081 gcaagtgctt gatattctgg taaacggtat tgaatcagat ccttcaattg atttgttgag
    55141 agtgtggttg tcataaaaat ttcctcctat gctagtcgtt ttttcttatt ttctcccgaa
    55201 tgtttcccac gcaggtacgc ttgaaccttt tgatgttttt tcgattactt tggctgtttc
    55261 ttctgtttga taggcatcta ctattttttg atacgtttta ttgtcttcat cttcttttct
    55321 tgcaacgatg atattgacat aaggacgtgc atgctcatcg actggttcta agaagattgc
    55381 atcagaagcg ggattaaatc cagcatctac agctacaccg ctattgatga ctgaaatatc
    55441 tacatctgac aaagcacgtg ctgtttggga agcatctaac tcagaaatgt ctagattttt
    55501 tttattctcg gtaatatcag aaacggtagg cgcttggcct tttgccttgt ccactttgat
    55561 caatccagca gtttgcaata acagaagagc tctgccgcca ttcgtgacat cattagggat
    55621 tgcaactttt gctccatctt tgatatcagc cacatctttt aacgtattgg aataaattcc
    55681 caacggtgca ttaaccgtat tcccaatcga tactaaatca gttccgtgtt cttcattgta
    55741 attgtctaaa aagaattgat gttggaagga attcaaatcg atcgatccat ctgctaaagc
    55801 cgcatttggc tgattgtaat ctgtaaattt cacatattcc aaatggatgt tttccttttt
    55861 caaacggtct ttcacatcgt cccatacatc tgtatcttcc ccaatgatcc ctagttttac
    55921 ggtcgtttct tttttttcag ttgtagctgc ttttccttgg ctgcatcctg ccaaccaaat
    55981 aattgctaca aatgtaatga ctgcaaaacg aataattttt ttcatttttt ctcctccatt
    56041 ttcaatgtgt gactttttta atcagttgat tactgataaa ttgactcaaa aaaactaaga
    56101 ccagaatcaa taatgtagcg acaatcgtta catctgtttg gaatcgatta tatcctctcg
    56161 atatagcaag attccctaat cctccggcac ctactgctcc agccatggta gtcagtccga
    56221 tcacattgat gatcgtcaac gctgagaccc gaatgatacc aggtaaccct tctaacaaat
    56281 aaacacgtaa aatgatgccg attgggctcg tgcccatcgc ttctgctgct tcgatcaccc
    56341 caggatcaac ttctagcagt gcattctcga tttgacgggc aaaaaacggg acaacgccaa
    56401 aaatcaaagg gactaaagct gctttggcac caatcgtcgt tccaactaag aaacgagtag
    56461 tcaaagctaa aagagaaagc aagataatga aagggatgga acgaatgata ttgatcaact
    56521 tatcaagtat ttcgaacagc agcctgttag ctagtaatcc atttggacgt gtcactacta
    56581 acgtcacacc taagatgatt ccgatgatcc caccaatgaa tgccgtccaa aaagacatat
    56641 atagtgtttg ctttgtcgct tcgatgaatt catccggaat caaatagaca ttcggcaaat
    56701 atgttttaaa aaattcagtc attttagttt cctccttcga tgactttgat acggttatct
    56761 aattcatttt tttgtaaaat agttactttt acaccagatt taatcaaata atccaatgct
    56821 ttttgccgct gtttggtttc tccagctaaa gtaacgatca aacttcctaa gggtgtttct
    56881 tgtaatagct ctacattacc gtataagata ttcgctgtta cttggtactt gctgtaaaga
    56941 tgagcgatta gaggttcgtt cgtctggttg ccgatataac ttaattccac caaccattta
    57001 tttgcaatct gatcggcaaa agcagcatgg gcactgattg tttccaatgc ttggtcaagg
    57061 tgtgtcgctg tccgaataaa atctttagtc aattcttctt caggatgact gaaaatctgg
    57121 atgctgctac ctttttcaat gattttcccc gcttccataa ccgctacctt gttgcagatt
    57181 tctttgacaa cttgcatctc atgagtgatc aggacgattg ttagacctag ctgacgattt
    57241 agttttttta acagtgcaag gatctgaagg gtcgttttcg gatcaagtgc actagttgct
    57301 tcatcgcata acaacacttt aggttcattt gccagagctc gtgcgattgc cactcgttgt
    57361 ttttgccccc ccgacagttg tttaggatac gcagatgctt tttcttccag accgacaagt
    57421 tccagtagtt cattcacttt ttgacgacgt tcctgttttg attttccaga atactttaga
    57481 gaaaaatcta cgttgtcaaa aatcgttcga ctttccatta gattgaaatg ctgaaagatc
    57541 ataccgatgg cctttctttc ttttcgcaat tcttttggag agagctggct taaattcgtc
    57601 tgattgacga tgacttctcc tgctgttggt ttctgcaaca aattcatcac gcggactaac
    57661 gtactctttc ctgcacctga gtaaccgacg atcccataaa catccccttt ttcaatcgtt
    57721 aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca tacagaaata
    57781 tctcgcaatt caatcattaa cattcctcct tgtctataaa aaaagcatcc tcaagaaaat
    57841 gaaaatagtc attttcttga ggacgccttt ttgcgtgtta ccacctcatg ttcaagctgt
    57901 gttcacacac aactcctctt caagtaccat catactctag caagatatcg gtcgcagccg
    57961 tcaccgcctt acatctatgc tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt
    58021 ccttgcttct tctcagctac cgaagctctc tgtcaaaggt tcttctatcg tacttaccat
    58081 ttcaatgcaa ataatattca aaaaaaaaat ccttttacta acataaacta tgtcagtaaa
    58141 aggacgacgt atcgtggtac cacctttttt cggaagtttc cttccctcaa gcccaacatc
    58201 tcaaaaagag agattggcgt tcgttttaac ggtcgaacat ttccgaattt gcctacttat
    58261 cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc acaccagcca
    58321 gagcctctct gcaaagtttc caagcttact ttccttctca acgaaatcaa tattttgttg
    58381 ttgggaataa tctacaggag gaaaactgaa aagtcaagca tgaaatccaa attatttttt
    58441 ctatatttat ttttctgttt ttttatctaa catttcaata acagttcgaa gatcttttcc
    58501 ttccgcaaga aggttcaatt tttccttctc ttcgacgcta taatttcgaa caaaactgga
    58561 atcgggaatc aaaacaacgc ctagtcctgc acgtgaagcg gctgtcgctc ctactaaaga
    58621 atcttccact gcaaaagctg aaagagcttc taatcctgtt cgttccaaag ctgtcagata
    58681 agggactggt gaaggcttgt tctccgatac ttcatctccg aacgtaaacg tatcaaagta
    58741 atcggctaat tgaaagtgat ccaagatatc tgttgctctt ttcgtaacag ttgtcgtagc
    58801 aatccctagt ttcatatgac gttcttttaa gtctgtcaat atttcttttg catagggttt
    58861 caattggata gctccacttg tcagctgttg ataaaaatat tcttcacgct ttgctcggac
    58921 ttcctgcact ttttcaggac tgcctgctgc ctgcactaaa atatcaaaac tttgtttcca
    58981 gttttttccg ccccaagcgt caacgatttc tttcgggata tgaatatcaa attgctggaa
    59041 tccccaaagc caaccatccc gatacgttct ttccgtatcc actaacaaac catctaaatc
    59101 aaatattgca cctttcatct atacattaat cctttccaaa acaatacacc tagtttagca
    59161 tgactgatcc aaaatgaaca gaagcataga gcccagccgc atcacttata agagttcttg
    59221 gaatcgacgg atatagattt tcttcacagc tgtcacaagc gccagataag cgacgactgt
    59281 tataaaaaac cataaccagt aattgccagg gagcggcatc aagcctaact cagcaccaaa
    59341 tcctgtgaat ggaaggactg aaccgacaat gattcctaat gtagtgataa ccagcatggc
    59401 aaatgttgca ttgctttgga taaatggtat tttcggtgta cgcaatgcat ggagtactaa
    59461 tgtttgtgtc cacagggatt cgacaaacca tcctgcatgg aataatgcga taaaaatcac
    59521 ttgttgtgag ctatccaatg tatggaaact tccaccaact acagcaggac aaataacaaa
    59581 atacatcaac aagtaagtag tgatatcaaa aatcgaactt gttggaccaa aataaaccat
    59641 gaattttcca atactagatg cattccattt ctttggctca tccaaatatt ctttatccat
    59701 ccgatcccat ggtaaggaaa tgcaagaaac gtcatagatc agattcaaaa atagaagttg
    59761 caaaggcaac attggtaaaa atggtaagaa cgtactggca atcagtacgg aaaacatatt
    59821 gccaaaatta gaacttgctg ttgccttgat atatttcatg atgtttccaa aagtttctct
    59881 tccagaaaga aggcctcttt ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc
    59941 tgattctttt gcgatatcca cagctgtatc cacggaaata ccgatatcag ccactttcat
    60001 tgctggagca tcattgatac catctcctaa aaatccgaca gtatgcccat ttttacgtaa
    60061 agctgtcgtc aatcgtgctt tttgctgagg attcaatttt acgaatactt cgtgttcttc
    60121 agctacttgc gctaattcat gatcattcat ttccgagatt ttctcaccag taatcaattc
    60181 attgatttct aggcctactt gacggcacac agaccgtgtg actaattcat tgtccccagt
    60241 cagaactttt acagctacac catgttcttt taaagctttc aacgcttgtt ttgttgtttc
    60301 ttttggtgga tcaaggaaag caagataacc aatcagaacc atgtctgact catctttgac
    60361 tgaaaattct ccaacaacgc ttggattggt cttttgtgcg acaccaatca ctcgtaagcc
    60421 gtcttcatta agtgattgta cttttgcaag caccgcttct tttcgttctt ttgttaatgg
    60481 tgcgacagta ccaccaagat caataaattt agaaatgttc aacatttctt caatcgcgcc
    60541 ttttgtgatc atctgtgttt tgccagccgg atcagcgact acaacactca tacggcgacg
    60601 ttcaaaatca aaaggaattt catctacttt ttgataattg attgtatctg tatctaaagt
    60661 ttctgctgct tcatcaatga tagccacatc taacaaattt ttcagccctg tttgatagta
    60721 gctgtttaga taagcatggc gcaaaacacg atcatcttca tttccttcac aatccatatg
    60781 atattcaaga atgattttgt cttgtgtcaa tgttcccgtt ttatccgtac ataacacatc
    60841 gatcgcacca aaattctgga tagcatttaa atttttgatg actgttcctt ttttggccat
    60901 cacacttgct cctttgacca aattcgtcgt aacgatcatt ggaagcattt caggcgttaa
    60961 accaactgcc acagataatc caaaaagaaa agcttctaac caatctcctt ttgtaagccc
    61021 attgataacg ataactgctg gagccatcaa tgacataaat ttgatcagca gcatggatgt
    61081 tttgtggatg cctaattcaa aacttgattg gatgggtttt tcagatagcg tttctgctac
    61141 atgcccaaat agtgtgtcat ttccgacaga aacaatgatt ccttccgcgc ttccactgat
    61201 gacattgctt cccataaaga caagattttc ataacttgtt tcactttgct gatcgtcttc
    61261 acatactttc gcatgctttt ctactggata gctttcccct gtcaatgctg cttgagaaat
    61321 gaatagatcc tttgcggaca ataaacgcat atccgctggt atcatatctc ctgctgaaag
    61381 ccgaacaaga tctccacaga cgatttcttc cataggaatc tctgtatact cgccatttcg
    61441 tttgacagct gccgttactt tcacaagtga tttcagtttt tcagctgctt gattagaacg
    61501 cacagactgg atcaacgtca tcgtcccact gacgataacc atagtgaaga taatggctac
    61561 accaaagaga tctttatctc ctggagcggc taatacgtat tccgtaatga atgaaataat
    61621 tcctaatgca atcaacacta atgtaaaggg tgtgatgtat gctttcaata tttctataat
    61681 gaatggtgtt tttttgccat atgaaatcgc attttcacca taagtttctc ttgaacgctc
    61741 gatttgttct tctgacaatc catttggtga tgcatggaac aattcaaata attcttgctt
    61801 cgtttttcca gcaaaagctg catagatttt ttgtgtatct ttcttttttt ccatctcgat
    61861 ctctcctttg tgtccaaaag acagctgttc tcgctgtttt tggtcttttg ctattcatcg
    61921 gatcgataga gaaaatgctg aaaggggctg agaacagata gataatagcc ctggcctttc
    61981 ctctaaatcc ttgtttttcg aatgactgtc atccatttgt tctcatctcc tttactaagt
    62041 aattttaatt catgacttcc aatcattttt cgaatagtta ttttcccaac tcgctcacct
    62101 ccctttttgt tttttcacct atgggatttg cggcaacaaa aaaacctctc gccaaatact
    62161 cagcgaaagg tttgaaaaag caaatcaaaa aaagcttctc agatctcatc gttgagtttt
    62221 gacactgtac aacgtaaaaa gcagatatct gcttttagct accttatgaa aagccttatc
    62281 tcgacaattc ctgttctacc cattggcgtc tctcgacgtt tttgggcagt agcctatgtt
    62341 tgtataggag cctcacctaa caaatcctat tcggttttcc ctcgttaata taacaactaa
    62401 aaaagaggat gtcaaatcaa ttttccatgt ttttcatact tttttaaaat acacgcgtta
    62461 tatttcctgt ctcacgcagt tttatttgtc tcattttcca cctcccgcta taatagaatt
    62521 atagtaggaa acgaggtgct gttatggaac gaacacttgt tattatcaag ccagacggcg
    62581 taagacgcca tttggtcgga agcatcatcc aacgttttga agcaaaggga ctggccattg
    62641 ctgaaatgaa atttgcaacg atgacacctg aactagcaaa agaacattat cagcacttga
    62701 ctgaacgtag tttttttgat gagcttatcg attacatgac ttctggtccg gtcgtttatc
    62761 ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa gatggtcgga gcaacaaaag
    62821 gggcggatgc cgttccaggt acgatccgag gagattatgc actgccgggg actgaaaaca
    62881 tcatccatgc ttctgattcc cgtgatgctg caacaaaaga gatcgcccgc tttttcccgc
    62941 cattcgatac aaacaaaccg tctgaaaaag cggcgaagtg atacaaatcc taaactgttg
    63001 gtctaactgc tgggtttagc aatggccagc agttttttct ctaaagaatt cctttgattc
    63061 ttccctaatg tatattcgat cactgcgaaa aactcttggt gattttccga tttttcttcc
    63121 cctaaagcag cagaaatcag tcgttggtaa taatccgcca tcaatgtttc ataaatcact
    63181 gtacttcttt ctttatgcgc taaactccgt ttcatatctt cattcacgct acgtgccttt
    63241 tttaagcgac cattttctat ataaaacaaa gtcaagttcg tcaaaaaagt aaagagatac
    63301 tgctcgtgat tattcagttg cgcgatccgt cgcattcttt ttttgatttt caaataatta
    63361 ttctcaataa aacgatcact gaatatccaa ggaaaatctg ctagaataga tacttcaaat
    63421 tgcccatact ctggtacttg catcaaatat tcttcaatga tacgacattc ataacgcaaa
    63481 aattcaaatt ccgtctcaga tacttctgtt tttttggaca tgtgcagatt caaaaccaaa
    63541 tagtacagat cttctgtttt cttatagtgc ctgcggttat tagcaatttc tttgtaagga
    63601 tcaaagctgt cttcttgaat ccgatctagc attcgtcttt ttttctttaa tttttttggc
    63661 tcatcaagct gatttcgtat ccattcatat tcggcaagtg tcatgttcat tcgctctaaa
    63721 aatgatttca atgttccaaa cgatactttt ttcactgaat ttgtctcgat cccgactagc
    63781 gaagttttag aacagattcc ttccgctaat tgggtctgtg tcagcttttt gttgactcga
    63841 agcttcttaa tcaattctcc atcatacata atagttctcc ttttttaatt gaaattaacc
    63901 cttggctttt ccagaatgct tgccaataag aggaatgatt atttcaattt aaattttccg
    63961 cattcaataa atagatacgt taaaaataaa aaatacacga aaaaaataaa aaaatgtttt
    64021 tctcataaaa aataccaacc ttcaagcatg agactgctgt ctaatccctg aaggttggtt
    64081 tgattgttta ttgatcattt ttttactgct ccgcattggt tcctgttact ggatatagga
    64141 tagtcgttgt tttattgccg ttttttgaaa agacactggt cgaatgttca cctttttgct
    64201 tttcgattgc ttcattacgt tccgcttcgt ttttatagtc atattttttc ggatcaatcg
    64261 tctcaaatcc gtttggcaca taaaaccgca agagattttc ctgattcagc ttatctgaaa
    64321 gacttaacgc agtcttgact ttttcctgat ctttggagat ttcctcgtcg attcctgctg
    64381 ctttcgtatc gatgatttct cctgtctgat tattgtaagc ttttcctcct aggacggtgt
    64441 atttgggcgt cacgaagttc ccatttcgaa aagcaactac ttgatcgtgc tgagaagata
    64501 ataagtctgt accaaaatga agataatctt tatcatcgat tcccaataga tgtagtaatg
    64561 tcggcaaaac atcgatttct ccaccatatt catgattgac tgtcccttta gtgtaaccag
    64621 gcatatggat catcaatggc actcgttgca tctgtgcgtt gtcaaattca tcccatgtat
    64681 ctggatcttt accaagagca cttgctaaat ctttattatc tgtattcgag atcccaaaat
    64741 gatccccgta aatcacaaag attgaatttt ggtaaatacc agattttttc aaatacgtaa
    64801 aaaattgttc caatgattga tccagataat gggccgttcg aacataatta tctactgtgc
    64861 tattgccagt attcaaagat ggaaagtcga aattcttgtc atccgtataa taaggcgtgt
    64921 ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg tagatgttca agatattggg
    64981 cagattctct gaataaatct ttatctaata tcccataacc taacgtttca tcggattcat
    65041 caaaataaga tcgatcaaag aaattgtcat accctaaatt tttatataca tgatctctat
    65101 tccagaaact agctacattc ccatggaaaa cagcacttgt atagccttct gtttgaccaa
    65161 gggtcgctgg tgctgcttga aagacattat ctgacccaag ttctgtgaat aatgagcctt
    65221 gaggtaaacc aaatgtgcca gtttccaaca tgttttcagc atcacttgtc tttccctgtc
    65281 ctacttcatg aaagaaattg tcaaaactga tcgttgcttg attctggaaa atactattta
    65341 aaaacggcgt gacttcttgt ccgtctacct tcatattgat caaaaactgt tgaaaacttt
    65401 ctaaatgaag gacaatcaca ttttttcctt tagcaatccc aaatgtttca ggatttggct
    65461 ctgcataatg tttctttgta tagtctaaaa cttcatcaat tccattactg cttgcatgcg
    65521 cgcgaacact gctagtcatt cctgttttga ttccgtcata aatagtgtac gcatcgattc
    65581 ctagatattt gacaatatag gaacggtcaa atgtccgctg tagcaattgg ggacggttgg
    65641 cttcgctcaa cgctaaattt cctgaaaaaa tcaatcctga aagacaggta acagcgatag
    65701 ccatcggttt gctgacaggg ttgcttttga taggtatttt cttgaccttc aaccaaacca
    65761 gtagcccaat gaacacagcg atatccaacc aatagatgac atcatggggt ttcatcaaag
    65821 aaaaggagct tcctgacaac ccttgtgaca ctttcgaaaa gcctaataca gatttgactg
    65881 taatgaaatc tgtgaattcc cgataaaaaa taatattcaa gtacaacaaa agcgtattta
    65941 aaatatcaat gatcaaaaga atggctaacg ctggctttgg ctttttaata tacaaagaaa
    66001 ggccaaaaaa caataaggta gtcgcaatcg ggttgatcca caagataaag tactggatcg
    66061 taccttccac acctaatgaa aaatctgcat agtaggcaat gatcgtcttc agccagaaaa
    66121 agaaaacagc tgtcaaaacg attgctaaac gattttggca caatttttta agttgtgtca
    66181 cgtatatcac ttctctctat tcacattttt tcttattttg ctcctgaaaa atgaaaattt
    66241 cctaaaaaag ttgttatctt aagatttttt tattctttct aaagattatt ccattcattt
    66301 ttcacagcat aaaatcgttt cggtgtaagc ggttaaaata gtaaggagat aaaaatcttg
    66361 tggtgaccga tttactctat agtattcaaa agtagtttct cggtattcgt acaaaaaaaa
    66421 catactaaga ttagagggga acatcattga cgaatgatgt tctcctctat tttctatttt
    66481 tagattatag tgaagagtga aagaaagttc gaacaaagcc ttgaggacta cgagtcccta
    66541 tcaaaaaccg aacttcttta ctgtcatttt agaatcaggt ttacgtatgt tggggccatg
    66601 agcccgtgta taggaaattg gaatcgaaat gaagcagtaa agttctttca acacttaaaa
    66661 aggaggaaaa caaatgcctt acattcttgc attagatgtt tcaatgggac acagctacag
    66721 tgtcctatac aaagacgaca cctgcttatt tgaagacgag atcgaacaca ctcaaagagg
    66781 atttcatgca ttactcgaag agatccagca cttgcctgaa cgtccattga ttgtttttga
    66841 agcaacgggt atttattcca gaccagtcga aaagttcttt caagatcatc acttttctta
    66901 ctgtttgtta aacccgttag aagcgaaaaa acagatggaa gaaaacacct tacgcagttg
    66961 gaaaacagat aaatcggatg cccatcggct cgcacagact catcttaaaa atcaacgtca
    67021 accaaaagaa gtacaaagca atttttactt agagatgcgc gatttagccc gtttttatca
    67081 agaaattgag aagaaaatca ctcgactgcg catggattta cacaactgcc ttcagttgac
    67141 ttttccagag cttgaacagt tcttttcaaa caggctgacc ccttacgcat tgacattgat
    67201 ccgtcttttt ccccatccag attttgtatt ggcatctact cggacaaaaa taaaaaataa
    67261 actgatcaac gaaacacggg aaaaaatctc cgcgaatcgt gccgagcaaa aggcagatca
    67321 aatcatccac tatgcacagt gtgcctatcc agctgttgaa aaagacagta ttcactgtca
    67381 aaaaacgatc tattacgccg aacttctcca agatttactc gaacaaaaaa aagcactggc
    67441 gacacaaatg atcaaaaaag ccgaagcctc tccttgcttt ttgttgtacc aaacgttccc
    67501 tgggattggc gcactcacag ccgctttatt gctcggtgaa ttaggagata tcacacgatt
    67561 taaaacccat aaacaattga atgcctttgt cggaatcgac atccgacggt atcattcagg
    67621 taagtatact ggccaagacc gcatcaataa gcgtggtaat cccaaagcac ggaaaattat
    67681 tttcttcacc attcgaaata tgattcgcca gcaacgcgca gctcctaatc atattgtcga
    67741 ttattactac aaattaaaaa agcaacctat ccctaagaaa gataaggttg ccacagttgc
    67801 ttgtatgaac aagcttctga aatgtatgca cgctatggtt agggcacata cagagtacga
    67861 ctatgcgtac gcggtctcag tggaccatta actaaaccac ttacagtata ccacttcttt
    67921 gcccagatcg aaaatttttt acgaacgggg ctttatttag tgtgccttga ttttgtcgct
    67981 ttcgtttacg agctattttt cttgcacctt ttacttgact aatcgtagga aagaggctgt
    68041 gacaaaattc ttgtcgcagc tccctatttc ttgatgcagg acagcttttt cacaacctct
    68101 ccactaaacg gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg
    68161 aggagctatt ttcccgtttt tttctctaat actcaggtca aaccgctgat ttcacaacct
    68221 ctgattcacg gtgttccaga agtagcttct tcggaaataa gccgaaattt tacaaaaact
    68281 tgagaagcac ttttcgtaaa tttcttctta tttcttgaag ctgaccactt ctgtcacaac
    68341 ttctgttttt cattgtccat aggtttgata ttgtccttct gatgactcat tcgtaatgat
    68401 tgaggcttga ttttcttcat ttcccgttat cgtttgtcca ctgccagaat ctgaagggct
    68461 tagttctgtc gactcgtttt tttgagaaga agcagttgtt ttcttgactt gttcgttggt
    68521 ctttttccat tccgctagtc gatcggccac cacttttttg tccacttttt cagaataagc
    68581 tgtaaccact tttccatttt tcaaacgtgc taccttgtcg ccatcatgag tcaaaagcaa
    68641 aacgaccaaa tgtttgtctt tgaattgata ttgtctgaga gaaatcgcac taagtacgtc
    68701 atcgatattg ataaattgat tttcttcttt tccttttggc aaatagatgt ccaattcacc
    68761 tttttcgtaa ttggcagtta atttatattc ctcccaccaa taagttgaga attctttttt
    68821 gaaattatct actgattctt tgcttgctac gattttttct gtttctttct gagcaagatc
    68881 tgctgtcatt tttcctgaaa atgctgcgga taaaataagc agtaagaaaa aaccgaataa
    68941 ccaaaagaga agaaacggtc tttttttcct catgccttcc tccactccta tttaattttg
    69001 actattctat tatatacaaa aaatgaaata gaaagacaaa aaagttgaaa aagatcgtaa
    69061 ataattgaac ttctttttcc tgaagcggta agaacgggaa ttttcataca ttctctcttg
    69121 gctattcttt aagcttcaaa tccatttctg tctgatacat ctttgaacca atgacccgat
    69181 ttcttgatcg ttctttttcc atcttgtgct aaatctactg aaataaaacc gtaacgattc
    69241 ttataggcat tggtccacga ccagttatcc atgcatgtcc acatatgata acctaaacaa
    69301 ttggcacctt cttgaatggc ttgatggaca tatttcagat gagattgaac aaattcgatc
    69361 cgataatcat cgtggatcat accatcttcc ttgataaatc gtgcttcatt ttctacaccc
    69421 atgccgtttt cagaaataaa acagtcgata ttcccataat gatctcgtac attgatcaaa
    69481 atatcataga ttcctttttc atagatctcc catccgcgat aaggattcat tttacgacca
    69541 ggcatttcat aattgtcgaa gtaatcctca ggcataggac cgtttttagt atcgattggt
    69601 gtttcttttg cttttattcg gcgtggttgg taataattaa ttcccaaaag atctactgtg
    69661 ttttctttaa tgatcgtcaa atcatctgga tgaatctccg gcattagatc gagttctttt
    69721 attctctcaa tcaattcact gggaaactcg ccttttacag ctggatcaag gaatgaacga
    69781 ttgaagaacg catctgctat tcgtgctgct ttaacatctt cgggatttga ttcgtctctt
    69841 ggataactcg gtgtcaaatt taagatgatt ccgatcacac cgctttgatc catttcatgg
    69901 taagctttga tggctaatgc tgaagccaac gcttcatgat aaccaacttg gattgccttt
    69961 ttcatatctg attcttccgg ataatgccat cgatacaaat agcctgcctc gactggaacg
    70021 attggttcat tatgggtaaa ccattttttt actttcgagc caaacaattg aaaacaactt
    70081 tttgcgtatt tttcataggc ttcaaccgtt tctcggttca accatccgcc tttttcttgc
    70141 agatacatcg gcatatcgaa atgatacaga ttaacaaagg gttcgatacc gttttcaatc
    70201 aactcatcaa aatagtgatg atagaaacga acggcttctt cgttgattgt tccagattca
    70261 tcaggtatca aacggctcca ttggatagag gttcgaaatg aattatgccc agtctgtttc
    70321 atcaactgta catcctcttg atacttcgta taaacttgcg aggttttttc aggtcctact
    70381 tgatcaaaga acttttgcgg ttcgtttttg taccaataat caaaaatatt ctctcctttt
    70441 ccatcgcctg aaattcttcc ttctgtttgt ggaccacttg ctgcagagcc ccaccaaaaa
    70501 ttttttggaa attgatatct tttcatcaaa gcacctcttt ttctttttta gttgattcca
    70561 tgtatgatat tttccatcac aatcactttt tcattttctt cctcttcttt tttgattcgt
    70621 ttcaacagct gttctgctgc tcgttccccg attttatctg ttggtggcgt gatcgtgatt
    70681 tttggaagga tactcagatc gtttttatct aaataatcat agtgactgaa cgcaatatcc
    70741 ttcccgattc tgacaccttc ttgattgaaa cattcgatgg caccaatcga catgttgtaa
    70801 ttgctgacaa aaatagcagt tgcccctttt tctaataatg cttttgcccc gtaatagccg
    70861 ctttttctag aatagtctcc tgtaaagatc tctaaatgga tagacggatc cttttgtacc
    70921 gcttctttgg ctccagctaa acgttctctt gctacgtaat ctgtttgagc ggcagcaata
    70981 aatcctattg ttttatgtcc ttgtccgcca tgatgagcaa tcacttttgc tacactttct
    71041 cgatcattca ctactattgt atcaatacct gctggcccac ccggacaatt caatgcgaca
    71101 actgggatac tcatttttgt cagcatttca aatccagacc actcttcttc tgataagaag
    71161 acgatcaatc catcgatcgc atgcttcatc aggtattcga ttttttgttc gattttttct
    71221 ggctgatcgg caaacccgct taataataag ctatacccat tttcttcagc tactctttcg
    71281 atccctgaaa caacctccga accaaatcgg ttagatatcc cgtttatcaa cagtccaata
    71341 gagaaacttc gattattctt caatccttta gcaatgatgt tttctttata gtccaacgct
    71401 gaaatcgctt ctgatatttt ttccatattc ttttccttca gctgctgacc gtttaaatag
    71461 cgggaaactg ttcccactga aacaccagcc atcttagcca cgtcacggat cgatgccata
    71521 cattttcact cctgccagtt acatttgatt tctttttctt gttgttcgac catgatgacc
    71581 gctttctttg cataatgccg cttgtcatca atatccatat aactaaagtc ttcagaagtg
    71641 agttcaaaaa caagcagtct ttcctcttga gaccgcaatt catctttacg aaaagctttc
    71701 aataatttcg ttctttggat gacaacgccg tcaattaatc ggacatagac aagagtactt
    71761 gttttacctg aaacacttcc tgtattttta actttaattg atattattac tttgtcaata
    71821 tccgttttga cttccatctg actgtattca aatgttgtat agcttagacc atgtccaaaa
    71881 ggataaaatg gacttccgct taaatcataa taattctctt gtttagaagc agcacgctga
    71941 tagtaataaa caggtagttg ccgactattt ctaggataag acaaactcaa tcgtccgctt
    72001 gggttatttc ttccagaaat catctgcgca atggcttttc caccctcctg tccaggaaac
    72061 catgctgttg caatggcatt cgtaaatgac tcgattttca tcaaatcgta gggtcttcct
    72121 tgcacaagta tcgtgatgat tggcttgcct agttgataca attgttcaat cagctgttct
    72181 tggtatccac ctaaagaaag agaagcaaca tctacatttt ctccagaatc catgttgatc
    72241 cctttcgatg aaactgcccc gttacgaaga aattccatat caaagttccg cgcacttgat
    72301 ccacctagaa ctgcaatgat cacatctgag ttttgagcaa gggtcaccgc ttctaataat
    72361 ttttcttcct gatcctcctg atttcttact tcacatcctt gtgcataaga aacctttgat
    72421 tggggaaact ctgtttttat ctcttgtact atcttttttt tcaaacggtc ttctgtttgt
    72481 ggtgcagagt agtctcccaa caagtggtaa acatcgtgag catttggacc tataacagca
    72541 atgtttttta cttgttgatc caacggcaat ataccgtcat tttttagtaa agtgatactt
    72601 tcttttgctg tctctaaatt taagtgctct gattcctcca tgagatgatc ccaatactcc
    72661 tttggatcgt ttgcaaatgg ctgttcaaat aaacctaata aaaatttgac agataaaact
    72721 cggcgaactg cttgatctag tagcttttca tctacgactt gattatcaat ggcttctgca
    72781 atcttggtat aggtctcatc ccacaaactg agatcgattc ctgccgctaa tgcatatgct
    72841 gcagcagtct ttttatctgt aaaaacatcg gataaacgat ccaaagccac accgtctgcc
    72901 ataacgattc cttgataacc gatctctttt cttaatgttt gctcaaaaag cgctcgattg
    72961 atatgacaag gtatcccgtc gatgtcatta taagcagcca tgacacctac tgcatttctt
    73021 gtactcttca ataatggtcc gtaaatatcg gagaactctc tgcgtccaat cgttactgtg
    73081 cctgaattat gaccgcctaa agcttctcct tgtgcgatac agtgctttaa cacaacaccg
    73141 atctgatcct tcttcttatt gatttcctct actgtctgat caagaaaaga aactttttct
    73201 gaaataatct ctccttggaa ccctcgaacg accgcttcag aaaaacgagc tgagagaatc
    73261 ggatcttccc caaagcactc ttctgtcctt ccccaacgag gatcttttgc cagatccaaa
    73321 gtcgacacca gcgctaaatg aacaccttta gcagccagtt cttttgccat caatcgactc
    73381 gattgttcta ataatgctac attgaaagta tttccacggc cgatattcgt tggataagaa
    73441 atcccatcca agccttgatg accatgtgga cattcttcta caagcaaaac tggaattttc
    73501 catcgcgaat gctgtagtac atattcttgg actaggttag ccaccttcca gctttctttt
    73561 gcaggaatcc cattcttata atctgtttta gaccacggat cagagcgaaa cagaccgtat
    73621 aaggcaccca gaccgcctcc ccatttaaca tgttctttta acttttctgt taattcgatc
    73681 ttgtctccgt ttttttcata acattcccaa ccatacaaat gctggtttac ctgtcctact
    73741 ttttcagcaa tattcatcct cgaaagtaaa tcttctactc tttggcggat ggttaacttt
    73801 gggttttgat acgggtaatc gtacacctta atatcctcct tctgccaatt ttgccttatt
    73861 agcggctaat acaaatggca ggaataaaaa tacatctaga atgataagta ttactgttaa
    73921 gactgctgct cgtatatcgc ctcctgtagc aataaaagat tgtataaccg gtggagtgat
    73981 ccaaggtgca gcagcaactg ttttactgat gattccaaga cttgtggctg cgtaggcaat
    74041 aaccaagtta atagatggta taatagcgca tggaatcata tatattggat tcatgactac
    74101 tggtaatcca aacataattg gttccgtaat attaaataaa caagtagtca gaccaaattt
    74161 agcgattgat cgctgttctt gacgtttaga agcccacaag atagcaataa ctaacggtaa
    74221 aatacacccg ccacctccga tcaatccaaa tgcattggta aagggctccg taataatatt
    74281 aggaatttct tttcctactg aaaaagcatc catgttttcc tgaatagact gtaacaaagg
    74341 tgcttgccgt ataggagcca aaatactcga accatgcata ccaaacaccc ataagaaatc
    74401 agagaaaaac tgtaaaaata agatgcctgg gacggataaa acaaaagctt tcaatggtgt
    74461 ctgaatcgct aattcaatta attgcggaac attcaaacca gtcgtatggt ttaaagtaaa
    74521 aacgacaact gaaaaaaata aaatgacaac tatttctggt actaaagaat taaatgacgc
    74581 agctactgcc ggtggaactc cttctggcat cctgatttta aaactcttca ttttcgctaa
    74641 tttgataaat aattctgtac tgacaagggc agcaatcatc gccgttgcta agccagacga
    74701 agaagtcaga ctttgtaaga aaacccctgt agcttctgct gtatttccgt ccatcaaagt
    74761 gactgtactt gataaagaca tcaaaataaa cattacggct gtacttagaa ttcctgcatg
    74821 aacaggacta aatgatggat catcaatcac gcctgtttgg atatagtggg ttgccaaatt
    74881 ccagccgata ttgtaacaca cgataatagc taaaatactc atcgtaccat tattcacgat
    74941 tgaagcaagt tcagccaatg gacttagatc aagtattctt tggactgttg catttgaaaa
    75001 aatcagagca ttcagcaaaa ccatgacaga agcagccatt actaacggca tagtaacgat
    75061 aaaactgtct cgaatggcca gcaagtgtcg ctggctactc atcttagata cgattggtag
    75121 cattttttca gatagtctgt ctacgacttt cattatttca tctcctagat tctaaaattg
    75181 aaccggttca tttttaatat agcacttttt ttaaagattg caacacattt ttttaaaacg
    75241 ctttctaaag gtggtggatt ttttatgact tttgaaaaat tattctattt aatggtattt
    75301 tgtgaatttt ttctcttcca tccgtttgaa gttttacttt ccaatggtat aatttcttta
    75361 tcctatcaaa tgggggaagt attttgaaga aattaatcgt actatttatt ttgtctgcgg
    75421 gacttttgac attgactggc tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa
    75481 caaccgattc ttctgtctta aaagcggaaa aagcagatat caccaacagt gtatatacgc
    75541 taaaagtacg taaagatatg gatttatccg cgggacagtt gacatttaca gataaaaaaa
    75601 tggaatggac acgcacgtat cagacacaag gagctgattc ttctgaatca tctgaagaaa
    75661 aaacagtgct gacagatatc aagattacta ccaaagatga cgaatatatt atttcaggaa
    75721 aagaaaataa aaaagaagca acagtcacat ttaccaaaat cggtaattac cgtttgaagg
    75781 ataaggatgg caacatctac agtctatagt gccatctgtt ggatcttctt ttgtttctcc
    75841 ccttcttttc tgctatagta aagaagcaag atgactgtaa aggagacgaa atggatgatc
    75901 gatagtcctg attattgtac atgtaataac aacaaaaata tccatacaga atttaatgat
    75961 caaggaagtt tgagtatatg cgacaattgc cacaaactag tcaaagattc ttttaaatcg
    76021 tttgaatctt atcctgtcaa aaattatgta aaaagcaaaa gaagataagc aaaatcaagc
    76081 aaaactcact atggctgatt tttatgaact tcaaactcag cttcttcctt tgatcaagct
    76141 tcaaaaactg gaatcagtaa aacaaacagt ggatcatttc agttcacaaa tcaaagagta
    76201 acgttttatt tatttcacat ctttgaatat aaaataacca aacgatagaa agacttattc
    76261 tacatcacgt agtaattttt ctttcccgtc gtttggtttt ttacttttag cctaattctt
    76321 gacctttcgc atgctgatta tctgtaaaat attttccagc aggctttcct tccaattcag
    76381 ctaatttttc ttggatctga cgaatcacct tttttgcatc ttctggattt tctacaaaat
    76441 cgaatgtatc cccatccact tgtattttag gacatacatc aaaatcctca taccattgat
    76501 tatagcgggt gttcagtgtt tcgtaatagg tgtataacgt ttcatcgctt tctagctgtt
    76561 catagtcacg cccgcgtttt ttgatacgtt ctagcatcgt gtccaaagaa actttgatat
    76621 ggaccatcaa atcaggacgt ttttttggag caacatcatc taactcgttt agcatcgtat
    76681 ctaataagct atcgtactgc tgtacttcga tatccgttac ccgacctaaa tcagcattta
    76741 gatggaacaa cagactatct tcgtagatgg agcggtctag cacattgtca tcgttcacta
    76801 aagcattttt cattgctaaa aatcgcttat tcaaaaagaa gatttgcaat aaaaatgcgt
    76861 actgctcagg atttgaataa aaaagcggca atacttcgtt atcttcgaca ttttcataaa
    76921 atggtttaga atcaatctct tttgcaagca tatccgtcaa actgctctta cctgcaccaa
    76981 tcgttcctga aatcaaaatc actgacatac aacccttcct cttctataag tactcgccta
    77041 cgtaagtttc aacaggcaat ttttcaaaat catattcacg tacagcttgg tctgatttta
    77101 aatacgtgtg aagactttgt gtaatcatca agttcgtagg taccgtctct tcttgtacga
    77161 tgatcatcgg ttttttcaac atagttgcca cacctaattc gtaagccgtt cctggatcga
    77221 ttgtttgatg ctcaaagtca aggatagcca aaacaaattc tgcatttgtc aactcttcca
    77281 tatctttttc gtatacttcc tgcgcccatc cagcgctgaa tagttcataa ttgctttctt
    77341 ggtgatgacg tggagagtag aaacttgtga ttgttttgtt ttcctccaac gctttttcga
    77401 tacgacttac tcgatcgatt tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac
    77461 tcattatttt gcctccttca ataataactg atttttaata gatactgctc gtaattcatt
    77521 gaccagttcc aatggattgt tggaaaagat cacttgatcc aaccattttt tatccataaa
    77581 atcctctttt gcaaaagttt ctaataattg ttgcaagccg ctgtaataac cgccaatatt
    77641 caatattccg attggcttat caggtgtcag gccgatcgct atccaagaaa gaagttgcga
    77701 aaattcctcc aatgtaccta gacctccagg gaaaacgagg acagcatctc ctttttcaaa
    77761 taataatacc tttctttcat caattgtctc tgtaggaata aatgtagtaa catctttccc
    77821 aggcagctca gtttcaaaaa ggccatttgg atagatacct gtgacttttc cgttattttc
    77881 cagtactgtt ttactaatca ctcccataat cccggagtat gaaccaccgt aaaccagttc
    77941 gatttcttct tgtgccatat attttcctaa cgtttgcgca ataaatttgt aactttcttt
    78001 attgccaaaa cgggaaccgc aaaaaacagt aagtttcata aaaaaacact ccctaactca
    78061 aaagtctatc ttcattctac acgtgttttt ttaaaataaa agactagatt ttgtagtttt
    78121 ttagtggtat atagatcaaa caccactata tatagtattt gcaaaaaaat aatttttcag
    78181 ctattttaca gtaaccaaaa aaataaataa ctagtaaata taaaattgaa acgacttact
    78241 tatagaagaa attgggattt cgtcctgcat gtcaataaca cgcattattt aaatagtcac
    78301 acacttgttt ctcctattaa tgaaaaatcc attttattct cgtgttatgt ttcaccaacg
    78361 cctgatgttt ctctaaataa aaaagccgac gaaccaaata atcgaaattt gatcattttg
    78421 gttcatcagc ttgttcccaa acctttgtat taacgataaa tagctggatt cgtaaaacta
    78481 tcacttggat catcactgct ggaagaaaat tcggtgataa ccgcgcctgt tgcacctgcc
    78541 tggaaccagt gtttaatatt ttgttcgatc gtgtactgat ctccagcgtg aagtatgatt
    78601 tctttttttg ccgtatagta acgttcttgc ccctttggaa taatacagga aagttttttt
    78661 gtttcttttc cttcgacata aagatatact aagccttttc gacaacgaaa ggtttccttt
    78721 tttccttcat tctcatctcg aggtgggtgc cgatgctctg gacaagtttg ataaggcaat
    78781 aataccagtt ctttcccaca gtatcgctca ttattttcat aaacaaataa attcaatcct
    78841 tcagtctcga ttgctcctaa tccaaaatct gtatattcga ttctttctaa ttctgtttct
    78901 ttcaatacta ctcctgaatc taggaacatt tgttgtactc ttttcttgta atccattctg
    78961 ttcctcctaa ctgattgtca agtcatccca agatatctct tcttcctctt tttcttcttg
    79021 gatgacttct gcatttgggt caactggttt tttcaataat aaaatagtta ttcccgtagc
    79081 aaaagaacca gctaacaatg caccaatgaa tgcccatggt ctggtcatcg tagcgactgc
    79141 aaacattccc gatgccggaa tacggctatc tgcgccccaa aacatgctca atccaccgcc
    79201 gactgcccca ccgactcccg ttgctatgac attgcgtgcc aagtcgttca atacgatagg
    79261 gatacatccc tctgtgatct gacagatccc cataggaaaa gcggtcttca atgtatcgat
    79321 ttcttgacga ttgaatatat ttttacgaat gattttccct aagaaataag cgatcgtcaa
    79381 gccgaatggg gttaccatcg aagctaaaat caatacagtg attggttcat agattccttc
    79441 tgaaaatcgt gccacacccc cttatccaga ataaatggta atccagaaac agccgggtat
    79501 ctgcggtaat aagcctaacc aaacgaaaaa tatgagaagc taatatgaga agctattttt
    79561 gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt tttttattta
    79621 tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct ccgaatattc
    79681 aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga tctgtgcgtc
    79741 tgttttcaga atttcttgtt cttcttctgt taaaaatact gccgagaaat catcagaaaa
    79801 tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt tgccaaattc
    79861 ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa tataaggctg
    79921 attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa aggaaaacaa
    79981 aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc ggacttcata
    80041 tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat tgtaaggacg
    80101 attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat ctgatgccag
    80161 cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt ctaattcact
    80221 ttctgtcggt aacatcgaac cgactggata ttttcctgag aaaatatctt ctttaatttt
    80281 atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct ttgctttact
    80341 attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat gtgagaagtg
    80401 tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc aattacttct
    80461 ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc ttcatcgtct
    80521 agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc actgacaaga
    80581 tccatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac tgcattagcc
    80641 atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat cgtgccatcc
    80701 tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg tttctggtaa
    80761 cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg ccagccccat
    80821 tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc tgaagttgct
    80881 gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc gatgtagtcc
    80941 actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt ttccggatcg
    81001 actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg tacttggaca
    81061 tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg attgactgga
    81121 ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg gatatccgaa
    81181 cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt atgccatgct
    81241 tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg acttaaagct
    81301 ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac tttactgctg
    81361 tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt tcgatttttc
    81421 cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat cgtgatcaca
    81481 ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc taatcccgct
    81541 tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat cgaaaaacga
    81601 taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg atcgtaatga
    81661 tcaattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca gtcaaattct
    81721 tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc aacggatagc
    81781 ccttttccgt ctcgatcata agccccttct aattgattgg cagcagttgc tccaccccat
    81841 aaaaattttt ctgaaaaacg tgattgcatc ctgatttcct ccttatttta caattgccag
    81901 aacatcttcg tttgcaataa tctctttttg gtctaattca agtatatcgg caaatgcatc
    81961 agtattcgta atgacaactg gagtgattac gctatagcca gctgctctaa ttgcttcgat
    82021 gtcaaaacga atcaataaat ctcctgcttt gacgagatcc ccttgtttga ctaagatctc
    82081 aaagccattc ccatttaatt ctactgtatc catcccgata tgaatcaaaa tctccgctcc
    82141 gtcagtcgtt gtgatcccta cagcatgtcc agttggaaaa atagtcgtga tctctccgtc
    82201 tgcaggtgca actagttctc ctacttctgg atcaatagcg ataccttttc ccatcacgcc
    82261 agaagaaaaa acttggtctg gtacttcact caatggcagt actttacccg ttaaaggact
    82321 ttttaaggta tgccgtgctg aagtaatcgg ttctcctgca ttcgcatgtt tgttttctaa
    82381 ttgattgtct tgtgtttgtt cgtcaaatcc taaaatcaat gtacctacga atgcaagaac
    82441 aaaagcaatt cctgtagcta ttactgctac tgtcacattt gactctacac catccaccgt
    82501 attaatgaaa gtcggaatcg tcaataagct gaccaaactg ctggagaaag cttttactcc
    82561 ggaaaatccg ataatagctc caccaatacc tccagaaata caagcggcaa tgaatggttt
    82621 cttcaaaggt aatgtcacac catacacagt tggttcagtg attccaaata acgaagtcac
    82681 tgtagaagaa atccctaatg ctcgaagttt cgtatctttt gttcgcaatg ctacagctaa
    82741 agcagcccca ccttgtgcca agatggctgg aagaagcatt ggcatcaata catcaaaccc
    82801 atattgttcg atattcaaaa acatgatcgg tacgaatccc caatgcatgc cgaacatcac
    82861 gaatacttgc cataaacttc ccatgattag acctgctacg attggactaa atccataaat
    82921 cgagttgaag agaccgccta aagcattacc ggcaaccgtc ccgattggtc ctaaagcaag
    82981 aaatgttaat ggaaccatca ctactaaaac gacaagcgga actaaaatca tttgcagaaa
    83041 ttgcggaatc acttttttta cgaatggctc taatttactt tgaacccata cggctagaat
    83101 gattggtatg acagaagaag tataaccaga tggtgaaaga atcactggga tcccaaagaa
    83161 actgatagct tctcccgcac cagcaactgc tgtgatagct ggatacacaa gtgccatagc
    83221 aattgctact gctaaaaact ggttggcttt gaacttttta gctgctgtaa aagcaagcat
    83281 gacaggtaag aaggtaaaga caccatctgc tgcagcaaac aatatttgat aagtaccgct
    83341 atcagctgtc agccagccca tcaccgttgc caatgataag aatcctttca ataccccggc
    83401 ggctgccatc gcccctagaa aaggtgtaaa tatactagag ataatatcga tcagacgatt
    83461 caaaatattt ttggactctt cttttccatc ctccgaaact gcaagacctt cctcatccac
    83521 gatcgattga taaacatctg ctacattact gccaatcact acttgatact gaccgccact
    83581 ttgtacgact tggatcacgt ccgggtcttc ttttaacttt tgcgtatctg ctaaagattc
    83641 atcttttaat ctgaatcgca accgcgtagc acaatgtact aaacttttga cattcttttg
    83701 tcctcctacc gcttcccata cacgacgacc aactgcttga ttatccattg ttttttcctt
    83761 ctttcttttt tatttaaaaa ataaaaaccc agattgaaaa aaacagtcac aaacattcgt
    83821 gaacctattt tttcaatcca ggttttgcct gccatacagt aacaatcctt atgattggat
    83881 gctttttttc tcacttacta ctctttgaat atgaatcgtt aaataaatct gctctgcgat
    83941 ccccatttcc tgtttttgcg tttgtcgaag atactcattg atccgttcga cacaatgaaa
    84001 cgcttttgga tactttcctt gaactaatgc gtagagaaat tcatcttgct cgtcagatcc
    84061 ttcccctttg agataacgct gggcaaaaaa ctgcagatga gtaatgatac gctgataatt
    84121 caaagattct tcatcgaaca cgatgccgaa gaatttactg atgatactca gtatatctct
    84181 taccattgtg gtagcggtca tcgtcacttg catgtcttcg tttccttgtt ggctattcac
    84241 aaaatgaaag gcaataaaac cggcttcatc ctcagaaagt tccacacccg tcttttgttt
    84301 cacgaacaaa agagcctctt tcgctgcttg atattctttc ggataaaatt tttttgtttc
    84361 aaataataat ggattaggca gttggatatt ttcttttgct cgtgtgatcg cataatgaag
    84421 atgatcggtc aatgtcagat aaatattagg cgaaaattca acttttagtt tatcttcagc
    84481 cagttcgact acttgtttga cccattcgat ctcctctgcc ggaatatcag ccatcagctg
    84541 ctggaattgg tttttagcaa aagaatctct taaaacaaat tctttttgga ccgcttctgg
    84601 cgaaatctcg tccccaatct ttttttgaaa agctagtcct tttcccatac aaacgacttc
    84661 ttccccgaat ccatttttcg aaatgacgac attgttattc aaaattttat caattttcat
    84721 tttgcttcgc cctctcaaga aaaataaaaa aacctagaat tcgcctactg tttcttagct
    84781 gaattctggt tttgcctgcc gtacagtaac aatccatttc ttattcaaaa tcatcatacc
    84841 atcgaatttt tatttatgca agccttttca tataaaaaat aaaaaaactg gaacacaagt
    84901 ctatctagaa aacaaccaaa tcagagatgc acctctttgc gtttctcttt ttttcagcaa
    84961 attggttttt ccttatcgat tcatatcctt tggctgtttt taatctgtaa cgacttatgt
    85021 cccagtatcc taaaatgttt ttgtctctag attgaaagca gatctcatat catctgtcgc
    85081 atcttttatc gatgtaacac tttccagttc tccttgtaca actagtcgcg tgacggcgtc
    85141 catgtcgcca caagtaatta gtgtgacaat ttcttttccc tcgacttcat tcagtaattg
    85201 ggtatctgta ggagcaactt tttctttaaa aaatgttttg tatgtataca cattttctaa
    85261 atctgtcaaa tagatcttgg cacccatctc taaattttct agcggtgtaa acagcaattc
    85321 agggttcgtt gcacgatggc ttgctaaggc ataatttcct tttcccattt cttgatctgg
    85381 agataatgtt cctgctccgt aaagtagtga cgtattatcc aagcctttga aaaccggtaa
    85441 attgattgaa actgaaggga ctgcgatact agcaatcact ggtagatctg ttcctctcat
    85501 ttgcgacctc atgacccctt ctgtcgtcat tggttctacc gcatcaaagt caaaactgac
    85561 atctttatcg ttattctttt tcagatcttc ttttgttaca ttcgctatag catatttatc
    85621 tcctgtctct ctgacaaaat aatccttgat ctgttcattg aaaatcaaag ctagtcctac
    85681 taataacaag aggaacaaga ggatattgac tagccatctt tttgcttttc cttttttttt
    85741 cattttctct ccctccagac tcattttact gtttttcttt tgctctttcc aacagccgaa
    85801 tgacacattt ctacaactct gtgtattttt ttcgtacacc tgcgcttttt tctacaggat
    85861 atttcaaatt atttttgatc aatttttctt caatgatctc atctagatcc aggtgaaggt
    85921 tgtctgccat catgtatgaa tagatcaata catctgccag ttcttctttg attcgttcta
    85981 attgtgtttc ttttacttct tccggcgttt tccattgaaa aagttctaac aattcagatg
    86041 cttccaaaga aatagagata gccaaatctt tttcgttatg aaattgtcgc caattacgtt
    86101 catctctaaa ctggttgact ttttccatac tattcatttg cttgccttct tccaatcact
    86161 tcttacttga ttaatcatac cattttttta cataaaaaat agatgatgct tttaaaaaaa
    86221 ctcctgtttt cttttattta aaataaatgt catagaaaat caataattta ggtagccacc
    86281 atgtgtatgc agtagcgtgt cttcttccct tgaggctttt tgtttttcga tacgttcttc
    86341 ttcccaaaca gacaacgcta ctagaatctc tcttaatgat tttccttctt cggtgagcga
    86401 gtactctact ttaggcggga cttggttata ggcttttcga tggatacttt catcttgttc
    86461 taactctctt aactgctggg tcagcatttt ttttgtgatc gtagggattt gcttgcttct
    86521 ccacgcctaa aataagcgat attgctcttc cctttatctg gtgtaagcaa agcagttagc
    86581 attttgacga tcgtgggttc cgcttttcac ttcttatcta cttagtatca tgaagtatac
    86641 tttcgtacta atactaagta gcggaagtgt caactacttt gtttaactac atagcaaaag
    86701 aaacagaagg gtgacaacgg tcatcacgct tctgtttctt ttgaatatgc tgtgttgttg
    86761 tctctatctc cgtgtagact ttcctcaaac ttatcctttg acagtaagtc agtaggctga
    86821 tttgctcttt ctagatgcca gatacatttc tacaaaaaaa tagtgattct gttttgaaaa
    86881 ttcgattttt agttctgcat tttcgtaaac atccaacatt tttttcgcac tgtatagacc
    86941 taacccgcca ttttttttag aggtttttcc tcttttgacc atctccgata gcgtcaaatt
    87001 cgttggattt gccttgttcg ctatactaaa acgtgatccc tctttttctt ccagcagatt
    87061 gatcgtaatc ggactctgat cggctacacg atgatcgaat gcgttagaaa taaaaataga
    87121 taaacagcga ataaaaacaa ccagatcaat cgggattgtt ttcacttcga caggaataac
    87181 tacttggatc gggattttct ccgattctgc tttttctaga aattttaaaa agacacccct
    87241 gacagctggg gatacaatat ttgttagctg ttgtatttgc ggtttttcaa aaatagctgc
    87301 agaatgatct tctaatattt ctaaaaattc caacgtttct tttttatttt cctgaagagt
    87361 aatttttaat gaaaacagta gacttttata atcatgtttg aattcctcta gatgatggta
    87421 atattcttca tttatttgat acagttgaag cgattcctga tgttgtcttt cttttacttg
    87481 gaaactgatc aacataatgg aaaaaaacaa agaaaaaaaa ctgatgacta tcgacaaaag
    87541 agatagaagc agataattag atgagcggaa gtgaacactt cccaatacca agtaaatcac
    87601 gccccagctt aataaaatac aaatacctcc aaaatagtgt tcagttggat aagtaaaaag
    87661 caagttgaga aatctcgtct gtttgtctat actttttact aatagataga aaataaatgc
    87721 gataagtagt gcaaaaaaac ttgctgttcc aagatgtctc tttcctaaat aatccatcag
    87781 atcaaaaacc agaaaaaagc tgagtccagt gatagcaaaa aaaagattac tgatcaaaca
    87841 gctgataaac aggtttttcg tagtaaaata aaaaagaaac atcaataata agccaattag
    87901 gtaaggaaag ctcactttag ggataggaag taaaagtgtc accaccatca aagctataca
    87961 accaaagctg ttttttacta gcagttgttt cgaaaagaat aaatagatac ctaaatctaa
    88021 aatcaaagta aaggtcaaat aaagtgtcac tgttgcgacc atcttattcc tccattgttt
    88081 tgtttcaaaa tgtacacata tttttttaat aggagcctgc atatgaaaaa gtattttatc
    88141 atagaagata accagtttca cagcgaatgg atcatcagag aattggtcaa tcatcatatc
    88201 gagaatatcg tgcagctcac ttctatctct caaatcaatc agttgatcca gttgggcact
    88261 attgataatc acgatgtttt ctttatcgat atcgacttga agcaatcact cacaggtatt
    88321 gatgtcgccg aacaaatacg taaaaaaaat gcacatagta atattgtttt tttcaccgct
    88381 tatacgaatt ttgcagtaga gatcatcaat cggaaaatcc ttccgattgg atatatcaga
    88441 aaagacagga atttatctga acaaattcgt caagtgctag aacagatcaa ttggaaagaa
    88501 cagcagatcg taaatgtcga taaaatcttt attgatcaaa aaggtggaag tctcgtcctg
    88561 tatcctcggg aaattttata tattgaaagt atcaaaggtt tgaaaaacca agttatggtc
    88621 aaaacaacta cagaagataa attagtgaat acttctttaa aacaattgaa gacagagtta
    88681 gaaaagtatg cttacttttt acttttaaaa tcttactgta tcaacttatc gcaattgcag
    88741 aaaattgatt ctgctcatta tgtgttagaa tttttcaacg gtgactcctt actcgttggt
    88801 cgaaagatct ttgaaaagac aaaggaacgt ttccatgact tccaaaaaac tgcttcgagt
    88861 tgatgaataa gagctgagac gtaagcatag cagatttcag ggagtgggcc ttgaatcggc
    88921 tccactccct gaagtcgttg atatgatttt acgcttcctc ttctgtagga gcatctgtta
    88981 acgaccatgt gaatgttgaa acgtagttgt ctacagcata attggataca ggaacaaata
    89041 attccactga ttgtctccct tgttccacat tctctcctaa tgaataaatc catgtgccaa
    89101 tcccttcatt tgctcctgca gttactagat ctactgcctg atctgctggg atttcgattc
    89161 cttcagcgcc agttccagaa acggtagatg gcattccact gctattagtt gcgctatttc
    89221 gaatcacggc gtttttgaat ttcacctttg aaccagttag tttattcgta tcagatgttg
    89281 taccaataaa ttctgttcgt ttgactgata atttccaacc ggcataattt ccgcttttat
    89341 ccgttacttg tacgaaattc tgtacttgtg tttgtgctcc agtactgtcc gttccgcttt
    89401 gtaatgcagc atatactgtt tttgctgtac ctaacttgat tgttccaaaa tcaagatctg
    89461 gagcttggtc aatcgtcagt aatccggatg ttccctgatt tggttcgtca ggtgtaaaag
    89521 gagtatcagg atcatttgga tcaacaggag gaactggaac aacgtcttca ggatttgcag
    89581 catctggttt ggtcacagaa atcgttcctt ctgtagtggc tgagctctta ttctcagcaa
    89641 aaacactttg tgtacataat aatagacttc cagctagaac ggtacttaat gcaattgttt
    89701 ttttcatttc tttttccttc tttcttttaa aataaataat ccagcaaata agccaataag
    89761 aatgactcca acaagtatca gccatcgcca gtcaaatgtt tctggctgat tttttaaatc
    89821 aatatccttt tcattcaatt ggtccgctgc atcatcagtt atttcaaaat cggtatggag
    89881 attccagcgt ttttctttcc aagcaccctt cacaaccgct tgataatgtc ccggagctaa
    89941 tttttttcct tctaatggga caagtaaatc aaaatttgta ttgggtgcta catctatttt
    90001 ttcttttttt attgaaaggt aaggctcttt tgtttctttt ttgtagattt caaccgatgt
    90061 agtcaagccg aacatataag aagcatttgg attttgaaga ttcgccttaa tcacatttct
    90121 tccatcgctt tgatccgcat aagctttttt taatttcaaa tctggggaag actctttttc
    90181 gctatttcaa atcaagattg ctttcacttg agaaaactga ttgacgacac caactgtact
    90241 tttgttagaa acgttctctt ttttttcttg aaacgtgatc cctcctgcga ccacaccagc
    90301 cagctctttt tctggcgctt tcagtgaaag tgtctcagtg accgcacttt gaggaggaac
    90361 agttatttct cccactgttt ttgctaagtt tttcatatca taacttaatg tttcatcaaa
    90421 tttacttttt gattctccat attcgatcac accgtttgaa ttcgtcgtag cggagtggat
    90481 caatgtctca actgttactg atttatctgt tcggttaaag accgtgtagg ggatttcctg
    90541 tgttttccca ggctccatcc ttacatttat atatgttttg ttcttatcaa tctgattagc
    90601 cggtaattca aaatctaccg caaaattgaa ttcggaggca tggacaggtg tctgccaaag
    90661 gatgaatcca aggagaagga tcataagtaa agagcagttg tttcgatgac ttttattcca
    90721 tattcgattc atcaatcatc ctccttgatc tttattctgt cggcgcatct gctaatgtct
    90781 atgttatctc tgctgagtaa ttaccagcta tctgattttc tttggttaag acaagtttaa
    90841 acgccctacc gttatttaaa tattctgaat aatctgcttg cacattcgtt gaattttgtc
    90901 ctctgacaat ttccagttct tcttcattca ctaattgata ctcctctcct ttcttgaaat
    90961 ataggacatc ctgcatagaa aatcctgcat catttgtgaa aggctgagat aattttatcg
    91021 ttaagatcca cggatttttc gtttctcgct gatcataaat ccataatttt tcctgtatat
    91081 ccatttggcc aaatgcttct tgtgtcaaac cagttgcttt gatctgacca aaatcgagtt
    91141 tggaaggggc aagaaatgag agaccacctt ctactacgat cttcgtagca ggtgcaaaag
    91201 gcggggcatc atgataaaca actggcgtat cacttggatt gtgattccca atttcatcgt
    91261 tggtacttgg cttgttctgc acacccttag cagtagcttc tcctgcttgg tcgtttaata
    91321 caatttctaa tacatctcct gcaaaaagtt gattttctgg gatagaaaga acaaattccc
    91381 ctgtatcatc aatagttgtt tctgacaaaa cagcttgatt ttttttcaat gaaaccaagg
    91441 ctcctgcttc acctgtacct gtgatcgtgc gagaagctgc attgatcaat gtggtactta
    91501 aattagcagg ttcaggagga atcacatcga ctgtcgtttt ggcagagtcc agttctgtca
    91561 cttcttggcc acctcttact tttttagcag caacaacttg cacttgatcc tccgttttta
    91621 atctctcatc attgggtact tgaacttcga acaagccgcc ttgttcgcct tctccccatg
    91681 ggttatatcc tgccccttca ttttcatctg tttgtccttg cgtgagagca gtaatttcta
    91741 cagatgattt cgtcgtatct gcgtaagtaa tacgtaaagt cacttctact tcgcctgtcc
    91801 aagcatctct tggctttgcc tcgacacctt cgggaacaac gacatgccca tagatttttt
    91861 tatctgcatt cgttggcact cttaaggatt caatcatcgg gagctgatta ttggcactga
    91921 ttcgattata atttttcatt ccggcacctg tctgagcaaa ttcatttgtt aaacctgcac
    91981 tagggcttga tacgaaattg cctaagtcgg tcccattgat cgataagtct gtcatatcag
    92041 aaaaataatc cgggtctttt tcaaagttat cgaatgttgc gcctgttttt cgccataaag
    92101 cgatttctga atgggcgatc tttaaagaag aatctccatt tccttggaat acccagccgc
    92161 ctatgccatt ggtctcgctt ccaatccgtt tgttttggaa atcaaaatag gtaggagatt
    92221 ctaagtccac attcaacaca tccccactga tgatgccggt cttatcttcc cctgttttcc
    92281 cgaccatgac taaataagtt ttttcacctg ccgtaaaatt taaatcaata tctccagttg
    92341 tatctacagt tgctccggaa tctgcgcgta tatcgatatt tgatttttct ccagtgactg
    92401 taaaatcagc tgaacctgga ctttctagaa aaccagaagc gtcataaaat tggatcgctt
    92461 gatttccttg attgagtctg ttcacgtaag atttgccatc acctgtattt cttaccctca
    92521 atgagcctcc attggttact gtcagctttt gcgtaccatt ctcaaaacga actgcgctgg
    92581 aacgcccact ctgcttatcg atgtcgataa ctgatccggc tccttctacg atcaaagaaa
    92641 ggccttttcc tacaaaacgc atcccattgt tcccgggttg atcgtcatct ccagccattt
    92701 tgaccgagat ttgtccacca cttgatacat ggatttgact agaatctcct tgcaagcgaa
    92761 atccgcccgt agttgctgta ttgatggtga ttttcccacc attcgataca gtgactttcg
    92821 atcctgaacc tctcatttgg atgattccgc gattgttgtc attggtaata ttgatctttg
    92881 tatttaccga aaattcgctt cccgaaccac tgacttcaaa catgacatta ccaggtagcg
    92941 tatcgactgc ctctccttca tcgctaacgc catctgcccg atcataatca agagagacct
    93001 ttgaaccgct taagatatgg attcctggcg ctgtttttcc actaccagtc tcatctcctg
    93061 aatctagaaa accgtagaat ttccctagat acggtccata aagttgacta ccattgatca
    93121 cattcaattg atagcttcta caaaaatagg ctttacccgg agcggcattc agtccttcct
    93181 tcactggttg gacatccaac acaccggaaa caccatctaa tgtgacagtt gcacgaggag
    93241 cgtaaatcag tcctaatttg gacccatcat acaaatcaag tgtcccggta aattttattt
    93301 ctcccgtaaa atgatatctg cttccccaaa aatcggtcat ataagcaatg ataatggcat
    93361 ttccatcgat atttggtgtc aatccacctg gatgtgcagc tgttccttta aaattctgga
    93421 actcgatcga ctgaggtatc tcattgatat aaatatggtg tgtgccgaaa ttgtgagcga
    93481 atccaccaaa atcaataacg atatctcgtt tgatctctaa ttccctgtct aaagtcaaag
    93541 gctgaatcag tcggatctta gttactgatt cgtctgcgag aagctgcgcc agttcttctg
    93601 ttgtggcagc ttcgctgtac ccatccgctg aaacagctct tgccagtgcc gcttcactgc
    93661 tatcagacgt ttgttcagct acttctgagg tctcacttgt cttattttct tcttgccttt
    93721 tattagtaga agcctcactt tgagattctg tagtttcaga agtaatcgat tcttctgaaa
    93781 aagtagtctt gtcaaaactt tgattctctg attgcgttga ttcgtttgtt ttctctacat
    93841 cggcaaaaac ggatgtcacc atcaagagag ggaagacaaa caaaatcgac gcaatcagct
    93901 tttttacaca ttgattcatt ctccaccatc ctctctgacg ttttgacaat tttattttat
    93961 cgttttaatc tatttccaat tgtactttca tatttaaaat atgtattttt atggaaaaat
    94021 ttttctatta ggtgtaaatt tcttttttgt acaaaaaaaa ggctgtgaca aaagtcttgt
    94081 cacagtccct tatcccgaac aagcggtgtt ctcccgccaa ctcctcggtc acaacctcca
    94141 ttttaacaat aaacctgtaa atgtgagggc aatactttga ttgtcacagg taatttatcc
    94201 ccttcatctc catcaacatt cgtcgtcaat tcttcctcat cagctaatga cacaactaat
    94261 tgctcacaag taaggtaggc tagatttttc gtagattcat ctactccttt taataggtct
    94321 ggcaatgttt taagcgtatc gatcaaagaa gaatctttta aataaaccaa atggagctta
    94381 ccatcatcca cttttgcatt tggaagtaat gtttcaaaac cgccgactga gttagttagt
    94441 cctatcagca aggtactgct ctcaatttct tccttctttc catcaacttc tacgtgaaac
    94501 gagtaggatt gtgtggaagc taactgtttg attcctgaca tgaaataagc tagttttcca
    94561 aatttcgtct tcttctccgc atctacatca ttgattgatt cagggataga gccaattgcg
    94621 acgacattca taaaatagtc gtcatttatt tttccaatat ccaatggctt gactgattca
    94681 atcgaaaaat gattgatcgc ttcctcaggt tctagcggta tccctaatgc tcgagccaag
    94741 tcattcaccg tacccaatgg gaaaaatccg aaatttggcc gatgttcttg ttcagcgatt
    94801 ccactgattc cttcgttgac tgttccatct ccgcccatga caaacacact atgataccct
    94861 tccgtagccg cttcgcgagt aaagtttttt gcatcccctg ctttttttgt atgcaagacg
    94921 accacttcat caaacaccga ttccaatttt gctatcgcta attgctcaaa ttcctttgct
    94981 tgttcaccgc ctgaactggg gtttaccact aataaaactt ttttcatcac tttctttccc
    95041 ttctgattga tttctttaat ataacgatag cataaagaaa aaaattaggc tgtgatgaag
    95101 tactgttcat gaaacattca tttatttttt gacaagataa aacatccata ctatgaaact
    95161 gaggaaaatt aaaacaatga tataggaaat aaaagaaaat gataggcatt gtatacctaa
    95221 taaagtaaaa atcttaggtg gcagataaaa acacaaaaat agcaagagaa tgtaatagcg
    95281 tttcttccac tttttgttat cagaccttat agcgatcaga ggaataccaa atgcataact
    95341 gaaaaactct tgatgtactg ttaatatgtc acggccttga ataataaatg cgacaatccc
    95401 cgctataatt agtgtgccta acaccaaaac ggaaaattga tgaatatatt ttatatacaa
    95461 atgatccatt gtacttcctg cctttttatt tgtgagaaaa acaaatccgt atacgtgaat
    95521 aacttctaga aataaaagaa atttgcaaaa ccactccatt tatctacttc tacgtgcatt
    95581 ttttgatatt gttttcttta tttaagatta tacagaaaaa tatctagggt cgctggattc
    95641 taagtaaaaa aaatttttaa aataaaaaga actaaatcaa gtagtaatta aactactgtt
    95701 gatttagttc taataaaaag atttagatga acagttttat tgatttaaat agttcatatt
    95761 ttttatttat accgcttatt tactacttgc tgtactagaa tctgaagtga atgtactcaa
    95821 aacattttca aagtctttgt ctttgatttt tacattagcg tctttcaata cttcgccaat
    95881 tactttattc tggaaggtac tgtcacttaa ttttgtgtct gttgcaattt ctttcaattc
    95941 ttttttgtat ttatccatgt cgttgccttt gttttggttt ttcaccattt taaccacata
    96001 atattccgta gtataagttg aagcattggt agaagtgatc acatcagaaa cttgaccatc
    96061 tttcaactta aaggctgctt ctttgacttc tgccggaacc gttgtagtag tggaatcaaa
    96121 tttcactttt ccgccatctt cttttgtcgt agtatcagtt gacttgtctt tggctaattt
    96181 agagaaatcg tctcccttat cagcagcttt cttcacatct tttgcatcat cttcacttgc
    96241 tacttggata atttgagctt ccacttctgg atggaaagat tcccaagccg tttttaaatc
    96301 ttcatcggtc aatttgatat gtttctttag accttcttga aaggctagac tttgtttgat
    96361 actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc aattgtgaat caaatgaatc
    96421 acccaattgt ttctttgttt gatcatattg agaatcgatt tctttggttg atacgtcatc
    96481 tccatattta tcttcaaata cttgataaac gatcaactgt tgcacagcgg aatctgctcc
    96541 ttgcattttt ttcaagctat ccattacttc ttgttcgctg attgtaccgc ctttataact
    96601 aacggctgtt ttttcaccgg cattcgtaca gcctgacaat aagccagtgc ctagtacgat
    96661 ggcaagtgct aaaaatcttt ttttcattta tcaattctcc tacttttcgt tttttgcgaa
    96721 ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga acacctttac taatctgctc
    96781 gatacgtcct gaatagatat aaggctacac aatccagaaa gttcgctcag cagaataaat
    96841 gtcaagtagc acatcacacc ttaatatact acttcttttg ttaccgtttt tcaagtctat
    96901 cttgcgttcc gtatttaacg taactaattc ttagaacaaa tgttatcgat tttgtttttc
    96961 ttcttccgct gctgggatat tcaaatcaga cacttttatg ttcgattttc cagacatttt
    97021 tttgttgttt ttattatgat atatttttat acaactaaaa atgaatatat taaaactacc
    97081 aaaaataact tttaacgctg tttcattata tgttttttct attttatata aacttttgga
    97141 tacttaatac caaccggttg gattgtacca acatgagaat gtgtcagttc tatttcctgt
    97201 cgcacttgca taggcccata ctccttttgt tgcatttgct gatcgaatag agtttccaac
    97261 tttcgctttt gcaccgtgac gattatagac acgactatag tggttagaat gacctgtttt
    97321 tctcgtacaa gttgtatcac tataataaac ccatcttcca gagctatgat taattgctgt
    97381 tctagaaatt tcccggtcta ctggtaaatt tgacttaata atattacctt tagcatcaaa
    97441 atgaatctct cctggcttta aagatttgta atagatttca ccgtgaactg aatttgcttc
    97501 cacttcagat gacatagcgt ttcctccaac aaaacctatc cctagtgcac aaataataac
    97561 tgcttttttc ataaaacgac atcctttcct ttttgatttt taaagcatga tcttcttgtt
    97621 tcctattttg gtcagatatg gatcatgcgt gactacgata actaacgtac cttgggaagc
    97681 ttgttgcgat aagatttcaa ctactttatc cttatttctt ttatccaatg acccagtcgg
    97741 ttcatcggct aaaatgattt tgctattctt caataagact ctcgcacagg cgattctttg
    97801 cttctcacca ccagataatg tgtagatagg cgtacttagc ttcttgttta tatttacttt
    97861 tttcaatgcc tcatactttg ctgttcgctt atctgaaaca tttaaattcg aaaacttcaa
    97921 tccaatatct aaattaaaat cgattgtttc atattcaatc aacccaaaat cttgaaaaag
    97981 aaaagatatg gtatttcttt ttatcaagcg cttctctctc ccagttaatt gtttcgtctc
    98041 ttgatcgaac aaatagaatt gtcctgaact gaactgatca ttcaatccta atatattcaa
    98101 taacgtactt ttacctgctc cactttctcc aacaacagtc acaagatccc cagcatccgc
    98161 agtgaaacga tccaaatgaa gaatcgtttt gtttcgaatg atttttttcg caccttttat
    98221 cactatcaac ggctccaatt gttaacctcc attcatcagc aataactgac ttttcttttc
    98281 tgtagacaaa ataaaaacaa ctgaaagaat aaaatcaatg aagctaaaga aaaaaatatt
    98341 aaaaagatat ttaggctctc tttctataag aaaaaggaag attacacttg tcgttcccca
    98401 tttgatgagg ttcatcatga agaaatcttt atacgtttca aagagcgaat aaccacttga
    98461 acgaaagata gcgattttcc gacaattgta gacaaaatat atgcataaca tgacatagct
    98521 caacaagaag aaagaaaaga aagtgagtaa aaatatctgt atcaagtaat gaaatgatct
    98581 gcttaacact ttgacagcta tctcttcagt ctgagtatac ggaaatgata actgcaagtt
    98641 atcacgtaac tgactttctt caatcagctc aaataacctt tctttcgaca aagagcctat
    98701 cttgatcttt aacggtggat agatttctcc gctgagaata tttctttcca aatacatact
    98761 gtttttcaat gtcagtattt caacaaccgg ataatgttcg atccagggga tagaaggaat
    98821 aaatgtataa atgggttgat tgttttttat aaatagaaat ttcggttctt tttcactgat
    98881 ttcttgataa tattctgtta tttgcgccag gtcatttttt aaatctgtat gtctatatcg
    98941 ttcagggatc aatataacag ggtgcgtttc tttttcggtt atttggatca accttccaga
    99001 gacatcaatc aaaggaaatt tcttaagata attcagattt atttttatcc ctctgccaaa
    99061 ctgttcattt tcttcattca agtaatctga tacattgact aatatacttc cgtttttatt
    99121 caaagtttca tataattcct gatctgctgc tgatgcaaaa cgattggaat aaataaaatc
    99181 cacgtggttt ttcccgatgg ttactggata aaatacatga tagtcctcac cagcgatttc
    99241 ttttggtggt tcatgggaaa cagctaactg tgaagaagaa aaaacgatag ttgctaattc
    99301 gacattggca acggtcaaaa gaagagaaaa caacaatcca aatgtaaaaa gaatgtattg
    99361 gatatagttg aacgcttctc ttaacgaagc gattttttca actaataaaa gagaaatcaa
    99421 agagatacca tatattccgc tgaacaagaa aacgtacatc tggaacaaat aaagaaattg
    99481 ctccttataa tacataaaaa taaaaccacc aatgattcct gcaattatga atgtaataat
    99541 gattttcttt ttccccaata aacaccacaa tacttgatgt aatgaaaagt cattattcaa
    99601 caataaactg atttctttat tcatcaataa gaaaaaggat gataacaaaa ggaataaaag
    99661 aatcaatcca atagttaaat acactcgata attatatacg tctgtatttg aaataaccga
    99721 atacgaatag ctattttcaa agtcttcaaa aatattagtg gcataatcag tgtgaaatgc
    99781 ttgattgaat cgatcagtga atagtgaaat gacccgttca taggcttctt tttctctcgt
    99841 catcaagaaa aattcacctt caaaatcttt tattttgatc gattcttcaa tggacgttac
    99901 gcgaaaggag gtattagaat agggtacctt ctcgtgagaa gatttgacat ccccttgata
    99961 atacaatgtc atttcaagct ttggctgtat cttgaagttc caagatgtat ctacttcatt
   100021 ggattcattt actgtttttt tcatataaga aagctcagtt tctgatgaga cattttcaaa
   100081 taccatgatt agctgctcat attcttcttg cccaataaaa tgttgtggca atcgaatcgg
   100141 agtataagta tctgtttgat ggaaaaattc atttgtccga tagagtacct gtttatttat
   100201 actaaccaag gcaaaaccac tcatgatacc ggtcactatg attgtgagta aaactgtgaa
   100261 cttttttttg attatcacca ctctccctta cttaaatgat tctttgataa aatcataccg
   100321 tttttcatca aaaaaacgta tctatcaagt gtataggtca tgtagaataa atctatatac
   100381 tgtagaatcg aaaggagaag aagatgaaca ccggagaaaa aatacgaact taccgcagaa
   100441 attgtaagct tacgcaaaaa gaattaggcg aaaaaattgg tgtttccgat aaaacgattt
   100501 ctagttggga aaattctcgt acaatgccag actttgaaat gctgtcactt ttacatcata
   100561 ctttaggtct gccgattgat tttccttcta ttgaatcagc taccgaatca atagaaccaa
   100621 cattgccaga tgaacctctt gttaacaaga aatcttattc atcaaaacaa cccttctcac
   100681 tcaaaaagac actatctttc tttctgctcg tcttttgttt ggtctatctt tttcttatca
   100741 attatcaata caaaaatgaa acgatcgatc gattcaatcc ttttttgaaa ccacaaatcg
   100801 gctataccac actccctgta ggatacgtgg acaattccat cgaacgttgg gtatgtaatg
   100861 atatctttgg ccatggtaca tggctgtctt ttcacgctgg agaggtagaa aaagagaaaa
   100921 accatgctgt aatcaaacac aaaggaaaac acgtcatgaa cgttcgcctg ctcacttggg
   100981 aagccattcc ctcagattac caagaaatcc tcagccaaga ctatgacgac gccttaaaaa
   101041 gaagccctca ggagccagat aggcattaag taggagatga acgtagagat agaaaaaatc
   101101 aatgcacatg atcttatcca aaaaacaggg gtacaaatta attgaaaaag ccttgagatg
   101161 aaagttccta aataaaaaag ttcagaatga taggtatttc tggcttatag tataatattt
   101221 acgccacagc cttccaatga ttctttttgg aatcataatc aaggatggac attcacaaaa
   101281 atattttgct cattcgatcc gaatagatta acgaccaatc tagtaagtaa tatagattgg
   101341 tacgtagtca agctgcacat aatttagatc aattttagtt cttttttata tttctatgat
   101401 ctgctcattt acttttatat gatggcttac agtaacataa ataaatatga taatcgctgg
   101461 tagtttatcc aagagtactc aataaaatac aaaatggatt gttcaagcgc ctatcataat
   101521 gataaaatga aacaaataaa cgatacaagg aggaagagac tttgagtaat ggcagaacaa
   101581 ttttttcatt gtaaaaattg ttcagttgtt aatctatggg atttcggtag catgggcatt
   101641 tttttataga ggaatcacac caacctggcc gttttatctc ttgttagtgg gaatattagt
   101701 aggaaatttt gcccctcttc actatcgtac gaactttttt ccaactgatt ctggcctttt
   101761 tcttaaaaca aagacgcgaa aattagaaaa cattattgaa atgacaatta cctgtataat
   101821 cattttctta gttatattcc taaacgtctt ctaataagaa tctcttaaaa ttagcttgtt
   101881 acagttagtt acctagttta tataacacag ttatcttatc ttccttatca acatcttttg
   101941 ccaactcttt gtcttaaatt cgaaacatat aagcagagct acgaacacca agactgtttc
   102001 ggctctgttt tttgcatcaa attcactctt atatagctga taatctcggc tctttaaaag
   102061 aaattatttt cgcttcattt tattataaaa aggctaggaa ccttgatgtt atcaggttcc
   102121 tagcctttat ttaagtatta ccccacagac ccttccatat agattttcaa attacacact
   102181 agtttaagat aaaacaaaca aggaaaaact taccatttac tcaaaaaatt aactcatata
   102241 agtacattag aatgtaaaat agactggtca aaatctggtc aaaaaagtag atgtgacaca
   102301 agttcgcatt caaagcgatg atccatgatt tcgtaatggt caattatatg gatcaaagcc
   102361 acgcgacaag agcgcaatgg atgtctctac cgattggttg aataaacgaa ataaatagaa
   102421 tgtgaaaaag gatcggacaa atgttccagt ttgctcgatc cttttttatt tgaattcatt
   102481 ttcgctttat caaacatcat ttgattactc agttaaatac ttttgacatg agtaccaatg
   102541 aaacatttat tcattaaaat tacttatttc ttatcgcctt tctagccaat gccaatccgc
   102601 cgattattcc agcactatca cctaattcgg gcgtaacaat gtagctagca attggtggta
   102661 tttcaacata tccatttata agctcaacaa atttatcctg aacttttttc ttcatgtgtt
   102721 cttgtttcat tacaccaccg ccaaaaataa ttctatctgg cgaaagcatc agtgttgtat
   102781 tgtatgcaca ttgggcaagg taaaaggctt caatctgcca aaacgaatcg tcagccaata
   102841 aattctggcc ttttacaccc aatcgttttt caatcgctgg tcctgctgcc atcccctcta
   102901 aacaatcttg gtggaaggga cagtttcctg aaaaattgtc ctttggatgt cgcttgatca
   102961 acatatgccc catttccgga tgactgaacc cttccacgaa agtcccattg aacaaggcac
   103021 cacctccgat acctgttccc acagtgaagt atgcaacacc tgctaaccct ttgccagaac
   103081 cagcaacata ttcaccatag caagccgcat taacatctgt tgtccattca atcggaatat
   103141 tgaaatgttg ctttagcgtt cctacaaaat tgaaattctg ccaagcaagt ttcggtgttg
   103201 atgtgatatg accgtatttc tttgatttac gttgaatatc aattggacca aaacagccga
   103261 ccccgattcc tgccaattgt tccttaaaag gtttaaaaaa atcaatgacc tgcttcattg
   103321 tttcaactgg agtcgttgtg ggaaaactga ctctttccaa aatcgtcaat tcctcagtac
   103381 tgactgcaca aataaattta gtccctccag cttcaattgc cccatatatc atcataggct
   103441 tctcccttcg aatatgtgta aaacacttca ttaacgcggc aagcgttctc gatcagtgaa
   103501 atgacgttta gtatttcctg aagcttctgt tgaataccaa ta
//
LOCUS       DACDIP010000004        95065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDIP010000004 DACDIP010000000
VERSION     DACDIP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 95065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.004"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..2122)
                     /gene="pknB"
                     /locus_tag="IV257_000300"
     CDS             complement(53..2122)
                     /gene="pknB"
                     /locus_tag="IV257_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8813458.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(2119..2859)
                     /locus_tag="IV257_000301"
     CDS             complement(2119..2859)
                     /locus_tag="IV257_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8813459.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(2872..4230)
                     /gene="rsmB"
                     /locus_tag="IV257_000302"
     CDS             complement(2872..4230)
                     /gene="rsmB"
                     /locus_tag="IV257_000302"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8813460.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(4230..5168)
                     /locus_tag="IV257_000303"
     CDS             complement(4230..5168)
                     /locus_tag="IV257_000303"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8813461.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(5161..5652)
                     /gene="def"
                     /locus_tag="IV257_000304"
     CDS             complement(5161..5652)
                     /gene="def"
                     /locus_tag="IV257_000304"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8813462.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(5667..8075)
                     /gene="priA"
                     /locus_tag="IV257_000305"
     CDS             complement(5667..8075)
                     /gene="priA"
                     /locus_tag="IV257_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8813463.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(8328..8633)
                     /locus_tag="IV257_000306"
     CDS             complement(8328..8633)
                     /locus_tag="IV257_000306"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8813464.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(8638..9252)
                     /gene="gmk"
                     /locus_tag="IV257_000307"
     CDS             complement(8638..9252)
                     /gene="gmk"
                     /locus_tag="IV257_000307"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8813465.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(9739..10023)
                     /locus_tag="IV257_000308"
     CDS             complement(9739..10023)
                     /locus_tag="IV257_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813466.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(10041..10931)
                     /locus_tag="IV257_000309"
     CDS             complement(10041..10931)
                     /locus_tag="IV257_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8813467.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(10951..11169)
                     /locus_tag="IV257_000310"
     CDS             complement(10951..11169)
                     /locus_tag="IV257_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813468.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(11139..12839)
                     /locus_tag="IV257_000311"
     CDS             complement(11139..12839)
                     /locus_tag="IV257_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8813469.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            13105..13878
                     /locus_tag="IV257_000312"
     CDS             13105..13878
                     /locus_tag="IV257_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813470.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(13959..14528)
                     /gene="thiT"
                     /locus_tag="IV257_000313"
     CDS             complement(13959..14528)
                     /gene="thiT"
                     /locus_tag="IV257_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8813471.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(14594..14696)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(14702..15673)
                     /locus_tag="IV257_000314"
     CDS             complement(14702..15673)
                     /locus_tag="IV257_000314"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8813472.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(15767..17140)
                     /gene="glmU"
                     /locus_tag="IV257_000315"
     CDS             complement(15767..17140)
                     /gene="glmU"
                     /locus_tag="IV257_000315"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8813473.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(17643..18461)
                     /gene="purR"
                     /locus_tag="IV257_000316"
     CDS             complement(17643..18461)
                     /gene="purR"
                     /locus_tag="IV257_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8813474.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(18628..19452)
                     /locus_tag="IV257_000317"
     CDS             complement(18628..19452)
                     /locus_tag="IV257_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8813475.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(19425..20105)
                     /locus_tag="IV257_000318"
     CDS             complement(19425..20105)
                     /locus_tag="IV257_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8813476.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(20128..21066)
                     /locus_tag="IV257_000319"
     CDS             complement(20128..21066)
                     /locus_tag="IV257_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8813477.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(21311..22162)
                     /locus_tag="IV257_000320"
     CDS             complement(21311..22162)
                     /locus_tag="IV257_000320"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8813478.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(22316..24889)
                     /gene="mprF"
                     /locus_tag="IV257_000321"
     CDS             complement(22316..24889)
                     /gene="mprF"
                     /locus_tag="IV257_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8813479.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            25030..25836
                     /gene="proC"
                     /locus_tag="IV257_000322"
     CDS             25030..25836
                     /gene="proC"
                     /locus_tag="IV257_000322"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8813480.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(25915..27471)
                     /gene="rny"
                     /locus_tag="IV257_000323"
     CDS             complement(25915..27471)
                     /gene="rny"
                     /locus_tag="IV257_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8813481.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(27882..28931)
                     /gene="recA"
                     /locus_tag="IV257_000324"
     CDS             complement(27882..28931)
                     /gene="recA"
                     /locus_tag="IV257_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8813482.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(29040..30281)
                     /locus_tag="IV257_000325"
     CDS             complement(29040..30281)
                     /locus_tag="IV257_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8813483.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(30521..31099)
                     /gene="pgsA"
                     /locus_tag="IV257_000326"
     CDS             complement(30521..31099)
                     /gene="pgsA"
                     /locus_tag="IV257_000326"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8813484.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(31308..32192)
                     /locus_tag="IV257_000327"
     CDS             complement(31308..32192)
                     /locus_tag="IV257_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8813485.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(32970..34256)
                     /locus_tag="IV257_000328"
     CDS             complement(32970..34256)
                     /locus_tag="IV257_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8813486.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(34249..35517)
                     /locus_tag="IV257_000329"
     CDS             complement(34249..35517)
                     /locus_tag="IV257_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8813487.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            35769..36230
                     /gene="mscL"
                     /locus_tag="IV257_000330"
     CDS             35769..36230
                     /gene="mscL"
                     /locus_tag="IV257_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8813488.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(36289..36735)
                     /locus_tag="IV257_000331"
     CDS             complement(36289..36735)
                     /locus_tag="IV257_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8813489.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(36828..37073)
                     /locus_tag="IV257_000332"
     CDS             complement(36828..37073)
                     /locus_tag="IV257_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813490.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(37188..37766)
                     /locus_tag="IV257_000333"
     CDS             complement(37188..37766)
                     /locus_tag="IV257_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8813491.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(37843..38373)
                     /locus_tag="IV257_000334"
     CDS             complement(37843..38373)
                     /locus_tag="IV257_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8813492.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(38377..39237)
                     /gene="rlmB"
                     /locus_tag="IV257_000335"
     CDS             complement(38377..39237)
                     /gene="rlmB"
                     /locus_tag="IV257_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8813493.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(39230..39625)
                     /locus_tag="IV257_000336"
     CDS             complement(39230..39625)
                     /locus_tag="IV257_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8813494.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(39622..41034)
                     /locus_tag="IV257_000337"
     CDS             complement(39622..41034)
                     /locus_tag="IV257_000337"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8813495.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(41031..41567)
                     /gene="cysE"
                     /locus_tag="IV257_000338"
     CDS             complement(41031..41567)
                     /gene="cysE"
                     /locus_tag="IV257_000338"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8813496.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(41889..43349)
                     /locus_tag="IV257_000339"
     CDS             complement(41889..43349)
                     /locus_tag="IV257_000339"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8813497.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(43519..44676)
                     /locus_tag="IV257_000340"
     CDS             complement(43519..44676)
                     /locus_tag="IV257_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8813498.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(44710..46080)
                     /gene="radA"
                     /locus_tag="IV257_000341"
     CDS             complement(44710..46080)
                     /gene="radA"
                     /locus_tag="IV257_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8813499.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(46143..46670)
                     /locus_tag="IV257_000342"
     CDS             complement(46143..46670)
                     /locus_tag="IV257_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8813500.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            46900..47706
                     /gene="proB"
                     /locus_tag="IV257_000343"
     CDS             46900..47706
                     /gene="proB"
                     /locus_tag="IV257_000343"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8813501.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            47703..48950
                     /locus_tag="IV257_000344"
     CDS             47703..48950
                     /locus_tag="IV257_000344"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8813502.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            49026..49370
                     /locus_tag="IV257_000345"
     CDS             49026..49370
                     /locus_tag="IV257_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813503.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(49487..49813)
                     /locus_tag="IV257_000346"
     CDS             complement(49487..49813)
                     /locus_tag="IV257_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813504.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(50186..51387)
                     /locus_tag="IV257_000347"
                     /pseudo
     CDS             complement(50186..51387)
                     /locus_tag="IV257_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            51504..52019
                     /locus_tag="IV257_000348"
     CDS             51504..52019
                     /locus_tag="IV257_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8813505.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(52218..52664)
                     /locus_tag="IV257_000349"
     CDS             complement(52218..52664)
                     /locus_tag="IV257_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8813506.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(52711..54189)
                     /gene="msr"
                     /locus_tag="IV257_000350"
     CDS             complement(52711..54189)
                     /gene="msr"
                     /locus_tag="IV257_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8813507.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(54543..55172)
                     /locus_tag="IV257_000351"
     CDS             complement(54543..55172)
                     /locus_tag="IV257_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8813508.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(55156..55620)
                     /locus_tag="IV257_000352"
     CDS             complement(55156..55620)
                     /locus_tag="IV257_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8813509.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(56107..57375)
                     /locus_tag="IV257_000353"
     CDS             complement(56107..57375)
                     /locus_tag="IV257_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8813510.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(57606..58343)
                     /locus_tag="IV257_000354"
     CDS             complement(57606..58343)
                     /locus_tag="IV257_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8813511.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(58410..58979)
                     /locus_tag="IV257_000355"
     CDS             complement(58410..58979)
                     /locus_tag="IV257_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8813512.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            59107..59979
                     /locus_tag="IV257_000356"
     CDS             59107..59979
                     /locus_tag="IV257_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8813513.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            60321..61193
                     /locus_tag="IV257_000357"
     CDS             60321..61193
                     /locus_tag="IV257_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8813514.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            61190..63148
                     /locus_tag="IV257_000358"
     CDS             61190..63148
                     /locus_tag="IV257_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8813515.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(63399..64250)
                     /locus_tag="IV257_000359"
     CDS             complement(63399..64250)
                     /locus_tag="IV257_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8813516.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            64373..64840
                     /locus_tag="IV257_000360"
     CDS             64373..64840
                     /locus_tag="IV257_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8813517.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(65103..65585)
                     /locus_tag="IV257_000361"
     CDS             complement(65103..65585)
                     /locus_tag="IV257_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8813518.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(65685..66548)
                     /locus_tag="IV257_000362"
     CDS             complement(65685..66548)
                     /locus_tag="IV257_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8813519.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            67146..67994
                     /locus_tag="IV257_000363"
     CDS             67146..67994
                     /locus_tag="IV257_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813520.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(68165..68506)
                     /locus_tag="IV257_000364"
     CDS             complement(68165..68506)
                     /locus_tag="IV257_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP8813521.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            68592..69299
                     /locus_tag="IV257_000365"
     CDS             68592..69299
                     /locus_tag="IV257_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8813522.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            69286..69609
                     /locus_tag="IV257_000366"
     CDS             69286..69609
                     /locus_tag="IV257_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8813523.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(69698..70735)
                     /locus_tag="IV257_000367"
     CDS             complement(69698..70735)
                     /locus_tag="IV257_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8813524.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(71182..72165)
                     /locus_tag="IV257_000368"
     CDS             complement(71182..72165)
                     /locus_tag="IV257_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8813525.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(72581..73351)
                     /locus_tag="IV257_000369"
     CDS             complement(72581..73351)
                     /locus_tag="IV257_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813526.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(73344..74279)
                     /locus_tag="IV257_000370"
     CDS             complement(73344..74279)
                     /locus_tag="IV257_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813527.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(74873..76522)
                     /locus_tag="IV257_000371"
     CDS             complement(74873..76522)
                     /locus_tag="IV257_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8813528.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(76717..77613)
                     /locus_tag="IV257_000372"
     CDS             complement(76717..77613)
                     /locus_tag="IV257_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8813529.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(77606..78286)
                     /locus_tag="IV257_000373"
     CDS             complement(77606..78286)
                     /locus_tag="IV257_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8813530.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(78287..79069)
                     /locus_tag="IV257_000374"
     CDS             complement(78287..79069)
                     /locus_tag="IV257_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8813531.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(79062..79979)
                     /locus_tag="IV257_000375"
     CDS             complement(79062..79979)
                     /locus_tag="IV257_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8813532.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(79982..80266)
                     /locus_tag="IV257_000376"
     CDS             complement(79982..80266)
                     /locus_tag="IV257_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8813533.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            81054..84281
                     /gene="fss3"
                     /locus_tag="IV257_000377"
     CDS             81054..84281
                     /gene="fss3"
                     /locus_tag="IV257_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8813534.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            84393..84707
                     /locus_tag="IV257_000378"
     CDS             84393..84707
                     /locus_tag="IV257_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8813535.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            84720..85094
                     /locus_tag="IV257_000379"
     CDS             84720..85094
                     /locus_tag="IV257_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8813536.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            85104..85448
                     /locus_tag="IV257_000380"
     CDS             85104..85448
                     /locus_tag="IV257_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8813537.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            85517..86533
                     /locus_tag="IV257_000381"
     CDS             85517..86533
                     /locus_tag="IV257_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8813538.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLSALFIGWMGVFYFPELRQFGL
                     SKVSFSTIEIAWSMKDLVCLLGSLALSGSMMLLYIHFFPDHWRSLWNRQKLARMILEN
                     HWYEVKQTQSDGFFKDLNRSRTKETISYFPKVYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISKCVEDFYERMMARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            87127..89001
                     /gene="ltrA"
                     /locus_tag="IV257_000382"
     CDS             87127..89001
                     /gene="ltrA"
                     /locus_tag="IV257_000382"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8813539.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            89127..89483
                     /locus_tag="IV257_000383"
     CDS             89127..89483
                     /locus_tag="IV257_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8813540.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            89528..90670
                     /gene="dcm"
                     /locus_tag="IV257_000384"
     CDS             89528..90670
                     /gene="dcm"
                     /locus_tag="IV257_000384"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8813541.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            90660..90949
                     /locus_tag="IV257_000385"
                     /pseudo
     CDS             90660..90949
                     /locus_tag="IV257_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            91205..92389
                     /locus_tag="IV257_000386"
     CDS             91205..92389
                     /locus_tag="IV257_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8813542.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            92404..92499
                     /locus_tag="IV257_000387"
     CDS             92404..92499
                     /locus_tag="IV257_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8813543.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            92528..92749
                     /locus_tag="IV257_000388"
     CDS             92528..92749
                     /locus_tag="IV257_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813544.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            92762..93265
                     /locus_tag="IV257_000389"
     CDS             92762..93265
                     /locus_tag="IV257_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8813545.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            93392..94429
                     /locus_tag="IV257_000390"
     CDS             93392..94429
                     /locus_tag="IV257_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813546.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            94444..>95065
                     /locus_tag="IV257_000391"
     CDS             94444..>95065
                     /locus_tag="IV257_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP8813547.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKD"
BASE COUNT        27687 a        19692 c        17521 g        30165 t
ORIGIN      
        1 agaatgcaac aaatcaatgt cgcattccct tctgatatat tttattcagc acttattttg
       61 aactttctga ggtgctagct gatgaacttg tggagctgga agattcttct tttgaagagt
      121 tgcttgtgct actggagcta cttgagctgc tagaactgtc tgaagagtct ttttcagaac
      181 tagaagacgc tggatctggt cctttagagt aaattacgtt cacaaccatt cctttggtaa
      241 ttgtcgttcc tgcccctggt actgtacgaa tcactttatc tttatccacc gtgtttgaat
      301 attcggaagt ctcatgcccg atatattctg caccgacact agctaaataa ctttgtgctt
      361 cgctttttgt atagccggag atatcattca atttaggctc tgttccttgg cttacgacaa
      421 aattgatgta agtttcttct gctacaactt cacccgaggc tggatcctga ctgatgatcg
      481 aacctgatgg tacagtgtcg ctatagtcat aagtagcact aatattattg atattgaatc
      541 cttgtgtaac aagccaatta cgaacagttt cgtagttttc tcctacatga ctactcaaag
      601 tcactgtttc tggtcctttg ctgactgtga aagtaatcgt gtctgtttta ggatcgactt
      661 ctgtatcttc tgatggagat tgcgaaatga tcattccgct ttctacctct gaactgaatt
      721 cctcttcttt ttgaatcaag tcagcagaaa accctaattc tttcaaactt gcttcggcat
      781 cttcataact tttaccactg taatcatcta atttgatttt tttcgtgccg ctactaatat
      841 acagagtgac ttcgcgtttt tgcttgactg tcgttcccgc tgccgggtct gtcttcacta
      901 cattaccttc ttcgattttg tcatctgcaa tttcttctgt ttttgttgct acttctaatt
      961 tcgcttttgc gagtgtttct ctagcagaag cttcagaaag atcagaaaca tcaggaacaa
     1021 caacttctcc tcgcccgcca gaagcaaaga atacagctgt tccgattcct aaaacagcaa
     1081 gaatagctaa taggatcagc caccattttt tctgtccttt ctttttagga tcaggcagaa
     1141 cttcttcctc ctctggtaag gtttcgtttt ctggcttgtc ttttggcaga ggcatagatt
     1201 tgaatgaatc aggcattggt gcatcttccg taaggggtgt gataactttt gtttcattat
     1261 ccatcacatg aggctgccat ttttcttcgt ttgcacgagc tggtgataat actgtagaaa
     1321 gatcatcaga catttcctcg gctgacttat aacggtctgc aggttcttta gccgttgccc
     1381 gtaaaatcac gttttccagt gcttgtggca catttggatc aagtgctttt atagatggaa
     1441 gatcatcttg aaaatgtttt aaagcaattg ttacagcaga ttcaccatca aacggtacgc
     1501 tacccgtcaa catctcataa aggatgatcc ctaaagcata aatatctgac tgcttcgttg
     1561 ccatacttcc tctggcctgc tcaggggaca gataatgaac agatcctagc atcgtattcg
     1621 tctgcgtgat cgatgtttca gataaagcga tagcaatccc gaaatccgtg atttttacaa
     1681 caccttcatt atcgatcagt acgttttggg gtttcaaatc tcggtggatg atttgatggt
     1741 tatgagctaa agaaatagca gaaaggatct gctgcataat atttactgct gtttcataag
     1801 gaacaggata atgtgtttgg atatatcgtt tcaagtccat tcctttgacg tattccatca
     1861 ccagatactg catgttgtct tcttcaccaa catcgtaaac actaacaatg tttggatgaa
     1921 cgagttctgt tgctgctaaa gcttctctct gaaatcgtcg aatcgcagtc tggtcatttt
     1981 gaaagtcaaa tcgtaagact ttaactgcta catcacgatc caagattaaa tcgtgcgcca
     2041 aaaatacatt tgccatacca ccgctaccga tatttccagt gatttgataa cggccattta
     2101 attttttgcc caactcgatc atacactgtc ctccttatat tcgataagaa gaacggtgat
     2161 attatccgct ccgcctgctt cattggcacg atcaatcagc tcagttactt tatgagacaa
     2221 tgggccttct tgattgatga tcgttcctat ttcttcttca gacagcatgt tcgtcaagcc
     2281 atctgaacat aacagcagtc gatcttttag ttgccaaaga tgagtagaaa catctacttc
     2341 gactgttcca ggcatgccga cagaacgtgt taaaatattt ttccttggat ggttcactgc
     2401 catttcttcg ctgatttctc ctgatttgac tagttcattg actaaagaat gatcctccgt
     2461 caattgcatg attttatctt cacgaatcaa ataagctctg ctatccccaa cattggcaat
     2521 cgtgaaccag tcatctgaca gagcggccgc tacgattgtt gttcccatac cgttatattc
     2581 cggctgttct tgtccacgct gataaatcag catattttct tcttgaatcg attgaatgaa
     2641 ccattgggcg attttttcat cgtcaacagc ttcttgtttt tcccaagctt cgcctagatt
     2701 cgttaccgcc atctgactgg caatgtctcc ggcacgatga ccacccatcc cgtctgctaa
     2761 tacagcaagc ttgagacctg aacggctggt aaatacactg gcatagtctt gatttgtgtt
     2821 acgtcgtctt cctacatctg attgatattc aatctgcatt ttttcacctc attacttttt
     2881 gcgcaaacaa caaatgaaaa atccatctgt gtaaaactga tgcggataca aagtcaacat
     2941 tttctcttcg attgatgatt gtagcatagg attggtcaaa acatcaatcc tctcaaaatc
     3001 agggtgtctt tccaagaaag cagaaacaac gtcttggttt tcttctttta agatcgtaca
     3061 agtgctataa gtcagaatac cggaagactt taaagttggt gcgcagcttt ctaaaatttc
     3121 caattggatt tttggtaaat tcgctaattc atccggctgt tttttataac gaatatctgg
     3181 ttttcgccga agcagcccaa gacctgaaca aggcgcgtct actaaaatcc gatcaaactg
     3241 ctctgcttcg aactgtttac tgacttctct agcatccatc ttttgagcgt agacagcaga
     3301 agagacacct agacgtgctg cattatcttc aatgagtttg actttgtgtt cgtgtacatc
     3361 taaagcgacc acttctccac cgcatgacgg atctaaaaaa gaagcaatat gcgtcgtctt
     3421 acctccgggt gctgcacagg catctaagac atgatgttca ggctcgattt gcatagacgg
     3481 cgctacaagc atcgaacttt catcttggat cgtcatgacg ccatcgtgga acaatgagct
     3541 agaagctaaa tgcccttttt tcgctactat accgtatgga ctgacttggc ttttttcagc
     3601 tgtgatctct tcttcttgca aaacagtgat ggcctgatct ctggaaagtc ggcgtgtatc
     3661 tactcgggca ctagcatggc taggctgata tagcgataaa ccgagctttt cagtttcttc
     3721 gtatccgatt tgatccacca gtcgtttagt catccataga ggcaggctga ttttgacaga
     3781 aagtctttcg ataggatcag caatgccttg cagatctggt gctccttgac gctgataatt
     3841 cctcaagaca ccatttacaa atttccctgt cccaggattt ccttttactt ttgcgatttc
     3901 gaccgcctcg tttaaaatag catgtgcagg gactttatct aaaaattcta attggtatac
     3961 agaaagataa agaagcgtct tgacccagtc atccactttt tttgctttag cgatcaatgg
     4021 tgcaacgtaa aattccaata gtcgctgatg gctaatcgta ccatagatca attctgtaaa
     4081 taaacccaca tctttttgat ttagctcgct ttgtttgatc atctcattca acaaaagatt
     4141 cgagtaggcg ccacccttgt tcactcgctc taatgtcagt aatgccatat atcgtactga
     4201 ttttttcatt tttttaggga tttttttcat tatttcacct gctgaccaat ctgaacttgc
     4261 tgaccgcttc cgttaaggaa atcatgtacg gcctgttttc ctttaccagc tggctgtaat
     4321 tcattgatgg ctaagacggt ctgtttaccg caagcaatcc atagattttt tttgtctttc
     4381 cggatgatcg taccaggtgc tttgtctgta gtttctgcca atacttcaac cgataataat
     4441 ttccagcgta tgccctcata ggtcgtgaat gcaattggcc aaggacgcat tccgcgtact
     4501 tggttgtcaa tttcttctgc agttttatgc caatcgattg cttcttgttc ccgtgtgatg
     4561 tttggcgaaa aagttgcttc tgactcgtct tgaggtactg gtttttgtcc atccaaaata
     4621 ttgggcaatg tttctagcaa tagctttctt cctaaaaggc taagcttttc aaacatcgtc
     4681 cctacatcat cttgtttagt gattggtatg ctttcttgcg catagattcc gcctgcatcc
     4741 atttttttga tcatttccat gatcgttaca ccagtttctt tctccccatt catgatggca
     4801 taatgaacag gcgcacctcc ccgatattta ggaagtaaag aagcatggac attgattgcc
     4861 cctaattttg gtacttgcaa taacttttca ggcaagaatt ggccaaatgc cgcagtgacg
     4921 atcacatcag gcaccaattc aatgatttct tccatttctg gcgaaccgga gattttctct
     4981 ggctgcagca cacggatacc gtgcttcaga gccgcttctt ttaccggtgt cggcgtaatt
     5041 actcgtttcc gcccaactgg acggtcgggc tgtgttacca ctgcctggat ctcgtaacct
     5101 gattctatca gcccttcaag aatcggcaca gaaaaagcag gtgtgcccat aaatactagc
     5161 ttagtcatca agatgttcct ccatatattc ttcaagttct tcttccggga tctgctgtat
     5221 cattttctca atgaacaaga taccgtctaa gtgatcgatt tcatgctgga aggcacgggc
     5281 taaatagcca aaagccgtca cttctatctc ttctccgtct cgatcataat aacgcacggt
     5341 cacttcatct gctcgcttga ccgttccata gacatgcgga atactgagac aaccttctac
     5401 gtcaaggctt tcaccttttg cttcgatgat ttctgggtta atcagttcaa atttctcgcc
     5461 ttcatctact tcgatcacag caattctctt gttttgtcct acttgaggag cagcgatccc
     5521 tattccgtca ttcgcaatca ttgtctcata taaattgtct aataaagcga ttgtctcgtc
     5581 cgtgattaca tcgattggct gagcagttct ttttaattta tcattagggt ggatcaatat
     5641 aggatagcgc ataatttggc cctttcttaa ataaagttta atggttcgtt gtcaattgcc
     5701 acaaaaagac cttgtcgctg ttctttctgg cttccattca aaatttcatc caaaacatga
     5761 ggaagattcg gttcatgttt atatttgatg attagttgat aataatatcg gtttttcact
     5821 ctcaaaatca tgctgggcgt tggccctaat agcaagcttt gagggcttaa cacttctttt
     5881 agctgattgg caatctgaaa gattttcttt gccgctactt gttcttcagg atggctggcg
     5941 gttattttca cagtaaagta ataaggagga tagcctccac gatgccgtac atgcatttcc
     6001 tgctgataaa actgctcata attttgtttt ttagccaatt ctatggcgta atgttgagga
     6061 ttgaatgttt ggatcacgac ttcgcctgct ttttccgctc gccctgctcg tcctgaaact
     6121 tgggtaagca attggaatgt atgctcacta gaacgaaaat ccggtaaatt cagggaggta
     6181 tcagcattca acaccccaac taatgtcact tctggaaagt ccaaaccttt agcaatcatt
     6241 tgtgtcccta ataatatatc tgcttctttt gcaccgaatt tttgtaaaat ctgttcgtgt
     6301 gccccttttc ttcgagtggt atctacatcc atacgcaaaa tcctagcttg cggataaagt
     6361 tctcggagtt cctcttctac tttttgagta cctgttccat agtagcggat tttattgccg
     6421 ccacaattag gacagcgatt gggaataggt tcttcatgtc cgcaataatg gcagcgcatt
     6481 gaacgtgtat ccatatgcag tgtaagagag atatcgcaat taggacatgg aagaacaaat
     6541 ccacagtccc tacacatcac aaaagacgaa tatcctcgac gattgagcag taggacagtt
     6601 tgttcttttt tgtctaatcg attttgtatt ttttcctgca aattagcaga aaaagtagag
     6661 gtccgatgat tctgaaactc ttcacgcata tctacgatct caatagctgg aagctgtgca
     6721 gctgctttgg ctcggtggtt taactgcaat aactgataaa cgcctttttg agctcgtgct
     6781 cttgattcta aagagggcgt cgcgcttcct agaactattg ggcaatgatg atatttactg
     6841 cgccaaattg ctagatctct cgcatgataa cgcggtgtct catcttgttt gtaacttgat
     6901 tcatgttctt catcgataat gatcagcccg atatttttca gcggggcaaa aatagcagat
     6961 cgtgcgccta ctactacttg cgcttcttcc cgctcaatct tgcgccattc atcatatttt
     7021 tcaccttggg aaagtccgct atgtaagacc gcaactgatt ctccgaagcg tcctttgaat
     7081 cgctcgacca tttgcggagt caatgcgatt tcaggcacta acataatagc cgttttattt
     7141 tctgacaaaa catcagcgat tgcctgaaga tatacttctg ttttcccgct gccagtgatt
     7201 ccctccagca aaaagacctg atctttttgc tgctgacctg ctgtgatgat ttgttcgaca
     7261 gcattttttt gttctgcatt cagttccaat gccgtcgttt ggtcaaatac tcggtcttta
     7321 taagggtcgc gataacgttc tgattctata aactccagcc agcccttttc ttttccttga
     7381 ttcaagacag cagtactgat tcctttattt ttgaaataag caattggttc ttcttggctc
     7441 agttcagata aacacaaaag aagttctttt tgtttcttgg cattttgccg taattttgcc
     7501 cattcttctt cgatttgctt ttcagttaac aaagaacgga ccaaacgaat cgtttttact
     7561 ttatttcgtg tatggacttc atatttgata tctactaatt gctcttggcg ccagcgaatc
     7621 aattgagaaa ggctgccttc ttcttctgct tccttccagg aaatcacttc tcgctgatca
     7681 aacaatacct gaacttctgg tgtgtctgcc aaaggataga tcaatttatc atattctgct
     7741 ttcatcacac tcggaagcat cgtttgtaga caagtgattt tgaatgcgta agtaattttt
     7801 ttcatataat cagctaatga aagtaattct tcattgacca ccggagcaag gtctagcaaa
     7861 cgaataactg ccttcaaaga tggattagct gattcttctg atttttggat agccataaca
     7921 aatccttgga catgacgatt gccattacca aaagggactt ctactcgcat cccgacttgc
     7981 aaagctgttt ccatttcaga gggaaccaaa taagtgaatg gctgatcggt ctgcatagta
     8041 ggtacgtcca caatgacttc agctgttaat gtcatggttc gtttcctcct ttttttgtct
     8101 tttcttatca agcgacaaaa agttccttgc ctattttact agagatcgat ggatttgtct
     8161 tatcttctgc caaatcctaa caaaaaatta acagcagata agaaaaaaaa gaactgtgtc
     8221 aaaaagttca gacacagcct tttctaccag acaataagtg gcgaatgttt gaaaaggaaa
     8281 cagctgggtt tgcttttctt tttagatgtt cgctctattt ttctttatta cattttttgc
     8341 tcttcacgga tacgtgattc caattcgtgt tgttcacgct ctttttgtgc ttgacgttcc
     8401 tcttcttcca tcttcaaacg ttcacgtttt aattcaggat gtggatcatt gactacattt
     8461 cctgcatcga tttcttctaa agcttgaccg acgcttttta ctgaatcaaa agaatctaga
     8521 gtaggctgtg cacttgcatc caattcatgc gcgcgtttac ttgctaaaat cactagagaa
     8581 tattttgaat ttacacgatc taatagtgag tcaattgaag gttttaacat cataagacta
     8641 catctccttt aacattttaa tatatttacc gattacgcga tctactcgaa aatgttcact
     8701 ggcaataatg tttttgattc gttcaactgc tagcggcact tcatcattta caacagcata
     8761 gtcgtaaaga gccatcattt cgatttcttc ttttgcaact ttcattcgtt cttcgattac
     8821 ttcatctgca tctgttcctc ggcctacgat ccgtgatttt aattccgcta aatctggcgg
     8881 agtcaaaaag atgaatacac catcgggcac tttttctttg acttgttttg ctccttggac
     8941 ttcaatctcc aagaaaacat cttttccttc atccaatgtc ttgttgacat atgacaaagg
     9001 agttccataa taattcccga catattctgc gtattccagc atctctccag cttcgatcat
     9061 tgattcaaac tcttctttgc ttcggaagaa ataatcaacg ccttcgacct ccccttcacg
     9121 cattttacgg gtagtcattg agatcgaata ctgaaaatca ttatcctcac tctcaaaaat
     9181 tgcttttcgt actgtgcctt ttcctacacc ggatggacca gacaacacga ttaataatcc
     9241 acgctctgac atgacgacgt cctttcaact agtctttttg tacttatcgt ttattttgca
     9301 ctttttttct gcaactttca aggttatctt taattatagc tagaaattgg taaaaaagct
     9361 accctgtacc aagaggtcgt aaaaaagctg tcctgcatca agtaataaga acagccaaac
     9421 ataaacagct tctcatgttt ttcgtttggt tggggttatt atcgcagatg caagcttttg
     9481 aacgccgtgt ataagaggtc gtgaagaagc cgattagttt cgagtatgaa gaaaaatttt
     9541 gtgaaaatag tttctcacat ttttgcataa atttggctta ataccgagaa aatggttttt
     9601 gaacatcact tattcaaaat aaaaaaagat tgtgacacga attttgtcac aacctttaaa
     9661 tgtattccta gagcaaagaa gctaagcgaa gaaatccaat tctgattatt tattgtattt
     9721 ctttgttcag catccttgct aaaagcccat attggacaaa gtattcacta cacccatgat
     9781 ctcatctttt cgagaacgtg ctttttctgt cggatcttct acctcttctg tgtcaattcg
     9841 aacaataaat ttccgattca tcgtcacgta agcaccgtcg ttgatcaaaa taaacagttc
     9901 gttctcatta tagtctgcac aggcatcttt ccatacgtca aagccgtttt tttcagaagg
     9961 gatatcccgt ttaaccacag tttttccact taattcgtgg aacgttacgt cataatattt
    10021 cattatctct acctctttcg tcattcaata ttttgtactt gttctctgat tttttctaag
    10081 atcgtcttag actggacgac ttgattttta atttcgattg catttgattt ggaaccgatc
    10141 gtgttgattt cccgattcat ttcttggatc aggaaatcta attcccgacc tacaggacat
    10201 tgtgcttcga taagttcatc tagtttttgt acatggatcg ccagacgatc cagttcctcg
    10261 tgaatatctc cgcgttccaa caagatggct agctccgtca acaaccgttg ctcatcgact
    10321 ttcgctccaa gaaattcctc cagctttttt tgatacctta tttgaaattc cttttcatag
    10381 atttctacaa aactatcgag tttttttagt acttctttga attcttcagt attctttttc
    10441 aacactaaag ctaaagcttg accttctttt tctcgactcg cctcaataga atcaagtgcg
    10501 cttttcaatg tatctagagc taactgtgcc aattcttcca tgctctcttc cggtttttct
    10561 tctaccgtta cgaagttttc gtccagcgcg aagcgttccg ccatccgact aattgacact
    10621 tgattttgac taccgtatcg ttgttgaatt ccgtctgtca attgagtgat aagtgaatca
    10681 atcaattccc agttgatgac gatctgtttt tcgtttttgc caatataagc tagattcaca
    10741 aatacttcga ttcgaccgcg gcttaactgc tgtttaataa gctgacgaaa atcgttttca
    10801 acaaagttca ggattttagg acttcgtatt tgaacatcta gaaaacgctg gttgacactt
    10861 tttatttcaa tatctaactg atacgctttt gtttcgcgga tggctttgcc aaaaccagtc
    10921 atacttttca ttcgacaggg tccttcctca ttacatattt tctattgctt tttgcagttg
    10981 ttcaaactct tcgttcgtca atgtcaggcc tttccccatt ttttcatggt ctggcgccca
    11041 atctcgaaga tcgaattttg gcgggcgacc attccagctg actaggttca gttctttacg
    11101 ccaccccttg ttgttttctg acaatatagc gatttcttct acgatttcat atgaaaattc
    11161 ttgtgccatg gttgttcctc cttgagataa tataaatata ggatcagtaa tgtgtcaact
    11221 ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt ttccttgtag cagataatca
    11281 aaataaacag ttttcgtgcg ctcattttgt attttttgct ggtattttcg ataccttcgc
    11341 cattcttcag tacttaattg accaatttgt gccaataaat catccggcat actttttata
    11401 tcttgttggc agatcatttg aatcatcatg tctacttgat agatttcatt gggcagtaaa
    11461 aaatgttttt ttgctaaaag ttctagcatc gccaatccgc atgtctctgg tacgaccctg
    11521 ttcttatata aattacgcag tttttgccaa aattttatgc ttttgaaaaa agataattct
    11581 aactgaattt cttttgttaa gtaagacaaa aatcctcgcc agtatgttct tgcaggttta
    11641 acagaatgaa atgtatacca gttcccatag taatagccta atcgtttttt catttccaga
    11701 tagcctaatt gtaaatttaa ttggttcctt tggctatcaa aaagcagaaa gctgcctagt
    11761 gtccacaatg tttgacattt ccaatggata aaaatgtctg gcatacgaat ttttttcgct
    11821 gggcctggcc cttgcacatc tacgacaaat gcctctgttg ctcctttgtt aatcgcaata
    11881 tcaataggta ttttgttgcg atacccaccg tctgcatatt tattttttcc gatctttcga
    11941 taagccatag cgggataaaa tgaagcagag gcgagtatcc aatctggtat ttcttctgtt
    12001 tttgtctgtt ggatatcagt cactacttcc gctagttctg gcagtttagt agacaccgta
    12061 tataaacgta ttttgggatt atgtttgagt aaggacaaat ccaaattgtc tttgaccatc
    12121 ttttccaatg gagcagaaga aaccccgcct tcaattagtg ctgttttcgt catttggcgt
    12181 ctttctcgat agaagcgttc acgaaggtct tcaactgcag ccatttctgg taacgcaagc
    12241 acttgtcttg tcgacagctt tttccataaa ttcacagctt ttttcatatc tccttgtaaa
    12301 atcaatgcgc cgttcaacgc acctaccgag gtacctgtga taatctcaaa gttgatatca
    12361 tgttccttca gcgcctgcca cacaccaatc tgataagcac cgtgtgctcc tcctccacct
    12421 aaaacaagtg ctgtatgata gaccagcttt ttatttaatt gtgtggttcc ttgatagccg
    12481 tttttatgaa aaatacgcaa ccattcttct gatagaggag attgaaataa aaaacaacag
    12541 ttttctttaa taaaaaaacg agcgcaagct tccatatacc gaaaaaattt tttccattca
    12601 aaagtttcac cttcaatcaa cagattatta aaaataacat ctcctctact tttagcaata
    12661 gtaaaataga cggtatattt tttcgatact gctttaaaag cacattgctt ttttgcagaa
    12721 cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa atggaagggg ttgttttgct
    12781 tgctggatag gcgaatacag cgacattttc tgtgaaaagt aaactttttt tatctccatc
    12841 gcgctctcct ttttcttcaa gtataccagt acgtcataaa aaggtcatga gtctcgtatt
    12901 cgactttgta gattccataa gaaaacaatt cataaaacat cctttaaata aaaaatacaa
    12961 aaatcacaaa aattaattga aaatgagagt gttttcaatg aaaacgtgat tttgtaatgg
    13021 aattttaaaa taaaagaaac aagctttttt aatgcaaatg ttgtaaaata aaagagatta
    13081 agaggaaata agaggaggaa catgatgact gagtcagttt atgtacatat tgataccaca
    13141 agtaatgctg ttttaacaaa agggctgact gctactgact ttgcaaacag tatcgtgcat
    13201 tttcctcaaa acttgctctt gcttgatcca tctgcttctg ctggagaata tgaatcccat
    13261 acagggctga aagtaatccg tggtacagaa aatattcagc gtttcttttc ctcttctcgt
    13321 aacaggcgtg gattcgatga attgaagtgg atcgacttta ctgatttaac gatgctgaaa
    13381 gaattgacac ctttagaaat ttcagaatta ttatattttg gtcatatgaa aacacatctt
    13441 cattcgccgt ttttttataa actacagaat aattttgtat acttcgatct taatgatcag
    13501 ctgaatcgga tttattatcg ctatttagat gaattttata ggatttttgc tagtaaatta
    13561 agttcgatct tgtcagataa gatcaatgta aaaaaatctt ttttcaaaaa agaaacacct
    13621 gttgaaaaaa tcagtgtgga attattgaaa gaaatgcgta atttgatgca ggaagggatc
    13681 attttttctt ttgatcaagt tggtttgatc gatggtaaat atcgtatccc aatctatgtg
    13741 attgaagatt atatttggaa attaaaaggc acccggtatc gagaagagga aatcatcgct
    13801 actttaatct actctgtcac cgatcgacac tggcaattgg tagaagatgc cgatctttct
    13861 tatctgccat tgaagtagat ctatttctta tcaaaaaata aaaaggaaac aagaaactag
    13921 gacatgattc tgtctcaagt ttcttgcttc ttttttattt acagctcctg tcgtttatct
    13981 ggcacaaaaa tcgaaggagt cgtttgttgg atccctaata aaacgatgat cgttacaata
    14041 gctgtactca acccgctggc accattcatt acgaaagaaa agagccaaga attcatcccc
    14101 catagtgcat acgctcccca gaaaaccact cctgcaataa aatgccaaaa atatctggaa
    14161 agtgcgccaa tcaatgcacc aaacacgatg ctttttctaa tttgtttcgt attatcgtct
    14221 acatagcctt tttggacatt tgaagccatg agccctgctg cgcctgcaaa agtataggca
    14281 atcgggtact caatcaacac ttgaacgaca cttaaataat aaacttgtcc caacggaaaa
    14341 tgtaggatgc cccaaatgaa acctgccagc atccctgcct ttgtccctct tcttaacgca
    14401 aaaagagtca atggaatctg ccctaatgaa atagaaaaac tgctgccgat ctggatcgga
    14461 atcaacgaca acaccatcgc taacgctgca acaatcgttc cttctaccca aactctattt
    14521 tttcccataa aaaaacttcc tcctttgaat aaaaaagtca caaaggagga agcagctttc
    14581 gaaaatagtc aaacagctgt ttattcacaa ttcctacgct cgcataaccg atacaggtac
    14641 gaagggttta gaaatagatt caatctcagc ccaattggac tcccctttgt gagtttattt
    14701 attatttatt ttttgtttca aacaatggac taactggttt gttttcatgg atacgtttga
    14761 ttgcatctcc cattagtccg ccaacactta cttcatcgat tttgtcgatt ttacgatctt
    14821 ctggaagata gatcgagtct gtaacaacta gacgctcgat agctgaatct tctattcttt
    14881 gtaaagcagg gccggataat actggatgtg tacatgaagc atacacacta gttgcaccag
    14941 cttctttcaa agcattggca gccaatgtga tcgtacctgc tgtatcgatc atatcatcga
    15001 tcagtacaca agttttgcct ttgacttgtc caatgatatt cattacttct gcaacatttg
    15061 cttttggacg gcgtttgtct atgatcgcaa tcggagattt caaaaattcc gctaatttac
    15121 gtgcccgtgt gacaccacca tggtctggtg aaacaacgac aacatcatcg cctttgattc
    15181 ctttttctaa gaaataattg gcaataagtg gcgcacccat caaatgatcg actggaatat
    15241 caaagaaacc ttggatttgg actgcatgaa ggtccaaagt cagcattctt gttgcaccag
    15301 ctttttgaat catgtttgct actaatttcg ctgtgattgg ttcccgtgca cgtgctttac
    15361 ggtcttgacg cgcatagcca taataaggca tgactacgtt gattgttttt gcacttgctc
    15421 gtttcagtgc gtcaatcatg atcagcaact ccatcaagtt atcattgact gggctgctcg
    15481 ttgattgaat aacatagaca tgcgatccac gaatactttc ttcaatgttt acttgaattt
    15541 ctccatcact aaattgagta acagatgatt ttcctaactc gacgccgact gctgctgcaa
    15601 ttttctctgc taatgggcga ttggaattta acgcaaaaat ttttaatctt gggtcaaagt
    15661 aatgttttga catggggacc tccactttct ttttcacaac tctagaattc tacctaataa
    15721 atagtagtca tattttttaa ataaatcaag tgattttgca aaagtcttag tttagataag
    15781 ggagtttttt tgcatatcct tctttattga cttgtcttgc tctcgcaatt gccagcgcat
    15841 attctggaat attgtctgta attgttgacc cggcagcaat cgaactgttt ttagctacct
    15901 cgacaggtcc gatgatattc gtgctagaac caataaagct gtggtcgccg acagtcgtat
    15961 gatgtttgtt ctttccatca tagttgacga agacaacacc acatccgaca ttgatttctt
    16021 ctcccagcgt agcatcacca acataagtca agtgaccgac tttagttcct ttgccgattt
    16081 gagcattttt cacttccaca aagtttccga tatggacgtt ttcgccaatt tcagcttttg
    16141 gtcgtaaatg tgcataaggt cccacatctg catgtttctt gacatggctg ctttcaatga
    16201 ctgagttttc aatgacgacg tgatcctcaa tcctactgtc tacgattttt gaatgtgacc
    16261 cgatgacaca atcagaacca atgactgtat ttccttggat ttgtacacct gcttcgatca
    16321 atgtgtctgg accgatttct acgcctgcat cgatgtatgt cgttgctgaa tcgacaaagc
    16381 ttacgccatt gaccatatgc atctggttaa ttcgctttct catgatctca tttgctttag
    16441 ctaacgcgat ccgatcattg actcccattg attcttcaaa atcatccgtt tgataagcag
    16501 caactgtatg gccttcttct tttaagattt cgatgatatc tgttagatag tattcacctt
    16561 gagcattgtt tgttccaact ttgcttaacg catcgaacaa agcttcgttg tcaaaacagt
    16621 aagttcctgt attgatttct tgtacaagag cttcttctgg tgttgcatct ttttgttcca
    16681 cgattttttc tacgatccca atatggtcac ggatgattcg tccatatcct gttgggtttt
    16741 ctgcttgtgc cgtcaagatc gttgcacttg cattttttcc ttggtgatat tcaaaaagat
    16801 tgttcagcgt ttcagtagtc aataatggtg tatcgccact gataactaac gttgttcctt
    16861 tttttccttc caagaaagaa gcagcttgca aaacagcatg tcctgttccc aattgttctg
    16921 cttgtaacgc atatttactg cgttctccta gttgtgcttt aacttgttct gctccgtgtc
    16981 caacgatcgt aatcacttca tctggtttcg tttcactgac ccgattaatg atatgttcta
    17041 ccataggttt accagaaacg ggatgtaaaa ctttatataa ttttgacttc atgcgggtac
    17101 ctttccctgc cgccaaaata attgcataac gtgcgtccaa ctgcgtcact cctaaatata
    17161 gtttgctgat ttcagtttat cccagtatac ccattttttc aacaaaacaa gtgattttct
    17221 tttatcccag cgttttctgg ataaaaaaat agatagacca taacgggcct atctatttgg
    17281 taaccggtgc tctcctgcca actcctcggt cacaagtcac actactcata aaacatgaaa
    17341 agctgttttt tgaaatcgtt tcttgtgact tagagttaaa cggtaggctt acaacctctt
    17401 cattaaacgt ttttcaatca gctgaccttc ggtattagct caataaacgg caaaatatga
    17461 ggagcaattt ttccgtttat tgagctaata ctcaggacag gacgctgatt tcacaacctc
    17521 tagataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gaaaaaatgt
    17581 ggaaagccat tttcacaaaa tttttcttac tactcgaaac taagcggctt tttcacaacc
    17641 tcttattctg aaaaataatt tcctggcaca accgtaatat ttttcgtttg agtatctaca
    17701 tctttgacat aaagcagtga cgtataatca tcgatggcgc gatgaccgtt gaattttgat
    17761 tcggcaaata cagtgatgcc aacaagttct gattcgaatt cttcaatcaa gctcttcatt
    17821 ccgttgactg ttccgccgcc tttcatgaaa tcatccacta caaggacacg tgaaccacgt
    17881 ttcaaacttc tcttagacag ttccatcttt tcaattcttt cagaagatcc agacacatag
    17941 ttgacactta ctgttgatcc ttctgttatt tttgagtccc ggcgtacaat aacgaatggc
    18001 gcattcagat aataggacac agcttgggca ataggtacgc cttttgtggc tacagtcatt
    18061 acagcatcga ttttttcacc aaggtattta gaagcaatga ttcttccaac ttgacgcagc
    18121 agttcaggtt ctcctaacaa atcagagaga taaacatatc ctcctggaag cagacggtct
    18181 tgttctgata gacgttctgc tagagattca atatattttt tcgcctcttc atatgaaatt
    18241 tcagggatga acaatacacc acctgcagca cccgggattg tttccaaggt tccataccct
    18301 cgttctttaa atgttttctt gatgattccc aaatcttcac tgatagaaga tttagcagat
    18361 tcataacgtt cggcaaagct tgttaaggaa atcaattcat gcgggtgatt caataaatac
    18421 tgtgtcatgt caactagacg ttcactgcga cgtactttca cctattccaa ctccttttaa
    18481 gaaattttat cgttttacat tatataagtt tttcttcaaa aattcgagca ttttatgaaa
    18541 aaataagaaa atagttcggt atttattaaa ataaaaagaa gcctataaaa aatttttcgt
    18601 ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg caaccaatat
    18661 tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga ttgttgctcc
    18721 tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta gaaatcctac
    18781 acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac cgattgctgc
    18841 gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca tcacagcaat
    18901 agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta gcccatccac
    18961 gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata aaaagaacaa
    19021 tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg taatggtcac
    19081 gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc tcaaattcat
    19141 caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga tttctgaata
    19201 ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa cgactagcat
    19261 ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg ctaaagagac
    19321 atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca cacctagcat
    19381 cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga attcatatga
    19441 aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc cgcatactgt
    19501 ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac atgcgcacta
    19561 tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt tgttggttca
    19621 tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt
    19681 tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc tacagcttct
    19741 aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca gcggcctctt
    19801 ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc attgaaagat
    19861 gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt tttctcagca
    19921 atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac caaggttgac
    19981 ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac atgataagag
    20041 acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat ttcgatataa
    20101 cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag ctttcagatt
    20161 tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag tcaagctttc
    20221 taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct cagctacctt
    20281 cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca caaattcttt
    20341 tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac
    20401 ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag tgacaaatgt
    20461 gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt caagcttttt
    20521 gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt tttcgcctaa
    20581 ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc aaacatgtgg
    20641 atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt ctgcttcttg
    20701 cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt tgctatccac
    20761 tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca cgaaaacatc
    20821 tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg gctccgttcc
    20881 agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg taaaatcata
    20941 catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt
    21001 atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca aaagatattt
    21061 tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat taacgtgaac
    21121 taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg atttaagcat
    21181 gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag ccatttcact
    21241 acaataaaaa actgaagaat accattttag gattcctcag tttttcatca cctattttac
    21301 ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa ccttttaacc
    21361 cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg gttggtccgc
    21421 ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg atttgatgga
    21481 tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt ttgatcatct
    21541 gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga tgttggatgg
    21601 cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt ggtttgacca
    21661 acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct ttgccaccga
    21721 caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc aaaccgattt
    21781 ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct cttagagcag
    21841 ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc ttcttaacaa
    21901 agatcttgac accggttttg atattgtatt tattttttag caataaagca gcttgataaa
    21961 cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg attttgtctt
    22021 ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc accatttcca
    22081 actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt atcttggctg
    22141 gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc gcaagtattc
    22201 tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt ttgtcatcgt
    22261 ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt gattcttata
    22321 attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt cctatcagac
    22381 tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca aaataacgtc
    22441 cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag gaatagaaaa
    22501 aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa aaatctgttt
    22561 ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc attttctcgt
    22621 atacaataaa cagattggct aacagatagt cagctaattc ctcagattct ttaggtaaga
    22681 cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa
    22741 cgactttttg atctttacgg acaataccga caccgctaga taatacatat gatttatcaa
    22801 atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt gaaacttcat
    22861 ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg tatccttctt
    22921 tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct gttgctagat
    22981 caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga agcatcacta
    23041 cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct gcttgtttca
    23101 taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct cctaaaacaa
    23161 agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg tcttcttggt
    23221 aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg cctccgtatg
    23281 tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc gtagtatctt
    23341 cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa ccaataagcc
    23401 ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt attcgaccga
    23461 ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca
    23521 agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga taaaattctt
    23581 ttctggataa ccacacagct aacagaatca agacatatac caatatcaac tgccaagata
    23641 acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta ggcagaaatg
    23701 cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag ccaaccaata
    23761 gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga aatagccgac
    23821 tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc gcaaaataga
    23881 cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta
    23941 aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag
    24001 gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca tcttgccgaa
    24061 cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt ccacctggga
    24121 ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc gaaagcatcg
    24181 aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct cccgtccatt
    24241 gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg aagaacaatg
    24301 tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa
    24361 gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca tcaataaatg
    24421 taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt gatacagtgg
    24481 ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga cttgctccta
    24541 ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca gcaagaagaa
    24601 actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc gtcacccaat
    24661 catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc aggatcgtca
    24721 tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca ttagcaatat
    24781 ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt tttaaaatca
    24841 gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca ttttcccatt
    24901 cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt aatttttcca
    24961 ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga
    25021 gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc tattattgat
    25081 ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag tggacatttt
    25141 gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag taatcaggaa
    25201 gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat tgaaaaagta
    25261 ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc ttcaggaaaa
    25321 tcattaacag atttagaaac agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg
    25381 ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc
    25441 acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag cgtctatcct
    25501 ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc cgcattcgtt
    25561 tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc caaacagaca
    25621 gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct ttcaagtgat
    25681 aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac tgttgcaggt
    25741 gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat caatacgacg
    25801 atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt tttcgtgcct
    25861 tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt tttattattt
    25921 agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc caggataatc
    25981 cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt ctaaatccga
    26041 gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga
    26101 ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac ggatgtagtt
    26161 ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg ctgcagctac
    26221 aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga tcgcgttgat
    26281 tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa cgtgagatcc
    26341 ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac gttttgctaa
    26401 ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa cctcgaccga
    26461 atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga tgatcttgat
    26521 tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg
    26581 aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac gcgctggatg
    26641 gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc ttcggattgg
    26701 atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat cgataccagt
    26761 cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac ctttcatttc
    26821 atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg
    26881 ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt tggcacgctg
    26941 ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag tcgatttcat
    27001 gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt ctttttgttg
    27061 atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct gttttgaact
    27121 aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt catctttacg
    27181 attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt ctaacttact
    27241 ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct tagcttctaa
    27301 caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga ttccagcagc
    27361 tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca taaaccctaa
    27421 accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca taaaatccac
    27481 ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa ttattctaaa
    27541 ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact ttattctaat
    27601 gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta cttattcatt
    27661 aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt taaattgaga
    27721 aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta tgtacccact
    27781 cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag
    27841 tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc
    27901 aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct
    27961 gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta
    28021 gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc accgctttta
    28081 tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa
    28141 ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact
    28201 ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt
    28261 acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga
    28321 ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt
    28381 gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac
    28441 ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact
    28501 gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga
    28561 ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt
    28621 tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt
    28681 ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca
    28741 tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca
    28801 agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata
    28861 gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga
    28921 tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc
    28981 ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat
    29041 cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg
    29101 atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa
    29161 atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg
    29221 gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc
    29281 acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa
    29341 ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca
    29401 gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca
    29461 gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg
    29521 tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct
    29581 tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt
    29641 ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg
    29701 accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca
    29761 gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca
    29821 ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa
    29881 gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga
    29941 ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct
    30001 tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca
    30061 tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga
    30121 gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg
    30181 atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct
    30241 agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata
    30301 tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa
    30361 aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat
    30421 aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag
    30481 agttagacgg caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca
    30541 caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat
    30601 aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca
    30661 gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca
    30721 tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga
    30781 caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag
    30841 tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc
    30901 ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa
    30961 ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat
    31021 ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta
    31081 atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc
    31141 gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat
    31201 tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt
    31261 cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat
    31321 tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg
    31381 gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt
    31441 gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa
    31501 acataggcat tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg
    31561 gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct
    31621 gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac
    31681 tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta
    31741 agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag
    31801 cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc
    31861 caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt
    31921 tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg
    31981 actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg
    32041 taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt
    32101 tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc
    32161 cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt
    32221 ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca
    32281 tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt
    32341 cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt
    32401 aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta
    32461 gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt
    32521 caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
    32581 ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact
    32641 tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc
    32701 ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    32761 tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat tttattttaa
    32821 tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt ttccaacaca
    32881 actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc taaaacgaaa
    32941 tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa ctcgctgaag
    33001 gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg gatcgaatcg
    33061 atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt aaattgattg
    33121 gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt cttcttcagc
    33181 aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat gatttctttg
    33241 actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat
    33301 tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg attgtacatc
    33361 gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt gattgctgtt
    33421 ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt aataccaagt
    33481 acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg
    33541 ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc ttcttggttt
    33601 tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa taaaaccata
    33661 ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt gattttgtcg
    33721 atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata caagttattt
    33781 agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat ttcctgaccg
    33841 atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg
    33901 atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata gctggttttc
    33961 gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg aaagacatca
    34021 ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc tggaactttc
    34081 accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata attcgtactg
    34141 aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt caaaccgttt
    34201 ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt attcatttgc
    34261 atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta cttgttgtat
    34321 ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata attttgtttc
    34381 cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt ctagagaaag
    34441 cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg ttatttcacc
    34501 atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc ggttcttttc
    34561 atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac tagaggcata
    34621 ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc
    34681 aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat acacatttgt
    34741 ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc gttccttcac
    34801 gatattcact tgaggttggg tataaaacag atctggataa actgtttccc gcgactcaaa
    34861 tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga caacaaaaat
    34921 gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac tagcagtgat
    34981 cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa
    35041 ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca aactttccaa
    35101 atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact gatgattttt
    35161 aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg
    35221 atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat gtaaatttcc
    35281 tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt gcgcacttaa
    35341 ctttgtctga tccggataat tcaaactatt tgtttccaac atgctgctga gaagtgaacg
    35401 tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt
    35461 gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat acaccatatt
    35521 tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca acctgccttt
    35581 tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt tttctaaatg
    35641 atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga cggctaaaag
    35701 cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa aagaagggag
    35761 ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg
    35821 ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct
    35881 tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct gatttagaag
    35941 gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg tttggtaacg
    36001 ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta gttgtcaaag
    36061 cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg gaagaaactc
    36121 ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa gtggcagaag
    36181 acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa taaagaatct
    36241 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    36301 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    36361 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    36421 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    36481 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    36541 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    36601 agcttgtcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    36661 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    36721 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    36781 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    36841 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    36901 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    36961 aaaacaccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    37021 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    37081 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca ttataaaaaa
    37141 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    37201 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    37261 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    37321 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    37381 aataaaagat gctgaatcgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    37441 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    37501 gcgttctgct tcctcaacag agaatagatt tgattgctta taatgctttt ataataactt
    37561 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    37621 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    37681 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    37741 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    37801 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    37861 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    37921 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    37981 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    38041 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    38101 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    38161 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    38221 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    38281 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    38341 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    38401 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    38461 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    38521 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    38581 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    38641 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    38701 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    38761 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    38821 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    38881 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    38941 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    39001 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    39061 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    39121 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    39181 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    39241 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    39301 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    39361 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    39421 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    39481 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    39541 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    39601 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    39661 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    39721 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    39781 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    39841 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    39901 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    39961 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    40021 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    40081 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    40141 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    40201 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    40261 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    40321 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    40381 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    40441 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    40501 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    40561 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    40621 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    40681 catatgatcc atgactcttg gatgcagtgt agcaggctcg acattcaacg ctttcgtatc
    40741 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    40801 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    40861 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    40921 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    40981 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    41041 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    41101 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    41161 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    41221 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    41281 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    41341 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    41401 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    41461 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    41521 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    41581 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    41641 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    41701 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    41761 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    41821 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    41881 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    41941 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    42001 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    42061 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    42121 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    42181 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    42241 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    42301 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    42361 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    42421 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    42481 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    42541 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    42601 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    42661 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    42721 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    42781 cgtttttgta tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    42841 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    42901 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    42961 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    43021 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    43081 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    43141 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    43201 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    43261 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    43321 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    43381 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    43441 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    43501 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    43561 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    43621 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    43681 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    43741 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    43801 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    43861 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    43921 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    43981 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    44041 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    44101 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    44161 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    44221 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    44281 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    44341 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    44401 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    44461 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    44521 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    44581 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    44641 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    44701 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    44761 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    44821 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    44881 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    44941 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    45001 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    45061 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    45121 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    45181 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    45241 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    45301 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    45361 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    45421 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cgattttgag
    45481 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    45541 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    45601 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    45661 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    45721 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    45781 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    45841 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    45901 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    45961 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    46021 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    46081 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    46141 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    46201 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    46261 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    46321 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    46381 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    46441 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    46501 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    46561 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    46621 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    46681 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    46741 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    46801 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    46861 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    46921 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    46981 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    47041 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    47101 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    47161 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    47221 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    47281 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    47341 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    47401 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    47461 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    47521 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    47581 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    47641 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    47701 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    47761 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    47821 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    47881 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    47941 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    48001 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca
    48061 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    48121 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    48181 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    48241 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    48301 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    48361 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    48421 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    48481 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    48541 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    48601 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    48661 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    48721 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    48781 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    48841 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    48901 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    48961 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    49021 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    49081 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    49141 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    49201 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    49261 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    49321 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    49381 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    49441 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    49501 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    49561 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    49621 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    49681 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    49741 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    49801 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    49861 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    49921 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    49981 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    50041 cagtctcaca acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca
    50101 accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga
    50161 cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt
    50221 gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc
    50281 tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa
    50341 agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag
    50401 caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga
    50461 tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata
    50521 agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa
    50581 cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta
    50641 attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat
    50701 tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac
    50761 cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg
    50821 ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac
    50881 tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat
    50941 acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag
    51001 tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga
    51061 tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca
    51121 atgttttttc aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt
    51181 gactaacatg taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc
    51241 agcaatcgtt tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt
    51301 agctgctaca actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat
    51361 atctatttga ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta
    51421 ttttacatgt aaatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa
    51481 ataagacgag aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt
    51541 tatccaaacc acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc
    51601 atcacagtca gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt
    51661 tggacaaaga cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc
    51721 ttgtagagga atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag
    51781 caaaaatcta tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta
    51841 gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc
    51901 tagacaagaa acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc
    51961 agcaggaact ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag
    52021 ggataaacag aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag
    52081 caatacagtt ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc
    52141 actatttttc tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt
    52201 caaataacag tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc
    52261 gggccaacga tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc
    52321 atataagcag agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac
    52381 atacatgtca ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg
    52441 ctttctggat ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag
    52501 acatccaaaa tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa
    52561 tgaagatctt gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac
    52621 atcattgttg taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt
    52681 aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat
    52741 tttctgccct tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg
    52801 agaagtaaac aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt
    52861 tgccatatca ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa
    52921 aagcaaagca atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt
    52981 caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa
    53041 ctctgtttgt ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt
    53101 ttgctgatat acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg
    53161 gatatgttct aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt
    53221 acctaaagaa aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg
    53281 gacattttgt cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa
    53341 acggatttgt ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc
    53401 taatgttttc gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga
    53461 caaccgatcc ggtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc
    53521 cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga
    53581 ctctgcttct cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata
    53641 attccccgaa aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc
    53701 atctagaaaa tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc
    53761 ttcaatcagt cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat
    53821 cccttgttca taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct
    53881 ccatttccgc tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt
    53941 taggtccttt gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt
    54001 ttggattttc ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt
    54061 accgacacca ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc
    54121 aatagatagt tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa
    54181 attttccata tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa
    54241 gagcagaaaa atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat
    54301 aactatgact aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa
    54361 aatggacata ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt
    54421 gttattcaaa agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt
    54481 tattaggtta ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc
    54541 ttttattcga acgtagtgat cttttctggt gctaaacgaa cagactcgta accttcttct
    54601 accgcttttc ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat
    54661 gcctctgcca actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca
    54721 cgcgcaacta acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc
    54781 atttcttctc ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg
    54841 acttcttttg gcatcttccc tgattcgtaa gcttggttat agatttcttc gccatattcg
    54901 taacattgca tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta
    54961 ttgaaacgga tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag
    55021 cgccatggtt gcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc
    55081 atttcttcgt gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc
    55141 ataattgttg aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt
    55201 tatattgcaa agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac
    55261 atcgctgaaa gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc
    55321 gattttcttc tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt
    55381 tctctgacgc gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc
    55441 ttgatcaatc tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag
    55501 tctcatcatg atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac
    55561 gattttttga tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat
    55621 aatttcctcc tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa
    55681 aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat
    55741 agagaaaaga aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa
    55801 aaccaagatt attgattctt atcaaactat agaagatagc tcttctgcag tagaaaattt
    55861 tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt
    55921 ggatcagtat gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt
    55981 tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca
    56041 tgaaaatctg tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac
    56101 ctctttttaa taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca
    56161 atctctttcc ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa
    56221 tagtcgataa attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc
    56281 gatttttgtt aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta
    56341 aggattgtat aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc
    56401 atattcccag cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg
    56461 ttctcctgta tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc
    56521 atactgtgtc agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc
    56581 agtggcataa cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt
    56641 ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac
    56701 gttatcttgc aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat
    56761 ccattgccca ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc
    56821 tttaatccca aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact
    56881 ttcaacgatg gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact
    56941 ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc
    57001 ataaaaagca tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt
    57061 caatgtttgc ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg
    57121 agcttctgct gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc
    57181 ttcggttgaa attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt
    57241 ttcaattcct gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc
    57301 agctagcact gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa
    57361 attctttgct ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt
    57421 aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat
    57481 aaatttgctt tttctacata aaaagacgta aatgaagaat attcttctcc attcacgcct
    57541 taaattgatg gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt
    57601 atggattata cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct
    57661 ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct
    57721 ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca
    57781 gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga
    57841 cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca
    57901 ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt
    57961 tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc
    58021 atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca
    58081 ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg
    58141 acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat
    58201 tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt
    58261 tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc
    58321 actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat
    58381 actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt
    58441 ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc
    58501 gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg
    58561 atccaagcat ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga
    58621 tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct
    58681 aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct
    58741 atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct
    58801 actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca
    58861 gtattcagcg ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag
    58921 agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt
    58981 ttgctccctc cttttatcct tactttataa cggacggctt atcatgtcta atacttataa
    59041 aacatactta actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg
    59101 gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc
    59161 agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat
    59221 ttagaagaag aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg
    59281 gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg
    59341 gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa
    59401 actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat
    59461 atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga
    59521 ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga
    59581 ctgccagccc aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa
    59641 accgtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca
    59701 gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga
    59761 acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta
    59821 gctctagatg ggatcatcaa tacttatggg acggaaaaaa tattcatccc actcgatccg
    59881 ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct
    59941 cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct
    60001 ttcagctttt taacaacgaa taagataagc aaaagatcca acgattaaat aacataagtt
    60061 tcttaattta tttcttcctt ttatttctga taattccagt aaattactat aattataaaa
    60121 aaagaattga caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt
    60181 catgtgacta gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg
    60241 tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa
    60301 tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg
    60361 tagatgacgg tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa
    60421 gaaatgattt gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc
    60481 aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa
    60541 cgatcattga tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg
    60601 ctttgaccga ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca
    60661 acaaactttt aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg
    60721 cagtagagta tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata
    60781 tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg
    60841 aagtgacgat tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc
    60901 attcagaacc attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac
    60961 aacgaaccca tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac
    61021 gaaaataccc agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat
    61081 accagctcgc gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa
    61141 gaagcgcaat cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata
    61201 tctacaacga atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt
    61261 actagtaggg atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt
    61321 acaagctggc ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc
    61381 actagggatg tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga
    61441 agttccagta aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga
    61501 atcagttgat ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt
    61561 gattgcggca gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt
    61621 ctttagcggc aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc
    61681 agtgctactg ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat
    61741 tttgagttta ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc
    61801 tacaggattg catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga
    61861 tattggtaaa tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg
    61921 atttttccca atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc
    61981 acgtccagaa aacaaagcaa aaacagcttc cttgatgcta gcagcagctt ttgcttcatt
    62041 ctttactggt gtgacagaac cacttgaatt ttcatttatg ttcgttgctt ggccattata
    62101 tgtcttgcat gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac
    62161 agcaggattt gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat
    62221 tgcgaatcag ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt
    62281 tggctttaac tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga
    62341 tctttcagat gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag
    62401 caaaatctac gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac
    62461 acgccttcgt ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac
    62521 tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga
    62581 agtacaattc gttgcagatg agatggcaaa actacatggt ggtgctgcag cacgacctgc
    62641 acaaacaaac accgtggtta aaacagaaac atttgccggt gaaacgatcg aacaagatat
    62701 ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc
    62761 ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc
    62821 aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga
    62881 tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc
    62941 attcacttta tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt
    63001 tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa
    63061 tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa
    63121 aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata
    63181 aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg
    63241 ttctcctgcc aactccgcgg taataagtcc agctaaacga aacatatgaa gagctatttt
    63301 ttatttgact cttcttatta cttgatgcag aacagctttt tcacaaaatt tttcttcata
    63361 ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct
    63421 tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag
    63481 gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct
    63541 tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata
    63601 atttctaact ctttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt
    63661 agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca
    63721 gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg
    63781 aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta
    63841 ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga
    63901 tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa
    63961 gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct
    64021 ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct
    64081 aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt
    64141 tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc
    64201 agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact
    64261 ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca
    64321 taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta
    64381 ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac
    64441 ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg
    64501 taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca
    64561 gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga
    64621 aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc
    64681 gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga
    64741 aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa
    64801 aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca
    64861 tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt
    64921 catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata
    64981 aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc
    65041 tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat
    65101 tatcatggtt tattgactac cggtatccag atttctccca tatatttccc gttggtaact
    65161 cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa
    65221 acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa
    65281 gcgcttacaa caagatacgt tccttttggg aatgggatga ttcgcgtagt gtttggaaca
    65341 tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact
    65401 gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata
    65461 actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc
    65521 ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt
    65581 gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat
    65641 gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc
    65701 actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag
    65761 atctcgggag cttcgactaa cccataatct gaagaaggaa gccattctgc atagattttc
    65821 ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg
    65881 tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag
    65941 ccaatcatgt gcatcaaact gcccttttct tccaagcggt ctccatcaaa tccataagaa
    66001 acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc
    66061 tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga
    66121 acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc
    66181 gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg
    66241 tatctactga tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct
    66301 tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc atctagaaga
    66361 tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca
    66421 ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt
    66481 ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct
    66541 ttaagcatat ttatcttcct ccttgattgc ctttttatca tattatcaaa gaaagtcaat
    66601 tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga
    66661 aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac
    66721 ctttagaaaa ataaattttg gggaagacag attctaagta aacaaagcgg taaaaatatc
    66781 ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac
    66841 tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt
    66901 ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa
    66961 aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag
    67021 aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag
    67081 ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca tgaaaggaga
    67141 actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc ggttacagct
    67201 atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg tccgcaacag
    67261 tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc aacatgactt
    67321 gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct gtcttattcg
    67381 cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc tcgtcccttg
    67441 tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt atagaacatc
    67501 cgcactttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat tattcagata
    67561 aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa
    67621 cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa tcgcttattc
    67681 agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct actctcgaaa
    67741 cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac tgttattatt
    67801 tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt
    67861 tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactct ataaaaatcc
    67921 tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaac
    67981 taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg ataacaacca
    68041 tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca tgcaaaggat
    68101 gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag gtacgcgttt
    68161 tgttttataa gtcatgacaa atttccttcg ttgctggata gattttccgg cattgttgat
    68221 agattttatg atacttttct acattttgta agagaggttc cgctgcatct gtatattgga
    68281 taaaattctg acaacaactt tcaggtgtgt cgaaccaccc taaccctagt gcagcaatca
    68341 ttgttgcgcc taatgcaggt ccttcctcca cacttaaaca atagaccgtt gcatcgaaaa
    68401 tatcagcttg gatttgtagc catgcaagat ttttcgcgcc tcctcccacc gaaacaatac
    68461 gtgtaaatct gcgacccgct agttctttac gtttcaatcc caccattgac aaatgctcat
    68521 taaaaattta gaatattcac aagtttaacg acaattatga aaatttatgt cgtgctaaag
    68581 gagttttgtt attggatgaa aagttagata taaaaactgc gatcaaagac acgttaccta
    68641 ccgttttcgg ttatatcggt attggacttg catttggtat cgttgggaaa gctgccggat
    68701 ttcatccact agtcgtcacg ttgatgtccc tgctggtcta tgctggttct gcccaattta
    68761 tcacagtcag catgcttgct agtcacagcc cattgctttc catcgttttc tcgacctttc
    68821 tagtcaattc ccgaatgatt ctgatgagta tgacgattgc tccttatttc aagaaaaatc
    68881 ggctgcttca aaatctgctg attggcacat tgctgacaga tgaaagtttc gcactgggaa
    68941 tgaataaatt gaactatact ggacaaaagt taaactttcg ttggatgaat acagctaatt
    69001 ggatttctta tttgacgtgg gtgggttctt ctcttgttgg cgcactttta gggaatttta
    69061 ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt atcgggttac
    69121 tttatcttca agtcatttct gatagaaaca cctctaaacg attacagttg atcctgattg
    69181 gtctcacttt gattctggta tatgtcggac tgatttttat cccaagcaac ttagtgattg
    69241 tagttgtaac actgattggg tgcggattgg gggtgtggat caaacatgcc ttcttttgag
    69301 tttacattgc tgactatttt aggttgtact ttagcaactt ggctttctag ggtcctacca
    69361 tttgtcttac tgaaaaaatt cgatttaccg caaccgttgc ttgagtattt aagttttgtc
    69421 ccgatcgtca ttatgtctgc tttatggttc agcagtctat ttacccaaaa tatcggacat
    69481 ctgccgcaga tcaatctcga aaatgctttg gcttcagttc ctacactttt agctgctatt
    69541 ctttcaaaaa gcttattggt gatcgtcttg gcaggaattc tttccctttc gttgattcgc
    69601 ttattataaa taaaaaaaca agagcttatg gcaaattact gtcatgcgct cttgttttta
    69661 tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta tccgttgaac ggaccactaa
    69721 caacgacttt tcctaacata tgtcctgttt ctacgtcttt ggttgcttgt ttcaagttct
    69781 tcgcattcat cccatctgaa tagaccttgc tcaatgtact gcggataatc ccttcatttg
    69841 ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc gatttcatag ttatagtctg
    69901 ttttagcgaa catatactcc cagtcgaagc tgatagattg gtttttccaa acacctatat
    69961 ctaacggctc tttgatccct acgatcgttc ccacatgtcc aaatggtcgg acaaggtgct
    70021 tgatttgatc aaagtagcgg gtaatatcga ataatacagc gacatactct acttgatcga
    70081 ttcctaattc acgaaaactt ttacttaagt cgtgatgata atcgattgga tagtctacac
    70141 ctgttttttt cagccattca aaattcttcg gacttgcagt cgcgtagact tctaggccag
    70201 cccaatgcgc tagttgattc aaaatcgaac cgacgccccc ggatccgttg atcactaaga
    70261 tttttttccc gcgattggca ttttcttctg gagttagacc aaatttttca aataacaatt
    70321 cataagcggt caatgaggtc aacggcaagg ctgcagcttc ttcatctgaa agattttttg
    70381 gtgcaagagc gacaatacgt tcgtcaacta gttggtattc ttgattgctt cctgctcttg
    70441 ttgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact tcgtcaccta
    70501 cagcaacaac tttcccgact ccatcaaaac ctaagatacg taaagcattt cttatcccgt
    70561 tatcttggcg aagtttcgta tcgacaggat taacagatac tgcgttgact tttaccagaa
    70621 gatcccgtga actgggtaca ggtatctgtt ctttttcatc aataaagctt tttggatcat
    70681 cgatcggcaa cccttcataa aaaccgacta ccttcattaa ttccttttct gacatacgaa
    70741 ttcctcctta gcttcatcaa gttggagcat ccatacagtg ttcatttcaa tatatatagt
    70801 atgctatttg ttccaccttt tttattcatc ataattattc tcttacttaa agtaaactat
    70861 ttgcactttt ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttataaa
    70921 cggtgttctc ctaccaactc ttcggtcaca agtcaaaata ttcatcaaac atgagaagct
    70981 gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctgatat
    71041 aaacggtgtt caatcaactg accctcggta ttagctcaaa aaacgacaaa atatgaagag
    71101 ctatttttgt ttggctgttc ttattacttg atacaggaca gctttttcac gacctctaat
    71161 tcacggtgtt caagcaagaa atcattgttc actgaaaagc gttggatgtt ctttttcaaa
    71221 ggtctctaag atctctcgta cttcatcagt agatttcgta ttcatcaact gaactcgcaa
    71281 gtcatttgct cctggaaatc ctttgatata gattttaaag aaacgatgta gtcctgtgat
    71341 ggaacgcggg atgatttctg aataatgatc ttgcagatct agttgcagac gaagtagtcc
    71401 gatcagttct ttttctgtat gttccctcgg gtctttttca aatgcataag gattcttgaa
    71461 aatccctcgg ccaatcatga tgccatcaac gccatatttc tcggccaatt cttctcctgt
    71521 ctggcgatcg gggatatctc cattgatcgt gattagtgtt tgtggagcga tttggtcacg
    71581 taaagcaatg attttaggaa tgacttccca atgtgcatct actttactca tctctttacg
    71641 cgtacgcaaa tggacagaaa gattggctat atcttgatga agtaggtgac tgatccattc
    71701 ttccatctca gacatttccg tatagccgat acgcgtcttg acgctgacag gcaaaccgcc
    71761 agctttagct gcttcaatca attgtgccgc tacttctgga cgaaggatca agccactgcc
    71821 ttttccacgt tctgctacat tagggactgg gcagcccatg ttgatatcga ttccttggaa
    71881 gcccatttct gccatagcaa tgctcatttc tctaaaaaat tctggcttgt ctccccaaat
    71941 atgggcaacc atcggctgtt catcttctgt aaatgttaat cttcctcgta cactgtcttt
    72001 tccatctgga tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga catccggtgc
    72061 tgctgcatgc tttacgacat ggcgaaatac aacatctgtt acatcttcca ttggtgctaa
    72121 aacaaaaaaa ggctttggca gctctttcca aaaattagta gtcatttatt tgttactctc
    72181 cattctatca agcagtgaag tcgcaatctt tctcaaaaac attgtcatta tactaggaaa
    72241 caaaaaaaga agcaaagtga acagcttaaa agatgtttct gattgagcaa aaaaaagact
    72301 gtgacaaaaa tcttatcaca gtccattttc cggaataaat ggtgttcaaa agcttgcatt
    72361 tgcggtaata agtccaacca aacgaaaaat aggaggagct atttttgttt ggctgttctt
    72421 attacttgat gcagaacagc tttttcacaa cctctccact aaacggtgtt caatcagctg
    72481 accctcggta ttcgctcaaa aaacaacaaa atatgaggaa ctattttccc gtttgtttct
    72541 ctaatactca ggtcaaaacg ctgatttcac aacctctttt ctaacttctc aatcctcttc
    72601 ctttattgat caaataccaa gtaacggcgt acaaaacaac gatgaataac acaagtacga
    72661 tcatcgatgt tgctactggc acatcgatcg ttccaaggaa tccgtaacgg aaaccggaga
    72721 tcatataaac gatcggattg acttttgaaa cagcttgcca aaagggcggt aacatagaga
    72781 tggcatagaa aacgccacct agataagtta acggctgcaa gacaaatgta ggcacgatcg
    72841 atacatcatc ataagactga gcaaaaatcc cgttgagcag tcctgctaat gaaaacaaga
    72901 ttgccgtcat caataatgta atgatgacca tgaaccatga atacacatga agtggtacaa
    72961 agaacaagga aatgatcgtt acgagagagc cgacgagaat actgcgaccc acaccaccaa
    73021 tcacaaagcc ccaaataatc acatgcgtag gaacaggtgc taccaagatt tcttcgatat
    73081 ttttctgaaa tttttgagag aagaaggacg aagaaacatt ggcatacgaa ctagtgatag
    73141 cagacatcat aatcaaacca ggaacgatga attccatata tgaaaaacca cccatgtcac
    73201 cgatccgccc accgatcatt tttccgaaaa taacaaaata caaggatgtc gtgatcactg
    73261 gcggaaccag tgtttggacc catatacgca aatagcggtt cgtttccttg atagctaaac
    73321 tttttaatgc agtaaaataa agattaaaca tttttctcct ccgtatgatt attttcttct
    73381 gtgattttca agaacagttc ttctaaacga ttcgacttat tcctcataga aagaactttg
    73441 attccttgtc ttgtaagctg atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg
    73501 acagcgattg tctgctcatc ttcaaatgta caagcatatc cttcaatcac aggtttggta
    73561 tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca gttttgacag aagctctttc
    73621 atacttgtat tctcgatcag ttcacccgat tggatgattc cgatgttgcg acaaagcatt
    73681 tcagcttctt ctagataatg cgtcgtcaaa atgatcgttg taccattttc gttcaattct
    73741 cgtagaaact cccacatctc gcgtctcaac tcgatgtcaa ctcctgctgt tggttcatcc
    73801 aagatcagca aacgtggttc atgcatcaat gccctagcaa tcatcagtct tcgcttcatt
    73861 cccccggaaa gcatacgtgc acggacattt ctcttttccc aaagattgga ctgcttcaaa
    73921 tatttttcgc tgcgtttcat cgcttctttg cgagagacac cgtagtagcc tgcttgattc
    73981 acgacgattt gctggaccgt ttcaaaaggg ttaaaattga actcttgtgg aaccaatcca
    74041 atctgttgct ttgctcgaac tagatccttg tccagatcat aaccgaagac attcactttt
    74101 ccagatgttt tattgactaa agaagtaata atcccgatcg ttgtagactt cccagctcca
    74161 tttggcccta atagcgcata gaaatctcct tctttcaccg aaagatcgat tccccgcaat
    74221 gcttcgaccc cacttccata gatctttttc aaattattta tttcaagcgc atttgccatt
    74281 tatttcctat ctccttatag acatgattct taattgtatc atggaataca gcagatgaaa
    74341 aaacaacttg gcttccacac aaaaacacac ctgctaacaa agaggagatg tgttttctag
    74401 ttcatgatca tcttcgatag aataactatc taaacaactc tggaactttt aaaatcataa
    74461 aagtaccgac agcaactact acgatgacat atagaagatc cttataactg aggttcttgt
    74521 cttttttatt catgttaagc tttcctccgt ccttttatcg aaagcttttt tcttttattt
    74581 taaccgaaat aaataaaatg gaaatagtta ttttcaattt ctgttttcgc tgtttgtttt
    74641 acggagaact ttgccatgga acgtgcgtct cgtcgctgat gctaagtttt ttcgataaaa
    74701 gctgctagaa ggaagacagc gtggtttatt ttgtatcgtc ttttgctaaa tcttccaaga
    74761 tcatctggac agcagactcg ttcccattga attctgccac ttttttggca tcaatgtact
    74821 tcttttgata atcgtctttt tggacccacg catcatgctt cgtttgataa agttaatcaa
    74881 acggatacca ctgacgctgt tcatcgaaca acaaactttc tttttgggcc tgttcaacta
    74941 tggatttagg ataattcaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc
    75001 cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt
    75061 ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa
    75121 atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt
    75181 cgatcgtatt tgttcctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag
    75241 ccacttggtc cagcaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt
    75301 caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact
    75361 gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga
    75421 catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga
    75481 ctggattaac taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc
    75541 cctcagtaaa ctctggtcgg caagtgatcg gcatataggt gcgataattc aaaacggctg
    75601 ctgcaacttc caattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt
    75661 ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat
    75721 attcaaaaat caattctgct tcactcccgc tcttcattcg aaatgaaaaa cctaatttag
    75781 ggatgtctct taatggttta gtttgctgtt tcagctcttt ttgaagtggt gtcgtcattt
    75841 ttgctaactg attggcggct gaaatcgttg aaaccaaata actcatgcta ttcagctctg
    75901 tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaag ccaccgattg
    75961 ctagatagat tcctagtact tgccctaaaa gaagcaaacc tacacccacc agcggaagca
    76021 agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat
    76081 attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat tggattcttt
    76141 ctctcagttg aggattttcc tgataaaaag caattagttt ttctaactga taatcttctc
    76201 caaaatcaaa gctacgtaaa cgctgataca acgcttctga acctacacta gaataggtac
    76261 tgttgatccc ttcaaatact tcgaacatat ccaagtcata ccaagtaaga tcgtcgattt
    76321 cactatccca tgaacggaat tttttctccg tgagccacgc atccttcaaa ctttcttctt
    76381 tatcaaatct cggttgataa ggaatttttc cccaattact acgaactgtt tcctttactt
    76441 tcaaacgtga aaaaatgtct aaaacaacta ataaaataac aactgcaata ataattaaaa
    76501 cgataaattc tctttggccc atatccctct ccgttccttg atttgttagt tttagtatag
    76561 cactgttgcg ctatttgaaa agggggtaca aaaataactg tcttcgttac ctaattcgta
    76621 tcaatcacac caaaataaaa acaccttcca aaaaagaaga tgtctttaaa acgagataca
    76681 attttttgtc attcacttca ggtaaattag agccaattaa accctccgca acttgacgga
    76741 gatggagaag atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa
    76801 tttttttgac aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc
    76861 ggtataaaaa cgctggaaga ctttttcaac agaggtttgc gctcctggtt tgataggatt
    76921 ggccactgtt aatcgaatga atgaatcctc ttttgaaaat gtcacctttg cttgtccatc
    76981 tgcataacgc aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc
    77041 tgctaataca gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga
    77101 aaattgttca taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg
    77161 gagatccatt tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat
    77221 actgagtaat cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt
    77281 atcctctacc tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag
    77341 atcatgagaa aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg
    77401 tcctttttgt tcattttcat ccacccatgt attcagtgca tctactaggt ccattagatt
    77461 cttttctcga aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc
    77521 gattttctta gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa
    77581 gataacaacg agaagcaaca gccacttaat caaatctaaa ccccactccc cacaccgtat
    77641 gaatatacac agcatgtgcc tttgctagtt tccctcgaag attggaaata tgcatattca
    77701 ctgttttctc actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg
    77761 aaaatacttt tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca
    77821 aatggacagc cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct
    77881 tgtaatagat gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga
    77941 tgccgattcg taacaacagt tcttcgatat caaatggttt cgtcacatag tcatccgctc
    78001 ctgctgccaa caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg
    78061 gaacattcga tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca
    78121 tgagatctag aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac
    78181 ctgaataagc atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaagaatgaa
    78241 cagcttcatc gtcttcgata atcaaaatag tttccagcag gaacacctac ttaatgtcag
    78301 atttttggaa aataaacatt cctagagctg atgataccac gattgttact agactgacca
    78361 gaatcatttt cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc
    78421 acgtttccgt aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg
    78481 gaacgaaggc cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc
    78541 tagtgatgaa tgtcaaagcc actggaacag ataatgtcgc taatatcagc agaagcaata
    78601 caggaaatac ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc
    78661 cgaaagcaag tgtgtaagca atcatggcta gccccgtaat caaaaccacg ccaagtgttg
    78721 tgatcaaaag cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag
    78781 ataacgtatt gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg
    78841 ctgcgaaagc cagaagaatc catgcagcca ttttttccac cattaaaatc cctgttgcac
    78901 cgttgatgac tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc
    78961 attcgttcca agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt
    79021 tacggctctt ccatactttt attttttcag cagttaatag attaaacatg tggattgcct
    79081 ccagttaatt gtaagaaata atcttccagt ttttgcccaa ctaggtggat accatccaca
    79141 ggaacttttg cctctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa
    79201 agctggataa cacggctaga ttggacaaaa taatccctat aaccaagaga gtcgagaacc
    79261 gttactgctt gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc
    79321 aattcttctt tcgataattc tttgatcaag tgtccttcat gcaaaatacc gtagtgagtg
    79381 gcgatctgtg acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct
    79441 tttaccaaac gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc
    79501 gtcggttcat ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa
    79561 cgctggcgca tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc
    79621 atcattttca gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata
    79681 cgtaggttat ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc
    79741 atacgtcgtc ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca
    79801 atcgagatca aacccataat agttctcata aaggtagact tacccgctcc attctcccca
    79861 atcaaaccat agatcattcc acgtttgatt tcgatcgata ctttatctaa tgctttggca
    79921 gcaccgtatt ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata
    79981 cttatccttc tttcttattg ataagtgaag tatagttgac gggtataaag aaagcagcca
    80041 gaaatgtata aagaaagtgt aaattttcca attgaatttc aagaaaaatc agactgtctc
    80101 gcagacgtgg ttgagttgtc tgccaatcaa agagcgcacc aaacagtgct aatccaagtg
    80161 gactggccac catggtaatc gttgttatcg ttgacatcag acgtccttgt atatgcgttg
    80221 gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac
    80281 ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt
    80341 tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc
    80401 ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt
    80461 atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga
    80521 tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc
    80581 tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag
    80641 aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac
    80701 tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt
    80761 tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaagcct tgatttataa
    80821 aggtttttcc gtaaatatat tttgttatat ttgattaaat ttgaaatgtt tgtgttctaa
    80881 aatgtgttct aatttaaagg cagtatattt cgtattttat attaaatttt taggctcatt
    80941 cattctcaaa tccattctat ttagtcaata aatattttaa aaagacttta taaacaaaga
    81001 tgttcaagcg agcatcttat ttttttgtgc ctaatttgaa aggagaacat ttcatgtata
    81061 aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt caatcaattg
    81121 taggagcaat tgctcctacg attgcttttg ctgatgaaat tacccatcca caaacggtaa
    81181 ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt gatggtcgtc
    81241 caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggcgttaaa caagatattt
    81301 tctgtgttga accaggagtc ccgatttaca atgagttcac tcctggatat gagaagaacc
    81361 cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa aaagtaggta
    81421 cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt aatgggtata
    81481 cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc attgaggcaa
    81541 aaatcaatca agccattgcc gattatcaga agaaaccaag cttccataat agtacggcta
    81601 aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat ttgtcagagt
    81661 tcgatgaggt tgtggaaaat accgcaaaca ttgattatcg tgtgaatggc aatcagttga
    81721 taatcagccc aaatgccaat tcaaaagaaa gtggtgtgtt gacgcttaag aaatctgctg
    81781 gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg gccggggcga
    81841 tcgataagcc gaacacttat acggtcaaaa ttgatgtgga aatggagggt tctttgaaga
    81901 ttaaaaaagt tgataaagaa tcgggtgata ttataccagg aacggttttc catttagact
    81961 ttggaaaaac tttacccgca aaagacgtga ccaccgataa agaaggcatt gcgaccttag
    82021 atgagattcc tcatggtaca aaggtaacca ttactgaaaa atcggtgccg gcgccttata
    82081 cgattgatac cacacccatg actgccacaa ttaaagcagg tgaaaccatt tcagtgactt
    82141 caaaaaatac gcgagaaaaa ggtcaaatta ttctggataa aaccggaatt gaaacaggga
    82201 gtgatctttg gaacgataat tactctttag cgggcaatac gtttgccatt cgcaaggata
    82261 gcccaactgg tgaaatcgtc caagaaatga ccacggatga aaaaggtcat gcagaaacac
    82321 caaaagagat tgccaatgcg ttggaattgg gaacctacta tgtgactgaa accaaggcta
    82381 gtcatggctt tgtgaatact ttcaaaccag taaaagtcga attgaattat gtcaatcaaa
    82441 cagtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaattact ggtgaaacca
    82501 ctttgacaaa agaagacaaa gataccggtc atgaaactca agggaaagct gagtttaaaa
    82561 gagctgaata caccttgttt actgttaagg atggaaaagc ggtcaaatgg accgagaact
    82621 ttaaaccaga attgttgaag ggaacgaaag cttctgatga aaccgtaact ttgactttag
    82681 atgaaaagaa tcaagttgcc gttaaacact tagcgattaa tgagtattac tggcaagaaa
    82741 ccaaagcgcc tgaaggatat accttggatg aaacaaagta tcctgtatcc atcaaaaagg
    82801 tggatgatag cgaaaaaaat gctgtgatta ctcgagatgt tgcggcaaaa gaacaagtga
    82861 ttcgctttgg ctttgatttc tttaaatttg ctggatcaac tactggcact gccgaaactg
    82921 gcttcaatga tctaaccttt aaagtgtcgc cattggaagg aaccaatgaa atcaccggtg
    82981 ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt tatggcaagt
    83041 ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca gaaggttttc
    83101 aaaagattac gccattagaa atccgttcta cttttaaaga aaataaagag gattatacca
    83161 agagtgatta tatctttact attaccgaaa aagggcaaaa gcaaccgatt aaaacggtaa
    83221 cggtccctta tgggaatcta accaatgaga atttctcagt cagtttgaac cgtttgatgc
    83281 tttatgattt gcctgaggaa gaagatagct taacttctct tgcgacttgg aaagaaggag
    83341 ataaagaatt gactactctt gattctaccg agctagtcga taaattgagc tataatctac
    83401 atgaaatcaa agaagactgg tatatcgtag ctcaagccat cgatgtagat gctacaaaag
    83461 ctgcccaaga aaaagataaa aaagccaaac cggtggcgat tgcggaaaca accgcaactt
    83521 tagcgaataa agagaaaaca ggaacttggg agattctgca taaattaact gtcgaacaag
    83581 ttttagacaa gaccattgtc ttattcaact atgtgtatga aagcaaagaa gcctatgaag
    83641 ctggtgatga gccggtagcg aaggatgcta gcttgaacaa tcaagcgcaa actgttaatt
    83701 gtatggtgga acgtcatgtt tccatccaaa ccaaagcgca cttagaagaa ggttctcaga
    83761 cctttactca tggtgacgtg gtagatatgt ttgatgatgt atccattacc catgatgtac
    83821 tagatggctc aaaagaagct tttgaaacaa ttctgtacgc tttatttcca gatggtacaa
    83881 gtaaagaaat ttggaaatct ggcaagattg attacgaagt gaatgacaaa gaatttacca
    83941 aaaccgtact tgcggaaaaa gtggatacaa gtaaatatcc agaaggtaca agttttacct
    84001 tcaaagaaat taactatgac aaagatggga atatcaatgg aaaacacaat gaagatttga
    84061 aagaaaaatc tcaaacttta acgcccaaaa aagttccgac aacaccaagt acaccagaac
    84121 aatctgaaat gccaactgtt ccaagtggct ctcaagaatc tagtcctaca gtgaaggcat
    84181 tccctcaaac cggggagaaa acttccaatg tgctactgtt cattggcttt agcttgatct
    84241 ttgcgacggc tggctattat ttttggaatc gtcggaacta aggtgataag atgaaacatg
    84301 taaatagtag aaaacaatac gcgcgaccgc ctcctaaaaa agtggtcgcg ctatttttgt
    84361 attcaaaact aacaatgaaa agaggaaaaa atatggaatt gaaatttgtt gtaccagata
    84421 tggtcgagac gttcggaaaa ctaacatacg gtggtgaagg tgaagttcta tcggaagggt
    84481 atggtcgaaa tactacaatt gttggtcgaa gttaccatct gttttcaaat aaacaacggg
    84541 ccgatgatat tgaagtggtg gtagctgcgg aagccggcga aaaggatttt gaccaagatc
    84601 aaccactaaa agctgtaaat ccccacttaa ttgccaaagg ctatgaaatt gagaatcgtg
    84661 ggtttaccga ttacgtgttg tatgttgatg atttagtgaa agcataggag gaaaagaaga
    84721 tgagattaac agaaggtatt gtcgtagatt caggattaac ctttggcaag ttgcgttttt
    84781 ccgcattacg tcgagaagta cgcaagcaaa atgaggacgg aacggttagt aacgaggtaa
    84841 aagaacggac ctataatttg aaatcctccg cgcaaggtcg aatgatccaa gtcagtattc
    84901 cagcaaatgt acccttacgc gagtttgctt atgatgcaga ggtagagctg gtcaatccaa
    84961 tcgtggatac ggtcgccaat tatgtttttc gggaaggaac caccgttaat tggtttatca
    85021 aggcagatga tttagtcttg aaacgacaac tgaatcaagg aaaacctacg aaccaaaatg
    85081 aaggaaagaa ataggtgaag aatatggaaa ttaatgttga acatatttta gattgtctcg
    85141 atcaatttgg caaaggtgag ctaacggagg aacaactcac taacgcatta acctatgatg
    85201 aaaaaatgtt tctgattatg catcaagggt tactggaagt aggtaacgat gccaaagaag
    85261 actttgatgt actggattgg ttaggggaag aagagtcttt ctttatggtt gtcgaggtaa
    85321 atgaaagttt atgtcggcag gcagaatctg ttttggaaga aatcgatgtg gagatgccgg
    85381 atgccatcga aagattttta aaacaattag tggaaacgaa acaacttccc gtagtagtaa
    85441 atgactagca taactttttg aaattgccca atgaaatcct attgggcttt ttcgactgga
    85501 ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc gcaggagatc aacacttgat
    85561 ttatcacttt gttcttggct ggctaagtgc cttattcatc ggttggatgg gcgtctttta
    85621 ttttccagag cttagacaat ttggtctttc taaggtatca ttttccacta ttgaaatcgc
    85681 ttggtccatg aaagatttag tatgtctgct tggaagtctc gctctttcag gaagtatgat
    85741 gttgctttat attcattttt ttcctgatca ttggcgaagt ttgtggaatc gacaaaagct
    85801 agccagaatg attctagaaa accattggta cgaagtgaag caaactcaaa gtgatggctt
    85861 tttcaaagat ttgaatagga gtcgaaccaa agagactatc agttacttcc ccaaagttta
    85921 ttatcgaatg aaagatggct tactgtctat tcgcgttcaa atttcactgg gaaaatatca
    85981 agatcagcta ttgaagttgg aaaagaagtt agaaagcgga ctgtattgtg agttagtaga
    86041 aaaggaactc aaagactcct atgtggaata caccttgtta tacgatatga ttgccaatcg
    86101 aattgggata gaagaagtag tggcagaaaa tgggacgttg cgattgatga aaaatcaagt
    86161 atgggcgtat gattccttgc ctcatatgtt aatcgctggt ggcacaggtg gtgggaagac
    86221 ctatttctta ctcaccatta ttcaagcact attgaagtca gatgcagaat tgtttatcct
    86281 cgatcccaaa aatgcggacc tcgccgattt aggtacggta atgcctcatg tctattctca
    86341 aaaagaagaa atttctaaat gtgtggaaga tttttacgaa cgcatgatgg ctcgtagtag
    86401 gtcaatgaaa gaaatgccca actacaaaac aggagagaat tatgcgtatc tcggacttcc
    86461 gcccaacttt ttaatctttg atgaatacgt ggcttatatg ggtgcgaatc gttttcctac
    86521 aagcatcgaa tagatgtgaa atggaagcct attgagatta tcaatagtga caactaatgt
    86581 tttgagagat ggaatgaaag ccttcaagtt gagctgaaac atccccgcta atactcctac
    86641 atgctctaat caatgaagta gattggtttt ggtatgaagc taggtgaagt cgtctgaaat
    86701 ctgcctaagt gcttgagcat aaggctgact atagggcggg cggctgaaaa agataactat
    86761 ggtaagaatg tctgaaaatg actgacgaat ctgcgaatgt acgggtctaa aaggctgatc
    86821 ttattgaaag ataggaacat acggacgtat ggtgagatac cgacaagtaa ggttccatga
    86881 tatgaaaatc gggatagcta acgaagaagc tataatttca caggctcata gtagatacct
    86941 aaggttatac atgatagcta aaaacattgg aacgatgtaa gcagaaaact atcaattaaa
    87001 tgactacttc tagataggtg catataagta ataacgaaat tgctttgttt ctgtgaaagt
    87061 aagggcacag taccgatgaa gcgtctaaca aatgtggagg gatagcccta agtctattta
    87121 ataacaatga atgttgaaac agatgaagtt caaggatctg gtaagaacgt gggaaaatcc
    87181 gttatggagg tgccacgctt gaacacaaaa atacgttact gggaatatta tgatctacaa
    87241 gaaacattcg atcagctatt tactcaaagt agaaacggga aaaagtttta tcaattatat
    87301 gagttaatca tttctgaaaa taatattctt ttagcatacc gtactatcaa ggcaaacaag
    87361 gggtcttcga caccaggaac ggattccttc acaattgata actataagga aatgaaccaa
    87421 gcagagttta tccatcttat tctcagtcat ttagaaaatt acaaaccaaa atccataaag
    87481 cgagtaatga tacctaaacc caatggtgag aaacgcccat taggaattcc atgcatgatt
    87541 gatcgaatga tccaacagat gttcaagcag atactggagc caatatgtga agctaagttt
    87601 tatgaacata gctacggttt cagaccattg agaagtgcaa agcatgctct aggtcgcatt
    87661 atgtatctca taaacatcag taaaatgcac tatgcagtag acattgatat caaaggtttc
    87721 tttgataatg taaaccaccg tttactgata aaacaattat ggaatatagg aatttgtgac
    87781 aaacaagtct tagctattct ttcaaaatct ctgaaatcac ccattcaagg agaaggtatc
    87841 ccgagcaaag gaacgatcca aggaggtatc atttctccac tattatctaa tgttgttcta
    87901 aacgacttag accattgggt ctcaaagcag tggcatacct ttgaaacaaa gtatccctat
    87961 actaaaggtt acaataaatt tcgtgctcta agagatacaa atctaaagca aggctatatt
    88021 gtcaggtacg cagatgactt taaaatcatg actaatgatt atccgacagc attaaaatgg
    88081 ttccacgctg tcaagcttta cctaaaagac agacttaaat tagatatttc caatgagaag
    88141 tctaaaatcg tcaatttgcg taaatgtaag tcggaatttt taggattcgc tatttgcgtg
    88201 aaacaaaaag ggaaaaaatg ggtttgtaat tcacgtattt ctaacaaaaa aaaggatcag
    88261 attaaagaag agattaaaca aagaatcaaa gatattcaaa agagtcctac agcacaaaat
    88321 gctctcctgt ttaatgctct cgtgctcggt gttcataatt atttcaggta tgccactcat
    88381 attaatttag atttgaatag gatgaactac gttctttcat tcactactta caaccgatta
    88441 tcaaattgta gtacaagatg ttaccccaac aaagcttccc caacttacaa aaagttttac
    88501 tcaacaacga tgaaaacgta taaagtagct ggagtctatt tgtaccctct ttgcgacgta
    88561 tctacaaaaa caatttacgg atttaatacg gaggatacac cgtttactcc gtttggtcgt
    88621 caaagattgg agcacaaaag cctacagtct cttgtgttcc aagaattaag aaaattgatg
    88681 gaatcgaaaa taccgaatag aacggttgag tatttagata gtagaatatc tcgttactca
    88741 atgaaatcag gcaagtgtga gattacaaaa caatttttgc cagcaaaagc cgttcactgt
    88801 catcactatt tgccaaaaag tcttggtgga gacgacaaat tcgataactt gagaattatt
    88861 cataaggata tccatcttct gatccataca acaaataaaa tgataatcga tcactacgtg
    88921 catgaactga aactggtgcc agagcaaata gcaaaaatca acctttatcg aaagatgtgt
    88981 aatctgcaaa acattcaata gaaaataaat agatggaacg ccgtatgagg tgaaagtctc
    89041 acgtacggtg tggagcgggg gaaaagatgg agattatatc aaattcttac ctatcgctat
    89101 agatgttaac gacaaaagaa agtgcggtga ttttgaataa gcttaaacaa atcgtgatgt
    89161 taggccgtca atctggtttc tttctgattc tagcttgtca aaggccggat gcgaaatatc
    89221 taggagatgg gattcgcgat cagtttaact tccgtgtggc tttgggtcgt atgagtgagc
    89281 ttgggtattc catgatgttt ggagaagtcg ataaaaattt ttttatgaaa cgcatcaaag
    89341 gacgaggtta tgtagataca gggggcagtg tgattagtga gttttatacg ccacttgtac
    89401 caaaagggta tgatttctta agggaaatta gcaacattgt tggattaacc gttcatacgg
    89461 aaagagaaaa taatagtgtg tagcctagga atatcaataa tcctgggctt ttttattgag
    89521 gtgatttatg acatttttag atttattcgc cgggattggt ggctttcgct taggtatgga
    89581 acaagccggt caccagtgta tcggtttttg tgaaattgat gaatttgctc ggaggagtta
    89641 taaagcaatc catgatacaa ggaaggaggt ggaaatgcat gacatcacaa gtgtatcaga
    89701 cgaatttgtt cgatccctcg gaccagtgga catcctttgc ggcggctttc cgtgccaagc
    89761 tttttcaatt gcgggaaagc ggcaaggatt ttccgatact cgaggtactt tattctttga
    89821 aatcgctcgg ttcgccactc ttctccaacc taagtttcta ttccttgaga acgtccgggg
    89881 attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgt tggatggatt
    89941 ggggtatgat gtggaatggc aagtgcttaa ctcaaaggcc tacgttcccc aaagccgtga
    90001 gcggatcttc cttatcggac attctcgaga tgcatgtacc gaacaagtat ttcctatcat
    90061 tggatcgtct tcaacatctg atcaaaacat cagaaacttg gtaaatatca atccttccaa
    90121 tcgaggaatg ggtggccaag tctatggttc agatagcgta gcaccaacct tgaccggtga
    90181 tgaaggaatt aaaattgcct taccggtaaa ggatgggatt tctgtagcgg gtatgttgtc
    90241 gggaaatttt gaacaaggca ataggattta tgaggtaacc ggaacagccc ccactttgtc
    90301 aacgaaacaa gggggaacga agattatgat tcggaaaaat gaaacagaat atcaagaggt
    90361 gaagccaggg gacagtgtga acctggcttt tcctaattcc acaagtcgcc gaggtcgtct
    90421 gggaaaacaa agtgtgcata ccttactaac aggagaccaa caagcggtag tcacggatca
    90481 gtaccagatt cgaaagttaa cacctaggga gtgctggcgg cttcagggct ttccagattg
    90541 ggcttttgat cgagcttctc aagtcaattc agatagtcag ttgtataaac aggccggaaa
    90601 ctcagtaaca gttccggtca tttttgatat tgctagaaga ttaaaggggg taaaagcata
    90661 tgatttatga cgagttgatt ggagaaatct actgggtgat tggcaagatt caggcagatc
    90721 cgaaattgga agaagaactg taccggctca attttgatat tcgcaaaaac ggggtgaaag
    90781 tccaggtgat tcgtatgtag tggatgagga aacggatgct cgaatggagc tcaatcaagt
    90841 catcggtgaa ttggaacgaa ttgcggacct tgccaaagaa cctgatatcc gccaatactt
    90901 gtttgagata aaagcagagt tggaaattga gggtatcacc gcggagtaat agggaaataa
    90961 cccactgata ggcactgacc gcgggggatt tgcgaagcag aaatcctccg tggtgctgtg
    91021 cctgttcatg gacggcggca gccggccatg atgtctcacc ccccgtatct aacagggggg
    91081 tacaaaaaca caaaaaacaa gcaagtaacc ataaaaaatg cttgttgtat caaggatatt
    91141 aagtaatttg tataggtgtt aactcatttg gaaaagttaa caccttgaat ttggaggtga
    91201 aaacttggcg caacgaaatt tagattatcg gttattaaaa gaacgacgca acgaatatgg
    91261 cgtttcacaa aataaattag ctactgcttg cggacttagc cgcccatacc taaatcagat
    91321 agaaaacggt ggcgtaatcg cctctacaaa gaccatgaga aaaatttttg atcaactgga
    91381 aagtttcaat cccgatttac ctttaacgtt actatttgac tatgtaagga ttcgctttcc
    91441 cacaacggat gtgcggaaaa ttattcaaga aattctccat ttgaaattcg agtatatgct
    91501 tcatgaagat tacgcctttt actcctatca agaacaatat gtcatgggcg atattgtagt
    91561 gatgttatct catgaagaag ataaaggtgt ccttttggaa ttaaaaggtc gtggttgccg
    91621 gcagtttgaa acttttttac tcgcccaaaa gcgtagctgg tacgactttt ttgaagattg
    91681 tctaaaagcc ggtggcgtga tgaaacgctt ggatttagca atcaatgatc gagtggggct
    91741 actggatatt cctgatttaa cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg
    91801 taccttcaaa agttaccgtt ccggggaact gttgaaagct gatgaaaagg atggtatggg
    91861 caatacctta tatattggca gcctcaaaag cgaggtttat ttttgtttgt acgaaaaaga
    91921 ttatgaacaa tatatcaagt tagggattcc attggaccag accgaaacga aaaatcgttt
    91981 tgagattcgg ttgaaaaatg accgtgctta ttatgcgatt caagatttgt taaaaggtcg
    92041 tagcattgaa agtaccacgt tttccattat caatcggtat ttgcgatttg cggataaagt
    92101 agatggaaaa cggcgaacca attggccctt gaatgaacaa tggggtcgtt ttattggacg
    92161 gaatcggaaa gagattcaat taacttctga accgaaaccg tataccattg aacgaacatt
    92221 aaattggcta ggtagacaag tcgcccctac gtggaaaatg gctaaggagt tggatcgatt
    92281 gaatcaaaca acgtatatcc aggatatggt acagaatgcg cgactctcgg accggcataa
    92341 aaagattttg gagcaacaaa gtatggcaat cgaaaacttg attgtatgaa aagaggaatt
    92401 ttgatggaaa ttgtaaaaga tatgttattg gtacttggtg gcagtatgct tggtgttacc
    92461 gtgatgtgct tgatgaatgc aagtgcagca gcggattgag caatggaaaa gaaaggaaag
    92521 tgacaaaatg aatttcggac aaaatttata tcaatggttt ttaaccaatg ctcaatcttt
    92581 agtcttacta gcgattgtgg tcattggttt gtttttagga tttaaacggg agttttccaa
    92641 actgattggc ttcttggtga ttgctttgat tgcggtaggt ctggtcttta atgcttctgg
    92701 tgtgaaggat gtcctactca atttgtttaa ccgaattgtc ggtgcctgag ggggaatcac
    92761 aatggaacag atgcgtgttt atattgcgaa ccttggaaaa tacaatgaag gtgagttggt
    92821 gggggcatgg tttaccccac ctgtcgattt tgatgaagtg aaagaacaga ttggattaaa
    92881 tgacgaatat gaagagtacg cgattcatga ttatgaactg ccttttgaga ttgatgagta
    92941 tacgccaatt gaagagatta atcggttgtg taacttagcg gaggaactag aaggaacgcc
    93001 aattggtgaa gctgcttctg agattcaaca cgctttcttc aattctttcg aggaaatggt
    93061 agagcatgtg gatgatattg tctattatcc agattgcgat gatatgagtg atgtggcgca
    93121 ttacttgatt gtagaagctg gtgattttgg cgaagttcca gaacaattga agccgtatat
    93181 tgattttgat gcttgcggtc gagatttgga gattggagga aattatttgg ttactagtcg
    93241 tggagttttt gagtatccag aatgaatcaa acactatata tagtgtttga ttagtatatg
    93301 taagcaaaaa aacacaatat atagagtgca aactgaaggg taatgtgata taatatttgc
    93361 cgaagaaata tttttgaaat gaggtaatat tatggcaaat attcaaacaa gttttattga
    93421 tttccataat tcaattagat tggacgtaga agacaatacg ctacttaagg actataaaga
    93481 ccaagtaatt gatggattaa aagattactt acctgatgat gtgaagtttg aaactttttt
    93541 acagggaagc tattctgttt ataccggaat aaaaagctgt gatgaaaaaa ttgattttga
    93601 catcgatatt gcagttgctt tcgaaattga tcatactgtt tatgaagatc caagggagcc
    93661 taaactgtgg gtaaaagagg cgctagttga aatttttcct aatgctcaag ttaatttaaa
    93721 agtaccttgt gtaactgcta catttactgg aaaaaagaca aaaaaaaatg tgcacgttga
    93781 tgttgcggtt tatgctaaag aagatgaaaa ttatttctta gcaaaagcaa aagaattcag
    93841 tgctcccgaa aaccgatgct gggaggaagc ggatcccaaa gtattaaaag agaaaattaa
    93901 tagtcatgta gcagattcag atgatcgaaa acaatttcga agatgtattc gctatttaaa
    93961 aagatggaaa gataacaatt ttaaccaaga atataaacca acaggaattg gacttacaat
    94021 taatgtgatg gatacgttcc tagttaataa gtccactgat tttttaacta gaaaagttca
    94081 gtataatgat atggaatgta tgaagcagat tgtttcttcg ctgaaagatt catttgtgta
    94141 tgagtacagt gaaacagatg gttggcatta tcgtctacat gcatatcttc cagtgaagcc
    94201 taacagtgac acctattcaa aaatgacagt gaatcagatg agtgatttta aaaacaaact
    94261 ttctaaatta tatgatgatt tgattttcgc tatcgatact gaagatgagt atgaagctac
    94321 aaaacgatta aataatcaat ttggggagga ttttttaatt atttcagaag aagaagttac
    94381 agaaaaaaac ttaagaaatg catttgttac agactatccg agtgcttagg ttggtgttaa
    94441 agattggatt atactgatat tttaaaagaa aagctaacag atgttgtgcg tttattcgat
    94501 ggtaattact atggggattt ttcatttgct acaattggaa agttgcgttt aaaaattgaa
    94561 ataccaaaac attttccatt ggtacttcct aggatttaca ttcataattt tgatcattta
    94621 ggactttacc taccacatgt agaaaaagat gggttggtat gtttttcttc agatataaat
    94681 tatttagtag atgaagaaaa ttcagaattg gttttattgc agtgtgttaa taaaactata
    94741 aaaacaattg aggaaggttt gaaagggtta aataaaaatg attttcgaaa agagtttgtt
    94801 gctttttggg acaaacaaga gaatcaaatt tcgttattct acctttgtga accagcgttg
    94861 tttatcaagt cagttgatac aataatgtac ggtaagtctt tggttgcttt ggataaagag
    94921 aacgaattac aattaaatgc agcgagtaga ttgtttaatg gagagaaaga agatactcat
    94981 gaattagatg catactatgt tccaattaga aacaagaata atattatgcc cccgaagaat
    95041 aataaagcat ggtcaaagaa ggata
//
LOCUS       DACDIP010000005        79802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDIP010000005 DACDIP010000000
VERSION     DACDIP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.005"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IV257_000392"
     CDS             591..1280
                     /locus_tag="IV257_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8813548.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IV257_000393"
     CDS             1267..2475
                     /locus_tag="IV257_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8813549.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IV257_000394"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IV257_000394"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8813550.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV257_000395"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV257_000395"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8813551.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IV257_000396"
     CDS             complement(6578..7273)
                     /locus_tag="IV257_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8813552.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV257_000397"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV257_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8813553.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IV257_000398"
     CDS             complement(8472..8663)
                     /locus_tag="IV257_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8813554.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IV257_000399"
     CDS             complement(8684..9574)
                     /locus_tag="IV257_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8813555.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IV257_000400"
     CDS             complement(9561..10328)
                     /locus_tag="IV257_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8813556.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV257_000401"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV257_000401"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8813557.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV257_000402"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV257_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8813558.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV257_000403"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV257_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8813559.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IV257_000404"
     CDS             14944..15282
                     /locus_tag="IV257_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813560.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IV257_000405"
     CDS             complement(15450..16208)
                     /locus_tag="IV257_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8813561.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IV257_000406"
     CDS             complement(16226..17044)
                     /locus_tag="IV257_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8813562.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV257_000407"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV257_000407"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8813563.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV257_000408"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV257_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8813564.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IV257_000409"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IV257_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8813565.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IV257_000410"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IV257_000410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8813566.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IV257_000411"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IV257_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8813567.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IV257_000412"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IV257_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8813568.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IV257_000413"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IV257_000413"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8813569.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IV257_000414"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IV257_000414"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8813570.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IV257_000415"
     CDS             27086..27385
                     /locus_tag="IV257_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8813571.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IV257_000416"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IV257_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8813572.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IV257_000417"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IV257_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8813573.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IV257_000418"
     CDS             28352..30325
                     /locus_tag="IV257_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8813574.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IV257_000419"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IV257_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8813575.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IV257_000420"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IV257_000420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8813576.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IV257_000421"
     CDS             33204..34496
                     /locus_tag="IV257_000421"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8813577.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35283..36146
                     /locus_tag="IV257_000422"
     CDS             35283..36146
                     /locus_tag="IV257_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8813578.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36275..37897)
                     /locus_tag="IV257_000423"
     CDS             complement(36275..37897)
                     /locus_tag="IV257_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8813579.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38146..38667
                     /locus_tag="IV257_000424"
     CDS             38146..38667
                     /locus_tag="IV257_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813580.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGTIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="IV257_000425"
     CDS             complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="IV257_000425"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8813581.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40748..41566
                     /locus_tag="IV257_000426"
     CDS             40748..41566
                     /locus_tag="IV257_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8813582.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41668..41865)
                     /locus_tag="IV257_000427"
     CDS             complement(41668..41865)
                     /locus_tag="IV257_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813583.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42034..42612
                     /locus_tag="IV257_000428"
     CDS             42034..42612
                     /locus_tag="IV257_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8813584.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43657..44160)
                     /locus_tag="IV257_000429"
     CDS             complement(43657..44160)
                     /locus_tag="IV257_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8813585.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44519..45544
                     /locus_tag="IV257_000430"
     CDS             44519..45544
                     /locus_tag="IV257_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8813586.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45867..46745
                     /locus_tag="IV257_000431"
     CDS             45867..46745
                     /locus_tag="IV257_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813587.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47221..47337
                     /locus_tag="IV257_000432"
     CDS             47221..47337
                     /locus_tag="IV257_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8813588.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48265..48603
                     /locus_tag="IV257_000433"
     CDS             48265..48603
                     /locus_tag="IV257_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813589.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48697..48927
                     /locus_tag="IV257_000434"
     CDS             48697..48927
                     /locus_tag="IV257_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813590.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49327..49698
                     /locus_tag="IV257_000435"
     CDS             49327..49698
                     /locus_tag="IV257_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8813591.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49972..50664)
                     /locus_tag="IV257_000436"
     CDS             complement(49972..50664)
                     /locus_tag="IV257_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8813592.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50667..51452)
                     /locus_tag="IV257_000437"
     CDS             complement(50667..51452)
                     /locus_tag="IV257_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8813593.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51689..53089
                     /gene="lacG"
                     /locus_tag="IV257_000438"
     CDS             51689..53089
                     /gene="lacG"
                     /locus_tag="IV257_000438"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8813594.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53114..53431
                     /locus_tag="IV257_000439"
     CDS             53114..53431
                     /locus_tag="IV257_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8813595.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53444..55117
                     /locus_tag="IV257_000440"
     CDS             53444..55117
                     /locus_tag="IV257_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8813596.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55131..56762
                     /locus_tag="IV257_000441"
     CDS             55131..56762
                     /locus_tag="IV257_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8813597.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57253..58818)
                     /gene="guaA"
                     /locus_tag="IV257_000442"
     CDS             complement(57253..58818)
                     /gene="guaA"
                     /locus_tag="IV257_000442"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8813598.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(58877..59800)
                     /locus_tag="IV257_000443"
     CDS             complement(58877..59800)
                     /locus_tag="IV257_000443"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8813599.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            59996..60601
                     /locus_tag="IV257_000444"
     CDS             59996..60601
                     /locus_tag="IV257_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8813600.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            60909..61925
                     /locus_tag="IV257_000445"
     CDS             60909..61925
                     /locus_tag="IV257_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8813601.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62066..63367
                     /locus_tag="IV257_000446"
     CDS             62066..63367
                     /locus_tag="IV257_000446"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8813602.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63413..64075
                     /gene="deoC"
                     /locus_tag="IV257_000447"
     CDS             63413..64075
                     /gene="deoC"
                     /locus_tag="IV257_000447"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8813603.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64096..64494
                     /locus_tag="IV257_000448"
     CDS             64096..64494
                     /locus_tag="IV257_000448"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8813604.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64560..65642
                     /locus_tag="IV257_000449"
     CDS             64560..65642
                     /locus_tag="IV257_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8813605.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            65854..67416
                     /locus_tag="IV257_000450"
     CDS             65854..67416
                     /locus_tag="IV257_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813606.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67409..68542
                     /locus_tag="IV257_000451"
     CDS             67409..68542
                     /locus_tag="IV257_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813607.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68542..69498
                     /locus_tag="IV257_000452"
     CDS             68542..69498
                     /locus_tag="IV257_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813608.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69597..70763
                     /gene="deoB"
                     /locus_tag="IV257_000453"
     CDS             69597..70763
                     /gene="deoB"
                     /locus_tag="IV257_000453"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8813609.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            70787..71605
                     /locus_tag="IV257_000454"
     CDS             70787..71605
                     /locus_tag="IV257_000454"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8813610.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71622..72329
                     /gene="deoD"
                     /locus_tag="IV257_000455"
     CDS             71622..72329
                     /gene="deoD"
                     /locus_tag="IV257_000455"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8813611.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72627..73313)
                     /gene="gpmA"
                     /locus_tag="IV257_000456"
     CDS             complement(72627..73313)
                     /gene="gpmA"
                     /locus_tag="IV257_000456"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8813612.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73485..74066)
                     /locus_tag="IV257_000457"
     CDS             complement(73485..74066)
                     /locus_tag="IV257_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8813613.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74199..74882)
                     /gene="rpiA"
                     /locus_tag="IV257_000458"
     CDS             complement(74199..74882)
                     /gene="rpiA"
                     /locus_tag="IV257_000458"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8813614.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75201..75614
                     /gene="rpsL"
                     /locus_tag="IV257_000459"
     CDS             75201..75614
                     /gene="rpsL"
                     /locus_tag="IV257_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8813615.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVRD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75698..76168
                     /gene="rpsG"
                     /locus_tag="IV257_000460"
     CDS             75698..76168
                     /gene="rpsG"
                     /locus_tag="IV257_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8813616.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGISANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76262..78349
                     /gene="fusA"
                     /locus_tag="IV257_000461"
     CDS             76262..78349
                     /gene="fusA"
                     /locus_tag="IV257_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8813617.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78521..79708
                     /gene="tuf"
                     /locus_tag="IV257_000462"
     CDS             78521..79708
                     /gene="tuf"
                     /locus_tag="IV257_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8813618.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT        24547 a        14803 c        16324 g        24128 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggagcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
    34741 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
    34801 aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa
    34861 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct
    34921 cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    34981 caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat
    35041 ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt
    35101 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    35161 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
    35221 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
    35281 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
    35341 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
    35401 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
    35461 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
    35521 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
    35581 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
    35641 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
    35701 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
    35761 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
    35821 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
    35881 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
    35941 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
    36001 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
    36061 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
    36121 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
    36181 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
    36241 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
    36301 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
    36361 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
    36421 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
    36481 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
    36541 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
    36601 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
    36661 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
    36721 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
    36781 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
    36841 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
    36901 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
    36961 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
    37021 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
    37081 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
    37141 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
    37201 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
    37261 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
    37321 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
    37381 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
    37441 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
    37501 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
    37561 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
    37621 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
    37681 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
    37741 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
    37801 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
    37861 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
    37921 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
    37981 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
    38041 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
    38101 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
    38161 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
    38221 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
    38281 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
    38341 ggaaagatgc ccttaataat cgggaccatc gatattctag ttggcgtctt tttcttattc
    38401 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
    38461 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
    38521 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
    38581 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
    38641 acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg
    38701 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
    38761 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
    38821 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
    38881 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
    38941 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
    39001 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
    39061 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
    39121 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
    39181 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
    39241 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
    39301 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
    39361 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
    39421 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
    39481 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
    39541 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
    39601 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
    39661 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
    39721 gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg
    39781 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
    39841 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
    39901 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
    39961 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
    40021 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
    40081 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
    40141 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
    40201 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
    40261 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
    40321 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
    40381 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
    40441 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
    40501 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
    40561 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
    40621 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
    40681 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
    40741 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
    40801 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
    40861 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
    40921 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
    40981 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
    41041 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
    41101 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
    41161 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
    41221 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
    41281 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
    41341 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
    41401 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
    41461 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
    41521 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaatatat
    41581 atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta gtaagttata
    41641 tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg tatttccttt
    41701 gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga ccgcaaacca
    41761 taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg caattagcac
    41821 taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa accctcctct
    41881 tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac ctccaactga
    41941 agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct ctggtttatc
    42001 atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct tgaagcattt
    42061 acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca tgaacctaac
    42121 ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta tattgtcagt
    42181 tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt tgtacataag
    42241 ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc gctttttccg
    42301 tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat gacttatggt
    42361 tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact tgtcaaagca
    42421 aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa atcgtatcta
    42481 tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc agtagccgtg
    42541 ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac catcgaatca
    42601 caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa aaaataaata
    42661 aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg atcaaaaaag
    42721 aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg atcaaggtca
    42781 tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag caataaaaaa
    42841 ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt ttacagcagt
    42901 atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga aaagtgtatc
    42961 gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa aaataaaaaa
    43021 ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag aaatgaataa
    43081 atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa aaaataacat
    43141 atctcatttt acacttttgt tattttatac ttaaaaaata acgacacaga aaataccgtg
    43201 tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa tgccttcaaa
    43261 aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta aataaggaga
    43321 aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag aaaaataata
    43381 tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata ttgactagga
    43441 agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta aataagggga
    43501 atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag tagattagag
    43561 atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag atacacattg
    43621 gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga cagttcgggt
    43681 caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa taatggctag
    43741 acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc ataaacctat
    43801 cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa taccgaatag
    43861 gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt ttttagaaaa
    43921 agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga caatcaagtt
    43981 ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa aagggacgag
    44041 attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata gcttgaataa
    44101 aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata cataccgcat
    44161 agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc ataaaaaagt
    44221 atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt tttgttggga
    44281 atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc tatctttttt
    44341 ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt gtttactttt
    44401 tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg ctgacaaacg
    44461 ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga gggatagatt
    44521 gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta tcatgaatct
    44581 gatgctactt atctttattg tattatggtt aggttacata aatcatgatt ttttgatttc
    44641 aagtttttca tcattaggga tctttacttt tttctattat caaaacatac cgatgaaata
    44701 tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg ttttggggat
    44761 cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg ctttttccag
    44821 cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt ttgcgatgct
    44881 ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg tctcacttta
    44941 tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat tactggattc
    45001 acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg aagagccttt
    45061 agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt atgtcagtca
    45121 cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga ttttgctagc
    45181 agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat cgatgggtgg
    45241 gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga ccatttttct
    45301 gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg cagttgctaa
    45361 tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag catacttgat
    45421 ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg gtttaggtgt
    45481 tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc aaaaattaga
    45541 atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc acaacctcta
    45601 attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca aaaatgtgga
    45661 aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt atgatggtaa
    45721 ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag atattctttc
    45781 ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta taccatatgg
    45841 tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg ggaaatttga
    45901 tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt cccaaagtcg
    45961 ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt gagcaatata
    46021 gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata gacaaagtct
    46081 atgaagagga accttttgca gcattagcta tttatctata tgcagcttct caaagttgga
    46141 aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca atgaatgctt
    46201 ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt gaccgtggtt
    46261 tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg gagtacgaaa
    46321 tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag tgcctttctc
    46381 ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt tacgaaagtg
    46441 ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg tacacagata
    46501 cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa atctgtcttt
    46561 ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga gtcccttgga
    46621 tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt ttacagcgac
    46681 aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt attcttactt
    46741 cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa agaacacttt
    46801 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag
    46861 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt
    46921 aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta cgttcaatag
    46981 gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt ttttaggtag
    47041 ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct agtctgatcg
    47101 actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac acaaatcgta
    47161 attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg ggtcgtccat
    47221 atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa tatcaaaaca
    47281 cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa aaggtaagaa
    47341 gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt caataatcgg
    47401 tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac atatgataaa
    47461 ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat atagttaact
    47521 acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt ttcaattccc
    47581 tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca cctttgttcg
    47641 tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag gttttgcgaa
    47701 atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa ccattatcgt
    47761 ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc catttctttt
    47821 cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta actttaaaaa
    47881 gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta gaataacatc
    47941 aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt ggaattttag
    48001 atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca agtatcttta
    48061 ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt tgtagtcagt
    48121 cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg attggaagat
    48181 aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt tattttccct
    48241 ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga acatgataac
    48301 ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc ggagactcca
    48361 ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta tatacagtac
    48421 gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt agagctaaca
    48481 ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag tgacgagact
    48541 gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat attgtggatg
    48601 tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta gatgtagcac
    48661 aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa gggaaaagaa
    48721 aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg gaaagaattc
    48781 aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga aggatcgata
    48841 gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt agaagagttt
    48901 ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt ttgacacata
    48961 ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa tgcagtataa
    49021 agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct acacacttct
    49081 atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta gcttattgat
    49141 tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa cactcagaga
    49201 acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc tggttgggaa
    49261 aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga gtatgccgat
    49321 aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca atcccagtca
    49381 ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc ttcagggatt
    49441 agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa aattgtacgc
    49501 atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt taatgatact
    49561 tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga aacgtttatt
    49621 tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg tggagaggaa
    49681 ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca aaaaagtact
    49741 cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt taatgttaat
    49801 tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa gatacactgc
    49861 tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc agttttttat
    49921 aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg tttaagaatt
    49981 aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga tggttccatc
    50041 tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat caattgcaat
    50101 aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat cttcgataac
    50161 aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa tatccggttg
    50221 cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa accagcgggc
    50281 taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg tggcaatcgt
    50341 taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt ttgtcaactg
    50401 aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt cttctgacaa
    50461 tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa tctccgtatt
    50521 cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt ttgtatgatc
    50581 atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca ttgttccgtt
    50641 gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc ggttgaatca
    50701 gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga gatctggcga
    50761 tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc atctcgattg
    50821 aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc gatctgctgt
    50881 gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc atttgaactg
    50941 ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg tccgacaaat
    51001 gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc atctgcttga
    51061 gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt gttcagtaaa
    51121 tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc tccctcgtga
    51181 atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag agtggctttt
    51241 tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc tccatggaca
    51301 cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt catatcagaa
    51361 acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt gaccatttct
    51421 acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt aaggataatc
    51481 tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt tagggtggtt
    51541 tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt atttgtgttt
    51601 gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa agagcgaaaa
    51661 ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt ttatttttgg
    51721 cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca aaggcgctgt
    51781 tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg caagtgattt
    51841 ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa atggtctaag
    51901 attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa atccagaagg
    51961 aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa cgccgtttgt
    52021 gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt ttttgaatcg
    52081 agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat tcaaagaagt
    52141 caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat atttgttagg
    52201 tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc ttcataatat
    52261 gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc cgggtgaaat
    52321 tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg aagataagca
    52381 tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta cctatttagg
    52441 ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa atcaggccag
    52501 ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc gtaatgacta
    52561 cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag aaaatgcaat
    52621 ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg gaatcggaga
    52681 acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt atccagaagg
    52741 tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca aaatctatat
    52801 cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta tggatcagcc
    52861 gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa cagccggggt
    52921 aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga caaatgggta
    52981 taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt acccaaaaga
    53041 atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat aagtgataat
    53101 ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga tcgttgcata
    53161 ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac aaggaaactt
    53221 tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg cacataattc
    53281 gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg gattcatttt
    53341 tatccatggg caggatcatt taatgaccac tttattgctg agagatctga ttcaagactt
    53401 tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg gaatcattaa
    53461 acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt atttaggagc
    53521 cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca ttttcatttt
    53581 gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct ccacatggct
    53641 atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag caaccactgc
    53701 tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa tcaacaccac
    53761 ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc cgattgaagg
    53821 tggattttca acggaatata tggggacaaa gggactactg gcttcctttg tcgctgcatt
    53881 tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta aaatgcctaa
    53941 agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct ctttttcagt
    54001 atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct catttgcaga
    54061 agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact taggaatcgc
    54121 tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac cgtcgatcgt
    54181 ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc aattgttcaa
    54241 ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg taggaactat
    54301 gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg ctaagtctaa
    54361 gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg tcaacgaacc
    54421 attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat ttttgttaac
    54481 acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca tgaacagctt
    54541 tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag gaacgggcat
    54601 gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc tgatctatct
    54661 accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa aagcagaggt
    54721 gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg tggcagcgag
    54781 tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg ctggcgcagg
    54841 gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga aagcaccgat
    54901 cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt ttgatatggt
    54961 ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca ccgatcgctt
    55021 aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga gagatcctga
    55081 aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata atgaaaatgg
    55141 gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg ggggctgttt
    55201 caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt tcgatcctgt
    55261 cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa gatgccttag
    55321 agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag gatccttata
    55381 atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg attgaccttg
    55441 caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac agtgattttt
    55501 gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc tttgaagaaa
    55561 tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag cagctggacg
    55621 tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc tggccttatg
    55681 gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt ttgaagtctg
    55741 gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc cacttagctg
    55801 atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt cgaggtgtgt
    55861 catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg aatgaacttc
    55921 aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta gttgaaacag
    55981 cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt gaagccgaag
    56041 cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg ctggatcttg
    56101 ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat tgggaacctc
    56161 tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat ctcagtgttc
    56221 aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct ggaaacgcct
    56281 tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt aaaaatgcaa
    56341 gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg tttcaacaag
    56401 gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac tacaaactaa
    56461 ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt ggcataagca
    56521 aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat actaatcagt
    56581 tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa acaagtgaat
    56641 ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa attggggttt
    56701 atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg gtgaaacaat
    56761 agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc gttagcgtag
    56821 aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac gatttccagt
    56881 catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt atgctaaaga
    56941 gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc agataacata
    57001 aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt gagcacttag
    57061 atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt tttatgataa
    57121 gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc ttatttttaa
    57181 tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc ccctccatat
    57241 tatgttggct ctttattccc actccactgt tgctggtggt ttagaagtga tgtcatacac
    57301 gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt gcaagacttc
    57361 ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag cacggatacc
    57421 gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga tccctggaag
    57481 aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt cttcacgcaa
    57541 gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc caagaacacg
    57601 aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag gcatgcctaa
    57661 ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt cgatcaattc
    57721 aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg tctgtgctgt
    57781 ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga aagagacgcc
    57841 ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat
    57901 gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga agcgatctct
    57961 tgcattgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca ttacttgttc
    58021 cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat
    58081 cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa gaccaagcaa
    58141 gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga taaagttatc
    58201 cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca gcaattcgat
    58261 tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat tacgtgttgt
    58321 gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa agccttcagg
    58381 aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg gtgtttcttt
    58441 aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt cgcggttttt
    58501 agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat agcaaatccc
    58561 taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat tctcatcata
    58621 tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt ttacttcttc
    58681 tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa tacgtcttgt
    58741 gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa cgttcgtcat
    58801 atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct caaaatagtc
    58861 aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata tgattttcag
    58921 ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt
    58981 tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca cgatctccga
    59041 tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt aacgcaccaa
    59101 aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag acagaaaaat
    59161 caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct
    59221 caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg atatcataca
    59281 catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca cggttcaaga
    59341 aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc atgattccac
    59401 gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc
    59461 ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta ctttttccaa
    59521 cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat ttatgaagaa
    59581 ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa atcagatgag
    59641 caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc aaacttttga
    59701 tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat tctcgccatt
    59761 cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc tgcgttctcc
    59821 catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa gagagattgc
    59881 cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt gacttgcgca
    59941 cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg aagacatgac
    60001 aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat gggcttttga
    60061 gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac gtgaaacagt
    60121 ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac ctgatgaagg
    60181 aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg catttgcgac
    60241 acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa
    60301 cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct atgaagcagt
    60361 tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt
    60421 tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta cactgacaat
    60481 tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg
    60541 taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata
    60601 actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg
    60661 tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa
    60721 gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt
    60781 atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt
    60841 tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag
    60901 ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa
    60961 ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag
    61021 tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca
    61081 atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca
    61141 aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca
    61201 attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag
    61261 tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg
    61321 gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg
    61381 gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa
    61441 atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac
    61501 gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg
    61561 ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac
    61621 ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg
    61681 cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat
    61741 taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata
    61801 ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc
    61861 cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg
    61921 gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt
    61981 atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc
    62041 gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga
    62101 tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa
    62161 gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa
    62221 tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag
    62281 ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac
    62341 attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg
    62401 cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat
    62461 cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg
    62521 tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc
    62581 gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg
    62641 agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga
    62701 agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac
    62761 agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga
    62821 agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg
    62881 ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc
    62941 aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat
    63001 gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc
    63061 acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa
    63121 tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat
    63181 tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga
    63241 gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg aattattata
    63301 taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac
    63361 agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact
    63421 aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca
    63481 aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt
    63541 tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt
    63601 tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa
    63661 cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca
    63721 acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa
    63781 agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc
    63841 aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa
    63901 ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc
    63961 cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat
    64021 cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt
    64081 tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg
    64141 ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa
    64201 tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt
    64261 gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta
    64321 gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg
    64381 gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag
    64441 gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg
    64501 ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa
    64561 tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag
    64621 gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa
    64681 caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag
    64741 cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct
    64801 tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga
    64861 atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac
    64921 aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag
    64981 ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag
    65041 cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa
    65101 tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa
    65161 aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt
    65221 caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta
    65281 caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt
    65341 gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa
    65401 agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg
    65461 cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag
    65521 aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg
    65581 aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact
    65641 aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac
    65701 aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa caactccctg
    65761 aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc
    65821 cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa
    65881 atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa
    65941 gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg
    66001 atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa
    66061 gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag
    66121 cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct
    66181 agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa
    66241 cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa
    66301 caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag
    66361 ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta
    66421 gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta
    66481 gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac
    66541 gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg
    66601 aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa
    66661 aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt
    66721 ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg
    66781 cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc
    66841 cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta
    66901 gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac
    66961 agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa
    67021 gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat
    67081 cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca
    67141 aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa
    67201 caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat
    67261 gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa
    67321 gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga
    67381 aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt
    67441 attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc
    67501 ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca
    67561 aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt
    67621 ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg
    67681 gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct
    67741 tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt
    67801 acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt
    67861 attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt
    67921 ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc
    67981 ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt
    68041 ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc
    68101 ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat
    68161 ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt
    68221 ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg
    68281 ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg
    68341 tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc
    68401 tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt
    68461 ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa
    68521 ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct
    68581 acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga
    68641 agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc
    68701 gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta
    68761 gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg
    68821 cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt
    68881 ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg
    68941 tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat
    69001 acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc
    69061 aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca
    69121 ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc
    69181 ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc
    69241 gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg
    69301 gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa
    69361 ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca
    69421 atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac
    69481 tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt
    69541 cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt
    69601 ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg
    69661 ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga
    69721 acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat
    69781 cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata
    69841 cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc
    69901 ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg
    69961 tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga
    70021 aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg
    70081 aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa
    70141 aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag
    70201 acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc
    70261 ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt
    70321 tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc
    70381 agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt
    70441 ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat
    70501 ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta
    70561 cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac
    70621 cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt
    70681 cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa
    70741 gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag
    70801 cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt
    70861 tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat
    70921 gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa
    70981 gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta
    71041 ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg
    71101 ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg
    71161 agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc
    71221 gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta
    71281 tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata
    71341 tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat
    71401 aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg
    71461 attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct
    71521 gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt
    71581 taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag
    71641 cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat
    71701 atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag
    71761 gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc
    71821 catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc
    71881 gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac
    71941 aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc
    72001 caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata
    72061 cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga
    72121 cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt
    72181 acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga
    72241 tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag
    72301 taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag
    72361 taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat
    72421 tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc gtttaactct
    72481 gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt
    72541 gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg
    72601 tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac tgttaagtca
    72661 tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat gatgtcttcg
    72721 tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg tgcagctact
    72781 aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa tggcaaagcg
    72841 cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc taacattgcg
    72901 taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg aggaagagta
    72961 tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt
    73021 ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga tttgatttgt
    73081 ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat tgcacgagtt
    73141 aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt gcgtccgcct
    73201 tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc agtaaattgg
    73261 ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg catgtttagc
    73321 tctcctttta agataggttt atatttctga aattttcagt tctgttttat tgtacaattt
    73381 tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca agtagagaat
    73441 ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat gcataattgc
    73501 tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga aacaccgcat
    73561 tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg gatttttttg
    73621 atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg agcttcatct
    73681 aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag gttttccatt
    73741 aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag actaatcaag
    73801 tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt agatttgcct
    73861 aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc gatcactgac
    73921 tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg gaaaatttct
    73981 tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc atttatggtt
    74041 gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt ttctaactgg
    74101 atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc gcaaattttc
    74161 cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg gattgcgtca
    74221 agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt taggaaaagc
    74281 ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc tggctgttcg
    74341 atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa gacttctccg
    74401 ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg aagtagctgt
    74461 tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt atcgactaat
    74521 ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat tttctcgaaa
    74581 agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc atcagcaccg
    74641 tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat tcctaatttt
    74701 tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa accttcttca
    74761 cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt cccgagacct
    74821 acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg ttttaaattc
    74881 atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat tttagcgtga
    74941 tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa aaaaaattgc
    75001 atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt tatctacagg
    75061 tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga tgcagacatt
    75121 gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag acgcaaatcg
    75181 tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat
    75241 caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc tttaagaaaa
    75301 ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga
    75361 cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg tcaaacttaa
    75421 ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac agtgttgtat
    75481 tattacgcgg tggtcgtgta agagacttac caggggtacg ttaccatatc gttcgcggtg
    75541 ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac ggaactaaaa
    75601 aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta tatagaagta
    75661 attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag gtcctgttgc
    75721 aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc gcttgatcaa
    75781 ccgtgtaatg gttgacggaa aacgcgggat ttcagctaac attatctata atgcctttga
    75841 cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag caatgaaaaa
    75901 cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact accaagtacc
    75961 agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag taaactacgc
    76021 tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca tggatgctgc
    76081 aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa
    76141 ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct tttataagtc
    76201 gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag aaacaaggag
    76261 aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg cccacatcga
    76321 tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa tccataaaat
    76381 tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac aagaacgtgg
    76441 tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa tcaacatcat
    76501 cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc gtgtacttga
    76561 tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg aaacagtttg
    76621 gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat
    76681 cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag caaacgctca
    76741 cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg accttgtaac
    76801 aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa cagaaattcc
    76861 tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga
    76921 aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg aagctgagtt
    76981 aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac tatgtggttc
    77041 agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt acttgccatc
    77101 accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag aagtagaacg
    77161 tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga ctgacccatt
    77221 cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt
    77281 acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa
    77341 ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg ttggtttgaa
    77401 agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc ttgaatccat
    77461 ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga
    77521 taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc gtgctgaaac
    77581 aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact tagatatcat
    77641 cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc
    77701 ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac gtcagtctgg
    77761 tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag aaggtgctgg
    77821 tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt
    77881 tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc cattagtaga
    77941 tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg aaactgcctt
    78001 ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc cagtaatcct
    78061 tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg atgttatggg
    78121 tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg gcggccaaca
    78181 agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa ctcttcgttc
    78241 tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag atgtaccaaa
    78301 atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat ttcttgaaac
    78361 cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag tttcgtgtaa
    78421 aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata cctatcagac
    78481 aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag aaaaatttga
    78541 ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg gtaaaactac
    78601 attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag ctatggctta
    78661 cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct caacagctca
    78721 cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag gacacgcgga
    78781 ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct tggtagtttc
    78841 tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg
    78901 tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg aagaattact
    78961 agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc ctggtgacga
    79021 tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt catacgaaga
    79081 aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag aacgtgacaa
    79141 cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac gtggtactgt
    79201 tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg aagttgttgg
    79261 tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt
    79321 agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg cacgtgaaga
    79381 catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata caaaattctc
    79441 tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat tcttcactaa
    79501 ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg aattaccaga
    79561 aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat taatccaccc
    79621 aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta cagttggtgc
    79681 cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa gcaacattta
    79741 ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct tttttattta
    79801 tt
//
LOCUS       DACDIP010000006        77915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDIP010000006 DACDIP010000000
VERSION     DACDIP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.006"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1329
                     /locus_tag="IV257_000463"
     CDS             <1..1329
                     /locus_tag="IV257_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8813619.1"
                     /translation="SISVIYNIFFEGNDLQVFLPLPFGQEQVFSAKILVVALTIIPFV
                     FPLLVMFILTGWRADLGILLTIIISILLFSLFLIILFCLASLIVFGLARTAVFKKHRK
                     TVSSILLGSSMLIAFAGIMWMNHQTGTIENELTDRHPISILLPFYDAASQPLSQQGLL
                     GWGILAITALGFFLAVKQLIVPKLTEQLTDSNSEFKIKRTHKKNQNVHQLLFSYNAQL
                     VKEPNLIMQVLSSSLLTPIIFILAFALGGEIKLTDLDNKLIGVVFLVGIALAFLTVNQ
                     TSFISNLISLDQENFLFIRSLPISMNQYLKEKFRFGWILQSFLTGGIALLSGLSFQLP
                     LFFIPSLLFGSLFGCYLLSLRYFARDYRLLLLNWTNINQLFNRGSGSLGLVATMMGSL
                     IISIILIVLYGMLALFYPFWLVNIPVIGLVLILSALWIVYYQRTFWKKFE"
     gene            complement(1580..2059)
                     /locus_tag="IV257_000464"
     CDS             complement(1580..2059)
                     /locus_tag="IV257_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8813620.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(2075..2827)
                     /locus_tag="IV257_000465"
     CDS             complement(2075..2827)
                     /locus_tag="IV257_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8813621.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            2944..3014
                     /locus_tag="IV257_000466"
     tRNA            2944..3014
                     /locus_tag="IV257_000466"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2976..2978,aa:Gly,seq:ccc)
     gene            3375..4100
                     /locus_tag="IV257_000467"
     CDS             3375..4100
                     /locus_tag="IV257_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8813622.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            4355..4621
                     /locus_tag="IV257_000468"
     CDS             4355..4621
                     /locus_tag="IV257_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8813623.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            4618..5049
                     /gene="ruvX"
                     /locus_tag="IV257_000469"
     CDS             4618..5049
                     /gene="ruvX"
                     /locus_tag="IV257_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8813624.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            5074..5379
                     /locus_tag="IV257_000470"
     CDS             5074..5379
                     /locus_tag="IV257_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8813625.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(5460..6605)
                     /locus_tag="IV257_000471"
     CDS             complement(5460..6605)
                     /locus_tag="IV257_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8813626.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(6666..7313)
                     /locus_tag="IV257_000472"
     CDS             complement(6666..7313)
                     /locus_tag="IV257_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8813627.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            7507..7800
                     /locus_tag="IV257_000473"
     CDS             7507..7800
                     /locus_tag="IV257_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813628.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            7844..8155
                     /locus_tag="IV257_000474"
     CDS             7844..8155
                     /locus_tag="IV257_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813629.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            8152..8776
                     /locus_tag="IV257_000475"
                     /pseudo
     CDS             8152..8776
                     /locus_tag="IV257_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            9076..9438
                     /locus_tag="IV257_000476"
     CDS             9076..9438
                     /locus_tag="IV257_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813630.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            9475..10323
                     /locus_tag="IV257_000477"
     CDS             9475..10323
                     /locus_tag="IV257_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8813631.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            10313..11788
                     /locus_tag="IV257_000478"
     CDS             10313..11788
                     /locus_tag="IV257_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP8813632.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            12054..12461
                     /locus_tag="IV257_000479"
     CDS             12054..12461
                     /locus_tag="IV257_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP8813633.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            12464..12679
                     /locus_tag="IV257_000480"
     CDS             12464..12679
                     /locus_tag="IV257_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813634.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            12683..13063
                     /locus_tag="IV257_000481"
     CDS             12683..13063
                     /locus_tag="IV257_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8813635.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            13197..13352
                     /locus_tag="IV257_000482"
     CDS             13197..13352
                     /locus_tag="IV257_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP8813636.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            13421..13894
                     /locus_tag="IV257_000483"
     CDS             13421..13894
                     /locus_tag="IV257_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8813637.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            13891..15585
                     /locus_tag="IV257_000484"
     CDS             13891..15585
                     /locus_tag="IV257_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8813638.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            15551..15736
                     /locus_tag="IV257_000485"
     CDS             15551..15736
                     /locus_tag="IV257_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813639.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            15740..16915
                     /locus_tag="IV257_000486"
     CDS             15740..16915
                     /locus_tag="IV257_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8813640.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            16908..18431
                     /locus_tag="IV257_000487"
     CDS             16908..18431
                     /locus_tag="IV257_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8813641.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            18487..18771
                     /locus_tag="IV257_000488"
     CDS             18487..18771
                     /locus_tag="IV257_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8813642.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            18758..19093
                     /locus_tag="IV257_000489"
     CDS             18758..19093
                     /locus_tag="IV257_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8813643.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            19388..20014
                     /locus_tag="IV257_000490"
     CDS             19388..20014
                     /locus_tag="IV257_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813644.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            20756..21592
                     /locus_tag="IV257_000491"
     CDS             20756..21592
                     /locus_tag="IV257_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8813645.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            21657..22091
                     /locus_tag="IV257_000492"
     CDS             21657..22091
                     /locus_tag="IV257_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8813646.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(22159..23481)
                     /locus_tag="IV257_000493"
     CDS             complement(22159..23481)
                     /locus_tag="IV257_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8813647.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(23635..25275)
                     /locus_tag="IV257_000494"
     CDS             complement(23635..25275)
                     /locus_tag="IV257_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8813648.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            25605..26075
                     /locus_tag="IV257_000495"
     CDS             25605..26075
                     /locus_tag="IV257_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8813649.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            26704..27807
                     /locus_tag="IV257_000496"
     CDS             26704..27807
                     /locus_tag="IV257_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8813650.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTTPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            28091..28771
                     /locus_tag="IV257_000497"
     CDS             28091..28771
                     /locus_tag="IV257_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8813651.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            28861..29895
                     /locus_tag="IV257_000498"
     CDS             28861..29895
                     /locus_tag="IV257_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8813652.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            29909..30244
                     /locus_tag="IV257_000499"
     CDS             29909..30244
                     /locus_tag="IV257_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813653.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            30228..32708
                     /locus_tag="IV257_000500"
     CDS             30228..32708
                     /locus_tag="IV257_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813654.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            32764..34323
                     /locus_tag="IV257_000501"
     CDS             32764..34323
                     /locus_tag="IV257_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8813655.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            34320..35684
                     /locus_tag="IV257_000502"
     CDS             34320..35684
                     /locus_tag="IV257_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8813656.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            35855..36925
                     /locus_tag="IV257_000503"
     CDS             35855..36925
                     /locus_tag="IV257_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813657.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            36940..37608
                     /locus_tag="IV257_000504"
     CDS             36940..37608
                     /locus_tag="IV257_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813658.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            37628..38206
                     /locus_tag="IV257_000505"
     CDS             37628..38206
                     /locus_tag="IV257_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8813659.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            38609..39520
                     /locus_tag="IV257_000506"
     CDS             38609..39520
                     /locus_tag="IV257_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8813660.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(39776..40693)
                     /locus_tag="IV257_000507"
     CDS             complement(39776..40693)
                     /locus_tag="IV257_000507"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8813661.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            40895..41332
                     /gene="zapA"
                     /locus_tag="IV257_000508"
     CDS             40895..41332
                     /gene="zapA"
                     /locus_tag="IV257_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8813662.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            41390..41938
                     /locus_tag="IV257_000509"
     CDS             41390..41938
                     /locus_tag="IV257_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8813663.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            42053..44413
                     /locus_tag="IV257_000510"
     CDS             42053..44413
                     /locus_tag="IV257_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8813664.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            44500..44814
                     /gene="trxA"
                     /locus_tag="IV257_000511"
     CDS             44500..44814
                     /gene="trxA"
                     /locus_tag="IV257_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8813665.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            44996..46774
                     /gene="uvrC"
                     /locus_tag="IV257_000512"
     CDS             44996..46774
                     /gene="uvrC"
                     /locus_tag="IV257_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8813666.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            46919..47818
                     /locus_tag="IV257_000513"
     CDS             46919..47818
                     /locus_tag="IV257_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813667.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            47811..49073
                     /locus_tag="IV257_000514"
     CDS             47811..49073
                     /locus_tag="IV257_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813668.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            49236..51134
                     /locus_tag="IV257_000515"
     CDS             49236..51134
                     /locus_tag="IV257_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8813669.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(51433..52152)
                     /locus_tag="IV257_000516"
     CDS             complement(51433..52152)
                     /locus_tag="IV257_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8813670.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            52489..52821
                     /locus_tag="IV257_000517"
     CDS             52489..52821
                     /locus_tag="IV257_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813671.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            52808..54811
                     /locus_tag="IV257_000518"
     CDS             52808..54811
                     /locus_tag="IV257_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8813672.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            54830..55300
                     /locus_tag="IV257_000519"
     CDS             54830..55300
                     /locus_tag="IV257_000519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8813673.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            55321..55905
                     /locus_tag="IV257_000520"
     CDS             55321..55905
                     /locus_tag="IV257_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8813674.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            55920..56906
                     /locus_tag="IV257_000521"
     CDS             55920..56906
                     /locus_tag="IV257_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8813675.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            56896..57207
                     /locus_tag="IV257_000522"
     CDS             56896..57207
                     /locus_tag="IV257_000522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8813676.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            57229..59010
                     /locus_tag="IV257_000523"
     CDS             57229..59010
                     /locus_tag="IV257_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8813677.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            59003..60379
                     /locus_tag="IV257_000524"
     CDS             59003..60379
                     /locus_tag="IV257_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8813678.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            60386..61021
                     /locus_tag="IV257_000525"
     CDS             60386..61021
                     /locus_tag="IV257_000525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8813679.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            61051..62406
                     /locus_tag="IV257_000526"
     CDS             61051..62406
                     /locus_tag="IV257_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8813680.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(62598..63992)
                     /locus_tag="IV257_000527"
     CDS             complement(62598..63992)
                     /locus_tag="IV257_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8813681.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            64084..65055
                     /locus_tag="IV257_000528"
     CDS             64084..65055
                     /locus_tag="IV257_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8813682.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            65052..65834
                     /locus_tag="IV257_000529"
     CDS             65052..65834
                     /locus_tag="IV257_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8813683.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(66003..66374)
                     /locus_tag="IV257_000530"
     CDS             complement(66003..66374)
                     /locus_tag="IV257_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8813684.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            66508..66951
                     /locus_tag="IV257_000531"
     CDS             66508..66951
                     /locus_tag="IV257_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8813685.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(66994..67803)
                     /gene="yidA"
                     /locus_tag="IV257_000532"
     CDS             complement(66994..67803)
                     /gene="yidA"
                     /locus_tag="IV257_000532"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8813686.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(67822..69189)
                     /locus_tag="IV257_000533"
     CDS             complement(67822..69189)
                     /locus_tag="IV257_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8813687.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(69173..70003)
                     /locus_tag="IV257_000534"
     CDS             complement(69173..70003)
                     /locus_tag="IV257_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8813688.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            70166..70603
                     /locus_tag="IV257_000535"
     CDS             70166..70603
                     /locus_tag="IV257_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8813689.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            70679..71299
                     /locus_tag="IV257_000536"
     CDS             70679..71299
                     /locus_tag="IV257_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8813690.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            71488..71994
                     /locus_tag="IV257_000537"
     CDS             71488..71994
                     /locus_tag="IV257_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8813691.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            72011..72910
                     /locus_tag="IV257_000538"
     CDS             72011..72910
                     /locus_tag="IV257_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8813692.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            72997..73881
                     /locus_tag="IV257_000539"
     CDS             72997..73881
                     /locus_tag="IV257_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8813693.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(74073..74144)
                     /locus_tag="IV257_000540"
     tRNA            complement(74073..74144)
                     /locus_tag="IV257_000540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74109..74111),aa:Arg,seq:ccg)
     gene            74204..75538
                     /gene="rpoN"
                     /locus_tag="IV257_000541"
     CDS             74204..75538
                     /gene="rpoN"
                     /locus_tag="IV257_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8813694.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            75741..76778
                     /locus_tag="IV257_000542"
     CDS             75741..76778
                     /locus_tag="IV257_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8813695.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            76819..77820
                     /gene="gap"
                     /locus_tag="IV257_000543"
     CDS             76819..77820
                     /gene="gap"
                     /locus_tag="IV257_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8813696.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT        25872 a        13373 c        16251 g        22419 t
ORIGIN      
        1 agtatatcag tcatttataa tatctttttt gaaggaaatg acctgcaagt atttttgcct
       61 ctgccttttg gacaagaaca ggttttttca gcaaagattt tggtagttgc tttgaccatc
      121 attccttttg tttttccgct tctagtcatg tttattttaa ccggatggcg agctgatcta
      181 gggattttgc ttactatcat tatctcaatc cttttgtttt ctctattttt gattattctt
      241 ttttgtctgg ctagtttgat tgtttttggt ttggctcgaa cagcagtgtt taaaaaacat
      301 agaaaaacag tttctagtat cttgcttggc agctcgatgc tgatcgcatt tgctggaatc
      361 atgtggatga atcatcaaac ggggactatt gaaaatgagt taacagatcg gcatccgatt
      421 tcgatattat tgccattcta tgatgctgcc agtcagccac tttctcaaca aggtttattg
      481 ggttggggaa tactagctat cacggctctg ggatttttcc ttgctgtaaa acagctgata
      541 gtaccaaagc ttacagagca actgactgat tcaaacagtg agttcaaaat aaaaagaacc
      601 cataaaaaga accaaaatgt gcatcagctt ttatttagtt acaatgctca attagtaaaa
      661 gaacctaatt tgattatgca agttctttct tcttccttgt taactccaat catttttatt
      721 cttgcttttg ccctaggagg cgaaataaaa ttaactgatt tagataataa gttaatcggt
      781 gttgttttcc tggtagggat cgcattggct ttcttaacag ttaatcagac ttcttttatc
      841 agcaatctta tttctttgga tcaagaaaat tttctgttta tccgttcttt gccaatctct
      901 atgaaccaat atttaaaaga aaagttcaga ttcggatgga tattgcagag ttttttaact
      961 ggtgggatcg cattattaag cggactgtct tttcagctgc ctttgttttt tatccctagc
     1021 ttgctatttg gttctttatt cggctgctac ttattgtcac tacgttattt cgctagagat
     1081 tatcggttgc tgctgctgaa ctggaccaat attaaccaat tatttaacag aggttcagga
     1141 agtttgggtt tagtcgcaac tatgatgggt tcattgatca tcagtattat tcttatcgtc
     1201 ttatacggaa tgttggcttt gttttatcca ttttggttag tgaatattcc tgtgatcggt
     1261 ttagttctaa tactttccgc tctatggatc gtttattatc aacgaacgtt ttggaaaaaa
     1321 tttgaataaa gaagaggggc cgtgacaaaa atcatgtcgc agcccctttt ttacagatag
     1381 agggtgttca aaagtcagtt tctcagtatt aagtcaattt tatgtaaaaa tgtgagaagc
     1441 tatttttaca aaactttttt taatactcat aattacttct tattgctcct gcggttactt
     1501 gtcgcaatgt ttgtgagata ttttgtcatc gctcaaggct gaacgtctct ttcacaacct
     1561 tttctttgtt acacttcgtt tatttaaaaa gccgtttttt tcgtttcatc atgattgcgt
     1621 cacgctttac tttgaaatta cggatgttat ggttaaagac ttctgataat acaagaccta
     1681 aagcgattgc tccagcaatc ataattgctt ggaccgcata atgagccgct tctcgatagg
     1741 attcagtaac gagatttcga accgcttgat aagctaaacc accagggact agaggaacca
     1801 tcccgggaat attgaaaatc gttacgggca ttttcaaacg tttagagaaa agatcgctga
     1861 tgaatgctac tcctaaagaa ccaagcaatg aaccaaaagc agcagatccg cctatttgaa
     1921 cacagatcca gtagatcatc cagccaaaag caccagaaag accacaacaa atcaatgaac
     1981 gcctaggtac atttgtaata acggcaaatg ccgcagtcgc taaaaaacta aaagaaaact
     2041 gaataaatat cgtccccata cttgtgttcc tttcctaata aaaaagctgg aaaataaaag
     2101 caatcgcaaa accgatcatc gtagcagtca tcatcgcttc tgttccgcga gaaactcctg
     2161 agacataatg tccagctagc aaatcacgga tagcgttcgt aatttgaact ccaggaacta
     2221 aaggcatcac acatccgata atgattagat cctgattgct tcctaagcca aaacgtacgc
     2281 tgtagatagc ggctatgcca atcaataaag aagataaaaa ttccgataaa aatttgattc
     2341 taaaaaattt caaactatat agataagctg cgtatcccaa tcctccaatg actaaagtca
     2401 caggtaaatc ggtcatcttt ccgccaaata acaccataat tgttccactg atcactgccg
     2461 cgctaacgat ttgcaaccag acaggaaaga aagaacggtc tcggtcgatt gcttgcagtt
     2521 cgcagtaaag ttcttctaat gttagctctg aagcaacata tttacgtgaa agatcattta
     2581 cttttactac tttttctaaa ttaatcgaac ggttggtaat ttgttccatt cggatggtcg
     2641 aggtacgatc aagaccaata aagagaccgg tttgagtgac atagctgacc aaacggaaat
     2701 ttccagaagc taaagcaatt cgactcatcg tatcttctac ccgatacatc tccgcattgc
     2761 tttccatcat gattttccca gcgagtaaac aagtgtccag caacagatct aattcttttc
     2821 ccgtcatagc atcttctcca ataattgatt gattagtttt attttactac aaaacatacc
     2881 agaagcgtaa agaataaaaa aacttgagga aggaataaaa gaggcgtata ctgtttttgt
     2941 attgcgggtg tagtttagtg gtaaaatccc agcttcccaa gctgatgtcg cgggttcgat
     3001 tcccgtcacc cgcttaaaat aggttgtgaa aaagctgctc tgcaacaagt aataagaaca
     3061 gccaagcaaa aacaacttct tatgttttta acttggatgg acttattacc gcagatgcaa
     3121 gcttttgcgc accgtttacc aagcggttgt gaaatcagtg tattgtcttg agtatgaaga
     3181 aaatttttga ataataataa ataccttaca gatagagtgg tgactgatta gagacgagga
     3241 atccgtctct tttttgtatt tttaagccat cttaaaacag gttttatttt ttgctgtttc
     3301 tatttctttc tagtatttat ttatagggga acatatgtta agatttaggg agctgttagt
     3361 aaaggagtaa agaaatgagc aaaaaaacaa ccaaggcgat cgtggggatc gtcgctgctg
     3421 tgattgcagg ggcattcggt gtttccacca cacaaaaaga ttcagatatc tttcagcaga
     3481 ttacaactat ttttgattcc acaccacaag cttctcaagt agcggatgtg ccagaatatg
     3541 atggaaaaca tcaagaaata gaaatcaatg ggaatcagcc ggaatttaca aaagaggatc
     3601 ttagcctttc caaaggaaca tgggaaagct attcaaatat tgataaattg aacagagtag
     3661 gacaagccaa tgctatgctt ggaaaagatt tgttccctac tgaaaagaga gagcctttgt
     3721 atattgaccc gacaggttgg aaacaaaaga aattaagtga cgggcaatgg ttatataatc
     3781 gcagccattt aattggtttc cagctgactg gtcaaaataa caacattaaa aacttgatga
     3841 cagggacacg ttcgttgaat gctccataca tgcttgctca cgaaaatgat attgcggcat
     3901 atatcaaaga aacaaatcat catgtacgct accgtgtcac tcctcatttt gaggggaatg
     3961 aactggtagc caggggagtg caattggaag cagaaagcat tgaagacaaa aaaattgaat
     4021 tcaatgtctt tatctacaat gttcaagatg gctacacgat caattatgag acaggacaag
     4081 caacaaaaac aaaaaattag tagcagctat tcgaattttt tatgaatatt atgaaaaaat
     4141 tgtgtaaaaa catacattca aagaaatata gcgtatactt tatgaaaaga gtacttttga
     4201 taaagctaca gaaaaaagga aaaaaacata caaatagtgt ttcttaattt attcggaagt
     4261 ttggaatatt tgtttgtagt tttccctttc ttcgagtaaa ataaggttgt agaaggtaag
     4321 ctggaattac cttgcgggaa agaggtgtag aatcatgggt tttacagatg aaaccgtacg
     4381 ttttggtttt gacgatagtc gcaaaaaaga agtgagcgaa acattagcga ttgtttatcg
     4441 ggctttggaa gaaaaaggct acaatccaat caatcagatt gtcggctact tgctttctgg
     4501 agacccggcc tacattcctc gttatcgtga tgctcgaaat ttgattcgcc gtcacgaacg
     4561 cgatgagatc atggaagtaa ttgtcaagga ctacctctca aatcatggag tgaatttatg
     4621 agagtaatgg gcttggatgt cggttctaag acagtaggtg ttgctatcag tgaccccttt
     4681 ggctggacag ctcaaggagt agagatcatt cggatcaatg aagcagaaga agagtttggc
     4741 tttgatcggt tgaaggaatt agtagcacaa tatgaagtag accggtttgt agttggatta
     4801 cctaaaaata tgaacaattc catcggacca agggcagaag cttccatgtc atacggagaa
     4861 aagatcaaag aactattctc cttgccagtt gattatcaag atgaacgatt aaccactgtt
     4921 caggcagaac gtatgctagt agagcaggca aatacttcaa gagccaaacg aaaaaaagtc
     4981 atcgataaat tagcagctgt gatgatctta caaaattatt tagacggctc aggtaagcaa
     5041 ttattttaat aataaaagaa aagaggtaat cttatgacag aacataacca ttcccacgac
     5101 catgaaggac acgaacacat cacattggtg gatgaccaag gaaatgaaac attatatgaa
     5161 atcctactaa cagttgacgg acaagaagaa tttggacgta actatgttct tttatatcct
     5221 gctggtgttt cagaagatga agatgtagaa ttacaagctt atgcatacat cgaaaacgaa
     5281 gatggaacag aaggcgagtt agaacaaatc gaaacagacg cagaatggga catgattgaa
     5341 gaagtattca atacattcat ggcggaagaa gaagaataga ctataaagct aggagaaacc
     5401 ttgatatgat agggtttctc ctttttttat tttttactgt atgccatatt gtatgccatc
     5461 tagtcaatat ttaagtgttg tactaatttt tcaacagtct tttctttcgt ttcttttgcc
     5521 aagtgaccgt aacaatcgat cgtctgacta atttttgtat gccgtaatct agtttgaact
     5581 tctaccatag tagctccagc gtccaagagc attgaagcat gagtgtgtct tgctacatgc
     5641 acaccgagaa atggaaccat agctttttca caaatagtaa tcaatcttgc ataaatagcg
     5701 tggtgtgtga ttagttcttt atagatacca caaaaaatca tagtcggatc agttacacca
     5761 agtgtcagca tatatttccg ctgattaatc tgccattttt ttaatataga aattgttctt
     5821 tcatctagtg gaagaacccc agcactacaa tcagttttag gatcactgat tttatagcca
     5881 tttttggatt ggcttagtgt tttattgatg gtgaccgtat tttcttgaaa atcaatgtct
     5941 gaccaattca atgcaagggc ttcactgatc ctaaccccgc tataaaagag aaagcgtagt
     6001 aacgttttat caaagtcgtt tcgataagtt tcaggtttgc tgtttaggta atcaaacaag
     6061 gtgatcagtt gttctttact gtaaactttg accttttctt tatgcttagt ggtggttctt
     6121 ttaggcatta atacgttgtc tagtggatta gtctcaataa tcccaagatt gatagcgtag
     6181 ctaaaaatac gcttagaagt tgaatgaagt accttgtatt ggtcagtagt ttcagcccag
     6241 ctatttacaa tttgttgggc tttttttacc gtgacttcat tcacacgaag atttccatat
     6301 tttggaagaa tccactttct aaacttgatt tcagttttca tgaaggtggc ttctcggaca
     6361 gtggttttat atgtttctag ccacaagtca taaacttctt taaacgtttc gttgttcgct
     6421 tttttaatac cgtttttatc gtagtccact tgcaagcgtg acaaggcaag ctgggcttct
     6481 ttttttgttt tgtaacctcg tctacgaacc ttatcaggtt tgccagtcat tttgttcatt
     6541 ccaagatagg cttcatagag ccaaaattta ccttttttag tttcatattg cttgaatgtt
     6601 gccataatag ttttccctcc atagtgtagc aagccgtggg ggcggttgga ggaggcagtt
     6661 tattattatt ttgaatagtg tttatttagc tcagtaacaa gagctgtgaa ctcttttaga
     6721 agatcatttt cataaaattc ttgatactct tttttctcat ctgtattttt ttctgaatca
     6781 ttaattaatg agtcatatcg taaaagcgta tttagaagag ctgcgagata agcaattggg
     6841 gtactcccgt caggaaattc aattcgagat cctgttttaa gatattttat tgcgtttgtt
     6901 aaataaatct tctgtgagac agtcaggtta tcttcttcga tgttattgat agcattagaa
     6961 agattagaat caattataga gttatttttt tcaatagttt ttttcaattg cttttcaagc
     7021 acatttcgct taatttcggg catcatatgc atatctctta atgtataaga tccgtttaat
     7081 agataggtag tggatacatc taataccttt gaaagttgtt taagcctggt gttattggga
     7141 agattatagc cattttccca attactaacg gcacctttac tggccggtgg ggataattta
     7201 cctccaagtt gttccattga gagtcccttt tcaattctag ccagtctaat tctttgtcct
     7261 gtctccactg cttcggggta tttttttcta gccacatttt caccaccatt catatatttt
     7321 ctctactata tcacgattta aatatgtgtg aaacataaaa gttcaaaaaa gtatgttaaa
     7381 attcttgcaa atgaaaaggt atgatggtat attgagttta taagaggtat agaaatgtat
     7441 tcaagagcct cttgcatttt tttaaaatct ttgtatagaa atgtatgcta acgagggggg
     7501 ataaatatgg aattaaaaat tgacaaactc acgcttgatg atccagcttt taaaattatt
     7561 gacgatcgag ttgttcaaca agtagagcaa actcaaggaa cgttactttc attattagaa
     7621 aaaaagtacc agtttccaaa atatatgaac aaggcacaag ctgctaagta tatgaacgtt
     7681 tcatacaata ctatgatgca gaaatatgtt cctaacggtt taaagttgat tatagttgat
     7741 ggagtcgtaa gaatttcaca agatgaatgt gatcgcttta tggaagaata ccaaaaataa
     7801 acgcaagccg tgggggcaaa gggattaata ggaggaaata ataatggaat cgacacaaaa
     7861 taaagcaatc gaaaaagtat tatcaacaat tcttacagaa gacacagcgg cagaattagc
     7921 aaacttggaa ggcaaagcgc tagaagaaac gtttgaatgt ttgtacgaac aaatggacta
     7981 tcaaaaattg ctaccacaag aaccaacggt aagcggagta ttgcgtggct tgaacgattt
     8041 agtacaagca gagtttaaag aacgactttc tattgaagag tatcaagaaa ttttgtatca
     8101 gcaagttgag ttgttagcga gcttgctagg gcttgaactg gaggacgttg aatgaaaact
     8161 gaaatttgga acggacacat tataagattt gtcgatatta atgatgaatg gtgggcagtg
     8221 gcgaaagatg ttgcggaagc gttaggtctt aaacaggtta ctagagccat tcactctttg
     8281 cctaaagatg gggttactac tagtaaggtc atcgattcat tgggcagaac acaagatgta
     8341 aacatcatca acgaaaaaaa atatttaccg catggcattc aaaagccgta aaaaagaagc
     8401 cgaggcattt caagactgga ttttcgacat catcaaagaa ctacgccagt ccacaggact
     8461 tgaaggcttt caagtattcc gcatgctaga caaagaacac caaaaagaag caatgactaa
     8521 attaagccac gctattactg aaccaaaacc agtagactac attaaagcga atgtgatagc
     8581 caataaagct gtatcaacta tttatggtca ttccaaaatg gtaaaaaaga aagatatgac
     8641 tcctgaaatg ttggttgatc gtgaacctat tttagatgag acagtagaat taatgactgt
     8701 taaagaaaaa tatgggttgc agttttcagt gagtgagaaa atttataatc gttctgctga
     8761 attgcagaca acttaggagc attaacatga tcgagaacaa agaaaaagcc ttatccataa
     8821 cagtcttggc ggacaggata aaggcaactt aattgggata aaacccgata ttctttgttc
     8881 cgattatacc atattaaaaa atcggaggga accattatga aacgaaactt aattatagga
     8941 agcattttaa tcggcggaat tttcggctta ttaccattaa ttgcaaaaat agctatcttt
     9001 gcaggattag cgttatatct cttcactgct tatgacgagt acgactatca attaagaact
     9061 ggagggcata agtagatgta caaaacaaaa ctattaaacc aactcgatag ccttgaatta
     9121 gaggaaatta atcaagggat cgctgaacta gaaaataata tcgggaaaac ctactttgga
     9181 aattcattca atgaaaaact aacagttttg tatgtactca aaaaacatgc agaacacaaa
     9241 ataatttgtc gagaaatcaa cgagctaaaa aatcagattt taaccgcatg gttaaacatt
     9301 actgatatgc aagaagcaag ggtgaaaaca tttaatacat gggtaaagta tcagaatcaa
     9361 ttaaaaggtg ctgaatttgt tcgggatggg ttgaaatacg aacttgagca gttgaagctc
     9421 atggaggtgt ctgaatgatt tataccagta ttttgaaaaa ggaggagagg tttattggca
     9481 aataattatc aagcagcaac ggaattacaa aagtcgggat ggtcaattta tcccttatct
     9541 cctggaacta aaattccaat aaaaggctct agtggttgga atgatgcaac taacgattct
     9601 aatcaaatta atgaatggtg gacggaagat gcaactagaa atattgggtt aatgcttgaa
     9661 gatcatcaaa tggtggtagt tgacctagat caacattctg aaacacgaaa cggcgttgag
     9721 aattataaaa aacttgcttc aatgtatgat ccatttcctt ctacttatac ggaaatcaca
     9781 ccaggtaaag gaatccattt cttctttaaa attcctgatg gtgtaattat ccaacaagaa
     9841 acaggtgcat ttgctgatat cttcgggcgt gatgataaag gtaaaagttt aagcgggatt
     9901 gatattatca ctaaaggagt tccaatagcg ccaactatga ttatttcaag aagtgttggg
     9961 agagcatatg aacctattga aaacgtatct ctaaataata ttgcaccagc tcccgattgg
    10021 ctagtgaacc tactgctaaa aaaaccgcaa gaaataaaga ctcaataccg acccgtagca
    10081 aaaacaggga cagcaaaaaa attagatatg attgttcaag gcgctggcga aggtagtaga
    10141 aacgatcatt taactaagtt atgcggttgg ttactatggc acggtgtaga taatcaaact
    10201 ttagcagaat tgatttatac agcaaatgcg tacaatgaac caccgctaaa agataaagaa
    10261 gtgaatcaaa ttattcgatc gatgattaaa aaagatttga gggggaaagc tcatggctca
    10321 taagcataaa ttagatagta ttttagactt tcccgaagcc agcgagcgtg aagacaatat
    10381 cattgaattg aaaacctgga tgtcacgatt aagatgcaat aaagacgatc agattaaaag
    10441 taacagtgtt gtgaatgcag aattgatttt aaccaatgat agcaatttag ctggaacaat
    10501 tgcttataac gaatttagcg gatacattca cctattaaaa gattctcctt ggatcaatcg
    10561 taccgctgga gaatgggaag acagttttga ggatgcctta acggcatata ttgaagaaaa
    10621 ctacaatgta gtttttgatg acaacaaaat acacaaagca gtagtaaatg tggctagaaa
    10681 gaatgttttc aacccagtaa aagaacgtat cgaaaaagtt aaatgggatc aacagcctag
    10741 actggaaaca ctgtttattg accttttagg ggtagacgac aacctttaca cacgagaagt
    10801 aacaaaacgt tggatagtcg gatcagtcgc acgtatctat aaaccaggaa taaagtttga
    10861 gatcgttcca gttttggatg gaccgcaagg gattggtaag agtaccgttc cagcattact
    10921 ttacacggat gattttttca ccgattcatt agattcgcta ggtgaaaaga aagatgatta
    10981 tatgcagctt caaggcaatg taattattga acttggagaa ttatcttcaa tgaacaaaac
    11041 taaaattgaa cagataaaaa acttcatcag tgccaagatc gataaaatta gacctcctta
    11101 tggtcggaat gtcatagctt gggcaaggca atgtgtattt attggaacgt cgaatgatgg
    11161 gcagtatttg aaagatgata caggaaaccg ccgtttttat cctttgcctt gtaagaataa
    11221 gccaaagatg aatccgttta aaacaaagga tgaatatttt cttcaagtgt tagctgaagc
    11281 aaaggtgctt tttgataaag gacaacgaat ctattttagt cccgatgaag ataaagaagt
    11341 cttagaaatt gcaaaagact atcaagagga tgccaagatg gagaatccaa ttaaagaagc
    11401 aatcactaaa tatttggaaa tggaaatccc gtataaatgg gaagaagctc cagcgtgggc
    11461 gagacgttct tattatcaga actatcctga cagtaaatgt aatgagaaaa atttacagca
    11521 tttctataat ggagctacta tgcaacaaga gttttattta gttgatagcg tactcaccgc
    11581 tgatattttg gaagctgttt ttgataaaca agcaaaggat ttattgaatg gtcgatcaga
    11641 tgcagaaact aaaaagattg ccttgattat tagcagtatt cctggttggg aacggaagca
    11701 actgccaaaa agaaataaac gacgaggatt ttacaataag tcaaatgcct atgaaaataa
    11761 aaatcgagct ggaaagtaca aaaagtaacg ggtagtaacg ggtcagtaac acctagcccg
    11821 ttactagttt acccttactg ccccaaggga tacagcaatt tggtaacggg gtaacacctt
    11881 atttccttaa aacttttgaa atagttagct aatatgctgg ggaataatgt tactggattt
    11941 tacccgttac ctgttaccaa aacgctagaa cccttgtccc cgttgggatt gagtggtaac
    12001 gggtacctgt tacgtaggtg ttaccacgtg ttacgaatca aggaggaaag taaatgctca
    12061 atattatttc aacaaacaaa gcaccgaact ttcaatatac agatgaaatg gatcggtttc
    12121 taatgaacac gctggcattt agtgtgggat tggtaacaga agattattcc acatttgatc
    12181 cagaagtatt aaagataatg gaagaagaac ctgattggct gcaggagtca gtagcgtggt
    12241 gtcaatcgct agtagtggga tcattggtag atagtggcaa ctatgatgat acaggtgagc
    12301 taatggatga atttaattgt ctgcttaacc tatatgatcg agcgagacaa cgagagctta
    12361 catcgaatga ggataatttg tttttaaaca tccatgataa gttcttggct ttgctactaa
    12421 cagatgatga attgataact aatttattgg aggtagaata attatgcata tggaaagtat
    12481 tagatacttc gatggtgaga aacacattga gtattcaaac actcaagaag atgtagactt
    12541 tattagtttt gaggaagata aaatcactaa agttaatttt aaagacggaa cgtgcttgaa
    12601 gatagtttcg ccttatattg aatacaaatc aaaatgggaa caagatgatt ctgacgaacg
    12661 agatcgcttt ggttggtaat ctatggcagt taaaaaacaa tgtaatcatg ctggatgtaa
    12721 aacattgatt gattataggc agaagtattg tgggaagcat aaagcaaagc aaacggcaat
    12781 taagcgtgaa gaaagaaagc aaagcgaagg taaatatttt caattctatc aaagtagaac
    12841 atggcgaaag gcttcatact tgtatcggtt aaatcatcca gtatgtgagg attgcctaga
    12901 agaaggcttg ataaggaaag ctgatgttgt agatcacaaa attgaactaa aagatgattg
    12961 gaatagacgg ttagatgaaa ctaattttcg ttctttatgc catgcacacc ataattccaa
    13021 gacagccaat gaaagacaaa gacgagaaaa gagtaccctt tgagttaagg gggcatagtg
    13081 tgaaacttac tgacaatcga tgcctactca ttttggtaca aataaccgtt gtgaaaaggc
    13141 ataagggtaa ttacaaataa cgattacact tgtaatcaca agtgaaaaag tatataatga
    13201 aagtaggaga tttattgaaa aatacaattg ttattactga taaaacaggg aaacagcgca
    13261 ccattgagtt acctcatttt ggtcaagtga ccattcaaat gcagaacgga aaaatcattt
    13321 atatagataa attagataaa gaaaaattct gatcgaaaaa cgaaggaatg tgagcttaat
    13381 tgcttacgtt cctttttctt ttgtctgaaa ggaggacaac atgggacacc ctaaaatatt
    13441 acacgataca aaaggcaata tatcaagtga agaaaaagca atccgtgtgg acgctagaga
    13501 agaattgttc aagcagcaac cattaataaa tatcacgccc cctgactgga tggcagcgag
    13561 cgctagaagt gaatggaatc gtatcgtacc aacattaaaa aaagattatc cattgagtga
    13621 agcagattat ggttcattgg tagcctattg tttagccttt gctcgaatga aaacagccga
    13681 agccgagata agaaaatcag gtacgtttat cacatgcgaa aacggagtaa agaaagccaa
    13741 tccagcggtt cgggttcaat cacaagctat gagtgatttg aaaaaacaag ccacctcact
    13801 aggtatgacc ttagaatcac gatcaaaact agctttgaac aaggttaaaa atgatgaacc
    13861 cgaagacccg ttcaaagagt tgatgaattc atgaatgatt acattgagaa agtcctatca
    13921 ggtaagctga ttgcacccaa gaaaattatc caagcgtgtg agcgtcatat aagcgatttg
    13981 gagcgttcta agtcagatag cttcccttat gtgttcgatg aagaacaagc caccaaagcg
    14041 attaaattca ttgagttgct accatctacg gacggtaaag caatcaagat gttaggattt
    14101 caaaaattca ttctaggcag tctttatggc tggcgtacta aagaagggaa ttacaggcga
    14161 ttcaatcgag cgtttaccag tatgagccgt aagaatggga aaacgtatat cgcaagtggc
    14221 atggctgcca atgcgttgat tatggaacaa gaaccagcag aagcaaggca agtattgttt
    14281 gtaagtaacg ctttgaaaca agctaaattg ggctatgata tgctgtctaa ttcacttaga
    14341 aacgtggtca agtctagtaa gtttttaaga ccacaactga aaattatgaa ctctaagatt
    14401 cagcacttgc cctctaattc gttcgctatg gcactggcta gtgaaaccag cacgctagat
    14461 gggtttgcac caacaaccgc aattcttgac gagtggcacg aagcaaaaac tcgtaaaacg
    14521 tacaacgtca ttaagtcagg aatgacccaa caaaagaacg gcttattgtg tgttattagt
    14581 accgctgggc ttgatttaaa cgttcctatg tacgaggaat acttattgtt agatcgtgtg
    14641 ctaaaaggcg aagaacaagc cgacaggtac tttatagcga tatgggaatt ggacgatccc
    14701 gaagaaattc acgatcaaga gaaatggatc aaagccaatc cgatttttga aagtgaagaa
    14761 atcaaaaaag taatgattcc aaccattcaa gatgatgtga accttgcttt gaaacaaaat
    14821 aaccttaatt ctgtattggt gaaaaacttc aatttatgga gacaagcgag tgaggacagc
    14881 tatatgattg ctgaagactg gcaggcaacc gaagtagaac cacaagatat tacaggcaag
    14941 cccgtttata tcggagtgga tttatctaaa acagatgatt taactagcgt ttcatggatc
    15001 gtaccgctag ataacggcga actttattgt gattcacata gctttgtagc caccaaatat
    15061 gggcttcaag acaaagaaaa gcgcgacggt ttgccttatc gagaactaga aaaagctggt
    15121 gagtgttcca ttactcaatt agaaagtgga atcgtggact atgaccaagt atttcagttt
    15181 attcaagatt tgattcaaga aaatgatttg gaatgtatgg ggatttgtta tgacccgtat
    15241 aacgctaatt cgcttatcag taaagctgaa aaagccaact acccaatgtt agaagtaaga
    15301 caaggaacga ttactctaaa cgttccgacc cgaactttta gagaacaagt ttatgaaggc
    15361 aacgttattc acaataaaaa tacgattctc acccatgcag tgaacaacgc tattttaaaa
    15421 acggataaca acggcattca gattaataaa tcaaagaaca gtaacaaaat tgatccaata
    15481 gctgcattaa tcaatgccta tgtgtttgca atggattact tcaccacaac ggaaggagcg
    15541 aaagcagaca atgaatttta tacaagtgaa gaattttctt tctaattaca ttcataccgt
    15601 tcttttactt cttggattgg tgtgtgtgtt ggttgctatc accttactaa caaatgtcta
    15661 ctatggcttg ttagcgctgg gcatagtgct tattggggta gcggtcatgc taaatacaga
    15721 acagaaggga ggttaaaaga tggcattttt taaagcgaga caaaatacaa cgggagatcc
    15781 tttcctacaa catgtggtat caatccaatc ggatgattac accaccagct ttacaagcgt
    15841 tcgtgcatta agaaatagtg atgtgtttgc agccgttcga attattgcta gtgatattgc
    15901 ttcaagtccg attcaattgg tcaaaaacaa tatgccacaa gctgataatg aactggtgaa
    15961 gttactaaac gagaaaccta attcagaaat ggatggttgg catttaaagt ttgctttggc
    16021 ggtcaatatg ttgctaaacg gtaatagctt tgcagagatt aaacgcaacg gtgaaaaggt
    16081 agaagaaatt cacttattac ctaactcaag tgtaacggtt actcaattag ataatggtac
    16141 gttgtcttat cagattggtg ataaaaaaag acgtgtgaag tctagcgata ttttgcactt
    16201 caaatatttc acccaagatg gtttaacagg attaccacca ctttatgcgt tacgtgatga
    16261 actgaaaatt caacaagctg gcaaccgcac attgcacaat ttctttactc gtggtgtcag
    16321 tgggtcaggt attttgaagg ttcataagtc agatttagac ggatcagcaa agaacgcaat
    16381 tcgtgaaaag tttgaagaag ccaacggttc aagtagtgga gataatgctc ttagaacgat
    16441 cattcttgat gaaacaatgg actacaaaac attagaaata aacaccgatg ttttgaagct
    16501 tatcaattcc aatgattgga acacaaaaca aattgccaag gcgtttggtg taccgattga
    16561 gcgtttaggc gttgaaaatg aacattctag caccgttcaa agtaatctcc agtatatcca
    16621 aagtacgctg attcattact ttaatgtgtt tgtgagtgaa ttagatacga aacttgaaac
    16681 caatatccgc tttaattccg atcagttact agagactgat cctgaaacaa aagtaaaaaa
    16741 tgtattggat caggtcaaag ggtcacttct cacgattaat gaggggcggt cgaaaatggg
    16801 gctacccccc atggatggtg gcgatcgttt actagcaagt ttgaacttta cgtatttaga
    16861 tacgttggag aaatatcaat taaaagaaca ggaaggagtt acaccagttg aataatgaag
    16921 aagaaaagga aaaacggctg acagaagaag ctgagctaac agccgattct cccaaagtag
    16981 agaaagaaaa tgaaaaacaa ccaacagacg gcaaaactat ttcaggctat gcgttgaaat
    17041 tcgggcaacc gtcaaaagat ttaggcggct ttgtagaagt gattacaccc gaagcattaa
    17101 aagaggtgga tttatcaaat gtgttcttat tatatggaca cgattattca aagccactag
    17161 ctagtgtcaa agcaggtacg ttgaaattga atgtcgatga tacaggatta cattttgaag
    17221 cagagctaac cgacacgacc tattcaaatg atgtgtatga gaatatctca aaaggggttg
    17281 ttgattctat gtcctttggg ttcgtgcttg gattagattc attcgacaaa aaagaagatg
    17341 gcacaattga acgatcaata gataaaatca aagcacttaa tgaaattagt gtcgtgaccg
    17401 ttcccgctta tgattcatca aatgtccaag taaataagcg ttcttacgaa tcgtttatga
    17461 gtaacaacca agcaaaacaa acaaacaata gcttagaatc cacttctaaa gcacaaaagg
    17521 agagtaataa catggaaaaa actttaatcg ataacgaaaa aacagaattg cgtgggtatg
    17581 aagaatatat ccgttcacaa ggcgaagtgc gtgatggagt cactactgta aatgcagcgg
    17641 cagttgttcc cgaagaagta atcggtgaag tctttgattt gaaacgttca aattataact
    17701 tagctcaata tgcaacagta aaaacagtat caaatggaca aggtaaatat ccagtagcaa
    17761 ctaaccaaca agcagtgtta gcaacaaaag ctgaacttgc tgaaattggt gatattgacg
    17821 ctgaaatgtt tacttcagtt gattataaag tagaaactcg tgctggtaag attgccttat
    17881 caaatgaggt tgtggaagat tcagcagtga atattgtaca agaggtcaaa gatcagttag
    17941 cgaaattggt agaaaacact gacaataagc atatcatgga tttattaaaa acattcacta
    18001 agaaaacggc tgctacgttg gacgatttga aacaactata caatgtggca ttagacccag
    18061 cattaaataa aatggtaatt ctaaaccaaa gcggatatta ccacctagat acattgaaag
    18121 attcagatgg acgttacatt ttacaacccg atgtgacagc acctagtggt aaatcattat
    18181 tcggtatgcc agtagtattg attgcagata cattgtttgc caatcctaaa gctggaacat
    18241 tccctatgat tatgggggat attgcacaat ctatctttgt tgctcgtaga aatcaagtaa
    18301 cgactcaatg ggaaaaattc gattactact cacaaggact tgcagtgatc gttcgcaacg
    18361 attataagaa aattgatcaa aatgcttcag tgtatattga gtttacgcca gttgtaacac
    18421 caaaagtata gaaaaatatt gggcggcggt ttatcccact gccttttttt cattaggaga
    18481 gataaaatgg taattttgga tagcataaaa aaaagtatgc ggatcgatca cactattgat
    18541 gatgacttta ttcaacaatt gattgataca gcaggcgaat atataaaaag tgctattgat
    18601 agtagcgcaa cggataaaga tatggataat tatcagcaat ttgatttggc ggtgtcatta
    18661 cttactcaac attggtattt gaaccgtcaa gaagccagca gcgaacggat accagtaacg
    18721 gtacaagcat tagtacaaca aatgagaggt gcttattatg ccaatcatta agaatgtaaa
    18781 tgaattgact gagagagtca agtttaaaaa gacaaaacgg gtaaaagatg aagatgggca
    18841 gatggtggat agtgaagaaa ctgtatttga atgttgggct aatgtgcgtt cacaaatgct
    18901 aaaagacgtg cttgctagtg taggtaccat tcttgaagga acgttgacgt ttattattcg
    18961 atacgatcaa gattatgaac taacaaacga tatgaaagta gcttggaaaa acaagagtta
    19021 taaaattatt tcaatcaatg aaggaacggc gtttaaagat tatacaacga tcatagctaa
    19081 acagatttct tagaatgatt acacttgtaa acgttgtggg tatttagtat aattaaggta
    19141 gtaaatgaag ggttagctgc ctgaattttg caagacttag ctagtcgaaa tctattgttg
    19201 gactgaaaat tgtaggtgtg gttgcaaaca tgctggacta agaaagatga tagatgatga
    19261 acgttcaatt ttaatacagg catgctagga ctgattggat ggttttggca agctgattgt
    19321 gtagaaatca aagaacacgt ctgttgtgga cgtgtttttt ttgttataat tcaaaagagg
    19381 tgatttaatg gacaaagcag agtttacaaa tttttattgg aagtattatc tacatttaga
    19441 aaatgagttt attaatacca cagattttgt gatgctcgat gagaataatt tcaaaacatt
    19501 ctctattgaa tatcaaaaac tactactagc gattggttcg gagtgtgaga ttattttcaa
    19561 agaactttgc ggatttgata gcagtagcaa taaaagcata actgatttta aagctgttat
    19621 taaatctagc gaattaatgt gtttggataa tggtgttagg gttatgaatt ctttaacatt
    19681 gacttcacta aaaccttttg gagatgggtg gcctgaagat acaccagatt ggtggaaaat
    19741 atataacaga gtaaaacatg gtcgttcttc aaattataag gaagcaaatt tggaaaatgt
    19801 gttgtatgct ttagcaagtc tatatttatt agaggaatat ttgcataaga aagtaatact
    19861 agaaggtgaa gtagacttta taagtccaga gtcaaatcta tttacgttat cttgggaaag
    19921 aaaacattcg tcgatgcaaa aagtgacgtt tgaaaaagtg gattcaaatt atgtagctcc
    19981 actcattgat tttaaagaac aagaagaaat ttaaaaaccc ccacggctta ttgaagtttt
    20041 tgtatgccat attgtatgcc atttggttta aatcgattga aaaatagtga tagataactc
    20101 taattcattt agtcataaac gctgttttga taacctttat agttctttga tatgggtttc
    20161 tcattcatgg cggaagaaga agaatagaac aaaagaatga attaataaga tataaaaaaa
    20221 gggattgtga caaacgtctt gtcacaatcc ctttttccaa ataaacgatg ttcaatcagc
    20281 tgcccctcgg cattcgctcg aaaaacggcg aaatatgaag aactattttg ccatttgttt
    20341 ctctaataat caggtcaaaa cgttgacttc acagtctctt ccttaaacgg tgttctcctg
    20401 ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt ttttgaaatt
    20461 gttccttgtg actcggagct gaacggcagg ctcacgacct ctttcttaaa cggtgttcaa
    20521 aagcttgcat ctgcggtaat aagttcaacc aaacgaaaat tatgaggagc tatttttgtt
    20581 tggctgttct tattacttga tgcaggacag ctttttcaca acctcttttc gtttagcact
    20641 aaatttcctt aaaaagtaga aaagctttcg aagcctcgtt tgtttctttt actgtataat
    20701 aaaagaaaag agtaacaatc gctaaaagat ataaacaaga gaaaggaaat acaagatgat
    20761 tggtctgatt tattttgtgg tgatcgttct ggcgaacaca gttggcgcta tttccgggat
    20821 gggtggaggc gtgttgatca aaccgattct tgatcttatc ggcgcccatt ctgttgcagg
    20881 gatttctttt tactcaacag ttgcggtatt cacgatgtcc attgtttcga ctgtgcgaca
    20941 agtatcaagc gggaaatcac tgaattggca gattgtcggt tgggtatcta gtggagctgt
    21001 tgttggagga gtagtcggca acattgtatt cgaagttttt ttacagttat ttgaaaacga
    21061 gaagcatgtg cagctgatcc aaattttttt gacggtattg accttggttt ttgcgttctt
    21121 ctacaccaag catcatcagc caaaatttca tttgacgtca tggacttggt accttatatg
    21181 tgggggggtt ctgggctttt tagctagttt tttaggtatt ggtggtggac cagtcaatgt
    21241 ttctttgttg atgctgatgt ttgcattgcc aataaaagaa gcaacactat attctctatc
    21301 tacgattttc ttttctcaac tggcaaaatt ggtaacgatt gctttaacaa gtagctttat
    21361 gcgctttgat ttatctatgt tgttttatgt tatccccgct gctgttattg gaggtttgtg
    21421 gggagcaaga tttagcaggg ttttgtctcc aaaaaaagtg acctttattt ttcaagtaat
    21481 tgtcctcgtg gttcttttgg tcaattgtta caatgccttt gtcatcttac aaacattagg
    21541 agaatctaga ctaacgcaag tcaaaatcac tgaaacatat tgttctaaat gaaaaaaaga
    21601 gtatactatg ataaaaaaat tcattttaaa aaatcagaaa ttggctggtg atttggatgt
    21661 tacgggaaat gcgtttagaa gatgcagaag cactacgtga aatcaatgcg gatcaattag
    21721 ggtatgatat accattaacc gttacggtga gacaaatgaa gaaactgctg aaggagccag
    21781 agaaaaattt tttcttggtt tatgaagatc ctacttctaa aaaagcagct ggctatgtac
    21841 aagcggagtc ctatgaatcg atttttagcg atccaatgtt caatattatg gcacttgctg
    21901 tgtctaaata tgctgaaaaa agaggcatcg gaaaggcttt gatgactggc gtagaagaag
    21961 aagcgaaaaa acgaggaata acgacaataa ggcttaattc tgcagaatac agagtagaag
    22021 cacaccaatt ttatgaacac atcggttatc attccgataa aatgcaaaaa agatttttga
    22081 aaatcctgta aatctttatc aaaaaaatac ccgatcacca agccgatact tgatgatcag
    22141 gtatttttgt ttttttgact aagaaagtaa ttgcatcaag aaaccagaga tgatcaaaac
    22201 gatggcacca ccaagtcgat tgcccatttg agcaaaagca ataagttcca tccgattagc
    22261 tgcagataag acggctacat ttcctgttcc gcccatcgag ttgttgcata agccagcagt
    22321 aatagttgat tcaattggat ataaaccaaa caaacgaccg acaaatcctg aaacaactga
    22381 aatcgcgata acacttgtca gacataagac aacaaattgc catgtcaaag cagaagctaa
    22441 tacattcaga ttcagcaacg caataccgat accggcaaga acagctgctg tcaagttttt
    22501 gacaatcaag ttattgaaca tgatagctgc atcttcataa tactctggta ataggttggc
    22561 gattttacag atgacaacga taataatcat aaatgcgtaa gcatggactt taggaacaaa
    22621 atagttacca atttgtccta agatgaagaa agtcaatgat accatcaaac caacacctaa
    22681 ctgtggaatg gaaggtttca cagttcgagg ttttccatcg ccaagatcgc ctttttccat
    22741 taatttacca ttgccgttga attttggcat tgattgtcca gcacgagcaa ctaatgctgc
    22801 accgatgata gctaagacat ttgtcattgc tgtcgcagga atcaggcggg aaatgataga
    22861 agaagcatcc gtacctaaaa catttgcata aatcgttgat aaaggaacgg ctccggctcc
    22921 aacaccacca gccatcgcag gtaatgagat gtataaaacg gaatttgcaa aaccatttcc
    22981 gataagcatg cccacgagtc ctacggcaaa aaaggagacg accattgaaa taagtgcaac
    23041 gggaataaaa cgaacagaag ctttcatcaa tagattacgg ttcataccaa ggatgctacc
    23101 agtaatcaag gcagcaatat aaaagtccca aaagcccata ttgctgacaa agttttctgt
    23161 ggatttcact acatattcag gtaagatccc aaatgtagca atcgcagcag aagcgaagat
    23221 cgcaaaaacg gaaccaccac ccaaataact tttgacgata gggatttttg tcccgataaa
    23281 atgaaataaa ttaccgaaaa taaccaaaac tgcgattggt ccaatcatat tcgtcggtaa
    23341 tttccctaaa gccattgcaa tgatcaggac aaggaccatg atcaaataaa gtggcaaact
    23401 tacgccggtg atttttgtgg accaaaaacc ttcttttttc ttttcctcaa ctgcagttgc
    23461 agttgttgtt ttttgtgcca tttttacaga tcctttctta ttctgtatca tatgaaaaaa
    23521 gccaaagaaa agcgacaaaa gagcaggagt tggctggccg ctgttttgcc gcagtcgctt
    23581 ttttcttttt agacgtcata caaagtttct gtatgttctt ttactataac gatcttaatc
    23641 ttctaaagat ttatattcag gaacccattt agcagattca actgcttctt tgatatccgt
    23701 gatttcttca cgattcaagt tttgtgcgac aactgatttt gcgacagttt cagcaatcgt
    23761 ttgagaaaat tcagtgattt ttgctactgg aggcaagata gctgctcctg gttttgttac
    23821 atcaacgatt ccgcctaatg ctcgactagc ttgagaaatg atttcagcat ttactcgtgt
    23881 agctgttgaa gcaatcaaac caagtcctaa acctgggtac atcaaagcat tatttgcttg
    23941 accgatttgg taagttacac cattatattc aacatcagca gcagggatac ctgttccaac
    24001 taatgctttt ccgtccgtcc attctattaa atcttttgct tttgcttctg ccaattttgt
    24061 tggattagac aaaggcatga tgatcggacg tggtgtatga gcagccattt ctttcacgat
    24121 ttcttcagtg aatgctccag gttgggtaga agtaccaatc atgattgtcg gatggatcgc
    24181 ttttacaact gcttctaagt ttgtcaattc atcagcatta gtgaaatctg agcgtttgcg
    24241 agcgaatggg atttgaccag gagtcaatcc ttctgtatct tcaaacaata aaccttgttt
    24301 atctacttga tagaaatgtt ggcgtgcttc ttcttcaggt acaccttgac ggatcatctc
    24361 atctaaaagg atgtttgcga tacccacacc agcagtacca gcaccaaatg tcaaaattat
    24421 ttgatctttc aattgttcac cggatatatt caatccgcct aatacacctg ctaggacaac
    24481 aattcctgta ccttggatat cgtcgttgaa agtagtgatt tgatcttcgt atttttctaa
    24541 gatcgctgca gcatttcctc gtccaaaatc ttcccaatgc aataacaatt ctgggaataa
    24601 ttcggtagct gattgaacga attgatcgat gaattcatag taagtttcgc cttctacacg
    24661 agcatgacga ttgcctaagt ataagggatt attcaataat tcttcattat tagtaccagc
    24721 gtcgatcgat acaggcaaaa cttgtgctgg attaatacca gcagcagcag tatagaccat
    24781 caatttgccg atagcgatgt caacaccgtt cacaccccag tcgcccatac ctaagattcc
    24841 ttctgcatca gtcacaacaa tcaaacgaat gtctcgtcca tctgccgcgt tcttcaaact
    24901 tgctttgatg ttttctggtt cgtcgattga aagaaatgca gcatcttgcg gtttcaagaa
    24961 aatttcgttg tattgttcga ttgaatccgc aacgacagga tcataaacga caggcatgaa
    25021 ttcaactaaa tgttcgccca tcaatttata aaacaatgtc cgattcgtat tgaatagatt
    25081 cattaagaag atacgttttt ccaaatctgt ttgtttgctt aagtattgac cataagcttg
    25141 aacagattgt tgttccagtg tttggacagt acttggaagc ataccagtga ttccatattt
    25201 tgcccgttct tcttttgtaa aagctgttcc tttatttaga aaaggattat ttaataagtt
    25261 taaccctgta agcatgaatg attcctccta agttttaata caaacgaagt atagaagtca
    25321 gatgatgata tagtcaaaaa gagcaggtat gataaatttt atttatatgt aatatagcca
    25381 aaagaaatca aaatagcagt atttgttctt tttatatcga caatccttgc ttgaatacta
    25441 tgcgtatctc ttatatgcta gagaaagaaa agtagaagaa taagggatag atggaatgga
    25501 taaataggat ttctgaggaa aaaagcacat gatagcgttg ataggagtca cctatgtcag
    25561 gtgataaaaa tacataagta gaaaattaag aggaggaaga gaaaatgaaa gaagttcaat
    25621 tgattagaaa aagcgaacta tcagaaggcg gttgcaatgc atgtggcgta gtagaagcga
    25681 caagctatac attaaaatta gattccaatc aagcaataat ctctgaactg acagtaggcg
    25741 gtcttgtgga cagtctggcg cttgctgaag gttttactgg agaagatatt tatgagatgt
    25801 tttcagaagt ccgtcagtta aaaaaaggag aaaaatgcat agaggttcat catgagagcc
    25861 cgaatgtgag gttcaaacgt ggtgataatg agatgatctt caagaatcat gtgtctgatc
    25921 acactgaact atatgaaatc gtgaatcaaa tactgtctgg gatttttgaa ttaggacctt
    25981 atgagttcaa ggaagaaaac ggtaatccaa aattaaatga agaatggcaa gaaacgattg
    26041 aaacacaaag aaacaatcca cacttatttc aatagtcttc cacagtattt cgtaaaaatg
    26101 ttgatgaaaa agaaacaccg tgaatcagag gtcgtgaaaa acctgccctg catcaagtaa
    26161 taagaacagc caaacaaaaa tagctcctcc tatttttcgt ttggttgggc ttattaccgc
    26221 agatgcaagt ttttgaacac cgtttaagga agagactgtg aagtcaacgt tctgacctga
    26281 gtattagaga gacaaatggc aaaatagttc ctcatatttt gccatttttt gagctaatac
    26341 cgagggtcag ctgattgaac accgttttat cgagaggttg tgaaaaagtc gcttagtttc
    26401 gagtagtaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttaa
    26461 taccgagaaa atggcttttg aacaccgttt attcaaaaaa agggactttg acactacttt
    26521 tgtcaaagtc ccatcggttt tttatctatt tttattacaa tcatttcttc cagagtagtg
    26581 acatctggaa tggctgaaat gtaaacagat tatttgcctg tatcaatgtc gaagagtaat
    26641 ataaaaagtg taaacgctat catattgttc atcattcaaa ttttatagag agaaggcatt
    26701 gagatggaaa aaacatatca agcaggtaca aatgaaggaa tagtagattt tatcaatatg
    26761 gaagatttag aaatcgcggc ttcacaagtg attccagctg gtggttatgg ttacatcagc
    26821 agtggtgctg gcgacttatt tacttatcaa gaaaatgaac gggcattcaa tcatcgtttg
    26881 attattcctc atgtattaag agatgtagaa ttacccgata caactacaca ttttgacgaa
    26941 gaaatgctga ctacaccaat catcatggca ccagttgctg ctcatggatt agctcatgtg
    27001 aaggcggaga aagcttctgc aaaaggtgta gcagattttg ggacgatcta tacagcaagt
    27061 tcttacgctt cttgtacttt agaagagata agagaagcag gcggggagaa agcaccgcaa
    27121 tggtttcaat tttatatgag caaagataat ggaatcaatt tggatattct tgaagtggcc
    27181 aagcgtaatg gagcaaaagc aatcgtattg acagcggatg caacagttgg cggaaaccgt
    27241 gaaacagacc gcagaaacgg gtttaccttc ccgctgccaa tgccgatcgt tcaggcttat
    27301 caatcaggag taggccaaac gatggatgct gtatataaat cttccaaaca aaaacttagt
    27361 ccaaaagatg tagaatttat cgctgctcat tctgatttgc cagtctacgt gaaaggtgtt
    27421 caatcagaag aagatgtcta tcgatcatta gaatctggtg ctggtggaat ttgggtatct
    27481 aaccatggtg gccgtcaatt agatgggggg cctgctgcgt ttgattcgtt gcaatatgtg
    27541 gcagaagcag tagataaacg tgtaccaatc gtgtttgaca gcggtgtacg acgtggacaa
    27601 catgttttca aagcaatcgc atcaggtgcc gatcttgttg ctattggcag acccgttatt
    27661 tatggattgt ctttaggagg aagtacagga gtccatcaag tgtttgattt ctttaaaaca
    27721 gaattggaaa tggtcatgca attagctgga acacaaacag tagaagatat caaaaaaata
    27781 aaattgcgtg agaatcgttt tatttaagaa ataaatgacg aaaaaaatag gagtgcggca
    27841 gatgatgccg tactcctatt tttatagttc aaggatgtca ctaatagaat aatttgaaac
    27901 gaaaaaataa aattgaaata ttttttcata cttaataaaa aaggaagaat attcatgtaa
    27961 acgattttat tgtttttttc tcttttatac aaaaagggtc ataatttcaa gtaagcggat
    28021 gaattcatga ggattttttg catactgtct ctactatact taggtcacta agaaaaagga
    28081 gtgatttatt atgaaaaaga acatgattgg gacattatta ctttcaacac ttcttctagg
    28141 cggcttggca acaccagctt ttgctgaggg tcaagcgact tcaaaaggag atattacctt
    28201 tactgaacca acaaataccg ttgagcctct taaccctact gatccttcaa aaccagtaga
    28261 gccagcagat ccggaaaatc cagcaactgg ccagacaggt tctttaacat tggatgtagt
    28321 tcctgaattg ccatttggta cacacgagat tgaaagcgga acaaaaacgt atcaagtaga
    28381 cgcttcaaaa aacgatacac cttatctcca agtatcagat cgtcgaggtg taggtgcaga
    28441 tggccaagca caagggtgga acgtgacagt ttcggtttca gattttgtaa acggttcaca
    28501 agttttgcaa ggcgctgaat tagattttgg aacatccact gttaaaagta cgtcagataa
    28561 cgaatccact ggaccaacta gtcaaactgt aactgggttg agtaaagcaa gtgcagcgac
    28621 accaatattt acagcagcaa aagatcaagg tctgggaaca tggctttctg tttatgatcc
    28681 agcaaatatc acactgaaag tgccgaaagc agcagctgga acattcacgg ctgatttgac
    28741 ttggaacctc gtggctggcc cagtagcata gagtatgaaa taaattcaac aaactgcggt
    28801 gaaaatacta taggtgaagg attttttacc gcagttgttg ttgaaaggat gtggtggaca
    28861 atgaatcaaa gtaaacgact gtttgtttcg tttttttcta ttttaagtct atttttcatt
    28921 tttccgtcaa tcagccaagc aaaggattca gcaggagact ttggaatcaa accggtattt
    28981 ccagagaacc aaatagataa agcaatcggt tattttgatt tattagtggc accggaacaa
    29041 aatcagacac tagaagtaat tatcagcaat tcttcagatg aagaacgaac atttgaggtc
    29101 tctgtcaatc cggcggtaac gagtgatggt ggaacgatcg attatagtca aaaaaaccca
    29161 acgttagatg agacattgcc gtttgatgtc cgtgacgttt tgctcattgc gaaaaaggaa
    29221 atcaatgtat cagcacatgc agaaacaact atacctatag aagtgaaaat cccagcaaaa
    29281 agctttaagg gacgtgtatt agcgggtatt catgtttcac ctaaagaaga agctgaaaca
    29341 gaaaatgcta cagaaggagc acaaatcaaa aatcgtattg cttataatct cgcagtcgtt
    29401 ttacaagaaa gtcaagagac gatagaaccg gatctgaaac ttttatcagg agatttggac
    29461 gaggtcaacg caaagccaac tgttcagctt cgtttccaaa atcctcagcc taggattatc
    29521 agcaatttga tatttacgag caagatcttt tatgaaaatc agctttatat tgaaaatacg
    29581 tctaatgaat ttttagttgc accaaattcg aattttcact tgaatcttga tttagctggt
    29641 gataaagcaa aagccggcga ttaccgcgca gaaatcatag caaaatcagg cgacagtaat
    29701 gaatggcgat ttacacaaaa ctttacgatc aaaaaagaaa aagcacaaaa agtaaatgaa
    29761 aattccgttt ttgcagtaca agaacaatct ttcccatggc tttatgcagg attggttgct
    29821 gcagttctag tgttatggtt gattttgctg tttttgtggg tccacaagaa aaagaaaaaa
    29881 ggagaaacaa catgatgagg tgatttcgtt gaaaacatcg atcttattgg gagggattat
    29941 tgcaagtttg ctgttcttca gctggcaaga gccagtccaa gcagatacca aaagttcagg
    30001 tacgattaca ttttatgatc taagtccagt tccgcatcca agtggttcag tcaatggcgc
    30061 atcaaaacag cagacacagc taccgattta taaagaatct aatcaacatg gcgtattccc
    30121 ggagacgaat gaagaaaaga tccgttttct ttatttgatc attggtatag aattatggct
    30181 tcttgcgtgg ttgttgttat ggagaagaaa ggaaaaggag gatgaacatg ttgctacaaa
    30241 ataaattttc attttttcgc tccttctttt ttacttcgct ttttgtttta agtatattta
    30301 tcttttcacc ttcggcagaa gctgttggag caacgcctcc tgccatttct tatgatccat
    30361 acacgtatga aaatatcgta cctccaaatg gtggacgtag tgcagtcaat ggcgggaagc
    30421 ttgtccaact aactggggct aaccaattgc ctaaattgac tgctacttat ttgaacgatg
    30481 gttcaaatga tccttggaaa ggtggcacgg tccgttttca ttgggaaagc catgtgaaac
    30541 taacaaatgt acttgatttg gtgaatatca ctgcacaagg atttgcattt gtggcgatca
    30601 tcaatttgcc ggaacatgta tcaagcagtg atgtattaaa agcaattgac tggaacaacg
    30661 cctacttgta tattgccggt gcccccatca aggttactag cggtagttta ttatcagcag
    30721 gaagccatac cttgcgtttt gggatggggt cgtgggatac aaatactcta ttggcagata
    30781 ttattgctat tattacgcga ggcgaattca acgtggacga tatcccgctt caatttgatg
    30841 ttacagctaa tgtcgcgaag atgacagaaa acggagataa atatgatacg agtccaaaca
    30901 aaattttgac caagggtaaa ttacaacctt ctatgtctaa aaaggccgtt ttttctgttg
    30961 atttttatga ttcgaataat attatccaag atcgtatgtt caattcgatt ttagctccag
    31021 ctgcaggtag attttatcct aaattaactg gcggaaactt gaatccagga gcatatgcat
    31081 tttctgcaag tgcagagata aatacgtgga atagttacat ctcaacaagt gattcaacag
    31141 gaaaatataa tacatttgct gataccacag aatacgtgga tggaaataac cagaaccttc
    31201 caggctctac caatagtcgg acattgacga tggggctgac tgaaggacgt tttgatcagt
    31261 tgccatttag tcgctttgat cgcgtagtca actattttac aggaaaaaac gttacacaag
    31321 gagcaatact gactcatact ccagcaagca caagtggatt gaatcagaca gccaatatcg
    31381 tgtacagtgg gaaagatgcg caaggcggat tgctttctcc tgttgcttta aaggtattgg
    31441 agcagtacaa gctagacgga acggttttac caacaaactt aacagccgat agtcagtggg
    31501 gaaagctcct ttcttctgca tcgtaccaag tcacggccaa ttggtcagca actgaactaa
    31561 aaaagggttc gatccattac aggctttatt cgaaaggaac ccaaaaatta gcgacaggat
    31621 ggacagatca acttttccaa acgatccaat caaataacgg gggtgtaaat acagctactt
    31681 ctacactacc tgcattggct tcagggcaat attattttga ttatcgcttg gtggacgatg
    31741 tacttactaa catgtatccg tcttttgcgt acaaatggca gtctgaacaa gctggaatca
    31801 ccactcttcc tcaaataaca gtggctaatt ttccatctgt gagtgcagat agtcagctaa
    31861 aaaaccttag cagacaacct gagacaggag atcccctgtc tgctctatca ggagatacga
    31921 ttcaagaaaa tgtgacattc acgttaacaa aatctgggga tgcgataacc gacaaaaaaa
    31981 tcacgatctc acttcctgaa aatactactt atatcaatgg ttccttaaaa ttaaatggta
    32041 caactatcgc agataccggc attcaacaag gagtttctgt cccagcagat ctattgagca
    32101 aaataggaga taccattcat cttacttaca actatcaatt aaatactgta gatacttctg
    32161 tacaatccgt cagcatactg acaaaggcag ctgtactttc cagcaatatt acattagcag
    32221 atggtgctaa gcttcctaac ccagttgtcc aaacatctgc taaaacaatt cttgtcccca
    32281 aacaagaatt aacattagtt aatgtaccag atgattttac atttggtaac gatctcccca
    32341 aaccgttgaa gacaagttat tatgaagcaa aaggcgattt ttcttttgat gtgcgggata
    32401 ctcggcttcc aagcacgtct ccttggcaac tgacagggac attgacttct ttatttaaga
    32461 atgatcaagg tcaagaatta agcggaacaa aattatactt caatcattct ggatcaaaac
    32521 aattgattca gcaaggtcaa aacacgttga tttatgagag tgatggaaca gctaaaggag
    32581 aggttctcgt tgactttccc gataccgatg gtttactctt agaggtaaat agcagtacga
    32641 atgctcaacc tggcgcaacg tatcaaggca tggtcacatg ggaactgaca gctgggccaa
    32701 ccagctaagt gattagaatt tgtatggatc tgtgataact gtcacagatc ctctttttca
    32761 aggatgagaa ggaaacttct gctgttaaaa tcagaaatgc ttggaggagt ggacttggaa
    32821 cttttcagtt tgcttgaaaa aaatgaacaa cggcaaatca aacttttcca acttttatta
    32881 gaggaaaaag gagagatttc cgttaataac gtacttaccc gcttatcagt tgatcgggct
    32941 accttgaaag aagatctgtt ttatttacaa gctaacttgg aaaatatcaa acaattggaa
    33001 attgttatcc aagaagggaa tattttatta atcagacatg gaaatatagc agcagtcgat
    33061 gtttattatc attgctactt gcgctattct acaaaatatc agatacttct ttatctgttg
    33121 gaaaatggct catttgatcg gcagaaactt gtcatggaac ttaatatcag ttcggctact
    33181 ttgtctcgca gagtgaagga attaaatgat tctttaaatg agtttcatct gaaaataaaa
    33241 agcgggcggc tgaccggtcc agaaagtcaa atttgttatt tttatttcca attattttgg
    33301 tttggacgtc cttatcttga aaacaaaaaa gaatacagtg atttatcagg aaacactttt
    33361 ttagatctgc tgcaaaaaga gtttagtttt actattacag aagaaggacg attgaaattt
    33421 gaaatatggc tttccattat gaagaagcga atgcgctgta cgaaaaaatc caaaccagat
    33481 tatagcaatc ttcctgttgg attgtttcaa acgacacaat ttttattgga cctccataca
    33541 tttttgaccc gttacttctt tcaatatgcc tttccttgga atcaaaatga aacgttggtt
    33601 ttttatcttt tcatgatcag taactttaca ttagatacac ataactcgca tgtccaggag
    33661 tttttgataa gcgaaaaata caaagacgac gtagtagctc aattgaatcg aacgtttaaa
    33721 caatcgatag aagaatattt ctctttttat gtttttccag aagagatttc gcaaaagatt
    33781 gcgttgacag tgatgcaaaa tcacgctcat tttgcctatt ttagaggatg gatgaatatt
    33841 tttggccaag aaggattaaa acagcggtta actgaagtag aagataaaca attcctggtt
    33901 ttatgtgaaa gattggtcag acaaacattt gctattgttg attttactga agtagacgag
    33961 gaaaatgccc gctttgaatt atttgctcgc tatgcatcta ttttgcggac gctgatcaag
    34021 cagttagaat tacgtctgca aattgcttat gatcttcctt atgaaaggct aatggcggat
    34081 atcgtgaaag aaatcatcca ggaacaattg ctgaatacga tcaaatatga tttagtcgaa
    34141 tacgagaaag aaaagcagta tgatatcatt ttgactagcc aattgaaaga gtacccgtca
    34201 cagaagtccg ctaaagtttt tgtattcact agcaacgaat ataattatga tttcccctat
    34261 ttgaaccaat ttttaaaaga atgttattta gaaaaattga atttaaggat gaataaaaca
    34321 tgaaaatcgt gatcattggt gcttcccatg caggcatcac agctgcccta aatctaagaa
    34381 aacttcaacc gaaagcagaa gttttactga tcgatgaaga tcataaagat ggactaggct
    34441 atgtttcaaa tggaatcaac ctttatttaa aaggaaagat acgtagttta gctgaagttg
    34501 cccataacat gcggactctt caagaaagtg gggcaaaact gatcaccgaa tggagagtaa
    34561 ctgaacttga tccagacaaa catcagctca tcctcgcatc caaagaagga aaaaacgaga
    34621 ccataaccta tgataaatta atcgtagcaa caggttcctc tcctgtcacc ttgtataaac
    34681 aaattgaagc tgaaaatgtc tacacctata aaaatctagt gcaatctaag caagtacttg
    34741 ccgctttaaa agaagcaaaa gaagtagtga tcttcggtgc tggttatatt ggattggaat
    34801 tagcagatgc tttgagaaat aaaggataca ttattcatct agttgattat atgccgaatg
    34861 ttttaacaag atatttcgat aaggatatga tcaacagttt tcaaaatcag ctgcaaacta
    34921 agcaaatcaa tttttatcca aatgaattct tgatcgattg gaaaaaatca gaagagaaag
    34981 tagtatccgt acaacttcta tctcaagcaa taaaggcaga tatggtcatt ttttctgccc
    35041 aaactcgtcc taatacgaca ttgttaaaag aaaaagtggc tttgtatgag gatgagactg
    35101 tgatagtcaa tgaatatctg cagacaagtg atccggacat ctatgcgatt ggagatatcg
    35161 tcccagtcag ttttgacaaa aataaacggc atctcttttt acctttagtt actcgagctg
    35221 tacatatggc aagagcagta gcgctaactt tgagtggtca gccaaccgct tacgacctca
    35281 gacaaaagat aacagcagca gtcattatag actacttttt aggaactgta gggctgacag
    35341 aagatgaagc gccattcttg gaacaaaata ccggttcgtg ttcaggagaa tttgatctat
    35401 ttccacaata cgatgaagaa aacaaaacag taaatgcaaa gctgatctac catccggata
    35461 cattagaaat aattggcgga cagctgataa gccaagaatt cttattatca gatttgaacc
    35521 tgcttgcgga tatcgtgaaa cacaaaacta cgattccaca gctagcagtt gaggattttg
    35581 gatttctgtc tgaacacacc tctcgttttc actacctcaa tgagttagct tttaaagtac
    35641 tagctaagag agcaaatagc aatagagtag ataaaagact gtgagaaata tcacggtctt
    35701 ttattgtgtg gggatataat aaatattaat caatcgagaa taaaaaattc atataatgaa
    35761 atagcggaag aattttttga gcataatatt cgccaaaaat ttatcaagtg ataaaattgt
    35821 tgtataaaat gattatttca ggaggaggag ttaaatgttt ttagcattaa atgaaattat
    35881 gcattcaaag ttacgttatg cgttagttgc aggggtgatg tttttgatcg cctacctggt
    35941 attttttttg actgggttag cttatggctt agctcaagat aatcgaacag cagtcgacaa
    36001 gtgggaggca gattcgattg tgctttcgaa agatgcaaat tcaaatctag ggatgtccat
    36061 gattactaag aagatagcag aagaagtcga aggtgggaaa gtggcgtatc ttgcccagac
    36121 acctggagtc gtgactagta aagacagtac agaagaagga aaaatcaatg ttagcttctt
    36181 cggtatcgat aaaaatcaat tcatcatgcc caatcttgtt gaaggaaaag cttttgataa
    36241 cgatgatgag gcagtaggcg atattagttt gaaagaagaa tatggtctag ccgtaggaga
    36301 tacagttaag ttatctggta gtgacaagac gtttaaacta actggcttta cggatcatgc
    36361 caaattcaat gtctcaccag ttctctatac aaccatcaat gcttaccaag agatccgctt
    36421 cgaaaaagaa gatacaagtg aaaatgcacg gattaacgca atagttgtca gaggtaaaat
    36481 cagtaattta cctgaagatt tagaacagat caaaatttct aaattcatca atgaattacc
    36541 tggatataat gcgcaagtat tgacttttgg atttatgatc ggatttttga tcgttattgc
    36601 ggctattgtg atcgggattt tcatttatgt actgacgatg caaaagatta atatctttgg
    36661 tgtaatgaaa gcacaaggga tcacaggagg atttattgcg cgttctgtag tggcacaaac
    36721 attcatttta tcatttgttg gtattcttct tggtctatta ggaacagtgg gaacatccct
    36781 tgttctacct gatgcggttc cattccaaag caattggctg ttttttggcg tcattagctt
    36841 gttgatgctg gtagtagctg tgttaggcgc actattttct gttcggacaa tcgtaaaaat
    36901 cgatccgttg aaggcaattg gataagaagg aggaagagca tgaaagcaat agaatttaaa
    36961 gaagtagaaa aaagtttcca agatggcgac caagtagtta aagcattgaa agaaacaacc
    37021 atgagtatcg ataaagggga atttgttgca atcatcggtc cttctggttc aggtaaaagt
    37081 acttttttga ctttggcagg cggattgcag acgcctagca gtggctctgt atttatcaat
    37141 gatcaagaat tcagtaatca aaaagaaagt gaacgcgtta aactacgttt taaagaaatt
    37201 ggctttattt tgcaagcttc caatctggtt ccttttctga cagtaaaaaa gcagttatta
    37261 ttagtagata agatcaaaca tgaacatcgt caaaaagaag caaaagaatt atttgagcag
    37321 cttggcgtgg ataagttgat caacaagtat ccagaagaac tatctggtgg agaacggcaa
    37381 agggtcgcaa tcgctcgtgc attgtacaat gatccgtcca ttattctagc agatgaacca
    37441 actgctagtt tggattcaga aaaggcacga gaagtagtcg atatcttggc aaaagaatca
    37501 aaagacaaaa ataaagcaac gatcatggtt actcacgata cacggctgat tgacaaatgc
    37561 gatagaatat ttatgatgga agatggtgta ttgaaagaaa aagaataacc aaaaagcagg
    37621 tggaaaaatg gtccatcgat acaacaaaga tacacagcaa aaaattttga aagaagcgac
    37681 tgacttgttt atgtccaagg gatatctcgg cacttcaaca agagagatcg cacaaaaagt
    37741 tgggatcacc caacccaatc tctatcatta tttcggtgat aaagaaaaac tatatacggc
    37801 agttttagaa aaccacttaa aagatgttgg aaaagctttg agagaaatcg ttcaaacaag
    37861 cgaaacggga tttcaagtaa ccttgacaaa aatggcgcag tttttgattg agacacatct
    37921 ggtcgatttg tttctgatgc ttcacgatct tgaaagcaat ctttcaaagg aaacaagaga
    37981 tcatcttttc tttctatgga agaaaaatta tcgtgaacct tttgaagaaa tcttttcagc
    38041 aaaccaatct gtgctaagaa atgggatttc aaaagaaatc gcagcaaggc attttttctt
    38101 agtgttagca ccttacatca ctcggtcgaa aagacctgtg gaagaaccgc taaatgtggc
    38161 gcaattgatc gatttatatt tgaatggtgt attaggagaa gcgtaaagct gttatatagg
    38221 gagaaaaata gttaggtgaa gaagcaagat atgacgaaga tcgttatatc ttgttttttt
    38281 tatataggta gtttttcata aatacaaatt tgttattctc tcatgattat cagggtatat
    38341 ttttatttat tatgtcggtg ttccaggaag tgttttaatt taataacgaa gaaattaatt
    38401 gtttgtagaa tgaaggaaac tatttattgg gtagatgaat caccacgaat agtcaaaagg
    38461 agatttcagg atatgccaaa aatgaaaaaa atcattttag cagtaatgtt attttctacg
    38521 gttatatctt ttacagcccc ttttttgcgt acttaagctg attttaagtt ttgaccctta
    38581 gtctggatct acgaggtgag agtcaagaat gaaaaaaata attggagctt ttgtatttgc
    38641 aatcattatc attattatct gttttcaaat agatgttttt ggtcaaaaaa agatagttgg
    38701 acaaacggtc agcagtacga acagtacggt tacaaagaca aaggaatcat ttgatgatac
    38761 agaacaagag acattaatca aaatcggaaa aaatgaccgt acactatata cagatgactc
    38821 gctaactacc ccacttgcaa ctataaacgg cggagagctg gcggagtttc tttctgaaac
    38881 aaaaaatgct tatcagatca agacaaatga tggctatact ggatatttag ccctcgaaga
    38941 tggaaccaaa gtcacaaaga atatacaaac aaaaccggaa gagctatctg atgcagtgat
    39001 tgtattagat cctgggcatg gcggaaatga tactggggca ttaagcaatg atgaacaaac
    39061 agaagaaaaa gatataacat taagtacagc cataaaagta aaaaaggcgc tagaggatgc
    39121 tggagcaacg gtttatttga cccatactac agatgaatta gtacagcttg gagatatatg
    39181 cgactacagt gaagaaaaaa aggcagatgt ctttatcagt ttacacgcag actctacgga
    39241 atatgcgaat gaagcaactg gaatcacgac atattattac tatggacaag aggaaacatt
    39301 agcacagaca attgctgact ccttcactga tctgcctctg gattcaagag gaatctccac
    39361 tgggaactat caagttcttc gagaaaatct tcaaccttct attttaatcg agatgggtta
    39421 tatgaataat gatagtgatt taaaggaatt agtaacagac agttatcagc agcagattgc
    39481 tgcaagtttg acacaagcat tgaccactta ttttcagtaa taaataagcc cagccaaaca
    39541 aaaatagctc ctcatatttt tcgtttggcc gggcttatta tcgcagatgc aagcttttga
    39601 acaccgttta aggaagaggt tgtgagcctg ccgtttaact ccgcgtcaca aggagcaatt
    39661 tcaaaaaaca gcttctcatg tttgatgact attgtgactt gtgaccgagg agttggcatg
    39721 agaacaccgt ttattcggaa caagggactg tgacatgact tttgtcacag ttccattatt
    39781 tttttagcag cttcaaagct ttttctgtat tggcaaaatg aagtttggca tatttttcaa
    39841 gcataggcat gccgcctttc tccaatagtt ttgccgctac ttggtcagat ttcgttccag
    39901 ctcctgatgg aagggtgatt ccagcttcag ctgatgcttt gtgtaactct tccaaaaagc
    39961 tggcacgact gatgatagaa gcagcagcaa cagctaaatg atattgttct cctttagtaa
    40021 cgaagtacag tttttcttgt aattgatttt tttctaaacg cgcatatttt ttaaaatttg
    40081 cttccggcgt aaactgatcg atcaaaatcg cttctggttt ttctggctcg atttttttta
    40141 gcagcaaatg gatcgcttga ttgtgcaaag cgactttcat ccgaacggca ttgtattttg
    40201 gttggatctc attgtatttt tctggagata agacaagcag tttatagggg atggtttttt
    40261 ttaagatgtc agcaagctgg atgatttggc tatcttttaa ctcttttgaa tctctaacac
    40321 caagtttacg taattctgca agctgatcac gtttcacata agcagcacat actgttagcg
    40381 gaccaaaata gctgccattt ccgacctcat cactacctaa aacggataat tgactgatat
    40441 tttttggcaa agaagaggct gtttttgctg gcttggcttg tttatcctgt gcctcccccc
    40501 atttatcagc ttctattgct gcattcgtac cttgaaacat cacttttccg gatgtataag
    40561 cggtaatggt cgtcccgtct ttttttgcag caaagctagt ataaggaatc tgtttattta
    40621 acaaataaga gcgatagtat gctttcattt gactaagttg gtcgtttgtt gctttgataa
    40681 tgacgttgct cataaaattc tcctcgtttt tatacataaa actataccgc aactcacgac
    40741 ttttcgtttt ctaagagatt gcgagacata gcattttttt aaagtttatc atataatagt
    40801 taagtatgtg aaaaatattt gtggagaacg gtgaaagaac caacttcatg gtacactaaa
    40861 taaaactttc atcaaatagg aggaaatgat tcgtatggcg cacgaaaaaa cacgctacaa
    40921 agcagtgatt gccaaccaaa catatactat tatcggacgg gaaaccaagc atcatatgga
    40981 tatcgtaacg aagctgatca atgaacagtt agcagaattg aaacaattat caccacaaat
    41041 ggataatgaa caagcagcaa tcttgatggc tgtcaatgcg ctttcggatc aattgaagaa
    41101 acaagaaaga atcttggagc tggaagaaga aacagcagaa ttaaagaaaa aaatgatcaa
    41161 atttacagag cttgaaaatc gtgtgaaacg cattgaagca atcgagaacg aagctcggga
    41221 agtattgaaa gaaaatggtc aagcagatca taccattaaa aatcatgtgg aagcacaaca
    41281 gattttaaac gagaaacgaa aaggacaaat taaacaaaaa tcttctaatt aagatccttt
    41341 tatcaaaata agaaaaattt aaaaaagtta gaagagaaag gaacgtagca tgctgagttt
    41401 gctgatttta tttttattgc taattgcgtt tttttctgga gcacgtcgcg ggtttgcttt
    41461 gcaaatcata ttcgcgattg gatatgtatt gtcatttatt gctgcccagc atttttacaa
    41521 accattagca agtcatttgg aattatacat tccataccca gctgtcaccc caacatcaaa
    41581 gttagcattt tttgatcagg tatttgcttt tcacctagat gaagcttttt atgcaggaac
    41641 tgcttttctg attattttgc taattggctg gctgcttacc cgatttgtgg gggtttttgt
    41701 ccatggattg acgtatgtgc cgatcttgag acaggtagac tggattgcag gaggcatcct
    41761 gtcactaatc atggcttacg ttactatatt tttgattttg cagttacttg cctttgttcc
    41821 attagatgtg gtccaaaatc aattcggcga gcatagtttc gctcgtttta tcgttgagag
    41881 aacgccattt cttaccaaca agatgtatga gctttgggta acgaatattt tgaattaagg
    41941 aaaatagtgc tggaaaatat ttcgtttttt gtacagagta gtttatttaa gaaggctgcg
    42001 tagcgtatgt ccggcacagc tcttttttca tagtgaaaga taaggtgata aaatgaataa
    42061 ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag atggtcagac aatttgtagt
    42121 gactgcacaa ggaaaagaag aactggcaga attggtacca gtgagtgaga aacaaacgat
    42181 cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc gaggcgggat
    42241 ccctatacct aaacttgaaa atatccgtcc ccatatgaaa cggatcgaga tcggagcaga
    42301 cttgaatgga gtggagcttg cacaagtttc cagagtattg tctacgacaa gcgaattgaa
    42361 tcgtttcatt gatgatcttt ctgacagtga aatcgaattt gcacgtttgt atatgtgggc
    42421 agatcagtta gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa tcgatgaaga
    42481 cggacgcgtg actgatgacg cttctcctga gttaaagagc ataagacaga acattcggcg
    42541 aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa atgcaaagta
    42601 tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac gttatcccag tcaaacaaga
    42661 ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt gcttcaggac agactttatt
    42721 tattgaacca aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc aaatcgctga
    42781 acgaaatgag attcaacgta ttttaagtga actttctgct gaacttgttc cccatcgtca
    42841 agaaatcatc cacaatgctt atgtgattgg taaaatggat ctgatgaatg caaaagcacg
    42901 atttggtaaa gaagtaaaag ccatcgtgcc gggaatcagt gaagacaatc atgtcgtctt
    42961 gaaacaagca agacatccac tgatcgatca ggaaaaagta gtttctaatg acatcacaat
    43021 cggaaaagac tatcaagcca tcgtcattac aggacctaac acaggtggga aaacgatcac
    43081 gttgaagact ttaggtctgt tacaactgat ggggcaagca ggattaccga ttcctgcagg
    43141 tgaagaaagt caaatcggta tttttgagga agtatttgcg gatataggag acgaacagtc
    43201 gattgagcaa agtctatcga ccttttcttc tcatatgacg aacactgtag atattttatc
    43261 aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa cagatccgca
    43321 agaaggtgct gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt ctgcttatgt
    43381 gatggcaaca acgcattatc ctgaattgaa agtttatgga tacaatcggg caaatacgat
    43441 taatgccagc atggagttcg atgtggatac actaagtcca acgtatcgtt tgctgattgg
    43501 cgttcctgga cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg atacagaagt
    43561 tattgatgaa gcgaagcagt tgatgaatga tgagagccaa gatttaaacg aaatgatcac
    43621 agatttagag aatcgccgga aaatggcaga gaccgaatat ctggaaatgc gtcattttgt
    43681 ttctgaggca caagaacttc acgatgattt gaaagaagct tacagctatt tctttgaaga
    43741 acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaac gaagtcgtgt cagaggcgga
    43801 agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga ttgggcaagg
    43861 aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc tacatcaaga
    43921 agaaactttg aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa cgttgaaacc
    43981 gggagacgaa gtgttggtta cgacttacgg ccaaagaggg acgcttttac gtaaaaacgg
    44041 gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag acgagctgac
    44101 gcctgttgct cctcaaaaag agccgacaca acgagttatc catgctgtac gatctgaatc
    44161 tagtagtcac gtgccgaatc aattggacct tcgaggaaaa cgatacgaag aagcgctaag
    44221 tgaggtagat caatatttgg attctgccat tctagctggt tatcctcaag tgactattgt
    44281 ccacggaaaa ggaacaggcg cacttcgcaa gggaattact gattatctta aaaaccatcg
    44341 gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg cgacaattgt
    44401 taaatttaag taaagcttac taaaggtaag cagatggttc gctatccaag tatagaaatg
    44461 ctatactaat catagatttt aaaaatggag gtctttgata tgtcacaagt aattacagat
    44521 aagacattta atgaagaaac agataaagga cttgtattga ttgatttttg ggcaacttgg
    44581 tgtggtccat gtcgtatgca agcaccgatt ttggatcagt tggaacaaga atatgatgaa
    44641 gaagaattca gaattgctaa aatggatgtg gatgaaaatc cagaaactcc tcaacaattc
    44701 ggtatcatga gcattccaac attaatgttg aaaaaagatg gtcaagtagt agaaaaagct
    44761 gttggtgttc attcaaaaga acaactacgt caaatgatcg atcaatattt ataaaaactt
    44821 acagcaaaaa taagctgagg gtgggactga atccagttcc gtcctcggtt tttttgcggt
    44881 tgagttaagg ttattcaaat taatattttt tgagtataat tacgttgtca ggctggattg
    44941 tttttttgac agacatgcta taataacttt aattgaatat gaaggggagt accagatgaa
    45001 cgaacgaatc aaaaacaaat tagctttact tcctgatcag cccggctgtt acttgatgaa
    45061 agataaaaat ggaacgatca tttatgtcgg caaagccaaa atattaaaaa atcgtgtccg
    45121 ctcatatttt actggcagcc ataatacgaa aacggaacgg cttgttagtg aaatcgaaga
    45181 ctttgaatac atcgttacag aatcgaatat cgaagcattg ctcttagaga tcaatctgat
    45241 aaagaaaaat gatcctaaat acaacattat gttaaaggat gataaaacct atcctttcct
    45301 taagataacg aatgaaaaat atccccgttt ggtgattact cgtaaagtac taaaagacaa
    45361 agcactgtat tttggaccat atcccgatgt cggtgcggcg aatgagacaa agaagatcct
    45421 cgatcgatta tttcctctga gaaaatgcaa acctagtcag acgaaggagc cttgtcttta
    45481 ctatcatttg ggacaatgtc tgtgtcctta ttatttcgac gtagatccga aagtctatac
    45541 gaaaatcgtt gatgaagtga aaaagttttt aaacggagga cacgaaaaaa tcgagtcaga
    45601 gatccatcag aaaatggaaa aagctgctga aaacatggag tttgaaaaag ctgcagaata
    45661 ccgagaccag atccgtgcca ttgagacaat catgactcgc caaaaaatga caaatactga
    45721 tttactggat cgagatgtat ttggttatgc gattgacaaa ggatggatgt gtgtccaagt
    45781 gtttttcgta agacaaggaa agctaattga acgagatgtt tcaatgtttc ctttctataa
    45841 cgaagcagaa gaagatttct tgacttacct cggtcagttt tatcaggaaa atgagcattt
    45901 cattcctaaa gaagtcttga ttcctgatac gatcgacaag cccagtgtag aagctttgtt
    45961 agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa aaattagttg aattggcgaa
    46021 taagaatgcc tcagttgctt tgaatgaacg gtttgatctg attgccagaa aacaggaacg
    46081 gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc caatacgtat
    46141 cgagtctttt gataactcta atatcatggg gacgaatcct gtggctgcga tggtcgtatt
    46201 cattgatgga aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa cagtcaacgg
    46261 accggatgat tatgcatcaa tgagagaagt catctatcgc agatattcac gagtgattcg
    46321 cgaagggttg cctttacctg atttgatctt gatcgatgga ggaaaaggac aagtagatgt
    46381 ggcaaaagat gtcttagaaa atcaattagg aatcgatatt cctattgctg gcatggcaaa
    46441 aaacgataaa cataaaacaa atgagttgct attcggtata gatttggctg tagtaccgct
    46501 tgagcgaaat tcaccagagt ttttcttact ccagcgaatc caagatgaag tccatcgatt
    46561 cgcaatcact ttccatcggc agctacgcag taaaaacagt tttgcttccc gattagatga
    46621 aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca aatcgttgaa
    46681 aaatatcaca gcagccacga cagaagaatt acaagaaatc ggactgccga aaaatgtcgc
    46741 acaaaacgta tttgataaat tacatcagga atgaggaaag ggcactttac aaagaaagta
    46801 aagtgtccta tcattttatg tacaacaaaa agagcgttta cgttttgttg gtaaataata
    46861 aatcgcttac gtctggtgtc taaggatata tgtgatttct tgaagaagga gtgaagaaat
    46921 gttagaagtc aaggatcttg ttaaaacttt tgggaactac acagctgtcg atcatgtctc
    46981 tttccaaatc cccgatggaa aaatcatggg gctaattggc caaaacgggg ctgggaagac
    47041 aacgactttt cgtttgatct tggatttttt gacacaagac caaggagaag tgttatggaa
    47101 tggccatcat cttggggaga aagattatga tgtcatcggt tatcttccgg aggaacgggg
    47161 actttatccg aaagtaacga ttcaagacca actgctttat tttgcagaac ttagagggaa
    47221 atcaagaaaa gagattgaac caaaaatcga tttttggatg gaaaaatttc aggtaaaagg
    47281 gaaaaagacg gataaagtaa aatctttgtc taaaggaaat cagcaaaaag tccagctgat
    47341 cgccacgtta atccatgaac ctaaattgat cattttggat gagccattca gcgggttaga
    47401 tccggtaaat gctgaattgc taaaagatgg aatattggaa ttgaaaaaac atggttcatg
    47461 tgtcattttt tctagccaca atatggataa tgtagaaaaa atctgcgatc atttgatcat
    47521 gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg aatcatttgg
    47581 ccgtacgaag ttattcttgg aatctggttt aagccaacag gaagtagaag aaatcgaagg
    47641 cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag attaccttag aagatcctga
    47701 agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc tacattccta tgtttaacca
    47761 gcaaccgcca actttggaag agattttcaa attgaaagca ggtgaaatcg atgcgtaagt
    47821 tttggattat taccaaagat gtctatttaa aaaatgttaa gtcgatttct ttcttgatca
    47881 tgatattagt cccttttatc atgctgggta tcatttatgt agcaggaaac tttgctcaac
    47941 aaaatagtga tgtcaataaa atcggcgtat tggctgataa tgagcaaatc agtcagcaaa
    48001 tgagccaagt caaaacagat gattttgagt tcaaagcagt tgattctgaa gaaaaagcac
    48061 aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt agtctcaact gagaatgata
    48121 agataaatgg gatgctttat agcgaaaatt cattaggaca aagtacacaa ctactgatac
    48181 aacaacaact gactggcttg caatcgatgc ttcgagcaag tcaattaggc atttcaccag
    48241 aagaagtagc tagcttgagc caacaagcaa gtttgactcg acaaaaagtc aattttgatt
    48301 caaaaggaaa aatgacagtt ggagaagata actcgacagt ccaatatgtc gtaagttatg
    48361 tagcaacgat tgtcttgttc attattattt tgacgtatgc gcagatcatt gcacaagaga
    48421 tcgcttctga aaaaggcacg cggatcatgg aagtgatcct gtccagtacg actgcacaaa
    48481 agcattttta cggaaaacta acaggtgttt tacttgttgc attaacgcaa atggcattat
    48541 acggtattat ttttggtata ggatacagtc aattcaaaga tatggaaatg gtcaaaaata
    48601 tattagacgg tatctcattg gataacttga ttggaccatt cttgtggtat tctcttctat
    48661 tcatgatctt tggcattttg attttttctg ttttagcagc tttatgtggc tctttggtca
    48721 acaaagcaga agatacagca aaagcaattt tgccagtgac ttatctatct ctaggcggat
    48781 atatgcttgg attgatacta ggagcttcag atccaaacaa tatcgttatt cggatcactt
    48841 cgtatattcc attcttatct tcatatatta tgccggttcg cttagcaaat gagacagtcg
    48901 gtatgggtgg agcattgatt tccttgttgg tacttgtagc cgtaacattc ttattgatga
    48961 tcttgtctgc aaacatgtac aaatcgaatg tactcgtgta tagcgaaggc ggattatgga
    49021 gttcattgaa acaatcgatt tcaattatga gaaatgaacg aaaaaaagca taaaatatag
    49081 aaaaatatag aaggaagctg gacatttatt gttcccagct tcttttttgt atagacctat
    49141 ttctattttt ttattatatt ggatatatat attcattgaa aatagagcat aacaacttta
    49201 taatgttgag tataccaatt tttggaggcg ttttaatgga ccgctattta gatttacttt
    49261 cagagaagtt ccctacaacg gaagatgtca tcacggaaat catcaatctc gaagcaattt
    49321 tgcagcttcc aaaaggaaca gagttatttt tgagcgatat acatggcgaa tttcctgctt
    49381 ttgatcatat tctgcgtatc ggttcaggaa atcttaaaga aaagatcaaa gaccttttct
    49441 ctgaacaaat gacggaaacg gagatcagcc aattcactat ttttacggct tatccagaat
    49501 atgtgttgac gactgattgg tacaaaaaac aagataaatc ccagctgatc catcgtttga
    49561 tcgatctttt acggtttacg acagtcaaat acactcgatc aaaagttcga aaaggattgc
    49621 cgaaagaata tagctatatc attgaggaac tcttgtatat cgatgatcgt atcaacggga
    49681 aaaaagacta tgtaaaaaaa atcattgaac aacttgtctt gatgaaggaa gaagaaaaat
    49741 ttttaacaaa acttgcgcag acgatccaaa aatctgtcat cgatcatttg catatcgtgg
    49801 gtgatatttt tgatcgagga actcaagcag ccaaagtaat ggatcgaatc atggattttt
    49861 cctctgtaga tatccagtgg gggaatcatg atattttgtg gattggtgcg tattgcggct
    49921 ctgaggcatg tttattgacg cttttacgga ttgccgcacg ctacaactat ttatttgaat
    49981 tagagaaaga atatggatta aatttacgtc cattattttc ttttgcccat caaacctatc
    50041 agaagaatcc agtatttacc cctaaaaaca ggaaagatct atctgaaaga gagcaagaag
    50101 aattagaaaa gatccatcaa gcactttcta tcctccaatt caaatcagaa catcagttgt
    50161 tgaatcgacg acctgaattt aaaatggacg atcgtaaatt gttggaaaaa atcaactatg
    50221 aggaatcaac gatcgtttta gaaggtcagc tctatgagtt gaaagggact tgttttcaaa
    50281 cgatccagcc agatgatcct aaaaaattct tggaagagga acaaaaagtg atcgatagtt
    50341 tgatgtattc attccagcac tcgttaagaa tgcaaaaaca catgacgttt ttgatcgaaa
    50401 aagggggaat gtatctcacg aacaatcagc atgtactgtt ccatggctgt atacctgtag
    50461 atgaaaaggg gcagctctta gcatttgaat tagaccaatc ttatcgcgga aaagaactgc
    50521 tgggattttt tgaaaaacag ataactgaaa gcgcaaaaga tttgactgca aatgaagatt
    50581 tggcgaccga tttgatttgg tatgcgtgga gcgggccatt ttcgccttta ttcggcaaga
    50641 gtaaaatggc aacttttgag cgctattttc tcacagagcc tcatacgcac gtcgaaaaaa
    50701 gcaatcccta ttatcacttg cgagatgaag agtggttcag tgaaaaaatt ttggcagaat
    50761 tcgatgcaaa agaagaaggt tcagcaatca tcaatggaca cacgccagta aaagtaaaaa
    50821 aaggagagtc accgatcaaa gcaaacggaa aagcatttgt tattgacgga ggtctatcca
    50881 aagcttatca aaaaacaacc gggattgctg gctactcatt actatgcaat tcttacggtt
    50941 tccaaatcgt gacacattat ccgttcttgc ctattgaaca acaatttgca aaaaaatctg
    51001 atcaaacgaa tgtaaaaaaa gtgatcgagc agcagctccc acgcaaactt aatcggaata
    51061 cgacaaaagg aatggaatta cagcaacaga ttactcaact ccaaaggctg ttggattatc
    51121 gaaaggacga ttgagaaaaa tgctagccaa cattgctcaa tagactaaaa agcgactgca
    51181 acaaaaggcg tgtcgcagtc tccttcggaa ttagctcaaa aaacggtaaa atattagaag
    51241 ctatttttct gtttatttct ctaatactca ggtcagggcg ctgatttcac gacctcttcc
    51301 ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccaa caaacatgag
    51361 aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct cacgacctaa
    51421 tcagaaaaac gattaaaatt cattcacaaa gaaatcgaat aaacgctgtt cagatgaatc
    51481 tacacgatga gaaagttccg gatgccattg gacaccaagg attttgtgca tattatcttt
    51541 actttggact gcttcaatga gtccatcgtt ggaaaaggcg atggctttta acaaaggaga
    51601 aagatctttg actgcctgat gatgatagga attcacaaag tatgaatcag ggagaaggtc
    51661 acttaataaa ctgtccgaaa cgattttgac ttcgtgagtt gcaaactgcg gggcagtggg
    51721 ctgttgttcg tgttttacgg accattttgg atataatgaa agatcttgat aaagtgttcc
    51781 ctccaacacg acattcaata gttgaagtcc tcggcagact gcaaaaatcg gttttctttg
    51841 tttcaatgct tccaagatca atgcctgttc gaattggtct cgttgaatat ttgtttccgt
    51901 cagttttgga tgagggtctt ccatgtaaag ttgcggacta acatcctgac cgcctgcaag
    51961 aatgagtttg tcgattttct caattagttg aggcgctgaa tctggagaca tgactggcag
    52021 aataagaggt aatccttgag catctaaaac tgcatccaca aatccctgcg gtgtatagct
    52081 tacttgattt ccttggaaag tatctgtcgc tcgaatcagt tgattcccgg caatgccgat
    52141 gattggtttc atttttattc cccctaaagt agactaattg taacaaggat ttaaaaaaat
    52201 ttaaactatg taagacttgc ccctatactt atctgttctt gctgtgttac tatctgtaaa
    52261 gagaattttc gtgtgaaatt catgacgata gttgaaaata ttgcttaatt tgttataata
    52321 aaaagtaagc agaaataaaa aggttgggcg taagcttcaa ggatctagtt cttcaagggg
    52381 tgagaaggac attttattga aggggcctgc ctatactaag ttatctttag aggtacatac
    52441 tttcaatgaa aaaagaaagc atgtacctct tttttaaatc gaaaggaagt gaagcaagtg
    52501 ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa atgagaagcg tgctcgaacc
    52561 ctacggcagc agttgaagac ctatgcagaa gaaaaagagc aagaattgga gataatccaa
    52621 acgaacagtc ggcaacagct gaaacagttg gtagaagaaa aagaacaatt cgaaaagcaa
    52681 gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag acaaggtgca agcaactttg
    52741 aaagaccaat atcaaaagca tcagagtcaa gcaatcggtg caatcattga aagggtgaaa
    52801 gaaacttatg gccgtcacta agatggaaaa agtgaccctc atttcagata aaagaaatca
    52861 ggagaggatt ttacaagcag tacaaggaat ccaaaatgtt gaaatccggg atctgtatca
    52921 aaatagaaca aacaaccagt gggtagaaaa atattttcca gataccacga ttattgataa
    52981 agaagcaaaa ttaagcgctt taggcaatcg gctgaaagac atccaagaag ctatccaatt
    53041 catacaacac cacggcgata gccgtcaaaa gaaactccat ttgaaacgtc aagaactttc
    53101 cttacaagac ttggaagcaa attattccga agaaaatttc atgaataaat tagaagaagt
    53161 ccttgattta aaagaaaagt gggaacagct aactgaaaaa cgtgatcgtt tagaagaaga
    53221 ggaagaatgg cttcttcatt ggcaacagct ggatattgct ccaagtatgt atgcaagtaa
    53281 gacgaccatt tttgagatgg ggactgtgag tgcagcaaat tttgaaagtt tcaaggaaga
    53341 actaagtaag cttgatgacg tttatatgga agaaatagat tatacgccaa aacaagttta
    53401 tctttcgtat gttgctttaa aaaaatcagt tgctgctata gaagaaattg ctgggaaata
    53461 cgggttcaat aaagaaacgt atccttatga acaaaatcct aaagaaaaat tgcaagaaac
    53521 caaacagcaa ttatccattg ttcacgagca gcaaaaaaag ataacgacag cgttaggtca
    53581 gtgcagcggc tatatccaag aatttgaatg ggtagaagaa gtcacacttg ccgttgcaga
    53641 acgggaaaaa gtaaaagaac ggtttatcca tgccacttat ctgatcgttt tgcaaggttg
    53701 ggtagatgca gaagaaaaac agaatttgat gcatgtgtta aacgaaactg tatcagaaga
    53761 agatatatac gttagtttcg aaacaccaaa ctcagaagag attcagcagg aagtccctac
    53821 taaattgaaa aaccatccgc ttatcgaacc ttttgaattg ctgactgaaa tgtacagtct
    53881 gccaaaatat gaagaagtag atccaacgcc ttggatgaca ccattttatc ttgtcttctt
    53941 cgggatgatg gtcgcagatg ttggttacgg attgctgatg ctcattggga caatactagc
    54001 tcaaaaacta tttgttttac cccgcgggat gaaacgtttc gtaaaattct ttgagattct
    54061 ctcggtacca tcgatcatct ggggattgat ctacagttca tttttcggac aggcattacc
    54121 aaaagaacta tttggtatcc gcttgccttt cccaatccta tctacgacgg atgacgtcaa
    54181 tacgattttg attttatccg tgattttcgg tctgatacag atcttggttg gattattcgt
    54241 ggcagcaaag gaaaatatca ggaggaaaga ttacttgagt gctgtcagtg atggttttgc
    54301 ctggcaaggg atcctgattg ggatcgttat tgcattgatt ggtgtcatgt tgataaaaaa
    54361 tacagcattt gtttatcttg gcgcggggct atcgatcatt gctgctttat gtatcttaat
    54421 cgtgccgatt atccaatcgc cgtctaaagt caagggtgct gccaaaggag cgtataatct
    54481 ttacggattg actggttata tcggtgattt agtcagttat acacgtttga tggcactagg
    54541 tatttcaggc ggaagtattg gcgcggcatt caatatgcta gttgctttca tgccgccagc
    54601 cgctcgattc agtgtaggga tccttttgat catcgcttta cacgcactga atatgttttt
    54661 gacactgtta agtgcctatg tccatggtgc acgattgcag tatgtcgaat tttttggaaa
    54721 attttataca ggaggcggac gtgcctttga cccgctgaaa acggcggaaa aatacgtcaa
    54781 catcaatcat aagaaaaaaa acaataaata attggaggat ttttatacaa tgatggatta
    54841 cttaattagt caaaatggtg gaatggtatt tgcagtatta gcgatggcaa cagcgacgat
    54901 tttttcaggg attggttcag ctaaaggtgt tggaatgaca ggtgaggcag cagcagcgtt
    54961 aacaacaagt caacccgaaa aattcggaca ggctttgatt ctgcaattgc ttccaggtac
    55021 gcaagggttg tatggattcg ttatcgcctt tttgattttt atcaacttag gaaatgatat
    55081 gtcagttgta caaggattga actttctagg cgcttctctg ccgattgctt ttacaggttt
    55141 gttttcaggt atcgcccaag ggaaagttgc agcagcaggt atccaaattt tagcgaaaaa
    55201 acctgaacat gctacaaaag gaatcatttt tgctgcgatg gttgaaacat atgcgatttt
    55261 aggtttcgtt atttccttcc tattagtatt gaatgcctaa gcataaagga ggaaaagcag
    55321 atgaacgcca ttgaaaaaat catctctcag atgaatgaag cagcagagca agaaagagcg
    55381 gcgttagagc aagaagagcg aatgaagatc gatcaaaact ttgaacaaaa acgtacgcaa
    55441 atcgaaacag aacatcaaaa acaaaaagaa aaacaaatcg aattacttga aaaaaaatac
    55501 cgtcagctta ggaatcgcca acaagtagaa gtacgacaag aaaatttgaa tgcgaagcag
    55561 gaatttttac gacgattgtt tgcagatgca atagctgaaa tggagaattg ggatgaatct
    55621 gaacaaatcc agtttatcaa aaattcattg tattcattac ctttgacagg caaagtaaca
    55681 ttcattgctg gagaaaaatc agcagcttat ctttctcaga cattgctgga tgaatggaac
    55741 aatgaacttc cctttatgat ggttttaagt gatgaaactg ttgctgatca agcgggtttt
    55801 ctgataaacg atcaaggagt tcagtataat tttcttttca gcagccttgt acaagatatc
    55861 caaggaacga tgagttttga gattgccaat caattattcg aataaggagg gatcagtgta
    55921 tggaatacca cgaactaaat cccttgattc gcggaagaga gcttgaactt ttgtccaatg
    55981 agacatttga acggatgatt caaacaaatt ctgtcgaagc acttggtgaa atattgaaat
    56041 ccacgattta cagtccatat atttatgacg gatatgaata tgacttcgaa cagaatcttt
    56101 taaaggagca gagccgtcta tttcgctggt taaaggaatc agcaccggag cctgaaatcg
    56161 tttggatcta tacgatgcgc tatactttcc ataatctgaa ggttttgaca aaagccgaga
    56221 tgacaaatca aaatctggac catttgtata tcgacgatgg attttattca ttagaaacct
    56281 tgaaggatgc cattcatacg caagtttcaa atgaactccc tgaaagcatt atggacagta
    56341 tacgagaagt acgtgattat tttgaagagt ctaccattct tcagggaatt gacgtgattt
    56401 atgatcgaca gtttttgaca gagcagcgac ggcttgccga taagctagga tatcaagaat
    56461 tgctgacaga agtcattgct tttattgact tgaccaatat cacaacgatg gctcgaggaa
    56521 tattgcagaa aagaacatat gggttcatga cgacggtact ttccagctcg ggaagtatcg
    56581 aaaaagaagt atttttgaac tttgtagata aagatatgga agattacacg aattttctat
    56641 tagagacaga ttatgctgat tttctgcgcc ctgtcatccg tgagggaaat cttgatctcg
    56701 taaagcttga acagttgaaa gatgattatc taagttcatt gtatcaagaa gcacaaaccc
    56761 aggctttcgg accactgcca ctactagcct ttttgaacgc aaaagaggta gaaagcaaaa
    56821 accttcgttt actggttatc ggcaaaagaa gccatttcga accagaaaca atcagaaagc
    56881 gggtgagaca agtctatgac gcataagatc ggtgtagttg gcgacaaaga ttctgttttg
    56941 ccattcaaat tgtttggctt tgatgttcgt tatggtacaa ccaagcaaac cgttcgtcag
    57001 gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt atgtcacaga gcaatgtgca
    57061 gcattgagcc cagaaacgat cgaacgttac aaggaacaaa tgatacctgc tatcgtattg
    57121 attcccaatc atcaaggatc attagggata gggctggaag aaattcaaaa aaacgtagaa
    57181 aaagcagtag gtcaaaatat cttatagtat gaaaaggaga tagaaatttt gcaaattgga
    57241 aaaatcataa aagtctccgg acctcttgta atggctgaga acatgtctga tgccagtatc
    57301 caagacatgt gtctagtggg agatctagga gttatcggag aaattatcga gatgcgtggc
    57361 gatgtggcgt ctattcaagt atatgaagag acttctggaa ttggtccagg cgaaccggtt
    57421 cgctctactg gtgaagcttt atctgttgaa ttaggaccag ggatcatttc acaaatgttt
    57481 gacggaatcc aacggccatt agataccttt atggaaatca cacaaagtaa tttcttaggg
    57541 cgcggagttc aattgcccgc attggaccat gagaaaaaat ggtggttcga accaacagta
    57601 gaagccggag aaacggtgag tgctggagat gttatcggga tcgtagaaga aacaaaagtt
    57661 ataaaacata aaatcatggt gccaaatggg ataaaaggaa cgatcaaaac aatcgaaaaa
    57721 ggttcattca ctattgatga aacagtatgt gtcattgaaa ccgaaaatgg tgacaaaaat
    57781 ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc caatcaaaga aaaattaaac
    57841 ccggatgcac ccatgatcac tggacagcga gtgattgata cattcttccc agtgacaaaa
    57901 ggaggagcag cagctgtacc tggtcctttt ggtgcaggga aaacagtcgt tcagcatcaa
    57961 attgcaaaat gggccgatgt ggacatggtt gtctatgtgg gatgtggcga gcgtggaaat
    58021 gagatgacgg atgttttaaa tgaattccct gaattgattg accctaatac aggagaatcg
    58081 ttgatggaac gtacggtatt gattgctaat acatcaaaca tgccagtagc agctcgagaa
    58141 gcttctatct atacgggaat cacaattgcg gaatatttcc gtgacatggg ttatgacgtt
    58201 gctatcatgg cagactccac ttcacgatgg gcagaagcac ttcgtgagat gagcggacgt
    58261 ttggaagaaa tgcctggtga tgaaggttat ccagcttatc ttggttcacg gcttgcggaa
    58321 tactatgaac gatctggaag agtcattgca ttaggatcag aaggtcgcga aggaagtata
    58381 accgcaatca gtgctgtatc cccgtctgga ggggatattt cggaaccagt cacacaaaat
    58441 acattgcggg tcgtaaaagt attctggggt cttgattcca gtttagccca aaaaagacat
    58501 tttccttcga tcgattggat acaaagctat tctctttatt caacagaagt agggaagtat
    58561 atggatcata tccttcaagc tgactgggca tccatggtca ccgagggcat gcgtattttg
    58621 caggaagaac agcagctgaa tgaaatcgtg cgtttggtag ggattgattc cctttcagat
    58681 aatgaccgtc tgacattaga agtggcaaaa tccattcgtg aagattattt gcagcaaaat
    58741 gcttttgatg atgtcgatac attcacttca agagagaaac aattcaaaat gttgaagatg
    58801 atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt taggtgctta tttaagtgag
    58861 atcatggacg gtaccgtatc gattcgtgaa aggatcggac gaagcaaata tatcccggaa
    58921 acagaattag aaaaactaga tcaaattatt ggtgaaatca agcaaacgat gcaaacgatc
    58981 gtttcagaag gaggcatgag caatgattaa agaatatcgt acgatcggtg aagtcgtcgg
    59041 tccattgatg gcggttgaaa aagttgctgg tgtcaaatat gaagaattaa tcgaagtccg
    59101 tatgcaaaat ggcgagattc gtcgtggaca agtattggaa gtccaagaag ataaagcaat
    59161 ggtccaaata ttcgaaggaa caagtggtat caatttacgt gactcttctg tacgattttt
    59221 aggccatcct ttagaactag gtgtatcgga agacatgatc ggtcgagtat tcgatggttt
    59281 gggacgaccg aaagataacg gaccagagat cctgcctgaa aagcatttgg atattaacgg
    59341 agaagtaatc aatccagtag ctcgggatta tccagatgag tttatccaaa caggtatctc
    59401 tgcaatcgat catttgaata cattagttcg tgggcaaaaa ttaccagtat tttctggctc
    59461 tggtcttcct cataaagaat tagctgctca gatcgcgcga caagcaacgg tactcgattc
    59521 atcagatgat ttcgccgtag tctttgctgc tattggaatc acttttgaag aagctgaatt
    59581 tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt tctgtcatgt ttatgaattt
    59641 agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt atggctttga ctgctgcaga
    59701 atatcttgct tatgaaaagg gaatgcatgt tttggttatc atgacagaca tgaccaacta
    59761 tgcagaggca ttgcgagaga tttctgcagc acggcgagaa gttccaggaa gaagaggata
    59821 tcctgggtat ctttacacga acttagctac tttattcgaa cgagcaggaa gaatccgcgg
    59881 actaaaagga tcggttactc aaattccgat tctaacaatg ccagaagacg ataaaactca
    59941 tccaatccct gacttgactg gttatattac agaaggacag atcatcttga ctagagagct
    60001 ttacaagagt gggattcagc caccaattga tgtactcccg tctctttcgc ggttaaaaga
    60061 taagggaact ggacccggaa agacaagaga agaccatgca gctacgatga atcagctttt
    60121 tgcagcctat gcacaaggaa aacaggcaaa agaattagcc gtagttttag gtgaatctgc
    60181 tttatctgat gtagacaaga tctatgcgaa gtttgctgag cggtttgaaa aagaatatgt
    60241 caatcaagga ttctacacga accgttcgat tactgaaaca cttgatctag ggtgggaact
    60301 gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa gacgacatgc tggacaaata
    60361 tctgccagaa gggaagtgag ttctgatggc acgattgaat gtgaatccga cccgcatgga
    60421 attgactcga ttgaaaaaac agttgacaac tgccacaaga gggcataaat tattaaaaga
    60481 taagcaagat gaactgatgc gtcaattcat cctgttgatt cgaaagaaca accagctgcg
    60541 tcaagaaatg gaagagtcta tgacagaagc aatgtctgat tttgtattgg caaatgcttc
    60601 gatcacggaa cctttcatcg aagaattatt tgttttgccg gcggaaaacg ttgatttgtc
    60661 tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg atgaattttg aatatgatga
    60721 acagctgaca gacacacctt tggaatacgg gtatttgaat tccagtgccg aactggatcg
    60781 gtcaatcgat cggtttacta ccctgcttcc aaaacttttg gaactgacag aaatcgaaaa
    60841 aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt cggcgagtca atgcattaga
    60901 atatatgacg atccctcaat tagaagaaac gatctattat attcgtatga agctggaaga
    60961 aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa aatatgggta ccactgacta
    61021 agtggtatct ttttttaaaa gaggaaaacg atgattaaac aaagcaaaca aataaagaaa
    61081 cgtttgccgg ctgttcagct tctcgcatta ggattttttc tcctgatttt agttggcggc
    61141 ggcctactta ccctaccatt ttttagtaat agtgggcatg ggacaaattt tatcgatgcg
    61201 cttttcactg caacttctgc tgtctgtgta acgggattga caacattgaa tacagcagag
    61261 cattggaaca gtgctggtca atttttgatc atgcttttga ttgagatcgg cggtcttggt
    61321 tttatgatgg ttccgattct atgctttgca ttgataaaga agaaagtcag tctcagcaca
    61381 cgtattatat taaaagaagc gctgaatcta gaagaagtat caggtgtgat gaagctcatg
    61441 ctttacatcc taaaattggc ctttgttatc caattgattg gtacggttgc tttatctttt
    61501 gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt acagtttgtt ccatgcaatc
    61561 tctagttttt gtaatgcagg gtttgatttg ttaggagaca gtttagccaa tcaccagcaa
    61621 aatgtttatt tgatcatggt ggtatctgct ttgatcattt ctggtgggtt aggcttcatc
    61681 gtatggcgtg atttactgga atatcatcag aaaaagaaga tgacgattca ttcaaaaatc
    61741 gcattaaccg ttacaggtac ccttttatta ggcggattcg ttgtgttctt tttcacagag
    61801 cgcaatgggg caacgctagt ggaagggaat atgtttcaac gattggcaaa cacctttttt
    61861 atgagtgtga cacctagaac ggctggatat tattcagttg attacctcca tatgagccat
    61921 gcaggtctga ttttaacgat gtttttgatg tacatcggcg gaacatctgg gtcgacggcc
    61981 ggcggattga aaacaactac gttaggtgtg ttgctgatcc agatgcgttc gatgttgaaa
    62041 ggacgtacga gagcggaagc atttggccgt accatccgac aaggcgcagt gttgcgggca
    62101 cttactttat tttttgttac gttgacgttg tgtgtagtcg ccatcatgat tttatcggtg
    62161 actgaaacga tacctaaaac ttctggcatc gaatatattg cttttgaggt cttctctgcg
    62221 tttggtacag taggtcttac gatgggattg acgccagact tgactgcaat agggaaattg
    62281 atcattatag cgcttatgta cattggacga gttggtgtat tgactgtggt cttctctctg
    62341 atgacaaaag cacaaagaac acaatcaaaa tacaaatatc cagaagaaag tgtcatgatt
    62401 ggataatttc atcttctgac agaaacctag tgatttagta aaataagaaa ataaaataat
    62461 ttttcttgct tcttggatac tttaagtgca aaatgttcgt ttattttttg aggcactacc
    62521 cgttgttgaa cctcgggtag tgcctctttt ttattgaaag gaatcaataa gatttgattc
    62581 ctttatttat caataggtta taattcatat ttttttattt tttcttttac gatacttagt
    62641 ccagaagcta aaatttcaag gttattagag gatagggaaa ttcgaacgaa tgcatctggt
    62701 tgcgcacgtg tagtaaagcg tctggaatga taaagtcgga taccgttagc aagaaagtca
    62761 ttctcaatta tcttagggtc aatctcagga tcaactggaa gaattcgata gtaagggaca
    62821 cgattcgttg gtttttctac tttgaaaaga gaatcaaaaa gctgattggc tctttttgta
    62881 agacttaatt tagtttctaa gatcctattt gcttcatttg aatggaggat ttgagtgatg
    62941 acttccacat ctaatccaga agttttcaca tttatattaa aaattgcttg ttctatcttg
    63001 ctttggacat gtactggaac tacaagataa gcaactcgca atccagaaca aataaatttt
    63061 gtcattccag ctaagtagat tgtttggtta ggtaataaat tttggaaagg aggaatgatc
    63121 gtatgttgat agtaagttgt taaaaatgaa tggatatcat cttcaataac ccatagacct
    63181 tctttttcaa tcacttcggc taattgctct cgtcttttgt gagagatttg aaaccctata
    63241 ggattattac aagaaggcat aagaaagaca ccatgaatct tcttttttct acattcctga
    63301 atcaatatat caggtcgcat tccttgctcg tcaaaatcaa ttggaacgat ctctaaatga
    63361 tataactgag ccaattcaat gaaattagcg tatgtatatc ggtctgttgc tattctatca
    63421 cctggagaga aaagtgcaga aagtgtgata gccaatccat tttgaacacc ggaaacaata
    63481 gatagagatt ctaaggacgg aatgactcct tgagttttta accaatttgc tgcagtagtc
    63541 aattgaaaat ttgtgccttt gggatcttgg taattaagca aaccaacaag atttgggtta
    63601 ttactagttg attgaataaa cgggacaacg aatttattac attcttcaaa agaactaacc
    63661 agaccaaaat caatgatatg ttcggttact tcattggtag agatggtgat ggactcaaag
    63721 acattgggag aaataaatgt gccacttcca atattcgtat acagcaaacc tttctcaatt
    63781 ccatatttat aagccttgcc aacggtagta aaatttaaat ctaaataatc agctagctct
    63841 ctttgggagg gtaactgagt gtttttctgt aatttattct ttaaaatatc ttgttctaat
    63901 tgttgaatca acgatcgata ataaggacgc ttcaattttt gtttgtctgg tcgccaagtt
    63961 aacgggtaat tgtcataaga attaataggc attttctcca ctccttaaaa ttgtatacca
    64021 tacaatttta ccattgattg tattgatttt agaagttaat cttttgtgag gagggaattg
    64081 tctatgaaaa ttattaattt tgaggaagta gtagcttctt ttagttttga agaagcgatt
    64141 caagtaatga aaaaatgttt ttttgattat gaaagtggta aaatcagcca aaatcctcga
    64201 ttggtggaaa ttttacctga cggaaaaggc aaaaatatgt ttgcgatgat gcctgcttat
    64261 ttgggggaga acagatattt tggcacaaaa gtcattaccg cttttccgga taatcatcag
    64321 tatcatttac cttctcattt aggagagatt ttcttgtttg atgctcaaaa cggactaccc
    64381 aaagcattga taaacgcaaa tgcagtcacc tggattcgaa cagctgctgt atctgcgctg
    64441 gcaacagatt acttagcaaa tccgcatgca gaaacacttg ctttaattgg agctggtcaa
    64501 caagcattgt cgcatttgac tgcgatttct accattcgtc ccatcactac agtgtttgtt
    64561 tacgatattt tagaagaaag acgaaatact tttatcgcta aagctaaaaa acagtatcct
    64621 aaaataacgt ttataaatgc aatatcagta gaagatgcag tacgtccagc ggaaatcatt
    64681 tgtacgttga cctctagtaa agaggcattt cttcaaaaag agtgggtaag ggaagacgca
    64741 catatcaatg ctgtgggcac atttactcct gatacaagag agataagtac atcgttgatg
    64801 gaaacttctc aaatctatgt agatgattac tcagctgcct taacagaaag cggggacctg
    64861 cttatagcca tggcagaagg agatttagag gaatcttgta ttgttggtac attaggcgaa
    64921 ttagtttctg agaaaaaaac attttataaa ggagaatcga ggccgacgat tttcgacgca
    64981 gttggtctag ctattgaaga tctttgttgt gcagaatata tttataacaa attagagaaa
    65041 ggggctttaa aatgaactta gattattacg tagttgatgc atttgcagat gaggtgttca
    65101 aaggaaatcc agcagcggtt tatgtgctag aagaatggct gccagaaggt acgatgcaaa
    65161 aaattgccat tgaaaacaat ttatctgaaa cagcatttac agtgaagaaa aaccaagaat
    65221 ttgagcttag atggtttacg ccagatcgag aaatcgatct ttgtggtcac gcgacattag
    65281 caacagcatt tgttttattt aactattaca aaatacctga tgaaacgatt aaattttcta
    65341 ctcaaagcgg taatctattt gtgacaagaa ttaaagatta ttattacatg gattttccta
    65401 gtattatgcc gaaaaaagtg ccgatattgt ctgaatatga agaagcgatt ggggctaaga
    65461 ttaaagaagc ctacttagca cgtgatttat ttttcgtatt agaagatgaa aaaacggtgg
    65521 ctaaattgca gccagatttt acagctatca aaaattttga attaggaata ggagtcatcg
    65581 tgacagcaga aagtgtgcaa aaagactttg tttctagaac cttctttcca aaattaacaa
    65641 tcaatgaaga tccagtatgt ggatcagcac attctaatct tattccttat tgggcagaaa
    65701 gaatgaataa aacaaaattg tcagcttatc aaatgtcatc aaggggcggg gatctagaat
    65761 gtgaattaaa agaagatcgt gtgataatag gaggaaaagc agttttgttt tctaaaggta
    65821 cctcgtttat ttgatttctt tcaaatgaat taaaaataaa ttggtggaga gtggcaacct
    65881 tatagctaca atggttgtta aaaaaagacg caaacttttt caatttaatg aaaagtttgc
    65941 gtctcatttt aattattggt gtttgttttt tattcaatca agagtaaaat gccccatact
    66001 aactatcctc ggatgacttc gattttataa ccatcagggt caatgacaaa gtagtaagaa
    66061 ggcgcagtgc caggtaatcc ttttaagtca gtaatctcaa gaccggcagc tttatgttgc
    66121 tcgtgtaatt tttctagatc atcagctgct atagcgatat gaccatagcc gtctcctaga
    66181 tcatacgcac catgatcata gttgtaagtc aactctaatt catagtcatc cccaggaagt
    66241 gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac ggcggcggct ttcttcaaaa
    66301 ccaaatgcct tttgataaaa ttcgattgat gcttcaagat ctttcactcg aacacatgtg
    66361 tgagccattt tcatagattc atctctcctt ttccttttac tcaatcataa caaaaagaag
    66421 taaggaaata gttgatttgt gcttgttcat gagaaatttc tcatgtaaaa tggatagaaa
    66481 ataaaactgt taaggtggaa actattcatg gataaaccaa tttttggtga aaaaaagcaa
    66541 tttgctgatt atcaaacccg ttatgcagca tatattgtca tagaaagaga tcagcagatt
    66601 gcagtaatcg aggctccaaa tggtgctttt tttcttcctg gcggggaaat tgaaggagaa
    66661 gagacgaaag aagaagcgat tgtccgtgag ctgatagaag aaatgggaat tgtggcagaa
    66721 atttcttctt atctgggaga agctgatgag tatttttact cgaattatcg tgcaacttat
    66781 tattataatc caggatattt ctttgttgct gattcttgga agcgtatagg agaaccaact
    66841 gaaaagacca ataaaatttg gtgggtgagc cctcaagaag cgcttgtgaa attgaaacgt
    66901 ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa ataaaaactg aaagaaagcg
    66961 agtagatcag ctttctctca gtttttttat ttattacagt acatattttt tgattgcttc
    67021 agctacaccg tgctcgtcgt ttgatgaagt gatgacattt gctgcttctt tgacaagaga
    67081 aacggcattt cccattgcta ctcctagacc agcatattca atcatggata agtcgttctc
    67141 gttatctccg atagccatga tttcatcttg atcaatacca aggtgctgtg cgagattcgc
    67201 aactgccgca cctttacttg cttctttatt tagtacttcg aagtaatagg gcgcactttt
    67261 gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc gcaattgctg catctaaaat
    67321 ttctggttcg tcgatcatca tcattttgac gatgttcatg tctggtgtca tttcttcagg
    67381 tgttcgatat tttagcggca tcttgactaa acttgcttcg tggatcgtat aagcgctgat
    67441 atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa tggagatgac tgccgacacg
    67501 gcgtgccatc acttcgatct ctaggaaatc ttcatgtgtc agtccgtgac gggcgatgat
    67561 tttacctgat ttcgtttgtt gtacgagtgc cccgttataa gtaataacgt aatcattatc
    67621 ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct ggtaagggac ggccagtgca
    67681 taaaacgatc gaaacacctt ttgcaacagc agcggcaatc gcttctttta cttcttttgt
    67741 tacttctctt ttctcattta ataaagtacc atcgatgtcg atagcgacta gtttgataga
    67801 cataatttcc tccaatatat tttaatttat ttcaatcaat gcgccattac ggatatggct
    67861 ggcaaattct tgataagttt cgtcaaataa atcataatga tcacgcaagg acggatcaac
    67921 catttctttt ggaaagaaaa atcgctcatc tccttgttct tgtcccgcta atgcagcaac
    67981 aagctgacta acctgtgata attctatcaa agttccgtca tttcgcaaaa tctcgatttg
    68041 tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa ggtagatcgt agctagaatt
    68101 cacagcagtg taataaacag gattatatcc gaccttttct actaacaaag tgagttcttg
    68161 gactaatttt gaatcccggt tgccagaaaa agtagcagat ttcagtggtt tacgattcaa
    68221 aaaacgttta gctaaatcat ttaaaatagg atcagcagat tcagaccatt gagtaaagta
    68281 agtgcccaag accccatcat caagtttgag gtattcttct aaagtaaagc ttccgtcaag
    68341 aaatggcaga agtaattgag aatggagttc aaaactgctg gcatcttctt gatacaactc
    68401 gtgtgcgcga tgaagcagat gctccaaaat tacttccata ccacgggaaa caggatggaa
    68461 gtacacttgc acatacattt gatatcggct aacgatataa tcttccactg catgcatgcc
    68521 actcatggaa aaagcgatcc cgccttcata aggacgaatc acacgtaata ttcgggtaag
    68581 atcaaatgtt ccatattctg ttccagtgaa ataagcatcc cgaagcaaat agtccattcg
    68641 atctgcatcg atctgactag aaatcatttg gacaacttgt gggttggggt aagttttttg
    68701 gatcacacta gctacctttt caggaaaacc ttcttctact cggttaagaa tctgatacac
    68761 ttctgtttct ggagaagtga tgatctctac agtgatggct tcatgattcg tatggaagat
    68821 atgttcaaat gtatgagagt aaggaccatg accgacatca tgcaacagtg ccgcacataa
    68881 agtcaccagc cgttcctgat catcccagcc tccgttgcct attttttctt ttgaaaaatt
    68941 tcgagaaaag atatcgcaga ttcgtcttga aatctcataa acgcctaatg aatgagtaaa
    69001 acggctatgt tctgctccgt gaaaagtgaa agaagaagtg cctaattgtt taatgcgtcg
    69061 tagacgttgg acttctttcg aattgatcaa atctaagatt acttgatgct ggacatggat
    69121 atagttatgg actggatcac gaaaaacttt ttccataggc agcatttgat atttataagg
    69181 ggttgtcata atgtagctcc tttatgttat tacgttgttc cattctctgg atttggttgt
    69241 tccattgctc cagctggatt tcttttttta gccagtcggt agaatcctgt aaatcacttg
    69301 cagaatacag cttttcaaaa gcttttgcaa atgcttcttt gacttctttt acaaaaaggg
    69361 gttggtcaag cagttcttca agtgttgcca ttgtagcagg atccactgca ggatagccat
    69421 ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg cttcatgact tgcccgcgtt
    69481 tttcctgatt accgttgaca cttaagtaca tcataacaga aatcccattt ttgatccgac
    69541 gctgagcgat accggcaaat tttttgccat ggatgcttaa atcataagtt ccaggacaat
    69601 aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg aaaagcgtat ttagtcaata
    69661 ggagcatttg ctcgtaagcg tcatctatgg ttattttttt tgtgtcactt tgggggaaaa
    69721 tcaaagaaat attcaaaatt ccttcatctg atacgatccc taatccacct gaatttctga
    69781 tcactggagc gtaacctgca tctttcagtg tttggatccc gtcacttaga aaaggagttc
    69841 gaacatcctt catacctaag atgactgtgc agtcatattg ccagaaatgt aaaaagattt
    69901 gctggtgttc tttagaatat tccgttaata tatctgttaa tgcaaaaggg gaaaagtcgt
    69961 ttttttgata aatgtgctga tctagtcgaa tcgcagggtt cattcaaccg gctcctttgt
    70021 ataaattcat attttctaca atattatact ctaaaatcaa agagttgttt tggaaaaaag
    70081 gttttctgta aaaatggaca agttggttga caaaacaaaa gaaactagga acaatgtagc
    70141 tatgagaagt aaaggagaat gaataatgga attatcacaa ggaacaccga tttcaatcaa
    70201 attgcgtaca aaagtaaaac agcaagggga agtgcaagat ttttattttg atttgaaagg
    70261 gcaaatggtg acaattggcg atacgctgta catacgttat aaagagattc aggaggagac
    70321 gggcgatgag attcctgtaa cgatcaaatt agttccagat ggtcatgtac agctgatccg
    70381 tgcaggagaa atgcggatgc gtttgaaatt tggctataaa gaacggttag aaacatccta
    70441 tcgaacacca tacggtatga tccagattgc tacttttacg aaagaactgc atgttagttt
    70501 gaaagaccgt cccacaggag ggaaggttcg aatcgattat gatctgttca tgggaccgga
    70561 aaaaatcggc gaatattatt tgaccttgga ttttacagcg taaatataaa agaaaagttt
    70621 gattttttgt gtctgatttt gtatgataag gagtagactg tgaaaggacg tgtcctgatt
    70681 ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta tcaatgatcg aagtagcaca
    70741 cgcgatctta gaacaacgtg gcgatgtcat ggatttttct gatttagcta accagatcca
    70801 aaactatctt gggaaatcag acagtgaaat tcgtgattca ctcgcacaat tttatactga
    70861 cttgaatatt gacggcagct ttatctcatt aggggataat cgttggggct tgcgttcatg
    70921 gtatgctatc gattcgatcg atgaagaagt aaaccatgga atggacgaag aggatgaaga
    70981 tactccacgt cgccgtaaac gcaagaaagt caacgccttt atcaatgatg atgaagacgc
    71041 gatcgattac aatgacgatg acccagaaga tacagatctg acagaagaag atgacgatga
    71101 tctgtttgat gacgatgatg atgaagatga agaaatcgct gcttataatt ctgacttgca
    71161 agaaatcgga gcagatgacc cgacagatga tgaagaagac ttgccaggaa atatcgaaga
    71221 agatttaagt atcatagacg aagacgatga tgatgaagat tatgaagagg aagaattttc
    71281 tgactctgaa aaagaataaa cattattttc ttacgagagg ttgtgacaga gacgatcagc
    71341 ctatcagaat aaggaggtgt gacacgattt ttgtcacacc ttcttttttg tatataaaaa
    71401 tcccaagtaa atacataaaa aataaaaaaa ttgaaacttt tagcgcacct tgtacgtcat
    71461 attaagtgag gcagaaagga gtggagaatg atttttactg gatataagaa aaaacggcag
    71521 atcgaaaaat accgtcacct gctgaatgga ctcatcgaaa ctgattatca aaaaatgttc
    71581 cgtatcacga tgaattacgt acatgataaa gaagaggcct tagatgtgat gcaagatagt
    71641 tttcataaag ccctgaacta ttttgaaaaa gaaaaagaga tcgagcattt tagtgcatgg
    71701 ttttatcgga tattgatccg cacggcatta gatagttggc gcagacaaaa aaggacgcct
    71761 gtggacttat ttgaaattca agatatcccc caacagttac ctttagaaac ttccgttgca
    71821 gagctccata tgattttaga taaaatagac agtccggaaa aagagattct gatcctctac
    71881 ttttttgaag gatttcgttt gaaagaaatt gcgactatct tggatatgaa tgaaaacaca
    71941 gttaaaacga aaatgtaccg ctcattgaat gctttacgtc aaatcctaga atgaaggaga
    72001 gaagagtgcc atgtattccc aaaaagaaaa tttaaaaaaa ctaaagaaaa cttataaaga
    72061 acaagcaatt cccaaagggc ttaaagagga gatccgccag cgttttatta tcgaagagca
    72121 atcatttata aaagcaaagc ggagaaagag acgtctccaa gggttcggag tatctttggc
    72181 tatcgtaatg attacaacaa gtagccttct ttttaatagt caagttcgaa gctttgcaga
    72241 agatttaccc atcttaggat cagtcattga attgatctta ggcgaacgct ttactgatcg
    72301 atctgaaaaa atcgatattc aggtaccgaa gatcaataca caaaatgaga aagaaaataa
    72361 aacaattcat ggattaaatc aaaaatactt tcgcgaagga caagcagatt ttgaaaaagc
    72421 tgaaaaagaa tatggaaatt ttgaaacgga tcattaccaa gttttgggag attatcagaa
    72481 aatcgtagac gataaccggt ttctcgtgat tgaaaggcag atcacccaaa cagcagcaga
    72541 tagccatgtt gaaaaaagat acgacacaat cgacaagaaa aacagtgtcc agctctcact
    72601 tccactttta ttcaaagatg acacctatct atctgttttg acaaaagaag tcaaacggca
    72661 gatggccgaa caagtcaagg aagatccatc taagtactat tggactgagc aagatattca
    72721 agaagggacg atagaaaaac caacacttgt tacgcctaca cgaagttttt atttgaacaa
    72781 agagcatcaa ttagttttga ctttttctca atacgaaatt gctcctggtt atatgggtac
    72841 accagaattt gtcattccta agtccgtgac aaaaacgatt ttggcatcgg aagattattt
    72901 ggatcattag acgagaagta gttgcgacta gcagtttcaa ttttcaagta agtacaagta
    72961 aataataaga agagaaaaag agaaagaaga gatatagtga aaagaaatta tttagttatc
    73021 agtattattg ctttatccgt gcttttgttt ttcgaaatta cttcggtggc ctccttgatc
    73081 accgaagtaa aaggacaaac acactcttcg gagcattcgt cacaatcttt caaacaatca
    73141 gaacaatcaa ccattaaaag ccaatcaact gaaacagcta acgtcaaaga agccattcaa
    73201 gaatcagcgg aaaaggtaca aagtcagtca tcacaatcaa cacaagacag tacaaaaacg
    73261 agccgagatc cggctgtcaa aagcgtcgca atcagctttg atgatggacc aggagcgacc
    73321 acaacaccgc agcttctgcg aatcttaaaa gaaaaaaacg tgcatgccac ttttttcgtc
    73381 ttaggagaaa atacggcgca gcatcctgaa atcgtgaagc agacagctga agcagggcac
    73441 gagattggca atcacacata cgatcatcag aatctggcca ctctttctgc ccagtcgatg
    73501 acggaggaag tgacgaaagc agacactgag atcaaaaaag tcactggcaa aacccccact
    73561 tttgttcgtc cgccctatgg ttctgtcacg aatgtaggag caacgatcat ccagcgaccg
    73621 atcatcgaat ggtctgttga ttcagaagat tggaagacaa gaaatcctga tttgattttg
    73681 cagaagatcc aagcaacagt atacgatggg gcaattattt tattccatga tatctatcct
    73741 gaaacgattc gtgccgttcc tcaagtgatc gattatctga aagagcaagg gtatcgcatc
    73801 acgacagttg gggatttatt agggcatcca acggctgtcg agaactatta tggcagaaac
    73861 gatcatcggc cagttcagta agtgagacaa gtatctttaa taaataacac attttaataa
    73921 gtagttgaac acataactac cttacttgca aaggcttaac aatatctata caggtacaac
    73981 aaaaaactgt ccaccaattt tctttggcgg acagttttca cttaaaaata aagaagcaac
    74041 tattcgtaaa ttatgctaaa atagcagtag aacgcgtcct gagggaatcg aacccccgtc
    74101 tcaagaaccg gaatcttacg tgatatccac tacactaagg acgcaagcac tgggataatt
    74161 gtaactaaac agataaaaaa ttacaagggg attggacaaa aaaatgaaat ttgagcaaaa
    74221 tttttcacaa aaacagcagc agacacagaa actagccatg acgcaacagt tgcaacagtc
    74281 catccaagtt ttacaatata acagtgagga gctgttggca ttcgttgaga atcaagcaat
    74341 ggaaaatcca ttggtggaag ttgttgagcc ggaatggcag ccagattaca taaaagcttc
    74401 ttcatcttct tatgaaggag aagagacaaa ttacctaaat cagattcctg atacaaaagg
    74461 gtctttattt gattcattga tcgaacaagt ccatttgaat taccgagata cttttttgag
    74521 gaaaatcgtt ctttatcttg tagaatacat cgatttgaac ggatttttga cgattagttt
    74581 agaagaagcc ggaaaagaga ttggagctac gcctatccaa atgctcgatg cgttgactct
    74641 gattcagcaa ttagaaccag ctggagtagg tgcacgtagt ttgcaagaat gtctcatgct
    74701 tcaaacagaa cgagatgatt atgcgccgga attagcttat atcgtgttgg aagaatgttt
    74761 tgaggaactg gtggagcgaa aatggaaaga aatcgcacaa cgtttttctg ttgatctaca
    74821 tgctgttcag cagatattcg actatttaca aacgttgagt ccaagccctg gccggatttt
    74881 cgaccgtagc agcgaattgt acattattcc tgatgtccgg gtattagtgg acgacgataa
    74941 aaatgtacaa gttatctcaa accgaaaaaa tcagccaaat atacgctttc aagagagtta
    75001 cttcaaacaa atgtcacaac aagcagataa agaaactgaa aattatttaa aagaacggaa
    75061 acaggaattc gaatggttga agaaaacgat cctacaacgt ggggatacaa ttttgcgggt
    75121 tgctcaagtc attgtttccc ggcaaaaagc tttctttatc gacaaggaac gtccaatcaa
    75181 gcctcttacg ttaaaggaag ttgcaactga gatagatgtg catgaatcca cggtcagtcg
    75241 tgcagtcaac gggaaatatt tggaaacaga cttcggtgta tttgaattaa aaaaattttt
    75301 cactacacga attcctacga atagtacaga acagacagaa gacctttcag cagatacagc
    75361 gaagaaaaag ctgcaggaat tagtcgatca agaagacaaa aacaagccgt tgtctgacca
    75421 gaaattggtt gaattgctga aaaaagatga gatagcgatc tcaagacgaa cagtagcaaa
    75481 atatcgtgat ttattaggaa tcccgagttc atcaaaaaga aaacgttatg ataactaatc
    75541 agtcaaaaga ctagaaaaag tatttcactt tttctagtct ttttagttga aaaacgagag
    75601 caaaactgtt agacttacag tcgtgaggtt gagatttccc tttattgatg tgaaattcga
    75661 atagttaatt tcttttttta tcgtatatgg gtcgttttaa gtctacattg gacgtaaagt
    75721 gtccaactaa ggagatcgct atgctggatg aaataaaaat gattgaatct gttgcaccag
    75781 atattatcga agtgttgcaa gaacgctata aaattttacg aaatatctat tggatgcagc
    75841 cgattgggcg tcgaagttta tcggaaagta tggggatcac agaacgagtc ttacgaacag
    75901 aaacagacct tttgaaaaat ctgaatttga tcgacacctc aaaaagcggt atgacgctga
    75961 caactaaggg cgaagaagtt tatcaaagcc ttgaaaactt catggatcag cttttaggta
    76021 cgcatcaaac cgaacaacag ttggcagaat attttggtaa ccaacgctgt atcgttgttt
    76081 caggaaacag cgaagagcag accaaagtgg ctgatgcatt cggcgaagcg ctgagcgaag
    76141 ccttagaccg actacttcca gaaggcgaga atatcatcgc tgtgatggga ggaactacta
    76201 tggctgtggt agccgaacaa ttgtctaatc tggaaaataa gaaacggcat aacctgtttg
    76261 tccctgcaag aggcgggatt ggtgaagcga taactgtaca ggcaaattct gtcagtgcaa
    76321 gaatggctgc taaagcaaac ggtaaccacc gcgcacttta tgttccggaa caattaagtc
    76381 tggcaactta caattcttta ctcaatgagc catctatcca agaagtctta aacttgatta
    76441 gtgaagcgaa ttgtgttatt catagtattg ggcgtgcatt acacatggca gcacgtcgta
    76501 agatgactga aaaagaatta gtcatgctga aacaagcaaa tgctgttgcg gaatcttttg
    76561 gctacttttt caatgaaaaa ggtgaagtcg tttataaggt ccctagaatc ggcatagaat
    76621 tgcaagattt ggaaaaagtc ccgatcatca tggcaatcgc gggcggaaaa tcaaaagcta
    76681 aagcaattcg agcttatatg aaaaatgcac caaaacaaac gtggctcatc actgatgaag
    76741 ctgctgcaaa tgagatttta aaaggggtaa ccctttaaaa taaatatttt tttgattttc
    76801 ataaggagga aatcttaaat gacagttaaa gtaggtatta atggttttgg acgtattgga
    76861 cgtctagcat tccgtcgtat ccaagatgta gatggaatcg aagtagtagc aatcaacgac
    76921 ttgacagatg ctaaaatgtt ggcacacttg ttaaaatacg acacaactca aggacgtttc
    76981 aacggaacag ttgaagttca tgaaggttca ttcaacgtaa acggaaaaga agttaaagta
    77041 ttagctaacc gcaacccaga agaattacca tggggcgaac taggcgtaga tatcgttcta
    77101 gaatgtactg gtttcttcac ttctaaatca gcagctgaaa aacatttaac agctggtgct
    77161 aaacgcgttg ttatctctgc tcctggcgga aacgatgttc caacaatcgt ttataacaca
    77221 aaccacgaaa cattaactgg taaagaaaca gttatctcag gtgcttcatg tactactaac
    77281 tgtttagctc ctatggctaa aacattgaac gacaaatttg gtgttgttga aggattaatg
    77341 acaactatcc acgcttacac aggtgaccaa atgactctag acggacctca tcctaaaggt
    77401 gacttccgcc gtgcacgcgc tgctgctgca aacatcgttc ctaactcaac aggtgctgct
    77461 aaagctatcg gtttagtaat tcccgaattg aacggtaaat tagacggagc tgctcaacgt
    77521 gttcctgtac caacaggttc attaacagaa ttagtaacag ttcttgaaaa agaagtaact
    77581 gttgacgaaa tcaatgcagc aatgaaagaa gcttcaaacg aatcttacgg atacaacaca
    77641 gacgaaatcg tttcttcaga tatcgttggt atgacttacg gttcattatt tgatgctaca
    77701 caaactaaag taatgacagt tggcgacaaa caattagtta aaactgttgc ttggtacgac
    77761 aacgaaatgt catacactgc acaattagta cgtactttag aatacttcgc taacttataa
    77821 gatagacaaa cgtacaagtg taaattatag acatgtacat ttaaaagcgg ggaagcaatc
    77881 gcgcttcttc cgcttttttt gataataagt ttatt
//
LOCUS       DACDIP010000007        76355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDIP010000007 DACDIP010000000
VERSION     DACDIP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.007"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..467)
                     /locus_tag="IV257_000544"
                     /pseudo
     CDS             complement(1..467)
                     /locus_tag="IV257_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            complement(670..1149)
                     /gene="rlmH"
                     /locus_tag="IV257_000545"
     CDS             complement(670..1149)
                     /gene="rlmH"
                     /locus_tag="IV257_000545"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8813697.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1577..2866
                     /locus_tag="IV257_000546"
     CDS             1577..2866
                     /locus_tag="IV257_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8813698.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2905..3510)
                     /locus_tag="IV257_000547"
     CDS             complement(2905..3510)
                     /locus_tag="IV257_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8813699.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3715..4179)
                     /locus_tag="IV257_000548"
     CDS             complement(3715..4179)
                     /locus_tag="IV257_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8813700.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4201..4521)
                     /locus_tag="IV257_000549"
     CDS             complement(4201..4521)
                     /locus_tag="IV257_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8813701.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4665..5744)
                     /gene="pepA"
                     /locus_tag="IV257_000550"
     CDS             complement(4665..5744)
                     /gene="pepA"
                     /locus_tag="IV257_000550"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8813702.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5944..6294
                     /locus_tag="IV257_000551"
     CDS             5944..6294
                     /locus_tag="IV257_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8813703.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6517..7464
                     /locus_tag="IV257_000552"
     CDS             6517..7464
                     /locus_tag="IV257_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8813704.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7521..8939)
                     /gene="pepV"
                     /locus_tag="IV257_000553"
     CDS             complement(7521..8939)
                     /gene="pepV"
                     /locus_tag="IV257_000553"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8813705.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            9088..9928
                     /locus_tag="IV257_000554"
                     /pseudo
     CDS             9088..9928
                     /locus_tag="IV257_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
     gene            9932..10372
                     /locus_tag="IV257_000555"
     CDS             9932..10372
                     /locus_tag="IV257_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8813706.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10422..10616
                     /locus_tag="IV257_000556"
     CDS             10422..10616
                     /locus_tag="IV257_000556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813707.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10752..11438
                     /locus_tag="IV257_000557"
     CDS             10752..11438
                     /locus_tag="IV257_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8813708.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(11846..12535)
                     /locus_tag="IV257_000558"
     CDS             complement(11846..12535)
                     /locus_tag="IV257_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813709.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12510..14639)
                     /locus_tag="IV257_000559"
     CDS             complement(12510..14639)
                     /locus_tag="IV257_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8813710.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14687..16024)
                     /locus_tag="IV257_000560"
     CDS             complement(14687..16024)
                     /locus_tag="IV257_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8813711.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(16276..16635)
                     /gene="rplT"
                     /locus_tag="IV257_000561"
     CDS             complement(16276..16635)
                     /gene="rplT"
                     /locus_tag="IV257_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8813712.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16688..16888)
                     /gene="rpmI"
                     /locus_tag="IV257_000562"
     CDS             complement(16688..16888)
                     /gene="rpmI"
                     /locus_tag="IV257_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8813713.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16960..17484)
                     /gene="infC"
                     /locus_tag="IV257_000563"
     CDS             complement(16960..17484)
                     /gene="infC"
                     /locus_tag="IV257_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8813714.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            17804..18436
                     /locus_tag="IV257_000564"
     CDS             17804..18436
                     /locus_tag="IV257_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8813715.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(18438..19343)
                     /locus_tag="IV257_000565"
     CDS             complement(18438..19343)
                     /locus_tag="IV257_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8813716.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(19428..20081)
                     /locus_tag="IV257_000566"
     CDS             complement(19428..20081)
                     /locus_tag="IV257_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8813717.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            20403..21347
                     /gene="mvk"
                     /locus_tag="IV257_000567"
     CDS             20403..21347
                     /gene="mvk"
                     /locus_tag="IV257_000567"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8813718.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            21340..22317
                     /gene="mvaD"
                     /locus_tag="IV257_000568"
     CDS             21340..22317
                     /gene="mvaD"
                     /locus_tag="IV257_000568"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8813719.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNFKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            22330..23415
                     /locus_tag="IV257_000569"
     CDS             22330..23415
                     /locus_tag="IV257_000569"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8813720.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            23412..24476
                     /locus_tag="IV257_000570"
     CDS             23412..24476
                     /locus_tag="IV257_000570"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8813721.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24725..25528)
                     /locus_tag="IV257_000571"
     CDS             complement(24725..25528)
                     /locus_tag="IV257_000571"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8813722.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25548..26390)
                     /gene="kduI"
                     /locus_tag="IV257_000572"
     CDS             complement(25548..26390)
                     /gene="kduI"
                     /locus_tag="IV257_000572"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8813723.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(26406..27413)
                     /locus_tag="IV257_000573"
     CDS             complement(26406..27413)
                     /locus_tag="IV257_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8813724.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(27420..28064)
                     /locus_tag="IV257_000574"
     CDS             complement(27420..28064)
                     /locus_tag="IV257_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8813725.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(28164..28922)
                     /locus_tag="IV257_000575"
     CDS             complement(28164..28922)
                     /locus_tag="IV257_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8813726.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(29049..30200)
                     /gene="fucO"
                     /locus_tag="IV257_000576"
     CDS             complement(29049..30200)
                     /gene="fucO"
                     /locus_tag="IV257_000576"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8813727.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(30282..30593)
                     /gene="rhaM"
                     /locus_tag="IV257_000577"
     CDS             complement(30282..30593)
                     /gene="rhaM"
                     /locus_tag="IV257_000577"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8813728.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(30595..31440)
                     /gene="rhaD"
                     /locus_tag="IV257_000578"
     CDS             complement(30595..31440)
                     /gene="rhaD"
                     /locus_tag="IV257_000578"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8813729.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(31454..32710)
                     /gene="rhaA"
                     /locus_tag="IV257_000579"
     CDS             complement(31454..32710)
                     /gene="rhaA"
                     /locus_tag="IV257_000579"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8813730.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32707..34167)
                     /gene="rhaB"
                     /locus_tag="IV257_000580"
     CDS             complement(32707..34167)
                     /gene="rhaB"
                     /locus_tag="IV257_000580"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8813731.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            34320..35189
                     /locus_tag="IV257_000581"
     CDS             34320..35189
                     /locus_tag="IV257_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8813732.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(35315..36214)
                     /locus_tag="IV257_000582"
     CDS             complement(35315..36214)
                     /locus_tag="IV257_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8813733.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(36379..37203)
                     /locus_tag="IV257_000583"
     CDS             complement(36379..37203)
                     /locus_tag="IV257_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8813734.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(37214..38509)
                     /locus_tag="IV257_000584"
     CDS             complement(37214..38509)
                     /locus_tag="IV257_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8813735.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(38525..39004)
                     /locus_tag="IV257_000585"
     CDS             complement(38525..39004)
                     /locus_tag="IV257_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8813736.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(39010..40038)
                     /locus_tag="IV257_000586"
     CDS             complement(39010..40038)
                     /locus_tag="IV257_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8813737.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(40055..41161)
                     /locus_tag="IV257_000587"
     CDS             complement(40055..41161)
                     /locus_tag="IV257_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8813738.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(41390..43987)
                     /gene="adhE"
                     /locus_tag="IV257_000588"
                     /gene_synonym="adhC"
     CDS             complement(41390..43987)
                     /gene="adhE"
                     /locus_tag="IV257_000588"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8813739.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(44292..44672)
                     /gene="yajC"
                     /locus_tag="IV257_000589"
     CDS             complement(44292..44672)
                     /gene="yajC"
                     /locus_tag="IV257_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8813740.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(44769..45914)
                     /gene="tgt"
                     /locus_tag="IV257_000590"
     CDS             complement(44769..45914)
                     /gene="tgt"
                     /locus_tag="IV257_000590"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8813741.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            46132..46830
                     /locus_tag="IV257_000591"
     CDS             46132..46830
                     /locus_tag="IV257_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8813742.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(46998..49637)
                     /locus_tag="IV257_000592"
     CDS             complement(46998..49637)
                     /locus_tag="IV257_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8813743.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(50256..51287)
                     /gene="queA"
                     /locus_tag="IV257_000593"
     CDS             complement(50256..51287)
                     /gene="queA"
                     /locus_tag="IV257_000593"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8813744.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            51480..52655
                     /locus_tag="IV257_000594"
     CDS             51480..52655
                     /locus_tag="IV257_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8813745.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            52659..53300
                     /locus_tag="IV257_000595"
     CDS             52659..53300
                     /locus_tag="IV257_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813746.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            53297..54214
                     /locus_tag="IV257_000596"
     CDS             53297..54214
                     /locus_tag="IV257_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8813747.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            54211..54882
                     /locus_tag="IV257_000597"
     CDS             54211..54882
                     /locus_tag="IV257_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813748.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(55423..55911)
                     /locus_tag="IV257_000598"
     CDS             complement(55423..55911)
                     /locus_tag="IV257_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8813749.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(56112..56378)
                     /locus_tag="IV257_000599"
     CDS             complement(56112..56378)
                     /locus_tag="IV257_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8813750.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(56378..56641)
                     /locus_tag="IV257_000600"
     CDS             complement(56378..56641)
                     /locus_tag="IV257_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8813751.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(56634..56906)
                     /locus_tag="IV257_000601"
     CDS             complement(56634..56906)
                     /locus_tag="IV257_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813752.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            57117..57302
                     /locus_tag="IV257_000602"
     CDS             57117..57302
                     /locus_tag="IV257_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8813753.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(57444..59267)
                     /locus_tag="IV257_000603"
     CDS             complement(57444..59267)
                     /locus_tag="IV257_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8813754.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(59865..62570)
                     /locus_tag="IV257_000604"
     CDS             complement(59865..62570)
                     /locus_tag="IV257_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8813755.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(62876..63823)
                     /locus_tag="IV257_000605"
     CDS             complement(62876..63823)
                     /locus_tag="IV257_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8813756.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            64013..64282
                     /gene="rpsN"
                     /locus_tag="IV257_000606"
     CDS             64013..64282
                     /gene="rpsN"
                     /locus_tag="IV257_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8813757.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(64458..64820)
                     /locus_tag="IV257_000607"
     CDS             complement(64458..64820)
                     /locus_tag="IV257_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8813758.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(64831..65952)
                     /locus_tag="IV257_000608"
     CDS             complement(64831..65952)
                     /locus_tag="IV257_000608"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8813759.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(65968..66318)
                     /locus_tag="IV257_000609"
     CDS             complement(65968..66318)
                     /locus_tag="IV257_000609"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8813760.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(66448..67959)
                     /locus_tag="IV257_000610"
     CDS             complement(66448..67959)
                     /locus_tag="IV257_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8813761.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(68281..69633)
                     /locus_tag="IV257_000611"
     CDS             complement(68281..69633)
                     /locus_tag="IV257_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8813762.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(69786..70862)
                     /locus_tag="IV257_000612"
     CDS             complement(69786..70862)
                     /locus_tag="IV257_000612"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8813763.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            71058..72803
                     /locus_tag="IV257_000613"
     CDS             71058..72803
                     /locus_tag="IV257_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8813764.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(73083..73520)
                     /locus_tag="IV257_000614"
     CDS             complement(73083..73520)
                     /locus_tag="IV257_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8813765.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(73504..74877)
                     /locus_tag="IV257_000615"
     CDS             complement(73504..74877)
                     /locus_tag="IV257_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813766.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(75199..75687)
                     /locus_tag="IV257_000616"
     CDS             complement(75199..75687)
                     /locus_tag="IV257_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8813767.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(75709..76311)
                     /locus_tag="IV257_000617"
     CDS             complement(75709..76311)
                     /locus_tag="IV257_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8813768.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        21910 a        16300 c        13947 g        24198 t
ORIGIN      
        1 tggagttaaa aaaacaaaaa tttttttata gttagaataa ttgcgttcta tatatatttg
       61 gtatttatta agttgccctc cagactccga agacagaatt ttattttcga tgcacaccac
      121 tattttattt ttttccgata ccaataaaat atcaatattc ttattttctc tgtaaacaat
      181 taaatcttca tagctattaa gtaaagcatc aaaataattg atagcagctt tggtatcatc
      241 attagaatct atataccttt tcaaaaacat tttaaaaaac tcatcagata aattgtgggt
      301 ttcatttgga gcaaacaaaa aagctaaaaa attactatgt cttatttcat ttttagatag
      361 cttaagtatc tgaaatatat ttgttttccc agtccattta gatagttttc ttcaatatca
      421 tccatatcct ctaatagatt ttttaaacac tttttttcat ctatcatgtt ttttctcctt
      481 gccattattc taactaaaat tttacgtatt tgtttctatg tctacattat ctttttaaca
      541 gtatctattt ttcttttgca acttacttgg ctaccaaagc aaataaaaaa gttatttata
      601 aatttctccc taccactacc tattgctatt atttaaaata gggataattt tttctcttaa
      661 aaaatatcat tatttgtggt atggagcccc cgcattgatc cggaaggcac ggtagatttg
      721 ctcgacaagg atcagacgca tcaattgatg tggatacgtc atttttccaa aagagatctg
      781 agcattgctt cttttcatga cggcttcgct gagtcctaaa gagccgccga taacaaaaac
      841 aaggtggctt tttccttgga tacctaattg gtcgatttgt ttagcaaaag attcacttgt
      901 aggatttttc ccttcaattg ctaaggcata gacgtattct tgttctttga ttttcgctaa
      961 gatccgttcc ccttcttttt cttttacttg tctcatttgc gcttcgctta aattctccgg
     1021 ggctttttcg tcaggaactt cgatcaattc gatttttgca taggcattca gccgtttgac
     1081 atactcattg ataccttgga tcaggtattt ttcttttaat tttcctactg agataatttt
     1141 gatattcatg ttttctctcc tttggtcttc atgctcactt tgtttattat aatcaatttt
     1201 ttcgtctata tgtttatttt ggctattttt gttaaaaaga tttccccaac agttatgcac
     1261 aacatccaca ggtttatcca cagaaaacag tgttatttta ccttatttca tcacattcat
     1321 taggcaaaca tacgttttat aagaatttat ccactttata cacaggctgt ggatatgttt
     1381 tatcccttat ttttccaatc ctatccgcgt agttttacac agtatctgtg gaaaaagaaa
     1441 atcaaatatt ttcatctgaa aacaatgatt tttcctaaag tttttcataa aaagttcaca
     1501 gtctcaaagt agactaaagt caacaaataa gactatacta acagtagata aatgaatgta
     1561 attggaggag gaacacatgg atagaaaaaa tgtgacacca aagatgaaaa aaagtaaaaa
     1621 taatagcctc tggcgtaagt taggtctggg gcttgttgga gggatcgttg gaggactact
     1681 gacagctggc attttttatg cagcgatggg cactggcaac aatagttcta ctgctacaag
     1741 cggcaatcag aattctgccg gtgaaacagt tgtggaaaat gtcaaagtca atgtagattc
     1801 tgatatcacc aaagcggtag ataaagttca agacgctgtc gtttctgtta tcaacttaca
     1861 aagtcaaaac caatcaagcg gttttggcgg actattcggg cagcaagaag aaagtagcag
     1921 ctcagatgat agtaatttgg aagcttatag tgaaggaagc ggcgtgatct acaaaaaaga
     1981 cgggaatact gcttatgtag tcacgaataa tcacgtagta gatggccagc aaggtcttga
     2041 agtcttgatg aaagacggta cgaaggtaaa agctgaatta gttggaacag atgcttattc
     2101 agatttagcg gtgttgaaga tcaattcaga caaagtcgaa acagttgcct cttttggtga
     2161 ctcaagtgct ctgaaggttg gtgagcctgc gattgcgatt ggttccccac taggatctga
     2221 atatgcaaat tctgtcactt caggaatcat ttcttccttg aaccgacaag tgacaagtac
     2281 gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa acagatgcag caatcaaccc
     2341 tggaaattct ggtggtcctt tagttaatat cgaaggacaa gttatcggta tcaactcaag
     2401 taagatcgca agtacttctg aatctacttc taatgtaagc gtagaaggaa tgggatttgc
     2461 tattccaagt aacgatgtag tgaatatcat caatcaactg gaaaaagacg gaaaagtgac
     2521 acgtccagct ctaggtatca caatggtcga cttatctgcc gtttctactc agcaacaaga
     2581 acaaatcttg aagattcctt catctgtaac taacggtgtc atcgtgacaa atgtccaaac
     2641 agctacaccg gctgaaaaag ctggattgaa acaatacgac gtcattacta agatcgatga
     2701 caaagaagtt tcttctggtg tagaacttca atctgctttg tataagaaaa aagtgggaga
     2761 ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa acaactactg ttgaattgac
     2821 gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa aattaataaa aaagaggttg
     2881 tgacaaagac ttgtcacaac ctctttattt tggaaatgct tctccgacta cggcatcctc
     2941 aggcaatact aagattcctt tttttgctgg agcatttggc aaatgcaatt cacgagcgga
     3001 acagatcatt ccatagctat ctacccctcg caaagttcct ggccagatca tcattccatc
     3061 tggcatcata gctcctggtt ttgcaacgac aactttttgt ccagcttcga tattgggtgc
     3121 accgcaaacg atttgcaata ctgtaccatt gtctacttct gtctcagtaa tcgataaatg
     3181 gtcagaatca ggatgtggga cgcatgtttt tacgtacccg acaacgatct tcggttcatg
     3241 atcagcttca agctgttctt cgaatcctgc ttctttgatc aatcgattca atttcgctaa
     3301 ttcctcatct gttaagctta cttggccgtt cccttcgatt gttagatgat cggagatatg
     3361 gaaaaagttc caagcaacaa cttgaccttg ttcatctttg atccgagcaa cattccattt
     3421 gcgttcaaca gtgttttctg ttcctttatc gtcggctata ataaccatca aggtgtctcc
     3481 gacgtgtgct ttgttataag caaaaatcat gttctttcct ccattatcct ttgctgagat
     3541 ttatcttaac aaaaaattgc attcactgca tgaaattttt ttcatgcttt atttccgaag
     3601 aagcctcttc tggaacacca tttattcgta acaagggact gtgacaagac ttttgtcaca
     3661 gtccctttac tgttttagtt aattgattaa aaaggtcgta caagttccac ttgcttaatt
     3721 acttggctgt tcttcttcgt cagaaacgat caagacatca caaggtgaat gacgtatcac
     3781 gtagctggct acacttcctg tcataaagcg ttcgactgca tttagacctg actgcccaac
     3841 catgattaga tcgatctcat atttttttgg caatttgact gccatggatt cacgaacaga
     3901 accatatgca ataattcctt ctatatttgt gtaatcaaca gatttcccat attctttaca
     3961 ttcttcgatc atttccttgg ctgtttcagt ttgctgatcg actagatttt gatcaatagg
     4021 tgcatagccc ataaagttat aaaattgctg atcgattacg tttgctacat atactttccc
     4081 attattacgg cgagccactt ctaccgcttt tttgaaagca gcaagtgctt gttcactacc
     4141 gtcgatacct actaaaatat tcttatattc ttgattcatc ttgacccctc ctaacagatg
     4201 tcagcctttt gttacacttt ctaggaacga ttcgatttcc tgttttgttt tacgatcttt
     4261 attcactaga cgacctaatt cttttccgtc ttggatcacg acaaaacttg ggataccaaa
     4321 aatgttccat tcagcggcta catctatgta cttgtcacgg tctacttcaa taaattgcca
     4381 atcagaaaag tcattttcaa tttcaggcat ttgcggtttg ataaaacggc agtctccgca
     4441 ccagccggca gtaaagaaaa atacactctt tccagtttct acgtagcctg ctaattcttc
     4501 taaactattt gggataatca ttctttgtcc tctactttct gtttatgtat ttttcaatta
     4561 aattatacac ctttctttcg aactaacaaa cattgatcct ctttttataa aaaaaatctg
     4621 gcaaaaagca atcttttgcc agattttata gtttcactat cgcattattt tccgtatacg
     4681 atcgtattta ctgttgattt atccaaacct ttcagtgttt gaatcaacat ttctcgtgca
     4741 gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg tcccactacg
     4801 ccaatcactg tacttgggat tccttcgttt tgtgtatgag cagcacctgc atctgtaccg
     4861 ccttttgaaa caaagtattg ataagggata ttgtttgttt cagcgatatc cagcaagtac
     4921 tcgcgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa tgtaccttct
     4981 cctaaatgtc cgaatgtgcc atttttcgtc actgtatcat ctgctgcaga gcagtctact
     5041 gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg caagcctact
     5101 tcttcctgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt ttgcaacgct
     5161 tctaaagctt ctaataccat tgtacagcca tagcggttgt cccatgattt actgataata
     5221 ttctttccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc tcctggaagg
     5281 acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa aatatcggtt
     5341 acttctactg atttttgtcc gcttgttcct cgcaataaat gtgggggaac ggaagaagaa
     5401 atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgctgagc agaaacaacg
     5461 taaggattcc agccgcctag aggtacgact ttaaaaagcc cgttttcttg gatttgtgtc
     5521 agcatgaatc cgacttcatc catatgtgct gcgaccatta cacggggtgc atccgcttct
     5581 tcatgatggc gcagaccaaa aatgccgccc aaaccatcta actgtaactc atctaccagc
     5641 ggttccatat gactttccat ataagcgcga acatcttgtt caaaaccgct tgttccttgt
     5701 aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta ttcgtctaaa
     5761 tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg attttttctg
     5821 cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta tttcaaagaa
     5881 aatatgagag tgtggtaaaa taagcacaag gagtaacaag tgagaagaag gaggtatgtc
     5941 cacatgtatg aagaaagcga actaagctat tttaaaggcg ggttagcagt tggaatcggt
     6001 ctaggattat tgagcggggt agcttcaacg atttggtatc atcgcaaaaa agtattgtca
     6061 gcagaccaag tacttgaaaa tatcaagtca tcttttctaa aagaaggtgc aatcgaaggt
     6121 tcttggatct cttttgaaaa ggaacctatt aggaaattcg ccgttcggtc gagtgcttat
     6181 cggggaggtc tttctcgaat agaagatggc gaaactgttt attatgaatt tttagcagat
     6241 gctcatacag gaaccgtatt agaaatttca agaaaaaaag gtgcaacaga ttaatacgtc
     6301 aaaaaagtga gagaacgaat acgcacccac tattcaaaag agaggtatca tgtccttctt
     6361 ttgtagtggg tggtttttta gatagagctt tccaaacata tattccagta ttttcttttt
     6421 aaataaatga tatttagcgg gaaaaactag catttccttt gattatgagg tatttttaat
     6481 ttagcttttg ttttctatag ttaaggagag tgaaaaatgg aacaacaaga aaaaattgct
     6541 gttctgattc cttgttacaa tgaggaagca acaatttcaa cagtcattgc agactttaaa
     6601 cgagaattac cagaagcaga tatttatgtc tatgataata attcgaccga tcgaacatac
     6661 gaattggccg ttcaaggtgg agcaatcgta aaaaaagaac ctcgtcaagg aaaaggaaat
     6721 gtgatccgcc aaatgttctt tgatatcgat gccgactatt atttaatggt agatggcgat
     6781 gatacttatc ctgcagaagc tgtacatggt ttgctcgaga aattacgttc tggtgaagca
     6841 gatatggtca ttggtgatcg cctatcaaat ggaacctact ttgatgagaa taagagacct
     6901 ttccacgatt ttggaaataa ccttgttaga aatacaatta ctagaatgta taaaacaaaa
     6961 attcttgatg tcatgaccgg ctatcgagga ttcaatcgta tctttgtcaa aagttttcct
     7021 attatgagtt ctggttttca aatcgaaacg gaattgacga tccatgcttt agataaaaaa
     7081 ttcaagttcg ttgaaatccc aatcgattat cgtgatcgtc ctgaaggcag cgaatcaaaa
     7141 ttgaatacat tctctgacgg attcaaagtg atcatgatga ttattaaaat gtttaaagat
     7201 tataagcctt tgcttttctt tgggatttgg accattttct tctttttatt tggattggtc
     7261 acggggattc ctgtgattcg cgaatatatg ctgacaagct ttattacacg catcccttct
     7321 gcgattttaa gtactggatt gatgacttta gcacttcttt cattagtaac agggttgatt
     7381 ttggatacag tcgtcacgaa cgctaagaaa gaatatgaat tgaatctata tcatgtttat
     7441 catgaatacc acagtagaaa gtaaaaaaag gatcaagact gcaaaaataa tttgcaattc
     7501 ttgatcctct ttttaattaa ttatttcgcc aaacgataga tggcatctgc atagattgct
     7561 gttgctctga acaagtcatc aagactcata aattcgtttg cttgatgcat tgtatcagta
     7621 tatcctggga acatcgctcc ataagcaacg ccacgtttca acaaacgtcc gtaagttccg
     7681 ccaccgatga tttgttcgta gcctttttct ccagtgtggt cttcatagac ttgcaataat
     7741 gtagccacca atggatcatc cattggtacg taatgtggtt ccattacgcg agcacctcga
     7801 gtaactgttg cgccttcttg ccccactgtt tgttcgatgt cactttgcaa tccgtctact
     7861 gttacacctt ttggaaaacg gaaattcata ttgatgaagt tcgcttcctc tgtaccattt
     7921 tcttcaaaag caaatagtcc agcattcatt gtcaatttgc ccattttttc atcttcataa
     7981 gcgactccta gtttttctcc ataaaaatct tcatggacat aagcagctgc tacatggata
     8041 aattgtttag cagatcctag gaattcatag ttatctaaga acaatgccaa gaaagaacct
     8101 gcattgatac cagattgtgg gcttgcacca tgtgcgcctt taccaactaa ttcgattttt
     8161 acatacgtat tgtccgcttc aatcgttccg cttactggat ttgcttcgat gaattggtaa
     8221 aatgcttctt ccatcgcgat agctgcatct gcacttggta cttgaagtgc tgcagttgct
     8281 gttcctggca ccatattttc gcgaagtccg gaaacaaaag atttcaagac ataatctcct
     8341 gcattgtcgc ctttgaagcg taaagctaaa gaaacatttc ctttttcacc attgatgatt
     8401 gggaattcag catctggtga gaaaccaaaa tctggttctt cttcatgttc gaaataataa
     8461 gccatatcgc cccagccgct ttcttcatcg ctgccgacta cgaagcgaac tttttttgat
     8521 agtttcaagt caagttcttt aatgatcttc atcgcatagt aagctgccat acttggacct
     8581 ttgtcatcgc ttgatccgcg tgcaaagatc tttccatcct tgattactgg ttcataagga
     8641 tctgtgtccc agccatctcc tgcaggaacg acatccatgt gtccaaatat ccctagcgtt
     8701 tcgtctcctt cgccaagatc aatatgccct gcatagttat cgacattttt tactttaaat
     8761 ccatcacgtt cgccataagc taacatatgt ttcaatgcgt cgcgaggtcc aggtccaaat
     8821 ggtgcatctg gtgttacttt tgagtcatcg cgctcgctgt tgacacgtaa aagatttttc
     8881 aagtcttcaa gcaagtcttc tttacgtgct tctacttctt tttgccaatc aattgtcata
     8941 aagttaatcc tccacttatt taagtcttcg tgttcatgat accacaaatt acagaaaaaa
     9001 atagttcaaa aaatctgaca attccttttc ttatctgttt ttctctaaaa aaaatatacg
     9061 ctataatgag taaaaggagg tttttagatg ctgataaacg aacaactagt gaaaagagtc
     9121 atcacacctc gtccaaaaat ttcacataaa ggaacatttg gacgtatcgt tttgattggc
     9181 ggcaatgaac aatatggcga tcattatgag tgctttagct gccgtacaca gcggagcggg
     9241 tctcacgact gtagtaactg atgaaaaaaa tcggagtgct cttcacagtc atctgccaga
     9301 agcaatgatt gtagtttgga cggatactga ccagtttctt tccgtaataa agcaagcaga
     9361 tgtactcttg attggtcccg gattgggtac ttcttctaag agtttggaac gtttcaaaca
     9421 agtactagaa aaccaatcaa aagaacaatg gctggtgatt gatggctctg ctttaagttt
     9481 acttgctgct aatcatcttt tgctacccta tcccgaacaa actgtactta cacctcatca
     9541 aatggagtgg caacggttga gttctctacc gatacaagaa caacgggata aagaaaacaa
     9601 aaagaagcaa caggaaatcg gtgctgtgct cgttttgaaa agtcatgaga caaaaattta
     9661 tggtcaccag cacttctaca ccaatccttt aggttctgct gctatggcaa cgggcggcat
     9721 gggtgacact ttagctggga tgatcacagg atttttagcc cagtttcctc aaaatgacca
     9781 aacgattgca gcagctattt atcttcatag tttcattgga gaccagctgg caaaagaaca
     9841 atatattgtt ttgccgacac aaatcagcga agcacttccg aaatggatga attatttttg
     9901 taaaaaatca atggaaaacg aggcatgagt catggagaaa tttacaggtt atgtgcgaga
     9961 acctttttat tatgaaacag atcaaatggg gattattcat cacagcaatt atatccgctg
    10021 gtttgaagag gctcgggtag ccttattggt acatctaggg tttccctata aaaaaatcga
    10081 agaatctggg atcatcatcc ccgttctgga agtaaattgc agctataaag aaatgatcca
    10141 ttacggagaa aaagtgagca tccatccaac tatccaaaag tatacgggga cacggttgga
    10201 ttttagctac gaaattgtgg gaactgaaga caaaaaatta aaaactactg gcagtagtaa
    10261 acactgtttt ttactttctg attcgcaacg attagtgaag ctgagcaaag tcaatccgga
    10321 gttagatcag ttattccaag cgtacgcttc tttggatgat gtaaattctt aataaagacc
    10381 gtctaagcat ttttcttgta ttgtgtcgag tagttcgtat aatgaaaaga aaaaagcagg
    10441 tgatccccat gtatgtcgtt aaagtaatgc acggttacat cgataaaact ggctgtcgca
    10501 ctcgggaaaa aaacttagac aatctgctca ttttcaaaga caaaaaagaa tccgaagctt
    10561 ttgctaaaag aatcggcggt cgtgtcaaac cgatccaaga ggtacgacca gattgattca
    10621 atatttttct tttcgaggct ctaataggca tgcctatcag ggcttttttt gcagactgtg
    10681 tttttatgcc cacacgattt gtaagcgatt tatgttataa tagattatat ctgcatagaa
    10741 aggaaggtta aatggttatc aataatttgc aacagcaagc ttatgaatct atacgtaaac
    10801 aaatcattta ttccgagtta gcacctggaa gtaaaatctc cgataaagtg ttagaagaac
    10861 aactagctat cgggcgtaca ccgattcgag aagcactgat ccaactgcgc aatcaagagc
    10921 ttgtaaaaac cattccacag tccggcactt atgtttcaaa aatcgatatc cgatctgctt
    10981 cattagcccg ttatacgcgg gaaaagttgg aaaaaccgat cctgcaagaa tgcagtgcga
    11041 aaatgaccaa gcaagatcaa gctaaactag aaaaaatact agaacaaacc gatcaggcag
    11101 taatcgctca aaataaaaaa gctttttttg atttggatac tgattttcac cgaacttgtt
    11161 atgaaattgc tggaaaacga gaaatttggg attggctaga aagttatagt acgcacttga
    11221 accgtttcag atggttacgt ttgacgattt cagaattaga ctggggtcga gtattagatg
    11281 aacatcagac aatgcttcaa tcaatgattg atcataactt tgatgaagtc ggatttcttt
    11341 gcacgatgca tttacatatg attatcgagg agcaagaata cgtgattcat aattaccccg
    11401 attattttga agatattcag gacagaccca tcaaataaga ggttttggaa tcagcgtttt
    11461 gacctgagta ttagagaaag aaacggcaaa atagttcctc atattttgcc gttttttttg
    11521 agctaatacc gagggtcagc tgattgcaca ccgtttattg agaggttgtg aaaaagctgc
    11581 cctgcatcaa gtaataagaa cagccaagta aaaatagctt cttatgtttt taacttggct
    11641 ggacttatta ccgcagatgc aagcttttga acaccgtttt ccaagaggtc gtgagcctgc
    11701 cgttcatctc cgagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgttgaata
    11761 ttttgacttg tgaccgagga gttgacagga gaacatcgtt tattcggaaa aaaaggactg
    11821 tgacacgact tttgtcacag tctctttatt tgtaatctaa atcatactcc gtacatcttt
    11881 tttctatttt ttttatttct tctaagatcc cttggctacg aatttcatta tatggccaaa
    11941 gtaagactgc ctctaataca taaggcgaaa aatcgctttg attcatctct tttgaaaaac
    12001 tcatcgctgc tatctcgctc aatgcacgaa tgtttgattg atgtgtataa aaaggcaacc
    12061 gccacagcac aattttttcg ctttgcgtat aaattccctt aacagaagct tcgatgtggt
    12121 gaaatagttc atgtccaata atcaaagctt gcgctgtttc tttagagatc gaagctagtc
    12181 ctgaacattt taatttttct aacggctccg tcatcaatcg aatcccttca tcttctgcat
    12241 aagttgcaaa agtgattcgt tcgtttattt gctctgattc acaggaaacg attggaacag
    12301 ccatttgttc acaaatttct cctacatcgg caaaagggta cttttttttg atgctacatg
    12361 ccgtttcgat accacaatcg attgattgtt gtataagaat tttctgcttg gacaaaggaa
    12421 tctttttcca aaataaatct cgccgaaact ggtagaatcc ccaaatttct tcggggatct
    12481 gttgtaattc gtttagtaac tcttctaact tatgtttgtt tgatatttcc ggcaacgacg
    12541 atcacttctt cctccggttc catagtagat acttccagat ccatcaaaag ctgtagttgt
    12601 tccagatccg tcgctgtaaa atcagagact cttgctccta agcagagata aaattctggt
    12661 cgggcaatca attcagtttg ataaactgcc atatcttccc aaagttgttc gacagctgtc
    12721 atataattgg ctgcagttgt tttcatacat gaagcatgtc cacgttggac cttgataaat
    12781 cctttttgat cgatgatccg aagacttcca gcattttttg aacgattctg ttcgccgcat
    12841 acataaaaga aaggtgtatt ttccaataag caaacttcat ttttatttaa tcgcatatcc
    12901 tctgcagcaa gctctagtgc ttcttctgtt gtgatctcct tggttagatc ggttgctttc
    12961 acttccgtag aaccagtagc gatagccgtt acttttgaag tctgtggatc gatttctacg
    13021 tggacttcga tcgattctgg tgtagcgcct gattcaatcg ctttattcat cgcttcattt
    13081 ttcaacgatc gtatatcttc tttacttggc gaagggatga tacgttctac aacatctcgg
    13141 accattgcaa gtgctacacc aatagaagaa atcacttcgg cgttttcagg aatcgaatat
    13201 ttcacaccca ttttattact aaagtaagta atcaatgagg cagcgccgcc ccctacacct
    13261 acaagagaaa tctgatcttt ctccaagtga tacttgtctg ccaattctaa aatcaccggt
    13321 tcaatcttag cataggattt ttccatgatc tgttctgcga tgtcttcaac cgtcgtatga
    13381 caataatcag ctaatgcttg gatggctttt cgtgcagatg ggacatttcc ataagaaaaa
    13441 tgttcttctt gcactaatcc aaggacattg gctgcacatg tatttgtaat agtcacttct
    13501 tctccgtctt ccatcactac cttgacgtaa tcagcagggt ctccttcttt aggagagaaa
    13561 aattcaacct taggaccttt gattttttct gtctccgtaa atactgcata atctagaccg
    13621 gcaatatgtg cagaacgtgg accgacatcg atgattcctg actgattagc ccgtaccatc
    13681 gaaccgcctg ccacacctag aacccgaaca tctaatgaag agatatatgt tgggtggcca
    13741 cctacaattg aataatcgat tgctggacgt ccatttttaa tgaccccgat attggttgtt
    13801 gttccgccta cttcaaaata aacaccatta gacgctctca agtacatcaa agagcccatg
    13861 actgatgctg ctggaccgga aagcatggtt aaaacaggtc gttttttcat ttcattgatt
    13921 tccattactc cgccgtctcc gcgcataatc atcaaagaaa cattgacccc tgcttctcgt
    13981 acggaatctt ctgtagaggt tgccgtatct agcatttttg gtaaaataga tgcattgatt
    14041 gctgctgtcc gagttcttct tgtcaatcca tataattttg tgatgtcaga agccatcgtt
    14101 gtagggatcg attttaccga tgctgcttca taaacaactc gttctggttc cccattatct
    14161 actccaaatg ccatagaaga aaccaagacc tccgctcttt ctctttctaa agaagaaatc
    14221 gtttctgaaa cacggtctac atttaagtgt ttgacaggta agaatgcatt gacaatctct
    14281 atttttttct tattccctaa atcgatatcg ttcaatctag tttgtcgttt tgctaaaaaa
    14341 ccttctaatc caccttttgc catcccgatt acgccgactt ttgctacgtc tccttcaatc
    14401 agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca caacatcttc tggactgata
    14461 ttattttcca gcaaacaatt ttgaaaagac tgaacgacac ctgcggctac tccacggaca
    14521 tcatcgtggg ttgttttcac tgaagatttt ccgatgattt cgtgcgtcgc attatcgatt
    14581 gcgactgctt tcgtatgtgt accgccaaca tcaattccca ttcgcacaaa acgtttcatt
    14641 ttcttttcct ccattcatct aatcgttttt atcgacgttt tgtttactag gcgttcccgt
    14701 acaataagaa cgtgatcagg cataagatcg ctccgatgat ccaaccagtt gggatggatt
    14761 tcttcatata ttctcgagaa gttacttttg tataacctaa tccccaagca acccaagatt
    14821 gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc aaataacggg aacaaaaagg
    14881 ctggcgacat cgttggatta acagctaata cgacagctaa aatagctgag cctgaaccga
    14941 ctagattcat tggaccgcgg aaaaatccta gtggtgtcaa tatagcaaat acgattgcca
    15001 agactaatgg tgattgcggt atccaatctc caaaaatagc tgaaaaataa ggagaagcat
    15061 acgcagctga attattgaac atcgctaagg tcatcaagaa accaaccatt ggcgcaacat
    15121 ctactgcgcc atctgtaaat aatttcgcaa actgacgaca gatttcttta taacctccgc
    15181 gcaattttcc agtaaacagc aatgcccaga tacctgctag aataaaacct aagatgattg
    15241 gaagatctaa aagaacgacc cctaaaactg gtaataatac ggctaaccat gagatcattg
    15301 gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc atagcgtttc tttttgttca
    15361 ttgatatatt agcgaccgtt agtaccacaa ctaagcttac aatcatccca atcataccaa
    15421 tttggaaata gtcattataa gtatagcttt cagccgctgg attgaaacca gcataaatcg
    15481 tttgatattg tttgaaattg acgatattag caaagattcc agccataatc gagcccataa
    15541 atgagaacag tgcaaccggt actgaaatac ctaatgacat caagattggc aacacaataa
    15601 ctgcaataga aatcactggt ccgattcctg tcatcgacat gaatataaca gcggttacaa
    15661 tacatagtaa gctcatcgtg attcttggct tgtcccctcc aagttcaact acctttcgaa
    15721 tcagggtagc tgcgattccg ctatctacta gaacacgccc gaaaaaggca ccaaagaaga
    15781 catttaccaa gatcgatttg gcgtactcag ctggccccgt cgtatagata tgcgtcaaca
    15841 catcaattac tccttgccct tccatcgccg ggtttggtga aaacgtgttt ccgactagcg
    15901 ctaatcccat ccatacagta gccatgatgg caaagccgac catcaagttg tagcctttta
    15961 cacagtacca gaccatgaaa aagaatgaga taactaacaa aataccaata ataatatcac
    16021 tcatgttatt cctccgtcca ttttgttttt ctgataagca aactaatata tcagtcaaac
    16081 tcattctaac ttatgtagtt agcgctttca ttaaagggtg accataaatg ttcagttttc
    16141 aattaaaaaa ccttaattat gctattttag acttatacgt tattgtcctg tttcatttga
    16201 tggaggattg taaataaaaa aagaggaaag aacataactt ttcgttatct tctttcctct
    16261 ttataattgt ttagcttatt tagccaaagc ttcttttgct tggtcagcca atgcagtaaa
    16321 tgctgctgca tcgttaacag ccaagtcagc taacatttta cggttgatgt cgatttcagc
    16381 aactttcaaa ccgtgcatta attttgagta gctcaagcca ttcatacgag ctgctgcgtt
    16441 gatacgagca atccacaatt tacggaagtc gcgtttcttt tgacgacgat cgcgatatgc
    16501 gtagttgtat gaattcatta cttgttcttt tgctgtttta aataaagtgt gttttgaacc
    16561 gtagtaacct tttgctaatt ttagcactct tttacgacgt ttacgagtta ctgttccacc
    16621 tttaacacgt gccatgtata attcctccta ataaaaataa atcttcagat tctgttattt
    16681 gtacttctta tttcattttt gctagttgtt ggcgaatacg tttgaagtcg ccactagaaa
    16741 ccatgcttgc tttacgcaat tgacggcgtt gtttttttgt tttgccgtgg aaacggtgac
    16801 ttgtaaacgc gcggaagcgt ttcaatccgc ctttaccagt acgtttgaaa cgttttgctg
    16861 atccgcggtg tgttttttgt tttggcatga ctatatttcc tcctcaaaat ctttcgttat
    16921 ccgactatcg gctgtccaac agccagttag tcattcaaat tacttactgt cgtttttcgg
    16981 tgccagcgtc aagaacatgc tgcgtccgtc cattttcgct ttttgttcca ctgttgcaat
    17041 atccgcagtt tcttcagcta agcgattcaa gactttttga ccaatttctt tatgggtaat
    17101 ggcacggcct ttgaaacgga tagaagcttt cactttatcg cccttttcca agaacttacg
    17161 tgcattacga agttttgtat tgaagtcatt caagtcgatc gtaggactta agcgaacttc
    17221 ttttacattg atcacttttt gttttttacg cgcttcgcgg tctttctttt gttgttcgaa
    17281 acggtattta ccgtagtcca tgattcgcgc aactggtggt ttcgcaccag gggcaactaa
    17341 cacaagatcc aagtttgctt gttctgcaat attcaatgct tctgcttttg tcttaactcc
    17401 taattgctca ccatctgaac cgatcaatcg taactctcgt gcacgaatgc cgtcgttaac
    17461 catcatatcc tttgctatgg tcattcacct ccaaatatta tgagagaaag aaaagcttac
    17521 cgaataaacg gcaataaaaa aacgagttct cttacagaac ccgcgcgtac ttcacctaat
    17581 agaaccagtg aaaactatct agcccagtga cattatcaac taaggcgaga agcgggtgct
    17641 tctgctttta tttctcaacc atcatagtat agcaaaaact aatctttttg tcaatgtttt
    17701 tcatacagtt tgtcttcctt ttctgaaaat gattagatta gcagactttc tagcagtgat
    17761 tcccgcttct ttgctatact aaagaaaaac gaggtgaacc cacatgaaaa aaatcgaacc
    17821 tattccacct tctcttcctc ttcttacaaa gggagagttt tatttagaag atgaggagat
    17881 cttctctggt cttctgatca acgggggtga ccaaagctat ctcgatgcca aaaatatggt
    17941 attaagagag agtcatatcc aaaaacttac gatgcaaaaa acagttttac gtcattttga
    18001 atgcagtaat gtcatttttg aaaaatgtga tttttccaat ctggagtgga ttggtggcag
    18061 tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt acgaattttg ccgaaagtta
    18121 tcttcgagac tgtactttca ctgattgtat tgcttcgatg gcttccttta gtagtacaaa
    18181 cttgaaagct gtctccttcg atcattgtca attagaagac agcgaatttt atgaggtcac
    18241 ttggaaaaac ctttttttgt ctgacaatca gttgaccggt tcgaactggt ttcgaacttc
    18301 tctgaaggga ttagacttta cgactaattt cttcacagca atcgctttat ctcaagattc
    18361 tttggcggga ctaatcgtca accaagagca agcacttgtg atcgcccaag cccttggttt
    18421 gcagatcaaa gactagatca acgaatccgc cgccacagtg caataagcat ccaaatagat
    18481 aagaccaaac tgatgatcaa ggcgattccc catccgaaaa cggaatgggt aaagggtaac
    18541 tggagattca ttccgaagaa tcctgtcaca atagttggaa cggtcaagat cagcgaccag
    18601 accgtcaaaa atttcatcgt gtcattcaag ttgttattca gcaaattatt ataagtgcca
    18661 gagagctggt ctaaaatctg cgaagctaag ttcgtcattt caactgcttg tcgtgcttcg
    18721 atcagcgcat cgtccaactc ttctttttcc ttttctgaca acttgcgata aatagccaat
    18781 gccttgattt gttctaaaag tactacattt tgttttgttc ctgtaactaa gtaaaccaaa
    18841 ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg ttgttttttc tcttaatttc
    18901 agattaagcc gctgacgttc actattcact tcctcgatta aaggaaaaaa ggaatcagaa
    18961 atcaaaaaca atgtacgaaa caataaactg tatacactca tgtctggcat tcgttccaaa
    19021 agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg tggtaaatgt aaacaaaccg
    19081 tcttctttta aaataaaagc gataggacta gtttcataat gattatcgac tttttgctgg
    19141 tgcggaacat ttgatacaag cagtaacgct tcatcaaaag aatcgtactc tacgcgcgcc
    19201 cgttcatttt tatctagaga ataagtcagc atctcgtcac taatgccata ttcttctttt
    19261 aagtttttaa cagctagcac atcgttttgc tgtacattga tccaaaaatt acggtcttga
    19321 ttaaaatagt gtttttcccg catactctat cctcctgaca tttgtttcca gtataaagaa
    19381 aaaagtcgct caaataaagg tttacccttt atttgagcaa ctgtaaatca aatggctact
    19441 ttggtttggc gaatctctgc taatttttct tcgatcattt tcaagacttc ttcttttgct
    19501 tgaggatctt ccacaaaatt atagcggtct ccatcgattt ggatttttgg actttctttg
    19561 tattcttcat accattgatc gtaacgtctg ttcaattctt tgtaataatc gtatagactt
    19621 ggatcaaagg acaattgttc atacgaacga ccacgttttt cgatacgctc caacatcgta
    19681 tcaaaagata cacgaatatg aactaataaa tccggatgtt ttttatgggc agcataggga
    19741 agttcttcca tcatgttttc cagcagttca tcatacacac gaacttctgt ttctgttgct
    19801 cttcctagat ctgcatttag atggaaaagc agtgaatctt cataaatcga acggtccaat
    19861 acattattgt catctttcat tgcttgtttg atgcttgcaa aacgtttatt caaaaaataa
    19921 atttgcagca aaaaagcata ctgttcaggg tttgcataaa ataagggcaa gacttcatta
    19981 tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt cagccatcat ttctgttaaa
    20041 cttgattttc cggcaccaat tgtacctgct aaaacgatca cgctcattta cctccagctc
    20101 acttcatcac taaatattgt gtttaactcc aaccttttta aatatacccc caatatctag
    20161 tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa agtcaaaaaa ttcttaagaa
    20221 aaaagtgttt tttctttaga aaacgtattc ttagttttac ttctgagatg gttttagtaa
    20281 aataaaggag gaatgttttt tgagaccctt agtggacaaa aaaattcgtg aagcagcgca
    20341 actcgttttg ggcgctttat tttttgctta aaaatcactt ttacgtgaga aggagttaga
    20401 ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat catattgatg ggcgagcacg
    20461 cggttgttta tggagaacca gcgattgcct ttcctttcta tgcaacaaaa gtcaccgcat
    20521 tccttgaaga gctggatgca atggacgatc aactggtttc ttcctactat tcaggaaatt
    20581 tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt attcattcac ttaaaaaaac
    20641 agcatgacat ccaaaaaaac ttgcaactga ccattgaaag cacgattcct gctgaacgtg
    20701 gaatgggatc aagcgctgca gtcgccacag cagtcactcg tgctttttat gattacttag
    20761 catttccttt gtctcgtgaa atactattag aaaatgtcca gctttcggaa aaaatcgccc
    20821 atggtaatcc tagtggaatc gatgcagccg ctactagcag cttgcagccg atttatttta
    20881 caaaagggca tcctttcgac tacttttctt tgaacatcga tgcttttttg attgtcgctg
    20941 atacaggaat caaaggacaa acaagagaag ccgtcaaaga tgtcgctcac ctctttgaaa
    21001 cccagcctca tgaaactgga caaatgatcc aaaaattagg atacttgacg aagcaagcaa
    21061 aacaagcaat cattgaaaat tcaccagaaa cgttggcaca gacaatggat gaatcacaat
    21121 cacttctgga aaagctgaca atcagcaatg attttcttaa tctattgatc caaacagcaa
    21181 aagataccgg ggcattgggt gccaaattaa ctggcggtgg acgcggtggc tgtatgattg
    21241 cattagcaca aacaaaaaca aaagcccaag aaatcagtca agcacttgaa gatgcgggtg
    21301 ctgctgaaac ttggatacaa ggattaggag tacataccta tgtttaaagg caaagcacgc
    21361 gcatatacca acattgctct aatcaaatat tggggtaaga aaaatgaaga actcatcctt
    21421 ccaatgaaca acagcctttc gttaacattg gatgcatttt atacagagac agaagtcatc
    21481 ttttctgata gctatatggt agatgaattt tatctagatg gcaccttgca agacgaaaaa
    21541 gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta aagaagccgg cctctcacta
    21601 aaagcttcag taatcagtca gaatttcgtt cctaccgcag ctggattagc ctcctctgcc
    21661 agcgggctag ctgctttagc aggcgcttgc aatactgctc ttaagcttgg attagatgat
    21721 ctctctcttt caagatttgc tcgacgcggg tctggttcag cttgccgaag tattttcggt
    21781 ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt cttacgctaa gccagtccct
    21841 tccgattctt tcgaagacga tttagcaatg gttttcgttt tgatcaacga ccagaaaaaa
    21901 gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa catccagttt ttatcaaggc
    21961 tggttagatt ccgttgaagg ggatctatat caattgaaac aagcaatcaa aacaaaagat
    22021 ttccaacttc tcggagaaac gatggaaaga aacggactaa aaatgcatgg aacgacttta
    22081 gctgcccagc ctccattcac ttactggtct cctgacagct taaaagcgat ggatgcggtg
    22141 cgacaattga gaaagcaagg cattccatgc tactttacga tggatgctgg ccctaatttc
    22201 aaagttttag tggaaaacag ccatttatct gaggtacaag aaacatttac caaattattt
    22261 agcaaagaac aagtaattac tgcacatgct ggtccaggaa ttgcgattat tgaataaagg
    22321 gagattttta tgattgaagt atctgcacca ggcaaactct atattgccgg agaatatgca
    22381 gttgttgaaa caggccatcc agcagttatc gctgcagtcg atcaattcgt gacagtcact
    22441 gtagaatctg cacgaaaagt cggaagtatc caatctgcac aatatagtgg gatgcctgta
    22501 cgttggacaa gacgcaacgg agaattagtt ttagatattc gggaaaatcc ttttcattat
    22561 atccttgctg ctattcgctt gactgaaaag tatgcacaag aaaaaaacat tcttttatcc
    22621 ttttatgatc tgaaagtaac aagtgagtta gacagttcaa atggccggaa atatggattg
    22681 ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct tgaatgtttt ttacgcgttg
    22741 aatttatctc agttggagat tttcaagatt gcagcactag ccaatttagc agttcaagat
    22801 aatggttcct gcggcgacat cgctgctagc tgttatggtg gctggatcgc tttctcaacc
    22861 ttcgaccatc cttggctcca agaacaagaa actcagcatt ctatcagtga gttacttgcc
    22921 ctggattggc caggtctatc cattgagcca ttgattgctc ctgaagattt acgtttattg
    22981 attggttgga cgggtagccc tgcctctact tctgatttgg tcgatcaagt tcaccgttcg
    23041 agagaagata aaatggtggc ttatcagctt ttcttaaaaa atagtacaga atgtgtcaac
    23101 gaaatgatca aagggtttaa agaaaataat gtaacgttga tccaacagat gattcgaaaa
    23161 aaccgacaat tactgcatga tttatctgca atcactgggg tcgtcatcga aacgcctgct
    23221 ttgaacaaat tgtgtaattt agctgaacag tatgaaggag ccgcaaaatc ttctggtgca
    23281 ggtgggggcg attgcggaat cgtaattgtt gaccagaaat ctggcattct tcctttaatg
    23341 agtgcatggg aaaaagcaga aatcactcca ctgccgttac atgtctatag cgatcaaaga
    23401 aaggaaaacc gatgaatcga aaagacgaac atgtttcact agctaaagcc tttcatgata
    23461 aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa ccctttgccg caaatagcag
    23521 tttctgatgt cgatctaagt acacaggcag ttgggtttac actaagcagc ccattttata
    23581 tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat caaccaagat cttgcaatcg
    23641 tcgcaagaga agcagatttg atgattgcca ctggttcagt cagcgctgcc ttgaaagtcc
    23701 cttctttagc cgatacttat acgattatgc gacaagaata tcctcacggg aaaatcattg
    23761 ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga agctatccga ttgtttcacg
    23821 cagatgcctt acagattcat ttgaatgcac ctcaagagtt ggttatgcct gagggagacc
    23881 gtgattttac caattggaaa gtacttattc aagaaactca aacagccatt gacgtccccc
    23941 tcatcgtaaa agaagtcggt tttggcatga caagagaaac gctcaatgat ctagctgctt
    24001 taggagttca tacagttgat atcagcggtc gaagcggaac aagcttcacc cagatcgaaa
    24061 acgcccgtcg ctccaagcgg gaactgagtt atttagctga ctgggggcaa tcgacagtct
    24121 cttcattgct tgaagcaaat gaagcagaca cttctatgga aatcctagcc tccggaggta
    24181 tccgcaatgc ctatgacata tttaaagccc tttgtctcgg cgcaaatgcc gtgggtactt
    24241 caggaaccgt actgacgcat ctgatgaatc acggcgtaga agaaactatt atactgatga
    24301 agcaatggca agaagagctt cgtctgcttt atactatggt cggcgcaacg aataccgcgg
    24361 ccctacacca acaatctctg atcttttccg gaccagtgaa ggattggtgc gaagcaagag
    24421 ggatcgactt agtgaagtat ggaagacgca cagaaaagag cgtgggacaa aagtaaagaa
    24481 cacttttgtc tcacgctcta aacacgtaca aacggcggaa gtaaaagcaa ctccttcgga
    24541 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaattcctt cttatttctt
    24601 ggagttaaat gcttctgtcc cagcctcttt tttcgaataa atgatgttta atcagctgtc
    24661 cttcggtatt cgttcaatcc gctgatttca caacctctcc actaaacagt gtactaaagc
    24721 tcttttaagg ttgttttccg atataggcaa gtatgcctcc gtccacatac aagacatggc
    24781 cgttaacgaa atcagatgcg ctagaagcca agaagacaga tggtccagcc aaatcgattg
    24841 gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt
    24901 caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata taacctggtc
    24961 caattccatt acattggata ttgtattgac cgtattcaga agcgatgttt ttcgtcagca
    25021 tttttaatcc gccttttgcc gcagcataag cactgaccgt ttcgcgtcct aattcactca
    25081 tcatggagca gatattgatg attttaccgc cgcctttttc gatcatatcc ggaataactg
    25141 cttttgaaac gatgaatggg gcatttaaat ccacgtcgat tacttggcgg aagtctgccg
    25201 catccatttc gatcatcggt gtccgtttga tgatccctgc attgttgact agaatatcaa
    25261 ctgaacctac atcttctttg atctgttgaa ccatcgcttg aaccgcttct tcatcggtca
    25321 catcgcagac ataacctttt gcatcgatgc ctgcttcttt ataatgcgct ataccttcat
    25381 caactgattc ttgattcaag ttattaaata cgatttttgc tcctgctgta gctaaagagc
    25441 gtgcaatctc aaatccaata ccatagacag caccggtgac taatgctact tttccatcta
    25501 aacgaaacat atccatacta aattccatca ttttctctcc cttatgctta ttttaattga
    25561 tccatttgca ccatatccat gtctgtatag gtgatatttt ctccgcacat tgcccagatg
    25621 aatgagtaat tgcttgtacc tacacctgaa tggatcgacc agcttggcga aataatggct
    25681 tgttcattgt ctacaactaa atgttttgtt tcatctggtt tgcccatcat gtggaacaca
    25741 cgtgtatctt cattggcata atcaaagtag acataagctt ccatacgacg ttcatgtgta
    25801 tgacaaggca tcgtattcca agcgcttcct ggttctaaaa tcgtataacc catttgtaat
    25861 tgacagcttt cgcatacatt tggatggatg tattggtaga ttttacgttc attcagtgtc
    25921 aggccttcac cagtctccat tggttttact tggtcgatac tgatttttac atttggatat
    25981 ttatgatgag ctggaacaga actaatatag aatttagctg ggttttcagc atcttgagaa
    26041 gaaaatacaa catgtcgtgt ttcttttccg atatagtatc cgtcttgttt tttcatttct
    26101 tctttttgcc catcgatctc gatgaatcct ggtccaccga tattgatcac acctagttca
    26161 cgacgctcta agaagtaatc aacacctagt tctttgtcta atgtgatttc taattctttt
    26221 tcagtaggtg tcactccacc aaagatcatt cgatcgttgt gcgtataagt caaactgatt
    26281 ttccctggtt caaatacctt ttctactaaa aattcttttc tcaattgttc cgttgaataa
    26341 tgacggatat cttctggact gtgtgtataa cgtgtttcca tttcttgcat tttcttctac
    26401 tccttctatc gaatgatttt tccagagccg catgctaaaa attgtgcgac ttcttcttga
    26461 ctaaattggt tgcagtcccc atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa
    26521 tcaatgattt cttgcggaga catattggat aaaatcccat ggagcacgcc acctgcaaaa
    26581 gcatctccgc cacctactcg atccacgatt ggggtgattc tatggacttg ggattccaca
    26641 tattcatggt tcatccacaa tgtccccttc aattcatttt cacttgctga atgaacggta
    26701 cgcttggtcg aataaaacac ctgaatattc gggaactttt cctgcatttt ttgataataa
    26761 taaattagag gttgttcttc tgcttctggc gctttctcga tacctaacaa ataaagagca
    26821 tccatttttc cagctgagca gatatctact aaaggtaaaa gctgatggat tacttcgctg
    26881 gcttcttgct gtgtccataa ttttccacga tagttgatat cgaagctgat caggcagcca
    26941 gctttttttg cagcttctat cacctgtttc gtcatttttt gccatttttt agacaaagct
    27001 ggggtgattc ctgaaacatg aaatatatct attccttgga ataactcctc tagcgaccat
    27061 tccaaatgat ccatttcagc aaaacttgat ccggcacgat catagatcac tgaggcggcg
    27121 cgttcgccac taccagtttc catataataa gtacctagtc ttgatccacc agataatacc
    27181 tgtttcgtat ctactccata acattgcaag tgctttttga ctgcttttcc caatgcgtta
    27241 tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga aattgctacg
    27301 tttgcttccc ctccgccata atgggcagcg aatcgttcac ttgaatcgat acgggttccc
    27361 tgctctgtgg acaatcgcaa catgatttct ccgagtgtca ctacttttcc cataagcagc
    27421 tacctcttga tttctttcat ttttgctgca tactgttgcg cgacttccgt tactttgtca
    27481 aaatctcctg tagctgcggg tgctaaaagg ttcccaccta ctccgactgt tacgacacca
    27541 gcatcgaacc actctttcat attgtccaga ctcacaccgc cagttggcat gatattgact
    27601 tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc cataagcgct tccaggaaat
    27661 agtttgacga tatctacccc gcttttcagc gctgttttga tctcggtaat agtcatacat
    27721 cctggtaagt aaggaatttg atacagattg cacatctctg ccgtttcttg atcaaaggaa
    27781 gggctaacaa tatactctgc gccagctaag atagccaagc gagctgtgac agcgtctaat
    27841 actgtgcctg cgccaatcac tatttctggt tggtctttgt agaatgacaa aagttctttg
    27901 attacttgat ctgcttgagg gactgtaaaa gtcaactcaa taccagttag accacctttg
    27961 atgattgcgt ggcaagcatt caaagcttct tctttgcttt ttccgcgtac caccgcaatc
    28021 actccggcgt ttttcaatcg agataaaata tcaatttttt tcatcttctc cacctatttc
    28081 ctatttagaa attttatttt ataacttgga aatctatacc catattatac ggcagaaaag
    28141 aaaaattgca aacgcttttt tcattatttt tgaattcttt gctcgatttc gctttttgtt
    28201 tgcttgaggg cactgattat ttgttctttt gtctcatctg ttaaacgata tttcggcata
    28261 cttacactaa atgcaccaat gatttgttcg tttttcacga ttgaggctcc tatacaaaaa
    28321 atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg tttcttttac cgttttgatt
    28381 tcctttttca accgtaatgc attggtgatc gtatattctg tgtaagggat caacggatgc
    28441 ttttcaagat aatcagtgta ctgctcttca ctgaattctg ctaatacggc tttgcccatc
    28501 gcagaattgt ataatggtcg ggtgattccg atttttgaag acatgcggat cgtttgatgt
    28561 tttggatcta gtttgttcac gtacaagatc tcattgttgt ccaatacccc taagtggata
    28621 gtctcatcga tcttttgctg cagctgttct aaaaacggta aagtcaactc taccaaatcg
    28681 atttgttcaa tgtttttatt cgcgtagcga ataagctttg cacctaaacg atagtttttc
    28741 tctgaatttt tatttacgta gccgatcatc aacaaagtat ctagtatttt caagactgta
    28801 gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag agacatcttg gttttccgct
    28861 aaatagtcta gtattttaga ggctttgatc aatacagttc cataaggctt tttattttcc
    28921 atataatatt ctcctatttc tttataagaa aaattatagc ggaaatacca tcaatttaaa
    28981 agagatatcc agatataaac taccatttct ctttacaggc aatttaatgt tgaagatccg
    29041 cttcgttttt acatcaaact ttgatataaa gcaatgattt cttctactgt cgtttctctt
    29101 ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg caattgctgg aatatctgct
    29161 tcttttacac ctaattcaga aatggtttct gggattccaa ccgctttgct caaccgatcg
    29221 atctcaccac aagccgcttc tcttacatct tctaaagaca tttcagctgc tcctttaatc
    29281 cctagaacaa gagcgatctc acggtacttt tctccagtat attctttgtt atatttcatg
    29341 acagttggca aaagtgctgc acacgctacg ccatgaggaa tgttgtacca tgcacttaac
    29401 gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg agaagcccat tcccgcaata
    29461 tactgtccta atgccatttt ttctcgccca ttttgatcgc catctacaga agcttccaaa
    29521 gaatggccga tgatttcgat tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct
    29581 tttgtgataa acccttcaat agcatgcgtc atagcatcca tacctgttga tgcagccaat
    29641 tttttcggca ttcccatcat catatcgctg tcgatgaacg cgacgattgg gatatcatga
    29701 ggatcaacac aaacaaattt tcggtgattt acttcatctg tgatgacgta gttgatcgtt
    29761 acttctgctg ctgttccaga agtcgttggt atagctaata taggtaaaca tggatttttc
    29821 gtatcagcaa caccttctaa actaatgacg tctgaaaact ctggattcgt caaaataatc
    29881 ccaatggctt ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa acagtctgcc
    29941 tctgcctctt taacaaaagc cactccatct tttacatttt ggatcgttgg attaggtacg
    30001 atcccgtcgt ataaagcata agcgattgct gctttatcta gaagttctgt tacttttgtt
    30061 gctataccgt gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc
    30121 tgctgaaatt cagggacgat cgactcaatc gctccttttc caaaatacga tgtttcattt
    30181 aaaatcattc gattactcat tttctattcc tcctaatttg tattattttt cacaataaaa
    30241 acgaaagaac cccaagcaac cggttgccca gttgcttgtt gctacagttc aaagactggc
    30301 atcaattccc aactgactgg agaggcatct ggatttgtct ccatgatatc ttccatgtac
    30361 ttccaccatt tttgattgat agatgtattt gccatttttt cccataactt ttcatcttcg
    30421 atcactaagt aaccaaataa caaacttgta ctcggatcaa gatagatcga atagtcgatg
    30481 accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg tctttttttg
    30541 tattcttctt gtttttctgg gtaaactttc atgcaaaaag cttttttcac catgtcagac
    30601 acctgctttc gtatgaactt cctccaaata acctgctttt ggagtgacac caaacgcatc
    30661 tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag attggtcctt gcgctttgac
    30721 gtacgtatag acttctgctg ccttttctgc tgtctcgatc aaaccaaaga cttcatccat
    30781 gtcttttcct gacccgtaga ttccatgttg tggccataaa accaatcgag tttctttcat
    30841 tttttcagct gttgcttccc caatctcatt tgttcccgga accatccacg gaatgatgga
    30901 aattccttct ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg tacgactaaa
    30961 ggaacgttcg tctaattcat gcgtaaaact catcgctaat agatgactag catggcaatg
    31021 cataacgatc cgattatctg ggtctacttc taaacgggcg atatgactca tgaaatgact
    31081 tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt
    31141 gccatcttca gcaactcgaa cgattccaag attctcggca ggttcgctcg ctacattttt
    31201 aaaatatctt cctgaaccag ttacaataaa atagcatcct gctaaacggc tggcatcaaa
    31261 aatcatcgag atgcggcgga tcactttttc aggatccaag aacggtgtga tctcttcttc
    31321 ttttagtaaa taagaaatat tcccgccatt tctttcatcc catcccaagc gataaagatt
    31381 cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt aaaatatcta tttttttcaa
    31441 tatagccaca ctcctatttt cttaattcca acacgtcttt ttgatattcc tggacttctt
    31501 ccagccaatc caaaccaact ggaacttgat ttttgtaaca gaaataattc catacatcgg
    31561 catacgggaa atctttcaat tcttccgtat aagccattct tgaagtaaag tcaaaagcca
    31621 gctccatttc tttcaatcgc tcaactggtt ccagcatagc tttcagcagt gctttttgtg
    31681 tatttctcgt accgatgacc caagcagcga tacggttgat cgttgcatca aagaaatcca
    31741 gaccgatatt tgttttttcc aacaattcgt tacggactaa ttctttagcg atttcctgca
    31801 attcatcatc catcaaaacc acatggtctg aatcccaacg cactggacgg gaaacatgca
    31861 acatgattcc tttgctgaat aaagctaatg aagataattt attggaaatc acttctgttg
    31921 gatgaaaatg tccagcatct agacaaacaa gtttgtcacg agtcaacccg taacccatat
    31981 aaaattcatg ggaaccgact gtataagctt cagcgcctaa accaaatagt ttgctttcta
    32041 ctgcatcttg tacataccgt tcatctaact cttctgtaaa aatttcatcg agtgattcca
    32101 ttaaacgttc ccgtggggta aaacgatcaa tcggattatc tttcatccca tcaggtaccc
    32161 aaaagttatt gatacttgtt tgcccagttt ctttcccaaa gtactccgcg attttgcggc
    32221 ttcttttccc gtgttcgatc cagtattcac gaacttcttt atctggatga gccaatgtaa
    32281 agccgtcttt catcattgga tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt
    32341 ctttttttgc ccactctacc catttttcaa aatgtttcgg ctcaatttca tttagatcga
    32401 cagattcttc tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg cccggtatca
    32461 acgaataagc cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa
    32521 aattccctgt cgccatgatt ccaccggtca attcgccatc cggtgtcaag aaacctttga
    32581 catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag
    32641 catctgtatc tacaccgata gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa
    32701 tcgtgttcat acgattactc ctttgatttt attcacgtac cattccatcg tgttttgctt
    32761 attttcatct gggtaaaaag tttcacatgg aaacgactgt ttgattacat ttcttgcttg
    32821 ttctatattc gaaaactgac ctgtactgag catttgcatg gcaacattcc cgattgctgt
    32881 ggcttcacta ggtcctgctt ctacaatgat tccggcataa tctgctgtca attgattcag
    32941 tagacgatta ttcgatccgc cccctacgat gtgcagcttt tccaatttcg ttccagttaa
    33001 gtgttctact ttgatcagct cttgtgcgta acaaagagca agattatcat acacacaacg
    33061 agcgatagct cccgatgaga cagggactgg ctgctctgtt tcaaaacagt attgctgaat
    33121 ctcttctatc atatttgggg gattcaaaaa tcgagggtca ttgatttcca tcacttgctg
    33181 cagcggtttt tcttttgcag caagctctgc caattccgca taactgtact cgtactcttg
    33241 cattcgggca acttcctgga tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt
    33301 gttatgggca ccccattcat tcgtataatt ttcagaaaaa gcattgcgag aaacattggc
    33361 gatctttgtt tcaatcccta aaagcgacca tgtcccactg ctgatataac cccaacgttc
    33421 tccttgacct ggcgttccta atacagctga cgccgtatca tgacttgcta ctgtgataaa
    33481 ggtcacatct ggcaaatcat attttgaaaa ttgttctttt tgcagatctc ctagtatcgt
    33541 tcccggctct gtcaatggtg gaaataattc cttcgagaca ccgatttttt caagaagttc
    33601 tatttcccat tcttttgtcc ctgcattcag cagctgcata gtagatgcat tagttttctc
    33661 cgtcaccatg ttaccagtaa agacataccc taaataatca ggtatcagta acaatttatg
    33721 tgcttttgcc aaattttctt tttcttcagc aaacaattgg aacaaggtat tgaaaggctg
    33781 gatctgaatc cctgtctgct catacaactt atctagagaa tagatttcag aaaactgttc
    33841 tactgctcca ttggtacgct tgtcccgata agcaatcgga tcatttatcc gatccccttg
    33901 ttcatcaatc agacagtaat cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat
    33961 ccctgtctgc tttaattttt caaatccaat caaaatctct ttgataagat aatcgatctt
    34021 ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg aaccggtgta cttcttccaa
    34081 gatcaaagaa ccttttgcat ccctcgtact tttcattagt cgtccgcttg atgcaccgat
    34141 atcgatggca acaaatgttg gattcatttc tccacctctt ttactttatt gttgattagc
    34201 actttattaa taaagcgtta tcatttattg acaatgctat cataatacag aatcttatat
    34261 tttgcttcac actattttgg tatataattg cattatcttg acaagttggg agtgtttaca
    34321 tggattactg ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga
    34381 cgcctacaca aagaagcagg aatttatttt tttatatcca agaaatcggt tacttcaaag
    34441 caccaatccc ttactatact gaacgagcac atttaccttc ttacttgata aagtttactt
    34501 tgtctggcaa aggttcgctt acatataatg ataatcagca acagctaaaa gctggagact
    34561 tattttttat tgactgccaa gaatatcagc gttatcaaac gatcagcgaa gaaccatggg
    34621 aaatggattg gatccatttg aatggtcaga acgtccaatc attttatcag gagttcacaa
    34681 aagacggcca taacaccttc cattcaacag gagatccatc agaaaacaag atccaccatt
    34741 tgatcacaga attgatccac atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct
    34801 ctgtagctat ccacgagttg ctgaatgaac tgattttgca aaagtatcag ctagatttcg
    34861 aagaagagga tattcccgcc tacgtttaca aactgaagca atacttagac gaacatttca
    34921 aagaaacgat tacacttgat caattagaac aacaattctt attgaataaa tatcagctga
    34981 gcaaagattt ttccaaattt atcggttctc caccaatcga ttatttgatc agcaaaaaac
    35041 tttcttacgc taaagacctt cttcggtata cagaatttac ggttcgaatg atttccgaag
    35101 aaatagggat tgagaatttt gcttacttta gccgcctgtt caaagcaaaa acaggactat
    35161 cgccgagaga ctttcgtaaa ttcagctgat tcatataata tgtacgtcat tttttcaaaa
    35221 tattttgaaa tatatagata aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac
    35281 agcagctact gcggttattt tttagtgatc attctcattg ttcatcttca aaatgttcat
    35341 aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact
    35401 ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa accactttta ctatatacag
    35461 cactagagct gcggtattct cgcaacaagt gcttggcata gatcagcttt ttcttcatta
    35521 catactgatg aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata tggtatttgc
    35581 tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg atgagataaa ttttttgaga
    35641 tatattgtaa catttgttcc acacgctcat taggaactac agcattctgt tcttgcgcac
    35701 tctcttcttt ttgtacaact tgattgagaa aaatcaagta ttctaccaga gcttgttcat
    35761 attttatgtc ttcaccgtaa atttttttat caggaggatg gtcgataaat gataattttt
    35821 gcttcaatac ttcctgttct atttgcaata cacgactcct cttctctccc attggttgaa
    35881 aacaagccga tagattagac catggcgtgg aacgactttc tagaaaagaa ggggtgacaa
    35941 agatatagac acgctcgaat acatctgaag attggcggac gatacgatgc aaatcattgg
    36001 aatggatgag cagcacactt cctgctttga tcgtaaattg tcgtccgttc aaataaaaag
    36061 tcgcttctcc ctttaatgta gcatggatct catagaaatc atgatgatga tagatcgttt
    36121 tattttggct gacgacatct ttgacatgat agatttcata ctgatcagaa accatcgtat
    36181 tgacttgttc accgctgatt ttcttttgga gcatgtgccg cacctccttt tgttcactta
    36241 tgcacaattt tattatacca cgttttttgt aatcaaaaag agctgctttt atatactctt
    36301 ataaaaaaga agagacgtgg catcttctag ccccgccccc tccattctaa atattagtta
    36361 accaacgtct tctttacatt attcaagaag acttcttcca taacgaatcc catcatcttt
    36421 tgtttcttca agaaccacat acaggtacgg acgattcttc atgatgattt ctttgatttt
    36481 tgtgtactcg atcacaccgt ctcccaaatt taccggcacg attttttcct gttcgacacg
    36541 aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag cttcttctac
    36601 caattggact tgttcttgat aatcactact tgtgatcaaa ttggtcggat ctaaaatgat
    36661 tcctagatag tccgaatcta ctgcttggat cagctgctct acccttgcta aagaatacac
    36721 cggatgattc aaacctggtt ctatccccac catcatttgg tatttttctc cagcttttac
    36781 caagcgacgg ataacggaca cagcttcagc aaatgcttca tctgtgaaat tttcttccgt
    36841 atattggatt tccggcaaaa cacatccagt ttccgaagca accatcgacg caccaaagga
    36901 agaagcatag cgaagatact gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc
    36961 tggatgaatc atattgatat agcaacttaa aattcctacg gtcacttctt gcttttctaa
    37021 aactcgtttg acataatttc ccatcccaga attgatctca tttgcgccag aaggtatttc
    37081 aggaaacgaa agacctaagg cgagctgcag tgtatgaatc ccttgttctt ttgttttttc
    37141 tgcaagatct tctatcgttt ggacttcgga aagatcatgt cctctaattg taatgttcaa
    37201 cataactatt ctcctaactc agcatattgg gtagccacat gacgatttgc gggaagtaag
    37261 taatcaaaaa caagacgact aagataaccc cataaaaagg aagaagcggt ttcagcattg
    37321 cctctgcttt tacgcctcct acacttgcac cgacatatag tcctgtacct actggcggcg
    37381 tgatggagcc tacacacaga ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc
    37441 ctacacttgt gacgattggt aaaaatattg gtgtaaaaat caagatagca ggcgccacat
    37501 ccataaacat acccaccagt aataacacaa gattgactaa taacagaata ataaatttat
    37561 tatccgttac gcccataatc aacgaactaa ttgcctgagg aataccggta aacgccatgg
    37621 cgaaagacat catggaagaa gtagccaata agaacatgat cgtccctgac attgctactg
    37681 attgaaacaa catattaggt atatctttta gttttaccgt tttatagtaa aacatagcaa
    37741 tcaacaggga gtaaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca
    37801 agataccacc gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt
    37861 ttccagcatg agccatctgt cctttttcga caacagggta tttattcttt ttagcaacga
    37921 tgaatgctac tagcatgatt cctagcgccc acaaagcgcc tacgagataa cctcccataa
    37981 acaaagcaga gatcgaagca ccaccagcaa caagtgaata catgataaaa gcagtacttg
    38041 gaggaatgac catacctgtc ggagctgacg cgatattgac cgctgtcgca aacttccggt
    38101 catatccttc ttccacttgc tgagggatca acactccgcc aatcgcggta gaagcggcaa
    38161 tggccgagct agagatggaa ccaaataaag cattccctaa cacattcgta tgagccaaag
    38221 cccccggcac gcgcccgacg aacaacatcg caaaatccac tagtttctta gctatccctc
    38281 cattattcat gataatgccc gagagaacga aaaacggaat agcgatcagc gaaaaactat
    38341 tgatacttcc caccattttt tgagctgaac taaaagcaga aacatcaaac ggtataacta
    38401 acaataaagt catcatcgat gagaatgcaa tacttatagc aatcggcatc cctaatacaa
    38461 gcaacacaac aaatacaaaa aataacacta atccggcttc tattgccatt ttattcctcc
    38521 atttttatat tttttcttga attgctggct tgttgcttaa tgtatcgatt agattgatca
    38581 ggctatagat aatgatgagt acccccgata caggtactga cagataaaca tatcccatag
    38641 gaatacttaa agaaggcgag atctgcccca ttgtcaatgt gacagcattc catccgccat
    38701 acaccatgat catcgcagca aataccagaa ataaactttg aacaacaact tctaaaattt
    38761 tacttttttt gccagttaat ttttcactta aaaccgttac ggctaaatgt gctttttgtc
    38821 ccacaacata cgcggaagac aacatggcaa accagacaag accgaatcga acaagctctt
    38881 ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa tacttgccat aaaacaacag
    38941 cgaccatccc aacaagcaaa acagctccca ggatctctaa taccttgttt aaaatcattt
    39001 tcatcacagc tacgctcctt gctcttggat cttttgataa aggtctttgt aggtaggatc
    39061 gtttttgaat atatcatgaa gtggggcaac tgcctctcga aacggtgtat tatctacatc
    39121 attgaattgg actccatgtt cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc
    39181 aaaagcttca atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg ctttttcctg
    39241 ctcatcagtt agctgttctt tgatggcatc attcatgatg atgatatctg gcacacgtgt
    39301 atgctcatca taggaatagt atttagccac accaccatgt ccagctgtat acaagacaaa
    39361 ttcattgttt tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca cttcatcact
    39421 tcccataggt acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg
    39481 catcacacgg atcttctttc ccttcaaatc ttccggcgta tggatcggac cgtctaccat
    39541 ataaaagctg cgctgaccag aatcataata ggtcaatccg gtaaatccta aatttttggt
    39601 cgattggtag attggctcca ttatttcttc attatccatc accttaaaga aatgttcttc
    39661 atcattgaat agataaggaa ctgaaaaaat cgaataatct ttagagaaac tttccaacgc
    39721 tgaggcactt acttttgtga aatctactgc tcctgactgt gtcaattcga tcagttcacg
    39781 ttctccaccc agttgtccat ctgggaaata ctggatctgt acggaaccat ctgttttttc
    39841 ttttactaaa cgcccaaatt cattcattgc atcaattact ggctggctgt tgcttgcata
    39901 agcaaagcgc aaggtaacta tctcatttac atctgcatct gcgacagttg ccacactaga
    39961 attatttccg cagccgctta gacctagcag acctaggcta agcgaacttg ctgcaaacag
    40021 cagtgatgtt tttttcatat gggtttcctt tcttttaaca ataagagacg agcaattctg
    40081 tcaaacaaag caccgttaac gactgtccat aaggcattgc tgtgattccg atattccgat
    40141 aaaaatctaa actgtccccc attcctgtcc cgattgaaac gttttgaact tctccttttt
    40201 catcgatttg ttcaagcaat ccttggatag cacgataagc gacttcttta tattcttgtg
    40261 gtaaataacg tttatggacc gctttcaaaa taccgtaagc aaatcctgca gttgcagaag
    40321 attctacgta agaagaagga tcatccaaca acgtatgcca taaaccgctt tcagactgat
    40381 aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat caataatttg cgcaatgcat
    40441 cttctttcga taattctaag atttcaatga tctctggaat agcaatcgtc aaccagcagt
    40501 ttccacgagc ccaaaatgct tcggcatagt tatgatttcc ttcaaacgtc cagccgtgat
    40561 accacaatcc tgttcgttta tccatcaagt atttgatatg gatcataaac tggtaacgag
    40621 cttcttctaa atattctggg cgattcaaca gtttcccgat ttttgccaaa ggaagcactg
    40681 tcatcatcaa tgtatcgtcc cacaactgat tcttattttc cggtccatat gtggcatgct
    40741 gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac ccattctgcc cattgttcta
    40801 aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa gtaagccata gtcaatagcg
    40861 gtgccattgt attgacgttt ttgggcggag cgccttcttc catacgatcc tcaaaccatt
    40921 cattgaccac ttgataagcg cgaggatttt ttgtcatctg gtaattttta taaataccat
    40981 atagaccgac accttgtggc caattccaca ctgtccagct tttgtcatct actttcagtc
    41041 catcaaagtc caacaaaaat tcaccattat cgtctttgat cgttgtcagg ttttcaacga
    41101 gcaggtcaat tttttctttc actaattctt taggtacaaa aatctcttct ttaataatca
    41161 tctttacgcc acctttttaa acgctttcat tttatatcct taatatagca gtaataccgt
    41221 ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa ttgacctaaa tgttaaataa
    41281 caaaagtaac tttcagttac ttggaaatta aaaaaagaac ttggagaaac aacgttttcc
    41341 aagttctttt tcatctattt tcttatcttt tttctacgct ttcttttgct tatccgttgt
    41401 aagcatcaat gatgatctgt ttcaattcag agatcaatgg ttctttcgga ttagctgtcg
    41461 tacattggtc ttcgtaagct aattcagcca tacggtctac ggttgtatct agtgtttctt
    41521 gtgtgactcc ttgagctttt agactcatgt tgatgccgac agattttcct aattcataaa
    41581 cagccgttgc taaagcttcg actaattcct ctgttgtttc gcctttcaga cctaagaatt
    41641 ttgcgatatc tgcgtaatct gtatctgcac ggaaatagtc atatttaggg aacatcgcat
    41701 gtttttgtgg atcttttgca ttatagcgga taatatgcgg taaaagaatc gcatttgtcc
    41761 gtccatgagg gatcccgtat tccccgccga ttttatgtgc gactgagtga caaataccta
    41821 ggaatgcgtt agcaaacgcc ataccagcca ttgtagaagc gttgtgcatt ttttcacgtg
    41881 attccatatc aggtgttttt actgattttt ctaaatagtc aaagactaac ttgattgctt
    41941 gtaggctcaa tccacgtgta taatctgaag ccatgactga aacataggat tcgattgcat
    42001 gtgttaacac atccatcccg gtatctgctg tgacagatgc tggaactgac atgacgaatt
    42061 gaggatcgac gattgcgaca tctggcgtca atgcataatc tgctaacgga tatttcacat
    42121 gtgtttcact gtcagtgatg accgcaaatg gcgttacttc agaaccagtc cctgatgttg
    42181 tcgggataca tacgaattgt gttttttctg gtttgtcgat cttatatgtc cgtttacgga
    42241 tatccaagaa tttttgtttt gcaccaaaga aagatgtgtc tgggtgttca tagaacatcc
    42301 acattccctt agctgcgtcc attgcagaac cgcctccaag agcgattact gtatctggct
    42361 tgaagtctgc catcagtttc gttcctgcgt atacggtatt tgtcgatggg ttcggttcaa
    42421 catctgagaa gacttcgatc ttcacatcat ttttacgtcg tgccaatact tcacgaactg
    42481 tatcgcagta accgaattgg accataccag ggtcacaaac gatcatgaca cgttcaacgt
    42541 tttccatttt ctctaagtac aatagagagt tcttttcaaa gaagattttt ggcggtaatt
    42601 tgaaccattg catgttattt ctccgtttcg ctacagtttt gacattgatt aagtttacag
    42661 cagagacgtt acgagaaaca gagttcttac cataagaacc gcatcccaaa gtcaatgacg
    42721 gaatcatttc gttatagata tcaccgatgc cgccttctgc agatggtgta ttcactaaaa
    42781 tacgacaagc tttcattcga agaccgaatt tgacatgcaa ctcttcgttt tctgaatgga
    42841 taactgctgt atgtcctaat cctccaagat tcaacatacc ttcacacaaa tcaaacgcat
    42901 ggtccgtgtt gttagctttg atcatggcaa gaactggaga aagtttttct cttgatagcg
    42961 gataatctgg gcctacgcct tccaattcag caatcagcat tttcgttcct tttggtacac
    43021 tgattcctgc caattttgcg atttccatcg cagaatgtcc aacgattgat ggattgaccg
    43081 catattttcc ttcgttcatc actgcatctt ctaatttttg caattcatct ggttttacga
    43141 tataaacttg gtgtgcttgg aattctgctt ttactgctgc atagatttct ttatccacga
    43201 tcacagcttg ttcagaagca caaatcattc cattatcaaa tgttttcgaa acgatcaaat
    43261 cgttcactgc acgtttaatt tttgctgttt tttcaatata agctggtacg ttacctggac
    43321 ctacacctaa agctggtttt cctgtagagt aagcagactt gaccattcca gctccacctg
    43381 tcgctaagac gatcgcgata cctggatggt tcattagcat agaagtcgct tcgatcgaag
    43441 gatgttcgat ccattggata caattttcag gagctccggc agcgatagca gcatcacgta
    43501 ccactcgtgc tgcttctgca gaagattttt gggcacttgg atggaaagca aaaacgattg
    43561 gatttcttgt tttcaacgcg atcattgatt tgaaaatcgt tgtggaagtc ggattcgttg
    43621 ttggagttac tccgcaaacc acaccgactg gttcagcaat ttcaatcaac cctttttgga
    43681 catcttcatt gatcacacca actgttttat catgtttgat attatgccaa atatattcgg
    43741 acgcatacat atttttgatt gctttatctt cgtaaacacc gcggcctgtt tcctctacag
    43801 ccatcttcgc taatggcata tgttgatcca aagctgccat tgccatttca tgcacgatat
    43861 ggtctacttt ttcttgatca aagctttcca tttctttcaa tgctgcattt gctttttctg
    43921 ccaaatcatc aatcattgct tgtacgtcaa ttgtcttcgt tttttctttt tctattactt
    43981 tagccatcat tatcctccta atgggtcatg gttatttgtt aaataattca catgtatatc
    44041 atacaatatt tatttttcta tgtaaacact tttttgtgat ttttttcaca gttattttta
    44101 aatataaaat tgctttattt acaggtgttt gtatagaaaa atatataaag ttatttatta
    44161 ttatttatat gacacacccg attattcaat attgagcaat atatcccatg ctcttatctc
    44221 tttctttttt gaataaaaaa tgaggtagga catctttctg ccctacctcg tctttaaata
    44281 agctttgata cttatttttc ttccttctct tctgtctctt ccagcgtttc tgttgtttca
    44341 gtttttactt caactgtctc agctggttct tctgttgttt caacagtagt cgttgcatca
    44401 tttgttacga cagtccctgg tttgaccgtt ttgattgctg cacgatcgta ctcaaggaag
    44461 atcccttcgc agtctaaaac aactgtccgt ttgtcattgt cgatttctga aacaactcca
    44521 tgtaatccgc cgatcgttac gactgaatca cctggtttca tgctatctaa caagttttga
    44581 cgttcttgct gttgtttctt ttgcgaacgt gacataaaaa accacattgc taatagcgcg
    44641 ataaacatca caagcattgg taatgttccc atttttttca cctcactaat tctgtatcta
    44701 caaaaaacac tttagcagaa aaatgctgat aacactagtt ttttacttaa tttttaaaag
    44761 attttccttt agaagctttt tgcgttttct ttattgaacc cgtattcttc aaaaaaggct
    44821 tctctaaatt caagcaaatt atcatccatg attgcttgac gtacttgttt cattagattc
    44881 aacaagaagt ataaattgtg gtaagaagtc aaacgaatcc caaatgtttc atcacatttg
    44941 atcaaatggc ggatataggc acgtgtatag tttttacatg tataacaatc acatttctca
    45001 tcaattggac ggaaatcatg ggcatatttc gcatttttca ctactagacg gccttgagaa
    45061 gtcatacatg tgccatttcg agcgattcga gtaggcaaga cacagtcaaa catgtctact
    45121 ccacggataa caccatcgat caatgaatca gcagcaccga cacccatcaa gtaacgtggt
    45181 ttgttttcgg ggatcaacgg tgtagtgaat tctaatacac gattcatttc tgatttaggt
    45241 tccccgacag ataacccgcc gattgagtaa ccagggaaat ccatcccgac tagatctttc
    45301 gcgctttgac gacgaagatc ttcaaaccct gctccttgga tgattccaaa taatccttga
    45361 cggtctggat tagcatgagc tttcaatcct cgttctgccc atcgagacgt acgttctact
    45421 gattttttca cgtagtcgta actttcatcg aatggtggac attcgtcaaa actcatcatg
    45481 atatcagagc ccaatttatt ttggatgttg attgcttttt ctggtgataa aaacatacgc
    45541 gaaccgttca agtgattttt gaaatgcacg ccttcttctt cgatattccg catatcactt
    45601 aatgaaaaaa cttgaaagcc gcctgaatct gttaaaatcg gttgatccca gttcataaat
    45661 ttatgcagtc cgccagcttc ttctactaag tcttcaccag ggcgtagcca taaatgatat
    45721 gtattactta aaataacgcc agcacccatt tctttcaact cttctgggga cattgttttt
    45781 actgtagcta atgttcctac tggcatgaac attggtgtgg ggaaagttcc gtgtggcgtg
    45841 atcaattccc ctaaacgcgc gccagtatgt ttttctttct tgattaaacg atagcgaatg
    45901 gcaggttgcg tcattcattt ccctactttc tatataaaat atcttgacaa ctcccaccat
    45961 cataatcgtt tcaagtgctt tttgcaagtt ccctcggctt ccttctactt tatttaatat
    46021 tctttttttt atcctttttt tatgaataac aaaaaaaaga aaaacattat gacagaaata
    46081 ttttttgata catggtatat tggtgttatt aaagaaaagg agggaaaaat catgcaatct
    46141 tctgcagaat taaaacgcca agcaaaagag agtctaaaag gacgttgggg acaagctgtt
    46201 ttactaaatc taatccctac attgattggt attgctacta ttcttcttat tatgattcct
    46261 attatcttgt ttgccaccac catatatgat ggatcagcga gcgtctcaga agcgacaact
    46321 tcaggaggcg gtagttcagg aggcggtggc ggaattattt ccagcatcat tagcgctttg
    46381 ttcatgagcg gtatttcttg gacttatctt gatattttac gtggacaaaa agatcagatc
    46441 gaaccattca aagatgcatt tcgtggattc aaaggtttgt ttatcggcgg agtgatcagc
    46501 ttagctgtgc tgatctcgat ctttacaaca ttatggggtt tgctattagt tattcctgga
    46561 atcattaaaa gctattctta ttctcaaagc tattttattt actacgatat cgtccaagaa
    46621 acaggacaaa cacctaaagt actggatacg atcactgcaa gtcgaaaatt gatggatggt
    46681 tataaaggac aacttttctg gttagatctt tcatttatcg gctggcatat cttagcatta
    46741 gcaactgctg gtattggtta tctatggctc agcccataca tttctgcaac aaaagcagca
    46801 ttttatgaac aattgccaaa agatatttaa aagagagtgt gacttgtgac cgagggggtg
    46861 gcaggagaac accgtttatc gagaggcagt aaaagaaaca ttcagcccat tagcaaaaag
    46921 gaggtgtgac aacattcatg tcacacctcc tttttgtata gttccagtaa aaagaaaatc
    46981 aacttagaaa cagttaatca ttcctcttct tctgcttccg gtaatcgatg gaacttgatc
    47041 agttcattca ccgcgattgg tacgatagat aaaatcccta ccaagaacca gtgattcaaa
    47101 ctgatgtgta ctaggccgaa tagttcttgc gtaaatggca gcaaagcaac agccgttgta
    47161 atagctaatg ccagtaacgc catttctacc aaagctttat tggtcgctaa attgactttg
    47221 aagatcgatt ggctgctgcg tacattgaac acatgcagaa tcgatgaata agctaagatc
    47281 aagaatgcaa cagtttgacc gacatgctga cttgcgtcta cccatgggct gatagtatcg
    47341 acataactac ctaaataata accaaacaag gttacgatgg tgaagacccc tgcatttatg
    47401 gcaatttttt gcaataaacc tcgagcaaag atcccttcat ttttcggaat gggtggctga
    47461 tccatgatgc cgtgttccgc tggctctctt cctaaagcaa atcctggaag tccatcagca
    47521 actacattga caaacagtag ttgcacagct gtaaatggtg ctccccatcc taacagcatc
    47581 gcaatcaaaa caataaagat ctccgaaata ttacagctta gcaaaaagtt gatagcttta
    47641 cgaatattct gataaaccga acgtccttga ctgacagcgt ctacgatcgt cgcaaagttg
    47701 tcatccgtca aaatcatatc tgcggcactt tgcgccacat ctgttcctgt gatccccatg
    47761 gcacacccaa cgttacttgc tttcaacgct ggtgcatcat tcacaccatc cccagtcatc
    47821 gctacgactg cacccgtacg ttgccaagat ttgacgatcc ggatcttgtc ttctggcgtt
    47881 acccgagcat agacagagag agctttgaca cgcgcatcta attcttcgtc tgataattga
    47941 tgcagttctg atcctgaaag tgcttctgac ggatcattca agatacctaa ttctttcgct
    48001 atggcactgg cagtcaccac atgatctcca gtgatcatga ctgttttgat gccagccttt
    48061 ttcgctcgct tgattgctcc tttactttct ggacgtggtg gatcgatcat tccgatcaga
    48121 ccaatcaaac gtaagtcttt ttccagtgct tcagaagaaa tatcttgcgg ttcttcgtca
    48181 taagtagcat aaccaacagc aatcactctc agtgcccgtt taccaaagcg atcattaacg
    48241 atcgccgctt gttctacatc gcctgaacta aagcgcggag caagaacatc gaatgctcct
    48301 ttggtgattg agatgtattt ttcgcccatt tggtgaactg ttgtcatcat ttttcgttca
    48361 gaatcaaaag gaagctcgtt tactcgtgga tatttttctt ctaattctgc ctttgtatga
    48421 taattctctt caacagccct tacgatggct acttctgttg gattaccttt gataacaagg
    48481 tcgtcatctt cttgctcgac cgtcacatct gtacatagtg cagccatttt caatatttcc
    48541 atcgcgctgt tcgtcattga atcttcgata ttcgttactt catcttcttt gctccatacc
    48601 cggcggacgc gcatttcatt ttgtgtcagc gttcctgttt tgtctgagca gataacatta
    48661 gcggttccta gcgtttcaac tgctggcaac tgtcggatga tcgcatgttt cctcgccatc
    48721 ttctgcacac cgtatgcaag tgtcaacgta acaataacag tcaaggtttc cggtacagct
    48781 gcaacagcca aggaaatcga agtcatgaac atctcaagga gtggttctcc ttgcagttca
    48841 ccaatcacaa atactaaagc cgcagccgct aaagcgatca tactgattcg tttgcctagc
    48901 tgattcaatc ggacctgtaa aggtgttttt tgcatagtat tttcgttcaa aagatccgcg
    48961 atcttaccca tttctgtagc catgccaatg gcagtgacga tcgctttccc acgacctgca
    49021 gcaaccgtac atcctttaaa taccatatta agttggtcac ctaatgactc atcttcttca
    49081 gcaataaatg ctgcatcttt ttcaactgct tcactttctc cagtcaacgc cgattcttct
    49141 acacgtaatt gtgatgcttc gatcaaacgg gcatcggcag aaatagcatc accggactct
    49201 aaaacaagaa tatcacccac taccagttgc tctgactcaa cagtggtcac tttgccgtct
    49261 cgtatgacag ttgccgtttg tttattcata tcttgcaagg attccagtgc tttttctgcg
    49321 tttccttctt ggacaatcgc caataccgag ttgatcacaa cgatagcgat gatcagcagt
    49381 ccttcaaata aatccccatg ttctgtagca aatgcggtat aaaaggaaat caccgctgct
    49441 acaagaagga tgatcgttgt aaaatcggac aagcttcgaa agaatttttt gatcatcgat
    49501 tctttcggcg cctcatcgaa tttattcaat ccctgctttt ttcgccgttc atccacttct
    49561 tgtgaagaca acccagtatc tggatctgct tctgttacct tctttacttc ttctattgtc
    49621 tgcttgtatt cttccatcct attcctcctt taaaatcttc gtacaaaaaa aggtacttat
    49681 tttactataa catccttttt ttatttactt caaataaagg gcttatcgat tatttgttaa
    49741 gaaagcgtaa aatttaaagt gcttatattt gcaaacgcta ccaaaggatg ctattatata
    49801 gatgtaagga aggaggatac catcaaatag acagaagaat ttctcgatag aacagtgtaa
    49861 gcaaacaaag ttgttagaag tacaggtttc acccagacat gcgtgtagag tacaaaccta
    49921 taaatagtga atgcgtatgg atgtttggat cacgaagtat ccgaaacaag aagtaatgcg
    49981 atacgtaaca attgaataac gtaaacgtct gaaaattgaa taggaacaag agatgtttca
    50041 aatgttgaag atgtaacgaa aacaaaattt ttgtttaaca tgaagaaatt caacaccgtg
    50101 ctttgcatgt atgatggaaa gttacaatca tttctgttta tttgaatcct tccgaggtac
    50161 aagaaagccg agcttatcga gagctcggct ttttctttgt cataaaatct ttgtaaagct
    50221 tttttgttat tcaaaactag cggcttttca taaccttatt tcacgaacat cgcatctcca
    50281 aaactaaaga aacgatagcg ctcttcaatc gcatgctggt aagcagataa agtcaattct
    50341 cttcctgcaa aggcactaac taacatgact aaagtcgatt ttggcaaatg gaaattagtt
    50401 gagaaagctt ctaccacttt gaattcatag ccaggcgtaa tgaaaatatc agtccatccg
    50461 ctgtctgctt tgatttctcc attaaatttc gttccaatcg tttctaatgt tcgaattgaa
    50521 gttgttccga cagccacgat tttcccgcca tttttacgta cttggttcaa tcgctcagcc
    50581 gcttcttccg tcaaacggta gaattcacta tgcatatggt gttcttcaat tttatctacg
    50641 ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca gatagactaa ttcaacaccc
    50701 tttgcttcga tctcatcaag taattctttc gtaaaatgca atccagctgt tggcgctgca
    50761 gcagaaccat tttctttcgc atagacagtt tgatagcgat ctgggtcttc cagacgttct
    50821 ttgatatatg gaggcaatgg catttctccc aatgattcaa gaatctctag aaaaattcct
    50881 tcatactcaa agtcaatgat ccgtccgccg tgttccaatt cttctttcac tactgcttgc
    50941 aatctgccat caccaaaaga gattcttgtc ccaactttgg cacgtttcgc aggtttgatc
    51001 aatgtctccc acgtatctcc ttgtgtattc gttagaagaa ggacttctaa atgcgcacca
    51061 gtatctggtt tttctccata taaacgagca ggcagaactc gtgtattatt cataactagc
    51121 gcatcaccag gctgtaactc ttcaatgatt tcatggaaat gtttatcttc catctctcca
    51181 gttttccgat ccaaaacaag taaacgagaa tgatcgcggt cttttaatgg tgtttgtgcg
    51241 attaattctt ccggcaagtc aaaatcaaaa tcttctgttg ttaacatatc gtcactcact
    51301 ttctatcatg tgcttcatca ttctatcatg tttttcgttc tgttggcaaa atcagcactt
    51361 tttcacttct attttttgtt ttcctaaata aaaaaactac cctatttgta ttttttcatt
    51421 gttttaggca tactagtaat agatagttct tcattgaaaa tagaaaaaga ggtggcacca
    51481 tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg aaaagtcgct gtagaggacg
    51541 tcaatctttc atttgaaaag ggtgagttca tttgttttat cggtacgagc ggaagcggaa
    51601 aaacgacgac gatgcggatg atcaaccgaa tgactgatcc aacacaaggg aaaattttga
    51661 ttgatggaaa atccatccaa gaaattgatc cagtggaatt gcgacgtcag atcggctatg
    51721 ttatccaaag tattggattg atgcctcaca tgacgatccg tgaaaatatc actcttgtcc
    51781 aaaaattgtt gaaagtcagt caagaagaac ggaataaaac agctgaaaaa atgattgatc
    51841 ttgtggaatt gccccgtgag atgttagatc gctacccaca tgaattatct ggtggtcagc
    51901 agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca agatatcatc ttgatggacg
    51961 agccattcgg tgcgcttgat ccaatcactc gtgattcctt gcaagacctt gtcaaagatt
    52021 tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca tgacatggat gaagcgatta
    52081 aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt gatccaattt gatacaccag
    52141 aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga gttgattgga gaagatcgcc
    52201 tactacaagc caagccaaat gcgacaagag tcggcgaagt tatgctaaat acagcaatca
    52261 cgattacacc ggaaaaatca ttacaagaag caatcaaact gatgagggaa aaacgtgtag
    52321 atacgttgtt agttaccgat aattcgaatg tattaaaagg atttatcgat gtagaaacgt
    52381 tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat tttgaataaa catgtttttt
    52441 acgtaaaaga atccgcctat cttcgtgata ccttgcaacg cattttaaaa cgcgggctga
    52501 aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg tatcctgact cgtgcatcat
    52561 tagtggatat cgtctacgat gtgatttggg gagaagatcc cgcaactact gatgttgcag
    52621 agtcagctac tcctgaagaa tctaaggagg tctgacctat gcagcagttt tttcatcagc
    52681 acggttcaga aattctctca aaaggttttg aacatcttta tatttcagga cttgctttga
    52741 tcattggtat catcattgct gtaccgcttg gtgtcttact gacacgtttt ccaaaagctg
    52801 ccacgattgt gattggctta accagtgcct tgcagacaat cccttctctt gctttattgg
    52861 cattgatgat cccgctattt ggcgtaggta aattacctgc gattatcgca ctctttattt
    52921 attcattatt gccgatttta cgtaatacgt acattgggat gaaaaatgtc gattggaatt
    52981 atcgggatgt ggcaaaaggc atggggatga ctgagttcca atccattttt tccgttgaat
    53041 tgcccattgc tatgccaacg atcatggctg gtattcgttt agctgccgtt tacgtgattg
    53101 catgggctac acttgcctct tatatcggcg ctggtggttt aggcgatttg atttttagtg
    53161 gactgaataa ttaccaacca gatctcattt tggcaggtac gatccctgta acaattttag
    53221 cactattggc tgattggctt ctaggtctct tggaacaccg gctgacaccg atcgcactta
    53281 gagaggagaa tgaagaatga aacgtatact aaaaatgacc atggttcttt tctctcttct
    53341 tatacttgcg agttgttcct tccctggtct tgccagcaat acagatgaag acaccatttc
    53401 gatcacaggt ggaatcactt cagaagcaca gatcttagca agtctagttg ccggaatggt
    53461 tgaacattat acagataaaa ataccgcaat catcaacaat ctggctacta caactatcaa
    53521 ccatcaagcg atgatgaacg gagatgcttc aatttcagcg gctcgctaca caggaacaga
    53581 tttaacgact acattgaacc ttcctcctga aaaagatccg aaaaaagcgt ttgctaccgt
    53641 gaaagatgaa tttgagaaaa gatacggtca aacgtggttc ccgtcctata gttttgaaaa
    53701 tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa taccatctat ctaaagtcag
    53761 cgatctgaaa aatgtagcgg atgaattagt agctggtgta gatacttctt ggatcaatcg
    53821 taaaggagac ggttatgacg gcttccaaga aacttatggc ttctccttca attcgatatt
    53881 acctatgcag attggacttg tttatgacgc agtcgaagca ggaaaaatgg atattgttct
    53941 tggctactct acggatggac ggatcgccag ttatgatcta gtcatgttgg aagatgatcg
    54001 tcaattcttc ccaccatatg atgctgctcc agttgtagac aacaagatat tagctgatac
    54061 gcctcattta aaagaagcct taaataagtt aggtcatacc atcagtacgg aaaaaatgca
    54121 ggaattgaac tacgaagcag ataataatct agtggaacca tcagtagtag cacaacgttt
    54181 cttacaagaa aaccactatt ttgaagggaa gtgaagcaat tggataaaat gaatgtcttt
    54241 caacaatttt tctattattt ccaggaaaat ggctcctata tctttgcaca atttatccgt
    54301 cacttcctga tttctatcta tggtgtcctc tttgcagcag ttgtcgggat tccggttggg
    54361 atcatgattt ctaggagacg taaattagca aactgggtga ttcgtttggc gaatatcatc
    54421 cagacgatcc cttctctagc catgatctcc attttaatca ttggattagg cctgggagtc
    54481 aatgtcgtga ttgttactgt atttttgtat tccctcttac cgatcatcaa gaatacgtat
    54541 accgggatga tccaagtaga caaaaacatc cttgatgtcg gcaaaggcat ggggatgact
    54601 gctagacaac gactattcat ggtagagtta ccactctctg tttctgttat catggcaggc
    54661 ataagaaacg cgttagttgt tgccatcggg ataaccgcga tcggtgcatt cgtcggtgct
    54721 ggtggtcttg gagatatcat tatccgagga accaatgcaa cagagggcac atcgattatt
    54781 ttagcaggtg cattgccaac tgcattaatg gcgatcatca cggactggct tttaggtatt
    54841 ttggaaagac ggctagatcc agccagccgt acatcacgct gatgttattg taaaaaacaa
    54901 aagcttctgc gaccatttgt tggtcgcaaa ggctttttta gttctgtcat ttttaaggca
    54961 ataatccact ctgattcttc cctccttatg ggcaaagagt tgcttttctt agctatttat
    55021 ccattccgta tactccaact tcatactgcc aacgggttca tatctgcatc caccgactaa
    55081 gtttttttct tatccgctaa ggcattttgc caatccattt tcccatcact cgtcatggaa
    55141 ttcataccac atgatctcta aatactcgtc tgcttgttct ttgaattggg catcaattct
    55201 tacatccttt ggcgtggctt tcatttaagc cttttccatc agtcctcgat tgttcgatgc
    55261 cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt aacttcgatc atcttttata
    55321 caatcgggaa cttgccatcc tcatccttgg ctaaaaacat atctagccat tccagcttct
    55381 cgccatggga ggtttgcggt tcctaaaacc ggtacacgat tcttatggac tgcatccgtc
    55441 gcaactagac ttggcgatac gatgatcctt caccagaaga gcacccccgt aaaccaattg
    55501 atcgatatat tgccaataag aaaagacatt ggcgtaaaag atatcgatcc ctcgtgatgc
    55561 attgccgttg atactgctat tcatggccga aagcgccacg actttaactt cttccgtttg
    55621 agtgacattc atgttcggca atgtcgcttt ggatacccgc tctgctaaag gaacgacact
    55681 ggtattgtat ttcgtatctg tatcttttcc aaaagaccat gccaacgaat cctctgggaa
    55741 ccaataagac gactccggtt gattgtccat tgcttttttc aacatacttt tggctgtttc
    55801 tcgataaacg acatttccaa ttgtcttcgc ctcactttgg cgataatcga tggtcgttgc
    55861 cactgccaac actaacaacg atgctccaat tttactgatt ttccttgtca cacttcttcc
    55921 tccttgtttt acataaaccc gtcaacattc cattcctaaa ctcaaacaat gacccttcaa
    55981 aggtggagat ataggaacaa attaaagaaa tcatgcaata aatccaagaa ggttggtttt
    56041 agaacaaaaa aagagtctcc tttttgggga gactctttgt taactatcta aaaaggttgg
    56101 ctcttctcac ttcactcata atgtgtccag gcagaataaa tttctacaac tttattttcc
    56161 tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc tgcgtgagta tctgccttca
    56221 tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat atggatcttc cttcaatgtc
    56281 tgaatcactt cttcaaattg tttctttaaa ttcgtttgct tgatttttct taaatccact
    56341 ttcgcagagt ttttgattgc gactgtatag ttactcatca aagagcttcc caatcaatat
    56401 catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc tcgtacttta tctaaagtac
    56461 ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca gtcttccagc ccaatcaata
    56521 cagcattgtt tccactacga tcactaataa tctcgatttc agtatgattt tcatttacct
    56581 ctttcaataa ctgataaaaa tttttacgag cagtgctagg attcatcgtt gtattagcca
    56641 ttgtctctcc tcctttttct gatataatta tagcgtactt gattaagtac gtcaaaaaaa
    56701 tacaataaag cacttaaatt cagcaaactt ttcctctttt ttacatagag aaaaagaccc
    56761 ctttttaggg aggatctttg tccacgctca cttcttactc ctttacgact agcggtagcc
    56821 aaacgtaaag taattggtaa caacgtatca tctttagcaa aggtcgaatc caaagcctgt
    56881 gagtctatgc aacaacacca atacacgaag gttcctcgtt gcagtgtgac ttgattgata
    56941 tcttgaataa cctgaggata cgcacgaaag atttgtaacg gttattcaaa actgattttg
    57001 gccgtcaagt cttctactgc ctcaaaaatg attgcctctt tcgtctcctg tcattcatgt
    57061 tcactatcga tttgatactt gcttagaccg ttgggtttgt gaatcaccaa ttaaaaatgg
    57121 cactgagtta tggtaacaaa aaatggtcac ttgacgaggc tgagattcct acttatgaag
    57181 acacatggca cttaagagtc gaggtaaagg aagaagctga aaatatagga ggctgtacat
    57241 tatttggcaa aggattcgga aattatgatc aagacatcaa aatcaaaatg ttttacagct
    57301 aaataggaag aataataaca tttcatacgc tgttcccatc tttaatggct tatagagggc
    57361 acctttagct gagaataaga caaaacagac cctctagctt caattaagag ctagagggtc
    57421 tgtttttgat tttaaaactt ggtttatttc ttcaaatggt agctataggt gctgacaacg
    57481 atattttcac gccaagacaa tcgcaatctt aaccgcaaac ttgtttggtt atggagaata
    57541 ttctgccagc gtctgtttgg tacaaattga ggaatcaaca cagtagttgt atagttttgt
    57601 ttcgctgcat ttttacttac gatatctaca taacggataa ttggattttc aatcgagcga
    57661 taagaagagt ggacgattga aaaacggaca tctgggaaat gctttttaaa ttctgcttcg
    57721 atttcttttt ctttttcgac atcttcgtct agcgaaacat gcatcgcaac gacataatca
    57781 ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc gtgtcacatt cccgactaat
    57841 acaagaacgg tattcccgtc ataatcatgt agttttgcat cattttctaa acgaagctgt
    57901 tctgctactt ttttataatg atcatggatc ttataaaaaa tgaacataac gattggcata
    57961 atgatgaaga aaggccaaat gtcccctaac cgatagacaa acaaaatcgc aatgattgca
    58021 tatgagatga acgcacccgt aatattggca atcgatttgc ttaaccatct ttttccttct
    58081 tttttccatt tgacgaccat ccctgtttga gataaagcaa atggaatgaa cacgccgata
    58141 gagtataacg gaatcaagcg ttctgttgag ccatggaaaa taaacagcaa tacgatcgaa
    58201 cctagcgcta gtgtgataat cccattggaa tagcctagtc gatccccacg atcttgatac
    58261 atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa gaactggaaa agcagaaaaa
    58321 ccggtgttag cagcaactgc taaaatcagc gctgtagcaa attgaaggac gtaatacaaa
    58381 ataccatgtc caaagacagc tttaccaatt tgagacaaaa cagtgacttc tttttcagga
    58441 acgatcccat accaatagtt gataaatgtg atccccacga agaagaaacc taagatcaat
    58501 gccatcatcg tcaatgtagc agcggcattt tttgctcttg gcttcttaaa gaatggtaca
    58561 gcattactaa tcgcctccac cccagttaaa gaggaagaac cagaagaaaa tgctcgtaaa
    58621 atcaaagcga tagaaacacc cggcacagcc gctcctggaa gtgcagtagc ttgcaatggc
    58681 acagcacctg tcacgatatt atataaacca acaacgatca gtaacgagat cacaaggata
    58741 aatgtgtaca ccggaaataa taggaacgat gcagattccc tcagcccacg aagattcagc
    58801 atcatcagta gcaaaacgat tgtcacagaa atagcgactt gatgaccgta gagtgctggc
    58861 accgctgatg tgatcgcttc agcacctgct gagacagata ctgctactgt caacatgtag
    58921 tcgatcaata aagagccacc ggaaatcaag ccagcattct ttccaagatt ctcactgctg
    58981 acgacatatg ctcccccgcc atgaggataa gcatggatga tttgacgata tgatagcgtt
    59041 aatgaaatca ataaaataat aacaaaagcg gcaatgggta gcgaatacca gatggctgca
    59101 gcagacaaag cgactaaaac gacaacgatt tgttccgtac catatgcaat agatgataac
    59161 gcatcagacg acaataatgc caaagcagca aatcgggtta atttgtgctc atcattttca
    59221 gcagatttta aaggcttacc tactaacaac ctttttaagt aatccaattt ctaaactcct
    59281 ttacacacaa ttttacttca agttttatag tcgaaaattt atagtaaaca agatgttata
    59341 gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa ataattggaa aaaaacagaa
    59401 aaatatcaag cgctttttca aacgctttaa tacagcaaaa atctaacatt tttttatggg
    59461 ctttttcttt gaattctata ataaaaggat agaacttgac ttagaaaatc aagttctatc
    59521 ctttttgata tcttaacttt tggaagagag atgtaaggct gctttctacc catcattctt
    59581 actactcgaa gcatagcgca gttgtcacaa cctcttgata aacggtgttt aaaagcttgc
    59641 atctgcggta ataagcccaa ccaaattaaa aacataagaa gctgttttta tttggtcgtt
    59701 cttattactt gatgcagagc agctttttta caacctcttg gtacacggtg ttcaatcagc
    59761 tgaccctcgg tattagctca aaaaaacggc aaaatatgag gaactatttt tccatttttt
    59821 tctctaatac tcaggtcaaa acactgattt cacaacctct ttttttaatg aaaaatatgg
    59881 ctctcatgcg ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc
    59941 gttcctacga ttccaaagac aacaaacaaa ccgatcatga tccagattgc tttcgtagca
    60001 tttggccaat aaattcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc
    60061 aagaacggat tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct
    60121 ccgccagaga tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat
    60181 aatgcagcca agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac
    60241 acgctgtaga ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg
    60301 atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct
    60361 cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt
    60421 gaaagaagaa cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa
    60481 ccgttatttg cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag
    60541 tcactttctt ttgttgcgtc tagtttcaat aatttgatga cttcgcctag atcgacttct
    60601 tgttcgcctt ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag
    60661 tcattgttga tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca
    60721 tttactgttg tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata
    60781 aaatcagcgg ccaatcctaa tttttgctga atgatcggta attcattgtt gtataggtct
    60841 gccccttcat tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca
    60901 tcatggattt cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact
    60961 gcattcgtta cagttgcttt cgttgagctg agaagcgaat caaagtcgat ctggtccgct
    61021 ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga cttggtctag tagagaagtg
    61081 acagtggaac tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca
    61141 acggcatcaa tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct
    61201 gtgttcaaag cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc
    61261 tgatcaataa ttggctgtaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac
    61321 tcgaccagtt tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat
    61381 acattgctga tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt
    61441 acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc caattgactt caatgtaaca
    61501 tctacactgt ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt
    61561 tggtttgcta gatcacttgc ctgattttct aaatttttca catctggcaa gatatcttgt
    61621 acttgttgga tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct
    61681 tggatcgaag caaaatcgcc gtcgatctct gctagttgct taccggcatt ttggatctct
    61741 gggatttttt cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg
    61801 tcattcaatg caacgacttt ttcacccatc tcatctactt tagggatgta atccacgaat
    61861 tcatttgctt tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact
    61921 tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatcttta
    61981 actttattga tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc
    62041 aataaagtcg aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt
    62101 gctcctttat cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg
    62161 atttttggtt tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt
    62221 ggcaagtaaa tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt
    62281 gaatcaacga attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc
    62341 ccaatggcca cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt
    62401 ggcagctctc cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat
    62461 agcgaaggga tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc
    62521 cgtttccagt caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc
    62581 cttcttcttt tttcagctga attactttac catgtatttc atactttttt caatcggctt
    62641 ttttcaaaaa agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac
    62701 atattgagaa ataattaagg attacagcct ttactttatc atgaaataaa agggaaagat
    62761 aattttatgc caaagttttt atctattata cgtatacgca aaaaagctaa aacatttttt
    62821 gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt atttcttatt
    62881 tttcgaatga caggatgatt ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt
    62941 ggatgacatg cttaagtgaa gcagacagat tcattgaaat aaaaacatga aaggctcctt
    63001 tttggtaggc tttgattatg ccagcaaagg cttctgcatc tagatattcc tttcttggaa
    63061 caaacgattc ataaaagcct tccttttttt gccgcaaatc tagatctggg aggtcattta
    63121 atgcaacata cctgccgcct ggtttcatga tctggatgcc ggtttctcct ttgatacttc
    63181 ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa ttgcagacct ggatttgttt
    63241 tatcataagc agcaaatgct gaagctccta atttttttac cttttcctca tttttactgg
    63301 atgcactggt taagattcgg attccctttt catggagaag ctggatcaga cttgacccga
    63361 ccgcacctga tgctcctagt atcattacag tatctgtcgg ctggatttcc gtcaaatggt
    63421 tgattagatt ataagccgta attcctggcg tgaccattcc ggctgcttct tcccaactca
    63481 ttttatctgg aatttttgct acttttgctg atggcaacac gacttcttct ccgtaagtcc
    63541 cgccgactgc atggacagct actcgatcac ctacatgaac agttgtgaca tctgaaccaa
    63601 tctctgtcac aataccagcg ccatcattac ctggtacata agggaatttc aatgttcgta
    63661 tctctttcat ttctcctaaa cgcaaggcaa catcataagg attcacactg aatgctttta
    63721 tttctacacg aacatgtcca gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa
    63781 catcttgtgc ttctccgtaa ccgttaattg caaatcttcg catggtcacc atctcccttt
    63841 ttctttaagt ttacgtttat gcctttattt tgacaaaaaa aatgtctttt cgttttggcg
    63901 catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg tgttaaatta tttatctaaa
    63961 agcgtaaaga ttacgattta cgctaataaa aaataattgg aggaatttgt tgatggctaa
    64021 aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg attgaacgct atgctgcttt
    64081 acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg cgtaaacttc ctttagatgc
    64141 tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga cgccctcgcg gatacatgcg
    64201 taaattcggg atgtcccgta taaaattccg tgaattagca cataaaggtt tgattccagg
    64261 agtaaaaaaa gccagctggt aatccaagtc tggctgtaat gtaaaggagg agggcgcatg
    64321 gaccactcct tggatcatca aaaaagatac gttcgcttct tctagcagaa atgctggtac
    64381 tagaagcaga cgtatctttt tttacgcaca gctggtcctt ttttatatgt aaaggctgca
    64441 tggttacttc gctatattta tttagtaaca ggagtcagtg cttcgatacc cacactaatt
    64501 ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca aatggcagac cttttctttc
    64561 aatcttgatt tatcgatcgt tctgatctgc tccaacagga taacggagtc tctttctatt
    64621 cctgttccag tagctttgat cccaatgtga gtcggaagct taggtttagc catttttgcc
    64681 gttatggccg caacaatgat cgtcgggcta aaatggttac ctagattatt ttgaataacc
    64741 aaaactgggc gtgttccgcc ttgctccgat cccacgactg gagaaagatc agcaaaataa
    64801 atatctcctc tttttaccat aggatcctcc ttattcttga tattctcttg gtatccgctg
    64861 tcctaatccg caagccactt catagtggat cgtacctaat tgatctgcta tatcctgcat
    64921 agtgatttct ttgtctgcat ttttgccgat aagcgtcact tttgttccga caggaaattc
    64981 ttgaggcaat cggatcatca gttgatccat acatacacgc ccaatcgttt cacagtagtt
    65041 cccttctacc aatagagaaa aaccctgcat ctttcgtggc cagccatctg cataaccgat
    65101 cggtatcgtt ccgatccact ctgcttcagg agtgatatat gtttctccat agccgattcc
    65161 ttcacctgct gggagttttt tgacttgaat caattcagaa accagctcaa gtgctggctg
    65221 caatggataa acttccggta gtgcgtgccc agagggatta agtccgtaca ttgctactcc
    65281 ataacgaatc atattgccga ttgtttcatc atgccataaa gccgttgcac tattgctgac
    65341 atgtacatat cgaggaagtt cgggaagttt tttcagcact tctataaagc gttctttttg
    65401 cagattccaa tatgtatcat ctgcctgatc tgctgtagaa aaatgggtaa ataaaccttc
    65461 ccattctaat acacgacttt ctttgatgaa tgcagctgcc tcttttactt cttcaggtgt
    65521 acaaaatccg atacgaccca tcccagtgtc tgcttttaga tgaatcgaaa gaggcgtctc
    65581 tagcttactt ttctctaaaa cagcagctgc tgcttgcaac cattcccgtg tacctgcagt
    65641 tactgataaa tcatgagcaa cagctacagt cagtgcgtcg taaggaacca tattcaagat
    65701 aaggatgggc tgagtaaatc cagcttctct caactcgact ccttcatcta aatttgaaac
    65761 acaaaagccc gaagctccac ctgcaactgc tgcctcagct gtttcaatag ctccatgacc
    65821 gtatccgttc gcttttacta ctgcaaacaa ttcttttcct tgcggaagtc ttgcaacttc
    65881 atttgctaca ttttcagtta tagctttttt atgtatcact gctttcgttg ggcggtgcca
    65941 agcgacaacc atcgtttttc cttctttcta gtcttccaaa ataatttgtg caaaagctgt
    66001 ttcatttgta tgagtaatac tgacccatac attgccttca tgaggtgaac gcgtcacaac
    66061 aggctgacca ttttcatttt ttaaaatctc gacatcttga aaggtaactt ttccgatccc
    66121 agttccccaa gctttagaga aagcctcttt gcaagcgtaa cgcccgccta aaaattctac
    66181 ttggcgatga aaaggcaagg actggaatag tttcatttca tcagaggtca agattcgtgc
    66241 aatgaatttt ggtttttctt cgatcagccg agtgatccta ggaagttcga ctgcatcgat
    66301 cccaattcct tttatcatat gcttttctct tttcaataaa tttagaacac tctcattcta
    66361 ccaaattata accattttgc cacaaataag tccacaaata acaaaaatga aagtttctct
    66421 taaaaaagag aaactttcat tttatgttta atcgttattt gaacggatta caaatccgcg
    66481 ttttttatca ttgtgacgtt taccgccatc tttttggctg ttattatttt tgctataacc
    66541 gccttttgat ttgtttccac gataattacc gccgtcacga cgattacggt tgtttccgcc
    66601 accacgtttg ccatttttat tgtatccttt tttctgcatt ggcaatgggc gttctggtgt
    66661 gatcttcact ggcactgcat cagctggatc ttttgctgtc gtttttagca aaagtgcaac
    66721 taaatcttgt gcagaatatt cttctaataa tttatctgct gtttgcaggt atttatctag
    66781 accattttct gcaagttttg tttcgatttg ttcaactgca gcacctaatt gacctttgaa
    66841 tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt tttttcgtca agttttcaat
    66901 cacatgcaag tagtccattt catttggtgt aacgaaagtc acagacatac cgccttttcc
    66961 ggcacggcct gtacgtccga tacggtgaac gtagctttct ggatcttgag ggatatcata
    67021 gttgtacaca tgagtgacac ctgaaatatc taatccacgt gcagcaacgt ctgtcgctac
    67081 aaggatatct aaatgaccgc tcttaaatga acgcaatacg ctcattcgtt tttgttgaga
    67141 caaatcgcca tggattcctt ctgcacggta accgcgtgct tctaatccgc gtgccaattc
    67201 gtctacacgg cgttttgtac gtccgaagac gattgttaat tcaggtgttt gaacatcaaa
    67261 caagcgagtc atgatatcga atttttcgta ttcttttgcg cggacatagt attgatcgat
    67321 caagtcagct gtcatttctt tcgctttgat cttcacatga tgtggatttt tcatgaattt
    67381 aacgccaata ttcttgatag ctggcggcat cgttgctgag aaaagtaatg tttgtctttg
    67441 atcaggaact tgtgaaatga ttttttcgat atcttccaag aagcccatat tcaacatttc
    67501 atctgcttca tccaaaacta atgtttgaac ggttccaagt ttcaatgtgt ggcggttaat
    67561 gtggtcaagc atacgtcctg gtgtacccac aacgatatgt ggacgatctt tcaagccacg
    67621 gatctgacgg ccaatatctg ctccgccgta aacggcttgt acacgtactt ttttgtcgcg
    67681 ccctaaacga tataattctt cttgtgtttg gatagctaat tcacgtgttg gtgcgataac
    67741 taaaccttgt aattcgtgac gatctggatc gattttttct aacattggta atccaaatgc
    67801 agctgttttc cctgtacctg tctgtgcttg tccaataacg tcttttccag ctaatgctag
    67861 tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa ccagcgcgtt cgactgattt
    67921 caacaattct ggtgataatt ctagttcttt aaatttcaaa tgaatcctcc tatatggttg
    67981 ttaactgagg atgtagcttt tttgctaccc cgtcgatttg ttcggtcaaa gtgtattgtt
    68041 tctttttgac agtggccacg cacgttttta tccattctta gcccctgtgg caccaccgaa
    68101 aaatcacttt gcgaagttta ggcattagaa aagccaaacg ggtcgcgttc agctttatca
    68161 ttattatgac atctggcttc aacaagttgt ttctatggca aaaccagtcc aatttctctt
    68221 catctttcgg caataggtta ttctaacata gtttttcgtt ttctgcaagt ttacagattt
    68281 ttatttcatt tgctgaagtt gttctaccac ttcaaataag cccatgctgt tgctgccttt
    68341 tagcataata ctatctgttg cttcaagatt ttgtttgata gcatcaatca atttattctt
    68401 ttctttttca aaatagaaca caggtagttg ggggtatttt tggttcaatt cttcttttag
    68461 tgcatgcatt tgttccccat aaaggaaaat catggcgaat ttctcaggat cgatatgttc
    68521 actcatttgc gcatgcatat ttaaagactc tttccctaat tccagcatat ctgctaacac
    68581 agcgattctg cgtccttttg ttggcagatt tttaaaagta tctaatacca gtcccatggc
    68641 tgttggattt gcattgtaga catcgctcaa aatagctgca ccatttcctg ccgttagcca
    68701 ttcggttctg ttttgagtga tagaaacatg ggcgagccct tggcgtatct caggtatgga
    68761 cagtccaaag aagcgcccga tcgtatacgc aatcaaagca ttcgttacat tataacttcc
    68821 aggcagcggg atcgtaaatg tttctccttc tactacaaaa tcggtttgtg cttttccttt
    68881 tgctgtcact tccgcatgaa catcgccatt tccaataccg aatgttgtga ctgtttgtac
    68941 taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg ggcacaacga gtaatccgtc
    69001 tgatacaagt ccgtcagtga tttccatttt ggctttagcg attccttctc gcgacccaag
    69061 attttctatg tgtgcttcac cgatcattgt gattgctgca gcttttggtt gagccataag
    69121 tgaaagttcc gtgatttcat tggcgtgatc catgcccatt tccaaaacga tcttttctgt
    69181 atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata ttattgttgt aatttccttg
    69241 cgtcttatat gttttgtatt tttcagacaa gacagacgcg gttagatctt tcgtggtggt
    69301 tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct actttttcca agtaataggt
    69361 tgccaattgc tgcatggctt tcaaaggatc tgctacagga ataactggaa aattttttgg
    69421 agcttgttca acaggcaagc tccaaaatga agcagatgct ccgtttgtga tcgcttgctg
    69481 gataaattca tgtccatctc gcgtaccagc aagaggaacg aataaacttc cttcggttat
    69541 cttccggctg tcaaattcga cattggtaat ctttgtcagt gcagagcatt cgctgcccac
    69601 ggcttgtgca atttcttgta ttgttagatt cattgagatc tctcctaaaa aaggttgtga
    69661 aaaaagcgtt tggcctaaac ggcgggttcg catgactctc acttccaagt aaatttctct
    69721 attcgtattt agaagctaga atgaacgcgg ttacgcaatc actccagcct cttctttatt
    69781 tctctttatt catttttttc aaaaaaagat tgacgctgat ggtatcgatt cattcctaac
    69841 tggatcagtt cttcaatcaa atcaccgtat ttcaagccca tattttccca taagagtggg
    69901 tacatactga actccgtaaa tcctggcata gagtttaatt cattcaggaa taattcattt
    69961 ttatttgtca aaaagaaatc gcaccggctc aatccgcttc cacctaacat cgtgtaagct
    70021 aacttcgcgt actcttgcgc tttttgataa acttcttctg gcacttcggc tggaatctgc
    70081 atttcgattt tattattgat atattttgct tcataatcat agaatgctac gtcttttacg
    70141 acttcaccag gcaaagtcgt ccgaacgtct tcatttccta atacagcaac ttcgatttcg
    70201 cgcgcttcaa ttccttgttc aacgattgct cgagaatcat actgataggc tgttgctaaa
    70261 gcattttgca gctcttctcg gttttctgct tttgtaatgc cgacactaga acccatattc
    70321 gccggtttga caaacatcgg ataaagcaaa gaaccttcac attgatcaaa tactttttta
    70381 ggattttctt tccattgatt cttaagtact ggtacataag gaacttgcgg cacaccagca
    70441 gcttgtaaaa tatacttggt catgatttta tccatggcac atgcactggt caatacgcct
    70501 gcgccgacat aaggcatatt caatgtctct aagaagcctt ggatcgttcc atcttccccg
    70561 tttggcccat gtaaaactgg aaaaacgatg gctccttctt ctttgatttc gcccggattg
    70621 atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg ggtcccatga aagatgcaag
    70681 acatctttgc tggcaggttt ttctgttagt aatggacctt tgacccattg tccttctttt
    70741 gtaataaata cgagttgaac ttggtaataa ttataataaa tggcatttaa aactgaaaat
    70801 gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc catatagtaa agtaatcttc
    70861 aaaagattgt cctcctgtat tctaaatcgt tcagtgattt actctcatat tttagcacaa
    70921 aacaaaaata aacgaataac ttatttataa aaaaacgagc cagcttctaa aagaacgagc
    70981 gtcatttatt ttttgtcagc tagattattt gtcccgtttt ttataaaaag ttatgataaa
    71041 gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag ccgcttggca gaaatcattc
    71101 gagtgttcat caattacaat gtagtgccaa actttgtaca gcaaaaaaat cccgaacagg
    71161 taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa gataggacag atgctttctg
    71221 ttcgagaaga tttgctttct tctgccttca ctcaaacatt caaaacatta caagatagtg
    71281 tacctagcga cacattttca acggtcaaaa agacgatcga aacagaactt tctctttcat
    71341 tatcagatat attcgatgat ttttcaaaat cgccttttgc ctctgcttct atggggcagg
    71401 ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt taagatccaa caccctaaca
    71461 tcgccgaaga gatccgactt gatcttcaac tatttgaacg agcgatccct ttgatcaaat
    71521 acattcctga aactagcgta gttgatctaa aaggggtact ccaagaagta aaacgttcct
    71581 tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg cgaacaattc tatcaaaaga
    71641 acaatggctg gaaagagatc cgcagcccca agatttatga cgctttttgt tcgaaaaaag
    71701 tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca tttaatgaac gccgaaaaca
    71761 aaactgaaac ttttatcact ggaattcaaa acaagcagct gaaacaagaa gttgcaaagc
    71821 ttttagttga gaactttatg aaacaggtgt ttgatgatgg ttttttccat gctgatcctc
    71881 atccagggaa tctgctattc catgtactaa caaaagaaga acagacacaa gctagccgaa
    71941 aaactgaaac agtccatgaa aaagagttcg gttcctttgc ttttcgagcc tccacttctg
    72001 cagaagaccc tgttgcgcca tataccatca actacatcga ttttggtatg atggggcatc
    72061 tttctgcagg acttaggcaa aaactcacac aagccgtttt agcgctgtat acgaaagatg
    72121 cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca agaaggctcg tttgatgaaa
    72181 gtcggttcca ccaagaactg acaagttttc ttgaacagta ttatgattct cctattgatg
    72241 aaatcaactt gcaagaagta ttcacacatg tcgtgacgat ttgtcatcaa aataatctgc
    72301 agttcgatcg tgatatcacg cttttgctga aagcatttgg gacattagaa ggggtgatcc
    72361 gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc gccttttgct caacattatt
    72421 ttctcactca tctggatgta gaagatacat tgaaacaaag cggattagac ttattggaag
    72481 ggatgaaggc tgcgcctaag atccctcaac agctccacca tctattagaa atgtggactt
    72541 cgggacaagg aaaagtcaat ttggaattga aaaagcaaga caaattattg agcaggatcg
    72601 aaagtatgat caatcggctt gttttcggta tgattttagc tgcattgatc gtagggtcct
    72661 ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt cgtttcctta ttagggatat
    72721 ttacgtatgc tattgccgca ttcgtcatta tttttttggc aatcgatgca ttgatccaaa
    72781 tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga aagaatcagc ttcaagaaat
    72841 aagaaggggt tttacataaa ttgtttctca aatttttgtg aaaattcggc ttatttttcg
    72901 aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa aaagctgttc tgcacccagt
    72961 aataagaaca atcaaaaagg gaacatggaa cacatttatc ctgtttccct gttccctttt
    73021 tgatgtattc gagcataaat aaaatacctt cgttacctac tatcttctgt acttggtaaa
    73081 ccttagtcaa ttgccttttt taacgcttga cgaaaagaca tatgatcttt ctccgtaaag
    73141 ggttttggac cctttgtcct tatgccagct tttcttaact ttccactatg ataacgttgt
    73201 tctagaagcg tgccgatatt cgcttcgtca aattcttttc cagagtgatg gaacactcgc
    73261 acacccttcg gcaatacaag tgaagctaaa ccataatctt gcgtgatcaa aaagtcattc
    73321 ttcttaacca taccgacgat tttataatcc gcactatcag ctccgcggtc tacgtagacg
    73381 atttggacat gattagcgta ttctttggtc gtataatggt ccacactggt cacgataacc
    73441 acgtcaagac cataggattc tcctaatttt atgacatctt cttttactgg agaaccatct
    73501 ccatcaatga atagcctcat tctttttcgc cttcttccat aaatgataaa cagttactaa
    73561 tccgctcagt aaaagaccaa cattgatcca ttttgccaag ataagcagtt gtacactttc
    73621 tatcggcact gattcatggt tcagcgtgat caataataga tggattggct ctcccatttc
    73681 ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg tggatcacac tccctttatt
    73741 ttgagtagta tacttgacaa cgatccggcg atccattgga atagctgtat cagtttgata
    73801 atattcctta tttagccagc tgcctgttac tacgtgccag aaactaagca gaaagacacc
    73861 tatcgcaaaa ccatttggaa tcaagaggac caataaagaa gaactcagtc caagcaattg
    73921 catagccaat ggagaccatg ttaaacgttt caaaatccat ggtccgacta tttttgctgc
    73981 aatcaagcct gctgcataag gcaaatacat aaaaatcgaa agtttttcta atccgtacag
    74041 tatgccaaca taaaaactgc taaataaaaa aaggaaattc cctagcattc catttagaaa
    74101 agtaaacaga tttagccagc ttggcaactt ccattccttc ttctggtgtc ctaaaacaaa
    74161 cacgaacaag atgaacagca gcaagaaacc ataaatagca agatcaaaaa gcctttcatc
    74221 gaataacaat cgggcatttc tcaaaagaaa caataagcca aagaatatca aaaataaaac
    74281 aagctctcgc gtagctacca gatttctttt gatgtctttg aaatccagtt cgtaacgcgg
    74341 ataatgacta accgtatggt aggccatgac gtaaaaaagt gtataaatag taaagcttgc
    74401 tgcaatctga gtcggtgcag gcaactcaat cgcttgatac aggagaaata gccagatagc
    74461 tgcaaataag tactttttcc catccatatt gataaaacct tgcgtctttt cgtgaaaatt
    74521 gaccgtggta ttagcaggta caagccatgc tgcactcagc cctagcaaaa cacttgaaaa
    74581 ataaaataaa ggaaaataaa atacgcctaa tattccaagc aatgaaccag caccaccaat
    74641 cagcagagcg atcatcagca acgtgtaact atctagacca aatcgtagac tgttgactaa
    74701 aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat ggcaaaatcg tcagccaatt
    74761 atgcgagtct gttagtccca aaaataaaag ataaggaata aaaataaccg tgttacttaa
    74821 gataaagggc gttgtttgtg taaaattacg cccaaatcgt ttgagttttt cgaacatgac
    74881 aatggctcct taaacagtct tataactaat ctgctcttcg attttagcat taaaggggaa
    74941 ataaaccaat tttacagaat atattcttgt aaaaaaaaat acagaaagaa gcataagctt
    75001 ctttctgcat ttttacggta cttgattact caagcagaca agcccttctc cacaaaagtc
    75061 ttgcccgaca cccctgcatc cggcaggtga taaagataga aatcaattat cctgtgcctc
    75121 caaaatcaat taggagctct gtaaaccaga gtccttctcg acccgtcgca taaatttact
    75181 ttaacacagt tcctatgttt atgcgagagc ttttttcatt ttataatcga ccatttgaaa
    75241 tccttgaccg atatctgaaa taagttcttc tgtaatctca aatccttcat gcttataaac
    75301 ctcgattgct tgatgattcc cctgattcac tcgcaaaaag atctctcttt cctttgtttg
    75361 cgcagcaagt tcttcatacc agcgaaaaac tgaagaagtc aatccttgac cacgtacttt
    75421 atttagcaaa tataatttac ttaaataaat ttttccattt atctcttcat aagcagtgta
    75481 accgacagtc ttttcttcgc tttttaataa aaagtaggac acaccttctg tcagttcttt
    75541 ttgaatctgt ttttttgact gataagtttt cagcatgtaa tccacttgtt tttgaccaat
    75601 gatcggcaca aaaacttccg gccatacttc ttgtatgact gccatcagct catccagttc
    75661 attttctgtt acttttattt tttccataca cccacgtcct tttttacatt ataaatgacc
    75721 ttgcgcttga tggaataaaa agagcatgat catgctatac acacccaccg accacggttt
    75781 caagaaaatc acaatctgca taaattccct aaaagataat ttcgtcaaac cagctactag
    75841 acagatcaag tcgtctggcg caaagggaag cagcaaggta acaactagta cttttttaat
    75901 atctttcgtt tttcttgtca gcaattcatc aaatttctga tacttttttg gcgataaaat
    75961 aagctggaca aaggctacgc catagtaacg gacaagcaaa aatccgattg cttccccaat
    76021 cactaggcca atacaactat ataaaagccc aaatccattg ccaaacatca gcatgcccgc
    76081 tactgaggag atccctcccg gcaaaatagg aatgatcact tggatcactt gcaacagaac
    76141 aaatatcatc actgcatact tgccaaattg ctggataaac gcttgcaaag acgtgctcga
    76201 tcgaaaaatc ccatgatgat aagcataaag aatcaacaca aagaaaaaaa acagaccaat
    76261 aatgggcatg atctgaagac atttttgtaa aactgatcct tttttagaca tctatattcc
    76321 ccacatcttt tttcatttat taaaactgcc tcctt
//
LOCUS       DACDIP010000008        73285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDIP010000008 DACDIP010000000
VERSION     DACDIP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.008"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..1227
                     /locus_tag="IV257_000618"
     CDS             169..1227
                     /locus_tag="IV257_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8813769.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1246..2265
                     /locus_tag="IV257_000619"
     CDS             1246..2265
                     /locus_tag="IV257_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8813770.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2367..3083)
                     /locus_tag="IV257_000620"
     CDS             complement(2367..3083)
                     /locus_tag="IV257_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8813771.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3052..3838)
                     /locus_tag="IV257_000621"
                     /pseudo
     CDS             complement(3052..3838)
                     /locus_tag="IV257_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(3829..4578)
                     /locus_tag="IV257_000622"
     CDS             complement(3829..4578)
                     /locus_tag="IV257_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8813772.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4876..5400
                     /locus_tag="IV257_000623"
     CDS             4876..5400
                     /locus_tag="IV257_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813773.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5416..6915
                     /locus_tag="IV257_000624"
     CDS             5416..6915
                     /locus_tag="IV257_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8813774.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7003..7605)
                     /locus_tag="IV257_000625"
     CDS             complement(7003..7605)
                     /locus_tag="IV257_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8813775.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7709..8665
                     /locus_tag="IV257_000626"
     CDS             7709..8665
                     /locus_tag="IV257_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8813776.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8970..9926)
                     /locus_tag="IV257_000627"
     CDS             complement(8970..9926)
                     /locus_tag="IV257_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8813777.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10100..10660
                     /locus_tag="IV257_000628"
     CDS             10100..10660
                     /locus_tag="IV257_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813778.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10653..11477
                     /locus_tag="IV257_000629"
     CDS             10653..11477
                     /locus_tag="IV257_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8813779.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11487..12104
                     /locus_tag="IV257_000630"
     CDS             11487..12104
                     /locus_tag="IV257_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8813780.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12122..12805
                     /locus_tag="IV257_000631"
     CDS             12122..12805
                     /locus_tag="IV257_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8813781.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13488..14771
                     /locus_tag="IV257_000632"
     CDS             13488..14771
                     /locus_tag="IV257_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8813782.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14787..15674
                     /locus_tag="IV257_000633"
     CDS             14787..15674
                     /locus_tag="IV257_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8813783.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15691..16527
                     /locus_tag="IV257_000634"
     CDS             15691..16527
                     /locus_tag="IV257_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8813784.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16530..17153
                     /locus_tag="IV257_000635"
     CDS             16530..17153
                     /locus_tag="IV257_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813785.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17150..18442
                     /locus_tag="IV257_000636"
     CDS             17150..18442
                     /locus_tag="IV257_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8813786.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18801..18898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19014..19595
                     /locus_tag="IV257_000637"
     CDS             19014..19595
                     /locus_tag="IV257_000637"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8813787.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19598..20905
                     /locus_tag="IV257_000638"
     CDS             19598..20905
                     /locus_tag="IV257_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8813788.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21321..21809
                     /gene="purE"
                     /locus_tag="IV257_000639"
     CDS             21321..21809
                     /gene="purE"
                     /locus_tag="IV257_000639"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8813789.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21793..22926
                     /gene="purK"
                     /locus_tag="IV257_000640"
     CDS             21793..22926
                     /gene="purK"
                     /locus_tag="IV257_000640"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8813790.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22939..24234
                     /gene="purB"
                     /locus_tag="IV257_000641"
     CDS             22939..24234
                     /gene="purB"
                     /locus_tag="IV257_000641"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8813791.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24414..24791)
                     /locus_tag="IV257_000642"
     CDS             complement(24414..24791)
                     /locus_tag="IV257_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813792.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            25047..25775
                     /locus_tag="IV257_000643"
     CDS             25047..25775
                     /locus_tag="IV257_000643"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8813793.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25775..26029
                     /gene="purS"
                     /locus_tag="IV257_000644"
     CDS             25775..26029
                     /gene="purS"
                     /locus_tag="IV257_000644"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8813794.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            26031..26702
                     /gene="purQ"
                     /locus_tag="IV257_000645"
     CDS             26031..26702
                     /gene="purQ"
                     /locus_tag="IV257_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8813795.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26703..28925
                     /gene="purL"
                     /locus_tag="IV257_000646"
     CDS             26703..28925
                     /gene="purL"
                     /locus_tag="IV257_000646"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8813796.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28910..30349
                     /locus_tag="IV257_000647"
     CDS             28910..30349
                     /locus_tag="IV257_000647"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8813797.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30381..31424
                     /locus_tag="IV257_000648"
     CDS             30381..31424
                     /locus_tag="IV257_000648"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8813798.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            31421..31999
                     /gene="purN"
                     /locus_tag="IV257_000649"
     CDS             31421..31999
                     /gene="purN"
                     /locus_tag="IV257_000649"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8813799.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31996..33531
                     /gene="purH"
                     /locus_tag="IV257_000650"
     CDS             31996..33531
                     /gene="purH"
                     /locus_tag="IV257_000650"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8813800.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33547..34821
                     /gene="purD"
                     /locus_tag="IV257_000651"
     CDS             33547..34821
                     /gene="purD"
                     /locus_tag="IV257_000651"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8813801.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34943..35857
                     /locus_tag="IV257_000652"
     CDS             34943..35857
                     /locus_tag="IV257_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8813802.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35990..37555)
                     /locus_tag="IV257_000653"
     CDS             complement(35990..37555)
                     /locus_tag="IV257_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8813803.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37548..38999)
                     /locus_tag="IV257_000654"
     CDS             complement(37548..38999)
                     /locus_tag="IV257_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8813804.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(39013..40029)
                     /locus_tag="IV257_000655"
     CDS             complement(39013..40029)
                     /locus_tag="IV257_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8813805.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            40249..41337
                     /locus_tag="IV257_000656"
     CDS             40249..41337
                     /locus_tag="IV257_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8813806.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41391..42917
                     /locus_tag="IV257_000657"
     CDS             41391..42917
                     /locus_tag="IV257_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8813807.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42919..44124
                     /locus_tag="IV257_000658"
     CDS             42919..44124
                     /locus_tag="IV257_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8813808.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44381..45358
                     /gene="guaC"
                     /locus_tag="IV257_000659"
     CDS             44381..45358
                     /gene="guaC"
                     /locus_tag="IV257_000659"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8813809.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45622..47046
                     /locus_tag="IV257_000660"
     CDS             45622..47046
                     /locus_tag="IV257_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8813810.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47507..48616
                     /gene="pdhA"
                     /locus_tag="IV257_000661"
     CDS             47507..48616
                     /gene="pdhA"
                     /locus_tag="IV257_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8813811.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48619..49596
                     /locus_tag="IV257_000662"
     CDS             48619..49596
                     /locus_tag="IV257_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8813812.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49621..51264
                     /locus_tag="IV257_000663"
     CDS             49621..51264
                     /locus_tag="IV257_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8813813.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            51272..52678
                     /gene="lpdA"
                     /locus_tag="IV257_000664"
     CDS             51272..52678
                     /gene="lpdA"
                     /locus_tag="IV257_000664"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8813814.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52840..53115
                     /locus_tag="IV257_000665"
     CDS             52840..53115
                     /locus_tag="IV257_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8813815.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            53121..53888
                     /locus_tag="IV257_000666"
     CDS             53121..53888
                     /locus_tag="IV257_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8813816.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            54056..55885
                     /gene="typA"
                     /locus_tag="IV257_000667"
     CDS             54056..55885
                     /gene="typA"
                     /locus_tag="IV257_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8813817.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            56146..57918
                     /gene="recQ"
                     /locus_tag="IV257_000668"
     CDS             56146..57918
                     /gene="recQ"
                     /locus_tag="IV257_000668"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8813818.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            58032..58334
                     /locus_tag="IV257_000669"
     CDS             58032..58334
                     /locus_tag="IV257_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8813819.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            58337..59500
                     /locus_tag="IV257_000670"
     CDS             58337..59500
                     /locus_tag="IV257_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8813820.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59526..62954
                     /locus_tag="IV257_000671"
     CDS             59526..62954
                     /locus_tag="IV257_000671"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8813821.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            62975..64099
                     /locus_tag="IV257_000672"
     CDS             62975..64099
                     /locus_tag="IV257_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813822.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            64089..64526
                     /locus_tag="IV257_000673"
     CDS             64089..64526
                     /locus_tag="IV257_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8813823.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64510..64848
                     /locus_tag="IV257_000674"
     CDS             64510..64848
                     /locus_tag="IV257_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8813824.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            64967..65527
                     /gene="rsmD"
                     /locus_tag="IV257_000675"
     CDS             64967..65527
                     /gene="rsmD"
                     /locus_tag="IV257_000675"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8813825.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65524..66015
                     /gene="coaD"
                     /locus_tag="IV257_000676"
     CDS             65524..66015
                     /gene="coaD"
                     /locus_tag="IV257_000676"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8813826.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            66005..67048
                     /locus_tag="IV257_000677"
     CDS             66005..67048
                     /locus_tag="IV257_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8813827.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            67207..67935
                     /locus_tag="IV257_000678"
     CDS             67207..67935
                     /locus_tag="IV257_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8813828.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            68051..68296
                     /locus_tag="IV257_000679"
     CDS             68051..68296
                     /locus_tag="IV257_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8813829.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            68293..68511
                     /locus_tag="IV257_000680"
     CDS             68293..68511
                     /locus_tag="IV257_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8813830.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68647..69186
                     /locus_tag="IV257_000681"
     CDS             68647..69186
                     /locus_tag="IV257_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8813831.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            69179..71452
                     /locus_tag="IV257_000682"
     CDS             69179..71452
                     /locus_tag="IV257_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8813832.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71490..72536
                     /gene="holA"
                     /locus_tag="IV257_000683"
     CDS             71490..72536
                     /gene="holA"
                     /locus_tag="IV257_000683"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8813833.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72625..72879)
                     /locus_tag="IV257_000684"
     CDS             complement(72625..72879)
                     /locus_tag="IV257_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8813834.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
BASE COUNT        23755 a        12569 c        16046 g        20915 t
ORIGIN      
        1 ttttttgtgt tctagtaaaa atcactttac ataagtttca aaagtttctt taatttccta
       61 ttgttcaata agtaacaatg taaagataaa attaaaaatg ttaatacgta ttagcaaaat
      121 aaaagctttt tttattgtga ataaatgaac ataattagga ggagaaagat gaacttattt
      181 gacatttttc gatcgacttt gacagatatg tcgattgtca gtgcaatcac ttcaacagtt
      241 tttattatat tattagggtt tgtttgtaga aaaaagggga ttttttctga atctgttgga
      301 aaaattctat cgaaagtcgt cttaacagtg gctttgccag cactggcatt tcgagctttc
      361 atgcaagata tcaatacaga attactgatg caaggaatca atgtcttgat ttggggatta
      421 gttatttata ttattcttat tctagtttca aaaccttttt tcttaagcta taaaggggat
      481 aagcaaacaa cgttgcgtgt cctgacgatc tttggttcta cgaccttttt cggaacgcct
      541 atcgtcagtg cgatttatgg tcctgtcggc gtgatgtatt cctcaatttt caatattggc
      601 taccgaatct ttttgtattc ttatggatac ataaaaatga gtggcttgaa gatggaagct
      661 aaaaatatca aagcgatgtt cgtcaatcca atcattatcg ctacttttgc cggccttttc
      721 atttggctgt tccaagggta tctgccacaa gtgtcagtta caggtgcaga aggagagata
      781 agccaagtag cgtttttgcg aattgaccag actgcaccgt ggctatttca gccgatggat
      841 tatttagctg gtttaagttc accattggcg tggctagcaa tcggcgcaac attaggtgaa
      901 gtaagcttca aaaaagctgc atctgaaaaa acttcatggt attacagtct agtcaaagtg
      961 gtatttgtac ctgcattgaa tatcgtgatt ctagcagttt tgacggcaac gaatattcta
     1021 ccagtcaatt ttgaagcact ggcaacaatc gttatcatga tggctacacc agctgcaaca
     1081 gtggcagcag cctatgctat cagttttgat agggaggcac ttttagcctc caatgcatcc
     1141 ttactgtcaa ctatttttgc tgtggtactg acacctgtat ggatcgttgt attacaaatg
     1201 attcatggca taggtttatt ttcataagaa atggaggaag aaagtatgag aatcgcatgt
     1261 tacggtgttc gtccgaacga gatagatttt tttgaaaaat tgaatattta ccattatgat
     1321 ctcagtcttt atgaagaact gttgacacat gacaatatcg tgtcagcaaa ggaccatgat
     1381 gctgtattgt tgaggggaaa ttgtatagca gatgagacca atcttgcaaa aatgcaggaa
     1441 tatgggattc gttatgtttt tacacggact gtgggagtca atcatatcga tctacaagcg
     1501 gcagctgatt ttggtatgac agtagcccgt gtaccatcgt attcgccaaa tgcgatagca
     1561 gagttatctt tgacttttgc catgatgctt ttgagaaata ctgcgtatac gacgatccga
     1621 acatctttca aagattttcg agtggatgag caaatgttta gtcgtgaaat cagaaattgc
     1681 acagtaggga tcataggaac aggcaggata gggttgacgg aagctgtttt gtttaaggga
     1741 ctaggagcca aagtgatcgg ctacgatatt catcagacag aagctgctaa aaaagtattg
     1801 acgttccact cgcttgaaga gttattagcc aaaagcgata ttgtcagtat tcatgtacct
     1861 catatcccag gtgaaaacga tcaaatgatc aatgaaggat ttttagctca aatgaaaaaa
     1921 ggtagcatct tgattaatac agcccgtgga gagcttcaag ataatgaagc tattttaaga
     1981 gctttgaaaa gcaatcattt agctggtttt gcaaccgatg tttttgcaaa tgaaacagag
     2041 gtttttttcc gatccttcaa gccatgggag acgattccag accctgctat ccagcaatta
     2101 gtagaaatgt accctagagt tcttgtaacc ccgcatgtag gttcaaatac agatgaggca
     2161 ttatcaaata tgatttcgac aagtttcgag aactttcgtg aaataataga aactggaaaa
     2221 acgaaaaatg aggtatctct tcctaaagcc cgtcaattaa gataagaaaa catggatatt
     2281 cgtcaataaa atgtttaaaa atagtccaag taaacagttg ttacttctgt atacttggac
     2341 tattttgtat gggataattc gatgactcaa cagtaatctg tgagcttttt ccgcccattc
     2401 tttgtcaatt cataacaggt tagcggccgg ccgatttcca aatatttgat tctttcgttc
     2461 aatatatctt gttctactaa aaaattcaaa tatttcttga cggatatacg tgacagtgag
     2521 cttttttgag ccagttcttt tgttgaaaaa atagtatctt cttttagaat aaatgcactt
     2581 atttttttta aagtcagttt tgacagacct ttaggcaaat cgatgaatga tggtatttcg
     2641 tgaatgggag tggtgtggtt gaatacttga tcgatcaatt tctgatcggc tttttcagtc
     2701 gtttggattg tctgagagaa agcaagatac ttttcgatcg ccagctgaaa acgttgcaaa
     2761 gtgaacggtt tgatcaaata atctaaggca ccgtagcgga tcacattctt tatggtttcg
     2821 atatcatctg cagcagtgat caaaatcact gggatatcat ttccttgctg tctaagccaa
     2881 gataaaaaat ggataccact ttcgccaggc atgtatacat caagcagcag cagatctgcg
     2941 tttttttgtc tgaaatattt ttttccttct tctacatttc tgaaactttc gattacgttc
     3001 atattaggga tgctttccac aaaagtacga ttcaattctg caaccattgg atcatcttcg
     3061 attatcagga catttgtcat cattttgatc ttcctttttt ggtaattcta gatagacgat
     3121 tgtaccgcca tttgtttgtt tggaaaattc taaaaatcca ccgtggcgtt tgacgattga
     3181 atggatgaga ttcaatccaa acccacgatt gattcccttc gtcgaaaaac ctagcgtaaa
     3241 tattttttct gagatttctt ctgatatccc gacaccgtca tcgcagacgg tcacgatgaa
     3301 tacttcagct tctgtgtcat agtttaaatg aatccggacc tgtccgtttc tttcatgttt
     3361 ttgtagcacg gcttcttttg cattatcaag taagttccct aacacttgca aaagtgaatg
     3421 gatggagtca tccatctcca ttttagggat aaaagaatcg ctttctaaca gcatttcaac
     3481 agattgttct ttggcttcgt ttattttgcc taataaaaaa ccggcaattg caggtacttt
     3541 gatcagactt gagacttggc caatctcttt tttataattt tgatttagct gctggataaa
     3601 atgggtgact tcttcatact tcttcaaatc aatcaaacca gaaatgacat gaagtttatt
     3661 catgaattca tgggtttgag ctctcatgga gtttatatat tgtttcgtcc ccccaagctc
     3721 ctccattaat ttcattactt ctgatcgatt tcgtaaagtt gcgactgctc ctaaaaaaac
     3781 ggtcattgct aaaaagaggt gtaacgctag aaatagtttc tatcccattt aaatacatgg
     3841 attgagtccg aacggtcgtt tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat
     3901 tatcactgat ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt
     3961 ttttgcaggt gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc
     4021 atctacttca ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc
     4081 aagtccaagc agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac
     4141 gctaagtcca ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc
     4201 aatcgtgttg tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat
     4261 agggacgaaa tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg
     4321 tcctccaatc acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg
     4381 gtctgcgtga gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga
     4441 tgcaatcatg atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag
     4501 ctttttttgg atgagtttat cattggcaac gattttagca atgctagaga ttttatctga
     4561 cacactagat cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa
     4621 agaaacgaat gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt
     4681 tttcttaaaa aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat
     4741 gaaaacaaaa atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac
     4801 tgagtcggta aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg
     4861 gaaggaggcg tgtatatgat agggggaatc gcacttgaga caatttattg gtatacattg
     4921 ctgatttgtg caggattggc agtcctattg attatttttg gagatgtatt cgattttgat
     4981 ggcccgcttg atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt
     5041 ctaggtgagc gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata
     5101 tcgatagtgt tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa
     5161 tcaacgatgt cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg
     5221 atccctgtac gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt
     5281 ccagcttgtt tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc
     5341 attgagatca aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa
     5401 aggagcgtgt gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg
     5461 attttactga tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa
     5521 gcgttgatca tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat
     5581 aacaaaatca aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg
     5641 aatcggatca gtctattatc gagtaaattg gacgtctcca cccctgaagt gtatacggaa
     5701 caaggcgtac cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa
     5761 gagattgcta cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa
     5821 gcaagagaag tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa
     5881 atctatcaaa atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt
     5941 gcaaaaatgg ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt
     6001 tatcttgact ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca
     6061 gaagcggaag ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa
     6121 gcagctgaat tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa
     6181 ttagcaacct acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat
     6241 ttagaaagtg cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta
     6301 gaacgtcaaa aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat
     6361 gattcggaag tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa
     6421 gctcaaaaag tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg
     6481 gcagaagcgg aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta
     6541 gcaaaaggaa aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat
     6601 ggcgaagcag ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca
     6661 gcacagccgc taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga
     6721 gaaaccagtg gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa
     6781 gaaacactaa aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca
     6841 cgtgggacta ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg
     6901 atgaaggaag aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt
     6961 aaagttcttc tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt
     7021 cttttgtttt tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt
     7081 cttttggagt tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg
     7141 aagaaagaaa ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg
     7201 gatagcatac aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc
     7261 tattgcggaa atgataccgg aattattgtt aataccatct gacacgacga tcacaataat
     7321 aactagaata ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac
     7381 aaaaaaataa atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc
     7441 aaaagaaacg gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat
     7501 catcgggatt cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc
     7561 acttttgatc gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt
     7621 tggtcttttt actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt
     7681 ataattaaaa taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt
     7741 gatcagtgtc ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc
     7801 tgcagggatc attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg
     7861 catgaatcga atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc
     7921 tgttgggtat actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc
     7981 tcgcttcaaa ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt
     8041 ggaggaaaac atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt
     8101 agtatttgct ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa
     8161 ccaaatacag atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt
     8221 atcagaaaat aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga
     8281 aagccaagaa gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta
     8341 cgaaacttac ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa
     8401 tctagagaga atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta
     8461 tactgcagaa acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga
     8521 agtttatgta ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg
     8581 agcagaattg ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt
     8641 ctcccaacaa aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag
     8701 ctgatttcac aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca
     8761 caatattcaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga
     8821 acggtaggct cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa
     8881 gtccagccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
     8941 cagaacagct ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga
     9001 tccaagattt cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca
     9061 atcatgtgta ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg
     9121 atgaattcct ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca
     9181 gcaatcgtca cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga
     9241 aaagtcaagc tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt
     9301 tcgtccacgc ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc
     9361 aaaaagaaac gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct
     9421 aattctggat tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct
     9481 tggatcattt ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa
     9541 agtggcatgt tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag
     9601 cgcatagcgt cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt
     9661 ttgcctgctt ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg
     9721 tacacgatgt cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga
     9781 atattgtaac ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt
     9841 agttcgctcg tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca
     9901 acgattcccc attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc
     9961 ttgtctgtct attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat
    10021 taaaaaaaat gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag
    10081 ttaaaaggag gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt
    10141 agaacagttt gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca
    10201 atggcaaaaa tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa
    10261 aaatcttttc actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat
    10321 ttttcccgaa aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc
    10381 gcaaaaatgg cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca
    10441 gcaggctggt agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga
    10501 tgatattttg aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat
    10561 cgaacttctc gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg
    10621 ggaacgtgaa gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag
    10681 ttgtgattat gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc
    10741 aagccggcaa tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga
    10801 acagtcaaac aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac
    10861 aattgccaga tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat
    10921 tagaaaaaga aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg
    10981 acaaacgcat tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc
    11041 cattagtgat ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa
    11101 atgtccaaga tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta
    11161 aagaaacgat tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg
    11221 tcgatcaaaa ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc
    11281 agcctggagc taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc
    11341 caaaaggaca cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg
    11401 gtaaagaaaa cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg
    11461 aattaaatga aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca
    11521 agaatatccg ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt
    11581 tttccttgaa gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat
    11641 cccatcagca atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg
    11701 gagtgcttcc tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta
    11761 ccttggctag caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag
    11821 taagcctgga aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca
    11881 aagtgtatga ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag
    11941 tagattggaa tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg
    12001 cacaagcatt gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc
    12061 aggtcatcga gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct
    12121 gatggcagaa caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt
    12181 accggaaatc attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat
    12241 catctttact acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt
    12301 gtatcctttt acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc
    12361 agttttctcc ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat
    12421 gttcgcagaa gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac
    12481 agtaaaagaa gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata
    12541 tacagaaata gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa
    12601 agaaactgca gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc
    12661 gacaggtggt ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat
    12721 cacatatacg ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga
    12781 aactgagaaa aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag
    12841 tggctcttcc tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa
    12901 atttttttgt ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt
    12961 agcattctaa caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga
    13021 ataaaaacta aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa
    13081 gtcagcggga actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat
    13141 tgtacctaat ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca
    13201 cgaagaagca tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt
    13261 ttctttgcgt aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag
    13321 aatcagcact atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata
    13381 aggcaagtct tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata
    13441 aaaatacagt atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa
    13501 tgagaatcat cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag
    13561 atgacaaaaa aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa
    13621 cagtaaaagt aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat
    13681 tttatgatta tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct
    13741 gggacatttt ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag
    13801 atgaatatgt ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa
    13861 cgacgactta cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg
    13921 cttacaataa aaatatattt gaacaattaa atatccctga accagcttca aattggacat
    13981 gggatgaatt tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt
    14041 caagattcga agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat
    14101 tgaattttta taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc
    14161 taacagattt tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag
    14221 gtggtggagc tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg
    14281 caatggtgtt tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata
    14341 tggagattca gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt
    14401 tacgttcttc gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta
    14461 agtttattga tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg
    14521 gtgtaccaat catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc
    14581 aagcgacatt tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct
    14641 ttgatagtgc tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca
    14701 tttatgaaga agtaacagca gaagaagcag cagagagtct ttatgagttt gcgtcaaaca
    14761 ttctaggata gcagcaggag gaaggcatga aaaaagaaaa actaaaacgg ttcttggatc
    14821 aagacaatgt ggtcggttac gtatttgctt ctccattcat cctaggattt ctattgataa
    14881 cagtgatacc gatgatcttg tcactttatt attcgttcac cgattatcgt ttgggaaatc
    14941 cagtctcatg gatcggcttg gataattata taaagatgct gagtgatgcc cgttttttga
    15001 actccatccg agtcactttt ctctatgtat ttgcatctgt acctgtgaaa ctgatctttg
    15061 gtctattgat tgcttatctt ttaacgagga atatcaaagg aacgactttc tttcgttctt
    15121 tgtattatgt gcctagtttg atcggtggca gtatttctat cgctctagtt tggcgggagt
    15181 tgttttctaa aaacgggtta gtgaatattt tcctggaacg attaggcgca cctgcgcttt
    15241 cctggtttag cgatcaaaaa ctggtcatga tccctcttgt tttgatgtct gcttggcaat
    15301 tcggttcggc aatgatcatc tttgctgcag gtatcaaaga gatcccaggc tcgtattatg
    15361 aagcggcaga gatcgatgga gcgaacaaat ggcagacgtt ttacaagatc acgttacctc
    15421 ttctttcgcc gatcattttg tataacttgg ttatgcagac aatcgcggcg tttatgactt
    15481 ttacgcaggc atttatcatt acccaaggtg gtccgaatga tgccacgaac ttttatgctc
    15541 tttatgtgta taaccaagct ttttctttta attctatggg gtatgctagt gctatttcgt
    15601 ggatcatgct gttgattatg ggagctgtca cctttctgat tttcaaatca tcaaaaaaat
    15661 gggtcgttgt gtgaatgaga ggaggaagaa atgaataata cagtaacgaa atggtttcaa
    15721 tacggtttta ttttgctatt aggtctagtc atgatttatc cggtcttatg gatgatcgca
    15781 ggttctttta aaacggatca agagattatg agtggttcgc tgaatttgat tccagagact
    15841 tttcaatggg aaaattacgc aaaaggatgg gcggggtttg ctggaatttc attttttact
    15901 ttctttagga attcgtttgt catctctgca gtttcgacga tagggacagt tctgagctcg
    15961 acttgtattg cttacgcatt atcgagaatc gatttcaaag gaaaaagatt ctggttcgtc
    16021 gtcatgttag taacgatgat gattcctgca caagtgattt tgattccgca gtttattatt
    16081 tacaatcggc tgaatttggt tgggacgtat gtgccgctga ttttaccaca tttttttgga
    16141 caagcatttt tcatttacca gatcatgcaa ttcatggtca gcatccccaa agagttagat
    16201 gaatcagcga ttattgacgg atgcagcaag tatagtgtct ttaccaaaat tatttttcct
    16261 ttgctgaaac cttcgatcat cacgacgatc atcatccagt tttattggaa atgggacgac
    16321 ttcatgggac cattgattta tttaaacaag ccgagatctt acacggtatc gattgccatc
    16381 aaattatttg cagacgctgg atctagtacg acttacgcga gtatgtttgc gatgtctacg
    16441 ttgtcgctgc taccggtttt tctgattttc ttattcttca ataaatatct tgtccagggg
    16501 atcagtacaa gcggtctgaa gggctgaata tgaaaaagcc aatagaagag atgtttcttt
    16561 acaagctcaa ctactatgtc tgtcggattt ttttaaccag tcttatcagt aatgctcttt
    16621 tcgctttagt tctaggatgt ttgttcactt taggattagt gctgtctgct cgtgttgtct
    16681 atctgattta ttttttgatg atgtatcctt cttattatct atctgtccat tacttggcag
    16741 aaaaaaaggt atgttggagg aaaggaaaaa tcaaacggat gtgtttcatc attacgttgt
    16801 ttcagctgtt tgtcattatt tttgcagttg attggtattt attagtggga aagattggag
    16861 cttggtatct gattcctttt ctattgtgtc tgttgctttt tgtatcccat agttttttta
    16921 gtacgctaat tgatatctgc aaagaagata aagcagatag tcttgtttat ttgatcaaaa
    16981 ggaatatctg gctgttgtca aaaaacgtca aggggtttct gtggtattta tgccttttgt
    17041 atggactgtt ctggtgtata cagcatgatt ttttactcac tgcattactg tatccagggc
    17101 tgccatttgt cacagtattt tgcttaaaag aaaaggagaa tgactttaaa tgaagaaaaa
    17161 atatgtacaa gtcggacttg gtgggagagc gcgttttttt tatgaggcaa ttgcctctac
    17221 gtatcatcaa acttgtgaac tagtcggttt ttgtgatgta tctcaaacaa ggatggatta
    17281 tgcaaatcgg atacttgtga acaagtatgg acagaaaaaa gtaaagacat gtatgccatc
    17341 acaatttgag caactactgg atgacgtacg gccggctgaa gtcattatta cgtcagtaga
    17401 tcgaacgcat catgattata tcattcgtgc aatgaaaaaa ggatatgatg ttatttgcga
    17461 aaaacctctg actatcgatg aggaaaaagc tcaagaaata ataacggtcc aaaaacagac
    17521 gggaagaagc ctgcgagtca cttttaatta tcgttatgca ccgtatcaca cgaagataaa
    17581 ggaactgata gaaagtgatg tgattggtga tgtgttttct atccacttcg aatggctgtt
    17641 aaatacgcaa cacggggcag attatttcag aagatggcat cgaaataagc acaatagcgg
    17701 cgggcttctt gttcataaat ctacccatca ttttgatcta gtgaattttt ggttgaacag
    17761 ccgtccccaa aaggtatttg cattcggtgg attaaacttt tatgggatgc ataatgcgga
    17821 gaaacgcgga gtagaaatat tttacagtcg atgctatgga agcgaactag ctaaagctga
    17881 tccatttgct atagacctgt cagccaatga ggaattgcga gaaatgtatt tagaagcaga
    17941 aaaagacagt ggatatattc gtgaccgcag tgtattttcc gatgatatct ctattgaaga
    18001 tacactaggc gtaacggtca aatatgaaac tggtgcgatt ttgacgtatt cgctaaacgc
    18061 gtatatgccg tgggaaggat tcaatgtttc tttcaatggc agcaaaggac ggatcgaaat
    18121 gaaagtgatc gaaaagtctt acatcaatgc aggtggagat aaaaaggatg aaggagcagc
    18181 cgttcttcag tccatccatg tctatccgat gttcggtgag ccatatgagg taatcgtcga
    18241 acaagaagcg ggcggacatg gcggaggaga tccgcgttta ttagatgatc tatttggtga
    18301 agaaaaagaa gaagatccgt ggaatcgggc agcaactcac gtagatggta ttttgtcgat
    18361 tttgacagga atcgcagcaa atcattctat tgcttcagga aaagcagtag tgattgacga
    18421 gcttgtcagc ttaaaagaat aagaatagat ttacggaaat tacttcccgt agtttataaa
    18481 atcttgcctt attgccgata ggctgtcttt tgaacactat ttattcggaa aaaggggcta
    18541 tgacatgctt tttgtcatgc cttcttttta tgtgggctgc ttttttagat aggagaaaat
    18601 aaaaataact atttaaaccc gaacaaaaag gaataatgca ttaaaaatca ttagatttta
    18661 caaaaaaata agtaaaccac gttgtcagtt tcagaaagta gtgttagact gttttcaata
    18721 tttgaaacaa cattttttat tcaaatatgg caaactgcct tcacgtgcaa aacagttgca
    18781 agaaaatgaa tttacactat tattagtttt acatacctac ttatatatcg tcataatatg
    18841 gatgacagtt tctagccagt accgtaaatg ctggactata agtaaaagat tggctatttc
    18901 acccttcttt tcttatgata aagtggaggg tgtttttctt taacagaaac atagtaggtt
    18961 tttgcacaaa agcggttatt tcggtattaa taaatgataa aggagcaaat aaagtgaaag
    19021 aattagttga acgtattcaa aaagacggac gtatattagg cgagggcgta ttaaaagtcg
    19081 acagcttcgt cactcaccaa gtagaccctg tattgatgga acaaatggga aaacggtttg
    19141 cagaagtatt taaagaacaa ggaatcacga aagttgtgac cattgaagct tctgggatcg
    19201 caccagcttt atttgctgca agagaattag atgttccaat gatcttcgct agaaaagcaa
    19261 aaagtttgac gatggatgaa gaattattga cagcttctgt ttattctttt acgaaacaag
    19321 tgacaagtac gatttctatt tctcgtaaat ttttatcttc agaagacaaa gtattgatta
    19381 tcgacgattt cttggcaaac ggacaagcgg caaaaggatt gatcgaatta tgccagcaag
    19441 ctggagcgca agtagaagga atcgggatcg tcatcgaaaa gtctttccaa gatggacgtc
    19501 agttacttga agaaatggga ttgagagtcg tttctcttgc gcgtattgct tccttgacga
    19561 atggacaagt tgagtttctt gaggaggacg cataatcgtg gaaaaagaaa cacaaaatgg
    19621 aaaagctgcg gttttagggc tgcagcatct gctagcgatg tatgcaggag cagtagcggt
    19681 gcctcttttg atcggtacag gactaggctt taatcaagaa cagatgactt atttgatttc
    19741 aattgatatc tttatgtgtg gattagcgac attattgcaa ttgacagtta accgattttt
    19801 cggaattggc ttgcctgtcg ttttaggatg tgcgatccaa gcagtagctc cactgatctt
    19861 gatcggaacc aatgaaggag taggagctat ctacggatct attatcgctt ctgggatctt
    19921 tgttgtgtta gtttctggtg tcttttctaa aattaaaaaa ctattccctc ctatagtaac
    19981 aggaacggtt atcaccgtta ttggtttgac tttgattcct gtagcaatcg agaaaatggg
    20041 tggaggtaat gcaacagcag ctgattttgg tgacaaaaca aatctgctac ttgcatttgt
    20101 gacgattctc ctaattgtag gcgtgcaaat gctggcaaaa gggttcattc gttcaatcgc
    20161 ggtattgatt ggtttagtcg gaggaagcat cttagctgct ttcatgggaa tgattgatct
    20221 gagcgcgatt ggagatgcgc cgttgttcca tgttccacaa cccttttatt ttggtaaacc
    20281 aactttcgac gtatggtcga ttttactaat gatcatcatt tccatggtca gcatggtaga
    20341 atcaacaggt gtatattttg cacttggtga cattactggg aaaaatgttg gagaagaaga
    20401 attgaaaaag ggatatcgtg cagaaggact tgccgttatc ttaggcggta ttttcaatac
    20461 attcccttat actggctttt ctcaaaatgt cggattggtt caactttcgg gaatcaaaac
    20521 aagaaaaccc atctatttct ctgcattctt cttgattgtt ttaggattat tccctaaaat
    20581 cggtgcgtta gcacaaatca tccctgagcc ggtacttggt ggaggaatgt tagtcatgtt
    20641 cgggatggtg gcagtccaag gaatccgtat gctgttggaa gttgatttca gcaatgataa
    20701 aaatctattg attgttgctg tttctgttgg atttgggctg ggtttcaatg ttacgccgac
    20761 attattcagt caaatgccgc aaacattgca aatgttcact ggaaatggca tcgtaatgag
    20821 tagcattacg gctatcatat tgaatttgat ctttaatgga ttgaaaaaag atgaagaaat
    20881 attggaagaa gccttcaaga agtaatagag caaaagaaag gttgtgaaat cagcgttttg
    20941 acctgattgt tagagaaaca aacggaaaaa tagttcctcg tattttgccg tttgtttgag
    21001 ctaataccga gggtcagctg attgaacacc gttgaaggaa gaggtcgtga gcctgccgtt
    21061 catctccgag tcacaaggaa caatttcaaa aaaaagcttc tcatgtttgt tgaatattgt
    21121 tccttgtgac cgaggagttg gcaggagaac accgtttatc cataaaaagg aatgtgacct
    21181 aacttttgtc acattccttt tttttatctt taataaacac gaacaataaa atgtttttct
    21241 ttgttaacat tcgaaatttc tttgacatca aaaaaagtga ttgttaaaat atgaatgtag
    21301 aaaaactggg aggaaaaaca atgacagcaa aaatctctgt gattatgggg agtacttcag
    21361 attgggaaac aatgaaagaa gcatgtcaaa tattggatga attcggtgtg tcttatgaaa
    21421 aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt tgaatatgca caaaacgctc
    21481 gaaaaaaagg aatcaaaatc attattgcag gagcaggggg agccgcgcat ctgccgggta
    21541 tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc ggtccaatca cgaacattaa
    21601 atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg aggagtacct gtagctacta
    21661 cagcaatcgg aaaagcggga gcaattaatg ctggactact tgcgatacag atgctttcca
    21721 tgtatgattt agaattggag gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg
    21781 ttatagaaag tagtgatcaa cttggttaaa cctttgttgc ctggttcaac gatcgggatc
    21841 gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg caaaagaaat gggctttaaa
    21901 gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac aagttgcaga ttggcatatt
    21961 gaaagtgtct atgatgacac ctttgcgctg aaagagcttg ccagaagaac agatgtcatt
    22021 acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg cgattttacc catgtcattt
    22081 attcctcaag ggacagactt gcttgcgatt acgcaagacc gcctgttaga aaaatctttt
    22141 ttggaaacga ataacatcgt gattgctcct tatgcaacga tcgtcagtcc gacggatatc
    22201 caagatgcga ttgacgggat cggctatcct tgtgttttga aaaccactcg aggcggctat
    22261 gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc tagctcctgc catggacctt
    22321 ttaagagaag gaacctgtgt tttagaagca tggatcccgt ttgaaaaaga gatttcgatc
    22381 atggtggcag gaaatggtca aggagatttc acgacttttc cggtcgtgga aaacatccac
    22441 cacaacaata ttcttcatga aacgattgct ccagccgcga ttgatcagga tgtgatcgag
    22501 gaggctgagc gtattgcccg tgtgattgca gaagcagtga gcctttcagg tgttttaggg
    22561 gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga atgaattagc tcctcgtcct
    22621 cacaattctg gacattattc tattgaagct tgttctatga gtcagttcga tgcccatatt
    22681 cgaggtattt gtggttggcc attaggagaa aaagtacgtt tgataagcaa tgcagtaatg
    22741 gtcaatgttt tgggagaaca actgctggaa agctatcgct tgattgagaa taagccagac
    22801 tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc
    22861 actatcttga cagaagacat ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg
    22921 gattagagga gaaaaaacat gttagaacgt tatacacgcc ctgaaatggg gaatatctgg
    22981 acggaagaga acaaataccg tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg
    23041 gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt
    23101 gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca
    23161 cgagcggttt ctgaaacatt gggagaagaa agaaaatggg tccattatgg tctaacaagt
    23221 acggatgtcg tagatactgc ttatggctat ctcatcaagc aagcaaatga tattttacgg
    23281 gaagacttga aacgttttac ggaaatcatt ggcgaaaaag caaaagaaca caaatataca
    23341 gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa ctacttttgg attgaaactt
    23401 gccacatggt attcagaaat gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga
    23461 gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg caaatatccc gccatttatt
    23521 gaagagtatg tatgtaaaca attggggatc cgtccacaag aaatttctac tcaagttctc
    23581 cctcgggatc tacatgctga atatttctca gcgatggcgc ttattgctac aagtattgaa
    23641 cgttttgcaa cagagatacg cggattgcaa aaatccgaaa cacgtgaagt agaagaattt
    23701 tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca aaaggaatcc gatcggttca
    23761 gaaaatatgg ctggtctagc cagagtggtg cgcggacaca tggtcactgc ctatgaaaat
    23821 gtcacattat ggcatgaaag agatatttct cattcttcag cagaacgaat catcttgcct
    23881 gataccacga ttttattgaa ttacatgtta aatcgttttg gcaatatcgt taaaaatcta
    23941 acggtctttc ctgaaaacat gaagcggaat atgtctgcaa catttggttt gatttatagc
    24001 cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa gagaagaagc ttatgatctt
    24061 gttcagccaa aaactgcgta cgcttgggac aaccaagtgc agttccgccc gctgttagaa
    24121 gaagatccaa ccatcacatc ggtattatct aaagaagaaa tagacgatgc ctttgattat
    24181 catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag taggtttgga ataaagaaga
    24241 gtgagttgca aaattcaatt aaaatagaat agcaaaaata accaaaaaag aagcagatcc
    24301 gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag gaacctgatt
    24361 ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag gaattaagcg
    24421 taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag caagttttcg
    24481 agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata atgatttttg
    24541 aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc tgtctgataa
    24601 agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat ttttcggaat
    24661 gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag caaatcatta
    24721 tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc ttgttttatt
    24781 gcatcttcca tcggcaagtg cctcctcgaa cccattaacc aaatttttag ttaattctaa
    24841 tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt tcgtttctga
    24901 taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata
    24961 aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt
    25021 aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag
    25081 ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag
    25141 caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc
    25201 aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg
    25261 aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg
    25321 ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag
    25381 aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat gatccattta
    25441 tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc agtgagctga
    25501 aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg
    25561 ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg
    25621 aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag
    25681 acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc
    25741 tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac
    25801 ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat
    25861 gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga
    25921 tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt
    25981 aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg
    26041 cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga
    26101 tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg
    26161 gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc
    26221 ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg
    26281 gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt gtactgagac
    26341 gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg
    26401 cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag
    26461 gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct
    26521 ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag
    26581 cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat
    26641 cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat
    26701 agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg
    26761 aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc
    26821 ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa
    26881 attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg
    26941 gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa
    27001 gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa
    27061 tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt
    27121 tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg
    27181 gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt
    27241 gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata
    27301 tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag
    27361 gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac
    27421 agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt
    27481 gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag
    27541 caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga
    27601 cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg
    27661 aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac
    27721 tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat
    27781 atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag
    27841 atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt
    27901 tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc
    27961 aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca
    28021 atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag
    28081 cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag
    28141 gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg
    28201 cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat
    28261 cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag
    28321 gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg
    28381 gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag
    28441 gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa
    28501 agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga
    28561 gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact
    28621 gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga
    28681 tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg
    28741 tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg
    28801 ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga
    28861 tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga
    28921 agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc
    28981 tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat
    29041 cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt
    29101 attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg
    29161 ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca
    29221 tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa
    29281 agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca
    29341 tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga
    29401 agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga
    29461 cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc
    29521 ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg
    29581 tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca
    29641 gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg
    29701 gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt
    29761 aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc
    29821 ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac
    29881 atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt
    29941 acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac
    30001 gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag
    30061 gattgcttct cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa
    30121 agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc
    30181 tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc
    30241 gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta
    30301 tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa
    30361 gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag
    30421 ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta
    30481 tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag
    30541 agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag
    30601 cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag
    30661 cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt
    30721 tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc
    30781 tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag
    30841 ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc
    30901 caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg
    30961 ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga
    31021 aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc
    31081 cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag
    31141 aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc
    31201 cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt
    31261 atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag
    31321 aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg
    31381 ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc
    31441 ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga
    31501 atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac
    31561 tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta
    31621 tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg
    31681 atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat
    31741 ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc
    31801 tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc
    31861 aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga
    31921 aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa
    31981 tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta
    32041 gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag
    32101 catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct
    32161 gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga
    32221 agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac
    32281 tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata
    32341 gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa
    32401 aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa
    32461 ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt
    32521 cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa
    32581 aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa
    32641 aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca
    32701 gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct
    32761 gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat
    32821 ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca
    32881 gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca
    32941 gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa
    33001 gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag
    33061 gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat
    33121 caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact
    33181 ccagcagaag caaaagcatt agtgtttgct tggagagctg tcaaacatgt gaaatctaat
    33241 gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga
    33301 gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta
    33361 gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata
    33421 aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat
    33481 aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc
    33541 ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa
    33601 cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca
    33661 aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt
    33721 gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg
    33781 atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct
    33841 ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca
    33901 gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt
    33961 gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc
    34021 acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat
    34081 agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg
    34141 tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt
    34201 gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt
    34261 tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg
    34321 aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa
    34381 ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt
    34441 tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta
    34501 gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac
    34561 cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt
    34621 tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat
    34681 ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta
    34741 atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat
    34801 agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa
    34861 tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt
    34921 atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc
    34981 agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat
    35041 tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg
    35101 tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct
    35161 ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca
    35221 tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt
    35281 acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga
    35341 attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat
    35401 cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt
    35461 gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat
    35521 cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg
    35581 gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga
    35641 attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc
    35701 ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt
    35761 agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta
    35821 cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa
    35881 gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg
    35941 tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt
    36001 tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa
    36061 ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac
    36121 gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat
    36181 cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg
    36241 caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa
    36301 aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag
    36361 cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga
    36421 ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt
    36481 tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag
    36541 ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata
    36601 atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt
    36661 cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta
    36721 tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa
    36781 tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta
    36841 cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga
    36901 atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat
    36961 aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata
    37021 cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac
    37081 tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa
    37141 caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt
    37201 ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta
    37261 cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag
    37321 tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt
    37381 ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac
    37441 catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt
    37501 cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg
    37561 tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg
    37621 tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg
    37681 tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct
    37741 ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat
    37801 tggatcatct catcaatttt gcatgtggta atacgaataa atcctttgtg acggacgtac
    37861 ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa
    37921 acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt
    37981 tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct
    38041 tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct
    38101 tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa
    38161 tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc
    38221 aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta
    38281 agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa
    38341 agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag
    38401 cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt
    38461 aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca
    38521 cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg
    38581 atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga
    38641 taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt
    38701 gtatttgtat tatttttgct ctctcgaata tcattcctaa gttcttctag gatattttcc
    38761 tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact
    38821 ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga
    38881 taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat
    38941 agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg
    39001 atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac
    39061 agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata
    39121 acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt
    39181 gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat
    39241 caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat
    39301 ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg
    39361 tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt
    39421 catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga
    39481 ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc
    39541 taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc
    39601 ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt
    39661 cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa
    39721 tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc
    39781 aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag
    39841 aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca
    39901 gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt
    39961 attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac
    40021 acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg
    40081 aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga
    40141 caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct
    40201 ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa
    40261 tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt
    40321 ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa
    40381 tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt
    40441 tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa
    40501 aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta
    40561 actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta
    40621 ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta
    40681 tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg
    40741 attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg
    40801 atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg
    40861 agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat
    40921 aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat
    40981 ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa
    41041 cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc
    41101 ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa
    41161 atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga aacttatgat
    41221 aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat
    41281 tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag
    41341 gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt
    41401 gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg
    41461 tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa
    41521 aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa
    41581 tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca
    41641 tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt
    41701 ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa
    41761 catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga
    41821 ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc
    41881 tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga
    41941 gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga
    42001 atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga
    42061 cactagaaaa tgaaatgatc agcgaagagc atattattcg cggaatggtg ggcagagatt
    42121 tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag
    42181 actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc
    42241 atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat
    42301 tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg
    42361 aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg
    42421 tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga
    42481 cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa
    42541 ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg
    42601 tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac
    42661 aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg
    42721 agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt
    42781 ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta
    42841 ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga
    42901 aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa
    42961 ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta
    43021 gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat
    43081 ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta
    43141 gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca
    43201 attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt
    43261 cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata
    43321 ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt
    43381 ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat
    43441 ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc
    43501 atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta
    43561 tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta
    43621 ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt
    43681 ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt
    43741 tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc
    43801 acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct
    43861 gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg
    43921 gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt
    43981 gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta
    44041 gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat
    44101 aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa
    44161 gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga
    44221 ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt
    44281 ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg
    44341 ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga
    44401 agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac
    44461 ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat
    44521 tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg
    44581 gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac
    44641 ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga
    44701 atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat
    44761 tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt
    44821 tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg
    44881 gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg
    44941 gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg
    45001 tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat
    45061 gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg
    45121 aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa
    45181 tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga
    45241 aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat
    45301 tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa
    45361 aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc
    45421 caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc
    45481 ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat
    45541 gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata
    45601 gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat
    45661 cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt
    45721 caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc
    45781 catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg
    45841 tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg
    45901 atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag
    45961 atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg
    46021 aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat
    46081 cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa
    46141 ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg
    46201 attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt
    46261 taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa
    46321 gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg
    46381 cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa
    46441 aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg
    46501 gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat
    46561 gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg
    46621 ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg
    46681 atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa
    46741 atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat
    46801 tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg
    46861 gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt
    46921 gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg
    46981 gatgaaaacc aaataccgtt gttttattca aagatgaaaa ttctagaaaa gtggttaaca
    47041 gaataatttc acaaagtaaa tagattaaag aatataacag cttggattta aatttcaagc
    47101 tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt
    47161 gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca
    47221 acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat
    47281 gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt
    47341 ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca
    47401 tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg
    47461 cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa
    47521 agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca
    47581 agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga
    47641 tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac
    47701 agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag
    47761 tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga
    47821 tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg
    47881 acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat
    47941 cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa
    48001 aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc
    48061 aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta
    48121 tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt
    48181 cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt
    48241 atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact
    48301 gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa
    48361 agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac
    48421 ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga
    48481 aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt
    48541 gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc
    48601 gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta
    48661 gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac
    48721 ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc
    48781 gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga
    48841 tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta
    48901 gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca
    48961 attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa
    49021 ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca
    49081 aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg
    49141 aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat
    49201 aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg
    49261 cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt
    49321 gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact
    49381 ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt
    49441 tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca
    49501 cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat
    49561 atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc
    49621 atggcttatc aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc
    49681 aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa
    49741 aatgacaaat cagtagaaga aatcccgtca cctgttacag gaacggttaa aaatgtgatt
    49801 gttccagaag gtaccgtagc aaatgttggg gatgtattag tagaaatcga tgcaccagga
    49861 cacgaagaca atgaagggga cagtggtgta gccgcagaat cccaaactcc agctaaaccg
    49921 gctgctgaac ccactgttga tacagaatca gctggatcgt caagcgaagg agtcttccaa
    49981 tttaagttac cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc
    50041 aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca
    50101 gtagaagaaa ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt tccagaaggc
    50161 actgtagcaa atgttgggga tgtattagta gaaatcgatg ctcctggaca caatagtgca
    50221 ccgtcaacta gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac atcaggttca
    50281 gcaagtgtcg ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc atctgttcgt
    50341 caatttgccc gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc
    50401 agagtaacaa aagaggacat cgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg
    50461 aaatcagaag cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa
    50521 ccagctgcac cagcaaaacc attcaagtcc aacttaggag acttggaaga acgtgtagca
    50581 atgacgccaa cacgtaaagc tatcgctaaa gcaatggtca acagtaagca tacagctcca
    50641 cacgtgacat tgcatgatga agtagaagtg tctaaactat gggataatcg gaaacgattt
    50701 aaagaagttg ctgctgcaaa tggtacgaaa cttactttct tgccatatgt cgtgaaagct
    50761 ttaacagcga ctgttaagaa atacccagta ttgaatgcat ctatcgacga tgcaaaccaa
    50821 gaaatcgttt ataaacatta ctacaatatc ggtattgcaa ctgatacgga tcacggacta
    50881 tatgtaccaa atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc
    50941 aatgagaaag caaaattagc tcatgacggc aaactatctg cagaagatat gcgcaatgga
    51001 acaattacga tcagcaatat cggatctgta ggtggcggtt ggtttacacc agtcatcaac
    51061 tatccagaag tagctatttt aggtgtagga acgattgcac aacaaccaat cgtaaatgct
    51121 gaaggcgaaa tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc
    51181 gtagatggag caactgcgca acaagcaatg aataatatca aacgtctttt agctgatcca
    51241 gagctattaa tgatggaagg atgataaaca aatggtagtt ggagattttg ctgtagaatt
    51301 agatacagtt gtaattggtg cgggacctgg tggctatgta gctgccatcc gcgctgctga
    51361 aatgggacaa aaagtagcaa tcattgaaag agagtatatt ggcggtgttt gtttgaatgt
    51421 cggctgtatt ccttcaaaag cactgatagc tgcaggacac cattatcaag agtcactaga
    51481 ttctgaaatg tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga
    51541 atggaaagaa aacaaagttg tccatacgtt gacttctggt gttggtttcc tattaaaaaa
    51601 acataaggtt gaaacaatcg aaggagaagc gttcttcgta gatgatcaca cactacgcgt
    51661 cattcatcca gattcagctc aaacttattc gttcaataac gcaatcatcg caacaggttc
    51721 ccgtccaatc gagattccag gatttaaatt cggaggacgt gtgttagatt ctactggcgg
    51781 attagcattg aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc
    51841 cgaattgggc ggagcgtacg caaacttagg agcagaagta acgattcttg aaggctcacc
    51901 acaaatttta ccaacttacg aaaaagatct agtaaaatta gtagaagatg atttcaagaa
    51961 aaaaggcgta acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag
    52021 cgtgacagtg aaatatgcag tggacgggaa agaagaatcc gttactgctg attatgtgat
    52081 ggtcactgta ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag cgggtgtcga
    52141 agtaggagaa cgaggattga tcacggtgga taaacaagga cgtacgaacg tacctaacat
    52201 ctatgccatc ggtgatatcg ttccaggggc ggcattagca cacaaagcca gttatgaagc
    52261 aaaaatcgct gctgaagcaa tctctggcaa aaaagtagct gtagattata aagcaatgcc
    52321 ggcagtagcc ttcactgatc ctgaattggc ttctgtaggt atgacgatca aagaagcaaa
    52381 agatgctggt atcgaagcaa aagcatataa attcccattt tcagggaacg gtcgggcatt
    52441 atcgcttggt aaaacagaag gattcatccg tctagttacg acgatcgaag ataacgtgct
    52501 tattggcgcg caaatcggtg gagtcggcgc aagtgatatg gtctctgaat tagctttagc
    52561 aatcgaatca ggaatgaatg ctgaagatat cgccttgacg attcatcctc atccatctct
    52621 tggcgaaatc gtcatggatg cttcggaatt agcattaggt ttaccaatcc atatttgatt
    52681 tgaataatac aggcgaaacg caactgaaaa atcagttgcg ttttgttttt tagaattatc
    52741 aataatatta ttatgtaaac ttaaaaatat gacaacttct tggtttatta ccacttccta
    52801 tgctacactg aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca
    52861 cttgatttag attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg
    52921 gaacgtgcga atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt
    52981 aaaactgttg tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct
    53041 tccggttatt ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg
    53101 agaatgaacg ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag
    53161 aagctggtga gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc
    53221 gaacggatct agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc
    53281 attcctttga tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg
    53341 atctcgatgg aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg
    53401 aaggggaaaa tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt
    53461 tcatttatga cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata
    53521 aaaatgatga gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg
    53581 tgaatagtta tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca
    53641 tggggacacg tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc
    53701 acattgctta tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg
    53761 aagagtttgg tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg
    53821 aatactattt agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag
    53881 aaggctgaaa aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat
    53941 tttctcgaaa aaatgaaaac attttactat atttcatgag aaaagtctgc tataattagc
    54001 agtcgagtaa aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa
    54061 ttatagaaac gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac
    54121 attagttgat gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga
    54181 acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa
    54241 cacagctgta gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga
    54301 cttcggtggc gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga
    54361 tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca
    54421 tatcacacca atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt
    54481 cgtagatgaa gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt
    54541 tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga
    54601 tcaagaacca accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt
    54661 cgataacagc gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt
    54721 aggtcgtatc ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc
    54781 attaattaaa ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt
    54841 tggactaaaa cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg
    54901 tatggaagat atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc
    54961 tatccttcac attgacgaac caacattgca aatgactttc ttagttaata attcaccatt
    55021 tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga
    55081 acttcaaaca gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt
    55141 ttctggtcgt ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata
    55201 cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga
    55261 accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc
    55321 cttgagctta cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg
    55381 tttgacattc ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat
    55441 gacacgtggt tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg
    55501 tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac
    55561 ttattctatc atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt
    55621 atatgaagga atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac
    55681 gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa
    55741 aaaaccaaaa caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg
    55801 cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga
    55861 aaaagcatct aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta
    55921 taaaaaatag ctggtttctt tttttgtcca actcttttct gtggagaagt catctttgtc
    55981 aatggacata aattcagaaa atacgccgaa agacgtttaa aagactttta aaaaacttat
    56041 ttctagagag tttttgtttc ataataagat aatgtttcac gaaaggagat caacttgtcc
    56101 ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa cgacttatta
    56161 aaacaatact ttggctatga tgagtttaga cctggtcaaa aagaaattat ccagaaagtc
    56221 attgatcagg aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc aatctgttac
    56281 cagcttccag ctttactatt agatgggctt actgtggttg tgtccccttt aatctctttg
    56341 atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc
    56401 tcgttagatg gttacgaaac agctagacgc ttccaagaaa tcgatagaca gcagtaccgt
    56461 ttactttata tcgctcctga acgttttatc atgccggatt ttatccaagc aatgaagcga
    56521 tggaacgtcc gtatgatcgc tattgacgag gcacattgta tctctcagtg ggggcatgat
    56581 ttccgcccaa gttatttaca gatggcgaac cagctagatc aactgccaaa tcgtcctgta
    56641 attgttgctt tgacagctac agcgactgtg caagtagctg ctgacataaa aagactttta
    56701 aaaattccag aaaacaacca tattcagact ggttttgaac gagaaaatct acgatttcaa
    56761 gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat atttaaagat caacaaaaat
    56821 caatctggta tcatttatgc agcaacgaga aaagaagtag accgtctcta tcatttgctt
    56881 agaaaattcg atttttccgt gggtagatat catggtggtc taaatgagaa tgagcgaaca
    56941 gaaatgcaag aagccttttt atacgatcgc ttgcagctga ttgtagcgac aaatgcattt
    57001 ggtatgggta taaataaaag caatgtccgt tttgttatcc attatcaaat tcctggctct
    57061 ctagaagcat actatcagga agctggtcgg gcgggaagag atggtctttc cagtgaagcc
    57121 atcctgcttt tctctcctca agatatccaa gtccaaaagt tttttatcca gcagtcccaa
    57181 agagaagaag ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac agaatacgtg
    57241 tatattgagt cctgtcttca gcaatacatc ctaaactatt ttggcgagac aagcagtccg
    57301 tgcaaccgtt gtggtaactg tttagatgac agagaaatag tagaagtaac gacagaagca
    57361 cagatggttc tttcttgctt aaaacgtatg ggtgagaact atggtaaaca aatgctgatg
    57421 aaagtcttag caggatcgaa agaacaaaag ctgcaagctt taggttttgg acatttatct
    57481 acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg
    57541 atatcgaatg gctatcttct gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa
    57601 agagggatac aagtattgaa aggacaagaa tctgtttatc gaaaggaacc caagaaagtc
    57661 cagcagctat cggatgaaga aaccgataca ctatttgaag tgctgcggga actgcgaacg
    57721 gatctagcat cagaagcagg cgttcccccg tatgtcgtat tttcagattc cacattgaaa
    57781 gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc ttcagataaa aggagttggc
    57841 caaagcaagc ttgataaata tggcgaagcc tttttatctc ggataaaaaa tgcggactct
    57901 aacgttctgg ataagtagaa agaacgaata cgtaaaaatg aataaaaaca actggttaaa
    57961 tctgtttttt ttgctcctta tgaaaatcaa atatgttata attaaggaat tagaatggga
    58021 ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag
    58081 cagaacggat caaacgatta atccgaaatc aaaacaacag tctctgtatc tcacaatgca
    58141 aagccttcga agaagtggta gatacacaga tgtacggctt ttcccggcaa gtcggttttg
    58201 ctgtccgtat aggagtaatc ggaaagaacg agggccagct gatgttaagt gagctagaac
    58261 gagaattgaa tcagttatat agtgaggttt accaagaaat ttatgataaa aaagaagttg
    58321 gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg catctattag actatagcat
    58381 cttgattccc tatttaattt tatgtgtatt gggattgatc atggtatata gttctacgtc
    58441 gtatctatta ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc agagtatttt
    58501 ttgggtgctt agcttgattg ctatcgcact actttataaa atgaaaacag atgttttgaa
    58561 aaaccagcgt ttgatcatgg cagccatcgc agttttgacg attttgctgc tgattgttgt
    58621 gttttttggt aaggagatca atggggcgaa aggttggctg caaattgcag gattctcgat
    58681 ccaacctgcc gaatacctaa aaatcatttc tatctggtac ctgtctctga ctttatctaa
    58741 aagacaaaac agtgtacaaa aagattttct tgggactgtg aaacgtccac ttgcaatggt
    58801 tatcggactt actgcattag tcgctattct tcccgatttt ggtaatgcgg cagttatctt
    58861 tttgattatt ttggttctgc ttttagccag cggagtcaat tatgtgtata ctttgattgt
    58921 aggagtagga ggcttttgtc taagtacttt tacgatctgg ctgatcaata tcacaaacgg
    58981 aaagatctta cccggacgtt tacagtatat ctataatcga tttgctattt accaaaatcc
    59041 tttctcagac gaattaaata aagggcatca actagtcaac ggctactatg ccatgttcaa
    59101 cggaggacta tttggcaggg gattaggtaa cagtatccag aaaaaaggat ttttacaaga
    59161 agcacaaacc gattttattt atgcaatcgt agtggaagaa ctgggagtga ttatggggat
    59221 tttgatatta gcgttgctat tctttatgat tgtgcgcatt gtccttgtag gtatccgttc
    59281 aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt
    59341 atttgtcaat cttggaggga ttactggcgt tattcctttg acagggatca cgttcccatt
    59401 tttaagtcaa ggaggctcca gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa
    59461 catcagtgcg gatgaaaaac gcaagagctt aggtttataa taaaaaaaga tgtaggagtg
    59521 gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg
    59581 cttgtacaga attaggaatt aaaactgttg gaatctatgc agaagaagat gaatattccg
    59641 tacaccgttt caaagcggat gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg
    59701 cgtatctaga tattgaaggg atcatttcta tcgcgaaaga atgcggagct gacgctattc
    59761 atccgggtta cggtcttttg tcggaaaact tgaattttgc gcaacggtgc gaagaagaag
    59821 gcatcatttt tgttggaccc aaactgcatc atttagatat ttttggagat aaaatcaaag
    59881 caaaagctgc agctatcgaa gcggggattg cttcgattcc aggcacagat ggaccaatcg
    59941 cttctataga cgatgccttg gagtttgcga aacaatacgg ttatccgatt atgatcaagg
    60001 cagctctagg cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag
    60061 agggctacga acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg
    60121 ttgaaaaata catcgctaat cctaagcata tcgaagtaca gattctcggg gatacgcatg
    60181 gaaacgtcat ccatcttttt gaaagagatt gttctgttca gcgccgtcac caaaaagtag
    60241 tagaagtagc gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag
    60301 ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg
    60361 aaggagatga tttttacttt atcgaagtca accctcgcgt ccaagtggaa catacgatca
    60421 cagaaatgat tacagatatt gatatcgtaa caacgcaact gttgattgct caaggtttgg
    60481 atctgcataa agaaatcgga ttgcctcagc aagaaggaat taaattaaat ggttcagcca
    60541 ttcaatgccg aatcactaca gaagacccgt tgaataactt tttaccagac acagggaaaa
    60601 tcgatacgta tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg
    60661 cgggctatgt agtgacaccg tattttgatt cattgctagt aaaagtatgt acgcatggtg
    60721 ctacctttga aacagccatt cagaaaatgg aacgttgctt gaaagaattt agaatccgtg
    60781 gagtgaaaac aaacattcca tttatgctca atgtcatcac acatcctgaa ttccaatcag
    60841 gaaatgcaaa aacaaccttc attgatagta cagcaacatt atttgaattt cctcgactac
    60901 gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat tactgtgaat ggttttccag
    60961 gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa gatctaatca
    61021 caagaactga ttacgtaacg gctaagaatg tattagatgc gaagggtgcc gatgctttgg
    61081 tcgaatggat caaaggacag gaaaatttgt tgttgacgga tacgacattt agagacgccc
    61141 atcaaagtct gctagcaact cgtgttcgga caaaggattt caagcagatt gcacgtttga
    61201 ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca acatttgatg
    61261 tggcttaccg ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa
    61321 tgcctaatac gttgctgcaa atgcttttca gaggttcaaa tgccgttggg tattctaatt
    61381 atcctgacaa tgtattggtg gaatttgtca aagaagcagc agcgcaagga atcgatgtat
    61441 ttcgaatctt tgatagtttg aactggactc cacaaatgga aaaaagtatc caagctgtac
    61501 gggatactgg taaaatcgca gaggcagcta tttgctatac tggcgatatc aacgatccaa
    61561 gtcgggcaaa atacaatgtt cagtattata aagacatggc aaaggaacta gaacaactgg
    61621 gtgcgcatat cattgcaatc aaagatatgg ctgggctgct taaaccgcag gcagcttatc
    61681 gtttgattag tgaattgaaa gaaacgacag atctgccaat ccatctgcat actcatgata
    61741 ccagcgggaa tggaatcatc acttattctg ctgcttcaaa agcaggagta gacatcgttg
    61801 acgtggcaat gagtgcaatg agtgggaata ctagtcagcc tagcatgagc agcttgtatt
    61861 atgcactggt caatggtcca cggctgccag aaatcacgat tgaaaatgca cagaaattaa
    61921 accattattg ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc
    61981 ctgaaacaga agtatatatg catgaaatgc caggaggaca gtattcgaat ctacagcaac
    62041 aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat caaacaaatg tatcatactg
    62101 tcaacttgat gtttggcgat atcgttaaag taactccatc atctaaagta gttggagata
    62161 tggcactatt tatggtccaa aatgatctta cagaagaaga tatttatgaa aaaggcgaaa
    62221 cactaagttt ccctgaatct gttgtgactt tcttccaagg agaactaggg caacccgttg
    62281 gcggatttcc taaaaaattg caaaagatta ttcttaaagg cagacctgca ttgaatgaac
    62341 gtccaggtct tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa
    62401 aaattggcta tgaaccaaaa caagacgaag tattaagcta tttgatgtat ccgcaagtct
    62461 ttttggacta tcaaaaagca tataatcaat ttggcgatgt tacattactg gacacaccaa
    62521 catttttcca aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa
    62581 cgcttatcat tcgtttagat gaaatcggag aacccgatat cgaagggaat cgcgtactgt
    62641 tcttcaattt gaatggacaa agacgtgaaa tcactatcaa agataattcg atcatcagta
    62701 cggtccaaac ccgattgaaa gctgaaccga caaaccgaga acaaattgga gcaacgatgt
    62761 ctggttctgt tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt
    62821 tgatgattac tgaagcgatg aaaatggaaa cggccatcga agcacgtttt gacggagaag
    62881 tcgcacatgt atatgtgtct tcaggagaca ctatttcgtc tggtgacctt ttgattgaag
    62941 tgactgagaa ataataagcg aaaggtgggg aaacatgaaa agactgggtt tgttttggct
    63001 gattctgcta ttagttattg tggcattcta catggaacca gtcttgtttc cgccaaaact
    63061 tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa atgacaacgt ggaaatacca
    63121 ggagcttaaa gcaactggtt tttctactta tatcggaaaa ccgatcgatc agctggaaaa
    63181 ggattttggc caagcctatg atcgattaac tagcggattt ggttttgaaa caagatatta
    63241 tgaaaataaa gctagtcatc tcacatttga agcgaacatc caagacagca gagtcacaac
    63301 tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg ttttatttag ggatgacgat
    63361 gcaagatctt acgaaaatca ctactatcta tactaatttc acttttgagt accatgatac
    63421 agaagttgga atcgaactaa tggaagagga tatgaattat cgtccgctaa ttgcttttga
    63481 taacgacact tttgctattt tattttttga tcaaacaaca aacggcctat tttcagttgt
    63541 gtatttagat aaagatagtc ttttgaaact attgccatat caagtgtttg gtgaaggcct
    63601 tccacattat caagcagata aatcggctga ttgggaatca atcaatcgcg ttaaagaacg
    63661 aaaaagtacg actttattaa atatgcttag aagagaagac gaacttccca tatacaatca
    63721 aacacttgcc tttcaaaacc acagtcagct gctactacat gattttttga caaggcctga
    63781 ggaaatattg tcagaggaac gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac
    63841 gacagtgaaa ttctcactga cgaatgatga attggagaag ttaatcagta aacaaaaatt
    63901 agaaaatact agtgggctat ttattcatcc ggcgattgac gctacttttt cgtttttata
    63961 ttggtacagc gatccttaca accatgaccg tttcatggac ccgggaacgg attctgtagg
    64021 cattgctttt tctaaagaaa atatgttagt cttattgcaa gaagaaaacg gagaaagtag
    64081 tgatagcagt gatcgttaac gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg
    64141 ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct tgaacataaa cagtcaatgg
    64201 aagaatctgc aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa gaagcatttg
    64261 aaaatgtagc ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa cgagctgttc
    64321 gaaaagcaaa aagacagctt gatatggatg agcatgttgc agctttccga tatagtgaaa
    64381 cacagctgca aagtctgttg gatcaaattg tccaagtact tgccgaagaa gtttctaatg
    64441 aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag
    64501 gtaattgtca tgcaattaga caatagtgaa tttgaaataa caaaacgacg cgggctgatc
    64561 gtatgggtct acagcttaaa acaattaaag aatctgaaaa agttcggctt gttgcattat
    64621 gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca
    64681 gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag aacgttctta ccgaccggat
    64741 atcgaaatga attttgccga aaaaatagga aacaaaacag tattaaaaga cgaaggtttc
    64801 gaaattgaag agctaaatac agaaatccga ttagctgaac atctttaaaa atctcttatg
    64861 agcagttttt aaataaaaaa taacttggat aacgatcgat ttttgatcat tgtccaagta
    64921 tttttttggt atactagtac cagtaccatt tctaaggagt gagtagatgc ggataatatc
    64981 aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca gaccaactac
    65041 agataaagta aaagaatcca tattcaatat gattggacca tattttgatg gagagacagt
    65101 attggatctg tttgcaggaa gcggtggttt agctatcgaa gcagtatcta gaggctgcag
    65161 tcatgcggta tgcgtagaca aaaattacca agcgctaaaa atcatcaaag aaaacatcga
    65221 aattacgaaa gaaccagaga aatttaccac aataaaactt gatgcggaca aagcaatcga
    65281 acggctagca gcggagaaac aacaattcga ctacttgttt cttgatccgc catatgcaaa
    65341 acaaaaaata atcgatcaaa tccataaaat ggaagaactg ggactttttt ctaaaaccgc
    65401 agttatcgta tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg ctggattcca
    65461 tcaaataaga aaacagattt atggcataac ggccgtaact atctatagaa aagagggaaa
    65521 tcaatgaaac gtgcattatt cccaggaagt tttgatcctt ttacaaaagg tcacttagat
    65581 acggtggaac gtgcggctaa attattcgat gaggtagtaa ttggtgtttt tatcaatact
    65641 tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat tgatcacaaa agctgtttct
    65701 catcttccta atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa
    65761 gagattggtg cagatgcgtt gatcagaggc atacgaagta tcaaagactt tgaatatgaa
    65821 cgagagattg ctcagatgaa tcatcatcta tatgatgaat tggaaactgt cttcttatta
    65881 gcaaaaccag aatatagtca cgtgagttcc agtattttaa aagaagtact gcactttggc
    65941 ggagatgtga gcagctactt accgccagtg atcaacgaag ctttagctgg aaagcgtgaa
    66001 tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt atcatatttg gaatgttgat
    66061 cattggattt gtagtagttc ccttgccgta ttatgtagaa tcaccgggag ccacgatcga
    66121 tctgaaagaa ctaattacgg tcaatgataa ggaagataaa ggttctgggt ctttctctct
    66181 tacctctgtt ggtatcaggc aagctacagg tttcttagct ttaaaagcta aaatgacccc
    66241 ttttgaagaa ttgatcagtg aagaagaatt gactggtggg gcgacaaatg aagaatataa
    66301 tcaaatgcaa cagtattata tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa
    66361 acttgcagac aagccttata aaatggaatt caaaggcgta tatgtcatga gtgtagagaa
    66421 aacatcgaat ttctttggca agctttcagt tggagataca gtaacaaaag tagacgggaa
    66481 aagctttcaa agtacagaag catttatgga ttatgtaaaa agccaaaaag ttggacaaac
    66541 aatcgaaatc agttatttgc ataatggtga agaaaaaaca gcttctggtg atctgatcga
    66601 gttaccaaca gataaaaagg caggaatagg tatcacgctt actgaccata ccgagatcga
    66661 atcagataca gatgttcgta ttaattctgg ctctatcggt gggccatcag cgggattgat
    66721 gttcacttta gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat
    66781 tgcaggaact ggaacgatca acagtgaagg agaagttggc cgaatcggag gaatcgataa
    66841 aaaagtggca agtgcggata aagctggcgt agagattttc tttgcaccag atgatacgat
    66901 ttcagacgat gttaagaaag aatatcctga aataaaaacc aactacgaag aagcaaaagc
    66961 tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc
    67021 gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt tgtgacacga
    67081 gttctgtcac aacccctctt tttcgtcaaa aatcttcacg ataaaaataa aaacaaaaga
    67141 aaaaattgac gtaattagat gttgaaggga cgattttgcc agagctcact gtttcttcaa
    67201 ttttaaatga aaggagtaat gaatgatttt tactggtcat tctttgaaaa aagaacaatg
    67261 atagaaaaaa tcagcaagaa aatactagct tcacccaaat tgatcatttg gatgtctatg
    67321 atagttctta tcttttgtat cgcaagtggt tttttattat caaatttgct attttataga
    67381 gaaacagaag agactgctgc aaaagaaata ctttcttcgt ttagttccag tggagaagat
    67441 ccatcagctg aaggaaacga tgtagaagag gatgctcgtc caaaggtcat gtacaccgat
    67501 atcaaaggaa gtgtgaagga gcctggtatc tattcctttt cttcagaaga acgggtctat
    67561 gatgtactaa agcgtgcagg tggattatta gaagaagcgg atagcgatcg aatcaacttt
    67621 tctgcgaaga tagaagatca gcaagtccta tatatccctg ctgtaggtga agagctgcca
    67681 gaacatcttg atcagttggc atcctcggaa ggaaaacagc ctacagcaga ttccgagcct
    67741 cccaaaataa atatcaatac agccactcca tcagaacttc agcagattcc tgggatcgga
    67801 tcagtcaaag cgcaagaaat cattcgattt agagaagaaa acggatcctt ccagaaagtg
    67861 gaagacctgc aggagatttc tggtatcggt gaaaagacag ttgaaaaact aaaaaacttc
    67921 gttacaatta aatagaatga ttcaaaatcg actggcacga gcaataaatg gacgctaatg
    67981 gatactaaga ataatccaat tctaagagct tggcaggacg aggaggtagt atttttggat
    68041 aggaaatagc ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga atgtctatct
    68101 ccaaaataaa atatctggga ttcatattat gaatacggct gtcagcggca ataacagtca
    68161 agctctacga gaacgctttg ccaaagatgt acttagctat acagcagata aagtatttat
    68221 tctcattggg acgaatgatc tagcagagca taaacaactt tctaaagaaa cgtaccaaaa
    68281 aatctgcagt ggatgatagt tcagctgaaa aaagactata gttctgatca tgctttcttt
    68341 atcacgccac cagcagtcga tgagacaaaa caaaaatatc ggaataacaa gttgatcaaa
    68401 gaatatatct ctgttctcac agaagtagca aaacgccatg attgtgaagt atcgattttt
    68461 atacggaatt tcttgaacaa atggattcga agaattttta taatgggcta atggatgacg
    68521 gacttcattt cggcagtgaa gggtaggatc ttttgtcaga caggatgatt cgttatattt
    68581 aaagaatatt ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa cggaggaata
    68641 taaaacatgt tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa agttactgat
    68701 aagtcacata atgaacgcat tccttgggac cagtacttta tggctcaggc agtactgtta
    68761 tctttacgta gtacgtgtac acgtttagaa gtaggagcga cactcgttcg agaaaaaagg
    68821 atcattgctg gcggctataa tggagcagtc agtggagatg tccactgcat agatgaaggg
    68881 tgttacgtcg tggatggaca ctgcttgcga acgatccatg cagagatgaa tgcactccta
    68941 cagtgtgcaa aacttggtat cccgacggaa ggttcagaaa tctatgtcac acattttcca
    69001 tgtttggcct gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa ttatctccat
    69061 gactatcgaa atgatcctta tgcccaagcg ttgatcgaac aattaggtgt atctgtacac
    69121 caagttgctt tagactcaca gcatttttca aaacttcaaa atgaattaac aggaaaacat
    69181 gtctagcgtt ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa ttacgtcgat
    69241 gattatctgt ttgttgtatt acactgacaa ttggggttat cgtttgcttc ttgccggaat
    69301 ttttttatgg ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta ttttgtgtag
    69361 cataacggta tccatttctt ataggctaca agagtattca tttttagagg aaaaaacaac
    69421 atctgcgcaa attactgtat ttacagatac gattcgtgca aatggtaatt ttttgacaat
    69481 ggaaggaagg ttatcagaga ataaacaaaa gctccagctt tcttatcaag taaacacaga
    69541 agcagaattg aaaacatggc ttaacttccg aggaaaggga atagaattga ctgctgaagg
    69601 cgagttcatc tctccagcaa aaaagcggaa tctttataat tttgaccaag aagcttactt
    69661 caggaaccat agaatttctg gacggttcca aattcaaaag attgaacaga tcaccatgca
    69721 aaatcactgg cgaaactggc ttcagagaaa aagaggatct tttatctcct ttgtccaatc
    69781 tgaatttccg caaaaaacaa gtgtatatat taattcttta ttatggggct ataaagatgc
    69841 aggattcaga gaaccagaag aaatttttag ggagagtggt ttgctgcatc tgttcagtct
    69901 atcgggtcta cacatccaat tttatttagg gtggctgtat tatttatttc gacgtatcgg
    69961 atggccacta cacgtcagta ttattccgtt gagtctacta actatctgtt atgtttctat
    70021 tgcaggaagt tctatttctg ttttgcgtgc tagtttgctg tttttattgc gattgctagt
    70081 caaattgctg aataagcatt attccaccat ggacttattt gcagttgtct tttggcttct
    70141 tttactattt gatcctcgat ttctttttca tgcgggtgga cagcttagtt ttttcatgag
    70201 ttttttgttt atcctgattt cttttgatgg agattggttg aaaaaaacgg cctctcacgt
    70261 cctgtttact atgatcacga tgccgttgat tgtatggcat ttttatgagt ggcctatttt
    70321 aggtagtctt tttacattac tgattgcacc ggtattcaag tttctttttc ttcctggtgt
    70381 gttatttctc acactcttca atcgctggct gccggaaact ttacttttgc tgatagaaga
    70441 gggattaatc ttatttgagc gaataatcga tttttcttca gggtcgtctc tcattatagg
    70501 acgtttgccc cttgtgctat ctgggatgta tattttcgga ttactgattt gtatggatag
    70561 gttgaaagaa caacctgtga aacacttgtt ttatgttggt cttttttctt ttattttttt
    70621 gactttgcca tttttacgct tttcttcaac gatcgctttt atcgatgtcg gtcaaggaga
    70681 cagtatcttt ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata ctggaggtca
    70741 gttacacttt gaaagagaaa agtgggcgca aggtatgata cgtcctcctg ctgaaagaaa
    70801 tatcgttcca tatctaaaag gtcaggggat aagtgtaatc gataaactca tcttgacaca
    70861 cgacgatacg gatcatgtgg gagaattacc cacgttatct cagcatttta ctatcaaaaa
    70921 aatttatatc ggttggggcg ctggacgaaa tgagccatta caaaggcatt tatctgaagc
    70981 tcaaaaaaat ggtactgaga tcattgaaat caaacatggg gacaggatca gcggttatta
    71041 tgatctttat gtattgactc cttttgaaaa gggagaggga agaaatgaag attcgattgg
    71101 tctatggatg gaatataaca ataggcgctt tttattttta ggagatttga atcaagcaat
    71161 ggaaaaacaa ttgcttttga tttatcctaa tctgaaagcc gatgtagtca aattaggtca
    71221 tcacggtagc cgtacgtcaa gtaacacaga ttttctttcc catattgaag caaaacatgg
    71281 gattatatcc tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag tattggagac
    71341 actagaagaa aatcagataa aaacatggcg aacggatcaa caaggaatga tcacctatcg
    71401 ctggcatcca gtttttcatc ctcatgggct agtggaaaca atgatagact agtaattctc
    71461 atctgctcat ggtaggataa tttttgaaag tggtgagtaa tttgagtata caagcagagt
    71521 tacaaaaaat acgtaaagaa aaacttgcat cttgttactt gattttagga acagaaaagt
    71581 ttctccaaga tcaagttcgg acagaaattg tcaaaaaaat ccaattagag ggcgaagatg
    71641 acttaaattt tttaagtttt gacatggaaa atgcaagttt agatgaggta gtagctgaag
    71701 cagagacgtt gccgtttttc ggagatcaac ggttagtatt cgtagagaat ccttattttt
    71761 taacaggtga aaaagtaaac aatggaatcg aacaaaatac tgatttattg acggcttatt
    71821 tgaaagaacc attagaatcg actgtattag tattctttgc gccatatgaa aagttggatg
    71881 aacggaaaaa agtaacgaaa caattgaaaa aaacagcaat tatcatcaat gtccaacagt
    71941 tgaatgaaaa agaagtacgt caatatctga tgaatacctt agagaattcc tcaatcaaaa
    72001 tggaacgaag tgcaattgat ttattcttga ggttgaccga cttagattta tccaaaatga
    72061 tgagagagtt acagaaattg gtcctttatg gacaaaatca acagcaaatc acggtaagag
    72121 aagtagaaga attagttcct aaaacgttag aacataatat ttttgacatg acccaatata
    72181 ttttaaaagg gaagacggaa caagctttgc gtttatacga agatttagtt atgcaagggg
    72241 aagaaacaat caaaattaat gcgattttgc tttctcaatt acgtttattt ttacagacaa
    72301 aatttcttgt aaagattggt tatcagcagg caaatattgc tgaaacattg aaaatccatc
    72361 catatcgtgt aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat ttgctggtac
    72421 agttgtttga tcaattagtt gagatggatt atcaaatcaa aacgggacaa atcgaaaaag
    72481 aattaagttt tcaactattc gttttgcgca cagggcagtt gttaagaaat cgataagaag
    72541 aaagacgagg gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt tgacaaataa
    72601 aaaaatcggc cgaagccgat tcttttattt agcgattttt ttgcttaaac gagatttgtc
    72661 gcggttagct ttgtttttgt ggattagacc ttttgattca gccatatcaa tagctttaac
    72721 agcttcttta tataacgcat ctacgttgtc agcacctgaa gctacagcat cttcaaattt
    72781 tttgattgca gtacgcatag cgcttgtttg agatgaattt ttcacattag cgttttcgct
    72841 tgtacgaaca cgtttgattg cagattcaat gtttggcatt agtttcacct ccgataactt
    72901 aagttcttac gcgtgttacc gcataattca acattcatta ttatacctaa aggcagcgtg
    72961 ctttgcaata tgaacatgac aagttttttt ccttaacagt cttttattag gatttttttt
    73021 cagtaaaaac ggatagtaca agctgaaaaa aatcaaaaag acgctattgt gggaggcaat
    73081 agcgtcaaac caaactagat ttttatttat ctatttaaac aaagtttgaa tgggaatgtt
    73141 tggtactaag ttattatata acaaagagaa ccgtttgcaa agtttttttt gaaaagggat
    73201 acgattccct gttgttcttt gtttttctat taagaaactg cagttatttt ataaccagta
    73261 tcctttgatc agtaaaggct attct
//
LOCUS       DACDIP010000009        66013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDIP010000009 DACDIP010000000
VERSION     DACDIP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.009"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            27..620
                     /locus_tag="IV257_000685"
     CDS             27..620
                     /locus_tag="IV257_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8813835.1"
                     /translation="MGTIVVVLGLLGLLFAPSIISIFSLTITGFIAFTLVFGKKDTKK
                     MFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIM
                     ILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGV
                     ARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(739..811)
                     /locus_tag="IV257_000686"
     tRNA            complement(739..811)
                     /locus_tag="IV257_000686"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:Thr,seq:cgt)
     gene            932..1174
                     /locus_tag="IV257_000687"
     CDS             932..1174
                     /locus_tag="IV257_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813836.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(1313..1942)
                     /gene="upp"
                     /locus_tag="IV257_000688"
     CDS             complement(1313..1942)
                     /gene="upp"
                     /locus_tag="IV257_000688"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8813837.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(2076..3023)
                     /locus_tag="IV257_000689"
     CDS             complement(2076..3023)
                     /locus_tag="IV257_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8813838.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(3176..4420)
                     /locus_tag="IV257_000690"
     CDS             complement(3176..4420)
                     /locus_tag="IV257_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8813839.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(4595..5620)
                     /locus_tag="IV257_000691"
     CDS             complement(4595..5620)
                     /locus_tag="IV257_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8813840.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(5642..5821)
                     /locus_tag="IV257_000692"
     CDS             complement(5642..5821)
                     /locus_tag="IV257_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813841.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(5814..6653)
                     /gene="prmC"
                     /locus_tag="IV257_000693"
     CDS             complement(5814..6653)
                     /gene="prmC"
                     /locus_tag="IV257_000693"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8813842.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(6646..7719)
                     /gene="prfA"
                     /locus_tag="IV257_000694"
     CDS             complement(6646..7719)
                     /gene="prfA"
                     /locus_tag="IV257_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8813843.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(7722..8306)
                     /locus_tag="IV257_000695"
     CDS             complement(7722..8306)
                     /locus_tag="IV257_000695"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8813844.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(8412..8881)
                     /locus_tag="IV257_000696"
                     /pseudo
     CDS             complement(8412..8881)
                     /locus_tag="IV257_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8987..9589
                     /locus_tag="IV257_000697"
     CDS             8987..9589
                     /locus_tag="IV257_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8813845.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(9693..10262)
                     /gene="lepB"
                     /locus_tag="IV257_000698"
     CDS             complement(9693..10262)
                     /gene="lepB"
                     /locus_tag="IV257_000698"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8813846.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10492..11259)
                     /locus_tag="IV257_000699"
     CDS             complement(10492..11259)
                     /locus_tag="IV257_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813847.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(11256..11735)
                     /locus_tag="IV257_000700"
     CDS             complement(11256..11735)
                     /locus_tag="IV257_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8813848.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(11860..12597)
                     /locus_tag="IV257_000701"
     CDS             complement(11860..12597)
                     /locus_tag="IV257_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8813849.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            12758..14158
                     /locus_tag="IV257_000702"
     CDS             12758..14158
                     /locus_tag="IV257_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8813850.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            14381..15733
                     /locus_tag="IV257_000703"
     CDS             14381..15733
                     /locus_tag="IV257_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8813851.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            15726..16424
                     /locus_tag="IV257_000704"
     CDS             15726..16424
                     /locus_tag="IV257_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8813852.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(16486..17637)
                     /locus_tag="IV257_000705"
     CDS             complement(16486..17637)
                     /locus_tag="IV257_000705"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8813853.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(17650..18087)
                     /locus_tag="IV257_000706"
     CDS             complement(17650..18087)
                     /locus_tag="IV257_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8813854.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(18102..19868)
                     /locus_tag="IV257_000707"
     CDS             complement(18102..19868)
                     /locus_tag="IV257_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8813855.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNGMVNPQKYESIAQYLKQTA"
     gene            20132..20809
                     /locus_tag="IV257_000708"
     CDS             20132..20809
                     /locus_tag="IV257_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813856.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            20812..21939
                     /locus_tag="IV257_000709"
     CDS             20812..21939
                     /locus_tag="IV257_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8813857.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(22139..22579)
                     /locus_tag="IV257_000710"
     CDS             complement(22139..22579)
                     /locus_tag="IV257_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8813858.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(22590..24623)
                     /locus_tag="IV257_000711"
     CDS             complement(22590..24623)
                     /locus_tag="IV257_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8813859.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(24625..26328)
                     /locus_tag="IV257_000712"
     CDS             complement(24625..26328)
                     /locus_tag="IV257_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8813860.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(26422..27372)
                     /gene="manA"
                     /locus_tag="IV257_000713"
     CDS             complement(26422..27372)
                     /gene="manA"
                     /locus_tag="IV257_000713"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8813861.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(27411..28436)
                     /locus_tag="IV257_000714"
     CDS             complement(27411..28436)
                     /locus_tag="IV257_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8813862.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(28645..29514)
                     /locus_tag="IV257_000715"
     CDS             complement(28645..29514)
                     /locus_tag="IV257_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8813863.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(30218..32110)
                     /locus_tag="IV257_000716"
     CDS             complement(30218..32110)
                     /locus_tag="IV257_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813864.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(32107..33834)
                     /locus_tag="IV257_000717"
     CDS             complement(32107..33834)
                     /locus_tag="IV257_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8813865.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(33850..34500)
                     /locus_tag="IV257_000718"
     CDS             complement(33850..34500)
                     /locus_tag="IV257_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8813866.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            34704..35000
                     /locus_tag="IV257_000719"
     CDS             34704..35000
                     /locus_tag="IV257_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8813867.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            35192..35812
                     /locus_tag="IV257_000720"
     CDS             35192..35812
                     /locus_tag="IV257_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8813868.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(35904..37070)
                     /locus_tag="IV257_000721"
     CDS             complement(35904..37070)
                     /locus_tag="IV257_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8813869.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(37082..37435)
                     /locus_tag="IV257_000722"
     CDS             complement(37082..37435)
                     /locus_tag="IV257_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8813870.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            37563..38585
                     /locus_tag="IV257_000723"
     CDS             37563..38585
                     /locus_tag="IV257_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8813871.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(39024..39458)
                     /locus_tag="IV257_000724"
     CDS             complement(39024..39458)
                     /locus_tag="IV257_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813872.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(39568..41427)
                     /locus_tag="IV257_000725"
     CDS             complement(39568..41427)
                     /locus_tag="IV257_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8813873.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(41545..42705)
                     /locus_tag="IV257_000726"
     CDS             complement(41545..42705)
                     /locus_tag="IV257_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8813874.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(43012..44436)
                     /gene="araA"
                     /locus_tag="IV257_000727"
     CDS             complement(43012..44436)
                     /gene="araA"
                     /locus_tag="IV257_000727"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8813875.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(44452..45150)
                     /locus_tag="IV257_000728"
     CDS             complement(44452..45150)
                     /locus_tag="IV257_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8813876.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(45107..46768)
                     /locus_tag="IV257_000729"
     CDS             complement(45107..46768)
                     /locus_tag="IV257_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8813877.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            46986..48386
                     /locus_tag="IV257_000730"
     CDS             46986..48386
                     /locus_tag="IV257_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8813878.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(48638..49717)
                     /locus_tag="IV257_000731"
     CDS             complement(48638..49717)
                     /locus_tag="IV257_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8813879.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(49803..50762)
                     /locus_tag="IV257_000732"
     CDS             complement(49803..50762)
                     /locus_tag="IV257_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8813880.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(50774..52297)
                     /locus_tag="IV257_000733"
     CDS             complement(50774..52297)
                     /locus_tag="IV257_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8813881.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(52307..52870)
                     /locus_tag="IV257_000734"
     CDS             complement(52307..52870)
                     /locus_tag="IV257_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8813882.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(52876..53739)
                     /locus_tag="IV257_000735"
     CDS             complement(52876..53739)
                     /locus_tag="IV257_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8813883.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(53753..54649)
                     /locus_tag="IV257_000736"
     CDS             complement(53753..54649)
                     /locus_tag="IV257_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8813884.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(54824..56095)
                     /locus_tag="IV257_000737"
     CDS             complement(54824..56095)
                     /locus_tag="IV257_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8813885.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(56085..57602)
                     /locus_tag="IV257_000738"
     CDS             complement(56085..57602)
                     /locus_tag="IV257_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8813886.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            57800..58690
                     /locus_tag="IV257_000739"
     CDS             57800..58690
                     /locus_tag="IV257_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8813887.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(58763..59587)
                     /gene="nadE"
                     /locus_tag="IV257_000740"
     CDS             complement(58763..59587)
                     /gene="nadE"
                     /locus_tag="IV257_000740"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8813888.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(59599..61071)
                     /locus_tag="IV257_000741"
     CDS             complement(59599..61071)
                     /locus_tag="IV257_000741"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8813889.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(61708..62070)
                     /locus_tag="IV257_000742"
     CDS             complement(61708..62070)
                     /locus_tag="IV257_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8813890.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            62293..63045
                     /locus_tag="IV257_000743"
     CDS             62293..63045
                     /locus_tag="IV257_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8813891.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(63185..64108)
                     /locus_tag="IV257_000744"
     CDS             complement(63185..64108)
                     /locus_tag="IV257_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8813892.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(64123..64395)
                     /locus_tag="IV257_000745"
     CDS             complement(64123..64395)
                     /locus_tag="IV257_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813893.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(64739..65926)
                     /locus_tag="IV257_000746"
     CDS             complement(64739..65926)
                     /locus_tag="IV257_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8813894.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT        19018 a        13611 c        11519 g        21865 t
ORIGIN      
        1 aaaaaataga acttaaaaaa gggatattgg gcactattgt cgttgtactc ggtttgttag
       61 ggttgctatt tgcgccatcc attattagca ttttttcttt aactatcact ggatttatag
      121 catttacact tgtttttggt aaaaaagata cgaaaaaaat gttctcgaaa cctgtcgctc
      181 ctgtcaaaaa tatagccaaa tattttttta tcaatgtagt aatcagtgcc gcagtctcgg
      241 tatttttgca acaaattttg aaatggggtt taacaggcaa tccaataaat gaggctttca
      301 gcccgttatt atttattatt ttgcctatca tgatacttgg agaagaattg ttctcagtgt
      361 attttctcgc tattttttct agtaagttca gcattcctgt cgctagtttc ttgtcagcaa
      421 tcatctttgg atttatccat tacagtactt atgataatgg aaatatttta cacactgtag
      481 cacacatcct tttgatacaa ggggtggcaa gattgttatt caaccaagct gccattaaaa
      541 gcaactcgat cattacaagt tgggcagttc atgtgatatt tgatttcaca gctatattgc
      601 tagtcgtttt attctcctaa atttttatag aaataataca tgctctattg ctaagaaagc
      661 atactttatt tcctttaaac aaaaagacag atgtatcaat ggattaaaaa tccttgatat
      721 atctgtcttc tcttttaatg ccagctatag ggatcgaacc tacgacctac gcgttacgag
      781 tgcgttgctc taccaactga gctaagctgg ccttatccaa aagaaattat atactctatt
      841 tggctgattg taaagagtcc gatggaaaat tagtttaatt tcatgatatt tttcataaaa
      901 cgtggtataa ttgatgtgaa aggcaggttt tatgaaaatg gcaacttttg gaaaatttga
      961 tacaactatc gtaccatcgg aaattggtga aaaattcaag atcaatactc acgtaatgtt
     1021 cacaattcac ggcaagtcgc aaactggaac gatttcaaag caattgaaaa attccgctgt
     1081 tgttgagatt gatgaaactg cagaaaatgc gaacttgatg acgcaaagta agggtgtagt
     1141 aatcgttaac tacaaagaac taaaaaagat ttgaatttga ccatctctgc atttttggga
     1201 tggttttttt atatgcaaac gaaagctgtg aaaaagctgt tctgcatcaa acgttaaaaa
     1261 tgaccaaaaa aggatgtgac aaaagttgtg tcacaccctt tttcgtattt acttattttg
     1321 taccaaacaa acggtcaccc gcatccccta gtccaggaac gatataacca tgttcattca
     1381 atttttcatc tagagaagct gtatagatgt caatatcagg atgcgcttct tgcaaagctt
     1441 ttactccctc aggtgctgct accaaacaaa caaatttaat gttcgacgcg ccacgttctt
     1501 tcaatgcatc gattgccatg attgctgatc cgcctgttgc caacattgga tctactacaa
     1561 acaactgacg tgcatcaatg tcttctggaa gtttgacgaa atattcatgt ggttccaatg
     1621 tttcttcatc tcgataaaga ccgatatgtc ccactttagc agctggaatc aattccaaga
     1681 taccgtcaac cattccaata cctgcgcgca agattggaat aatcgctact tttttaccag
     1741 acaacgtttt ttgtgttgtt tcagtgatcg gtgtttcgat cacgatgtct tccaatggca
     1801 tatcccgtga tacttcatat gccatcaaca tagcgatttc atcgactact tcacgaaaaa
     1861 cctttgttcc gcagtttttg tcccgaataa tagttagttt gtgttgaatc aatggatgat
     1921 cgatcacttg aaatttgccc attttttaag ctctcctttg ttctgtcgtt tctttttaat
     1981 tgtaaaacaa aatcaagtga ttgactagtc aaactcgcca tttttcctaa gaaaatccgt
     2041 ttttgaaaag aagttttcct gcggacaagc aaagcttatt cttttttctt gatccaagaa
     2101 gtagataatg ccattgctgc acgcgttgcc tttgtctgat ccatagcatt taccatgaca
     2161 aaatcatgga tgattccttg gaagcgtact tgtgtaacag gaacccctgc atcacgaagt
     2221 ttgcgcgcat aagcctcacc ttcatctctt aatacatccg cttctcccgt taaaatcatt
     2281 gctgaaggaa gatcatttag ttcatttata tttgctctca aaggcgaagc tgtgatttca
     2341 gcacgctctt cttcattcgt cgtatattgg tcccaaaacc acatcattcc ttctcttgtc
     2401 aaaaaatagt tttctccgaa ttcatgatag gaatctgtat caaaatcagc gtttgtgact
     2461 ggataataca gcaattgctt ggatattggg aggccctttc gttcttttgt taagatcgtc
     2521 atgactgtag ccatgttacc acctactgaa tctcctgcaa cgattagatt ctcgagatcc
     2581 agattttttg acttggctaa tttattcagc tgttgcaaca cagcataatt ttgttcaatt
     2641 gctgttggat attttgcttc tggtgatcga ctatattctg gaaagacaac gacggaattc
     2701 gtacgaaccg caagttctct gatcaattta tcatgggttt gagcactgcc aaaaacccag
     2761 cctgctccat gaatataaaa aataactggt aatttatcca tattcccttc tggtcgtacg
     2821 agacgaatat tgagttttcc ccattcgcca gtatccacca tcaagtcttc aatatcgacg
     2881 gaatatttgt tcacaggtga ttcttgtact tcttctagta aaactcttcc ttcttccggt
     2941 gctagttcat agatacgcgg atgaggcgca tttgcatcac tgaatgctac tgcttccttt
     3001 tctaacgaga tacgatcagt catgactttt cctccctgag atttagtgtg aagactaaaa
     3061 ttacacctca tatactagag agcatcaccg atattgtcaa agaatccgtt ttcccaaaaa
     3121 ataaacagaa aagcccaaca ttgaactttt ctgtttacta atggaattta aatttttatt
     3181 ttttatgcaa cggaaatttt tcagttaatt cgcgtacacc tgttttgact tcgtctagtt
     3241 gagcgttatc atcttttgct tgtaatgctt tgacaactag ttcagccact tttacagcat
     3301 cttcttcttt gaacccgcga gtagtgattg ctggcgtgcc gattcgaata ccgcttgttt
     3361 tgaatggact tagtgtttca aaaggaatcg agtttttatt tacagtgata ttcacactgt
     3421 ccaaaatgct ctctgcttct tttccattta tacctaactc gcgaacatcg atcaacatca
     3481 gatgattatc tgtcgcccct gaaatgactc tggttccaat tgcttgattg aaaactttga
     3541 ccattgcttt tgcattggcg atgatttgtt cactatattc tttaaatgca ggatctaatg
     3601 cttctttgaa tgctactgct ttacccgcaa tcacatgctc tagagggccg ccttgaatac
     3661 caggaaatac cgcactattt attttttttg ctaatgcttc atcgtttgtc aaaatcatgc
     3721 cgcctcgagg gccacgcaat gttttatgag tggtagtcgt tgtgatatct gcataaggaa
     3781 ctggattagg atgaagtcct gctgctacta atcctgcgat atgtgccata tctaccatca
     3841 gttttgcgcc tacttcatct gcgatttctc tgaatttcgc aaagtcgatc gttcgtccgt
     3901 aagcactggc acctgctacg atcaattttg gttgatgttt tctagctaaa atacgtacca
     3961 cattataatc aataacttct gttgtcggat caacaccgta agcaacaaag tgataggttt
     4021 tgccactgaa gttgactggt gatccatgtg ttagatgtcc gcctgcggaa agatccattc
     4081 ctaagatcgt gtcccctggt tctaccaaag cgagataagc agccgtgttg gcttgagagc
     4141 cggagtgagg ctggacgttc gcaaatttcg ctccgaataa ttcttttgca cggtcaatcg
     4201 ctaaattttc aacgatatcg acaaattcac acccaccata atagcgatgc cctggataac
     4261 cttctgcgta tttatttgtc aagatacttc cttgtgcagc catcactgct tcggagacaa
     4321 agttttcaga ggcaatcagc tccaaattat gttcttgtct ttcttcttct ttggcgatcg
     4381 cagcccaaag atcagggtca aacgttttgt aatctaccac aaacaacagc tcctttttat
     4441 tttcctacaa ttttattttt cccatcaaaa acaaattgcc aaatattcgt atctcttcta
     4501 ttacgattag ttacgcttag tttttcgttc actgatagaa gatttacttg atagcattgt
     4561 atcatactaa aagaatgcga caataaccaa ttgatcattt ttcaaaataa ttttgtcctg
     4621 ctgctttttt caagcgattc atgtaagcat tccccaatcc agtttctgga tacacttgta
     4681 caaaaatcaa atctaatcca agtgttggtt catctaatcc tcttagacct gcaaacaatc
     4741 ctttagccgc cgcttctact gagttgtcgt tatacatata aacagctgca gtatctgtcc
     4801 gcacttgatc cgcaatttcg gggcttgcta tgacacctgc acggatcttc ttattttttg
     4861 cccattgaac tgcagttgac caatcgccat ctctgaccat cagaacacgt gtatcgggtg
     4921 agtagtgttt gtatttcatc ccaggggctt ttggtgtttc attttctttt acaagatgct
     4981 gatcaatagc aacaggagat tcgatgactg cttcgatttg ttctttcgtt actgcacctg
     5041 gtcgtaaaat cataggcatg gctgttggat cacttaaatc aagaacagtg gattctacac
     5101 caattcgcgt agctccatca tcaataatcc cggtaatttt tccttgtaga tcatgatata
     5161 catgatctgc agtcgttgga cttggttttc ccgacgtatt tgcacttgga ccgacaagcg
     5221 gaacaccgga aagttcgatt acttctagtg tttttttgtt atccggtata cgaaatgcag
     5281 ctgtggataa tcctcccgtc acaactgagg gcaaagtgtc ttttttgatc ttaaatatca
     5341 aagttaatgg tccaggccag aagttcttca caatcttctc tgctgccgga tgaaagttgt
     5401 ccacatactc ttttacttgt tcaaaagagg cgacatggac gattaatgga ttatcttgcg
     5461 gtctcccttt tacagaaaac actttcttga cagcgtttgg taaaagagcg ttggcgccca
     5521 atccgtatac tgtttcggta ggaaaagcaa caagttctcc ttctttcagt gcttcggctg
     5581 cctcttgcaa ttctgtttca tcatatcgtt tcgtttccac agtttatcca caaccccttt
     5641 ttcatacatt cttattttgt ctgagtttat cctcaagttt tccactgttt gtggataatc
     5701 tatttgtcaa tagtggataa acttgttgat aaaccaaaaa gtcgtttaca tccttgtcaa
     5761 ataagtgttt ttttataata aaaaaacaaa taaaaaattt ttcatccaca acttcaacca
     5821 taaacttgga tcattcgttc attgccaaaa agatcctttt ttatcgccac ttttttatct
     5881 ggaaatgctt gctgaaatat tttttttact gcttcacctt ggcgaaatcc gatttctaaa
     5941 aaaatctttc catttgattt tagtacgctt ggtgcttcaa ctgctatttt ttcataaata
     6001 gctaatccat cgttctcagc aaaaagggcc attttaggtt cgaatgaccg gacactttca
     6061 tccatcagtt cccattcatt ccgactgatg tacggcggat tagaaataat cacatcaaat
     6121 tgctgatcca ttactggtcc taatgtatct ccatgataaa aactgatttt cgttccttct
     6181 ttctgagcat tttctttcgc tacttctaat gcttcttctg aaagatccac agcagatata
     6241 tgccaatttt ttcttgctgc tttcaaacta atagcaattg ctccagtccc tgtcccaata
     6301 tctatcactt ctaacggtac accaggcgtc tcgtctaaac accattcgac taactcctct
     6361 gtttctggtc gagggatcag tgttgcttca gttaccttta accgatgatc ataaaaatct
     6421 gcatatccta gcaaatactg aggtggatga tctgcaagca gaagtcgcag atcggtttcg
     6481 atcaaccgct gttcttcttc tgaaatctct tcgttcatat gaaggagcca atcaagtttt
     6541 ttccattgtt ttcgttccaa aaataaaaat tggatactat atgcttcttt cccatgatcc
     6601 tctaaaaaag aagaagcccg tgacaggact tctcgatagg tcattttacc cattttgcat
     6661 agcttccagt tttgatgttt gatcatacat gatcaatgca tcaacgatct catctaattt
     6721 ccctgctaaa atctgatcta atttttggat agttaatccg atacgatgat cagtcacacg
     6781 attttgcggg aaattatacg tccggatccg ttctgaacga tccccagttc cgactgctga
     6841 ttttcgattg gcatcatact cgctttgtgc ttcttgctgg atcttatcgt acacacgagc
     6901 acgcaagatt ttcattgctt tttcacggtt cttgatttga gaacgttcat cttgcatagc
     6961 aacaacgatc cctgttggta agtgagtcaa acgtacagca gatgctgttt tattgacgtg
     7021 ctgtccgcca gcacctgaag catgatagat atctgtacgg atatctttgt cttcaatatc
     7081 gatctctact tcttcagctt ctggcatcac gacaactgtt gcggtggaag tatggatacg
     7141 cccttgcgat tcagttgaag gaacacgctg gacgcggtgc gcaccacttt catattttaa
     7201 tttcgaatat acgttgtctc cggtgatcat catgatcact tctttatatc caccgattcc
     7261 ggtaacgtta gcttccatca cttctgtttt ccagccttgt gcttcagcat acttttggta
     7321 catattgaaa agatcgccag cgaataatgc tgcttcgtct ccgcctgctg ctccacggat
     7381 ttccatgatg atatttttat cgtcatttgg atcttttggt aaaagcaaga tcttgatttt
     7441 ttcttctaag acttcttttt ctttttttaa ttcagataat tcttcttttg ctagttcagc
     7501 catttcatca tccagttttt caccaagcag ttcttctgta tcggtgattc cttgtactac
     7561 ttctttataa cgacgataaa cttcgactgt ttcgcgcgtg tttgcttctt cttttgaaag
     7621 ctgcataaaa cgctttgtat cagaaatcac ttctgggtca cttaaaagtt ctccaagctc
     7681 ttcataacgg tcttcaattg cttgtaattg atcgtacatg cttattctcc ttctgtaatc
     7741 attgatgggt gaaagtaatg ttttcgacaa acggggtaat aagcttcatt tccgccaatt
     7801 tgcacttgat cgccttcata aacagggttt ccatcaatat aatgtaaatt catgattgct
     7861 tttttgtggc agaaccaaca gattgttttc atttcttcga ttttgtctgc ataaagcaat
     7921 aaatattttg atccttcaaa taactcgttt cgaaaatcat ttttcaaacc aaatgccata
     7981 accggaatat ccaattcgtc cacaattctc gtaaaagcaa tgacatgttt cttttttaag
     8041 aattgcgctt catcgatcag cacacaatag ggtttgcttt ccattttctg aattacgtca
     8101 aatacgtctg tttcttcaaa tatcggcatc gcattacgtt tcagtccgat tcggcttgaa
     8161 acaacgccta aaccatctct tgtatcaata ccgcttgtca ttaagacaac tggcttattt
     8221 tgttcttcat aattatgtgc gacttttagg atctcgatcg tcttgccgct attcattgcg
     8281 ccatacttaa aaaatagttg tgccatgctc tttttcacct tccagtttgt tcctatggta
     8341 ttatacttga ttttcagcat tttttacagt gagaattttc atcaagcaca gattagtatt
     8401 aaatctattg ctcacttttg ttccaatttt ttgacaagaa ctaaacatgg gttccattca
     8461 tcccataaat ttggaaaaac ttccaacctt gaaaatccta atgattcgta gaaacaaata
     8521 gtttgatcat atatagagta gtgcccctcg tctacagtct tcacttgcaa atatttgtaa
     8581 tgttttgctg cgtaaccttc tgcttcttta aaaagttttg ttcctaatcc ttggtgatga
     8641 tagtcttttt ttattcccat acaatagaat agatttcggc agtatcttca cttgtctgtg
     8701 aaagagtgat aaagcctaaa tattcttctt ttcttttttc acaccataaa ggtagtttac
     8761 ttgattcctc gatatatttt tccgttgatt caggaagccc aaaccactca ggcaagtcta
     8821 gtaatatttc tcggacacac tttccttttt cctctctatc ttttacaaac gatatcggca
     8881 tattctttct agattctccc tttttgaatt gagtatagca tgctttcttt cttttcacgt
     8941 gctataataa gttaaaattt caaagtttta cgtaaggagc tatttgatga caaaaaatat
     9001 ttttgattcc atcgctaacc attatgattc ccctgaccgt caagcattag ccaagattat
     9061 tcgcgaagaa ctaactactt atctaccgcg cgacagccac caaaaagtcc tgctggatta
     9121 cggaggcgga actgggcttg ttagtttacc gcttgcggaa agattcaaag aactgatcat
     9181 tgccgatgct tcagaaacta tgctgaaaat ggcagaagaa aagatccaag cagcagatct
     9241 gaaaaatgtc cgaacgatcc acgctgatgc ttctgtcgaa tttcctgctg tccaagctaa
     9301 tctcatcctt ctatcacttg tacttttaca cattcctgat acggagaata ttcttacaaa
     9361 actttatgaa attttagctc ctggcggtca attgatcatc gtagactttg acaagaatga
     9421 acaaattagt catccgaaag tccataatgg ctttagccaa gaagaattga ataatcgatt
     9481 ggaaaaaacg ggctttgtct ctgctactag tcataccttc catcgtgggg aaaagctatt
     9541 tatgaacaaa aacgcttcgc ttttcttatc catcagccaa aaagcttaaa atgagattgc
     9601 aaaaaaagtg agcaactcca agaaataaga aggaattttc ggaaaaagaa gctgtgacaa
     9661 aaaacatgtc acagcttctt tttatttttt catcacatga ttttaatttc tgaaagaggc
     9721 caataacgga aaacaacttt tccttggatc atatctttgt cgactaaccc aaacattcgg
     9781 ctgtcttttg aaatcaagcg gttatcgcct agaacaagat actttccttt tggcacagta
     9841 tattcaaagt cactcgtaaa ggtggatgaa ctttctgctt gtgcttggaa tcctgagcta
     9901 taagcatatt ctccttgtaa cttatcttct gaaaattctt ttttgaactc atccagataa
     9961 ggttcatcat actttttgcc attaatggtc aactggtcat tttccatctt gactgtatct
    10021 ccaggcatac cgatgatccg tttaacaatc atccgttctt catcccccgg ttcctttgta
    10081 gtaataatat ccattcgttc atagtttgag atcttagatg aaatcaatcg ttgtccatca
    10141 cttaatgtag ggtccatcga atgcccgttt acagtaactg gtgtaaacac gtacactcta
    10201 gctaataaaa tagccgctaa tacaaggatc aaaatccccc aatttttcaa aaattctttc
    10261 acagcttacc tcctacttgt attttcctcg ttatttatta cgttttttca caatacttcg
    10321 ttgtactttc tttattctac aatgtttata aatgaaagga aagaataagt gatgtgttcg
    10381 tcttatcttt ctcgttatat aaaaaaggaa cgtgacaaaa gacgtgtcac atccccctta
    10441 ttccgaataa acgtttttat cgcaaccact gatacacggc gtttcatcac attatgaatg
    10501 tcttcttctt tggctacgac tataaaccac tggttcttcc tgcgactgtg ccttttttgc
    10561 aatcaatttc agacgataag gctcatgata gcgtaacccg tctttgacaa aatcaccgcc
    10621 gacgatgaac aatccgacag gttccaatgt gtcttctgtt gagtcaagtc gaatggtccc
    10681 tactttttcc atgtccgagt agcttcgtcc aatatttaga acatactctt tctctcctgt
    10741 tttcttgaca tagtaataag gatacaatgt atttctatcg accagaaatc catccatgcg
    10801 accgagttca tttgttttga tctcaccatt tccaatcaaa taccgcatat tctcggctaa
    10861 tttcaattga taatcttcgt cataagaaac catgacctct tgtgtattca ctttatcgat
    10921 cgaatttcca ttatctatcg attcttctgt ctggatgttg aacgcttcat caggtaaata
    10981 cttcacaaaa agctgagtga catcggcttt cgtgttttta tctaaaaagt aataaaatac
    11041 atttaaaaca gtaaaataaa caagcactag aaatgcaagt ccatataaga aaaattgaag
    11101 atagttttgg ttttgccaaa gtccataaac aattctcaat aaataaagcg tgggaatcac
    11161 acttaaaatc gtatatccac ggttaagata tttaggactg atgtctaaat agccaaggaa
    11221 tttatgcacc ccgttgataa ggtctagtaa aaatgtcatt gagaatcacc tgcttgctgg
    11281 ttatacgttt gggtatttcc tgaatcttgg ttttgctgtg ctgtacgtgc tgcatcatca
    11341 ttttgcgtgt tatcatctac agtcgtactg cttggtatcg tttcggagct agcacttgta
    11401 tccggacttg tacttggatt ggttgaatcc gatggagtgc catctggtac cgtattactt
    11461 gtttcgcttg agctgtcagt tgtatatgtc tcatcatacg acgaactagt tgttgtatcg
    11521 tcccatgtac ttgtcgtagt ggtttcattc aaactttccg ttgttgttgg ttcagatgtc
    11581 gagactgaag tagttggtgt tgtttcttct acagtactag aacttgaact tggtgcacgg
    11641 tagctggaac tattcacaat acctgtagta gtcgcgacaa tcgtcgagat tgttaataca
    11701 gcgattggct tcaaaatagg tgctactttt cgcatcttat ctcttccttc attgaaattt
    11761 attcttgttt ccttacttta ctacggacaa attatacagt ctaccttatt tctaccataa
    11821 attctttatt tttttcttac gtttttccga aagatttctt taattttcct caaaaactac
    11881 ggttgatgtt tttaagtaag cttttgcttc ccgattgatt tctttcagat cgcatccttg
    11941 ctgcttggca gcgaacacgc aaccacaata acattggcga taaatatcat actctttgca
    12001 catttgcaaa gagcgttcat agcctttgtt tttcttgaaa tcacttggca agtattgtgt
    12061 cccgtaaatc ttttggatat ccattccgat ctgattgatc aaagctgcat ttttctttgg
    12121 cgagatggtc agggcacttc caaagtaatc gtatcctagt tcttgcgctt tttgcgcaac
    12181 aagatctaat cgcatattga agcatgcact gcagcgagca ccaccttctt tttcgttttc
    12241 aagatggttt gttaaaacca ttttggtgaa ttcatttgga cggtattcat caacgattag
    12301 cgcaacatga tggtcagttt tctcattaaa ttggtctaca aatcgttttt gttcatgtaa
    12361 tcttcgcaaa tactcacttt taggatgaat attcgaattt gaaaaaaata acgtgatctc
    12421 tgcatattga gacatgaatt ccaatgtgtg cgtactgcaa ggagcacagc atgtatgtat
    12481 taatatctta ggctttactt cttctttctc ccattgcatg atcatttttt tcagaactgt
    12541 atcataattt attttttgat ttttcatttt ttctacgatt tgttgagcat taatcaattt
    12601 gttattcctc ctccttcttt tttctaggat tttccttatt ataagccatt tctattttaa
    12661 tcgaaagctg tattgttatc tgtttgataa tttctttgag tattcctaat tccccttgta
    12721 tactggtaga aacgactatt gtcaggagga tagtactgtg catgttgatt tattctttcg
    12781 aaggctgcag cggcgaggac agaagtttgc tgccaatcat ttctcatcga tccaaatcat
    12841 cgttttttat tatatattga tgacggtctt atcactcgtt ctattttata tgccgatttt
    12901 tcgcgaacca gatagccatg tttcttttgt ggacatgctg ttcatggcaa tcagtacagt
    12961 gagtgtgact ggactatcga cttttgatat caattctgtg ttcaatgata acggtattat
    13021 actgctggaa atattatttc aagttggtgg tttggggatc atgatgattt ctactgcttt
    13081 tgtcattttt tctaaacgac ggatcacact gaaacagcga cagctgatca tgacggatat
    13141 gaatcaaccg cgtttgtctg gtatcgtccg actgattcgg atcacttttg ctattttaat
    13201 ctggtttcaa ttactttttg ggacattctt ttctatttat ttttattaca gaggctattt
    13261 tgatcgttgg cgtgatgccg ttttttacgg tttttatcag gcgatatcgg ctgtcacgaa
    13321 ctctgggttt gacgtcactg gggattccat caagcccttt gcacatgact attttttctt
    13381 gatcctgatc atgtttttga tttttgtcgg aggaattggt tttcctgtat tgatggagtg
    13441 ccgggaatgg ctattataca aacgttccaa tgcgaagctt ccgtttcgtt tttccttgtt
    13501 taccaaatta gctgtgttag cttttgttat cttgtttgtt tcgggaactg tcttgattta
    13561 tctattggaa aaggaccatt tattccaaga ttccaatatg agtgtcaaat ggatcaattc
    13621 gatgttttat tcgattacga ctcgtaatgc tggtctgcag attcacgatt tgggtgattt
    13681 tcaaatcaca acactgatca ttttttcttt actgatgttt atcggatgca gtcctagttc
    13741 tgtaggcggc gggattcgaa caactactgt agcaatcatc ggtttgtatc tatattcctt
    13801 cctcaaaagt gaagataaca tcaatatttt tggacggaga atcgatcaag atgatgtgcg
    13861 aaaatcagtc gttgtcttta tgctttctct tggtatgtgt ttcttttgta tcgttttttt
    13921 atctgctaca gaagaacaaa cgctgatctc gatcatcatc gaagtaactt ctgcgtttgg
    13981 aacaactggt ttatctttag ggattaccgg agacttgtct gttgtgggca aaatcacgat
    14041 tgctacactg atgtttattg ggcggatcgg aatgctatat acattgatgt tattcgtgcc
    14101 aaaagaaaca cgagatctag gctatgaata tccaagcgag aaaattatta tcggttaaaa
    14161 tgggaatata ggtaagaggg aataataaaa tcacagcagc ttgaaacaat cattttgttt
    14221 caagctgttt ttttgttaaa tcatgcgatt ttgtccgatt tcatgcactt tcagccgtaa
    14281 ccttttctta agaataagaa aagcaattta accttttttt caacatatgg tatgattagt
    14341 tgaatttatt cgtcagcaaa tcgtttttgg aggttacata atgggaatac gcagtcattt
    14401 tgcgattgca gttggaaaaa cgtcgcaatg ggcattaaaa acttttttta aaggcggatc
    14461 aagtttacca ggtaaactag cgctgaaagt cgatccacat atactagata gtttggcaaa
    14521 agactaccaa gtcgtcgttg tcaccggaac aaacggtaaa acattgacta ccgctttaac
    14581 agtcaatatt ttgcgccagc aattcgatga agttctaacc aaccctactg gtgcgaatat
    14641 ggtacaggga attgtatcga ctttcttgca ggcaaaacca aaaaaaggac agaaaaagtt
    14701 tgctgtccta gaaatagatg aagcaagttt gagcaaagtc acagaatata tgaaaccaga
    14761 actgttctta tttacaaata ttttccgtga tcaaatggat cggtatggag aaatctatac
    14821 gacctataaa atgattgtag acggtgccgc aaagtcacca aatgcaacga ttatcagtaa
    14881 cggtgattca ccaatcttca attctatcga aacagtcaat cctagaaaat attatggctt
    14941 tgaccatgaa gaagatcgtg aacaaatggc tcattacaat acagacggtg tcttatgtcc
    15001 aaattgtcat cacatcttgc attataaaat gatcacttat gcaaacttag ggaaatatta
    15061 ctgtccaaat tgtgacttca aacgtcctga attggattat cgattaactg atatggtacg
    15121 gatggacaat gtgtcagcag atttcgtgat cgatggtcat gaatacggaa tcgaagttgg
    15181 cggtatgtat aatgtctaca atgctttagc agctactgca gtggcagagt acttcaatgt
    15241 cgatcctgat aaaatcaaac aaggtttagg atatgacgag aaagtattcg gccgacaaga
    15301 agtcatcaat atcaatggga aaaaatgtac attagtactt gtcaaaaatc ctgtaggtat
    15361 caatcaagta atcgatatga ttggtctagc tccttatcca ttttctttag tggctttatt
    15421 aaatgccaat tacgcagacg gaatcgatgt cagctggatt tgggatggta attttgaaaa
    15481 ctttgccgat atggatatcc cagctgttat ttctggagga gaccgtcata cagatatgac
    15541 tttacgtcta aaagttgccg ggattccaga aaataaattg acagaaacaa aagaattacc
    15601 agaagtaatc gaaaaaatca aagaattgcc aactgagcat gtctatattt tagctactta
    15661 cactgctgta ttgcagcttc gtaaagaact agctgctcaa ggatttatca aaggaggaat
    15721 gaaccgtggc taattatgaa ctcaatatcg cccaccttta tggaaatttg atgaacacat
    15781 atggtgataa cggaaacctt ttgatgttga aatatatagc agaaaaaatg ggggtttctt
    15841 gccatacgga gatcgttagt atacatgaac cttttgatgc tgataaatat gacttggttt
    15901 tctttggcgg tggtcaagac tttgagcaat taatcatttc aaaagacatc cagaaaaaga
    15961 aagattcttt gatcaactat atcgaaaatg atggcgtcat gttagccatt tgtggtggct
    16021 accaattgct aggtcactac tacatgggag ctaatggaga aaagatccaa ggaatcagtg
    16081 cactggatca ctacacgtta agtcaggaaa ataaccggtt tatcggtgat atcgttatcc
    16141 ataacgaaga attcaatgaa acttattatg gatttgaaaa ccacaatggt cggactttct
    16201 taggtgaagg tgaacgacct ttggggaaag tggtacaagg tcaaggaaat aatggtgaag
    16261 accaatctga aggtgtcatt taccgaaatg tcttcggttc ttatttccat ggacctatcc
    16321 ttgcaagaaa tgagaacctg gcagctcgtt tgatccgcct agctttagaa caacgttacg
    16381 gtaaagaatt ggatctgccc tatcttgaag gacaaacagc ctagttattc gaaaaagggg
    16441 aatgtgacaa aaatttcgtc acattcccct ttttcgtatc ctattttaag cgacttgctt
    16501 ggcataacgg ttcacgtttt gcttgatatt cccgattgtt ttcggatcat tgattcctgt
    16561 gacttcttga atcaattgtt ccaagttttc atggatcttc atttcatgca attgacggct
    16621 ttgttcatct tcaggatcat agtaattaaa aataatcccc attgttgccg tcaaatgtgg
    16681 gcaagtcaag ttatgttctt gtagttctct cactggacgt gtaaatcttt cttgataacc
    16741 taacttacgt aatggtgtcc gagcgacacg tgtgatcgca tcagagatat ttttgttttg
    16801 gaagcgatga atgatttttt caatatacgc atgatgctgc tctgcatcaa acccccattt
    16861 ggcaatcagc aaacttcctg tttcctgcaa aactgatttt aattgtgcca caactaacgc
    16921 atcttgcatt gcttcatcaa tggtctggta acctaacaag gcacctgtat aagcgacagt
    16981 cgcatgacca gtattgacac taaatagttt gcgttcgata tatggttcta gatcatctac
    17041 atataaaacc ccttttaatt ggattttttt attttttctt tggctatcgt caatgaccca
    17101 ttctttgaaa ggttccactt ggacgaataa aggatcttca tggtgttgta atgggacgat
    17161 tcgatcgact gctgcatttg gaaagccgat atatttgtca gtaaatgatg tttccggaag
    17221 atatttatac acttcttttt ctagaaatgc tgaaccgcca atcatatttt cacaggcaat
    17281 gatatctaaa gatgttgtgc ttccttcctt ttctctttct tggatacctt ttgcaatcaa
    17341 ctcagcaata aaaggtaaga tattcgggcc aatggcggta gtaactaaat ctgcttcagc
    17401 gatttcttgg attacttttt ctggatttgt ttgattgttg attcctttga cattttttac
    17461 agtgattttt tcttttgatt catctgctaa ttcgatttcg tattcttttc tttcatttaa
    17521 agcatcaatg atcgtgtgat tcacatctac gaaagtaatc tcaaatccat tagctgccaa
    17581 aacctctcca ataaatcctc gaccgatatt tcctgcacca aaatgtactg ctctcatttt
    17641 tctctctcct taagcaattg ctagattttc cgttacttct tctttgctga gcgcgtctgc
    17701 tagctggaca acattgtcca tatcactgca ataaagtgcg atttgctgta caagttgcaa
    17761 atgctcatct ccgactcctg caataccgaa caaaacagtc gcaatctttt cttcttcttc
    17821 ggtaccaaaa tctacaccat ctggtacttg aacgacacag atgccggatt ttttcacata
    17881 tttttttgct tcatctgttc catgaggaat cgcgataaaa ttccccatat aaacagataa
    17941 cattttgtct ctatctacca tggcatcgat atattcctca tcaacgcacc ctgcttctac
    18001 aagcatctga ccgcaatagc ggatcgcttc ttctttggtt ttaaaagatc gattgagctc
    18061 gatcatgtcg aatggcaaac tttgcatgct gttttcctcc cttaagctgt ctgtttcaaa
    18121 tattgtgcaa tcgattcgta tttttgcgga ttgaccatgc cgttgactga taaatgaatg
    18181 gattgaggtg cagccatctg tactttttcc gctaaacttt ctttggctac gatcagtgaa
    18241 tcaggttcat cttttaaatc ttggatattg acttttctta acgggatagt taaatggttc
    18301 ttgtctaata tatctttgag cacggtcatc cccattgttg ctgatccgga acgatcatcg
    18361 tggacaagta gaatctcttt gatttgtttg atgtttgctg aaagaaactc ttctgctggc
    18421 tcattctcaa tttcagaaac tgatgttttc tgcttgctgc ttaattcttg aatgatttca
    18481 tcatatcgag gtgaattcaa aaagttttct actgagacaa aacgcgcgct cggtgctttt
    18541 tgttttgctc tttcttgtag ttcgacctga gtaacgatca atgtattcga gtcatctgtc
    18601 agttggctga ttgcttgatt ggtcaccgtt tgaggcaagt ctgcttcctg tacttttttt
    18661 ctcaagatcg aagcgcccat cgcacttgat cccattcctg catcacaagc aaaaatgatt
    18721 ttttgtacgc cagagaatgt agctccttga ggaatacctt gggcaccttt ggaagcttgc
    18781 ttagctgcct ttgtctcttg aacagatttt tcgaagtctt catttgtatc tttcgcatcc
    18841 gctttcaaaa ttactgcaga aataccaaac gaaacagctg cggcaacaat cacgcccaaa
    18901 ataacaggaa ggtaagaatt gaacggtgtc atcgctagaa tcgcaatgat cgaccctgga
    18961 gaagctggcg ctcttagtcc agcatccatc aattggaaaa tgaaacttcc tgacattcct
    19021 ccgcctataa ctgataagaa taataacggt ttcatcataa cgtaaggaaa atagatttca
    19081 tggatcccgc ctaaaaagtg aatgatcatg gctcctggtg ctgaagattt tgctgctcct
    19141 ttgccaaaca aagtaaaagc aagtaatacg ccaagtcctg gaccaggatt tgcttcaagc
    19201 aagaatagaa tagattttcc agcctctgct acttgttcag ttcccaatgg tgtcaaaatc
    19261 ccatgattga ttgcattatt caaaaataag atttttgctg gttcaataaa aatacttgtc
    19321 aatggaatca aatgggcaga taaaatgatc tctacacctt ttgccattgc ttgagtcaat
    19381 gcatcgacaa ctggaccaat cgcagaaaaa cctaataacg ctaatccaaa accgataaga
    19441 cctgctgaaa aattattgac tagcatttca aaaccggatt tgatccgttt ttggaatagc
    19501 tgatcaaatt tcttgatggc atatccacca agaggtccca tgatcatcgc gcctagcatc
    19561 attggaatat ctgttccgac aatcacaccc atcgttgcaa ttgccccaac aactgatccg
    19621 cggtcaccag caattacttt tccgccagta taaccgatca ataaaggaat cagataagtt
    19681 agcattgggc ttaccatggt agcgaaagcc tcatttggta aataaccatc tgggataaat
    19741 agagccgtta atacacccca ggcaatcagt gctccaatat ttggcatgac catgcttgat
    19801 aaagtactgc ctaatttttg taccctcgct tttagcgaaa tgttgttttt gggaatcgtc
    19861 cgttccattt tgtctctccc tctctccttt tgatactcta attgtatccg cttactaaaa
    19921 agagaaatag tcgtactttg gcacgaaact ttgtgccaaa ggtttgagta tgtcaacaaa
    19981 ataaaaagat ttcggtatca gaaaacttca attttaaata aaactgttat tttaattttt
    20041 ttataatatg caaacaataa cttttgttag catttaattt agatgttttc cattctatta
    20101 tgttattaaa ttttatattg gaggcctatc tatgacatta ctgatcaaaa acatgaacat
    20161 gaaacgtggt aaaaaacgtg tgattcgaca attgaatctt ttagtagaat caggagaagt
    20221 agctgctttg attgccccga acggatttgg caaaacaact ttgctgaatg gcattgccca
    20281 actgctgcca acaacttatg atcaattgtc ccttcacaac atctctccta ctgcttatac
    20341 agattatcgc cggtctttct tttttctaga atcaacgaat cagttatccg actatctatc
    20401 ggctgttgat tatttgactt tgatcaaata ctattggcag agcaaagtag aatgccaaac
    20461 tgttttccaa cgattgaaga tgactgagta ccttactttg ccgattggaa aaatgtctct
    20521 aggaatgaaa caacatgtct tgcttgctgc ttacttgatc agtgattcat ctgtcatgtt
    20581 attcgatgaa ccgttgaacg gattagaccc aggaagtatt gatatcttga atagcctcat
    20641 cagacaatgg cagaaagagg ggaagacgat tcttctttct tcccacgcta tcgcgaatat
    20701 ccaatcgatt tgttctaaag catgttttct aaaaaatgga gaaatcgtca cagaatcctc
    20761 aaatatggaa gagattttag ctatttatcg taaattatat gaggtgtaac tatgtcttta
    20821 cttacatttg aacgcaatct cttactaaaa aacaaagtaa actttttatt tcctctattg
    20881 ttggtcgtgc tctttgcatt cccacttttt tttgatcata aacttgctta tacagaattc
    20941 gatgaactga atcataacta tgaagagatg caacgtttga ttgaaacgtt gaaagaagat
    21001 gagaatgaga aagaatttgt ggaaagttta gagaaaagca acaaactgat cgaagcaatc
    21061 ttacatgcaa aaaacacagg gaatgtccag caaatggtcg aagcaactta tcattatgaa
    21121 aaagatatac tggatcgact gatttctggt caaagacaag gaattccaat cattgaacaa
    21181 caaaaacgtg tcgagttgct ccgttatatg aaagaacatc agatccaaag atattctatt
    21241 tttgacttgc cagcgcatct ctctttagca aattactacg aaaatatttt ttcaggaatg
    21301 atttctagtt ttttgatttt atgtataacc gccttgtttc tttcttccat catctcttat
    21361 gaaaaaagaa agcaggtaat ctctctagtc aatctattac cggatagtat ggtaaagaaa
    21421 catagcatcc gttttaccat ttattatggt gcagctatgc taagtctcgt gatgccattt
    21481 ttgatcgtct ctatactagt aataatcaaa aatggtttgg gagacttccg ttatcctgta
    21541 ggaacgatca ttggtcagga gatacgaatc ctgccgatgt atgagtatct tttccagagc
    21601 tttctgttcc tgttgttgtg ggtgctcttt ttaagtacaa tcagttttct tctatctgct
    21661 ttatttgaac attctctagt caatctttta ggtacacttc tttgtctttt cttagcagag
    21721 tatcgcttgt tttcttcgat tggatggata gaatcaatca gtcattactt gccaacttct
    21781 tatgttgatt ttcaaaatgt gatcatagga ggagatattt tctcgccact agcttcagaa
    21841 caggttactt tcatgaatgg tatcttgaca cttggtatat ggagtatcgt tttattattc
    21901 ataggtatgg gcactatcta tataaaaaag agctattaaa acacaaaaat aataggggcg
    21961 gtgacaaaaa ccttgtcacc gtcccctatt tcaaataaac ggttttctag aataaatagt
    22021 tttcaaaagc ttgcatccgc agtaataaga acctcccaac aaaaatagct cctcttattt
    22081 ttatcttggt tgaagttatt acttgatgca aggcagcttt ttttactgtg aacattggtt
    22141 atatatgtcc atgttcaaaa atcatttgga cttcttcaat ggtttccgca tcactgagcg
    22201 ccattacttg gtcgatgtct gaacagaaaa aggctaaatc ttggagcacc gctaactgat
    22261 gttctttttt taaagccact acaaataaga gtgtcgcgat ttttggttcc tcttttgttc
    22321 caaagtccac accatcaggc acttggacta aatagattcc ttcttctact acttcttcat
    22381 ctttgtcagt cccatgtggc aaggctacga aattcccgat atagacactc gctttttctt
    22441 gtctttcgat cattgattgg atatatgccg gtttgattcc cccttgttgg gtcatccact
    22501 ggccgatctc ggtcaacgct tctttccaat tttgatagga ttgatcgagc aatatttttt
    22561 ctgctggtag ctgtatcatc ttttcacctt catttcttta tttcttcaat caggattttc
    22621 gctaacaact gataaacgat ttctttattt cctgagcgga agatctcggt gttcaagtca
    22681 ttcatgatga ttgctccact gatctttcct aaaagaatcc cgtctttttc ttggattggc
    22741 gaaggcgcca gcataactaa catacgggac aaatcgactg gctgtctatc catcccttca
    22801 atggtcaacg gatgtttcaa attgaaaata caaaagatcg gtttagaaat caatgagctg
    22861 gatgtatgga ataaccccat atttgtatga ggaataccga taggtgcttg ttcatatcgc
    22921 ttaagcagcc ggcgatgaac cacctcttca tccgttacta gtccttcagg aaaataagga
    22981 aataagctgc gaatcgtttc ggaaatcgtc tcaggattat ccagttcttg gataaaaaaa
    23041 cgaccgagta attgattgat ttgatccatg aaatgcattg tttcctcata agtatcttct
    23101 atcatccact cgttattgct gttatacgta attttttgcc tttttttctt ctcaatatgc
    23161 gaaaaagctt cttttagctg cttgacttca tcttctagca gtagcgggga gactaaaata
    23221 tattttccac cgtatcctgg taaaatagat gtagaaataa caacatcata ttcttcttgt
    23281 aaatcgattt tttttaaatc agcaatccga tagaaatgaa tttgttggat gaagggaaaa
    23341 taccttttca atcgtagttc caacatgcta gtagaagcta atccacttgg cgaaaatcct
    23401 gcaatactga tattgctttc tttcggactt cgttccaatg aattcgcaaa atgaaggacc
    23461 atataagcga tctcttcttc ggataatttt ttttcttgaa aaatccgctc tactgcttta
    23521 cggattgcat tcgcaacttc ctcgtattga accatgattt tttctaaaat cggattggtc
    23581 agtgtctctt cttgtaaaac ggctcttgaa aacacaccag ataaatgagt caacagcatt
    23641 ttgtacaaag tttcatcttc ataaaaatgg acattcgtct cttcacttac ttcttgaatc
    23701 agttgtttta ctaaataagc aagctgtgta tcgaacgtct cttcaaagaa attctgatca
    23761 aatgagttcg tgaagtcatt taataatcct gcaaaaaaga cgatctcatt tactggataa
    23821 agttgttttg tctcagaagc gatccgtgaa aacaatcgtt tagctgtctg tagtaattct
    23881 ttgtttgcca gactattata tgtctcattt gtcagtaaat gatttttcgt cacacgctcc
    23941 atcgtcaatg tcagcatcaa aaccaaatgt tcaagctttc tatcacttag attggtcttt
    24001 ttttggatca attcttcccc gattactttg cgtgcaagag atagatgttg cggatcaacg
    24061 aatcgaaaaa aataattcag tgatgcatcg aaagaaatac tatgaaaaag ttcatattca
    24121 ttgatttcta attctaatac attcgctgtc agaagccggc ggtatttttc cggtccttcg
    24181 atctcgtatc cctggttccg aatgatttcc aaaggcagtt ggcgaatact ttcttccaac
    24241 tgtttgatat ctgcataaaa agtggtatta ctgatgcgat atgattccat gaaatgagca
    24301 agactcaacg gctctgtcgt cagtaacaac tcggctaaaa tcgcgtgttg tcgtttagca
    24361 gtggataagt cattactttt cccatctgta ttttctttta attgctctaa tacttcttct
    24421 gatgcctcaa tgacaaatga gcctctagct gcttttttca aagaagcatt taccgatttc
    24481 aatgagactt caagattatc taattcgcgg taaatagtcc gtttactgac cttcaataaa
    24541 ttcatcattt tttggataga tacaggtgac gggctactga gaaattctgc taataattgt
    24601 ttttctctgg gcgataaata catcttatat ttctcccttc acgagttttt cgtattcatt
    24661 cgtagctacc caatgttcaa ctagaactag ccgagggatc ttttcttcta gctgttcagt
    24721 tacagctagt tctctttgtg cgatataaat cgtttcttct tggattttga ttttttctat
    24781 tgattctttt gagataacaa actgtttttt cgcttttttt gctaattgac ttaaaatcgt
    24841 catgcccata gtttgtgagc cgcgtctgtt gtttttatac aaaaatacaa tattagcagg
    24901 cgacgttttt tgttctgtga tatctgctat ctcttttttt tcttcccgct ttggaaattg
    24961 gatcgcttct tttattcgtg acagcaagat atctgtttcc aaaaagtgat ccagtacata
    25021 gatttgactt tgaggtactt tttctcttgc aagacccgct agttcatgtt gcacgataac
    25081 cagctgattt acttggttat tcaattcata gatagagctg tatccgacag aaagatcgat
    25141 tttttctgct gctaatttct ttttcagcag ggcggctccc atagcacttg atcccatacc
    25201 tgagtcgcaa gcaaaaatga cggaatgaat gatagatggt tccaagattt ctcccctttc
    25261 tttgaccgtt ttgttctctt tttctttatt tggtaatttc aatagtatcg ctgcacaaat
    25321 gaaggtcaca aatgtactga ttgctatccc tatacttacc ccagctagtt gtggataagg
    25381 cgcattggca aaaatcacga gtagtgaccc aggcgataca ggtgcagcta atcccgtatc
    25441 taaccattga aatatcaacg tgccgcttgc cccaccagcc attacagcta aaaatagata
    25501 cggtcgcagc aagacaaatg gaaaatagat ttcgtgaatc ccgccaatcg catggatcat
    25561 caatgctcca ctcgcattta ctttttcctg tttttctgca aaacaaagga tagaaagtaa
    25621 gatccctaaa ccaggtccag gatttgcctc catcaaaaat aaggaagagt acccgactgt
    25681 ttgtgagaat tcgattccta aaggtgtcaa tacaccatga ttgattgcat tattgaaaaa
    25741 tagtactttt agaggttcta agatcagact gacaagtggt aacagattca ttgtgactag
    25801 ccaagacaca acggaggcga gcaacaagtg aacactattt aaaactggtt caataaaaac
    25861 gattccaatt atacaaataa cgctaccagt cagtcctact agtaggttgc tgacaagcat
    25921 ttcatatcca gccttgaatt tcacatggaa tctagaaaca aattgttcat atacgatgcc
    25981 ggcaagcggc ccgataatca tcgctcctac gatttgtgga gcattggaac gagcgatcaa
    26041 ccctaaagaa gcaattgcac cggcgactgc ccctttttgt ccccctaccg atttcccgcc
    26101 tgaataactg atcaagatgg gtaaaagcag ctggatcatc caatggcata cctcttctaa
    26161 tgtggtgtgc aaatcgccag acacaccttt tgttgctaat gacaagattc cccaagcgat
    26221 aaacagactt agatttggca tgatcatttc atttaagtga tgtccagctt ttctcagttg
    26281 tttcatagac acgcgactgc tgttgtttgc tttccatttg tattccatct ttttcaccag
    26341 acttctattt attgatggac ggattttatg tagaaaaagc agcaaacgaa aaaccgtctg
    26401 ctgctttcag ctatctggga actatttgcc tggttcagac gcaatgattg ctagttcacc
    26461 ttggaccgcc cactcagaaa cgccatttgg aataatacag cttgttcctt tttctaatgg
    26521 atatgttttt ccgtcaacga caaggtctcc tgctccttcg attaccgtca tcaatgtata
    26581 aggggcttgt tttttgaaag atgtgattcc tttaatatcc cactcatata cattgaaaaa
    26641 ctcagtttct aaataagtga cgatcgatga attccctttt cggacttctt tgatttgtaa
    26701 ttctggtgtt ttcgcaggga cagttgttac atcgatcgat tgttggatat gcagctctct
    26761 tgtttgtcct tgcgcatctt ttctgtcgta gtcatacacg cggtaagtcg tgtcagaact
    26821 ttgctgtgtt tctaaaatca tgattccttt accaatagca tggatcgttc cgcttggtac
    26881 ataaaagaaa tcaccttttt taactggtac tttcttcaaa aggtcatccc agcgcccatc
    26941 tttgatcatc tcagctagtt cttctcttgt ctttgcatga tgaccataga tgatttctgc
    27001 accaggttca gcatcgatga tataccagca ttcagttttt cctaattcac cttcatgttt
    27061 catcccatag gcatcatctg gatgtacttg tacagataga tcatcttctg cgtctaagat
    27121 cttgatcaat aatggaaaca ctggttctga aggatggcca aacaattcct tgtgttctga
    27181 ccatagttca tctaatcttt tccctttgaa ttcgccattt tctactacac tgacaccatg
    27241 aggatgcgca ctgatcgccc aatcttctcc gattttatcg ttagggatat caaaaccaaa
    27301 gactgaacgt aaacgacttc ctccccaaat tttttcttga aaaactggtt ttaaaaacat
    27361 aggttcctgc ataattcaaa ctcctctcat ttttcatagc tattatagca ttatttcggt
    27421 aataccgctt tctctttcaa aaagttttca acaatttcat ctcgacatat gagatactcc
    27481 gcacgatgat cgacaggatg tacgcgattt gacaaaaata tcaacgcttc ttgtgttttt
    27541 atatcaatca agaaaaacgt acctgtataa ccagtatgaa aaagaatcgc atgaccgtct
    27601 tctacatcct tttttaagtc ccatcctaaa gaacgatagc catttttttt cggtgtttga
    27661 tcttgcagta aagaatcaat cgtctcttct tctaaaaaac gaccattacg gatttttccc
    27721 ttgttcaaat acatttggac aaaagtaatg ctgtctttca aatcaataaa gagacccgcg
    27781 ttacctgcat gttcagccaa aactcttgct ttcggatcat gagtttgtcc acgcaaaacc
    27841 tcacccgaag gcagttgttc tgtgggaacg atttttttgg aaggaaaagg aagaaataag
    27901 ctgttcatca ttcctaatgg ttccaatact tgttcttgaa agatttccac tgcagtttct
    27961 tggtaaattt cctctagcat caacccaaga agaatcgtac cggcatctgt atattttact
    28021 tcttttccta gttgctcacc tggttctagc ttcaaataag cggcgatcaa ttcttcctga
    28081 gatagcttat ctctgtttgg tatccatgtt ttgatatcag aagtatgtgt cagtaaatga
    28141 cgcaaagtga ttgtctgatt ttcaaattca ggaagatatt tatgaagcgg ttgatcgagt
    28201 tctatctttc catcttctaa tagacgtaaa actaatgaag tggttgcaac gactttcgtc
    28261 agtgaggctg catcaaacaa cagatcttct gtcatcagct gcttttcagg aacgacttgg
    28321 gcaaagccgg ctgtaaatgt ttctacttga ttttctttta tgaaggcgac tgctacacct
    28381 ggataaaatc cttcttccat ctttttcaaa ataagctgtt tcgtgtgtgg atacatcttt
    28441 ttctcctgct tttctatcag attcttacag tccaagctta aagcttaatc tatgaggaat
    28501 caaccttttt tacacaaaaa aactgcgcga aaaaccgcgc agtttccccc ggtatactat
    28561 tttctctttc tatctctaga ataagcgata gtatgaccgt tcataaaatg acgagacatg
    28621 cgacaatcgt taagaaagaa aaagttaagc gtcgcatcga atgtcagttt tattcttttc
    28681 ttttggctca ctgacgaacc accattcgat tcctatagca tcatagattg tgagtactgt
    28741 gtgctttttg tctaagaaga aatcaactga tagttgctgc aagtgagcaa tcagtccttc
    28801 catgctttca gtatcgatca tgaatttcag aaaatcaagt ccaatcactt catctgtagg
    28861 caaatcaatt gcgcctccgc tagcttctgt caaaccaaca tggaaatctc cttcattcaa
    28921 tacatggatg ccctcttctt cattatgtac cttcaaacca agtacatgat taagaaattg
    28981 gcgttctttt gttaagttag taacatttaa atggattttt tcaaaataag aacctgttga
    29041 tagtttactg aatttttctt cagattttct taataatgct tcttgatcca ttgtttctgg
    29101 aatttctctt tggtcgttct tattctcttc atggtaaatt tccaattcat tctcttctgg
    29161 gtctacgagc aaaatcccca tacgtccatg atcttctaaa gcttctttga ttggataatt
    29221 cattttacgg atacgacaaa ggatatctgc catttcttct gtgctaggga taatcaatga
    29281 aaaacggttc agctttttga tcacccccat gtgatcattc gctctaggac tttcttccag
    29341 cagcaacatt tcacttccag cttcttctgt tcctaaaatg gctaattcgt tttcttctct
    29401 tttcaaatca aagccaacaa cgttcttgta aaaatcaatc attttatctc ggtctttcac
    29461 ccgaatggct acacttttta aataggtggc agcccccaat tgaaaatctt tcatatttct
    29521 tgctcccctc tacgtcaagc tttattttaa cgatggattc ttcttctttc aagcaataac
    29581 tttgattttg ttaaaaaaaa tgtgagaaaa ttcgcatttt gcttgttttt tgtaaattaa
    29641 tgagtaaaaa ctcttataaa aagaggttgt gaaaaagctg ttctgcatca agtaataaga
    29701 acagccaaac aaaaatagct cctcctattt ttcgtttggt tgggcttatt accgcagatg
    29761 caagcttttg aacaccgttt aaggaagagg ttgtgaaaaa gtcgcttagt ttcgagtagt
    29821 aagaaaaatt ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag
    29881 aaactggctt ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actccgagtc
    29941 acaaggagca atttcaaaaa acagcttctc aggtttgttg actattgtga cttgtgaccg
    30001 aggagttggc aggagaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga
    30061 gtattagaga tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata
    30121 ccgaaggaca gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg
    30181 tcacagcccc taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa
    30241 actgactgtt ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc
    30301 ctgtttctac gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag
    30361 acaagcggtg agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt
    30421 gaatcagtat ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat
    30481 ctggatttgc taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat
    30541 tgcttgcatc ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt
    30601 ctacgtgagc agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat
    30661 atttgatatt gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa
    30721 actgtgcgcg caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac
    30781 tacctccaga aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac
    30841 cagtcggacc tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca
    30901 ttagcaagtt atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg
    30961 tatctttttc ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt
    31021 caaatacccg ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt
    31081 gagtgattgg ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg
    31141 tcatgttccc atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat
    31201 ttttaacgaa attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag
    31261 cggcatacag attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga
    31321 atgttttaac aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta
    31381 aacttttttg ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga
    31441 tcaagcttaa agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca
    31501 tcatcacgat gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct
    31561 gcagcgtgct tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg
    31621 tatgtgtatc ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta
    31681 attcataaac tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata
    31741 cagcagaaat caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt
    31801 ctatcggaaa ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac
    31861 ctttgaatat ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca
    31921 cggctgctat tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc
    31981 caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat
    32041 ttctgccagg tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg
    32101 ttttactcat gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc
    32161 gtttgttgct aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac
    32221 aaggatcata tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact
    32281 ttctttcata ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc
    32341 tgagaaggaa tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc
    32401 cagacgctgt ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa
    32461 gccatcttcc atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat
    32521 catttcatct gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa
    32581 cagaactgct ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc
    32641 actaatgtcc gttccattta tttggatcga accagattcg atgtcatata atcgagggat
    32701 caaattgact aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc
    32761 cgacttggct tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata
    32821 attgacatga ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt
    32881 ttcagggtca tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc
    32941 ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg
    33001 catcgcataa gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta
    33061 gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg
    33121 catcatcaat gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc
    33181 taaatcaaaa cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc
    33241 tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa
    33301 taatggaaca gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac
    33361 taagaagatt tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa
    33421 cgtgattggt gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt
    33481 cacatcattg gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt
    33541 tgtcagactc tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga
    33601 ttcacgagta gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc
    33661 gatcatcaca aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc
    33721 aataatattg gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa
    33781 aactgcagct gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt
    33841 cccttctttc tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact
    33901 gattgcttgc tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat
    33961 tgttgtaaat acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt
    34021 tactttcagc ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc
    34081 tttatgtgga tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc
    34141 acggtaatcc atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat
    34201 ctcataaatc attttagaaa aataggattc gaatcctttg aatagatcac catcggattc
    34261 tttcattagt tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa
    34321 ataaagatcc tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa
    34381 tttgacgata ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc
    34441 agcctcgatt agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat
    34501 atggattctc ctttctttca agatgacaca ttgtcatatg acgctatgtc attataacac
    34561 ctcctacctg ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa
    34621 aaagtgataa gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac
    34681 atactaaaca aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat
    34741 tgcctctcga tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa
    34801 accacttaag ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc
    34861 tagatccaaa gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag
    34921 ccgatcaact tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc
    34981 ttcagtcgag tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat
    35041 cgtgtcacag cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca
    35101 atatgacttg atagcctttt ctttttcgag taaactgatt ttgttagtca tagttataac
    35161 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
    35221 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
    35281 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
    35341 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
    35401 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
    35461 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
    35521 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
    35581 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
    35641 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
    35701 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
    35761 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
    35821 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg tgccttggat
    35881 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
    35941 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
    36001 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
    36061 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
    36121 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
    36181 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
    36241 gggatcaggt ttaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
    36301 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
    36361 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
    36421 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
    36481 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
    36541 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
    36601 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
    36661 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
    36721 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
    36781 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
    36841 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
    36901 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
    36961 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
    37021 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
    37081 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
    37141 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
    37201 ctactgtctt taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
    37261 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
    37321 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
    37381 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
    37441 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
    37501 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
    37561 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
    37621 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
    37681 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
    37741 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
    37801 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
    37861 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
    37921 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
    37981 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
    38041 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
    38101 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
    38161 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
    38221 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
    38281 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
    38341 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
    38401 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
    38461 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
    38521 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
    38581 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
    38641 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
    38701 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
    38761 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
    38821 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
    38881 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
    38941 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
    39001 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
    39061 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
    39121 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
    39181 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
    39241 gtactgactt tcataagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
    39301 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
    39361 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
    39421 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
    39481 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
    39541 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
    39601 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
    39661 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
    39721 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
    39781 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
    39841 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
    39901 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
    39961 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
    40021 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    40081 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    40141 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    40201 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    40261 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    40321 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    40381 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    40441 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    40501 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    40561 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    40621 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    40681 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    40741 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    40801 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    40861 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    40921 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    40981 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    41041 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    41101 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    41161 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    41221 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    41281 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    41341 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    41401 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    41461 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    41521 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    41581 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    41641 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    41701 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    41761 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    41821 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    41881 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    41941 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    42001 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    42061 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    42121 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    42181 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    42241 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    42301 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    42361 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    42421 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    42481 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    42541 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    42601 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    42661 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    42721 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    42781 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    42841 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    42901 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    42961 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    43021 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    43081 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    43141 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    43201 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    43261 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    43321 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    43381 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    43441 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    43501 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    43561 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    43621 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    43681 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    43741 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    43801 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    43861 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    43921 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    43981 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    44041 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    44101 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    44161 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    44221 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    44281 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    44341 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    44401 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    44461 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    44521 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    44581 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    44641 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    44701 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    44761 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    44821 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    44881 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    44941 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    45001 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    45061 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    45121 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    45181 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    45241 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    45301 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    45361 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    45421 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    45481 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    45541 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    45601 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    45661 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    45721 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    45781 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    45841 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    45901 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccctttggct acactgttcg
    45961 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    46021 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    46081 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    46141 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    46201 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    46261 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    46321 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    46381 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    46441 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    46501 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    46561 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    46621 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    46681 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    46741 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    46801 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    46861 tcaagcagtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    46921 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    46981 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    47041 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    47101 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    47161 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    47221 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    47281 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    47341 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    47401 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    47461 attatttgct aaaagggttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    47521 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    47581 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    47641 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    47701 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    47761 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    47821 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    47881 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    47941 gaactttatt gaaaataggc ggtagcgtga tgggactttc tttcatccta ccagctgttt
    48001 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    48061 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcgta ggcgaaatat
    48121 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    48181 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    48241 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    48301 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    48361 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    48421 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    48481 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    48541 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    48601 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    48661 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    48721 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    48781 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    48841 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    48901 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    48961 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    49021 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    49081 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    49141 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    49201 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    49261 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    49321 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    49381 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    49441 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    49501 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    49561 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    49621 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    49681 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    49741 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    49801 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    49861 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    49921 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    49981 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    50041 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    50101 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    50161 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    50221 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    50281 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    50341 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    50401 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    50461 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    50521 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    50581 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    50641 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    50701 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    50761 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    50821 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    50881 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    50941 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    51001 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    51061 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    51121 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    51181 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    51241 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    51301 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    51361 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    51421 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    51481 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    51541 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    51601 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    51661 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    51721 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    51781 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    51841 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    51901 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    51961 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    52021 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    52081 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    52141 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    52201 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    52261 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    52321 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    52381 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    52441 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    52501 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    52561 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    52621 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    52681 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    52741 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    52801 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaagt ttagtctcaa
    52861 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    52921 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    52981 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    53041 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    53101 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    53161 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    53221 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    53281 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    53341 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    53401 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    53461 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    53521 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    53581 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    53641 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    53701 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    53761 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    53821 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    53881 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    53941 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    54001 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    54061 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    54121 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    54181 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    54241 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    54301 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    54361 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    54421 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    54481 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    54541 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    54601 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    54661 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    54721 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    54781 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    54841 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    54901 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    54961 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    55021 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    55081 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    55141 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    55201 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    55261 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    55321 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    55381 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    55441 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    55501 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    55561 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    55621 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    55681 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    55741 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    55801 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    55861 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    55921 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    55981 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    56041 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    56101 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    56161 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    56221 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    56281 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    56341 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    56401 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    56461 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    56521 tttgtttctg tcctgattgg tgcaataaca tttaccaatt gagcaagaca agcaatcttt
    56581 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    56641 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    56701 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    56761 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    56821 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    56881 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    56941 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    57001 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    57061 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    57121 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    57181 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    57241 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    57301 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    57361 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    57421 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    57481 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    57541 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    57601 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    57661 atataaatgt taaacttact tattttttat cgattttaat taaccaaaat attaataatg
    57721 attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac
    57781 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
    57841 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
    57901 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
    57961 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
    58021 ccttcgtgtc gataaaatcg atatcagttt tccagaaaaa atattcaatg cttttgatac
    58081 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
    58141 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
    58201 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
    58261 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
    58321 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
    58381 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
    58441 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
    58501 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
    58561 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
    58621 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
    58681 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
    58741 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
    58801 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
    58861 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
    58921 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
    58981 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
    59041 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
    59101 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
    59161 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
    59221 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
    59281 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
    59341 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
    59401 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
    59461 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
    59521 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
    59581 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
    59641 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
    59701 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
    59761 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
    59821 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
    59881 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
    59941 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
    60001 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
    60061 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
    60121 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
    60181 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
    60241 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
    60301 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
    60361 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
    60421 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
    60481 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
    60541 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
    60601 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
    60661 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
    60721 cacttggatt aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
    60781 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
    60841 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
    60901 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
    60961 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
    61021 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
    61081 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
    61141 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
    61201 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
    61261 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
    61321 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
    61381 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
    61441 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
    61501 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
    61561 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
    61621 ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt
    61681 taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat
    61741 cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt
    61801 cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata
    61861 gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc
    61921 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
    61981 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
    62041 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
    62101 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
    62161 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcgtgaga
    62221 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
    62281 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
    62341 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
    62401 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
    62461 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
    62521 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
    62581 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
    62641 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
    62701 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
    62761 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
    62821 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
    62881 cataagaagt ggttctctta cagctggcct gaatatcagc caaaagcgca taaaggacta
    62941 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
    63001 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
    63061 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
    63121 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
    63181 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
    63241 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
    63301 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
    63361 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
    63421 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
    63481 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
    63541 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
    63601 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
    63661 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
    63721 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
    63781 cttcaagata ccttcgctct ttttctttat gatagcccgt cggtaaaagt gtcactttgt
    63841 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
    63901 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
    63961 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
    64021 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
    64081 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
    64141 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
    64201 cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt
    64261 cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac
    64321 ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc
    64381 tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg
    64441 tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca
    64501 aagtctctta ttttaaattg aatacattat aatgatactg taaatatatc tttattatta
    64561 gtgaatggag tcaagtccac aatcttgtgt aaaagttcaa cctcatcaaa agcacaatgt
    64621 tttgcgttgt gaacctaact gccatgcctt tctttctgag gagagaggtc aggagcagta
    64681 gcgcagcgac tcttgatgtc tctcctcaga aagaagagac tcgcacaggt tcatggtgct
    64741 aaatgttatt cgaattaaat tctaaatatt ttcgtggaga agccagccac tcagtgtctt
    64801 tttccatcaa aagtgttcca attatccgag tgatggaatt gatatttgga aaaatccgaa
    64861 tcactcgttc tctccggcga atctcttcgt tcaaccgttc caacacattg gttgtacgaa
    64921 tccttcggcg gatattttca ggaaaagaca aaatttgaat agcatcttca aagccgtttt
    64981 ctaaaatgtc gcaagcagca cttaattttg aatcacaacc atatttttcc aagaagtgct
    65041 cttttctttc acgtgtcaat tcaagttcag aggctttaaa gatactcttc aactcgtctt
    65101 ttacatcaga agaaactttc tttggcagct tatcaaaaat atttctcaaa aagtgggctt
    65161 ggcatctttg ccagctcgca cctaaaaagt tcttgcgaac agccttaact aaccctttgt
    65221 gtgcatcaga aatgaccagt tttacaccga acaaacctcg ttgaacgaac gattgaaaaa
    65281 cggtatccca attatcttcc gattcgccgt cttgaacatc aaaaccaaga atcgtacgtc
    65341 gtccatcaga atcaatgccg ataccaatat atacaccttt tgagacaata cgatgatttt
    65401 cccttacttt catatacagg gcatcacaca tgaaaaatgg atattccgag ccttctaggc
    65461 tccggtttct ccactcaaaa acaaatggat ccaactgttt catcagattt gaaacgaatg
    65521 atttagaaac agtcgcacca tccgttagca gttcaactgt tttagttact ttgcgagtgg
    65581 agacaccaga aatgaccatt tcaatcatgg cagcgatcaa agctttttcg cttcgctgat
    65641 atctttcaaa gatagtagga ctaaacttac cgtctctggt tcgtggaaca tgaagttcta
    65701 acgtaccaat tcgagttgta aacgaacgat cgtagtaacc atttctttgt gattgccgtt
    65761 cttctgaacg ttcataggca tttgcctgaa tatattgatt tcgttgttct tccatcagct
    65821 gattaaacat gatagtcata agtgtttttg cggtatcatt cgcaccgctt tcgtttataa
    65881 ttgcttgaat ttcttcgctt tcaaaagtaa aatgtacttg agtcattgat atcagtccta
    65941 ttcgagtatg ttttctgcaa aaaacattgt actcctaact gagatcaatg gcttttttga
    66001 tgcttcactt tta
//
LOCUS       DACDIP010000010        59072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDIP010000010 DACDIP010000000
VERSION     DACDIP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.010"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            647..964
                     /locus_tag="IV257_000747"
     CDS             647..964
                     /locus_tag="IV257_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8813895.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IV257_000748"
     CDS             complement(1055..1420)
                     /locus_tag="IV257_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813896.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IV257_000749"
     CDS             1628..2110
                     /locus_tag="IV257_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813897.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IV257_000750"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IV257_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8813898.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3484..4758)
                     /locus_tag="IV257_000751"
     CDS             complement(3484..4758)
                     /locus_tag="IV257_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8813899.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5031..7502
                     /locus_tag="IV257_000752"
     CDS             5031..7502
                     /locus_tag="IV257_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8813900.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7605..9341)
                     /locus_tag="IV257_000753"
     CDS             complement(7605..9341)
                     /locus_tag="IV257_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8813901.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9338..10042)
                     /locus_tag="IV257_000754"
     CDS             complement(9338..10042)
                     /locus_tag="IV257_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8813902.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10173..10676
                     /locus_tag="IV257_000755"
     CDS             10173..10676
                     /locus_tag="IV257_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8813903.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10636..10974
                     /locus_tag="IV257_000756"
     CDS             10636..10974
                     /locus_tag="IV257_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8813904.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10995..12413
                     /gene="ffh"
                     /locus_tag="IV257_000757"
     CDS             10995..12413
                     /gene="ffh"
                     /locus_tag="IV257_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8813905.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12525..13103
                     /locus_tag="IV257_000758"
     CDS             12525..13103
                     /locus_tag="IV257_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8813906.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13107..13628
                     /locus_tag="IV257_000759"
     CDS             13107..13628
                     /locus_tag="IV257_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8813907.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13707..14261)
                     /locus_tag="IV257_000760"
     CDS             complement(13707..14261)
                     /locus_tag="IV257_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8813908.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14514..14789
                     /gene="rpsP"
                     /locus_tag="IV257_000761"
     CDS             14514..14789
                     /gene="rpsP"
                     /locus_tag="IV257_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8813909.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14801..15052
                     /locus_tag="IV257_000762"
     CDS             14801..15052
                     /locus_tag="IV257_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8813910.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15177..15968
                     /locus_tag="IV257_000763"
     CDS             15177..15968
                     /locus_tag="IV257_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813911.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16138..16659
                     /gene="rimM"
                     /locus_tag="IV257_000764"
     CDS             16138..16659
                     /gene="rimM"
                     /locus_tag="IV257_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8813912.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16649..17410
                     /gene="trmD"
                     /locus_tag="IV257_000765"
     CDS             16649..17410
                     /gene="trmD"
                     /locus_tag="IV257_000765"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8813913.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17546..17893
                     /gene="rplS"
                     /locus_tag="IV257_000766"
     CDS             17546..17893
                     /gene="rplS"
                     /locus_tag="IV257_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8813914.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(18025..19161)
                     /locus_tag="IV257_000767"
     CDS             complement(18025..19161)
                     /locus_tag="IV257_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8813915.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPNLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKMPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPKGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(19278..19970)
                     /locus_tag="IV257_000768"
     CDS             complement(19278..19970)
                     /locus_tag="IV257_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813916.1"
                     /translation="MKKVILGLVFGAFFLTGCSQSNVVDNTGKTNEKDSSVTLSESTT
                     QSSKNSQEVDGPLLKVGQWTKEKRDTSGKIELIGTASPQSDIPLGDIGIYISNIKLLK
                     YSNYADPSAAGYYDANNHKDSEGNFYGLQIAFKVKNNSEVDYGYNGLAHAILDNGQQI
                     DFSSDDLMHTMTTNSFFKKTESKEFYSLAYLDPSKVNDITKVTIKTGVLYNPSDYSTV
                     AESAESTFNINR"
     gene            complement(20108..20761)
                     /locus_tag="IV257_000769"
     CDS             complement(20108..20761)
                     /locus_tag="IV257_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813917.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     GQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(20811..21239)
                     /locus_tag="IV257_000770"
     CDS             complement(20811..21239)
                     /locus_tag="IV257_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8813918.1"
                     /translation="MEMDTINLVEELKRKYRSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIDELKNLYGLPESAYRFLI"
     gene            complement(21244..21618)
                     /locus_tag="IV257_000771"
     CDS             complement(21244..21618)
                     /locus_tag="IV257_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8813919.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            21903..22088
                     /locus_tag="IV257_000772"
     CDS             21903..22088
                     /locus_tag="IV257_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8813920.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            22101..22349
                     /locus_tag="IV257_000773"
     CDS             22101..22349
                     /locus_tag="IV257_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813921.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKITPKEFIEKHLVDL"
     gene            complement(22648..23313)
                     /locus_tag="IV257_000774"
     CDS             complement(22648..23313)
                     /locus_tag="IV257_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813922.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDHLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            23368..24066
                     /locus_tag="IV257_000775"
     CDS             23368..24066
                     /locus_tag="IV257_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8813923.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLKEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNIKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            24244..24585
                     /locus_tag="IV257_000776"
     CDS             24244..24585
                     /locus_tag="IV257_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813924.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            24578..25249
                     /locus_tag="IV257_000777"
     CDS             24578..25249
                     /locus_tag="IV257_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8813925.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            25255..25941
                     /locus_tag="IV257_000778"
     CDS             25255..25941
                     /locus_tag="IV257_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813926.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVTNFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            25944..26171
                     /locus_tag="IV257_000779"
     CDS             25944..26171
                     /locus_tag="IV257_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813927.1"
                     /translation="MINLNAVAFEQTWRTKYKKMSPRDKLFLEIMTFAFIGTQAEQSD
                     ISVEKIKTNRLVNGITETCYQYTIIVVDEEE"
     gene            26174..27001
                     /locus_tag="IV257_000780"
     CDS             26174..27001
                     /locus_tag="IV257_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HAP8813928.1"
                     /translation="MAERRMFAKTIIDSDAFLDMPLSTQALYFHLSMRADDDGFINNP
                     KKIQRMVGCGDDDLKLLMAKRFILVFESGVIVIKHWKIHNYIRNDRYKPTLYQDEKAL
                     LADKDNKAYTFAEELSKHDEKLGIPDDNQAVYQMDTQVRLGKDRLGKDSKEIKDLTPS
                     KKSKAKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESSGKTYKNYL
                     ATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            27013..27282
                     /locus_tag="IV257_000781"
     CDS             27013..27282
                     /locus_tag="IV257_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813929.1"
                     /translation="MKIDYLELINEIANYKTGEEIDILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDATQLRNLAYTKLLFVDCIKGLQKHLSELEQV"
     gene            27444..27746
                     /locus_tag="IV257_000782"
     CDS             27444..27746
                     /locus_tag="IV257_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813930.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            27743..28054
                     /locus_tag="IV257_000783"
     CDS             27743..28054
                     /locus_tag="IV257_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813931.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            28051..28269
                     /locus_tag="IV257_000784"
     CDS             28051..28269
                     /locus_tag="IV257_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAP8813932.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            28280..29095
                     /locus_tag="IV257_000785"
     CDS             28280..29095
                     /locus_tag="IV257_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP8813933.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            29234..29590
                     /locus_tag="IV257_000786"
     CDS             29234..29590
                     /locus_tag="IV257_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8813934.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            29550..29795
                     /locus_tag="IV257_000787"
     CDS             29550..29795
                     /locus_tag="IV257_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813935.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            29792..30190
                     /locus_tag="IV257_000788"
     CDS             29792..30190
                     /locus_tag="IV257_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813936.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            30187..30525
                     /locus_tag="IV257_000789"
     CDS             30187..30525
                     /locus_tag="IV257_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813937.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            30522..30752
                     /locus_tag="IV257_000790"
     CDS             30522..30752
                     /locus_tag="IV257_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813938.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            30759..30962
                     /locus_tag="IV257_000791"
     CDS             30759..30962
                     /locus_tag="IV257_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP8813939.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            30959..31252
                     /locus_tag="IV257_000792"
     CDS             30959..31252
                     /locus_tag="IV257_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813940.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            31282..31722
                     /locus_tag="IV257_000793"
     CDS             31282..31722
                     /locus_tag="IV257_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8813941.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            31797..32264
                     /locus_tag="IV257_000794"
     CDS             31797..32264
                     /locus_tag="IV257_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP8813942.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            32541..32777
                     /locus_tag="IV257_000795"
     CDS             32541..32777
                     /locus_tag="IV257_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813943.1"
                     /translation="MYWRVYKMNNASEKIDQLTILVNQRIEDIIKEFEGEKIKSIKLT
                     LMDHKFELNSTKRKIVELIEKEIAVQINEQQLTN"
     gene            32801..33013
                     /locus_tag="IV257_000796"
     CDS             32801..33013
                     /locus_tag="IV257_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813944.1"
                     /translation="MDKESARELYLSKFKRNNKPEEVFGSNVKEVGEKLTQLLKEEDL
                     TYDEAYASLQYCYNLLKYESNFLKLQ"
     gene            complement(32991..33464)
                     /locus_tag="IV257_000797"
     CDS             complement(32991..33464)
                     /locus_tag="IV257_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813945.1"
                     /translation="MNLSEVQAAYFILNCLDPDNYTVKLYVKNGCKIFSADFLKLSET
                     GWSVIQESLDEDFDFSNPKISIASGAEKGFLWAKAAKTWNDSFSYPRQSVKLLDHPQL
                     LLEKIKAFIGNLQIEDYREFSTLAYNTEYIDGKKFNFCLLPESNYELFTIEVSEN"
     gene            33597..33812
                     /locus_tag="IV257_000798"
     CDS             33597..33812
                     /locus_tag="IV257_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813946.1"
                     /translation="MKTRETRAKRNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYG
                     NVVSVLSVHHQASVTVAEVEDAAVNGE"
     gene            34004..34216
                     /locus_tag="IV257_000799"
     CDS             34004..34216
                     /locus_tag="IV257_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813947.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQSKYFYDQL
                     ILQGFNEGQAMEFMMRTFSASNQQKE"
     gene            34223..34450
                     /locus_tag="IV257_000800"
     CDS             34223..34450
                     /locus_tag="IV257_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813948.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIIEMTREAT
                     PNEIDKYNLRYCGHGYWKDEYIQQNIERYIK"
     gene            34447..34731
                     /locus_tag="IV257_000801"
     CDS             34447..34731
                     /locus_tag="IV257_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813949.1"
                     /translation="MNDNYDYIKLIEKIRAEKDMDELANLFMNIISLVGLKMDEVAAL
                     NYFIAEQTIRAEHNAKFLKDRLDLDVKGLGVEGIFKVQEALVNVYVEKMQ"
     gene            34772..35344
                     /locus_tag="IV257_000802"
     CDS             34772..35344
                     /locus_tag="IV257_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813950.1"
                     /translation="MKLKDYIREGYNIVTTPNLAYKIQEDYPNVLVFTDRALDAIPIG
                     KLLVDRYYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWHTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            35345..35656
                     /locus_tag="IV257_000803"
     CDS             35345..35656
                     /locus_tag="IV257_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8813951.1"
                     /translation="MIDVSTKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            35647..35907
                     /locus_tag="IV257_000804"
     CDS             35647..35907
                     /locus_tag="IV257_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813952.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEETTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            36075..36629
                     /locus_tag="IV257_000805"
     CDS             36075..36629
                     /locus_tag="IV257_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8813953.1"
                     /translation="MIGLNSKLVGTRMKQKRIEKGLTQADVATKSGISSRYYGSIENG
                     KNSPSIETLNSISIVIGCSLHFLLNDRMDDMEKRVELEKKRLQKIFDKIPHDKLNLVE
                     GLIIQAARLRVLLDDNWKDIIENGEYEKFKQSENQIAYDRKRPIVENYDNRDKTYQAI
                     VKQLTDLLPQGTRADKKSKLLGRK"
     gene            36632..38323
                     /locus_tag="IV257_000806"
     CDS             36632..38323
                     /locus_tag="IV257_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8813954.1"
                     /translation="MLFNQHVDQYIRKWKSGELILNEKRIQLLKLIEKEILPRDDIYY
                     FDEEQINNYIEFSQAWYFELDEWEKFISAFIFLFYKEDDEVVFDEFVINMGRGGGKNG
                     FISTLANYFVSSLHGIDYYDVSIVANSEKQAKRSFQECYRVINKKGNEDLKEEFEAYK
                     SSLTGLETQSVFEYKTSNASSQDGGREGAVIYDEYHEMENTDIVDVFSGGLGKVDHGR
                     QFFIGTKGFVREGYFDIKYRECEDILNGLSEFKGVFPYICELDAIEEMDDPKNWPKAN
                     PALQPPLNKRGKRLFNEVMKKYKKLAKEPSGRSAFVTKRMNFLEDNMENSVASREEIM
                     ATNRPFFELDTVPIGSLDFGSVRDFATCGLLFKNRKKSEYAFKSFTFAMKNFCDVHYG
                     YSLKEQLVGSEKRAPIKTWEKMGLMKVIDEPSLNPKYIVDWFIQAREEYGVRVIVVDN
                     YKADILGPMLEKEGFEVVRLKRPSSLHPLLAPRIEDGFANHKFIFGDNPLMRWFTNNV
                     YVKETQAGKQFLKKEEVKRKTDGFQAFVHALYKATELDEQIDYDDAFDMLDEIEF"
     gene            38355..39302
                     /locus_tag="IV257_000807"
     CDS             38355..39302
                     /locus_tag="IV257_000807"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813955.1"
                     /translation="MRIKGKNISWVFYMLGILAFTFVAIKLSEIIIPNDSVVSKNLVL
                     ERAKLIISILGPALTLIVFVRTIQTQLESSKNLIKDNVSKDFYNLLNLFKSIQDKMNS
                     ELFSTIEKRIKVPINNINTNLDVRENKETTKEVQEDLKEYSASLNKVLSESVKKINEI
                     FEDNYKDIGSYLKVFHRILKILNNRYESKDISHEEYKMYVGILRSQIEDYEFYIILIN
                     SLFTLRGTGMGIELIETSFFGDELDIDLKQHFEHPEFLTEIIKDNFVEKKSYRESVKT
                     RKKNRKLFENRKINDNLLSLLRIRYTLQDGNKIIISNGV"
     gene            39351..40523
                     /locus_tag="IV257_000808"
     CDS             39351..40523
                     /locus_tag="IV257_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8813956.1"
                     /translation="MSLFDIFKQSIKNEDPSDWIPDFVFGDEESARAYLKIMAKSTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWAMSDVIYMEYNNEELERFTR
                     GLFEDYAELFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDKIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIADVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            40510..41214
                     /locus_tag="IV257_000809"
     CDS             40510..41214
                     /locus_tag="IV257_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8813957.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            41219..42379
                     /locus_tag="IV257_000810"
     CDS             41219..42379
                     /locus_tag="IV257_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8813958.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTVEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVEYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            42455..42733
                     /locus_tag="IV257_000811"
     CDS             42455..42733
                     /locus_tag="IV257_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8813959.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            42714..43085
                     /locus_tag="IV257_000812"
     CDS             42714..43085
                     /locus_tag="IV257_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8813960.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            43101..43505
                     /locus_tag="IV257_000813"
     CDS             43101..43505
                     /locus_tag="IV257_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813961.1"
                     /translation="MSNVKFEGVQETIQALEKKFGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIDEVVRKNATYRNYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSEPLYFDAVGEEVKKRL"
     gene            43502..43912
                     /locus_tag="IV257_000814"
     CDS             43502..43912
                     /locus_tag="IV257_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813962.1"
                     /translation="MKDMLNIIYEALCLNEYIRSMTYNENTEQYRIKFYEQPETADKT
                     GPMITLRPVDTPNEAYHGSNKELSVEHMVQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFSETKRFVDARRYEGNTKIYDTEY"
     gene            43973..44560
                     /locus_tag="IV257_000815"
     CDS             43973..44560
                     /locus_tag="IV257_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8813963.1"
                     /translation="MTLVGFKKMTIGIFDSTGKIPAANLYVIEGEQDKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITALKEMVFPAAGE"
     gene            44633..45100
                     /locus_tag="IV257_000816"
     CDS             44633..45100
                     /locus_tag="IV257_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP8813964.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            45146..45454
                     /locus_tag="IV257_000817"
     CDS             45146..45454
                     /locus_tag="IV257_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813965.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            45649..52413
                     /locus_tag="IV257_000818"
     CDS             45649..52413
                     /locus_tag="IV257_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8813966.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGITGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRDLATKLPASHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKMLKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKALDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDNTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDIVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPNDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPNAKGTNFHPG
                     GAAMVNDQKGPTYEELITLPSGEAFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYADG
                     IGNITTVSSTLNLDALILAINELTTVLRTQLMAETTNAKDTKLSDETKVADPIIPGSL
                     SEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGVAW
                     NKNLLNGWNSLTGTFISTINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGS
                     FIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVG
                     GMGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEE
                     YVQMPDGRGFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLL
                     DDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDGA
                     VNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYT
                     AAANYAFEKFPSKIAYVITNGRVRDRMGLSGTGSSGQWTNWPDGDHFDHIHLNGSMGS
                     GDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGND
                     GLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGK
                     GHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGDDKKRSNTT
                     NTADNSAELVTLIKQQQKQHNELMMILRAILGKDLSLKSSDIGQAANHYMGSDLNKLR
                     YTNGGV"
     gene            52417..53238
                     /locus_tag="IV257_000819"
     CDS             52417..53238
                     /locus_tag="IV257_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HAP8813967.1"
                     /translation="MFYKLQFNQNGKLFDPQRKEKIVCKEIKRQAPVYEVNYEDFEGT
                     NGSREANASFRPFELVLTFDIFYKNKHDKELLLTEFYELIFVGYQYYISYDLSPGKRF
                     KVNPTNFEITEEENDYSTIEITFSIPSGSAESSATTLTDFNLEEEWQFSQGLVAEDYK
                     YTHQTSHFIIYNAGSFEIDPREHYLRIALEGESEGNVTIFNKTTGDRFIYYPSLSTNL
                     GQTLVLDGVIPKLNGVSCGINTNHGLINLVEGVNEIEIQNITRVKSSWDFRFLYK"
     gene            53247..54290
                     /locus_tag="IV257_000820"
     CDS             53247..54290
                     /locus_tag="IV257_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813968.1"
                     /translation="MTELIIRNYEQTKEEILVGYDKGSFYENWQQNETWEIGFTVTSN
                     SLNQEVFDLVEYESSVFYNGQEFVIKEMTRKALGQLLTKQVVATHIYYTVQDGYQYNT
                     VTGAKSISQLLTHVFSAGSRGFTWEVIDPNKKFLTVEQENFGNANYLKLINEILSDYN
                     AVVIPNNKHLTFYPASEYGQQTEEQIRYKYNTDEVSFDIDTYSLKTQIKGYGKLKDGA
                     NTENPKDSDYIFTPITYTSPESQKWGIRIQDPVKDERYTVSGNMLERLKTDLQDYPNI
                     SGSVTLKWKISPNKGDHVPFIYEPLNINTYIQVVGIKTYPAIPNKPPEITLSNTKKTM
                     TSILAEMAKKGVI"
     gene            54290..55321
                     /locus_tag="IV257_000821"
     CDS             54290..55321
                     /locus_tag="IV257_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813969.1"
                     /translation="MGLLKLISNRISTEWKEKFNKNIDYLNDLEKKLSDQDKSMNSRI
                     DNLVLHSGGESPNEVVDARVNNKGETFPTLHGRLVEHETLTDEQISELNTNMDSQKEQ
                     VQQLNKSVQQIIGGYNEPINIYVSKDGSDILGDGSEEKPFLTIQTAVNNVPLITTSQI
                     TIWVGSGAYLEDIMIRNLNFTSFLIRPIDNFDTIDPLKSDLPVKIRSICFTACKGYCQ
                     VAGMQIVDTANGADYGIRNEQSGYMAINRCKFAENTKTLARYNAVYVGGTSKINMYGD
                     TTLINQKVAIYAVLMGEIFVSAFGSGNDVGILCENGTVRGTVSTSFATTPTKTTGYGL
                     IITKGTVLQ"
     gene            55321..57423
                     /locus_tag="IV257_000822"
     CDS             55321..57423
                     /locus_tag="IV257_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP8813970.1"
                     /translation="MVYKTNESIIMIQAEATSPNRTNVVFWSHDRGTAKLRMKLVRKN
                     GIPQSLPEGTTVPIRLMLKSATAEGGYGKHDYLATIEDRVNGIVSIVLEDNILGYVGI
                     VEGSVYIDFPNDRSLDTAGRFTFDIKRSPIDDSTPELEDYYFNGFSQTIDKIEKILAD
                     GKQEIEQKITESETQIDGKLKETSNKITKANQDVATINTNIDKANDRIDQTNQQISDL
                     GKLKKMYSNSIDFGNYDYSGNPNLMANINADSFSQGSGALSVVDDGDEVVITLDPNHK
                     LEIFKAKSQPALVVGKTYTMSVEIMLEDDFTGDPSNISLRYIKMPSWLTVLYTPYTLT
                     NTKGVWQKLTVTAKMTTAIDNAESWFIMLQNKDANNSLSGKLRLRHAKLEEGSTATPF
                     QPNLLAEPYNMCREYPNENIADPKVKFPIESGDHQIYQGYTEEELMIGQTYTITLKGT
                     KPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVAMPKLFRVYQYPRSTVGA
                     CQIDWLKIEKGNTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKGLNNAVNVYD
                     PQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATFIKHGKEVVVNG
                     TVKFKKAYAFGVPLDDEVPDEFIARIVHGMLTGQSGTNSVSKAMYVQKDLGKIMTNSE
                     FAANEWFTFHGNYWVGVK"
     gene            57424..57870
                     /locus_tag="IV257_000823"
     CDS             57424..57870
                     /locus_tag="IV257_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813971.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGLRADGEPL
                     TIADQMFDPKLNQWIVLANTLDHNDLNNLKAMYEALEHENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPTLKGGE"
     gene            57872..58009
                     /locus_tag="IV257_000824"
     CDS             57872..58009
                     /locus_tag="IV257_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8813972.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            58044..58286
                     /locus_tag="IV257_000825"
     CDS             58044..58286
                     /locus_tag="IV257_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8813973.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            58302..58499
                     /locus_tag="IV257_000826"
     CDS             58302..58499
                     /locus_tag="IV257_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8813974.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            58510..>59072
                     /locus_tag="IV257_000827"
     CDS             58510..>59072
                     /locus_tag="IV257_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8813975.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLA"
BASE COUNT        20454 a         9628 c        12153 g        16837 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt
     3481 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
     3541 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
     3601 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa
     3661 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
     3721 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
     3781 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
     3841 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
     3901 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
     3961 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
     4021 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
     4081 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
     4141 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
     4201 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga
     4261 tttgaactca tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt
     4321 cgttgcttca cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat
     4381 tagtttacgt gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt
     4441 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg
     4501 gcagagaaaa cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat
     4561 ctgagtgcgt gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca
     4621 ataggaagaa gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc
     4681 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
     4741 tctagtatga ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat
     4801 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
     4861 ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg
     4921 aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga
     4981 taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata
     5041 aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc
     5101 gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa
     5161 aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg
     5221 aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag
     5281 tagaatccta taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca
     5341 tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa
     5401 aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag
     5461 atgacattgt tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag
     5521 ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa
     5581 taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag
     5641 acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag
     5701 aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca
     5761 tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga
     5821 tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt
     5881 acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc
     5941 ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata
     6001 aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac
     6061 aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga
     6121 tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc
     6181 aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg
     6241 cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat
     6301 atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt
     6361 acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc
     6421 tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc
     6481 aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc
     6541 aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat
     6601 atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa
     6661 tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     6721 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     6781 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     6841 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     6901 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     6961 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     7021 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     7081 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     7141 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     7201 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     7261 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     7321 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     7381 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     7441 agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt
     7501 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     7561 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     7621 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg
     7681 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt
     7741 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     7801 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     7861 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     7921 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     7981 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     8041 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     8101 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     8161 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     8221 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     8281 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     8341 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     8401 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     8461 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     8521 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     8581 tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc
     8641 atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc
     8701 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     8761 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     8821 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg
     8881 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     8941 gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     9001 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     9061 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     9121 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     9181 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     9241 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     9301 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     9361 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     9421 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     9481 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     9541 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     9601 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     9661 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     9721 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     9781 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     9841 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     9901 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     9961 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
    10021 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
    10081 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
    10141 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
    10201 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
    10261 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
    10321 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
    10381 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
    10441 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
    10501 gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg
    10561 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
    10621 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
    10681 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
    10741 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
    10801 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat
    10861 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
    10921 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
    10981 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
    11041 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
    11101 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
    11161 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
    11221 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
    11281 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
    11341 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
    11401 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
    11461 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
    11521 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
    11581 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
    11641 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
    11701 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
    11761 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
    11821 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
    11881 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa
    11941 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
    12001 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
    12061 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
    12121 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
    12181 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
    12241 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
    12301 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
    12361 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    12421 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    12481 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    12541 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    12601 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    12661 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa
    12721 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    12781 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    12841 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    12901 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    12961 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    13021 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    13081 gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt
    13141 tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag
    13201 gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa
    13261 aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga
    13321 atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag
    13381 atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg
    13441 gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc
    13501 agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag
    13561 cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac
    13621 gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt
    13681 tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac
    13741 gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca
    13801 attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa
    13861 aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat
    13921 gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct
    13981 acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa
    14041 acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt
    14101 tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt
    14161 tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct
    14221 tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa
    14281 actatctata ataatcttac aaaagataag taactggagc aaataaaaac acttactgac
    14341 aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta
    14401 ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa
    14461 aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag
    14521 ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag
    14581 ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt
    14641 tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag
    14701 gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac
    14761 accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt
    14821 agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt
    14881 agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat
    14941 cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat
    15001 caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt
    15061 gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt
    15121 atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga
    15181 gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc
    15241 aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa
    15301 aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga
    15361 aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg
    15421 taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta
    15481 ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa
    15541 cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag
    15601 ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca
    15661 cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc
    15721 gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa
    15781 tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg
    15841 ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg
    15901 cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga
    15961 ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa
    16021 tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag
    16081 aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg
    16141 actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt
    16201 gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta
    16261 ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat
    16321 tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat
    16381 ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac
    16441 catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa
    16501 atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata
    16561 ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt
    16621 gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag
    16681 aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt
    16741 agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga
    16801 ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat
    16861 ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc
    16921 tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt
    16981 catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt
    17041 ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc
    17101 aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta
    17161 ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa
    17221 agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga
    17281 atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc
    17341 tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga
    17401 agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta
    17461 tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa
    17521 tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca
    17581 attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt
    17641 tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg
    17701 tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg
    17761 tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt
    17821 acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga
    17881 aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc aagggttctt
    17941 tttttattat ctgttttttt atattaacga tttctatttg gtacattttt gattaataaa
    18001 aaggggcaaa aaaggggcaa caagttacag tttttctata tcttctttca atttttcttt
    18061 tactcctttt gtcacatgca aataaatctt ttcagtgata tcactatttt cgtgcccaac
    18121 gcgatcctga atagcataca gaggagttcc tagttccgct aattttgaaa tatgggtatg
    18181 tctaaaaata tgtgaactaa gttttttatc aatcttcatg tcagctttat gatttctcaa
    18241 ataggtgtta attgctgtta gttggaaagg agttcctttc gttgtttgaa atagaaattg
    18301 acccttcgga tttaattcta gaagctcatt gtaaatagct atcgcttttt ttggtaaatc
    18361 aatttctcgc attccagcag cagtttttgt agaatcagat tttttcatat cagctattga
    18421 acgttctcta tacatcatcg tcccatttat aactactgat gcattattat tggtaatgtg
    18481 tacatcgtct ttacttaatg cgattgcttc tccaggtctc attccagtca aatatagcca
    18541 ctggaaaagg agagaatatc ttttattgtg tgaagttgtg aaatctacta gtcttttata
    18601 ttcatcatct tctaaaaact tatctttaat ttttatcgtt tttgactctc gtttataatc
    18661 aataactact tcgttgatag gatttttttc tacatagcct tttttcatag cgtatgaaaa
    18721 aagaagattc aatttggatt taattacact gacgtactta tttgacaagt catctttata
    18781 tatcatatct tcaaaaatat tatttaaatc tactgtatta ataccagaaa cgatgtaagt
    18841 ctcaggcatc ttcttcttta tagtgtttaa gatattattc gtaggataat atgtagactc
    18901 cttaacttgc cttttgtaaa taactaacca ttcttcgaca agctcatgaa atgttagatt
    18961 aggcttctgg agtgtttttt catttagttt tatatcaatt tttttattta actccagtaa
    19021 tgccactttc tgagtttctc gagacttatt tttataagta atgctcactt tttttctttt
    19081 tctagttttt ggatcaatat atcgttcatt atatttatat actttttttc cgtttttatc
    19141 agttttggtt tcaatccaca ttgttttcat ctcctatttg ttgctataat aggaatggat
    19201 aagtaagccg attatagcag gtttattttt catcacgtcc acaaactttg gcgagggagg
    19261 gggcgtgttt ttgtatttta acgatttata ttgaatgttg attcagcact ttcagcaact
    19321 gtactataat cagacggatt atataacact ccggttttta tcgttacttt tgtaatatcg
    19381 ttaactttag atggatctaa gtaagctaaa ctataaaatt ctttactttc agtttttttg
    19441 aaaaacgagt ttgttgtcat tgtatgcata agatcgtctg aactgaaatc aatttgttgg
    19501 ccgttatcaa gtatggcgtg tgcaagccca ttataaccat aatctacttc agaattgttt
    19561 ttaactttga atgcaatttg taaaccataa aaatttcctt cactatcttt gtgattattt
    19621 gcatcgtagt aaccagcagc gctagggtca gcataattgg aatacttgag tagtttgata
    19681 ttagatatat aaatgccgat atcaccaaga ggaatatctg attgtgggct tgccgttcca
    19741 attaattcaa ttttgccact tgtatctctt ttttctttgg tccattgacc aactttgagg
    19801 agggggccat caacctcctg tgaattttta gaagactgtg ttgtactttc tgataacgta
    19861 actgaagagt ctttttcgtt tgttttgcca gtattatcta caacattaga ttggctacat
    19921 ccggtaagaa aaaaagcacc aaatactaac cctaaaatca cttttttcat tctttattct
    19981 ccgtttctat gatattattt ttatgtagga tcttagaaac gagatcttag tccgtgttgc
    20041 agcacgggct tttttcttta taactttttt agagattata ggcaaaatag taggacacaa
    20101 aaatatatta ttgtattccg tatttagaaa atcctaattg aacttcacca gaagtttttt
    20161 gctgtgttgt acgcaatgct tcttcttcag acattccatt ctgtactttc catgcaacag
    20221 gcgacatccc gtatttgtta acaaaatcag taagtgataa agtgtcagcg tcttgctgac
    20281 cgcttgtttg ttggtcttct ggattttgtt gagatgctgc ttgttgttct ttctgatctt
    20341 gactgataat attgccagca tcatctgtag tcaatccatt ttcataaagg gccacgccga
    20401 aagcttccca ctctttgttg gaccaatttg cacgatcagc tggagttgac tgtaaagtgc
    20461 gttgtttcat ctgttcatat gtttcttctt gaggtgcggt ttggattgta tcctgactgg
    20521 agcttataac tgttgggcta ggttccgccg tagcttggtt agagcttgta actgttgaac
    20581 tagaatctgt cttagatgta gatttgctag tagaggaact ggtttcagtt gtttcttttg
    20641 ttttacttac ttttgtttct tggttagaag tggcatctgt tgattcagct tttttattat
    20701 ttgaacaagc tgaaagtagc agagcagtac ttaacaacaa cataacgcta acttttttca
    20761 ttttataatt cctctttctc gttgtaatat gtgtgctaac acgggctttt ttatataaga
    20821 aaacgataag cgctttctgg aagtccgtaa agattcttta attcatcgat ttttttggga
    20881 tattgatcat tatcttcttt ataaagagaa acaatgagat tagcagcaaa gcaattagct
    20941 tcgctttcag atttgcttct agatgttctt gttgatacat agtaactgga taagccgcga
    21001 tgaaaaatag cgtgacctaa ttcgtgagcg caaatgtaga atctttcctc agagtctcgc
    21061 agttcatcat ttaagaagat tattgcacga cctctaattt cttgaaactg tcctttggga
    21121 ttttcgataa aaggaacgta ttgaatttta atgcccatct tttcacaaat ataaaaagga
    21181 ttagcagacc ggtatttccg cttcaactcc tcgactaaat taatcgtatc catctccata
    21241 agctcacatc tttttgcctt tttctttgtc ttctttcaca atatcccaga aggttccaat
    21301 aaggatatct tttacgcgtt ggacctgttc aggcgtcagt gtttctccgc cataagacat
    21361 attgacattt gaatctagta atttatcaag ttcaactact tcatcttttg ttgcccagtc
    21421 aggaacttgg tttctaccga gtaaataatc agtagttaca ttaaaataat ctgctacaag
    21481 ttgtagtctt ttagtactag gagttctatt tttccattga taaatcgtgt ttttaggtat
    21541 atttaattct tcttccaatt gagttatact tatgcctctt ttatgagcta attcctttat
    21601 tctatctagt aaattcattt tcgcacctca caagctacga aaacactaat aaaaaaatta
    21661 gttaaaggtg ttgacaacta ataaaattgt tagtatactg ttttcgtaag ctaaaatatt
    21721 agctaaaaag acttgataac taataaacac ttcacggtcg gcaaactctg aaatgtaaat
    21781 tactaggcat ttctgtgtct tatttagcta tatctatata ctaataaaaa tattagttat
    21841 tgtcaacgaa tattagctaa ttttttagct tacagactat tttttagaaa ggagctattt
    21901 ttatgtctga gaatttagac ttaaaaattc gagcggagat gagaaaaaga agaatgactt
    21961 tcaaagaact agctgcgctt gttggtattt caggagctta tttatcagat attctaaacg
    22021 gcaatcgtga tggaaagaaa gcacaacagc atatcgaaac agtgaaaaaa atattggaca
    22081 tccgataggg ggggtaggcg atgacaaaac taaaaaaaca agattttgta aaaaaataca
    22141 attattctcc atctacttat caacgtcgaa tgtcggaact aaaaaataca gcaattttct
    22201 cagcggcgta tgaacgggtc acaggacaag aagtttggat caatacagaa ttatacgata
    22261 aatttttgtc tttcaaatcc tataacaggt tacgcacaag aaagataaca cctaaagaat
    22321 ttatcgagaa gcatttagtt gatttataaa aaacagaaca ttttgagagg tgaaggttaa
    22381 tgggcaaatt caacagagca ctagtattca gcacaccatt catcatctac gctttaggcc
    22441 tctggggaag cagacaagcg ttgataggaa cgattgttta catggtttgg atttttatgg
    22501 ggctggatga agctgagtac aaaacaaaaa agccagtcgg gagggactga ctaatgaaaa
    22561 aaagtttaat gactataaac gaaaaacaat tgaaagaaaa ttttaatgat ctcatcaaag
    22621 aatttgtaaa agaaactgga gaatttccta atcaaattca tctagttgcg gaggggcata
    22681 gccagtatca agctgtgaaa tttgagatga agaaacaaat ctattgaatt tagcagtacc
    22741 tctacttgta agaacattta ttgtaatgtt tttttcgact aaagattcat cgtatctaaa
    22801 aaccaacgta gtaatttttg actcatacgg tggaatggta ataggtaatg acaacggagg
    22861 aaattttgat agatcagcac ctttttttaa agatttacca gaatacgcac gtataccatg
    22921 tggaagttcg tcatacattg attttaccgt tacagaataa ttatcaccaa tcattgtaaa
    22981 agcatttaaa tgatcaggaa ttccagaaat cgaaaactca ataattgtaa caggtaaaga
    23041 actgttattt gttaacaata cagaatctat aatcctgaat ttattttgat ggtaaacatc
    23101 tggtttggaa tcactttctc tatctatcaa ccattcttcg cattcagcga agaaatccaa
    23161 ttttaaatta aattttttgt tttttctatc tgtataggag atataaaaag caaatgttga
    23221 aaatactaga gacgtaatag gcaaaataaa atctttaatg gttttaacaa tgttattcca
    23281 gtcaaattgt ttaataaagt caataagact caatttatac caccaccatt ttttatctac
    23341 attatatcaa agaggagaga agaaataatg caagaattag taattttgaa aaataaagaa
    23401 gctgtgacta cgagtttgca agttgcagaa agttttgaga aaaagcatca acatgtttta
    23461 agagatattg atgcactgaa aaaagatgtg tccaattttg gacagatgtt ttctgaaggt
    23521 aatgaaccag attcatacgg cagaaatcga cgagttttct tcattagtag agatggtttt
    23581 ttcttgttgg ctatgggttt tacaggaaag aaagctattt acttcaaaca aaaatacatt
    23641 gaagcattca acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa
    23701 gatatgatga tctaccaact aaaagaaatg aaagatgtta aaaaagatgt ttccatgctt
    23761 aaagatacta tgcgaattag tgggcaacaa gagtttgaaa ttaaacaaaa aggaaatata
    23821 aaagttatgg aagttctagg gggtaaagaa agccgagctt atgaagaaat cagcaaaaaa
    23881 gtattctcaa aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag
    23941 ttacctcgta agagattcga tgatgctgtt tcatttattg aaatgtggtt accagaaact
    24001 gcgatccgca tggaaatcga tcaactgaac agacaacaaa gacttttcgg tgatgaaaat
    24061 gaatagagct gaagcgctaa gaatcgggac ggtaattgct aatcgctggt ggagacacaa
    24121 taaaccaatc atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa
    24181 aaagtgactc cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag
    24241 catatggaaa aagaactttc cactctagat caatatttga ctgatcctag ttggggcaaa
    24301 tcgaatatca aggaaacaaa taatcgaaaa atcagacgaa atcttttgac ggatgaagaa
    24361 ctagcatgtg atcaagacga tttgggaaat tttgtgagta tttgggatca tgtctatctt
    24421 atccatttat cgaggaagtc caaaaaacct gaatatatct atgtcatcga agatggcttg
    24481 attgatgcgc tagaagagta tgacagagat aacttgattg atatctctta ttacggatca
    24541 ggtaagaaat acattgctga aatggaggca gaatttgatg agtgaaaaca aagggacaac
    24601 gacttttgaa aaacttttta gtcgcaagtt aaataaaata ctcaagaaaa aaggaaattt
    24661 tgattattta tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac
    24721 ggtaactatt aatgagtata aacactacag ggttgtttct ggaactcatc aagactttct
    24781 tgttgaggaa tataaacctt ttcttatgga tgaaacaggg acttatgtat ctgtctcagt
    24841 aacggttaaa ggacacacgg aaaccgagtt atttcctgtt ttagattatc gaaaccaacc
    24901 agttgttaaa ccaaatgcaa tgcaaatcaa taactcattg aagcgatgct ttgtgaaagc
    24961 attggctcta cacggactgg gattatatgt atttcaaggg gaagatattc caacaccacc
    25021 tagaatcgat acgaagaaat taaacatgct agagacgatt ctagaagctt tcaatgagca
    25081 gatgggtaaa gatatgacca aaaccttaat cgaatatgtt aatgagcaga cagataaatt
    25141 agggctctta gctgataacg ttgaaactat tgaacagtta agctatgagc aatgtgcctt
    25201 gatggagcga gcaatagcag ctaagagaaa agaattagat aagaagtgat atgagtgttt
    25261 aaaccattaa tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa agacataggt
    25321 gcaaccatca atgaggaagt gaacattgat cgactaaaga cgatgtatga cggctacgat
    25381 ggtgatcgag ttattgaaat tcgttttatt gatcctagac gtttcacagt acagcaacga
    25441 aacttcatct atgcactcat aggcgatatt ttcatcgata caggcatgcc aacggacttc
    25501 tggaaggaat tcttttactt ccgtttcgaa ggtgtcacag ggcgcgaaat aagcctcaaa
    25561 gatgaatcga atacgactgt gagtgatgcc aatatcttag caaatatcat cttagatttt
    25621 atctttgaac atcatattcc tttcaaagaa ggctatgaga ttttaccagc gaatcaagaa
    25681 tattacttct acaaatgcat cacaaaaaga gtctgctgta tctgtggcaa aacaggagct
    25741 gatatcgatc actttgacaa agcgctagga agacgaaagc gcaaagaagt tgatcattca
    25801 gagtacacat ttgcagcact ctgtagaatt catcacacgg agaaacacaa aataggtgtg
    25861 accaatttca aaaataagta tcaaatcaaa gggattaagt taaaccagga gacaatcaag
    25921 aaattaagaa tcgggggcta atgatgatta atttaaacgc agtagcgttt gaacaaacat
    25981 ggcgcactaa gtataaaaaa atgagtccaa gagacaaatt gttcttagaa atcatgacat
    26041 ttgctttcat tggtacacaa gctgaacaaa gcgatattag tgttgaaaaa attaagacga
    26101 atagattagt taatggaatt acagagactt gttaccagta cacgattatc gtcgtggatg
    26161 aggaggaata attttggcag aaagaagaat gttcgcaaag acaattattg attctgatgc
    26221 attcctagat atgcctttat cgacacaggc attatatttc catctatcaa tgcgcgcaga
    26281 tgatgatgga tttattaata atcccaaaaa aatacaacgt atggttggtt gtggtgatga
    26341 tgatttaaaa ctgcttatgg caaaaagatt catcttagtt ttcgagagcg gtgtgatcgt
    26401 cattaagcat tggaaaatac acaactatat tcgtaatgat cgttacaaac ccacgttata
    26461 tcaggatgaa aaagctttac tagcagacaa agataacaaa gcttatacat ttgcagaaga
    26521 gctttctaaa catgatgaaa aacttggtat accagatgac aaccaagcgg tataccaaat
    26581 ggatacacag gttaggttag gtaaggatag gttaggtaag gatagtaaag agataaaaga
    26641 tttaacgcct tcgaaaaaat cgaaggctaa gcccatccgt cataaatacg gagagtataa
    26701 aaatgttctt ttgtcagatg agcagatgga gaaactcaaa acagaattcc ctaatgatta
    26761 ccaagagcga atagaacggc tatctgagta ttgtgaatca tctggtaaga cttataaaaa
    26821 ctatttggca actattcgaa gctgggcaag aaaagaaaaa agtgaatcta agaacgcaag
    26881 cagtggatac aagcgcacag gaagacgaga gaagcttcct gaatgggcaa tcgaccaaga
    26941 agcctatctt aagaaaaaag cgctagaacg agctaataga caatcaaaag caccatttta
    27001 agaggtggaa aattgaaaat cgattatcta gaactaatta atgaaatagc gaattataaa
    27061 actggcgagg aaatagacat cctgagagac gtatatgatc aactcgaaga agctggaatc
    27121 gaaggaatta ataatgatcg ttcgagttgg agtaaactca gatactattt cgcactttat
    27181 atcgatgcaa cacaattaag aaatttagct tatacaaaat tactatttgt tgattgcatt
    27241 aaaggattgc aaaaacatct tagtgaactt gagcaggtgt aatcagatgg atctaaaaac
    27301 atttacagca cagatcgaac taatgcatca agaagcttta agacaaagcg cctcgtacga
    27361 agacaagtgg ctcaacacgt tccatggtgg acgtgagagc gcacttgatc aagtactcaa
    27421 attattgaaa ggggaacgtc gggatggata agaaagcggc aatgaaacga atcatcgaac
    27481 tgacacattc tgagaattgg caagaagaca aagaaatagt tacagaagtc caaaagctcg
    27541 gtaaatcaat gtggactgaa aagcccaaac ggagaacgcc gagaaagatt gcaatttggc
    27601 atggtgatcg aattctagta acaggtactg ctgaacagtt atctgaaatt actggtctga
    27661 gcaaaaacat catctgggat agagcaagga gcttatggat tgattcaaaa ggacgacaat
    27721 ttaggtatgt ggaggagaaa aaatgaacga actaatcaca aaagtagagc agtgggctaa
    27781 agacaaggga ctggatcaag cagatccaaa agcacagttt ttgaaagtag ctgaggaatt
    27841 cggggaaatc gcttcagcaa tggcaagaag taatgatgag ctatttaaag atagcgtagg
    27901 agacgtaatc gtcacgttga ttatcctttc catgcaaaaa gggacaaacg tacaagagtg
    27961 tttagaaatg gcatacaacg aaatcaaagg gcgcacagga aaaatggtag atggtgtatt
    28021 cgtgaagtcg agtgatttgg aggacagcaa atgataggtt taacaatatt ttggtgtgcg
    28081 attggtggtt tactagcatt tctaggatac tacctcatct gtgaaagtag aaaaggaaga
    28141 aaatatacag tgacaggtat tgtcctgtta attatcggaa ttctagttgc agttggagca
    28201 gaactgaatc tttatgtaaa tggtacacaa gaagatttgg tgcgatttat gttttggatg
    28261 aagaattagg aggaaaaaaa tgaacgaaaa caaattaatc aaattaggtg tagcaggagc
    28321 agtaatcgta ggtattggag ttatcggagg atttaagttc ttcgaaaaaa tcgataatgg
    28381 atatgtgggt gtgcgctatt caatgaacgg cggtatcaaa gatgaagcac tgacgcaagg
    28441 tgtgaaattt gtagggattg acaaagtgat ccaatatcca attcgcttgc aaactatcca
    28501 atcaaaaaat atttcagtat ctacaagcga cggcaaaaag acaacgattg atatcaaata
    28561 tgactacaaa gttgattcaa ctaaagcagc aaaaatgtac aaggaatttg ggaatatcac
    28621 ttcggaagat atcgaaagtg gatggttaaa atctaagctt caaaaggtcg ctcgtgaagt
    28681 ttatgcgaaa tatagtctgc ttgatgtcct ttcaggagat tcctctaaag ttgaagctga
    28741 ggtattaacg aactttgcta aatcagttga atctaaaggg tttgaagtcg aagacgtaac
    28801 acttggtgtt ccagatgtcg ataaagaaac acaaaaatca atcgatgcga tcattcgagc
    28861 tggtcaagaa aatgaaaaag cgaagctaga tgcagaaact gcaaaaactc aagctgatag
    28921 tgaagcttac aagaaaacaa aagctgcaga agcagaggca gaatctaatc gcaaagtcgc
    28981 cgaatcagta acagacaatt tgattcgtta tgaagaagct caagctcgca aaaagcatgg
    29041 atgggtaaca gtaaatggag cagatactgt agttacggat gaagcaggca aataatatgg
    29101 gattctttat ggctaaaatt ctcttgttct taggtttagt aggagcagca tatctcgtgt
    29161 atgcgctcct ttcccaaact gatgacaaag aagatgacaa caacgatgaa atgaaatttt
    29221 aggaggagaa ataatggatc tcattacaca atacagtgat atcatcctca agaaaatcat
    29281 ggcaaagatt cagaaagata aaaaagcaaa agaacgagcg gaattagtta agttggaaat
    29341 ggctgaaaca ggagtaggag tgcgaagtag caggcattgg aaagcagcag caaacattga
    29401 attttattac aacgaaattc aaaaagggtt cgatcagatg cgtgagctgg atcggcaaac
    29461 aaattggagc aagaaacttc atcaagatcg tttcaaattt gtagagaagt ataaagaaat
    29521 attagaagag tatttgagga ggacagcgaa tgataaaaaa gctcattcaa ttcagcatgg
    29581 atttatatga tatcgaatca ggagcaacag tatctgtgga gtctgatcat ttaatcataa
    29641 gttttgctga cgaacgtcaa attatcatat gggtagttga cgatatgcta tatccagaaa
    29701 ttgtacatga ttttgaagaa tcaaaagcgg ttgagtttga aatagtgaaa aaagtaatgg
    29761 aattgattga aaaatacgag gaggacagca aatgataccg aagtttagag cgtgggataa
    29821 gcgagaaaac acaatgagag atgtagctgt cttacatttt actaaaaacg gcaaaacaaa
    29881 ctttattgaa tattggataa atcctaccga attgaaatca tatcacgtgc gaaacatcga
    29941 cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg gagatatttg aaggggatgt
    30001 agtaattaca tcaaaaggag cgattggata tgttgcgtat cttcaacaag aagccggctt
    30061 tgtagttgta ctaaagaaaa gtgattatag attaggccat aggaatactg gcgaaagtta
    30121 tgatgtagca actgctcatg aagtcatcgg aaatatatac gagaatagcg agttattgga
    30181 ggtagagtga ttatcttagc tactgactac atcgattctt taaaagatga aaatgggaat
    30241 gtcccagcat tgctaaattg tggaattaaa agcaagaata gaatcatttt gttacggata
    30301 tcggctgatt tagcaaaata tctttatctg agcaagacag cagtatatta cactgcggct
    30361 acacgttatc agtacaaggg aaaaacaatc agtcaggatt attatgatcg tttaatcagt
    30421 cctgacatgc atggaaaatc aaaatcagcg atcaagttat ttggcgcaat agagatatat
    30481 gccgatgatt ttccaaattt atggttgaag gaggaagcgg aatgaaatac gaaataccac
    30541 taagtgaagc gggcattcaa gcaattatca atggtcggga ggttaataaa aaacttcctg
    30601 atggtactga attagtcatc agacaaagtt atttgaaaga tatggcagct ccagtattaa
    30661 ttgatcgttt taacgtgact gattctgtgg tagagaacca cttgaaagaa tttcgatcaa
    30721 gtataaacga cactttcaga ttagggagtt gattgacaat gaacaccaga caccgcagag
    30781 tagcaaaact aagaaaacag gaactgaatg tactaaagac aaagtttgaa aaagaatatg
    30841 gaatttcagc agaagaaaca tataaagtgg caagtcagtt tgttgctgat gcgagtgaga
    30901 ctattcgtaa gtttgggatt tcgatattaa atgatgatcg taaatgggag gcagaaagat
    30961 gaaactaaaa gacggatttt acactgatgc ttatggaatt ggtggtttga tgatggatct
    31021 accaactaag aatcctataa agcaagagga atcagaaatt aaagtcggtg acatggttcg
    31081 ctgcgaagca gaagggttca tctatccatt tcgtggatat gtagagcatc tctataatca
    31141 ctcagcgatc attcgcattg aaaacacgat ggaatgtgac aagcggttag cgaaaagcaa
    31201 agagaattta gcagtggtga gattggtgga tatggaagtt ataaaatctt aattttgctt
    31261 ttttacaact atcctacaac aatgagcgtt attccacaac tggaaggaga agaggcaatt
    31321 gtgaacattt tagagaatat agatattaag caaacaagga aaaatgctag acgattacta
    31381 aaaagataca gaaatttaga acgtttggtc ggaccagtga aaatagactt ttccgtgatg
    31441 actgttacca aaaatttgaa attcacaatt gacagtcaaa acgaagaaat cattgaagcg
    31501 ataagtactc gagattcggt tatcgaagca ttaacacggc taagtagaat ccatttccag
    31561 gtactttatt atagttattg ttttcctaat aagatgtcaa tgtatcaaat aggagaaaaa
    31621 ttaggatatt ccgatagaac tatcgagaga atgaaggcag tagctttggt tgaatttgct
    31681 gaggcgtata aatcaggaga actcatttca cgtacaaaat aaaaaaagtc ggattcctcc
    31741 gactatgaat aatatttccg acataagtat tatatcataa ttgggggaat cagaggatgg
    31801 tacttttcga tgtaaagaaa tatgagacac caagcgcgaa agatgttgat atggaacgca
    31861 caaaacataa tgtcgctgtg tttctttcag catatctatc agctagatgt agagtagggc
    31921 aacctcgaga accaaaagtg acagcatctt attccttggt tccaccttct acagctgatc
    31981 atatatttga agcagaaaga atgctgatcg ataaagaaga agcacaggaa gaatttgagt
    32041 atctgcataa attgtttgtt cgaggctatt cagcgataca gcatccgcat aagcctgatg
    32101 tgactgaaag gcgcaagaag atattctatg atcgttatat caatggtctg cccatttatg
    32161 taactgctca aaggaataat actagcgaag aatcggttaa agtagaatca aacagaatta
    32221 tcatccaatt tgcttcatcg ttagaactgg ttgctttcaa gtagccagtt tttacacttt
    32281 ttataccctt ttattaccaa tttggtttcc attttatacc ttttttatac caatcactta
    32341 cctattcaac gttgtattat gatagtgtcg aaagattagt gataggtctt agacaaaata
    32401 aaatgtaagg gaggaaatct ccctcatcgt tttaaattaa gcctcgatag acagcagcgg
    32461 aaatattaag aataaggatg tgaactcaac tccttctaaa ttgttcttat tatctatcat
    32521 ccgttgctgt ctattaattt atgtattgga gggtatataa aatgaataac gcttccgaaa
    32581 aaattgatca actaactatt ttagtaaacc aaagaatcga agatattatc aaggaattcg
    32641 aaggtgaaaa gattaagagc ataaagctta ctttaatgga tcataagttc gaactaaatt
    32701 cgacaaaaag gaagatcgtt gagcttatag aaaaagaaat tgctgtccag atcaatgagc
    32761 aacaattaac taattaattt atgcatcgga ggaaaaacga atggataaag aaagtgcacg
    32821 agaactttat ttgagtaaat tcaaacgtaa caacaaacca gaagaagttt ttggatcaaa
    32881 cgttaaagag gttggagaaa aactaactca attattaaaa gaagaagact taacatatga
    32941 cgaagcatac gcaagtcttc agtactgtta caatctattg aagtatgagt ctaattttct
    33001 gaaacttcaa tagtaaataa ttcgtaattg gattctggca acaaacaaaa attgaatttt
    33061 tttccatcaa tatattccgt attgtaagcc aatgtagaaa attctctata gtcttctatc
    33121 tgcagattgc ctataaaagc tttaattttt tctaataata gttggggatg atccaaaagt
    33181 tttactgatt gtctaggata tgaaaaacta tcattccatg tcttagcagc ttttgcccaa
    33241 aggaatcctt tttccgcacc acttgctatg gatatctttg ggtttgaaaa gtcaaaatct
    33301 tcatccaaac tttcttgaat cacagaccaa cctgtttcag agagttttaa aaagtctgca
    33361 ctgaaaattt tgcaaccatt cttaacatac agtttaacag tataattatc agggtctaaa
    33421 caatttaaaa taaaatacgc agcttgaacc tcgcttaagt tcatattttt caccacctat
    33481 aatttatttc agcggaccac tcgctgataa ctaaaattat acgcttagta tttattttca
    33541 caatattaat ttgtcactgt ggcggaaagg gtagacgcta tatctggtag gtatgcttga
    33601 agaccagaga gacaagagct aagcgcaacc atgcaaggtt cgattccttg ccagcgacat
    33661 taaatgccta tgacggttac gactaccgaa aaaagatcgt taagaagcta tacggtgcta
    33721 cgtacggcaa tgtagtaagt gtgctatctg tacaccacca agcttcggtc actgtggcgg
    33781 aagtagaaga cgcagcggta aatggcgagt agcctcgtga gagcctggta agttctcgtg
    33841 agtggtgcaa tcccactcca gcgactttaa gcaaccgagg catcggcggt ttaaaaatat
    33901 aggggtgcgc aatttcgtac gcgttttgtg catcgtgtag gttgctatta catattagat
    33961 cactcatcga gtgatctttt tattttgaaa ggggagtaaa caaatgaacg aaaaccaatt
    34021 aagagagttg tttaaaacga atgaagcaaa caaaacaatg gaggcgacat tctacgaaac
    34081 tcaaaaaagc ttagcgttaa tcgcaaaaca atcaaagtat ttctacgatc agcttattct
    34141 gcaaggattt aatgaaggac aggctatgga atttatgatg cgaacctttt ctgccagtaa
    34201 ccaacagaaa gagtgataca taatgagaaa ctactggtat atatcgctaa ctaatgaata
    34261 tcctcgaacc attgatgatt gttcagtgcg tgttgtgcgt tctgtacaaa tcaaaaagaa
    34321 gtactccatc attgaaatga ccagagaagc tacaccaaat gagatcgata agtacaatct
    34381 tcgttactgt ggccatggat attggaaaga cgaatatatt caacaaaaca ttgagaggta
    34441 catcaaatga atgataacta tgattacatc aagttgattg aaaagataag agcagaaaaa
    34501 gatatggacg aactagctaa cttattcatg aacatcatta gtttggtggg gttaaagatg
    34561 gatgaagtag cggcactaaa ttatttcatc gctgagcaaa caattagagc agagcacaat
    34621 gctaagttct tgaaagatag gttagatcta gatgtaaaag gattaggcgt tgaagggata
    34681 ttcaaagtgc aagaagcctt agtgaatgtt tatgtcgaaa agatgcaata gcattaacaa
    34741 gtaattattg gatgttttca ggagggaaac tatgaaatta aaagattata tcagggaagg
    34801 gtataacatt gtaactacac caaatctagc ttacaaaatt caagaagatt atcccaatgt
    34861 tttagtgttt actgatagag ctttagatgc cattcctata gggaagttat tagtagatcg
    34921 ttattatagc aacaatccag ctgttcttag tgctaagcca cttcaaaacg cttatgctat
    34981 tgaacgcttt acttgcgagt ttactggata tgagacggta gtaccaatga caggcgagac
    35041 aggaagaaaa gtaattcaac agatcaaaaa gaggattgct gaattgacgt tagatgatgg
    35101 aagtaatacg aaccttctag aaatcaaact aagagatact gactcagtac cagaagttta
    35161 ttataaaggt caacgaatgg atgagatgcc gataggtctt gtcgatatta cttatcattg
    35221 gcatacggaa gaatatgaat gccaagtacc aaaaggagct aacgacatca ctattgaata
    35281 ttatgattca cataataaca agtacttaga tagaaaaatc attgggcaca agagagatat
    35341 gtaaatgatt gatgtttcaa ctaaacaagc aagagctagg ttctatggat caactgagtg
    35401 gaggaaactt agaagtcaat gcttagagcg agatcattat gagtgccaat ggtgtaaagc
    35461 agagggtaga ctaacaactc aacatgattc aattcttgaa gtggatcata taaaggagct
    35521 ggagtatcat ccggatttag ctctagatat aaacaaccta agaaccttat gcaaagattg
    35581 ccataacaaa aggcacaacc gtatgaaata tcgtggccaa caaaagaaaa gaaagtggga
    35641 tgatgaatgg tggtaagaaa aacagcaaac agagatgatg tcaaacgagc agaagcaatc
    35701 gctatggttg ataaggctat tgataagttg gttctattta agagtcagct cattgaagaa
    35761 acaacaactg tagaggatat atctatcaac aatagactaa taggtaacgc taatgaagta
    35821 acagggacat ttaatattaa tgtagacatt gattatacct tgagtccatt tgcacatatt
    35881 gtagaaagca acgagcaaga cggctgataa atcagtataa agcgaagata tatagagtga
    35941 aatacccccg gtcaaaagat tttgagaaga aataaaattt ttgggaaccg gtggatgggg
    36001 ttaattctgc aaatgcaacg tatttatttt acccctcccc cctccgccgc ccctttagta
    36061 aatatagaag gaaggtgatt ggtttgaatt ctaagttggt tggcacgaga atgaaacaaa
    36121 aaagaattga aaaaggattg acacaggctg atgtagcaac gaaaagtggt atttctagta
    36181 ggtattatgg aagtattgaa aatgggaaaa attcccctag tatcgaaacg ttaaactcaa
    36241 tttccatcgt cattggatgc agtttacatt tcttactcaa tgatcgcatg gacgacatgg
    36301 aaaaacgcgt agagctagaa aagaaacgtc tgcagaagat attcgataaa attcctcatg
    36361 acaaacttaa tttagttgaa gggctgatca ttcaggcagc aaggttacgt gtcttgttgg
    36421 atgataactg gaaggacatc attgaaaacg gggagtacga gaaatttaaa caaagcgaga
    36481 accaaattgc ttatgataga aagcgtccta tagttgaaaa ttatgataat cgagataaaa
    36541 cttatcaagc catcgtcaaa caactcaccg atttattacc gcaaggcaca agagcggaca
    36601 agaaaagtaa gttactcggt aggaaataaa aatgcttttt aaccagcacg ttgatcaata
    36661 tattcgtaaa tggaaatctg gtgagttaat ccttaacgaa aaaaggatac aacttttaaa
    36721 actaatagaa aaagaaatat tacctcgcga cgacatttat tacttcgacg aggagcaaat
    36781 aaataattat atcgagttta gtcaagcatg gtattttgag ttagatgaat gggagaaatt
    36841 tatttctgca ttcatttttt tgttttataa agaagatgac gaagttgttt ttgatgaatt
    36901 tgttattaat atggggcgtg gcgggggtaa aaatggcttt atttcaacat tggcaaatta
    36961 ttttgtcagt tcgcttcacg ggattgatta ctatgatgtt tcaattgtag ctaatagtga
    37021 gaaacaagca aaaagaagtt ttcaagaatg ctatcgagta atcaataaaa aaggaaatga
    37081 agatttaaaa gaagagtttg aagcgtataa aagcagtcta accggtttag agacacagtc
    37141 tgtttttgag tacaaaacta gtaatgcaag ctctcaagat ggcggtcgag aaggtgcagt
    37201 aatatacgac gaataccacg aaatggaaaa cacagatatt gtagatgtgt tttctggtgg
    37261 actggggaaa gttgatcacg gacggcaatt tttcattggt actaaggggt ttgttcgtga
    37321 aggttacttt gatattaaat atagagagtg tgaagatatt ttaaatggtt tgtcagagtt
    37381 caagggtgta tttccgtaca tatgcgaact ggatgccatt gaagaaatgg atgaccccaa
    37441 aaattggccg aaagcaaacc cggcactgca accaccgttg aataaacgtg ggaaacgtct
    37501 gtttaacgaa gtcatgaaaa agtacaaaaa gttagctaaa gaaccttctg gacgctcggc
    37561 atttgttact aaacgaatga attttcttga agacaatatg gaaaactctg ttgcttcaag
    37621 agaagaaata atggcaacaa atcgtccatt ttttgaacta gatacggtac cgattggatc
    37681 tctcgatttc ggtagtgttc gagattttgc tacttgcggt ttgctattta aaaatcgaaa
    37741 gaaatcggaa tacgcgttta aatcatttac gtttgcaatg aaaaattttt gcgacgttca
    37801 ttatggatac tcgttgaaag agcaattggt tggcagtgaa aaacgggcac caataaaaac
    37861 ttgggaaaaa atggggctga tgaaagttat tgacgagcca tctttaaatc ctaagtatat
    37921 tgttgattgg tttattcaag ctagagaaga atatggagtg agagtaattg tcgtggataa
    37981 ctataaggca gatattttag ggcctatgct tgaaaaagaa ggatttgaag tagttcgact
    38041 aaaaagacca tctagtttac atccattatt agctcctcgt atagaagatg gatttgcaaa
    38101 tcacaaattt atttttggag ataatccgtt aatgcgttgg tttactaata atgtgtacgt
    38161 aaaggaaacg caagcaggca aacaattctt aaaaaaagag gaagtcaagc gtaaaactga
    38221 tgggttccaa gcatttgtac acgcattgta caaagctaca gaattagatg aacagattga
    38281 ttacgatgat gcttttgata tgttggatga gattgaattt taaggtatac ttttctttac
    38341 aaaggggtgc tttcatgagg ataaaaggaa aaaacatatc ttgggtattt tacatgttgg
    38401 ggattttagc ttttacattt gtggcaataa aactatcaga aattattatt ccaaatgaca
    38461 gtgtagtttc aaagaatttg gttttagaaa gagctaaact aattatttca attttaggac
    38521 cagcattaac actaattgtt tttgttcgaa caattcaaac tcaattagaa tcttcaaaaa
    38581 acttaattaa agataacgtc agtaaagatt tttataattt gctaaattta ttcaaaagta
    38641 tacaggataa aatgaattct gaattattta gtacaattga aaaaagaatt aaagtaccta
    38701 taaataatat aaatacaaat ttagatgtta gagaaaataa agaaactact aaagaagtac
    38761 aggaggattt aaaagaatat agtgcatcat tgaataaagt tttaagcgaa agcgttaaaa
    38821 aaattaatga aatatttgag gataactaca aagacatagg tagttatcta aaagtattcc
    38881 ataggattct taaaatacta aataacaggt atgaatcaaa agatatttcg catgaagaat
    38941 acaaaatgta tgttggcata ttacgtagtc agattgaaga ttacgaattt tatataattc
    39001 ttataaattc tttgtttact cttcgcggaa cgggtatggg gattgagtta attgaaacat
    39061 cattctttgg tgatgaacta gatattgatt taaaacagca tttcgaacat ccagagtttt
    39121 taactgaaat cattaaggat aattttgttg aaaagaaatc gtatcgtgaa tcagtaaaaa
    39181 caagaaaaaa aaatagaaaa ctatttgaaa atagaaagat aaatgataac ttattatctc
    39241 ttttgagaat aagatacacg ttacaagatg gaaataaaat tattataagt aatggagtat
    39301 agtcctagaa atagggcttt ttattttacc ttgaaaggtg gtgaaaatat gtgagtttat
    39361 ttgatatctt taagcaatca ataaagaacg aagatccatc ggattggatt cctgactttg
    39421 tgtttggtga tgaagaatct gctagagcat atttgaaaat catggctaaa agtacagttc
    39481 ttgattttgt tgcaagaact atgtcaacgt tagaagttaa gtttaaaaat aaagatggga
    39541 catcagattg ggattacatt ctaaatgtcc gacctaattc tgatatgtca gcagctacat
    39601 tttggcaaaa atttttttat cggctcttag atgacaacga agtgttagtg atatttacag
    39661 atgataatca attgctgatt gctgatgatt ttagtcgaac agagtatgcc gtgtatgatg
    39721 atgtttttac aaatgtgatg gtgaaaaatt ttgtctttga acgttcatgg gccatgtctg
    39781 acgtcattta tatggaatat aataacgaag aattagaaag gtttacacga ggtttatttg
    39841 aagattacgc cgagttgttc ggacggattc tagaagttgc aatgcgcaat aaccaaattc
    39901 gtgggtctgt ctccattgat tcaacaggaa cggcaaatga tgaaaaagga aaagatggta
    39961 agactagatc tcaaagatta caagaatata ttgataagat ttggaactct tttaaaacta
    40021 actcagttgc aattgttccc aaagtgaaag gctttgagta tgaggaatac accaataaac
    40081 aaggcgtatc gaatcaatca ctagatgaac tgaataagat gaagtcgtct ttaattgccg
    40141 atgttgccaa cgccatagga gtacctacgg cgcttattta tggcgaaaaa gctgaactag
    40201 attcaaatat taaagctttt cgtaaattgt gtattgctcc actagttaaa aaacttcagg
    40261 acgaattggt ggcaaaaata attagtcgac aagagtacaa aaacggggaa cgtatagaag
    40321 ttaaaaaagt tctccaaaaa gatctattag agtatgctac tcaaattgac aaaataacat
    40381 ctggtggtgc gttttatatt gacgaagttc gtgatgaagt tgattatgat gagttgccaa
    40441 ataatgaagg aaaaattcgt attcgtacga aaaactatga agaaactgtg aagggaggtg
    40501 agaacgacaa tgccgaaagc taaaaaagta ccatttcagt ttactaacga aattcagaat
    40561 ggtaagcaca ttctcacctt gagtggaaat gtacagaaaa aatattggcg tgatgatgat
    40621 gtcataaacg ctaaagatat tcgtgactct ctagatactg taaccgatga tattgtaatc
    40681 aagttgaaca gcccaggcgg tgatgtgttt gaagggattg agatttacaa ttatttaaaa
    40741 gatcacccat caaatattac tgtagaagtt acagggcttg ccgcaagcgc agcaacgttt
    40801 attgtcgctg gtgccaatga tgtgattatg aatgttggga cttctttgat gattcatgaa
    40861 gcttctacat ttgcttgggg gaataaacag gacatccaaa aaacgttaaa cgcattggaa
    40921 acgatcgatg attctatctt agctatttat tcagataaga ctggccaatc tgttgataag
    40981 ttgcgtgaat ggatgaatga agaaaaatgg tttacagcag atgaagctgt caaatatgga
    41041 tttgccaatt ctgtgaagcg tgaagaacca caaaacgaac ctcaaaatat tgcagcgatg
    41101 attcaagatg ctgttgccgt tgcaatagcg aatttaaagg aacctgttac taataaaatc
    41161 gaacaagaat caaaacaaaa atcattaatc gcacgattgc gaaaaggaga ataaagatat
    41221 gttaaaaatt acagataaaa ctgcagatgc aaaaaaggca tttaatgcta tttcttcgaa
    41281 agaagatgca acacccgaac aaattaatgc tgctctagaa gaatatgtaa ctgccattgc
    41341 agaagatgcc ggcaaacagg tgcgtgctga atatgaagaa ttgaaaaatg tgacggacaa
    41401 ccgtgtatta gaggctcgtg gaattcctac actaacagta gaagaaacaa agttttataa
    41461 tgaagccgta aaatctggag gtttcgattc tgacgtagtt tggccagaaa cgattttgga
    41521 acgtgttttt gaaaatcttc aaaacgatca tcctatttta ggcctcatta actttacacc
    41581 aactgtaggt cgtgtcaaag taattagagc acgtcgtaaa ggtgttgctg tttttggacc
    41641 attgcataaa gaccttgaag gccaactaga tgctgaattt ggtgcaactg aatttgttca
    41701 acttgcgttg accgcatttt tcttaatttc taatgatacc ttagatttag gaccacgatg
    41761 gattgaccgt tttatcaatc tttctttaag tgaagcagta cgtgatgttt gggctaaaaa
    41821 aattattaca ggttctggta aagatgagcc aattggattg cttaaagatc tagatggtgc
    41881 ggtcacaagt ggtgtatatc cagataaagc tagcgcaggg acattaactt ttgcgaaaga
    41941 gaagattgtt cttgaacttg caggggtgat gaaaaaatta tctaaataca catacaaagt
    42001 agatggtgac gatgaaggtg aagtggaata ccgttctgtt gctggacgag tgtatctaat
    42061 tgttaatcct gttgattact gggatatcgt agctagagtt acatttgcaa atttaaacaa
    42121 tgtgtatggg actacgatgc catttatcgc tgttgatcat atcatcgaat ctgtagacgt
    42181 accagaaagt aaatgcattg catacgttga taaagagtat gaagctactc aatcaagagc
    42241 tgaaaaagtc tatgtatata aagaaacttt tgcaatgaaa cgcgcaactt tgtatgctgt
    42301 cgacatgctt ggtaatggct atccaacaaa taatgatgca gcacaagtat atgacttgga
    42361 ctttacagcg ggggagtaac gtcccctgca attggtgaga taacaccgac gactgatggg
    42421 gcagtcatta atctttcgta aatggggtta gagtatgaac actgaagaat ttataaaaga
    42481 atataaggcc cgatttcgta ttttccatac ttccgaagat gaggatatcg ggaaacagtt
    42541 agagagcggg tttgccgata taaaatctct aatcggcagt tttgatcctt ccacttatga
    42601 gaaaggaaaa gaattagttt atgaacgtac tcggtatcta cggaacgaat cactagagta
    42661 cttttacgac aattttcaaa caatgattat ggatgcatca attgatctgg caggtgctgg
    42721 atatgccaat taaatcaaat tataagaagc ctaaaattgt agctggtgat ctaaatacgc
    42781 cagttacttt ttttgagttt caacctaata agggtccaga acctggtgat gaagaaaaaa
    42841 aggaactgta tttttgtaca gcactagttt acaacccatc aatgaaagat cgtgacattc
    42901 ttggcacaaa agaaggcgtt acgataaaaa tacgggatcc ccatcaatct tatattccta
    42961 ctaacaaaca taaagtaagg atattagatt atcgatatgg agataaagtt tgggaaataa
    43021 tcgatgtctc accggatttg gaagaaaatt caattgtaaa gatagtatta gaattaatta
    43081 cttaaatttg gtgataaacg atgagtaacg taaagtttga aggcgttcaa gaaacaatcc
    43141 aagctttaga aaaaaagttt ggagaaaaga aaactaagac attaacaaaa aaagcaatca
    43201 atgttggcgc tgaaaaagta gaaaaacagt tgcaaacgga catgaccgtt tttaaagaca
    43261 aaggttatac gattgatgag gttgtacgta agaacgctac gtatagaaat tacaaggcag
    43321 aagctgaaat cggttggaat ggtcctcatc agcgttatag aatcattcac ttaaatgaat
    43381 ggggatatac acgaaaaggt aagcaaatta ggccaagagg ttttggcgta atcactaaat
    43441 cgcttaaaaa ttccgagcca ctatattttg atgctgttgg ggaggaggtt aaaaagagat
    43501 tatgaaagat atgctcaata tcatttatga ggctctttgt ttgaatgagt atattcgttc
    43561 aatgacatat aacgagaata ccgagcaata tcgtattaaa ttttatgagc aacctgaaac
    43621 cgccgataaa acagggccaa tgatcacact tcgtcctgta gatacaccta acgaagcata
    43681 tcacggaagt aacaaagagc tttctgtaga gcatatggta cagattgatg ttgaatcgaa
    43741 atatagagca acatgcaagc agattcaata tgagataaaa aaagagatga aaaaactagg
    43801 ctttggccaa atttctggtc aagggttaga tgaatacttt tctgaaacaa aacgctttgt
    43861 agacgcgcgt cgttacgaag gaaatacaaa aatttacgat accgaatatt agaaattaag
    43921 acacgaaaat aacgtgtctt tttttattac ccaaaattag gaggaaaatt ttatgacttt
    43981 agtaggattc aaaaaaatga caatcgggat ctttgacagc acagggaaga ttccagcagc
    44041 aaatttatat gtaattgaag gtgaacaaga caaaggggcg actgtatctg ctgaaatcag
    44101 tgggctatca aaagaaccat caaaagttta cggatcgaac atcgcttact atgtctctca
    44161 aaaaggaaca ggcgatgttt cagctacttt tggattgtta gatttaccaa cagaagtaaa
    44221 tgacaaaatt cttggttaca aaaccgatac aaacaaaatc agcttcttgg gagaagatac
    44281 tgagccacca tattgtgcaa ttttgatgga atcggaggat ttgaacggcg atacagcaat
    44341 gcttgcgatg ttcaaaggaa aattcagtcg tgaatctatt aatcttaaca ctacgacaaa
    44401 tgaagctttc gaaccagaag cagaagaata tgtattttca gcgattgcca atgatgttga
    44461 aggtgatgct aaaggccaaa cagtcgcaaa atatgttgga gacgaagaag cttctattac
    44521 tgctttgaaa gagatggtat ttcctgcagc gggggagtaa ctagctccgt tgttggagca
    44581 atcactccca ctattaatgg ggtaacagtt gaattaagtt aggaggataa ctatgccaga
    44641 tacatttaaa atctataaaa aaaatggtac tgaatttaca aaagtcgcag agggtgaaag
    44701 tccactttct attactggaa ttgcagcaaa tactcaagtt gctaaaggag actaccaagc
    44761 tacgcgtgtg gttggaggtc aagaatcagt aaaagtagat attcctgcat ttaccactct
    44821 tccaatttca gttactggtg tgacgttgga taaaacgaca gctgaagtcg aacaaggcgg
    44881 aaccttaaaa ttaactccta ctgtaacacc agcaaatgca acagacaaaa caggaagctg
    44941 gagcagttcg aatacagctg tagctactgt tactggaggc aacgttacag taaaaacaga
    45001 cgctgaagtt ggcggaacta cagaagtttc atttactact acggatggcg gtaaagtagc
    45061 aaaatgtact attacagtta ttgaaaaagc agcgggctag tcttcggact agctttttta
    45121 tttggaaaat aatggaggaa tctaaatggc aaaagtaaga attgaattga aagaagaatc
    45181 aggaaagaaa gtctatgaaa acatagacac aacaggtaaa gattatcgaa aagctttaga
    45241 aacaatcaaa aagctaaatg aagatggagt aatgatttgg aatcgtttag acatttattt
    45301 ggattttgct gtaggtatct tcagggatag caaattgaca gctgaacaaa ttcttgatgg
    45361 cttaccatct gagaaggtaa tgagcacatt ggacgacatc ctaggagaag taatggggat
    45421 tgaaagtaat ccagacccag acgcaaaaaa gtaacacccg aagaagcaga agaaatgtac
    45481 ttaaacctat gcagagagtt agtgaaagcg ggatggtcac tatctgacat agaagataat
    45541 tcctttgaaa tgatgatgaa aattgcgtgc acttcgccta agaaagagaa acaaaaagaa
    45601 gttgatctta aagacttctt gaaatccatg tagagaggag gtaaacatat ggcaaatgga
    45661 aaacctttag gcaatatgat tataaaacta gacttagaca gttcagcttt ttcaaaaggt
    45721 ctagccggag ctaaaaacgc tgttaaccat caaatgaaag caatgaaatc acagatgcaa
    45781 gtaatgaact cttcagggaa ttctctcggt gctttacaag caaaatataa tggtttaggc
    45841 ggagttctac aagcaaatga aaagcaagta gaattgctca ctaaggctta taaggatagt
    45901 tttgactcta atggaaacgc tactgcttcg actgctaaat acgctaatca attgaatcaa
    45961 gcaacagcta gatcagcaag ttacgaatct cagatgaaaa caaccgttgg acagattgct
    46021 cggatgaaag tggaaacgga agggattacg gggaaactaa aagctaattc tgatgtattg
    46081 attaattctg ggaaaaaaat tgaagccttt ggtaaaggag tttctgggat aggatctgcc
    46141 ctaactgttg gagtaacagc tccaatagtg gcaggtgcaa ctgctgttac tagtgcagct
    46201 atttcttggg aaagtgcttt tgcaggagta aaaaagacaa atgatgaagt tgtagattcg
    46261 aatgggaacg tagtttactc ctacagtgat ttagaaaaag gccttcgtga tttagcgact
    46321 aaattgccag caagccacca agaaatagcg aacgttgctg aagcagcagg tcaattaggg
    46381 attaagacag aaaatgtagt tagtttcacc aagacaatga ttgatatggg cgaatctact
    46441 aatatgtcag cagaaacagc tgcaacttct ttagcacgat ttgctaatat aacccaaatg
    46501 tctcaaaaag actttgataa attaggatca gtcattgttg atctaggtaa caattttgct
    46561 acaaccgaat cggaaatcac tgaaatggga ctgcggttag cgggtgctgg taaacagatt
    46621 ggaatgagcc aaggcgaaat tctaggattt gctactgctc tatcctctgt tggtgtcgaa
    46681 gctgaagcag gtgggtctgc cttttcgaag gtcatgatcc agatgcaact agctgtagaa
    46741 aaaggatttg gcgcttttga tcagctgaaa cagatggcag aagaacaagg tgttccgtgg
    46801 gttaatttag taaatgctgt gagagacggt ggaaaatcgt taaaagcagt atcagagcaa
    46861 atgggattca cttcatctga tttaaagaaa atgtataaag aagcggacaa ttcgaaaagt
    46921 tcgcttgaaa attttgcgaa tgtggcaggt gttacaagtg aacaattcca gaagttgttc
    46981 aagagtgatc cttcagaagc aatcatcaag ttcattcaag gactgaaaga ttcagaaaaa
    47041 catgggactt cagcaatcaa agttctggat gacatggata taaaagaagt tcgactacgc
    47101 gacagtttat tgagagctgc aaatgctagt gacatattca aaggagcaat tgaaaaagga
    47161 aacggcgcgt ggaaacaaaa tacagcttta acaaatgaag ctaataaaag atacgaaaca
    47221 actgaatcaa agctaaagat gttgaaaaac gaagtgattg atacagcaat cgatcttgga
    47281 ggtccatttg tagacgcgtt gcgtgatggt ctaaatgtat caaaaccgtt gataaaaaac
    47341 ttagggaata tggcgaaagc tttctcagcc atgagtgatg atcaacaaag aaacattatc
    47401 aaatgggttg gattagcagc agcagctggt ccagcgctga agcttttagg aactggtact
    47461 tcagtaatag gtaaagttac ttcaactact ggaagattga cgaaaagcct tgttgattta
    47521 gcagcgaaag cagcagaaaa gaaggctctg gatgcctttg catcgtcggt aacaacagct
    47581 ggtgctgcag ctgcaaagac agcaggagct ggaggtattg gcgggttagt ccctatgatg
    47641 ggaaaaacag ctagtgctgc aagtactgct gcgggcgcta gcggaatagg cgcaatgaca
    47701 acatcactag gtttgttagg tcctgctcta cttggtattg ttggcgttgg tggcgcacta
    47761 gcagttggct atggagcatg gaaattattt ggtgaagaag cttggaattc ctcgcaaaga
    47821 gtaaagcaat ggggaacaga tgtcggtgag gaaatagata atactcttga cggagtacag
    47881 aagaatattg aaggtgcaaa tggtcaattc ggtcttctca aagagggttt tactgttgat
    47941 gatgcgaata atatggcggc taatttcgca accgctggaa catctttgga aacatcttta
    48001 acaggtagaa taaaggcatt agacgatgca ctaaaagggc tacctcaatc agttcaggat
    48061 gccatgaaag aagtagctga atcagaagaa gaaaccttag ggcaatctct ttctaaaata
    48121 aaagacaaca atgaagaaat aaaaaaaatc agagaaagag ccgcaaatca gaacagagac
    48181 ttgactgttt cagaactaca acagatacag gatctgatga aaggaacttc tcaagcgtat
    48241 gtggatacgc tcgatgtttc tgctaaagac agaaaaaaga tactatcagc catgaatggt
    48301 gatgttgcac aggctacaga agaagaagcg aagacgtggt tacaatcttt agcgaaacaa
    48361 aaagctgcaa ctaccgaaaa ttatagacaa atgcttgaag aaaaaaaggc ggctttaaag
    48421 gaaaagggag taagcaaaga attcattgat gcattagaag atgaaatgaa tgattacatc
    48481 aaaactacaa atcaaggatt cgatagccaa atagctgcta taacaaataa atatccacaa
    48541 ttgattaacg aagtgagttt atcaaatggt caattattta gcaaaacggc agctgctatg
    48601 gacaatactg gtcagtatac aaaagttttt ctcgaaagta atgaaaagat tttagcccaa
    48661 gcgaagaaaa catcggatga aatagcagat aatgccgaaa aaaatgcaga acaacttggt
    48721 atggtagctg atgaggctac gcatgctgga tcaacttgga atggattagt tttagacgaa
    48781 aaaacaggaa aagtaaaaac taacgtacaa gaagtagtga atgaagcaac gaaggatttc
    48841 actaaatgga atgaacttaa acctgttgtt catgatgcga aactaacaac gaacgcaaaa
    48901 gatgtcattt ccatagcggc agtacaaaat ggttattggg atgatatgga atggaaagaa
    48961 aaagaagcat tattagaaga tgagtgttct aaaaatgtat acaaggcttt agaaaatgct
    49021 ggtaaatgga atgagttaga tattccttct aaagaagcaa tcctaacttc aaatactcct
    49081 gaagttatgg gtgaaactct catgaatttg ggactatggg atacttacaa acttcaagtg
    49141 aaagatttag atttgaatgc gtataaagta tacgatgcta tcgatggatc agaagaaaaa
    49201 ttaaatagtt gggctcaatt agataacccc acaaaagaat tgttattaga taactcagat
    49261 ttttctacaa aactttttaa ttccgaagaa atgtttaaac gttggaatca gttatcatct
    49321 aatgcaaagt ttctttcagc taataatact aaatttacag aggttctttt atcatctgac
    49381 agaatgtgga atcaatgggt aaaccttcct gataatgaga aaagattact tgcagataac
    49441 gaagatttag ctaccactgt tttatcttca gaagaaatat acggaagatg gactcaatta
    49501 ccagataatc aaaaatatat gttagctgac aatactgact tagcaaacaa aatatttgct
    49561 tcagaagaat catggaatgc atggaaagca attccagaca tagttaaaaa cttgcttggt
    49621 aataatattg atttactggc taaagtgaaa gatgggactg taagtgtaga agaatataac
    49681 aaagttatgc cacttcttaa aactctatat ggagataacg ttaatttaaa agaggcaatc
    49741 agtggggcta aaagctctat agagaattac aacaaaaata tttttcctaa tgataaaact
    49801 gcagtaggac acgatgaggc ttcacaagct gctaaagatg catttgaagc gttcaacatc
    49861 ttccaaacaa aaataccaga taaaattact aaaactgtat cagctgactt tataggtcct
    49921 atgcctaatg ctaaaggaac gaacttccat cctggcggag cagctatggt aaacgatcaa
    49981 aaaggaccta cttatgaaga gcttataacc ttgccaagcg gagaagcttt tatacctaaa
    50041 ggacgtaatg tcgttttaga cttaccaaga ggttcgaaag tactgaacgc cactaaaact
    50101 aaacgtctag tacctaaata tgctgatggt ataggaaaca taacgacggt ttcatctacg
    50161 ctaaacttag atgctttaat tttagctatt aacgagctaa cgacggtatt gagaactcaa
    50221 ctgatggcag aaacgactaa tgctaaagat actaaactat cagatgaaac aaaagttgcc
    50281 gatccgatta ttccgggcag tctttcagaa aaatcagatc agtatctagg aattggagct
    50341 caatggttaa ccaatctaat gaatggttgg aactcagcgg ttcctcaata tatgaatagt
    50401 gaaatggttt ttattacaaa ctatctcaat gcgctaaaac ttcaaaacaa tcctaattat
    50461 acgcaaggag tagcatggaa taagaactta ctgaatggat ggaatagttt aactgggaca
    50521 tttatttcta cgatcaattc attttgtaat caagcaatgg tcacgcttag aaactacaac
    50581 actcctatgt acaacaatgg ccgaacgtgg cagcaaaata atcttaatgg ttggaattca
    50641 ttgtatggat cattcatagc ccgtgtaaat cagcttggta acgattcgat taacaacctt
    50701 cgttctaaaa gtggtggttt ttacaatgca gggacattct tgttgcaatc tttaataaac
    50761 ggaatgaatt cgttaggtaa ttctctttct acaacaatga acaatgtcgc taatacaatg
    50821 gttggtggta tgggcaaagg ggttaatggc gtcatatcgg gagttaacta tgtgcttaag
    50881 gaagtcgagt caagtaagaa tatcggaaac tggccaattc cacaatacgc taagggaact
    50941 gatggacatc ctggtggact tgctatgatt aatgatcaaa aaggcctagt tcacgaagaa
    51001 tatgtacaga tgccagatgg acgtggtttt attgccaaag gtagagacct tcttgttaat
    51061 ttgcctaaag gtgctcaggt cttaaatgct tctttaacaa agaaattaaa ggaacgttta
    51121 aatattccac actacgaaaa aggtattggc aatttagata ttgttgattt gttagatgat
    51181 gaaaaaagaa tgttagaatt cttgactagc aaagttgact tttcaggcat taatgaacca
    51241 tggcttgata tgactaaatc aggaacgtca ttaatgtcta aagctgcaaa tacaatgctt
    51301 caatcaaaat taagtgaatt cttcactcat ggcaattttg atggtgcagt aaatgccaat
    51361 ggtgtttatc aatatttagt tgatgttgca cagaaagtaa tgggtaagtt ccccggactt
    51421 acagtaactt ctggatatcg agcaggagat gcttactatc acggaaaacg gcaagctata
    51481 gacttggctt atccaggtat atctggagat ccgagatata cagcagcagc taattacgct
    51541 tttgaaaaat tcccttcaaa aattgcgtat gtcattacga atggacgtgt acgtgaccgc
    51601 atgggattat ctggaacagg gtcaagcgga caatggacga attggccaga tggcgatcac
    51661 ttcgatcata ttcatttaaa cggttcgatg gggtctggcg atatattcaa tggaggagtt
    51721 ggaggaagtg gtgttgcacg ctggcgttct tatgtgtcta aagcattgaa aatgaatgga
    51781 ttaccagcta cagcagctta tataaatgcc tggatgtccc aaatccaaac ggaatctggt
    51841 ggtaatgaga aagcaatcgg cggcaatgat gggctcgctg aaggcaatgc aacaggatta
    51901 cttcaaacga aacccggaac ttttgcagct aacgcttttc ctggtcacgg aaatattatg
    51961 aacggatttg ataacatgtt agcagcaatc aattatgcta agaaacgtta cggagttgca
    52021 ggtatgttgc aagttatcgg gaaaggacat ggatatgcca atggtggatt gattaccaaa
    52081 gacggactat atcgagcggg agaaggaaat aaaccagaaa tggttattcc attgacaaga
    52141 aaaacaagag ctattgaatt gatgggtcaa gcattagctt ttctttctgg cgatgacaag
    52201 aaacgctcca acactacaaa tacagctgat aattcagcag agcttgtaac tttgattaaa
    52261 cagcaacaaa aacagcataa tgagttaatg atgattctta gagcgattct tggaaaagat
    52321 ttaagtctca agtcttctga cattgggcaa gctgctaatc attatatggg ctctgatttg
    52381 aataaacttc gatatacgaa tggaggtgtt tgataattgt tttacaaatt acagttcaat
    52441 caaaatggga agttgtttga tccgcaaaga aaagaaaaaa ttgtatgtaa agaaatcaaa
    52501 cgtcaagcac cagtgtatga agtgaattac gaagattttg aagggacgaa cggtagcaga
    52561 gaagctaatg ctagttttcg tccttttgaa ttagtgctga cttttgatat cttttacaaa
    52621 aataagcatg ataaagaact gctattaaca gagttctatg agctaatttt tgtcggttat
    52681 caatattaca tttcctatga tttaagccca ggaaaaagat ttaaagtaaa tccaacaaat
    52741 ttcgaaatta ctgaggaaga gaacgattat tctacgatag aaataacctt tagtattcca
    52801 tcagggagtg ctgaatccag cgcaaccact ttaacggatt tcaatctaga ggaagaatgg
    52861 caattctcgc aaggtctagt ggcggaagat tacaagtata cgcatcagac tagtcacttc
    52921 attatttaca atgctggaag ctttgaaatt gatccacgtg aacattatct acggatcgca
    52981 ttagaaggag aatcagaagg aaatgtgacg attttcaata aaacaactgg cgatcgattt
    53041 atttactatc catcactttc tacgaatctc ggacagacat tagtcttgga tggcgtgatt
    53101 ccaaaattga acggtgtaag ttgtggaatc aatacgaatc acggtctgat caatttggtt
    53161 gagggagtca atgaaatcga gatccaaaat attactcgcg tgaaatcttc atgggatttc
    53221 cgtttcttat ataagtaggt gattgagtga ctgagttaat tattcgaaat tatgaacaaa
    53281 ccaaagaaga aatccttgtc ggttatgaca agggttcttt ttatgaaaac tggcaacaaa
    53341 atgaaacgtg ggagattggc tttaccgtta ccagcaattc gttgaatcaa gaagtatttg
    53401 atttagtcga atacgagtct tctgttttct acaacggaca ggaatttgtg atcaaagaaa
    53461 tgactcgcaa agcacttgga caattgctga cgaaacaagt agttgcgaca catatctatt
    53521 acaccgttca agatggttat cagtacaaca cagtgacagg tgcgaaatct attagccagt
    53581 tactcacgca tgtatttagt gcagggagtc gcggctttac atgggaagtc attgatccaa
    53641 acaaaaaatt ccttaccgtt gaacaagaaa atttcggtaa cgcgaattac ttgaagctga
    53701 tcaatgagat tctatctgac tataatgcag tcgtgattcc gaataataaa catctaactt
    53761 tctatcctgc cagtgagtac ggccagcaga cggaagaaca gattcgctat aaatacaata
    53821 cagatgaagt gtcgtttgat attgatacgt attccttgaa gacgcagatc aaaggctatg
    53881 gaaaattgaa ggatggtgca aatactgaga atcctaaaga tagtgactat atatttactc
    53941 ccatcactta tacaagccct gaatcacaga agtggggaat caggatacaa gatcctgtta
    54001 aagacgaacg gtataccgta tcagggaaca tgctcgagcg gttaaagaca gacttgcaag
    54061 actatccaaa tatttcggga tccgtaacct tgaaatggaa aatcagtccc aacaaaggcg
    54121 atcacgtccc attcatttat gaacctttga atattaatac gtacattcaa gtggtaggaa
    54181 tcaagacgta tccagcgata ccaaataagc caccagaaat cacattgagc aacacaaaga
    54241 aaacaatgac gtcgatactc gctgaaatgg cgaagaaagg agtgatttga tggggttatt
    54301 aaaattaatc agtaaccgta tctctacgga atggaaagag aaatttaata aaaacattga
    54361 ctaccttaat gatcttgaaa agaaactgtc tgatcaagac aaatcaatga acagtcgtat
    54421 tgataatctc gtgcttcatt caggtggcga atcgccgaat gaagtggttg atgctcgtgt
    54481 aaataataag ggagaaacct ttcctacttt acacggcaga ttggtagaac acgaaaccct
    54541 gacagatgaa caaattagcg aactgaatac aaacatggat agtcaaaaag agcaagttca
    54601 acaattgaac aagtctgttc aacaaattat tggtggatac aatgagccaa ttaacatcta
    54661 tgtttctaaa gatggtagtg atattttggg tgatggatca gaagaaaagc ccttcttaac
    54721 cattcaaaca gccgtaaata atgttcctct aattacaacg tctcaaatta ctatttgggt
    54781 aggtagtggg gcttacttag aagatattat gattagaaat ttaaatttta catcattttt
    54841 aattcgacct atcgataatt ttgacactat tgatccatta aaatcagatt tacctgttaa
    54901 aatacgatcc atttgtttca ctgcttgcaa aggttattgt caagttgctg gaatgcaaat
    54961 tgtggataca gcaaacggag cagactatgg aatcagaaat gagcaaagtg gctatatggc
    55021 aatcaataga tgtaaattcg cagagaacac aaaaacactt gcaagatata atgcagttta
    55081 tgtcggcgga acatcaaaga ttaatatgta cggagacacg acgttgatta accagaaagt
    55141 ggcaatttac gctgtattga tgggtgaaat ttttgtcagt gcgtttggtt caggaaacga
    55201 cgtaggaatt ttgtgcgaga atggcactgt acgaggaact gtatcaactt cttttgccac
    55261 aaccccaaca aaaactactg ggtacggtct catcatcacg aaaggcacgg tgttgcaata
    55321 atggtataca aaacaaatga atcgatcatc atgattcaag cagaagccac tagtccaaac
    55381 aggacgaatg ttgttttttg gtcgcatgat cgaggaacag ctaagcttcg aatgaaatta
    55441 gttcggaaaa acggcattcc tcaaagctta cccgaaggca caactgttcc gattcgcttg
    55501 atgctcaaat ctgcaacggc agaaggtggt tatggaaaac atgactatct cgccaccatt
    55561 gaagatcgtg taaatggtat tgtgtccatt gtgctggaag ataatatttt aggctatgtt
    55621 ggaattgtcg aaggtagtgt ttatattgat ttcccaaacg accgctcgtt agatacagct
    55681 ggccgtttta cttttgacat caaacgcagt ccaatcgatg atagtacacc agaattggaa
    55741 gattattatt tcaatggttt cagtcagact attgataaaa tcgaaaaaat tctagctgat
    55801 ggaaagcaag agattgaaca gaaaattacg gaatctgaaa cgcagattga tgggaaactg
    55861 aaagaaacaa gcaacaaaat cacgaaagcc aatcaagatg tcgcaactat caatacgaat
    55921 attgataaag caaatgatcg tattgatcaa acgaaccagc aaataagtga tctcggaaag
    55981 ctgaaaaaga tgtactccaa cagcatcgat ttcgggaact atgattatag tggaaatccg
    56041 aatttgatgg ctaatataaa cgctgatagc ttctcgcaag gtagtggtgc cttatctgtt
    56101 gtagatgatg gtgacgaggt agtgattacg cttgatccaa accataaatt agaaatattc
    56161 aaagcgaaaa gtcaaccagc tctggtagtg gggaaaacat ataccatgag tgtagaaatt
    56221 atgttagaag atgatttcac tggagaccct agtaatatat ctctaagata tattaaaatg
    56281 cccagctggt taacagtact atatacgcct tatacattaa ctaatactaa gggtgtatgg
    56341 caaaaactaa ctgttaccgc taaaatgact actgcgattg ataacgctga aagctggttt
    56401 ataatgctac aaaataaaga cgctaataac agcctatccg gcaaactacg tttgagacac
    56461 gctaaactcg aagaaggctc aacagctaca ccattccaac ctaacttatt agcagaacct
    56521 tacaacatgt gtcgcgaata tcctaacgaa aatattgccg atcctaaagt taagttccca
    56581 atcgaatctg gcgaccacca aatatatcaa ggctacacag aagaagagct tatgataggt
    56641 caaacgtata ctatcacgct taaaggaaca aaacccgcaa gtcaaacctt tgtagcgtat
    56701 aatcattgga ctgctcgttt aggagaacta aagccggttg atgggttgac agacgtatgg
    56761 tctctaacat tcacaccaac gaatgttgtg gcgatgccta aacttttccg tgtttatcag
    56821 tatccacgat caacagtagg cgcatgccaa attgactggc tcaagattga aaaaggcaac
    56881 acccgaacgc ccaatattag tcagtttaaa tacttcggtg aaggattgaa agacagcaac
    56941 aatccgaatg attacagttg ggatgtcaca cctgaatata ctgaaaaagg cttgaataat
    57001 gctgttaatg tgtatgatcc tcagagagtt gaaggtttga aaaactttgc cgatggtatt
    57061 caaatagcag gagataaagt gatcagtgaa aatgattgca ctgtttatac attaactaaa
    57121 gacaacagtc aatcgtttat cgatgggtat gcaacattta ttaaacacgg aaaagaagtt
    57181 gttgtaaatg gtacagtaaa gttcaaaaaa gcttatgctt ttggtgtacc acttgatgat
    57241 gaagtcccag atgaatttat tgcaagaatt gttcatggca tgcttacagg tcagtcagga
    57301 acaaatagtg tatcaaaagc gatgtacgtg caaaaagact taggaaaaat tatgacgaat
    57361 agcgaatttg ccgcaaatga atggtttaca ttccacggta attattgggt aggagtgaaa
    57421 taaatgaaaa acatttggaa atacggacgt actggtggag agtacgcagg aaaagtattg
    57481 gacgacatgc ttgtatccgt tccttacacg gatcagccac cgctcgaagg attacgtgct
    57541 gatggcgaac cgctaacgat tgctgatcag atgtttgatc ctaaactgaa ccaatggatt
    57601 gttttagcga acacactgga tcacaacgat ttaaacaatc tcaaagcgat gtacgaggca
    57661 ctggaacacg agaacggcga tttaaaacag ctcaacgcca aactcatgct aagcgatgta
    57721 gcaattaaac aggaaaatac tgcattgaaa gaaaaagcgg atagtttagc acaaatcaat
    57781 tcaaaaatga tgcttgcttc gttacaaaat agcaaagaca tttcagaaat taaagagcaa
    57841 ctaaatccaa ctttaaaggg aggtgagtag tatgtttagt tttagcgatg tgaaaatgat
    57901 gtatgattgg ggctgtttta ctgacgatca agttcgacta ttcgttccac tatgcattac
    57961 agacgaagaa gcagataaaa tcattagcaa agaagagagc gcatcttaag tgatgcgttt
    58021 ttttgttgga aagttggtgg aacatgaaag aagaagcgct ccaagacgtt gtggagagat
    58081 tagtaagaat tgaaacaaaa ttagacaact acgaatcact tagagaaaag gctgatagtg
    58141 caaaagattt agcagataaa gcctattcag tagcactaaa caatgcagaa gacatcaagg
    58201 aaatgaagaa caataataaa tgggcttggg gctatatgat tggcttaggc attacgatca
    58261 ttggctattt cttgactaaa ttgtaaagga ggtgagaaga aatgatttta ccagataagt
    58321 attatcaagt cattaaatgg acagttttaa cagtattgcc agctgcatct gctttagtag
    58381 ccatgttagg gaaagcgtat ggatggaatg gaacagatat gatagtgttg actatcaatg
    58441 cagtagcaac atttttaggc gttatcactg gtgtgtcggc ttataatttg aaaaaatagg
    58501 aggaaacaaa tgaaaaagaa aattactatt actgcgatga gcctattaac ggctcttttt
    58561 ttattgccaa ttaatgggtt tgcctatact atcaacaatg aatttaattt gggcgcaaat
    58621 gaaggtagct cacaaatagc aaataatcag tacattttac tgcatgaaac ggctaatgaa
    58681 acagcaacag gacgcaatga agcgcagtat atgcaacgtt catggactag cgcttatact
    58741 gcttatattg tgggagacgg cggaattgtt tatcaagtcg gtcaacctgg ttatgtacag
    58801 tacggtgctg gttcgtatgc taatgccaac agtcctgtgc agattgagtt acaacacaca
    58861 catgataaag caacgtttga gaaaaactac aaggcatacg ttgaattggc tagagattca
    58921 gcaatgaaat atggtattcc attaacatta gacacgcctt ataaccaacc aggaatcaaa
    58981 tcacatttat gggtaacaca aaatatctgg ggagatcata ctgatccata cggttatctt
    59041 tctgaaatgg gcgtaagtaa agaaaaatta gc
//
LOCUS       DACDIP010000011        53974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDIP010000011 DACDIP010000000
VERSION     DACDIP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.011"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..104
                     /locus_tag="IV257_000828"
     CDS             <1..104
                     /locus_tag="IV257_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8813976.1"
                     /translation="LESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            136..858
                     /locus_tag="IV257_000829"
     CDS             136..858
                     /locus_tag="IV257_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8813977.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            916..2955
                     /locus_tag="IV257_000830"
     CDS             916..2955
                     /locus_tag="IV257_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8813978.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3064..4215)
                     /locus_tag="IV257_000831"
     CDS             complement(3064..4215)
                     /locus_tag="IV257_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8813979.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4369..4899)
                     /locus_tag="IV257_000832"
     CDS             complement(4369..4899)
                     /locus_tag="IV257_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813980.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4998..5348)
                     /locus_tag="IV257_000833"
     CDS             complement(4998..5348)
                     /locus_tag="IV257_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8813981.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5505..7034)
                     /gene="ahpF"
                     /locus_tag="IV257_000834"
     CDS             complement(5505..7034)
                     /gene="ahpF"
                     /locus_tag="IV257_000834"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8813982.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7044..7607)
                     /gene="ahpC"
                     /locus_tag="IV257_000835"
     CDS             complement(7044..7607)
                     /gene="ahpC"
                     /locus_tag="IV257_000835"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8813983.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7751..8413)
                     /locus_tag="IV257_000836"
     CDS             complement(7751..8413)
                     /locus_tag="IV257_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8813984.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8597..10243
                     /locus_tag="IV257_000837"
     CDS             8597..10243
                     /locus_tag="IV257_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8813985.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10234..10362
                     /locus_tag="IV257_000838"
     CDS             10234..10362
                     /locus_tag="IV257_000838"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8813986.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            10329..11393
                     /gene="uxuA"
                     /locus_tag="IV257_000839"
     CDS             10329..11393
                     /gene="uxuA"
                     /locus_tag="IV257_000839"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8813987.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(11501..13054)
                     /locus_tag="IV257_000840"
     CDS             complement(11501..13054)
                     /locus_tag="IV257_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8813988.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVRKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13202..14490
                     /locus_tag="IV257_000841"
                     /pseudo
     CDS             13202..14490
                     /locus_tag="IV257_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14616..15695)
                     /locus_tag="IV257_000842"
     CDS             complement(14616..15695)
                     /locus_tag="IV257_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8813989.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15798..16238)
                     /locus_tag="IV257_000843"
     CDS             complement(15798..16238)
                     /locus_tag="IV257_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8813990.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16295..16705)
                     /locus_tag="IV257_000844"
     CDS             complement(16295..16705)
                     /locus_tag="IV257_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8813991.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16885..17319)
                     /locus_tag="IV257_000845"
     CDS             complement(16885..17319)
                     /locus_tag="IV257_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8813992.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17419..18267)
                     /locus_tag="IV257_000846"
     CDS             complement(17419..18267)
                     /locus_tag="IV257_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8813993.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18656..20422
                     /locus_tag="IV257_000847"
     CDS             18656..20422
                     /locus_tag="IV257_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8813994.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20524..21783
                     /locus_tag="IV257_000848"
     CDS             20524..21783
                     /locus_tag="IV257_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8813995.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21930..23231
                     /locus_tag="IV257_000849"
     CDS             21930..23231
                     /locus_tag="IV257_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8813996.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23234..24085
                     /locus_tag="IV257_000850"
     CDS             23234..24085
                     /locus_tag="IV257_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8813997.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            24098..24913
                     /locus_tag="IV257_000851"
     CDS             24098..24913
                     /locus_tag="IV257_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8813998.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            25064..26230
                     /locus_tag="IV257_000852"
     CDS             25064..26230
                     /locus_tag="IV257_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8813999.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26460..27107
                     /locus_tag="IV257_000853"
     CDS             26460..27107
                     /locus_tag="IV257_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8814000.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            27191..28135
                     /locus_tag="IV257_000854"
     CDS             27191..28135
                     /locus_tag="IV257_000854"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8814001.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28270..29721
                     /locus_tag="IV257_000855"
     CDS             28270..29721
                     /locus_tag="IV257_000855"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8814002.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29742..30578
                     /locus_tag="IV257_000856"
     CDS             29742..30578
                     /locus_tag="IV257_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8814003.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30578..31237
                     /locus_tag="IV257_000857"
     CDS             30578..31237
                     /locus_tag="IV257_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8814004.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31363..32052
                     /locus_tag="IV257_000858"
     CDS             31363..32052
                     /locus_tag="IV257_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814005.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            32058..33014
                     /locus_tag="IV257_000859"
     CDS             32058..33014
                     /locus_tag="IV257_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8814006.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            33058..33570
                     /locus_tag="IV257_000860"
     CDS             33058..33570
                     /locus_tag="IV257_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8814007.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33826..34374)
                     /locus_tag="IV257_000861"
     CDS             complement(33826..34374)
                     /locus_tag="IV257_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8814008.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34463..35644)
                     /locus_tag="IV257_000862"
     CDS             complement(34463..35644)
                     /locus_tag="IV257_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8814009.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35880..37388)
                     /locus_tag="IV257_000863"
     CDS             complement(35880..37388)
                     /locus_tag="IV257_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8814010.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37547..37738
                     /locus_tag="IV257_000864"
     CDS             37547..37738
                     /locus_tag="IV257_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814011.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37941..38858
                     /locus_tag="IV257_000865"
     CDS             37941..38858
                     /locus_tag="IV257_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8814012.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38851..39924
                     /locus_tag="IV257_000866"
     CDS             38851..39924
                     /locus_tag="IV257_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814013.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            40072..40590
                     /locus_tag="IV257_000867"
     CDS             40072..40590
                     /locus_tag="IV257_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814014.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40571..40963
                     /locus_tag="IV257_000868"
     CDS             40571..40963
                     /locus_tag="IV257_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8814015.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            41039..41974
                     /locus_tag="IV257_000869"
     CDS             41039..41974
                     /locus_tag="IV257_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8814016.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            42107..42886
                     /locus_tag="IV257_000870"
     CDS             42107..42886
                     /locus_tag="IV257_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8814017.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42879..43196
                     /locus_tag="IV257_000871"
     CDS             42879..43196
                     /locus_tag="IV257_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8814018.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43440..45050
                     /locus_tag="IV257_000872"
     CDS             43440..45050
                     /locus_tag="IV257_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8814019.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45207..46055
                     /locus_tag="IV257_000873"
     CDS             45207..46055
                     /locus_tag="IV257_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8814020.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46286..46765
                     /locus_tag="IV257_000874"
     CDS             46286..46765
                     /locus_tag="IV257_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814021.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46798..49026
                     /locus_tag="IV257_000875"
     CDS             46798..49026
                     /locus_tag="IV257_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8814022.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            49155..50798
                     /locus_tag="IV257_000876"
     CDS             49155..50798
                     /locus_tag="IV257_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8814023.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(50871..51416)
                     /locus_tag="IV257_000877"
     CDS             complement(50871..51416)
                     /locus_tag="IV257_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8814024.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(51439..51924)
                     /locus_tag="IV257_000878"
     CDS             complement(51439..51924)
                     /locus_tag="IV257_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8814025.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(51914..52726)
                     /locus_tag="IV257_000879"
     CDS             complement(51914..52726)
                     /locus_tag="IV257_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8814026.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(52865..>53974)
                     /locus_tag="IV257_000880"
     CDS             complement(52865..>53974)
                     /locus_tag="IV257_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8814027.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        17973 a         9814 c        10429 g        15758 t
ORIGIN      
        1 ggctagagag tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac
       61 aacgacgttt tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac
      121 gggttattat ctattttgtt actgcttaca ttagtagttg gctatttttc gattgagttt
      181 atccatggat tttcgtctgc aaaacagagt tcaactgtga aaaaggtaga gccgaaaagt
      241 atccctacca cactaaatgt ggctttgatt ggttcagatg ctcgttcgaa agacgaaaac
      301 ggtcgctcgg attcacttct ggttgcacaa tacaaccaga aaacacaaca agcaaaactg
      361 atctctatca tgagagattc atatgtcgat attccaggtt acggaatgaa taagataaat
      421 gcagcttact cttatggggg agttgattta ttaaatcaaa cattaaagga aaatttcaaa
      481 tttgaagctc cctattatgc aagtatcaca tttcaagatt ttatcgattg cgttaatgaa
      541 ctgtttcctg gtggagtcaa gatcgatgca gaaaaatctc tagatttaga tggcgtatat
      601 ataaacaaag gagagcaagt gatggatggt aatactttac tccagtatgc tcgatttcgg
      661 gaagacaaag aaggagactt cgggaggatc agaaggcagc agcaagtgat caaagctatc
      721 tctcagcagc tgaaagacgt aacttcaata ttcaagctac ccaaagcggt tgggaaatta
      781 cttggtagta tccaaacgaa ccttccagaa agtgtgcttc tggattgtgg tatggatttc
      841 ctcaaagaca tatgttagaa taaacaggta taaatagtga aataaaggaa tgacaagcaa
      901 gagaaggagg aaaaaatgaa aagaagtgac aagcacggca aaaatcgaac aggcgcttat
      961 attgccggcg cagtgatctt aatagcagct gcgggtggcg gttattttta ctaccagcac
     1021 tatcaagaaa cccaagcagt agaagctgga gaaaagacag ttgagcaatt tgtccaagct
     1081 ttgaacaagg gagattataa caaagctgca gaaatgacat caaaaaaggc ggcaaataaa
     1141 agtgcattat ctgaaaaaga gatcttagat aaataccaga atatatacgg tgctgctgat
     1201 gtcaaaggac ttcagatatc gaatctaaaa gtagataaaa aagacgattc tacttacagt
     1261 ttttcatata aagcaaagat gaatacctca ttaggtgaat tgaaagatct ttcttataaa
     1321 ggaacattag acagaaatga tggtcaaacc acgatcaact ggcaacccaa cttggttttt
     1381 ccagaaatgg aaggaaatga caaagtaagc ctgaccacac aagaagcagc aagagggaat
     1441 attatagatc gaaatggtga accattagca acaacaggca aactaaaaca attaggagtc
     1501 gttccaagta aacttggaga tggaggcgaa aaaacagcca atatcaaagc gattgcttcc
     1561 tccttcgact taacagaaga tgctatcaat caggcgattt cacaaagctg ggtacaaccc
     1621 gattactttg tcccattgaa aatcattgat ggagcaacgc cagaacttcc agctggagct
     1681 accattcaag aagtagacgg cagatattat cctttgggtg aagcagctgc tcagctgatt
     1741 ggttacgtgg gagatatcac agcagaagat attgataaga atccagaatt aagcagtaat
     1801 ggaaaaatcg gtcgctcagg tttggagatg gcttttgata aggatcttcg tgggacaaca
     1861 ggtgggaaat taagcattac agatgcagac ggtgtagaga aaaaagttct gatcgaacat
     1921 gaagttcaaa atggcaaaga tatcaaattg acaatcgatg cgaaggcaca aaaaacagct
     1981 tttgacagtc taggaggaaa agctggatca actgttgcga caacgccaaa aaccggtgat
     2041 cttcttgcgc ttgctagctc tccaagctat gatccaaaca aaatgacaaa cgggatctca
     2101 caagaagatt acaaagctta tgaagaaaat cctgaacatc cattcatcag ccgatttgcg
     2161 acaggttatg ctcctggatc tacgtttaaa ataattacag cagcaatcgg tctcgacaac
     2221 ggcactatcg atccaaatga agtgttgacg atcaacgggc ttaaatggca aaaagacagt
     2281 tcttggggat cttttcaaac aacgcgtgtc agtagtgatg tatcacaagt agacttaaaa
     2341 actgctttga tctattccga taatatatat gcggcacaag aaacgttgaa aatgggtgag
     2401 aaaaaatttc gtacaggctt agataaattc atttttggtg aagaccttga tttgccaatt
     2461 agtatgaatc cagcacaaat ttctaatgaa gatagcttta actcagatat cttgctagct
     2521 gatactggat atggacaggg cgaacttcta attaatccta tccagcaagc agcaatgtat
     2581 tctgttttta ccaacaatgg cacacttgtc tatcctaaat tgattgcaga taaagagaca
     2641 aaagataaga agaatgtcat cagcgaaaca gcagtacaaa cgatcgtgcc agatctgaga
     2701 gaagttgtgc aagatgtaaa tggtacagca cattctcttt ctgctttagg gattccgttg
     2761 gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg atgtaaaggg gcaagagaac
     2821 agtttcttgt ttgctttcaa tcttgataac caaggttata tgatggtcag catgttggaa
     2881 aataaagaag atgatgattc agcaaccaaa cgagcatcgg aactattgca atacctcaac
     2941 caaaattatc aataaaaaat tgctttttaa gtacagtaaa aggactgtgg cgttatcgtc
     3001 acagtccttt tttcaaataa acggttttca cgatgtctct ttcacaactt ctttttgatt
     3061 tactcacact tcataaactt tttttgtaaa atatttgaga acaagtggag aaataagtgt
     3121 acttaataaa atcacgataa ctaaaggtga atagtactgt ggttcgatca attgactttg
     3181 ctgtccaatt tgtaaaatga tcaaagccat ttctccacgc gaaatcatac cagcaccgac
     3241 catcaatgaa ctatgttgag aaaaaccggc aatctgagca ccaaaataac ctccaagcaa
     3301 tttggtcaaa atcgctacaa gcgtcaatat agaaataaag agtagttgtt cactaaagtc
     3361 agaaaaatca acttctaatc ctacactgac aaaaaatact ggtataaata ctgcatatcc
     3421 taaagcctct acattgtggt atacttcttc tttaaccttt gtctgactga cagctatccc
     3481 agcaaaaaaa gcaccaatga cagagctaag cccaacaaga tccgctacgt aagacattcc
     3541 taaacaaata actagcgata cgatgataat agaagaattt gcgtacactt tttctgcaaa
     3601 tgaaagtagg aaaggcgcaa tccatttgac taataagaaa ataaaaacaa aataaattag
     3661 ctgttcaatc aaaacaagcg gtaaagaagc attgctagaa gcttcccccg ttagaaaaga
     3721 taggctgaaa cttagaacta aaacgactaa aatgtcatct accacagaag cacccaaaat
     3781 tgttgatcct tcttttgtat tcactacgtt tagttctttt aatacttcta ctgaaatact
     3841 gacagaagtt gccgcaagaa taatcccaaa gaaaatcgct tcatttcccg aaacttggaa
     3901 tgctttgcct gtcaaccagc caaaaacaac cgggaagatg atcccaagca aagcaacgaa
     3961 cataccaggt cggaaatatt ttttcaaaag agataaatca ctttccagac cagctaaaaa
     4021 cattaataag atcacaccaa tctccgaaaa gtcatggact agaatatcag gtttgaccca
     4081 attcaatcca gcttggccta gcaaaacacc aacaagtaat tgtccaatca ctgctgggat
     4141 acctaatctc cgcgagatat gtgagccaat cgttgttgaa atcaatatca ggcataaaat
     4201 accaataaat tccattctca tcattccttt gtataaaata tagcccgaac ttagcataac
     4261 atacattatt gaaaaaatag gaaaatatac tcacaaaaaa accaacaatc caaaagattt
     4321 ttttggattg ttggttttta tacggtctat tttaataact aaaaatgatt aacgtatagt
     4381 catttttctt attctgatga aattgataat ctcctggatg cccatgaaaa tcaaaataaa
     4441 gccgaatatt tggaatagaa gcatcaaact tttgaatgga ttgaacaata agacgaatcc
     4501 gccaatcaac aacagaatac tactataaac actccaactt ttagcttttg tattcatgct
     4561 gaagaacaac tggaacaagc tgttgatgat aacagctaat ccaagaagaa tcggcaggat
     4621 agaaacgatt gcaggtgcaa aagcatagac gatcagagct aagatcagga aaaaaagacc
     4681 actgaacaga ccgatactcc aagaacctgt tttcctttta aaacggtaag cctcaaaaag
     4741 attcaacaac ccaagcaaaa caagataggc ggtgatcaga taaccgataa aatgaaaaac
     4801 agcagaaggg tttagcacaa tcgcaatccc agaaataata taaacagcag ctcttaataa
     4861 ggcgtatttt tgaatatttt gtttaatcgt ctcaaacata taagaaaacc tcctttgact
     4921 tattttaacg tgaactagtc attttaccaa attaaatgtt acaaaatgag cgaaggagaa
     4981 gaatatctct ggaaaaatca acttctgagg atcttttcca tttttttgcc ttttgctagc
     5041 tcatcgatca ttttatctaa ataacgaatt tctttcatca agttatcctc gatgtcttct
     5101 actcgtacac cacaaacaac acctgtaatc aaggaacgtg aaggattggg gacaggggct
     5161 tccgcaaaaa attgttcgaa agtcacttgt ttttgtagtt gatattctag ttctgtttgg
     5221 gaataacccg tcagccaaca aatgatttca tctacttcct cttttatacg atcttttctc
     5281 tccgcttttg ctacatacat aggataaacg cttgcaaaac ttgtcgtaaa aatacgattt
     5341 tgcgccataa tcagtcacct ttcttttttt caaattattt actacttata gtatataaga
     5401 aaaagagaag aaaaagcgat tattcaaaaa aacaaaaagg attgtgacaa aattcttgtc
     5461 accatccttt tttccgctct ctgacttttt acaatcttca tagctcaagt gcgcatcaga
     5521 taatcgaatg ctcctaaagc tgcagtagct cctgaaccca ttgaaacaat gatttgtttg
     5581 tatggactat ctgtacaatc accagctgcg aaaacgccgg ggatattcgt tgcgccattt
     5641 ttctcggtca ctatttcatt tcttggattc atttcgatcg ttccatctag ccaatccgta
     5701 ttagggagta agccgactag aatgaatact cctgaaacag ctagtgtatg catctccatc
     5761 gtacggcgat cgatatagga gagagattct acctgattcg taccgctgat ttctttcgtc
     5821 gctgcatgtt tgataacagt gacattcgca agagagacta gtttatcctg taagatttga
     5881 tctgctttta attctggtaa gaattctaga acatatacat gttttgctaa cccagccaaa
     5941 tcgattgctg cttcaacacc agaattacca ccaccgataa cagcaatctc ttttccttta
     6001 aataaagggc cgtcacagtg tgggcaataa gcaattcctt tatttttaaa ctctttttcg
     6061 cctggtacat tgatagaacg ccagtgcgca cctgtcgcga tgatggcggt ttttgcttta
     6121 agtgatatac cgttagctaa gccaatatta ataaaatgtt gctctttatt taaggaaacg
     6181 gcctgttgat tcatcataat gtcgataggg taactacgca catgactttc tacttgctgc
     6241 atcaattttg gaccttctgt gtaagaggta ccaatcatat tttcaatccc tagtgtttca
     6301 acgacttgtc cgccgaaaga atcagcgaca agtcctgtac ggattccttt acgtgctgca
     6361 tagatagctg cactactagc tgctggtcca ccgccaatta ctaatacatc ataaggttct
     6421 ttttcaaaca actcttcttc aatcaaaggg ccagtgattt tttcaacaat ttgttcgatc
     6481 gtcatgcgtc cgctactaaa ttcttctcca tttaaaaaca cagttggcac tgccatgatt
     6541 tttttatcat cgatttcttt ttggaacatc ccaccttcaa tcatcgtatg agtaatattt
     6601 ggatttaata cagctaatat attcaatgcc tgtacaacgt cggggcaatt atgacaagtc
     6661 aagctaacat aactgtcaaa atgcaatgtt tgtgttattt ttttgatttt aagactcaaa
     6721 gattcactga ttttcggtgc ccgtccacta atttgtaaca tagccagtaa aaaagaggaa
     6781 aattcatgac cgagggggat accagcaaaa acgattccac tttctttgcc aactggattg
     6841 atagaaaaac taggtgtgcg catcgaatct cttttttccc aagagatttt aggagacatt
     6901 tctgctattt ccttcgtaaa ttcagctagt ttttgactat tttcactatt atccaaacta
     6961 acagaaaaaa caataggaga ttcaatcagt gctaaatatt gttcgagatt ttgtttagtt
     7021 tctttgtcta acatatccac cacctaaatc tttccaacaa gatcaaaact tggtttcaat
     7081 gtttcgccac tttctttcca ttttgccgga caaacttcac ctgggtgggt acgtacatat
     7141 tgagcagcgc gaatcttatc aatcagtgca ctagcgtcac gaccgattcc atctgcattg
     7201 atttccatag cttgaacgat tccgtctgga tcaataataa atgttcctcg ctgagctaat
     7261 ccttgctctt catccaaaac atcaaaaata cgagaaatct gatgagacgg atcaccgatc
     7321 atcacgtatt cgattttacc gatagcatct gatgtatcat gccatgcttt gtgagtaaag
     7381 tgggtatcgg tagagcaaga aaagacttca acacctaaag atttcaatgt actgtattgt
     7441 tcttgtaagt cttctaattc agtaggacaa acaaaagtaa aatcagctgg atagaagcaa
     7501 agaatactcc agttccccat aagatctttt tcactgactt ctaaaaattc tccttgatga
     7561 taagcatgtg cattaaattc ttcgattttt ttcccaatca atgacatagt aaaacctcca
     7621 ataataatta tttatttata attattgtaa tttagttgga gttgcaaagt caatggatat
     7681 tttgttattt aataaagatt actcaaaaac gaaggttaat tataaaaata gaagaggtcg
     7741 caagaaaatg ctactgctgg aaatactcgg gataacgttg agtaacagtt tcctgttctt
     7801 tcagcatcat gtgtaagtgc aatcccgttt gaaatcgagc ttcttctggt tttttatcaa
     7861 ctaaagcctt gagtaaatca taatgttgtt ccatgatcgt ttcccatgga agcccctgaa
     7921 tggtcactcg taaccagcgg aaacgttcta aatgggtatt atggtcatct aaccagttcc
     7981 aaacttcttt tcttcctgca atttcgaaac aagcttcatg aaataaatta tctgcacgga
     8041 aaaaagcgcg ttcgtcattt attttgactg ctttttcttg ttcttctaaa atcgtttcta
     8101 gcactattcg tgtttgtgtg tccaatttgg aacaacattc aatcatgatt tgctcttcca
     8161 actgttctcg gacaaagcga gcattttctg cagaaacaag attaattttg gaaacatacg
     8221 taccactttg aggtatcgta tacacgagtt cttgctgacg tagttgaatc aatgcttcgc
     8281 gaatgggagt ccggccgata gcaagatgtt cctccagtgc cttttcagat attttccttc
     8341 caggttctaa gtcagcatag ataatttgtt tacgaatcgc ttgataagct tgtgattgca
     8401 agctgctttt catctttatt cctccaaaat catttctatg aacgtagtat aacagaagag
     8461 gtctgaaaaa caatctggta tgtcacatta actagtaaat ttgtataaat agtaaaattt
     8521 tggttgacat agcgattttt attgtataaa attcacttga atacaaaaac taatatgtta
     8581 gtttaggagg ggaaaaatga ttgatttaaa caactggagg aactttatcc ctgaattatc
     8641 tcaaaaagaa gtagaaatac ctcattttga ccgtgcatca ttaagagaaa atggacaaaa
     8701 aaatcctgtc tggatccatt tcggtggcgg aaatctttat cgaggatttc atgcagaaat
     8761 cgctcaacgt ttagcggatc taggcgaatt gtccactggt gtcattgttt gtgaaacatt
     8821 cgatgaagaa ataattgaaa gggcttacca agattttgat gatttgattc ttgaagtggt
     8881 catgccaagc agtggagagt tcgtcaaacg tatattagct gcgacagctg acagtctgta
     8941 ttgtcatcca gattgttctg aacactttga taagatgatt cgacactttg aaaatcctag
     9001 tttacaatta gttacttttg ctattactga aaaaggttat cgattgaaag atacaaataa
     9061 taaatatata ggaccgattg cagtcgatat aaaaaatggt ccaaagaagc catcacatac
     9121 aatgagtatt cttgctgcgc tactttataa acgtttttta tctggagctt ggccgattgc
     9181 acttgtctct acagataatt tttcacaaaa cggtagtcaa ttacgtactt ctttgctaac
     9241 gatcgcaaaa gaatggcaaa aggaaggtta tgtacaaaat acatttatcc aatacttatc
     9301 gaatgaagaa attgttgcgt ttccatggac gatgattgat cgtatcaccc cgaatccttc
     9361 cttgtcagtt agcaaaatgt tggcaaaaga gggcttttca aatatgtcga ttttccaaac
     9421 cgcaaaagga aataatattg ctccatttgt caatacagag gacatccatt atttaatcat
     9481 ggaagatcat tttcccaatg gacgtccgaa attcgaaaaa gctggggtat tcttgactga
     9541 tcgtgcaacg gtagataaag cagatacgat gaaggtgacg acttgcctta atcctttgca
     9601 tacgtcttta gctattatgg gttgtttact ggggtatagg agcatagctg aggagatgaa
     9661 agatccagat ttatcgctgc tagtttctga aatcgggtac aatgaaggcc tcccagttgt
     9721 ggagtctcca ggtatcattg atccaaagat ctttctggaa gaagtaatta ctgtgcgctt
     9781 ttctaatcca aatattcctg atatgcctca gcgaatcgcc acggatacct ctcaaaaggt
     9841 ggcgattcgt tatggtgaga cgattaaaaa atatatggaa atagaagtta aagacccgca
     9901 gacacttgta ttcataccat tgactctcgc tgcttggctt cgatacctta ttggcattga
     9961 tgatcaagga aaaccattca tcccgagtcc agacccacta ttgacagact ttcagcataa
    10021 gctttccgat attcaactgg gtacgcaaga tacatcacat attcatgaga aaatggctcc
    10081 tattttgtca aataaagaaa tttttggtgt agatctttac caagcagagc ttggcagaaa
    10141 aatagaagaa atgttcatca agatggtagc aggaccgaat gccgtacgaa aaacattaca
    10201 ggattactta caagaacatg gaggaaattt atcatggaaa tgacttttag atggtacgga
    10261 tcacaggaag aaaagattac cttagatgaa atcagacaga ttcctgcggt caaagggatt
    10321 gtaggcacat tgatggatca caggaagaaa agattacctt agatgaaatc agacagattc
    10381 ctgcggtcaa agggattgta ggcacattga tggatatacc agtaggagag gtatggccca
    10441 gggagaggat caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa
    10501 ttgaaagcgt caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata
    10561 tcgaaaacta taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct
    10621 ataattttat gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg
    10681 gctcatccac gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg
    10741 cgcgcatcga acattccagc aatgaatttg aattgccggg atgggaacct gagcgcttag
    10801 ctcatatcaa atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa
    10861 attttgccta ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg
    10921 ctgtacatcc agatgatccg ccatactcga tttttggatt acctcgaatc gtaaagaatc
    10981 gagaagattt agattggttg tgccgcatag tagacagccc ttataacgga atcactcttt
    11041 gtacagggag tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt
    11101 gtcaaagaga tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag
    11161 atttctatga ggcaccgcat aaatccgaat acggttcatt agacatgtac cgcatactaa
    11221 aaacaatgta tgacaacgga tttaacgaat acatccgtcc tgatcatgga agaatgatct
    11281 ggaatgagac aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc
    11341 taaatggatt atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa
    11401 aactgtaatt ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga
    11461 ggttgttaat aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt
    11521 cttttttcga ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc
    11581 tcataatcac ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt
    11641 tgatatgaga aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca
    11701 taaaatcatt ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca
    11761 gatctttact gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt
    11821 ttttgtagcg atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt
    11881 ctaaaataac tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa
    11941 tggcggtttg gacttctttc aacacgctat gttcttgata aagcatgaaa gtaggaaggg
    12001 gcgtatttgg ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca
    12061 atgagtaaag aatcttttct ttttcaggta taaacaaatc aaatacagta caaaaggact
    12121 ccaccatttc tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt
    12181 gctccaagct tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca
    12241 ctaagatgcg ttgttcgcga caaattggca gattaggtcg caaaatatct acagagggat
    12301 caataaaaaa gtcaatatat tcatactctg tgaattcttt tcgtacgtct ttcacagaag
    12361 ttgcaggaaa attctcttta catattttta atagaaaata taatttatct atcataccgc
    12421 tatttagtcg gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt
    12481 gcggatcttc atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta
    12541 atgctgcata ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa
    12601 aggagataga caaaccgtac aatgctaaaa atgcattaat ctttttcatt ttcgtataca
    12661 aataagaggc actatagttt gtttgctgac ttaaactgat aatcgaattt gtctgacagc
    12721 tgaacaaaga agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac
    12781 gataatagca gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct
    12841 tatcaatatg cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt
    12901 atagtaaaaa atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa
    12961 agaaacgatg ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca
    13021 agagaaacgt tttaatcgct tcttgagttt gcatgaaaga ggctcctttt tacttaaatt
    13081 gttaaaactt gaaaatatta ctgttttttt cataaattta gacaaaaaaa cagtaaacat
    13141 ataggtagaa tctctactat cttatttctt gcatacaaaa aaatcaagga gtgagagagt
    13201 tatgagatat tcaaccatct taatatcaac atttgcagcc ttaagcttat cgcttactat
    13261 aaatcagaca gtccacacag aggagataga aacagccaaa tggacagaaa ccaagtgggc
    13321 caacgtcaat cgaattacct ttcaggatga attttcatcc ttcaattcag gagtcgcaaa
    13381 attaagaaaa ggacaaacag cgaccatcaa agcaaaaatt gactattcag gagacttagc
    13441 acctatacta caatcagctg ttacatttga gaatgttgat cccgctttgt cgattggcta
    13501 tgacgagaac actttgacta tccaacacaa gcaagcgata tttaccttca ccgttacatt
    13561 aaatcaggac ttgttaaaac cagcgctttt tactgtcaag gtatcagatg gactaccgaa
    13621 cgatcatcat catttaacgc cgtatagtca gcggcagaca attgagcaag attcagctat
    13681 cgactcagga ggagatcttg ttgaagaacc aacagataaa ccagaaaatg aaaataagcc
    13741 agaagtaccg cctacagaaa atccagacgg agaacagaaa ccggaaatag aacctggaga
    13801 agaacccgat acagaaactc aaccagagcc agacaatgaa tcaaaacctg aaataacccc
    13861 tggagaaaaa ccagatgtag acccagaaga aaaaccagat gtgactccag aaccagatac
    13921 ggattctgga aaccaaacag taccggaaac aaatccagac acagataatg aaactgaaaa
    13981 tccagaaaaa ccagaggtag ccccagaaga aaaaccagat gtaactccag aaccagatac
    14041 ggattctgga aaccaaacag taccggaaac aaatccagac acagataatg aaactgaaaa
    14101 tccagaaaaa ccagaggtag atccagaaga aaaaccagat gtgactccag aaccagatac
    14161 ggacgcaaga gaccaaggaa taccagaaaa aataaacaaa aaaacaattc aagaggatgg
    14221 aaaaaaagag agtaaaaaat caaatctagc aattctaaaa ataaatgaag agcagctgaa
    14281 taaaaaatca cgaatatttg atagcgccca atcagctgaa accttaaaaa gtagcaagga
    14341 tacaaccttt gctgtcctga aacgaaaaat aaacagctac caaaatccgg agagtcacaa
    14401 aacaaagtta tactgtggag cggaatcatt ctattaagta tagcaacaat gctttctgca
    14461 aaaagattta aacaaaacag atctctctaa catttgaata atgtctgtga acgcattttt
    14521 caaataaaaa gaaatatcca gaaaatgacc ccaaaatacc agattgaaat ctgatatttg
    14581 gggtcgttca tactttatct ttcttcatat ttattttaag ggatatctgt ttcttttgga
    14641 agatggttcg ggatcttttt cactttcaat acatctaaaa agaaggtaaa gatcggaatg
    14701 ccgacaatca agccccaaac gccaaagaac cgttcgctaa caagtaagac cacaaatgta
    14761 taaaaaatcg gcagttctgt tttgctggac atcaacttcg gattcagcac ataagactca
    14821 attagatgga caatcaaaat cgtaatcagg atataaacaa catctcggat gcctccttgg
    14881 gaataagcta tgaaagatag aggaatacaa gaaatgataa cacctgctac tgggatcaga
    14941 cttaaaatga aaatcatgat cgctaaactc agcagctgat gaaagccaaa tacagccaag
    15001 gcgattgtag tgagcaacgt atttaaaaga gcgacaacaa attgagcttc taataccaaa
    15061 ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat cttgaaagaa ccaagaaaat
    15121 tcacttttta aaaacaatct tgagaataca attgtttttt tcttttcaat cataaaaaag
    15181 aaacttaaga tgaatgacat agcaaacgcc actgccattt ttcctatatc ctgtaaatag
    15241 ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa aatcgtttcg ctcaatataa
    15301 gtatggatat attgtaaaac aggatctgca tcttttggct gatgctcgta aaaattaata
    15361 acggactgga tcatgtcaaa tgtttgatga ataagcatcg ggatatactt tgtgaatgcc
    15421 agatagataa ggtaaacgat cagtgtataa atcatgatag ctaaaacacc tgtagggatt
    15481 cggacaaatc gctgaatgat ttgaacaaga tgaatcatca aataagtaaa gataaatgtg
    15541 agcaaaatgg ttgtaatcat acttctaact aagaaaagaa caaaaacgac aaataataaa
    15601 acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct gataaacggt caaatcgaca
    15661 acctccaaat aacaaaaaat aaacacgtta ttcattatat gatggaatat gaaaataaga
    15721 aagagcaaaa agaaaaaccg ctaaatgacg ttatcttatg agttggtttt cctcataaaa
    15781 tcgttcatct agcagtttca gccctttttc tggtcttgat gaaaattatc tagcattttt
    15841 cgggtcagat gttgcagtgt atccaattct tcctgttcaa gtggaagata agacttgatc
    15901 ttttgtggaa taccttctgc ttgtttttgc aacgcagcac cttgagcagt cagcgtaaca
    15961 tttacgattc tttcatcacc cgtttcacgc tgacgattaa tataattttt ttctgctaac
    16021 ttttttagta agggagtcag cgtaccagaa tccagacaca aatcatttcc tagctctttc
    16081 actgaagtct ttttcttttc ccataaaact aacatcacaa gatactgggt ataggtaaga
    16141 ttcagcggct tgaggagggg agtgtagtgt ctaatgattt ctttagaggc ggcatataaa
    16201 ggaaaacata cttgattaac taaatataga tcttccattt ttccacctcc gctgttttac
    16261 tatagtgtat catagcggat ttacacaaaa aagtctattc actaactgct ttcagagtaa
    16321 cttctatatt tccagctgta gcttttgaat acggacacac tttatgcgct gcctctaata
    16381 attcttgcgt ttcttccaga gaaagacctt cgatagatcc ttcaatctct acacctaaga
    16441 caaagctcgt ttcactttct gaataaaggg aaacttgtgc actgacagta gaagccccag
    16501 agatattttt ttgctgtttg atcaattcta atgcactgtt aaaacatgca ctgaaaccag
    16561 ctgcaaacaa ttgttcaggg ttggttgcat tttcgacgcg tttaccagga gctgcaatat
    16621 tcaattcgaa tgtacgatcg ggattatgca cttctccaga gcgtccgcct gtgttgatca
    16681 ttttcgtaga atacattttt ttcataaaat taccaccttt aattatattt tatacaattg
    16741 aattttacac aatataattg tggaaagcaa agaaatggat tggcataaga taaataagag
    16801 aaagcacatg aaacagacgc gctttctcga atagcaaaca tatacaaaaa atatcagaga
    16861 caaaaaataa tctcatcata aacattacat atatttctca aaaagttgtt ttctttcttc
    16921 aaaaattact tcacgatgga cctgatgaag catccataaa taaccaaaag gatctctgaa
    16981 cattgagtta gtcacaccaa agtccatcat ttctgtcatt tcttgaatct ctacacagcc
    17041 atttcgtaat gctttattat gtgtgtcctc gatatcagag acgatgatat tgaaccagat
    17101 cgtatttgga tgttcagcag taggagcagt caaaccaaga gacgtgtttt catccagcat
    17161 atggaatctc gtaccataca aggtaaaaat gacttcatta ttgcctctag ggtaatctgt
    17221 tgcctcaatc agttgtattt catcaaagat tgtttgatac agttctagtg ctttcaaact
    17281 atcatttaat acaaaatcga tttccgttcc tttatgcata aagatctcct ctccttttca
    17341 gtcgaacagt tactagattc ttttattata ccagctaaag aaaacgcatt caaaaaaata
    17401 gcggatttac ttaccaactc attcgattgg atgataagta aatccgcgtc ccattacttc
    17461 tgctacgctg ctgacaatga caaaggcact tggatcgatt tcttctatca atggtttcat
    17521 tcgggaaaat tcatgatcag tcacaacgat caataaaacc tcttttgact ttcgattata
    17581 cccgcctttt gcctctagca gggtaaggcc acgtccaatc tcagcactta agcgaaggcg
    17641 gatgtcttcc aaataatgtt cactcatgac cataacagat ttctttcgat tgatacctgt
    17701 ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat aaagaaactc
    17761 ttctattcca aaaataaaca gattaagaga aacaacgaat gcgtctgtaa aaaataagcc
    17821 aatcgaagta ttcaagtgga aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc
    17881 gctagaagag ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa
    17941 aatgacagac aacaaccgat ctgaagtaat cattgtttct ggaactattg caatgattaa
    18001 aggaaaaacc aagcttccga aaagtacttt aaaaaagggt tttttaccta aaaatagaag
    18061 agctagtacc aacataacga tattcaacac taaaataact atagctcggt caaagcctaa
    18121 agcagcttcc agtaatattc ctagaccgct gacacctcca gcagcaatat gatgaggccc
    18181 taaaaacata ttgatagcga ctcctaatat gaagagggca aaaataatct taatgtatgt
    18241 ccatataaat tgtctcgttt ctttcattta ttcacttcct taattcatta ataatactct
    18301 tatcttatta taacttactt ttcaccttaa gttaacttta attaataaaa aaattaggct
    18361 agtctaaatt attatgtttc attcttctag aatatacttc tacgccaaaa actgttaaaa
    18421 aacttttact ttagaaaatt ggagaaattg atcgcttaag ataaattcag gacactaaat
    18481 agtacaggaa gtagactaaa acttttaaaa gttttaagtc tatctatttg gcaagtttaa
    18541 aaaataaaaa ttcggtatca aaaaactgtt accgataaca tttttgaaag cgttgtcttt
    18601 tgcttctcta tcacttatag tgatgacgtc atattaaaac acaaggagga aatcaatgga
    18661 tacagctgca atttatcatc gaccggaaag tgaatatgca tttctatata cgaatacaag
    18721 atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg tgctatgtgg
    18781 agatccatat accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg
    18841 tctaagtacc aatgttcatg attattggga aattgaagtg acgagcgaaa caagtcgctt
    18901 acagtatgcg ttccatgttg ttggggaaga taacacggac tgtttttacg gtgatcaagg
    18961 aatttttcct tatcgagaaa atgtgattac tgaaccaaat ttttattttc gaatacctta
    19021 ttttcatcaa atcgatcgat tcactattcc agaatgggta aaagaaacag tatggtatca
    19081 aatttttccc gaaagatttg caaacgggga caaagcaaat gaccccgaac acactttacc
    19141 ttggggaagt aaagatccag accgagaaga ctttttcggg ggagatttgc aaggtgtgat
    19201 ggatcatttg gattacttga cggaccttgg catcaatggc atctattttt gtcccatatt
    19261 taaagccaca tccaatcata aatacgatac gattgattat tatgaggtgg atcccgcttt
    19321 tggcgataaa aaacttttaa aaaacttgat tgatgaagca cataaaagag ggatacgtat
    19381 catgttcgat gctgtattca atcatatggg tgtccattca ccacagtggc aagacgttct
    19441 caaaaacgga gaaaaatcga tctataaaga ctggttccac atccgttttt tcccagtaga
    19501 ttcttatcag atgactgact taccagaaac tgctgaaaat atcccatatg atacttttgc
    19561 ttttactcca ttcatgccga aactaaatac agcaaatcca gaagtgcaaa aatatttgct
    19621 ggatatcgca acttattgga taaaagaatt cgatattgat ggatggcgtc ttgatgttgc
    19681 taatgaagtc gatcatcatt tttggaaaaa attcagagaa gcagttacag aaatcaaacc
    19741 agacatctat atccttggag agatttggca ttcctctcaa gcttggctgc aaggggatga
    19801 attccatgca gtgatgaatt atgcatttac agattcgatt aaagattatt ttgcaaaaaa
    19861 gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc tttacagaga
    19921 tcaggtgaac gaaggaacat tcaacttatt agattctcat gatactgcca ggatcttgac
    19981 cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa
    20041 agggtctcct tgtatctatt acgggacaga aatagggatg actggtgaag atgacccgga
    20101 ttgccgcaaa tgtatgatat gggagaagga ggaacaagat ctagagctat tcggttttat
    20161 aaaagaactt gtttctctaa ggaaacagtt gtctaagatt atttctgaag gttctactca
    20221 gtggctaata gtaaatgatc gtgaagataa gctctatttc acacggaaac tagagggaca
    20281 gatcatctat gtttacttta atcagtcaaa agaaccttgg gtagtagaac aggaaaatga
    20341 ggtgattctt agtcaaaatt gtcagttgtt agaagacggg aaagcagaga tacgacaata
    20401 cggatttttg atcatgtgtt gaaaactgtt accggtaaca acaagaaagc gtttgcaaat
    20461 gaagatttgt gcccattata ataagggtgt aacagcacgt tgaatacaaa ggaggaaatg
    20521 aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt taatctcttt aggaatctta
    20581 ggcggattag cggcttgtgg caatggtaac tcttcagcag aaggggaagc cgatgaagga
    20641 aaaacgttaa cagtttccgt tgattctgga tataaagatt atataaacga aataaaaaac
    20701 actttcgaaa aagaaaacga tgtgaaaatc aagctagtag aaaaggacat gttcgatcag
    20761 ttggaatcat tagcgttaga tgggcctgct ggaaagggac ctgacgttat gatggcagca
    20821 tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa actgggaaat
    20881 gaagcagctt acgatgaaac agacaaagcc caagtgactt atgatggaaa aatctacgga
    20941 gaacctgcag taatcgagac ccttgttttg tactataaca aagatttagt cgatacagca
    21001 ccatcaacgt ttaaagattt ggagaatcta tccaaagaca gtcgttttgc ttttgagtca
    21061 gaagcaggaa aaaatacagg atttttagct aaatggactg acttttatta ttcctatggt
    21121 ttaattgcag gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc
    21181 ggtttgaata atgcaggtgc tgtagaagga atctcttatg caacagattg gttcaaaaat
    21241 gtttggccaa agggaatgca agacataaaa agtgcaggag acttcgccag ccagcaattc
    21301 atgtccaata aaactgcagc aatcattgac ggtccatggc aagcgcaaac atacaaagaa
    21361 aacaatatca attatggtgt agccaaaatt cctactctaa ataatggaca gccttatcaa
    21421 ccgtttggcg gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa agacctctct
    21481 caaaaatggc ttgattatgt aacgaatcag gaaaatcaag aaaaattctt tgaaatggta
    21541 aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa cgatgaatta
    21601 acaacagctg tgattgaaca atatgaaaca gctcaagcga tgccaaacat cccagaaatg
    21661 ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca
    21721 ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg aagccataga acaaaaatac
    21781 tgattaaaat gataaccgtc catttttata aaaaatggaa caggtgcttc agataaaagt
    21841 ggcaagagcg agatggaaaa ttatcttgct cttgttgcca ttatcttaaa agcagtttgc
    21901 ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga taaaacaagc aatatcaacg
    21961 aaaaaaaaat cacgctttgt tcacttattc cagggatggg gcagtttgta aatggccaag
    22021 cttttaaagg tatcgttttt ttcgccatat tcgtcctatt cattggtcag atgcttctgg
    22081 gcggatttca tgcattggta ggattggtta ctttgggaag tataccaatg gaagatcatt
    22141 cattatttat tttgattgaa ggcactttgc aattcatcat tacaatcatt ttcttgattt
    22201 tttacggttt gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa
    22261 aaatcaatcg gacgatgaag gaagtcatac ataatatagg tgatgaaggt tttccttatc
    22321 tattgacact tcccgcttat cttctcatga tttttacgat cattttccca gtcctagtta
    22381 cattgttcac tgcatttact aattatgact tcaatcatat tccaccagcc agtttaattg
    22441 attgggtagg ggctaaaaac ttttttagta tcttcttttt aagctcatat cgtaatactt
    22501 tttttgcagt cttttcgtgg accgtgattt ggactttatg tgcaacaact ttgcaaatca
    22561 cccttggtat tctcacagcg gttgttgctc accagaaatt tattaaattc aaacgtattt
    22621 tcggtatcat tttcttatta ccatgggcgg tcccagcttt tattactatc atgagttttt
    22681 cgaatatatt caacgatagc gtaggtgcca tcaatactca agtgattcct ttcataaacc
    22741 agcttccttt tgtagatatc agtgcaatct cttggaaaac ggacccgaac tggacgaaag
    22801 tagcaattat cttagtgcag ggctggttag gatttcccta tatctatgtg atggttacag
    22861 gaatcttaca atcgatttca gaagacttat atgaagcagc aaaaatcgac ggagcaaatg
    22921 catggcagcg cttttcaaac atcacgcttc ctgctgtctt cctagtggca gcgccaacat
    22981 ttattacaca gtatacgggg aatttcaata atttctccat gatctatctg tttaacaatg
    23041 gcggtcccgg aagcgtaggc ggaggagctg gttctactga tattcttatt tcctggatct
    23101 ataaacttac gactggaact tctccgcagt attctatggc ttcagcaatc acgttgatta
    23161 tctccgtcat cgttatctgt gtgtcattgc tggtatttaa aaagacaaat gcgttcaata
    23221 tgggggatta acaatgaaaa aatttcattc gagtgctcga ttccaaaaac gactttctca
    23281 cttttttact tacttttttc ttttaatact gtcagtcatt atcatctatc cgcttttgat
    23341 cacagcaagt actgccttca agtcaggaaa tattgcagct tttagtttag atctttcaag
    23401 tgactggact ttaggaaact ttactcggct attcagtgaa acactttacg ggacttggta
    23461 taaaaataca ttgatcattt ctgtcctaac aatgatcgta caagtgactg tagtaacttt
    23521 ggcgggctat acttatagtc gctatcgttt tatcggccgc aaatcaagtt tgatgttctt
    23581 tttagtgatt cagatggtac cgactatggc agcattgact gccttttatg tcatggcttt
    23641 cttattagaa gcgctcgata agtactggtt cttgacaatg atctatatag gcggtggaat
    23701 tccaatgaat acttggctga tgaaaggata ctttgacact gtaccaatag atttagatga
    23761 atctgctaaa cttgatgggg cagggcattt caggattttt ttccagattg tattaccgct
    23821 tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt atgggacctt ttggggattt
    23881 catgttagct aagttcctgc ttcgaacacc tgaaaatcag actgttgctg tcggcttaca
    23941 aacatttatt tctgatgtga caaaccaaag agtctctctt tttgctgctg gggcaatttt
    24001 gattgcacta ccgatttgtc tattattctt ctttttacaa aagaactttg ttaccggcct
    24061 gacatctggc ggaacaaaag gataggagca gagtatcatg caaacatttc cgttaaacta
    24121 tttttccgca ttacgtacgc caactcaagt gtttgcggga cgcaaactat tatcatggcc
    24181 taaatttttc ttgatatttg tatttttagt ttccttgatg gtcatgcctg tgactctttt
    24241 ttatgccaat cagatccaag cgattccatt agaacaattt ctatccgttc attcgctgat
    24301 cgatgaacaa ggaacccaaa aattttctga acttgaatta tctgaaactg ggttacaagc
    24361 atcgcaacaa acgatagctg tgacccctga aattttggta ggtgtgtctt tatctgagaa
    24421 acaacaatcc gatcatggaa cttttattga ttttgagaag gaacaatggg tgatccaaca
    24481 aaaggataaa tctggtatac gccgctatac gatgaattat agcccatctt tccaaccaga
    24541 ttctgtacgt acgcccgaag atttccaacg atttttagaa agagaatttt atgccagtaa
    24601 tcgtccaaca atcattttat cttatagttt aagtcttggt cttgtcctat ttgtcatgac
    24661 gagtctcatt ttattcgggg cttccttttt cttgtggatg acaagaaaaa gccaactttc
    24721 atccattcat acattcaaag agagtgcaaa tttgatgctg aatgttatgg gaataggttc
    24781 catggcagca gcagttgtag ggtttatcca ctttgacttt atcttgatgc taagtgtcca
    24841 aacaacgata actgtcttgc tgcttttatg ggtttttgtc aaaacaagat tcaaagataa
    24901 gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa aaatgaaaaa
    24961 taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt cttttctttt
    25021 ttagtattaa cgtaataggg tagttttttt aaggaggaaa atcatgttag agaaaacttt
    25081 atacaatcaa ttattttcac gttctttctc gctccctgtc gaagtaaatt attgggacgg
    25141 tacaacgaaa caatacggag atacggacac tcctccccaa atcaaaatca atatccatga
    25201 agaaatccca atgaaagaga ttacaagtaa cgcttctttg gcactaggag aagcctatat
    25261 ggatcatcga atcgagatcg agggaagtat ccaagcattg atcaacgatg cttatcagca
    25321 agcaaacagc tttttacgag gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca
    25381 tacgaaacaa aaaaacaaag aagatatcca tgcgcattat gacttaggta acgactttta
    25441 taaactttgg ctagatccaa ctttgactta ttcttgtgct tatttcaata cacctgaaga
    25501 tacgttggaa caagcacaaa tcaataaagt ccatcatatc ttagacaaac tctttatcaa
    25561 agaaggcgaa acgttgcttg atatcggttg tggctgggga acattgatgt tcactgctac
    25621 caaagagtat aatgtcaagg caactgggat cacactaagt gaagaacaat acgatttgat
    25681 taccaaaaga ataaaagaag aacatttaga agataaatgc cgtgtattat taatggatta
    25741 ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt
    25801 aggttctgaa aatcttgaag gttatttcaa agtggtaaaa gatttattga aaccaaaagg
    25861 aacagctttg atccatggaa tcagccgaca acaaggcggt gcgacaaatg cttggatcaa
    25921 taaatatatt ttccctggcg gttatgtccc tggtgttact gaattagttg ataatattac
    25981 aaaaaacgat ttgcaactga ttgatttgga aagcttacgt cgtgactacc aattgacatt
    26041 agaacattgg acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga
    26101 ttccttctat cgaatgtggg atctatattt acaagcttgt gcagcttcat ttgaatcaag
    26161 taatatcgat gtgattcaat atttgcttgt tcaacctgga aacaatgaca tcccaatgca
    26221 tcgtgcataa ttgaggcaaa agtactgttt aaagactatt taaaaagaag ctaagtttta
    26281 tgatgctcca gaagtgaact tttttgtcta acttctagga ttgtctctaa agcttagttt
    26341 ctttttcttt atattttcat aataaaagtt tggttcttta atttttaaac atgacagaaa
    26401 attttttctt cgttatactt gtaatagaat actattttat aacaaagggg atcgaatcaa
    26461 tgaaaaaaac tgttatttat actagtttat ttgcagcttt gtttttagct gcttgtcaat
    26521 caaataccaa tcaaggcaca tctgggaata cgtcttccag tagcgaacag cgctcttcac
    26581 aggtatcgga ctcctcaaca aaagcaagca catcttcgtc tagccagaca aaaagttcta
    26641 gtgaagcaag tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc gaattgttcc
    26701 aagataaata cccagatact gcaattacta gtttggaatt agatagtgat tggggatctt
    26761 acttctacaa gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga attgatgctc
    26821 aaaatgaaaa aatggagaca gagaatccag aaactctaga tcgagatgaa caaaatggtc
    26881 aaaagaaaaa agaagatggt ctggatgttg ataaattgat cacgattgag gaagcagcaa
    26941 aaatcgccgt cgacaaagta ggaagcggta cggcaacaga ttgggatttg gataaagatt
    27001 taggtactac ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca tcagtcaaga
    27061 tcaatagcca aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa tttcttcgga
    27121 aaacaagagt tgatttttct tttagtcatg atacactaga caaaataact tgtttgaagg
    27181 agcgatttac ttgagtttag aaacgacctt tgccaatcat atctttgcca atcctttgat
    27241 gaatgcatct ggtgtccact gcatgactac ccaggaatta gatgaactgg ctcattcaga
    27301 agcaggagca tttatcacta agagctgtac gatcaatgaa cgaaaaggaa atcccgaacc
    27361 acgctatttt gatgttcctt tagggagtat caattctatg ggacttccaa acttaggttt
    27421 ttcttattat ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc aaaatcaacc
    27481 gttatttttt tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc ttgagaaaat
    27541 agaaaaaagt gggtttaatg ggatcactga attaaattta tcttgtccca atgtgcctgg
    27601 aaaaccacaa ctagcttacg attttgaggc cacttatgaa acactgaaag aagtattcag
    27661 catcttttct aagccgctag gaatcaaatt gcctccttat tttgattttg ctcattttga
    27721 tcagatggct gatatcttga atcagtttcc attgacctat gtcaatgcta tcaacagtgt
    27781 gggcaatggt ttgtatatcg atacagaaca agaagcagta gtcatcaaac caaaagaagg
    27841 gttcggcggc attggcggag agtatatcaa gccgactgct ttggcgaatg ttcgcgcttt
    27901 ttatacacgt ttgaaaccag aaatccaaat cattggtaca ggtgggattc ggacaggaca
    27961 agatgcattt gaacatctgc tatgtggcgc aagcatgtta cagattggta ccgagcttca
    28021 taaagaaggg ccggaaattt tttctagaat cataaaagaa ttaacgcaga ttatgtcaga
    28081 aaaaggatat acgtcaattg acgaatttaa aggcaaatta cgcaccattt catagcaaag
    28141 agacgaaggt catgattgat cttcgtcttt ttttataaaa aacactcgtc tgttcattcc
    28201 tgtgcaagcg tactcattgt atgttagacg aacttgtggg aaaataacag aggcaaaaag
    28261 gaggataata tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa agaatttagt
    28321 tttcaagaac tagttacgga tatacagaaa aaaataaaga agcaaaatca tgaattgaat
    28381 gcgtttgtcg cgcttgaacc attcgagagt aattcccacg agctattgat tccccagtca
    28441 gaaaaagggc cattggctgg tatcccattt cctttaaaaa tgctcggtca agaaaaaaag
    28501 ggatggctag ctacttcagg atctcgttta tttgaaacac accgagcttc tcatacatct
    28561 aattatgtgc ggcaagcaga agcgataggt ctagttcctt ttggacaaac caatgcgcca
    28621 gagtttggtt tcaaaaatat tacagaccca gtcatctatg gcccagcaag aaacccttgg
    28681 aaccttgaat gtacaccggg cggatcaagt ggaggagctg ctgctgcagt ttcatcagga
    28741 atcgttcctt tagctggtgc tagtgatggc ggtggttcta ttagaatacc cgcttcgttt
    28801 tgcggattga ttgggctgaa accttcgcga gggacgatgc cagttggtcc atatgcttgg
    28861 agaggatggc aaggagctgc tattgatttc ggcttgacag tttctatgag agacacggag
    28921 gctttatttt atgggatgag aagtatccat tccggtgcgc catatcaagt atcgccagtt
    28981 gagtggcaaa cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt tgattcaccg
    29041 atccactcac agattagtga agaggcagta cttgctgtaa aaaaagctgt agatttcctt
    29101 ggaaaagaag ggcatgattt gacagagatt ccttacccac tagatggtcg taaactgatc
    29161 acgtcttatt atcagatgaa tggcgcagaa acagcagcaa tgatccgctc gattgaaaca
    29221 ggcatccagc gagctgtcag aaaagaagaa cttgaaccca tttcttggac tttgttccaa
    29281 tatggtcaga agctctctgc cgctacttac atcgattctc tgcatgtatg ggatgaagca
    29341 gcaattgtca tggaaaacct acatcaagaa tacgatctat tcctttcacc aacgactgca
    29401 tttaccgccc ctaaaatcag cgaagatttg caaagtgatg cgataagagc acagatggaa
    29461 cacatcagtg attgttctga agtagaaggt ttagacgttg tttcttccat gtttgaggag
    29521 agcttgaaga tcacacctta tacacagttg gctaatctga ctggacaacc agcaatcagc
    29581 ttgcctactc atttgacaaa agaaaacatg cctctaggaa tccaatttat ggctgccaga
    29641 ggaagagaag atttgctatt tcaaatcggc tatctatttg aagcaaatca tcagttccat
    29701 ttaccaaatc catggaaata gcagaaacaa ggagaagaag aatgaagaaa aaaagattgt
    29761 ttgctctaat aactagtgcc ttttttattt taagcgcttg cggtacagct gccgctaatc
    29821 aggaagataa cagctggagt aatgtagaaa aaaagaaaga attagtagta gcgacttctg
    29881 gcacactgtt tcccgcatct tattataatg atcaaaatga attagttggt tatgatgtag
    29941 atttggcaaa agaggtggct aaacggttaa atgtcaaaat ctcctttaaa gaatacaacg
    30001 tggatggaca aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg aacgattttg
    30061 gtatcacaaa agaaagagaa aaaaaattct ccctttcaga gactttcaaa tattcatttg
    30121 acagtatgat cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa gatcttaaag
    30181 ggaaaaaagc agctggcgaa cccaatacta gctatatgcg tttagcggaa aaacacggag
    30241 ctaaattagt cacatatgac aatgccacga atgatcagta tttaagcgat gtagcaaatg
    30301 gtcgaacaga tgttattcta aatgattatt atctgcagaa aatggccgta tctgctttac
    30361 cagatatccc tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa agtggttttt
    30421 tattgaaaga aggaaatgat gctctgaaaa agaaaatcga tggcgtatta aaagagatga
    30481 aagaagatgg gacgacaaag aaaatatctg aaaaatattt tcaaactgat gtttcagtca
    30541 aaccgaaaga aacgatggct gagtcggtga gtgagtgatg tactttcctc taattgattt
    30601 ttccctcatg tggacttcac ttcctttcgt actgcaaggc ttggtataca cattagggat
    30661 tggcttcgtg tcgtttcttt taggaaatct ggtagggttg cttttgacag ttttaggtct
    30721 tttggattgg ctgcctctga atgtatttat tcgtttttat ttatcgttct ttagaggcat
    30781 tcccgcacta gtcctgctgt ttctgttata ttttgggtta ccttatcaat taagtgcctt
    30841 aacggcttca gtcatctgct ttacgattac cagcagtgca tttattggtg aaatctatcg
    30901 aggatcacta gcaggcgttt cttcaggtca gtgggacgct ggtttagctt ctggtttttc
    30961 tttctttggg gtattgaaat atatcgtttt gccgcaagct tttcgaatct cagtaccggc
    31021 actcagtaac gtagctatgg atctagtgaa aggtacttcg ttagcagcaa caatcacagt
    31081 gccagaaata tttcaaaaag caaagatcat aggcggtcgg acattcgatt atttttctct
    31141 ctatatttta gttgcactga tttattgggt catttgtatc gggatcgaaa aagtgcaaca
    31201 gtatttagaa aaacgttcta tgcatcagtc aatctgaaaa atagaaatgg tgctagcgtg
    31261 gctggcacca tttctatttt ttgtttcttg tatattctgc ctagtatttt aaaaccattt
    31321 catgtaaaat gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac cccgctaacc
    31381 atcgaaatag aatcctcgtt ataccaatat tgcatcgagc aaggcggctt ggttgtagaa
    31441 gaagtcacga tgcctgatga ccaaggaatt gtagatacgc ttagctgctt gacaaaattt
    31501 gatggaagcc gagaatggcg atgctatgag ctgaaagtga caaaagcaga ttttcgctct
    31561 acagccaaac tgtcatttgt tggacattat aattattttg ttttaccaag taaactatat
    31621 gaagaagtaa aaactgaaat ccctacagga ataggtgttt tagtgtatcg tccgtacacg
    31681 gttgaagagg agatgcctgc ctctggtaca tttctgattg cgaaaaaagc agcaaagaaa
    31741 gagcttgctg taccggaaga agctttgaca aaccgcttca tggcttcttt attcagggaa
    31801 gtaagaaaag caaagcaaat ggagcgaggc gtaaaatatt ttccaacaga tcaattgtac
    31861 aaagaattga aaagacgttc agaaaatcaa gatccatttt ctaacgaacg atattatgaa
    31921 cggtttttag aggaaatagc tagtcaggca atcaatgagt tgcaagaaga agttatcgcg
    31981 cttcagcatg attatgaata tttgaggcaa caacagcaga tccgtcggtt gcctacagag
    32041 ccattggagt gaaaaatatg tactatcctt atttaagagg aaaacaattt gacctattag
    32101 cattaaaaga agcactgtcg cgaggattgc tctccaataa aatccaacca gtgatcgaac
    32161 cagtacgtga ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa aagcaccatc
    32221 caatcgctgt gatccaaaat ccccaagtag gacaattcaa attattcgat cagcatgtgc
    32281 acagttggac agtcaaagaa aatagctctg tcgtccctgc acagatctta acgccagaaa
    32341 atctcagtga agtactggat tcacctccag cgtttcttgt tttcgatgga cagcactacc
    32401 caaaagatac agaggtgtgg aaacagttgg caggagttga ttcgaaattt ttgataccag
    32461 atactagtcg ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt gactcttttc
    32521 aaacgagaaa gcatgttgaa agttatgcag ataaaacaga tgactttttt agtgatgact
    32581 atcttttttt ccacgcagac ggatatattg gattttctga tttcacgatc gaaggtagcc
    32641 gttatttcga taaagggttc cctagccgag cgcttgctat ccatctaacg tatattgatg
    32701 cttacggcaa tatacgcgtg aaacattttg tctctgacag taacgactct gctaaagacc
    32761 aagcattgaa gtttcttgaa gcaggagata aaatgaaaaa atggatcatg cgtcaccatc
    32821 atcaactctt gattacttca ggaatgattg aattattggc tctttaccag caacaaaaat
    32881 tccctggact gggagtgttg aaaaagtggt cacttatgca tcatttggaa cttattagtc
    32941 acctgctaga tcatccaaaa gattggctta aatattattc aaaagttcag gaactatatg
    33001 aagtgatcca atgattagat aatgtaaatg aaaaaggaat agaaaagagg aaactggatg
    33061 tatattcggc aaacaaggaa agaagaagta gaagaagtgg tagaaatcat taatgccgca
    33121 gcggatttca ttgcttcgca aggctctcct caatggcaaa acgggcagaa accaacaaaa
    33181 gaaacgattt tgaacgatat tgaaagaaaa gaaagctatg tattaatagt ggaagatcaa
    33241 attgctggaa cagctgcatt agtctcaagc gtggatcccg tttacacaaa aatagatggt
    33301 agttggaaag aatcaactgg tccttattta tcgattcatc gtgtcgcttt agcagaaagt
    33361 actcgaggaa aaaaactagc aaaaccgttc ttagaacatt tgttgacgat tgcagccagc
    33421 aaaaatatcc aagatatccg tatcgatact tatccagcta atgaaccgat ggaaaagacc
    33481 atctattctg ctggattcac ttatcaaggg atgattgaat ttccttttga acacggcgaa
    33541 agaaaagcat atcaaataat catcaattga ctataaaatc atctgagcaa agataaagac
    33601 attaacacta ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca gaagtggcca
    33661 gcttcaaaaa acaagaaaga atttccgaaa atgcttctca aatttttgtg gaattttagc
    33721 gtattttacg aaatagctgc tttctgaaac tcccttcatc gagaggtcgt gacatagctt
    33781 ttgtcacgac ctctcggctt atcctacgag tattattcga tacatttata tcaataatcc
    33841 tttttttatt ttatctgcag cttcttggaa cgctccatcg tttgacacga ctaaaagtga
    33901 ggggatagct ttcaaacgtt tcaactgtgc taaatcttct acataaaccg ccagtctggc
    33961 tgaaatctgt tcagatgaat ctccacgttt tttcaggcgc gattgcaatg tttctttaga
    34021 gatcgtaata aagataggac ggactaccgt cccgaaatac tcatataaat gccaaaaacc
    34081 atttgctgtc aaaacggcgt aacaatttcc tactgtcagt ttctttttaa tttctgcttt
    34141 actggaacca tatttatgtt gagcatattg gtcccactct gcaaactcat ttcccttgat
    34201 catctgattg aatgtattgt cagaaacaaa ataataatcc tcttgatctt tttccccagg
    34261 tcttggtgat cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa tagcttctgc
    34321 gagtgaagtt tttccagaac cagaaggacc gataaataca tagcaataat tcattgtcat
    34381 ttctcctttg atgattgaac aagtgaaaaa gctttggaaa atatttccaa agcttttaaa
    34441 agtaactttg cttttttgta tattatacca gcaagccagt cacaatagca cttaaaaagc
    34501 tgactaaagt cgctgttagc aaaattttca atccgtgttt tgcgacaaga tttccttttt
    34561 cttcatttaa tcccttcatc gcaccagaaa tgatcccgat agaagagaag ttggcaaatg
    34621 aaacgaggaa gacagatata attgctgttg tacgctcagt aaaatggaaa ttaccatttg
    34681 ctaattctgt catagcaaca aattcatttg taaccagttt agtcgccatg atactaccgg
    34741 catccactgc ttctttccat gggattccgc tgatgaaggc aagaggggca aagatgtaac
    34801 ctaggatctc ttggaaagtg atgccgaata tcccgtggaa gatagcattg atcatggcaa
    34861 tcaaagcaac aaaaccgata agcatagcag caaccgtgat cgccacatgg aaaccatcaa
    34921 gaatgtattc tcccaacatt tggaaaaatg tctgttcttt gttttcttca atgatcagct
    34981 catcttcttt ttcttctaat acatagggat ttacaattga agccagaata taaccgccaa
    35041 ataagttcaa aacaagggca gtgatcacat atttagaatc gatcagtgcc atataagatc
    35101 cgacgatcga catagaaact gtagacatag ccgatacact catggtgaac aaacgtttct
    35161 cagataggaa gggtaattcc ttttttatgg aaataaaaac ttccgattgt cctaaaatag
    35221 cagaagctac tccattataa gattccaatt tgcccatccc gttgatttta cttaatccta
    35281 gaccaacagc tttcataaat aatggaagaa tcttgatata gcgtagaata ccgataatag
    35341 cagaaataaa tacaattggc atcaatacgg aaagaaagaa aggtgcactt cctttatttg
    35401 ctacaccggc aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga tgatcaaaca
    35461 tttttgccaa tacagaaaca accgctgtac caaaagttgt acgtaataat acgaaaccaa
    35521 gaacaagctg taaaacgaac atcacaatta gtggtttcaa tttgattgct tttttgttac
    35581 tgctgatcaa aaaagcaagc cctaatgtga caaataaacc aataatacct aataaataac
    35641 tcaaataatc actcctattc caaattccgt tttattttac ctgttttgca aataaaatac
    35701 taatctttag tgaaattttc attacttttt tagagtgaaa cgcattcatt ttccattaga
    35761 aaataaagcg aacaaaataa aaggtttaaa aagtgtaacg tcttagtaaa gtctataaaa
    35821 aaacgaaact atcgattaag ctaatagttt cgttttcaac atttataagt aaactttttc
    35881 tattggtaat atagattttc ggaagggtag acaggtgcgc aggtaatatc gattcctagt
    35941 tttttcaatg tttgttcatc atttttattg accatcacag tagagtgggc ttgtgctccc
    36001 tcaagttcag ctagtttatc ataagcaact tgagcgattg gattcgttac tgcactgatt
    36061 gataaagcga tcaagacttc attgatgctt agtgcatcca aagaggaatg aagtgtcttt
    36121 gatttcaatc cttgtattgt ttcaagaact aatggtgaca ataaaggaat gtcatctgcg
    36181 atatgggcta aatgttttac tgcatttaag ataactgccg ctgacgcatc catcaatgaa
    36241 cttgtacgcc cggtaatgat gacttcatct ggtaattcga ttgcaatcac agcagaagga
    36301 tcttttgtag gactttgcgc ttgaatctta tctgcatagt catgggcagg gactaccacg
    36361 tttcggtctt caggacgtaa accgacttct tccataatca gtttgattcg attaactgct
    36421 tcttcgtcga tcaatccttt tttgaagtcg cattctgttt ggaaataacg acggatgatt
    36481 tcttgtttag aggcttcttg gacaacggta tcatccacga tcccaaatcc tacacgattc
    36541 acacccatgt cagtaggaga ttgataaaca gattctttac cagtaatttt ttcaataata
    36601 cgtttgatta caggaaatgt ctcaacatcc cgattatagt tcacagctac tttattgtag
    36661 gcatcaaagt ggaaggaatc gatcatattg acatctttta gatcgactgt ggctgcttca
    36721 taagcaatgt tcagcggatg tttcaacgga acattccata ctgggaaagt ttcgaacttg
    36781 gagtaaccag ctgcatgtcc tttttggctt tcatgataca gttggttcaa ggaagtagcg
    36841 agctttccac ttcctgggcc aggagctgtc actacaacga ttggtttagt tgtttcaata
    36901 tactcatttt ttccaaaacc atcttctgag acgatttttt ctacgttgac tggatagtct
    36961 tcgatttctg agtgtttata gaccttgatg tttcgtcttt ctagtttatt gataaagact
    37021 ttcgtggcag gctgtccact atagcgagtg ataacgacac tgttgattaa taagccataa
    37081 ccacgtaatt catcgattaa ccgcaagata tccatatcat aagtaatccc ataatcacca
    37141 cggatcttat tacgttcaat atctccagca tagacgcaaa tcagtatttc agcttgatct
    37201 ttcaattttt gcagcaattt gattttcgca tcttcttcaa atcctggaag tacacgtttc
    37261 gcatgcttgt cgtcaactag cttcccacca aattctaaat ataatttgtc atagtggttc
    37321 actcgttcca aaatatacgc tgactgctct tcaatatatt tttctgaatc aaaaccaatt
    37381 tttttcacta ggttccctcc ttaagtacta aagcattttt tcattttttt gaggattcgt
    37441 caactggaag agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc aacttgtcta
    37501 gatagttatt cattggtatg atagtttttg ataaggaagg tgctacatga aaaaaagaca
    37561 gaaaaagaaa aacgcatata aacactatat ccgttcgatt tttaccggtt atgaaagaat
    37621 gcttgaagat cccgaattgg aacaattgac gtttacttat ctaaacgaag aaacgcagct
    37681 aactcgtgat gagcatcagc gcattcattt cacaacaaga gatctgccta gtaaataaaa
    37741 aataagataa aggtcatcgg attcgattct gtcctatcag atggaaagac tagttgatcg
    37801 aaaaaaaggc tgtaccaaat tttggtgcag cctgttttat ggagcattgc ccgctctttt
    37861 ttataacaat tttttacaga aaatggtact attgaagtat taataagtga aaaacagatg
    37921 agtaagagga ggcaagtagt atgaaagagc cagatacaaa tgcaaattat gagccagacg
    37981 aactagagga gacgaatgcg gatcaggata caggacaaga atcgtcttct ttttacgata
    38041 tcaaagaaaa agcaagcatg aatctttccc aattattaga agacttacag gcttttgaaa
    38101 aagctgtccg cgaagaagat atccctgaga tctaccgttt atacaatggc cgattgaatg
    38161 aagaattaaa aagaagctca aatgaaaatc acgagatcga tcaattgctg atgcgtaaga
    38221 tccatgatcg ttttttacag gaatttcctt ttatggaaca agtagaaaac atctcgccaa
    38281 cgatgagcta ttacaaaatc ggcacttatt accatgatcg gcccacggtc ggtattgatg
    38341 caagtctgcc agaaattttt gttttgccta agatcgatga agagtgggag aaatattccc
    38401 aaccggagtc aacggactat gacaaaaaaa tcaatgagct agacgcaaag gtgatcactg
    38461 ctcagacaga aattgaacgt ttagatgaac aaataaaaga aatcaatcga caaatgactg
    38521 atttgaacga caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa attcaagaat
    38581 tagagaagaa gaagcaagta ctatctaatg aaaaattagg ctggcttcct tatattgaga
    38641 cgcctgaaac aatccagcag caaaaagaag cattgaagca agaagcaaga gcagaccagc
    38701 ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag acgctatttc ggtagcaaag
    38761 agggattcgg tcaagcaatc catgaattct tgatgaatta tcttggcaat gagaatccta
    38821 ataatcaatc taatgaagga ggttccgagt atgaatgaac acgatgaagc agaatggaca
    38881 aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca cttcattgga agaacaaaca
    38941 agtaatcctc tggatgacac agaagaaatc cctatgctag aggaaacaaa aatacacgaa
    39001 aaagaaaaag taactccgac gaatacgaat actgcagaaa acacccatgc ttacgaaata
    39061 actgaaaatc ccgggtattt agaaggaaaa gagcaagatc atcaagatat cgtcagtttg
    39121 attgaaacag cggaagcaga attagcggat ttacgtatga gaaaagattt gatcgaatca
    39181 gattttccac aattattaga atcatatgat cgttttatat tgaatgaaga accaatctcc
    39241 acgatcggaa agacaagcct gatcgaatac ttcacgtatg aattactgaa accgttgaat
    39301 gatattggtc tggaacggtc aaattcagct tatgacacat ggaaaacaag acatttttcg
    39361 atcaattata cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt accgaccatc
    39421 gatcaacgat accaagtgga atcgatgagt ctcctaaacg tatcaccaga gacaatggaa
    39481 atcaatgtcc aagatgaccg ggtgctttct ttgatccgac actggtcagt ggatcgagta
    39541 ttctctgcag ggcagatcac gatattcaat cacaaattga atcaattatt agcccatgca
    39601 agaacacttg gttttaccgt taatcagaca ttactagaca atacgaagcc acttcgtttg
    39661 aatcttcaga gcgaatttga attgacgaat gaagtattgg atgatatttt tatcgtggca
    39721 atgaaaaatc catcctacga tatggaaaag atcaacgaaa ctacttatca agtacgatta
    39781 gataaaggac agtctttaac gataacaaca aacgaaaaag atcaaacagt cttgtcgatc
    39841 tcatctggag actacccacg atctgtcatt gatttcttta tcaattacga attcttggtc
    39901 ccattaatgg tgagaaagtc ctgattggat aagttggatt accagatgtc attcagataa
    39961 ttctgttcca aaggacttgc ggcagttatc cagaataatt gtcacaagtc ttttctgtat
    40021 gtactaaatg gacgtgaata acttgctgat gaaggaggga tttcattagg aatgtccaca
    40081 aataaaatag cacagcttag cttattgtct gctgcttgtg tagcaggtag gatcgctttt
    40141 caatttattc cgaatttcca gccaatgact gctatttttt tatttatagt actttactta
    40201 agcttaaaag atgcgttaat tgtcatgagt ctttctattg cgatcagcag tttttatttt
    40261 ggtgttggac catgggtcat cggccaatgg atcagctata ctgttgttct gagtttgttt
    40321 tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg agtctgtttt
    40381 tttctggcgg gcatggttta cggaataggt atgaccgttt ttgatacgct gttatatcgt
    40441 ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat gcacagtata
    40501 ggcaatgttg tgttctttct tgtttttctg ccagtcgtca aacgttttta caatcattca
    40561 ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca atgagtgtca gtatactcat
    40621 tttgacaagt tgcagtacaa cctatacaca atcggcttcc aaccaaaagg aggaagtaaa
    40681 aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt caaaactttt cggaaaaaga
    40741 agtgttcttc aaaaaaggcg atgatttact gagtattttg aaacgaaatt ttgaagtcaa
    40801 agaagataac ggcttcatca cttcgcttga agggcactca caggatgaga aaaatcaact
    40861 ctattggatg tttactgtgg acgggaaaag tgctactact ggtgccaaag agatcaaatt
    40921 gaatgatgga caatcggtca ttttcaattt gtctaaacta taagtttcat aaaaaactgt
    40981 taaagggcga atattgccat aattcagtga gttcagttga tgaaagaagg aaaataacat
    41041 ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct gctctagggg ctaatattct
    41101 agttgctatc agcaaatttg tcggctatgc aatcagtggc agtgccgcca tgctaaacga
    41161 aagcatccac agtatcgttg attgcagcaa tcagattttg ctgttattcg gtgataaaca
    41221 agccaataaa ggacagagcg agttgcatca atttggtgaa ggacgagcaa aatacttttt
    41281 cagtacaatc gtcgcaatga tgcttttctt cggcggtggg gcacttgggg tgatggaagc
    41341 ttttgaaaag ttgacgcatc catcccatga agtgggtaat ccgatcattg ttatagcaat
    41401 actgttattt ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga aagaaatcaa
    41461 agaattgaat actgaaaaac tttccacgat gcgctttttg cgcgaaagtc gacacagtga
    41521 aatactgatt atttttacgg aagacttgtg tgctgtaatc ggtttggtct tggctttagc
    41581 aggaacaata ttgacttatg caaccgggat tgctgcattt gatgcgatca gcggattgct
    41641 gatcggtttc ttgctgatgg gagccgccat tttcctagca aaagaatttt atagtctgat
    41701 catcggtgaa agcgtgacag caagcgattt atcaaaaatc aagttagctt ttgaacgtcc
    41761 tgagatcagt cggctgatcg atataaaaac agttcattta agtccaacgg agattttagt
    41821 tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa gcttactcgg tcatcaatga
    41881 tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag atgtatatat acattgaaac
    41941 agataaatat gacccaaatt attctcgtaa ataaaacgtt tattcatgaa ttgaagcaaa
    42001 aatgagaaaa atataatctt gtttttaaat aaaacggcta caatttagcg aatttatgtt
    42061 acaatgagtc cgctgtttat ttgttgtaat taaaggagat attttaatga acaaaaagaa
    42121 aagtgcgcca tggcttcaag ctttgcatgc agtgatgcca ctttgtataa gctatatacc
    42181 agtaggatta gcttgcggag tattgttgca aaaagttgga tttgatcctc tcttatcagg
    42241 actgttatcg attctggtct tttccggtgg cgcgcagttt ttagttgcat ctatgcttac
    42301 gacgcaagca tcatttgcaa cgacattatt gatggttttc tttttagaat tacgctatac
    42361 attattagga gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt ttttggctgt
    42421 cttttctcaa tcactgaatg atgaaaacta tgcggtgaac tacttaaagt tttcaactga
    42481 cccttcctgg aataaaagaa aggctttata tgtcaattgg ttctcaatga ctgcatgggc
    42541 cggtagcaat atgatcggta attttttcgg ctcggtgatt cgagtagatg ctgaccttgt
    42601 ccattttgct ttgacggcca tgttcatttt catgtttatc atgcagatga aaagcgcact
    42661 actgatcttt acaggtttat tctcaggcgt attaggggtc gtattcatgg tattattcca
    42721 aaatactttc ggattgattg cagcaacagt cattgcttca tttgccggat ttatgctgga
    42781 gaaggcattc aaaaaagaaa aagctaagaa actaagagac cgcggtaaag atgtcaatga
    42841 gccgctcttc aacttcccgg aacaggaggc caatcaccat gactaaacta tatttgctac
    42901 ttttagttgc ttgtctgttc atcgtggcat atatcccgcg cttgttcccg atgctttatt
    42961 ttactcatcg aaaagtgcca tcctggttca gtgactggat gaaatatgta ccggtggcat
    43021 tatttgcggc gttagctttt aaagatgttt tcattactca tgaacatctg gatattgctt
    43081 ggaatatcaa aatagctgca atgattctag tagcaggcgt ggcttacaaa acacgttcga
    43141 tggcgttgtc agttttgaca ggacttgcgt ctgtcttttt gctgtctatg ctttaaactg
    43201 ttttatttta ttaaaaacag agattgcgac ataagttgtt caaactgatc tatccgaact
    43261 gcatttgcaa cagtcaattc tggatgcaag tttattcgta gggataaaaa agattagtgt
    43321 tggcttatga tgcaagctct tttttatttt aggaattaga gagggatttg tccttctatg
    43381 attttttgct ataataggga agatgtttat tttatgaaag aatagttgag ggaatttaaa
    43441 tgaataaaaa attgatgcaa ggaacatttt ggctaacctt tgctaatctt ctatgtaaag
    43501 ttttaggggt agtctatttg attccctggc taagtatgat ggggaacaat caagatggta
    43561 tgcttgctac gacattgtat aatgttggct atcttcctta tggattgttc ctgatgcttg
    43621 gtacagtcgg atttccaaat gcgattgcaa aaaaagtagc tgtagccaca aaagaaggag
    43681 ataacgctgc ttgtcggctg atttttagaa gtacgatcaa tatcatgttt gtcatcggta
    43741 tcgcctctgc tgcactgatg tatttatttg ctcctttact agccggaatc agtccgattg
    43801 ctaacgttaa taatgggata ctagctatca gaagtttatg cccatctttg attgctatcc
    43861 caattcttag tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg ccttatggaa
    43921 cttctttgat tatcgaacag gctgtgcgag tgatcgttat cttggctggt acttattact
    43981 tgcgcgtatt gactgacgga accatattag aagctgtatt gatcagtaca gtcgcttctt
    44041 tcttcggtgg gttagctgcg attatccata tgtatatcgt cggacgcaga aaagacttct
    44101 tcgaacttaa agattttttg atttccagcc gttatttcga aagagaaaac cgatctgcct
    44161 ctgtctctat cattcgggaa acactaccgt ttatttttgt cggctcagtc attactatcg
    44221 ttcaaatgat cgaccaagta acgatgaaac cactgcttca ctttttccgt ccagagatcg
    44281 cagatcagca actggaattc ttattcagcc gtgcttctgt caatccaaat aaattgacat
    44341 tgatcctgat ctccatggta ggaacagtag ctataagcag cttgccaata ttaagcacga
    44401 tgaagaaaaa agatcggata caaatcgaaa aaacagtagg agatagtttt tcgattgcgt
    44461 tattgattct tcttccttca ttgactggaa tgtctttatt agctggacca ctatacacat
    44521 tgttttttgg ctatgatcct gaaagtgtcg gatatttcca aatggctttg ctagcttctt
    44581 tgtttttctc tttattcacg atcctatcta cgatgcttca atctttgaat caccatcgtt
    44641 ttgcgatccg attaactgta gaagcaatta tcctgaaagt catcttccaa gtaatcggat
    44701 taggattagt tggcggatac gggatgagct tatcaaatgg cttagcattc ggtattgtct
    44761 ttgttcgcgg ctatcacttt atgtgcaaag aataccgaat agcaccttta gcaaagatca
    44821 gcaatttttt cttaaaaact ttccgctcca ccctgattat gcttgcagta tgttttgccg
    44881 ttttcttttt gctgaatcag cgattgtcaa tggaatcaaa aagctatgca atgatttatt
    44941 gtgtggctgt aggaagtatt ggcggcttag tatttttatt ttctcaattc ggaaaaaatg
    45001 ggtttcgaat gttgaaaaat gttaaacatc atagcagaag caaagaatag gttaactaat
    45061 cgttttttgc tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc gtttttctat
    45121 tttccaattt tagtttcata tctaatctta cttatgatat agttataagg tctggaaaaa
    45181 ttctttgaaa cgaggaaatt aaacacatga aacttgcagt agttacagat agttcggcat
    45241 atctgccgga acgtatcaag aaccatcccg acttattcgt tattccaatc cctttgatta
    45301 tggatgggaa aatttataac gaaggaatcg atatcgaagc ggatgaatat tatggacttt
    45361 taaaaaacag taaagatttt cctactactt cacaacctgt cgtaggggaa gttttgggtt
    45421 tatatgagga attgaaagag aaagggtatg acacgatttt aagtatccat ctttcttcag
    45481 ggatttctgg ctttgtcaac acattatttg cgatgaaaga tgacatcagc ggagtaacgg
    45541 tcattcctta tgactctaag atcactagta tgccgatggg acacatggta gaagctgcct
    45601 tagacttaaa tgatgaaggt cagtcccttg atgaaatact tgcacatatc gatcgaatcc
    45661 gagataatac ctacgcttat ctgattgtag atgatttgaa caatttagta cgaggaggac
    45721 gtttgacaaa tggtgcagcg ctgattggca gcttattaaa aatcaaaccg atcctgacat
    45781 tttctgaagg gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa gcatttgctc
    45841 gagctgaaga aattattggt agaagaaata acgaaataca accgccagtc aaactttacg
    45901 tgatacatgc aaataattta gaagtagcga ttgcggagaa agagaaattg cagaaaaaat
    45961 atccaaatgc agaaatcgaa attggtcatt tcggaccagt cattggtaca catttaggtg
    46021 aaaaagccat cggcattgcc atttcagcgc aataattcct tttattcaaa aaacatacca
    46081 aaaatattcc aacatcatct tggactttct acatgttact aatgagcgtg taaaagaacg
    46141 agatggtgtt tttatttcta tcaatgaagc tctttttctt tgcaatcctc acttcaatcc
    46201 tctataatgt cgcatatagt ttagtaaatg agtaaactac taatttactt atttactaaa
    46261 aataaaagga tgaggagtta aaataatgga aataccaaca aatttaaaac ataaaccagt
    46321 aatcgttgta gaagactatg ataaagttga tggaagaaat gcattaaata cggatgcaaa
    46381 agggctttca ctgggccttg ctcagtggaa tgatcgtgga aaagtcgata tctcaggcaa
    46441 ggtatggcga catactggag aaaaatggtc acgacagtcg gaagaattac caatcacacg
    46501 tattttagat ttagcgatct tagctgcaca aggaagttta tacttccaag atgcttatcg
    46561 ttatgaaaaa ttttatgatc cagaaaatcc agtcgtagac atcattggat tacaagggaa
    46621 ccgaatgacg gtagaagtag ataccaaaaa tccaaagatc gatgaagaca tcattttgta
    46681 ttacgataca ctacaaaaag acggagaact gattggggaa cgattccgta ttttaaaacg
    46741 tcttttagat gagctaggat attaatagac agagatgatc aaaaggggaa gagaagcatg
    46801 gagaatttct cactaacaga aaaccgaacc ttttcacggc tagaaaagca aatgatctgg
    46861 aagaaaccga aaagccatcg gacaagtgca gaagaatggc ggatcgcctc cgaaatcaaa
    46921 gggaattggc aagatcctga aatgaaaatc agtaacgtac ttttggaagg agatgccggg
    46981 tcggggaaga cacaattagc caaagcacta tctgctgatc tgcaacttcc ctatacaaaa
    47041 gtgacctgtt ttgcagatat ggacaaatca gatgttttcg gagccttatt acctgtagta
    47101 gattctaata aggaacaaga ccaagaatta ctagaagcta tttaccaaac agatacactt
    47161 gaagctgtac tgacactgat ccagaaccat tacggcattg atcctttttc tgctaaagaa
    47221 aaattagcag agctagttca aaggatcgaa gctgattatc cagatgcaat ccattacagc
    47281 tattaccctt ctgaaatcgt tcgagcgatt gaaaaaggat atttattaga gattcaagag
    47341 ccgactgtca tccgagatgc ctctgttctt gtggcactta actcagcgct cgaaccaaat
    47401 gggatgttga atttaccgac aggaatcatt cgtcgccatc cagattgcgt cattgtgatc
    47461 actacaaatc gaaattacca aggaaatcgc ccattaaatg aatctttaag agatcggatg
    47521 caacatgcgg aaaaaatgga tcttcctgcc ttagaagtca tggcagaacg tgcgatggcg
    47581 aaaactatga tccaaaaaca agaacttctc ttaaagatgg cagagattat ccgtttgttg
    47641 gatgaaactg caaaagctaa cgccatcaaa ggtgtagcag gaatgcgttc ttatttttat
    47701 tgggcaaata cctataagca aggacaagat ttactccagt cgatttatcc aaaagttttg
    47761 tataaattat ccactgattc agatgaattg gcaattcttg aacaagcttt gtctgacagt
    47821 ggattgctac tggaacttca ggacatattg agaaaagaaa aatggggtca tcctcaatct
    47881 gagcatacaa aaggaagaac cattagcgcc gaagaagcaa atgaacgaaa catcactgaa
    47941 aatgctgaag aaattctgcg cttagatgaa aagaaatcgt tgtctgctga agaaccaaat
    48001 gagggagaag agaaacaaga agaaacaata gaaaaaaacg agattcctcc tcaaatgcaa
    48061 aaagaacgca gtagcgatat cgaaggaaaa aatcaaaaag aacaacaaac aggagatgat
    48121 tctcaagaaa tgtctgcttc tgacatggaa gcacatgata aggaaatcaa gaagcaactg
    48181 aataaagaag cgcgacaaat catgaaggaa acgattcatg aaaaagaagg tctcatcgtt
    48241 caccgaccag ctttccaagg aaaaacgccg gaagcacgtg agctacacat gcaagtaatg
    48301 ccaatagtag aatcacttag ccggcaaatt ttagagttac tggataatga acaaacagag
    48361 acgtaccaaa aaggaaaata cgaaggtcag cggttcaatg caagcaaagt agcatatggt
    48421 gatttgagaa attttgacaa aaagaacccg ccacatgagc aaccttcttt agcggtagct
    48481 gtacggatcg atgaatctgg gtcaatgata cgggatgatc ggatcgaagc ggcaaaaaaa
    48541 gcagcgattg ccattgccga attttctaag aaagtcgata tcccattgtt gatttacgga
    48601 gatacagcag accgttcgtc aagagaaaag acttctttgt tttcttataa agaatttgaa
    48661 gacggatttc aatggttaaa tgaaaaatta gtcactatga aaccaaggca aaacaatcgt
    48721 gatggtgctg cacttcgttt agcagcagaa aaattaaaca gacaatccgc aacaaccaaa
    48781 ctgttgctta atattagtga cggtcagcca aaagcgttgc cggattatac aggcaaaaaa
    48841 gcaaaagaag atatccagtc tgttttgaaa gaatatgaac gtcaaggaat cctatttgtc
    48901 tccgcagcta ttggccaaga caaagaagaa atcaagtcga tctacggtga aagtcgtttt
    48961 gtagatatta ctgacttgaa tgaatttccc aagcaaatga ttcaactgat tgcccgctat
    49021 ctatgataaa ataaagctca acgaacgcac ttagcagatt cgttgagctt ttcgtttcta
    49081 gttaccggta acaaggatac ttctaaaata gaatcggtta taatcaagag ggaccgttaa
    49141 aggagtgaaa cgatatgata caagaacaaa aatggtggaa agaagcagta ggctatcaaa
    49201 tctatcctgc tagtttcaaa gatagcaatg gcgatggtca aggagacatc aatgggatcc
    49261 gtgaaaaact taattatttg aaagatcttg gtatcggctt tatctggatc aatccgatct
    49321 atcaatctcc atttgtcgat aatggttatg acgtaagtga ctatcaagat atagaagaga
    49381 gattcggaac gatggaagat tttgatttat tgctaaaaga agcacatgaa ttagggatca
    49441 aagtgattat ggatcttgtc atcaaccata gttcggatca gcatcaatgg tttgaagaat
    49501 cgaaaaaatc taaagaaagc ccgtatcgcg attattacat ttgggtcgac ggggtagatg
    49561 gcaaagaacc gaataattgg acatcgattt tcggcggttc tgcttgggag tattctcatg
    49621 aaactggaca gtattatctg catgtttttg caaaagaaca accagactta aattgggaaa
    49681 gcgagaaatt gaaagaagaa ctattcaata tgatccgatg gtggttggat aaagggatag
    49741 atggatttcg tttagatgcg atttctcatg taaaaaaaga tgaatattca gtaaaagcca
    49801 cagagaaccc tttctcaccc tttcaaaatg tctcaggaat cgaagaacat ttgaccgatt
    49861 tgaaacatgt ttttgaagag tacgatatca tgactgtcgg agaagcaagt ggtgtcactg
    49921 cagaagaagg accacaatgg gtagggaaag acggttattt cgatatgata ttcgaatttg
    49981 atcatatcca tatctggcag caagaaaaag aagggcaatt agatgtcctt aaattgaaac
    50041 ttgcattaag cgcttggcaa acttcattgg atggtatcgg atggaacgct ttatatatgg
    50101 aaaaccatga tgttcctcgt gccgtttcag tgtttggaga tactcgtccc gatttttggg
    50161 ctatgtctgc aaaagcaatc gctatgatgt atttcttttt gcaaggtacg ccatttattt
    50221 atcaaggaca ggaaatcgga atgacgaata tgccctttga atcgatcgat caagttgatg
    50281 cagtagattc gaaaagactt tataaaaggc tgcttgcgga aggtaagaca agagaagaag
    50341 cattggatat cattcgtgaa acaacgagag acaacagcag gacacccatg caatggactt
    50401 cggaacaata tgctggtttt tctacacatg aaccttggct gatcactaat ccaaatacca
    50461 aaacgatcaa tgtagaacaa caagaatatg aaccagagtc ggtcctgcaa ttctataaga
    50521 acatgatccg cattagacaa accaataaag gtctgatata cggttcttat aaagaatatc
    50581 ttcatgaaca tccacaactt tatgtctatg aaagatattt agaagatgaa caatatctta
    50641 ttatggtaaa cttaacagaa tcacttgctg actatgaact tccaaaagaa gcagatcagt
    50701 cctggacatt actcttgtca aattcctcta gcggagaatt tgaagctaaa ggcatactag
    50761 caccttatga agctcggtta tataaaacaa acaagtaaaa cttcctcgtt aaagttcatt
    50821 agttggactt tgcgaggaag tttttcagat tgctctgtca attactcatt tcaataattc
    50881 gctctaggac caccgatata tttaggacga gagcgaaggg ctgtcacgtg agcttgtcca
    50941 agggttttca aacgaggtct gacaggtact tggacatgtt tgatatgcat accgattgat
    51001 gtgtcaccga tatcgatacc ggcttgtcca actacgtgtt ccacttcgac aggatcattg
    51061 aactggtcaa aagcagcaac agaacaagca cctccagcat gtagtgcagg aacaacagag
    51121 acaatttcga aattttttgc ttctgccact gttcgttcta ctactaatgc tcggttcaaa
    51181 tgttcacaac cttggacagc tagcgcaatt tctttaggat ccaacaactc ttttaacgta
    51241 cggatgatcc attgaccaac ttccgcactg gaattttttc caatatgtcc tccaacgact
    51301 tcgcttgtgc tgcatcctaa aacaaaaaga tcaccttttt tcaagtgtgc ctcagccaat
    51361 atgtcattaa ctatttcagt caattggtct tttaattctt tttctgacaa cttcattttc
    51421 taaaaccttc tttttcaatt aatattgctt ccgtgcttga atacgttgaa gacttgcagt
    51481 tataggaaag gcaagtccca ttccaatcag attttggaag atgtttcctg gtatagaagc
    51541 cactccagcc ggccaatcat ataaaatcca accggcgata ccatagccga tgatcataac
    51601 taagcttcct aataaaagcc ctaatcccca catacgagta gaagatttgc tgaaagaacc
    51661 agcaacatat ccttgtaacc cgtggatcac taatgaaaaa actgcccact gcggatatcc
    51721 tgaaatcaaa tcgatcaata gaccactgga agcaccaaca actaatccac ccgttggacc
    51781 ataaagaaaa gcagcggtat agatcccggc ctcgcataat gtcacaaatc cattcgtagc
    51841 cggtacaggg atcaaaattg ttaatgaaag ggcaactgtc aacgcaatca gcatagcata
    51901 gatcgttaat tgcttagttt tcatttctca ttcctccttt ttgaataccg ccagaccaga
    51961 cacttccggt atgatcgttc atcagcacgc cattagaaat acaatgggca acaaattttt
    52021 tactttctgc tactgctttg gtaaaatctg cccctaacac aagattcgct gttattgcag
    52081 aagaaaaggc acaaccggcc ccgtttactg ttgtagtgtc gattttttta tgagaaaata
    52141 cctcataatg atcaattgaa tagagaagat ccacagcttc atctccagcc atccttgtgc
    52201 cgcctttgat cacaatgttt tttgttcctg aagcagtgag ttctttagcg agatggatca
    52261 tttctgttaa cgaagaggga agagcttgac cagaaagtaa agcagcttct gccaaattag
    52321 gcgtaatcat cgtaacaagt ggaaaaagtt cttcgatgag ctgcgaaata tacaattgat
    52381 tgatcacagc ttcagtttct ttgaatgcca aaacagggtc taaaatgatt gggatatttt
    52441 gttttgtttt tatgaagtct cgaacaacca tgattgcttc acttgaattc aataacccaa
    52501 ttttgatacc atctaatgaa gtcattttat caatcgaaac taactgtcgt ttaagcaatt
    52561 ccaaagatat agattccagc ataaatcgct cattaatatc tagtgcccct atagaagtca
    52621 aggcgcttag tccgaataga ccaaaattct caaaagtttt taaatctgtt tggattcctc
    52681 caccagcaaa tggatcagaa ccgccaattg tcaagattct tttcataaca ttttcctcca
    52741 tgaataagag tataacgaaa ttttcaaaaa agaaaaacag ccaattggca tatttttatc
    52801 tcatccaatt taaaaaaaca aaaagaaaag aaagaccaaa atggatcttt ctttccttaa
    52861 atagttagga cttttgtgat tttaattctt tctcgatttg aagccgttgc actttcattg
    52921 tagctgttcg cgggattgca tcaaaatcac ggatgatagg gacgttcaag cgaggaagtt
    52981 catggacttg ctcccaccat cgattccaat ccatttcttt atcaggagct aaagcaataa
    53041 tcggttgtgg agaatgattt ttatccctta caatcactac ttctgccaaa aaatctaacg
    53101 aatctaataa aaagtcttca atagctaatg tactattgat cgtttcgatc aaatccactt
    53161 gccgatcttt caaaaatagg tggccatgct tatccataaa accgtaatct ccgctatccc
    53221 accaatcgcc ataaacattt tcctggaacc gtgcatcttc cttgtaataa gtcaaagccc
    53281 gtcctttaga cagtaattgt atatgaccat ctgtgttttc aggtaggaca ttcccaactg
    53341 aatctgtaat ccgagcgctt gtcatgtctt ccagtccaac gcccatatct cttgcatcac
    53401 ttgttttcaa actctctagt gtatgagcct taagaatcat tggtccgcat tcactttggc
    53461 catatacttg aagaaatacc gcatcctgtg ccatcgacgt tcgcaaaaat gtcttcattg
    53521 tctggttgtt tatagcatca aaagtggagt gatagtatcg gatacccgaa aaagcttctg
    53581 gatgctcctt agcagtaaaa cgccactgaa caaagttatt cggatgggtc tctagtgcaa
    53641 ttggccggtg agcttccaac atatgaacaa cttttgaact ttgtgcgttt gctaaaggca
    53701 tcataggaaa tcccatggac atcaaagaag agataccaat attgaatctg gaatgcactg
    53761 gagagatgtg aaaagcgatt aatttctttt ccgaaatttt tgtgaaaact gttttttgcc
    53821 atttcgtccg ccatcccata gaatgagcag aatgacaaat aagtttaggg ataccagttg
    53881 taccagaagt atgggtcata taagaaattt catttttgtc taaaaatgat tgagaaacag
    53941 acaaagcagg ttgttccatt aaataaagaa tagg
//
LOCUS       DACDIP010000012        51744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDIP010000012 DACDIP010000000
VERSION     DACDIP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.012"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IV257_000881"
     CDS             complement(61..2424)
                     /locus_tag="IV257_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP8814028.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="IV257_000882"
     CDS             2872..3093
                     /locus_tag="IV257_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8814029.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IV257_000883"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IV257_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8814030.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IV257_000884"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IV257_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8814031.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="IV257_000885"
     CDS             complement(5894..6205)
                     /locus_tag="IV257_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8814032.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="IV257_000886"
     CDS             complement(6202..6495)
                     /locus_tag="IV257_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8814033.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IV257_000887"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IV257_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8814034.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IV257_000888"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IV257_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8814035.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="IV257_000889"
     CDS             complement(8333..12685)
                     /locus_tag="IV257_000889"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8814036.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="IV257_000890"
     CDS             complement(12897..14606)
                     /locus_tag="IV257_000890"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8814037.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IV257_000891"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IV257_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8814038.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="IV257_000892"
     CDS             complement(16103..16903)
                     /locus_tag="IV257_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8814039.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="IV257_000893"
     CDS             complement(16900..17712)
                     /locus_tag="IV257_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8814040.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IV257_000894"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IV257_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8814041.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="IV257_000895"
     CDS             complement(18467..19189)
                     /locus_tag="IV257_000895"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8814042.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="IV257_000896"
     CDS             complement(19325..20206)
                     /locus_tag="IV257_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8814043.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IV257_000897"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IV257_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8814044.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="IV257_000898"
     CDS             21446..21925
                     /locus_tag="IV257_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8814045.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="IV257_000899"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="IV257_000899"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8814046.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /locus_tag="IV257_000900"
     CDS             23226..24011
                     /locus_tag="IV257_000900"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8814047.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="IV257_000901"
     CDS             complement(24357..26048)
                     /locus_tag="IV257_000901"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8814048.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IV257_000902"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IV257_000902"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8814049.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IV257_000903"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IV257_000903"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8814050.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IV257_000904"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IV257_000904"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8814051.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            30332..31033
                     /locus_tag="IV257_000905"
     CDS             30332..31033
                     /locus_tag="IV257_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8814052.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IV257_000906"
     CDS             complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IV257_000906"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8814053.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IV257_000907"
     CDS             complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IV257_000907"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8814054.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IV257_000908"
     CDS             complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IV257_000908"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8814055.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IV257_000909"
     CDS             complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IV257_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8814056.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(34528..35508)
                     /locus_tag="IV257_000910"
     CDS             complement(34528..35508)
                     /locus_tag="IV257_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8814057.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IV257_000911"
     CDS             complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IV257_000911"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8814058.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IV257_000912"
     CDS             complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IV257_000912"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8814059.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            38266..38433
                     /locus_tag="IV257_000913"
     CDS             38266..38433
                     /locus_tag="IV257_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8814060.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(38495..40306)
                     /locus_tag="IV257_000914"
     CDS             complement(38495..40306)
                     /locus_tag="IV257_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8814061.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(40303..40668)
                     /locus_tag="IV257_000915"
     CDS             complement(40303..40668)
                     /locus_tag="IV257_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8814062.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(40831..41226)
                     /locus_tag="IV257_000916"
     CDS             complement(40831..41226)
                     /locus_tag="IV257_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8814063.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(41244..42206)
                     /locus_tag="IV257_000917"
     CDS             complement(41244..42206)
                     /locus_tag="IV257_000917"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8814064.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNIYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(42206..42418)
                     /locus_tag="IV257_000918"
     CDS             complement(42206..42418)
                     /locus_tag="IV257_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8814065.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(42439..43137)
                     /locus_tag="IV257_000919"
     CDS             complement(42439..43137)
                     /locus_tag="IV257_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8814066.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(43157..43699)
                     /locus_tag="IV257_000920"
     CDS             complement(43157..43699)
                     /locus_tag="IV257_000920"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8814067.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(43831..44835)
                     /locus_tag="IV257_000921"
     CDS             complement(43831..44835)
                     /locus_tag="IV257_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8814068.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(44832..45821)
                     /locus_tag="IV257_000922"
     CDS             complement(44832..45821)
                     /locus_tag="IV257_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8814069.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(45818..46624)
                     /locus_tag="IV257_000923"
     CDS             complement(45818..46624)
                     /locus_tag="IV257_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814070.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            46790..47746
                     /locus_tag="IV257_000924"
     CDS             46790..47746
                     /locus_tag="IV257_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8814071.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(47823..48341)
                     /locus_tag="IV257_000925"
     CDS             complement(47823..48341)
                     /locus_tag="IV257_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8814072.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IV257_000926"
     CDS             complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IV257_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8814073.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            48806..49252
                     /locus_tag="IV257_000927"
     CDS             48806..49252
                     /locus_tag="IV257_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8814074.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IV257_000928"
     CDS             complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IV257_000928"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8814075.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        14563 a        11043 c         9071 g        17067 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
    30241 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
    30301 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
    30361 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
    30421 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
    30481 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
    30541 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
    30601 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
    30661 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
    30721 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
    30781 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
    30841 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
    30901 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
    30961 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
    31021 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca gggtcactgc
    31081 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg ggcaagactc
    31141 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgata
    31201 gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata ttcctcgaca
    31261 ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata agctgtaaaa
    31321 aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt gtgtacctgt
    31381 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
    31441 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
    31501 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
    31561 gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc agataaaacc
    31621 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
    31681 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
    31741 tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
    31801 gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca agctcgaagt
    31861 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
    31921 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
    31981 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa atggtaatta
    32041 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
    32101 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
    32161 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
    32221 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
    32281 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
    32341 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
    32401 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
    32461 tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact gtttatttga
    32521 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
    32581 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
    32641 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
    32701 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
    32761 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
    32821 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
    32881 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca actaaaagca
    32941 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
    33001 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
    33061 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
    33121 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaaaccta
    33181 acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
    33241 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
    33301 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
    33361 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
    33421 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
    33481 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
    33541 aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct tgaaaaaagc
    33601 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
    33661 aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc ttgtttctct
    33721 gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg aatatcccat
    33781 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata gaagaatttg
    33841 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
    33901 tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc tttccagcgg
    33961 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aattttatcc
    34021 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
    34081 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
    34141 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
    34201 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
    34261 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
    34321 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
    34381 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca atgatacatg
    34441 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
    34501 ttcatttccc cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt
    34561 ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc
    34621 cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagag agggcgttac
    34681 atatttcgct acactgacat cattgaaacc catcacagaa atatcttctg gtaccactaa
    34741 acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag
    34801 cgcatcagga acatgcggat tcgtttttaa atactgcttc actgcttgat atcctgactc
    34861 gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg
    34921 ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt
    34981 tccagcaagc atagcgatat gttgcgcttt atgggagaca aaatgttcaa tgactttttg
    35041 aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc
    35101 taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt
    35161 tcctagtgcg atcgttccat ctgtcggaag atcactaatg gaatcccacg tttctttcaa
    35221 taaagtaacg tttgcttctt ctgccttttt ttcgattcct agacgaatag ataaataata
    35281 aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt
    35341 atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt
    35401 ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt
    35461 agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc
    35521 cttttttacg tttataaaat tgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct
    35581 cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt
    35641 acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc
    35701 cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct
    35761 actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca
    35821 atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt
    35881 tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt
    35941 cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat
    36001 actgtttcat ccgaatatgc tttccacttt tccaatactg agttagctgc ttcaatcaac
    36061 aattttttct ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg
    36121 atgacttgcg gataatctgc tagtttgata agtttttcta tcggtgcttt tgccatagga
    36181 aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaatcggc
    36241 aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta
    36301 gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt
    36361 tcattgtaat aagcataagg agaatattgg aatccccagc tttcaccgtc tagattcatt
    36421 cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt
    36481 tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt
    36541 tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc
    36601 gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc
    36661 ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca
    36721 aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa
    36781 atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc
    36841 tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca
    36901 tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat
    36961 ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct
    37021 cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga
    37081 taattgcttt gatatctgtg tattcaggtt tccaacccag aacctctctc gctttttcac
    37141 ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt
    37201 cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc
    37261 tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat
    37321 gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat
    37381 cataatcgtc tccataaaca gccaatgctt ttctttgacc aagagctact tgtaaaatga
    37441 ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc
    37501 cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact
    37561 tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg
    37621 tatcttctgt gattggagac tcttttggtt ctccgtaagt tgctgcagta gaagagaaca
    37681 cgattttatt cacattgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt
    37741 tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa
    37801 aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc
    37861 ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca
    37921 aattatctac cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac
    37981 cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta
    38041 aaattgtgtt tgttgattat atataattta aaaaaacgaa gtttactttt atggtgaaaa
    38101 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
    38161 attttcaagg attttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct
    38221 tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta
    38281 tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggtcgcttct
    38341 gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt
    38401 aaaaaagcaa tgcgcaaacg cgtttctcgt taataattat ttaaaaacac gcatgaagac
    38461 ttgtgaaaga acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
    38521 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
    38581 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
    38641 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
    38701 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
    38761 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
    38821 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
    38881 caaatggcgg ttcgaacaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
    38941 tccgaatgac tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
    39001 gtatttcttt gtattccttt gcagaaaaaa cgtgccattc aatagcctgc cggattggat
    39061 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
    39121 ccgcttcttt tatctgccct tcaataaatg ctcggctagc ttctgcaaag gactcatcct
    39181 gataggtaag aggaatttct aaatacgacc aatcccatct ataagggatt agtgctggat
    39241 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
    39301 gaatcggcaa atgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
    39361 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
    39421 tttcttttgt tagtaaccgt ggccatgcca ttgatccgcc ttgcttttgg tttcttcctc
    39481 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
    39541 gatgatagac aagttttcct ttttcgttct cgaagcgccc ttctcttgat actgtaaaca
    39601 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
    39661 gtatcttcga aagatcgaca tctgcaggat tagaaggaag ctgatacagt aaatcatttt
    39721 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
    39781 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
    39841 cttttctttt gctgactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
    39901 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
    39961 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
    40021 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
    40081 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
    40141 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
    40201 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
    40261 ctatacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
    40321 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
    40381 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
    40441 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
    40501 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
    40561 taactgcgct ttttcttttg aaattcctcg tggccagaac ctatccacga gtattctata
    40621 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
    40681 ctttctgcct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
    40741 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
    40801 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
    40861 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
    40921 atttgctgta cggatgctca agcttttctt ttgatcgtaa atatagacag gctggtcttt
    40981 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
    41041 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
    41101 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgc ttggccatta ctcgtagata
    41161 gacttcccaa aagatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
    41221 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
    41281 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
    41341 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
    41401 actcctccac ctaacacaat acttgacgga ttcaatgtat tccccaagtt ggcgcaagct
    41461 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
    41521 gccatttcaa agatatcttt acttgttact tcttcaccgt tgtctaacat tgctttcagt
    41581 tttgaatctc ctgcgtattc ttctgccaag taacgaccta gacgtaccac accagtagcg
    41641 ctagacacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
    41701 acagtgatat ggccgatctc tccgcctgct ccgctcacgc cgtgaatcaa atttccgcca
    41761 gcaacgatac caccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
    41821 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcatcatt atcgatagcg
    41881 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
    41941 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
    42001 ccgattccga taaagtcttc ggctttcata ttatatatat tcaaacgatg gttgattgat
    42061 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
    42121 ttttgttgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
    42181 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
    42241 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
    42301 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
    42361 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
    42421 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
    42481 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
    42541 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
    42601 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
    42661 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
    42721 taagataaac aacgcaccga acaaaccgaa tatcgaagta ctcgcaccac cagacagaac
    42781 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
    42841 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
    42901 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
    42961 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
    43021 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
    43081 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
    43141 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
    43201 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
    43261 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
    43321 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
    43381 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
    43441 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
    43501 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
    43561 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
    43621 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
    43681 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
    43741 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
    43801 aaatgattgg ccttcctttt ttaataaatt tcaactgttc agcgcgtgtt tttgtctgat
    43861 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
    43921 ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
    43981 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
    44041 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
    44101 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
    44161 ttgcgcgcgt tcttgaccaa atgatagcaa tccaatcctc tgtgcataaa gtcctcctag
    44221 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
    44281 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
    44341 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
    44401 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
    44461 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
    44521 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
    44581 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
    44641 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
    44701 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
    44761 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
    44821 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
    44881 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
    44941 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
    45001 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
    45061 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
    45121 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
    45181 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
    45241 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
    45301 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
    45361 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
    45421 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
    45481 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
    45541 cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa tgcaccgctc
    45601 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
    45661 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
    45721 tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
    45781 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
    45841 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
    45901 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
    45961 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
    46021 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
    46081 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
    46141 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
    46201 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
    46261 actcgattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
    46321 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
    46381 ataatgtaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
    46441 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
    46501 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
    46561 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
    46621 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
    46681 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
    46741 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
    46801 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
    46861 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
    46921 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
    46981 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
    47041 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
    47101 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
    47161 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
    47221 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
    47281 agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa aatccatcaa
    47341 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
    47401 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
    47461 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
    47521 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
    47581 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt
    47641 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
    47701 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
    47761 agaatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta gacgcataga
    47821 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
    47881 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
    47941 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
    48001 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
    48061 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
    48121 ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg ataaggaagt
    48181 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
    48241 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
    48301 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
    48361 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
    48421 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    48481 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    48541 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    48601 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    48661 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    48721 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    48781 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    48841 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    48901 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    48961 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    49021 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    49081 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    49141 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    49201 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    49261 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    49321 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    49381 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    49441 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    49501 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    49561 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    49621 tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    49681 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt ccggaagatg
    49741 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    49801 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    49861 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    49921 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    49981 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    50041 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    50101 tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt tacgtcctaa
    50161 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    50221 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    50281 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    50341 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    50401 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    50461 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    50521 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    50581 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    50641 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    50701 gtctagtttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    50761 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    50821 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    50881 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    50941 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    51001 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    51061 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    51121 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    51181 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    51241 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    51301 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    51361 tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat agcggacgaa
    51421 tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca
    51481 gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta ccttattctc
    51541 ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaacagcaaa aaaagacacg
    51601 acctaaagat cgtgtctaat tatgactgta tcagaaaata tgataccaaa cggatctatt
    51661 cattaattaa ttgacatagt ttatctgttg ctcttttatg actggataac tgaccaattc
    51721 atgacgatcc aagtcttctt tatt
//
LOCUS       DACDIP010000013        48470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDIP010000013 DACDIP010000000
VERSION     DACDIP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.013"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2546)
                     /locus_tag="IV257_000929"
     CDS             complement(<1..2546)
                     /locus_tag="IV257_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8814076.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLN"
     gene            complement(2559..3776)
                     /locus_tag="IV257_000930"
     CDS             complement(2559..3776)
                     /locus_tag="IV257_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8814077.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(3766..5361)
                     /locus_tag="IV257_000931"
     CDS             complement(3766..5361)
                     /locus_tag="IV257_000931"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8814078.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(5871..6653)
                     /locus_tag="IV257_000932"
     CDS             complement(5871..6653)
                     /locus_tag="IV257_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814079.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(6718..7554)
                     /locus_tag="IV257_000933"
     CDS             complement(6718..7554)
                     /locus_tag="IV257_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8814080.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(7572..8981)
                     /locus_tag="IV257_000934"
     CDS             complement(7572..8981)
                     /locus_tag="IV257_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8814081.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(8995..10818)
                     /locus_tag="IV257_000935"
     CDS             complement(8995..10818)
                     /locus_tag="IV257_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8814082.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(11661..11987)
                     /locus_tag="IV257_000936"
     CDS             complement(11661..11987)
                     /locus_tag="IV257_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814083.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(12033..12395)
                     /locus_tag="IV257_000937"
     CDS             complement(12033..12395)
                     /locus_tag="IV257_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8814084.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(12432..12647)
                     /locus_tag="IV257_000938"
     CDS             complement(12432..12647)
                     /locus_tag="IV257_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8814085.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            12774..13109
                     /locus_tag="IV257_000939"
     CDS             12774..13109
                     /locus_tag="IV257_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8814086.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(13185..15182)
                     /locus_tag="IV257_000940"
     CDS             complement(13185..15182)
                     /locus_tag="IV257_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814087.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(15280..15555)
                     /locus_tag="IV257_000941"
     CDS             complement(15280..15555)
                     /locus_tag="IV257_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8814088.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            15787..16521
                     /locus_tag="IV257_000942"
     CDS             15787..16521
                     /locus_tag="IV257_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8814089.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            16560..17702
                     /locus_tag="IV257_000943"
     CDS             16560..17702
                     /locus_tag="IV257_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8814090.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(17797..19188)
                     /gene="sufB"
                     /locus_tag="IV257_000944"
     CDS             complement(17797..19188)
                     /gene="sufB"
                     /locus_tag="IV257_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8814091.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(19193..19663)
                     /locus_tag="IV257_000945"
     CDS             complement(19193..19663)
                     /locus_tag="IV257_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8814092.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(19650..20885)
                     /locus_tag="IV257_000946"
     CDS             complement(19650..20885)
                     /locus_tag="IV257_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8814093.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(20882..22168)
                     /gene="sufD"
                     /locus_tag="IV257_000947"
     CDS             complement(20882..22168)
                     /gene="sufD"
                     /locus_tag="IV257_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8814094.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(22181..22954)
                     /gene="sufC"
                     /locus_tag="IV257_000948"
     CDS             complement(22181..22954)
                     /gene="sufC"
                     /locus_tag="IV257_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8814095.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(23151..23999)
                     /locus_tag="IV257_000949"
     CDS             complement(23151..23999)
                     /locus_tag="IV257_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8814096.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(24023..24709)
                     /locus_tag="IV257_000950"
     CDS             complement(24023..24709)
                     /locus_tag="IV257_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814097.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(24702..25736)
                     /locus_tag="IV257_000951"
     CDS             complement(24702..25736)
                     /locus_tag="IV257_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8814098.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(26131..26469)
                     /locus_tag="IV257_000952"
     CDS             complement(26131..26469)
                     /locus_tag="IV257_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8814099.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(26471..26827)
                     /locus_tag="IV257_000953"
     CDS             complement(26471..26827)
                     /locus_tag="IV257_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8814100.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(26833..28020)
                     /locus_tag="IV257_000954"
     CDS             complement(26833..28020)
                     /locus_tag="IV257_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8814101.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(28211..29056)
                     /locus_tag="IV257_000955"
     CDS             complement(28211..29056)
                     /locus_tag="IV257_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8814102.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            29290..30255
                     /locus_tag="IV257_000956"
     CDS             29290..30255
                     /locus_tag="IV257_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8814103.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            30632..31675
                     /locus_tag="IV257_000957"
     CDS             30632..31675
                     /locus_tag="IV257_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8814104.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(31737..32201)
                     /locus_tag="IV257_000958"
     CDS             complement(31737..32201)
                     /locus_tag="IV257_000958"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8814105.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            32447..33394
                     /locus_tag="IV257_000959"
     CDS             32447..33394
                     /locus_tag="IV257_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8814106.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(33605..35677)
                     /locus_tag="IV257_000960"
     CDS             complement(33605..35677)
                     /locus_tag="IV257_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8814107.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(35610..35987)
                     /locus_tag="IV257_000961"
     CDS             complement(35610..35987)
                     /locus_tag="IV257_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8814108.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(36116..36676)
                     /locus_tag="IV257_000962"
     CDS             complement(36116..36676)
                     /locus_tag="IV257_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8814109.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            36826..38118
                     /locus_tag="IV257_000963"
     CDS             36826..38118
                     /locus_tag="IV257_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8814110.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(38178..39296)
                     /locus_tag="IV257_000964"
     CDS             complement(38178..39296)
                     /locus_tag="IV257_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8814111.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(39310..40731)
                     /gene="arcD"
                     /locus_tag="IV257_000965"
     CDS             complement(39310..40731)
                     /gene="arcD"
                     /locus_tag="IV257_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8814112.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(40977..42098)
                     /gene="mnmA"
                     /locus_tag="IV257_000966"
     CDS             complement(40977..42098)
                     /gene="mnmA"
                     /locus_tag="IV257_000966"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8814113.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            42502..43101
                     /locus_tag="IV257_000967"
     CDS             42502..43101
                     /locus_tag="IV257_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8814114.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(43239..44525)
                     /gene="efmA"
                     /locus_tag="IV257_000968"
     CDS             complement(43239..44525)
                     /gene="efmA"
                     /locus_tag="IV257_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8814115.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(44796..45140)
                     /locus_tag="IV257_000969"
     CDS             complement(44796..45140)
                     /locus_tag="IV257_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8814116.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(45277..46413)
                     /locus_tag="IV257_000970"
     CDS             complement(45277..46413)
                     /locus_tag="IV257_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8814117.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(46602..47576)
                     /locus_tag="IV257_000971"
     CDS             complement(46602..47576)
                     /locus_tag="IV257_000971"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8814118.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(47656..48219)
                     /locus_tag="IV257_000972"
     CDS             complement(47656..48219)
                     /locus_tag="IV257_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814119.1"
                     /translation="MEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVL
                     LAYYQDYRKGWMLPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKK
                     NGNHERARATLLGSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGER
                     YYKVHDNWGDYHKVIPASWSNGTVSLP"
BASE COUNT        13834 a        10205 c         8437 g        15994 t
ORIGIN      
        1 ttgttcaaag ttggagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
       61 tcataagcac gattcaattc ttgaaatgct ttgacgtact cgatacggat ttcaagtggc
      121 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgtttg
      181 aatctttttt tggattcctg ataacttgga taaatcaaac ctgattcttc ttttgctttt
      241 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tcgctggctt acgaaacgtc
      301 acaatcaatc cttttgtctt atcaggatac gtacgattcg tacgtgaaaa agcttggatc
      361 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg ggcgtcaaaa
      421 cctgttaata gacgatccac tacaatcgct aaatcgattt gtttcccaaa ttgtttgaat
      481 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
      541 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
      601 tgttgggcac ttgaatctcg gctattttct tctaatgaat aggtaatagc gattcgtgga
      661 aaatcaggat cctccatcgt tcgtccttca cgaatgagct gattgggaaa ctcttttgtt
      721 aaccaatcag gctctttcgt catctctttg atggcccgat aatacttttt ggccatgtca
      781 atggaactcg tggttaagat tgcagatttc gttggcctgc catttcgaaa atcaaatttt
      841 gtataggcat tatctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
      901 tcaaaaacag ctgggtccaa ataactttct tttttgacag gttccatttg atcaatcata
      961 cgattgatct gttctgatga atactctgca tatgtttcta cttcttttaa tttccgatag
     1021 attttattct ctagtgaagt cggctccacg gtgttttcat gctctacttg aaatcctaag
     1081 acagagccat cttctagcgc attcttaatg gtataggtat gcaagacctc tccatactga
     1141 tcatgggttg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg aaaaatcggt
     1201 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     1261 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg gcccattaat
     1321 ttttggaagc gattggttcc ttttttctct tcttgtttct ttgcatattt caacgccgca
     1381 tctagttttt gacgggttgt aatgattaca acatttgcat tggcatctgc caacagggtt
     1441 tcacttaatt ctttggcact tcccgttcca acgatcaagc tattcgcttt ggcgttcccg
     1501 gaagaaatcc cagtattaaa ttccgaagca aattttgtaa actctgtggt ggtttgatta
     1561 tctaaatctt tgcggtccac taacatgatc gttcgatcga tccccgattt tcgtgctaaa
     1621 agcttagtag atacaaaact agtcaacgtc tttcctgaac cagtcgcatg ccacacatac
     1681 cctgattgat gtttatttgc ggcaataaac aaggcttgaa tcgcatgtac ttgataagga
     1741 tgtaacacca tgagggtttt attgtcttga tcttcactga cgattgtata atccgcaatc
     1801 aaacgatgag catccgggat atttaacact tgtttacaaa actcatataa gttttctact
     1861 ttttcattgt ctttcgttcg ccaactaaat aaaaacttag ggtgcaagtc tttcggtaag
     1921 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     1981 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaatgcctga
     2041 taaacaccat cttttgcagt tacttgtttc agctcgattt ggacaatcgg taatccattg
     2101 attaataagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     2161 atttgatgga ccacttcata agtagagatt cctccaccaa tgtcttgatt ggaataaagg
     2221 ataagtgaga cagaacccaa ttgcgaatct tcccgttcaa tcgtgatccg agccatccca
     2281 ttttcacctt ttaaccattt cgcagcatca aatggggttt tcgttcgcag cagtaattcc
     2341 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     2401 tttgagataa ttttttggcg taaattgacc caaaggtctt cttctgattt tagatccgga
     2461 cgatacgtcc actgattatg cccttctcca agtacacgaa tcaactgttc ttcgacttct
     2521 gcttcctcac ggtgactaat tttcattctt cttccctcct agacaaacat cttttgtaag
     2581 aagctttttt tcgtttcttt tagtaaatct aacttacgtt gatgaagagc gatcgtatca
     2641 tctagctgtt tgaagaaagt accgattttt tgttgttcat cctttacagg aaccttaaca
     2701 ctgatattct caatatcaga actgttataa gaaggcaatg cccctacttg ggagtatctt
     2761 ggcaaattta tagtttcaaa caatatttta ccaaaataga tatcccaagt attattaaat
     2821 aaataagcca tggtattgtt atctattaag aaagggcttg tgaccaaacg ttttctatta
     2881 agcattattg ctgcgccaac tttagcaaat ataatcgctg gcacatcttt tattggcttc
     2941 cattttttac gctcaatttg ctcattagat acataatgat ttgcatttct catttcatac
     3001 tcattaccaa tgttattcat gtcggaaact ttaaaaaatg gagtaccttc agaaccacct
     3061 tgctcggcat caggaaatcc aattcccgat tgagttttac ctaattctcc caacttacgc
     3121 tcttcccaat cttccgtaaa accaggaaac cggatttctg gcacttttgc tccgttttca
     3181 gggaacattt tttgtaagaa tccttttttc gtttccttca gcaaatctaa cttacgctga
     3241 tgaagagcga tcgtgtcgtc aagttgtttg aagaaggtgc cgattttttg ttgttcttcc
     3301 tcactaggaa acattgctac tatattttta aactttttta gagacagaac atgttgacct
     3361 gttccctctg cttgaataga taattgtcgt tggattccat tgttaatacg tcttaagaag
     3421 aagtcggaat cagcagattt taaagtaaat acaggataaa aatagcttat aatccctcta
     3481 tcaataatgt cattacggtt aaacacaaac acatcattat cacttcgact acggaacgtg
     3541 aaatatcctc taggtagtac aaagtaacca atattatttt ctgtagttac ttgccgattt
     3601 gcaaaatagt cttcttgcaa aactatccct ttttgttgag atgaagttag cataggaaat
     3661 tgattttgta cacttgtctt ttcaacataa tctacaacga agtcccccaa cttcctctgt
     3721 tcccaatcat ctgtaaatcc tgggaaacgg atttctggtt gtgtatcatt gctcatgttt
     3781 gaacacctcc agcgcacctt gtaaccacgc ttcattttca ggtgttgttt gcagggaaga
     3841 gatagcttct aaaagttttt tttctagcac ttgctgttct tgtcgtatct tcttgatgtc
     3901 atttccgaca tgaaccatat caattggttc ttcttcttcg aaagtatcta catagcgtgg
     3961 gatatttaag ttatagtcat tttctttgat ttcatcaaag gttgccaaat gagcgtattt
     4021 ctcgacatct tttctttcag cgtaggtttc gaggattttt tgaatgtttt cttccgatag
     4081 tttattttga tttttccctt taacaaattc acgactagca tcaatgaata atacatctcg
     4141 agtttgacga ttcttcttca acacaatcac cgtcgttgga atcgatgtcc caaagaacaa
     4201 gttcgcaggc ataccgatca cagcataaat actgccatct tctagtaatt tttgacggat
     4261 gaccccttct gcagcgccac ggaataaaac tccgtgtggt aagacgatgg ccatggtacc
     4321 tgtttccttc aaatgataga acccatgaag aagaaacgca aaatctgctt tggattttgg
     4381 cgctaatttc ccataacgat taaaccgaga atcatctaag aaagtcgtat ctgctgacca
     4441 attagccgaa taaggtgggt tcattactac tgcatcaaat gtgtaaggtt catctgttgg
     4501 ccagtcctta ttcaacgtat ccccattgcg gagattcatt tcttcggcgt cgacaccatg
     4561 taaaatcaag ttcatttttg ctagattgta tgtcgttgta tttagttctt gcccatgata
     4621 tttaacgtta tctgggtgtg ttaagtagtt acgtacgttt aacatcaatg aacctgaacc
     4681 catcgttgga tcaaaaacac taaagaatcg tcgttccttt tgatcgagcg ttacgatttg
     4741 cgccatcata tcagaaacca tatgaggtgt gtaaaattct ccggctttct tccctgcttc
     4801 tgaagcaaat tgactgatca gaaactcata cgcatccccg atcacgtccc catcgtgttc
     4861 taaaacatct acatcattca atttcttgat gacttcggta atcgtcacat ttcgttgttg
     4921 ctcatctgtt cctagttttt ttgattggag atccacatca tcaaataacc cattaaattg
     4981 attgtaggta gatgccaatt gcacaaaggc tttgtttaga tcgttcaatt ggaacgttgc
     5041 ttgttttgct tgatccgcta agacgttgaa aagatactct ggtgcaattg catatcccaa
     5101 aatatcaacg attgtggcaa tcagatcttc ttttgattct tcatctgaaa gtaattcacg
     5161 atacaacatt gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag
     5221 aaccacttgt tccaacaatc gatcggataa atatttatag aaaatcagtc ccaataaata
     5281 atttttatat tctgacgcat ccattttact acggagatta tctgccgcac taaataattt
     5341 ttgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata
     5401 ccctcttatt ttagcatacc cccttttata aaaagcaaaa aaaaaaagaa agaaacttat
     5461 tcttgctaat aaactacgcg aaaggagtct aacgttgttt gatataactt agactgatcg
     5521 ttaacttatt ttttatattt ctacatagtt atctttagaa ccatttcaga tacatatgaa
     5581 acaattacat atgatttttt cataaaaaaa cagtactgtc tagtagtata aaatatacaa
     5641 atttcttaaa aattttttca ctaaaatcac tacctgaaac agtctaaaac acttatatat
     5701 caacgtttaa tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc
     5761 aaatccgaaa aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg
     5821 gtcatataac aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata
     5881 acttttttaa atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa
     5941 tcttttttcc cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact
     6001 gcattactga tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa
     6061 atctctgtat cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt
     6121 ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta
     6181 tctacttctg tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta
     6241 actgcttctg tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt
     6301 ccatctttag tgtgcatagt taaaattccc acgcttttac taataaattc actataacca
     6361 gttctttcaa tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca
     6421 tgtaaaagca ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt
     6481 cctatacgga atacagcttt actgtctgat atctctatac cagcatcctt attattaatc
     6541 tgccaatctc caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact
     6601 tcactggtag aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca
     6661 ggagccttag cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca
     6721 gtacaccctt gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc
     6781 catctcttct tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg
     6841 ctgcttcact gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat
     6901 tcgggcaata aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc
     6961 aacctttttt tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac
     7021 cgagagcatg gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc
     7081 aatgtttcca gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat
     7141 agcatagcag ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg
     7201 attttgaaaa acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa
     7261 ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat
     7321 tcgcttgacg caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt
     7381 ttttagagca tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac
     7441 caaatcgcct ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc
     7501 ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt
     7561 caccgttctc tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt
     7621 tcttttttta ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa
     7681 ccataacgct tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa
     7741 taaccaaaaa catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct
     7801 agataatcaa ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc
     7861 ccatagccat tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat
     7921 gttaatcgta gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga
     7981 ttttcagctg cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta
     8041 gcggaaacat ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta
     8101 atcgcaataa aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta
     8161 ggatataatc cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact
     8221 gctacatcta agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct
     8281 tctggactgg aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct
     8341 ttaagattca actgatgata caagtgaatt actctagctt gagctaataa aacatggtga
     8401 ttaatttgaa agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct
     8461 ccaggatgaa ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca
     8521 ccatactcag taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt
     8581 aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc
     8641 ggttcgattc cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt
     8701 tgattggttt tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa
     8761 gcttttaagc ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc
     8821 gaggcaactt caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt
     8881 tgtttcccat cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat
     8941 aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact
     9001 aatttgcatc aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta
     9061 attttcactg ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc
     9121 agcgaaatcg gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa
     9181 tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc
     9241 aataccttct ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc
     9301 tccctcaaca ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa
     9361 tagtccctta gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac
     9421 cggattttca acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc
     9481 gtcaaatcca attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat
     9541 aattgcgtga acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc
     9601 aggggaaaca aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc
     9661 accaccaatc atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga
     9721 aggctcagta attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc
     9781 tttgttttta gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc
     9841 cagcattcct ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat
     9901 tggcgtcatt gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa
     9961 aaatggaatt aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc
    10021 gttcagcaac gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa
    10081 taccagcatc ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg
    10141 ctctacatag gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc
    10201 tgcagctaat accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga
    10261 aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa
    10321 ctttatggct gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat
    10381 agtattcaaa atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac
    10441 ggccaaaatg gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt
    10501 ggtagaaatg acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc
    10561 ctttttaatt gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt
    10621 gccaatcacc acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt
    10681 ttctaaggct ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc
    10741 acaatgggtt aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc
    10801 tacttctctg taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc
    10861 acgccccaat caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca
    10921 tgccaataac gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca
    10981 gggaatttaa ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac
    11041 atcaactagg gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta
    11101 taccagtaaa accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa
    11161 aaatgcatat aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat
    11221 ttggtgagta aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact
    11281 tgtttttttg gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg
    11341 tatcgataga atgatttggg ctagtattct taaaaattaa ctagcaaatg tttaaactga
    11401 tttatttcta atctacatgt agtaagtatt tcaattccac caattatcaa taaaaatacc
    11461 aaacatatcc ataataattt tttctttttc atttctttta tattgttata acagtgaata
    11521 acataaaaat tcccgacaat cattgtaata tttttttgtc acctgtatta gtatttaatt
    11581 tataggaaac tataaaaact ccctagtgaa tttacacctt tgggagttcc tgtttggacg
    11641 gtatgcataa tacatttaaa tcaatttaac attaacacct cagcatatgg tatctccaca
    11701 tatatacggt ctttctttgg atagtactca atatttatgt tgatagcact tccattgtct
    11761 aatatagctt tagcccggta tgtgttatct ccattttcat gagtaatcgt cacatcatca
    11821 catgatcgat ttaagttgta ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac
    11881 ttctctaatc cagatgtacc tgtcattttt gaaaaaataa tacctataat agccaagaca
    11941 acaataccta taatgacaaa cttcttgttc atatttactt tattcaactt tttatctcct
    12001 atctccaaaa taatttgctc ttatccctct tatcagtaca ttgacatccc taaaagtaca
    12061 ccaaagattg ctccagctac ccctgctatc atcaaaatcg tacccatttt ctcatctttt
    12121 tctcgatata agtagcccat aataacagca acagcaccaa atacaacagg aataaatgct
    12181 aacgatacta ccatagaaat ccaaccaata atcaaataca gcttccctgt ctcttgtcca
    12241 tgtacgattt gttccttcat ttcattaatt gtctgctcag tacttttctt tactgtgtct
    12301 gttccggttg tctcatctgt ttcttgtagt gctccacaaa actggcaata cttactttca
    12361 tctgttattt ctttgccaca ttttctacaa tacatgtctt ctcctcctaa aatcattcaa
    12421 ttgcttatta gttaactgtt tactttatta aatacatcag aaaacagctc attcattggt
    12481 acacttaata tctcagaaag catcttcatt tgatagggtt taggaatcgt tctccctgtt
    12541 tgccacgaac ttacacattg ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa
    12601 agtccttttt cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc
    12661 tgttttatta tatacaacaa tatgatgtat ttcaactcat aaaatataaa aaacttcaaa
    12721 taccattaca aatacaacaa cttgttgtac tatactgaga tgggggtgta ttaatgaaac
    12781 agaaacaata tgcaaaggtc attagagaaa agcgaaaagc tctcgggtgg acacaaaaag
    12841 aattagctga aaaaatcttc tctacacaac aggcagtagc aagatgggaa aattctgtaa
    12901 cagagcctaa cctcgatagc ttaacagctc tttctagagc cctaggaacc cctgtaagcc
    12961 attttctaga caatgttgtc gtagattatg aggaagagtt tttggctctc tatcgttctc
    13021 ttagcactga agatgcagtt cgaacaattg actatatgaa gctattaaaa aggcaagaaa
    13081 atgaacgcaa tcagctattg aaagattgaa aaataaataa cccaccgaac tttaacggtg
    13141 ggtgtattga gatgaaataa gattaagagc tatggataac ctgtctagtt taatacaatt
    13201 gggtcttttc tcttccttac tctccgtcca tcatctcttg ttacacttgt aaattgatac
    13261 agaggaacta tcccagctcc tgataatatc aattctttta ttgtttcatc tatccctgcc
    13321 cctttcgcta aatatattaa aaaactacta cttgctttca cattgtatag tttcttaata
    13381 tccttagaag ctcgttcaag agtgaatcct tgtttaacaa agtagcctct gttttttatt
    13441 aaagcatttt tcacgctgtt atccccaaca ttacgtaggc gttcacaaac agtgttaata
    13501 agttctttct ctgtcattag atatgtccct tgccacgata agccctgtaa aacttcatgt
    13561 gcttcatcta aaatcaattg tttttccttg ctgagttctg atcgggaatt ttgaggaaat
    13621 actcttttag cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga
    13681 tatgtaaaat gaattttttc cagaagtgga aggatatctt cattcaattt ttgaatcaca
    13741 gaagttgcat taaaaacgtc taatcgatag acattgcttc tcttatgatt aaagtttcct
    13801 aacgctttct cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca
    13861 tcatctaatt tttttatcaa tcctataaaa gtcataagtg atagtaactt attacattta
    13921 tctatgtcat aagttttacc atagtaagaa ctcatttttt tagctaattg ttcccccgac
    13981 aaaatacaag tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaagaca
    14041 tcatagacac gtcgcatttc ctctagataa ttccttattg tcctgaaaag tttgtctttc
    14101 attgattcaa gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta
    14161 ggatacaagt ctaggttctc taatagccaa ttaaatgttt ctacccgact gtctttcttt
    14221 aaaattagca tcacaactcc aataaaattt tcactgatat cacaattact ggaatgacaa
    14281 taaaaaatgg aatgcccagt tttactggta aaaatactag cagagggatt atgatcgttg
    14341 tgaaacagac atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac
    14401 aatctttcat aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac
    14461 cactgtttca tttcttcttt gtcgtcattt tttatcaact taacaaaatc ggtttcattg
    14521 actagttcat actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa
    14581 tcaatcggaa gctcagtaat atctaacatg ttgtcttcat taattattat cactgcatct
    14641 gacttatatc ctccaaaaaa cattctattc gaagcattta cattgtcatc acacccgggg
    14701 atgttctttt tcaagtattc gtaaacagca accattttac catagtcagt aattgcttct
    14761 tttaatagaa agacaatctt gtatttttct agattttcag agtaccgaat acttttttgt
    14821 atcgcaaacg cagaattatg aactaaattg atagataaaa ccttttctac gcttaagtat
    14881 ctactatcat ctttactcaa atctactacc ttccccttct catctttaac agtgttatcg
    14941 acatccagaa taattactct ggtttccaca aaactctcct tgtcattgcc tttattcttt
    15001 gtcttgcttg gaataaatga ttgtgatgaa tttagtatct ctacaaactc atctcttgta
    15061 acttctctat agttatctaa acacctatct cttacatcag ttttttcttt ttttgtaact
    15121 ggcttctcta ataatttttc ttgatctaca taaattggaa tattaatcat tgatgtttcc
    15181 atttgtgttt cctccttata aaattctgtt aaagactaaa acatacagat aataaaaaca
    15241 aactctttat tactatatgt tttagtctta tttgtttact caacctttat accgttccat
    15301 gaaactatca atatcagatt ttttaaatct aatgaccgta tcaattttta taacaggtaa
    15361 cccgtattga gaaacatatt ttctaatcgt tcctactgat acacctagat attcacttgc
    15421 ctgctgatag tccatatact ctagagtgac tccagaaata atgtcttcta gcataggctt
    15481 tagcactgta attatctctt tcacttgttg ttcaacttgt atagaaacaa tgctatcaat
    15541 gatttgtaga gacatatttc cccctccttt cgtgattgct tgaacaaatt ttctaagaat
    15601 tatcttcgta ttttttgctc aaaataaaag tattttgtta ttaaccgatt aatcaaacaa
    15661 aaaatgatta ccagttcatc gacatacaca taatacctcg ataaaaaagg ggtgtcaaca
    15721 tctttgtaaa atttatttac aacacccctt ttttgaggta tactaatttt aggaggtgtg
    15781 gtcaatatga taaaacgtcc accaataaat tatttagaac gaaaaaagat actcggaaca
    15841 aaaataaaag ctataagaaa gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt
    15901 aacaacggtc aattaattga caaaaaaact atctacgaat gggagaaggg aacctattta
    15961 ccaataccag agcgattatc ccgaattgct gatttgggaa atatgagtat agaggagtta
    16021 gtttgtggca atgtagaaga atatattttg ggaattattc tttatagaga tagcattgta
    16081 ctagatggta taacatttcc agataaaaac ctgttccagc atcttcgtca acagttccca
    16141 ccagtacact ctaatttaga tacatggctg gatagatatt ccaaattaga accagagatg
    16201 caagaattta ttgctaacaa gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc
    16261 aatatcttaa aaatagaaga attattcatc aatgctattg tagaaaaatt tgacaataac
    16321 attcttttcc tcactagcag tatagaagaa ctactagaaa gaatggtaga tgaatggcta
    16381 cctattcaat tgaaagacat gtcttatccc gaagaagctg tgagagaaat tactgataat
    16441 ataaacaagc tagaacaaac gatttcatca attgggaaaa aatacactaa gaaaaaaatg
    16501 aagggaggtg ataccatcta gtggtaagta ctccttgtaa actaagagga gatgatttaa
    16561 tggcaaaaat atcaaagtac aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac
    16621 acattgccac cgatccacga actggtgaaa aaataaatac tacgagaaga ggttttagta
    16681 gcaaacaaga agctcaaaaa gattatgacg agtacagaca tcaaatatta tacggtgtta
    16741 aaaagaaagc acctgatatg acatttgaag acctgtataa tgaatggata actcatcagc
    16801 gtaccagtgt taaagcttca acaatcgcaa tctcagtaag atacgcaaat aatcaaatac
    16861 ttccagcatt tggtaagctt aaactatcga atatctctgt cccatattgt caaaaagtgg
    16921 tagatgaatg gcacagtaaa tatgaaagtt acgattatat gagaaaacag actgcacaaa
    16981 ttttacgcta cggggttgca atgcaatata ttgacaataa tcctatggaa aaaacacttc
    17041 ttccacgaaa aaaagagtat gagaaaaatc gtaaatttta ctcaaaagag gagcttaata
    17101 atttactgga tgcttttaaa gattttggta acatgaaaca atacgccttc ttcagattat
    17161 tggcatacac aggtatgaga aagagtgaag tactcgcttt acagtggaaa gacatagaca
    17221 cgtttaataa agaactacat gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta
    17281 taattcaatc tccgaaaaca agagcttcta gacgagttat ctctttagat actgagactc
    17341 tttctatcct caacaactgg aaattacagc aaaaagaaga gtatttaaaa cttggataca
    17401 atactagctc taaagagcaa catgttttta caacagttaa aaacactcta tacattccaa
    17461 acactgttaa tgattggtta agatatattt tgaaaaaata taacttgcct cgtattacac
    17521 ctcatgggtt tagacataca catgctagtt tattattaga agcaggagaa tctgtcaagg
    17581 tagttcaaca acgattaggg catgaaaata gtaaagtgac attagacatc tatgcacata
    17641 ttacaaataa tgcacctaag aaaacaggtc aagactttgc tgatatgatg gctcatcagt
    17701 aaaagagctg gtcaaaatct ggtcatttct agtatacagg caaaacaaaa aagctagaac
    17761 ccttatatat caagggttct agctcctata acaggtttac ccaacactgc cttccatctc
    17821 ataactgatc aatcgattga gttcgactgc atattccatt ggaagttctt tggtgaatgg
    17881 ttcaacaaag cccatgacga tcatttcagt tgcttcagat tcggtcaggc ctcggctcat
    17941 cagatagtag agttgttctt ctgagatttt tgagactttg gcttcgtgtt ctaaagagac
    18001 ttggctgttg tggatctcat tgaatgggat cgtatcggat ttcgaccatt cgtccatgat
    18061 gatcgtgtca cattcgatat gagagatcga acccgcactg tttttggcaa aggttacttg
    18121 tcctcgatag tttacttctc cgccgtcttt tgaaattgat ttagagacga tagagctaga
    18181 tgtatttggt gcattgtgga tcatttttgc acccgtatct tggatttggt ttgctcctgc
    18241 aaaggcgatg gagagcatgg tgcctcgtgc accttttcca tctaagtaaa cacttgggta
    18301 tttcatcgtt gtttttgcac ctaggtttcc gtcgatccat tcaacggttg cgccttcgta
    18361 tgcttttgct cgtttggtta ctaagttgta cacattgtct gaccagtttt ggatcgttgt
    18421 gtaacggcaa tacgcatctt ttcttgtaaa gatttctacg atggcggcat gcaggctgtt
    18481 acttgaataa gtaggtgccg tacaaccttc gacatagtgg atgcttgctc cttcgtcaac
    18541 aatgatcagc gtacgttcga attgccccgt gttttccgca ttgatccgga agtacgtttg
    18601 taatggaaca tcgactttga cggcttttgg cacgtaaata aaggtcccac ctgaccatac
    18661 ggctgaattc agcgctgcta atttgttgtc ggttggcggt acaagttttg aaaagtactc
    18721 tttgaataat tcagggtatt ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa
    18781 cttttcgaat tcatctttca tattatggta cacgacttct gattcatatt gtgcggaagc
    18841 accggctaga taagctcgtt cagcttctgg tataccaatc ttttcaaatg tatctttgat
    18901 tttttctggt acgtcttccc agtcacgagc gggtttgtca cttggttttt ggtaatattt
    18961 gatggcatcg aaatcgatgt ctgaaaggtc ggggccccat gtttgcattg gcattttgtt
    19021 aaatgtttct agagatttca gacggaattc aagcatccat tctggttcgc ctttgattct
    19081 tgacatctca cggatcactt cttctgtcaa gccttctcct gtactgaaaa ccggttcgac
    19141 atcatcgtga aacccgaatt tatattcttc taattctggt acgcccatgt tctcactcct
    19201 cttccttgtg gtaatgatgg aaagctgcgg taccatcgtt ttccattgct tttcctagtg
    19261 ctttccaggc aagtgtcgca catttgatcc gagcgggaaa tttagctaca ccaccaagca
    19321 tagcagcatc gcctaattgg tcgggatctt tgacttcttt tccttggaca agctgtagaa
    19381 agtctacagt taaatgttct gcttcttcta ttgattttcc gatgacggcg tctgtcatca
    19441 tcgaggcact tgccgtactg atggagcagc cactgccgtc aaaggcgata tttttgatga
    19501 ttccgtcttt tacatcgact tgcaattcga tcacatctcc acatgtgggg ttgttcaatt
    19561 cgatcttgct tgtcgagtca gtcaagcttc cacgatgatg cggatgggaa gaatgatcca
    19621 agatcacttg acggtataaa ttgtctaatt tagatagtcc catgttggaa aaactccttt
    19681 gttgccatga ttgcttcaac taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag
    19741 ctcgcgcgag cagtcgctga tacttcaagg tacttcaata gcggttgcgc acaatgatga
    19801 cctgctctga cggctacccc ttccatgtct aaagctgtcg cgacatcatg gggatgtaac
    19861 ccatccaaat tgaatgcaac gactcctgtc cgaaaattcg ggtcttgtgg accataaaca
    19921 gtcaatccat caattgcttg taattttggc aacacataag cggctagttc ttcttcatat
    19981 cgatggatat tgtccatacc gagtttttgc aaataatcga ttgcatggcc taatgcgata
    20041 gcgccggcga tatttggtgt ccctgcttcg aatttccaag gtagttcttt ccatgtactg
    20101 tcgtataatt cgacaaaatc gatcatttcg ccaccaaatt caacaggttc catttcttct
    20161 aaccattttc gtttaccata taaaacacca atacctgttg gtccgcacat tttgtgtccg
    20221 ctgaaagcaa agaaatcaca gtctagtgct tggacatcaa tggccatgtg cggggcagat
    20281 tgagcaccat ctactacgat gaccccgccg ttttcatgga caagttcagc taattcttga
    20341 acaggattga agacacctaa gacattggat acatgagcaa tggagacgat tttggttttg
    20401 tccgtaatct ttttcgctgc atcctccata tccaggaagc cttcttctgt tagttcgata
    20461 tatttcaaga cagcacctgt tcgttttgcc aactgctgcc acgggataac attagaatga
    20521 tgttccatgt aagagagcac tatctcgtct cctgctttga tatagcggtc gccgaaggtt
    20581 ctggctaccc aattcaagct tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct
    20641 tttgcatgga tgaacgcccg tactttttct cttgcttgtt cataatcatg ggtcgcgcgt
    20701 tctgctaacg tatggacacc gcgatgaaca ttggcgttat cgtgttcgta atagtacgct
    20761 aatgcctgga gtacttgttt tggcttttgg gtcgtcgctg cattatccag ataaactaaa
    20821 ggttcatcat tgacgtcttg aaataagata ggaaaatcat tgcgtagctt tttagcatcg
    20881 atcatccatt caacttccct tcgattactt cgactaattc tttccgaact tgttctacag
    20941 ggattgctgt taagacagaa ccgaggaatc ctcgaatcac taagcgctct gcttcttctt
    21001 tatgcaatcc tctactcatc agatagtaca tttcttctgg atcaactcgt cctacactgg
    21061 cagcatgtcc agcagttact tcgttttcat cgatcaaaag aataggattc gcatcaccac
    21121 gtgctttatc agaaagcatc aagacacgac tttcttgttg ggcatctgca ccttttgccc
    21181 ctttcaagat gtggccgata ccattaaagg tcaatgttcc tttttctcgg atcactccgt
    21241 gttgcaaaat atgcccgatc gtatgagggg ctttattggt tactcgtgta tcgattcctt
    21301 ggatttgtct gcccgagctg attgccacga ttttgacttc tgcgtgtgca ccttctcctg
    21361 ctaaatctga gtcaaagtca gcgatcacat gaccgtcatt catgacacca attgcccagt
    21421 cgacagaagc atctcgtaaa atgtgcccac ggcgattcat ataagaagta atattttctc
    21481 ctaattgatc tacggctgaa tatttgattt ttgaaccagc tttagcgatg acttccacga
    21541 tgatattacc tgatgatttt gctacttgtt ctttggttgt ctggaaacgt tccagataag
    21601 aaaattcact atgttcatct gccacgatca acacatgctt gaagaagtgc tcatcggatg
    21661 cgccatcttg aatgaataag gactcgagtg gttcatcgat cacgacattt ttaggcacat
    21721 acaggaaaat accgctgttc ataaaagcta catggagtgc agtcaattgg tcttcgttca
    21781 tgtttactgc ttttttcata taatattctt ggactagttc tggatattcg atcattgcag
    21841 tgaacaagtc ggtaaaaatg actccttttt ctgctaattc cacaggcagt tgttcaaaaa
    21901 tcgtccatga accttgttgg acaagcacag gattgtcttt cattgcatca aatgccggaa
    21961 tcgtgcctgt ttcactgatg gtttgtgttt ctttcacatc aaataacgac cagcgatgga
    22021 atttcacccg atcgatatgc ggcaatggca gttcatctgc tttcgctagt gcgtcttgac
    22081 ggaaggttct catccaatca ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa
    22141 gataatcgag ccagtttttt tctttcattc ttgctccccc ttacgcttct tcttctttat
    22201 agtcgattcc taattcttta ctgataccag cgtagccttc tgcttccaga cgtttagcta
    22261 ggtcagcatt ccctgtcatg actactcggc cttccatcat gatgtgaacg acatcaggtg
    22321 tgatatagtt caataatcgt tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc
    22381 gcatttcatt tactccttta gcaacgactt tcaaagcatc gatatccaga cctgaatcga
    22441 tttcgtctaa aattgcaaat gtaggttcta acatcaacaa ttgcaggatc tcattccgtt
    22501 tcttttctcc gccagaaaaa ccttcgttca gataacgttc agccatttct tctggcatat
    22561 ttagtaaagc cattttttca tctaactttt tcaaaaatgc cataacagaa attttgtcgt
    22621 tttcatcacg tttcgcattg atcgctgcac gcatgaattc cgcattcgtg atacctggta
    22681 tctcacttgg atattgcata gctaaaaata agccaagtcg cgcacgttca tctacttcca
    22741 agtctaaaac atttttcccg tcaaacaaaa tttcgccttc tgttacttca tagtttggat
    22801 tgcccatgat agcggcagat aaggtagatt ttcctgtgcc gttaggtccc atgatggcat
    22861 ggatttctcc ggttttcatt gttaggttta cgcctttaag gatttctttt tcctcgatcg
    22921 acacatgtag attttttatt tctaagacag acatgcatta tacccccaaa ttctctttcg
    22981 ttctcactca ttttagacta attgagaatg aagcgcaatg accttcctta ctaaaatcaa
    23041 atatctttta tttattatta aacaattttg atttctaaat tagaataggt ataaaaaaga
    23101 tgagaaataa gccattacag acttatttct catctttttt gttccatacc ttattttgca
    23161 tcagccttta ctggtttaac agagccgccc catttgtctg tgatccattc ttggacttct
    23221 ttgcggtgaa gcacttcaat cagttttttg attttgtcac tgtcttcatc acctttacga
    23281 gtcgcaataa tattcacgta aggagagttg tccttttcaa gcaatacggc atcttctaat
    23341 ggattcaacc ctgcgccgta agcgaagttt gcgttgattg caactaggtc accttcatca
    23401 ttttggtacg tagaagctaa aagtgatggg tcgatcgtat ggttgaactt caagttctta
    23461 gggttttctt caatatcatc gaatgttgct gtttccagat ctactccatc tttgactttg
    23521 actaagccag catcttgtag aattgtgatg atgcgtcccc agtctgattc agaatttgaa
    23581 gtaatgattg ttgcaccatc ttttaattcg gaaacatctt tgactttttt agagtacaag
    23641 cccattggtt cgatgtggac agcaccggca ttgacaaagt catatccttt ttctttgatt
    23701 tctttgttca aataaggaat atgctggaag taattggcat caatgtcgcc ttcttccaaa
    23761 gattggttcg gtaatacata gtcatcgaat ttgacgattt caagatcgat tccttcatct
    23821 ttcaataatg gtttcacatg ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact
    23881 ttcaatgtcg tgtcgtctgc tttatttgct gaatctttgc tgtcgctgtt gttaccgcat
    23941 gctgctaatc ctacagtcag caaaagcgct gctgcaaatc caaaaatttt ctttttcata
    24001 tcctacatcc ccctatatcc tattatcttt tgtctgtttt cttagttaag aaatcaccga
    24061 ttccttgaac gataaatacg attagtaaaa taattaaagt cgcaacgaat gtcactgtta
    24121 gattaccacg ctggtagcct tcctgccaag caagacctcc aagtccacct gcgccgatcg
    24181 cgccggccat cgcagtaaat ccgatcatcg agatcgttgt aactgttaat ccagaaatca
    24241 atgcgggaat actttcaggt atcaagacct tccagatgat ttcccagtaa cttgctccca
    24301 ttgcatctgc tgcttccagt acaccagggc tgacttcacg aaaagcaatc tctactagac
    24361 gggcataaaa tggtgctgcg gataaaacaa gagctggaat ggctgcttgc gcacctagca
    24421 tagtgcctac gattgcttta gtaaatggaa tgatcaaaac catcaggatc ataaatggcg
    24481 ttgaccggaa aatattactg atgatcgata cgattccata aatgattcga gcatacggtt
    24541 tgtttttgcg tccaatagag taaaggatca atcccaaaac taatccgatg atcgtcacta
    24601 agaccattga gattaatgtc atatatagtg tatcaaggat agcctgattc atcgtttccg
    24661 ggttgatttg tttaaaatct agatatgttt ctgaaaagct tttatccacg atggatcacc
    24721 tcaattccta ctccttgttc tttgatgaac acttctgctt gatctaattc tttggtttca
    24781 ccagtcatca agacagtcaa tgaaccaaag gaaccttctt tcacttgttt gatctttcca
    24841 tacacaacgt tgacatcgat gttatagcgt cgaatcgctt gggaaatgac tggctcgttg
    24901 gcatttcttt cgctaaaggt caaagttgcc aaacggccgc ttggattttc tttgatgagt
    24961 tcagctaata gttctgtcga atcagcttct ggttcgatgt cttgttgtac aaagcgtttg
    25021 gttacttctt ttttcggttg tctgaagaca gtcaatacgt ctccttcttc tacgactttc
    25081 cccaactcca tcacagcgac tttgttgcag attttgcgga tcacattcat ttcatgtgtg
    25141 atcaagacga tggtcaaatt caatttctta ttgatcgcta acaataagtc taatacttct
    25201 tctgttgttt gcgggtccaa tgcgctagta gcctcatcac aaagcaggat ctcaggatca
    25261 tttgccaatg ctcgtgcgat accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga
    25321 tatgcttgtc cgcgtccttc taacccgact aaacgcaaca gttcttctgc ccgttcttta
    25381 cgtttgctct ttggtacgcc agctaattcc agcggcagct ggatattttc aagtacagtt
    25441 cttgaccata acaggttgaa atgctggaag atcataccga ttttctttcg gaatgtacgt
    25501 aattctttgt tttttaattt tgtaatatct tgatcgttta cgatcacttc gccgctcgtg
    25561 ggaagctcaa gtccattcag gagacggact aatgtacttt ttcctgcgcc agaatagccg
    25621 acgattccat aaatatcccc tttttcaacg tttagtgaaa cgtcattgac ggcttggatg
    25681 tttccgtttt tcccattgaa tgtctttttg acattcgtta gttggattaa tgacatgcca
    25741 ttcacatctt tctcttatga tttttcaaac aaaaaagcct tcgccctctt gccttaagca
    25801 aaaggacgaa agcttgaact tccgtgttac caccttgatt taccgttact tcgcaataac
    25861 agcctcttcc agtacttgga cttagcttct ttccgtatac tgtcgcgctg taacgggcac
    25921 tcccgtagta agtttgctta cgctcgctta ctctgctcta agaccatctt ccatctcatt
    25981 ttcgttaccc attttcagca aacagggctc tcttcaaacg tccgtgacat gtactcttct
    26041 tttcaaagct ttctcgtttc atataggatt gcttatgatt atagcaactg gaagaatcag
    26101 tgtcaacttt tttcaagtga ttttctgatt ttataattcg tcaaattctt ttggatcaac
    26161 atctttgaaa cttaagatcc acgcattcat ttcatctgaa tcattcaaga tattgatatc
    26221 ttcaccgatt ttatcattaa cagaagaaac tgtacccgtt agcgggctgg caaattcatt
    26281 gaccgctttt tccgcttcaa cttcgatcag aggttcatct ttttttagcg attgtcctgc
    26341 ttttggcaga ttggcaaacg tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc
    26401 aacacagtat tcttttccat taaataagat ccaaaggttg tcctttttct tcaaatgctt
    26461 gcctgccatt ttatatcagt tctccttcat aaattttttc attgaatcca attgcaacaa
    26521 gctggtcctc tcgtaagatc agtggtcgct tgatcagcat accatctgtc gataagactt
    26581 ggctcgcttt tgaagccgta aactcgccta cttgatcttt cagtccaagt tcccgatatt
    26641 tcatcccgct tgtgttgaac aggcgacgga ttggtacttg actcgtttct agccaagtct
    26701 caatcgttga ggccttcggt acttctttga tcatatcaac ggtttgataa ctaacgccat
    26761 gctggtctaa ccacgctttg gcttttttgc atgttgaaca tttcgggtac cagtaaaagg
    26821 tgtacaattt tcttacctcc ttgaagatct tgcaggtgct tcattttggt atctcatcga
    26881 taatatcaac ccgataccga tcatgttccc taaaatcgag gatcctccct gactaatgaa
    26941 aggcaatggg atccctgtta aaggaagtaa tccgatattt gctccaatat tctcaaagac
    27001 atggaacagc agcatcatga tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt
    27061 atcgaaacac acccgaatca tacgatagat taaaataaaa tacaagaaaa tgacgaatgc
    27121 accaccgata aagccgaagt tttcaccgat aacggtaaaa atcatatcgg actcacgaac
    27181 aggcacatag atattgctta cgttgaatcc tttaccaaac aagccaccgg taccaattgc
    27241 caaaagtcct tgggccggct gataactcat tcctgacgta tcatggaaag ggtccagcca
    27301 tgaatcaata cgtgcaaact gataagattt gaatccaact ttataaagca agtctcttcc
    27361 gccttcagtc gtaaccaaga agatcgttcc tgctccaacg actactgcca agattactac
    27421 aggtgcgatg atgcgccaag agattccaga catcaggaaa atcccgccaa agatcgcaag
    27481 aaagacaagc atcgttccaa agtcatcttg ggccatgatc aatgctaaaa ccggtattgt
    27541 cacgatcaag agcttcccaa tcagccaaaa gtccgatttc atcgtccgtt cttttacttg
    27601 cgtattatgt ttagttacga tcagtgccat catcaagatg tacgcaatct tcatcaactc
    27661 tgctggctgg aaagtaaacg caccgaatcg aaaccagttt ttagaccctg ttgaagcagc
    27721 taaattctga tcatagaatt tgagcaagag tcccattacc actaacccca aaccataaag
    27781 gtaaggagtc agtttccaga tccatttgga attcaaatgc atgatgacaa cgattgctgc
    27841 cgctccaact aaataccaga caacttgcat ccctacgcct ttcaagactg ctggattgtt
    27901 cggatcatgg ctaagtgcta catataatga gagcagacca atcaaacata acagaaagac
    27961 tggtaaaatc acgccataat caatacgatt atcgttcgaa gcttttaatt ttttttccat
    28021 gtttcatccc ttttcttaaa aaccatactg ctgattatta tagcggtcca gttagataat
    28081 ggaaagtctt tccatagaca tttgggaaaa attaactttc cgtttgtgca gttgcttgag
    28141 caaatgcagt acaaaagcct actgcttatc tttagtgtat catagaactt tttttgatac
    28201 atgcaattat ctaccagctg agattctcct cgctgtgaga agtttctgtg ctttcattgt
    28261 atcgaaggat ctcttcggca atctctagca gctgatcaga aaccagattg gttcgttgga
    28321 tgaaataaaa atatcgatgg aaattcaacg gagtcattgg cacattttct ggaacagagc
    28381 gcttagacaa aatcgagcga ccgattcctt ccttcagcaa cgcaacaatt acatcgttac
    28441 ttttcacgat catcttctta tcttggatat tcatctcttc aaaataacgc tgcgtgtaat
    28501 gaaaaacacc agagtttgct tctcttacaa gccacaattt gctttttggg tccccagcga
    28561 tgactagctg gtcttccatc aaagagtatc tttgtacgcc ggaagtctct aatggctttt
    28621 cgataaaacc aaaatcggat tcatgtttcg ctacttgttc taatgattca tgagaattcg
    28681 tgatttcgat ctcaaaatgc actttaggaa accttttgat cagatgtttc atcaactgag
    28741 gcaaaagata gacagcaaag gtgtgggaag caacgatccg acatacttct tcttcatttt
    28801 gttcattgag caatttttgc tgagtttctt cccattcttc taacaaaacc agtgtccgtt
    28861 catataataa gtcggcttgc ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa
    28921 gatcgatatt caattcccgc tccaactgtt tgatttgatt cgagactgct ggttgagata
    28981 aaaacagtac ttctgctgct ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg
    29041 ttaataactt aaacatggca ccacttccat aactttttat gataattact ataaaaacta
    29101 tttgatttaa atatacaaaa aaccatctat aatagaaatg tacaacaaaa cagttgctga
    29161 tccaactagt aacttagatg aagacaatca gctgtctgct tgctcgtaat catacattcc
    29221 tattcatttt acaatgtatt cgttcatctt ggtactaaaa tctattaaat aaaaaggaga
    29281 atattatata tgaagaactt tttgaaaatt attccgatcc cgatctgcgg gttgatcctt
    29341 gggttagctt cccttggaaa tctgtttaaa gactatcacc atgttgcttt aggcaatcta
    29401 actggcggta tctctatgat tcttatgata ttcatttttg ttaaattgtt tatcttattc
    29461 gaacacacga aacaaacgtt gcatgatcca atcatcgcct ccgtttcacc aacttttacg
    29521 atgtcactca tggttatttg tacttatttt gtttcatatg aaagtattgc accaattgtc
    29581 aaattcattt ggctgtgcgc tgttatcttc caagtgatcc ttgtctttta tttcaattat
    29641 caccatgtta tcaaagctga tctttcaatt gaagcaattt atccaagctg gtttatcatt
    29701 tacgttggat ttggggtcat cactgtaact gccgggaact tcttcccact tattggaaaa
    29761 attttctttt ggatttcatt gatctgctat gcgttcttat tgccgatcat catcaaacgt
    29821 gtctttttcg taagaaatat ggctcatccg acacgtccgc tgattacgat cgttgcagca
    29881 cctggttctc tttgcctaac aggttatctt aaaaactttg ctgaacctaa tttttatctt
    29941 gtcgtagcat tgttcgcttt atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca
    30001 cgtttgttca agcttcgttt ttatccaagc tatgcagcct ttacattccc gcttgttatc
    30061 tctgcaacag ctatatttac tactgttcat tacttcaaca gtttaggtat ctatagtagc
    30121 gtattggatt tcttaaaagt tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt
    30181 cttgctcatt acgtacgttt cttaattaaa gaacatagca aacatggaaa atttgtttct
    30241 aaaaaaatcg cttaataatc gattcaatag gttgtgaaaa agctgttctg catcaagtaa
    30301 taagaacggc caaacaaaaa cagctgttca tgtttttgta ctggttgggc ttattaccgc
    30361 agatgcaagc ttttgaacac cgtttaccaa gaggtcgtaa aagaagtggt cagcttcaag
    30421 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatttc ggcttatttc
    30481 cgtagaagct acttctggaa caccgtttat tcgaaacaag aggtcgtaca agacttttgt
    30541 cacgacctct tgttcttttg tcaaaatact ggtttagtgc tactatgttc gagtatgctt
    30601 ttagtattta ttgacgaaag gaaccgatca aatgaaatca ttgtcacatc caataaaatc
    30661 tgtaaaggat tcagcggtcc gcgaaatctt acccggttta ctgctttctt ttgccatcgc
    30721 tgccttcagc aggcttcttg ctatttatct gccacagtta ggtggagcga ctattgctat
    30781 tttattgggt attttattag gaaatacttt ttttaaacag cctgttctag caatcggcac
    30841 aaaatttgct gaaagcaaac tattagaatt ctctgttttt ttactaggtg caactgtcac
    30901 agctcaaacg attgctcaaa ttggtgtaaa aggaattgtt tttgtgttac tgcaaatgtc
    30961 agtcactatc atcggtgctt acttgattgg taaaaaactg caattcagcg atacttcttc
    31021 tttgatgatg gctggaggaa acgctgtttg tggttcttct gctattgctt caatcgcccc
    31081 ttccatccat gcgaaagagg aagaaaaagg acagatcatc acactagtca atctactggg
    31141 tactgtgatg atgttgactc tcccttttct tggattacag ttgtttggtg gaaatctcat
    31201 tgaaaaaagt gcacttttgg gtggtacact ccaatctgtt ggtcaagtag tcgctggggc
    31261 aagcttactt gacaattctg ttgtcgaatt cgccatgcta tttaagattc tacgtattat
    31321 gatgctcgtt gttgttgtct atctgtttga aaaattcatg cacaagaaac aaccagctct
    31381 gtttgaaacg ccaactgata cgcagaaaaa acgaaaatta ccgcttcctt ggtatgtatt
    31441 agggtttgtg atcttttgtt tggtcaacag cttgttttct ttacctgtag ttattgacca
    31501 atcggcacat tttatcagca gctggtttga gatcactgct ttagcggcaa ttggtctgcg
    31561 tttagacttt gcaaaattca tgaaagaagg tacacgcttc ttatcttatg gattagctgt
    31621 cgggcttctc caaacagtga tggctttact atttatttgg ctgatcaaag tttagaatga
    31681 acatgaaaaa acagttgtga ttctcgcatg gaacaatgtc cgaatcacaa ctgtttttaa
    31741 ttttgcagat atccttctaa caacaacaat tggcgtttga tttcaacccc atctattgcg
    31801 tttcccatat atcctgtcag ttttccattt tgcccgatca ctcgatgaca aggaataacg
    31861 attggcaatg gattggttcg atttgcctgc ccaatcgctc gcacagcttt cggactatca
    31921 acagcaacgg ctacttcttt atacgttcgg atttctccat aaggaatggt cgacaaagct
    31981 ttccagacat tttgctgaaa aggcgtacct ctctttatag ataaaggaac atcaaattct
    32041 tttctttttc ccgcaaaata ttcttcaaac tgttcttgtg tgcgcgttaa aattcgctga
    32101 ttcgctgatt cgctttctga aattggctga aaagatacag cagtcaatcc ggattcatct
    32161 gcatctaacc ataaaaagcc gataggtgtg tgtattttca ttttgtttgc tcctagtcaa
    32221 tagactggaa cttcctcctt tttctctatc caaaactact tgccaactaa taaaaattgt
    32281 tcttaaaagt ttgataaacc gaggaaacat ggtaaactaa atacgttagt ttacaaaaag
    32341 aatacctgtt cttttacgta aaatcaaatt ttaaaggtgt tatttaaggt ttattaaatt
    32401 ttgaatgtaa accatatata aacatacgat tgaaaggata gaaacgatga aagtatcaat
    32461 tgttgtccca tgttttaatg aagaaaaaac tgttcctctt ttttttgatg aggttgaaaa
    32521 attcaaaggt gcccatgagt tcgaatattt gtttattgac gacggatcaa aagacggtac
    32581 gttagcagca atcaagaagc tagcctcagc ttatccttat gtccgctttg tctcattttc
    32641 cagaaatttt gggaaagaag cggctctgta cgctggattg caagcttcta caggagattt
    32701 agtgaccgtg atggatgttg atctgcaaga tccgcccgaa ctgcttcctg agatgatcaa
    32761 ccggatcact acttctgata tcgattgtgt cggcacccgg cgttcgactc gtgaaggcga
    32821 gcctgcgata cgcagtttct ttgcgaaaaa attctaccag ttgatcaata aaatcagcga
    32881 tacagaaatg gtcgatggcg ctcgtgattt ccgcttgatg actcgtcaaa tggtggatgc
    32941 cgtcctagaa ttaacagaat acaaccgatt ttcaaaagga ttatttagct gggtcggatt
    33001 taaaacagaa tatatttctt atgaaaaccg cgaacgtgtg gctggcgaaa catcttggtc
    33061 cttctggaaa ttattcaact actcgattga tggtatcgtg aacttctcgg atgcaccgtt
    33121 aacgattgct tcatttatcg gcgctttgtc ttgtgtcggt tctggtattg caattttgtt
    33181 cattattttg cgggcattgt tgtttggtga tcctacatca ggatggcctt ccatggtctc
    33241 gatcttcctg ttcattggtg gaatccaatt gttatgtctt ggaatcatcg ggaaatatat
    33301 tgggaaaatc ttcttagaaa cgaaacagcg tcctgtttat atcataagag aaacagaaaa
    33361 agtgaagcca aatgaattga agcctcacca ataatatacg tcaaatgaag taaacatttt
    33421 tttaaatgaa aaaggactgt gacaaaagtc ttgtcacagt ccttttttcc gaataaatgg
    33481 tgttccagaa gtagcttctt cggaaataag ccgaaatttc acaaaaattt gagaagcaat
    33541 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tatcacaacc tctttcatat
    33601 cttattactt cttcattgct gcttttagtg ctgctgctaa tgggctttct tctggttctt
    33661 ctgcttgtgc atattttttc agcaagcgtc gttcttcatg tttggtcatt ttcttcttct
    33721 gctctttttt atcaagcaat ttttctgtcg ttccatcgta tttacaacgg aaataagcac
    33781 cgtttttacc ttcgatgatt tccattttcc ggtgacattg cgggcatcga tgattagaga
    33841 cttttggatc ttttcttctg cggtagttgc aatctgaatt ggtgcagaca tagattttgc
    33901 cgtcacgtgt gtttttctcg cgcagattgg ccccacattc tgggcatttc ttttgggtaa
    33961 tcgagaaatc ttgatattct tgtttgcttg atttgatctc attcaccaag cgttttgttt
    34021 ctttttcgat tccattcaag aaggttcctg cttgtttctt tcctgcagca atagcttcaa
    34081 ggtctttttc ccagctttct gtcaaatctg gtgttactaa agagggattt accaatacta
    34141 ataattgctt acctttagga gatacttgca gtccttgagg ggtacgttcc atcagttcag
    34201 atttgattag tttctcgata atctctgcac gcgtagctgg ggttcccaga ctgtattttt
    34261 ccatttgacc aagcaagctt gcttctgaca atggtttagg cggtgcagat agttcttttt
    34321 tgatggcgaa atcgggattg attctcattc ctttttccca tttgactgtt tccgtctcag
    34381 tttcagtttc gcctttccat cctgcttgga cgacacgatt ttgccgaaaa atgaatcgtt
    34441 ctttaccaaa ttcaaccgta acttttgttt ggctgacttt atggggttga gcaaacagtc
    34501 ctaaaaaacg agagacaatc atttgataga tttttcgctc ttcattactt aatttttcat
    34561 aacgtgctct gttttcagtt ggcaaaagtg catggtgatc ggtcacttta ctgtcttgga
    34621 atacttttgt ctgttggacg atggctccgt ttttcaaata ctgcttgacc tcaggagcaa
    34681 agtcgctgac tgcttgcaat cgttctttca tcgtactttt catgtcattt gtcagatatt
    34741 tgctgtctgt acgcggataa gtaacgatct tatgggtctc atacaaactt tgtacaagac
    34801 ttaatgtttt ctttgcagaa tagccatagc gttgattggc ttctctttgg atctctgtta
    34861 aatcataagg caatggcgca gattccgttt ttaatttttc ttggacttct gtgacagttc
    34921 ctttgcttgt actgatcaat tgaacaagtt tttgtgcttc ttcttgtgtt tttaaagcaa
    34981 attgattttt ttgaagcatt ttggcttgtt cttttccagc ttgcaacgtg atcgtgaaat
    35041 acgtttgtgg acggaactgc tcgattttct tttcttggtc tctcactaat gctagtgtcg
    35101 gtgtctgcac acggcctgct gataaattat ccgcgtactt cacagttaag gctctggtca
    35161 cattcaatcc taccagccag tccgcttttg cccgggcaag agcagattca tacaatgcat
    35221 catattcttt cgcaggtctt agttgcttga atccagtttt gattgcttta tctgtttgag
    35281 aagagatcca taaccgcttc actggtttat tgaaatgcac atactctaag atccagcgag
    35341 caacaagttc cccttctctt ccggcatctg tcgcaatgac tgcttctgag acatcttttc
    35401 gctgagccag ctgcttgatt gcttttaatt gatgtcctgt tttaggaagt ggtttgattc
    35461 ctagtttttt agggatcatt ggcaacgtgt ccatttgcca gctctgccat tccttattca
    35521 gatcttccgg cattttcaat cctaataaat gaccaagggc ccaagtaacg atcaccttgg
    35581 gtccttcata ataatttttg tgcttttgat tagcaccaag caccttgctt aggtctttcg
    35641 caacgcttgg tttttccgct agaattagtt gtttcataaa attcctttct gctttctact
    35701 gtgatgggct gatgattttt caacaacgca agtcccttgt attgctgtaa atcatcgtca
    35761 cactcttcac accagcgctc atctccgtca ttccaaaagg cgctgagaaa gttacttttt
    35821 gcattgatat attcataatt ttgatggatt tcgttccatt gtttttcgta cgcttgtcgt
    35881 gcttcttcga aagactcgaa tgtttcttct tcttcaatat cttcttccca gtcttcaaaa
    35941 aaccaccaag gctcattgtc cccgaacatg gtaattactt gatacatact cgtgctcctt
    36001 ctttcagctt ttctagactc atcttagtca cttttcaaga aagttgcaag aattttctct
    36061 tataaaaggg tcatttaatg aacatcttaa aattgttaca aatgaatctg attgtttaag
    36121 gcaaagtagc gataaagtta ttgatcatcc aaaaatagct gtcttctgga tcgattgttg
    36181 gttcttttgt aaagtatcct aaaaaacgta aggaaagata accgtggatc atactacgaa
    36241 gttccctgct tcgatgaact ttttcttctt tcgttaattg aaaagcctca aatatctgca
    36301 aaatgatctg attcgtttca tggattccat ctaataaaac agcttcattg gtatgcggta
    36361 cactgatcag taactcatac actgcttgat ttgaaaaagc aaaggatcga taagtttctg
    36421 catagatctg cagtgcttcc tgaccacttt ttcccacaag agcccgtcgc agctggtcat
    36481 taagttcctg cagaagtttc gctgttagcg ctgttttgac atcacgaata tttttgaaat
    36541 ggttatacag cgagggagcc ttgatatcca agcgtttggc taaatgctga aaggttattt
    36601 gctgaacatc catctcttct gctagttctc gaaagcagtc gatgattttc tcttgagaca
    36661 gatttctttt tttcatttcc atgtttcctc tcataaaata aatccttcac tctttcagtt
    36721 taccacgaaa aagaaagaga aaaaagagac tttcattgat aaactacata ttgtagttta
    36781 taataaagag ctgtagatga aaattttcag aaagaaggag tctaattggc tgcaagaata
    36841 aaggaaaaat tatttaggaa aagagaaact tgggaacaaa acttgtttgt cttatggttt
    36901 ggaacattta tggctggtat cggatttagt ttagtcatgc cttttatgtc actttacata
    36961 gatacgttag gtaattatag tacgtcacaa ttgaactttt ggagcggatt gactttttca
    37021 tctactttct tggtgactac gcttatctct ccttggtggg gaaaattagc cgaccaaaaa
    37081 ggaagaaaat tgatgctgtt gcgtgcttca ttaggaatgg caatcgtcat ttctttgatg
    37141 ggttgcgtga ctagtgtgta tcaattagtc gcattgcgtc tgcttcaagg gattttttct
    37201 ggttatatca gtaatgccac tgcccttgtc gcaactggga cgccaaaaga aaaaagtggt
    37261 caagttcttg gaaccttagc aactggttct gttacaggaa cattacttgg accgcttctt
    37321 ggcgggatct ctgcttcagc gtttggctat cgccctacct ttttcattac agggatcatc
    37381 ttatttttcg tcttcttgtt gagtctgttt ttcgtccatg aaaaatttgt cccagtgaaa
    37441 aaagaaaaca tggtttctgc caaacagatt ttcaaagact tgaaatatcc acacgtcgtc
    37501 atcggtatgt tcgtcacaac gatgatcatc caagcatcca acaattccat tagtccaatc
    37561 atcagtctgt acattcgcca attgctgcat ggacatggga atgttacgtt gatcagcgga
    37621 atcattgctt cgatacctgg gattgccact ttgatagccg ctccaagatt tggtcgctta
    37681 ggtgacaaga ttgggagtga aagaatactt gcgattggtt tgggttttgc gattctggtt
    37741 tatatcccaa tggcattcgt cacgaatgtt tggcaattag ctgccttacg ttttttgatt
    37801 ggtatctctg atgcttgtct gctccctgct gtccaagcat tgatcacaaa atattcgcct
    37861 catcaagctg ctggacggat cttcagttat aaccaatctt tccaagcaac tggtaacgta
    37921 gtagggccta tgatcggctc cagtgtttcc agtgcatttg gttatcgtgg cgtctttctt
    37981 tcgacttctc ttcttgtttt ggtcaacttc ttgcttgtac gacacaatac acaagagatc
    38041 catcagcaag aaactgcaga agaaaaagct tcaggagtct ccatagcttc agggcactct
    38101 ccttctcata gacaataagc gtaaatgaaa aacactgctt gaacagttgt cacagactgt
    38161 ccttgcagtg tttttgttta ggcctgtttt ttcaagaact cagataagag gaataatccc
    38221 ttttccaagg tttctttttt acaacagtaa cctagtcggg catgcttcgg taaatcaaag
    38281 gcacttcctg gtactaatag tactcctgtt tcttctaata aattgataca aaatgtctgg
    38341 tcatcttctt cgatggctaa tttaatgaaa gaagtagaga cgtaattggg agcgacaagt
    38401 tcaactttcg gttcattggc tacccattgt tttaaaatca caaggttctc tgtaatgatc
    38461 tttttgtttc gcgccaatat tttttcttta ttcttcaatg catgtacagc aagatcatct
    38521 gacaggactc cgccgcaaat catcgtgtag tctcgatact ttcgaaaaac ttctgccaac
    38581 tcaggtccag tagctgtcca gccaatacga atacctggaa tggagtaggt tttggatagg
    38641 gaattggttg ctataccttt ttcatagaca tctacgatcg acaagaattc acctttatcc
    38701 gttaaaggcg catatacttc atctatcaat acatatgcat ctactgtacg agcaatttct
    38761 gcgatttttt ctaaagtttt ccgatctagc aatgtgcctg taggattgtt tgcactattc
    38821 aaacagatga tttttgtttc aggtttgact aaggcttcta gctgttctaa atcaaactgc
    38881 caatcttctt cttccttcaa atgaacaaag tcaacagcgg cacctaaaga tttggggatg
    38941 tcatataatt gctgataagt aggaagcatc gaaacaacat ggtctcctgc ttccactaac
    39001 gcgtataaag ctaaaagatt agcaccagtc gctccattcg tttgtaagat gttttccgga
    39061 tcgacggttt gataaagaga agcgacttct tgtttgaaaa catctgatcc ttcgatccag
    39121 ccatagtcca atggttcgtt tttgcgtttt tcgaaaaatt tacttacgct agtcccatct
    39181 aagccaacga gttcgtctaa tgtcaatgct tcgatcgagc tttgtgcaat atcatatacc
    39241 gctttctttt cccatttatt tagccattct tctacaccga aatttgcgat tttcatgttt
    39301 ctttccccct tatatcttga tccagcctac agcaagcaac ccgattgaaa gcagtgcggc
    39361 cacacaaatc agcccaatca ttactttttc ttttcccgta atttgatgtt tggtttcacg
    39421 acaagcgatc cagtagaagc aaaaaccagg aagataggca atactacata aaaggacata
    39481 agaaaatcct gctaaaatga tgcccatcaa ttcaaaaact acagcaatcc caccgatcac
    39541 tacttgtttt ttgattcctt cttttaaaga aaactggact tgatagatcc cgacaagtag
    39601 ataacaaatc aaaattgccg ctgtacataa agaataggca aattgatagg cataatcagt
    39661 aactaagaaa gtcaataaga atagattagt caagattgca gtaacaacta acgaataagt
    39721 tggggcattt tttttattcg tttttcccca acctttcggc aacagaccgt ctcgagccat
    39781 caacatcgtt gtttctgctg ggagcatcgt ccaagacaac caagaaccaa tgatcgaaat
    39841 aatcaagccg atattgatca gcgctgctcc ccaatttccc acgattcctt ttaaaatatt
    39901 tgccatagca ggttgtgaaa tagttgctaa ctcttcttga gataagacgc cgtatggcaa
    39961 gatcgaagca aggatataga taaacaataa acaaagcaga ccaatgatcg atgcttgctg
    40021 ggcttctgaa cgtttcttcg cgcggttggc taaaacactt gcgccttcga tccctacaaa
    40081 gacccacatc ataaccatca aacaaccttt aatctgcaac cagatcgttc cttgctgccc
    40141 tgttccattc actagattat cagatacgtt accccaaaaa tcagcagtga atatattcac
    40201 tttaaatgcg ataaatacaa atattaaaaa taggaataat ggcactaatt tacagatcgt
    40261 cacgattgta ttgatgacgc tcgctccttc aactccattg tttactaata aagtcagacc
    40321 ccaaataaaa atacttgcaa ataaaataga aggaacattt tgtccacctt tgaatatcgg
    40381 gaaaaattct cccatggcgc tcatcagcat cgttgcaaaa gcaacatttc ccagccacgc
    40441 agacaaccag taaccccaac cactgatgaa gccacctaat ttaccaaatg aagcttccgc
    40501 gtaaccgaaa ataccaccat ctagatctgg acgcttttct cctaaatgat tcagcgataa
    40561 taccaaagct aaaataccaa tcccaacgat tatccatgaa aggatcgctg gccctggagc
    40621 tgcagaacta gccagatcac ttgtgatccc aaacacaccg cttccgattg acgaactgac
    40681 aaccagagca accagtcccc atttgctaat acccgtcgat ttcttttcca tttccatcac
    40741 cctcacttat aaaattgaac aaatcacttg caaaagattc ccttctattt tgcaactgct
    40801 cttgcttaac aaaaagcaac tttattcata ttattggata ttatgaactt tttacacaag
    40861 tatttcaagt ataaaatgaa taaaaattaa ttttattgca aaaaaacagc tatgctatcc
    40921 aaaagaaaag gtgtttcttt tgttagccat agctgttttg attcatttaa tatcgcttaa
    40981 acgtattgta aaacttttgt ttcttgatat gcgtggtcga tcaaaccacc acccagacat
    41041 tccattccat cataaaatac aactgcttgt ccaggagtga ttgcacgtac aggttcgtcg
    41101 aatacgactt ctgcacgatt atcatccaat aagcgaactg tcacaggaac gtcttgctga
    41161 cgataacgga atttcgctgt acacttgaat tcttttggca ttggttcatt cgtcgtaaag
    41221 tggatttcac ttgcatccaa gcttgtggca tacaatgcag gatgatggaa cccttgcccc
    41281 acatataatg tgtttgtcgc tagatctttt cctacaacaa accagggttc ttgcgaatct
    41341 cctccgccac cgatccctag tccttgacgt tgaccaatcg tataatatat caagccagca
    41401 tgttgtcctt tgacttcacc atcaagagtg accatatttc ctttttttgc tggcagatag
    41461 ttgctcaaaa attgtttgaa gttcttttca ccaataaagc agattcccgt tgaatctttt
    41521 ttcttagcag tcgcaaggcc tgcgcgttca gcaattgccc gtacttctga tttttccatg
    41581 ccgcctagag ggaacatcgt ttttgaaagc tgttcttgtg ataattggct aaggaagtaa
    41641 gtttgatctt tattattgtc tactccacga agcatgtgga ccacgccgtt ttcatcgcga
    41701 gtgacttgcg cataatgtcc agtagctaca taatcggcgc caagatccat tgcataatcc
    41761 aagaatgctt tgaatttgat ttctttgttg cacatcacgt ctgggttagg tgtacgtcct
    41821 gcgcggtatt cggctaggaa atattcaaat acacgatccc aatattcttt ttcaaaattc
    41881 acagagtaat aaggaatccc gatttgagca gctactttcg ctacatcctt ataatcttcc
    41941 gtggccgtgc atacaccgtt ttcgtccgtg tcatcccaat ttttcatgaa tattccgatc
    42001 acgtcgtatc cttgttcctt taacaaaagt gctgttacag atgagtcgac accgccgctc
    42061 atccctacga caacgcgtgt tttgctgttg tctgtcatga tatcaccatc atttcaatta
    42121 gattctgaaa catctacgag ttgaaggtcg caacttttca gtattttaca ttatacgtca
    42181 tatttctgaa aatttcatcc cttaattatc aagtcgtaaa aatactgttc tactggacct
    42241 attcagtaca caaacatgat agtcaaccaa aaaatcactc atcttttgag cattcgatat
    42301 aaaaaattgg catactcata aaaagggcca gcctttcggc aataaggaat aattttataa
    42361 attgtgaaaa actatttttc aaaattgctt cttatcgctt aaagctgcac gtctcattta
    42421 caaccttttc tttttagatg ttatgagttg ctcagataag tgattttgtt tactctaaga
    42481 aaaaagggag gcaaaagata catgaattac tttatcagtg atatgcactt tttccatgaa
    42541 cgcttattag gaaaaaacga ctttgctccg cggctctttg caaatgtgga tgaaatgaac
    42601 caacagttga tcactagctg gaatgcagta ataaaagaaa ccgaccatgt ttatcatcta
    42661 ggcgaccttg ctatgcatcc gcaatatgaa aaagcaaatg cagaaatttt ggaactggtc
    42721 actcaactgc aaggaacaat ccacttgatc aaagggaacc atgacagtcg cgcttttttc
    42781 cgttatttag aaaaaaatga tcctaggctt actgacggaa gtcccaaatt ttatttttat
    42841 gatgtaggtg ccatcgttaa attcgaccat caacaatatt acttgactca ttatccgatg
    42901 ttgctcgggc ctaacgacac tatccgcaat cttcatggac atatccatca ttacagcgta
    42961 cctatcgaga acgatgtcaa tgtaggcgta gacgcaccgg aaagagaact cttaaaggaa
    43021 aaactaccat ttggcactcc gttatctgag caacaaatca aagaaatcta tgaagcaaag
    43081 aggaagtacg ataggtcgtg aaaaaactgc cctatatcaa gtattaaaca aataaagata
    43141 attactcata tttttcattt gagtaggctt attgccacag gaacattagg atcagaaatg
    43201 gagttgtaac aagacttctg tcacaactcc attccatact attcgtcttt ttgctcatgg
    43261 tgttctttta ccagtttttt ctgtaattct ttatcataat ttcgggcagc tgacgttaaa
    43321 aataaaacga ttccgcataa taaaatcccg attccggaaa acaagaacat tttttctacc
    43381 cctaaacgat cagctaatgg tcctgcaaat aataatccaa ttggacctgc caggttcatc
    43441 aaagagttga agttccctaa gacacgacct aaaacattcg attcataact ctgctggatc
    43501 attgccatca gcagtgtatt gaaatatggc gttgcacaac ctgctcctgc attcaagata
    43561 agaaagtaca agaacccttg actatcgttt ggaaccaatc cgcttgcacc gatcgtcagg
    43621 cccataatga tgtaggctat aataataggt ttcattcggt ctttccatgt tcctataaaa
    43681 ctgatcagtg caccgcctaa gagcattccg actgcgtaga ctacttccac tgctcccgcc
    43741 tgtccaacgg aaccaccaaa gtaatctagc gtcatcaatg gatacagact gattgctggc
    43801 atgaacaaca gcatcacaaa tgcaccattg atcgttatgt accataaccc tttattatcc
    43861 atcaattgct gcagcccaaa ttttgcatct ttaaaaatag taagcggaac ttccaatatt
    43921 tcaggagaaa cttttgggat cttcacaaaa atcaataagc cgacaccaaa aactgccccc
    43981 aatacatcta ataaaataag gtaattgaca ggaacgactg aaaacaaagc cgcacctaga
    44041 gctggcgcaa tgatgaaatt cgctgattgg accattccta actgtccatt cgtccttgtt
    44101 atgtgagaag atggaacgat tgtcggcata atcgactgga tcgttggcat ttggaatgtt
    44161 tgtgcgaccg aacgcataaa caaagacaca aaaactagcc aaatcgggaa ggattctgaa
    44221 atcgttccga ctactgctaa aataagcgcg aaaatcgcta caataatatc cgtgacaatc
    44281 aaaagagctt ttttatccca gcgatccact agcggtccta caaatgggct tagtaaaacc
    44341 atgggaatca ttcctagtaa agtagcaaaa ctcaaaattg tcgccgaacc ggtttctctt
    44401 gtcaaatacc agatgatcgc atattggacg accatactag tgattcctga caaaaactgt
    44461 cccgataaaa agaacaaata atttctcttc caattcgtta aaacgaccga ctgttcattt
    44521 tccataaaca tccctctttt ctttttcatt ttgtatagtg tttgcacaac aatcattata
    44581 cgggattctt ttttcaaaaa ataccttttt ccctaaataa caatcgtttc aatttaaatt
    44641 tctttactta gaaacaataa atcagcaaaa tattaaaaat aacaacatgt gttaaattta
    44701 aaaataaaaa agacaactaa tttgcgtcgt ccttacaaag ttagtcatct aacaataggc
    44761 cgtttcaaaa ctagttgtct ttttatatcc acagatcatt cgactttttc gaataaccca
    44821 cggctaatca tcccatccat cagaaaataa aatttatctt ggctcatttt ctgttttgga
    44881 tctttgaaaa tattgactgc tttcaatcca tttgctgtag tgatcgacat tttcaatgtt
    44941 tgaatatctt ttgatacagt gatttttaat ttgaatcctt ctttgctttg cggcgtggca
    45001 tctaatggtc ggatcaattg ataattgcct ccgtttgttt ctgtaaatcc attatcgcgc
    45061 aatgtatatc tttttgcatc aggatgcaaa cgatagctaa ttggtgcgcc cagtaccgtt
    45121 gctgttgttt caaatgccat gattttcacc tcgataaaat tagtttctct ttattataaa
    45181 agtttatcct gatttttact aggtttttca gttaagttcc ctaaaaaatg aaggcgttct
    45241 ctcagacatc tgttcatata agaatgactt cactagttat tttttgacag cacgaactaa
    45301 ttgatcaagg aaatactgga tgtcgtcttc ggtatttccc aaaccaaagc tgatacggat
    45361 ggattctttt agtatcggtg tatcttctcc atacattgct tctaatacgt gtgaaggctc
    45421 gatatttcct gctgtacaag ctgaaccagt agaaatagcg accccattaa gatcaagctt
    45481 catcagtagc aaatcatttg cttcgccaat taagcgaaga ttcaacacat gagggagttt
    45541 attcgttaaa tcaccattta cttgataagg gatttcgttt gcttctaatg tatcaagaat
    45601 caattgctgg aatcctttat atttgttgtt acgtgcttca cgttcttcag gtgacagcag
    45661 ttcaactgct tttgccatac cccagatccc tgctaaattt tctgtacctg cacgccgctt
    45721 ttcttcctgc tccccgccta aaagcaatgc agggatattt actccgtctc gtttatataa
    45781 gaaaccgacg ccttttggcc cattgatttt atgagcagag atacttaata aatcgattcc
    45841 taattcttct ggatggatcg tttggtttcc gtacgcttgt accgcatctg tatggaaata
    45901 agctggatga tccgctaaaa gttcaccgat ttctttgatt ggcatcaaat ttccgatttc
    45961 attgttcccg tacatcacag agaccaagat cgtatcttct cgtaacgctt ttttgacctc
    46021 ttcgatcgac agattccctt tttcatctac aggcagatat gtaacgtcaa acccctttgt
    46081 ctctaaatat ttcatggtat taagaacagc aggatgttcg atagctgtag tgattagatg
    46141 tctgccttct ttttgacgcg agaacgctgt ttctaagata gctgtattat ccccttctgt
    46201 tcccccactg ttaaagatga tctcgtgagg ttttgcttgc agactgtctg caatgatttg
    46261 tcttgctgtt tccaattttt catgcgcgtg gcgaccaaat ccatgtatgc ttgaaggatt
    46321 tccaaacgtg tcttgcatga ttgccgtcat cgcttcaatt acttgtggat gcatgggggt
    46381 agttgccgca tgatccagat aaatagtttt cacttcattc atcccttcca aattattgct
    46441 aggcacattc tagcataaaa tagcgaacaa accaaagaag agctcatttt tgtctagagg
    46501 acgtttcatg ggcatagaaa aaaggagctg tgacaaaagt cttgtcgcag ctccttgaag
    46561 ctgctcactt ctgtcacaac ttctttccct cttttttttg tttattcgaa ttctttttct
    46621 tccagacgga ataacggact catcggtgta ttttcatgga tgcgtttgac cgcttcaccc
    46681 attaattctg aacatgtaac gattgttaag ttttctggat gacgattttc ttctgtgaaa
    46741 acagaatcag tgatacaaat atctttgatt ggtgcattgt ctaaaatact tttcgcatga
    46801 tcggacaata atccatgtga cgcacaggca tatacttctt ttgcgccgtt ttcttttaat
    46861 acatttgctg ctcgtgcaag cgttgctcct gtgttcaaga tatcatctac catgatacat
    46921 gtctttcctt gaacatcacc gatcacataa ccaccctcag ttgaagcatc atctgcatga
    46981 tctacgatgg ctaatgtgct gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg
    47041 ctgttttttg gcgaaacaac tacgatgtca tctcccgtca gacctaattg acggtaataa
    47101 tgagcaaaca acggcatcgt aaataaatta tctacaggaa tatcaaagaa tccttgtact
    47161 tgaaccgtgt gcagatctaa agtcagtact cgagttgccc ctgcttcttc gatcaagtta
    47221 gcgatcaatt tcgcagtgat cggttcatga ggtttcgccg tgcggtcttg cctagcatac
    47281 ccatagtaag gcaatacgac attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc
    47341 atgatcagta gttccatata gtaatcattg acaggtgcat tcgttgattg aacgatatac
    47401 acatgatccc ctcgaatact ttcttcgata ttgatggaaa tctcgccatc actaaattgc
    47461 ttcacgacgc ttttacctaa ttctgttccg aacactttcg cgattttttc agctaatgga
    47521 cggttgccat tcaaactaaa aatcttcaac gtgtcatcct gatacttatt actcatgtag
    47581 tcctccccaa aacttctcta ttcttcgata gtttaacata tttttccaag attttctgtt
    47641 aatttcagca aattcttaag gaagtgaaac tgttccgtta ctccaactgg caggaatgac
    47701 tttatgataa tcgccccagt tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc
    47761 aaaataagcg tacgctgtca cccaatgatt gccatatgta ctgccaagta ctttcaaaat
    47821 accaatcatc acaggtttgc cttcacgtat acgttttgta gctcgctgcc atgaacctag
    47881 aagagttgct cgtgcacgct catgattgcc attcttcttt aaaaaactag aaatccctgt
    47941 actaacctga aaaggaatcg taggaagtcc tagtggttgg atctggctac gcaatgcctt
    48001 cgtcaaaacc aacgaatcag ccgtattttt cttgcgaatc tcatttggaa gcatccaccc
    48061 cttacgatag tcttgataat aggcaaggag aactgctgcc gcgtaagtac cacaaagata
    48121 gcctgagggg gtagcccatt tcttatacct caaaaaacgt tcttctggca atcccaccca
    48181 gcaatttgcg agttcctttc ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt
    48241 tctgttgatc catctagata actcgattgg ctcttcattg atcagcttcc attttttaca
    48301 agaaagaccc tcttgataag tatattttgc tggagaatat tctaccggat gtcctgattg
    48361 atcgataatc acataacttt gatcttcaaa aaccagagct aaaaaaagca atttactttt
    48421 tcttgactgc agctgctgag tgctggctaa ttttttaatt tttttatcta
//
LOCUS       DACDIP010000014        47470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDIP010000014 DACDIP010000000
VERSION     DACDIP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.014"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IV257_000973"
     CDS             complement(53..787)
                     /locus_tag="IV257_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8814120.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IV257_000974"
     CDS             1114..1524
                     /locus_tag="IV257_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8814121.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IV257_000975"
     CDS             1517..2218
                     /locus_tag="IV257_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8814122.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IV257_000976"
     CDS             2218..3804
                     /locus_tag="IV257_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8814123.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IV257_000977"
     CDS             3823..4044
                     /locus_tag="IV257_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8814124.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IV257_000978"
     CDS             4041..4802
                     /locus_tag="IV257_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8814125.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IV257_000979"
     CDS             5182..5691
                     /locus_tag="IV257_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8814126.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IV257_000980"
     CDS             complement(5766..6305)
                     /locus_tag="IV257_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8814127.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IV257_000981"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IV257_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8814128.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IV257_000982"
     CDS             7372..8169
                     /locus_tag="IV257_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8814129.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IV257_000983"
     CDS             8197..8832
                     /locus_tag="IV257_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8814130.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IV257_000984"
     CDS             8852..9625
                     /locus_tag="IV257_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8814131.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IV257_000985"
     CDS             10033..10830
                     /locus_tag="IV257_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8814132.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IV257_000986"
     CDS             10808..11221
                     /locus_tag="IV257_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814133.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IV257_000987"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IV257_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8814134.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IV257_000988"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IV257_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8814135.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IV257_000989"
     CDS             15998..16558
                     /locus_tag="IV257_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8814136.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IV257_000990"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IV257_000990"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8814137.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IV257_000991"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IV257_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8814138.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IV257_000992"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IV257_000992"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8814139.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IV257_000993"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IV257_000993"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8814140.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IV257_000994"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IV257_000994"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8814141.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IV257_000995"
     CDS             21905..22483
                     /locus_tag="IV257_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8814142.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IV257_000996"
     CDS             22535..23017
                     /locus_tag="IV257_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8814143.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IV257_000997"
     CDS             23134..23508
                     /locus_tag="IV257_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814144.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="IV257_000998"
     CDS             complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="IV257_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8814145.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25685..26275
                     /locus_tag="IV257_000999"
     CDS             25685..26275
                     /locus_tag="IV257_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8814146.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26403..27752)
                     /locus_tag="IV257_001000"
     CDS             complement(26403..27752)
                     /locus_tag="IV257_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8814147.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27920..28660)
                     /locus_tag="IV257_001001"
     CDS             complement(27920..28660)
                     /locus_tag="IV257_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8814148.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28673..30265)
                     /locus_tag="IV257_001002"
     CDS             complement(28673..30265)
                     /locus_tag="IV257_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8814149.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30833..31129)
                     /locus_tag="IV257_001003"
     CDS             complement(30833..31129)
                     /locus_tag="IV257_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814150.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31305..32030
                     /locus_tag="IV257_001004"
     CDS             31305..32030
                     /locus_tag="IV257_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8814151.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32023..32865
                     /locus_tag="IV257_001005"
     CDS             32023..32865
                     /locus_tag="IV257_001005"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8814152.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32868..33389
                     /locus_tag="IV257_001006"
     CDS             32868..33389
                     /locus_tag="IV257_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8814153.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33642..34205)
                     /locus_tag="IV257_001007"
     CDS             complement(33642..34205)
                     /locus_tag="IV257_001007"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8814154.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34456..34725
                     /gene="rpsO"
                     /locus_tag="IV257_001008"
     CDS             34456..34725
                     /gene="rpsO"
                     /locus_tag="IV257_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8814155.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34913..37039
                     /gene="pnp"
                     /locus_tag="IV257_001009"
     CDS             34913..37039
                     /gene="pnp"
                     /locus_tag="IV257_001009"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8814156.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37549..37815
                     /locus_tag="IV257_001010"
     CDS             37549..37815
                     /locus_tag="IV257_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8814157.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37959..39950
                     /locus_tag="IV257_001011"
     CDS             37959..39950
                     /locus_tag="IV257_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8814158.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40304..41605
                     /locus_tag="IV257_001012"
     CDS             40304..41605
                     /locus_tag="IV257_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8814159.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41735..43105)
                     /locus_tag="IV257_001013"
     CDS             complement(41735..43105)
                     /locus_tag="IV257_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8814160.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43287..43610)
                     /locus_tag="IV257_001014"
     CDS             complement(43287..43610)
                     /locus_tag="IV257_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8814161.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43935..44543)
                     /locus_tag="IV257_001015"
     CDS             complement(43935..44543)
                     /locus_tag="IV257_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8814162.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44873..45589
                     /locus_tag="IV257_001016"
     CDS             44873..45589
                     /locus_tag="IV257_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8814163.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45602..46297
                     /locus_tag="IV257_001017"
     CDS             45602..46297
                     /locus_tag="IV257_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8814164.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46381..47010
                     /gene="udk"
                     /locus_tag="IV257_001018"
     CDS             46381..47010
                     /gene="udk"
                     /locus_tag="IV257_001018"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8814165.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15391 a         8788 c         9878 g        13413 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcacagcatg acagcattgg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ctagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt ttgcgaaaac tatttttata tcatatatat gtctaaaatc tatattataa
    23881 aagaaatgtt gtttgatcag cttctcttcg gtattagttc aaaacaaaac ggcaaagtat
    23941 atggagctac tttgccgttt tgtttctcta atactcaggt cagtttatag acgctcaata
    24001 aacggtgttc aaaagccagt ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag
    24061 ccattttcaa aaaatatttc ttaatactcg aaactgagcg gctttttcac gacctctcaa
    24121 taaacagtgt tcaagagaca gcctatcggc aataaggctg aacgtctctt tcacaacctc
    24181 ttttatcaat ctgctacttc ttctttctta gcgaaactcc aaatcagtcc gataacaaaa
    24241 ccgataactg ctggtacgat ccaccccatc cccaatttga aaaacggcaa gtagctttcc
    24301 gcaacatata aaagattttg aacaataggt gtttgtttga tagtttctgg gcaagcgttc
    24361 aacccatcaa aaatcgaagc aatcaatgta aaataagtag tcattcgata aacagccgta
    24421 cgctgcttaa acaaaggacc aactaacact agtaaaatca gtgtcattgc taatggatag
    24481 atgaacatca aaacaggcaa cgaaaattga atgatattcg tcaagcctac gtttgccgct
    24541 aaacaaggaa ggatactagc aagtgcaaca aagaatgcgt aatttctttg cggaaacaaa
    24601 tctgtgaatg tttctgcaaa agctgtaatc aaaccaatac cggtcttcaa gcaagctaat
    24661 ataacgataa atgctaataa gatactgcca taagtaccta aataatgatc agcaatctga
    24721 gccagcgtga tcccgccatt ttcacttaga gcaaagctac ctaagctcat agcacctaaa
    24781 taagcgagca aagtataaat gattcccatc aatataatgc tgatcgcacc agatttgatc
    24841 gtgtctttag cgatttcagc aggttttgtt acacccatgt tttggattgt tgtgacaatg
    24901 atgatcccaa atgctaaagc agcaagcgca tctaatgtgt tatatccttg agtaaaccct
    24961 gtgaaaaaag catgctcttg ataatttggc tgaataggcg cttgatttac agaacctagt
    25021 ggattcaaaa aagcaaggag aagcaaagca cctaataaaa ccaaaaatgc aggattcagg
    25081 aactttccaa cataatctaa tatctttgtc ggttttcttg aaaaccacca agccagcaca
    25141 aaaaatacaa tactgaaaat tgctagatat agcgtctgtc tgctaggatc gataaatggt
    25201 gctaaaccaa tctcgaaaga tgtggttgca aggcgaggta aagcaaaaaa cggtccgata
    25261 actaagtaaa gcaagatggt aaaaaccatc gcatatgttt gatttatccg ttttgctaat
    25321 tcatatacac ctgagctttt cgatacacca atggcaatca cccctaagaa gggaagaccg
    25381 ataccggtaa ccaagaaacc tagattcgcc caaaaggtag cagcgcctgc ttcttgaccc
    25441 aaatgaacgg gaaagataag atttccagca ccaaaaaata gaccgaaaag catggagcct
    25501 ataaaaagat agtctttaaa tgataatttt tttgtcatac ataactcctt tacggttttt
    25561 ttaaaagtaa caaaatttcg aaaaatatgc aacaattttc agaaaatttc ctatataaaa
    25621 cgacggttat ttaataacgt aagtgataaa ataaaagcgc tgaatcatct gaggaggaat
    25681 catcgtgaca aagtcgagat tagaagcatt tactgatgga gtggtagcca ttgttttgag
    25741 agttcttgta ttagagatca agatccctaa tacatctagc tttcattctt tatgggtcat
    25801 ccgaaataca ttattagctt ataccatcag ctttatattt gttgcggtga tctgggtgaa
    25861 ccatcatcgt atatttcaga tggcagaacg aattaattac cgagtggtct ggtcgaatat
    25921 ctttttgctc ttttggctga ctttatgtcc ttctgttact agttgggtcg ggcgtaaccc
    25981 tgaagctttt tggcctgaga tggcttatgt cttagtatac acgatgtgga gtttttcttt
    26041 tggtatcttg atgaggcaaa tcatgaaagc aaataaacca gattcccatg tcgtgagagt
    26101 tttgtcaaag gaccgtcgaa gtcttatctc catgatgatt aacttatgct tgattgccgg
    26161 tgtctttttt gtaccgttga tcgggttatt cggacgtttt tttgtatctg gtatttggat
    26221 tttttcctat aaaaaagcag atgcctatta tcagcggttg tttccgggga aatagaaaag
    26281 aggctgggac agaagctttt aactccaaga aataagccga aatttcacaa aaatttaaga
    26341 agcaattttc gtaaattcct tcttatttct cgaagctgac cacttctgtc acaactactt
    26401 tcttacttct tacgttcata tttcatatga tagattagta atagaagcaa gaaccacaca
    26461 ggtgttagta ataaagcgat tctcgtatct tttcctagga acaagacgac acacacaaag
    26521 gccatgtaaa gaaataccag ataattacta aatgggtaca acggcatttt aaatgaatga
    26581 gcttttttgc cgaggatact tttacgatat ttcatgtgcg tgtagacaag catactccag
    26641 ataaacaaga aacaagtagt ggcaatactg cttactagtg tgaatacttc actaggcatc
    26701 acgtaattca agataacaga aatgaaaatg acgatagtag aaaatgtcaa agcattccct
    26761 ggcacatgat gtgttgttaa ctttttgaat tttttagggg cactgccttc ttgcgctaaa
    26821 gaacgaagca tacgtcctgt actatatatt gcactgttgc aggcagaagc tgctgcactt
    26881 aatacgacta aattgatcaa ggaagcagca atcgtgatcc caatttccga aaatacttct
    26941 acaaatggac tgttatctgg attcaagctg ttccatggat agattgccat aatgacaaaa
    27001 agagatccta agtaaaataa gatgatcctg ataggaatat tattgatagc ttccggcaac
    27061 actttttcag gattttctgt ttctccagcg actaatccaa ctaattctat cccagcaaaa
    27121 gcaaaggtta ccatttggaa agctaagata aatccttttg caccagttgg aaagaatccg
    27181 ccatgagacc atagattcgt gatactggct gtgccaatat ctgtcgtata gtgggtgagg
    27241 atcatataag ctcctacgag gataaaggca atgatcgcta caactttgat caatgcaaac
    27301 caaaactcta actcaccaaa caatgagaca gccaccatat tcaagcaaag aagaagacct
    27361 aatgcaatca gttcaggaag ccactgagga acaccaggcg cccagtagcg tacatacatt
    27421 ccgatggctg ttaaatcggc cattgcaatc gatacccagc agaaccaata cgtccagcca
    27481 gtgatgaaag ccgctttttt gccaagatat tctgcaacaa aatccagaaa agaatggtta
    27541 ttggtgtttg ataacagaag ttcgcctaac gcacgcataa tgaaaaaaca gatgcatcca
    27601 gtaataagat aggcgagcaa tatcgaaggg ccagctagct ggatagactt tccggaacct
    27661 aaaaacaacc ccgttccaat cgctccgcca atagaaatca gttggacatg tctatttttc
    27721 agtccacgct gcagttcttg ctcttcattc atttttaaat catcctaact aaaaaagttt
    27781 aattacttcc atattaaaag agttttctga aaaacacaac taagattcca aaattttcag
    27841 aaatttacct tttatgagta attcataaaa aagaactgcg gtaaacaccg cagctctttg
    27901 tcccaatcag ctattgatcc taagcattta aaatacgtga aaggaattct tttgtacgtt
    27961 cttctttcgg atggacaaag atatcttctg gtgtaccttc ttctgcaatc acacctttat
    28021 ccataaaaat gacacggtca gatacatctt ttgcaaattc catctcatgc gtcacgataa
    28081 gcattgtcaa gccagtatgt gctaagtctt tgatcgtatt aagcacttcg ccaaccattt
    28141 cagggtctaa cgccgaagta ggttcgtcga atagcaatac atctggattc attgataatg
    28201 cccgagcaat tgcgacacgc tgtttctgtc caccagataa ttgtgctggt ttggcttcga
    28261 tataacgatc cataccaact ttttccagat tttcgattgc tacttttttg gcagtttctt
    28321 tatctcgttt caggacagtt gtttgaccag acatacagtt ttccaagaca ttcatattgt
    28381 tgaacaggtt gaaggattgg aaaaccatac ctagattcgt tcgatattta ggtaaattat
    28441 atttaggagc taatacattt tctccattat aaatgatctc gccgcctgtt ggtttttcca
    28501 gtaaattgat acagcgcaag aaagtagatt taccagaacc ggaagaaccg atgatcgtca
    28561 caacttcacc tttattgact gttacgctga tatctttcaa tacttcattt tcaccgaagc
    28621 tttttcttag atgtttgatt tcaataattt tttcattcat gattcttcct ccttattcct
    28681 gcaaatttga attgtcgatt tcttcaagct tggtataagc tgaaggacca tcgattttct
    28741 tttctaccgc ccgcaggatt ctggtgatcg taaaggtcat gactaagtag atgatcccga
    28801 cgatcgtaaa tgtttggaag aattggaaat tcgcaccgga agcagagttt ccttggaaga
    28861 acaaatcagc tacgccgatt acacttaaaa ctgctgtatc tttgatattg ataacaaact
    28921 catttcctgt tgctggcaag atattccgta aaacttgtgg aataacaact tttctcatcg
    28981 tttggccatg ggtcatacca atggcttgcg cagcttcgaa ctgcccttca tctactgcaa
    29041 aaatcccccc acgaacgatc tctgtcatgt aagctccagt attgagagag acaataaaca
    29101 aagcggcaat cgttcggttc aatgaaatgc caaaagcgag tgccagaccg tagaagatga
    29161 ccattgcttg taccatcatc ggtgttccgc ggaatacttc gacatagata gaaagaagtg
    29221 catttccgat tttttggaag aagcgagtca gtgcatttcc tgattcaggt accgaacgga
    29281 agacaccaat cagtaatcca agtgtagtac cgacaactgt cccgatcaat gcgatgaaca
    29341 aggtaagtcc agcacctcgt aagaacatat tgccgtattg tttccaaatc gtagtgaaat
    29401 cagaaatgat gccaggtttt tcttcactat cagttgcagc aggctgatct ttgactgctt
    29461 ggtccataat tttggttcgt tcatcgttag agatgccaga aagaatctgg tttacttttt
    29521 ggatatccgg gtcgccttta cgcataccaa cagaaatctg aacatcttcc gggtttgtct
    29581 ggaagccatc tgcaccgctg aattcaagca tttccaagtc tttgttcaca ctagttgccg
    29641 ttacaccttc cggacgttcg ctgacataac catcgatcat tccagaagct aaagctgttc
    29701 tcattgctga gaaattatcc attgcttgtt gtttctgcac atctggtatc tgatcgatca
    29761 cagtatagtg gaatgtattt aactgagcag taatctttgc tccagaaaga tctgctaaac
    29821 tttttgcttt tgcatatttg ctatcttttt gaactactac tactaattgt gattcgtaat
    29881 aaggatcggt aaaatcaatt tcttttcgtc gttcagcagt agggctcatt ccggcgatga
    29941 tcgcgtcgat cttgcctgat tgaagggcag gagccagacc atcccatttc gtttggacga
    30001 tgactaattt tttccctaaa ccatctgcta cttttttagc gatttgaaca tcataaccgc
    30061 cggcgtaact attttggcct tgaattggca aggcattatt ctcatctgtt tgttgagacc
    30121 agttgaaggg tgcataccca gcctccatac ctacgcgaaa ctcatcgctc ggtgtttttt
    30181 catctgcatt tgccagagat cccacactga atagtgagaa gacaaaagca aatagtacgg
    30241 caactgaaac catatttttt ttcatttttt tctccctttc tgattatatg atacacgagc
    30301 ggtctagatg aaatgcggta aagctttatc tgaaaaaata aaaagcgacc gatttataga
    30361 tataaaaagg ccgctaaaat tccataaggt gataaacctc tcacaaaaca tagcgcaact
    30421 tagcatcact gctaagacag tccgcaagct cttaacttgc gtcccaacat acactccaag
    30481 caaaaaagtg cacatttcgg cgatcctccc tattcctgct tctccatgtt tgcttcactc
    30541 cacagggtta atgattctcg cgacctctac ctcattgatt gaggcatctc gtattcagtt
    30601 atttatacaa aaaaagttta cgatactcga gaggctttgt caatctataa ctgctgtttt
    30661 ttgaaaaaat actgataaat atagaaatct ttacttttac cgaaaaaaca atcaaaatgt
    30721 gaatcatcta tccattgcaa acgatagtat aattatacac aaaaatgtat gtaatacaat
    30781 aaaaaaagaa ctagaacaga aaatttcctg tgtccagttc catctcgatc atttaagctg
    30841 ccgctttctt tggctcacgt cctaggatcg cttgcaaaag aacaacgacg ccataaatgg
    30901 ctaatattag ataaatcaaa cttgagtatt gggtgattcc ccagccccat gttcgatata
    30961 cggctaatgt aatggctaaa acaattgcgg caatcacgtt gaataaagca aaagcccaaa
    31021 gatttccatt ttctttgcgt acaagataaa aaacagagaa ataaacgctt aacgctaacc
    31081 aggcgataca agccagaata acgccccaat aaataaagaa tggtaacata tcgattcacc
    31141 tcacttttat acagcagtat tgtaacacgt tttcattctt ttgtcagtga acattgtgat
    31201 aaattgttca aatgcccata acactttttt attcccattt ttctgacttt tcaacaataa
    31261 atttgttcga gtatgggcta taatggaaag tgaggtgaca aaacatgaaa caattaacag
    31321 tagacgaagc agcaaaagag atattagctt atcagcaaat tacatttttg accggtgcag
    31381 gggtatccac accttctgga gtgccagact atcgatcttt gtcaggaatc taccacggga
    31441 tggagcagcc ggaatacttg ctgagtcatc aagcaatggt gaatgaaccg gataaattct
    31501 atcaatttgt caaaaagatt tatcatcctg aagcaaaacc caatatcatt catttggaaa
    31561 tggcacatct agcgaaagaa aaaaatgttt gggttgtttc ccaaaacatc gacggtttgc
    31621 acgcaaaggc taaaagcccg caactcgtca attttcatgg aagtttgtat caatgtcatt
    31681 gtcgaaaatg tcaacaaacg gtagactgga aagaatatct tcacagcgac aaacatcaag
    31741 catgcggagg acaaatacgc ccagacatcg tgttatatgg tgaagggttt caagatggag
    31801 tcatggagca ggcggcttat gccgtcagcc aagcagagct gatcgtcatt gttggaacga
    31861 gttttcaagt tcatccgttt tgtgatctgg tacagtttcg ccagccgtca gccaaaatct
    31921 tagtaatcaa tcaaacgcct gtttatttag cggagcctta tacgtttatt caagaagacg
    31981 gagcggttgt tttcaaaaaa atacaagaat taggagcaga ttatgactga attcaaaacc
    32041 gaaaaagaaa aaatgattgc aggcgagctt tattttgcca atgatcctga gctggttgcc
    32101 gaccgtatgt ttgcaagaag tcaaagccaa atcatcaacc aagcagaatc agcggaactg
    32161 cgatcccaat tgttgaaaga gacttttggc cgtacaggga aaaaaatcta tatggagcca
    32221 gttatcaatt ttgattacgg atataatata tttgttgggg agaattttta tgcaaatttc
    32281 aattgtacat ttttagatgt cagcacgatt gaaatcggtg ataactgcat gttcgctccg
    32341 aatgtccagc tttacacggc gacgcatcca ctacatccag tgaaacgtaa cagcggtctg
    32401 gagtacgcaa aacccattaa aatcggtaat aatgtttggt taggcggagg agttatcgtc
    32461 acgccgggtg tcactctagg ggacaacgta gtagtcggcg caggttcggt tgtgaccaaa
    32521 tcttttcccg acaatgtagt gattgcagga aatcctgctc gtatcatcaa gacagtagag
    32581 gaagaactaa cagaagagtc tcttgaaaca ttacgtcata agatcgatat gatcgatcga
    32641 gagttgattg ctttattaga aaaaagaatg gatacggtaa ctaaaatagg acaagtgaaa
    32701 aaagaagaaa accaagcagt ttatgatgca aaaagagagc agcaagtatt ggataaagtt
    32761 gttagtctgc tgaaaaataa gggatataag gaaacaataa cagatacgta tgttgatttg
    32821 atgaaacact cgcgcgaata ccaaaacaaa atgaaggagg aataagtgtg atcgaaggag
    32881 agacacggcg aaaacaaatc atgcaaacct tggcaaatac agaaaaacca gtgagtgcta
    32941 gcaagtttgc gaaaactttt ggagtcagcc gtcagatcat tgtaggcgat attgctttac
    33001 tacgagctgc aggtaaaagt atcgtggcaa cagcaagagg ttatatcctc gaaacagaag
    33061 aaaccaatac tgggcacatc agtaaaatcg ctgtccagca tggaaaagat caaacagaag
    33121 aagaactgag actaatcgtt gaaaatggtg gtgaaatcat tgatgtaatt gtggaacatc
    33181 ccctttacgg tgagttagca ggaactttgc acatcaaaac gccacaagat atcgattcct
    33241 ttatgagacg ttatcaaaaa agtaatgcta ctttgttatc cgaattaaca ggcggcatcc
    33301 atctgcatac gatccgctac ccagaaaagc atatgctgaa aaacatcaaa caaaaactag
    33361 ctgaagcagg aatcctctac gaaggataac atcgtagctg ctacttttta tcctagaaac
    33421 tattctatca tctctgagat aaacacccat atggaagaaa taagaaaaaa taagaaaaga
    33481 tgcgtatcca atacacagaa ttttttggaa ttgcgatttc tagatgtcta tctaaatttc
    33541 gattagtgga gaaacataaa aaagggaagt gcagcttttg agcactcccc ttttttataa
    33601 agctttcttg tagcatagta tcgaaggaac ctgccaattc tctactcgat caccaatacg
    33661 ccttctttta gtgcaaatgg attttgatcg ttgatgtggt catagaacat cacaccgttg
    33721 atatggtcga tttcatgttg aacgacgatc gattcataat tcttcaaacg aatcttgtgt
    33781 ttttcaccat tcatatcata ataagacaca gtgattttgg catggcgaac aacatatcct
    33841 ggtacttctc gatcaacaga taaacagcct tcaccttccc ctaaacaagc atcttgaaca
    33901 gaatggctca aaattttagg attatacata acagtactta gagaaggtcc atctgcctct
    33961 gggtcaggac ttggtacgtg aacagcaatg attcttttag aaatatctaa ttgaggagca
    34021 gctaagccaa caccgcctcg taagtgtagt tcttcagctt tgattggatc ttggctgttt
    34081 tttaagaact caagcatctc ttttcctaaa gagatatctt cttcgcttaa tggaagagaa
    34141 acttcttttg caacttcgcg taaggtcgga tttccttcac gaatgatatc atccattgta
    34201 atcatggaat atgtacccct ttcttaaaag actgtgtttc aagtctattt tcgcattata
    34261 gtttaccaca aaaatggaaa agtagtagta agattgtttg aaaactacct tttcttaatg
    34321 attaactact tgattttcaa cccggtttca ggtaaagtat agctgtgtgc tctaggcaca
    34381 tgacctgcca cttggttaag cgaatcacca acgttttact cagtcgcagg ggaataatcg
    34441 aaagaggtga aagaaatggc aatttcaaaa gaacgcaaga atgaaatcat caacgaatac
    34501 gctcgccatg aaggggatac tggttctcca gaagtacaaa tcgctgtatt aactgaagaa
    34561 atcaaccact tgaatgaaca tgcacgcgtg cacaaaaaag accatcattc ataccgcggc
    34621 ctaatgaaaa aagttggtca ccgccgtaac ttgttagctt acttacgtaa aactgacgtt
    34681 caacgttacc gcgaattgat caaacgttta ggcttgcgtc gttaatttta gtcaaaaaga
    34741 gtgggattca catgaatctc acttttttta ctcgtatatc aattaagtaa aattccgtga
    34801 tcaagctaga tggactccta ctaaccaaat gaaagctgct caaaagacgg ctttcggcag
    34861 ttttcatttg tttagtagcg tcttgctttc acgagaagga aaaggagaag ttatgtcaga
    34921 aaaacaagta ttcaaaacga catggggcgg ccgtccctta caaatcgaag tcggtcagct
    34981 agcaaaacaa gcaaacggag ccgttttagt ccgttatgga gatacagtag tcttaagtgc
    35041 agctgtggcc tctaaagaag caaaagatac agactttttc ccattaacga tcaattatga
    35101 agaaaaaatg tatgcagcag ggaaaatccc tggaggtttc atcaaacgag aaggccgtcc
    35161 aagtacggaa gcaacattga ctgcccgttt gatcgaccgt ccaattcgcc cgatgtttgc
    35221 agaaggcttc cgtaatgaag tccaagtaac gaacatcgtg atgagtgtag aaacagactg
    35281 ctcgccagct atggcagcaa tgctaggttc ttctttagca ctatcaatct cagatatccc
    35341 atttgacggc ccaatcgcag gcgtagaagt tggtcgtgta aatggcgagt acgtcttgaa
    35401 tccaacagta gaacaagcag aacaaacaga tatcgaattg actgttgccg gaacgaaaca
    35461 agccatcaat atggttgaaa gcggcgcgaa agaagtttca gaagaagaca tgcttggtgc
    35521 attgctgttt ggttttgacg ctatcaaaga gttagttgct ttccaagaag aaatcgttca
    35581 agcagtaggg aaagaaaaaa tggaagtgac tttactgcaa gttgatgaag tattgaaaaa
    35641 agaaatcttt gatgcttctt acgctacaat gaaagcagca gtaatgactg aagaaaaact
    35701 tgctcgcgaa gataatatcg agcaggtgaa gatcgatatc cgcgaagctt atgctgagaa
    35761 atttgcaggt catgaagatg aagatcactt gttgaaagaa gttaaacaaa tcacagaaga
    35821 tcttgaaaaa gatgtcgtac gtgaattgat caccatcgat aagatccgtc ctgacgggcg
    35881 taaattggat gaaatccgtc cattgtcatc agaggtcagc ttattgccac gtgtacatgg
    35941 atcaggtttg ttcacacgag gacaaacaca agctttatca gcttgtacac tcgcacctct
    36001 aggagagcac caaatcattg atggtttagg ggtggaagtc agcaaacgat tcatccacca
    36061 ctacaatttc cctcaatttt ctgttggttc tacaggacgt gcaggctctc cagggcgtcg
    36121 ggaaatcgga catggcgcat taggtgaacg tgcgttagca caagtaatcc cttcagaaga
    36181 agaatttcct tatacgatcc gtttagtagc agaagtattg gaatcaaatg gttcttcttc
    36241 ccaagcaagt atctgtgctg gcacattagc attgatggat gcaggtgtac caatcaaagc
    36301 gccagtcgcc ggtattgcga tgggacttgt ttctgatggt gaaaactata cgatcctaac
    36361 tgatatccaa gggctggaag atcatcttgg cgatatggac tttaaagttg ccggtacaaa
    36421 agatggtatc actgctttac agatggatat caagattcaa ggaatcacag aacaaatcct
    36481 aactgaagca ttaactcaag caaaacaagc acgtatggaa atcttagaag aattgacttc
    36541 aacgattgct gctccacgtg aagaattaag ccaatatgca ccaaaaatcg aaatgatcca
    36601 gatcgaacct gctaaaatca aagatgttat cggtaaaggc ggcgatacga tcaacggaat
    36661 catcgatgaa actggtgtga aaatcgatat cgatcaagat ggaaaagtaa gtattgcttc
    36721 agctgatgca gaaatgatca aaaaagctat caagatcatc gaagatttga ccaaagaagt
    36781 caaagttggt gaagtttatc tagggaaagt tgtccgtatc gaaaaattcg gtgcattcgt
    36841 caacttgatc aaaggaaaag atggacttgt ccatatttcc caattagcaa acgatcgtgt
    36901 caataaagtc gaagatgttg ttaaactagg tgatgagatc cttgtgaaag taactgaaat
    36961 cgataaacaa ggacgcgtaa atctgtcaag aaaagcaatg ttgaacgaag atggttcaac
    37021 gaaaaatgaa gaaaaataat ttaattcata ggaataatgt acttgtgatc gtgaaggtga
    37081 cagggggaca ataattattc gaaaaaaggg gactgtgaca aaagccgtgt cacagtcctt
    37141 ttttttcgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc agccaagtta
    37201 aaaacattag gagctgtttt tatttggctg ttcttattac ttgatgcaga acagcttttt
    37261 tacaacctct ccaataaacg atgttcaatc agctgtccct cgatattagc tcaaaaaacg
    37321 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggtca aaccgctgat
    37381 ttcacaacct ctgattcacg gtgttccaga agcagcttct acggaaataa gctgaacact
    37441 tttgttacaa cttctttttt tctggagttt cttggcgcat atttttggag tccttctgaa
    37501 taaagcgtaa aataaaagtg agaaatatgg ataaaggagg gaaaggagat gctgaatttt
    37561 ttatggtcgt tatttatcgg cggtatatta ggtttgatag ctggcgcaat tttaggaaag
    37621 gaagtcccgg gaggcgtatc tggaaatatc attatcggtt ttttaggtag ttggctcggc
    37681 gaatttttac taggaccact gggaccggaa ctcggcggtt tttatttgat accagcatta
    37741 ttgggtgcaa ttttttgcct tgctctttat tcatttgtgg caagacgtat cagagaacgt
    37801 ccaaagaaag aataatttta ttcaaataag ttgacacttc agtgtgttac ttggataatt
    37861 agaagcaatt taggaaagcg ggtgtcatgg ataagaatat catctatcat tcaagcttac
    37921 ctaaaggata tccatataac aagaaatgag ggaaaaacgt ggcaacaacg cacaatacca
    37981 aaaagatatc aatgactggc ctgcttgtag cgatgggagt cgtctacgga gacatcggga
    38041 caagtccgct ttacgttatg aaatcgattg ttgaagataa tggcggattg tcaggaatca
    38101 atcctgattt tgttatcgga tcagtctcat tgattttttg gacattgact ctactgacaa
    38161 caatcaaata tgtcgtgatt gcattgaatg cagataacca tggtgaaggt ggaattttct
    38221 cactctacac acttgtccga aaaggtggga aatatttgat cattcctgca atgatcggag
    38281 gagctgcctt actagcagat ggggtattga cccctgctgt gacggtaacg actgcgattg
    38341 aaggcttgcg aggaattccg cagttttttg accggttcgg taataaccag aacgtgatcg
    38401 tcattatcac tttagttatt atttttgttt tattcatggt ccaacgcttt ggtacagata
    38461 ttgtcggaaa agcattcgga ccaatcatgt tcgcttggtt tactttttta ggcgtaatgg
    38521 gattaatgaa cttcagtcaa gactggacag ttatccgtgc actgaatcct tattatgcta
    38581 tccagctgct ttttagtgca gataacaaat taggcttgtt cattcttgga aatattttcc
    38641 tggctacgac aggtgcagag gcgctttatt ccgatcttgg acatgtcggc aaacgaaata
    38701 ttcgtctgag ctggccttac gtcaaaattt gtttggttat caattatttt gggcaggctg
    38761 cgtggttgtt gaatgtttat caaaaccctg aagcacagca aatcaagaac ctgaatccat
    38821 tcttccaaat gatgcctcaa agttggatcg tgatcggcgt tgcttttgca acagtcgctg
    38881 cagtcattgc ttcgcaagca ttgattacag gttcgtttac gctagtttca gaagcaatca
    38941 agttgaaact gctgcctcgg ttaaagatca tgtatcctgg gaacagtatc gggcaaatgt
    39001 atatcccagc agtcaatctg attttatgga tcgcctgttc tctagtggtt gtcacattcc
    39061 gaacgtctca tcatatggaa gctgcttacg gtctatcgat cactgtgacg atgttgatga
    39121 caacagcact gctttacttt tatcttatcc aaaatgggca ttctcgttgg cttgcatatt
    39181 tagttacctt tttcttcggt gcgatagaag tcgttttctt tatctcaagt gttgtcaaat
    39241 tcttccgagg cggttttgta gccgttctga ttggtttgat catcttagtc gtgatgttta
    39301 tttgggaaca agggaacatt attcgcgaat cgtcaaccga ggatgtgtct ttgagagatt
    39361 atatcccgca gcttagagaa ttacgtgatg atacttcttt accaatctat cagacaaatg
    39421 tggtcttctt ggttcctgat atggctgaag ataaagttgg ccgacagttc atctattcga
    39481 ttttagataa acggcctaaa agagcacgag tctattggtt tgttcatgtg gaagtgacgg
    39541 atgagccata tacgaaagaa tatcaagtag atatgatgga cactgatttt attgtccaag
    39601 tgaatctgtt cttaggtttc agggtccaac aggaaatcaa cgtatatgtt cgccaagtca
    39661 tccatgattt gatgaaacaa ggccgtttgc caaaacagcc acaaagatac tctctgacac
    39721 cagggcgaga agttggcgat ttccaatttg tcctgattga tgaagtagtt tccaatgtga
    39781 caactctagg aaaatggcaa cgtcaaatca tgcaggcaaa actggcaatc aaaaaaatcg
    39841 ctacctcgcc agaaacatgg tttgggctag agtacagcga agtaaaacat gaaactgttc
    39901 cgcttatcat tggaaatact cggaaaacat ggttgaaaga gcggttgtga aagtagcgct
    39961 ttgacctgaa ctaatgtcga atgtcagcta cttgaacacc gtttatcaga ggtcatgaaa
    40021 aagctgtact gcaacaagca gtaagaacga gcagataaaa acagcttctc atgtttttca
    40081 tctgtttggg cttaatgccg tagatgcaag cttttgaata ccgtttttat cagaggtcat
    40141 gaaaaagctg tttagcaaca agtaacgcaa tgattttgaa gtgataagga gatgtggctg
    40201 aaaaatgccg catctccttt tttattccta aatatgaata cgcattattt tcaatctttt
    40261 tgtagaaaaa gggtacaata gaaaaagaga aaggcgggag aatatggtta tcaatttcat
    40321 tattttattt ttgatgattg caattacttc attttttgta gcaagtgaat ttgctttagt
    40381 gaaaatcaga cgatcaagat tggaacaact agaaaaggaa aatgtaaaga acgccaagct
    40441 agctcttcat gttacacacc atttagataa ctatttatca gctagtcaat taggaattac
    40501 gctaaccggt ttgatcatcg ggtgggtcgg tgaaggatct gtcgcggctt tactagaacc
    40561 agtgatcggt aaactacctt ttagttcggc tattagcagt acaatctctg tggcgctggg
    40621 atttatttta gttacgtata tcgatgtggt tattggcgaa ctgcttccta agagctatag
    40681 tatcgtcaat acggagaagg tcgtcttgtt cgtagttaaa ccacttcatt atttttataa
    40741 agtaatgttt ccttttattt gggtattgaa tcattcagct gcagggctag ggaagctatt
    40801 aggtgtgcgt ctcgtatctg aaggagaaga gacattgtct caagaagagc tgacattagt
    40861 cgcattgaat tcttatgaaa aaggagagtt gaccaaagaa gaatattatt atttggaaaa
    40921 cgtgtttgaa tttgatgata ccttggcaaa agatatacaa gtagaccgta cgtccatgca
    40981 agtattcgaa gcttcagaaa ccgtcaaaga agcgataaaa gcttcgattg aacaaggaca
    41041 tactcgctat ccggttatct tagaatcaaa agacaatgtt ttaggttatg tcaccttgcc
    41101 agatttgatc aagcaatctt ataaagatga ccagctgaca gtagaacagt tgattgaaga
    41161 gccgatcgta acaactgaaa cgatccctat aaaaaaatta ttgacgatca tgcgaaaaaa
    41221 gggtaagcat attgctatct taaaagatga atacggaggg acaagcggct tagtgacgat
    41281 tgaagatatt ttagaagaaa tcgtcgggga aatccgagat gagacagatt tggacgaagc
    41341 attgatagcg gaacagtcag atggctctta tatcatctcc ggcaaactaa cattagatga
    41401 tttccagcgt tattttcatg tagagattcc agagtttgaa gaaacgaatt ttactacact
    41461 tgcgggcttt gcttctagcc gatataaaga aataaaagca ggaacaatca ttgagattgc
    41521 atcattccgt ttcacggtat tagaatacca gcatgcacat atcgattatt tcaaagtaga
    41581 gtccacggaa agaaaaacag aataaaagaa aaaaggctgt gacatttttt gtcacagcct
    41641 ttttgttatc taaaaaacct gttttcggta aacgaagaag atattttcaa taaaagtttt
    41701 catttttagc cgcaacgatt tcttttaatc aaggctatct tgcttctgca tgttctaatg
    41761 tgaaatgttc ctcttcaccc atgtgtttca attgtttcat cgaacgcata taagagtaaa
    41821 gcaacacggc aacagaaccg atccaagcta aaggagaagc tgctgctgct cctgggtatc
    41881 cccacattcc tgtaagaacg atagctgcaa aagaacgcat aagcaattcc atcataccgg
    41941 cgatcgtagg gatcttgctt tgtccaagcc cttgaagtgt gtagcgtaaa atgaacagga
    42001 ctgctaagat ccagtaaaga gacccattga tattgaagta ggtttgtgct aattcaaata
    42061 cacgtgtttc tgatgggtta acaaacaatt gaacaaagaa atgaccgaag aaaatgacga
    42121 tagcacctgc taagagacta aatccgacac tcatcagtaa agtttgacgt accccttgca
    42181 agattcggcc atattctttc gcaccatagt tttgtgcagt gaaagttgcc attgcaatac
    42241 caaatgacat catcggctga tttgcaaatt ggtcgatacg accagcagcg gcttgagcag
    42301 ctacgacatc tgtccctaga ctatttagtg ctgcttgtaa cacgatagca ccaacagcga
    42361 tgatcgaaga ttgaaacgcc attggcaaag cagcatttag gtgtacagca aactcttctt
    42421 tttcaaattt gaaatctttt ttgcgtaact gtaacaaagg catttttttc ttgatataca
    42481 ctagacacag tacgctggaa gaaacttgtg cgattactgt agcaatagct gcaccttcca
    42541 cacccatgcc aaaatagata ataaagatca aatcaagtac gacgttgatg atgaccgcaa
    42601 tgatcagaaa gaatagtggt gtgcggctgt caccaagtgc gcgaatgaca ttagaaagca
    42661 aattgaaact gactgatgca aagatcccta ataaaataat tgaaataaat gtttgtgctt
    42721 gatcaagtat ttcaggagga gtttgcatca attgaagcat cggacgaata aacaggagac
    42781 ttaggacagt taaaatcaca gtgacgacaa gactaatgac aacactagca gcaaaacttt
    42841 tcttcagacc ccgataatct tttgcaccgt aacgttgtgc ggtaataatg gctaaaccag
    42901 ctgttaaacc ttgggcaaag ccaatgatca aaaatgtcag gcttccagtt gcaccaaccg
    42961 ctgccagcgc atttttacct agcgtctgac ccacgatgat catatcgatc atattgtaga
    43021 actgctgaaa aacatttccg atcagtaaag gaacagtgaa catcaaaatc agtttagcgg
    43081 ggcttccttt tgttaaatcg cgcatctttt aaactcctta tatctttaaa atttagttag
    43141 tagtatatag cgtttatcct gagaatacca attagaaatc aatgataatt tatgaaactc
    43201 cataaaaaga agccaagaat tgagtcaaag aatcaacaac ttggcctggt ctgtaaaata
    43261 agggacatcc acgtttatct atcaccttaa tattgtctaa tctcacttgg taaaaggatc
    43321 gtttctctcg taccgtcatc ataggcgtat acttgagcag gaaattggtt cgaataggca
    43381 aacggcaaat caatatccat ttccacattg cttcctgctt gagaaaaatg catcgtcact
    43441 tttccacgat tttcgatcaa atcaaaggtt aatatatttt ctaatggaat gactccctta
    43501 agatctaggt caataataaa ccatatactg tcaatcaatt catctggcaa acttgatacg
    43561 acaccaactg aagcgtaacg gttgcggctg ctatcaaaac tttcaaacat ctgtttaacc
    43621 tccttcgaat caacgaatgt tttattttta taattaatcc taggataaac tagaaatact
    43681 tacttcaacg agggttcatt ctaataaaga atcaggaaaa atgcaacaat aattgaatac
    43741 tttttttcaa agtataaatt tttataaagg tacgataaaa gtatgcgagg aaaagcttgg
    43801 taaattagga tttttcgttt gtaaagccat ttacaaaagt ggaagcccca tatgaaaata
    43861 aacgattaga aataaaaaag gcgtgttaaa agctgctgac ccgccgcttc taacaccacc
    43921 tgattgtttc agtattattc ttctgttgaa ccgatcactt ctggttcgcc accttcacct
    43981 gtgctttctt cttcttcact ttgagcgact acagcagcga tttgctcttc actgtcggtc
    44041 acgaatttgt aatcgtcgtt ttttggaaga tcggctactg taatagaatc acctaattcc
    44101 aagtttgtca cgtcaacttc aattcgttca ggcaatttgt ctggtgtagc agatacgact
    44161 actgtataca agttttgagc taacactcca ccagatttag cacccactga ttcgccaaca
    44221 agaacgactt ctgtttcgac ttctgtttct tccgacatgt ttacagatag aaattctgca
    44281 tgttcgaaat gattggtaaa agtgttcgtt tggatcttgt gaaccaatgt attgactttt
    44341 tgtccatcga tgtccatcgt gatgactgtg ttcgctccat gttcacgcaa caatttgctt
    44401 agtgtcaaac catcgaatga gataggggta ctttctactt tatacccata aacaacagct
    44461 ggaactttcc cttcgtgacg aagtttgttt cttaatgaac ggggacgtac ttctctttta
    44521 cttacttcta atgatactga cataaccaaa attcctcctt aaaatgtgac tgtctgcttt
    44581 ggtaacggac agattttatt taagtgaatt ctaaaggcta atctcgtgca catcatcgaa
    44641 ggcagggcgc ttaaaaaagc gcaaaaggag agatgcgcgc atctcccctt agaaagtcag
    44701 gattcaactt ttctccacca ggtcattacg ctgtgtatga gaaatccaat tcactttact
    44761 atttaaccgt aacgtgaact gttaattaag tcaaggaata actatgatga tgtatttgca
    44821 agcaagaata ttcaaggtat aattaattag tagtaataag aaaggaaaca gcatgcgttt
    44881 agataaatta atagaagaaa aattagaaac gacacgcaag gaaatgaagc ggctgtttgc
    44941 tttgaagcaa gtgatgatag atggcacagt cgaatttcga caaaatcgaa atgtagatag
    45001 tttactgcat caaatcgaag tagcaggtga acacttagaa acaaatgagt cctattatct
    45061 tctcaataaa cctgaaaaag ctgttacagc tgtatcagat ccagaaaaga cgacagtaat
    45121 cgatctgatt gcaagtgccg atcgtttcca accattatat ccagtgggaa gactagatag
    45181 agatacaaca ggactattgc tgatcacttc gaatggacaa ttgggatatg aaatgctact
    45241 tccagaaaaa aaggtttcaa agacttatca agtagttgtg aacgaaaggg tgacaccggc
    45301 agacgtaaca gcatttgaaa atggaatcgt attcgctgat gggtatcaat gtaagccggc
    45361 tgatttaaaa atactcaaag cagacaagca ccaaagtacg gttttattga cgatccaaga
    45421 aggaaaattc catcaagtca aaaagatgtt cttagcccaa ggaaagaaag tgaccgcatt
    45481 gaaacggcta agcatgggac cattggaatt agatgaacag ctaggagtag gcgagtatcg
    45541 atcgttgaca ttagcagaga tgaaaaagtt aagtatttat tttaaatagg aggtcaaaaa
    45601 gatgaaatat aaaactttat tgtttgatgt agatgataca ttgttagatt ttcaattaac
    45661 tgaagccaat gctttaagtg ctttgtttgc agaacaagga atcacattga caccagaaat
    45721 cgaaaccagc tataaggctt tgaatcatca tttatggcag gagtttgaaa aaggcagact
    45781 atcacgagaa gaaataatag gcagtcgttt tgggttactt ttccagcaat ttgggaaaac
    45841 agtagatagt gatcaaatgg aaaaacgcta ccgccactac ttgaaccaag gtcatgatct
    45901 tgttccagga agtctagaaa tcctgaaaaa ggtccatccg catgctgatc tttatattgt
    45961 gacgaacggt gtgtcgcaca cgcaacatcg gcgattaaca gatgcaggaa tgcttgatta
    46021 cttcaaggat atctttgtct cagaagctgt cggagcgcaa aagccgatga aagaattttt
    46081 tgatcatgtc ttttcccaca ttcctaattt tgataaaaag caaactgtga tcattggtga
    46141 ttctttgact tctgatatca aaggaggaaa tatggcaggg atcgatacga tttggttcaa
    46201 tagaaataaa ttaccagaaa tcgaagaaat ccagcctact tatcgaatcg attcattaga
    46261 ggaactttat tcattattgg aaattgaaaa ggattaattt tcagaaaaat atagcatttt
    46321 cgtgtttttt cgctataata gattagaatg aaaaaacgga tacgaaagag gttttagtca
    46381 atgacaaaaa gcaagccaat tatcattggt gtgacgggtg gatccggaag cggaaagaca
    46441 agtgtcagcc gtgcgatttt caacaacttc ccggaccatt ccatcatgat gcttgaacaa
    46501 gattcttatt acaaggatca gagccatttg agttttgaag aacgattgaa cacaaattac
    46561 gatcacccat ttgcctttga taatgatctg ctgattcagc atgtaggcga cttactgaac
    46621 tataaagcca ttgaaaaacc cgtgtatgac tatgtagcgc atacaagaag tcaggcaacg
    46681 atcattcagg aaccaaaaga agtgatcatt ctagaaggaa tcctgatttt agaagacgaa
    46741 cgtctgcgtg acttgatgga tatcaaagta tacgtagata cagatgatga cattcgtatc
    46801 attcgtcgta tcaaacggga tatggaagaa cggggacgca cattggactc cgtgatcgaa
    46861 caatatttga cagttgttaa accgatgtat catcaattta tcgaaccaac gaagcgctat
    46921 gcggatatca ttgttccaga aggcggagaa aaccacgttg ctattgattt gattacaaca
    46981 aaagtagcta gtttcttaaa ccataaatag tgcgcagcga aacagttgaa cggactgcga
    47041 ccgtttgaaa agcaaggatt ctgctttttg aacagtcacc agccaactgt ttttttattt
    47101 ataacaagag gttgtgaaaa cagcatcttg acctaagtat taaaaagaag aaacggaaaa
    47161 tagctgttta tattttaccg ttttttgagc taataccgaa ggtcagctga ttgagcaccg
    47221 tgaagtagag gttgtaaaaa agctgtcttt gcatcaagcg ataagagcat ccaaataaaa
    47281 cagctcctca cgtttttcgt tgggttgggc ttattatcgt agatgcaagc ttttgaacac
    47341 cgtttaacga gaggttgtaa cagaagtggt cagatgcaat aaataagaag aaacttacga
    47401 aaattatttc tcgaatttgt gtgagatttc gacttatttt tttgaagaag ctcttttgtc
    47461 gcacccccta
//
LOCUS       DACDIP010000015        45067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDIP010000015 DACDIP010000000
VERSION     DACDIP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.015"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(14..1024)
                     /locus_tag="IV257_001019"
     CDS             complement(14..1024)
                     /locus_tag="IV257_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8814166.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1204..1548)
                     /locus_tag="IV257_001020"
     CDS             complement(1204..1548)
                     /locus_tag="IV257_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8814167.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1541..1975)
                     /locus_tag="IV257_001021"
     CDS             complement(1541..1975)
                     /locus_tag="IV257_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8814168.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2113..2850
                     /locus_tag="IV257_001022"
     CDS             2113..2850
                     /locus_tag="IV257_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814169.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2843..4054
                     /locus_tag="IV257_001023"
     CDS             2843..4054
                     /locus_tag="IV257_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814170.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4261..5040
                     /locus_tag="IV257_001024"
     CDS             4261..5040
                     /locus_tag="IV257_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8814171.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5110..5772
                     /gene="trmB"
                     /locus_tag="IV257_001025"
     CDS             5110..5772
                     /gene="trmB"
                     /locus_tag="IV257_001025"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8814172.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5870..7624)
                     /gene="ade"
                     /locus_tag="IV257_001026"
     CDS             complement(5870..7624)
                     /gene="ade"
                     /locus_tag="IV257_001026"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8814173.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7756..8685
                     /gene="cpaF"
                     /locus_tag="IV257_001027"
     CDS             7756..8685
                     /gene="cpaF"
                     /locus_tag="IV257_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8814174.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8642..9691
                     /locus_tag="IV257_001028"
     CDS             8642..9691
                     /locus_tag="IV257_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8814175.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9688..9966
                     /locus_tag="IV257_001029"
     CDS             9688..9966
                     /locus_tag="IV257_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8814176.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9941..10411
                     /locus_tag="IV257_001030"
     CDS             9941..10411
                     /locus_tag="IV257_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8814177.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10428..10730
                     /locus_tag="IV257_001031"
     CDS             10428..10730
                     /locus_tag="IV257_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814178.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10747..11118
                     /locus_tag="IV257_001032"
     CDS             10747..11118
                     /locus_tag="IV257_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8814179.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11090..11449
                     /locus_tag="IV257_001033"
     CDS             11090..11449
                     /locus_tag="IV257_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814180.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11621..12628
                     /locus_tag="IV257_001034"
     CDS             11621..12628
                     /locus_tag="IV257_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8814181.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12653..13837
                     /locus_tag="IV257_001035"
     CDS             12653..13837
                     /locus_tag="IV257_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8814182.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14274..15857)
                     /locus_tag="IV257_001036"
     CDS             complement(14274..15857)
                     /locus_tag="IV257_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8814183.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16435..16578)
                     /locus_tag="IV257_001037"
     CDS             complement(16435..16578)
                     /locus_tag="IV257_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8814184.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16801..17280)
                     /locus_tag="IV257_001038"
     CDS             complement(16801..17280)
                     /locus_tag="IV257_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8814185.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17353..17637)
                     /locus_tag="IV257_001039"
     CDS             complement(17353..17637)
                     /locus_tag="IV257_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814186.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17715..17909)
                     /gene="ssrS"
                     /locus_tag="IV257_001040"
     ncRNA           complement(17715..17909)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV257_001040"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17981..19267)
                     /locus_tag="IV257_001041"
     CDS             complement(17981..19267)
                     /locus_tag="IV257_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8814187.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19405..20076
                     /locus_tag="IV257_001042"
     CDS             19405..20076
                     /locus_tag="IV257_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8814188.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20042..20530
                     /locus_tag="IV257_001043"
     CDS             20042..20530
                     /locus_tag="IV257_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8814189.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20543..21490
                     /gene="prmA"
                     /locus_tag="IV257_001044"
     CDS             20543..21490
                     /gene="prmA"
                     /locus_tag="IV257_001044"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8814190.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21491..22255
                     /locus_tag="IV257_001045"
     CDS             21491..22255
                     /locus_tag="IV257_001045"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8814191.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22488..24701
                     /locus_tag="IV257_001046"
     CDS             22488..24701
                     /locus_tag="IV257_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8814192.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24712..25158
                     /locus_tag="IV257_001047"
     CDS             24712..25158
                     /locus_tag="IV257_001047"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8814193.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25329..26588
                     /locus_tag="IV257_001048"
     CDS             25329..26588
                     /locus_tag="IV257_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8814194.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26677..27624)
                     /locus_tag="IV257_001049"
     CDS             complement(26677..27624)
                     /locus_tag="IV257_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8814195.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28018..29310
                     /locus_tag="IV257_001050"
     CDS             28018..29310
                     /locus_tag="IV257_001050"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8814196.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29325..31094
                     /gene="aspS"
                     /locus_tag="IV257_001051"
     CDS             29325..31094
                     /gene="aspS"
                     /locus_tag="IV257_001051"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8814197.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31203..32078
                     /locus_tag="IV257_001052"
     CDS             31203..32078
                     /locus_tag="IV257_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8814198.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32344..34227
                     /locus_tag="IV257_001053"
     CDS             32344..34227
                     /locus_tag="IV257_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8814199.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34302..34586)
                     /locus_tag="IV257_001054"
     CDS             complement(34302..34586)
                     /locus_tag="IV257_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8814200.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34576..35310)
                     /locus_tag="IV257_001055"
     CDS             complement(34576..35310)
                     /locus_tag="IV257_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8814201.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35439..36062
                     /locus_tag="IV257_001056"
     CDS             35439..36062
                     /locus_tag="IV257_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8814202.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36254..37375
                     /locus_tag="IV257_001057"
     CDS             36254..37375
                     /locus_tag="IV257_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8814203.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37382..40510
                     /locus_tag="IV257_001058"
     CDS             37382..40510
                     /locus_tag="IV257_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8814204.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40529..43723
                     /locus_tag="IV257_001059"
     CDS             40529..43723
                     /locus_tag="IV257_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8814205.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43869..44576)
                     /locus_tag="IV257_001060"
     CDS             complement(43869..44576)
                     /locus_tag="IV257_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8814206.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14482 a         7985 c         9429 g        13171 t
ORIGIN      
        1 tttttgtttt cttttatttt gatgaatcag ttgtttcttc agttgaagat tcttttgttg
       61 aatcagttga ttttgtgtca gaactctttg ttgaagctgt agtttcactt gagtctttcg
      121 tacttgatga agttgttgtg aatgcactca acacattttc aaaggcatca tctttgattt
      181 ttacatttgc atcttttaat tcttcaccga ttactttagt tgtgaatgca ttgtcactta
      241 gtttagtttc agtagcgata tcttttagtt gatctttgta tttatccatg tcattgcctt
      301 tattttggtt tttcaccatt ttgacaacgt agtattctgt cgcataagaa gtaggatttg
      361 ttgtagtaat aacatcggag atctcaccgt ctttcaattt gaaagctgct tcttttactt
      421 ctgcaggaat agttgttgtc gttgagtcga atttaacttt accgccatct tcttttgtct
      481 ctgtatcagt tgatttgtct ttggctaatt tagagaaatc atctccatca tccgctgatt
      541 ttttcacatc ttttgcttca tcttcacttg ataatttgat gatttgtgct tctacttctg
      601 gatggaaaga tttccaagca gttttcaaat cgtcatctgt aatatctacg tgagctttca
      661 agccagcttc aaaagctaaa ttgttacgaa tatattcttt ataagtatct ttcgtatatc
      721 cagcagcttc taattgtgat tcaaaagtat cacctaatga tttcgcttgt ttgtcgtatt
      781 ctgcatccac ttgcttatcg gttactttat cgccatattt gttattgaat actttataaa
      841 tgatcatccg ttgtactaag gattggttcg atgattcaag ttttgcttca tcatagaaat
      901 cagatacagt gatcgttccg cctttcatcg ttgcgatatc tttattagta tcgcctgaac
      961 aagctgctag agttaaaaca gccaacgcac tagtagctgc caaaataatt gatttttttt
     1021 tcattaaaat ttgcactcct gtaattagtt attattttta tatattaact ataccatatc
     1081 ccatttcctt taaaaacgtt gtcgtggtag tttcaaacaa aattcaaaat ttcatcacat
     1141 tcctaggaaa tacgcaaact tttctcactt atatagttaa atctgttgag aaaataatca
     1201 ttattatatt tgttcaggat actctggcag atcagctttt agtttttcta cttgttcatt
     1261 gatttgtgtg atacgtggtt cggcttggaa tttaaaatct tcgatatctt ttttgattgc
     1321 ttcttggaaa gcaggaatca cagattcagc tgtttctttt gtttcgatca atgcgctttt
     1381 aaaatgattc aggctgttat ttaaatcatt tgtcagagtt gttgcttctt ccaattcttc
     1441 gatcagtttc tttcttgttt cattcccgct cctgggcgcc aataacagac ctccgacagt
     1501 gccagcagca gctccaaata acagtccttt gaaaaagttt ttaatcatcg caggacacct
     1561 gctttacgat tgtttcttga atagctttta gttcttccga tccataacta tcttgatgat
     1621 tgacaaagtg aatagaaaaa tcatcctctt tgctgtagcg agggactaaa tggatatgtg
     1681 aatgaaaaac agattgatag gctagttcac ggttgttatt caagatatta agacctttca
     1741 tttctggaaa agctttttcc aatgcttgag caatcttagg cacacgcgaa aaaacctctc
     1801 ctgcaagttc gggatcatat tcaaaaatat ctgctacatg acgtttaggc acaactaatg
     1861 tgtgtccttt cgttgcttga gagatgtcta aaaaagcata gaccttgtcg tcttcatata
     1921 ttttatagct tgggatttct tgattgatga ttttgcaaaa aatacagttt tccatataaa
     1981 acatcctttc tataaagaat aaatacttca gcttaacttt accataagtt aaagctattg
     2041 taaggaaaaa aacaaaatac cgtcattttc ttcgtggtat aataaataaa aataaatggc
     2101 ggaggaaaaa gaatgagttt aattatcgat catgtcacag gggggtacgg acatctccct
     2161 gtgttaaaag atatcagttt cgatgtacag cctggagaga tagtagggtt gattggactg
     2221 aacggagcag ggaaaagtac gaccatcaag cacattatcg gtctgctgga agcttcaaaa
     2281 ggaacgatag aaatcaatca tctatcctta aagaaaaata cggaagaata tcgcaaacaa
     2341 attgggtata ttccagagac tccttcttta tatgaagaat tgacattgag agaacatatc
     2401 gaagtcactg cgatggccta cgatatacct atggatatag caatgaaaag agcagaaaag
     2461 ttgttggaga cttttcggtt atctaatcgt cttgactggt tcccagctaa tttttcaaag
     2521 ggaatgaaac agaaagtgat gatcttatgc gcctttttga tcgagccttc tttatatatc
     2581 atcgacgaac catttttagg cttggatcca ctagctatca atgcattgct tgaattgatg
     2641 gtagaaatga aaaaagaagg agccggtatt ttgatgtcta cgcatatttt agctactgca
     2701 gaaaaatatt gtgatcgttt tgtggtctta cataacggag agatacgagc agacggttct
     2761 cttgagatgt tgagaaaaga atttcagctg ccggattctt ctttggacga aatctatatt
     2821 gctttaacaa aggagcaggc acatgagtag tttttttcaa aaacgtttag caagacatca
     2881 aaagaaaatg atgaaatata tgcgttatgt attgaacgat cattttgttt tagtttgtct
     2941 tttcttgatt ggcggtgtgg gattatatta ttctaattgg ttgaaaaccc tgcagccacc
     3001 gtttgcaatt ggagggctca taatctctgt attttggatg ctctgtttat tcgtgggtaa
     3061 gatggctact tttgcagagg cagcagatat tgttttttta cttcctaaag aaaaagaaat
     3121 gcaaacctat ttggaacgag cttttcgcta ttcttgcatt tttccgttga tctttttgat
     3181 attggcatgc ggattggcca tgccattagt ggtggttagt acggagcaat ccttttcgat
     3241 gtttttcgtc tatctgatca tattatggag tttgaaattc agtcatctgc aaatcaagag
     3301 agcagaattg tttcgactag atcaacaagt tattcgttat tggaaattag gctggttttt
     3361 ttcttctctg atctttttgt ttctatcatt ttattggcta atatgggcag gaatagcagg
     3421 tgcattagta caggcaagtc tatattactg gtttttgtgg aagcaacaag aaaaaggatt
     3481 ggattgggag aaaatggtgc atgtagaaga acataggttg catcagatct atcaatttat
     3541 caatttattc acagatgttc ctgaaattac ggcaaagatc aaaagaagac gctatttgga
     3601 tcctatattg actcatatta aaagagaatc aaaaaacact tatctttact tgtttgcaag
     3661 aagattggca agaggttctg attttagtgg tctttatttc cgattagtaa ttataggtgg
     3721 gttattgatt gccggagtag tcgactttta ttttagtctg attatcggtt tcttattttt
     3781 atatttgatc ggtttccaac tgttaccttt agcgaaccag ttccgctata tggttctcgt
     3841 tcagctttat ccaatccata agaatcagaa gaagcaggcg attcagtatc tactcacatg
     3901 ggtattaatt atcgcgtcgg ttatttttgg tttgattgct gcagtcgtat tagatgggca
     3961 gcagcgatgg gttccgctag cggcttattt gattgttact atgctctttg ttaaactata
     4021 tgtaccaact cgtttgaaga agatgtcaga ctgaaaacaa agctgtgtag ggtaaaaatg
     4081 agaatgaaaa atagagcgtt gacagtaaga atgctgttcg ttctattttt tttgagaaaa
     4141 gctggttttt tgaatggcaa ttaaatgaaa aagaacgtat atgtgcttga cgtgtagtgt
     4201 tcaggcgact agaataataa gtactgcaaa acaaagagta agaggacggg agaatgcatg
     4261 atggagtttc agttagacca agagtggcgg ttacgtccca tcaaaggcga cacaggaaaa
     4321 acatacatag gcatgcgtga tgaggacaaa gtctttatta aacgaaacac gactccgatg
     4381 ttggctgctc tttcaaggga aggaattaca ccaaaacttg tttggacaaa acgaacgggg
     4441 aatggcgata ctttgacagc acaagagtgg ctggaaggtc gcttgctgac tcctaatgag
     4501 attggtcaaa gaaacgacgt gattgatgtt ttgtaccatt tacatcattc aaattctctt
     4561 aaaaccatgc tgacccgtat cggtggtcgt gtcatgacgc cggagaagat gctgcaaaaa
     4621 tatgaaaaag aattaccgga agaactccag aaaaatcaat ttctgcaaat tgtccatcgt
     4681 tttcttcgtg agcagatccc gtcaatagat aaggaaaact tatcagtagt acacggagat
     4741 gtaaaccatc gtaattggtt agtatgtaaa aattatttat atctagtcga ttgggattca
     4801 atcatgtttg cagaccctgc ggtggatatt gcaacgattt taggaaatta tgtgccactg
     4861 tctagctgga atcagtggct gatcagttat ggcatacgcc cgactgacga aatgcttgaa
     4921 aaatttcatt ggtatgcggc catgaatttg ctccaagaaa tcaccagata ttgcaaacgt
     4981 gaagatcatc gacggatgaa cgaagaaatt ttacaattaa aacgaatttt cagtggctga
     5041 ggtttggccc aaactctgcg gagatttggc cagacctttt taattagagg aaaggaatgg
     5101 agataaataa tgcgtgtccg aaatcgtccg ggagcagcgg aaatgttagc ggctcacccg
     5161 aattttgtaa ttagtgatcc aacattgtgg aagggaaaat ggaatgaatt gtttgaaaac
     5221 gatcatccaa tccacattga gattggtatg gggaaaggcc agttcatcac tggaatggca
     5281 aaagcccatc ctgaaatcaa ttatattgga gtagaaatgc aggtcagtgt tgtttcgatt
     5341 gcactagaca agttgatcga acaaccgctt cctaacctga aacttctaca tgtggatgga
     5401 tcagcgttga cagagtattt cgctgacagc gaagtagatc aaatctatct gaatttttcc
     5461 gatccatggc caaaaaaacg tcatgaaaaa cggcgattga catataagac ttttcttgct
     5521 gtagatgaac agattcttcg tccaaatgga gaaatccatt tcaaaacaga taatcaagga
     5581 ttgtttgagt attctctggc aagtttttct caatatggca tgatcctcaa acaggtatgg
     5641 ttagatttac atcaaagtca atttgaaggc aatattatga cggagtatga agaaaaattt
     5701 tcttcaaaag gtcaacgtat ttatcgagta gaagctcaat ttcaagataa gaccaaaaag
     5761 aaaagtctgt gaaactaact ttctgctttc ataaaaacat taatatccat aaataaatcc
     5821 cagaaaatga acattacaga tgtaatgtat tttctgggat ttatttgatt taaaatgtat
     5881 ttatttcatc ggtttttcca caaggtggaa tcaattggaa gcgagaaaat gaaaataatc
     5941 ctttgtcggt gatcttcaat tcgggtatga cagataatgt taagaaagaa agtgtcaaaa
     6001 aaggatcaaa ggttttagct gctcctagtt gatgtgcttt agttattaat tcattcaact
     6061 gttcatagac gatcgaaaaa ggttcctgtg tcatgattcc gcctatagga agagaaacac
     6121 aagctatttc ctgctgtttg ttgacaacgg tcatcccgcc acctttttga atcaaaagat
     6181 tagcagcata cagcatatcc tcgtcgtttg tcccaacgac aactagatta tgcgagtcat
     6241 gggcaatcgt tgtcgctatc gctccttgtt tcagctgaaa tcctttcact atgcctaatc
     6301 caatgtttcc cgtttgatgg tgacgctcga tcaccgcaat ctttaattgg tctttttcga
     6361 ttgaaggaat gaatcgttcg ccttgtcggt ccactttttc taccaaatct ttagtcacta
     6421 gactattcgg gataatttcg atcacatggg cttgtgaaga agaaagaagc agctctaacg
     6481 aatgtggatt taatggttga gcgtttagtt ttggcagaca acacgtatat gaatttgaat
     6541 gatcagcaaa taaatggtct tggagttgcc cagattgaac aactaatgtt cccttataga
     6601 aaacttcatg aattggcagc ttggtaagat cgtctgttaa gaagaaatca gcttgatacc
     6661 caggggcaat cgcacctagt gttttcaaat caaaacattc tgctgcattc aatgttgcca
     6721 tctgaatggc ttgtaatgga tcgattccaa gtgcaatagc ttgacggacg atatgatcga
     6781 ttgatccttc ttttactaaa tcattgatca gtttatcatc ggtgacaaag aagcatcttc
     6841 ttgaattttc aggtgtgatc gccggtagta gcgatgccag atcttttgca acggtccctt
     6901 ctcgaaccat taaatacata ccaagatcca aacgttcttt agcttctttt gcagttgtag
     6961 cttcatgatc cgtccgaata cctgctgcag gataaacatt taactcttcc atgccgatac
     7021 cagctgcatg cccgtcaatt tttttcttat gctggtgcat caattggatc ttttctagga
     7081 tgtctgtttc attatttgct actgcttgat agttcatgac ttcagctaag cctaaaactt
     7141 cgggtttttg taaaaaaggg gctaattttt ttgcatttaa atttgcacca ttggtctcaa
     7201 aaggggttac aggtacacaa gaaggtagca taaaaaagat attcatgagt gtttgtcttg
     7261 catcttctat cataaactca agacctgtac tcccagcaat gtttcctatt tcatggggat
     7321 ctgtcactac agtagtaact ccatgaagca aggatacttt tgaaaattcg cttggtgata
     7381 aaagtgagct ttcgatatgc acatggccat cgatgaatcc tggcactaga tattgcccat
     7441 tagcattatg tacgcttttt ccttcgtatt caccgattcc tacgatcatt ccttcgcaga
     7501 ttgctacatc tttttccatg atttctttcg taaagacgtt gacgatctta gcatgcttga
     7561 tcactaggtc agcaggcttt tttttacttg ctacttcgat atatttcctt aatgttgttt
     7621 ccaatggagt gcctcccaag ataaattttt ataagtataa cataaattcg gtttttgcca
     7681 atattttttt gtaaattcat tttatttgaa acaaaaagat caaaaagacg tatgtttttt
     7741 gtggagggga gaataatgga aataaaacga ttatcggatc aaatgataag tgaaagtttt
     7801 gccaaacatg cccaagacgt atatatattg ccagcttcag aagtatttta catctatttt
     7861 cgctatggaa aaaaacgaac attctggaag aaagtctctt cggcagatgg acagcaactg
     7921 atctcaagat ttaagtattt gggacagatg gatatcggag aaaaaagaaa agctcagtta
     7981 ggtgcattta cttattcttt agaaaaacag gaagttcgtc tgcggttgtc ttctgtagga
     8041 gattaccttc aacgagagag tttagtgatt cggctattat acggtacttt tgaaacaccc
     8101 catcgttgct ttagttctcg agattttgca actattttac agaaaaccgt cagacgtgga
     8161 ttataccttt tcagcggacc agtcggatca ggcaagactt ctctaatgta tcggttggct
     8221 ttggaagaaa aattgcaagt gattactatc gaagatcctg tagagataga agaaccgttt
     8281 tttctgcagc tacagatcaa tccgaaaata gcacagactt atgatacact tttgagactc
     8341 gcattacggc atcgtccaga cttgctaatc attggcgaga taagagatga atcaactgca
     8401 catgctgcgg taagagcagc actaacaggg caccgggttt ttgcaaccgt acatgcacgc
     8461 aatcttgaag gtacattggt cagaatccat gaactgacaa agaaaaaaga agagctatct
     8521 gaatgtttag ctggtgtcat ttatcaagaa cttttggaag atgccaatca ccatccttct
     8581 gttttatgga gttatcaatt tatggaggaa gaatcagtaa aaaaatcatg ggaggaaagt
     8641 tatgaagaag ctcaggaaaa tatttggcag tcagaactat tctaaaggat taacaagaag
     8701 ccagcaaaac cagtttgcac aaaccatggg agaactgctt ttatctggtt ttagtctgca
     8761 agaatccctt tatttattac tcacgatccg ttcgtttcca cctgttattt tggcaaatat
     8821 ccaacatcag ctaaaggaag ggcaagcgtt tagtgaggta ctttcgcaaa taggatatgg
     8881 agcagagcaa ctggtacaga tcgagttagc tgaacgtcat gggaatctgc cgacaacttt
     8941 gttggaaatt gccaaacagt ttcggcttgt tgaaggattt cgcaatgatt tgaaaaagct
     9001 gcttgcatat ccgatgctat tgctcacctt tttgctaggt atcttgttta gtatgcggtt
     9061 gttcgttttg cctcagctta ttggttcagg aatggtcatg caagagcatt ggggtattca
     9121 gctgatacaa gtctttcatt ggtatgttct actattcatc ctagcggtta ctctattggc
     9181 tttactagta aggtatcgtc tgatgaagtt gtctcctgtt gatcagagtg aatggataag
     9241 cagacaatta ttgattggac cgatatattc cttgtatcaa tcggcttatt tgtcactaga
     9301 gattggaaag ctttttcatg aaggtttcga attaaggcaa atcatagtct gcttaaagga
     9361 aacgaaacaa gaaagtctga ttcagcatct tgcctctaag ttgattcaag gattggaggc
     9421 aggtatgtca ttggctgagc agttccagaa ttatcgtttt ctatcagaag aattcagtaa
     9481 aattgtttta cagggagagg caaagggaag tttaggtaaa gaactgattt tttacagcga
     9541 atggacaaga aaacagcttt tcaaacgtct tcatcactgg atgcatttta ttcagccaat
     9601 catttttttg ggcgtagcgg ctttgatttt attagtttat gccgctgttt tattacctgt
     9661 ttacggaagc atagaggagg ttttgccatg aaaaagagaa aaatatctta ttatagcggt
     9721 tttacattga tcgaaatgct aatcgtctta ctgatcatca gtgtattagt gttgttgttc
     9781 gtccctaatt tgtcgcgtta taggaatcat gtagaccaag aaagtcgtga agcgatcatc
     9841 cagttggtag acacacaaaa agagctctat gcgcttcaaa ataatggaag agttcctact
     9901 gtggaggaat tgttgaatga aggctatatc aaacgagaac atgcagaaat ctatcagcgg
     9961 ccttgaaggt aaggttttag catcaggata tacgttgttt gaatcactct tactgatttt
    10021 attacttaca acgatccttt cttttccagt tctcgcattt tctgcatgga agaatgaatt
    10081 ggcagtctat caatttttcg gccaatttga aaaacgaatc tattctacgc aaaaaatcgc
    10141 tattgtgaat cagatccaaa caggctttta ttggaatagt gaagagaatc aaatcatttt
    10201 tcatgttccg aatccagata aaataaattg ggaagtatta gatattcctg atgagattac
    10261 cttgaaacgc catgcttcta ttacttttgc tgctagaacc gggaatgaaa gttcactaaa
    10321 agcttatcaa ttttattggg aagaaaaaga gcggacgatt acgtatcaat tccagatagg
    10381 gagtggtcgc tatacaaaaa gaattgaata aaacaggatt tgttttgttg gagtccctta
    10441 cagcgcttat gatcgcttct gtctgtgtct acttagtgag ctttggccag ctgtttttgc
    10501 ttcaggcaga aaagaaaagt ggggaggaac tgctatttat tcgaatgatt tatgaagata
    10561 tcaagacgca gcggatgtat cacaaaagac cagagcgaag ggaagaaatg atcccatacg
    10621 atatccaaat tgtcgataat aatggagtaa tcgaaaaagt gtttgtcaat atggagaaag
    10681 aagcgatcag tattgaaaaa acaagtaatt ccagcattta cgctgattga atgtctaatt
    10741 ggactgatga tattgagcag tctattgctc acattcaatc ttttgattca gcaaaccatc
    10801 caagtaagtg aatttttgca gcgtaaagat caaaaagagt ggctaatatt tttagcacaa
    10861 ttagaagaag aactcaagag cagtcacaat gtctctgtga aaaatcagca actgcactac
    10921 acgatcgagg ataaacagta tatcatcgaa agatatcagg caatgatacg taaaagaaga
    10981 acttccggcg gacatcaacc tatgttaaca agtatcactg agcttcaatt attggaaaaa
    11041 gaacacacaa tttcttttta tgtacacttt gaaaatggag aagatggata tgccatatgg
    11101 acaaaaaacg atcagtagaa aatgttataa gggctctatt ttattttctg tcttgctttt
    11161 atttctttta tgtacgtttt tattgatggc agcattagaa agctaccgtt tgtcaatgga
    11221 tcttaatata cgaatcaaaa attattatat agcaaaaata atgaagcaga tggctttatc
    11281 ggaacagtct acgcttgccg aaagttcgga aggagttttt tactacacaa ctggatctgt
    11341 aacatatcag tgggtacaac agtccctatt cttagaggtg gaagtctcac cgtttatctt
    11401 tcggttcaca gaagaagtaa aaaatgatac agatactagt acagaatgat ggaatgttcg
    11461 tgaaaacgcc ctcttttctg ttataacact gtttttaata aaaaaatgtt tgccgtttca
    11521 caaataaaaa acagtgaaaa cgattgaaat tttgtcaatt gttcgataga attaaacagg
    11581 tgaaaaaatt gttataatat atctagaaat gaggtgcacc atgttaccag aaaaaatgga
    11641 acaagggttt aatcaaaacc tggaagctat tcagctttta cagaatgcat tgggaacttc
    11701 ctttttagaa gcctatgtcg aaaatgcaga aaatttaata gatgattatc aagtacgtgt
    11761 ggtggatgga gtgccgacaa aagagacaac gcaacggatc acagctcttt atgaagagtt
    11821 gaagaaactt tctttcgagc cagaagaatg gcgcagactg tctcaattac ttttattgaa
    11881 gggcagccag acagaacatt tgcaggcgaa ccatcaactc actcctgata gtatcggttt
    11941 tttatttgtt tttcttattg aacaactata cacaaataaa aaagcaccgg ttaagattct
    12001 ggatattgcg gcaggaatgg gcaacttgtt attaacagtt cttttaaacc tatcgaatgc
    12061 ggggtaccaa acagaaggaa ttggtgtgga tattgatgat acattgttgg ctgtcgcagc
    12121 ttctacaagt gatttgacac aagcaaatgt tcaatacttt catcaagacg ggttgcagga
    12181 attattaata gatcctgtcg attttgcaat cagcgatttg ccaatcggtt attatccgaa
    12241 tgacgaaaaa gcaaaagagt ttttaacaag taccgaagaa ggccatagtt atgcacatca
    12301 tttattgcta gagcaatcaa tgaaatatgt gaagccggat ggttttggtt tgtttttgat
    12361 gccgagtgga tttttagaaa cggatcaaag tgaagaaatc aaaaaatggt ttaaagaaga
    12421 aggttatctt caaggaatga ttcaattgcc agatgaattg ttccgtaata aacaatcgca
    12481 aaaaagtatt ttgatcttac agaaaaaagg accacaagca caacaagtaa aagaagtctt
    12541 gcttgtgaaa cttgcttcat taaaagaacc agagaaggtg acggaatttt tcaacgagtt
    12601 taaaaactgg aaatcatctg ctctgtaatc attcaacgag aaggagagtc atatgtctaa
    12661 aacaattgca atcaatgctg ggagttcaag tttgaaatgg caattatatc aaatgccaga
    12721 agaaaccgta gttgcgaaag ggatcgtcga acgtatcggt ttacaagatt ctatttttac
    12781 catcaagtac ggcgataatc aaaaattcga acaaatcatg gatatcgaaa accacgaagt
    12841 agcagtaaaa atgctgttag atcagttgat tgaactgcaa attttaagct cttatgatga
    12901 aatcaccgga gtagggcacc gtgttgttca cggaggagaa acttatgggg attcagtagt
    12961 gatcgatgag aaagtaatgg accgcattgc tgaattagca gaatttgctc ctttgcataa
    13021 tcctgcaaac ttaatgggga tcaaagcgtt taaaaagatt ttacctgaca ttttgagtgt
    13081 ggctgtattc gatacatctt tccactcaac aatgccaaag caaaattatt tatatagctt
    13141 accggtgtct tattatgaag atttcaaagt aagaaaatat ggcttccatg gcacaagtca
    13201 tcgttatgtg tcggaacgtg cggcaaaatt attgaacaaa ccgatcgaag aactaaaaat
    13261 catcacttgc cacttaggaa atggtgtatc gatcacagca gtcgatggag gtaaatccat
    13321 ggatacttct atgggattca caccattagc tggggtgaca atgggtacac gttcaggtga
    13381 tatcgatcca gctgttctcc catatttaat ggaaaaatta gagattgata ttgatgaaat
    13441 gatcaatgtt ttgaacaaga aatctggtct gctaggccta acaggattgt ctagtgatat
    13501 gcgtgatctt gaaaagaact atgaaaaaga acatattcgt ttagcttatg acattttcgt
    13561 ggaccgcatc cgaaaataca tcggcggtta tgtgacaacg atgaatggtg ttgatgcaat
    13621 tgtctttact gctggtatcg gcgagaatga cggacatgta cgtagtgaaa tcatcaaagg
    13681 tatgtcttgg tttggttgtg aaatcgatcc agcgttgaat gaattgcgtg gtgaagaagt
    13741 agatatctct acaaaagaat caaaagtaag agtattagtg atcccaacag atgaggaact
    13801 aatgattgcc cgtgatgtgg agcgtctgcg cggctaagcc aaagcattcg ttaggataaa
    13861 agatgaaacg catgcaagag ggaattcttg catgtgtttt ttttgcggag aagaccgcaa
    13921 aatggtaatt taacttcaat ttcccttctt ttttacagga tcttcttagg gaattttgct
    13981 tggaaaagtg gtcaaaaggg aaaagactgg cattttattt attgattttc aggaaaatcc
    14041 caatatttta ttttcattca tctttataag ggacattaca tatgtaaaaa gaaaaaacat
    14101 ttttagatca gcctcgtggg ctgtcgactt tattcttcgc tttatgttta gctagaaaac
    14161 cgatccgaaa cattcattga tcgaataata aaaacttgat tttccacctg tactttgcct
    14221 gaatcgtgaa agcacaggta tgaaaatcaa gttttttgtt gcgaaaaaac gtcctagtca
    14281 atcagttggc cgagcggctg gcggctgatc tgctcctgat caataacgag tgcttcttgg
    14341 aagtgttgct gcaaaaagta gcggtcatgg ctgactgcta ggagcgttcc ttgatagtct
    14401 tgcagcattt cttcaatttc ttctcgagca tagctgtcga ggtgattggt tggttcatcc
    14461 aaaatcagga cgttgatttt ttctaaaaag agtttcagca acgccaaacg gattttttca
    14521 ccaccggata aatctttgat ccgtttaccc acatcgcttt gataaaagcc acttttggct
    14581 aattgccgtc gagcgacttc ttcgtttcca gtcaatgtga tggcgtaagc cagcaaacgg
    14641 ctatcgggat cggcaaactg cagctgctgc ggcaaataac cgatttgcaa gctactacct
    14701 aatttgatgg tgccaccgta aggcttttcc tcaccgagta ataatttgag taaggttgat
    14761 tttcctgcac cattgtcgcc gatgatggct aaatgatctc cacgatagac tgcaaaactg
    14821 ctgtctttga atagtacttg atcagcgtac tgctggtgca agttttgaat gatgaaaact
    14881 tctttgccag attttccacc attagacaag ctttgcagcc gatttttcgg gggaggtggc
    14941 ggtttgacga gtgtcagttt cgccaggcgg cgttccagtt ctttggcttt tttgaagaag
    15001 gactcattgt ctccttcatg tgcccactga cgaaaacgac gaatcatgac tttcaaccgc
    15061 tggacttctt tttgctgcag ttcgtaattt tttgtcagct cagccaagcg agcttctttc
    15121 aagactcgaa aacggctgta gtttccggga tactcaatca atgcaccttc ttccagttcc
    15181 catgtttttg tggtgacttg atcgagaaat tgccgatcat gagaaacgac cagaaccgat
    15241 ttttttgtat tctgtaaata gttttccaac cagcgaatac tgttaagatc taaatgattg
    15301 gtcggttcat cgagtaaaag taaatcggca tcggctgcta aaattttggc taaagcgact
    15361 ttaactcgtt cgcctccgct aagttctgtt aaccgtgctt cctgacggtg tggaatccct
    15421 aagccttgca agatcgctgc aatccggtct tctaatacgt aaccgcctag ttcttcaaag
    15481 cgatgctgca gctcaccgta acgggccaga cacttctcta agtttgtatc tggttctgtc
    15541 atcaatgctt cgtagcgact catttgctgt ttgaggtcct gtatctcagc ggcagaatgg
    15601 agcaagtagt ggttgacggt cgcttcggaa actgtaagtt cttgttcgac cgtcccgatc
    15661 tttaagcctt ttttgcgact gataacaccg ctgtcgacac cttctctgcc ggtcaagatt
    15721 tgtaaaaagg ttgatttgcc agttccgttg ataccgacta aggcaatttt ttcaccagct
    15781 tcaatttgac aggataaatc ctcaaataat ggattcccgc cgattgtttt tttaatagaa
    15841 attgcttgta tgatcatgat ctctctcctt gagaccactc aaaaaggcca tggttatcca
    15901 tggcctgtgt tatttaaact cagattatca tgagaaaaat accttgagat ggccattgat
    15961 tttacgtgaa ttcaactgtt ttggacaaac ttgtcccgtc aacagcaact gtaaaaccaa
    16021 ttgcatggct aaatgcgata tggtatcgtt ggtaattttc attacttgtt attttagtca
    16081 tgcgcatcat agtattttcc ctccttcgtg tagttgtctt ttccagtgta cctgaaagtg
    16141 tcagaaagtt caactagcgc tgacatttac ttaatattta tcgatgtgta attctgtcag
    16201 agagcttttt ttatctgact gagggtaatg tctcgtggac cacatcattg tattctgagc
    16261 taaaccagcg ttcaggcgaa atcatatggt gtttgataaa aaactttgtc tacatgactt
    16321 ccttttgcca tttgcttaat accgttgcat taaacagctc ttctctttgg aaatctcacc
    16381 aacatcgcgt tcaggagtat cgttgtggtt gcaatggcaa gcggtagcct ttcatcaatt
    16441 cttgaatgat ttaccgaatg tagcacttgc cttctcgcgg tacccgaacc cgaaataaaa
    16501 ttttagtttg gcgataaaat cggtatccgc catcgatact tcatattttt tcgtatcaga
    16561 ttcatcggaa acttccacat tgacgaacca ataagctttg gcatgcttag gtcgttttaa
    16621 accacaccag ggcgactaat cgccctggtg ttcacttttt tttattttac ttgaactcat
    16681 tgactgtttt ccctttattt taaacattat aaagaccgat gagtgcttgc cgcgtctttt
    16741 gtttttatca cggttttttg tgcaaaaaaa cctgccgata gttggcaggt cgtctacgtg
    16801 ttaagctttg gtgtggtctt tagctggcaa ttggttttct aaatcagttc ggacaacaag
    16861 cacatcgcaa gctgcgtttc taataacata ttcagaaaca gaaccaataa ataaacgttc
    16921 tacagcattc aatcctgtgg ctcctaacat aatcaaatct acttgattat cttctgggat
    16981 ttctctggca ataatttgtt ttggtgagcc atattctacg acagaggtta cattattcaa
    17041 gccagctttc ttcgcattgc tttcataatc tgccaatgtt tgctttgcca tttcagtagc
    17101 ttgttctgca agcattccat caaaggatga aacggtttgg aaagaacgtg tgtcaatgac
    17161 atgtgctaat agaagttctc cgttatttcg gatagctaca ttgaccgcct ttttaaaagc
    17221 taattcagct tcatcagaac catctacggc aaccatgatt tttttgtatt gttgtaacat
    17281 aagcattcac tcctcaagtc aatcgtattg tactctttta ttgtagaaca ctttagggaa
    17341 aaagagaatt atttactgct ttggttcagc aatttttttt gcaaatcgga tactgaaggt
    17401 ggcagaaaca ataattacga tcagtaaaaa gaccaaggca ataaagcgtt gattgaaaaa
    17461 agctgttaaa atacccatta cgcctaaaac agcatatgca gctccgtaaa attgtaggaa
    17521 tccttgattt ttatcatttt tgttgatcaa tacaaaaaag atatcctgtt tattaaataa
    17581 ataatagcta acgaataata atactaaagt ggcgataatc attaaaatcc taatcatgaa
    17641 caaatcctcc aaaataagca acaaatagaa aaagcaattt gccaagagca aattgcttta
    17701 ttctttaaaa gatggtaatc tccgttaatg ccgttgtttg tcctgtagta ttgatcccgc
    17761 gaattcaagc aggtgggtcc catggtcgta gcttcgaact accaaatgat aaggcatgtt
    17821 accagcagtg acacaagacg ggtcccaaga tatcaataaa aatgttcggt caacacacaa
    17881 agaggacaat tcgaacatca cagatttccc acctctatag tagcatatga atttaaaaag
    17941 agcaatgaga gagctcgcag aaacagaacc gttttcttgt ctagtttttt ggaggatgct
    18001 ttttccactc ttttatacgg taatactgtt gacccagagc ctgttcatat ttccctgtat
    18061 cttttggctg ataatactga gcattcttca gtttatctgg taaatattgc tgattgaccc
    18121 aagcctgatc aaaatgatga ggatactgat aacccacgcc acggttgagt gatttcgcac
    18181 ctttgtaatg gctatctctc aaatgatcag gaacatctcc agctttgccc tctcgaatat
    18241 cagcaagtgc tgcatctaat gccatgtagg cagaattgga ttttggcgac aagcaaagat
    18301 cgacgacgac gtctgctagt gggatacgtg cctcaggcaa tcccaatttt tcagcagcta
    18361 atacagcgtt cactgtacga gccgcagcag caggattacc taaacctata tcttcataac
    18421 cgataaccat cagacgccgg caaatacttg ccagatcacc tgcctcaact aaacgggcga
    18481 gataatgaag tgcagcatcc acatcacttc cacgaatcga tttttgaaaa gccgaaataa
    18541 cgtcataatg agcatctcca tttttgtcat gggtaagagc ttttctttgg acacattctt
    18601 caatgatcga cagtgtaaga tgaatccttc cttctttatc tgaaggagtc gatagtgtag
    18661 ccagttccag cccattcaat gcactccgca aatcgccgtt cgttgcacgc gaaaggtgaa
    18721 gaagagcatc ttcgtctaac tggattgctt gttgtccaag gccgcgttct ttatccctta
    18781 aagcatgttc gactgccaac tggatgtctt gttctgttaa tggtttgact tcaaagatct
    18841 gtgtacggct gcggatcgct ggattgatgg taatataagg attctcagta gtcgctccga
    18901 tcaagatgat tcggccactt tctaaatgag gaagcagaaa atcctgtttt gttttatcta
    18961 atcggtgtac ttcatccaat aagaggatca ctgtaccact catttttgct tcttccgcta
    19021 caacttgtag atcctttttt gaatctgtag cggcgtttag catgcgaaat gcataattag
    19081 tagagccggc gatcgcactt gcgatactcg tttttccagt accaggaggt ccatatagaa
    19141 tcatggaaga gagcatgcga gcatcgacca tgcggcgtat gatttttcct tccccgacta
    19201 gatgctgctg accaacgact tcatcgatcg ttcgaggtct catgcgataa gccaatggct
    19261 gttgcatagg attttcctcc ttcttgtttt tacgtccctt aattatatca tatttagcaa
    19321 gatgcctaga aagaacagcc ttctgttgtt ttgttttcat agcttatcca actaatattg
    19381 tgtatgataa agtaggtgag aaaaatgacg gataaacaaa agatagatga atttttttgg
    19441 aataaaacga cgccagaagt ggcacgggat ttattgggga tgtatttgga gcatgacaca
    19501 ccagaaggaa gattagctgg atatattgta gacgcggaag catacctagg tccagaagac
    19561 gaggccgccc atagctatgg tttaagaaga acaccgcgtg tgcgggcaat gtatgaaaaa
    19621 ccaggtacga tctaccttta cacgatgcat actcatcgga ttttgaacat cattactcag
    19681 ccagaaggga ttcctcaagg agtaatgata cgtgcaatcg aacccgccgc aatgattgat
    19741 caaatgagta aaaatcgtgg cggaaaaact ggtccagata tcagcaacgg tccgggaaaa
    19801 ttagtggaag cattagcaat tcctcaagaa ctttatggtc agtctatagc agatagttcg
    19861 ttacgcttag tttttgagaa aaaaaagaca ccaaaaaaga tcaatgcttt gcctagaatc
    19921 ggtatcccaa acaaaggtgt atggacagaa aagccgttgc gttttgttgt gtcgggaaat
    19981 ccttatatct ctttacaaag aaaaaaccaa atcgagaaag attggggttg gaggaaagaa
    20041 aatgaaaaag aaggatctat taacatattt agatgaaata attgaaaaac aagtgacaga
    20101 ctatgatgta gctttagatt gggatacaaa aaatcatacg atcgagatcg ttgttcgctt
    20161 gtttgcagaa aataaagcac aatttgagat tgatgatgca gaagggatcg tttcgcaaga
    20221 accaattatc gaatttgaag acggcgtact tctttttaat cctcagaaat ctgtttttga
    20281 tgaacgcaat tatctagcag taatccctta tgaaggaaaa aaagggttgg caaaatcagt
    20341 agctgattct ttagtggaat atctaaatga agtattagct caagggcaaa gcgatttgct
    20401 tgattttttg gatgaagacg atgacgaagc tgtattcgag cttcactggg ataatcagca
    20461 actagcagaa ttagtagaag taaaacagca aaatgaagca gatacttatt taccgtaccc
    20521 aagttattaa ggagtgacag atatgaaatg gaatgaagta aaagtagaaa ctgaaagtga
    20581 agcagtcgaa gcagttgcta atattttgat ggaggcaggg gcgagtggtg tcgcgattga
    20641 agattcttta gatgttgaac attttcagtc ggatcaatat ggtgagattt tagataaaaa
    20701 tgattttaca tcgctgaaag aaggcgcact agtcatggct tattttccgg aaacgacttt
    20761 cttaccggaa atcttgcctt ttatcaaaga tcgtatcaca caattacctg agtttggtct
    20821 agctattgga aaaaataaag taacagttag tgaagtagag gaaagcaact gggcaacagc
    20881 ctggaagaaa tactatcatc cggtgcgtat tacgcgaatg ctgacggttg cgccaagctg
    20941 ggaaaactac cagacagaag atcctctaga aaaaattatc acactagatc ctggtatggc
    21001 atttggcaca ggcactcatc ccacgacttc cttgacattg acagcactag aaacagtctt
    21061 gcgtggtggt gaaacattgc tagatgtggg aactgggtcg ggcgtgctaa gtattgcagc
    21121 aaaatatctt ggtgcaaaag atgtctatgc ctttgattta gacgaagtag ccgttcgttc
    21181 ggcgaaagaa aatatggata tgaatgaaat agccaaagat gtccatgtat cagctaacga
    21241 tcttttgaca ggaatcgaga ttgaaagtga tgtaatcgtt gcgaacattt tagcagatat
    21301 catcttattg atgatcccag atgcttggcg tttactgaag caaacaggca cgctgatcgt
    21361 ttcaggaatc attgaagaga agaagcaaat ggttctggat gccatgttag aacaagggtt
    21421 tatcgtggat caaattttcc agcaaaaaga ctggtatgca attattttga aaaaaccaga
    21481 ggtggaatag atgcagcggt attttttaaa agaatcatat gaatcaaaag actattttgt
    21541 tttagaaggg gaaaactatc accacattgt acgggttatg aggatggaac ccgaggatga
    21601 agtttttttg gcattcaatg atcaaatagc cattgttgca gaaatcacgg agattactga
    21661 agaggctgtt tatctgaaag aaattaaaaa agaagaaaat caaaaggaac tgccaagttc
    21721 gattaccata gcgagtggct atcctaaagg ggaaaagctg gattggatcg ttcagaaatc
    21781 cactgaatta ggagcgcaag cttttcttgg tttcccggca tcagcctctg tcgttaaatg
    21841 ggatgcaaaa aaacgtatga aacgacaaca acggcttgaa aagattgctc aagaggcagc
    21901 ggaacaatcc catcggcaga tcgtgccatc agtagagtta ttggaaacga aacaagattt
    21961 atttgagcgg ctagatgatt atgacgtggt cttggttgct tatgaggaat cagcaaagca
    22021 aggcgaaaaa tcaaatgcag ccaaaattct tagtgaaaca aaaccagata gtcgaatctt
    22081 agctgtcttt ggaccagaag gtggattatc ccgcgatgaa gtcgattatt ttgtagaaca
    22141 caaagggata ctttgtggat taggaccaag aatccttcgt acagaaacag cacctcttta
    22201 tttattaagt gctgctagct atcaatggga attaatgaat ccttctttca gctaacgttc
    22261 ttcattaata aaagagtatc gacagaaaga attgaaaaaa tagtcaacaa tttgtataat
    22321 agaaacaatt atttatacaa tggatacgaa aaatcgtttg aaaaagcttt taaaaagcga
    22381 acatttccgt aaaaaaacgt ataactgcac ggattttggg acaattatca aacagatatt
    22441 tgtcccgatt cccgttttac cgaaaaaaga aagggggatc gattcctgtg acaaaagaag
    22501 aaattttaac gggacccggt gtcataaagc tcgttagtca atacatgggt cccgaacatg
    22561 tagcatttgt tgagaaagca tgcgaatacg cgactgctgc acatgacgga cagtttagaa
    22621 aatcaggcga accctacatt attcatccta tccaagtcgc aggtatatta gcagatttaa
    22681 aaatggatcc ccatacagtg gctacaggct tcttacatga tgttgttgaa gatacagaaa
    22741 tcactttaga agatctgaga gaagaatttg gcgatgacgt tgctatgtta gtggacggcg
    22801 taaccaaatt agggaaaata aaatataaat cccacgaaga gcagctagca gaaaaccacc
    22861 gaaagatgct gctagcaatg gctcaagatt tacgagttat catggtcaaa ttagctgata
    22921 gactacataa catgcgtacg ttgaagcacc tgcgagaaga taagcaaaga aggatcgctc
    22981 aggaaacttt agaaatctat gcgcctcttg cacatcgtct agggatcagc cggatcaaat
    23041 gggaattaga agatacagca cttcgttatc taaatccaaa acagtattac cgtatcgtcc
    23101 acttgatgca gacgaagaga gaagaacgtg aaaaatacgt aagtggtact gttgaagata
    23161 ttcgaatagc gacagaagag ctggggattt ttgcagaaat ctatggacgg ccaaaacaca
    23221 tttattcaat ctatcgtaaa atgaaagatc agaaaaagca gttcaacgaa atttatgact
    23281 tgctagctat tcgagtgatc gtagattcaa tcaaggattg ttatgctgta ttaggagcaa
    23341 tccacacaaa atggaaacca atgcccggca gatttaagga ttatattgcc atgccaaaag
    23401 ctaatatgta ccaatctttg catactaccg tgatcggtcc ggcaggaaat ccggtagaaa
    23461 ttcaaatccg aacacaggaa atgcatgaaa tcgctgaatt cggggttgct gcacactggg
    23521 cctataaaga aggaaaaaat gaaaaagtag aaccagatgg tatgacgaaa caattaagct
    23581 ggttccatga gatactcgaa cttcaagacg aaagctatga tgcttctgaa tttatggaag
    23641 gcgtaaaagg agatatcttt agtgataaag tctacgtctt cacaccaaaa ggagacgtta
    23701 ctgagttacc aaaaggatcc ggaccattgg actttgcata cagtatccat acagatatcg
    23761 gtaacaaaac cactggtgca aaagtaaatg gcaaaatggt gcagcttgat tacaaattga
    23821 aaaacggaga tatcattgag atcatgactt ctccaaattc atttggccca agtcgcgact
    23881 ggttgaaatt agttgctact agcaaagcaa gaaataagat caaacgtttc ttcaaagccc
    23941 aagatcgaga agaaaatgtg atcaaaggcc acgaatccgt ggtcaaatgt attacagatc
    24001 taggatttac gcctaaagat attttgacga agaacaaact gcaagaagca ctcgatcgtt
    24061 ttaattatca aacagaagat gatctctatg cagctgtagg gtatggagaa gttagcccct
    24121 tgacgatggc caatcgtctg actgaaaaag aacgtaaaga acaaaaaatc gagcagcaaa
    24181 agcaagaagc agaagaaatc atgaatcagc cgaaaaaaga acctgacaaa atgaaagtac
    24241 gtcatgaagg tggcgttgtc attcaagggg tagaaaactt actaattcgt atcagtcgct
    24301 gctgtaatcc gattcctggt gatgatatcg ttggttatat cactaaaggc agagggatat
    24361 ccattcatcg tcgagattgt ccgaatgttc agcctgacaa accaaatgta gcagaacgtt
    24421 tgattgaagt cgaatgggaa gatacatcga atacacgaaa agagtatgat gcagatttgg
    24481 aaatttacgg ctataatcgt tcaggcttat tgaatgatgt acttcaaaca gtcaatgcgc
    24541 taacgaaaaa tctcaacagc gttgaagcac ggacgaataa agataaaatg gcgacgatcc
    24601 atttgacggt tggtatccag aatttatccc acctaaagag tatcgtggat aaaatcaaag
    24661 cagtacctga tgtctacagt gtacgccgga cgaatggata ggaggacaga tatgagagca
    24721 gttgttcaac gggtaagtaa agctgctgtg tcagtcgatc agcaagaaat tggaaaaatt
    24781 ggctacggcc tacttgtttt attaggcatt catgaaaccg atacgcaaaa agatgtggac
    24841 tatctggtaa aaaaaattgc acaaatgcgt atttttgaag atgaacaagg aaaaatgaat
    24901 attagtgtag aagatgtggg aggacaaatt ttaagtatct ctcaattcac actgtttgcg
    24961 gatacgaaaa aaggaaatcg accaagcttt gtggaagccg cacgaccaga aacagctatt
    25021 cctttatacg aatcattcaa tgaaggaatc agaagtcgcg gcatcacagt cgctaccgga
    25081 caattcggag cagatatgtc ggtttctctc gtcaatgatg gacctgtaac gatcattatt
    25141 gattctaaaa acaaataaag taatctggtt ttcactagta aaaactatga aacacgtcgc
    25201 ttaagttaga ctgataaact ctaacttaag tggctttttt gtctgcaaaa agcgcttctt
    25261 ttgtcagcag tcggaaaaaa agttaagctt aaattataca agttattttt tataggaggt
    25321 tgctgaagat gaaagaagta ccagtagtag atatcaaaat cacagattta aaaaaattgt
    25381 atcaaaaagg atacaacatg ttggaggagt tacgccatga agcggacgct cccgtcgtca
    25441 aagcaaagat tttcaataaa gaagctatca cgatctacgg atcatcagca gcaaaagtat
    25501 tttatgatcc aaggaatttc aaacgaaaag gtgctatgcc aaaattagta ttgaagacac
    25561 tttttggtca aggtggagta cagactttgg atggagctgc tcatcaccat cggaaaaata
    25621 tttttatgga tctaatgaca ccggaaagaa tggaagatta tcatcgtatc ttagataaga
    25681 atctgacaca agcacttgaa gcacagcatg gtcaatttga actatttgat ttaagtaaga
    25741 tggttttctt tacatctatt tgcgagtggg caggtatcaa tttatcagcg atttctaaag
    25801 atgaggtcga aaaattagca gaatatcaaa tctctatgat cagcggaacg tttacttctc
    25861 cgattgatca cataaaaggt gtcgaaaatc gcaaaaaatc agaaaaatgg gcgcaaggct
    25921 tgattgaaga agctcgacaa aatcctgtgg ctggaaaaga aaatgtggca ttatatgctt
    25981 ttgccaatgc cacagattta gatggtcagc ttttacctct ggaagtggca gctgttgaat
    26041 tattgaacat catccgtcca acggttgctc taactgtgtg ggcggcattg atgggacatg
    26101 cacttttcag tagaccagat ttgtatcagc aattaaaaaa tgacttttcg acgttacaag
    26161 atccgtttat tcaagaaatg agacgctatt atcctttctt cccaatgcta cctgcgattt
    26221 cgctgaaaga agtagaagta gatggttatc gtataccaga aggcagttgg gttattttag
    26281 atttatatgg aacagaccat gatgaacgaa cagttgaagc gccagattcc tttatgatca
    26341 aacggtatgt agggaaagca aaagatattt cctataaaga agaatatgag atgattgcac
    26401 aaggcggggg aaattttcga caaatgcatc gctgtgcagg agaatggatc actcttcata
    26461 gtctacgtgt gttttccgat cagctagtaa ataagtttga gttctctgta cctgaacaag
    26521 attggacgat tccattcaat cagtttccga cttatccgaa tagccgagcg ctactttaca
    26581 agaattagat tagcataaac ttatcgaaaa aaaggaagat caaacagtgc tgatcttcct
    26641 tttttcatat tactataaat tgataaggtc aattgatcaa aatccacgaa atgcttcatc
    26701 aatggtttga taatcttcat tggataaatg caaatctaaa gcttggacat tgtcagatac
    26761 ttgctctgga cgcttagctc caggtataac tacagacact cgtggattct tcatgtacca
    26821 agctaaaaca agttgcgcag gagtggcttg gtatctttta gcaatggggc gaagtacatc
    26881 gacagcggtt aaaatttctc tgaaacgttc tccttggaaa tctggatttc tttttcgagg
    26941 atctccttca ccaaattgtt tttcctcacc aagttcatat tttcctgtca gcaaaccgga
    27001 agcaagagga aaatagggaa caaaactgat tttgtttttt tcaagataag ggaataattc
    27061 tttttcagcc tgccgatgga tcaaactgta tttatcttct actacatcga catatccatc
    27121 tgcattcgct tctttcaatt gttctaacgt gaagtttgac acaccgactg ctcggatttt
    27181 tcctgcttct tttaattcgt gcaatgtagc aactgattca ttttttggtg tgctttcatc
    27241 cggaaagtgg atataaaaaa tgtcgatgta atcagtttgc agacgtttta gtgcatcttc
    27301 caccgattgt ttgagaaact caggagaatt atcaaatgtt cgccctttat ttggtacatg
    27361 agcagctttt gtagcgatga cgacacgtga gcgatcatat tctttttcct tcagtacctc
    27421 gccaatcagt tcttcggaac ggccgtttcc gtaagcataa gcggtatcga tcaaattgat
    27481 cccgctattc aaagctgtac gaactacttg ttttcctgtc tcatcttcca aaccgtcaaa
    27541 aagattatgt cctccaacgg catttgctcc taatccaagc tgttctgcgt agacttgtga
    27601 atgtccgatt cttatttctg ccatagctga ttcctccttt ttgtctagtc taaaacaaaa
    27661 taccaaaaag ctgaactaaa acgtttttgt gaggggaaat agtcagatat ctcttgactt
    27721 tgagactggt ttttagtagg ttataagtaa tatcgaatgt caatgaaaga gcgagtaagt
    27781 ttttgaaact ttacaagaga ggatcatcga ggctgaaaat gatctaaagt ttcattagcc
    27841 taaagacact ctggagatgg acgagtgaaa agccgtcccg cgtacgagcg ttattcgtaa
    27901 gaggaaagag aaatctttca aaattaggtg gtaccgtgta tgaatttcat tcacccttgt
    27961 tcatgatgaa caagggtgat ttttttattt acatagctaa gaaagggaga gaaaactatg
    28021 agtttccaaa gaccaaaagg aacaaatgat attttgccag gcgaatcaga aaaatggcag
    28081 tttgtcgaag aaacagcacg tttattattc agagattatc agtataatga aatgcgtaca
    28141 ccgatttttg aacactatga agtgatatcc cgaagtgtag gggatacaac ggatatcgtt
    28201 tcaaaagaga tgtatgattt ttatgacaaa ggggaacgcc atgtgacgct tcgaccagaa
    28261 ggtacagcac caatcgttcg ttcttttgtt gagcataaat tgttcggccc agagtatgcg
    28321 aaaccgttca aaacttttta tatgggacca atgtttcgct acgaacgccc tcaaaaagga
    28381 cggttacgtc aattccatca aatcggagta gaagcatttg gatcagaaaa ccctgcaact
    28441 gatgtggaga gcatggtgat ggcacttgat ttcttcaaac agttggggat cacgcaaatc
    28501 cggctagtca tcaattcatt aggtgataaa gaaacgcgtc aagcctatcg tcaagcattg
    28561 atcgactatc ttttaccttt tgaatcagag ctgagtgaag actctaaacg ccgccttcat
    28621 gaaaatccat tacgcgtatt ggacagtaaa gacaaacgag atcaaaaatt tgtggcagaa
    28681 gctccatcta tcttagatta tctaagcgag cctgctcaaa aacattttga taccgttgtt
    28741 tccatgctgg atgcattggc tgttccttat gaaatcgaca gtaatatggt tcgcggacta
    28801 gattattaca cacatacgat ttttgaaatc atgagtgacg caccaaaaat gggggcacaa
    28861 gcaacgattt gcgcaggcgg acgttatgac aatctagtgg aagaactagg tggtccttca
    28921 acacctggct tcggatttgc aatgggtatc gagcgattac tcttgactat ggaggcagag
    28981 gaagtagtta tccctgcatt taatgagttg gatgcttacg tggtcgcact cggagacgaa
    29041 acaaatatcg aagcattgaa agtggtccaa gcgattcgaa actttggttt ttcagctgac
    29101 cgcgacttca tgaaccgtaa agcaaaagcg caatttaaga cagcagataa agcgaatgcc
    29161 aaattagtat tggtgatcgg tgaagatgag cttgctagtc aaacgattaa tgtcaaagca
    29221 atggcaagcc gaaaagaaaa aagttttcca ttagaagaga tatatgaaca ttttgatgaa
    29281 gtctatgatg aaatgacttt attcgagtag gaggaaaaaa ataaatggca caaagaacga
    29341 tctattgcgg attagtttcc gaacaacaat tagaacaaac agttacatta aaaggatggg
    29401 tacaaaaaag acgagacctt gggggagtaa tctttatcga cctacgtgat cgtgaaggaa
    29461 tcgttcaaat cgtctttaat cctgaaatca acaaagaagc atgggaaatc gcggataaat
    29521 gtcgcagtga atacgtgatc gaagtgactg gggaagttag aagacgcgga gaaaatgcag
    29581 taaatcctaa aatggccact ggtgaattcg aagtgattgc aagtgaaatc gtgattttga
    29641 atcaagcaaa aaccccgcca tttcaaattg aagatgatca agcaatcagt gacgaaatac
    29701 gtttgaaata tcgttatttg gatctgcgtc gtccaaaaat gactaaaaac atcaaattaa
    29761 gaaatgcgac aacaaaagcg attcgtcatt atttagatga taatgacttt ctcgatatcg
    29821 aaacacctta cctagggaaa tctacaccag aaggcgcaag agactaccta gttccttcac
    29881 gtgtccatgc tggacatttc tatgcgttgc cgcaatctcc tcaattgttt aaacaattgc
    29941 tgatgaatgc tggattcgac cgttattatc aaatcgttcg ttgtttccgc gatgaagatt
    30001 tacgtggaga ccgtcagcct gaatttacgc aagtagatat tgaaacaacg ttcttaacag
    30061 cagaagaaat ccaaacttac acagagggat tgattgcgaa ggtaatgaaa gaagttcgcg
    30121 gtatcgaagt cacattgcca tttcctcgta tgacctatga tgaagcaatg gctcgctacg
    30181 gcagcgacaa accggataca cgttttgata tggaattgat cgatctaagc gatacagtca
    30241 aagaagtaga attcaaagtc ttccaaatgg ctcttgaaaa cggcggtgtc gtgaaagcat
    30301 tgaatgctaa aggcgctgca gatcgctact cacgaaaaga catggatcag ttaggtcaat
    30361 atgtaggaca attcggagct aaggggcttg catggctgaa agtagaagaa gacggattga
    30421 aaggtccaat tgctaaattc atgggagaag caacagaagc aatcattaaa gcaacagatg
    30481 caaaaccagg tgacttgctg atgtttggcg cagataaaag tgaaatcgtc gctgctgcat
    30541 taggtgctat ccggacacgt ttaggaaaag aattaggact aattgatgaa tcgaaattca
    30601 atttcttatg ggtaactgat tggccacaat ttgaattttc agaagaagaa ggtcgatatg
    30661 tctctgctca ccatccattc acgatgccaa aagaagaaga tatcccgtta ttggctgaag
    30721 accctgcaaa agtctatgca gaagcttatg atatcgtctt gaacggttat gaactaggcg
    30781 gaggttctat ccgtattcat actcgtgagc ttcaagaaaa agtactagaa actctaggct
    30841 ttacaaaaga acaaatggaa gaacaatttg gtttcttgtt aacagcttta gattacggat
    30901 tccctccaca tggcggtatt gcattaggat tggaccgttt tgcaatgtta ttagccggag
    30961 aagataatat tcgtgaagtc attgcctttc ctaaaaacgg aaaagcagca gatgtgatga
    31021 gtgatgcacc aagtatggtt tcgcctttac aattgttcga attgaatatt gatgtaacga
    31081 atatagaaga atagccgata aaaaggtaaa aaggatttgt cgaatacgct tcttttgaca
    31141 accttgattt aattgaaaaa cactttataa tgaagccata acatataaag cggggctttt
    31201 ccatgaaaaa aacattcgaa aattgggctt atcatgatta cacgaccaag ctttcggtat
    31261 ctatcgttta tgcgttgatc gcatcagtcg cattgaattt cttttatcag ccaggacata
    31321 tttattcaag tggtattact ggtctagctc agattttgac gactttgtca aaatcactga
    31381 taggattcga agtaccggta tcaacaactt tgtatttact gaatatacct ttgtttgtat
    31441 tggcgtggtt aaaaatcggg aagaagttta caattttcac ttttatcaca gtcactttga
    31501 cctccctttg tatccatatt ttaccggaaa cagtcctatc taccgatcca atcatctgtg
    31561 cgttgtttgg gggggctgtg atgggactag gtattgggct agcgttgaga aacggcctat
    31621 catcgggcgg tctagatatc gtgagtatca cgattcgtaa aaaaacagga aagtcgattg
    31681 gttctatttc gatctacttc aatgcattga tcgtttttgt tgccggaatt ctatttggct
    31741 ggcaatatat gttttacagt acgttgtcta ttttcgtcag tggaaaagta actgatgtcg
    31801 tttatactaa acaaaagaag atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag
    31861 taaatgaaat ccaaaactgt atgcgtagag ggatcacgat catcaatgaa gcagaaggtg
    31921 cttataagca tgataagcag acagtattga tgacgatcgt tacacgatac gaactgcctc
    31981 tacttagaga agcaatgaaa gcttctgatc cacaagcttt tgtaagtatt gcagaaaacg
    32041 tccagatact tggtcgtttc tatgaggatg gcatttagat cgagtttgac aaaaattcat
    32101 gaaatactgc tgtagacaga cgttcgtttg tctgcagttt ttttctacca tagtagaatc
    32161 agcaaagaaa aactgctttt ttatttgaaa ttttcttaaa gttgaaaaaa agcatgttta
    32221 tttttttaaa tttatgggat gatagaaaaa aatagtagag aaaggacacg attctacttg
    32281 ctattttcac ttaatacccg tatattacga atgaaaaaag tttatgaaaa gttggaatat
    32341 tgaatggaaa aacaaaaaag gttaaaaaac agccgctaca tcactgggtt tgatgggctc
    32401 cgttcattag ctgtggtggg tgttatcttc tatcaccttc tgccaactag tatgaaaggc
    32461 ggctatttgg gcgttccgat ttttttcgtc gtatctggtt atttgatcac ggatctgctg
    32521 cggcaggaat gggaacaaaa tggaaaaatc aatatttggc aattttatgt cagaagaatg
    32581 aagagactct atccggcact tgtttttatg cttgtgacag cttcggctta tatcactttg
    32641 tttcagcgag ggctgttaaa caatcttcga ggagttgtgg tcagcagctt actgtatgtc
    32701 aacaactggt ggcaaatcaa taacggttta tcttatttcg atcgatttgc taatgagtca
    32761 ccgtttacgc atatctggtc tttagcagta gaaggtcaaa actatttggt ttggccgatc
    32821 ttgtttgtat tgttgatggt atttgttcgt aaaagaaaat ggatctttta tactgtgtta
    32881 ggtacatcct tcctctccgc catcttgatg atgatcttgt atacaccagg aggcgatcca
    32941 acccgtgttt attacggtac cgacactcgt ctgttttcta tatggttggg aagtgcgctt
    33001 gcgtttgttt ggccaagtac gcgtttaaag aaaaacatcc cgaaacaagc gaaaaatatt
    33061 ctgaatatcg ctggaggaat ctcgcttttg gcactgattc tttgcttctt ctttttagat
    33121 gatcattaca atttcatcta ttacggagga atgctcctag tgagtctgtt atgcgtagtg
    33181 ctagttgcag tgacagcaca ccccggagca agtttgaatc gatggctgac aaatccagta
    33241 tttacttgga ttggcaaacg aagctatggc atttatctgt atcagtttcc agttatgatt
    33301 ttttatgaag caaaggtgac tgatattgcc gatcatgttt tgttgcatac tttagtggaa
    33361 atcacattga ttctaatcat cagtgaactt tcttatcgat atgtggagcg tccgctagca
    33421 cgttttgatt atggtcgtac attccaagtg atgaaaggat ggttcagcaa accgatcatc
    33481 agtaagagca aaccatgggt tgttccaggc acattacttg tagcaattgc actcgttggt
    33541 ttcgttacgg caccgaaaaa cgctgtaaca gccgatcaaa aacaactgca ggcaaaaatc
    33601 gctgaaagta aaaaattagc agaagaaacc agaaacaatt cagaaaacac cactagttcg
    33661 gttgaccaag cgatccttga taagtacgag ttgtccaagg cacaagggaa taaggcacaa
    33721 aaattagaac taacggcatt tggtgattca gttatgttgg atgctgcaac ggatctacaa
    33781 gaactttatc caaaagtagt ggtagatgga gatgtcggac gacaattata tgccagtgta
    33841 ccgtacattg aaaaattaaa ggaacaagat ttgttgaaag acactgttct gatcggacta
    33901 ggaacgaatg gctcattctc ggaagctcag tttgatagtg tgatggatgc aatcggtgat
    33961 cgaaaagtat actgggtcaa tgtccgagta ccgacgaagc gctggcaaaa tgacgtgaat
    34021 gcgatgcttg aacagatgac gaaaaaatac gacaatatga cgctgattga ttggtacagc
    34081 tacagtaatg accatagtga ctggttttat gatgatcaag tccatccaaa tcctgaaggg
    34141 atgacacatt acattcactt agtcagcgaa gccttgctag gaggaagttc aagtacgaac
    34201 agcagtgaga caaacgtgac agaatgatag ctacaaaaaa cttcaggcaa aaacatttgt
    34261 ttttgcctga agttttttta tatttaccgt gtttttaaca ttcatataga ttcaggaagc
    34321 aaaacggttg agtgtgtctg taaataacgt tctttttgtt ttcgggtgag ttttttgaat
    34381 gcagcttctg cttttgttgc ttcgcttctt gaagaaaata cttctgcatg gatcatctta
    34441 acaggtcttc ttttttgaag acgagtatat ttagcaccag taccattgtt atgttcctct
    34501 aatcgtctag aaagatcggt tgtatatcca ccataaaatg tctgatcttt acagaataat
    34561 acgtaaaaat aatgttcatt tgccatagag catctcacga atctctgcag tgtattctcc
    34621 atccatattg taagtgatca atggtggagc gactttgaat ccgtctaatt tgccttcttt
    34681 gattccttca actaacaaaa tatttgcctc ttttccaggt tttgggtaaa caaattggat
    34741 ccttttaggt gcaattcgat agtcttccat gacctgaatg atttctaaga accgatcagg
    34801 acgatggacc atagtaaagc gtccatttgt ttttagtagc ttacttgaaa tatcgatcac
    34861 ttcttctaac gtcgtatgaa tctcatgacg ggcgattgcc aggtgtttat tagggttcgt
    34921 tttatttgtt ggcagccctt tgaaataagg tggattacag acgactaagt cacaagaatt
    34981 atgtcgaacg acagataagc tgtctttcag atcgatggtg tggacagtca tttgttcatt
    35041 taaatggtta agcgcgatac tacgtcttgc catgtctgct agacgaggtt gaagttcgat
    35101 agcgtcgata gccgcctggg ttcgtttact taaaaaaagt ccaactgcgc catttcctgc
    35161 acaaaggtca acaatcttcc cttttttagg tacacgggaa aagtgagcaa gcagcacagc
    35221 atctaaagaa aaagaaaaca ctttggaact ttggataatc ttgatatctt ctgcgtataa
    35281 ttggtcgatt cgttcgtctt cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg
    35341 tctgtattat actattttta ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt
    35401 atgcatacta tgaccataag aagggaaagg aagagactat gttttatcga tttatgcgcg
    35461 gggtagttcg agtactatta gccttagtta atggaaatgc ccgctatgag aataaagaag
    35521 ttttaccgca agatgagaat tacatactag ttgctcccca tcgtacttgg tgggacccat
    35581 tatatctggc ggtagctgca gcacccaagg aattcagctt tatggcaaaa gaagaactgt
    35641 tcaaaaatcc agttctgcga tttatattaa ctcgatccaa tgcttttcca gttaaaagag
    35701 aaaatccagg accaagcgtc attaaaacac ctgtaaagat tttgaaatcg acaaatttga
    35761 gtttgatcat gtttcccagt gggacaagac atgctacaga acttaaaggc ggaatggcat
    35821 tgatttccag aatgtctaaa gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac
    35881 ttggggatct gttcaagcgt cagagagtga cagtgcgttt cggcgaacca atcgacttat
    35941 cagatatcaa gaaaacggat aaagaaggta tggcggagat cgaacgacga acacaagcag
    36001 cttttgacaa attggacaaa gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat
    36061 aaaatgataa gtttatgcgt gggcggtctt ttgaatctac tgtcctgttc agttcagact
    36121 ttttcaatga aaacgaggga gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta
    36181 aaatagaaga caggtgtcat gtaatggatc aaagtgttat aataattaga aggacatttt
    36241 ttgaggtgat tgaatgcgtt ttttacatac agccgattgg catatcggaa aaaaactaca
    36301 aggttatgac ttgattgaag accagcgtca tgtactatct tctatactag atatcgcgaa
    36361 agaagaaaaa gtagaggcaa tcgtgattgc cggagactta tatgatagga gtgtcccagc
    36421 tgttgatgcc gtagaattat acaatgaatt gatggtcgat tggaatttaa aggaaaagat
    36481 cccgattttt gctatatcag gaaatcacga tagcagtacg agactttcag cgggtacgcc
    36541 atggttcagt tattcgaact attttttgcg aacacagcta gcagaagcat ttgaaccagt
    36601 cgagtttaaa aacgtacaat ttttctttct tccttacttt gaaccaattg cagcgagatt
    36661 atattttgaa gatgacaata tccgaacgat caagcaagcg gtagccaagg tgatagataa
    36721 aatgcaggag ctatttgatt cagaaaaaaa acaagtattg attagccatt tttttgtaat
    36781 gggaagtgta aaatctgatt cggaaacgaa aatcgaagta ggcgggttgg attcgatccc
    36841 tggtgaacta cttacaccgt ttgattatgt agcactagga catttgcata atcaagctgc
    36901 tttgagacag gataatgctc gctatagcgg ttccccattg aaattctctt tatctgagat
    36961 caaccaaaaa aaaggcgtat ggatcgttga cctgacaaca ggcctggagt tgaattttag
    37021 ggagatcgaa cccctgcatg atattgaaga aataacagcg agtttcaaag aattgctgga
    37081 tcctgccttt tatcagtccg tcaatcggga gaattacatc tatatctatc tcactgatag
    37141 aacgatcatc ccgaatatga tgaaccagct tcggcaaata tatcctcgta ttttggggat
    37201 tgaacgggca aacggtcgag cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa
    37261 agatcctcaa aaattagcag aagcattttt tagtgaaatg acggaagagc agctaactgc
    37321 aaaccaagaa aaatggctcg gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag
    37381 aatgaaaccg aaaaaattac aacttaaaaa ttttggtcct tttatcaatg agaccgtaga
    37441 tttttcacgt ttgacagaag caccgctttt tttgatcagc gggaagacag gagctggcaa
    37501 aacgacgatt tttgatggga tcaccttcgc actttttggt gaaacttccg gaagacttcg
    37561 ctctggaaaa gagatgcgtt ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt
    37621 ttcttttgaa catcagcaga tgagctatga aatcgaacga aaaccagaac aagttttagc
    37681 aaaacgaaaa ggaaacggaa caaaaaaaca ggcagccaaa gtaacgttga ccatttttga
    37741 tgggaaagga aaagaaataa aacaaatcac aaaacgtaca gaagcagatc agttgatcag
    37801 agaactgatg aatctcgatg cgaaacagtt ttctcaaatc gttttactac cccaaggaga
    37861 attccgtaat tttttggttt catcaagcag tgataaagag actgttttac gtcatttatt
    37921 cggtactcaa tttttccaac agttcaatga gcgattaaaa gaaaaagcaa aacaacagca
    37981 acaaaaattg gatcatttag agcaagagct gcatcttctg caaaaaaggt ttgttttagt
    38041 caaagaagag gaaccagcag cagggtttga agccgtttta gcccaatgga caaaccatca
    38101 ggcaatactc gaaaaacaaa tcacggaaga aaaagaagct ctaacggaac ttcaggaaaa
    38161 acaaaagcaa ctagaaaatt cctactatcg ttttgaaagc ttgaaaaaaa gttatgaaga
    38221 aaaaacgata ttaataaaca agcaaaagga attagctgct caaaaagacg aaatagagga
    38281 aaagaaacga tggattgggt attatgaatt tacgcaacga ttagtcgaac caatccagcg
    38341 cctacaagaa ataaaagttg aaaaaataga actgtcacaa aaaagtagtg agcaaacaaa
    38401 gaaactagcg atgactacgc agaattatga agagtggcaa aaaaatgaag caaaccgcgt
    38461 acgccgacaa acaaaaatca aagaagctgc tcaaaaactg caaaacaaac agatgatggt
    38521 gccaattgtc gaatccttac gtagtaaaaa acaagacgca gaacaattaa caatttcatt
    38581 ggataaagaa gaattgaatg ccgagaagct gtatcaaaat ctcgctatct atcaggaaag
    38641 aactcgaatt cttcaagaaa atttactgga tcaggaaaaa ctgcaagaag atcgtcttgc
    38701 ttttggacag ttaaaatatc tagatgaaaa gcagcaagaa atagaaaaac aaaaagcaaa
    38761 agacactggg tcgctcgcac agataagaga agaactcaaa aaagcaactg aagaaataac
    38821 aaaaaatagt cagctattga aagaacagac agaacaagca gaacagctga aaagccaatg
    38881 ggcaaagcag caaattgctc gtcttcagct ttttttgcgt ccaggggagc catgtcttgt
    38941 ttgtggatca acggaacacc ccatacaaca attaaaacac gaacagccaa ataaggaaga
    39001 tatcttaaaa gtagaacaac aactggcaga agctgagtct gctgtagaaa gaactcagca
    39061 aatcattgcc aaaagtgaag cacatgaaaa taatcttcaa caacaattat cagatttaga
    39121 acaaaaaatc atggcaacga atatacaatt ggctgaacaa gaagaacact tgcaacaggc
    39181 tttgaaaaag gcatttcctg agataacaga acaaaaaaca aaaacaactc ttgtagtaat
    39241 cgaacaatct ttagatagac gaaaaagcaa gatggccaat gcaagaatag agctggaaga
    39301 aataagacaa caagcagaaa agctcgaaca acaattagct gtctacaaac agcagcagac
    39361 agaaaaaaat aatcagctcc agcaactgca aggagaaatc aaaagtttag aacaacaatt
    39421 gcaagatacg gatagtaact gtttagaaga ggagatacga cgacttgaaa aacagttaga
    39481 agaagactcg aatcaactag ctgttgagca gaaagcagga gtgcaattgt tgcaagatct
    39541 tcatacgctt cggacacagc ttgatcttca acatgaacaa atagaaaaaa tagatgaaaa
    39601 aatagaaaag gaacaagaaa gaatagctaa aaagttagaa agccagtcgt atttttcaac
    39661 aattgaagaa atctgtgcat ttgcacaaca gcaagcacaa tataaagaaa accaacgtac
    39721 agttacttct tatcatgaag agcaattagt gacagctgac cggctgcgtc aattagaaaa
    39781 tatccagcta actgagacat tcccagatac agaagagatc agccaacagt tagaagaaat
    39841 aagacgtatg atcgaaataa agcaagaaaa gatatacggt ttacaagaac aaaaaattag
    39901 taatcaaaaa atctacgatg aatgtctttc gatttatcac acaagccaga cgaaattaga
    39961 tgagcttacg caattacagc agctttcgca aacattcaac ggagaaaatc ctaagaagac
    40021 tagtttggaa cggtatgtgc ttcaggttta tcttcaagaa gtgttacaag tagcaaatga
    40081 tcatctccaa cgtttaacga aaaatcgtta tcaatttgag ctagcggata ccattggcag
    40141 ttatcgaggg aaaaccggat tagaaatcaa tatctatgat gatcaggcag gtatgtctcg
    40201 aagtgcgcat accctgtctg gcggtgagag ttttattgca gcactttctt tagcattagc
    40261 tcttgccgat gtgatccaga cacaagctgg tggtgtagca atcgatgcat tgtttattga
    40321 tgaaggattt gggtcattag atgaagaagc tttggaaatg gcaatggaag ctttagaaac
    40381 gatcgaaagt gaaggaagaa tgatcggcat cattagtcat gtaagagaat tgaaagaacg
    40441 agtattacaa caaataagaa ttgatacaga aggtagcgga caaagcaaaa tacgctatca
    40501 acttggataa aaagggagat tggtaactat gaaatggagt attccagaaa aagtcattgc
    40561 acgtggacgt caatacttgc aagaaggccg agtgttatcg gttgtccctg accaagaaga
    40621 aaacgtgtgg catgcagaag tcttgggcag tgaattgtat atggttgatt tggatggtac
    40681 agcaaaagag atagattact gccaatgtcc ctattgggag gaacataaat attgtaagca
    40741 tacagttgct gtggaacttt atttacggtc gaaaaatcta tctcgtatca tgaaaaaaga
    40801 ccagccaatg ctagaaccgg taaaagcaac aagtgaggga gaaatcattg caaaaggttt
    40861 ttctcgtcta aaaacacaaa acaatcctca agcggtgcaa cctttgatta ttgaatacca
    40921 agtagaaacg attgagacta atcagtatca cccagagctt tctattttgg cactttactt
    40981 gcgtatcggt tcattggcat caccgaaaaa aacatatatc gtaaaaaaca tctatgattt
    41041 tttacaagct tacacagaaa aagaaactta cactgttaat aaacagtacc agtttcgtct
    41101 agataaacgg gcgtttcaaa aagaagatca aaaaatactc acgcatcttg caggaagtgc
    41161 tcagacgcat caattattag gtacgaacgg cttacaagtc aaaggaaaac tagataaaag
    41221 atatctgctt ctcccagtag aacaagcccg cttcctgtta gagcagatga atcagactac
    41281 acgtttgcat ctgcagataa atgaacaaaa cgatcaagga atacaatttt ccgatcaaaa
    41341 caaaccttta tcattcaaag tggaaaaatt tggagaagat tatttgctgc aagcattgaa
    41401 tgattttgat tttttcttta tgcattatca gtggggagtt atccaaggga cattttattc
    41461 tctgacgaaa tatcaacaaa cggtttatca aacttggaaa caattgattc gccgattgga
    41521 gaaaccagaa gtagtgttca agaaaaaaga attaccgtct ttgtttaaag agattatccc
    41581 tctgctgtca gagatcggcg acgtagctat tgcccctgca gtagaagagg aagtagcaga
    41641 tatccccgtc gaatttattt tctttttccg aaaagcaaaa ggaaaaatcc aagctcgagt
    41701 cgatttcgtc tatggcgaag tcatctattc gactgacccg aagcatgagg tctcgacaga
    41761 acaggctttt caggtgttac gtgacacagt agaagagaaa cgagtacttg atctattcag
    41821 aatgtatcgc taccagaaaa atgaaactgg ttatgaacgt ccattgccaa gcggggaaga
    41881 actatatgcc tttttccgca cagaactatc tgtcttccgt cagtttggtg aagtcagact
    41941 tggaagaaaa ttgcgagagt tgtatttaga tgcgcataaa catcaaccga aaatcgaagt
    42001 aaccgagacc ggttcatggc tcgatattcg attcgatgtg acagggatag aggagcaaga
    42061 aatcgatcat gtcctgcaaa gcttgctgag aaatgatgct ttttatacat tagaaaatgg
    42121 tcaggttctt tcttttgatt cagaagaatt tcaacaaaca agccaagtgt tgcaacaatt
    42181 acgggaatcg atacgtacag aagaaggaac gatccatgta cctaaaaatc aaggattgat
    42241 cattcaaaat caattggaaa aaagcaatgc tacttttagt gaatcgttcc aaacgatggt
    42301 acaagatctc attcatccag aaagatacca ggcacagctt cccaaaggat tgaatgcaac
    42361 gatgagagat tatcaaaagc aaggttttcg atggttgaag atgttaggtc attatcagtt
    42421 tggcggtatt ttagccgatg aaatgggact tgggaaaaca ctacagacaa ttgccttttt
    42481 actttcggaa aaagaagaac ggaaaagctt ctctgctttg atcgtagcgc ctgcgagctt
    42541 aatttacaac tggcaggcag aggtgagaaa attcgcccct tctttaagta tccaagtaat
    42601 caatgggaat aaaaaagaac gtgaagaatt actggctaaa gacacagata tccgagtgac
    42661 ttcttatgcc agcttaagac aggatctagc taattatcag tcgcaaaaaa ttgattattt
    42721 gattcttgat gaggcacaaa tggtcaaaaa tagcagtacg aaaactgcac aagcattgag
    42781 agaattagct gtacctcagc ggtttgcact gagtgggaca ccaatcgaga ataatttaga
    42841 agaattgtgg tcattgtttg caacgattat gccaggattt ttcccgacta aaacaaaatt
    42901 cagggaactt gccgcagaag aaattgcgca aatgattcga ccgtttattt tacggcgaga
    42961 taaacaaact gtgttgaaag acttgccgga aaaaacagag atgaatctat attctgcact
    43021 tacagaagaa caaaaaactg tttatttagc ttacttgcgt caaatgcgtg aagaaatcac
    43081 atcaatggat tctgaagcat tcaagaaaaa ccggatcggg atacttgctg gtctgacaag
    43141 actgcgacaa atttgctgcg atcctcgtct gtttattgaa gattaccaag gcggttccgg
    43201 caagctcgaa caagtaaaag atctactggt agctgccaaa gagaacaaac gaagagtttt
    43261 gctgttttct caatttacag ggatgctgac tatcttgcaa gaagagttag ccgagctagg
    43321 gatctcgaca ttttatttaa gaggcagtac gaaaccacaa gatcggttaa gtatggtaga
    43381 cgcatttaat gctggcgaaa aagatgtttt tctgatttcc ttaaaggctg ggggaactgg
    43441 attgaatctc acgggagcag atacggtcat cttgtatgac ctttggtgga atcctgccat
    43501 tgaagaacaa gctgctggac gtgcacatcg gatcggacaa aaaaatgtgg tagaagtgtg
    43561 gcgtatgatc tctgaaggaa cgattgagga acgcatggat tcactccagc aagaaaaacg
    43621 cgaactgttc caaaaagtga tccaaggtaa tgaagaacag ttgacgaaac tgactgaaga
    43681 agacatccgc atgattttaa gtgtaggaga aatcgaggag taagagactc gaaaaagcgt
    43741 tcagtaatgg gtaagaaagc ctttctaaag aagcgattgt ttatttgatt taaaaaatag
    43801 caaggaggct gggattccat tttatcccag cttccttgct atcatttttt tatttctatt
    43861 tttaaaggtt atacaaagat tgtcttcatt gtctctaaca gctgatcaaa agaagaaatc
    43921 tcgatatcgc aaattggagt ctgttcaatt ttgcgttctc gatggttgaa ccaaagcgct
    43981 tgccagttag cactttttgc acctagaaca tcattgtcgt aattatcgcc aacgtataag
    44041 gtctcttctg gaagcatatg aaattctttc tcagctagct gaaaaatctc tttttctggt
    44101 ttttggaaac ctgttgcttg agaaatgagc atattgcggg aaggaatcca attgttcaat
    44161 tgaagttgct tcagtttttt tgtctgatgg tcagtcggcc catttgtgat aattcctaat
    44221 gggacattct tttccttcaa gaaatcgagg gtttttttga cctcctcatg caagcagata
    44281 ttatcaagtt cttcctcgta aattttttga aataagaggc catcttcttc cgtaatatga
    44341 gggtagtcta aatcttttag tgattggctg atccgatgtg ctcgcatata ttctagtgtc
    44401 caatctcctg ccattacttt tgggaagttt tcatcactat gatggcggaa ccggatatat
    44461 agtggatgca tatctgcatc tgaaacaagc ggaacgactc ttttgaccgc gtttcgaaat
    44521 ggctgctgct gatcatacat cgtatcatca acatcaaaca caactgattt aatcaaaatt
    44581 ttacacgcct ttcggttttc attgattttt tcacaaatcc ttgactattg taacagaaaa
    44641 aattgtctaa aaaagctgat aaataaaggt ttttcttact atatttctat ttccttgaca
    44701 gaataaggtt tttatatttc agtttttttc actaaaaatc gtgaaacacg tcaataagtt
    44761 tgatgaacaa attaatcatt atggtagaag tcgattgatt tattttaatg caatctctgt
    44821 ggaattttcg aaagatttat ttttttaata cagtctcaat ataactattc aaacataaaa
    44881 taacgttatt tgatttattt gtttatagta aattttattt agtaatcatt gctttaaaat
    44941 actacgctta tataacagag aaaataagcg ttataataaa gaacagtatt atttaaaatg
    45001 acagaaaaca ttactattaa agaaataaaa aacataaaaa atcttaagaa atcggtttca
    45061 tttagct
//
LOCUS       DACDIP010000016        39400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDIP010000016 DACDIP010000000
VERSION     DACDIP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.016"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            176..574
                     /locus_tag="IV257_001061"
     CDS             176..574
                     /locus_tag="IV257_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8814207.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            811..1353
                     /locus_tag="IV257_001062"
     CDS             811..1353
                     /locus_tag="IV257_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8814208.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1436..1987)
                     /gene="lepB"
                     /locus_tag="IV257_001063"
     CDS             complement(1436..1987)
                     /gene="lepB"
                     /locus_tag="IV257_001063"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8814209.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(2139..2366)
                     /locus_tag="IV257_001064"
     CDS             complement(2139..2366)
                     /locus_tag="IV257_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8814210.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2363..2881)
                     /gene="msrA"
                     /locus_tag="IV257_001065"
     CDS             complement(2363..2881)
                     /gene="msrA"
                     /locus_tag="IV257_001065"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8814211.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2901..3614)
                     /locus_tag="IV257_001066"
     CDS             complement(2901..3614)
                     /locus_tag="IV257_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8814212.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3617..4513)
                     /locus_tag="IV257_001067"
     CDS             complement(3617..4513)
                     /locus_tag="IV257_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8814213.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4671..5513)
                     /locus_tag="IV257_001068"
     CDS             complement(4671..5513)
                     /locus_tag="IV257_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8814214.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5645..6298
                     /locus_tag="IV257_001069"
     CDS             5645..6298
                     /locus_tag="IV257_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8814215.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6361..6888)
                     /locus_tag="IV257_001070"
     CDS             complement(6361..6888)
                     /locus_tag="IV257_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8814216.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6908..7855)
                     /locus_tag="IV257_001071"
     CDS             complement(6908..7855)
                     /locus_tag="IV257_001071"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8814217.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7867..9753)
                     /locus_tag="IV257_001072"
     CDS             complement(7867..9753)
                     /locus_tag="IV257_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8814218.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(9913..11991)
                     /locus_tag="IV257_001073"
     CDS             complement(9913..11991)
                     /locus_tag="IV257_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8814219.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(12004..12279)
                     /locus_tag="IV257_001074"
     CDS             complement(12004..12279)
                     /locus_tag="IV257_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814220.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12641..13096)
                     /locus_tag="IV257_001075"
     CDS             complement(12641..13096)
                     /locus_tag="IV257_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814221.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(13250..14458)
                     /locus_tag="IV257_001076"
     CDS             complement(13250..14458)
                     /locus_tag="IV257_001076"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8814222.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14570..14902)
                     /locus_tag="IV257_001077"
     CDS             complement(14570..14902)
                     /locus_tag="IV257_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8814223.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            15050..15925
                     /locus_tag="IV257_001078"
     CDS             15050..15925
                     /locus_tag="IV257_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8814224.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(16007..16588)
                     /locus_tag="IV257_001079"
     CDS             complement(16007..16588)
                     /locus_tag="IV257_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8814225.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16594..17853)
                     /locus_tag="IV257_001080"
     CDS             complement(16594..17853)
                     /locus_tag="IV257_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8814226.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17855..18901)
                     /locus_tag="IV257_001081"
     CDS             complement(17855..18901)
                     /locus_tag="IV257_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814227.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFMWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(19136..19411)
                     /locus_tag="IV257_001082"
     CDS             complement(19136..19411)
                     /locus_tag="IV257_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8814228.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19654..20964)
                     /locus_tag="IV257_001083"
     CDS             complement(19654..20964)
                     /locus_tag="IV257_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8814229.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(21146..22375)
                     /gene="rpsA"
                     /locus_tag="IV257_001084"
     CDS             complement(21146..22375)
                     /gene="rpsA"
                     /locus_tag="IV257_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8814230.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22510..23190)
                     /locus_tag="IV257_001085"
     CDS             complement(22510..23190)
                     /locus_tag="IV257_001085"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8814231.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(23239..23874)
                     /locus_tag="IV257_001086"
     CDS             complement(23239..23874)
                     /locus_tag="IV257_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8814232.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23940..25343)
                     /locus_tag="IV257_001087"
     CDS             complement(23940..25343)
                     /locus_tag="IV257_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8814233.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(25330..26361)
                     /locus_tag="IV257_001088"
     CDS             complement(25330..26361)
                     /locus_tag="IV257_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814234.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26392..26613
                     /locus_tag="IV257_001089"
     CDS             26392..26613
                     /locus_tag="IV257_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8814235.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26778..27515)
                     /locus_tag="IV257_001090"
     CDS             complement(26778..27515)
                     /locus_tag="IV257_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8814236.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27668..29008)
                     /locus_tag="IV257_001091"
     CDS             complement(27668..29008)
                     /locus_tag="IV257_001091"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8814237.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(29245..30768)
                     /locus_tag="IV257_001092"
     CDS             complement(29245..30768)
                     /locus_tag="IV257_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8814238.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(31147..31758)
                     /locus_tag="IV257_001093"
     CDS             complement(31147..31758)
                     /locus_tag="IV257_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8814239.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31881..32006)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(32088..32804)
                     /locus_tag="IV257_001094"
     CDS             complement(32088..32804)
                     /locus_tag="IV257_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8814240.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32810..33379)
                     /gene="scpB"
                     /locus_tag="IV257_001095"
     CDS             complement(32810..33379)
                     /gene="scpB"
                     /locus_tag="IV257_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8814241.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33363..34160)
                     /locus_tag="IV257_001096"
     CDS             complement(33363..34160)
                     /locus_tag="IV257_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8814242.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(34130..34501)
                     /locus_tag="IV257_001097"
     CDS             complement(34130..34501)
                     /locus_tag="IV257_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8814243.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34520..35407)
                     /gene="xerD"
                     /locus_tag="IV257_001098"
     CDS             complement(34520..35407)
                     /gene="xerD"
                     /locus_tag="IV257_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8814244.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35503..35976)
                     /locus_tag="IV257_001099"
     CDS             complement(35503..35976)
                     /locus_tag="IV257_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8814245.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(36081..36944)
                     /locus_tag="IV257_001100"
     CDS             complement(36081..36944)
                     /locus_tag="IV257_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8814246.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(37140..39239)
                     /locus_tag="IV257_001101"
     CDS             complement(37140..39239)
                     /locus_tag="IV257_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8814247.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        10882 a         8198 c         6678 g        13642 t
ORIGIN      
        1 taaagaaatt ccatctatta gttaagtgaa agtgaaccag tcctcattct atcacatctt
       61 cacttttttg atgatgcaaa tttttatttt ggtttctata tatgaatcta aatttttgac
      121 agcgtttcct aaataagaga tgatagacga acaaaacaga aaggagacga aacaaatgaa
      181 tagtatggtt tttgttaatt ttcctgttgc tgatgtcaat cgttccgttg ctttttatga
      241 aaagctgggt tttaaaaaaa atgaagaatt ttctactgaa gaagctggtt caatggtatg
      301 ggatgacact ttttggataa tgcttctctc tcatgatttt tataaaaaat ttataaaaga
      361 taaagaaata gctgataata aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa
      421 tgcggatgaa gtaaaaaaat ttgctcaaat tgcaaaagaa aatggtggta ctttccacga
      481 agtagatatg ggaatgcctg aagaagaaat gttttcatta gaagtccaag atctagacgg
      541 aaatacatta gaacccgtat ggatgaagat gtaagcagat aactaatctg acgtttttta
      601 taacaaaaaa acaaaggcgg atgatacgat aagtattctc cgcctctatt ttttttattc
      661 gtacaatctc ttttttacag tttataaaag cagtcttctc accctttttt taattgttag
      721 atgccagtgt atgtaaatca ccaatgtgtc atgacacatt ggtgatttta tggtatcctt
      781 ttattattaa ctgacaaagg agagaatatt ttgaacactt tgattgcaaa agacgttttt
      841 agattgcccg aaatgaatgc aagattgttg tcgacctctg ctcttttatt agcccttgat
      901 gtcatcttat acaagatagc gattggtcct gcttattttc aaatcacatt cggattttta
      961 agtatggcgc ttatgggtta tctatatgga ccgatctggg ctggtttgct tgaaatgttg
     1021 tcgaacactt taaatttcac gatatttggt tcaggaacgt ttcaagctgc ttttcttatc
     1081 acggctttcc ttggtggatt tattaatggc ttatttttat atcataaaaa gataaacctg
     1141 ccttccgtca tcgctgccca attattgatt atgatcccag tcagcctttt tatcaattct
     1201 tggctgatgt ctgttttgtt tggtgcagat ttcaaagtaa tttttctagg tagattcatc
     1261 aggaatatga tccaaatccc tattcaaata atggttgttt attcatttat caaaacttta
     1321 gaggctagaa aagtgtttaa acgagctgtt tgattttacg tactaaggta gtaaactgat
     1381 tttggattaa acaataaagc gtgcagtctc aacagactgc acgctttatt gtttctcact
     1441 ggatccattt tatttcatca agtggataat agacaaatct tgctttacct agaatatcag
     1501 ttttattgat cgtgccaaac gaacgactat cttttgaaac tcttcgatta tctccaagta
     1561 caaaataatt atcttccgga agcttggatt gtcccgttag ctcttgtagt gtaaagtccg
     1621 tcgtatagga tgcatttgca tgatctgatt tcaggttttt agttagataa ggttccttaa
     1681 ctactttccc attgatcttc aattgatcat tttggtagct gacattttct cctggaagac
     1741 cgattactcg tttgatataa atcgtgccat ctgctaattg aaaaacaaca atatcaaatc
     1801 gtctgatctc cgaaaatttt tccatcacaa ccatatcacc ttgcttcaac acgttttcca
     1861 ttgaattgcc ttccactggt accgggatga ataaaaatcc ccgcaacatg aacgcaagga
     1921 atacagaaac aagtaagtat ttgaatacaa gccagaagcg attaatatag cgttgttttt
     1981 ttgtcaatct ctatcaatcc atccctcttt ttttcattct ttctttatta taagaaaaat
     2041 atcaaaaaaa tgcgactaga tagctgaaat aaaaataatt ctttcaatta ggctattgga
     2101 caaaaaactt ttttgatcat aaagtacaaa actatctctt atatgtcatg attattttct
     2161 aaatattttt cccatgcttc gtcaaagata tccatggacg gcaaatagtc gacattcagt
     2221 tccagataat tagaaatttc ttcgtagcta gagctgtgct taggaaattg gatgtcttta
     2281 ccggcttctg tagcgaaaac agataaagca tcatgtgtgc caccgcctcg cagtgtcatt
     2341 aaataatgaa aaaagcttcg attcaccctt cgttcctcca attttcttcg ataaaggcag
     2401 cccggcgtgc atggtttctt ttaaaatttt caggatgctt tttataatac tcttgatggt
     2461 cttcttctgc tagataaaat ggttgggctg gttcgatttt tgtaacgatt ggctgttcga
     2521 aacgtccact ttcagctaac tgtttcttac ttttttcagc aatgatccgc tgttcttctg
     2581 tcaaataaaa gatcaccggg cgataattgt caccgcgatc ctcgaattgt ccgaaagcgt
     2641 cagttggatc agtttgctgc cagtaaagtt ctactaattg ttcatatgaa atcaatgttg
     2701 gatcatagat aatctccact gcttctgtat gtccagtcgt ttggcttttt acttgttcat
     2761 aagtcggatc tggtacatgc cctccagtat atcctgacat cactgtatgg ataccaggaa
     2821 gcgtgtcgaa aggctggatc atacaccaga agcagcctcc tgcaaatatt gctcgttcca
     2881 ttatgattcc ccctctaata ctactcatcc gatgattttt tattttcttt tgtcttgctt
     2941 tcagtactgc tggtgctagt tttgttggta tttgagttgt ttgaagcatc agaggtatct
     3001 tttggcaaat atagactaaa ccgaatgtca tcatttacta agtcgatttt atctgcttta
     3061 atatacatcc cgttttgcat tcggaattga tccaacctaa gcatgatagt cagatcatct
     3121 gtattgactt cgatccaatc tggtaattta tagtttctct tgatcatatt catcacatct
     3181 ttgattggca aactcaatgt tccgatagaa agacttctag cttttagttg gacatttccg
     3241 ttttccatca catacggatc aaaatataaa tagaaatcga taggaaaacc tagtacttcg
     3301 aaagtaccat tgagtagtgc ttcattttcc aaataaaatt tatattcaat atcactatct
     3361 ttttgatact cactaaggaa aaagctgatg atttgattga ctttttcttt atttgaattg
     3421 atcgttaaga caggatctcc ttgtctttcc gtaagagctg gtatttctgg gagttctggt
     3481 tcccgattag caaaaatacg gctgcctaaa aatacggtcg aaccaagtac taccccaaga
     3541 aggataagga aggcgaattt ccagtaattt gttttctttg ttttgtttga agatacgtca
     3601 gatcttgttt tcatgtttat tgatcatcct ccttctctaa ccattggttt tgtgtctcga
     3661 tcagctgatc tctcactgca tttgccatca attgataacc taaattattt ggatggaaat
     3721 gatcttcttc atagagcaca ttatttttga tgccatttag attttctgtc gtgctagttg
     3781 tttgatctgt tccttcttct gtgacaccta cttcctgatt caatccttga taaagcaaat
     3841 cattgattgg gataaagtag cagttcggca ttttttttgt caccgtttct gttcctttgt
     3901 tccaattgtt aacaattgtc tgcatgtcag tgatctcagg aaagttcaaa taaaatggat
     3961 tgtagatgcc taaaatgtaa ataggtgcgg ttttattgag ggatcgaaat tcttccaata
     4021 gttctgtgac attttcctga tagtttttca gtggcttttt aaaggaattg accgttaacc
     4081 cgaataaatt attttggata actttcatca aatcattacc gccaacagtc agtgtgataa
     4141 aatcagctgt tttgatattg tttcgaattt cttcttttgt ttttactcgc tttaagatct
     4201 ggtcacttcg ttcaccattt acaccatagt tttctgtttc aaccgcggtc aaattataac
     4261 ggtcttttag atcatttgcg acaaggggaa cgaatcctcc ttgcttggtt tgatctccta
     4321 ctccttcagt cagcgaatct ccgatagcta tgtagtgaag tttttctttt ttatttggat
     4381 cttctctcac agtagttact ggtttcatta gtgggccagc tttcggtata gaagcagcta
     4441 aacctgcata gatgatgatc gcaattaata atgggaacaa aacgaggatc aaatgtcgtt
     4501 tcgttaattt cattttttca cctcttgtat tcgattggtt ctaatgttat caaaaatttt
     4561 atcaaaaagg tagtctttat gaaaaaagaa aacaattatt ttcctaattt tataaagaaa
     4621 aagagaataa gctgcaagcc tttcaggctg agggcttatc ctcttttcga ctaatctaca
     4681 taatacatga ttgcaaacgc gtctttacca gtatgcgtag cgataaccgg attcgtatgc
     4741 agtacaggga tatgcatgtc cgggaataat tcttgcaaac cctctttgaa ttcttgcgcc
     4801 aattcttttg catccgcatg tgaaataccg atttgtcgca catttggcaa ttctttcagt
     4861 tcatttttca aattattaaa ccatttatcg aacgttttct ttcctcgccc cttagttaca
     4921 gggattaatt cagaatgggc gaaattcatc accacgcgta tgttcaaaac actagacagc
     4981 aaaccagttg ttcggctgat tcttccacct tttaccaagt tatctagtgt ggatattccg
     5041 atgtataatt ttgtattgtc ttgtactttt ttgacttctt caaggatttt ctctactgtt
     5101 ttaccttctt ttgctaattc agctgctcga attacctgaa aagataatcc ttggtcagtg
     5161 gtatcactgt cgataaccgt tactttcgtt tttgttagat tactggcttg tcttgctgct
     5221 tctactgttc cactcaagcc ttttgtcata tggatcgaaa tcacttcact gccgtcttct
     5281 cctagctcat catacatcga gatgaattct ccaattggtg gttggcttgt tttgggcaga
     5341 ttttttgctt tcgccatcat ttccataaat ctttcgccgt ctagttggtc atcatcggga
     5401 tatactgttc cgtcgatcat gatggatagc ggcatgacac gaatgcctaa agcatcgcgt
     5461 acattttttt ccatcgtaca tgaagaatct gttacgattt ttacatttgt cattattttc
     5521 tctctctctt tctttaacaa gatgccttat tcatgttaaa ctacatgtaa gacaatttct
     5581 atttttagta taacagacaa ataatgattt ttcattaatt acacctagta aaggagccgt
     5641 tttcatggaa aaaacgaaat ttaatcgaac gtatgaaata ttaaatgaag tgtttaatgc
     5701 agtcacccat ggaattggga ctggattgag tatcgcggga ctagtggttc ttttagtaaa
     5761 aggtgcccac ttgcattcct ccctccatat tgtctcttat gctatctatg gatcgatgat
     5821 gattctttta tttttaacgt ctactttgtt tcattcattg atttttacaa aagcaaaaaa
     5881 ggtgttccaa gtattcgatc atagttcgat ttttcttttg attgcaggaa gttatactcc
     5941 tttttgtttg ctgagtatcc gaggatggct aggttggact ttatttgtat taatctggtt
     6001 gtttgcgatt cttggcatta tatataaagc gttgacgttg cataaacggg agagagtatc
     6061 aaaagtatct acgatcatct atatttttat ggggtggctc tgtgtcgtgg ctgctgttcc
     6121 tctttatcac tctcttggaa tgactggtgt tattttgatg gtagcaggcg ggctttctta
     6181 cacgataggc gcattctttt atagcttgaa ttctatccgc tatatgcatg ttgtttggca
     6241 tttgtttgtt atgttaggtg caggttttat gtacttctct gttttattct ttacttaata
     6301 aaaaaaataa agctatggat gggacagctc taaataacag ctagtctatc catagctttt
     6361 ttatatcgtc aattcgcctg tcccgctttt tacaggattc tcattacgct gatacgtttc
     6421 aaattgatgc ggataacgat ttttttcatc catttctcct tgactagtat tgatcagtga
     6481 ccaatcagac cagtcaacag gtggaaaata agcatctcca tcgaatacat gatggatgac
     6541 tgtacggtat aatatcgtac aaaatggcag aaattcttga taaacagctg accctccagc
     6601 gatgaaaaag ataccgtctg tttctttttc cagagcaagc acgtcttcca cactatgcat
     6661 gacatgcacg ccttctgctt tataggaaga atctctcgtc aaaacaatcg tctgacgatt
     6721 cggcaacgga cggctaccca tcccttcgaa tgtttttcgt cccatcacta gtgtatttgc
     6781 ttctgtcatt tgtttgaaga atttcagatc atttggcaaa cgccaaggaa gacggtcttc
     6841 ttttccaatc aatccgtttt catcctgtgc ccaaattgca gctaacattt tctcatcctt
     6901 tctgattcta aacagcgata ggagctttga tcgccggatg tgggtcatat ccttctaatt
     6961 ggatatcttc catctcaaaa tcaaatactg actgcttttc ctgattcaaa actaaagtcg
     7021 gaaatgcgcg tatttctcgg ctcaattgtt ctttcatttg ttcgatatga ttgatataaa
     7081 gatgagcatc tcctaatgtg tgaacaaatt cgccaaccgc taaacctgtt tcatgtgcaa
     7141 ttaggtgagt cagcaacgca taacttgcaa tgttgaacgg aacgcctaga aacacatccc
     7201 cactgcgttg atacaattgg cagcttagcc gtccctcttg aacataaaat tgaaacatcg
     7261 tatgacaagg aggcaaagcc atgctaggaa catcttccgg attccatgca gaaacgatca
     7321 atcttctaga atcaggagtc tttttgattg cttcgatcac ttgtttgatt tgatcgataa
     7381 agccaccgtc ttttgtttcc cagtgacgcc attgatagcc gtagatgtgt ccaagatcgc
     7441 cgtatgtttc agcaaaggaa tcatcggtta ggatcttctc gcaaaaaagc ttgctctgct
     7501 ttttatattc ttcattgaat tcaggatctg tcaaacatct atggccaaaa tcagtcatat
     7561 ctggtccatt atagtctgga cttttgatgt agcgttcaaa tgcccattca tcccaaatat
     7621 ggtttttatt ttgtaataga tagcggatat tcgtatctcc ttttaaaaac caaagcaatt
     7681 cgcttttgat caatccaaaa ggtactcgtt tcgttgttaa caaaggaaac cctttttgca
     7741 gatcaaaccg catttggtat ccgaataagc ttaatgtgcc tgtgcctgtt ctgtcttctt
     7801 tacgatgccc ttcttccaac aattttcttc ctaagtcaag atacgcttgt tccatccttt
     7861 ttcctcctaa tcttctaccc attcactcaa gtattcccac cgtgccattt tttcttcaag
     7921 ttgtgcttct gtttcagata cgtcgttttg caattcttgt aatcgagtga aattatctcc
     7981 ttgatgattc atctcttctt ggagcaatga gattttttct tcaagttctg caatctcatc
     8041 ttcaatcgtt tcccattctt tttgttccat gtaagataat ttcttctttt cagcaggttc
     8101 cttgctctgt atttttggtt tcgctttttc agcaggctgc tgggtctctt tttgcttcag
     8161 taaataactg gacatgtttc cgtaataaag ttcttgtttt ccgtttcctt ggaatactaa
     8221 taacttatct gctaccttgt ccaagaaata acggtcatgg gaaacagtga tcacagcacc
     8281 tggaaaactt ctaaaataat cttccaaaat cgtcaatgtt gctatgtcca aatcattggt
     8341 cggttcatcc aataataaaa cattaggttg ctgaatcagc agtttcaaca aaaataaccg
     8401 gcgtttttct ccgcctgaaa gtttgcctat cagtgtccca tgcataaatc ttggaaataa
     8461 aaatcgttca agtaattctg ctactccgat ttgactgcca tctgctgttt ttgcttcttc
     8521 agcagcttct tgtaagtaag caatcatccg tttatctggt gccatttctt cattttcttg
     8581 tgtataatac gcaagatgaa ccgtttcacc gatagacatt gtaccgctat ccaggggaat
     8641 tctgccagct agaatattaa gcaatgtcga ttttcccgca ccatttttac cagtaatacc
     8701 tagtctttcc cgagattgaa tcaataaatc taaatgttct agcagtgttt ggtcatttat
     8761 acggtaactg ccatctttga tttccagtac ttttttccct aaacgttgtg tcgctaggtt
     8821 cagctctaaa tcatcttctt ggtttacttg gtacaagttc tcctttagat cttcgaaacg
     8881 gttgatccgt gcttgctgct tggtgccacg tgcctgtacg ccagccctca tccaagctaa
     8941 ttcctgttta taaagccgct tgcgcttttc ttcttgttct acttctacac gatcacgttc
     9001 tgctttttct aagacgtaag cttcataatt tcctttatac tcgtataatt ttccaaatga
     9061 aagttcgaag attcggtttg ctactctatc tagaaaataa cgatcgtggg taaccatcaa
     9121 tagtgcgcca cggtattgtt tcaaatagtt ttccaaccac tcgatcgctt cataatctaa
     9181 atggtttgtt ggttcgtcta gtaatagtaa atcgggttca tcaatcaaca cttgcgccag
     9241 actaactcgt tttttctgac cacctgataa agtactgatt tttttatcta attcactgat
     9301 tcctaatttt tgcaaaataa tctttgcgtt ggtatctgta gtccaagcat cttctttatt
     9361 catttgttct tctgctttag cataacgttt ttgcacatca gggtcttctc cgttttcccc
     9421 tagttcgatc aatgcttcct catatgctcg aacagtttgg atcaaggggc tgtttccttg
     9481 aaagactgct tggagaacag ttaattcatc ggaaaattca gctgcttgag aaagataacc
     9541 aatacggtaa tcagttggat aaaacactgt ttgccgatca ccgtcgctgc tatcgatacc
     9601 ggcaataata tttaagagac ttgttttacc cgtcccgttc gtcccaatca acccgatacg
     9661 gtctttttcg tggaccaaaa aagaaatctg gtcaaacaaa tctttttcac cataagtttt
     9721 tttgaggtca gttactttta attctttcaa catgttccct ccttatcttg gatattgtat
     9781 cataaaaata gtgaacttca ggaaaatttt tttggtattt gttctttttg tgttctcaaa
     9841 aaaagaacag gaccgattcg atcctgttct gatatgtttt gttttttata aaacttgaaa
     9901 atttttacct ttttatttta ttggactcgt tttttttgac tttgctcgaa acgcaaatcg
     9961 gaaagaaaag ttatcagatg gtttgttcat cgtataagat tctaacaaat tgatattcct
    10021 gcgatagctg ttcgcagaaa acgtcgtgat tttttcagca acttcgtatt catcaatgat
    10081 ttttctttca agataataac ctcttagcat ttttttcgta aacagactat cttgctggat
    10141 catccacgaa gaaatcaaat tccccgagtt catttttatt ccttcaagtt tgcgttgctg
    10201 gataaaaaat tgaaccatcg tttcatcata aatatcagtc aagttatcta aactttcaat
    10261 aatcaactga gtattttttt gatatgtctc tctgatttct tggatatctt catcactaat
    10321 aaatgtctga cgatcagcat atcttctttg ccaaaacatt ttcggatgga gaacgattcg
    10381 cattaaccta cggacaacaa tgaaccaaaa gccgataaaa gacaaaatct ggcttgtgat
    10441 agaatgttcg aatcgagaaa gaaaacggga gtaaaaacga taaccattag aagaaagctt
    10501 gcccgaacga taactttcat ccaatccatc ccgttcgatc gacaaaatca gtgcttgtat
    10561 ttcttgtact tcttgctttt catcatcggt taaatcttcc aagtacagat cacgtatccg
    10621 ttcttgataa ttttctatca ctgcttcagt ggctaaaaat tcttttgtat tacgatcaat
    10681 ttcctttatt tcttttcgca aaacttcaac gacctcttct aaaatcatca aagcattcaa
    10741 atccgtgctt ttcgcttcct ctgtttccgt cagcataggc aagacaatga tccctatgac
    10801 caaagtcact aagatgacac ccgccgtcag aaacaaaagc aaagaccgct ggtaaaacat
    10861 catattattg attgaaaatg gcaaaataaa aatcgtagcc aaactgactg ttccctttac
    10921 accgccaaat gttaagatca agatctcgtt caactgtttt ttgaactttt tactgccatc
    10981 tttgaataca taaaaaagac tgatagaaat gaatctgatc acaaaaagca tgacagagat
    11041 taatacgata atcgctaata aaagaccgtt tggatataaa ccgtctcccc atactggcga
    11101 gaacacttgc gtcaattcga ttcctaaaaa tataaatact aatgcattca gtgtaaaagc
    11161 aatagttgtc catgtactgt gggacaagct cgacaattct gcatcaaaca cagagatttt
    11221 cctaaaacca gaagcttgga gaatacctgc tgccactgca gcaatgattc cagatacatc
    11281 aaatgcctct gccaatacat aggctagaaa aggaagcaaa agttcgatca gcagataccc
    11341 tgtgacatct tgtgctgatg ctttttcgat caaatgaatt attttttgtt tgaaccagac
    11401 aagcaaaaag ccgatcaaag ctcccccaat actagaaact actagactca tccccgcgtt
    11461 tactgctgaa aaagatccgg tgatcagcgc accaagagca aactggaagg cagtaacacc
    11521 agatgcatca ttcaaaagac cttctccttc caaaatatgc atcgcctttt tagggatatt
    11581 cactcgtcca gaaagagaac tgactgctac cgcatctgtc ggacctaacg ctgctccaaa
    11641 agcaaagcag gcagcaagtg ggacacttgg caacaggaat gataacgtcg cacctacccc
    11701 gacaagggtc aaaatgactc ctccaaatgc caagaacaaa attgtcccga aatttttgag
    11761 aatcgaagaa atatctgctt tttctccttc tctaaataac aatggtgcaa taatcataac
    11821 taagaacaat tcaggttcaa aatcgattga ttgtccccac tctgtcagac ctaataccac
    11881 tccaaggaaa atttggatca gtggagctgg aatcattggc aaaatgcggc tagctatgtt
    11941 cgataaggtg atcgttactg caaatacaat aactaactca gcaaattcca tcgtccatcc
    12001 cctttatttt ttattttgtg taagcatttt taaaaattgt tcttttttct ttaaaatttt
    12061 ggtttcgact acttcaacct gtgtttggtt cccctctaat tcacactgta cacgttttga
    12121 ctccaaacga agcagatcat gtttcagacg ttccaactga cgatacatca atatatttct
    12181 tttacgcaac ttacaaagta tttgacggcg gtcatccatc gaaagggcgc catagcgatc
    12241 atgcaacagt tttttttgtt gttcaaaact ctctctcatt ttccagatct cctatacaca
    12301 aacttccatc attatagcaa agttcttttt ggatgaaaaa tatttcattg tttcatcgtt
    12361 tattgctagt tagaaatttc cataaatttt tcaacgcctc attctattcc aaatagtatc
    12421 agatatcaag taaaacttaa gaaagataaa agtttctgac aaaaggatgt ttactttccc
    12481 attcctgcct ttcttgttct tttatcacaa caaaaaaatg gggctgcgac aaaaatcttg
    12541 tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt agtaataaga
    12601 cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa aattccccgt
    12661 atacatcttc tacacgttga tctggattga tcacgatata caagttacct gttttttcgc
    12721 tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct agatatggga
    12781 agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg atatcttcgt
    12841 atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata tcatgtgtcg
    12901 caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga tggatttcaa
    12961 caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc caaagatcgc
    13021 cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat tttagcgctg
    13081 tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg tactacctca
    13141 gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa tgacgtatct
    13201 gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt taaatcaatt
    13261 tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt tgttctacga
    13321 cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt tttccagttt
    13381 cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata ggtaaacgat
    13441 cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc atcaattctt
    13501 ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc aaagctccag
    13561 tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt tggttagaac
    13621 acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt tggctgatca
    13681 ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca gcaaaacgta
    13741 aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct gtttcaataa
    13801 atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat tccacattta
    13861 tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg tagaaggttt
    13921 cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc attcgtaagg
    13981 catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt tcttctagat
    14041 cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca aacgcattga
    14101 tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg acatgatctg
    14161 gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca tattgttcat
    14221 cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt tttggaaaaa
    14281 gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg attggctggt
    14341 tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct gcattctcta
    14401 attttttcag aactggaatt gccttttggt attccagagg taattgtttt aatttcattt
    14461 ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca tggtgactgt
    14521 ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt tactcgtcct
    14581 tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt tcaaaaactt
    14641 cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa acatcaccaa
    14701 gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct ccataagtgt
    14761 ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc tgagctaatg
    14821 gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc tgcatactgt
    14881 ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa aactttaacg
    14941 caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt aggaaggaat
    15001 ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata tggtcaaacg
    15061 aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg catttggtct
    15121 tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga tcaccttgat
    15181 cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca acatcccatt
    15241 gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg ttctaggtac
    15301 actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa tcaatttaca
    15361 gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag gaagcgggat
    15421 cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga ttttagaaca
    15481 aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg tcttacttgc
    15541 ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt cgtatgtttt
    15601 cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc tacttgtcgt
    15661 ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa gaggtgtgac
    15721 tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct atatggtcgt
    15781 tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg aaaaagcttt
    15841 cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt tgaagccaca
    15901 gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga ggaaagattc
    15961 cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa ttgttatgtt
    16021 cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta acttcatcag
    16081 ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt tgagcataaa
    16141 gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt aattcagtat
    16201 cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag atagatagat
    16261 attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc tttttccaat
    16321 tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc tgcaaaaaca
    16381 atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga tccgcaaaat
    16441 tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca agataattca
    16501 agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac gttaaaaaat
    16561 tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt catctaataa
    16621 tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata attcttgcgc
    16681 tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga aatctggttc
    16741 atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa agtcttctaa
    16801 ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt cttccatctg
    16861 attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat tacttaaagt
    16921 aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa gttcttctgc
    16981 tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa gttcgacttg
    17041 gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa tcatttcttc
    17101 ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat taacttctcg
    17161 aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat tcgtaaaagc
    17221 tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta aaggtacgat
    17281 agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct gcatcgagat
    17341 tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg ctgcctcttt
    17401 gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg gttcatcagg
    17461 aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc cgataacttg
    17521 gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct tatccacttt
    17581 ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt ctgctaacgg
    17641 gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa tctgttttgc
    17701 ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata tatctgctgg
    17761 gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt catctgataa
    17821 agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc tcactgacga
    17881 tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt tggtatccgt
    17941 ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc aaaaaaagaa
    18001 ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga tagccaagca
    18061 agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct tggagattcc
    18121 acatgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt agatggccat
    18181 tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg ttttctgcct
    18241 ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga gtcgttaatt
    18301 gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca caaatcattc
    18361 cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc tcgctgattg
    18421 tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca cgatttggta
    18481 gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc gatacaggga
    18541 tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc agatccatga
    18601 tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag cgtttgacga
    18661 aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca caaatatacc
    18721 agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac aaatcataat
    18781 agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt agctgactga
    18841 ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa tcaagttcca
    18901 tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc tagaaaagat
    18961 aagagcaaag ctcacctcat aacttgcaaa taaataaaaa ttaaataaaa tacatgtgtt
    19021 ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt ttctgctagg
    19081 aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt
    19141 taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt
    19201 cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa
    19261 agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg
    19321 aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa
    19381 ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata
    19441 tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat
    19501 tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt
    19561 taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc
    19621 ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg
    19681 gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata
    19741 agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc
    19801 gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat
    19861 tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt
    19921 catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga
    19981 aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa
    20041 atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga cgataataat
    20101 tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc
    20161 ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat
    20221 cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc
    20281 tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt
    20341 cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga
    20401 ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc
    20461 ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc
    20521 atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc
    20581 gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt tattacttct
    20641 gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac
    20701 acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat
    20761 aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa
    20821 ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga
    20881 gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac
    20941 gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag
    21001 tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat
    21061 tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc
    21121 atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct ttttcttcta
    21181 cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta ttttcttctg
    21241 gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt tcttctaaag
    21301 ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact ttcactttca
    21361 cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag
    21421 agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg aagcttgtca
    21481 aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg atttctgacc
    21541 aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca ggattgatag
    21601 acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt tttgctacat
    21661 gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg tcgacaaatg
    21721 cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca tggatagttg
    21781 aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta tgcgataaga
    21841 tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt ttgcctttat
    21901 agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca aaaccacgta
    21961 caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct tcgatgacct
    22021 tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct aaacggcgtt
    22081 ttgaaagtag atagctgccg ttttctttat ctttgccgat cgtactgata acgactaaat
    22141 ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa gttgataatt
    22201 cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc acttgtttgt
    22261 cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct tgtacgcttt
    22321 tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct gtcataacac
    22381 ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt aattgattat
    22441 tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt tttcattttt
    22501 tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta ttgctgcaac
    22561 gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg cttgcttcag
    22621 cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt tcgtcagtgt
    22681 ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt ctgcacgctc
    22741 acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag ctgtcccgat
    22801 gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga cagcaactaa
    22861 ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga catctggttg
    22921 acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat cttcttgttg
    22981 cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat ctgaaaaaga
    23041 tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg tatctgtata
    23101 aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag atgaagcagg
    23161 cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca gaaaaaaagc
    23221 cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa gcataaagtt
    23281 gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg cacgctcagc
    23341 cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg cagctgatga
    23401 agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag ttgcctcact
    23461 tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac ctgtatcttg
    23521 agacacagta gattcttccg cgattgtact agattctact gttgaagtct cagttgactc
    23581 ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag aagaagttgc
    23641 tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg ctggaatagc
    23701 gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg
    23761 ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg gttgttccca
    23821 tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc tcactgtttt
    23881 acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt cctaaaaaat
    23941 caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg aagttgttca
    24001 gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc aatatcacaa
    24061 caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg
    24121 cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc
    24181 tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat cagctccatc
    24241 catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa ataaagctca
    24301 aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg ttcatcccca
    24361 ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg accgatttct
    24421 tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg gatatcagat
    24481 ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg attttttaca
    24541 aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata gtatccaact
    24601 gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt tgcacaataa
    24661 atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc tttttgctgc
    24721 gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat ctcttttggt
    24781 ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc tgtcgccgtt
    24841 agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg ttgatattct
    24901 ggacggaaat caactcccca ttgagacacg caatgagcct catctactac atataacgca
    24961 atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc gggactcaaa
    25021 aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt
    25081 aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt cacttgatct
    25141 tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa ataccccgtc
    25201 aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt atcttctcca
    25261 tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat
    25321 ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat agatttgata
    25381 caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa agcgccactc
    25441 tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga gaatccactc
    25501 gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta ctgttcctct
    25561 ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa acatctgctt
    25621 ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca aagcgtgcca
    25681 aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc tgctttgttg
    25741 cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca gccctgttcg
    25801 ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta tctccgaaaa
    25861 cagctgcacc aaactctcat acgcagtatc aattaaaatt tcacgtggca attggctttc
    25921 actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg gaagatagtc
    25981 ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga gccgccaact
    26041 agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta tttgatgttc
    26101 ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta tttcgatcca
    26161 accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca attctggaaa
    26221 actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg aagtcgttcg
    26281 ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg atgaaaatag
    26341 cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga aatgtcacta
    26401 ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca
    26461 gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc agaggctcat
    26521 caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca
    26581 gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta aaaacgtaca
    26641 ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg tgaaatcccg
    26701 acttattttg aaagattcat ttgctatcta tagttaattg actagttttt tcacggaccc
    26761 tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt attgacgatc
    26821 acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg ctcaccatag
    26881 ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct ttccggtaaa
    26941 tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt cgccgtgatc
    27001 gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata acttgccatg
    27061 aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg acttgccatt
    27121 acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc ttctttgacc
    27181 ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac aatagcgact
    27241 gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg ctctacgaga
    27301 gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc tccttcaaaa
    27361 aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc tgacgatggt
    27421 tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc taaaatgatg
    27481 actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt ctatctgagt
    27541 gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag aaatgcaaac
    27601 tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc tttttgtatg
    27661 tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc attgctttat
    27721 acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga gtatcaggga
    27781 acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg ccgcctgata
    27841 caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga tttttcacta
    27901 actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca taagtcaacc
    27961 catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg aatgtaacag
    28021 ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa cttgcaaagt
    28081 tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct tcaccagttg
    28141 cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat acttctgaaa
    28201 gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc gcgtggatac
    28261 ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg tgttttttca
    28321 atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca ggaacaacat
    28381 tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc aagtaagctt
    28441 gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca aatccttgcg
    28501 taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt tcttcatctg
    28561 taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc aaagctttga
    28621 cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga ccaactaacc
    28681 agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg tcccctgctg
    28741 gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca atctctgaat
    28801 aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca ctgatttcca
    28861 atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt cctgaatctg
    28921 cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat gcttgttcat
    28981 gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt aaaaagggtc
    29041 atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta agcctgctcc
    29101 atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact gaatggagct
    29161 attgacttat ccacgtgctt atcgatagcc gtttatcggt ttcttcattc ttatgataga
    29221 ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga tcacacagat
    29281 ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg aaacatcgaa
    29341 acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta agtttactaa
    29401 accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg caaattcacc
    29461 cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag gaatcaagaa
    29521 tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag ataatgaact
    29581 taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt tcataaccac
    29641 ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag aagcaccagc
    29701 taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta ctacgatgga
    29761 cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg gaagcatgct
    29821 tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca atgattcaaa
    29881 aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca tgataccaaa
    29941 agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt cttgacgacc
    30001 gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata ctaatgattt
    30061 tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag aaatagctaa
    30121 caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa tcccaacagc
    30181 ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc ctacttgaga
    30241 ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca tcatgactgg
    30301 gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag agccgcctaa
    30361 tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga atttcttcac
    30421 aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta aaaatccacc
    30481 aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag ttagctcccc
    30541 cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca gcccttgagg
    30601 attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt ctacactata
    30661 gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa taaataagac
    30721 cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct tcttcttcct
    30781 ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa aagagtatca
    30841 gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg tttttcccta
    30901 ttccctcctc ttttttcagg tttttaaagc tttcttcttt ttcattgtag cgtttccttt
    30961 tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt tgcaaagaat
    31021 tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa aacgtttaca
    31081 ttattgcatg tcttgtattt ttttccaaaa aaaaaagacc aagaaatttc ttgatctttt
    31141 tgatatttat ttgactgctg tatgacgttg ttcgaactga tgttgttttc gacttaacca
    31201 tggaagaagt tgcgcatgca gcactaaaaa gacagcactc acgatgaatc ctttgatcaa
    31261 gttgaaagga acgatcccaa tcagtacata gtttgctaaa gacatgccca gcatctgtgt
    31321 tgcgttcagc cctaaaaaca ttaagtaaag aggcgtaata acgaaataat ttgcgatact
    31381 cataaaaata gttagagcga tggttcctgt aatgacacct aaaaatttgt ttcttccttt
    31441 atgctgtttc tttacactaa agaaataata aatcgggaat gtaaagatcg ttgttgataa
    31501 gaaactagca gtgtctccta ccaaattatc cggagaaaaa cctgtagtca gcagatgtaa
    31561 tactgaacgc aagaacgctg ttgcaactcc ggccgctgga ccgaataaga acatgctgat
    31621 caataccgag atatcactaa aatcgatctt caaaaaacta aaagccggca tgatcggaaa
    31681 agcaaaatac tgtaaaacta atgcgatcgc agcaaacatc gctactgcaa ccattttctg
    31741 tacacgggtg ttcttcattt gaaatcctcc tgttaggact cctcttcagc gaactttttg
    31801 acgtttctct ccaaatgaaa aaactctatt ttccagggaa aatagagtta gagttatttg
    31861 atcaagcgtc gtcaaataag ctctatcttc tttatccaga ctatactgtc ggtatcggaa
    31921 tttcaccgat tcagctattt ttttgcaaaa taggtcgtgg actataacca ccggtcggga
    31981 atttcaccct gcccctgaag acgaacctta tataattttt gtttacatat tcattataca
    32041 tatctattca aaaaacgcaa catctaacac ttataaaaag taagtgttca ttttgagtgg
    32101 ttcagcaaac tgctgacttc ttttttgttc agttcacggt aatccccagg acgtaggcct
    32161 tgtaaagtca gatctccgta tttttcacgt tttagttttt gcacaggaag accaactgct
    32221 tgaagcatat ttttcacttg atgattacgt ccttcgtgga tcgtcaattc cacgatactg
    32281 tggtttgtct taggatccac tgataaaatt tggaatttag ctggagatgt ttttcgtcct
    32341 tcgatcttta ttccttttgt caaaggtgca agcatcgatt ttgtagcgat cccttttacc
    32401 tttgcaacgt aaactttatc cacttcatgt tttggatgtg tcaggcgctg agcaaaatct
    32461 ccatcatttg tcagtagcaa gatccccgaa gtatcgtaat ccagtcgacc tacaggatag
    32521 atacgttctt ttaccatcgg caaaagatcc gtaacaactt ttcttccttt gtcatctgca
    32581 acagcagaaa taactccttt aggtttgtac aataaatagt aaccgtattc ttcttgataa
    32641 attgggactt catccacttc cacagtgtca tgtttcccaa cttttatccc taattccctt
    32701 acaacttgtc cgtttacttt tacacgtcct gcaaggatga attcttctgc ttttcgacga
    32761 gaagtgatgc cagcgtgggc aattactttc tgcaatctct ccattacaat tattctcctt
    32821 tattcattcc atcaaagaat agatctggtg acacgtcttc ttcaagtgaa atttccattt
    32881 cttgtgtatc aggcagttct tctaatgatt tcaaaccaaa ataatctaag aagtagccag
    32941 ttgttccata taatatcggc cgacctggtc cttctactct acctttttct tcaatcagtt
    33001 tatgggcagc tagacgctgt accgctcctg aaacctgaac tcctcgaatc tgctcgattt
    33061 cagctcttgt gattggctgt ttataagcga tgattgctaa ggtttcgacg gcggcttgag
    33121 ataatgtgtt tgatgtccct tgcgcatatc gcttaagtaa gggagccagc tcttttttag
    33181 tcgttaatac ataatggctt tccgtttcaa aaatatgcaa agctgagtgc gagttttgag
    33241 tgtaagattc ttttaattct ttcactaaac ttgtgacttc tgcttcgctg agatttaaca
    33301 gtccgcttaa ttcttcatta ctgatccctt cctcgccaac aataaaaaga agggcttcaa
    33361 gttcacttaa tttcgtcatg ttcggtatct cctactgctg gataaagcat aattgattca
    33421 tagttttctt tttgttgcgc aatgatctgc ccttttttca tcaattctag caaagccata
    33481 aaagtagtga caacttcact tttagaaggt gactcaaaaa aagaatcgaa agtacagcca
    33541 tgcgcatttc tttttatacg ctcttcgata aataccattc gttcttcgat cgttgtgtca
    33601 tcgggcgtga tcgttttttc cactttttga gaaaatttct tacgttctaa gattttctgc
    33661 atggcacgga tcaaatcgat ttttttgact tgtcctcgct tcaacggctt ttcagcatcc
    33721 gctaacccat cgatgttcat aggttctttt gtgtaataaa gacttcgttc ctctgctttt
    33781 tctgtcagtc tttcagctgc atatttatat ttacggtatt ctaaaagctg agtgaccaat
    33841 tcttctcgag gatcttcttc ccataattct tcatcgtctg tgacgaattc ttggacagga
    33901 agcaatgtct tgcttttgat tgccatcaat gtagcagcca tcactaagta ttctcctgca
    33961 acagctagct cgagtgtttt cattgtatgg atataattca tatattgctc cgtgatcgta
    34021 gcgatcggta tatcatatat atcgatttcc atttgctgaa tcagatgaag cagcagatcg
    34081 agaggaccct cgaatacatc taatttaata ttgatctccg tcaactcatt cactctttct
    34141 tctttttctt tgctagccat tttccaatgt aaggctctgt gatttcagtt gcacggatgg
    34201 agacatccgg gtacttttcc tgtaattcat ctaacatttg aaagcccata ttctcaccgc
    34261 gataagacgg attcaaagaa atgtcatgga tgatcagctg atcgttcgtt gcccagtaaa
    34321 taccaataat cccttgaatg ttggttgaac cttctggcgt aaagaaaaaa agttcataat
    34381 cgtcttcaga ttcgtacgta tcaatctcct tcaacaaaga agttttacct gtcagtttct
    34441 tatggaaact caatagtccc atagcaattt tacggttttc tttccggtaa tgtgttaaca
    34501 tcttctcacc ttcctatgac taagcccttg gaaaatgctg cttataaaca tccgtcattc
    34561 ttttcttcgt gatatgtgta taaatctgag tcgtagagat atccgcgtga cctaacaatt
    34621 cctgtactgt ccgcaaatca gctccatttt ctagcagatg tgtggcaaag ctatgccgca
    34681 gcgtatgtgg tgttacttct ttgtaaatcc cagcctctcg tactaactgc ttcagatttt
    34741 tccagattcc ttgccgagaa agacctttcc catgattatt gacaaataca tgcatttcag
    34801 atgcgtcctt tacaagtaat ggacgtgctt cgtctaagta gcgttccagc cattggatcg
    34861 cataatcacc taaaggaatg atccgctctt tatctccttt tccaactgtc tgtaccagtc
    34921 caagagaaag atgaagatct cccaatttca atccaatcaa ttcactgacg cgcatcccag
    34981 tagcatacat gacttccaag attgcacgat ctcttatccc taacgttttt gtcgtatcag
    35041 gtgcttcaat cagcttttct acttctgtca gagtcaatgt acttggtaat ttctgtgctt
    35101 tcttcggcgt atcgatatgt tgcatcggat catgatcagt gatacgctct tgccgtaaaa
    35161 actgatggaa tcttctgaga cttgagatca tccgagtaac agttgctgtc gctttttttt
    35221 cttcatgcaa cttctccaga aaacggatga ctaaaaagcg atcgacatcc tgccatgaag
    35281 caattttctg ctcctgtaga aaatgcaagt actgttccaa atcacgttca tagctttgtc
    35341 gtgtattggc ggacaatccc cgttcaatat tcaagtagtg gagataatcg ttgatttgct
    35401 ctttcataat aatccccctc atgcctctct atcatatcag ataccgcttc attctgatag
    35461 tctttttcca taatctgagc aaagaaaaag ccgtctagac actcagacgg actggtttct
    35521 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
    35581 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
    35641 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
    35701 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
    35761 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
    35821 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
    35881 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
    35941 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
    36001 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
    36061 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
    36121 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
    36181 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
    36241 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
    36301 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
    36361 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
    36421 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
    36481 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
    36541 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
    36601 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
    36661 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
    36721 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
    36781 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
    36841 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
    36901 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
    36961 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
    37021 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
    37081 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
    37141 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
    37201 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
    37261 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
    37321 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
    37381 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
    37441 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
    37501 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
    37561 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
    37621 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
    37681 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
    37741 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
    37801 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
    37861 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
    37921 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
    37981 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
    38041 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
    38101 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
    38161 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
    38221 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
    38281 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
    38341 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
    38401 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
    38461 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
    38521 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
    38581 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
    38641 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
    38701 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
    38761 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
    38821 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
    38881 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
    38941 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
    39001 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
    39061 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
    39121 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
    39181 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
    39241 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
    39301 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
    39361 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt
//
LOCUS       DACDIP010000017        39014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDIP010000017 DACDIP010000000
VERSION     DACDIP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.017"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV257_001102"
     CDS             6..305
                     /locus_tag="IV257_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8814248.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV257_001103"
     CDS             378..1739
                     /locus_tag="IV257_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8814249.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV257_001104"
     CDS             1761..2021
                     /locus_tag="IV257_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814250.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV257_001105"
     CDS             2035..2787
                     /locus_tag="IV257_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8814251.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV257_001106"
     CDS             complement(2881..3486)
                     /locus_tag="IV257_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8814252.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IV257_001107"
     CDS             complement(3539..4513)
                     /locus_tag="IV257_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8814253.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IV257_001108"
     CDS             complement(4891..5670)
                     /locus_tag="IV257_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8814254.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV257_001109"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV257_001109"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8814255.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IV257_001110"
     CDS             7161..8558
                     /locus_tag="IV257_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8814256.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IV257_001111"
     CDS             8639..9079
                     /locus_tag="IV257_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8814257.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IV257_001112"
     CDS             9227..9553
                     /locus_tag="IV257_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8814258.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IV257_001113"
     CDS             9554..10303
                     /locus_tag="IV257_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814259.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IV257_001114"
     CDS             complement(10383..10838)
                     /locus_tag="IV257_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8814260.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IV257_001115"
     CDS             complement(11265..11540)
                     /locus_tag="IV257_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814261.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IV257_001116"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IV257_001116"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8814262.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IV257_001117"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IV257_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8814263.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IV257_001118"
     CDS             14770..17436
                     /locus_tag="IV257_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8814264.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IV257_001119"
     CDS             17800..18552
                     /locus_tag="IV257_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8814265.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IV257_001120"
     CDS             complement(18574..19728)
                     /locus_tag="IV257_001120"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8814266.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="IV257_001121"
     CDS             19913..22354
                     /locus_tag="IV257_001121"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8814267.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="IV257_001122"
     CDS             complement(22684..23388)
                     /locus_tag="IV257_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814268.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="IV257_001123"
     CDS             complement(23575..23820)
                     /locus_tag="IV257_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814269.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="IV257_001124"
     CDS             complement(23837..24688)
                     /locus_tag="IV257_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8814270.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="IV257_001125"
     CDS             24854..25858
                     /locus_tag="IV257_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8814271.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26099..26455)
                     /locus_tag="IV257_001126"
     CDS             complement(26099..26455)
                     /locus_tag="IV257_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8814272.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26823..28010
                     /gene="tuf"
                     /locus_tag="IV257_001127"
     CDS             26823..28010
                     /gene="tuf"
                     /locus_tag="IV257_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8814273.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="IV257_001128"
     CDS             complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="IV257_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8814274.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30815..31534)
                     /locus_tag="IV257_001129"
     CDS             complement(30815..31534)
                     /locus_tag="IV257_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8814275.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            31743..32375
                     /locus_tag="IV257_001130"
     CDS             31743..32375
                     /locus_tag="IV257_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8814276.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            32434..33294
                     /locus_tag="IV257_001131"
     CDS             32434..33294
                     /locus_tag="IV257_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8814277.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            33401..35794
                     /locus_tag="IV257_001132"
     CDS             33401..35794
                     /locus_tag="IV257_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8814278.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            35976..37817
                     /locus_tag="IV257_001133"
     CDS             35976..37817
                     /locus_tag="IV257_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8814279.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            37841..38950
                     /gene="rpoD"
                     /locus_tag="IV257_001134"
     CDS             37841..38950
                     /gene="rpoD"
                     /locus_tag="IV257_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8814280.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT        12556 a         6864 c         8088 g        11506 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa agaacacttt
    25981 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt
    26041 ggagttaaac gcttctgtcc cagcctcttt ttatctaata aaattttaag accatttact
    26101 aattatcgat tttttctgcc gcatttttag gcacttcaga agcctttatt tcttcccagc
    26161 ttaatacacc tttacgagga ttgactttga gtttaagata cgcattcatc tttaatggat
    26221 gttctctttc ttcgcgaagc gtcacctgtt ttttttcacc atctttatta taagccgctt
    26281 gcttgtaggt ataagtcgtg aatttctctc cgttatctgc ttgggtagct gacatttcgc
    26341 cattcgtcgt gatttttgta taataagctt cgccgccgta aaaataatga taagctgcaa
    26401 aacttccgcc gagaaatatg cctaaaacga caagtacgcc aattaatttt ttcatgtact
    26461 tcacctcttt ccttattatt aacgaaatag aagatttcgt ctattgaaag ttgttggcaa
    26521 cagctgaatt ttaaactttt cctcattttg ttcttattgc ttcaaaagaa agttaaggac
    26581 tatcttgaag tttttctcaa aaaacacatt tttttattta taaaaggtac ttttttcgcg
    26641 tataagacag acgagtgctc ttagttgact ttgaccaata ttgacgtata ataagcttag
    26701 ttcgaatctt acaaaaagta agtcggacgt attctttttg atttagtcaa aaaaagacta
    26761 taataaaaag gtgagagctg aaatttttca gtgatctata ttatttagga ggtttgtaaa
    26821 ttatggcaaa agaacattat gacagaagta aaccacacgt taacattggt acgattggac
    26881 acgttgacca tgggaaaact actttaactg ctgcgatcac tacagtatta ggcaaaaaag
    26941 gcctagcaaa tcctcaagac tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg
    27001 ggattacgat caataccgca cacgtagaat atgaaacaga aaaacgccac tacgcgcata
    27061 ttgatgcacc aggacacgcg gactatgtga aaaacatgat tacaggggca gcacaaatgg
    27121 atggtgcaat cttagttgtt tctgcaactg acggtccgat gcctcaaaca cgtgaacaca
    27181 ttttattgtc acgccaagtt ggtgtaaaat acctgattgt tttcttgaac aaagttgatt
    27241 tagtcgatga tgaagaattg atcgatttgg tagaaatgga agttcgcgag ttattgagcg
    27301 aatatggttt cccaggcgat gacactcctg tgatcaaagg ttccgcatta aaagcattgc
    27361 aaggcgatcc agatgctgaa gctgctatta tggaattgat ggatacagta gatgaatata
    27421 tcccaacacc agaacgtgat acagataaac cattactatt gccagtggaa gacgtcttct
    27481 caattacagg tcgaggaact gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg
    27541 gtgatgaggt agagatcgta gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg
    27601 tagaaatgtt ccgtaaaacg ttagattacg gggaagctgg ggataacgta ggcgtgttgt
    27661 tacgggggat ccaacgtgac gatatcgaac gtggacaagt acttgctaaa ccaggttcca
    27721 ttactccaca tacaaaattc aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac
    27781 gtcatactcc attcttcaac aactatcgtc cacagttcta cttccgcaca actgatgtta
    27841 caggaacaat cacattgcca gaagatacag aaatggtcat gcctggtgac aacgtgacga
    27901 ttgatgttga cttgatccat ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg
    27961 aaggcggacg gacagtaggt tcaggtatcg ttacagagat cgaagcataa gaaacgcgac
    28021 tcgaatattt tggtaatgaa aaatgtttaa aataaaagag cggtccaaac aggatttctg
    28081 tttggaccgc tctttgttct tcaattgtat aagaagagat tgtagaagca tgaactttcg
    28141 ttttacttca gtctctttta ttcatcatga gagtgttcat ctatcggtat gcagttttta
    28201 atactttctt cgtatccatt ttttcaaacc acttattcta ccttgtgtct gctcaactgg
    28261 cgcattatca ataatcagca aagcaatcat aacatatgca atccaactat ctctagaata
    28321 tagagtataa cgtgagaccc agtttgtagc gtacttgctt ttatattctc ttaatccatg
    28381 gaaagaataa aaatgagaac caaactcgta aaccaagtat gcaatccttt cctgtaaaaa
    28441 actctttctg gaagttccga catttgaaag aggggccatc cctaaattga accattggat
    28501 atgttcttct ttcatatagg caaacaaatg caagaacaag aaatccatac tgccagaagg
    28561 tgctttttta gaatcatatc gcatcagatc aatcgttcct actttattgt tcgtgtaagt
    28621 aggcatgatc gttgcaaaag cgacaatttc ttcttgggta tttttgacaa ctgcaatagg
    28681 cgctctttgt agataatctt cagagaaaaa gcctaaagaa aacccttttt cctttcgtga
    28741 acccaaccaa ttatcagaga tatttttgaa agtactcatt tgctcagtag aaaatggtgg
    28801 ttgtaagaca tcaaatgtaa atccttctcg ttcgatccga ttcaagacag cacgagtgcc
    28861 cttcattttt ttccctgaag ttgtaaaact attcaggtct acataagctt cttcccccat
    28921 cttaatgaaa tcgtagccga attcatgcaa aatcataaca atctcttcgc ttgtttcgta
    28981 gaaaactggt aaatagcata gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg
    29041 aaaatcttct ttcttaccag aaggatcacc cattacaata catttattat tgaagcaagc
    29101 gaattggaga agaacagttg gttcaccgtc tttttcatag gcaaacatcc gcttatcttt
    29161 taagaagatc aattgactat cggagtttcc tccataagtt gtaaggatct tcagggcctt
    29221 ttcttcatta aaggcttcgc caatctgttt cttttctcct tgaaggaaat gaacaaatag
    29281 aacgatcatg aaactaacag cgatgatggc caacaatccc gaaaaccaaa tcttttcaga
    29341 aggaaataag aaaaaggaaa taaaatggtg acgccgatga gggaaatctg gcagattgta
    29401 taccccaata acgatgtaaa gcaaggacag tgcaccaata atgattccat cgatcgtacg
    29461 ccactcccat gaatagatca attgttcccg aaataattcg tttttagaag caagaatgat
    29521 cagaagcaga atagacaaga aaaccaccgg tgtgaaacta aaatcactta ataaaacata
    29581 aagtaaagct agaacaatca agaagattgt aggcaaatat gctcgtttca cacgtgctgc
    29641 gatccctctg cccataatga ttaaaagaaa tcccaatagt atactaggaa attggataat
    29701 aaaatgaaat ttcaatgggt tcaaactatg aagccagcga aactctgtaa aagcttgcgg
    29761 gatagtggca gaaagtacta gcatgattcc agaaaagtat aataagacaa ccacgatttt
    29821 atgcgcaatt tctgttgcta attgtttagg gataccagaa taacgctgat caaaagtgga
    29881 accgatattt ttgaagaaaa agacgattcc aatcaaaaac ggaatgatgt agtagaacag
    29941 gcgatagagc aagatccaca caacgactat ttctcttggc acaccaatag cggacaaccc
    30001 gataatcatc attacatcga aactcccaat ttctcctgga atcatagaca cgattcctat
    30061 cactgaagca gcaatgaata atggaattgt ttgccacaga tcaatccgga tttccattaa
    30121 ataaccaaca gaaaggaaac taaacaggac accggtccat tccaacaatg aaactagcaa
    30181 tagctgtcct tgagttttaa gtgaggtacc accaaggaat cctttctttt tgaaagtagt
    30241 aaataaaaag acgacaggaa aatatagacc gccgccgatc aaccatatcc aatattgctt
    30301 caaaaaagga gcaactggcg tgaagactac aagtaaaaaa gaaagcagac tgtaaataga
    30361 aagtccagcc atcaaaaaca agaaaacttt cgataatgct tggatcacgt ttctgctctc
    30421 tttcttttgt ccatatagtt cagagcgaag ccccacacta acaaacccgc cgaaaccggc
    30481 aatattgttg atcgtattga tcagccagct tgtctcaaac aggtaacgag gattctgctt
    30541 ttgttcaagc atccgattca aaataatatc ataaccaacc ataggtaaca cagagataag
    30601 accaatcaat agcattagtc cgcttttcca tacaggtata gtggcaaacg tttctcctaa
    30661 ttgctggata gatagagttt taccgatgga catcaattca ccgacaataa tgacaatgac
    30721 agaaatcaaa aagattgttt tgaacagtcc taggtggttt ttcagccatt gtatggtatt
    30781 ttttaacaaa taaatcacct tctattccta atagttatgt ttcagaaaga aattgaagaa
    30841 cttctcgatt gaagcgttca ggatcttttc tagctaattc atgtccttgt tctttcacaa
    30901 gcacaaagga agcttttggt atagttttag ctatatacaa agaatgtttt agcttgatta
    30961 catctttttt tcccacgatg atcaaagtgg gggagttgat tttttttaaa tcattttctg
    31021 taagcccgat gtcatgcagc aaaagtttga taactaaaag atttttgcga agacttgggc
    31081 gaaataaaga gagtaaccat acccatgcat aatgaatatt actgatgact ctggcagaaa
    31141 aacgaacgcc tttaacaagc gtgtttccag aatttaaaat caatcgatga acttttttag
    31201 ggaagctgct ggcaaatacc agtgctagat ttgctccatc actaaatcca agaaaatctg
    31261 cctgatcgat tttttccagc aacatgatcg tattcagatc ctcggccatt aactgaaaat
    31321 tcaacataga tgcttcgttt gtcgaacgac catgtcctcg gctatctact agatacacgg
    31381 tatagtagcg ttcgagtaca gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc
    31441 catgcagtaa aaagagaggg aagccttgac cgctcttttc atagtaaatt tcgctcccat
    31501 ctggcatttt tgcatatttc ttttcttttt tcacataatc acctactgtg taaagtataa
    31561 cgattatatc agtcattggg caattgtttt tactgaaaaa caacgatcat tttcgtaaga
    31621 tgtcaaaaaa tacaggaaaa ttcatttttt cttaaggcgt acacagaaat caaggaagaa
    31681 atagccaaga agataaagaa atagtaagat aaaaaaagat gtcaaaataa ctaggtgatg
    31741 aaatgaagcg aaaacgatta tctattatcc gtattctttt ttacatgatc attctgacgg
    31801 ttattggtgc ggtgttgttt ttaggaggaa gaacttatcg ttcggtaaaa cgagtggaaa
    31861 gctatcaaat agaaataaca acagctgcaa aaaattatca tatggaaaac tacgagaatt
    31921 tgatcaaggc gataatattg accgaatcaa aaggaaaagg gacagatctc atgcagagct
    31981 cggaaagtct gcatggagaa gctgagatca ttgataatcc agctgacagt attgatcaag
    32041 gtgtcaaata tttggcggaa atgatagtag aagcaaagaa acaaggatgc gatttatgga
    32101 cagctgtcca agcatataat tttgggaaag attatattgc ctatgtaaaa aatcatggcg
    32161 gagaaaacac agtagaatta gcagaagcgt attctaaaga tgtgctgagc ccacttttag
    32221 gaaatgagga agggaaacaa taccgatatt ggcggatcca gtccttgctt tacaatggcg
    32281 gctatctcta tcataatgga ggtaacatgt tttatcctga tattgtcaaa atgaatgaaa
    32341 aaataataga gacctatgaa aaagtcttta agtaaaatag caggaaatgt tcaatcatga
    32401 ataaaattaa atgaaagaag gagtgcgcaa atagtggaaa aaaggcaaaa aatggaaaat
    32461 caagaagtgg ggtacacaac caaagactgg atgttacggt ttgtgaaggg aatgtttatc
    32521 ggttcaggat ttatcttgcc aggagtcagc ggtggtgcat tagcagctgt ctttggcatg
    32581 tatgagcggc tgatttcttt tttagcccac cttacaaaaa atttcaaaga aaatgttttg
    32641 ttttttctgc ctgttggtct aggcggagtg acaggcatct ttctactttc ttttttagtc
    32701 agttttttat taggtgcgta tgaaacaacg atattatggt tttttgtcgg atgtatcgct
    32761 ggaactgttc ctgcgttatg gaaagaagca gggaaaaaag gaagagatac ttctgatgtg
    32821 atcatcatgg ttgttacttt tgttctggct tatttgtttt tattatacgg cgctcaatta
    32881 tttgacgggc aagtcgatca gaattttgga acatggctga ttgccggcgg attgattggt
    32941 ttgggtatga tcgtcccagg attgagccct tctaattttt tggtgtatat gggattgtat
    33001 aaagcgatgg ctgacggatt taaagaagta cgacttgaag tgattgtgcc gatcggaatt
    33061 ggtgggattc tttgtgttct tggattgtca aaaatcatgg attacatttt ctcaaaggca
    33121 tatagcaagc tattccattt cattttaggt gtagtttttg cttcaacgat tatgatcatt
    33181 cctaccgatt acgcgggatt gggagttgct ggggtactag cttgtcctgt tctttttata
    33241 gccggtacag cactaggttg gtggatgagc cgtttggagg aacaatataa gtaaagatag
    33301 attgataata aaaacagcac acgggtgatt caaacaaaaa ggttggcatc ttttttgtaa
    33361 aatcattcgt gtgtttttct ataaatttta taaaaacaag ttgtttttaa tcttggaaca
    33421 cgctagactt aaacacgtga ttatttttga aagttggcgg aaaatggaga aaaagaaaat
    33481 gaatggactt acacagcaag aggtagttca gaaaatggct tctggccagc aaaatgacta
    33541 ccaggaagat gcatcaaaaa gtacaaaaca aatattcagc gataatatat tgacgttatt
    33601 caattttctg aactttggta ttgggatttg tctgttattt gtaggtgcgt attccaacat
    33661 ggcgtattta gcaattattg tcgtcaatat cgttattgga atttatcaag aaatacatgc
    33721 aagaaatctt gtacggaaat tatcgattgt ttcccaatca gaagtactag tgatccggga
    33781 cggtaaagaa caatcgattt ctactaaaga gctggtatta ggagatttgg taaaaatcgg
    33841 agcaggagaa cagataccag ccgatttaca ggttatatct ggacgggcag aagcaaatga
    33901 agctcttttg acaggcgaat ctgatttgat tgtcaaagaa actggagctg aattgctttc
    33961 tggaagcttt ttgacaagcg gttctgtcat ggctgaagtt atccgagtag gtgcagataa
    34021 ttatgctgtc cgcttgacta gcgaggcaaa gactcacaaa gctattcgtt ctgaacttgt
    34081 agattcgatt cgcaaagtat ccaaattcac tagtttcgtg attattcctt tggggatcat
    34141 actatttttg gaagcactgt tcatccgcga tgcttctatc aaaatttctg ttattgcatc
    34201 tgcagcagct ttgttaggca tgcttccaaa gggtttggta ttgctaataa gtattgcatt
    34261 aacgacaggg gtaaccaaac tggcaaaaaa acgaatcctt gttcaagaca tgtattcaat
    34321 tgaaacgctg gcccatgtcg atacattatg cttggataaa acagggacca ttactgaagg
    34381 gaaaatgagt atccagcggg tagaagcact acatccgctg tacgagaaag ccatcccaga
    34441 aattatgggg acgtatctgg cagaaagcag tgataacaac atcactatgc gggctatgag
    34501 agattatttt gagctttcta atcggtatga tgtgacaaac aaagtcgcat tttcttctga
    34561 tcgcaaatgg ggcgcgatgg aatttccaga actgggagtc gtgtacgtag gagcgcctga
    34621 acgtttggtg gaagaagatc ggcttccgga cagtctaaaa acagcccaag cgaatggcta
    34681 tcgcgtactt atgttgggga tcgctccaaa tcagcaattg aacgaagaaa aaccagcatt
    34741 tgttgagccg gttgctctgt ttgaaatcga tgatccaatc cgaaaaaacg ctcaagaaac
    34801 actagcttat cttcatgagg aaggtgtgga tctgaaagtg atttctggag acaatccttt
    34861 tactgtctcc aatatcgcta gacgtgcagg attaccgaat tatgatgcgt atgtcgatct
    34921 ttctcaaatt actgaagaaa tggaagtgcg agaagctgct catcgctata cggtttttgg
    34981 acgagtgagt ccgcagcaaa agaaattgct ggtaaatgaa ttgaaagaaa gcggcagaac
    35041 ggttgcaatg acaggcgacg gtgtgaatga tgttttggcg ttaagagaag cagattgcag
    35101 cattgctatg gcagaaggtg atggagcaac acgtcagatt gcgaatctgg tattgttgga
    35161 ctctgatttt actacattgc cagaagtcct gtttgaagga cgccgagtgg tcaacaatgt
    35221 gacgaaagtc tcaggcatct tttttatcaa aacgatttat tcatttattt tatcgattat
    35281 atgtgccgtt acagcaattg gatttccgtt tatccctatc caaatcacgc tgattgattt
    35341 agcgattgaa gggtatcctt ccttcttttt atcttttgaa caagataaaa gaaaaatcac
    35401 ttaccgttat ttgcctacag cacttattaa tgctttacca aatgccttgc ttgtagtagt
    35461 gaatatcatt gcggtgtatc tacttggaaa aacattcgca tggggaaatg ctgaaactac
    35521 tactttgatg tattacttgc tgattgggat cagttgcatg gcagttgtaa aagcttgtta
    35581 tcccttcaac ccattgcgca tcttcctagc agtaacgacg atcgtaggga tatatgtagc
    35641 tgcaatgctg ttccatggaa ttttggaagt aaacttaggg ttgggagaga gcgtaggata
    35701 cttctttgta ttcatgctcg taaatattgt tcttcgagta ttgtactgga aggctgatat
    35761 tcaaaatcag ttactgaaaa gagtagcaaa ttgatgctta atgagataga aatgtgttat
    35821 acttgaaaaa gcaatgaaat atgatacaat caaacatgta taattgtgtc ctttaaaatc
    35881 gcactgattt cagttagtgc gattttttac cggttattct cctgaaatat cttctgttaa
    35941 acccaaaaga tatcaataga gcagggggtg aaaaaatggc tcaacggatt cctcaagagg
    36001 ttatcgaaac agtacgcagt caaacgaata tcgttgaggt gattggacaa tatgtccagt
    36061 tgaaaaagtc tggtaaaaat tacttggggc tgtgtccttt tcatgaagaa cgtacaccgt
    36121 ctttttctgt tgcagaagac aagcagattt ttcattgctt tggttgtggt aaaggcggaa
    36181 acgtgtttac ttttcttcaa gaactagaag gtctaagttt tccagaagca gtggtaaaag
    36241 tggcagactt cgaacagata ccgttagaag atcaatggcg tcagcgtact atagctgtcc
    36301 aaaatcctga atctgctacg gggaaactga ttgctgctca tgaaaaagca gcggaaatct
    36361 atcatcacat gctattgcat acaaaagctg gacagccagc tttagaatat ttgcaaagtc
    36421 gtgggttgtc tttagaatta attgaagaat tcaacatagg ttttgcacct aatgagcgtt
    36481 cttttcttca gcaagtattc aagaatgaat caatttcaaa tgaattgtta gaacgctctg
    36541 gattgtttgt cacgcatgat gatggccgta tgtctgatcg tttttatcaa agagtgatgt
    36601 ttccacttcg taattctcaa ggccagacga tcggtttttc aggacgctgg cttgcacctg
    36661 aaaaagggca ggaaaaagat cagcccaaat acttgaatag tccagaaaca gaactgttca
    36721 ataaaagaca agttttattt aatcttgata aagcaagaag cgatatccgg aaaaacggag
    36781 aaattttgtt gttcgaaggg tttatggatg tgattgctgc atggcaggct ggtatcaaaa
    36841 ctggattagc ttctatgggg acaagtctaa cagctcaaca gatccaacaa atggaaaagc
    36901 tgacgaaaga attggtcttt tgctatgacg gagacaacgc aggttttgaa gcaaccaatc
    36961 gagggattga attgcttcgg caaaacagtc gattagcact ttctgtcgta gtcgttccag
    37021 aaaagcttga tcctgatgaa tatctccgta agtatggaga agcatctttc caagaattag
    37081 tggctcatgg gcgcgaaaca gtctttactt ttaaatcacg ttatttaaaa caagggaaaa
    37141 atttagataa tgaaaaagac aagatcgaat acctcgaaga attactagtt gaacttagcc
    37201 gagttgtttc accaatcgaa caagatatgt atctatctca actgtcaaca gagtttcaga
    37261 tcggcagaga gacgatacaa aaacaactgc gcgatcttcg aaggacaaat cagcaaaatc
    37321 gtcaagaacc tcaggaaagt caacacactc aggttacgag acaagaaact gcacggaaaa
    37381 agagaccttt gacgcaagta gaaaaggcag agcagattct attatatcgt gcatttaaag
    37441 aagtttctgt ccgtaatatt ttaaaagaac gagaatttca gtttattcat gatgcatatg
    37501 cagaagttta ttttttattc gatgcatatg tctcacaaca ttatgaattc aaccttgcag
    37561 agtttttaga ctttttgcaa gatgaaaatt taaggcggtt ggtggtggaa atcgaatatt
    37621 taccggtggc agaagaaagt tcgccacgag aaatccaaga cttgctagat gtgatcagca
    37681 aatctggctt agctgatgaa ataacattaa aaaaacaaat gcagcaagaa gccagacgaa
    37741 ctggaaacaa acagcttgag cttgaattga cgattgaaat catcaattta acgaaacagt
    37801 taaaacaagc caaatagcta ctcaaggggg gccttctttt atggcaaatg gaacagataa
    37861 aaaaacgtat gaagcagcag tagcagcttt catcaaagaa aacaaaccca aacgacaagt
    37921 tgtatatgat gatttatcca atcagcttgc tacaccattc actttgaatg cagatgaaat
    37981 ggacaaattg atccaaaaga ttgaagacgc aggtatcagt gtggtggatg aaaatggtga
    38041 tccatcaatt cacagcttga agagtgctga gaaaaaagct gaacaagcaa aaacagaaga
    38101 tctttctgcg ccaaccggtg taaaaatcaa tgacccggtt cgtatgtatc tgaaagaaat
    38161 tggccgtgtc tcccttttaa ctgcggaaga agaagtagcg ttagcattga aaatcgaaga
    38221 aggcgaccaa gaagcgaaac agcgtctggc agaagcaaac cttcgtttag tagtaagtat
    38281 cgctaaacgt tatgtaggac gaggcatgca gttccttgac ttgatccaag aaggaaacat
    38341 gggactgatg aaggcagttg aaaaatttga ctaccgcaaa ggatttaagt tttcaaccta
    38401 tgctacatgg tggattcgtc aagccatcac tcgagcaatc gctgaccaag cacgtacgat
    38461 ccgtattcca gttcatatgg ttgaaacaat taataaattg atacgtatcc aacgtcaatt
    38521 gctgcaagac cttggaagag aacctacgcc agaagaaatc ggtgcggaaa tggatcttcc
    38581 aacagaaaaa gtccgagaaa tcttgaaaat cgcgcaagaa cctgtttccc ttgaaacgcc
    38641 aatcggtgaa gaagatgact ctcacttagg tgatttcata gaagaccagg aagcaacaag
    38701 tccagcagaa cacgcagctt atgagttact aaaagaacaa ttggaagatg tactagatac
    38761 attgactgat cgggaagaga atgtccttcg cttacgtttt ggtttagatg atggccggac
    38821 gagaacattg gaagaagtag ggaaagtatt tggtgttact cgggaacgta ttcgtcagat
    38881 cgaggctaaa gcattaagaa aattgcgcca tccttctcgt tcaaaacaat taaaagattt
    38941 cttagaataa gcgtggacta ttgaccagaa gagaaatcag taaaactgtt tggtttattt
    39001 taaaagaaaa aaag
//
LOCUS       DACDIP010000018        38489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDIP010000018 DACDIP010000000
VERSION     DACDIP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.018"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            33..656
                     /locus_tag="IV257_001135"
     CDS             33..656
                     /locus_tag="IV257_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814281.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="IV257_001136"
     CDS             1106..2155
                     /locus_tag="IV257_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8814282.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="IV257_001137"
     CDS             2152..3033
                     /locus_tag="IV257_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814283.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="IV257_001138"
     CDS             3036..3806
                     /locus_tag="IV257_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8814284.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="IV257_001139"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="IV257_001139"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8814285.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="IV257_001140"
     CDS             5117..6280
                     /locus_tag="IV257_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8814286.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="IV257_001141"
     CDS             6397..6807
                     /locus_tag="IV257_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8814287.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="IV257_001142"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="IV257_001142"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8814288.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9347..9664
                     /locus_tag="IV257_001143"
     CDS             9347..9664
                     /locus_tag="IV257_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814289.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9824..10060
                     /locus_tag="IV257_001144"
     CDS             9824..10060
                     /locus_tag="IV257_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8814290.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="IV257_001145"
     CDS             10057..10455
                     /locus_tag="IV257_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8814291.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11122..12066)
                     /locus_tag="IV257_001146"
     CDS             complement(11122..12066)
                     /locus_tag="IV257_001146"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8814292.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12362..13306)
                     /locus_tag="IV257_001147"
     CDS             complement(12362..13306)
                     /locus_tag="IV257_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8814293.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13527..14741
                     /locus_tag="IV257_001148"
     CDS             13527..14741
                     /locus_tag="IV257_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8814294.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14763..16778
                     /locus_tag="IV257_001149"
     CDS             14763..16778
                     /locus_tag="IV257_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8814295.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16861..18234)
                     /locus_tag="IV257_001150"
     CDS             complement(16861..18234)
                     /locus_tag="IV257_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8814296.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18260..18544)
                     /locus_tag="IV257_001151"
     CDS             complement(18260..18544)
                     /locus_tag="IV257_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8814297.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18546..20561)
                     /locus_tag="IV257_001152"
     CDS             complement(18546..20561)
                     /locus_tag="IV257_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8814298.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20740..21045)
                     /locus_tag="IV257_001153"
     CDS             complement(20740..21045)
                     /locus_tag="IV257_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8814299.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21134..22165)
                     /locus_tag="IV257_001154"
     CDS             complement(21134..22165)
                     /locus_tag="IV257_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8814300.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22372..23247
                     /locus_tag="IV257_001155"
     CDS             22372..23247
                     /locus_tag="IV257_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8814301.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23399..24241)
                     /locus_tag="IV257_001156"
     CDS             complement(23399..24241)
                     /locus_tag="IV257_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8814302.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24333..26210)
                     /locus_tag="IV257_001157"
     CDS             complement(24333..26210)
                     /locus_tag="IV257_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8814303.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26207..27628)
                     /locus_tag="IV257_001158"
     CDS             complement(26207..27628)
                     /locus_tag="IV257_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8814304.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27631..31020)
                     /locus_tag="IV257_001159"
     CDS             complement(27631..31020)
                     /locus_tag="IV257_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8814305.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31277..32668
                     /locus_tag="IV257_001160"
     CDS             31277..32668
                     /locus_tag="IV257_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8814306.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32741..33388)
                     /locus_tag="IV257_001161"
     CDS             complement(32741..33388)
                     /locus_tag="IV257_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8814307.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33396..34082)
                     /locus_tag="IV257_001162"
     CDS             complement(33396..34082)
                     /locus_tag="IV257_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814308.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34088..35161)
                     /locus_tag="IV257_001163"
     CDS             complement(34088..35161)
                     /locus_tag="IV257_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8814309.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35383..36057
                     /locus_tag="IV257_001164"
     CDS             35383..36057
                     /locus_tag="IV257_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8814310.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36062..37402
                     /locus_tag="IV257_001165"
     CDS             36062..37402
                     /locus_tag="IV257_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8814311.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37551..>38489)
                     /locus_tag="IV257_001166"
     CDS             complement(37551..>38489)
                     /locus_tag="IV257_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8814312.1"
                     /translation="EALSVIERLRNEGYERDDIVIYTTDEAASRLGFDGLSGIDVETD
                     ENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRGDISDGKFVITVKGYRQ
                     PETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQNTTDPLNMTPGTDPMD
                     VPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVTTGEVDIHKHVVNDTET
                     VEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQVDVSKHTVIREEVGIH
                     KEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11852 a         7023 c         7295 g        12319 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt
    11101 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
    11161 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
    11221 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
    11281 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
    11341 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
    11401 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
    11461 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
    11521 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
    11581 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
    11641 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
    11701 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
    11761 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
    11821 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
    11881 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
    11941 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
    12001 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
    12061 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
    12121 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
    12181 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
    12241 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
    12301 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
    12361 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
    12421 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
    12481 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
    12541 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
    12601 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
    12661 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
    12721 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
    12781 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
    12841 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
    12901 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
    12961 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
    13021 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
    13081 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
    13141 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
    13201 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
    13261 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
    13321 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
    13381 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
    13441 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
    13501 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
    13561 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
    13621 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
    13681 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
    13741 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
    13801 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
    13861 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
    13921 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
    13981 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
    14041 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
    14101 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
    14161 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
    14221 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
    14281 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
    14341 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
    14401 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
    14461 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
    14521 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
    14581 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
    14641 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
    14701 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
    14761 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
    14821 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
    14881 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
    14941 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
    15001 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
    15061 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
    15121 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
    15181 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
    15241 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
    15301 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
    15361 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
    15421 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
    15481 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
    15541 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
    15601 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
    15661 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
    15721 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
    15781 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
    15841 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
    15901 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
    15961 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
    16021 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
    16081 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
    16141 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
    16201 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
    16261 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
    16321 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
    16381 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
    16441 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
    16501 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
    16561 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
    16621 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
    16681 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
    16741 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
    16801 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
    16861 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
    16921 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
    16981 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
    17041 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
    17101 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
    17161 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
    17221 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
    17281 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
    17341 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
    17401 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
    17461 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
    17521 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
    17581 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
    17641 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
    17701 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
    17761 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
    17821 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
    17881 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
    17941 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
    18001 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
    18061 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
    18121 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
    18181 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
    18241 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
    18301 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
    18361 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
    18421 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
    18481 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
    18541 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
    18601 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
    18661 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
    18721 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
    18781 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
    18841 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
    18901 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
    18961 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
    19021 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
    19081 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
    19141 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
    19201 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
    19261 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
    19321 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
    19381 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
    19441 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
    19501 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
    19561 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
    19621 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
    19681 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
    19741 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
    19801 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
    19861 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
    19921 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
    19981 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
    20041 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
    20101 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
    20161 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
    20221 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
    20281 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
    20341 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
    20401 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
    20461 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
    20521 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
    20581 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
    20641 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
    20701 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
    20761 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
    20821 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
    20881 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
    20941 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
    21001 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
    21061 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    21121 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    21181 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    21241 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    21301 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    21361 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    21421 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    21481 tgatgcggaa ataactagat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    21541 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    21601 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    21661 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    21721 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    21781 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    21841 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    21901 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    21961 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    22021 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    22081 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    22141 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    22201 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    22261 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    22321 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    22381 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    22441 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    22501 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    22561 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    22621 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    22681 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    22741 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    22801 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    22861 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    22921 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    22981 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    23041 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    23101 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    23161 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    23221 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    23281 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    23341 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    23401 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    23461 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    23521 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    23581 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    23641 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    23701 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    23761 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    23821 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    23881 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    23941 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    24001 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    24061 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    24121 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    24181 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    24241 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    24301 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    24361 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    24421 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    24481 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    24541 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    24601 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    24661 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    24721 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    24781 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    24841 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    24901 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    24961 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    25021 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    25081 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    25141 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    25201 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    25261 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    25321 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    25381 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    25441 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    25501 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    25561 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    25621 tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta
    25681 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    25741 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    25801 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    25861 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    25921 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    25981 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    26041 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    26101 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    26161 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    26221 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    26281 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    26341 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    26401 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    26461 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    26521 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    26581 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    26641 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    26701 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    26761 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    26821 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    26881 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    26941 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    27001 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    27061 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    27121 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    27181 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    27241 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    27301 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    27361 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    27421 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    27481 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    27541 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    27601 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    27661 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    27721 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    27781 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    27841 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    27901 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    27961 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    28021 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    28081 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    28141 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    28201 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    28261 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    28321 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    28381 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    28441 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    28501 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    28561 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    28621 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    28681 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    28741 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    28801 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    28861 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    28921 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    28981 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    29041 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    29101 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    29161 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    29221 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    29281 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    29341 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    29401 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    29461 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    29521 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    29581 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    29641 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    29701 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    29761 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    29821 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    29881 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    29941 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    30001 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    30061 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    30121 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    30181 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    30241 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    30301 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    30361 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    30421 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    30481 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    30541 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    30601 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    30661 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    30721 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    30781 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    30841 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    30901 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    30961 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    31021 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    31081 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    31141 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    31201 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    31261 atgaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    31321 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    31381 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    31441 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    31501 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    31561 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    31621 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    31681 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    31741 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    31801 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    31861 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    31921 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    31981 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    32041 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
    32101 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
    32161 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
    32221 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
    32281 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
    32341 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
    32401 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
    32461 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
    32521 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
    32581 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
    32641 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    32701 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    32761 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    32821 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
    32881 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
    32941 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    33001 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    33061 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    33121 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    33181 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
    33241 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    33301 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    33361 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    33421 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    33481 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    33541 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    33601 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    33661 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    33721 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    33781 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
    33841 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
    33901 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    33961 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    34021 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    34081 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    34141 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
    34201 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    34261 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    34321 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    34381 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
    34441 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    34501 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    34561 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    34621 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    34681 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    34741 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
    34801 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    34861 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    34921 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    34981 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    35041 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    35101 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    35161 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
    35221 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
    35281 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
    35341 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
    35401 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
    35461 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
    35521 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
    35581 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
    35641 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
    35701 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
    35761 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
    35821 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
    35881 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
    35941 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
    36001 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
    36061 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
    36121 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
    36181 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
    36241 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
    36301 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
    36361 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
    36421 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
    36481 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
    36541 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
    36601 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
    36661 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
    36721 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
    36781 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
    36841 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
    36901 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
    36961 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
    37021 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
    37081 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
    37141 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
    37201 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
    37261 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
    37321 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
    37381 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
    37441 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
    37501 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt
    37561 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
    37621 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
    37681 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
    37741 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
    37801 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
    37861 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
    37921 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
    37981 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
    38041 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
    38101 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
    38161 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
    38221 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
    38281 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
    38341 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
    38401 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
    38461 cgcaaccgtt caattactga taatgcttc
//
LOCUS       DACDIP010000019        37900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDIP010000019 DACDIP010000000
VERSION     DACDIP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.019"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            64..765
                     /locus_tag="IV257_001167"
     CDS             64..765
                     /locus_tag="IV257_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8814313.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            762..1883
                     /locus_tag="IV257_001168"
     CDS             762..1883
                     /locus_tag="IV257_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8814314.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1898..3130
                     /gene="pepT"
                     /locus_tag="IV257_001169"
     CDS             1898..3130
                     /gene="pepT"
                     /locus_tag="IV257_001169"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8814315.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3262..4170
                     /locus_tag="IV257_001170"
     CDS             3262..4170
                     /locus_tag="IV257_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8814316.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4205..4477
                     /locus_tag="IV257_001171"
     CDS             4205..4477
                     /locus_tag="IV257_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8814317.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4488..5534
                     /locus_tag="IV257_001172"
     CDS             4488..5534
                     /locus_tag="IV257_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8814318.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5785..8394
                     /gene="clpB"
                     /locus_tag="IV257_001173"
     CDS             5785..8394
                     /gene="clpB"
                     /locus_tag="IV257_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8814319.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8545..9216)
                     /locus_tag="IV257_001174"
     CDS             complement(8545..9216)
                     /locus_tag="IV257_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814320.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9209..9703)
                     /locus_tag="IV257_001175"
     CDS             complement(9209..9703)
                     /locus_tag="IV257_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8814321.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9723..10943)
                     /locus_tag="IV257_001176"
     CDS             complement(9723..10943)
                     /locus_tag="IV257_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8814322.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11090..12925
                     /gene="lepA"
                     /locus_tag="IV257_001177"
     CDS             11090..12925
                     /gene="lepA"
                     /locus_tag="IV257_001177"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8814323.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13121..14032
                     /locus_tag="IV257_001178"
     CDS             13121..14032
                     /locus_tag="IV257_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8814324.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15028..15279
                     /locus_tag="IV257_001179"
     CDS             15028..15279
                     /locus_tag="IV257_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814325.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15362..15634
                     /locus_tag="IV257_001180"
     CDS             15362..15634
                     /locus_tag="IV257_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814326.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            15724..15963
                     /locus_tag="IV257_001181"
     CDS             15724..15963
                     /locus_tag="IV257_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814327.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            16110..20393
                     /locus_tag="IV257_001182"
     CDS             16110..20393
                     /locus_tag="IV257_001182"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP8814328.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            20492..20857
                     /locus_tag="IV257_001183"
     CDS             20492..20857
                     /locus_tag="IV257_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814329.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            20859..21311
                     /locus_tag="IV257_001184"
     CDS             20859..21311
                     /locus_tag="IV257_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814330.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            21925..23235
                     /locus_tag="IV257_001185"
     CDS             21925..23235
                     /locus_tag="IV257_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8814331.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            23258..23569
                     /locus_tag="IV257_001186"
     CDS             23258..23569
                     /locus_tag="IV257_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814332.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            23930..25186
                     /locus_tag="IV257_001187"
     CDS             23930..25186
                     /locus_tag="IV257_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8814333.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            25183..25728
                     /locus_tag="IV257_001188"
     CDS             25183..25728
                     /locus_tag="IV257_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814334.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            25729..26229
                     /locus_tag="IV257_001189"
     CDS             25729..26229
                     /locus_tag="IV257_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814335.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            26310..26866
                     /locus_tag="IV257_001190"
                     /pseudo
     CDS             26310..26866
                     /locus_tag="IV257_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26886..27230
                     /locus_tag="IV257_001191"
     CDS             26886..27230
                     /locus_tag="IV257_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814336.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            27249..28160
                     /locus_tag="IV257_001192"
     CDS             27249..28160
                     /locus_tag="IV257_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8814337.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            28239..29231
                     /locus_tag="IV257_001193"
     CDS             28239..29231
                     /locus_tag="IV257_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8814338.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            29228..29446
                     /locus_tag="IV257_001194"
     CDS             29228..29446
                     /locus_tag="IV257_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814339.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            29459..29869
                     /locus_tag="IV257_001195"
     CDS             29459..29869
                     /locus_tag="IV257_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814340.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            29921..32449
                     /locus_tag="IV257_001196"
     CDS             29921..32449
                     /locus_tag="IV257_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8814341.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            32465..34675
                     /locus_tag="IV257_001197"
     CDS             32465..34675
                     /locus_tag="IV257_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814342.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            34683..35774
                     /locus_tag="IV257_001198"
     CDS             34683..35774
                     /locus_tag="IV257_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8814343.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            35795..36337
                     /locus_tag="IV257_001199"
     CDS             35795..36337
                     /locus_tag="IV257_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814344.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            36559..36789
                     /locus_tag="IV257_001200"
     CDS             36559..36789
                     /locus_tag="IV257_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814345.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRANWNGVVDSVITGSMDSALHGFTGH"
     gene            36803..37135
                     /locus_tag="IV257_001201"
     CDS             36803..37135
                     /locus_tag="IV257_001201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814346.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            37180..37728
                     /locus_tag="IV257_001202"
     CDS             37180..37728
                     /locus_tag="IV257_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8814347.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT        12857 a         5973 c         8078 g        10992 t
ORIGIN      
        1 ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag aaggagtgaa
       61 gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt tgtccctgaa
      121 tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc attgatatta
      181 agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta tgaatcagca
      241 cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct tgcaaatggt
      301 ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg tatgggaggt
      361 tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag cggacaagaa
      421 cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct gcaagaaaat
      481 cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata tgaaatcatt
      541 gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat gttcgggcca
      601 tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa
      661 agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa
      721 atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc agcggacgca
      781 ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa ggagacccag
      841 tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg acattagacg
      901 ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg attgctaaac
      961 atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa gaaaaaatgt
     1021 atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac atggatatta
     1081 tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg gagagttatt
     1141 tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct gtggatcatg
     1201 cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt gattataccg
     1261 gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt gcacaaccag
     1321 cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag gttattttgc
     1381 cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct tatgaggaac
     1441 ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta ggacgagtag
     1501 gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa gaagcgttcc
     1561 aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg
     1621 cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa agagcagatg
     1681 tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc gctgggttga
     1741 ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt atcgagaaat
     1801 ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct gaaacaaata
     1861 caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc ttttgccacg
     1921 ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa cgactccatc
     1981 gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag agttaggctt
     2041 agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac caagcaatgt
     2101 tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag attttaatgc
     2161 agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga tcgtactaga
     2221 tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa attatgaagg
     2281 ccacacattg atcacaacag atggcagtac attactggga gcagatgaca aatcagggat
     2341 tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc ctcacggtac
     2401 catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata aattcgatgt
     2461 tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag gtgagttgca
     2521 gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa atgtacatcc
     2581 agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt ttcataatca
     2641 gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct tccatttgat
     2701 ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg atcatgatcg
     2761 tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt tgaatcagcg
     2821 atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca tgaaagaaat
     2881 cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat
     2941 ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct cttacatggg
     3001 tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat tcgaatttgt
     3061 ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag aattgaacac
     3121 aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg
     3181 tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc tcttttttga
     3241 agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa aagttcaggg
     3301 aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga aaagcagcca
     3361 gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga attagtcaag
     3421 aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg cacgattcat
     3481 catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt acttccaggt
     3541 ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga agaagctgct
     3601 gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc
     3661 attatctcta cactgacaat cggaatctta gcagatacag cagtttggat cagtcaagaa
     3721 gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt attgctgaag
     3781 aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg aaaatcccaa
     3841 ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga cgactcagcg
     3901 accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat tttccgattt
     3961 attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg cattacttat
     4021 ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac tcgaacggat
     4081 ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg attaacagtt
     4141 tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag ttggaagaag
     4201 agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg tgaattttta
     4261 cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct ggcgtgttcc
     4321 tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat tgatctctcg
     4381 aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt
     4441 acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg tcgtctaaag
     4501 gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt gccattattt
     4561 attatttaat caatcaatca tttgcagata tctttgccga attgctgtct accagtgttg
     4621 ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa gggcgctcaa
     4681 tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg ttcttgacct
     4741 ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg atctcggaga
     4801 tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg acagcactcc
     4861 atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg gtcatccaat
     4921 tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct ggtatccttt
     4981 tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat gttcaaatat
     5041 tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa tgggtggata
     5101 catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc caagacagga
     5161 ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg tatgtacttc
     5221 cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc atttcgttta
     5281 tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt ggttcctttg
     5341 aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca gtttcttcgc
     5401 ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt gtgtatgtga
     5461 ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa gagaataaag
     5521 aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa gaaaaacgtt
     5581 atggataaga taattttacg tttgtttggc tcttttttta tatacataca atcaattttg
     5641 tttttttaag atttatataa gtcgagaaaa aaagtcaaaa aactatgtgt atattttgac
     5701 caaaattgac caaagcgata taatagtttt tgtaaagatc aaagaaataa gtgatcttac
     5761 ctttaattag gagggtatac ctgtatgaat attgagaaaa tgactacgac gcttcaagaa
     5821 gcgattgctg aagctcaaca gattgcggtc actcgaaaac atcaagatat cgacattgcc
     5881 catgtatgga aaatcttttt acaatctaat cattttggtc gaaatttcta tacggatgcc
     5941 ggattggatg tcgaatcttt tgaacatgaa attgatcgtc tactggatga gtatccagtt
     6001 gtttctggtg gaaacgtcca atatggacaa aacctcagcc agaatttctt tcgattattg
     6061 aatgaagcag accaaatacg tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt
     6121 attttagcgt tgatgaaatt aaaaaattat ccgttaactg tttatttgaa taaaaacgga
     6181 ttatctgaaa aagaattgcg taaaaacatc gaagagatga gaggaggaga aagagtgacc
     6241 tcacaaaacc aagaagaaca atacaaagca ttagaaaaat acggcgttga tttagtacag
     6301 caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc
     6361 atccgtattc tttcacgaaa aacaaaaaat aatcctgttc tgataggaga acccggtgta
     6421 gggaaaacgg caatcgttga agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa
     6481 aatttaaaag ataaaacgat tttttcatta gatatgggtg cattgatcgc tggagcaaaa
     6541 ttccgtggtg aatttgaaga aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat
     6601 ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg taggggcagg aaaaacagaa
     6661 ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga attacatttg
     6721 atcggtgcca caactttaga tgagtaccgt caatatatgg aaaaagacaa agcgttggaa
     6781 cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat cagtattctg
     6841 cgtggattaa aagaacgttt tgagatccac catggtgtta acatccatga caacgcatta
     6901 gtagcagcag ctactttatc tgaccgttat attactgatc ggttcttacc ggacaaagca
     6961 atcgatttga tcgatgaagc cagtgcaacg attcgggtag aaatgaactc tatgccaacg
     7021 gaattagatc aagttactag acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa
     7081 aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa
     7141 cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat
     7201 tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga agatgcagaa
     7261 aataactatg atttagaacg cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa
     7321 aaagaattga aggaattaga agctaaagca aaagacagcg agattaagat ggttcaagag
     7381 tccgttaccg aaaatgagat tgctcaagtc gttggcagat taacgggtat ccccgtaacg
     7441 aaacttgtag aaggtgaacg agaaaaacta attaaattga atgaaacact tcataaacga
     7501 gtgattggtc aagatgaagc agttgatgcg gtcagtgacg cggtaattcg ttcacgggct
     7561 ggattgcaag acccaaatcg tcctctaggt tctttccttt tcctaggacc aactggtgtc
     7621 ggaaaaaccg aattagcgaa agcgttagct gagaacttgt ttgactcaga agatcatatg
     7681 gtacggatcg atatgagtga atatatggaa aaacatgctg tgtctcgtct agtcggagct
     7741 cctccaggct atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat
     7801 ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc
     7861 ttgttacaag tcctagatga cggacgcttg actgattcaa aagggcgagt agtagatttc
     7921 aaaaatacgg ttctgatcat gaccagcaat atcggttcac agttactgct ggaaggcgtg
     7981 actgctgatg gaacgattcc agaagcagtg gctgaacaag tcaacacgct attacgtgga
     8041 aacttcaaac cagaattctt gaaccgaatc gacgacacga ttttatttac accattgagc
     8101 ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc aattggcaca acgtctggaa
     8161 catcaagaaa tcctgctgac aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct
     8221 tatgaaccag catatggtgc aagaccattg aaacgattta tcactaagga agtagaaaca
     8281 ccactagcta aagaaatcgt tgcaggacat gttatgccta aatcaaaagt aacgattact
     8341 ttacttgatg gacaacttca tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc
     8401 attgagacta taaaagaaaa aaggggtctg acacaactag tgtcagacct ccttttttca
     8461 aataaacagt gttccagaag tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc
     8521 ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt tataactttc
     8581 aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta aggcagcacc
     8641 tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc cgacaaacag
     8701 tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt cgcataagaa
     8761 ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga ataagaggaa
     8821 caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa tcaatccata
     8881 gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc aaaaagagat
     8941 gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga aaaaccagta
     9001 aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga cttgtggcat
     9061 gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt gagaagtcga
     9121 atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac caaaagcaag
     9181 taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca tctaaaaatc
     9241 tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc aatttgatca
     9301 aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct tgtagtgcag
     9361 cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag ccgatcactg
     9421 ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg atcgtcagcc
     9481 catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca gcaagagcaa
     9541 tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct tgaggaagat
     9601 agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg ttatataaca
     9661 aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca tcatcctttt
     9721 tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc tagtgacttc
     9781 agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc acggcgataa
     9841 ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc tataggttca
     9901 tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc attattccaa
     9961 ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac aaacggaaac
    10021 cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc gatctgctct
    10081 ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat atctccatgt
    10141 acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt tcgagcttct
    10201 tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca gtagaacaga
    10261 acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc atcttcattt
    10321 tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc atggttttgt
    10381 tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa tcgctcaacc
    10441 ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa ggaaagttta
    10501 tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg atacaaataa
    10561 gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag atcttgcatc
    10621 aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc cattttataa
    10681 cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat cagtttattt
    10741 tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga acggataaaa
    10801 gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg gaacacatag
    10861 gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa ttcaagattt
    10921 tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat aacagtatag
    10981 caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta aattactgct
    11041 tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta tggatataaa
    11101 gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc atatcgacca
    11161 tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga cgtctcgcga
    11221 aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa tcacgattaa
    11281 attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata tctttcactt
    11341 gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg
    11401 tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa cattggccaa
    11461 tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata aaatcgacct
    11521 acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc
    11581 tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata ttctcgagca
    11641 gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa aagctttgat
    11701 ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga tcacggacgg
    11761 tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat ttgatgttac
    11821 agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg tcggtgatgt
    11881 cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg atacagtaac
    11941 gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga atccgatggt
    12001 ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg aagctttaga
    12061 aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc aagcattagg
    12121 ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc aagaacgctt
    12181 ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt accatgttaa
    12241 taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg aacctgtaac
    12301 gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa atgattttgt
    12361 cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga tggattattt
    12421 agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg tctttgactt
    12481 tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg aaatgtctgg
    12541 ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa aagtcgatgc
    12601 attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa
    12661 actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag ctattggaca
    12721 aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta
    12781 tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag ggaagaagcg
    12841 gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat
    12901 ggatgagcaa gatagtgtga aataacttat aatcaatcga aaaacctatt aaataaacct
    12961 ttttgagtag atgcaacact gaggattatc tgtataaaag attttaaact gtcatttggt
    13021 tatcacggcg atactaaaat taacaaaatt ggaatatttt tcttgaagat ttttagctgt
    13081 cagtatatga tgaaagcatc tcataggagg tgcttttttc atggttgata attcaagtgg
    13141 aagggttctt aaatctctaa gaaaagagaa aaaactatct caaaaaaagt tagctgattt
    13201 agcaggtatt tcacaaagta ctttagttaa atatgaaaaa gggtcaagaa aaatccctaa
    13261 agacgtagat aatactctaa gcaagattct gaatatagaa actctaataa aagatgagga
    13321 agataagatt gagattctca tcggtaaact aattgcatac agagatatga ataagttact
    13381 taacaaagag ttagctgata atataggaat tagtgaagta ttactcagtt atgtgttaaa
    13441 tagaaagaga aatccgtcaa aagaaatgca aaaaaaaatc gatatttttc tattaagtaa
    13501 tgaaaaagaa attttgaagg agattaatcg tgatagtgag atatttagtt tatcaaaaga
    13561 cgataaaatt gttatgggaa aaagaatacg agaagtccga aagaaccggg aagagacgct
    13621 tgaaaaattt ggtaaaaact ttaccatcta tacaggcaaa aatgttataa gtagatggga
    13681 aaaaggtatt aatattccag atattgaaaa actaatgaat attgcctatc ttggtaaggt
    13741 cactgttcct taccttatgt acggagaaga ttataagaat atacttccaa aagatgaaag
    13801 agttagcgat tttaaaaaga taaattcttt tagtatggga ttaaggatga ggaaaattag
    13861 gaaagattat tatctagaaa gggaggagtt tggaaagctt ttttctcctt caatttcaaa
    13921 atggtccata gataggtatg agaatggtag agatataccc aatactaatc gaattatcca
    13981 atatgcttat ataggaaatc tttctttaga atttttgatt tacgggatat aaaaatagtt
    14041 tacgtcgata cttattaagt tatcgacgtt ttttattata gaaaaaattt ataaaaggag
    14101 gtttcttgat atgataagaa acaaaaaggg tttgttgaaa gttgctttac tggcagcttt
    14161 tttctaagta actttttgac aagctcaatg gcaacacaag cggaagaagt aatacaagaa
    14221 ctaactgtta tgaataacag ctagataaaa tgggctatgt atgttatcca acaatttagt
    14281 aatcactaga agcctagcga aagttcgctt gataagttat agatgagtat tgatttgtag
    14341 tgtttccaaa tcaaaagggt acagagccac atgaaatatg ttgtcctctg cagcgtcagg
    14401 atgggaatcc ataccatgga gattcggaga tctgtgaggg aatacttacg tggttaatat
    14461 gtcagttgtg ttggacgtag taatggtcat agtgggtaga ttgactgaat tcttatatga
    14521 gaaaatgaaa ccaacaagaa gatagggtta tttatacaga attggggatc ttttctggaa
    14581 aagtaatcgg ataatgcgag attcgtacgg tcttttttaa atttctctaa aaaaaacggc
    14641 atcgaatggg ttgggtgatt gataagcaat gaatcataga ctgatataga ataaaaaatg
    14701 attctattta tctttttaca tctatcgttt tttcaagtag ccgcaaatta tatcagcatt
    14761 caacaatttt tgtatacaag ttctagtaaa aggatctgaa gaaaaagttg gttgttgtga
    14821 agataaaaaa agaatcaggt tttactgagt ttttcttcaa aatttgtgtt attaggttag
    14881 gacaaagata agaagaagta tgaagattta aaaaaggaat gaggaaaaga tagtaatttc
    14941 cgattattgg agttttttta tttataaaga agaagcgaat aagttgtgat tcctagattt
    15001 cgcgcgtgat taattaatga gcaaaaaatg cggaaagttt tagtgtttaa tacacgagga
    15061 caaagtttct taggtattga aggcacacgt gtaacggttg atgggtggtg tgattgtttc
    15121 gaggttggaa aacatgttga acttatggca tttactgagc taaaagataa gaacaacgtg
    15181 gaatttttcg agagtgatat tggttgggac gatcatgaag aagtttatgg aaaatttatt
    15241 ttgcggaagg gaaattctta tacgaatggg gaagcataag tagcgatttg ttagagatca
    15301 ttgataggat tgaaattgtc gggaatattt atgagaatct agagttattt gaggtgatag
    15361 aatgagtgat ggttttgaac aagtatcaat aattaaaaat caagttcggg agattcttag
    15421 aaaaaaaggc tatttggtag atagttattt tgaaggagat tacgaaacat gggtaggtgt
    15481 ctatgctcgt ccagagaata aacctaccta tttagatcct acgacgtcag aagatgccta
    15541 tttacaaaat aggtatcgag tagatggttt taagcaagac tttgcggagt ggtttgaatg
    15601 ggaaattgaa aacggagaag taaaagaaga ataagagtac ttgttgtatc aaaataagcc
    15661 agtagaacaa ccaaaccagt agctagtaat gacaataata ctgaggaatc ggatcattat
    15721 ggtatgattg atatttctct tccagaagat cttaagccag atcctgtaac aattggcaat
    15781 agtgtcatga tttttgattc attagatgaa gctatgtcat atggttggaa agaaattaag
    15841 gatgaaggta gtaattggta tggcatgaga tttggtgatg gtgaagttgc caaaaaagct
    15901 ggtattccaa ttgggaaatg gacagttgat ttccacgaag caggttcttt ttttgttctg
    15961 taattatagt gggagggtaa aatcattgat gaaatgtgtt cagataagag cgccagataa
    16021 aaatagaaga gacagttcac gtcgttatca cttttggtaa cggcgttttg ttttattata
    16081 taaaattctc aggaaaagga ggaattcttg tgtatgcaaa aacgaaaatg aattggttga
    16141 aagcactcgc attggtggct ttttctcaat tctgtttctt ggtgggaact gctcaagcac
    16201 atgcagaaga aacgacggct atacaaccta cgatggaagc cattgcccaa gtcgcttctg
    16261 aagtgacaga agtccaacca gcagaagccg gggtcccagc tggtgaagcc actgttgaac
    16321 aggcccctgt gacagaagcg ccaacggtca attccaaaac gactctcgat cagacgattg
    16381 gggaaacaca acacccaggg aatagtcctg gtacttcaca accagttgaa gaaactgcct
    16441 caattacaac ccctgcttca gaaacgtcca ctctagaagg agaaaaagct gccgaagcaa
    16501 cgccttctgt gcagccagag tcttctgaga aacaagcgga agaaaagaaa cgaatagaag
    16561 aaacagtgga acaaccagcg gctactgctg acaaagaagg aaaaaatgag gaaaacgttg
    16621 aaaaaacaat ctcttcaacc gcagctagtc aaaatcaacc aacgaaacca aacaaagaat
    16681 ctacggctag tattgaaaat caaaagaaaa aagagcagaa cattccaaaa acaggtacac
    16741 ctacactgtt agtaacagta ccaaccaatc gttcggttcg tgttcgccgt gcaaccaatg
    16801 cagctgtttt aggtggtcgg aaaattgatt ggggcgaaaa tattggtgtt acttctagtc
    16861 cgaacaatct aaacttcact ttaaattata tggttcgttt tgagattaat ggacaagtgg
    16921 cgttttgtat tgagcctggt gtctcagccg cagaaggtca agtttataca gctactgctt
    16981 tgaaaaacta tttgaaaaat tcaaatgtac ggaaaaaaac aagcttgatc tcttatttag
    17041 gatatgtcac aaataaagac aaatccgatg aacaatacat tgcggcacaa tttatgattt
    17101 gggaagcttt gggcacaaaa atcccaatca aaaatatccg tgttgattat gatcgtcgaa
    17161 aagcagaaat tcaacgtttg attaataact ttggtgaaaa agcaactttt aataatcaaa
    17221 cacatacggt aaaaattggc gaaacgttga aagtaaagaa taaaaatgat tttttgaaat
    17281 atgtgaaaga cattcaaaaa ccaaaaggtg tcaatgtatc tattcacggc aatgaagtat
    17341 ggattacagt cacaaaagag gcaccggata aagcaaaaat tcaattgaat aaaattaaag
    17401 ttcaaggtgc gccattagcc tatcgtcgtc caggagctca aacaattggt gtgttacatc
    17461 caacagaacc taatgcgtct gttattaatt tacaaattct aaaacaagga aacattcgtg
    17521 tgtttaaaga agatgcggaa actggtgtga aagcacaagg tgcagcttct ttagaaaatg
    17581 cggtctatgg tttgtatcaa gcagatggga aaaagattaa agaaattacc ttgaaaaaaa
    17641 ttaacggcaa agtacaggca gaaattaaag atcttgatcc tggcaaatat gttttaaaag
    17701 aaatcaaagc gccaaaaggt tatgtgcttt ctgatgaagc aattgaaatt gttgttgaac
    17761 ctggaaaaac tgtcaatgcg accgcaaaag atcaagtgat caaaggaacc attgatttag
    17821 tgaaagttgc taataaagat ttagtcgatt caacgaatcc agataacaaa ccaaaattag
    17881 ctggcattga aatcactttg acgtcaaaaa caactggcaa agtggtgaaa accgttgtga
    17941 cagacaatga tggttatgct acttttggta aaaataccgt tgtctttgat acgtacattc
    18001 tttctgaaac caaaggaaaa gaagggtata aactatttga accacttgaa gtcacaattt
    18061 ctgaaaatgg gcaaactttc cattatgttt tagaagataa agtaattgaa caacgtctta
    18121 aagtggtgaa agtggatcaa gaaacaggaa aagtgattcc acttgctaat acagaattca
    18181 agatttttga tactttagcc aatcgttatg tcacaatgga gattccaaat gatacagaaa
    18241 caacggatat ctttaaaacc aatgaaaaag gctttttcct aactaacggt acacttactt
    18301 atggaaaaga tcgctatcgt ttggaagaaa tcaaagcccc cgtaaattac gttttgaatc
    18361 aaaacccatt agtgttctcg attaccaatg atacagaacc gatcaaagtg atccattttg
    18421 cgaaccgtca agcacggaaa agtgtaaaat tattcaagta tgaagaatgg aacaagaaga
    18481 aaacgccgct cgcaggtgtg aactttacct tgtataacgg taaaaatcaa gcaattggtg
    18541 aatataccac tgatcaaaac ggggaaattt tagtgaaagg tctagttgcc gatgactatt
    18601 acttcttgga aaatgcccca ttagaaagtt tccaaccaaa tacagataaa caagcattca
    18661 cggttgcatt caaacaaaat gcgccagatg atggggaaac gttagtggtt tctgtacgta
    18721 attacttgat tccaccaaaa ctagaaacac aagcgtcaaa taaagccgat ggtgaaaaag
    18781 tggttgatcc attagaaacg atcgtcatta aagacagtgt ttcttatacc aacttgtttg
    18841 tcggaaaaga atacaccatt tcaggagtat tgatgaacaa ggcaacgaat gcgccaattt
    18901 tacaagatgg caaagaaatc acaagtacct tggcctttat tgcagacaaa gtcaatggca
    18961 caaaagacct tgaatttgtg gttaatgcta gtgttctgaa aggtcaaacg atcgttgttt
    19021 ttgaaaaact tttccgtgat ggcattgaag ttgcagcaca tgcggatatc aacgatgaag
    19081 cccaaagtgt gaaagtgaat aatccaaccg ttcataccac ggctgtcttt gaagacggat
    19141 tgacagtcgc agacccaaca ggattgttta ccttaaacga taccgttgaa attaccgaga
    19201 ttcttccagg aaaagcatac ttggtcaaag ggatcttaat ggataaagca accggtgaac
    19261 ccttgcttgt gcatggaaat acggtagaaa cgagtgttgg ctttattgcc aaagcagaaa
    19321 atgaaacggt gatcgtacca tttacctttg atttgacagg tataaatggt cgtgaaatcg
    19381 tcgtgtttga aagtcttttt agaaatgatg aagaactagc aaaccataaa gacatcaacg
    19441 acaaagggca aacggtacga gtaactaacc caagtgtacg tacaacggct acacataaac
    19501 ccactggctt gaaagaggtt aatccattaa cgaaagtcac cattacagac gctgtgaaat
    19561 accacgattt ggtcgttgga aaaacataca cagtcaacgg tgttctaatg gacaaagtaa
    19621 cggaaaaacc agtgctagta gatggaaaag aagtcacaag taccttaacg tttgtggcag
    19681 aaacaacgga tggtacagtc aatctagact ttattctaga cgcacgtgcg ttgcgaggca
    19741 aagaagtcgt ggtctttgaa gatctgttta gagaaggcgt attattggct tctcacgcag
    19801 atattaccga caatgaccaa accatcaaaa tcagtaatcc aaccattacg acgaaagcga
    19861 cgaataaaaa tggcggaaaa gagatcttgg ccttgccaaa tcaaactgtg cttgaatggg
    19921 tcaaagtaga cggtcttgcg attggtcaaa tctatcgctt aattgttcaa ggctacttaa
    19981 caccagacgg cacaccattt gaaggcactc atgcggaaaa agtctttgcg gccgataaag
    20041 aaatgatgga attcttattt gaattcttag tggacggtcg caatcttgca ggaaaaggct
    20101 tgagttttgc ggaatggctc caagcgaaaa cggaagatga gaaagaaacg tttgaagaag
    20161 tggcgaaaca caatgtagat ctaacaaaca aagatcaaac ggttcaaatt gtggaagcgc
    20221 caaaaccacc gcaaccagtg aaaccagaac aacgggttaa accagctact gttgtaccaa
    20281 tgttgccaaa aacaggtgaa caaacaagta gtatacttat ggttcttgga ttagtagtat
    20341 tagtcattgc tttaggtata acactttata aagtacgtaa agccaaagat tagtcatgag
    20401 gaacaagcgg ttaggatttt taaccgcttg tttgattgta atgtagaaat atttagaatt
    20461 tattttggtt tttaagagga ggaaaaactt tatggcagaa aaagcattgc gtatgaagaa
    20521 agagttttta ggtcaagtat ttttattggg ttcacgggaa cctaatccac caaaggaaat
    20581 taatggtgtg tctgggcgat atgttcaatt acaagcagag tgttcgcgag aagaacagcc
    20641 tgcttttttt gtttatctca cagctgaaga ggataaaaga ttacgattac cagaaaaaga
    20701 ggtgcgtatt tcatttgaag aagcttattt tgtttttcgt aaagaacgag tgaatagtcg
    20761 tgaaacaaat gatatgggac aaacagagcg tcgaacaact ggctatcaag atgttggtgt
    20821 ctttttaact ggtgtaaaaa ttggggaggt tttgtaagat gggattaaaa tttccaaata
    20881 attatgacat tacagaagca ttagatgaaa cttgtttagg agccgtacgc tttggcgcat
    20941 tagaagagtc tgatcgtgta tttgataatg aaacaggcga ttatacggga gaaattcgat
    21001 cacagcgagc acaattagtt tttggaaatc cagatgaacc aaagattgca cgacgagtta
    21061 aatttccagc agatgtagat ttgactggtt ttaaacgacg tgatcctgtg aaattaattg
    21121 atccagaata tcatgtggta tatattccag cacaagatgg caatcgtgaa tattcagatt
    21181 tagtgattta tgcgaaagga ttagagaaag tagaagctcc aacaaataat cctaatatga
    21241 aacaatcaca agaaaaaggt ttagatcaaa aacaaccaag tgttgattct aaggaaaaag
    21301 agaaaaaata aggttttttt gaaagaatag gaaggagtct tcctattctt tttatctaag
    21361 atagaaggaa ggggagattt tgaattcaga taaaaccaat caaatacttt gagagattgt
    21421 gcttgttatc aaaatatcca acaattgatt gagtattttg gagacaatcc aacgattttt
    21481 gtacaaacaa atgcatatat ttgtgcggtt attgggcagt gtatgttttt acgtgatctt
    21541 gttcgttttt cattaccgta ccagtggaaa caagatcatc aggaagtgat ttggattcgt
    21601 atagtacgaa tatgtaatat gtgtgcacac ccttataaaa atcgattaaa tctttttcga
    21661 ttatgttttt ttactaaatg tgatattcct gtattagttg ctttttgtca aaagttttta
    21721 gcacaggaaa agtaaaaaca gaaaggagga atgtgacaat tgaaagctca ttttttcaag
    21781 tatcgtggtc ggcgaattcg ctatttcaat cgttatttat ggttttgcta ttatggtttc
    21841 tttttgtttc catttttgat tctgttaggt tggtttagtt ggaaagtatt ttatccacgt
    21901 ttaagtacgt tttcgaattg gcaaatgtat cttattcctg gaataagtat tgtgttgtat
    21961 ttgttgctgg ttagtgggtt aattttaggg ttgatgcatt ggtcgcgact aaaagaagga
    22021 tattttgcct ctgtttattg gcgtcagtta cttgttcgaa tgttaattga caatcggttt
    22081 gtttatacaa agaaacaagt tagtgagaaa caaacaaggg aaaaaatgag attaccaaaa
    22141 gtttattttt atcaaaagaa acaagaatta gtggtttctt ttcctttaga tggtgggcgt
    22201 tttcacgaac gatttttaaa aatgggtcat ttgttatctg aagtattttt gcgtgattta
    22261 attcgtgaag aacgagaaaa agcccgaatc aattatgtat tcctttcaga ttctggtcgg
    22321 attcctattg atcaatgtat agcagaaaat ggacgtattc atttaatgaa aactttggat
    22381 tgggtatatg atcaatcacc aaatatgttg attgctggtg caatcggtgg tgggaaaacc
    22441 tatttattgt attctttaat tcaagcttgt ttaagtgttg ggacagtaga tatttgcgat
    22501 ggaaaagcgg cagatttagc agcattaggt gacgtcgatg tattcaaagg acatgtgttt
    22561 tacaaaataa atgaagaaat gattatttgt ttacgaaatg cattaaaaga aatgaatcaa
    22621 cggtatgcct atatgaaaac catgaaagat cctaaatatg aaccagggaa aaattatgct
    22681 tattatgggt taccaccgca ttttatcttt tttgatgaat gggcagcttt ttatggttcg
    22741 ttggatatgg aagcgagaaa tagagtcgat aaattgattc agccattagt attaaagggt
    22801 cgtcaagcag ggatgtacat tattttagca atgcaacgac cagatgcaga atattttcca
    22861 agtggtgttc gggataattt aacattccgt atttctgttg gtcgattgtc tcccattggt
    22921 tatttaatga cttttggaga agattttaaa aataagccat tttacaacaa agatcaacct
    22981 ggtcgtggat atgcttttcc aggatttgat gtacctgtag agttttttgc gccatttgtt
    23041 tacaaagatt atcactttat caaagaattt gagaaattta ctgaaatgat tgtctatgat
    23101 tttacagtaa aagaggatag tcaagatcaa acgatgggag atgataatca gttgttacaa
    23161 gaacaactag agcgccaaat gtatttagca aaagatccaa aaggaacaag tttttctgat
    23221 gcgcatattt tgtaatcaac taaggaggtg aagttaaatg gcagagagtt gttattatag
    23281 agtagctaca gcaatacgaa tgattaatcc ttctctttct tccagaacgt tttatgattg
    23341 gttgaatcgt attgaacaag taactgatta ccgctttttg cgcaaagaac gtgtttttac
    23401 aggaaaagtt attaatcaag tattgctaac gaaaaaagat atagaacgat tgactcgatt
    23461 gtatcattat cgagtagatt tagaagagga tttaactttg tcaatttatc gcgttttttc
    23521 tcctgaaaaa tatagtgaaa taacaaaatt agatcattta attttgtagt tattatctat
    23581 cgggagggct ctagacgtag tctggtagcc gcactctgcc tggcggtgcg cattaaggct
    23641 gcgaagcatg ggcctataat gcgcactcgc tagcagcgtg tgcagctcac aaagaggtcg
    23701 tgcgcttgcg cacggcctct ttgtgtaaag ccccccgata tataatatgg ggggcagaaa
    23761 taacataaca catagaaaaa agttttataa acccttttgt atcaagtgat tatctaaggt
    23821 cagcagttgt actacttttc ccaaaaaacg agggggacta gttgatatat cggtgtttct
    23881 aaagggttaa aaaaaatcga ttttgttatt ttataaaagg agcgagaaaa tgacaccgat
    23941 tgagctaaaa agctacagaa agaagttaag tgcaattcat aatataagtg gagataaaaa
    24001 gccgctatca caagcaagat ttgcgcaatt gatgggctgc tcaaaagatt acattagaaa
    24061 gatggaacaa gggaaaagaa aaatcccaga ggattttgaa caacgaatca aggaacgctt
    24121 tcgattgtat atgaaaagcg gaatagatct tcgagcaatg attgattatt tgcgtctttc
    24181 cttttttggg acagactaca aaacagtgat tcaacgtgta ttacgaatgg atctgacaga
    24241 atttgaaaca cgtccaacgg gcttgtatct ttttgactgt ctttctgttc gtgggaatat
    24301 ctgggtgttc tggcattcaa aacgaactgt aacagaaaat gtacttgtgc aattttcagg
    24361 acaaggttgc cgggaatatg aggaagtcat gcgtgaacaa caaacagatt ggcgaacatg
    24421 gctatattcc atttggaaaa atcaaggaaa gttggaagct ggttatacac gtgtgcaatg
    24481 tagccgattc gatattgcgt tagatgaact ttggggattg gaaacggaag aacatttcga
    24541 tctctttgaa ttactagaaa aacaaaaagc aggtttatta gaaatggact ttaagagatt
    24601 ccagaatatt ggcggttgtt ttttagatga tggctatttt cgaagtgaag gattatcttt
    24661 gtattttgga tcgcgaaaga gtcctctctt ttttaatttt tatgagaaac gttttgaaat
    24721 tgcgaaccga gaaaaaattt cagaattaga agcattaacg aaatatggta tttataatcg
    24781 atatgagtta cggttagcca atgagaaagc aacacaagca gtagaagcat ttattttagg
    24841 tgattcaccg aaacgtttag gagagattgg cgttggttta attaatgcaa aacttattgt
    24901 ttgggataga ctggaaagtg atgcacggat tgttgataaa cgatgggcaa aattatttcg
    24961 ttcgatggaa gaattggatt ttacagtaaa gccagaaaaa ttctcattag atcgttcaga
    25021 acgctggttt ttgaaacaag tagctccgac attaaagttg ttacaagaag cagatcgtat
    25081 tttaggaaga gactttgtac aagaagggat tgataatgcc gaactaaatg acgagcaaca
    25141 aaactatcta gaatggatgg gaacgtcagg agggaaaatt gcatgaatgc atatacgaca
    25201 acagaatggc tgcgcctgtt ggatttaaag gctgcaatcg aagcattaaa tgaaaagatg
    25261 gttgatttgt cttattttcg ttttcgagtt ccttatattg aacaagcggt aaaagctgga
    25321 cgttatcagg aaaaagagaa ctggcaagaa attgcacgct tattagaagt tcgtaaggga
    25381 tacgaacaag aactcgaaga gttggaattt agccgaagga aaggaaggct agaattcatt
    25441 cgcttttatc gattttcatt gccaatacct gcgattttag cggttaaaaa aggctgcgat
    25501 aaaatgaaga tatatgaaaa ctgtgtggca gcacttagca gcgaaaaacc tcttatggaa
    25561 gaaatttcat tggtgactgt cacttgggaa atgagtcgcc agccaacaga agaacagaca
    25621 tatctttctt tagaagagat tgagatagaa ttagaggaaa ttggtcgcta tgccacttgt
    25681 tctacctatt gtggatcggt cattagtatt gctggtgtga ttgtctaaat gaaaaaaatt
    25741 atgatttatg ttggtgcgta ttggtcacgt gatccaacag tattagaaaa tccagaggcg
    25801 gtacactatt gcttgcgcca attattctat ctgtataaag aacgattaga atcgttgatt
    25861 cgtcagttac catatactga cagacgttta gatgaacttc ttctaaggta tccagcaatg
    25921 tataaacgac gaaaaaatag attgttacca gaagagtatc ccattgaaaa acatgaactt
    25981 gaagggcgat ttgtggctta tttctatgat gatgttcgta tgcgcttagt agaacaacga
    26041 atggaaatag aaaatgatcg ctactatttt atcaattatt gtaagaagcg taagtatcaa
    26101 gtaactgatg atttttatga ttgtatttta caagatgggg aagtgattct atccaaaatt
    26161 gctccaagtt ttcgtgaact tgtgccgatt gattttttga aaaaatgtca tttgcggatt
    26221 cttccgtaaa aaagagctat aaaaggaaag aaaagtagaa gccaaatcgg tttctgcttt
    26281 tttatttttt ttaggaagga gaagatggta tgtcactgga atttttagga gagccaattg
    26341 aaaaggaaga gttcgtttcg cattatatgg atttgtttat tcaggtgata aaaaactttg
    26401 tgcgattgat gaattttatc cacgcgaaca agactatcta gccgctgaac gaataaaaat
    26461 cagtttatta tgggaaaaat tcacagatga aaataaccga ccgccagatt atcgctattt
    26521 atcagattgt ttaacgaata atgtgctttc taaagaatac ctatttcaca aacgtgagaa
    26581 aaatcaaatg tcggttgaac atttttgtga ttgttatatt gcattattgc gtagtcaaca
    26641 gttattaaca gtagaacgat atggtgcaga gtttggttat gtcttgcaag ctgagcgtca
    26701 tagagctaca agatgctatg aaaaagaaga tcaagtgatt gatggctatg aagaattatg
    26761 tattcgaaac aatcgctatt tgcaacaacg tttatatgaa caattgatga tggatttttc
    26821 aaatctctat agagggtatg aatgttcgat gattcaagaa aggtagaaga aaagggggaa
    26881 tttttatgtg gcgattatta tttttggtgg tatgtggtat ggcattgttc tatgtgtatc
    26941 gtgtctatct ctatccacga atggaacgat ggacaagttg gtatagagat cctttaaaac
    27001 aacgaaaagg attgaagcgg tatcgttatg aattacttgt tgaattttcg gatgggcgac
    27061 aagcacagta ttattttttt gcaaactatc caaaatacaa caccagtgaa ctattgattc
    27121 aattattcaa aaataatcca tatgttgggt tagaagaaaa cggacgggag ttcttttatc
    27181 gttcagataa tattagtgcg gcgtctatga acaaaacacg aattgcataa aaaattggag
    27241 gaataatcat gaaaaaaaca acattagcag tcgtaacaac actaggaatt tcattatttt
    27301 cgtttcaatt agggagttca ttggtttatg cagatgaaac tgtagaaaca cctaagtcag
    27361 aaattgtaga aacattgcca ggggaaggat taccagatgt tgagacagag ctaaatggag
    27421 gaacaacgga aaaaccagca gaacctgaac aattgcagtc agagaaacct gaaaaagagc
    27481 cagtgacacc agaaccagag tttcctgaat caactttacc gactgattcg atcgaagaag
    27541 aaggtgtgca tagttcaaca acaacggatt caagtatagt cacaactccg gaacaatcag
    27601 gaacaaccag ttcttctgaa aagaatactg attcgtcaag tacggaacag ccaacgatgt
    27661 cgtctaccag tgaagaacaa aaagaagaac cgaaacaacc tgttgaaaaa tcagaagcaa
    27721 ttccaaaaga aaataaacct attgttccag caaaagaagt cacagtatcg gtaaccccaa
    27781 gcggtgaaat tacgacgaat acttctcaag gaatgagtgt accgattgtc actagtaatg
    27841 tagaggaatt gacccatatt ccaacaccaa ccaccccatt aaaagtagca ggtgatcaaa
    27901 caattgttgg tgtaaaagat ggtattccat taatccaaga tagtgaaggg aatttggtaa
    27961 aggatttgtc gattcctgta aagaaacttc caagtggcaa tattgaaatc aaaacagcag
    28021 atggaaaaac aaaagtctta ccaaagactg gtgaagaaat acatgttgca ctttctgttc
    28081 taggaggagt gttgacaagt attgctggat ttgttggata taaaagaaag agaaaaggaa
    28141 tagaaaatga gggtaattga atggactatt tttcagaaaa acaagcttat cgttgtcaat
    28201 cgtacgtact agtaaaaaca tactagaagg agtagcttat gaaaaaacga caactacggc
    28261 ggaaagaaaa agtttataca gagaaagtac cgaagcaaaa gaaaatccat tcaaaaaaag
    28321 cagtgaaatg gatttggtgt ttggttttat ttctttgttt ttcagggggc ttggcatttt
    28381 ttcaatccat gaaaacgaag gttcaagtgt cgtttttgaa agatgaaatc caacaattac
    28441 aaaagaaaga agtacaaagt cggtcagaat taacgtacac acctgaaatt gaatccttta
    28501 tgaatcgttt tgttgctttg tatatgaata cattcgataa tcatgaacaa gaacaacaac
    28561 gtcaagaaac attaaaaaaa gagttttatg cacaaaactt tattcaagaa ccaggaagtg
    28621 tttcaggtgt tcgaaaatta actgctgctt cgtttattgg attaaaagaa attgaaacca
    28681 ttccaactgc tagttataaa gtaacttatg atattcgctc gatgaatcca gagagtaaga
    28741 aagaaacaag tgtgacaatc acacaagtac tcaatcttcc cttttctatt tcaaacaagg
    28801 gctgtcgtat tattgcttat ccttatttta cggcactacc aaagaataca gaaaaacaac
    28861 gaatgattac ttatgatgac acattatacg aaacggttga tgcgtcggaa gcgaaagatg
    28921 ttgaaggatt tgtggaagat ttcttagata agtatgcgaa aaacaatgca aaagatatgt
    28981 catacatgat gaaagaacct gaaggactca atcaaacagc taaagttgat caagtagaga
    29041 tgaaagtatt gcgttataaa gaaacgaaaa aaccttttat tgtaaaagca acggtcgtct
    29101 ttcgagatgg tgatacgtca cttttacaaa aagaacaaat gacgattgtc attacgaaaa
    29161 aaacaaatca gtattatgtt gaaaaattaa cacataattg gttggatagg aaaggagaag
    29221 aacggaaatg acactgaagg gaattgtaga ttttatatta acgcaaggtg gctatttttt
    29281 aataatggtg gttgtttatc tgggattgcg aaattttatg aaatcaaaag taggaaatgc
    29341 attaatatct atcgcagcag gtgccgcagt ttatttcttc ttaaatgatc caacacgtgt
    29401 atttgatgcg tttggtaaaa tcattgaaaa attatttatg gattaggagg atagtgaagt
    29461 ggattgggaa ccttatgatt attcgattgt ttatgcggaa ccttatcgcg tacaacagat
    29521 tacaagcaat tggcgtcttc catttccagt tgcattagtc aatgcagcaa cttttttgtt
    29581 ggtcgtaatc tttgaatatt tatttttacg ttcatttatt ggttgggtca tttctttttt
    29641 tccaccagca ggagttgttt tttatatagg actcccctat ttatgtgtgt tagcactggc
    29701 aaaactaaaa cctgatggaa aaaaagtgca tttttattta tgggattggt tttgttatat
    29761 gattcgtatg atgaatggtg attgtgtgta ttataactgg gacaaggtat caaacaatgc
    29821 attggaagaa ttactttttg tgaataagta tgtttattta gaggattaga aaaagggaca
    29881 atcaactggt tgttcctttt ttattgggag gtgaaattca ttggtgcttg taaaagaacc
    29941 agtaaaagca ttcaagaaaa attgtttgtt aaatggagag gattgtatct gggcctacta
    30001 tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg aaataaaaga
    30061 aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt tggtgatgta
    30121 tccacgaaca atgaatttac ggacgaagtt tgacacatta ggaaagggat tttctcccga
    30181 tagtcaagca attggtgcgt attatgcaaa agaaacagtg acattgttgg aacgagaagg
    30241 tcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag caacggtgga
    30301 gagttttcat gatgtaataa atctaatctg gttagattta gcaggaagtt tagcacgctt
    30361 atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc cttatgtaaa
    30421 agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa aagagctgtt
    30481 gtatctcaat cgtctgcaat atattcgagg aatggctcac aaagtagaag aacaaaataa
    30541 ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg aacataaagg
    30601 ccttcttcaa ttagaagatg agttagggag tagttattta gcattgttgc ctattgggga
    30661 aacacctgaa aatatgagaa attgtgatct atttcaattt gctcaggatc tttctttccc
    30721 agcagaattg cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag gtattggttt
    30781 aaaactgaat tggctatcgg aacggttcaa aacagagcat aaggaattgg ttcaaaatga
    30841 tgaagaacct agtgaacgct tattaactgt tcgaggatta gtaaaacatt taaagcaacg
    30901 agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat acggtcaaac
    30961 ggttgaagaa gttcgtcaaa atagtgcaac ggttattcaa caacttcata cgattatcca
    31021 tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat tagggcaacc
    31081 attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg gcgaatggtt
    31141 gttttcagca gtatcagaat taggtatgga atccggctgg tatattggtc ggcaagatac
    31201 attaggggat ggtggttctc gaggagaaaa aacaccatta gaagaattaa tttatgcgtc
    31261 aaatcgtttt gtctttttga atcttctagc cattgcggaa ggaatcaaag gtgcgttgtt
    31321 tgatgcgcct catgtagcag taactggaaa aaccggtaaa gggaagacct ttttagttgg
    31381 cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtgg atcccaaagg
    31441 agaaaagcgc aaatggttta cgaaactcat tcaagacccg tattatcaga aacattaccc
    31501 tttgtttgta gaacatttgc aaaaaatgag ttatgtcacg ttagatgcta caaatccccg
    31561 taattatggt gtattagatc caattgtttg tttatcccga gtagatgcga aacaagtagc
    31621 ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa aaggggcggt
    31681 attaaaggga atccaagaag ttttagttca aaaagaagcc ggacaacaag ttggcttgat
    31741 gcatgtggtc gactatttag aacaaatgga acaaaaagag gttcgagaat atggtgagtt
    31801 tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg gagaaaaccc
    31861 gggattagac tttaatgcca aaacaacgat tttagagatc caagatttga aattaccaaa
    31921 tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt gtttgatgat
    31981 tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga aaacagcgat
    32041 ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta ttattgcagc
    32101 aatggcacgt gtggggcgtt cccagatgaa tcaattagtg ttagatacac aatttattgg
    32161 tgacttaggt tcagataaag aaaaagggaa ttttggtgtc gtctttgctt ttgatgaaga
    32221 cagtgagcga gaaaagattt tgaatcattt acgtttggaa gtaaatgaag cgaacttagc
    32281 tgaaatgagc cacatgatta aaggacagtg ttggatgtta gatccctatg gacgtgtagg
    32341 aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa caacggaaaa
    32401 aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga aatgaggtga
    32461 aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt ttttaacgat
    32521 tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc aggtggatca
    32581 atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt tatctgatag
    32641 tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc taaaaatgat
    32701 gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt ttgtcattga
    32761 gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca ctttttcgga
    32821 tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct tgtatgtgtt
    32881 atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg tgtttattcg
    32941 atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta acgtacctag
    33001 taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag aaggctttat
    33061 tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg gtgttttagt
    33121 gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata aagcggctgt
    33181 tgttgatccg tatatattgt taaattatgg gacaacaaat ttagaagaat tgtatcaagc
    33241 agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat tatctgatat
    33301 tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg cttatcgtgt
    33361 ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg tgccattgct
    33421 taatctaagt attggattac cacttgtagt gattggcatg attcgtttta tctttcaaat
    33481 gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt ttgttccagt
    33541 atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt ttcaaaaatc
    33601 tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag acgatttaat
    33661 tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac tcctatcaat
    33721 tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag ctgcagatca
    33781 aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga aacaactgcc
    33841 tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat tagcagggaa
    33901 tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc aaaagtttca
    33961 acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtggttaatc cctttgaact
    34021 aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt atcatggggt
    34081 agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc tttcaaatgg
    34141 tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac aatcattggc
    34201 acaattgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata aaggtatcgc
    34261 ttttgttggt caatcgattc cgttgccttc tggaaaagta cccgtgccac aattaacagc
    34321 tgctggttct ctttcacaac agattcgcca agtgaaaaat caggcgaaaa atgggagtta
    34381 tccagcaatg cagcagttat tgaaccaata ctctgttcct aattcgtcgg tagccactct
    34441 atcaggaaat cgttctgtgc aacaaccggg gcaacgttcg tctgaaagta aaccgtcgat
    34501 ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagccaa cagagcaatc
    34561 ggaaaaaata gaaaaggatc ctaaaacact gccgattttg gacgcaaaac caggaactga
    34621 ttttacttta ccaaaaggac gaacagtcca aaaaccatta tcgaagaaag gatgaggaag
    34681 aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt tttctattgc
    34741 cattcttttt ttcaggagaa ctttctgagc atggaggaga agctacaggt ctagctgtcg
    34801 cttttaatgg ggaatatacc gaaggattac cgattttttc agagattaaa ggacgtggtc
    34861 caatcacaga tgaacatgcg caagcagcag ttggtgcagc tgtaaagtat cagttattac
    34921 cgagtgtgat tctttctcaa ttaggctatg aatcagctta tggacagtca caatctggaa
    34981 gaaacgatac gaactttttt gggattacct ggtttcctgg ttgtccctac ccacaaggtt
    35041 ctgcacgtgg aattggtggt tctgaaggtg gtttctatat gaagtttccg aacttaaaag
    35101 cttgttatga ctattatggc tttatggtgg cgagccaaag caactttaat caatgtgtgg
    35161 gcaataaaga tcctggaagt tgtttattga ttctaggtcg tggaggctat gcggcagcag
    35221 gtattaccga agggagtccg tattatcgag gagcaatgag tatcataacg tcctataact
    35281 taacggaata tgatactttt gcgatcaagc attggaatag cctaccaaca aaaaaaactt
    35341 cgcttattgc aggaaaaagt aaaggcaatg cgaaggtttt ggatcggatc attggacgat
    35401 ccgtaaataa tggggaatgt tatggtggta cggcatggta tgtatctcaa ttaggtggcc
    35461 cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat tataactgga
    35521 caacctatgg ttggcaagta atcatgaacc caaaaccaag tgatttacaa gcaggagatg
    35581 tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac accggaatta
    35641 taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat gccgagcaag
    35701 gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt agtctggttc
    35761 gtaaggtgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa ttttagtatt
    35821 tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg aaaaacaaaa
    35881 aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa gtggaactct
    35941 tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag cgcaacaaat
    36001 gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat ggagtaattt
    36061 cacagatgtt tataaacgca atcaagctgt gagggactat atgacagatc gttgtattaa
    36121 agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga aactttacca
    36181 ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag aaacaatgaa
    36241 taaggttact caatttgtat tacttcaatt gaaagtagat caagaagctc aaaagattga
    36301 tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaaaatgaa cactcatatt
    36361 tattatacac cgaagaaata gaaaaaaaca gactgttttt ttaagaaaaa ctatgtcatt
    36421 ctttacttga aaaaagttgt tataggacag aaaggataaa acaaaaacgg aaaaaatatg
    36481 tcttatgaat aactataaga actattctat tttttaggag atggatacga gatgaaaaaa
    36541 ctaattggct atagtgtgat gtgtattgga tttgttggtg tgggattgtt tgtatcgact
    36601 gaatctgctt ctgcggctga atggtataat cctatctggt ataatccgaa cattgaatgt
    36661 caaaaaggct atccgtatgg accgacaaat ctgaaaaatg gaaaatgtag agcaaattgg
    36721 aatggcgttg tagatagtgt aattactggt tcaatggact ctgctctaca tggctttacg
    36781 ggtcactaga aggaggaaga aaatgtctga agagaaaaaa gaaatcgttg aatcattagt
    36841 agctttaaaa gattctcttt ctaagattga atatgttgat aggcaagaaa ttcaaaaact
    36901 aattgatgat actattattg aaatccaaga tgcgcgttgt gaaggtataa aaatttctgt
    36961 agctttatct aaagtaatag aaaaaatgaa ccgttcatta gcgtttaatg gattgaagct
    37021 agatagacaa actagtctga tatgggatca tttaaaagac ttgtatgaca agtctaaaag
    37081 aagtgaacgg actgctgtat caatcttaaa aggcctttgg ggaatgaatt cataaacaag
    37141 atatttaaaa agtaacttat tttttatgga ggagaaatta tgaaaagaat aattaaaata
    37201 tttggtgttt taggtgtttt cttattaggt ttaagtactt ttgttagtac tggaacaagt
    37261 gtgtatgcca cagaaaaagt gaatacagtt gatttagaaa taaagaataa attagtagat
    37321 gaattaactt tttattttga tacagttggt tttttagacg aacatggtgt ataccatata
    37381 aaggattttg gtgctattcg tgaacgaatc agtcaagata acgaacaagg aagagcagca
    37441 agagtctttt atgaacatat tattgttcct agaatggaga aagttgattg gaaacgatat
    37501 gctggatgtg tagccattaa ttctcttcct ggaggaggag tggcttgggc tataggtacg
    37561 cagggagatt ttattgaagc attaaaagct tttaattttg aaaaagctgc ggatttatta
    37621 ttaaaaattg cagaaaaaca attaggaaaa gctgcagcta aagagttagc taaggtaaat
    37681 attgtagcag gaattgcagc agctgctgct agctgtgcat tagagtaaaa gtttatgaaa
    37741 agtttagaac aagagcatat ggcttttaaa caagcaatgt ttaaagaaaa tatctattta
    37801 aaccataact atattcgtgt ttctaaggca tgttcaccag tattgaatat gcttggaggt
    37861 ggaaatggat tatatcattt actgtttgtc gatgtttgtt
//
LOCUS       DACDIP010000020        35003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDIP010000020 DACDIP010000000
VERSION     DACDIP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.020"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..1429)
                     /locus_tag="IV257_001203"
     CDS             complement(53..1429)
                     /locus_tag="IV257_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8814348.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(1466..2332)
                     /locus_tag="IV257_001204"
     CDS             complement(1466..2332)
                     /locus_tag="IV257_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8814349.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(2421..3446)
                     /locus_tag="IV257_001205"
     CDS             complement(2421..3446)
                     /locus_tag="IV257_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8814350.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(3464..4138)
                     /locus_tag="IV257_001206"
     CDS             complement(3464..4138)
                     /locus_tag="IV257_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8814351.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(4322..5215)
                     /locus_tag="IV257_001207"
     CDS             complement(4322..5215)
                     /locus_tag="IV257_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8814352.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(5289..6986)
                     /locus_tag="IV257_001208"
     CDS             complement(5289..6986)
                     /locus_tag="IV257_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8814353.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(6979..8292)
                     /locus_tag="IV257_001209"
     CDS             complement(6979..8292)
                     /locus_tag="IV257_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8814354.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(8292..11810)
                     /locus_tag="IV257_001210"
     CDS             complement(8292..11810)
                     /locus_tag="IV257_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8814355.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(12103..12561)
                     /locus_tag="IV257_001211"
     CDS             complement(12103..12561)
                     /locus_tag="IV257_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8814356.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(12858..13376)
                     /locus_tag="IV257_001212"
     CDS             complement(12858..13376)
                     /locus_tag="IV257_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8814357.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(13378..13994)
                     /locus_tag="IV257_001213"
                     /pseudo
     CDS             complement(13378..13994)
                     /locus_tag="IV257_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(13991..14845)
                     /locus_tag="IV257_001214"
     CDS             complement(13991..14845)
                     /locus_tag="IV257_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8814358.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(14842..15753)
                     /locus_tag="IV257_001215"
     CDS             complement(14842..15753)
                     /locus_tag="IV257_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8814359.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(15794..17128)
                     /locus_tag="IV257_001216"
     CDS             complement(15794..17128)
                     /locus_tag="IV257_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8814360.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            17336..18166
                     /locus_tag="IV257_001217"
     CDS             17336..18166
                     /locus_tag="IV257_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8814361.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(18410..18659)
                     /locus_tag="IV257_001218"
                     /pseudo
     CDS             complement(18410..18659)
                     /locus_tag="IV257_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18850..19044)
                     /locus_tag="IV257_001219"
     CDS             complement(18850..19044)
                     /locus_tag="IV257_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814362.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(19549..19734)
                     /locus_tag="IV257_001220"
     CDS             complement(19549..19734)
                     /locus_tag="IV257_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814363.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(20584..20925)
                     /locus_tag="IV257_001221"
     CDS             complement(20584..20925)
                     /locus_tag="IV257_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8814364.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(20925..21155)
                     /locus_tag="IV257_001222"
     CDS             complement(20925..21155)
                     /locus_tag="IV257_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8814365.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            21442..21780
                     /locus_tag="IV257_001223"
     CDS             21442..21780
                     /locus_tag="IV257_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814366.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(21985..22224)
                     /locus_tag="IV257_001224"
     CDS             complement(21985..22224)
                     /locus_tag="IV257_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP8814367.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(22200..22547)
                     /locus_tag="IV257_001225"
     CDS             complement(22200..22547)
                     /locus_tag="IV257_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8814368.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(23279..23860)
                     /locus_tag="IV257_001226"
     CDS             complement(23279..23860)
                     /locus_tag="IV257_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8814369.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(24043..24669)
                     /locus_tag="IV257_001227"
     CDS             complement(24043..24669)
                     /locus_tag="IV257_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8814370.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(24691..26022)
                     /locus_tag="IV257_001228"
     CDS             complement(24691..26022)
                     /locus_tag="IV257_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8814371.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(26177..26578)
                     /locus_tag="IV257_001229"
     CDS             complement(26177..26578)
                     /locus_tag="IV257_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8814372.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(26664..26870)
                     /locus_tag="IV257_001230"
     CDS             complement(26664..26870)
                     /locus_tag="IV257_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814373.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(26965..28467)
                     /locus_tag="IV257_001231"
     CDS             complement(26965..28467)
                     /locus_tag="IV257_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8814374.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(28485..28940)
                     /gene="argR"
                     /locus_tag="IV257_001232"
     CDS             complement(28485..28940)
                     /gene="argR"
                     /locus_tag="IV257_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8814375.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(29038..29655)
                     /locus_tag="IV257_001233"
     CDS             complement(29038..29655)
                     /locus_tag="IV257_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814376.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            29876..31249
                     /locus_tag="IV257_001234"
     CDS             29876..31249
                     /locus_tag="IV257_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8814377.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            31324..32436
                     /locus_tag="IV257_001235"
     CDS             31324..32436
                     /locus_tag="IV257_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8814378.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(32508..34145)
                     /locus_tag="IV257_001236"
     CDS             complement(32508..34145)
                     /locus_tag="IV257_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8814379.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(34313..34921)
                     /locus_tag="IV257_001237"
     CDS             complement(34313..34921)
                     /locus_tag="IV257_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8814380.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         9982 a         7456 c         6026 g        11539 t
ORIGIN      
        1 caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga gtctatttct
       61 cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca ataaccatta
      121 cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca atacaagtca
      181 agatggagac gattggcaat agcggaacga atggcacttt gaattctcca gcttttggtt
      241 tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa taggcgagcg
      301 ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca aagaatacag
      361 ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga tcgatttctg
      421 tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat
      481 acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc
      541 ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg actcctagtt
      601 cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa
      661 tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg acttcctcgg
      721 ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct gcaccatcta
      781 gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta acgaaaaata
      841 ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg gcattattca
      901 aacgtaatgc tttctttgct tcaagcgaca cgatgtaagt gacgaatatc actacgagca
      961 ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt aatgcttgtg
     1021 gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat ccggaagcaa
     1081 cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc caggcagcaa
     1141 attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc actcgcgaag
     1201 caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa
     1261 gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca aatattcccg
     1321 taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt tcttttttca
     1381 tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt gaagttcctc
     1441 cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa aggataccac
     1501 ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc gtttttcttt
     1561 cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt gaaagtgaga
     1621 agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt cctaagcccc
     1681 ataataatcc acagatcatg tttttccaca caaactgatc gactttaact ttacggaaag
     1741 caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt gacatcgcat
     1801 ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta gagaagatca
     1861 atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt tttccttcgt
     1921 catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca ataataccgt
     1981 atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca atcgtattca
     2041 aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg tacttcattc
     2101 catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat ccgatcaaga
     2161 aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa aacagaaatg
     2221 caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta ccacttacta
     2281 aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc ataaccaatc
     2341 tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca caagcacttt
     2401 ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat tgggaaaaat
     2461 aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat acctaatttt
     2521 tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt gctaagtcga
     2581 ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc tgggataccg
     2641 atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat gctcacaatg
     2701 atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt gaagataaaa
     2761 gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc gtagcgaata
     2821 tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat tccttttagt
     2881 ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata atacaaaact
     2941 tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata cttttgatcg
     3001 tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg cactttgatt
     3061 tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat tgctgattgc
     3121 attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag tttttcttct
     3181 tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat gtcggctaat
     3241 tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat cagaaagacg
     3301 ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc
     3361 cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa tgtccactga
     3421 tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga acgatatatc
     3481 cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt tttcggcgta
     3541 accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca tcccacagtg
     3601 ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa ataaataata
     3661 gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata cttccgttgt
     3721 caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct gtgtagttgt
     3781 aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg aaatgttttg
     3841 tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc gtattcctgc
     3901 tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac caataatagc
     3961 catccaaaac gggaagatta atatctagta atacaagctg tggttcttct ctttcaaagt
     4021 ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat ttctcaagtt
     4081 cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt tttgccataa
     4141 aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg tgaaacaagt
     4201 tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa tatgcgattt
     4261 aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa taattggtct
     4321 attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa caccaataac
     4381 cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga taaagatggt
     4441 atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact cgtcttcctg
     4501 tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt gtgactagat
     4561 cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca attttctcaa
     4621 cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca tcttgctcca
     4681 tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg gtaatcacgg
     4741 aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc gctcctctat
     4801 ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt ttcggaatat
     4861 agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg acatttctaa
     4921 ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat gtatgagcaa
     4981 gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct tctttatcgc
     5041 gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg ctgagagcat
     5101 ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact gccataaata
     5161 attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt ttcattttct
     5221 tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct gcagcttatt
     5281 tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta cgctccaatc
     5341 atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt agcaggaagt
     5401 gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc tacgatctga
     5461 atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc ctcacctgtt
     5521 ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc ataatctaac
     5581 ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg tccgcttgtt
     5641 gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc ccctttataa
     5701 aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc tttaagcaat
     5761 tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac tttctctggc
     5821 ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat aaccgctgtg
     5881 ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata cgtgatcgtt
     5941 aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc tactccattt
     6001 tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat ataatcttgt
     6061 ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata ataagttaga
     6121 ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc gttttcaagg
     6181 acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt ttttcctctt
     6241 tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat cgttacttgt
     6301 ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc gtttttcgga
     6361 tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca cacgccatcc
     6421 accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat tctggaaata
     6481 tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga aggtgcttct
     6541 tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt ttgtttgtca
     6601 atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg aatccattca
     6661 atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt atctcgaatc
     6721 aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg aatattttct
     6781 tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac agatgagaaa
     6841 ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc ttcggctttg
     6901 actagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag tacactcatt
     6961 cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta ttaataaaac
     7021 agcacctaac aataaaagaa ttcctagtaa agaaatcaac gttcttgctt cattcgtttg
     7081 cgggaatacg gaccattttc ctgattgacc tggattgctt ggaccactcg gctctttcgg
     7141 tttgtccgtt gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg attcataggc
     7201 agagactgga actttcccgg attctaattc agccatctcc tgtccgttaa ctactacata
     7261 ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg atgacctcga ttctcttact
     7321 tgcttcatcc atctcgtaac cttccacacc ttttaattct tcaaaataat aggtgccaga
     7381 aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta tccgaaatga atttgcttac
     7441 atttttgtca ttcaaaggat cattgtctgc tggtttgatc cattgatttt tcaagtcatt
     7501 agcaggagcc atatctaaat aatatttccc cccttctatc atctgatata gagcaaaaac
     7561 agcaccttct aatggttttc ctttttcagt tgttcctctt tgacgtccat acttgaagaa
     7621 gtatggatct cttagataac cgacattttt aggatagata tgaatctctt gcagttcctc
     7681 ggttgattct gtcgggtgat aaattggcaa aatagcagca attgggatag ctgtctgatc
     7741 gatatcaaca tccaatccaa cttctggatc aatcgattct tcgaaaatca aataaactgc
     7801 atcccgatct tggtttttct taggtgcttt gatacgtgca atcccgtctc ctaatccgcc
     7861 aaccgtatct ttcccagttg ttaccgaagt ccctatttgg acaagccttt cttctgtgat
     7921 tagctgacgg atattgtttc tgttcgtttc agcgatttgc tggacgaatt ctttaggagt
     7981 catgttttca gcagcttttt tttcatgata ataatcggtc atgtcgtaaa tggcaaatgt
     8041 cgctccattt agtggcaccg tctgtgaaat gatatctgct gtttcccctg cttcctcaga
     8101 gatagccaag ccatcatttt catggtattc aaattgatcg ttataatcga tatctcgaat
     8161 catccgttta tgcaacacga attccacgac atcttcccct gattcttctg ccgacatcgc
     8221 atgtggaaga aggactgctg caagcggtag taaaacaagt aattgaagca tcaaacacaa
     8281 cagctttttc attaccttgc tccctccttt ttagattgtc tgacaaacaa accataggct
     8341 gctcctgcaa gcgccatgaa gaatgcagca agcaggatat agaataatcg tccggatcct
     8401 cctgtagcag gcaacggatt ttcaggatcg ttcccgacac taagctgtaa tctgattcga
     8461 tttgtctcca aaagttcata ttctaactct tgctgtagat caggcgtctc tgcttcttcg
     8521 tttggataat acgaaatctc aacttttagt gtacccgctt cttttcctgg aactacccga
     8581 tcattttcta tgtcaggttc ttctcgataa ctaatttcta aaataaagta acctggaagt
     8641 tttctatagc tgtcaggtga cttcacttcc ttgatccgat agaccccttc caacaagtct
     8701 gaagcattga atagggaaga atcgtttgga tctggtgcca agcctccctc gatgatttca
     8761 tactctcctg cactattttg tttctcaatt gaaaattctg cgccttcaag acgtcggcta
     8821 ttcatactgt caattttcaa aagttccaaa tcgattggcg ctagttcatc ttctaaggtt
     8881 acttgtagta caccatcgat gatttcatag ccttccggca agggagcagt ttcgtcttcg
     8941 atgaccgtat cttcaatgat aaacttacct tcttcattca gtgcaaactg gaacacttga
     9001 tcagatagcc gatacccatt tggtgcgaca gattccgaga cctcatattc accaatagac
     9061 agatgcagag gttggtcgta atttaaatcg acatactttt ctactcctac ggcacgagaa
     9121 tagaagtatc cgatcccagg caaacgtttc gattcttctg gttctaacgg attaccagtg
     9181 tatgctcgat acagcgggaa ggaagaatcc cccactttcc tgatatcgaa acctgcacca
     9241 tctaatgtat ggccttcatg gtctaatttt ctaactcgga tctcccgata atcaaacgta
     9301 ttgtaagcag tgaatgctac ccataaacga tcctcttcat ctgttgtata cgtgagatat
     9361 tgccaatttt cttcacctgt ttgcttgagt tggatagtcc ctatagcatt atcgtaaagt
     9421 cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt ctggctctca
     9481 atgattttgt attctgcata gttggtagtt tcttcactat ccttataaac aaatgaatct
     9541 ggctctccga ttggatgaat aactttctca ccatccacga ttacatattt cccgtcttca
     9601 tctactgaat agaacgtgag tctccctttc tcttcctcta cttcgttcac accagaaagc
     9661 cctttcgcta ctggatatcc gatttccagt ccgttaccat ctgtttcata aagttcaaac
     9721 tctgcgccat tgattggcat attactgcct tcaattagtt tattgatatt catgtggatt
     9781 ggcttgaatt catttcgcat ttccagtttg acatacaact catcatactc tgtgccttct
     9841 atgttaggct cttgtacaac agaaagcttg tgatattctc cttcataata ggaattcgga
     9901 tctttacctt ccggtccgcc gactctcgaa ccagatgtat attcatctac aagctcgtag
     9961 tgacctaaca tctcatcttc gttggctgca tattttttga tttggaattg atacgtctca
    10021 tctactaact gataacgtgg tggtccagcg atctctctga tccaaaatat gtcgctgact
    10081 gctccagttt catctcctaa aatcggcatc ttcgtttcaa agatcgctat tccgtccgtg
    10141 gtagttttac ttgtttcgat tggttcggct tccccactct ctggattttg gtcatataat
    10201 gcaaattcga ctccagccaa cggctcgcca ttttgaccct tcttgctgac ttggaaatta
    10261 cggcctttat gttcgtaacg aatggaagaa gatgggaaat acatccgtcc agtagtgata
    10321 tcattctcat aatcctccag gttctgaata taggccatat tgttcactgt gcaaaaattt
    10381 ccgtcttgag ctcccgcatc aagttttact tggtacttga tcctgaccat ttccccttca
    10441 cctaaagaaa tcggagaaaa tttataagct tggttttccc gtccgacata cgttgtcgga
    10501 gtcgccacta cttctggtgg ggcttgtcca tgacgttctt catcccatat ttctggctct
    10561 tggtacttca tgccgttttc tcctggatct agatggtaaa actgaatatc tgccgatctg
    10621 ccagctccaa atggtgagac attaaaacca gtttcatcat aaagtgtggc ttgctggatc
    10681 gttctcttga aggtagaaga gatatcctcc aaggctgtaa caatgtcatt cttgttgttc
    10741 gcattgtaat atcgcggtgt tacgccatct tcttctaaat cagcaatatt tttcaataca
    10801 tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt ttccatcgat tcctaaacca
    10861 atggagaaga tacttggcgg gttctcttga tacttgtttt tcagccaatg atgataagca
    10921 actgtgttga tcgcagatga cttttcttgc gaagtaggac tgccattcgt attgttatta
    10981 gaagaaccat caccaaccca gtgagtcgct ttattatctc cttcttttac ctctccataa
    11041 cgatagtgta catttacttc atcttctgga cggttaaagt cagatgcata ggtaactgga
    11101 tgagtaaacc ctgcattaga agtattccca gaaaactcct caaaatttct gaacaaaacg
    11161 tcattatctt cagcaatctt atcttccatc acagtccagt tgctccaagc tccattagtg
    11221 ctcgtacgat aacttgactg aatcggtgca taactgaagg tcggtgtccc atctcccaca
    11281 acgattagga ttttttctgg atttctattt tctccgccat tgtctttata caatgtttca
    11341 tagccatttt taagccctag tgtaagcgga gttcctccga ttggtgtacg atttaatgct
    11401 gtattctcac ttcccgtcaa acgattgata tcatttgtca atggaaagat gtccgaactg
    11461 atttgctcat ggttattgat agctgtacta ttatggtaaa aattcaccat accgattcga
    11521 atatccagac tcgggtcaga gagcagatct ttactcattt cgtttactgc ctcaatcaac
    11581 gcagcatttt tagtttgact attggtccca gcggttaatt cactcatact cgctgactta
    11641 tctagaacaa aaacgatatc gattggactg atttcctttt tctcgccccc gatgacatct
    11701 agatagacat tgtaaagggt tgttgcatct tcaggatgta gatcagatgc tgtgctgctt
    11761 ggcagaacag gttctaccca ttttttgatg tacgccccgt tataatccaa gtaactgttt
    11821 tcaaaattcc gttcatttcc ccaaagctga acggtttgag ctggaacgtt tctatcttct
    11881 tcgcctgcag ttgatccatt attagtagca tagtcgtaat ttcgccagtt cgctcccccg
    11941 tttgtataat cactatcatc gatcaagttc tctccgtcat atggcgagcc tttgtctcct
    12001 tgatagcgtt cattgctttg tgtccaattt cctaacactt ttgtatagtc attcgttgta
    12061 aacttcatct attctttcat caaccaccac ttgaagaggt agttaccctt cgttcacatc
    12121 ggggatcttt tctgtttcaa aaatcaatgt gccggtttct gcttcttttg aataaacttt
    12181 tccgacatac cattcttgac catcgatcgt ctcgactttt aggtcttctc catcttcatc
    12241 ataggaggat agacctgttc cacgaacgtt ttttacggta ccaatcccgg cggctgccgc
    12301 atcgattgct aaacgtattc taccttcatt ggcactttcc tttttttcaa aagccagatg
    12361 ccattcaagg gtttcttctt tttcaatata cttcccttca atttttgcat agtcctcatc
    12421 taagaatacc tcatcctcct ttgtttctgt tgactcagca gctacatcca gggtcgtcat
    12481 atgatagact gactggaaat atggtccaaa tagcaaactg acagttacta actgcaaaag
    12541 cttcaatttt aaccctttca catccttata ctcctttcaa aaatatttgt cttctttcac
    12601 aacaatcaac aaaacaatgc ccatctaatt ataatttaat ttaattttaa ttttaaatca
    12661 aatgatatcc tttttagtta aaaacaccaa ttatacaaca tttattatta aaattaaatt
    12721 ataaaatttt ataacgttct catttaacaa aacttaagtt attaagacaa atgaataaac
    12781 aactacgctt tcctcgaaac aaaaaaataa agcacaaatg cgcatttacg cactcatgct
    12841 ttattcgtac aattaattca gataacggca gcgcatagga tgccaataac acttcctaaa
    12901 atagctcctc ctaaaatgtc agtaggataa tgtaaaagta aaaaaatcct cgaataagcg
    12961 atcaaacaag caaataaaaa ggctaatatt cctgcaactg gacttaattc gaacaacgca
    13021 gctactgata aaaaggctag cgctgtatgt cctgaaggaa aagaagaagt cttcggttta
    13081 cttgtgtaaa ggacaagccg acgatttttt tcataaggtc ttttccgtct tgtcgttttt
    13141 ttgataaaaa gcaaaccaac aaaaaaatag atcaaactgc cgataagaat ggctagacca
    13201 tattctctag tatcaggtat caagactaaa acagcagcaa ggagcaacca aataaaacca
    13261 ccattagaga tattactgat aaatttcaaa aaattgattt ttcgactatc cgttttccgt
    13321 tgcttggcga acttgttgat aatactatca tcgattttag caatcaaacg caacatgtta
    13381 ttcctccttt ttaagaacct tttcttgcgt acgatggata aaaagcagga cttgaaaata
    13441 aaacaagaaa ctgaaaccta ataaagcaaa atagattggt cctgcaaacc agaatgcgac
    13501 aagaaggatc aacgtgcctg ccaaatagaa aagcgcgctt ttgaaatcat taaaaaacaa
    13561 gaaaaatgca gtaatccaag aagcttcttt ttgttccagt tgaagcaaga ttttgtaact
    13621 aaagagaagc gcgcttaata gaagcaaaaa caacgtagta tacatcaatg tatagccaat
    13681 tcccccaccc caatagcgga aaatctgata atcgatcaat agaagaagaa aataaacact
    13741 tgctaaacta tcttgctttc cataaagaga gaagttctca cgaaactttt tccagaatag
    13801 tgaaccgagc tttttctctt catttttttg taatgataaa tcatatacag aactgttcga
    13861 cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg
    13921 ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc
    13981 gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt
    14041 gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat
    14101 aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt
    14161 tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg
    14221 aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa
    14281 tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata
    14341 agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca
    14401 ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg
    14461 ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag
    14521 gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca
    14581 ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa
    14641 tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa
    14701 cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa
    14761 tgattcctat tactgtcatg aggacataac ttagtatttt ttttgtttgc cgattcacac
    14821 tggtcttttt gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg
    14881 tgcaagcttc aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat
    14941 agccatcgca taacccatat caaaagctac gaacgccttg ttccacatat gcagattata
    15001 aaaaagcaaa gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg
    15061 aaaaatttgg aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat
    15121 taatggcaaa gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga
    15181 ttcatataaa gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc
    15241 agaactccat attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca
    15301 agctggtcca gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga
    15361 aagcagctgc atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa
    15421 agctgtacgg aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac
    15481 actgaaaatc gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga
    15541 tttccaaaat aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt
    15601 catcttggat gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc
    15661 tagcataaat accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc
    15721 ttcttttttc tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac
    15781 agtcaaaaat ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg
    15841 ccaatccttc ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta
    15901 atgtcgtgta atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt
    15961 cgtccgccat ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat
    16021 tctcattggc tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta
    16081 cttctggaga agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt
    16141 atggtacttc caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt
    16201 ctgggagctg catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt
    16261 aataattgat attttgggca cgcatggcta ctaatccgga aataaatgga tcagatacac
    16321 cagacccaaa ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt
    16381 cttggatcca ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt
    16441 tgtcaaacca actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc
    16501 ataatggata aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg
    16561 ctgtttcttc taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt
    16621 taaataactt tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag
    16681 gatcaccttc atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt
    16741 tttctacata tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat
    16801 ttatgtcctg tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag
    16861 tccagatgtc accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat
    16921 tgaaatcctc aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc
    16981 agaaatcaat attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac
    17041 tggcatcgct tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca
    17101 gcaaccctgt aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc
    17161 cggttttaat ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct
    17221 aaaaaaatta ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt
    17281 ttaatctttc gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt
    17341 tagacaccag aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc
    17401 gaaatatctg caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta
    17461 cctgttccaa ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat
    17521 tgcaacagaa gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga
    17581 gggtgcgcct gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat
    17641 tatctctgga atgaatccac agcacgcgat cgtagctggc ttaggacctc gtcctgctga
    17701 cagcgtgggc aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga
    17761 ctttagagct aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga
    17821 aatgatcgat gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta
    17881 tcatcaaaat ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt
    17941 agtcccaagt actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa
    18001 cttctcagag ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg
    18061 cgaaggcgaa tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga
    18121 accagtaaca ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag
    18181 aaacacccgt tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat
    18241 aaccaccctt caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc
    18301 ctaaagacgc tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc
    18361 acctcttaaa aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta
    18421 cactcagctt atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg
    18481 tcgcttcatt ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc
    18541 caaatttata ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat
    18601 tgattataaa ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc
    18661 tactttgtac gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg
    18721 tatttcaatc gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg
    18781 cttcactgac agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg
    18841 caatctattt tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa
    18901 taaagccgta taaaaacaag aacctatgaa atctttattt attagatatt caacagaaat
    18961 cccgataaca ctagccaaca atatatttat cagaagtgta aaataaggat gctttttata
    19021 gaatagaact agtgttgttt tcatagactc tcctttcttc atatttccaa ttaatcacaa
    19081 attcacggca ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt
    19141 tccctacaaa ccaggccatc ttgtgtttaa taactatacc aaatctaagg tacctctaaa
    19201 tctaaattcc atttgtggcg gtgacctcca atttttaact cttctatctc ccatccagta
    19261 tacataacct tgatacttcg accacttata tatcgaacac acaaatacct atccattgca
    19321 ggagtactac ctaacttagt tgctacttgc atctaaataa cagctgaaaa gataggactt
    19381 actcttagta aagtatctga actattaatt tttaaactat catttttatc ctcaactaca
    19441 tgcacatctg ttgcaacttc actttctgtc tcatccagat aaatgattaa ggaatcggga
    19501 attgcaatat cttcagctga cgctggaata gttgagctaa attagtcatc atatctttat
    19561 tataaaataa ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt
    19621 aataaatgtt gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat
    19681 ataccctata aaagctaata agatggttag gaatccttta gaatttttac tcattcatag
    19741 acacttcctt aaataacttt tattatagaa ggtattgtaa ataataatat tgacaaaaag
    19801 tgactcaaaa taaaaaaaaa cttacattat actggaatga actgtattga tagtgtacct
    19861 aaaagctcta ctgacataag atcgtgtaat cggtaaagta attttattaa ttatacggga
    19921 tgtaatcaat tagagaataa actaatcata aaaggtactt ttacattcat aattgtcgct
    19981 ttgagattct actttggttc actatctgac ccaattgcac attttcgatc attacaagta
    20041 ttggtgtcgt tagaacaact aaataaaaac tagcggtaga tttttcagaa tctatgctaa
    20101 tttctgtatg gatgttccag tgatggcaaa tttctctttt cattgttttt cttgtcgcat
    20161 gatatgcctt tttgtgttat tcagttaaac tatagaaatt ctttcctaga cagatcttga
    20221 tcccaaattt tacgtttttg agcatcgata tagtttaaaa tgacattact catatctaaa
    20281 gagtttagat tttttaggac taaaactgtc agttgttctt tttcttcttt ctgttaaagt
    20341 attgtcgcga cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca
    20401 aaaaaacgaa tgacttgagg aaatcacctc aatcattcgt ttaaaatgct atatattctt
    20461 tagtttaaga tacggaaatt aatgccatcg accacactag tagaatgaac gacatttact
    20521 tggccttttt ttaatttcgt tatgtttagc atataaaaat attatagagt ttacttacaa
    20581 tgcttaaaac atatactgta taatctgatc aattttagcc atgtcttgca atggtaagtt
    20641 ttctattttt ttgagttttc gttccttaaa atcaagagat tttaattgtg taataattac
    20701 ttgtcctttt atatctagct cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt
    20761 ggaagtaatt ggacatatga ccgaaaacat tgttttacgg ttaaattcat aacgagagac
    20821 gactagtcct ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa aatcaatcca
    20881 tacaatatca ccttttttag gaatataaat tctttcttct gacactataa aatttctctc
    20941 ccttcaggag ttaaatcttc ccattcgtct ttctcataat actcggcctc ttccccacca
    21001 ctaaatggat cttcaatttt tggaactaac gtaattgttc catcatcaga atacacaacg
    21061 atatattctt ggttttctga aggtttcttt ccgttatcag aaggtaaagt taccactaca
    21121 gaacttcctt gtaatctaga ttttgtaaca agcattatca tcactccctt aataaaagta
    21181 tactatataa cactaagtaa cgcaaaattc ccctttgtaa actatctaga aaattgtttt
    21241 gtttaaagta tatcttattg gatataaata gaaataaatt gtagccagtt cattttttct
    21301 ttaactaaaa aaataatcca agaaacattg aacctatgga tccaaaagct acccaaatac
    21361 tatagctaac cccccgctac cttcaatttt gcagataaaa cgctttggga gaaaatttca
    21421 aagacaacag gccatcctat catgcccgat tataaacaaa aagctggcta ttccttttgc
    21481 tctgacaaat ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt
    21541 cggatcaaag aatgcccgct tcaaatagaa acattggttc aacgaatttc aactagacaa
    21601 gactttgcta ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat
    21661 gatccaactc atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat
    21721 acaactgcca accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa
    21781 tgataaacat cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga
    21841 agctatacat tagaaaggtc gactgtgacc ctatagatgc agtatatgca gcttatgagc
    21901 atttcttctg ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc
    21961 tctttctttg aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg
    22021 agtaatgatt tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt
    22081 caagacaagc ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat
    22141 tatgattgat tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac
    22201 tattttttcc ttatttgaaa acataaattt gtctttttct tcattccact cattgggaaa
    22261 acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac tgtctctgca
    22321 cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt catgatttcc
    22381 ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga cggaaaaact
    22441 tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc tcgataataa
    22501 ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct aatccctccc
    22561 aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac gctttcaatt
    22621 tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg tattctcctg
    22681 ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct ttttgaaatt
    22741 gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa
    22801 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcta ttttcacaaa
    22861 atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc
    22921 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca gctattttta
    22981 tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc actaaacgat
    23041 gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    23101 gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct aatacacggt
    23161 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt gagaagcaat
    23221 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctaattatt
    23281 aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta
    23341 gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca gcaatcaacg
    23401 ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt gccaggatat
    23461 tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga cggtattctt
    23521 catccactgt actcaactca ataatcaaat tacctacagg acagccatga taagtgatct
    23581 gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct tctggctgtt
    23641 ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga
    23701 tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga ccttttcctg
    23761 tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt ttataaataa
    23821 gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc ctttttcatt
    23881 tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc gtttaaaaaa
    23941 gactgtgcca aattatctga cacagccctc actataaaac tgttctcata tagctagatg
    24001 ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata gttgctctaa
    24061 tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca acgctgcatc
    24121 cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat tgaatccact
    24181 ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc caccaagaat
    24241 tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa
    24301 gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca attcttcgat
    24361 aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag caaacatctg
    24421 ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt catgtgggta
    24481 aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt ttaccatttc
    24541 aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac caaagccacc
    24601 gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg tatttttatt
    24661 attgaacatg gtggattctc cattcgttat ttattttaat acatcattta gagcttcact
    24721 cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt ttccattcat
    24781 agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac ttgcccctag
    24841 aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat cttctaatac
    24901 ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga cgccttgtgc
    24961 tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa atacactgta
    25021 aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact gatccataat
    25081 gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga cgtctccaat
    25141 cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg ctccgcgatc
    25201 tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt ttcttccagt
    25261 tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg taaaggtgac
    25321 ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat ggaattcgat
    25381 tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag
    25441 aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga acattgatgc
    25501 aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta attgttcctg
    25561 atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt tgattggtaa
    25621 aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa tcactttttc
    25681 accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac
    25741 tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa
    25801 tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa
    25861 tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac gtgttgactt
    25921 ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag tcttaccgcc
    25981 ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct cgcttctttt
    26041 ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc caaatcatct
    26101 gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt agtctggttc
    26161 gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct ctagttgctt
    26221 tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga
    26281 tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca tctaaattat
    26341 aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct ttgatcatat
    26401 gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt tctggatttg
    26461 ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca tttggtgcct
    26521 cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct tttttcattg
    26581 tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta tgacaagtaa
    26641 ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc tactattttt
    26701 ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc aaaataaata
    26761 gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata taccagaaag
    26821 ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat tttttcctcc
    26881 ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg ccgtccaagc
    26941 aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat cagaaaaatg
    27001 tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt catcattttc
    27061 tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc
    27121 gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga agttcatccc
    27181 tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg
    27241 ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt tcaactcttt
    27301 gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca tcaggatctt
    27361 tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag cgtgtccgct
    27421 tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg ctttattgat
    27481 tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt caatgaatct
    27541 ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat gctcttttaa
    27601 tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc cagaagcact
    27661 gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg cttcatttgc
    27721 taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg ggatatgatt
    27781 aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt
    27841 caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa tgattttcca
    27901 accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg tgactaaatc
    27961 ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt cgctttttat
    28021 tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc cttctgaggt
    28081 aaagaatttt attccattat aataataggg aagatggcta gcagtcagca taaccgcagc
    28141 atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag tagctaattc
    28201 gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct ctttcagtgc
    28261 tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat ttggatcggt
    28321 tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt ttaccatgtc
    28381 tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa tatccgatcc
    28441 attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc cctcattttt
    28501 tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat aatgaatgta
    28561 tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag ccaattcgtt
    28621 accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac agactggata
    28681 cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga aacagatggc
    28741 tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac taacttcatt
    28801 tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc
    28861 gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg gatcagtgct
    28921 tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca aaagataagt
    28981 aatatttctt catattttgg tattttttac ttttattttt tgttttcatt caggttgcta
    29041 acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg attttcttgg
    29101 atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt cttttgcgat
    29161 gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga cgtgtaagat
    29221 ttgggcacaa agaccttctt ccatcttttt gaatactact tgttctttca agtcttccgg
    29281 aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa agtcaggctg
    29341 tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat gcaaatactt
    29401 ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat aatcaggaat
    29461 cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata gacaggctac
    29521 tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa agaatgattt
    29581 tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt ctttacgcca
    29641 ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta ttatcatacc
    29701 tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg ctagaataag
    29761 tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag gcggtttcta
    29821 tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga tgtaaatgac
    29881 aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga tgtcttttgc
    29941 gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca gacagtttga
    30001 tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga tttcgatcat
    30061 tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc
    30121 aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct tcatggtcct
    30181 attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt tgatgttcca
    30241 tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt
    30301 gacaacggcc attgctccag caattggccc cacttatgga ggcattatga cttcacattt
    30361 tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt tgattatcgg
    30421 ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt ggataagcgt
    30481 tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt tgggaacagt
    30541 ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt actatcgaag
    30601 taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa cattccgctt
    30661 gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt ttgtcttgcc
    30721 gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag cgatgcttcc
    30781 cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg atcaatatgg
    30841 tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc tagcattatt
    30901 cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt tttatatgat
    30961 cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac tgaatgaaga
    31021 actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg gcgctgtggc
    31081 tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc ataactatga
    31141 agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt
    31201 aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga ttccatatac
    31261 ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt
    31321 tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgaccct agccagttac
    31381 ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca ggcaactaaa
    31441 gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca atggtatcgt
    31501 ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca acttgatcaa
    31561 acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt agctgaattg
    31621 gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt
    31681 tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc actgcgtatc
    31741 agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca gcgtttagca
    31801 caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag cgattcttgg
    31861 ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac acctggcccg
    31921 gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc gcaaaaaggg
    31981 cagctcttag catttcagac atcttataag atgagtcaag actggcctgt tgctatgctt
    32041 gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg tgctacccat
    32101 gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg attgactaag
    32161 agtacagaac agttcttgac tgatccagat cagataaaaa actggccata cgagttccgt
    32221 gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact gccagatgat
    32281 ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg tccttatatc
    32341 ggctatctat tagccaactt cttaaatact ggtcattggc ctacggaaga ttatcaaaag
    32401 gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt caagtaagga
    32461 tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta gttcaatttc
    32521 tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt tcctatctga
    32581 gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt ataggacgta
    32641 tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc aaatggacca
    32701 aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa tgtcgtttca
    32761 ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc tgtttgctgt
    32821 ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac acggaaagga
    32881 ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat attttccgcg
    32941 tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg gtcattgatc
    33001 ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc gatcttttcg
    33061 ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg cccatttccg
    33121 tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt cacgcccaca
    33181 ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc ttgtgtttta
    33241 tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat aacgaaagca
    33301 tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt aatcgttgat
    33361 tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt actagcatat
    33421 tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc
    33481 gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc cgctgtatta
    33541 tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca ttcgggatca
    33601 caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt ctctaagatc
    33661 ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc aaatccttct
    33721 ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat tacttttgta
    33781 tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt ctcgtatttg
    33841 ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct tcccacaatg
    33901 cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc ttcagcatgt
    33961 gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg ataagctgtc
    34021 ttcctatcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac aatcgatcga
    34081 atactggata acagtggatt tccacttgtg atttcgttta attcgagact ctcttttgta
    34141 ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata cattattcga
    34201 gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac
    34261 cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc
    34321 agccgtttgc taaccagaat gttttagcag catcccatga accataacga gatgttacat
    34381 attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat
    34441 ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt
    34501 tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag
    34561 caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt
    34621 cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg
    34681 aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt
    34741 tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata
    34801 atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa
    34861 acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta aatgaattca
    34921 tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact ttatcattta
    34981 gaaatatttc ataggtaaaa gaa
//
LOCUS       DACDIP010000021        34909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDIP010000021 DACDIP010000000
VERSION     DACDIP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.021"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            423..581
                     /locus_tag="IV257_001238"
     CDS             423..581
                     /locus_tag="IV257_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8814381.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            653..1879
                     /locus_tag="IV257_001239"
     CDS             653..1879
                     /locus_tag="IV257_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8814382.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(1968..2360)
                     /gene="rpsI"
                     /locus_tag="IV257_001240"
     CDS             complement(1968..2360)
                     /gene="rpsI"
                     /locus_tag="IV257_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8814383.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2376..2819)
                     /gene="rplM"
                     /locus_tag="IV257_001241"
     CDS             complement(2376..2819)
                     /gene="rplM"
                     /locus_tag="IV257_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8814384.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3338..3805)
                     /locus_tag="IV257_001242"
     CDS             complement(3338..3805)
                     /locus_tag="IV257_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8814385.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3925..5463)
                     /locus_tag="IV257_001243"
     CDS             complement(3925..5463)
                     /locus_tag="IV257_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8814386.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLSGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5543..6229
                     /locus_tag="IV257_001244"
     CDS             5543..6229
                     /locus_tag="IV257_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8814387.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6430..6957)
                     /gene="msrA"
                     /locus_tag="IV257_001245"
     CDS             complement(6430..6957)
                     /gene="msrA"
                     /locus_tag="IV257_001245"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8814388.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7041..7718)
                     /gene="pgmB"
                     /locus_tag="IV257_001246"
     CDS             complement(7041..7718)
                     /gene="pgmB"
                     /locus_tag="IV257_001246"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8814389.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7715..8845)
                     /locus_tag="IV257_001247"
     CDS             complement(7715..8845)
                     /locus_tag="IV257_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8814390.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8845..11028)
                     /locus_tag="IV257_001248"
     CDS             complement(8845..11028)
                     /locus_tag="IV257_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8814391.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11025..12050)
                     /locus_tag="IV257_001249"
     CDS             complement(11025..12050)
                     /locus_tag="IV257_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8814392.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12095..12922)
                     /locus_tag="IV257_001250"
     CDS             complement(12095..12922)
                     /locus_tag="IV257_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8814393.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12919..13986)
                     /locus_tag="IV257_001251"
     CDS             complement(12919..13986)
                     /locus_tag="IV257_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8814394.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14012..14848)
                     /locus_tag="IV257_001252"
     CDS             complement(14012..14848)
                     /locus_tag="IV257_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8814395.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14845..15714)
                     /locus_tag="IV257_001253"
     CDS             complement(14845..15714)
                     /locus_tag="IV257_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8814396.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15784..17067)
                     /locus_tag="IV257_001254"
     CDS             complement(15784..17067)
                     /locus_tag="IV257_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8814397.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17081..18766)
                     /locus_tag="IV257_001255"
     CDS             complement(17081..18766)
                     /locus_tag="IV257_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8814398.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19041..20066
                     /locus_tag="IV257_001256"
     CDS             19041..20066
                     /locus_tag="IV257_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8814399.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20661..24314)
                     /gene="rpoC"
                     /locus_tag="IV257_001257"
     CDS             complement(20661..24314)
                     /gene="rpoC"
                     /locus_tag="IV257_001257"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8814400.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(24393..28010)
                     /gene="rpoB"
                     /locus_tag="IV257_001258"
     CDS             complement(24393..28010)
                     /gene="rpoB"
                     /locus_tag="IV257_001258"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8814401.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28738..29706
                     /locus_tag="IV257_001259"
     CDS             28738..29706
                     /locus_tag="IV257_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8814402.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29724..30629
                     /locus_tag="IV257_001260"
     CDS             29724..30629
                     /locus_tag="IV257_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814403.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30626..31432
                     /locus_tag="IV257_001261"
     CDS             30626..31432
                     /locus_tag="IV257_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8814404.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31589..32557
                     /locus_tag="IV257_001262"
     CDS             31589..32557
                     /locus_tag="IV257_001262"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8814405.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32874..33398)
                     /locus_tag="IV257_001263"
     CDS             complement(32874..33398)
                     /locus_tag="IV257_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8814406.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33685..>34909
                     /gene="scm"
                     /locus_tag="IV257_001264"
     CDS             33685..>34909
                     /gene="scm"
                     /locus_tag="IV257_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8814407.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDS"
BASE COUNT         9982 a         7409 c         6536 g        10982 t
ORIGIN      
        1 tgataattta tcttaacaac cagataattt tattcagcac cgtattatct atattgaatt
       61 ctcggaatca agataaattt ttttccgttt ttgtttcttt tttaatcttc ttcttgttat
      121 taatagtgat taggatgata ttcctatatt gatctttgat aattagatat aatttttgaa
      181 taacgttccc tgctattcga gctagaaata caagtacgcc atgacctagt tagcgacaac
      241 tacttagaga tttcttttgt tctgggacaa caaaatgcca tgacaatagc agtaaataat
      301 gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg tgcttaccca
      361 aagcggttca aaaattttaa actattattt taggagatga ttttataaaa tcgaaagcct
      421 acttgaataa aaaacaagcc tgtgaatttc taggaattga taataatact ttagataagt
      481 ggattcgtgt atataatttt ccagtaataa aaattgatgg cgtagttcgc tttgacgtag
      541 aggatatcaa agatttcatg aatacattca aaaaaaattg aaattttctt ctcgtcacat
      601 atactataaa tagcgaaatg tactgcctta ctttttaaag gagtgaggaa gtttgcctac
      661 aaagctttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat tcagagaaat
      721 cactggaatt gcatcagaga agtatcaaaa agtatttcca actaggcaat tagctattaa
      781 ggccgaaagg gaaatgaaaa agaagattga aacagtttta agagaggaga atgcaaactc
      841 actagaattg aaaggaaaaa taaaatttaa agatttttat gaatcaaaat ggcttcctag
      901 atatgaacta ggtcaaacta ctaggtcaaa cagggctcca agtaatgtaa caattagtaa
      961 tactaaggat atattccgat tgcacatcct tccaatgttt ggtgaatatg caatgaatta
     1021 tctgaattca aatactgaga taatatcaga tgaattaaca aagaaatcga aagaatacgc
     1081 aaatataaaa attatcaaag gctatgttcg ctctatgttt gatattgccg aaatactatg
     1141 ttatatcgaa ttcaaccgaa ctaccaaaat tattcaatgt attaccgcgc ctaagaaaat
     1201 ggctttagaa gaaaaaagga taagggaagg gaatcaagca ttatcatcag aagagctgac
     1261 tgattggata gaagcagtaa acaatgactt aaataatcac ttgcttacac ttcacgatta
     1321 cacacttttc atgcttacct tatatctagg tgatagaaaa agtgagactt atgctctgca
     1381 atggaaatat attgatttta ataaaaatac ggttcgtctc aaatatgctt tagataaata
     1441 tcaaagaaag acacatacaa aaggttggaa agacaccgtt attcaggcac cagaagttgt
     1501 gatgacatta ttaagagaat ggaaatcagc tcaggctgaa caattgttga aactaaaaat
     1561 tatacaaaca cctgaccagt acctctttac ctattccaaa ccttctggag aagtaaattg
     1621 tcctgttcac gctgactact taaactatcg aatcaatgca ataaaaaaaa ggcatcctga
     1681 attggctcaa ttatctccac acaaacttcg tcatacctat gcaactatag ctagggaagg
     1741 tggagcaaat atgaatcaaa tctctaatgc tttaacgcac tcggatattt ctactacaaa
     1801 aatatacgtg aacacacctg acgtagttga taaatctgtt tttgaagcat ttcaaaaagg
     1861 attgaataag tgtgattaac atgtgttctc aaatgtgttc ttattataaa aaaagacact
     1921 ttccaaataa ttgaaaagtg tcctgaaata ttgatataaa aagaatatta acgttttgag
     1981 aattgtgaag ctttacgagc ttttttaaga cctggttttt tacgttcgac catacgtgcg
     2041 tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc aacttctagc
     2101 aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc tccgccgttt
     2161 acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt
     2221 acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt tacagtaatt
     2281 ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg
     2341 atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt gatgtctaaa
     2401 acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt caattttgta
     2461 aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt ttcgatcaaa
     2521 cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc tcctgggtag
     2581 ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt cactttgtct
     2641 gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt tggtttattt
     2701 ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac atcagttgcg
     2761 tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt tgtacgcacg
     2821 ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc cggggctctt
     2881 tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg tcaacgtttt
     2941 tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt atataaaagg
     3001 gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc
     3061 cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat
     3121 aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga acggcaggct
     3181 tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
     3241 caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt
     3301 tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc tctggcgctt
     3361 tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg aattcgataa
     3421 agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct ttttggtaaa
     3481 gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga atagtacgat
     3541 cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc aatgtagaat
     3601 aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga gcgttcaaat
     3661 catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct ctcatgtacc
     3721 aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat tgatttaata
     3781 cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt acacttaaaa
     3841 taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac gattttcagt
     3901 tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt ctcgcagttg
     3961 agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata atggatagat
     4021 tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca attctatcaa
     4081 ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta aaatcacagc
     4141 tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca gtacatcagc
     4201 catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga tctctgtgag
     4261 ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga tcccttctag
     4321 aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa aactgccaaa
     4381 cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa aaatcagacc
     4441 attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg gattttctcc
     4501 tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc aatcaaagat
     4561 gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca gaacataaca
     4621 aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa gtgctccgct
     4681 cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta gattagaaag
     4741 cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta gttctgctgc
     4801 tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg gcaattgctc
     4861 tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat ggatatcaaa
     4921 atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccagaca atctataaaa
     4981 gatatagtct ttgataccga caaaataagc agtttttttg aataattcag gttggctgtt
     5041 ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc cagtctgccg
     5101 ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg ctgctcggtt
     5161 atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca atatcaggcc
     5221 atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca actgtttaat
     5281 tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt gttctgccat
     5341 cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt cttgggcttc
     5401 atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga ttgaaacgtt
     5461 cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt gcgtatcttg
     5521 gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct gtatcggatc
     5581 atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt atcctagatc
     5641 tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga ttctctcgat
     5701 catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct actatttgtt
     5761 ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt ccgcaagaaa
     5821 tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg atttatttta
     5881 cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga ttttttggtt
     5941 gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg
     6001 ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc tttcttggag
     6061 tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc tgtttggaat
     6121 atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat
     6181 tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa aaaaggttgt
     6241 gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc cttgtgactt
     6301 ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag ctgaccctca
     6361 gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat aagtagcttt
     6421 ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac gtccagaacc
     6481 ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc
     6541 tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat cgaatcgccc
     6601 gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt catcattata
     6661 gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg
     6721 atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt ctggatcata
     6781 ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt cataagttgg
     6841 atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg gctgctcttc
     6901 aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata
     6961 aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg ttttgcttgt
     7021 gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg ccaaaacgaa
     7081 cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg aagatttatg
     7141 ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca accagcttct
     7201 gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc tcctttgaga
     7261 aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa
     7321 taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat
     7381 ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc aggcaaaacg
     7441 gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg ctcagcaaag
     7501 gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag caatcgctct
     7561 agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt gaatggaatg
     7621 gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt gtctacaaga
     7681 acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct tgaaaatgaa
     7741 cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca gtggcttgct
     7801 gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa
     7861 catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc acaggaacgc
     7921 cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt tgtccatcta
     7981 gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg aactccagta
     8041 aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct aacagtcctg
     8101 cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg gcaaatctca
     8161 tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc aagccttttt
     8221 gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta acatcagtaa
     8281 tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca actgtagcaa
     8341 ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg taaaaagcat
     8401 aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg ccaacgccga
     8461 tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct actcctcgcg
     8521 tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg acgatttctg
     8581 ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg tttttgccaa
     8641 atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct aatgatgaaa
     8701 ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga acaaaaactt
     8761 cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt tctttgaaag
     8821 aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca gttctttttc
     8881 cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct ctttttttat
     8941 tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa taaaaggaaa
     9001 agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc cattcgttac
     9061 tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg aagcagcatg
     9121 aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg cttcttggaa
     9181 taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt gaatcgcttt
     9241 actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat
     9301 tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg cttgcttgct
     9361 gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact ggctgccagg
     9421 acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt catcttgcgg
     9481 aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat tttgacattc
     9541 tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat tataatgcgc
     9601 cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg gaccaatgac
     9661 tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa aagctgctgt
     9721 ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg ttgcttggac
     9781 atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg cagctgaagc
     9841 aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg tctcttcctg
     9901 accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat ctcttgcttt
     9961 ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg ctacttcagg
    10021 aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa catgcccttt
    10081 ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat tgcggggagt
    10141 cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc
    10201 ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg actgctctaa
    10261 aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa acgggtctgt
    10321 ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg tattttttct
    10381 gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata cacctgcttt
    10441 attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt aggatgcact
    10501 gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg taccaaaatt
    10561 ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg tcaacggctt
    10621 gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac gacaagtaat
    10681 gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa gctctcccat
    10741 ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag aaaaatgttc
    10801 accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact cggctgattc
    10861 tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt gattccgata
    10921 gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca tctgactcgc
    10981 atacttattg atatactcta aatcaaactg tttttcttct actttcataa ggtaataact
    11041 tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac atatccttgc
    11101 cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa atgagccata
    11161 cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag ctttccatct
    11221 acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc acaaaaagcc
    11281 acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg gatgatcttg
    11341 ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc
    11401 catttcccga atttccctga attttttttc gtaccaattt tttggaagct gtacgcccga
    11461 acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat gtgaatgcca
    11521 taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc ccagcctgga
    11581 acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag aacatcaaga
    11641 tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata agccaaaatc
    11701 tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt catagctggt
    11761 ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg ttcgaagtga
    11821 aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag catttgtgta
    11881 actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg
    11941 actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata ataagcagca
    12001 tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat ttatttctcc
    12061 ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa tcatttaaat
    12121 gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa attttcactt
    12181 cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca ttttgaagta
    12241 gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag cgatgattat
    12301 ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt gaaatatcat
    12361 cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc aattgattag
    12421 agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg tatcggttca
    12481 aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa taacgaagtg
    12541 attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc
    12601 gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca acctcgctta
    12661 gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct tcgataaagt
    12721 caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct gtttctttga
    12781 ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt tcgtattctt
    12841 caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg aaaaatgctt
    12901 gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca ttttgatact
    12961 ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg caaatggcac
    13021 aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt cccaacatgt
    13081 gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac tagatgctcg
    13141 agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa acccagctgg
    13201 aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc ctcttaaaga
    13261 agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc cacgtttatc
    13321 tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg tgtaatcggc
    13381 accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa cgatggtagt
    13441 agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta aaccgaagat
    13501 tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag cgaattgagc
    13561 aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac gtagtttata
    13621 attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc agactaactc
    13681 tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga caatcatatt
    13741 tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc ttggcaagaa
    13801 taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc taaagtcgac
    13861 aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt cgttcttttc
    13921 gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg caactaattt
    13981 tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct tcgactaaat
    14041 cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa gcggtcacca
    14101 atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat gtgttcaaac
    14161 cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat aaataatcat
    14221 tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata gccaatggta
    14281 taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct gcttcttcaa
    14341 tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta ggaagtgttt
    14401 gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga ccggaaatga
    14461 ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg taaaagctag
    14521 cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc
    14581 caatcactac tgcagttacg gaggctatca aggagatcac taaactattt ttgaaataca
    14641 tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa gtccattctt
    14701 tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat gatgtcatca
    14761 gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata
    14821 agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg agagcaagac
    14881 gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc caagatcatt
    14941 gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat tcgtcagtag
    15001 atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca cagttgctag
    15061 tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc
    15121 catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat
    15181 aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga agctagcaac
    15241 tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc
    15301 attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca aagaaataga
    15361 tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg
    15421 gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa ctaagaatgt
    15481 gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa tttcaagata
    15541 attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat cgaaaaaact
    15601 gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa gcatgcttgg
    15661 tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat tcattgaaac
    15721 ctccccctga taatctgcca atagctatct acttttttcg aacaccattg gcagaacttc
    15781 ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt cgtttccgac
    15841 agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc ccattttcgt
    15901 gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac tagcaatctc
    15961 agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat ccagttcgac
    16021 tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact tttccatgtt
    16081 tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag
    16141 accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt tttgtgttgg
    16201 gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca aaatgtaagt
    16261 tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt ctgtcacatc
    16321 attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg cttctctcat
    16381 cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc catttgacaa
    16441 agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca tagcaattcc
    16501 atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt
    16561 ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa taccttgaac
    16621 ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa caaggttttt
    16681 ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga ctgccgtctg
    16741 atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt ccattacttc
    16801 tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg attcttctac
    16861 cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa ccagttcatt
    16921 gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg ttgtctgttg
    16981 tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag tagctagcag
    17041 acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt attttcagcc
    17101 acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg ataatttgtc
    17161 cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat tgattggaaa
    17221 aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg ataaaagaat
    17281 ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct ttttttcgaa
    17341 tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct gtctgcaact
    17401 ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc tctcctgttt
    17461 tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa attgccggca
    17521 cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt ctccattccg
    17581 tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca tatggactgg
    17641 tcgttccatc tgggtttttc ttgtagttta ctcgggtacc ctctttcttc agctgttcca
    17701 ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat ccgataccgt
    17761 catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg atggttttta
    17821 accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc aaaggtggta
    17881 gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa ctgatattat
    17941 ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct tcatcagcga
    18001 ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa gaagttccta
    18061 gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct ttttctaagt
    18121 aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa ttcaagtcta
    18181 cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg tcaatgaacg
    18241 ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta tccagtggca
    18301 aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga aaccaagtac
    18361 tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg cttctcattt
    18421 cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt acaacggaga
    18481 aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta aatgtatctg
    18541 ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa
    18601 attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct
    18661 ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg gttttctcag
    18721 acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga ttcccctttc
    18781 ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac tgtcaatagc
    18841 atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta tgtatcaata
    18901 attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt cctaaatttt
    18961 caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa aacgctccca
    19021 aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa aaagctaatg
    19081 tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct gataaagcaa
    19141 aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa attgcaagag
    19201 gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact tctcatacga
    19261 cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa
    19321 ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta gaaaaatgcg
    19381 cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct acgatggatg
    19441 aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc ggaaaagtcg
    19501 gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat agttacgatt
    19561 tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat gctccgacag
    19621 aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt gatcaccacc
    19681 tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat agcatggcag
    19741 caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct accgatgcat
    19801 taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata cctgatgatc
    19861 tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca cagttgactc
    19921 ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta ttccaactaa
    19981 tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt actctcacag
    20041 attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa cactgaggat
    20101 aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct cccggtattt
    20161 tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta taatgtttgt
    20221 gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg tgtataagtt
    20281 tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta tttggggata
    20341 cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt ttattcgagg
    20401 aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc ccatcaattt
    20461 attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc ttggaagatg
    20521 atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt cccacttttc
    20581 ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa taatttgtca
    20641 caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc tgcagccatt
    20701 tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac ttcttttggt
    20761 tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc tataataaca
    20821 ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca
    20881 cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa tgatgctttt
    20941 gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg
    21001 tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat atctgtatca
    21061 cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc gatatgcttg
    21121 tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag caagtagttt
    21181 tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga tccttctgtc
    21241 aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc tgtataagga
    21301 acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt acgtgttgca
    21361 ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc ttgccctttc
    21421 ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat atcgtctccg
    21481 gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc accgattgat
    21541 tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt tgcaaggttg
    21601 cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac agaacggatt
    21661 gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga aatcaattgg
    21721 tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg ggtgtaacgt
    21781 cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat ggcttggatt
    21841 tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg ggccacgtca
    21901 accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc ggtcatccct
    21961 ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc acggaagttc
    22021 gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc acgcattccg
    22081 gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat catgaagatt
    22141 ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt tgcagcattc
    22201 caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg acggaattgt
    22261 tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt ttcattcagt
    22321 acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt gtaccctaag
    22381 tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt gaagacttct
    22441 gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg ctctttgata
    22501 tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg gacggtcaag
    22561 ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc gttgaagatg
    22621 atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa tggtttttca
    22681 cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc gttgcgccat
    22741 gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg tccttcttct
    22801 tccattgtca agtagtagtt acctagaacc atatcttggg aaggagtaac aactggttta
    22861 ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc ttctgcttgt
    22921 gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc ggcattataa
    22981 gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac tggttcgaat
    23041 gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg gtgttccttg
    23101 attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt acgtttcgca
    23161 tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa tggtttgaat
    23221 aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa aggtccaaca
    23281 acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg acggaaacga
    23341 ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc tggtccagta
    23401 actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg cagcatacgt
    23461 ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt caaacggtta
    23521 ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct
    23581 tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc cataaccatc
    23641 cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg gcggattgca
    23701 cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc tgcttctgca
    23761 tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc agcttggaat
    23821 tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc
    23881 tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat gatttcttcc
    23941 aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc tttaaagtac
    24001 cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg gcgaactttc
    24061 gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg gatacgtttg
    24121 tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc acagaacaac
    24181 ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt tacttcaccg
    24241 taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact ttcgaattta
    24301 tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc aagacggctg
    24361 atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc tgcggtttca
    24421 aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt cttttctaat
    24481 tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg
    24541 tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa gacacgcatg
    24601 tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg tacacctggt
    24661 tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc gactacgtca
    24721 tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc
    24781 catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc caatggttgt
    24841 tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc gaccatgtga
    24901 gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg ttcacctgta
    24961 cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg aactgtttcc
    25021 catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat acctaactga
    25081 cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg tgaaggcacc
    25141 cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg catatcttct
    25201 tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat tttatcccct
    25261 tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac gcctggtgac
    25321 aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc gccgccgcca
    25381 tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa gatcgcatgc
    25441 aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt ccctacaagc
    25501 aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc taaatctttt
    25561 aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc taattttgta
    25621 tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc atcttttacc
    25681 aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt catgaaacca
    25741 actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc gtctgccaga
    25801 gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt
    25861 ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg
    25921 cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt acataacaaa
    25981 gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca tggtgcttga
    26041 ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc acggttggag
    26101 tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg tttaggtgat
    26161 acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg agcacgagcc
    26221 ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc ttgggctacg
    26281 atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac acgacctgtt
    26341 tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac tttcgcataa
    26401 ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat cggacacata
    26461 cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc acgagtcaat
    26521 ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag agggtttgtt
    26581 tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact tgccacaact
    26641 ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat cgacattctt
    26701 tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg taaaagttca
    26761 ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt gccgatacct
    26821 tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg aaccgttttt
    26881 acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg gtctcttggt
    26941 gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc ttctgatgga
    27001 tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc catgacttgg
    27061 tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc aactaatgtt
    27121 tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt tactttgtaa
    27181 cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta
    27241 cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa
    27301 ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt
    27361 aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa tcctaacgca
    27421 cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata agagatgtct
    27481 tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt agaaccgaat
    27541 ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga acggactaac
    27601 tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc tgtcattaat
    27661 gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa
    27721 cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc ttctgctact
    27781 gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt gccattgaag
    27841 tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa gaaccattgg
    27901 taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact gattcttgcg
    27961 aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa gctcttcacc
    28021 cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata tttcaaatat
    28081 tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag
    28141 aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat attaaagccg
    28201 aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca acttcatata
    28261 ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt tttcgcaaca
    28321 ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa ttgacagacc
    28381 tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta tgcgaaccaa
    28441 catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact ttagataggt
    28501 ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg atggctaaag
    28561 aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga gcagtgaaaa
    28621 tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact tttcaatcca
    28681 tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga aaaagaaatg
    28741 aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg aggttgcgca
    28801 caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt attgcaactt
    28861 gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt agcagaagaa
    28921 gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa caatcaagac
    28981 aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct acttggaatc
    29041 gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat gactgtcaca
    29101 gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc tggcggcaat
    29161 gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc
    29221 gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa ctctaagatc
    29281 caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca
    29341 gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga
    29401 atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca
    29461 aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga cggcggtcta
    29521 gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa cagggatcat ggcagcaaaa
    29581 atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga tttggaactg
    29641 tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat caaagaagct
    29701 gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta tcatctgcat
    29761 cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc cgtatcttgg
    29821 acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc gcggcagtaa
    29881 gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta gggggtgcgg
    29941 cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc cttcttgcag
    30001 ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt gcgccgaaca
    30061 tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc ataacaaaag
    30121 acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt
    30181 tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat ctggcaatga
    30241 gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg atttctaatg
    30301 gatgcattgc tctttctggc tccttgcttg cgcaaaacaa cggctttgct gatttaaatt
    30361 ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa gttttgttac
    30421 gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt
    30481 ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg
    30541 ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc catttacctc
    30601 aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa atctccatca
    30661 tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt
    30721 agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat ctactttgct
    30781 gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga atggaaaaaa
    30841 tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc
    30901 taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg ctatgaaacg
    30961 aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga
    31021 ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa tcggtttgct
    31081 gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc gtccagaact
    31141 gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa cggtgatgga
    31201 attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca ctcatgacat
    31261 ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt
    31321 agatatccag caagaggaaa aacagcagct cacagtgccc caattattag cgttgttcaa
    31381 acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat aatcagaaag
    31441 aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt ctttcgtttt
    31501 tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct tcttcctgat
    31561 agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac ttcaattatt
    31621 gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg aagtttcccg
    31681 gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg
    31741 gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa tccagcaaga
    31801 gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg aatctacgat
    31861 gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat ttgttgctga
    31921 tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac ctggacgagg
    31981 aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc ctcaatatac
    32041 gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc
    32101 gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga ttttatctga
    32161 agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga tcgggcttaa
    32221 tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca tctctgtctt
    32281 tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt
    32341 gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa aatcattcgt
    32401 attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga
    32461 aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc
    32521 tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca caaaaggaca
    32581 cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc cttattctat
    32641 cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta taaagcatgg
    32701 tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt acacgatcaa
    32761 aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt aacgataaaa
    32821 acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt gccttaatct
    32881 cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc agcatcatca
    32941 ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc gtcaattact
    33001 tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc agcagctcct
    33061 cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc ttgttccaga
    33121 ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct ttcccagtct
    33181 tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc taattctcga
    33241 gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc ttccactgga
    33301 aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc
    33361 cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc tttttattta
    33421 tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca cacccttatt
    33481 ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa aagatagtgt
    33541 cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt ctttaaataa
    33601 acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa taggaaaatg
    33661 tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt cctagttgct
    33721 ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata cattcctaaa
    33781 gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg aattgctagt
    33841 caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga cggaaatata
    33901 gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga aggtttattg
    33961 aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc aggcatacct
    34021 acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt agcacttcaa
    34081 gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga tgatttttat
    34141 aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa attgaatgta
    34201 gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc agattttcca
    34261 gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt tttattggta
    34321 aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa tccaaatgat
    34381 caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca gtcaccaatc
    34441 aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa tatctcttgg
    34501 aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg aacaatcctt
    34561 gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat aggaacaaat
    34621 gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat cagcgacaat
    34681 ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag tgatagctca
    34741 gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga tagcacggat
    34801 acatcagctt caagcgacag tacagatact acctctacca caagtgatag cacggacaca
    34861 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgacagca
//
LOCUS       DACDIP010000022        34180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDIP010000022 DACDIP010000000
VERSION     DACDIP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.022"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(148..2301)
                     /locus_tag="IV257_001265"
     CDS             complement(148..2301)
                     /locus_tag="IV257_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8814408.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2696..3544)
                     /locus_tag="IV257_001266"
     CDS             complement(2696..3544)
                     /locus_tag="IV257_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8814409.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3768..5708)
                     /gene="thrS"
                     /locus_tag="IV257_001267"
     CDS             complement(3768..5708)
                     /gene="thrS"
                     /locus_tag="IV257_001267"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8814410.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(6045..6224)
                     /locus_tag="IV257_001268"
     CDS             complement(6045..6224)
                     /locus_tag="IV257_001268"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8814411.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6271..8094)
                     /gene="cadA"
                     /locus_tag="IV257_001269"
     CDS             complement(6271..8094)
                     /gene="cadA"
                     /locus_tag="IV257_001269"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8814412.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8372..10651
                     /locus_tag="IV257_001270"
     CDS             8372..10651
                     /locus_tag="IV257_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8814413.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10744..11391)
                     /locus_tag="IV257_001271"
     CDS             complement(10744..11391)
                     /locus_tag="IV257_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814414.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11570..12289)
                     /locus_tag="IV257_001272"
     CDS             complement(11570..12289)
                     /locus_tag="IV257_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8814415.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12420..13571)
                     /locus_tag="IV257_001273"
     CDS             complement(12420..13571)
                     /locus_tag="IV257_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8814416.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13788..14528)
                     /locus_tag="IV257_001274"
     CDS             complement(13788..14528)
                     /locus_tag="IV257_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8814417.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14532..14855)
                     /gene="rsfS"
                     /locus_tag="IV257_001275"
     CDS             complement(14532..14855)
                     /gene="rsfS"
                     /locus_tag="IV257_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8814418.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14912..15505)
                     /locus_tag="IV257_001276"
     CDS             complement(14912..15505)
                     /locus_tag="IV257_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8814419.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15489..16136)
                     /locus_tag="IV257_001277"
     CDS             complement(15489..16136)
                     /locus_tag="IV257_001277"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8814420.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16149..16460)
                     /gene="yhbY"
                     /locus_tag="IV257_001278"
     CDS             complement(16149..16460)
                     /gene="yhbY"
                     /locus_tag="IV257_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8814421.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16476..17585)
                     /gene="yqeH"
                     /locus_tag="IV257_001279"
     CDS             complement(16476..17585)
                     /gene="yqeH"
                     /locus_tag="IV257_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8814422.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17582..18109)
                     /locus_tag="IV257_001280"
     CDS             complement(17582..18109)
                     /locus_tag="IV257_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8814423.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18289..19074)
                     /locus_tag="IV257_001281"
     CDS             complement(18289..19074)
                     /locus_tag="IV257_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8814424.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(19067..19936)
                     /locus_tag="IV257_001282"
     CDS             complement(19067..19936)
                     /locus_tag="IV257_001282"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8814425.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19937..21316)
                     /locus_tag="IV257_001283"
     CDS             complement(19937..21316)
                     /locus_tag="IV257_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8814426.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21324..21743)
                     /gene="fabZ"
                     /locus_tag="IV257_001284"
     CDS             complement(21324..21743)
                     /gene="fabZ"
                     /locus_tag="IV257_001284"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8814427.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21768..22244)
                     /gene="accB"
                     /locus_tag="IV257_001285"
     CDS             complement(21768..22244)
                     /gene="accB"
                     /locus_tag="IV257_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8814428.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22247..23482)
                     /gene="fabF"
                     /locus_tag="IV257_001286"
     CDS             complement(22247..23482)
                     /gene="fabF"
                     /locus_tag="IV257_001286"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8814429.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23501..24238)
                     /gene="fabG"
                     /locus_tag="IV257_001287"
     CDS             complement(23501..24238)
                     /gene="fabG"
                     /locus_tag="IV257_001287"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8814430.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24250..25167)
                     /gene="fabD"
                     /locus_tag="IV257_001288"
     CDS             complement(24250..25167)
                     /gene="fabD"
                     /locus_tag="IV257_001288"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8814431.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25274..25498)
                     /locus_tag="IV257_001289"
     CDS             complement(25274..25498)
                     /locus_tag="IV257_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8814432.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25551..26513)
                     /locus_tag="IV257_001290"
     CDS             complement(25551..26513)
                     /locus_tag="IV257_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8814433.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26523..26963)
                     /locus_tag="IV257_001291"
     CDS             complement(26523..26963)
                     /locus_tag="IV257_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8814434.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27269..28234
                     /locus_tag="IV257_001292"
     CDS             27269..28234
                     /locus_tag="IV257_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8814435.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28291..28521)
                     /locus_tag="IV257_001293"
     CDS             complement(28291..28521)
                     /locus_tag="IV257_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8814436.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28607..29494)
                     /locus_tag="IV257_001294"
     CDS             complement(28607..29494)
                     /locus_tag="IV257_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8814437.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29583..30806)
                     /locus_tag="IV257_001295"
     CDS             complement(29583..30806)
                     /locus_tag="IV257_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8814438.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30823..31902)
                     /locus_tag="IV257_001296"
     CDS             complement(30823..31902)
                     /locus_tag="IV257_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8814439.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(32033..33760)
                     /gene="ptsP"
                     /locus_tag="IV257_001297"
     CDS             complement(32033..33760)
                     /gene="ptsP"
                     /locus_tag="IV257_001297"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8814440.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33760..34026)
                     /locus_tag="IV257_001298"
     CDS             complement(33760..34026)
                     /locus_tag="IV257_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8814441.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
BASE COUNT         9418 a         7487 c         5875 g        11400 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa acaagaagta tttaatattt ttttcataaa atctttcatc
     2401 atttcctatc cttccgccta tttacattca tgcttcattg tacccaaaaa cttcttaata
     2461 aaaaagaaaa aatgggcgga gcaagaaatt ctttacctaa tttcaagata aaatcaactt
     2521 tttgtttcac agcaatcact taaagttaca tatccaacta tttttttcat taaaaaataa
     2581 acttattttt gaaaattatg ttttcttaag tcgtgaaaca ctttgatgga tcatttattt
     2641 gtggaaaact acgttttgtt attctaaaaa acttatataa aaaatagtaa ggtttttatt
     2701 tcgaatccat aaaatattct tggattgctt ctcgtgtttt tttcactttt tcgctaagct
     2761 tttcgttttt agccatatag aaataaaaaa tgatatcgat cgtagcaaat tgggccaaaa
     2821 gtgaattagt tgccgcactt cgattcgtga tttcttttgg tctagatgtt tgtacgagta
     2881 catcggctat cttgctaagc gggttatttc caaattgcgt caacgcaatg atttcgatat
     2941 tcatgctttt cgccagctcg gctaatgcga ctacatctgc acttctacct gagtttgaaa
     3001 caagccaaag cactttcttt ttctcattgc tgacaagttg cgggagcaag acgtggatat
     3061 ctttttcaaa aatgattggc ttaccgacac gagaccattt ttgcagaata tcttctacta
     3121 ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc tgccgattcc aaacactcta
     3181 cgactttttg gatcgtttct tcttctagaa tatctactgt ttcttttatc gctacttgtg
     3241 cattgttacc aagtttgttt ttgatagagt aaaaggactc atttgcttct atatcactaa
     3301 aaccttcatc gattccagtc tgtagttctg ttgaaatcgt gattttgaag tccgtcagac
     3361 tttgaaaacc gatttttttg gaaaaacgta caactgtcgg cgcactcaac ccggaagctt
     3421 ctgctaattc attagctgtc attgtaggga ccttctcaat attaccgata atgtagttgg
     3481 ccaacttttt ttctgctgaa gaataatctt ccatcatact ttcaatacga ctaattacgc
     3541 tcataagttc ctccaccctt ttaatgatag tctatgtaag tataccatat cagaccctta
     3601 ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc gcccaacaga atatcttatc
     3661 ccatcaatcc cacagattct attgagcatc tgtgtctttg aggaatacct gttatctatt
     3721 gttcggattg ctttctatct actacttttt tacaggaaat ttacctatta gttttctctg
     3781 ctgtaatttt tgacttctgc ttccatagca tcaataaaga tattcaaatc ttctacagct
     3841 gtttctttac ttccataacg acgtacgttc actgttgcat cttccaattc cttgtctcca
     3901 acaactaatt ggtaagggat cttttgtgtt tgagaggcac gtatcttata gcccattttt
     3961 tcattacgat catcaacttc tacacgcaac cccttcattt gcaaacgttc tttgatttca
     4021 taagcataat cgccatgtgc atcaacagaa acagggatga ttgttgcttg tataggtgcc
     4081 aaccatgttg gaaaagcgcc tttgtatact tctgttaaat aagcgacaaa acgttccatt
     4141 gtagaaacaa ttccgcggtg gataactact ggacgatgcg tattttcccc gtcttctcca
     4201 acatatgtca aatcaaagcg ctctggcaat aagaagtcta attgtatagt tgataatgtt
     4261 tcttctgtac caagagctgt tttgacttga acatctagtt ttggtccgta aaaggctgcc
     4321 tcgccttctg cttcaaagta atctagttct aattcatcca tagcggcttt cagcattgct
     4381 tgggcttttt cccacattgc gtcatcatca aaatatttat ctgtattgtt tggatcgcga
     4441 tagctcaagc ggaaacgata atctgtaata ttgaagtcag catatactgc caccatcaat
     4501 tcaagcgtac gtttaaattc atctttgatt tgatcaggac gaacaaatgt atgaccatca
     4561 ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag cacctgattt ttcatagcga
     4621 tgcatcattc ctaattcagc gatacgaatt ggtaattcac gataagaatg gatcgtgttt
     4681 ttgtagacca tcatgtgatg agggcagttc attgggcgaa gaacaagcat ttcgccatct
     4741 cccatatcca ttggcgggaa catgtcttct tgataatgat cccagtgtcc agatgttttg
     4801 tacaactcaa catctcccat tattggtgta tatacatgtt ggtaacctag actaacttct
     4861 ttgtctacga tataacgttc gatcgtgcgt cggattgttg ccccttttgg aagccagaat
     4921 ggcaaaccag aacctacttc tggggaaacc atgaacaagt caagttcttt tcctagttta
     4981 cgatggtctc tttcttttgc ttcttcacgg agacggatat attctttcaa agctttcttg
     5041 tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat tgtttgagtt tccacgccaa
     5101 taagcacctg ccactgataa taatttaaat acttgtatac ggccagtgga tggaacatgt
     5161 ggtccacgac acaaatcgat gaattcgcct tgttgataag cagtaatcac ttcttcttct
     5221 ggcaattcac taatcagttc tactttgtac ggatcacttg caaaaatttc taatgcttcc
     5281 tgttttgaaa gaacacggcg ttcgatcggc agattttctt tgacgatttt catcatttct
     5341 gcttcgattg ccggcaaatc ttctgcagtt acttggtttt gtccattatc tgtatcgtag
     5401 taaaaccctg aatcgatagc tggtccaacg ccaaagtgga tatttggata gaggcgacgc
     5461 atggcttggg ccatcaaatg agctgctgaa tgacggacta agccaagtgc atcttcatgg
     5521 tctggtgtga tgatctcaat acttgcatct tcctcgattg gacgagtcaa atcaacgagt
     5581 tttccgttca ctttaccagc taaagctttt tttgctaagc tttttgaaat gctttcagca
     5641 attgccaatg ttgttgttcc agactcaaat tctttgactg caccatctgg aaaagtaatt
     5701 tttatcataa tagtttcctc cttgaaatgt tttgattgat cagatcatca agctgaaaaa
     5761 caaaaaaatc ctagcatctc ttgaaaagag atactaggac gaaaagttcg tggttccacc
     5821 taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg gtccgaccgc ccttgcttca
     5881 acaagggttt cgaaagtggt catcctttgg ttttctcctg gaaaagtttc agcctagctt
     5941 ttcctctctt atcgaagact ctcaaattca gttggctttc tcatcaatca attatgtgtt
     6001 tatttaacca ctaataaata gaaaaatcaa gtgggaaact aaacttattt tttccattcc
     6061 ccatttacac cgtatgttcc ttttttcatc tcattgatga tgacatggat gtgttctttt
     6121 ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt tcatcatatt ttctaattgt
     6181 tccggactgc gaccttctac taattcgaca tgtacgaatg gcataatgct ctctcctctt
     6241 tctgatcgtt cttttttcaa ctcgattcta tcatactatg tttgtcttgg gtactgaatc
     6301 aggaaaaatg gctggatctt tttttgctct caaggcactc aaaatagaga ccgtatccac
     6361 gacttcttgc aaaacagctc cgaataatgc ggggattagt cccgtacttg caattaacat
     6421 caaaaaaata caaataaaga tgccaatcag tacagactgt ttcgctattc tcatcgtatc
     6481 tcttgaaatc actactgctt ttgctacacg caagagatca tctttcaaga tgaccgcatc
     6541 tgcactttca ctagcagctg tagaaccatg agcccccatt gcaataccga tatctgcagc
     6601 agcaagtgct ggtgcgtcat tcacgccgtc tcctaccatc gcaacaggtc tttgatcttt
     6661 agggatactt tctaaaatgg tcagtttatc ttgaggcaag ctacgggcat gtacagaatc
     6721 taattggact ttttctgcaa tcgtttgagc aatggcagat ccatccccgg tgatcatcat
     6781 caaatttttc acatcaagtt ttcttagctc cttgattgtc tttgctgctt ctgggcgaat
     6841 ctgatccgaa aataggatcg ttcctgcaaa agatcgatca attgttacat agattgctgt
     6901 attggacgtt tctatttctt cagctgaaac aaaagaggca cgtccaatct ttacttcatg
     6961 attattgact actgcttgga ttccttgacc ggtcacttct ttcagttgac taacaggata
     7021 cagttggctg tttttcacgt tgttcactaa cgaacgagct aagatatggc tagattcctg
     7081 ttctgcacta gctgcataca gaagtagctc ttcttttttt attccattga ctggctgaat
     7141 cgcctctact tccaatatcc ctttggtaag cgttcctgtt ttatcaaaag caatcgtttt
     7201 tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca acgatcccgt ttttacttga
     7261 acggctcatt cctgcaacta aagcaatggg cgcagctaga atcagtggac aaggtgatgc
     7321 cactaccagt acctcagcaa atcgtaaagg gtcttttgag aaccaccaag aaataccacc
     7381 gatcaaataa gcaatcaacg tgaaagggac tgcataacga tcagctaaac ggacaaatcg
     7441 ggcaggcttt tcttttgatt cttcgactag tttaataatc atttgatact ggctgtctgc
     7501 ggcacgtttt tcagcgatca tttggatcga tccgtctcca tttaaagacc ctgacataat
     7561 ctgatctcca ggtttcttgg tttccgggcg ggactcacct gtcaaagaag attcatcgac
     7621 cgaagattct cctttggtca ctcgaccatc tgcgggtaca agttcgccag gcttgactag
     7681 taactggtca tcaatattta tttcatcgat tactatatcc tgcaattttc cttctttgaa
     7741 tcgatgggca attcttgggg tgttatctaa caaagactgc agttctttac ccgctcgttt
     7801 attcgcgtaa tcctctagac tatctccgcc cgtaagcatg atcaatatca tcaaactagc
     7861 ccagtattct ttgacggcta atgttgcaag gattgcggta atcgctaata catctacgcc
     7921 atatcttcct gaacgtaacg tcttgatcat ttccaaaaac atcaagaagg cgagaatccc
     7981 tccagtaaat gcgacaatcg caaatgcaag ggccggctgg tgaaaaacaa attcacttaa
     8041 taaagctatc ccaccaataa gcaaagtaag taaaaatttt ttgaaatgtg tcattgtttt
     8101 ttctcctctt ttatgtgact tagagaggtt agtaataaat ttccagctgc ctctttcatc
     8161 ttaagcatag tccttgacta gcacttttta aaacaatagg ccttaattga aaacaactat
     8221 cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa aaatattcag caaggattac
     8281 tttttcgatt catcagttaa aaagtacctt ttttcgaaaa actatgctat acttttaatt
     8341 cgttatttac ggcggaagga gcaaacatgc gatgaatgag aaacaactag aacaaacgca
     8401 cgtggatgaa acgatcgaac taatccagat cgaacaaaat atgctggata caaaacaaca
     8461 agaattgact caggaaatga atcattcgac aaaagacaca gctaatcaca agattcgagc
     8521 tggttccaat gaagcttttt atgaatctgc tgttgaatat cgccaacatg agcaggaatt
     8581 gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc ctgcaaacgt tgtctgtcat
     8641 gaaagatagt ccttattttg cccgtatcga ctttaaagaa gaccgtgaaa cagaaacatt
     8701 atatctaggt atcgcttctt taagagatct ggcagaagaa acaatcgtta ttgactggcg
     8761 cgcaccaatt gccaatcttt attatgaagg tgagattggt cccgcatttt atgaaactga
     8821 aacagataga atagatgtcg atctgcagtt gaaacgacaa tttaaaatcc aagaaggacg
     8881 tattttatct atggtagata cgtcggaagt aatcaatgat gactttttgc tagagatatt
     8941 ggatgaatca tcaagcaacc atatgaaaaa catcgtctct acgatacaaa aagcacaaaa
     9001 tgcaatcatt cgggacacaa caagtaaagt aatgctgatt gaaggaatcg ccggaagtgg
     9061 aaagacatcc gctttgcttc aacgtattgc ttttctgttg tatcataatc gcaagtggct
     9121 cgaggctgaa aacgttcttc tattttcacc caaccatttg ttttctgatt acatctcgac
     9181 tgtattgcct tcattaggtg aaagcggcgt gcctacacag acattcaaaa attatttggt
     9241 gcagctgctg ccagagtttt ctttactaga tgaacagcaa caagaaagtg gttttttgct
     9301 aggggaaaaa gatccgatac aagtgatgaa aagcggattg accttagtcg atcagattga
     9361 tcgctatatc caaggcatca caaactacgg tcccttgttc cgtgatatga aaataaatgg
     9421 ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat aaagaaacaa atgaacaatt
     9481 acctttacat catagacttt ctctattaca aacgaagctc ctgaaaaaat taggcggtct
     9541 gcaaaaagac gaaacacgtc agcaatgggt caaagatctg gcggaagaac agctccaaga
     9601 actatacgca acagatccaa atcttgaata tacagaacaa aaggagcgtt cattacgtaa
     9661 aaaactggcc aaccaaatcg tacgtaaaag attccgtcga atccatcgag ggatcatgaa
     9721 ttatcaattt gtcaatataa cgaaacaata tctccacttt ctacaaactg tgccactttc
     9781 tcttctaaca cgacataaaa tcagcgagac caggtggcag gaacatttga caactgttcg
     9841 tacacacttg cgtcaaagag aattgcgtca agaggatgct gtactattct ttttactgat
     9901 gcgccgaaca tatccagtga cggttacaca caaagctcgc tatatcttta ttgatgaaat
     9961 gcaggatttc gcaccatcac aagtggcatt gctgcaagcc ttgttcccaa aagcaaattt
    10021 gactttctgt ggagatctaa atcaaaatgt ctttggaaat gaaacgatcg ttggatcatt
    10081 ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa ttaacgacaa gctatcgctc
    10141 cacaaaagaa atcactgatt ttgctaatca ttttattgtg aaagaacatc aagtagaaac
    10201 aacagcaaga aaaggagacc ttcctttgct catccaagga acaacagatg atatcaaatt
    10261 gaattggtta gcagatacca ttcgaacgac tcagggaaaa gcccggtatt ggcgtacagc
    10321 aatcatagct aaaacacaag aagaatgcga agctctttat gctcaactgc cagacgagtt
    10381 gaaacaacaa gtccagctga ttttaaatga gagtgacttt atgaagcgtt ccattatttt
    10441 acttccagcc tttttagcaa aaggtctgga gtttgaccga gttttcttgt gggatgtaga
    10501 tgcagaacat ttttcttctc agcaagacaa attgatcctt tacaccatgt gtacacgtgc
    10561 gatgcatgaa ttgacccttt ttacctccaa taatgaaagt cctttgttaa acaatatgga
    10621 tatttcatct tatgagaaaa aagtactgta agtaaaaaga caactgctat cattaatgtt
    10681 agcagttgtc tttttattta caataataat tgcagaaaaa gtaattcaat catttaaggt
    10741 catttattct atatataaat ccaaatctgt ttcaggatat tttaatttga caagcgaaat
    10801 aatcattttc agttcttcca tgttttctgg atattctggg tcaaatacct tgctatgacg
    10861 gccaatcccg ccattgtcga tcaatcggta aattcgattc cattcaatga attggtcaat
    10921 tgattggaaa ttgctgattg gatagccgcc acaatccaaa atctctgata cacgaaatgc
    10981 taggtatgca ctatcgatca ctttattttt tttcgtgtct atcttctcat ttctttcgat
    11041 aagaattttt tcaggacgat gtttcaagct ccactctaat agtagaaaga aagcctccat
    11101 ccgatactct tcttcaaata accgaaaggc tgaatcagaa cgacgaaaaa gcagcggaat
    11161 cagataattt tgtctataaa tcagcattgg tgcgcggtcc accatacact ctaaataacg
    11221 gttcagatac cctgtaccga catagtttcc taatcgatag ccttcaacgc cgaatcttgc
    11281 gtatttttgc gctaattttg aacaatcagt tacgttcaca gctaactctt ctactttgcg
    11341 gtaaccgctt aagatacgtt caaaaggttc taaagtgatt tttgcttcca tattcttccc
    11401 cgtccatctt ccacatactt catatcattt cacatgatac ttatgctctc atcataccac
    11461 aggttgaatt agaaaagaaa ataaatcaca caaaaaagca agacaatctg ttagtgagat
    11521 tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa actggttgct tagtcttcta
    11581 atgccacatt atggaacaca tgctgtacat cttctagatc ttccagtgca tcaatcattt
    11641 tttcaaatgt ttcttgatct tctggagata gtgttacctc gttttgaggg atcatttgga
    11701 tctcagcgat tgaaaaatca gtgattcctt tttcttttaa tgcttcttgt atactatgga
    11761 aatccgctac ttcaccgtaa acgatgattt gtccttcttc ttccattaca tcgcgaacat
    11821 cgatgtcttt ttccattagg tattcaagga tctcgtcagc gtcttctcct gcaaaagcaa
    11881 agattgctgt attgtcaaac atgtaagaaa ccgctccgga aacacccatg ttcccgccat
    11941 ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt cacattgttc gtcaatgtat
    12001 caacaatcac cattgagccg cttggtccaa atccttcata gcgcaattca gagtatgttt
    12061 catctcctgc accttttgct ttttcgattg ctcggtcaat aatatgtttt ggtacattat
    12121 aagttttagc gcgatcaatg acgaaacgta gtttttggtt tgcatgcgga tcaggatcac
    12181 ctgattttgc tgccgcgtag atttcaatcc caaattttgc ataaatccgg ctattattag
    12241 catctttagc agttttcttt gctacgatat ttgcccattt acgtcccata ttttcacttc
    12301 gctttctgat ttgatagttt cataacgcaa aaacgtcctg ttccattata cttgtgaatc
    12361 acgattctgt gaagcttttt ttctttcttc cttcattctt ctcattttta gtcaattatc
    12421 tatactcgga ttgggatttg cccgaaattc tgttccggaa tattgcctcc caattgatag
    12481 atttgatcac ttctaactaa cagttccgct tcttgtgcct ctttttttcc gattcgggaa
    12541 taaatagaaa tctctttttt ttcttttaag aatcttcttc cagcatcggt aaatccaagt
    12601 agtcgaagat aatttttatt ttgttggaca ggaatctctt gctttttgat attcagcaag
    12661 acataacaag ataatcgttg gatccgtgtc catgtatatc tttttgtttt tacctttgac
    12721 acaaaatcct caaaactatc agcagttgca gctgctgttt tcatgcgcga ttctattcct
    12781 tcggtcatct gatagatatc tgtcagttcc agcaaagtag aagataacaa ccgataacgc
    12841 aaatacggcc aataagattc ccaagtctgc agcgaacaag ttgaaagatc ttcttctgtc
    12901 tgaggcggca caagtccagc tattcttttt ccttcttcta acgcatggcg aatggctgtt
    12961 gcactggcta tcctctgatc tgtgatgatt ttttcatgat agcctgcact tttacgtcgg
    13021 atcggatgta attccatagg gtggacatac ttggcatttt cctttgcata acttaatccc
    13081 aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg gatacagact gcgaaaaacc
    13141 tcagtcattt tttgcggata actatacgtt tgatcagaca gttcttgaaa tgcctcttct
    13201 atcctatctt gattctcttg gacaaagcgt ccgaaactgc tgtaatcaaa cgaagtctct
    13261 tcttctgttc caaaacacaa actgtcacag tccagtgttt gcaagatttt tactgcacct
    13321 ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg gaagctccac tactaaatcc
    13381 acaccatttt gtaaagcttc attcgcgcga tgccatttat cgatgatagc cggttcgcct
    13441 ctttgcaaaa aattcccgct cattacagca atgacgacct ctgctcctgt tatttttcgt
    13501 gccatttcca catgatagcg atgtccattg tgaaaaggat tgtactctac gataatcccg
    13561 cagcttttca tgcgcatcct ccttcatagt tatcgataaa aaagaaatag agacaagtaa
    13621 aaaaaggcaa aaatcggaaa aaatacccga actatataaa ctattctcgc aaacacaata
    13681 cttctgcgac gaaaagctgc actgcaagag caatgcctat atcgttcgga tatataattt
    13741 atgttgaaga tttattcttt tcttgttaac tcgagcaggc aaaaccgcta gtctttgtat
    13801 gctacaaaga accagcgctt tgatttctct gatgctgctt cgtctgtaaa atcagaaaat
    13861 gcttcgactt tcgaaaagcc gctgttttcc agcattctta aataactttc caatggatag
    13921 gtacgctctt gatgtagttc atcttcacga ataaatcgac cagacccttc ttgttcttca
    13981 acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc ttcctggata actatcccac
    14041 aaaaaagcaa aattatcccc ttgataatga tagctatatt ctggaaaaac ctcatctact
    14101 tgataggtgg agtgtacgtc aaataaaaat cggccatttt cttctaacgc ttgatagact
    14161 tcatcaaaca cttgctgtac ctcttgtcta tttttcatat agcacagcga atcagaaaaa
    14221 caggtaattg cctgatattg tccgatttct gataaatcga gcatattgcc ttccacaaat
    14281 tggatcgatg cgtcttcttc atatgctcgc tggctggcaa tcatcaacat ctcttctgaa
    14341 aggtctaacc ctgtcacttt atatccatct tttgcaaagg caaccgctaa tgctccagta
    14401 ccgcatgcca gttccaaaat attttttgtg tcttcttgta aatgacgttt cgaaaaagct
    14461 aaccattgct gataaagtgt ttcgtccatc acttcatcgt aaatgaaagc aaatgtttca
    14521 taagccatat gttaatccaa ccattcacta agatcgacta gcggagcatc tgaccatagt
    14581 ttttccagat tatagaaatt tcgttctggc gcatggaaaa catgtacgat cacatctcca
    14641 agatcgatca atacccattt tccgccttct tttccttcaa tacgttttac ttcatatttg
    14701 ttttcttctt ctttatcaat gatttcttct gtaattgcgt tgatttgacg ttcgctattt
    14761 gctgaacaga tcatgaaata atctgccaac aaggaaacat ttcgaacgtc taaagcgata
    14821 atgtcttctg cccgttttga atctgctgct tcaatcgcaa tttttaattg ttctttacta
    14881 tctatgatga tttcctccta atatgagcag cttatttttt tgctacccag taattatatg
    14941 tttcgattgt ttttggataa atctgttgtt cttgcgcaag caaatgggcc aatgtatgtt
    15001 ttgtttcaaa tgctacagct tcgtctagat cagcccatgc aatggctcgt gcatcttgta
    15061 ccccaggaaa atctcgacct ggttcaatat agtctgctac ataaatgatt ttgtcaagga
    15121 gactcatctt agcagcccca gtcgtatgca cccggattgc atgaagaatc tctgcatcgg
    15181 tgattcctaa ttcccgctct acaaacgaag cgccaacaag accatgccag attgcattat
    15241 tataattcaa taactccgga tcaaaaccgt ctcttacgat taccattttg aactcctcat
    15301 ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat actagctttt tcaggagaag
    15361 caccgtattt tttggcaaga gccacggctg tttcttctac tccaagcaca tgcttgaagc
    15421 gttgttcgct cattctcatc tgtatttttt gcatcaggtt ttctctagaa taagcagtat
    15481 attcattact atagtccata ttcatacaac ccttcatttt gaatataatc aattacttta
    15541 tctggaacaa ggtagcggat agaacagcct tctttgatct tttgacgtat tttcgtggaa
    15601 ctgatgtcga tctcaggtac atctacccaa attacaggat atggagtatc cgttgtgtat
    15661 cctggtcgac gtatacccac aaagttgacc atagaagtca gctcatcgat tttataccat
    15721 tttggcaaat attctaccat atccccaccg ataataaagt aataatctgt atccggattg
    15781 ttttgcgtca attccttcat agtatcatag gtgtaacttt tcccaccacg agctaattca
    15841 atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta attccaacat gttgagtcga
    15901 tgttttgcat caatcgtctg tttctcatca acatgaggtg gctgataaga aggcatcaaa
    15961 aatacgcggt caagacctaa attccggcca gcttgttcag ccatcactaa atgtgcaagg
    16021 tgaacgggat tgaatgttcc gcccaagatc cctacttgct ttttcttctc taagaattga
    16081 ggcatttctt caggaaataa ttgtgaacga acaaatgtct tagcttgcgt attcatcatg
    16141 gtcactcctt atattgcttt tacttctttt gatagccttt gatacttttc ttttgtggaa
    16201 ggtttgtata atacaataac acgtccaatg atttggacga tttcgcaatc tatttctttt
    16261 tctaatactt gagctgcttc ttctgcaatc tcatctgtat tttgaagtaa cgatactttg
    16321 atcagttctc ttttttctaa agcttcttca atttgaacga tcattgctga attgatccca
    16381 ccttttccga tttgaaaaat cggctgaaga tgatgcgctt tgctacgtaa aaaacgcttt
    16441 tgttttcctc ttaaactcat aattttcctt cttccttaaa tcaatgcttt gcgtttcaat
    16501 acatctacac cctttggcgc ccagcccgca attactactg gcgctgttac agtgatccag
    16561 cctagaccgg caaaaacgat gtctgttttt tctttcacag aaaactcaaa acgaacaagc
    16621 tccggaaatg cctcgacttc atcaggacga ggaggctgta ataatccacc tacatgtttt
    16681 tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa tatttacatc atttgacaca
    16741 taagcaataa atgaagcacg attgccgctg ataaaatcaa atcttgcaag tccgcccaag
    16801 aaaagtgttt gcccttcatt cagctgataa actttaggtt tgatttcttt taacggtgca
    16861 atgatccgca aatctttttt acctaagtag tgcgccattt gatggcgatg gataattccc
    16921 ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct cgattttatc caaggtcgtt
    16981 cctggaaact gcgaagttgt gattacatct tgtactcccg ctgtattttt gatgatttga
    17041 ttgataagcg tcgattttcc gacatttgtt actccgacga tatagacatc ccgtccatcg
    17101 cggtattctt cgattttttc taatagttcc gtcatctcat gagatttctt agcactagtt
    17161 aataatacat ctactggacg aattcctgct tcgtaagcac gttccttcat ccattgtgtt
    17221 aatttagttt ttttcaatga tttaggtaat atatccactt tgttcccaac taaaagtact
    17281 ggattgtcac cgacaaaacg gtgtagtcct gggattagtg aaccattgaa gtcaaaaata
    17341 tccaccacat tgacgatcaa tgcatctttt tgccctaatt cattcagtaa gcgcaaaaag
    17401 tcgtcatccg tcaatgagac atcttggatc tcattataat ggcgtaatct gaaacaacgc
    17461 tgacaataga cttccccgga agctaaacct ttttccaaag ccgtttgcgg cgtgtatcct
    17521 aattcaccgg gtttgtttgt ttgtataacg gcaccacagc cgatacattg taattcttct
    17581 gtcattcgat tccacctcgc cagcccatat cagggtgttt tgctaataga taggccataa
    17641 ttcttttttc ccaaaaacga ttaaattgtg tcttccaact atctgttgcg ataatgggtt
    17701 ttacaaggat gctacgtatc cctgcacgat tggctccacg tatatcagtc ataatctgat
    17761 cgcctaccat tactatctca tcatctgcta aatccaattg tttctttgct atgttgatcc
    17821 ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag atcaaatttt tcaatcgctc
    17881 gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac agggattcct gcatttttca
    17941 tttctaaaat ccaagtaagc aactcttctg ttccatctgg attattccat gcaatcaacg
    18001 tattgtctaa atcggtcaat actgctttga tgcctagatt ctttaattgg gcaggtgtaa
    18061 tctgatagat ggcttctacc atccatgttg gtttgtaatt tgaaaacatg ctgtctcctc
    18121 atctgcaaaa aatgaaggag cacataaagt actcctcaac cccgtattat actgcatatc
    18181 agcaaaaaaa actacctcta tactcgttcg tctttcagaa aaagcatttc tttacaaaat
    18241 gaccaaaaat aaagattgat cacagaaaaa tttagaaaca aaaaacaact aatattggcg
    18301 gaacctcttg tatcgttttt ccagtaattt atgctgatct aatcgaacaa gttccgtcaa
    18361 tctttcaatc aacgcttttt tcataatgtg tgcgacttta tatcgtggca actgctcgcc
    18421 gttgaacgtt tcaggaataa ctcgatcaac gattcctaac tcgcgtagtt ctggcgcagt
    18481 aattttcatc aactcagctg cttctgcggc gcgactaccg tctttccaca aaatcgacgc
    18541 aaatccttct ggagaaagca cagcataaat cgtttcttcc agcatccaga cttcatccgc
    18601 taaacctaga gcaagggcac cgccacttcc accttcacca atgatgatgg cgatgatcgg
    18661 tacctttaaa tcagacattt cgagcagatt tctggcaata gcctctcctt cgccgcgctc
    18721 ttctgctcct acaccgcaat atgcacctgc tgtattgatc aaggtaatca ccggtctatt
    18781 gaacttttcc gcttgtttca tcagtcgtaa agcttttcga tagccttcag gatggggaga
    18841 accaaaattt ctgcggatat tttcctgcag atcccgtcct ttttgaatac cgatcactgt
    18901 taccggtttg ccatctaatc gagcgattcc accaacaatc gcttcgtcat ctgcaaataa
    18961 acgatcacca tggcattcct cgaaagaatc gaagatctct tcaaaaaaat ccaagcttgt
    19021 ccatcgttct ttagctcttg ctaactgaac gacatcatgt gctgtcttat ccacgatcta
    19081 tccatccttt ctgtgtatgc aattcaagaa gcgtatggat tttctgtttc aattccatgc
    19141 ggggaacaat ctgatcgaca aaaccatgag ataataagaa ttcagccttt tggaaatctt
    19201 ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct tccagcaaaa ccgatcaagc
    19261 tttggggttc tgctagaata atatcgcctt ccattgcaaa actggcagtt actcctccgg
    19321 tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc attgctatgc cgtttgacag
    19381 cagcagagat ttttgccatc tgcattagag aaaagattcc ttcttgcatc cgtgcgccac
    19441 ctgaagctgt gaacaagacg actggaagac tttcctttgt tgctcgttcg aataatctgg
    19501 tgatcttttc tccgacaatc gttcccatac ttcccatgat aaaattagga tccatcactc
    19561 caataatgaa gggaatcccc tgaatggttg ctttaccagt gaggactgct tcatgaagtc
    19621 ctgttttatc ttgcattttt ttaatttttt ccgtatattc gggaaatgct agcggatctt
    19681 tggttacaag atccgtatcc cattcttcaa aacttttttc atcaataatc aaagctagac
    19741 gctgccatgc accgattcga aaagcgtatc cgcaatgagg gcatactttt tcttcaccga
    19801 tgtctttcgt ataaaggata tgtttgcaat ttggacactt tgcccacatg ttatcaggaa
    19861 cagatggcga gttagcaggt ttgctctctc tattaggatt gatccgaata tatttctttt
    19921 tcttaaacag agccatctat gcgcctccta tctccgtttc tggtgtccaa ttcggtaaaa
    19981 attcttcttg taaaaatgct gtgctgtaat cacctgctat gactgcagga tgactaatca
    20041 aatccatctg aaattctgcg ttcgtgatga ttccttctgt gataaattca cttaaggctc
    20101 gctgcatctt cattaatgct tcaaaacgcg tatttccatg gacgattatt tttgcaatca
    20161 ttgaatcata atatggcggg atcgtatatc ccgggtaaac tgcactgtct actcgcagtc
    20221 ccatcccacc tgctggaagc agtaagtttt ggatcttccc tggagaagga gcaaaatgga
    20281 aagcaggatt ttctgcatta atccggcatt cgattgcgtg tccttggaat actacttctt
    20341 cttgacacac attcaaaggc tcaccggctg ctatctccac ctgttttttt actaaatcga
    20401 ttcctgtgac catttctgtt actggatgtt ccacttggat tcgagtattc atttccatga
    20461 aataaaaatc gccttcttca tccatcagaa actcgatcgt tccggcattt tcataattga
    20521 ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc tctttttgtt tgtgagatca
    20581 ctacagaagg agattcttcc agtacttttt gattgttgcg ttgtaaagaa caatctcgct
    20641 cgcctaaatg aatgacatga ccaaatcgat cacctaagat ctgaacttct atatggcgag
    20701 caggataaat gattttttcc aaatacatat catcatttcc aaatgccgct gctgcttctt
    20761 gctgagctga agaaaaatgc tgtgggagct cttcttttga ttgaactttc cgaatgcctt
    20821 tcccgccacc acctgcagct gctttcagca tcactggata accaattctg tctgctattt
    20881 ccaatgcttc ttctactgta gataatacac cagtacttcc tggaatgact gggacatttg
    20941 cttcttgcat caattgacgc gcattgatct tatttcccat tgcatcgatc gttgcacttt
    21001 taggaccaat aaatgtgata ttgcactctt cacacatgga ggcaaatcgg ctattttcag
    21061 ataaaaaacc aaatcctgga tgaattgctt cggcttttgt cacgattgca gcgctcagta
    21121 cttcctgaac attcagatat gaatcaacgg cttttgccgg gccaatgcag attgcttcat
    21181 ctgctaactg tgtatgaagt gcttcggcat ctgcttctga atatatcgct acggtttgga
    21241 tccctagttc gcggcatgca cgaatgatac gtacagcgat ttcaccgcgg ttagctatca
    21301 atactttaga aaacatattt cacctatcca atcataaagg ttaattccgc ttcggctact
    21361 tttttaccgt ctactttagc gattcctttg cctattccgg ctgaagcttt cactttcaat
    21421 atctcaactt caaggtataa ggtatctccc ggtgtaactt tcttacggaa ttttgctttg
    21481 tccaatccgc cgaaataagc cgtctttccc ttgaattgtt caagagaaag taatgcaact
    21541 gctcctgctt gagccattgc ttcaacaatc aatacccctg gcataacagg ttcttctgga
    21601 aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt tcttagcaac gatccgttct
    21661 ccttcaacca tttcttccac tcgatcaatc aaaagcattg gataacgatg tggaatgatt
    21721 tcttttattt cttgtatgtt cattgttttc cctctttcct gctttgctta tttttgctga
    21781 atcctaaaga gtggctgtcc atattcgaca actgactcgt tttctacaag gatttctgtg
    21841 atcactccgt cggcctcact agttatctca ttcatcagtt tcatcgcttc aataatgcag
    21901 ataacttctc ctttggcaac ttgatcacct acttttttga aagctggttt gtcgggcgct
    21961 ggttgcagat aaacgattcc cacgattggc gaaacaacct cgattccatc aaatactgcc
    22021 gattcttttt ctttcattat ttctggagca gttgttgctt gttcagatat gacattgact
    22081 ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat tcatgcctcg acttgtttta
    22141 tttttattca tatacaattc aaattgacct tcacgcaaat cgaattccgt tagcgaggat
    22201 tgatcaaatt gactgactaa ttctctgatt tcattgatat ccaaaattat tcctcccagc
    22261 gtttcatgca cagaaccgca ttatgtccgc caaaccctaa tgaattactc attgcataag
    22321 taaaacttgc aggtttgctt tcgtttttga caactgtcaa gtcaatctca ggatcttgtt
    22381 tttctacatt gatcgtaggt gggataaatt gatgttctaa tgccagcaaa gttgccaagg
    22441 cttcgatccc gccggctgct cccaataaat gtccagtcat cgatttcgtg ctgctgacgt
    22501 aagtatctct gaaattttca cctaatgcga tttggattgc ttttgcttct gcctggtcat
    22561 tggctggtgt acttgttccg tgagcattga tatatccgac ttgttccggc tgtacaccag
    22621 cttcttccat cgcaagcttc attgctttag atgcaccaga accatctgga gtaggagctg
    22681 tcatatggta agcgtcacag tttgcaccgt atccaacgat ttctccatag attttggcac
    22741 cgcgagcttg tgcatgttct aaagattcaa gaactagaac gcctgctcct tcacccatca
    22801 caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg attaggatca gttgattctg
    22861 agagtgctgt taaagctgca aatccagcaa tccccatctc acaaatcgtt gcttctgttc
    22921 ctccagcaag aatgacatca gaatagccat gtttgatgtt acggaatgct tccccaattg
    22981 aattcgttcc gctagcacaa gctgttacga tagctgtaca aatacctttt gcacctacac
    23041 gcaaagcaat atttccagct gccatgttgg caatcgccat tggtacgaac attggagaaa
    23101 ctcgttttgc acctttttca tgtagacgaa taacttggtt ttcgattgtt ggaagaccac
    23161 cgatcccaga tccgatgatg acgccaaacc ggtcttgatt cattttttct ttatctaaac
    23221 cgctcatctc cattgcttct aaagcagcgt agattccata gatcgaaaac atatccattc
    23281 gtttgctatc ttttttaata aaatatttat cgtaagggaa atctttgact tcacccgcta
    23341 aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg tgcgatgcca ttcgtcccct
    23401 cttttaagct attcaaaaag ctgtctgcgt catttccaat aggtgaagtg actccatagc
    23461 cagtaattac cactcgattc atttgtttgc tctccttttc ttatccgtgc atgaccaatc
    23521 cgccatcgac attgatgact tgtccggtga tatatggatt ttgtgctaag aaaactgctg
    23581 tttgtgcaat atcttctact tgtccgaaac gctgcatcgg aatttgcttt tccgcttgtt
    23641 ccttgacttt gtctgctaaa acttccgtca tatcagttgt gatgaatcct ggcgcaatgg
    23701 cattacatgt aatgcctcta gttgcagctt ctcgtgccac agattttgtt aatcctacta
    23761 cgccagcttt actagctgca taatttgctt gaccaatatt tccaatcaac ccagatacac
    23821 tagagagatt aatgatggcc ccttctcgct gcttcatcat ttttttcaat acgtgctgcg
    23881 tcatattgaa ggttccaatc aaattgatat ccagacattt tttgaaatct tctgcatcca
    23941 ttcgcatcac aagtttgtca tttgtgattc cggcattgtt tactaacaca tggatcgatc
    24001 caagcttttc ttctgcttct ttgatcattt gtcccgcttt ctcataatca gagatgtcac
    24061 cggaaactcc tacgcatttt acaccgaatg cttcgatttc ttctatcttt tcttttggaa
    24121 tctctccgcg accattcaaa atgatatttg ctccagcttt tgcaaaagct aaggccatcg
    24181 ctttgccgat tcctcttgta ctacctgtga taaatacatt ttttcctttt acatccataa
    24241 cgttcctcct tatagtattg ctattgtttc atcaaatgtc tgcttattct caacacgcag
    24301 aactggtatt gttttatcga tttttttcat aaatccgctt aatacttttc cggggccgac
    24361 ttcgatgact tgttcgatgc cgattgtttt taacttatgg atactctcat agaaacgaac
    24421 aggttgcatg acttgctgtt caagtagtcc cgcgattgtt tctttcttca ttatttccgt
    24481 tgttgtgttg cttatgatag gaaaagaagg ttcagagaat tgaatctgac ttaggtcctt
    24541 cgccagtttt tttgctgccg gttctaaaat agctgtatga aaaggcccgc tgacatttag
    24601 agggatcatt cttttgaacc ctttttcttt taatagtaaa acagcttcgt ctactgcttt
    24661 ttcttcgcca ccaatcacga tctgctgagg tgtattataa tttgcaggag atacgatgcc
    24721 gtacttgcta gcttcatgac agctttcctc gatcgtttca atcggcgcat tcatcactgc
    24781 aaccattttc ccgcttccag ctggggtagc ttctgtcata taagcgcctc tttttgctac
    24841 taacgcaact gcttcagtga aggatagcgc attactagct actaatgccg aatattctcc
    24901 taaactcagt cctaaagctg catctgggat gattccatgc tccttcaata agcgataata
    24961 cgcgatgctg actgttagaa tagctggctg tgtatattgc gtctgatcca aacgttcatt
    25021 ttctgtaaag caaagttcag ccatatcgta gccgagaatc tcacttgctt catcaaatgt
    25081 ctgctttaca acggcttctt catataaatc ttttcccata ccttgatatt gtgcaccttg
    25141 gccactaaat aagaatgctg ttttcatgtt tttctccaat atatccagaa tatatgtttg
    25201 attcttccta ttcaaaaagg ccgagacatt cctatttcag gaaatgtccc agcccccaac
    25261 ctaattttaa tgattatgca gtttgttttt ctacgtaagt cactaaatct tgaaccgttt
    25321 tgattccgtc ttctgtttcg attttcacgt caaattcatc ttcaatttca ttgatgattt
    25381 ggaataaatc caagctgtca gcttctaagt cttcttggat gtcagtagtc aatttgattt
    25441 cttcagtttc ttttcctaat tcttctgcga tgatttcttg aattttttcg aataccataa
    25501 taatatattc ctccgatatt gtttgctttt tatttttata atttaacagt ctacaactcc
    25561 atgagaatac ttccccatgt taaacctccg ccatagcctg taaatattac tttctgctgt
    25621 tttcctaagg tgatctttcc actagaaact gcttcatcca ataagatagg gatgcttgca
    25681 gcagatgtat tcccatattt atccatattg gtcaggaact tttcttgcgg catctttact
    25741 tttcgggcaa ttttctcaat aattctgagg tttgcctgat gcaaaagcag ataatcaact
    25801 ggtttgtctt tcatgacttc ttggatattg tttgcaacgt ctcgtacggc aaaatcaaaa
    25861 atatctctgc cagtcatttg caaataatac gaaccttctg aatcttctga atagaacgga
    25921 tttttatttt gctggtagcc agcggtcaat gagctacttc tggtaccgtc agctgctaat
    25981 ttttctttca gcaatttttt ctgtggattc gcttctaaga gaatcccacc tgctccatcg
    26041 ccaaacaaca cagcagtcgt acgatcagac caatcgatta gcttagatag tgtttcacca
    26101 ccaatcacca gtcccttagt tgagccgcag cggatcattt tttcagccat gcttaacgca
    26161 taaacaaatc ctgagcacgc agcactgata tcaaaagcga tagcatgagt tgctcccaat
    26221 ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat cgggcgacat tgttgctact
    26281 aaaataaaat ccaaatcttt agggtttaga tttttctgtt ttatcaattt ttccgccact
    26341 aaaatgcata aatcagaagt ctcctgattt gttacacatc ttcgttcacg aatacccgtc
    26401 cttgacgaaa tccattcatc agaggtatcc atcatcttgg aaagatcatt attagtaata
    26461 atcttttcag gaaggacgct ggctgttgca gtaatcattc caaattgttc cattaaatat
    26521 cctcacttat agtcttgcaa gaagtcgtgc aagtttttca gtgcatccag caaggcatgc
    26581 tgttcctccg tagccattcc gtctaagatg ttcttgacca tttcacgatg aaaatgctga
    26641 tggacccgaa atagtaactt tccttttttc gttagtccca actttacaac gcggcggtca
    26701 tcttcactgc ggatacgttc gacatagcct ttttttacta acttgttaat agcagtggtc
    26761 aaggtcccaa cagtaattga tagttctttg gctacttctg aagttgtttt tttcttgtac
    26821 atcccgattg cctcgatcgt gtgcatctct gtgattgaca gatctttgaa ttgagatttt
    26881 ttcagctcag actcttcgat tgtcaaaata tcattgaaga cactaacaag ataatcattt
    26941 actgttttta aatctggttc catgttccac cgtccactaa ttagtttgat tatcaaatct
    27001 tttgatgttc aaactatatc ttaacttttt tcaaaatgca accttaaaag atcatttttt
    27061 tactatatga gcttttttta agattttact catttaaaac aactgataaa tcagcattta
    27121 taatttcttt tattagaaaa atcatctatc tcacatattt tgattatcaa agtattttct
    27181 gtgaacagaa ctatagaatt ccctaaaact tgtccattat ttcaaaattt gttatagtct
    27241 ttttattcaa agcgtaaaag gagatcctat gttaaaagaa aaaatgaaaa ctctccccat
    27301 gctgccgggt gtctatctta tgaaagaccg agatggtaag gtcatctatg ttggtaaagc
    27361 aaaacggctc aaaaatagag taagcagcta ttttcatcag aataaacagc attcaaaaaa
    27421 agtattacgt atgattcatc atataacaga ttttgatttc gtcgtagtag acacggaatt
    27481 agatgcttta ttgctcgagt gtcaattgat ccaacattac cgtccttttt ataaccgaca
    27541 aatgaattat ttcagtaatt ataattatgt acatattacc aataagggat ttgtattaac
    27601 agatactcct acggctcgaa catacgggcc ttttcgtctc tataaaaaaa tgccgtctat
    27661 tttgcgtata atggaagaaa cttatcaaat gccttggttg tctgaaatta gtttgttagc
    27721 tttgcgagtc cagcttcctg acctacagga aatgactttt gaacagaaaa aaaaggaact
    27781 ccaagggctg tttcaaggcc gaaataaaaa gctactgact tacttgaaaa aaagacaaca
    27841 acatttcatc tatcagttga atttcgaaaa agccggtatg cttcaaaaag atatcgaatt
    27901 agttacttat tttattagac ggatccagga acaaaagcag tttttaagaa caccatcgct
    27961 caccttcagc atgccattag cagcagatga gagtcagaaa aagcactacc tcgtttgtta
    28021 tggtcaactt gcagaaacaa tgattacctc tggtgacgtt tctcctgact tctattatga
    28081 aaagaaagaa gctcatttat ctttgaaacg tcaattgtca aaagaagaaa tcgatcctgt
    28141 acaaatcttg atcagttacc gcaaaaaatt agaaaaagaa cagtttgaaa ttgaactgtt
    28201 aaacaaaaaa gaagctgaaa agcaactaaa ttagttgctt ctcggcttct tttaggcatc
    28261 ttcaacttca aatgtcaatt atcctaaaat ttattggatt aagtcataga tttccatcgc
    28321 tacgatatcg atattatcaa attcgtattg ctgatgagta tctgtttcag ttaattcaaa
    28381 tgtatcggtc gctttatcat aagttactgt acacttctct acgccttctt tttcaaaacg
    28441 gcgaacttgc agttcattat ctgctgactc gaccatagcc tccagtcgtt ttatgattgc
    28501 gactaattgt gaatgtgtca tcggaggtcc tccttttctt atttctgtta tattgtatca
    28561 aaagcatagc ctacttgcat tgattttctc taaaaaaact cgttttttat gatttttttt
    28621 ctggtatttt tccttcaggc caccaaagtt tgatcaaagc ttgggttccg tcattctcaa
    28681 aacctttatc tgccaactct tcatatagtt tcaatgcttg ggtagttgct ggcaatacaa
    28741 gatccatttt ttctgcttct tctaatgcaa tctttaaatc tttgataaaa tgtttgacaa
    28801 aaaaaccagg tgtgtaatct tcttttaaaa tacgaggaga ataattgctt aaagaccagt
    28861 tagctgcact tccacctgaa agcgtctcaa tcactttctt taaatcaagt ccgttttttt
    28921 gtgcatagac aagcatttcc gtcattcctg tcatcgtacc tgcaatcatg atctgattag
    28981 ccatttttgt atgctgacct ttcccagcag aaccatgcaa tgtaaatgtc gtaccaaatt
    29041 ctttgaataa aggcagtact tgatcataaa cggcttcttc tccaccgacc atgatcgtca
    29101 gtgtaccatt ctttgctcct aagtcaccac cggataccgg tgcatctaac gcatctgcgc
    29161 ctttttcttt tgctgttttt gcaatttttt ctgccaggct tggtgtactt gtagtcaaat
    29221 caactaaaat atgtcctgag atatctgcag aaaaaatccc cgactgtcca tagtagattt
    29281 cttctacatc tttggggtaa cctaccattg taaagagaat attactttgt tctgctaccg
    29341 cttttggtgt atctgcccag atagctcctt ctgttactaa aggatcagct tttgcttttg
    29401 tccgattata gacagctact tcgtgtcctg cttctagtag atggcgtgcg acagcacttc
    29461 ccatgacgcc tgttccgata aaaccaagtt tcattttttc gacctcctaa aaatcaaaaa
    29521 tccttgcttc tatgctagta tacgctagaa acaaggattt tttcataaaa aatgtttgaa
    29581 ttttatcttt taaatgaatt taattttatt ttgattcgtt caatcgagtc ttcttcttct
    29641 tttcttcgca tctcttcttc atgataaaca accatgtctt gataaaattc gatcatagct
    29701 tcgccgaaat gtgtagatga aatatcataa agtttagctt ctaaaacttt tggatcttta
    29761 ggaacttcac tggaaatata atcaataagt gctggtgcaa agtcttcgtc ggtttcaaaa
    29821 gttttcccta atgaaggatg atcgaacaat cggttcaaat aagcatttcc ttccacgaca
    29881 caaggtgttc cggctgccat cgcttctgta taagtaagcc cttgtgtttc tgaagtagaa
    29941 gcactgacaa aataatctgc cgctttataa taaagtgcta catcattgtt gtccacttct
    30001 cctgtgaact gcacaaattc ttctaactgc agttctgaga tcaattcctc taactcttct
    30061 acataaggtc cttttcctac gataactaat ctggcgttag gataagcgga gatgacctga
    30121 ggaaacccat taatcaaggc ttggatattt ttctcataag aaatcctgct taaagaaaga
    30181 atcatcaaat ggttttcctg aagacctagt gactccctta atttgctgat atcctgctga
    30241 gtgatatcag aacgtttgaa tttttcaatg tcgataccag taggaatcac gcgaagcggt
    30301 gctattacac catagctgcg taatgtatca ataacacgtt cacttggaca aactacacct
    30361 gtcgtatgat ttgcaaataa tctagacaag tattttacat gggattgcct gatcactttt
    30421 cctttaccga tataatgaag gtagtcttca tacatcgtat ggtaagtatg gatcagaggt
    30481 attttcaact tcttagctac catcttccca agtatccctg ctccgaattc tgtatgggta
    30541 tgaataagat ctagttctag ttctttggca accaaataag cataccacat gcctctaaca
    30601 acaactcttc gatccttgaa agaaataaat ggtacacttg gcattcgaat cacatctttt
    30661 tccaacttat ccgcattagg atcggtcgtt gtaaaaatat agacttcatg cccatgtctt
    30721 tctaattctt gttttaatgt tttgatcgat gtagcaactc cgctgacttg cggaaagtag
    30781 gtatcggtaa aaaagccgat tttcatgttt ttcgcctcca aactagtcct tgacctgatt
    30841 tacttttttt aatttgctag agactttttc attcagtaca ctttcgtata catttttaag
    30901 ttcttcaccg attttattga tacttcgctc ttttgctgtt tgataaccgg cttgtgtcgt
    30961 actcggtagt tcttgattgg taatctgttg gatcagtcgg ataaattctt cgtttgtctt
    31021 ccccatatag cagttcttgc tatcttctaa ccagccatta tacactggaa tatctcgaac
    31081 aagtacgttt tgtttgcttg ctaaagcttc taaaacaacg atcccttctg tctcttcata
    31141 agaagggaaa aagaatagat ctgcacctga atacgcacct tctatgatct ctccacgaat
    31201 gtaaccagga aacaggacat tttcaggatg atcttctttt acaattttcc ggatgttccg
    31261 aggaatcgag tacatgggaa catgaccaaa ccaaataaat gtgtattcgg gcatcttctt
    31321 agcaatttct acgaaatcaa tgattccttt acgttcgaaa aacaaaccga cacaaatgat
    31381 tactttttga tccggagaca gtttgaaata ttctctgaac ttttgttctt tcattggatc
    31441 agggtgatat ttctctaagt cgatcccatt ggaaatagct tgaatcggca cacggatacc
    31501 atagctttcc aacaactgtt ttgaataagg tgttggcgtg atcagatagt ccgctttttg
    31561 gtacaatcct accagatatt tttttacgat aggagaaagc tggttcgatc cgataaagga
    31621 atttcggaaa tcttcctctg tagaatgcgc atgatagaca actttttttc cttctcttct
    31681 ggctttattg accatattat gactattgat tccgtatgta ttgatgtgca ggatgtcata
    31741 atcttctttt tcatctagtg tataagagat accgacttcc gtcagagcac gtttttgatg
    31801 atcaagcgca cgcccgattc cagattttgc aagcatcttt tcactttcaa aatataataa
    31861 taccttcacc ttacgtcccc cttcacttat atagtataac atagcctaca aactgtcact
    31921 caaacttaag ccctattttt gtctattctt actgacaaaa acgttttctt aaaaaagaac
    31981 aaaaaaactg gcgtagctct tttacaagcc acaccagctt ttgatacgtt gtttatttta
    32041 catattcatg aacaagttca acgacttctt ccatcatatc gcattctttc aatgcacgat
    32101 cagcaagttc agccattttc gctgtgtcca agcgtttcat taaactacgc gttttaagaa
    32161 tagatgtcgc actcattgag aactcatcta gacccatacc tactagcaat ggaacagctg
    32221 tttgatctcc agccatttct ccacacatac ctgcccattt tccttcagcg tgtgctgcat
    32281 caattacatt tttgatcaaa cgtaaaattg atgggttata tggttggtac aagtaagaaa
    32341 cgcgttcgtt catacggtct gctgccattg tgtattgaat caaatcgttc gtacctacag
    32401 agaagaaatc tacttctttt gcaaacttgt ctgctaagac tgctgctgct ggaatctcga
    32461 tcatgatgcc gacttggatc gtatcagata cttccttgcc ttcacttatc aatttttgtt
    32521 tttcttcttc aaagatcgct tttgctgcgc ggaattcttt gagtgtagct accataggga
    32581 acatgatacg taaattacca tgaacagatg cacgaagcaa tgcacgcatt tgtgtacgga
    32641 acatttcgtc gccttgttca gataaactga tacgcaatgc acgatatcct aagaatggat
    32701 tcatttcatg aggtaattgc agatatggta gttctttatc tccaccgatg tccattgtac
    32761 gaacaactac aggttttcct tccatacctt ctaatacagc tttgtaagct tcatattggt
    32821 catcttctgt tgggaagtct ggagaatcca tatacaggaa ttcagtacgg tacagaccaa
    32881 ctgcttctcc accattatta tggacaccaa ctagatcttt tggtgttccg atgtttgcag
    32941 ctaattcaaa atgcttgccg tcagctgtga cagtttctgc attttttaat ttttcccatt
    33001 ctgctttttg tgctgcgtaa tctgcacctg ctttttcaaa ttcagctttt tgttcatctg
    33061 taggatcgat gattacatcg ccttcgatcc cgttgacagc caaaatatcg ccttctttga
    33121 ctttagcagt aatttctttt gtcccaacga ttgctggaat ttctaaagaa cgtgccatga
    33181 tagcagaatg tgaagtacgt ccaccaatgt ctgttacaaa cgcttttaca aaattgcgat
    33241 ctaattgtgc tgtatcactt ggagtcaagt catgtgcaac aacgaccact tcttcgttga
    33301 tcattgatgg atttggtaaa gtaacgccta ataagtgcgc taaaatacgt ttagcaacgt
    33361 cacgaatatc tgcagcacgc tcttgcatat aagcattatc ttccattgct tcgaacatac
    33421 cgatatacat atccgttact tcttttagcg ctgattcagc atttacgctg ttgtctttaa
    33481 tattttgctt gatttgacct accatttctg gatctgaaag aaccatcaaa tgggcatcaa
    33541 atacttgcgc ttctgcttca cctaagcttt gcgctgcttt ttcacgaatt tgctgcaatt
    33601 cttcagtaga tttagctaat gcagcatcca aacgagcttc ttcagccgca gtatcttcta
    33661 ctgttgcctt actgaatgtt aaatccggtt gaactagcag gtaagctttt gcaacggcta
    33721 caccgtcact agcggcaatc ccttttagca tttcaaccat tattcagata agccttcttt
    33781 cttcattgtt tcaacgattg ctgccattgc atcagcttcg tcagcacctt ctgcagtaat
    33841 agtaacatca gaaccttgac ctacacctaa agacataacg cccatgattg atttcaagtt
    33901 tactgattta cctttatact ctaagtttac atcagagtta aatttgcttg ctgtttgtac
    33961 taatagtgta gctggacgag cgtggatccc agtttctgct actacgtgaa attctttctt
    34021 ttccatatta atcggtctcc tttaagtgag tgttgttttt atgtgagggt tttctttttt
    34081 agtaattcag agaatcctaa caaatgccac cctttcatct gtaaagatta acattttttt
    34141 tcgttagata caacagtttt cattcaattt cagataaaag
//
LOCUS       DACDIP010000023        32747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDIP010000023 DACDIP010000000
VERSION     DACDIP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.023"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..418
                     /locus_tag="IV257_001299"
     CDS             188..418
                     /locus_tag="IV257_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8814442.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            515..1816
                     /gene="murA"
                     /locus_tag="IV257_001300"
     CDS             515..1816
                     /gene="murA"
                     /locus_tag="IV257_001300"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8814443.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1829..2005
                     /locus_tag="IV257_001301"
     CDS             1829..2005
                     /locus_tag="IV257_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8814444.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2140..2436
                     /gene="yidD"
                     /locus_tag="IV257_001302"
     CDS             2140..2436
                     /gene="yidD"
                     /locus_tag="IV257_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8814445.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2621..3088
                     /locus_tag="IV257_001303"
     CDS             2621..3088
                     /locus_tag="IV257_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8814446.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3173..4057
                     /locus_tag="IV257_001304"
     CDS             3173..4057
                     /locus_tag="IV257_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8814447.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4407
                     /locus_tag="IV257_001305"
     CDS             4174..4407
                     /locus_tag="IV257_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8814448.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4495..4575
                     /locus_tag="IV257_001306"
     tRNA            4495..4575
                     /locus_tag="IV257_001306"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4529..4531,aa:Tyr,seq:gta)
     gene            4582..4653
                     /locus_tag="IV257_001307"
     tRNA            4582..4653
                     /locus_tag="IV257_001307"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4614..4616,aa:Gln,seq:ttg)
     gene            5168..5875
                     /locus_tag="IV257_001308"
     CDS             5168..5875
                     /locus_tag="IV257_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8814449.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5934..6578)
                     /locus_tag="IV257_001309"
     CDS             complement(5934..6578)
                     /locus_tag="IV257_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8814450.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6704..6991
                     /locus_tag="IV257_001310"
     CDS             6704..6991
                     /locus_tag="IV257_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814451.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7099..7419
                     /locus_tag="IV257_001311"
     CDS             7099..7419
                     /locus_tag="IV257_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8814452.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7500..8813
                     /locus_tag="IV257_001312"
     CDS             7500..8813
                     /locus_tag="IV257_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8814453.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8810..9529
                     /locus_tag="IV257_001313"
     CDS             8810..9529
                     /locus_tag="IV257_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8814454.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9657..10208
                     /gene="raiA"
                     /locus_tag="IV257_001314"
     CDS             9657..10208
                     /gene="raiA"
                     /locus_tag="IV257_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8814455.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10430..12964
                     /gene="secA"
                     /locus_tag="IV257_001315"
     CDS             10430..12964
                     /gene="secA"
                     /locus_tag="IV257_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8814456.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13090..14191
                     /gene="prfB"
                     /locus_tag="IV257_001316"
     CDS             join(13090..13152,13154..14191)
                     /gene="prfB"
                     /locus_tag="IV257_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8814457.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14296..14982
                     /gene="ftsE"
                     /locus_tag="IV257_001317"
     CDS             14296..14982
                     /gene="ftsE"
                     /locus_tag="IV257_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8814458.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14966..15859
                     /locus_tag="IV257_001318"
     CDS             14966..15859
                     /locus_tag="IV257_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814459.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16011..17459
                     /gene="cls"
                     /locus_tag="IV257_001319"
     CDS             16011..17459
                     /gene="cls"
                     /locus_tag="IV257_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8814460.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17796..18653
                     /locus_tag="IV257_001320"
     CDS             17796..18653
                     /locus_tag="IV257_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8814461.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18768..19688
                     /gene="pstC"
                     /locus_tag="IV257_001321"
     CDS             18768..19688
                     /gene="pstC"
                     /locus_tag="IV257_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8814462.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19688..20572
                     /gene="pstA"
                     /locus_tag="IV257_001322"
     CDS             19688..20572
                     /gene="pstA"
                     /locus_tag="IV257_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8814463.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20587..21393
                     /gene="pstB"
                     /locus_tag="IV257_001323"
     CDS             20587..21393
                     /gene="pstB"
                     /locus_tag="IV257_001323"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8814464.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21409..22167
                     /locus_tag="IV257_001324"
     CDS             21409..22167
                     /locus_tag="IV257_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8814465.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22180..22857
                     /gene="phoU"
                     /locus_tag="IV257_001325"
     CDS             22180..22857
                     /gene="phoU"
                     /locus_tag="IV257_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8814466.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23094..24125
                     /locus_tag="IV257_001326"
     CDS             23094..24125
                     /locus_tag="IV257_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8814467.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24122..24889
                     /locus_tag="IV257_001327"
     CDS             24122..24889
                     /locus_tag="IV257_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8814468.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24989..26557
                     /gene="liaX"
                     /locus_tag="IV257_001328"
     CDS             24989..26557
                     /gene="liaX"
                     /locus_tag="IV257_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8814469.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26857..27174
                     /locus_tag="IV257_001329"
     CDS             26857..27174
                     /locus_tag="IV257_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8814470.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27174..27524
                     /locus_tag="IV257_001330"
     CDS             27174..27524
                     /locus_tag="IV257_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8814471.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27691..28629
                     /locus_tag="IV257_001331"
     CDS             27691..28629
                     /locus_tag="IV257_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8814472.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28644..29474
                     /locus_tag="IV257_001332"
     CDS             28644..29474
                     /locus_tag="IV257_001332"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8814473.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29518..30543
                     /locus_tag="IV257_001333"
     CDS             29518..30543
                     /locus_tag="IV257_001333"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8814474.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30558..31496
                     /gene="galU"
                     /locus_tag="IV257_001334"
     CDS             30558..31496
                     /gene="galU"
                     /locus_tag="IV257_001334"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8814475.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31621..32061
                     /locus_tag="IV257_001335"
     CDS             31621..32061
                     /locus_tag="IV257_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8814476.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32064..32666
                     /locus_tag="IV257_001336"
     CDS             32064..32666
                     /locus_tag="IV257_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8814477.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT        10867 a         5552 c         7097 g         9231 t
ORIGIN      
        1 tttggaataa gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag
       61 catttatttc ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct
      121 tttatacatt ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga
      181 cgtcattatg caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt
      241 tatctactta gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat
      301 gcataatacg caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc
      361 aagtaatttt ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat
      421 tttaaaaaaa ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag
      481 tgtatgctca ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg
      541 caatcaatta aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt
      601 agctgctagt ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc
      661 tgatgtcttc actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga
      721 acaaaagaat caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga
      781 gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg
      841 acatgcaaaa gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca
      901 tttaaaagga tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc
      961 aattgcggat gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac
     1021 tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc
     1081 tcgcgaacca gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta
     1141 tggagctggt actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca
     1201 ctcaatcgta caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca
     1261 aggcaatgtg ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt
     1321 gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa
     1381 acatatctta cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca
     1441 agctcaaatg acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt
     1501 gtttgaaaat cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat
     1561 cgacggaaat gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc
     1621 aacagacttg cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac
     1681 acgtgtgcgt aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca
     1741 gcaattaggt gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc
     1801 acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt
     1861 gataaagatt ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat
     1921 cggatatggt gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat
     1981 tcatattcga gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa
     2041 gtgagccatg aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg
     2101 atcccttttt ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat
     2161 ttagttagag gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac
     2221 ccaacctgtt ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg
     2281 gtaatgggga ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat
     2341 gtaccattga cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa
     2401 aaggttgtga aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc
     2461 ttatattttg caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa
     2521 aaaggttgtg aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc
     2581 aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa
     2641 gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt
     2701 atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact
     2761 gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta
     2821 tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact
     2881 cgcttccagt caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca
     2941 tctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca
     3001 ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat
     3061 tcacttacct aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat
     3121 gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat
     3181 attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat
     3241 taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt
     3301 tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc
     3361 tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca
     3421 tggatttaat attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa
     3481 tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga
     3541 tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat
     3601 caataaaggg gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa
     3661 gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg
     3721 tctcgataaa aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc
     3781 aatagcaaaa gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat
     3841 tcaattttcg tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg
     3901 gctcattgat gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga
     3961 tttcgaaccg cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc
     4021 agcaaactat ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt
     4081 gcataaaaga agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct
     4141 gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa
     4201 gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta
     4261 tttataggga aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt
     4321 ttctttggtg aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg
     4381 attcgacact ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg
     4441 caaaaatctg gaaaattagg tattataact aagtcaggtt gatacaaacc tttcggagga
     4501 gtagcgaagt ggctaaacgc gacggactgt aaatccgttc cttagggttc agtggttcga
     4561 atccactctc ctccatttca ttggggtata gccaagcggt aaggcaaggg actttgactc
     4621 cctcatgcgt tggttcgaat ccagctaccc cagtatacca caatcgtaag ctctcgttag
     4681 ttcgagagct cttttttttg ctttgctacc ttgttccttg aagattctgc tgatttaaga
     4741 tagagttaag ttaactacaa gaattggagg gatccacatg aaatggaaag aaaaagtaga
     4801 cgaagcagct gaaaagttat atgatttggt caaatcagaa aaatacaata ttgaagtgaa
     4861 tattccgaaa aaaggcggaa aagcggtccg ggtaaaatca aaacgcccaa cgaatcatac
     4921 aaaaaaatgg atggctaaaa accgctaata ataacaaaag atccataagt cggcattttc
     4981 atcgatttta gggtcttttg ttattttcta ggttggacat atctaaaaaa ttgtttctga
     5041 caataggagt aggaagaacg atgtgtgtct aaatcctctg ttgatggata gaaaccttta
     5101 tctttttcag ttgtgaatca ggtatatacc agttataata gaagaaacga aaggacgggt
     5161 gtacgttatg tcgaatcaat tacctgtata tatacagatt catgaccaat taaaaaatga
     5221 aatcgaacaa ggtatatgga aagtcggaga ccgtcttcct tcagaaagag aattagcagt
     5281 gaaattcaat gtcagccgta tgacgttgag acaagcaatt caaaacttgg cagatgaagg
     5341 aatcttagaa agaaagatcg gttcgggtac atatgttgca cgagaaaaag tacaagaaaa
     5401 aatgtctggt gcaacaagtt ttacagaaat tatggaatca caaaatcgag taccgtctag
     5461 tcggacaatc tcttatttct tgacttcgcc aagctcaagt gaaatggaaa aactaaactt
     5521 gagtaaagat gacacgatct tgcgaatgga acgtattcgt ttcgcagatg atgtccctat
     5581 ttgttttgaa gttgccagtg tgccacaaaa gctgatcgat ggctacagca aagcagagat
     5641 tactgaatcg ttgtatcgca cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa
     5701 tcaaacgatt actgctgtgt tggcttctga gaaaattgct gactatttag aggtaaaacg
     5761 aggagatgca gttcttcgtt tgcgccagat ttcttatttt gaagacggta cgccgtttga
     5821 gtatgtgcgt acgcaatacg taggaaatcg ttttgagttt tatttggaaa aataaaaaac
     5881 aggtgatccc tgattccgta ttagaaataa aagagaatca gaatcacctg tttttatata
     5941 tgatcagtta ccagttgctc gtgataagaa acaggaccct cttcaaattg gacttgtccg
     6001 ttcagcagct caatgatgga ttctttaaag tggtcagcac ttttttcatc tacagcacag
     6061 atgacgctaa cttgatcagt atattggatt tcttttataa aaaaatgttg gagttcaaga
     6121 taattctgta attttcctag ttgcggatac gtgatcatca cagagatctc ctgttgcaag
     6181 gtaccttcta cgattccaat gtcagacaat gcttgagaga ccgcatgagt gtatgctcgt
     6241 attaatccgc ctgtgccgag tttcgttcct ccaaaatatc gagtgactac tgcagctaca
     6301 ttgatcagtt gatttttttt gaggacttcc aacatcggta tgcccgccgt tccacttggt
     6361 tctccatcat cgctgcttcg ctggatttca tttttttctc caatgatgaa ggcgctgcag
     6421 ttatggttcg ctttccaatg ttcttttttt atttcttgaa tgaaactttt tgcttcgtcc
     6481 tctgattcta ttcgtctaag agaacagata aagcgtgatt ttttgatctc gatctcactt
     6541 tgtccatttt cttttattgt acgatagcgc tcgatcatct tgtcacctct tttttcattt
     6601 tctttattca gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaattg
     6661 ctgtagtatg cgataataaa gacaaaggca ggtggatctg gggatgcagg aacgaatgaa
     6721 aaagtacgat gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac
     6781 ctttacccaa tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga
     6841 aagtacagat tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat
     6901 aaatgcaggc tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa
     6961 attagtgaaa tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa
     7021 tttctttctt tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt
     7081 taaaaaggag agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg
     7141 agcacaagtt tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactct
     7201 gatattttcg ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agacgtggat
     7261 gtgttgttat taggcccaca agttcgcttt atgaaagctg attttgagaa acgtctggaa
     7321 ccaaaaggca ttcctttaga tgtcatcaat atggctgatt atggtatgat gaatggcgaa
     7381 aaagtgttag atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga
     7441 acaagcgatc caaaaaatag atcgcttgtt ttttcgtata tttttttagg gcgaataata
     7501 tgaatcattt ttggggtaga aggcttctga cgagagatat taaagcaata gactgtgagg
     7561 aggtaaattt accaaattat gagaagatcg cagcagtgga acgtttgagc aactggattc
     7621 tttgtcatcg ctgtggaatg agaacgtccg tcattgatgg acagcttgca gattacggtt
     7681 atttttgtac acattgtcta tatttagggc gatgcgacac tcaacaagat ttatatttgt
     7741 ttgatcagcc tgaagcaaaa cctcgagaag tcgtcttttt ttggacagga cagcttactg
     7801 aaaaacaaac ggaaatctca aagagaatac tttgccattc agaaaaaaga catcatctta
     7861 tttgggctgt aacaggtgca gggaaaacag agatgcttta tcctatttta gtggaaactt
     7921 taaaagctgg aggaagagta gcgatttgta caccgcgagt cgatgtttgt aatgaattgt
     7981 ttttacgcta tcgttatgtt tttccagaag aaaaaatcac gttgctccat gggaatacag
     8041 caacggctta tacctttact tcttttgtta tctgtacgat tcatcagcta taccgttttt
     8101 atcggtcctt tgatttaata gtgatcgatg agatcgatgc gtttccttat aacggggatg
     8161 caggattagc tcatgctgtg caaacagcga ttaaaccaga cggacgattc atctacttaa
     8221 gtgccacacc tgataaaaag ttactaaagg agatcaagca gacgtttgac atccacaaac
     8281 ttgctttacg ctttcatcgg cgtttgcttc ctgaacccgt cttggttttc atgaataatt
     8341 ggcgacaaaa atgcaagaac aagaagaaga taaaatcttt ggtgcggatc attcatcaat
     8401 taattaaaga aaaccatatc cttttatttt gtccaagtat cacgatgatg catcgtttga
     8461 aagccacact tcaagaggct cttccgagat atgagatcac agatgtttct tcaagagaca
     8521 atcagcgggc ggaaaaagtc cagaaaatgc gagaagcaaa atatgacata ctgttaacca
     8581 cgatgatcct tgaacgtgga gtcacatttg aaagaatatc cgttatcgtt ttaggagcgg
     8641 atcatccggt ttttactaaa tcttctttgg tacaaatcgc aggaagagcc gatcgtaaag
     8701 ggccttatac gaacagtcaa gtatattttt tctatgaaga aaaaactgca gctattcgaa
     8761 aagcttgtca ggaaatcaaa gaaatgaatg aagaagggaa gaagatactg tgaggtgcag
     8821 ttgctgcagt aaagatatcg tccaaaattt aacattcagc gagattattg gtgtttcgaa
     8881 atacagatta cctcagactt gttcagtttg tcgcgcacaa tttcatttat tggaaacaag
     8941 aggctgttct ggctgtcaaa aaccaatcca tttcaatcta gagagtacga aaaagaaagt
     9001 aaattctatc cccgtttact gcagtgactg tcaagaatgg aagaagaaat atccgaccta
     9061 tgattttcag caccgtgcct tttttgtgta cgatgaaaat atgaaggcct ggctcaatca
     9121 atacaagttt atgggagata tccgactagc aggcaccttt gcatataaat ggacgcagat
     9181 aaaaaaagaa tacccgacat acatctattg tccaatacct ttatctgaaa aaaggcgaac
     9241 agaaagagga ttcaatcaag taagcgagat gttgaaagca gcaagaattc cttttcagat
     9301 gctactcaaa agagagaagc atctgctggc tcaagcaaaa aacacgcgaa aggaaagatt
     9361 ggagatgcct caaccttttg aacttgcagt caccttttct aagatacaaa ataaaaaaat
     9421 attgattatt gatgatgtct atacaacagg acagactctt ttccacgcag cagattgttt
     9481 gcagatcgca cagccgaaaa tcatccgaac tatttctttg gcgagatgaa ttagatgtgg
     9541 aaatttgata gcgatttaat ttgcaaataa cctagagttc gatataatag aattaagaag
     9601 agggaaagag tggtccttct ccttcttgat tgtggtagac gaaaggggta atttttatgt
     9661 ttagatataa tgtacgcggc gaaaatatcg aagtaactga agcgattcgc gactacgttg
     9721 agaaaaaagt aggcaaacta gaacgctatt tcagtgacgc acccgatgca acggcgcatg
     9781 tgaacttaaa agtttataca gaaaaaacag caaaagtgga agtaacgatc ccattacctt
     9841 atcttgtatt acgtgccgaa gaaacttctc cagatttata cgcaagtatc gatttggttg
     9901 ttgacaaatt ggaacgccaa atccgcaaat tcaaaacaaa aattaatcgt aaatcccgtg
     9961 aaacgggcat gaatactgca aatgctgctg ctttattcaa tgaagaggac gaaaacgaaa
    10021 atgattctga attagatatc gtacgtacga aacgcctttc attgaaacca atggatagtg
    10081 aagaagcagt cttgcaaatg aacatgctgg gacataactt ctttattttt gaagatgcag
    10141 aaacaaatgg tacaagtatc gtttaccgcc gtaaagatgg aaaatacgga ctgattgaaa
    10201 cagactgatc agccttactt aaataacaac gaaaacttgc agcagctact gacagctgcc
    10261 gcaagttttt ttgagtttcc tcctctttta gatgaaatat gaataaatta tgaagttctt
    10321 actttaatac gcttaagata tttcaattat ctataactaa tgataaaatg aaaagtaaag
    10381 tctacatagt ctggataata cacagaagga aaaggagatt acacaacgca tggccaattt
    10441 gattcgttca cttatcgaaa acgataagaa agaactaaaa cgtttagata gcattgcaaa
    10501 aaaagttgaa gcatacgctg accaaatggc agcattaact gatgaacaat tacaggcaaa
    10561 aactgctgaa ttcaagggca gataccaaaa aggagaaaca ttagatcaat tacttccgga
    10621 agcttttgca gtcgtccgag aagcagcaaa acgtgtatta ggtttatatc cataccacgt
    10681 acaattgatg ggtggaatcg tgcttcacga tggaaatatc cctgaaatgc gtactggtga
    10741 aggtaaaacc ttgaccgcaa cgatgccagt atatctaaat gctctgactg gcgaaggggt
    10801 acacgtagtt accgtcaatg aatatttagc aacccgtgac tcgacggaaa tgggcgaact
    10861 ttataatttc ttaggattaa cagtggggtt gaatatcaac tcaaaatctt cggatgaaaa
    10921 acgcgaggct tataattgtg atatcactta tagtacgaat aatgaattag gatttgacta
    10981 cttgagagac aatatggtcg tttaccgcca tcaaatggta caacgtccgc tgaattatgc
    11041 cattgtcgat gaagttgact caatcttgat tgatgaagcg agaacgccgc tgatcatttc
    11101 agggcaagca gaaaaatcca cagctttata tacaagagca gacaacttcg tgaaacgttt
    11161 gaaagaagaa gaagattata aaatcgatgt acaatcaaaa acaatcagtt taaccgaagc
    11221 agggatcgaa aaagcagaag aaacattcgg tttggataac ttatacgaca ttgaaaatac
    11281 tgcattgacc catcacttgg atcaagcatt gagagccaac tatatcatgc ttttagatat
    11341 cgattatgtt gtccaagacg gaaaagtact gattgttgac cagttcactg gtcggatcat
    11401 ggatggacgc cgttattcag atggacttca ccaagcaatc gaagctaaag aaggcgtaga
    11461 gatcgaagac gaaacgaaaa ccatggcgac gatcactttc cagaactatt tccgtatgta
    11521 caagaaactt gcgggaatga ctggtacagc taaaacagaa gaagaagaat tcagagaaat
    11581 ttataacatc caagtatacc agattccgac aaatcgtccg gttatccgtg atgatcgtcc
    11641 agatttgctt tacccaacat tacaaagcaa gttccacgct gtcgtgcaag atatcaaaga
    11701 tcgctaccgc aaaggtcaac ctgtgttagt cggaactgtt gcggttgaaa catctgagtt
    11761 attatcagat atgctgaatc aagaacgtat tcctcatgaa gtgctgaatg cgaaaaatca
    11821 ctttaaagaa gcagagatca tcatgaatgc tggtcaaaaa ggtgctgtga cgattgctac
    11881 caacatggct ggtcggggaa cagatatcaa gttaggactt ggggtaagag aacttggtgg
    11941 tctagcagtt atcgggacag aacgacatga atctcgtcgt atcgataacc agcttcgagg
    12001 acgtgcagga cgtcaaggag accctggtat gtcacagttc tacttgtcat tggaagatga
    12061 tttgatgaaa cgattcggtt cagaacgaat caaagctttc ttagatcgga tgaagatcgg
    12121 tgaggaagat gctgttatcc aaagcaagat gctgacgaaa caagtagaat ctgcacaaaa
    12181 acgtgtagaa ggaaataact atgacacacg taaaaacgtc ttacaatacg atgatgtgat
    12241 gcgtgaacaa cgtgaagtaa tctataaaca gcgacaagaa gtaatcatgg aagaaaaaag
    12301 cttatccaag ccactaatga acatggtcaa acggacaatc agtcgtgtag tagatgcgca
    12361 tacacaatta gagaaagaaa attggaattt ggaaagtatc atcgattttg ccggcagtac
    12421 tcttgtccat gaagatagta ttactcttgc agatatcgaa ggaaaaacag ctcaggagat
    12481 caaagagtat ctaaacgaac gagcacaaga agtatttaat accaaagcgg ctcaactaaa
    12541 tggtccagag caactgttgg aatttgaaaa agtcgtgatc ttgcgtgttg tggactctaa
    12601 atggacagac catattgacg caatggatca attacgtcag tcaatcggct tacgtgctta
    12661 tggacagaac aatccattag tcgaatatca aacagaaggc tataagatgt ttgaagatat
    12721 ggtaggcgcc atcgagtatg aagtcactcg aatctttatg aaagctgaga tccgtcaaaa
    12781 cgtacagcgt gaacaggttt ctcaaggaac agcttctcac tcagaagacg gagatgccaa
    12841 agaaaatagt aatactagcg cgaaaaaaca gccaatccat gtgaacaagg tcggaagaaa
    12901 tgatccttgt ccttgtggaa gcgggaaaaa atataagaac tgtcatggaa aaaatgcaaa
    12961 ctaatttctg acagcaggat tggtgagaca aattcttttt ttaagaattc gtctcacctt
    13021 ctttctgtta tttgctgatt ctgttagact agatataatg aaaaaagtat agtgaaagaa
    13081 ggaaaaaaca tggaaatatc cgaaattcga aatctcctga cagaaatgca ggaaaaggtc
    13141 acaagtttca ggaggtctct ttgacttaga tcagctggaa gaagacatcg cggaagcaga
    13201 agcaaaaatg gctgaacctg gtttttggga tgacagcgaa gccgcacaaa aagtgatcag
    13261 tgaaaataac gctaataaag aaacctatga ccaattccat tctcttgccg aagaactaga
    13321 agaaatcgaa ttgatggtgg agatgctgca agaagaacct gatgcagcaa tgcaagaaga
    13381 agcagaaaca cgtatcaatg cattagatga aaaaatgaag acgtatgaat tatctatgct
    13441 gttagacgga ccttatgatc gaaacaatgc gatcgtcgaa ttgcatccag gggctggcgg
    13501 aacggagtcc caagactggg gcagcatgct tttacgtatg tatacacgtt gggcagagca
    13561 gcatggctat caagtagaaa cactagatta tcaagcaggt gatgaagcag ggatcaagag
    13621 tgtgaccctg ctgattaaag gacataacgc ttttggttat ctaaaatcag aaaaaggtgt
    13681 tcatcgcctt gtacggattt cgccttttga ctcagcgaaa cgacgtcata cttctttttg
    13741 ttcggtagat gtgatgccag agcttgatga aacgatcgat atagaaatca atccagatga
    13801 tttgaaaatc gatacgtacc gagcaagtgg tgcaggtggt cagcacatca ataaaacaga
    13861 atctgctgta cggatcactc acttgcctac aggaacagta gtagctagtc aggcacaacg
    13921 ctcacaattg aaaaaccgtg agcaagcgat ggggatgtta aaagctaagc tttatcaatt
    13981 agaagtggaa aagaaagaac aagaagcagc tgcacttcga ggagaacaaa aagaaatcgg
    14041 ctggggttca cagatccgtt cttatgtttt ccatccctat tctatggtaa aagaccatcg
    14101 gacaaacgta gagacaggaa atgtccaagc agttatggac ggtgaccttg atttatttat
    14161 tgacggctat ttaaaaatgc atctacaata agaacccatt gttaaattga aataaaattg
    14221 taagattttg caagcatgtt gaaacattgc catgctataa tagctaaaga ctgaaaaaga
    14281 tatggagaga acgccatgat tgaaatgaag gatgtaatga agaagtactc aaatggtacg
    14341 accgctatcc gcaatctctc gattgtcatc gaacaaggag agtttgtcta tgtggttggt
    14401 ccgtctggag ccggtaaatc tacattcatc aaattgatgt atcgagaaga aaaagcttca
    14461 aaaggaactt tgaacgtcgc aggtcatgat ctaatagcaa tcaaaaaccg cgaagttcct
    14521 tatctacgca gagaaatcgg cgtggtgttc caagactata aattattacc aagaaaaaca
    14581 gtatatgaaa atgttgcgta cgctatgcaa gttatcggca gaaaacctcg agatatcaaa
    14641 aaacgagtaa tggaagtgct ggaccttgtt ggtttgaaac acaaagttcg agtctttcct
    14701 agcgaattat ccggtgggga acagcaacgt gtctctattg ctcgggcaat cgtgaatacg
    14761 cctaaagtac ttatcgcgga tgaaccgaca ggaaatctgg atccagaaaa ctcatgggaa
    14821 atcatgaaat tattagatcg gatcaatgcc caaggaacta ctgtcgtgat ggctacacat
    14881 aacagtacga tcgtaaatac gattcggcac cgagtaattg ctatcgaaaa cggccgcatt
    14941 attcgagatc aagcggaagg ggaatatgga tacgatgatt agaacctttt tccgccattt
    15001 attagaaagt atcaagagtt tgaaacgtaa cggctggatg acagttgctt cagtcagtgc
    15061 ggtaacgatc acgttgactt tagtcggtgt attcatggca gttatcatga atgcaactaa
    15121 attagctcag gatatagaag gaaacgttga tgtatctgta ttcgtagata tcggtacaaa
    15181 agacgaagag atgaaaaagc tagaaaaaga attgaatgac ttagatcatg ttaaaaaagt
    15241 tgaattttca agtaaagaac aggaattgaa aaaaatccaa aacgaaatgg gtgattcctg
    15301 gaaattgttt gaaggtgaca ataacccgtt atatgatgtg tatgtagtaa gtgctactga
    15361 tccttcttat acaaaacaag tagcagaaga agcagcagat ttgaagaatg tttctcgttc
    15421 tgattacggt ggagcttcct cagatcggat tttccaaatc gctggtgcag ttcggacatg
    15481 gggattagtt gcagcggcat tgctgctgtt cgtagcgatg tttctcattt cgaacacgat
    15541 tcggatcacg attttgtcta gacagcgtga aattcaaatc atgcgtttag tcggtgcaaa
    15601 aaatggatat attcgctggc cgtttttcct tgaaggtggc tggatcggtc tgatcggtgc
    15661 gattcttccg attttgatca tcacatttgg ttatgcatgg tttttccaat tgatcaatcc
    15721 atcattatta cgttcccatt attcattgat tcatccacaa aatatcgttt ggaaaatcaa
    15781 tcttttaatg gtcggcattg gtgtgatcat tggttcactt ggctcgatca tttctatgag
    15841 acgtttcttg aaattctaat accacaatat aaataaaatg agaaagttgg acagtttcgt
    15901 tccagctttc tttttttatt ggctaggaga atagaaggaa gcggcttttt tccctttact
    15961 ttaaaaaaca acttgggtat tcttaagttg gtagaggaag aggtgaagcc gtgaaaatta
    16021 tcgttgacaa tttttttacg atccttttga ttatgaatat tttgctttcc tttatcatcg
    16081 tttttcgaga aagaaaggaa actgctcaaa catgggcatg gctgctcgtt ttgatgttca
    16141 ttccagttgt aggatttatt ttatatattt ttttaggaag agggatttcc aaagagaaaa
    16201 tctttgattt gaagatccaa gggaaaatcg gtaagaacct ggaaattgaa gaagacaaac
    16261 aggcattgat gcgagggctt tatcctcatc cgcctactgg acaagtagat gtgaaacaat
    16321 tgatctatat gctcacggta tttgaaagta cgctctatac gactaaaaat gaaatcactt
    16381 tatttacaga tggtcgtgaa aaattcgatg cgctgattga agatatcaag caagcagaag
    16441 accatatcca cttccagtat tatatttatc gcagtgatgc attaggtgaa gaagttcgag
    16501 atgcattgac agaggcggca agacgagggg taaaagtcag agtccttctt gatgcatggg
    16561 gttccacaca agtttcttcc agcttttttc agaatttgaa aaaagcaggc ggagaaatcg
    16621 catttttctt tcctctattt gttccgtata ttaatcctcg gatcaattat cgcaatcatc
    16681 gtaaaatcgt agttattgat gggaaaatcg gatatacagg tgggttcaat gttggaaatg
    16741 aatatctagg attggtaaag aaatttggtt attggcgaga caatcatttg cgtatttacg
    16801 gagaagcggt gtatatcttg caaaaccgtt ttttgatgga ctggaattct cagcatacaa
    16861 aagaagtagg ctattctccc aaattttttc cgtccattca ctctactggt gatatagctg
    16921 ttcaaatcgt gacaagcgga ccagataccg aacatgaaca aatcaaaatg acttatttga
    16981 aaatgatttc gttagccaaa cgggagatat tgatccaaac gccttattac ataccggatg
    17041 gatcgattca tgaagcactg aagttggctc ttctttcagg cgtaaaagtt catatccaaa
    17101 tacctaacaa accagatcat cttctcgttt actgggcaac gtattctttc gctgcagagc
    17161 tgatcgagta tggagcaagg atcgaaacat atgagaatgg atttattcat gcaaagacga
    17221 tgattatcga tggtggcatt gtttcagttg gctcagcaaa tatcgatgtt cgttcatttc
    17281 gcttggactt tgaggtcaat acactgattt atgatgaacg aatggcttca cgtgtaagaa
    17341 aagcattttt tgaagactcg aaaatttcta cgcatctcac aaaagaaatg tatgaaaacc
    17401 gaggaataat catcaagatg aaagaaggat tagcaagatt gatttcgccg ctcctttaaa
    17461 acacaaaaag gacatgagaa ccatcaacag atggagatct cgtgtccttt tctaacgggg
    17521 ccatttctga gagtacgtca gaaatggaga aagcgtctcg catgagcgaa attaactttg
    17581 gggtatctct tgttttttgg ggaaagcaag aaataagcta atatatgcta ttaaaagaga
    17641 cacatttcaa agccagtata gcatagaaac ttttaatgag atagtttttt ttacaattat
    17701 gaggatttat tttgttattt aatgattttg attcgttttc atcaagtaat gaaaatgata
    17761 aaataaacat actttaggat taaaggagat ttctaatgaa aaaacttaaa ttattcttac
    17821 caataatagg attagctatt cttttatcag gctgttcaaa gtggattgat cgaggagaat
    17881 ccattacggc tgttggctct tctgctttac agcctttagt agaaacagtt gctgaaactt
    17941 accagacaga aaatcctgga aaattcatca atgtacaagg gggcggaagc ggaactggtt
    18001 tgtctcaagt acagtcagga gcagtcgaca tcggaaactc tgatttgttt gcggaagaaa
    18061 aatcggggat cgatgcctca gcattagtcg atcatcgagt agcagttgtt gggatcactc
    18121 ctattgtgaa caaggatatt ggcgtaaaag acatcagtat ggaaaacctg aaaaaactat
    18181 ttacaggcga gatcaaaaac tggagtgaag tcggtggaaa agatcagcca gtagttatat
    18241 tgaaccgtgc ttcaggaagc ggaacccgta gcacgtttga aaaatgggta ttggacggag
    18301 aaacagctgt tcaggcacaa gaacaagatt caagcggaat ggtgcgccag atcgttgccg
    18361 acacacccgg tgcaatcagc tatgttgctt tttcttatgt ttccgatgat gtagatacgt
    18421 tgaatgttaa cggtgtagaa ccgacagatg aaaatgtgac aacgaacaaa tggacgattt
    18481 gggcatatga gcatatgtac acaagagatc atccaaaaaa actgacaaaa gaatttttag
    18541 actatatctt atctgatgaa gtacaagata acatcgttgg agaactaggt tatatccctg
    18601 tttctcatat gaaagtcgaa cgtgattggc aaggaaatat cgttaaagag taatctttac
    18661 acaaaattta caaacttagc taaactttta catgttatta aaaccaaatt gatgttcaat
    18721 ttgtacactg agtgagtatg ttaaacctga ttaaggggga gtcatctgtg gaagatgtga
    18781 aaaaagtatt attaacaaaa tcaaaacgtt caaaaatgga acagcgtgga aaattcatca
    18841 gttttttatg tatcgcgctg atcgtgttgg ttgtattatc tattttttat tttgtagcta
    18901 gtaaaggatt ggccaccttt tttactgata agatcaatgt atttgatttt ctgtttggta
    18961 cagattggaa tccaagccaa atcggagcaa atgggaaacc aatggtaggg gcattgccta
    19021 tgatcacagg ttcgtttatt gtgacctttt tatctgctat tgttgccaca ccatttgcca
    19081 ttggtgcagc agtattcatg acggagatct ctcctaaaaa cggaacgaag atcttacagc
    19141 cggtcatcga gctgcttgta ggtattcctt ctgtcgttta tggttttatc ggtttatcag
    19201 ttatcgtgcc ttttgtcaga agtatttttg gcgggacagg gtttggtatc ttagctggta
    19261 catttgtgtt gttcgttatg atcttgccga ctgtcacaac gatgactgtt gatgcattaa
    19321 aggcagtacc acggcactat cgggaagctt cattagcgct tggtgccaca agatggcaaa
    19381 caatctacaa agtcgtgcta cgagcagccg tgcccgggat tttaacagca gtcgtttttg
    19441 gtatggctcg tgcgtttggt gaagccttag ctatccaaat ggtcatcggt aatgcagcat
    19501 tgatgccaac aggacttact actcctgctt ctacattgac gtccatctta acaatgggaa
    19561 tcggaaatac aatcatggga acagttgaaa ataatgtgtt atggtcatta gcgctgatct
    19621 tgctattgat gtcattattc tttaatatcg tgatccgtat gattgggaag aaaggagagc
    19681 tgaaataatg aacgcaaaac gctcagataa aattgctaca ggtgttttat atgtagtttc
    19741 ggggatcatc gtattgattt tagcagcttt actactttat atcttagttc ggggaatacc
    19801 gcacatttca tggtccttct tgaccgaacc ttccaaagcc taccaagaag gcggtggaat
    19861 cgggatccag ctatttaact cgatttattt gcttttgatc acgatgatca tcagttttcc
    19921 tatttcttta ggtgcgggta tctatctttc agaatatgct ggaaaaaact ggctgacaga
    19981 tgtgattcgc acgtcgattg aaatcttgag ctcattgcct tctgtcgttg tcggtttatt
    20041 cggtttcttg gttttcgtta ttcaattcca gtatggtttc tcgattattt ctggtgcatt
    20101 ggctttgact gtattcaact tgcctctttt gactagaaca gttgaggagt ctttacaagc
    20161 ggtccattac acacaacgag aagcaggatt ggctttaggt ttgtcacgct gggaaacagt
    20221 gataaaagtg gtggtttcag aagcgacacc agggattttg actggtgtga tcctaagttc
    20281 tggaagaatt tttggtgaag cagctgcatt gatctatacg gctggacaaa gtgcacctgc
    20341 gttagatttt acgaactgga atccattaag tgtttcaagc ccgatcagca ttttccgtca
    20401 agctgaaaca ttagctgtcc atatttggaa gatcaataca gaaggcacga tgcctgatgg
    20461 tgtttctgtt tcggctggcg cttctgccgt gttgatcatt gctgttttac tgttcaactt
    20521 cggcgctcgt gcgattggca aacgattata ccgaaaaatg acttcagcct aaggagaaga
    20581 agcaaaatga aaaaatataa ctggaatgaa acaaatgtca tcaccatgcc aaaagagaaa
    20641 gaagtagcac tatatacgga tgatctacat gtctggtatg gcgacaatga agcaatcaaa
    20701 ggagtggatc tggaatttga aaaaaataag atcactgcac tgattggtcc ttccggctgc
    20761 ggaaaatcta cgtatcttcg ttcgttgaat cgaatgaatg atgggatcgc taatacgaaa
    20821 atcactggga aaatcatgta tcaaggcgta gatattaatg cgcctcaagt ggatgtttat
    20881 gagatgcgca aacggattgg catggttttt caacgtccta atccgttcag caagtcgatt
    20941 tatgacaata ttacatttgc tttgaaacag cacggcgaaa aaaacaaaaa gaaattagat
    21001 gaaatcgttg aaacaagctt gaaacaagct gccttgtggg accaagtaaa agacagcttg
    21061 gacaaaagtg ccttggcttt atctggcggg caacagcaac gtttatgtat tgctcgtgcg
    21121 atcgcgatga aacctgatat cttgctgcta gacgagccgg caagtgcttt ggaccctatt
    21181 tcgacaggaa cggttgaaga aacacttgta aacttgaaag aagactatac gatcatcatc
    21241 gtgacgcata acatgcaaca agcggctcgt atcagcgact atactgcctt tttctatcta
    21301 ggaaaagtaa tggaatatga caaaactaac aagatattta ccagaccaaa aattcaggca
    21361 accgaagatt atgtgtccgg tcattttggt taagacggag gcctcaacat gacaaaagaa
    21421 attatttcat ctaaagatct tcatctgtat tatggaaaga aagaagcact aaaagggatc
    21481 gatctaagtt tcaatcaagg ggaaatcact gcgatgatcg gtccttctgg ttgcgggaaa
    21541 tccacttatc tgcgttcttt gaaccggatg aatgacctga ttccgaatgt aacgatcacc
    21601 gggagtgttt tatataaaga caaagacatc tatggtccaa aaacagatgt tgttgaacta
    21661 agaaaagaaa tcggcatggt attccagcag ccaaacccat ttcctttttc tatctatgaa
    21721 aatgtcatct atggattgaa attaaaaggg gaaaaaaata aaaaagtcct tgatcaagta
    21781 gttgaagaaa gcctgaaggc agcgtctgtt tgggatgatg tgaaagacaa attgcataaa
    21841 agtgctttat ctttatccgg tgggcagcag cagcgtgtat gtatcgcacg tgttttggca
    21901 gttaatccgg aaatcatctt attggatgaa ccaacaagtg cgcttgatcc tgtttctact
    21961 gggaaaatcg aaagtatgct gttagaatta agagagcggt atacgatgat catcgtgacc
    22021 cataatatgt ctcaagcttc acgtatttca gataagacgg cattcttttt agatggtcac
    22081 ttgatcgaat acaatgatac gaagaagata ttcatgaatc cagaaaaaca agagacagaa
    22141 gattatattt caggtcgatt tggataagga ggaaacggaa tgctacgtac acaatttgaa
    22201 gaagaattgc tgaatctcca taatcagttc tacgaaatgg ggatgatggt gagcagcgcc
    22261 gtgcacaagt cagttcgtgc atacatcaaa cacgataaaa tcttagccca agaagttatc
    22321 gaaaacgata taaatatcaa taacatggaa acacgcttgg agaagaaaag ttttgaaatg
    22381 atcgcattgc aacagccagt tacaactgat ttgcgtatga tcattacggt catgaaagct
    22441 agctcggatt tagaacgtat ggccgatcat gcggtgtctg ttgctaaatc cacgattcgc
    22501 gtaaaagggc agacgagaat accggaaatc gaaaaagaga tctccgatat gtccgattat
    22561 gtgaaaaaaa tggtggacaa cgtcctagtt gcttatgtca aaacagacga aaaagacgca
    22621 cgtacgatcg ccaatatgga tcaaagagtc aatgactatt tcaatcgaat ctataatgca
    22681 acaatcaaaa atatgcagga aaatcctgag acagtcatca gtggaacaga ctacttgaat
    22741 gttgccagtt atctagaacg gatcggagac tatgtgacaa acatctgtga atggatcgtc
    22801 tacttggcta cagggaaaat cactgagtta aatacgaacc acaatgagat tttctaaact
    22861 aaagcggtga ttctgccgtt tgtttaggaa aagaggttgt gaaatcagcg gttgtgaaaa
    22921 agctgttctg catcaagtaa taagaacagt caaacaaaaa tagctcctca tatttttcat
    22981 ttggctggac ttattaccgc agatgcaagc ttttgaacac tgtttattcg ggaaagggtt
    23041 tgtgacaaaa tatgtcacaa acccttttca tcatttcata gaaaggataa agaatgcaaa
    23101 aaaaaattag catcatcact atatttttta tttgtgtgct tacttcatgg gttattcagc
    23161 atcaagctgc tttatatgaa gtacctgtca tcgagatcga aaaagtacga ataaaagagg
    23221 atcatcaatt gattacagga agactgatga acacgaatca aaaaggagaa tcgatttatt
    23281 tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa tataaaagcg agacaaatgt
    23341 atctagtcca aatgcatgga gatgaatggc agctagtagc aaaaaaaaat gatggctggc
    23401 ttttcttctt cacaagtatc ttcattggcg ccatgttgct tattggcgga aaacatggtc
    23461 tatttgcttt aggaagtctc atcatcaata tcgttttact tttacttttg ttgctcatct
    23521 ttactaaaat agaagggatt tctctattaa atatatctat cctgttcgtc gttataggga
    23581 tgattattag catattggct ttagatggat ggaatagaag cagtataatc aaaatttcag
    23641 ctgcagttac gagtgttttt ttagcatttt ttatttgtct tttgacaatg aatctttgga
    23701 aagaccaagg actgcgctat gaggaactga attatctgac acgaccgtat cgttctgttt
    23761 ttctttctag cgtactgatt ggaacagctg gaggaacact cgataccgtt atcacagtca
    23821 ttgcgacatt agaagaattg actaagcgac aaccagaaat aacagcgaaa cagttatggc
    23881 tttcgggaag gcaagtgggt cgagatgtga ttggctcgat gaccaatatc cttttgttcg
    23941 tctatatcag tgggtcagtt ccatcgcttc ttttatattt aggaaatcaa tggtcgttca
    24001 aagaaacgat agaacagcat ttatcattgg aatttttacg cgtcattgct ggcagcttag
    24061 gaatagtatt atcgatccct atctcggtcc tcttttttct gactgtaagg aggctgaaac
    24121 gatgaatgta cttattatac ttgttgggat tttagcaatc agcgtgctac ttattggcgg
    24181 tacgcaaggt atgtatattc ttttaggctt attcatcaat ttagggatct ttttcttgtt
    24241 gttattcggt tatcatcaga agtggccgat actggcattg tcggtcatag gatttttatt
    24301 gattgctgtg gttatcttat tttttatcaa cggttataat ctaaaaatgc gtgcagcttt
    24361 tgtgagtata ctgatctttt tattttgttt cctactattg cttcctatca ctgatttttt
    24421 agcaatccaa ggttttacaa cgatagaact agaagaatta tctggcttag ataagacttt
    24481 agcgattgat tttagattac ttacgagatc gttgcttctg attagtctta gtggtgctgt
    24541 tttagatgcg agtgtagcaa tttctagtgg gacattcgaa gtatatcagg caaatccgca
    24601 attatctttt taccaattgc gccattctag tttcgctatt gctaaaaaaa tattggcgtc
    24661 aacagtaatg acacttcttt ttgcattcat gggaagttct ttagcgttga tcctctggtt
    24721 tattgattta gacattcctt ttcagcaagt gatcaatgaa aaatcatttg ttctagaata
    24781 tacgatggct attcttacca cattgtccgc actacttgtt cttcctttaa cctgcgtgac
    24841 tacagcatat ctctttacaa aaaagaaaga acaactcaag atggagtaaa agttacaact
    24901 taagtcctat gacattttta ctgaatcagt gcataatatg gatgtaagga aaagcttgca
    24961 aacaattttg cttaaggagg caatttccat gaaagaaaga gaacgtattt tagatttagt
    25021 gaaaaaagga atcctttcga ctgaagaagg cttagattta ttagaaagca tggcaactga
    25081 aaaagacgaa aagcaaatca aaaaagaagc agacaaggta actgctagtc ataaagaaaa
    25141 agatcaggca agtcaattga tcgataaact tgaaaacggc gaagaagaaa tttctgagcc
    25201 agttgatcca aaggaaagag aaagacaaga tcaagaaaac ttagaaaaga tcttagatga
    25261 actagcaact gaagcgaata aaacttcggc gcgtctagat gaagtaaatg ctcaattcgc
    25321 tgacaataag gcagcacgca atgagaaaca ggaagcattg atgcagctga atacaaaaga
    25381 agaattaggc gagttgacag aagaagaatt agcacagcgt cagacgttag aagcagaaat
    25441 caaagagtta gaagctagtg gcgatacact gttagaagaa caattgaaat tagaagcaga
    25501 attaaaagat atccgtaaga atcaatggtc tgagaaaaaa gaaacgttta ctgataagtt
    25561 tgaattgcca gatgattgga aagatcaagc aacagataca ctgaatcaag taggcgaaaa
    25621 aatgtcagaa gctggatcac agttaggaaa attcttgaag aaaacattcc aaactgtttc
    25681 agaaacagtg aatgacaaca tggaatggaa agatgtcagc ttgcgtgttc ctgggattgc
    25741 gacaacgaaa tttgagcatg aattttacta tgaagctcct gcagcaagta tccttgatat
    25801 caaggcagca aacggaaacg tgacgttaaa aacgtgggac agcaacgatg tgaaagttga
    25861 agctaagatc aaactttacg gaaaaatggg tgctgaacca tttgaagcat tctctgaacg
    25921 gagccagatt gaggttaatg aagatcatat ctccttccaa attccaaata aacgtgtgcg
    25981 tgcagacctt gttttctact tacctaaacg agtatacgac cacgcagcta ttaaacttct
    26041 aaatggcaac atcatgattg aaacattgga agctaaagat atttatacaa aatctacgaa
    26101 tggtaacatc atcgtggacc aattaactgc aacgatgctg gaagtagaag gtgtaaacgg
    26161 caatatcgat attcgaaacg ggaacatctt agattctatt atcgaaacag taaatggtac
    26221 agtgacattt ggtgcgacag cagaaaacct ttctgtgtct ttggtaaatg gtgatgtacg
    26281 tcttacgatt aaagaagata atttgaaaaa agtagaagca agttcagtga acggaaatgt
    26341 caaagtcgct ttaccagatg gcatcggttt ggaaggacat gctaaaacaa gtcttggaag
    26401 tatcaacagc cgtctgtcca attacgaagt agttcgtgag aaaaaagaac ggacaaacca
    26461 aatgttgcaa ttccgtcgtg tttcagatgg tgaaatcgcg caagtgcaac tttcaacgac
    26521 aacaggaagt atctatttaa aagatacaga taaataaaga ggttgtgaaa gagatgttca
    26581 gccttgagca ataagaagcg attttgtaaa atagcttccc ctattttaca aaattgtgcc
    26641 ttattgccga taggctatct cttgaacacc gtttaccaag agattatgaa cttgccaatt
    26701 atcgccgagt cacaaggaat aatttcaaaa aaagctcgtc acgttttatg aatatggtaa
    26761 tttgtgaccg agaattttgc aaaaagacac cgattaagaa gaaggttgtc acaacgattt
    26821 tcttcattta aaatagtaga acagaggtga gacatgatga aaaaaaagtt gacaaaatcc
    26881 aataaaaatg ttgttttgac agggacttta gcaggaatag gagaatacat cgggattgat
    26941 ccgactgtta tccgtgtgct ttatgtattc ctgagcttag ttgcttttgg aagtccaatc
    27001 attttataca ttttattagc tttgattatc ccatctgcgc gttctgcacg aacaaactat
    27061 ggacataaca atgcatatta tgaaaaaaac aattatcgtg aacaaactcg tgaacaaaca
    27121 aaaaaacgca aagaagcgga aaaaatcgat gatgacgaat ggagtgactt ttaatgtcgt
    27181 actttcaacg tattttagtt aatacgttaa cattcatatc attggctgtt ctttttcctg
    27241 cgatggtcca tgtgagcagt atttggacgg ccgttcttgc cagcttcgta ttgtctgtgt
    27301 tgaatatgtt tgtcaaaccg attttgacga tactgtccct gccctttaca ttagtcacat
    27361 tgggattgtt tacgtttgtt atcaatgcag cgattcttaa aatgacctcc ttttttgtgg
    27421 gagcagaaca ttttagtttt tcttcattcg gtgcagcgtt gttagtagct atcttgatgt
    27481 cgcttgtcaa tgtaattgtc agcgaacata acagtagaaa ataaaagaaa gctgtgccga
    27541 actatcggcg cagttttttt atttagaatc aggaatgcgt tttctattac attggtaatt
    27601 aaataaaaac gtttttttta attggtagta cttttggcta gacaatgata aaatgataga
    27661 gaacactcta atagaaatga ggtagcatga atgtcagaag ttgtaaaagt tagccaatta
    27721 gtgaaaaaat tatctttgga aatcgtgaca ggcgacgaga aaagcttaga ccgtgtcatc
    27781 gtaactgggg acatctcacg cccaggacta gagttgactg gttattttaa ttattattcc
    27841 cataatcgtt tacagctatt tggcagtaaa gaaatcagtt ttgcggaacg gatgatccca
    27901 gaagagcgtt tgatcatcat gagaagatta tgtagtgaag atatgcccgc atttattatt
    27961 tcaagaggat taccagctcc agaagaaatg atccaggcag caaatgaaaa tgggatagct
    28021 gtactgcgtt ctcccatctc aacttctcgc ttattaggag aattatccag ttatttagat
    28081 ggtcgccttg cccctcgaac aagtgtccac ggtgttttag tggatgttta cggattaggt
    28141 gtactgattc aaggtgacag tgggatagga aaaagtgaaa cagcgttgga gttgatcaaa
    28201 cgcggtcatc gattgatcgc agacgatcga gtagatgttt atcagcaaga tgaattaacg
    28261 gtgatcggag aaccgcctaa aatcctggaa catctgattg aaatccgagg aatcgggatc
    28321 attgatgtaa tgaatctttt tggtgcgagt gcggtacgtg gctctatgca agtccagttg
    28381 gctgtttatc tggaagcatg ggcaaaagat aagaaatacg accgtctggg ctctgaagac
    28441 acgaccgttg aaatcgcgga agtaggcatt ccacaaataa aaatccctgt caaaacagga
    28501 cgaaacgttg caatcatcat tgaagtagcc gcgatgaatt tccgtgcacg tacaatgggc
    28561 tttgacgcaa cgaaaacatt tgaagaaaga ttaagtcgct tgattgaaga aaactcaggc
    28621 aacgactagc aaggagtgac aatatgttag cacaaatcaa tcgagttgct tttgagcttg
    28681 tagggattcc tatctactgg tatgcattga tcatcgtatc aggtatcatt atcgccatgt
    28741 ggcttagcag cagagaggct gttcgggtag gattaaaagc agatgatgtg acagatttta
    28801 tgttgtgggg attgcctatc tccattattg gcgctcgctt atattatgtt ttatttgatc
    28861 ttccccagta tatagctgat ccaatccaaa tattcaatat ccgttcagga ggcttagcta
    28921 tttatggcgg actgattgca ggcgcgatca cgctgtattt ttttactaaa tatcatttta
    28981 tttctttgtg gactttttta gatatcgctg caccaagtgt attattagca caagcaatcg
    29041 gtcgttgggg aaatttcatg aaccacgaag catatggacc agacacgacg cgaagcttct
    29101 tagaaagtct gcatttaccg caattcatca ttgataatat gtatatcgaa gggatctatc
    29161 gtcaaccaac atttctttat gaatcacttt ggagtttgat tggatttatt ttgcttttgt
    29221 ttttacgaaa gaaaccacac cttttgaaaa aaggtgaagt ccttttatgt tatctaatgt
    29281 ggtatagttt cgggcgtttc tttattgaag gattacgtac agacagtctt tactttttag
    29341 gtgtgatccg tatttctcaa ttattatctg ctgtgttatt tgtcggtaca ttgattctat
    29401 ttatttggcg cagaaaaaaa ggaaatttac cggattatga ccgctcatta ggaaaaaatc
    29461 aacaaattat ctgattttta gagcgttaaa aatgaaaaaa atttcaggag tggtaaaatg
    29521 aattatagag tagcggtttt aggaccaggc tcttggggta ctgctttggc acaagtcctc
    29581 gccgaaaacg gacatgacgt tcggatttgg ggacatcgtg cttcacaagt agaagaaatc
    29641 aacacccaac acacgaatca acgttattta ccagatcatt gtctacccga ttccatccaa
    29701 gcattcacca atatggaaga tgcagtaaaa gacgcagatg cggtgctatt cgtcattcca
    29761 acgaaagcaa tacgttctgt tgcaaaagaa ctagtgccta aattgaatac aaagccagtg
    29821 atcatccatg caagtaaagg attagaacag gggacacata aaaggatctc tgatatcttg
    29881 ttagaggaaa tcccagtaga aaaaagaaaa gctgttgttg ttctttctgg accaagccat
    29941 gcagaagaag tagcgaaaca tgacatcact acgatcacag ctgcaagtac agatgcaaaa
    30001 gcagcaaaat atgtacaaga gctgttcatg aataattatt ttcggatcta taccaatcct
    30061 gatgtgatcg gtgtagagat gggggcagct ttgaaaaata ttattgctat tggtgctggc
    30121 gctattcacg gcttagggtt tggcgataat gcaaaggcag caatcatgac gcgcggatta
    30181 gctgagatca gccgtttagg tgtagcaatg ggtgccaacc cgctgacctt tatcggatta
    30241 agcggagtag gcgatctagt cgttacttgc acaagtgttc attctaggaa ctggcgcgca
    30301 ggcaacttgt taggacaagg gcagccccta gaagaggtgc tagccaatat gggaatggtc
    30361 gttgaaggtg tgaatactac ccaagcagcg atggaactag cccaaataat ggaaatcgaa
    30421 atgcccatca ctcaaacaat ctatgaagtg ctgtatgaac aaaaagaaat caaaacagcc
    30481 gcaaaagaaa tcatgttacg tgatgggaaa aaagaaaatg aattctcatt aacaattttt
    30541 tgaaggagcg aatgtatatg aaagtcaaaa aagcagttat tccagcagca ggattaggaa
    30601 caagattttt accagcaaca aaagctatgg caaaggaaat gttgccaatt gttgataaac
    30661 caacaatcca gttcatcgtt gaagaagcgt tgaaatcagg tatcgaagac atcttgatcg
    30721 ttacaggaaa agctaaacga ccaatcgaag atcactttga ttcaaatatt gaattagaaa
    30781 caaacctaag tgaaaaagga aagaccgaat tattgaaatt agtagaagaa acgacagatg
    30841 tcaacttgca tttcatccgt caatctcatc caaagggttt aggacatgcc gttcttcaag
    30901 caagagcctt tattggaaat gaaccatttg tcgttatgct aggcgatgat ataatggaag
    30961 acgaagtacc attgacacag caattaatcg atgattacga acgtactcat gcttctacga
    31021 tcgccgttat gcaagttcct catgatgaaa cttcaaaata cggaattatc aatccaggag
    31081 aagtattgga agatgggtta tacaatgtca aaaactttgt tgaaaaacca gatccaagca
    31141 aagcaccaag tgatttagct attattggac gttacttatt gactccagaa atttttgatg
    31201 tattgtccga gcaacaacca ggagccggaa atgaaatcca actaactgac gcaatcgata
    31261 cattgaataa aacacaacgc gtatttgccc gtaaattcac aggtaaacgt tacgatgtcg
    31321 gtgacaaatt tggttttatg aaaacaagta ttgaatatgg tctaacacat ccagaagtag
    31381 gtgcaccttt acgtgagtac atcctgtctt taggtaaaac attagaagca gaaggaagca
    31441 agatcgatca taatccaaaa ggaataacag aaatcaaaga agaaaagaaa aaataaccct
    31501 tttttctaac gagcttcagc tgctaattga ataaaaagta gttgaagctc gtttttattt
    31561 gtttaaaaaa cacgaaaatg ctatccttat agaaaagaga gacaagaagg gagtttgatc
    31621 atgtcaggag gagaaattgc aggattgatc gcagcaatcg cctttgcgat acttgtcgtt
    31681 tttatcgttc gtgtgttgtt gcaagtttct aaaactttag aaaaagtaga tcagactgta
    31741 gcagaagcaa atacgacgat cgaaatcgtc acaaaagacg tagatctttt gtctagacaa
    31801 gtagaaggct tactggtcaa aagtaatgaa ctgctcttgg atatcaatgg aaaagtcgca
    31861 acgatcgatc cattgttcac agcagttgct gatttaagtg aaagtgtatc agaacttaat
    31921 cattctagta aaaacatcgc gacgaaagtc ggcggaatcg gtaaaacgac agctaaagcg
    31981 acggttgccg gaaaagtcgg tgggacagcg atgaaatttt ttaaaaacaa aaaacatact
    32041 gaaacgacag gaggaaaata aaaatggcta aaaaaggtgg atttttatta ggtgcagtag
    32101 tcggaggcac agccgcagca atcgccgcgt tattgttagc accaaaatct ggtaaagaac
    32161 ttcgggatga attagctgcc caagcggatg atttgatgga tatggcttca gattacacag
    32221 atatggcgaa agaaaaatcg actgagttaa caggtattgc aaaagaaaaa gcgcaagatc
    32281 tttctcaaca agcaggtgat ctagcaggta gtgtcaaaca aaaagtaagt gaatcagcag
    32341 atgaagcagg aagtgaatct gacgatatct tgttaaatct taaagaacaa tcagctgact
    32401 tatctaatcg tttcaaaaaa acggcagaag atatgaaaaa acctgcagat gaattaggaa
    32461 gtattgctaa agatacttca gaagatatca tgattgatgt aaaagacgct gctggtaaag
    32521 caggagatac tgtcaatgaa ggaatgtcag aagcaaaacc agttgcagat gactcaaaag
    32581 aaatgatgaa aatggcaaaa gaagatgcga aagaagctaa aaaagaaata aaagatacaa
    32641 tgacagattc atctacaaat cactaaaatc acaaatttat agaataaaaa ggtgggagcg
    32701 gactagctct cacctttttt actcttattt ttctaaaaca agtaatt
//
LOCUS       DACDIP010000024        32611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDIP010000024 DACDIP010000000
VERSION     DACDIP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.024"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..482)
                     /locus_tag="IV257_001337"
     CDS             complement(12..482)
                     /locus_tag="IV257_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8814478.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IV257_001338"
     CDS             complement(641..1099)
                     /locus_tag="IV257_001338"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8814479.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IV257_001339"
     CDS             complement(1270..2415)
                     /locus_tag="IV257_001339"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8814480.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKRDEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IV257_001340"
     CDS             complement(2428..3345)
                     /locus_tag="IV257_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8814481.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IV257_001341"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IV257_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8814482.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="IV257_001342"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="IV257_001342"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8814483.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="IV257_001343"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="IV257_001343"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8814484.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="IV257_001344"
     CDS             6871..7752
                     /locus_tag="IV257_001344"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8814485.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="IV257_001345"
     CDS             7749..8465
                     /locus_tag="IV257_001345"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8814486.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="IV257_001346"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="IV257_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8814487.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="IV257_001347"
     CDS             9182..10183
                     /locus_tag="IV257_001347"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8814488.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="IV257_001348"
     CDS             10180..11541
                     /locus_tag="IV257_001348"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8814489.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="IV257_001349"
     CDS             11501..12652
                     /locus_tag="IV257_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8814490.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="IV257_001350"
     CDS             12627..13796
                     /locus_tag="IV257_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8814491.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="IV257_001351"
     CDS             13971..15350
                     /locus_tag="IV257_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8814492.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..16428
                     /locus_tag="IV257_001352"
                     /pseudo
     CDS             15631..16428
                     /locus_tag="IV257_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            16617..17285
                     /locus_tag="IV257_001353"
     CDS             16617..17285
                     /locus_tag="IV257_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8814493.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(17485..17871)
                     /locus_tag="IV257_001354"
     CDS             complement(17485..17871)
                     /locus_tag="IV257_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814494.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(17843..19225)
                     /locus_tag="IV257_001355"
     CDS             complement(17843..19225)
                     /locus_tag="IV257_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8814495.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(19381..20568)
                     /locus_tag="IV257_001356"
     CDS             complement(19381..20568)
                     /locus_tag="IV257_001356"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8814496.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(20575..21606)
                     /locus_tag="IV257_001357"
     CDS             complement(20575..21606)
                     /locus_tag="IV257_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8814497.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(21622..23076)
                     /locus_tag="IV257_001358"
     CDS             complement(21622..23076)
                     /locus_tag="IV257_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8814498.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPTLSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVASASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            23251..24156
                     /locus_tag="IV257_001359"
     CDS             23251..24156
                     /locus_tag="IV257_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8814499.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTGESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24247..24924
                     /locus_tag="IV257_001360"
     CDS             24247..24924
                     /locus_tag="IV257_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8814500.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            25205..26101
                     /locus_tag="IV257_001361"
     CDS             25205..26101
                     /locus_tag="IV257_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8814501.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(26156..27526)
                     /locus_tag="IV257_001362"
     CDS             complement(26156..27526)
                     /locus_tag="IV257_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8814502.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            27764..28510
                     /locus_tag="IV257_001363"
     CDS             27764..28510
                     /locus_tag="IV257_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8814503.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            28507..29346
                     /locus_tag="IV257_001364"
     CDS             28507..29346
                     /locus_tag="IV257_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8814504.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            29347..30297
                     /locus_tag="IV257_001365"
     CDS             29347..30297
                     /locus_tag="IV257_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8814505.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            30438..31190
                     /locus_tag="IV257_001366"
     CDS             30438..31190
                     /locus_tag="IV257_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814506.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            31209..32360
                     /gene="queG"
                     /locus_tag="IV257_001367"
     CDS             31209..32360
                     /gene="queG"
                     /locus_tag="IV257_001367"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8814507.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT        10636 a         5909 c         6732 g         9334 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcatctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagatt atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc gaggaaaagt tggagcagta gccaaaaatc ggatccatcg tcgtttctac
    15901 accagtatct gccatcagaa aatcacgacg gatacaactg aatttaaata ccatgaagtg
    15961 gatgccaaag gcattatccg tcaaaagaaa ttgtatctag atccttttat ggatatgttc
    16021 aatagtgaga ttttatccta tcgtatctct gaaaagccca atgcattagc ggtaatggaa
    16081 ggtttggagg aagctatcca gaccactaac gattgtccct atcgtcgtac gtttcactca
    16141 gatcaaggtt gggcgtatca aatgaaagct tatagaaaca aattgaagga gtacaaaatc
    16201 tttcaaagca tgtctcggaa agggaactgc ttagataatt cacttatgga gaattttttt
    16261 ggcttattaa aacaagagat ttttcacggg aaagtttata actgtttcga ggaattaaaa
    16321 tcagccatcg atagctatat ctattactac aataatgaac ggataaaaca gaaattaaac
    16381 tggcaaagtc tcgtccaatt tcgtaaaaca accgtaacgg ttgtgtaaaa atatcatttt
    16441 agagcataaa aaaaaggagt ggatttctcc actccaaaaa agtctaactt ttgggggtca
    16501 ctacagtgtc acagtccttt ttttagtaaa aggatacatt tcttccttgt atattccatg
    16561 cgtgttcgtg agaaagcata tataatttaa ttaacagata gttaggggga gaaaaaatga
    16621 ttttttcaat tacaatgaaa ctggtgttcg ggttgatcgg tttattatta gtggtacgtt
    16681 tgcttgggaa gaaatccatg tcagaaatca caccgtttga tttagtctat acgcttgttt
    16741 taggtgggat cttagaagaa tcaacctatg atgacaatgt ccatgtaggg catgtcctat
    16801 ttgccattgc gctttgggca gtaatgattt atggtatcga acgtatcgtc caaaaaaatg
    16861 aaaaggtcaa tcgttgggtc aaaggcgagc catctgttct aatcaaagac ggcgtcatca
    16921 acatgacaga gttgacaaat aaccatatcg agatggagca gttgcgagca atcttaagac
    16981 agcaagaatg cttttttctt gaaaatgcca aacatgttat cctagagaat gccggtcaaa
    17041 tgagtgtatt gaaaaaatca gatgaagaca aagctttatc gatcctgctt gtagatgaag
    17101 ggcaaattca gcataaagtg cttcagtcca atcaattgac tgaagcatgg cttatggaga
    17161 acttgaagaa agaaggctat gcagatgtaa aacagctgat ttatgttgaa tggtcagaag
    17221 aaaaagggct ctacatacta aataaagaag acgtcatgca tgaatcttac cgaatagatg
    17281 gatagataga cagaggttgt gaaatcagcg ccttggcttg ttactcaaaa ctaagcgact
    17341 ttttcacaac ctctgatatc taatatacgt tgttccagaa gttgcttctt cgaaaaataa
    17401 gccaaaattc cacaaaaatt taagaagcaa ttttcgaaaa atcctttctt attttttgaa
    17461 gttgatcact tctgtcacaa cctctcatgt cacaaaccat ttttttgttt cctttaactc
    17521 aatataccgt acgtcttccc acaatatcgg ctgatggtca atcataaagt aatcttcata
    17581 ggcttcacca atgaagtgcc cttcgacctc atccaagagt cttccaaatt catcttttaa
    17641 attgagctga atgattactg ctttacgaga taaaaaggct tctgtcagta tactatcgat
    17701 ttcttgcaat gtcatttgtt gtttaggaag aggctgtttg cgtgcttctt cttcattttt
    17761 tttgataccg ttgactagtt cttccatcgc aaaagcagtc ccccatttta atccaaatgg
    17821 ccgatctcga tactcgttat agtcaatgaa tttttttgct gtccgtctca tcttttccac
    17881 cttctaatcc actcatacca cctgcatgcc cgccaacgag agaactacgt gcgatggcgc
    17941 gtcctccttg caagagacta tgcgcatgga tgattgattg aaagccgtat ttttttctaa
    18001 tcgtatcgac aacaaaatca actttcgctt catttatctg cttttcagga tcttcaaaaa
    18061 ggttaagctg taaacctgtc gcgtatacga ggtcggaaca gttcacgcct acacgccgaa
    18121 tatcctggta gtggtaatgc tggtggaaaa tggtcaataa agcatctgct agatctctgc
    18181 tagcatttgt cgcttcgatt ttcatttgct ggtgaaaccc ggtcttgcca tttggatcaa
    18241 gatagcctaa agagtatcct acccataagc tgactacttg tgtttttgct gcagatcgtc
    18301 tcaagcgagt gccgacttgg tcggccattt ctttgataac gatctcgatt tcttctcgtc
    18361 ttgtataatc tctatctagg atttgactat ttccgatact ttttgatttt ggtgtatatt
    18421 cttgtcccaa aaaacttcga tcgatgcccc aagcatgggc ataaagctga gtaccgatga
    18481 cccctagctt gcttttcaag tgataataat ccgcgtgagc taaatcataa acagaatgta
    18541 tacccatttg tttcagacgt tttgctgttc gtttgccgat tccccaaaag tcagtgatat
    18601 ctgagatttt ccataacttt tctggtacat cttcataacg ccattcagca aacatttgct
    18661 ttccgtcttt tgagccattg tctaaggcta gtttagcaag tagcgggtta tcgccaattc
    18721 ctatagtcgt gtagattccg gtctgctcaa acacatcttt atggatcagt ttagccatgt
    18781 cgtacgcatt tgttttacca aagagtgcca tgctatttgt tacatcaagg aagctttcat
    18841 cgacggaata gacatgatgg ttcgcttcgt ctgcatattt tttatagata gaattgattt
    18901 ctctgttctt ttccatataa taggccatcc ttggggcaac aatatataaa tcacttggat
    18961 aggggaaggg aagatctctg gcacggctga tattggtgat gccgtaagct ttttttgctg
    19021 caggactgga agccaagatt aatccgctac cccgtgtttc tgtcgaatcg gacggatagg
    19081 acatgactac taatttagtt gtaaggggat caagatcctt ttcaacgcac tctacggagg
    19141 cgtaaaaaga tttgcagtca atacataaaa tatctcttga aggttctttt tcataaacaa
    19201 ataccggatt ttgattcgcc cacataacaa tcacctcttc aacacaatta gaacacacgt
    19261 tctctttccg tgtcaataaa aaacagcaat tttcaaatac gagcaaaagg ctcacatttg
    19321 gaaattgctg agaggttatt tttatctatt tgtttttagc tgagatcgtt gattttttta
    19381 tcaaacatga acagcttctg gatcgatctt tgttgtccca tttccaatgt gtgcaacata
    19441 aaattccgga gcatagccga caactttcat gtattcatca tagacattgt ttttgaaggc
    19501 aggtatattt tcttctttga caagagcaat cgcacagcca ccaaatccag ctcctgtcat
    19561 tcgtgcacct aagacacctt cttgtttttg agcagcatct actagtgtat ctaattcgat
    19621 cccagttact tcataatcat agcgaagaga gtggtgagaa tcatttaaca gttgaccaaa
    19681 tgcttctaag ttcccagctt ctaattcagc ttttgccttc aaagtccgct gattttcaga
    19741 aacggcatgg cgagctctgc gtatcaatgt atcatcgccg atttggtcaa tagttgattc
    19801 aaattcttct tcatctaagt ctcctaaaga agagataggc aaagtttttt gcaaacgtgc
    19861 caaggcttct tcgcattcgc tacggcgttc attgtatttt gaatctgcca attcccgccg
    19921 cttattcgta ttcatgatga cgattgcata accatctagt ttcactggaa ccatttcgta
    19981 tttcagcgta tttgtatcta ataagattgc atgatctagc tcaccgaagc cgatggcaaa
    20041 ctgatccatg atacctgaat tgacaccgat gaattcattt tctacttttt tacctgtttg
    20101 gactagttcc aagcgatctg tttttaaatc aaacagatct tctaatacaa cacctactaa
    20161 taattctaat gaagcgctac tggaaagtcc tgcaccatta gggattgttc cttccactaa
    20221 taactcaaac ccatgatcga ttgagtgtcc agcttctttt aatttaagga ccattccttt
    20281 tacgtaattt gcccagtttg cttttttatc atattctagc tcatctaaag taaaactaat
    20341 gacaccgata tcgtcaaagt ttgtggaata aactaatatt ttattatcgt ctcgaggtgc
    20401 tgctacacct tgcgttccat aagtgatgga agcggggaag acatggcctc cattgtaatc
    20461 tgtgtgttca ccaatcaaat tgatgcgtcc tggtgagaag tattgtgtag tttttttagg
    20521 tccgaatgtt tcggcaaatt ttgtgtttaa gcttcttttg atttccatga tgagttattc
    20581 ctttcttgtt ttgattttga attctgtcac tgtttcaaat actgatccgg cttttaaatg
    20641 aatcgaacca aatgctgaga agcgctctgc acctggagct gtttgagtct caaatgtgat
    20701 gccaccgtga tgtgccagtt ttcggttgcg catttctggt gtttctgtgc cgaaatttgc
    20761 agtgaaaata accacactag aagcatctgt agcaatatct acttggattc ttttatcggg
    20821 gctagtcaag cgagcggctt ctttacccaa gccggtttct tttaagaaaa atggatgatc
    20881 gattccgtca acaagatttt tttgatccaa atcactagca aaaacggacg ctaatttttt
    20941 aggtatttga aaatcgaaag cacttcctgc agcattttct tttaccccta taggaatact
    21001 atctgttcgc aaaggagcat aagcttcgct attgagccaa agctcatgtt gatcgattga
    21061 ctgagaagca tctcccgtca agttgaagta aacatgattt gtcggattga ataaagtatc
    21121 ttggtcactg gtgccgcgag aggtcactcg ccagatatta tcttttgtca aagtatagcg
    21181 gatttccaca tccattgtac ctggaaaacc atgttctccg tcgggacttg tagtagtaaa
    21241 aatgacagaa gcttcatttt cgccattcaa aatgacataa gaccattttt ttaattcaaa
    21301 tccaggagca ccaccatgca gattatgtcc agtttttgga tctgtggcta attggtaagt
    21361 ttttccattg agagaaaagt ggccattttc gattctccct gcggtgcgac cgatagaagc
    21421 accgatatac ggatcttttt cgatatattc ttcagcggtg tcaaatccca aaactaattc
    21481 tcgatcattt gattttaaac tgacgattct agcacctaaa tcagagatag aaagtgctag
    21541 cttgtttttg ttctccaagg ttatcaagtg ataaccttgg ccaaaatctt tttctgtaat
    21601 cttcattttc aagatccttt cttaatggga aggaactact gcatcgtgaa gttcatcttc
    21661 cattgtatca gctggagcca atttgttttc caatttctca acgatttgtg catgtttttt
    21721 ctctgtcaat gtaatcttcc aagcaaaaat aatagcggag aaaatcatca aagcagcagg
    21781 agcatagaat gcataagctt tgaaagtcgt tacgccatga gcagtgatat cagcagcagt
    21841 agcattacca gtcatcccag aagcgctagc gacaaaccct acgatcccgt tagacaaagc
    21901 accagcgatt ttatctagta aagggcgaat tgatagagta acagcttcgt tacggatacc
    21961 atttttccat tgaccatatt caacagagtc tgtgatcgtc atcaaagcgg ataagaaaat
    22021 caattgttga gggaagtaga acaatactag cccgatgata acgattggca aacttgaacc
    22081 tgcgatcgta aagaaaaagt aagaagaaat catcattgcg atccctccaa ggtaaacgta
    22141 gcgacgagag atgacgcgtg taagcattgg gaatagtgga acagctacta aaccaagtaa
    22201 ggcagcaact agaccaacga tccaaaattc agttggttgg ccaagtacat atttgaagaa
    22261 gtagaataat acggcagttg ttgctacatt ggcaatcgca taagcgatat aagcaagggc
    22321 aagccacatc aactgatcat tttgagcaac tgctttgaac acatctttga tcccagcttt
    22381 ttcattttgc tgacggatcg cagtatcgac ttcttttgtg cccaaggcag tgataatcgc
    22441 agtaccgcct gaaataacag cagcaatcac accgaaccag aaccagcctg aagcgccttc
    22501 aatatgttta tgagtaacaa gataggtaaa gaaagtaacg atagggacaa cgactagagt
    22561 agtaccattt gcacctagag aagagccgaa acgagcaaac gtagcaaatt tttcacgttc
    22621 ctttgtttcc gtactcaaag ttggaatcat tgaccaaaaa gcgatatcct taaatgaata
    22681 aaaactatcc aataagatga aaacgattgc aaataatatg ataaataaag tcgtattatg
    22741 agtagctaag ccaaagaaat tggtaaagat tgctaccaag agtacggaac taacaacgcc
    22801 gccgataact aaccatggtt tgaatttacc gtattttgta cgagtattat cgatgatgcc
    22861 accaatcaaa ggatcaaaaa taatttcaac taagcggata cctacaacta agcttgtaac
    22921 tgtagcaatc atttttgcat tcgttgcctt atctgttcct gcgaacatct ggcttgtcac
    22981 gaataccata aaataagtgc ttaatgtaac atagaatgtg tcgtgtccaa aagcgcctaa
    23041 agcgtaggac acacgttgga tcattttgct tttcataatc tgtattcctc tcctgttcaa
    23101 tatataatac taagtaaacc ggttacaacg aataacaaag atcttttaat gacttaaact
    23161 taacaagaaa tcgcttcctt tttaatatat ttctgtgact tttatttaac tttttgttgc
    23221 attatactaa aggagtaagg agggatagac gtggagcaat cgattttttt aaaaaaggaa
    23281 catcaacaag tagaaaaaga attgtatttt gatttttgtg gtttctctaa aacgatgccg
    23341 tttcattctt ttggtcctgc tattcgcaat gattacatcc tgcatgtcgt gatggaaggc
    23401 aaaggatctt atcatgtgaa aaatcaacaa ttccaattga aaaaaggtga tctgttcttg
    23461 attcgtccag gagactcgac gttttactta tcagatggag aagatccgtg gatgtattgc
    23521 tggctttcat ttggtgggag tgctgcagaa gaaatcattc ggcagtcgct tttcagagaa
    23581 gaccaataca cgatggtctc ttctgacata caaaaatata tcgacattat tttagaatgc
    23641 atgaaagata cggaagaaac cttggaaaat gaattacagt taacagcatt aacctaccgt
    23701 tttttatcag tccttttaaa agatggcgga aaggtcagcg gtggtgtaca aaaggttcat
    23761 tcctctttag ctgttgaaac agtcaaatac atcgaggcac attatgctga aaaattgtct
    23821 gttgaggaaa tcgcccagca tttagcggtc aatcggagcc atttgtcgcg agtattcaaa
    23881 aatcatatgg gtaccagtat caaggaatat ttgatcgggg tacggatcaa tcgaggggcg
    23941 tttcttcttt ctctgacggg tgaatccgtg gaaagtattg cctatcaagt agggttcaat
    24001 agtctagtcg tatttagccg aatgttcaaa aagagtaccg gagaaacggc tacgaactat
    24061 cgtaaacgta tgaaaaacga aaattttaag aatattgctt tggatacctt gaaaaaacag
    24121 ttggaaaaac aaaccgcagt ttcttggaca acctgattag tctggttaaa atgacaagca
    24181 tcggcaaaag aaacttgtgg cggttatgct acactgtaaa gcgaagacta aaaaggagga
    24241 aaagggatgc gtacacttat catcattagc catccaacga tcgaagaatc tttaaatcag
    24301 caatttttca aagaagcttc tgctggttta ccagctactt ggcatcattt ggaatcaagt
    24361 tatccagacg gaaaaataga tatagagact gaactacagc tcttggccca gcatgatcgg
    24421 atcattttcc agtttccgtt ttactggtat agttctcctg ctcatttaaa aatttggcaa
    24481 gataatgtct tggaacgagc agcacatgta ttaaaaggga aagaactagg aatcgtattg
    24541 acaacaggtg tggcggaaaa agaataccaa gctggaggaa aagaagaata tacgatttcc
    24601 gaatttttga ggccgtatca gcgaatcgca aataaattcc atatgaccta tcttccgcca
    24661 ttcgttttag cacaatttat gtatttatcc caggaaaaac gttgggagaa gctaattgct
    24721 tatcagcagt atctctcatt ggaaggcaaa ccatcgctga ctcagcggat tgactggttt
    24781 attcaaagag tacaggaaaa tcagaaaatg caggaagaag aatctgaaaa acaagcatat
    24841 atcgtagaag cactgactga ggcaaaagag cagatcgaag acttgacctt tacactgcaa
    24901 gaaatgaaag gaacttcatt atgacaaatg attggctgat ccaacaatta gatgaattaa
    24961 gcgaaaaatc agaatatcaa gaccgtgcgc tcttatatga aataaagaaa ctgatggctg
    25021 agatagacga acgaacaatg cagctgcaag gagaaatcga cggacaatct tggaatcatc
    25081 tcaactggtg aaattttctt ataaataaca aagataaaaa tgtgcacagc gacaattatt
    25141 tgctacaata agaatatgaa gcgtttgata acgctattat acataaaaat tggtggtgaa
    25201 aaaaatgtat gatgaaaatg agtggggaga aaatgttcgg atgacccgaa gaagacagca
    25261 aaaacaggca gctcgtaagc ggatgatcat gattggggca gcagttgccg tgtttttgat
    25321 catagcagga ggagttgcgg gtctatattt tcatggaaaa aattcgtcag aagcgaaaag
    25381 tacgaccata acggaaacaa cgacaaagaa aacagagacg aaacaaacaa gtgagtcatc
    25441 aactaaaaca aaagagacac aaacttctga agaaaaaaca gcaaatggtt tgccagagca
    25501 acttcaagca atcgtaaatg attattcgaa aaagatcgaa gaaaagacgc ctgcattgat
    25561 tgaagaatac cagacagaaa ttcagggaaa tcaagaggga atagctggtt tatcagcagt
    25621 tgctaaccaa aaagcacgtg agcttcaagc aatctccgac gaagggatca gaaaattacg
    25681 tgcagcgtat caggcagcag aaaataaaga cggtgtagac ttagatactt tgatcaatca
    25741 attatcggct aattatacga atcatgtcgc taagatttcc gatatttatt tgcagactag
    25801 cgcctctctt caagcagagt caacaagcac acaagacaca acatcttctg attctgagac
    25861 agcagagtcg gtagaagctt ctgaaatgca agctcgaaca acacaagaca caacagattc
    25921 ctctgaaact aataccaaca gcgcgacttc acaggctgcg acaaccgtag ttttggaagg
    25981 agaaggtcca aatcaaatag ccgaacgaac tggcgtacca gtagaaacga tcttgtcgct
    26041 gaatgggatg agcatggaga attatttcct gactcccggt gagacgctaa aattgaattg
    26101 ataaaagcaa taaaaaagaa cgaagcttcc actaaggtga agcttcgttc tttttttatt
    26161 ttccggttct tttttcgcgt acaaagtaat ggatcgtcat gtaaagcaag aaaacgagtc
    26221 caataatcaa tgagattctc gtttcaggat tgataaacat gaaaatcaat acgatagcta
    26281 aaaaagcaag tgtcacataa ttcgtataag gagatagtgg cattttaaaa ggatgatcat
    26341 gcattttatc tgcgtgcttc tttttgaagc gaatctcgct gatcaaaatc actacccaag
    26401 gaaccatacc aggcaaaata cttgagctgt atacgaaaac gaaaatattt tctgactggt
    26461 tgatgaacat tggtagaaga taatttaaga tcagaccaat taagattcca cctgagattg
    26521 ctacgacagg ataaaaaggc gcaccgtgac gagatagttt cagaaatttt ttagacatct
    26581 ttccatttat ggcaagtgta tagaccatac gacttgcgct gaaaatccct gaattgcaac
    26641 ctgataatgc cgctgtaatc acgacaaaat taatcagtcc tgcagcaaat gtaatgccga
    26701 ctttagaaaa agtttgtaca aaaggtgaac caatctcact caattgattc cacggataga
    26761 tactcacaat gataaaaata gcaccaatat aaaaaatcaa gattcgaccg atagtggact
    26821 ggacagcttc aatgatcgtc tttttaggat tctctgcttc accagcagtc attccgatca
    26881 gttcgattcc ttgataggaa gcaacaacga tcgataatgc aaagaaaaat cctttgacgc
    26941 ctccagtgaa gaatccacca tttttccaaa gatttgaaat ccctactgcc tgaccatgat
    27001 taccaaatcc aaataagatt accccaagac ctgcaatgat catcaaaatg atcgttatga
    27061 ctttgatgat cgaaaaccaa aattctaatt cgccaaacgc cttgacagaa ataagatttg
    27121 ccagcataag aaacaagacg acaatgattc cgggaataat tgccggcaaa tcaggccacc
    27181 aataattcat ataagatcct acagcaatca cttcacttat tcctacgaca atatattgaa
    27241 aaatattact ccaagcggtc aagtagccag ccacaggatg gatgtactcg ctcgcgtaat
    27301 ttgcaaaaga accagtcgaa ggatcaacgt aaagcatttc gcctaaagca cgcataatca
    27361 aatacaaaaa gaatcctgca atcgcatagg ctcccatcac tgagggccct gtccaacgta
    27421 tggttgagga ggcacccata aacagaccga caccgatcgt tccacctagt gcgatcattt
    27481 ggatatgacg ggcactaaga cttcgagtta acttttcttc ttccatacaa aaactccttt
    27541 cgttgttgag tcaacaagaa aaaataatca aacgtttcaa cacatgtttt tcattattag
    27601 gttagcctta ttgaactctt gatgtgattc tacagaaaat tttcataaaa tgcgaggaaa
    27661 attcgaaaaa aactttttca tcattctact tgaaaatgct ttccatttta gttaaactat
    27721 atttaaataa aaagttaggt aaacctaaaa aggagtgtaa gatttggaac gtacggccat
    27781 tgtaattcaa gacctttcag ccgcttatca agggaaaact gttttgaaaa ctatcaactt
    27841 gacgataaat ccacaaaaaa ttaccggtat tatcggacca aacggtgcag gaaaatcaac
    27901 atttatgaaa tctttgctag agttgatacc agctactact ggtaaagtca ccttttctgg
    27961 acagtcggta aactcagtcc gtaaaaaaat agcctacgtg gaacagcgaa gtgaattgga
    28021 tctttccttt ccaattgatg tattaggcgt tgtactttta ggaacatatc catctttacg
    28081 aattggacaa agacctggga aaaaagaaaa agaacgtgca agacaagctt tgaaaaaagt
    28141 agggatggaa gaatatgcca aaagacagat cagcgaacta tcgggtggac agcttcagag
    28201 agtttttatt gcaagagctc tagcccaagg agcagaatgg atctttttag atgaaccatt
    28261 cgtagggatt gatgcgttaa gtgaacgaaa gatctttgac atcttgcagg aattgaagaa
    28321 ttcaggaaaa acgattttga tcgtccatca tgatcttcat aaagtagaag aatatttcga
    28381 tgaggttatt cttttaaata aacagctgat cgcttccggt ccagtacaag aagtctttac
    28441 atcgaagaac cttcaattgg cctatggtga gatcatcaga catttagtaa aaggaggaga
    28501 gaaaaaatga tccaatcctt tattgatgga ttaatgaatt atcaattcct gcaatacgct
    28561 ttggtcactt ccatgcttgt aggacttgct tctggagtca tcggttcttt tattatttta
    28621 cgagggatgt ctttgatggg agacgctatc tctcacgcag ttcttccagg tgtagccatt
    28681 tcttatatgt ttggttttag ttacatcttt ggagccactg cttttgggat gctaacagca
    28741 gcagtgattg gtttcgtgac tcagcggagc cgattgaaaa atgatacagc gattggaatt
    28801 gtctttagct ccttttttgc attagggatt atcttgattt catatgccca gagtgcgaca
    28861 gatttgtatc atattttatt tgggaatgtc ttagctgtta gggaaagcga tttgttgctg
    28921 acagcactcg ttagtggaat cgtcttgata tttgtctttt tcttttataa agaattgaag
    28981 atcacatcat tcgatccgac aatggcaaaa gcttatgggc tgaacacatc gctgatccat
    29041 tatcttttga tgttcttttt gacattagtg gctgtagtca gtttacagac agtaggaaca
    29101 atcttggtga ttgccatgtt gatcacacca gccgccacgg cttacttgct aacgaaccat
    29161 ttactgaaaa tgatcattac agctgcagga atcggtatgc taagtgcagt tgtcggtgtg
    29221 tttttcagtt atagttacaa ttggccatca ggagctacga tcgtgttagc atgtaccgca
    29281 ttttttatcc ttgctttttt attttctcca acaaaaggaa ttttatttca gaaaggaagc
    29341 agatagatga aaacaaaaaa atcactcttt ttaatcttgg cagttagttt cttggtatta
    29401 gccggttgcg gaaagcaggc ttctgaccaa gcggatgaag gaagtaaaga aaagttgtca
    29461 gtcgtggcta ccaattcgat cttggcggac atggcaaaag aggtcggaac agatcaaata
    29521 gatatccaca gtatcgtacc agtcggaaca gatccgcatg aatatgaagt actaccagaa
    29581 gacatcaaaa aggcaagcga tgcagatgtt attttataca acggtttgaa tcttgaaaca
    29641 ggtaacagct ggttcgataa cttgatggaa acggctaaaa aagaagaagg aaaagattat
    29701 tttgcagtta gcaaaaatgt agaacctctt tatttaacta gcggtgaaga acatacaaaa
    29761 gcagatcccc atgcttggct agacctatct aacggaataa aatatgtgga ggaaatcgca
    29821 cgtatattct ctgaaaaaga tgcagaaaat gcgacactct ataaaaaaaa tgcagaagca
    29881 tatgtggaaa aactaaaaga attagataca caagcgaagg aaagttttgc ttctatcgaa
    29941 gagaacaaaa aattattagt aacaagtgaa ggggcattca agtatttttc acgagcatat
    30001 gatttgccag cagcttatat atgggagatc aatacagaaa gtcaaggtac gcctgatcaa
    30061 atgaaagcga tcattgatca gataagagca aaagaagtac cggttttatt cgtggaaacc
    30121 agtgtagact caagaagcat ggaacgggta gcaaaagaaa cgggtttgaa aatctatgat
    30181 aaactgttca ctgattccat agcaaaagaa ggagaacaag gagattctta ctatcagatg
    30241 atgaaatgga atattgaaac gatccatgaa ggtttgtcac aaacaaaaga aagctgaaaa
    30301 gtgcaaggtc tagcttgagg agcagacctt gcatttttta taagagatat gaaaaaaaat
    30361 tatgctgttt ttcgtcgtac agataccttt tttgaagaaa ttccgttata gttagagtaa
    30421 ggaaggaagg tgtggctatg aattttacac gtaaagataa atatacgatt aaagaagaag
    30481 tctttcgatt gatcagccag caggaaaata ccgacaagga tcaagacctt caatttcttg
    30541 aagcagaatt agctaaatta ttcatagcaa atcaaaatga gacaacagaa ccagatgaag
    30601 agctgtacat aggcaaatgg ttgtttgatg aatttcagcc tttagatgaa aataccggta
    30661 tcggttattt tgtgttcctt tctccagtcg gcttcacacc tggcgtatta gttaaacaaa
    30721 acgacgaaat aaccgtcaaa gcatggcctg cattattttc agtcatcaaa gcagaaggag
    30781 taatcaaaaa gatccagtcg gcctattata aaagtcaatt tattgccaac agcaaattaa
    30841 tgaatatgtt ttataatcgt ctagtagaaa ttgctaggcc tgggaaaatc ccgcaaacat
    30901 tcacgtacac acagcaaaaa aatctctatg aagaagtatt gtattcccgc gatctgtctg
    30961 agttttcgcc atgggaagag gtaaggctcc atgaactgtt aagtacaaaa ccagtttcat
    31021 tgaatttttt taatgcatta gataacaaaa gaaaattaat ttcaacaaat tattgggaag
    31081 tatttgctgc aagaaaaaaa tggatggaag aaaagaagat tgccgatcag cagatccagt
    31141 ttgtccagct gaaaaaagaa aaactatatt atgggttata cagccgttaa cagcaagcga
    31201 ggaagaccat gacattaaaa gaagaaatca tacaagaaag caaacgatta ggaattgaca
    31261 aaatcgggtt caccacggcg gaaccttttg attcattaaa agaatcgttg gaagaacaaa
    31321 aagcagctgg ccatacttct ggctttgaac atccaaatat cgatgagcgt ctttatccag
    31381 aaatgacatt tgatcagccg aaatcgatca tttccattgc acttgcgtat cctactagga
    31441 tccatgaaga agtgcctagg gatgaaaaac gcgggcagtt tgctcgtgct tcttggggga
    31501 tcgattacca tgatatactc agagaccgtt tgaaccgatt gatcgaattc atccaaacaa
    31561 gagcagaaaa atggcaaaaa gaagaggaat ggcgtctagc tccgcaagta gatactggag
    31621 aactgatcga tgtcgctgta gcacagcgag ctggtttagg atttattgga cgaaacggat
    31681 tgttgatcac tgaagagttc ggttcatttg tctatcttgg tgagatcgtt actaatattg
    31741 tatttgagcc tgataaacca ggagaatttg gttgcggtga ctgtatgcgc tgcgtgactg
    31801 cttgtccgac aaaagcattg cttggtgacg gaagaatgaa tgcccagcga tgtttgtctt
    31861 atcaaactca gacaaaaggg atgatgccgg aagaatatcg taaaaaaatg cgaaatgtca
    31921 tttatggatg tgatatctgc cagttagtct gcccttacaa tcaaggaaaa gattttcatt
    31981 ttcatgaaga gatggaacca aaagtagacg aaatatatcc aaaattaaag ccaatgcttt
    32041 cgatgtctaa taaagacttc aaacagcaat ttgggcattt agctggttca tggcgtggga
    32101 agaaaccttt gcagagaaac gcattgattg ctttagctaa tctaggtgat cgaacggcat
    32161 tgcctgagat ttcagaatgt ttaacagatg tccgccctgt catcagagga acagcagctt
    32221 gggctattgg acgtctagga acaaaagaac cggaaaaatg gcttgatgta ttagatgaaa
    32281 tattagctaa agaaacagaa gaagtcgtta taaacgaatt ggaacatgcc cagaaattat
    32341 tgcggagaaa gttaaaataa aaaaatagtt aacattattc aaagtttttg tccagtaatt
    32401 gttgtaacaa ttactggttt ttttgtattt tttacatttt tttaagagaa tttgataaaa
    32461 atcaaaaatt tttttgcttt tttttatgga ttattgcagt gaatgttaca gaatctgcgt
    32521 ttttcattca aaaataaaaa aacatagaaa aacgattact ttgcttatac aacaaggctt
    32581 ttatggtttt gtatcaaaaa aatatttgtt t
//
LOCUS       DACDIP010000025        32535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDIP010000025 DACDIP010000000
VERSION     DACDIP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.025"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..507
                     /locus_tag="IV257_001368"
     CDS             133..507
                     /locus_tag="IV257_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8814508.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IV257_001369"
     CDS             complement(621..1250)
                     /locus_tag="IV257_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8814509.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IV257_001370"
     tRNA            complement(1384..1456)
                     /locus_tag="IV257_001370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IV257_001371"
     tRNA            1670..1743
                     /locus_tag="IV257_001371"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
     gene            1698..1847
                     /locus_tag="IV257_001372"
     CDS             1698..1847
                     /locus_tag="IV257_001372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814510.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2340..3668
                     /locus_tag="IV257_001373"
     CDS             2340..3668
                     /locus_tag="IV257_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8814511.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3870..4703
                     /locus_tag="IV257_001374"
     CDS             3870..4703
                     /locus_tag="IV257_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8814512.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4719..5456
                     /locus_tag="IV257_001375"
     CDS             4719..5456
                     /locus_tag="IV257_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8814513.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5611..6579)
                     /locus_tag="IV257_001376"
     CDS             complement(5611..6579)
                     /locus_tag="IV257_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8814514.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6811..7644
                     /locus_tag="IV257_001377"
     CDS             6811..7644
                     /locus_tag="IV257_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8814515.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7678..8517
                     /locus_tag="IV257_001378"
     CDS             7678..8517
                     /locus_tag="IV257_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8814516.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8543..9103
                     /locus_tag="IV257_001379"
     CDS             8543..9103
                     /locus_tag="IV257_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8814517.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9302..11023
                     /locus_tag="IV257_001380"
     CDS             9302..11023
                     /locus_tag="IV257_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8814518.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11188..12330
                     /locus_tag="IV257_001381"
     CDS             11188..12330
                     /locus_tag="IV257_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8814519.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12346..13557
                     /gene="thiI"
                     /locus_tag="IV257_001382"
     CDS             12346..13557
                     /gene="thiI"
                     /locus_tag="IV257_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8814520.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14130..14777
                     /locus_tag="IV257_001383"
     CDS             14130..14777
                     /locus_tag="IV257_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8814521.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15170..17815
                     /locus_tag="IV257_001384"
     CDS             15170..17815
                     /locus_tag="IV257_001384"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8814522.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18125..19438
                     /locus_tag="IV257_001385"
     CDS             18125..19438
                     /locus_tag="IV257_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8814523.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19428..20081
                     /locus_tag="IV257_001386"
     CDS             19428..20081
                     /locus_tag="IV257_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8814524.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20136..20828
                     /locus_tag="IV257_001387"
     CDS             20136..20828
                     /locus_tag="IV257_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8814525.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20978..21178
                     /locus_tag="IV257_001388"
     CDS             20978..21178
                     /locus_tag="IV257_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8814526.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21432..23111)
                     /locus_tag="IV257_001389"
     CDS             complement(21432..23111)
                     /locus_tag="IV257_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8814527.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23113..23325)
                     /locus_tag="IV257_001390"
     CDS             complement(23113..23325)
                     /locus_tag="IV257_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8814528.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23718..25448
                     /gene="efrA"
                     /locus_tag="IV257_001391"
     CDS             23718..25448
                     /gene="efrA"
                     /locus_tag="IV257_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8814529.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25445..27205
                     /gene="efrB"
                     /locus_tag="IV257_001392"
     CDS             25445..27205
                     /gene="efrB"
                     /locus_tag="IV257_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8814530.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27300..27497
                     /locus_tag="IV257_001393"
     CDS             27300..27497
                     /locus_tag="IV257_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814531.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27650..28135
                     /locus_tag="IV257_001394"
     CDS             27650..28135
                     /locus_tag="IV257_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8814532.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28239..28832)
                     /gene="clpP"
                     /locus_tag="IV257_001395"
     CDS             complement(28239..28832)
                     /gene="clpP"
                     /locus_tag="IV257_001395"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8814533.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29012..29539
                     /locus_tag="IV257_001396"
     CDS             29012..29539
                     /locus_tag="IV257_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8814534.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29630..30367
                     /locus_tag="IV257_001397"
     CDS             29630..30367
                     /locus_tag="IV257_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8814535.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30371..31288
                     /gene="miaA"
                     /locus_tag="IV257_001398"
     CDS             30371..31288
                     /gene="miaA"
                     /locus_tag="IV257_001398"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8814536.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31290..32519
                     /gene="hflX"
                     /locus_tag="IV257_001399"
     CDS             31290..32519
                     /gene="hflX"
                     /locus_tag="IV257_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8814537.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10771 a         5522 c         6876 g         9366 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc tttttctttt
     1801 attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga
     1861 gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac gaaataaatc
     1921 agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac attcccaaag
     1981 ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc aatcaggttt
     2041 ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat gagtattttg
     2101 aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca ggccattttc
     2161 gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta tgacattttt
     2221 cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag aagagattgt
     2281 atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag gatgattgga
     2341 tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa gcaaatatca
     2401 aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct gctgaaaatg
     2461 tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg gggaaagtgg
     2521 ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca ttccgtcata
     2581 agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac acttttttct
     2641 gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca ggaaaagagg
     2701 caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat ggcggccaat
     2761 gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg
     2821 aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa ttactgcgta
     2881 aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac gctattgaag
     2941 aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca ttaggaacac
     3001 ctccaaaaga attcgatttt gagtaccgcg atgaagaaaa gaactatcat cttgaccgag
     3061 gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat gattatgtta
     3121 gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca gtagagatgc
     3181 taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg gaaactttca
     3241 aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt tgtgatgtag
     3301 gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca atggatgatc
     3361 tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga gaaagtctga
     3421 tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca acaaaatgga
     3481 aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg
     3541 cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg acgaaagagc
     3601 aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca atgggtgctt
     3661 tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag ctggcttaag
     3721 ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt gctataattc
     3781 taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga tgacgaagta
     3841 gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta tctgatctga
     3901 aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc tatcgtataa
     3961 aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg
     4021 aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg gatacgtata
     4081 tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag ccgggaaaag
     4141 gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca gcgccacaat
     4201 ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct tttgaagaag
     4261 attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg gcagaagaaa
     4321 cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga
     4381 aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg tggagtggta
     4441 atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt tcttatggga
     4501 atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg gaatttttag
     4561 atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg ccgatttacc
     4621 agttatatta tcttttggca catttgaata tgtttggaga aagttacggt tcccaagtag
     4681 atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa
     4741 agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt
     4801 taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga ggcagtgaat
     4861 tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag attaatcgtc
     4921 tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac aataagtttt
     4981 tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc aaaatagaat
     5041 ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat gaagagatca
     5101 ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga
     5161 ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat agtaatcagc
     5221 atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat gactttttga
     5281 ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag tacgggaatg
     5341 agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa
     5401 tccgaatgca agaacagctg tattgtgaag caaatattat atggaaaaat aaataaaaaa
     5461 gaggttgtga cagaaggggg caacttcaag aaataagaag aaatttacga aaattgtttc
     5521 tcaaattttt gtaaattttc gacttagttt tcgaagaagc tatttttgtc acagcctctc
     5581 tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa tcaattcttc
     5641 acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt gactattaat
     5701 actgttacag aaaataatcc ccattttttc tagttctttt cccccgcctt cataatcata
     5761 aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga ttttttggac
     5821 aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac ctaaagcttc
     5881 gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc cttgcatccc
     5941 agcatatgct ttaacgatcg ggatagtccc atctacagcg gaaatgtcca gtcgctttgt
     6001 ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga tgggacccaa
     6061 tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga
     6121 atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt cacaacttgc
     6181 gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat tacttgaacg
     6241 cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc
     6301 agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac ctgatgcata
     6361 agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg
     6421 aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga tttctgcatc
     6481 taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt ctttcatggc
     6541 gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt ccttacgaat
     6601 aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg ctatcagtga
     6661 agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat ggaaaagact
     6721 caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca agaaagcagc
     6781 caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac atcgtttaaa
     6841 gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc agggtttcta
     6901 cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc agttgagaat
     6961 tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca aggaatcagc
     7021 ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc gcatttttta
     7081 gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt ccaattcccc
     7141 tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag aaagtggttt
     7201 gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga tgaatttctt
     7261 gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat cgatgagcca
     7321 cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa ctttgctctt
     7381 tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa
     7441 aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa
     7501 gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc tggtggtgat
     7561 gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga tggactcaat
     7621 ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc gaagtctatg
     7681 atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc aaaagcgttg
     7741 gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg cggtgtagca
     7801 acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt agatatgtat
     7861 gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga ccaccctttt
     7921 gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg acaaatcgtt
     7981 tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca atcgattttt
     8041 atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc
     8101 ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga gatattaaaa
     8161 gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa gttagaacat
     8221 cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat catcccaaaa
     8281 ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa
     8341 gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg tgtaggaatt
     8401 ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa
     8461 tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat ttattagtca
     8521 atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag tagaaacatt
     8581 ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag aaaatgaagc
     8641 tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag ctacttcacc
     8701 taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag ataaagcaga
     8761 agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac aagtagatgc
     8821 actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa tagtgcctga
     8881 tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc
     8941 tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc
     9001 tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa
     9061 gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt
     9121 ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa
     9181 aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt
     9241 atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata
     9301 aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt
     9361 ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa
     9421 acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat
     9481 gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat
     9541 atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga
     9601 aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca
     9661 aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga
     9721 acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt
     9781 cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt
     9841 gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc
     9901 ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa
     9961 aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag
    10021 tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga
    10081 agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga
    10141 agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga
    10201 tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc
    10261 agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac
    10321 atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga
    10381 agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat
    10441 tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga
    10501 aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc
    10561 gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa
    10621 acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg
    10681 tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg
    10741 tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt
    10801 agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca
    10861 cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta
    10921 tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa
    10981 acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa
    11041 accagcaaga gcaagacgga ttattgtctt gttcttgtct ttttttgtat atttgaaaat
    11101 tgaaacaagt gaaaatttca cctgctcttg aaagcacctt ggacaaccgt tataatagaa
    11161 aagaatttca aaaaagaggg gtttcttatg atatattttg ataacagtgc aacaacccca
    11221 atctttccgc aaagcttaga tgcatatgta aaaaccagtc agcggattat tgggaatcca
    11281 tcaagcttgc atgatttagg caatcaagct aaccggttgt tgcagcaagc aagaaagcag
    11341 atcgctgatt tgatccatgt agaaccagaa gaaatctatt ttacttcagg cggaacagaa
    11401 ggagataact gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg
    11461 atcatttcag gagtcgaaca tccggccgtc agcgagacag cagaacaatt aaaagaatta
    11521 ggaatcgaag taagtatcgc cccagtggat aaacgcggat ttgtacgtgt cgacgaattg
    11581 aaagaattaa taagaaaaga gacagtatta gtctctgtta tggctgtgaa taatgaagta
    11641 ggatcgattc agcccatcca ggaaatcagt gatcttttgg atgattttcc taaaattcat
    11701 ttccatgtag atgctgttca ggcgattgga aaagtaccat tctcgcaatg gttgacagaa
    11761 agagttgatt ttgcaacatt ttcagctcat aaattccatg gcccacgggg aactggtttc
    11821 atttattgga aaaagggtcg tcgtttagca ccattactga caggtggagg gcaagaaaga
    11881 aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg ctatggttaa agctttacgt
    11941 ttatcattag agataaagga acaaaaacct gagcagacac aacgcttaaa agtagctcta
    12001 atcaatgcgt tgaagacgta tgagaaagtg acgatttttt cagaaggaac tgactatgca
    12061 ccgcatattt tatgttttgc tctaaaagga attcgcggcg aagttctcgt ccatgcctta
    12121 gaagaaaaac aaatatttgt ttctactact agtgcctgct cctcacgtaa acatatggca
    12181 agcagtacat tgcacgcaat gcatgtacca aatgatctgg ctacatctgc tgttcgaatc
    12241 agcttagatg aaaacaattc gcttgcagaa gtcgagcaat ttatgattat tttcaagcag
    12301 ctttataaga aattttctgc aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa
    12361 attatggtcc gttatgggga actttcgaca aaaggaaaga atcgaaaaag ctttatcatg
    12421 caactagcac aaaatgtcag acaagctttg aaagactttc cagaaatcaa aatccatgct
    12481 gatcgggacc ggatgcatct tttattgaat ggcgcagacg ggcagctagt tattccaaaa
    12541 ctagcaaaaa tatttgggat ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc
    12601 gaagttttga aacaagctgt tcaagacatc atgaaagaaa tttatactgg gaaagaaaca
    12661 tttaaaatta tcgctaaaag aagcgatcac caatttgaat tgacaagtaa tgaattgaat
    12721 caaacattag gtaatgctgt ttttgatatt ttcccgcata tccaagttca aatgcgtcaa
    12781 ccagatattc cattacgtgt agagattcgg cgtgatggcg cttatctatc gtatgaaacg
    12841 atcaagggag ctggaggatt gcccgtagga acaagcggaa aaggaatgct tatgctttct
    12901 ggggggattg attctcctgt agcagggtac tttgccatga agcggggagt ggaaattgaa
    12961 gctgttcatt ttgctagtcc gccgtataca agtgaacaag cactgcaaaa agcaaaagat
    13021 ctaacagcca aactagcacc ttatgtggga acgatccatt ttatcgaagt gccatttacc
    13081 gagattcaag aagaaatcaa aaagtcagtg ccacaaggat attggatgac aattactcgt
    13141 cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt agtcattttg
    13201 aatggtgaat cattaggtca agtggcttca caaactttgc aaagtatggt ggctatcaat
    13261 gaagtaacga atacgccaat cattcggcct gtagcgacaa tggataaact tgaaatcatt
    13321 gaagtagcag aacagatcga tacatttaat ctttctatcc agccttttga agattgctgt
    13381 acgatttttg ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat
    13441 gaagagcgtt tagcaataga tgaaatgatc caacgagcac tagctggctt gaagatggaa
    13501 gagatacaag cagaatcgtc taatgaaaaa actgaagaat tttcagaatt gctataagag
    13561 gttatgaaaa tagtgttttg accagagtat tagagaaaca aaacgaaaaa atagctccat
    13621 gtattttacc gtttttgaac taatgtcgaa gattagctga ttgaatacca tttatcaaga
    13681 agttatggaa aagccggttc ttttttacta actgaaaaat aaaattttat ttagagatca
    13741 agctacagtt gataaatgat ataaaaaaga gaatttccca tgaaatagcg aggttgtgac
    13801 agaagtgggc agcttcaaga aataagaagg aatttacgaa aattgctttt caaatttttg
    13861 tgaaatttcg gcttattttt cgaagaagtt acttctggaa caccgtgtat cagaggttgc
    13921 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt
    13981 tacgtttggt tgagcttatt accgcagatg caagcttttg aacaccgttt tatttgtctc
    14041 acaccctttt ccttcctact tgtaaccttg ctcccgtgtt agaaacatgt tacactaaat
    14101 atgtgaaatt attaacaagg agcgtcgtta tgcgagaaaa aacagataca ctgccaaaag
    14161 caactgcgaa aagattacct ttatatttac gctacttgaa gatgctggat gactcaggta
    14221 taagtagaat caaatcaaat gagttcagcg aaatcaccca aattccttct gcaacgatcc
    14281 gcagagactt ctcgcaatta ggcgagctag gcagaagcgg atatggctat gatgtccctt
    14341 ttcttatcga tgtattcaat aatatcctta atactaaaga agaaaaacga atcgcattag
    14401 taggatatgg aaatcttggc aaagcattga aacacaacaa ttttagaaga aatgaaaatc
    14461 taaatatcgt gtgcgtcttt gataatgatc cagcactgat caatcgagta attgatggag
    14521 aaatgatcta tccgattgat cgtttcgcgg agatagccaa agcaaaaaat gtgacagtag
    14581 caatctctac ggtaccaagt aaatattcac aatctgctat tgatgaaatt gtcaaaggaa
    14641 atgtaaccgc tatacttaat tttgcgcctg acagagtaac tgtcccagct tatgtcaatg
    14701 ttcaatacat tgatttgaca accgagttgc agacgcttat ttactttgat gagaattact
    14761 cagaagtttt ttcttaggaa aatttgagta ggcttattga tcttgcgaga actcgtcgta
    14821 attctttcaa aaatttttta gtactaaatg aagtggtttt ttcacgacca cttgttgact
    14881 aaaaaaaaaa accattgtag aatgaagcat aacgctgaac aagaggagta actggcacat
    14941 tcattcttag agagagaact atctgctgaa aggttctcaa tgacaaccag tgaaggtagc
    15001 ttgggagttt ctgtcttgaa tggagtagag atagacgagt cgtgatcgtt aactcacttg
    15061 agaggcaata atgattgatt attgcaattt aggtggtacc gcgtaaatca tacgtcctag
    15121 aatacgaaag tattttagga cgttttttag tttaaaggag gaaaacagca tgacggaaga
    15181 aaataaccta tcaacaaaat ataatccgca agaagttgaa gctggacgtt atcagcattg
    15241 gttagaacaa gatttgttta aaccgagcgg agataaaaaa gcaaaacctt attcaatcgt
    15301 aatcccgccc ccaaatgtta ctggaaaact tcatttaggg catgcttggg atacgacctt
    15361 gcaagatatg ctgattcgtc aaaagagaat gcaaggatat gatactttat ggctgcctgg
    15421 tatggaccat gcaggaatcg cgacacaagc aaaagtagaa gcgaagctta gagagcaagg
    15481 gatttctcgt tatgatctag gtcgtgaaaa gttcatcgat caagtgtggg aatggaaaga
    15541 agagtatgcg agccacatcc gcgaacagtg ggctaaaatg ggcttatctt tagattacag
    15601 tagggaacgt tttacattag atgatggatt atctgaagca gtacgtaaag tttttgttac
    15661 cttatacgaa aaaggtttga tttatcgtgg tgaatatatc atcaactggg atcctcaagc
    15721 acgaacagct ctatctgata tcgaagttat tcataaagat atcgaaggcg ccttttatca
    15781 tatgagctat gaactagcag atggatcagg cgtagtagaa attgcgacaa cccgtccaga
    15841 aacgatgttg ggagatacgg cgattgctgt tcatccagaa gacgagcgtt atcaagcgct
    15901 catcggcaaa aaagtgattc tacctttagt caataaacaa atcccaatca ttgcagatga
    15961 atatgtagat atggaattcg ggacaggtgt agtaaaaatc actccagcac atgatccaaa
    16021 cgactttgag gtaggtaatc gtcatgatct tccacgcgta aatgttatgg atgatgatgg
    16081 tacgatgaat gaactagcag gaaaatatgc tggaatggaa cgttttgctg ctcgtaaagc
    16141 aatcgtgcaa gatctgaaag aaatcggtcg attgattaaa atcgatacga tgatccacag
    16201 tgtgggtcat tccgagcgga caggtgtcgt tgttgaacct cggctgtcta ctcaatggtt
    16261 tgtaaaaatg gcgccgttag cgaaaaaagc cattgataac caagaaacag atcaagcagt
    16321 ggaattcttc cctccaagat tcaatcaaac gtttttacgt tggatggaaa atgtccatga
    16381 ttgggtaatc tcacgacaat tatggtgggg acatcaaatt cctgcatggt atcacaaaga
    16441 aacaggtgaa atgtatgtag ggatggaagc accagcggat agtgaaaact gggtacaaga
    16501 tcctgatgtt ttagacactt ggtttagttc agctttgtgg cctttctcaa caatgggctg
    16561 gccagatacg gaaagcgaag attataagcg atacttccct actagcactt tagtaaccgg
    16621 ttatgacatc attttcttct gggtaagcag aatgattttc caaagcttgg aatttactgg
    16681 cgaacgtcca tttgagaatg tcttgattca tggattgatt cgtgacgaac aagggcggaa
    16741 gatgagtaaa tcattaggta atggaatcga tccgatggaa gtaatcgaaa agtatggagc
    16801 agatgctctc cgctggttct tatcgaatgg ttctgcacca ggacaagatg tccgcttcag
    16861 ctatgaaaaa atggatgctt cttggaactt tatcaacaaa atctggaatg cttcacgttt
    16921 cgttatcatg aatatcgaag gaatgaccgt agaagagatc gatttatctg gagaaaaatc
    16981 agtggctgac cggtggatcc ttactcgttt gaatgaaacg atcgaacgag taactgagct
    17041 atttgatcgt tttgaatttg gtgaagcagg tcgacaactg tataacttta tctgggatga
    17101 tttttgtgac tggtacatcg aaatgagtaa agaagtactt tatggagatg atgctgtttc
    17161 aaaacaaacc acccgtagca tcttagtcca tacacttgat cagatcctac gtttattgca
    17221 tccaatcatg ccattcgtta cagaagaaat atgggaacat attcctcatc agggaaattc
    17281 tctagtagtg gcagaatatc ctgttgtacg tccagagttt gatgatgaaa cagcttctaa
    17341 agggatggaa gtattgaaag aattgattcg agccgttcga aatattcgtt cagaagtgaa
    17401 tacaccattg tctaaaccaa ttacgttgct aattaaaaca aacgatgcta aaatcgatca
    17461 atttttagtt gaaaatacaa gctatatcga acgcttctgt aatcccgaag agttgacgat
    17521 atcaagtgag atcactgcgc cagatctagc gatgtcagct gttctgacag gtgcagaaat
    17581 cttcttgccg ttagcaggat tgatcaatat tgaagaagaa atcaaacgat tagaaaaaga
    17641 actggaaaag tggacaagcg aagtaaaacg tgtccaagga aaactttcta atgaacgttt
    17701 tgtttctaac gcgccggaag aagtagtaga ggcagagcgt gcaaaagaaa aagattattt
    17761 agagaagcaa acggctgtca aagaacgaat cgaaagtttg cgtactattc aataagaata
    17821 gaaaaagaga gtgtgaaatc agctagctat tactaaaagc tagctgattg aacatcgtta
    17881 caggaaagag tttgagcctc actcttagct tcgattcata aggagatgtg acataaattt
    17941 tgtcgcatct cttttttagt ctactcttat tatgtaggag aaggagggag gtggatcatg
    18001 tatcaagagt caataaagta gttgataaaa tcgttcctct ctctgtttct tttctctttg
    18061 tcatactttt atttttctta tgtgttctat ttgttataat aaaagagaaa tgggggaatt
    18121 gttgatgacg attgaagaag caatagaatg gatccatagt cgattaccat ttggttcacg
    18181 tcctggcctt gaccgagtcg aagctttatt agaagtggta gggcatccag agagaaaagt
    18241 aaaaacgatc catatcgctg gaacaaacgg aaaaggttct actgtagctt atttgagaag
    18301 cctattagaa gaggctgggc tgacagtagg tacatttact tcaccttata tcgaaacgtt
    18361 caatgaacgg atcgcaatca atggcaaaag cattccagac aatgatttga tccagcttgt
    18421 aaaaaaatat cagccactga tcaaacaact tgatcaattt gaagcagtag ctggtattac
    18481 tgaattcgag acattaacag caatggcatt tgattatttt ttgatgaaga aagtggacgt
    18541 agcgattatc gaagttggct taggcggctt attggattct accaatgtag ctgtaccaat
    18601 gctgacggga ataacaacga ttggacttga tcataccgaa atacttggag atacaattga
    18661 agaaatcgct gcacaaaaag ctggaatcat caaacaaggc gttccagtag taacaggaaa
    18721 tattgcccca gaagctttta ctgtcatcca acaaacagct aaagcaaaaa atgcaccgct
    18781 ttatgtatac ggggaaaatt ataaaataaa ctatctgcat cctgatgaga tgtggggaga
    18841 agtatttgaa ttttttaatg aaataggaaa gatccctcaa ttaaccactc cgcttttagg
    18901 gcaacatcaa gctgaaaatg caggagtagc catcgaattg tattttgttt actgtcaatt
    18961 ggaaaagctg cctttccgag aaaaagatgt tttgaaggga ttgaagaaat ctcgatggcc
    19021 aggaagaatg gagcgtttga gtaacgagcc attagttgtt ttagatggtg cccataatac
    19081 gcatgcaatg aaccggcttg ttgagaattt gaaaaaagaa ttcaaaggat atcaaatcaa
    19141 cattttgttt tccgccttac gaactaaaaa tgttagagaa atgctagaac gattgcagca
    19201 agtaccaaat gtccatattt atttgacgac ctttgaacat ccaaaagcca tcgaattaag
    19261 tgaagtatca gatttagcaa gtgagagagt atcaatcgtt tcattgtggc aattcggatt
    19321 agcagatata ttggaaaaga tgacaaatga agacgtatta gttgtgaccg gttcacttta
    19381 tttcatttca gaagttcgcc atttattaat acaattagga ggagatcatg aagtataatg
    19441 cagtgatttt tgacatggat ggtttgctgt tcgacacaga gttagtgtat tacgaagcca
    19501 cacaagtagt agcagacaag atgggttttc cttatgataa agacctgtat ttaaaattct
    19561 taggtgtttc agatgaagaa gtatgggcga attatcatat gattttctcg gaatttggca
    19621 aaacaagtgt acaaagattc atagatgatt cataccagga aacgcttgaa cgtttttcgc
    19681 aaggagcggt acaaatgaag cccggtgtgt tggaactcct ctctttttta gaagaacatc
    19741 agattcccaa agttgtcgct tccagtaacc aacgagcagt cattgaactt ttattgaaga
    19801 agaacaatct gtttgaacga tttgagacga ttgtttctgc agaaaatgtt caacgagcaa
    19861 aaccagaccc agaaattttc ttgttagcta atcagaagct aggtacagcg aagaaagaaa
    19921 cattgatttt agaagattca caaaatggta ttttagcagc ttctgcggct gagatcccgg
    19981 tagtcatggt accagatctg cttccaccat cccaagaatt gatcaaaaaa acagctgggg
    20041 ttgtttcttc tttacatgaa atccctgcat tgttaaaata aaaatccatc tcttttcgta
    20101 tcttatttta gataagatag aaaggagatg tttttttggg aaaacgattg atcaaagaag
    20161 tacctactag ctctttacca agggaacgta tggaaattta tggcgcggaa gctttatcgg
    20221 atcaggagct tttggcaatc ttactgcgta ccggtcaaca cccttatagt gtgatgtcga
    20281 ttgctggaaa tctgttaaaa acgtttggag gactggcctc tttacgacag gctactttgc
    20341 atgagttaga agagattcga gggataggac gcgtgaaagc aatcgaaatc aaagccttga
    20401 tcgaattagg tcgacgaatt cagacggatc atgaaagagc ggctccaccg gttcgtacca
    20461 gctacgaatt ggcccagcaa ttgattctgg aaatgaaaga tcacaaacaa gaacatttgg
    20521 tttgtattta tttggacacg aagaatcaag tgatcttaaa aaaaacagtg ttcatcggtt
    20581 cgctgaatca gtcagtcgct catcctagag aaattttcca ttatgctgta cgttattgtg
    20641 cagcaaggat cgttttagcg cataatcatc cttctggtaa tgttattcca tcgcagcagg
    20701 atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc actgtactgg
    20761 accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga atgatggaag
    20821 agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata ttgtaatttt
    20881 gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc aatgttacaa
    20941 tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa cagtaaaatg
    21001 gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg acgtattcgt
    21061 tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc aagcagtgac
    21121 tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa aagcttaatt
    21181 ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa agcgagtaac
    21241 aaaaatacct gccacaagag acaatttctc ttgtggcagg tatttttttg tatgaaaaag
    21301 taaataaatt taagaggcta tgaaagagcc accttgtatc aaggaataag aacaaacaaa
    21361 aatggcttct catgtttttc atttgttttc tcttatattc aggacaaggt gctgatttca
    21421 taacctctta tttattcttc atctggtgtc attaccattg gcaaaatcat tggatgtctt
    21481 tctgtacgtt cgaataagaa aggctgaagc gcgccagtca tcgcttcttt caatttatat
    21541 tctgaacagt caggtccatt caaacattct cttaaagcgt ggaataagat tcgctgagct
    21601 tcattgatca tttctcctga ttctcgcatg tagacaaatc cacgagagag aatatctgga
    21661 ccagccaaga tttctttttt cttgatatcc actgtcgcga cagctaatac aagaccttct
    21721 tctgaaagca ggtgacggtc acgtaaaacg acattaccaa tatcaccgat cccgctacca
    21781 tcgatataca catctccagc cacaaaatgc ccagcaggac gcgcagaatc gctagttaac
    21841 gctaaaacat cgccattttc catgatgaag cagttttctg caggaacacc tacatcttgt
    21901 gctagagaag catgtacttt caacatgcgg aattctccgt ggacaggaat aaaatatttt
    21961 ggcttcatca aacgaagcat taatttttgt tcttcttgtc caccgtgacc ggaagtatgg
    22021 atgttattga tttttccgtg aatcacttct gcaccagctt cagacagcaa attgatcaaa
    22081 tgattgacac ttgttgtatt accaggaata ggtgaacttg aaaagatcac tgtatcgtct
    22141 ggctggattt ggatttgacg gtgagtacca ttcgcgatgc ggctcaacgc ggccatcgct
    22201 tctccttgag aacctgtaca taggatcatc gtttcattgg caggcaagcg attgatttcg
    22261 tgtgcatcta caaatgtgcc gtctggaact ttgatatatc ctaaacgttg accgttgacg
    22321 atggcatttt ccatgcttcg tccgaaaaca gcgattttac gaccagttgc cacagcggca
    22381 tctgctgctt gctgcaaacg gaagatgtta gaagcaaagc tggcaaaaat gatacgtcct
    22441 tcgatttttg aaaagatctt caaaatagaa tgcccaatcg ttttttctga tttagtaaat
    22501 gttggaatct cagcatttgt actatcagat aaaaggcaca agactccttc ttccccgata
    22561 cgagccattt tatgcaagtt tgctggttcg ccaacaggag tgaaatcgaa tttgaagtct
    22621 ccagtcgcta cgatatttcc agaaggtgtt ttcaccacga tccctaatgc atctgggata
    22681 ctatgggtcg ttctgaaaaa gctgacagaa gtctttctaa aacggatgat cgtatcttca
    22741 ttgatctcat ggagctctgc atctcgaagc aagccatgct catctaattt gtttgtgatc
    22801 aacgctaatg cgagaggacc agcatagata ggaatatttg cttgtttcag caagtagggt
    22861 attccgccga tgtggtcttc gtgcccgtga gtaatcacca aggcttttac tttgtgtaag
    22921 ttttgtatga tgtagctgta gtcgggaatg acatagtcaa ttccaagtaa atcgtcttcc
    22981 gggaatttga tcccggcatc aatcaaaatg atttcatctt ggaattgtac cccatacgta
    23041 tttttcccga tttcacctaa tccaccaata gcaaagacgc ctgtctcgtt atttttgatg
    23101 gaaagtttca tattaaaact ccactaatga gaaatcagca ttttcttttt cgtactctag
    23161 atgacttcct tctagaagtt gaacgaattc aatattgtat ggggtatttt cttccacacg
    23221 ttggcgagcg tctacatcgc tttttgcctc aatgtaaaga gattttgttt cttcccgttt
    23281 agggttgcgt acttttgttt cttgataata aactttatag atcattgttt tgtctccttt
    23341 atctgcgtaa agcagtgatt tagctagttg atttttttag acaacacaaa acgaggggac
    23401 aagccacctg gtttttcata tgctttgact agaatactgg attcgagtca aagagacaag
    23461 cctcgttttt gcttatctgt tcggatattt tttcaaccgt accttctgta taaaaaatat
    23521 acaacaatta attgttattc tagcataaaa cagcaaataa gtatagaaat caactagatt
    23581 gaaagaaaaa atcaatgttt atcatcccgc tataatcata atgacttttt tatgagagaa
    23641 gtgttctatt aataggctcg ttgtgctatc atttttagta atgaaaatgt aacaaaagat
    23701 taaaaggaga tgagaaagtg aaactaatgt ggcattatac catgcgttat aagaaattct
    23761 tgtttttcaa tttcatatgc gtatttggtt ttattttgat tgaattagga cttcctacga
    23821 ttttagcacg tatgatcgat gtaggaatcc gcaatgatga ttttgattat gtgaagcaac
    23881 aagggatatt tatgatcgtt attactgtaa tcggtattat tttgaatatc ttgttaggtt
    23941 attttggttc gaggattacg actaatatcg tagcagatat tcgagatgat ttgttcgaaa
    24001 agatccaaag ttattcgcat caggaatacg aaaatctagg cgtatcttca ttgatcactc
    24061 gtacaacaaa cgatgcttat cagatcatgt tgttcctgca aaatatttta cgtatcggtt
    24121 ttatggcacc aatgatgttc attgtcagtt tgtacatggt catgcgtaca agtgtgacac
    24181 tcagtttgta tgtagtaggt gcattacctt tactattatt ggcagtcgta gcgattgcta
    24241 aggtgtctga accgttatct aaaaagcaac aaaaaaactt agataaaatc aacagtatct
    24301 tgcgggaaaa tctttcagga ttgcgtgtga ttcgtgcatt cgtcaatgaa aagtttgaag
    24361 aaaaacgttt tgctgtagtc aatgagaatt atgcttctag ttcaaaaagc ttattccgtt
    24421 tgatggctgt tgcacaaccg ggtttttatt tcttatttaa tatcgtaatg gtattgatca
    24481 tctggagcgg ggcagtgcaa atcgatgcag gcgagcttcg tgtaggagat ttgattgcgt
    24541 ttatcgagta tatattccat gccttgttct cattcatgtt atttgctagt gtgttcatga
    24601 tgtatccaag agcggctgtt tcagctggac gtatccaaga agccctagat gccaaacctt
    24661 cgattgtaga agatccaaat ggtatatctg atacaaaaac taaagggtat atcgaatttc
    24721 aaaatgtaac atttgcttat ccaggccatt cgcaagagcc agtgatacga aatgtcagtt
    24781 ttacagcctc tccaggagag accgttgctt ttattggaag taccggtagc ggaaaatcta
    24841 cgttgatcca attgatccct cgcttttatg atgtgagtga aggaaagatc ttgatcgatg
    24901 gagtagacgt gagagattat cggctaagca agttgcgaga taagattggg tttattccgc
    24961 aaaaagcgct gctatttact ggaacgattg cagataattt gcgatacggg aaagaagatg
    25021 ctacgcaaga agaaatggaa cgtgcagctg agattgctca agcttcagaa ttcatttctc
    25081 gaaaaccaga tggatttaat gaacatttat cagaaggtgg ttccaatttt tcaggtggac
    25141 aaaaacaacg attggcaata gccagagcca tcatacgccg tccggaaatc tatatttttg
    25201 atgacagctt ttctgcatta gattatcaaa cggatgcgaa gttacgtgct cgtctgaaaa
    25261 aagaaacgac agaatctact gtattgatcg tagcacaacg tgtgggaacg atcatgcatg
    25321 cagataagat catcgtatta aatgaaggag aagtcgtcgg tatggggacg catcgggagt
    25381 tgttggaaaa atgcccgatt tattatgata ttgccgcttc tcaattatca aaggaggaat
    25441 tagcatgaaa aaaacctttt cttccttaaa acgtttagct cgatatatca gaccgtatcg
    25501 tgagactttt cttttagtga tcctatttac agttctgaca gtggctttca acgtggcttt
    25561 gccatatgtt acaggacttc ctactacaga aatcagccgt aatattgcca atggcgaatc
    25621 aatcaatttt tcttatatat tcaaatgtct catttggatc acagttgttg gaatagggta
    25681 ttgtatttcg caattacttt ctggtgtttt gatgacaaat gtcgttcaaa gtgccatgca
    25741 tgatttgaga agagacatag aagaaaaaat caatcgtctt ccggtttcat atttcgataa
    25801 aaaccagcaa ggaaatattt tgtctcgtgt gacgaatgat gtagatgcag tcagcggtgc
    25861 tttacaacaa gcttttattg gtatcgtaaa tgcgatattg ggaattacgt tggctgcagc
    25921 aatgatgttt tatatccagc cgatgatggc gctgatctct atgatcatga ttccaacatc
    25981 gatctggatc tcaaaacgag tgatcaatgc ttcgcagaaa tattttcaaa gcatgcagaa
    26041 ttctcttggt gagctaaacg gctacgtcca agaaaatatg acaggtttta gtgtattgaa
    26101 agtatatgga cgcgaaaaag aaacattgga aggattcaaa aaagttaatc acagcttgaa
    26161 atactttggg tttcgtgcag cattcatttc tggcttgatg atgcctctag ttcaattaac
    26221 tgcctatgct acttatattg gtatggctgt tctaggaagc ttttatgtta ttactggggc
    26281 gatcgttgtc ggtcaactac aagcattcat ccaatatatt tggcaaatca gtcagccaat
    26341 ggggaacgtg acacaattgt cttcagtttt gcaaagtgca tcagcggcaa cgaaacgtgt
    26401 attcgagatt ttagatgagc cagaagaaaa agaaaacaaa gtagatgctt cactgccaga
    26461 aacgatttta ggagatgtga cattcgatca tgtcgatttt gcatatgatc cgaagaaacc
    26521 attaatccaa gatttgaatt ttgaagtgaa agctggtcaa accgtagctg tggtcggtcc
    26581 gaccggtgcc ggcaagacga cattgatcaa tctattgatg cgtttttatg acgtagacaa
    26641 aggagcaata aggattgatg gaatcgacac caaatcaatg agtcgcagtg atgtgcgttc
    26701 gctatttggt atggtcttgc aagatgcatg gctttatcaa ggaacgattg cagataatat
    26761 ccgcttcgga aagcttgatg cgacagatta tgaagtagtt gatgctgcga aaacagcaaa
    26821 cgtagatcat tttatccgaa cgatgccaga tggatatgat atggccatca attctgaggg
    26881 agacaatgtg tctttaggac aaaaacagtt gctgacgatt gcccgagcag tggtctccga
    26941 tcctaaaatc ttgattttag atgaagcaac aagttcagta gacacccgtt tagaagcgtt
    27001 gattcaaaaa gcaatggatc gcgtgatgga gggacggaca agtttcgtca tcgctcaccg
    27061 tttatcgaca atccgtgatg cagatctgat tctagtgatg gatcaaggtc agatcattga
    27121 aaaaggaacg catgagagtt tattggcaca aggtggattt tatgaaaaac tgtataacag
    27181 ccaatttgct gaggaagcag aataagtcat ataagaataa aacagtttaa aacttatttt
    27241 ttgttctcta ttcttgataa tggtaaagta aagttatcaa gaaaagtgag gacacggaga
    27301 tggaacagtt tttaaatgaa aaaattactg aagccaaaag agaagttgat cgtttaacgc
    27361 acgtctgtca gacgaagcct tttgattcag ctgatttttt tgaaaatgag tttcaaatcg
    27421 aacgcttggc agcacaaata gaagtgtacg aattaatttt aaaaaaagta atagaagaaa
    27481 atatagatgt acaataaaaa caggtaaaac gtcagttacc agatactgta aatagacatc
    27541 tggcctgagc aggttttacc tgtttttttt cttatgaaat aaatatatgc tatgatacct
    27601 ttattcaata ggattagttt ctacaaaaaa tgaaaggaga aaaataaata tggaacggtt
    27661 tatagcatca aatgccacag tgattggtga tgtgacattg tcagaagatg tgacgatctg
    27721 gtatcaagct gttttacgcg gggacagtaa ttggataaaa ataggacaaa gaacgaatat
    27781 ccaagatggt acaatcatcc atgtcgatca tgatgcacct gtagatattg cggagaatgt
    27841 gacggtagga catcagtgca tgctacatgg ctgtacaatc gaaaaagggg cgctgatcgg
    27901 gatggggacc acgatattga atcatgcggt tattggtgag aatagtttga ttggtgctgg
    27961 atcattagta acagaaggaa aagttattcc tcctaatgtc ttggcttttg gtcgcccagc
    28021 taaagtgatc cgtcctttga caaaagaaga aatacaaaaa aacaaagaaa acatccagca
    28081 ttatgtcgag ataggtcgga tgcatgcgca aggaaaattc aaggagcaaa gataggcagt
    28141 agcatccata aaataaaaaa ggatggtcag tttgagttga ccatcctttt ttatttggtt
    28201 tagataagag agcttaaaaa agcttccttt gaaacagatt aatttaatga agaactgttt
    28261 tccatgactt catcgatcaa tccatatgct tttgcttgtt cagctgtcat atagttgtca
    28321 cgatctgtat ctttttcaat cacttcaagc ggttgtccag tgcgttcagc taaaattttg
    28381 tttaagcgtt cgcgtgtttg caaaatatgg cgtgccgcaa tttcgatttc agttgcttgc
    28441 ccttgagcgc cgccaagagg ttgatggatc atgatctcag catttggtaa agcaaatcgt
    28501 ttaccttttg taccagctgt cagcaagaag cttcccattg aagcagccat acccataaca
    28561 attgtttgga catctgcttt gacaaagttc attgtgtcgt aaatagccat acctgctgta
    28621 actgaaccac caggagaatt gatataaaga tagatatctt tttcagaatc ttgagcgtct
    28681 aagaaaagca attgggcgat gatcgaatta gcaagatcat ctgttacttg gccgcttaac
    28741 atgatgatac ggtctttcaa taaacgtgag tagatatcgt aagctctttc acctcgtgaa
    28801 gattgttcaa tgactgtagg aattaaattc ataatagagt tcctccttga tttcgtttag
    28861 aaatagtgta ccataattta gtttctaata cctaaaatgt agtatacatt tttggtcaaa
    28921 aaaggtcaaa taaaaaactt aggcaaatta gtcgcataaa cccgctttat ggtatgataa
    28981 gcagagaaac aaaacaagga ggaacaacag attgatagtt ggtattactg gccttatcgg
    29041 agtgctgaca ggtgctgtag ttgtctatat cggcatgata ctgcaaatgc gtcacttgga
    29101 aaataaagaa cagcgtagac gtgaattgga attgaaagtc aaagaaatcg agacgctgaa
    29161 tcaattaaat aaaaagatca acgagatctt gcaaaaacgg acaacgctga tggaagaata
    29221 tgtcagcttt gatgcatttg atgattgtta tatcacgatt gacgattttg cgtatctcca
    29281 atcattttca gcgcaaaata gtttttatct gccaaattat attttagacg agctatttaa
    29341 gaatatctcc catcgaaaag tgattttatc tccagatgaa acaatgaaga ttggtggata
    29401 tgcttataaa ggcggacgcg tgattttaga gaattttacg gatagtctga ctgaaatgat
    29461 caatgaaaaa aaagctcagt tgaagactat gacgcgccag ccgattggtt tcttctcgaa
    29521 agatgatgtt tggagctgat attttgaagc attgccgttg cacatgagag cgtggtaatg
    29581 ctttcttttt ttcttaatga tacaatagaa ctgaacggag tgaagaaaga tgttacaaat
    29641 ctatgcccat cgcggaagca gcggcaccca cccagaaaat acgcttcctg catttgcaga
    29701 agctgtacga gtaggtgcag atggtatcga actagatgtc catctaagca aagatggtta
    29761 tttgatcgtt atgcacgacg aagaagtgga ccggacgact aacggtaaag ggctgattcg
    29821 agaaaaaaca ttggaagaac tcaaaaaatt gaatgcaggg ggcaaattca gcaaagaatt
    29881 tccagcagct aaagttcctg aattgagaga agtgttagga ctgctcctcc aaaaaaatta
    29941 ccgagggatg ctaatgattg aaattaaaac tgatcagtat gaatatcccg gtatcgaaga
    30001 aaaattagcc gaaacattaa aaagtagaga ttggcctttt caacatgcat attgcagctt
    30061 tcatttggat tctttggaaa aaatcgcatt actggaaccg gagatacagc tggacttatt
    30121 gatgagtact tctgataaaa agatccaact agcagcagaa accccttata tagaaggagt
    30181 acatccaaaa aatacttggt tgttcgaaca tccagtccag gcagctgatt ttccaaaagc
    30241 aattcgtgca tggacgatta acgatgaaga agaaatggcc aaatgtcaag aactcaaaat
    30301 agatggaata ataactgatt ttccggagaa agctcttcgg tggaaacaat caacgaaaag
    30361 taggtaaaat atggaaaaag tattagtgat cgtcggtccg acagcagtcg gtaaaacagc
    30421 gctaagtgtg gaactcgcac aactatttgg aggagagatc atcagtggtg attcattgca
    30481 aatctataaa aatctggata tcggtacagc aaaaatcaaa gaagaagaga tgaataatgt
    30541 tccgcatcat ttgattgatg tgatcgaacc tactcaacgt tactcagccg ctgattttca
    30601 gcattcaggt cgacaattga ttaccgagat tgcgaaccga gggcacctac ctatcatagc
    30661 tggtggtacg ggtctctata tccaatccct tctatatgat tatcagttag gagcgacaga
    30721 agaagcgaat cctgagatta gaaaaaagta tgaaacgtta gctgaaacaa ttggttccca
    30781 agcattatgg aatcagttga aagaaaaaga cccggcagct gctgagaaaa tccactggaa
    30841 caatcaacgc aaagtgataa gagctctgga agttttagag acgacaggac atagtatcac
    30901 ggcacctaaa gaagagccaa agcaattgta tgattacctc atgattggat tagatacgaa
    30961 gcgtagtatt ctttatgaac gaatcaatca aagagtcgat ttgatgctag cagaaggttt
    31021 agtagcagaa gcgcgtaagg tgtatgagct tggagaagtc caggccagtc aaggtattgg
    31081 atataaagaa tttttccctt attttgaagg gaaagaaaca ttggaagaag caacagaaca
    31141 agtcaaacta cattcgagaa gatacgcgaa acgtcagttg acgtggtttc gtaaccgttt
    31201 ggatgcacag tggtttgatc ttataagtga gccaaatcaa aaaagtcaga ttgagcaaac
    31261 aatcaaaaaa tggctggagg cggaataaaa tggaagaaaa agtgatcatc gtaggggtag
    31321 aaaccgaagc aaaccagcgt tacttttcag agtcaatgga agagctagtt cagctgacga
    31381 ataccgcatc aggagaagtc gtattcacaa ttacacaaaa gagaccacaa gtggatcggc
    31441 aaacgattat cggtaaaggg aaattacaag aattagttca acaagcggat gcgcatgaag
    31501 cggatttgat catctttaat catgagctaa ctccgagaca gagccaactg attaccgatg
    31561 ctgttggtct tccggtaatt gatcgcgtgc agcttatctt agatattttt gcgatgcgtg
    31621 ctcgttcgaa agaagggaag ctgcaggttg aacttgctca gttagaatac ctgctgcctc
    31681 gtttagtagg gcaagggaaa acattatctc gtcttggcgg tgggatcgga acaagagggc
    31741 caggtgaaac aaagttagaa acagaccgcc gacatatacg gaataaaatt tttgctgtta
    31801 agaaagaatt gaaagaagta gaagctcatc gagagcgcaa tcgccaaaaa agaaaaaata
    31861 gcgagatttt tcagattgga cttattggct acacgaatgc cgggaagtca actatcttga
    31921 atctattgac acaagcggat acttattcga aagatcaatt gtttgcaacg ttagatccat
    31981 tgactaagag atggcgtttt gctgaaggat ttgagattac tgtaacggat accgtcgggt
    32041 tcatccaaga cctgcctacg caactgatcg atgctttcca ttctacatta gaagaaagtc
    32101 aaaatatgga tcttttgcta catgtagtcg atgctagttc tccagatcgt attttacagg
    32161 agcagactgt cttaaaattg atggatgaac tgaatatgaa agaaatgccg atactgactg
    32221 tttacaataa ggcagaccag atcgacccag cgatgttcac gcctacgcta ttcccaaatg
    32281 tattgatttc tactcagacg aaagaaggta agatggcgtt gatcgaagca atcaaacgtc
    32341 agctgatgga actaatgacc ccttatacac tttctgtgcc aagcaacgaa ggacagaaat
    32401 taagcgagtt gagaagacaa acaatggttt tggacgaagc gtacatggaa gaaacaaacg
    32461 aatatgaagt taaagggttt gcaaaaacca attcaaaatg gttaagaaaa aaccaataaa
    32521 attttatttt tttaa
//
LOCUS       DACDIP010000026        30408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDIP010000026 DACDIP010000000
VERSION     DACDIP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.026"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..794
                     /locus_tag="IV257_001400"
                     /pseudo
     CDS             1..794
                     /locus_tag="IV257_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            934..1860
                     /gene="cysK"
                     /locus_tag="IV257_001401"
     CDS             934..1860
                     /gene="cysK"
                     /locus_tag="IV257_001401"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8814538.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2096..2542
                     /locus_tag="IV257_001402"
     CDS             2096..2542
                     /locus_tag="IV257_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8814539.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2814..4166
                     /locus_tag="IV257_001403"
     CDS             2814..4166
                     /locus_tag="IV257_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8814540.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            4337..4624
                     /locus_tag="IV257_001404"
     CDS             4337..4624
                     /locus_tag="IV257_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814541.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4850..5293
                     /locus_tag="IV257_001405"
     CDS             4850..5293
                     /locus_tag="IV257_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8814542.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5299..6483)
                     /locus_tag="IV257_001406"
     CDS             complement(5299..6483)
                     /locus_tag="IV257_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8814543.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6751..7449
                     /locus_tag="IV257_001407"
     CDS             6751..7449
                     /locus_tag="IV257_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8814544.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7456..8133
                     /gene="nth"
                     /locus_tag="IV257_001408"
     CDS             7456..8133
                     /gene="nth"
                     /locus_tag="IV257_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8814545.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8259..8792)
                     /locus_tag="IV257_001409"
     CDS             complement(8259..8792)
                     /locus_tag="IV257_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814546.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9089..11470)
                     /locus_tag="IV257_001410"
     CDS             complement(9089..11470)
                     /locus_tag="IV257_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8814547.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11460..12107)
                     /gene="recU"
                     /locus_tag="IV257_001411"
     CDS             complement(11460..12107)
                     /gene="recU"
                     /locus_tag="IV257_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8814548.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12171..12710
                     /locus_tag="IV257_001412"
     CDS             12171..12710
                     /locus_tag="IV257_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8814549.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12800..13219
                     /gene="gpsB"
                     /locus_tag="IV257_001413"
     CDS             12800..13219
                     /gene="gpsB"
                     /locus_tag="IV257_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8814550.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13241..13608
                     /gene="rnpB"
                     /locus_tag="IV257_001414"
     ncRNA           13241..13608
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV257_001414"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13817..14983
                     /locus_tag="IV257_001415"
     CDS             13817..14983
                     /locus_tag="IV257_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8814551.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15029..16525
                     /locus_tag="IV257_001416"
     CDS             15029..16525
                     /locus_tag="IV257_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8814552.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16606..17289
                     /locus_tag="IV257_001417"
     CDS             16606..17289
                     /locus_tag="IV257_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8814553.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17934..19124
                     /locus_tag="IV257_001418"
     CDS             17934..19124
                     /locus_tag="IV257_001418"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8814554.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19226..20179
                     /locus_tag="IV257_001419"
     CDS             19226..20179
                     /locus_tag="IV257_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8814555.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20248..21597
                     /locus_tag="IV257_001420"
     CDS             20248..21597
                     /locus_tag="IV257_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8814556.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21780..22103)
                     /locus_tag="IV257_001421"
     CDS             complement(21780..22103)
                     /locus_tag="IV257_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814557.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22232..23395
                     /locus_tag="IV257_001422"
     CDS             22232..23395
                     /locus_tag="IV257_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814558.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23465..24814
                     /locus_tag="IV257_001423"
     CDS             23465..24814
                     /locus_tag="IV257_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8814559.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24859..25290)
                     /locus_tag="IV257_001424"
     CDS             complement(24859..25290)
                     /locus_tag="IV257_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8814560.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25678..28320
                     /gene="alaS"
                     /locus_tag="IV257_001425"
     CDS             25678..28320
                     /gene="alaS"
                     /locus_tag="IV257_001425"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8814561.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28520..29116
                     /locus_tag="IV257_001426"
     CDS             28520..29116
                     /locus_tag="IV257_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8814562.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29238..30386
                     /locus_tag="IV257_001427"
     CDS             29238..30386
                     /locus_tag="IV257_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8814563.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
BASE COUNT        10158 a         5250 c         6344 g         8656 t
ORIGIN      
        1 ggaacgagtc ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca
       61 gatgaacaag gatctcaaac agttgctgat cctcaagcgt ttatcgatca aatcggctgg
      121 tctgcgtcag aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg
      181 ttgttggaaa gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc
      241 ggtgtgaaag gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaacg
      301 gacaatgggt aacgatgaat gaaccattcc gaaaatattc ttcttattgg gaatccttcc
      361 aagaccatgc gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg
      421 gcgtgtggaa aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt
      481 atgctaccga tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa
      541 cccgttttga tacgccatca acaggttcgg ctaccacaag tacaacaaca acgactacta
      601 cgacgacttc aacgtcatca tcttcaacaa cagatgtaaa tacaaataat ggtcagacat
      661 atacagtcgt gtcaggtgat acgctatggg atattgcaca aaaatttggc atgtctgttg
      721 atgatctaat ggcgaaaaat ggcatgtcta tgtccagtta ttctttatta gttggtcaag
      781 aactaaacgt ttgatttaga taaaaaggca aagaaactac catatatggt atatctttgt
      841 cttttttttg ctagtcaaag acgaatcact cgccataaat gaacagattg tattatgata
      901 agtaagaaga gcaaagacaa ggaggaattg aagatgacag ctatctataa ctcagtcacc
      961 gaattgattg gcaaaacgcc aatcgtcaaa ttaaataaaa tcgtaccaga agattcagca
     1021 gatgtttttg taaagcttga gttttttaat ccaggaggaa gtgtaaaaga tcgtattgct
     1081 ttaagtatga tagaaaaagc agaacacgat ggtctactga aaccaggtga tactatcatt
     1141 gaacctactt ctggaaatac aggaatcgga ttgtcgatgg taggagttgc aaaaggatat
     1201 aaagtaatca tcgtgatgcc tgagacgatg agtatcgaaa gacgtttatt gatgaaaggg
     1261 tacggcgcag agttgatttt gactcctggt gcggatggta tttctggatc aatcagagaa
     1321 gcagaacgtt tggcaaaaga aaacggctac tttttaccat tacaatttga aaatgaagca
     1381 aatcctcttg tgcatgagaa gacgactggt cctgagatac atcaagcatt tggagtaaat
     1441 ggcttggacg cgtttgttgc aggtatcggt acaggaggaa cgatcactgg cgctggccga
     1501 gaattaaaac gtgtgtatcc gaaaatcgaa ttaatcgggg tagaaccagc agaatctgct
     1561 attttagaag ggaaagaagc gggacctcac aaaatccaag gaatcggtac aggttttgtt
     1621 cctaaaacac tggatacttc tgtgtatgat aaagtccttt ctattagtgg agacgaagcg
     1681 atggaaacag ctagagaagt tgggcgaaaa gaagggattt tagtaggcat ctcatctggc
     1741 gctgcaattg ctgccgcgct aaaagtcgca aaagaattag gaaaaggcaa gaaggtatta
     1801 gctgtggttc ccgacaatgg tgaacgttat ttatctacag ccctttacca agagatataa
     1861 ggcacttggt ttttctccag ataggtttat gtaaaaaact tttaaagatg aaaagaacac
     1921 acaaaaattt atgatgagaa aaattatttg aatagagacg taaaatagtg aacgcgactg
     1981 ttttacgtct tttttcatta aaatacaata aaattgaatt ttcttttcat gttaagtgga
     2041 ttttaaaatg taaaaatagt atgataagac gagaataaca acgtggggtg tttacatgga
     2101 aaataagtta gtcgaagagg ttatcgaaaa gttaaaaaaa gagaacattc gaatcacacc
     2161 tcaacggtac gcggttttag aatatttgat tgaacatcat tcccatccaa cggctgatga
     2221 gatttataaa gcacttgaac atcggtttcc aaatatgagt gttgcgactg tttacaataa
     2281 ccttcgctta tttacttcga ttggatttgt tcaagaaatg aagtacggcg atgcttctag
     2341 ccgatttgat ttcagttcaa agcgtcatta tcatgtaatt tgtgaaaact gcggtaagat
     2401 tgttgatttt cattacccag ggcttgaaga tatagaaatg gctgcgagca gactgactga
     2461 ttttgagatt aatgaacatc ggttggaatt gtatggactc tgtccggatt gtaaagagtc
     2521 agcaaaaaag gcgcaaatgt aaaaacagtt tctataagtg ttatacctaa aaaaagctag
     2581 ctgtgtaaaa aaacgctagc ttttttattt ttttgtcttt tattcacaat cttagatatc
     2641 aaagtgaata ttatcacaat aagaatcttg tagatattgg gttcttatgc ttgaaaagga
     2701 atgaaactac aaacaaacct ttgtgaattc acaagcatta aactgttgta tccgttttat
     2761 ttctgttata gtagttttgt cgaaggacaa aatgttaatc ggagggacca taaatgaaag
     2821 tagtagtaat tggctgtact catgcaggta ctgcagctgt aaaaagtatt ttaaaaaatc
     2881 atccagaagc agaagtgaca gtatatgaaa gaaatgacaa tatttctttc ttatcttgcg
     2941 gtatcgcact ttatgtcggc ggagtagtaa aggacccagc aggcttgttc tactcaaatc
     3001 ctgaagaatt atcttcatta ggagcaacag ttaaaatgga acacgatgta acagacgtcg
     3061 atacagaaaa taaaacagta acggcaaaag atctgaaaac aggagaaaca gaaacggttt
     3121 cttatgataa attagtgatg acaactggtt catggcctat tatcccaccg attagtggaa
     3181 tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca agcaaatgaa attattgcac
     3241 aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg atatatcggt attgaattag
     3301 tagaagcttt tgtcgaatct ggaaaacaag tgacattgat tgatggatta gatcgcatct
     3361 tgaataaata tctagataaa ccattcacag atatcttaga aaaagaattg acagaccgcg
     3421 gtgttactct tgcgctaggc gaaaatgtta ctgaatttat cacagatgaa gaaggaaaag
     3481 tgaaacaagt agctacacca acagatacat ttgatacaga tatggtcatt ttatgtgtgg
     3541 gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga tacactgcca aacggggcaa
     3601 tcaaagtgaa tgaatacatg cagacaagtg atccagacat ttttgcagct ggagactctg
     3661 cagtagtcaa ctacaatcca agtggcacgc aaaactatat tcctttggca acaaatgccg
     3721 ttcgtcaagg tttacttgtg ggaaataact tgacagatca taaaatggcg tatcgtggta
     3781 ctcaaggaac ttcaggactt tacctatttg gctggacgat cggttcgaca ggtgtgacca
     3841 aagaaagcgc aactttgaac gatctagaag tccaagcgac tgtatttgaa gataattatc
     3901 gtccagagtt catgccaact actgaaaaag taatgatgga gctagtctat gaaaaaggaa
     3961 cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga cattacacaa tcagcaaata
     4021 ccatgtcttt agctgttcaa aataaaatga ctgtagaaga tctcgcactt tcagatttct
     4081 tcttccaacc acattttgac cgcccatgga attacttgaa cctacttgca caagaagcgc
     4141 taggcgaaat cgaaactgca caataaagaa aaagggttgt gacacaattt tgtcacgacc
     4201 ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc ttcgaatttt ttgtcacaat
     4261 aagcagcaaa gataaagctg ttacataaaa gaacaagcag ctttatttga ttttgaaaag
     4321 aaaggtgaga aagaagatga ataaagaata ttttgtccaa caagatgcat ttttagaaga
     4381 ttataaaagt gcttcgaaga aattaacgat caatattgat gagttggcag atcaagtact
     4441 ctctcagttg aatgaaacgc aagaggaata ttacacgatg ccagcaaatg acgccaaaga
     4501 taatgtttct catcgttttc ctttcagtag acgcatctac tcagatgaaa atgacggcac
     4561 attgaataca gagtacacat atgaaggtca gccatatgtg ctagaaactg atgatgatct
     4621 ttaaagacag tttttgaaaa ggccgctcat ccttaagtga tgacaaaaaa tcatcacaat
     4681 taccgatgac tgtctcttta ctatctttta atcagaaaaa ggggttatga caagctttgt
     4741 caaccccttt ttttcttggt ttcagattgg gttttcctac aaaatatttt tatctttgtt
     4801 gaataggaaa caaaaaaaca atataatgaa agaaaaagaa ggtgtagaaa tggataaaag
     4861 acaactgcta atagaagaat atctggattt agctaatgag attattcatc gtaataaacc
     4921 agaaatggaa aaagaatttg caggatttac tctaaatgaa ctagaagtga ttgaacatat
     4981 cgggaaaatc cctgatccta acgtgacaaa attagcgaat gctagttaca tgactagagg
     5041 agccatcagt aagctaacaa aaaaactgat agctagaggg atcataaaca cttatcaaag
     5101 cgaagagaat aaaaaagaaa tctattttca attgacgcca atcggacaaa aaataaggaa
     5161 tagacacgag caactccatc aaaaatgggt acagcgagat gctactgtat ttgaaaatat
     5221 gtcaaaagaa gaatttgaca cagtatttcg ttttatcggt cgatacagag aatttatacg
     5281 aagtagcaaa tgaagctact attttttatt gtagtgataa taggcaaaaa tcataagcaa
     5341 aaatccgaat accgtcggaa gaaatggtgc tgcagcataa agctgtcctg cgattagcgg
     5401 gccgatgacg cgcgatagag attgtatact ttgggtaccg cccattacaa gaccttgctg
     5461 gttatctgag acagaattgg atagttggcc attgaataca ggagtaaaaa tagaatcacc
     5521 gaaaccataa cagataatcc caaataaaaa caaaatcggc agtagaagaa ttccagataa
     5581 tgtgatgaat agataggcga ggatttcact gaacatccct attcttgtga tctgctgttc
     5641 tgaaaatttt ttgagcaatc gaggcataat gaacaattgc gagacagtat ccaagattcc
     5701 aatcaaagag aagcttaaac caaccaaccc agccttccac tgaaaggcgt caatcgaaaa
     5761 ttgagaaaag atagattgaa gagagccagc tgccaaccaa acagcaaaac ctgctgtcaa
     5821 taaaggaata acggatgaaa tcttgaataa ttgttgtaat tggtgaaaag gtcttacatg
     5881 ggagaaactt aaatctactg aacgtttttt tattggaagg ctttctagca taaatgtgta
     5941 gccataaacg gcatttaaga aagtaaagag tgctccaata aatataggaa cagaattccc
     6001 caactcagct aatagacccc ctataatagg acccagcgtt gttcctatac cgactaaagc
     6061 acccatccat ccaaaaactt tggttctttc attaggttcc gtgatgtcgg caaagtaagc
     6121 atataaggtg gagatttctc ctgccgtcaa tccttcaatg atacgaccaa ggaacaacat
     6181 ccatatcgaa tttcctaaac caaaaagcaa atatccgata cttgatccta ataaactgat
     6241 aataaggatc ggatgacgcc caaaatgatc acttaagcta ccgagaatgg gggcagataa
     6301 gaaggcagct agagcataaa cagacatgag caaagtgata tagaaagctt gctgatgtat
     6361 ggagtgagaa taaggcagcg ttaaaaacgg aagtacagga ctgacgatag taaatcctaa
     6421 tccagtgaat aagacagaga cgtatccaaa aactttagaa tgtttatttg tgaaactgta
     6481 catgtaaaaa ctcctttatg caataatgtt aacttggaaa caaaaatagt gtatagtaat
     6541 tttgtttcct tgtcaacatt ctaaggaaca gcgaaatcta cttaatttat ttattccgaa
     6601 aagtgaagat acgcatcaaa aacatgaaca aacaaaaatc aagtagacga cattgtaaat
     6661 ctgaccgatc agtgaagtaa atagaaaaga aacctgttct tgcttcccat gtttttttta
     6721 cgtatactgt gattgagcgg aggagaaatc atgttgaatt tagaaaatta cttaagcgct
     6781 ggacaaacag tcatatctaa tcttttactt aaaaattatc ataggatcgg tctacaatct
     6841 gaagaatttt tatttgttct acagttgcat atggcacagc ttgaaggaga tactttccct
     6901 gatttgcaga tgattgctat ggatatggga atcaaacaag atcagatttt tcaaatactg
     6961 gatcgtctcg taacaaatgg atttatcaag atagaaacga cgattgtcaa cggtaaaaaa
     7021 gcagatcgat ataatcttta ccccatctat gatctattag gtgagtattt aaaaacacaa
     7081 gaaaaaaaat acgaagaaaa aacacaagaa aaagaaatcc agtcagtgta tcaattattt
     7141 gaacaagaat ttggacgccc gctttcttcg atcgagttcc agcggatcgg acaatggcta
     7201 gaagaagacc attatcagcc ggaaattttg aagctagcac tgcgggaagc cgtattaaat
     7261 caggcttata gttttaatta cgttgaccgc attttgctat cgtgggaaag aaaaaatcta
     7321 cgtacaaaac aacaagtgga agaagaccaa aaacgaagaa aacagcagct tcttcaaaga
     7381 gaagtagaac agtcagctca acaagaagaa ttgccgaaag tctcactgaa aaattggttg
     7441 gaggagtagc ggaatatgtt aagcaaacaa aaaacaatgg aagctctaga aacgatgtat
     7501 ggaatgtttc ccgaagcaca tggagaactc aagcacaata atccatttga gttattgatt
     7561 gcagtgatct taagtgccca ggcgacagat gtatcagtga ataaagcaac gccagatttg
     7621 tttgcctctt ttccgacacc cgatgcgtta gcagaagctt caatcgatga aatcattctt
     7681 aaaataaaaa cgattggttt gtatcgaaat aaagcaaaaa acatcaaagc gtgtgcacag
     7741 cagctgattg aacgtttcga tgggcaagtg ccaacgtcaa gagaagaatt gatgtctctt
     7801 cctggtgtag gcagaaagac agcaaatgtc gtattggggg atgcattcgg gataccagca
     7861 atcgcagtag atacccacgt ggaacgtgtc tcaaaacgcc tgaggatctg caaactagat
     7921 gctacggtca tggaagtaga agaaacactg atgcggaaag taccacaaga attatgggta
     7981 aagacacatc atacactgat tttttttggt agatatcatt gcacggcaag aaatcccaag
     8041 tgcgaggtat gtcctttgct atcaatatgt caagatggga aaaatcggat gcgtctaaaa
     8101 gaaaaggcgc taaaaaagaa accacgcctg tagacgtggt ttctttaatt gcaaataaat
     8161 gtatgaatta tcttgttatt atggagaaaa gctgtttcgt ttatcttttt tgcatgaaaa
     8221 agtaaatgta catactcagt agctaggatg gtaacttatt acaatttatt gcgataaaaa
     8281 taaaagttgt tatttttatt gggaaaatac ccgatgtaga aaataatgcc ttccaatata
     8341 cctataaaat aagtccagta ttccaaagga ttatataaat aagtgacgta tctatctaaa
     8401 ggtgtaataa aaatgataag tgataataaa aaaataccat ttataactaa acataaacta
     8461 atggatttgg gcggtttcag gctcatcaga aaaatataga ttctggtgaa caacaaccat
     8521 aaaaaagtga gaatcattgc atgagagctt ctttccataa attttccttt aagagtagtg
     8581 cttaaaagta cagaaaaaat aataaatgca aggataggag caattaaagt aaatttattt
     8641 aataataaac aaaatttttc agctttttta ttcttggaat aacgaggtat tgttttcgat
     8701 aaaaaaagat ggtgcagaca aattatgata attaagcata ctatcagata actcaaaatg
     8761 aagccttctt tcaaaaaatt attcaaaacc atatattgta ttttttattt gataataaca
     8821 atgggattat taagaagtgg cactaaataa aagactagtg tgtatttagc cgatttaatt
     8881 ataaacaagt aaatggataa agtatatgta atgataaaag aaaaatggat tttctaattt
     8941 ttaagctaaa tgttagatca cacatgtata tgctggatag tcttataaaa aatcatgtaa
     9001 tacagcttgg tacatgattt ttatttgtag ctgtttttta catagacaca aaaaagaaac
     9061 cacgcttgta gacgtggttt cttttttact atctgtttcc agaagctgag gtgctgctgg
     9121 atggtgtatg gtcatttgca gcatccgctc cgctagttgc tggctcactg gaagaagcag
     9181 gaggagcaga tgaagacggt gtcgagcttt cttcggatga agaagaagga accgttgttg
     9241 aagacgctgg ctcgcttgtt tctgctgtcg tgctttcttt tgaggactca gcagttgatt
     9301 cgctttgact agtagagcta gagctttgag tcgttgtctc tgttgttgag cttccaggag
     9361 tctggacata gctggaagat ggaatagttg ttgatggtgt aattgcattc gatctagctg
     9421 tatattgatc tttgtagtac agttcaccgc ccacacgaat taatccgctt ggcatctccc
     9481 agtctgtatt tgtcacgtta gctgaaacat actgcataag ctcgcgataa acatcagagg
     9541 ctacgtgtga cgattctgaa gtgacaggtg tcattttttt attgtaacct gtccacacgg
     9601 aaatcgaata atttggcgta tagcccgcaa ataggatatc tggatacacc ccagaagaaa
     9661 taccaagttt tgcatactcg tcatctgtgt aattggatgt tccggtctta ccggcttgat
     9721 ataacccagc gatttgcgca ttcgtaccag ttccttcagt gatcgtatct tttaaaatgt
     9781 ctgtgatcat ataagctgtt tctggagaca tggctgtttt tccatctggc tcataagttt
     9841 cttctgttcc gtcttggaag acgattttat taacatattg aggtttgtaa taagtccctc
     9901 cattggcgaa agccgcatag gctgctgcca tttttaagga agaagctccg tattttgtcc
     9961 catcttgttc ttccgtatta ctagagatcg cattcgattg atggatcgta ctgtattcga
    10021 tcccaagatt ttttaagaaa gaagccactt tatctgaacc gacttcattg aataatttga
    10081 ctgctggaac attacgggat aagtaaagtg cttggcgtaa agtaatggtg cccatatatt
    10141 gattatccca gtttcctaca ggagtcgaag tcccttcata gttatacggt gcgtcagtaa
    10201 tagtttttcc agtagaatat ttcaaatatt caaaagctgg tccgtaatct gtgacaggtt
    10261 tcatcgtaga accaaaatca cgggaagtat tcactgctaa gttgttgcct aaagttacgt
    10321 cttctgcgat gtgacgtccg ccaatctggg cttttacttt cccggtattg gtgtcgataa
    10381 gggtggaggc aacttgcatc tcgtcgtctg ggtaagagac atactgatcc gtgttgacga
    10441 tatcatatag tttcttttgg gcgtccagat ccaaattcgt ataaatctct aaaccgtctg
    10501 tgtagacatt cttatctgtt ttttcttgta cttcattgat gacttctttg acataattat
    10561 cgacgatctt agtattgtca tcggattgag ttagttcttg cagaccatcc gttaccggta
    10621 cattaacagc ttgatcatat tctgtttgag agattttttc gttttgaagc atcgtataca
    10681 aaacagtatc tcgtcttttc ttcgcctgat ctggataaac ataaggatca taggcggaag
    10741 gggcttgagg catcccagca agcaatgctg tttgcggtaa agaaagttct gataatttct
    10801 taccaaagta catctcagaa gctgtctcca tcccgtacaa gccgtttgac atatacactt
    10861 tatttacgta gtaggtcaag atctcttgtt tcgatttttt ttgttccaat cttacagcca
    10921 tccacgcttc ttgggccttg cgtttcagag tctgatcctc ggcactggtg gagaagaagg
    10981 aaagtttgat caattgctgt gtcaaagtac ttccgccttg cagtccgcca gaggtaaagt
    11041 tagagagggc agaaccgatg atccgaatag ggtcaacccc gatgtgttta taaaaacggc
    11101 gatcttctac cgacacgatc gcatcttcta atgttttcgg taattcattc gcagaaattt
    11161 tttcacgctt ttctgcgcct aaatcttcga atagttcgcc atcttgtgta tatagtttgg
    11221 aagatacggt agcgtctaat tttttatccg tcaattcggg cgcatctttt gcgtaatacc
    11281 agaaaagtcc tacacctgct aaaaagagga tacagaaaaa tgagagtatt cctaaaagga
    11341 tcttgataaa taatcctttt ttatgtgttc cagaggactt tccagaacct ttgccagaat
    11401 tcttcttcac actcttttta ggagtaggct gtttctgtcg tctagagctt ctagtttgtt
    11461 catttgccat tcttgctttc tccttcaata gataatgttg ataaatagaa gtcaaccgcg
    11521 tccaaataag gaatccgcgg agaaatacca gttttgatct ctattcctag ttcttcgatc
    11581 aaagataaag gtagtgattt gcgtcctttt tccttttggg cgtcccagta agcaatcaaa
    11641 tgaacactat tcaaataaaa acagcgcttc aaagacgaaa accataacaa gacaaagcag
    11701 accccatttt gttcgaggca tgctttcata tgttcgattt gatgggcatg gaagttttta
    11761 aagggaaaag atgttttgtt cttggtttct tttgcttcaa agtccaagta aaagcctcga
    11821 tagacgccat tatagtctgt agttgaggct tgggtaaagt aggcttcctt gatcacagca
    11881 gcacttctct taggatagtc tactttcacg atttggatag gggtaggctt tttatggata
    11941 accgccaacc cgcgggaaag ataatattta ttactttcat tgattgcttc ttcaaagcgc
    12001 atcccccgat tgccgaattc agtgatctga tttctttttt cttgaatttt ttcgacggga
    12061 gaggcattct tataataagg ttggccattt ggatagcgca ataccatctt taatcacact
    12121 cctaggccac attatagcaa aaaagcggat agaaagaaaa ggaatctgag atggataagt
    12181 caaaagtaat ctatgtgtct gggtatcgaa gtttcgaact cggtgttttt aaagaaaatg
    12241 atcctaaaat ccaagttgtt aaaaatgttt taaaaaaaga actccatcag ttagcagaag
    12301 aagggctgga atgggtactt gtcagtggga atttgggcgt cgaactatgg gctgtgcaaa
    12361 tagtagcgga attgaaagaa gaatatccgg aactatctct tggtattttg tatccttttt
    12421 ctgattttgg taataactgg aatgaaggga accaagaaaa aaaacaatta gcagaacaat
    12481 tagcagatta tgtcgaagct gttagccatc aaccctacca atcacctagt cagctaaaga
    12541 atcacactcg ttttatactt gagcatacag tgggctgtct gctgatctat gatgaagaat
    12601 tccctggaaa aacgaagtat ttttttgaag atgcacagaa ttaccaagca gatcatccct
    12661 acgaaattcg gctgatcagt atggacgatc ttcaaaattt tagtgaataa tcgatattgt
    12721 gtttgattta tcaacttttt tcctatacaa tgaaaaaggt agaaatgtta ttacgtaaat
    12781 gaaaactaga ggtgtaaata tggcgaattt ggtttatagt cctaaagaca ttttacaaca
    12841 agaattcaaa acaaaaatgc gcggctatga tcccgttgaa gttgatgaat tcttagacaa
    12901 tatcatcaaa gattatgaaa cttacagcaa agaactcctt gctttacaag aagaaaacga
    12961 tcgattgagt gcaaaagtag ctcaattatc taaaactcaa ggagcagctc aaacacgcgt
    13021 gcaacaaaca gaggcaccaa agagcgcagc agtgaccaat tttgatatct taaaacgctt
    13081 gtcaaatctt gaacgtgaag tatttggtaa aaagcttgat caacaagcaa gtgcagtcaa
    13141 accagcacaa cctaatccaa ataactacac aaatgcagac acaagcttag atgataatga
    13201 aaaaacacgt caattttaat ttttgattaa tgaatttgcg gttttcgggt aatcgcggtc
    13261 tgacttgatc aggctgagga aagtccatgc tcgcacaggc tgtgatgcct gtagtgttcg
    13321 tgcttagcga aaccataagc taaggtactc tttggagtaa cggcaggaaa acaagctaag
    13381 gcttcggcta tgcttaagta tccttgaaag tgccacagtg acgaagcggc tggggaaacc
    13441 catccggtgg aacgcggtaa acccctcgag cgagcaaccc aaacaatggt aggggcgctt
    13501 tttgtctgga agaagaacag gacaaaaagg cagtataact gcagatagat ggttaccgct
    13561 ttgttttttc tccctggaaa agcaaagtac agaacatggc ttatagaaaa ccgcaaacat
    13621 cagggaatta ggtcgtgaca aaagtaatgc gttataagaa acaagaaaaa catttgaaag
    13681 ctatttttca atacttcaga tgaatcgttc ttattacaaa acaaactttt gtatcaccgg
    13741 ttattcggaa tttgagtaaa agtgtggggt aggactgatg tcccacctcc tttttttata
    13801 aataaggaga ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag
    13861 cgggctagaa gcactggttg gtaaagaatt aagagatctt ggaatcgaat gccaagttga
    13921 gaacggacgt gcacgttttg aaggaacaat ggaaacgatc gctacagcta atttatggtt
    13981 acgtacagca gatcgagtaa aaatcgtggt tggggaattc gatgcgtata cgtttgatga
    14041 attgtttgaa aacgtaaaag cattgccttg ggaagatttt cttccactag atgcagcttt
    14101 tcctgtagct ggaaaatcga tcaagtcgaa actatatagc acacctgatt gccaagcgat
    14161 cacaaaaaaa gcaatcgttg aacgtctccg tacctattat caccgtccag cttctgtacc
    14221 actgacggaa actggcgcac attttcagtt ggaagtagct ttgctgaaag accatgtgat
    14281 ggtaactctg gatacgacag gaccaagttt attcaaacgt ggttatcgct tagaaaaagg
    14341 tggagcacca ttgaaagaaa acatggcagc agcacttatt atgttgacaa attggcgaaa
    14401 agaccgtcca ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt
    14461 aataggccat aatatcgcac caggattcaa tcgtgagttt gcttgcgaat cttgggattg
    14521 gttcagtcaa gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag cagattatga
    14581 tattgaatta gatattactg gttcagatat caatggacgg atgattgaaa ttgccaaagc
    14641 caatgcagaa gaaatcggat taggctcttc gattaccttt aagcaacaag cagtcaaaga
    14701 cttcaaaacg gataaagaat acggggtcat cgtggctaat ccgccttacg gagaacgtct
    14761 aggagaagaa gaaactgttc ggaaattata taaagaaatg ggcgatgtat tccgtccatt
    14821 aaaaacatgg agtaaatata tcttgactag cgacttagcg tttgaggaat tttacggaca
    14881 aaaagcaacc aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc tattccagta
    14941 ttggggaact cgtccgccta gaaaaaaaga gaaagacgaa taatcttact aattgattca
    15001 ttcaattgga tgtttggagg aatagggaat gaaagaaaaa gagtttttac aagaagtaaa
    15061 agaaatcaat cttctacaac aagctttagg aatcctcgat tgggatacac aaacaggtat
    15121 gcctgaaaaa gctagtgcat accgaagtga agtagacagc tatctttaca gcatttattt
    15181 ttcaaaaaaa atcggtccaa aaatcaaaga agccattcgc tatttttctg aacatccgga
    15241 agaattgtcg gaagttggaa ctgccgtttt tgccaaagta aaagaagagt atgatttgga
    15301 acatcgcgta ccggaagatc ttatgagaaa atatacagct gccgtttctt cagcacacgg
    15361 acaatggcaa aaagctagag aaacacaaca attttctgat tttcaacacg cattgaatca
    15421 aaatattgaa ttaacaaaac agttgatacc ttactggaga aaagatgaaa agacgaatta
    15481 tgatgttttg ctgaatcaat acgaacccgg gatgactgtc gaaatccttg ataaggtatt
    15541 tgatcagctt aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa aagggactga
    15601 acctgaaaca gactttttaa atcggagaat gacgaaaaaa caacagaaga gatttgtagt
    15661 gaaagtaatc gaacaacttg gttatgattt tagtcgtggt cgtttagatg acactgtcca
    15721 cccatttatg actggaatta atccgaatga tgcacgaatc acgacacgat ggaacgaaac
    15781 agatttcaaa atggctgttt ttggagtgat ccacgaggct ggtcatggta tgtatgaaca
    15841 agacatcgat gataaatatg cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca
    15901 tgaatcacaa tcgttgttta acgagatcat tattggaagt agtcgcagct tctggaaaaa
    15961 gcaatatcct ttctttcaag aatgtgcaga aggaacattc gatgatattc cttttgacaa
    16021 attttatcgt ttattgaacc atacaaaatc aagtttggtt cgtatcgagg cagacagttt
    16081 gacttaccct ctccatatca tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg
    16141 ggtagatgtt gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat
    16201 acgaccaaca aatgatgtgg aaggcatcct acaagacgtt cattggtcag gtgcaagctt
    16261 tggttatttt ccctcttatg cattagggta catgtatgcg gcacaattac ttcattcttt
    16321 gcagcaagaa atagatgtgg aaaatgttct agcgtcagac gattattctc caatcaaaaa
    16381 ctggatgtca gagcatatcc atcaatatgg tgcatctaga aaaccaaatc aattaatcat
    16441 ggatgcaaca catgaaccgc ttaatccgca atacctgatc gactatatga aagacctgta
    16501 ttttaatgta tatcaggtaa aataaaatac aatttacaaa gatagctgaa attcatctaa
    16561 tctatagatg ttttcagcta tttacttaag aaagaagtgg aacgaatgga taaattacaa
    16621 gtgatcgctg attatagtat ggaccaatta gcgcatgatc aaacaggaca tgggagcgat
    16681 cataccaaaa gagttgtcaa gttagctgaa aaaattttgg agacggaacc gcaggcagac
    16741 cgctttgtga cgcttgcagc agcttaccta catgatacga ttgatgataa agtcgttaaa
    16801 aatgagaacg aggcaaagca acagcttcgt gtattccttc gctctttgcc aatagtagag
    16861 gaacagatct cgatgatttt ttccattatc gaaaatatgt ccttttctaa aaatctctct
    16921 gaagcggccg aactatctct ggaaggtaag atcgttcaag acgctgatcg tatcgaagca
    16981 ttaggagcaa tcggcatttt gcgtactgct tatttcggcg gtggacatgg gcacccgatt
    17041 tttgatcctg aactgtatcc gcaaacattc aaggacaaag aaaattaccg aaaaggcacg
    17101 acagtgatca atcattttta tgaaaaactg ttcttgctgt ctgataaaat gaatactgat
    17161 tacggatatg aagaagccaa acgaagagaa accttcatgc gtgaattctt agaagaattt
    17221 tttctggaat ggtctattga cgatgaaaat tttactccag acaattggat caaaaataac
    17281 aaaaaatgat ggaggttgtg acagaagtgg tcagctccaa gatatttcgg atcattttcc
    17341 gtaaaagtta cttctggaac actgtttacc aagaggtcgt gagcctgccg ttcagctccg
    17401 agtcacaagg aacaatttca aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg
    17461 attcggaatt ggcaggagaa caccgtttat tcggaagaat aggactgtga caagactttt
    17521 gtcacagtcc ttttttcgaa ttcaacaagt aaaactttta agaaatattc agagagtaaa
    17581 attaaaaatt gctgataaac gaagatggaa attttatgta tttttattgg tctatgcaat
    17641 aaataaatta aatattcaga atacaattga ttttaacgaa agggcttcta tcaatggttg
    17701 tgttgtattt atctgcccag gtagagtaaa atagatcatt atctaaaagg taattgttca
    17761 tactatataa gtcatttgaa agtaaaaaga tactgtaaat aaaaaatgtg gttaaataat
    17821 tctcataatc agtgtaagcg ttatcaaaaa tatagcatac ttgctttgta gatgaatgac
    17881 acgatatatg tttggtatgt tctgcaagac agatgaatat ggaggtggag acaatgtctt
    17941 ccaaagaact aacaatgtat ttagagaaag aattggatca actgaaagaa aaggggttat
    18001 acaatacgat cgacatacta gaaagtgaaa atggtgcttg tatcatcgta gacggaaaaa
    18061 agatgatcaa tctagcatcc aacaattatc ttggatttgc caatcgcgaa gaattgaaaa
    18121 aagcatgtat tgaagcaacc gaaacttatg gtgtaggagc aggagcagtc cgaacgatca
    18181 atggttcgct gaaaatccat cagcaattag aagaaaaaat cgcagaattc aaagggacag
    18241 aagcagcaat cgcctttcaa tctggattca actgcaatat gggcgctatc tctgcagtaa
    18301 tgacaaaaga agatgctatc ttatcggatg aattaaacca tgcatctatt attgacggtt
    18361 gccgcttatc cggagcaaaa atcatccgaa ttaaacatca agatatgaaa gaccttgaga
    18421 aaaaagcaaa ggaagcgata gaaagtaaga aatataaaaa aatcatgtat atcacagatg
    18481 gtgttttctc catggatgga gacattgcac gattgccgga aattattccg attgttgaaa
    18541 aatatggact gatcacctac gtcgatgatg cgcacggctc aggcgtaact ggaaaaggtg
    18601 caggaaccgt caagcatttt ggattaagcg acaaaatcga tatgcaaatg ggcacgttat
    18661 cgaaagctat aggtgttgtt gggggttatg tagctggatc gaaaacacta atcgactggt
    18721 tgaaagctag aagtcggcca ttcctatttt ctacttcgct aactccaggt gcagctgcag
    18781 ctgcattggc ttcgattact ttgatgcaag aacatccgga acttgtcgaa aaagtttggg
    18841 aaaatgctaa ttattttaaa gaagaattga aaaaagtagg ctataacatc ggtatgtctg
    18901 aaacacctat tactccagtc attttgggag acgaaaaagt gactcagact ttctctaaga
    18961 agctgatcga acatgggatt tatgctaaac caatcgttta tccaactgtg cctcttggga
    19021 caggcaggat cagaaatatg ccgactgcgg aacatacgag ggaaatgctt gatgaagcag
    19081 tagcagttta tcaaaaaata ggaaaagaaa tggaaattat ttaactaaat ttagtttttt
    19141 cgtctattta aaattagaga acatgttcac gcatatattg ttaatcatct gaagatagta
    19201 gtaaaaaaga tcagggagga tcatcatgaa aaaagtattg gtgacaggat gtttaggaca
    19261 aattggttca gagttgatca cacggctacg tcaagaacta ggcagtgaaa atgtcattgc
    19321 gacagatata cgcatgcctg aagtcattga gaaaaacgat atctttgaga tcctagatgt
    19381 aatggattac gaaaaaatgc gcagcatagc agaacaatat caagtggaca cattgattca
    19441 tttggctgct cttttgtcag ctgttgcaga agcaaaacca caattagcct ggaatttgaa
    19501 cgtgactggg ctggtcaatg cattggaagt cgctagagaa ttccagctga aattttttac
    19561 gccaagttca atcggcgcgt ttggtccaaa tactccaaaa gatcataccc cacaagatac
    19621 tcttcagcgt cctacaacca tgtatggggt aacgaaggta gctggagaac tactatgtga
    19681 ttattattat gtgaaatatg gcgtagatac tcgtggtgtc cgtttccctg gattgatttc
    19741 ttatgcaacc ttgccaggag gcggtacgac agactacgca gtcgatattt attattcagc
    19801 aatcaaagaa ggaaagtaca ctagtttcat tgatcaaggt acatcaatgg atatgatgta
    19861 tatgccagat gcgatcgatg ccattataaa actcatgaat gcagatcctg atcgattggt
    19921 tcatcgaaat gccttcaata tatcggcgat gtcttttgac ccggaagaga tcaaagcatc
    19981 tattcaaaaa atcataccag attttgaaat ggattatgat gtggatcctg tacgtcagtc
    20041 aatagcagaa tcttggccaa attcactaga tgtttcttgt gcgcaagagg aatggggctt
    20101 ttcgccgaaa tacgacttag atgtgatgac aaaggatatg ttggaaaaac tgaaagaaaa
    20161 attggtagag acggtataaa acaagaacca catccaaaaa gttttttata cttttgggat
    20221 tggctatggt atgaaagggg acaagggatg gaagaatatc aagccacgcc agtaaaagaa
    20281 gtaaaaatag aaaatgaatt aaaacgtacg atgggatttt ttactgcttt atcgactgtt
    20341 atgggcacag tgatcggtgc aggggtattt tttaaagccg caagcgtagc agaagttaca
    20401 ggatccgcca gtttgcatat gttttcttgg tttctcggtg gtatgatttc tgtctgtgca
    20461 ggactaacag gagctgaact ggcagctgct attccagaaa caggagggat gattaaatat
    20521 atcgaacgga tttacggaaa tacagcagct tttttattgg gctgggcgca agtcgtgatt
    20581 tattttccag caaatgtagc agccttgtcg atcattttcg gtacacagtt cgtcaatcta
    20641 tttggtcttt cccagtcaat gattgttccc gtagctgtta cagctgctgt atcgatcctg
    20701 ttgatcaatt ttttaggttc taaagctgga ggggcttttc agtcgattac gcttgtctgc
    20761 aagcttattc ctttgtttgt gatcgtcatt ttcggtttgt tccgacaaga aggagtagac
    20821 ttccaactgt ttccgatcca agcaggagaa aatctttcat ttttctcagc cttgggagcg
    20881 ggcttgctag caacgatgtt cgcatacgac ggttggatac acgtaggaaa tatttcggga
    20941 gaattgaaaa agccggcgaa agatttgcca aaagcaattt ctttaggaat catcggaatc
    21001 atgatcgttt atttattagt aaatgcggtg ttcttaagaa ccgcatctat cgacggagtc
    21061 gcaggaaaca gcaatgcggc aagtgacgtt gcaaaaatga tttttggcgg cttcggtgga
    21121 agactggtca cagttggtat cttgatttct gtttatggaa ccatcaatgg ctataccctt
    21181 actggaatgc gtctccctta tgttatggca aaagaaaaca acctgccttt tagcaagctc
    21241 tttgctaaac ttcatgataa aactaaagtt cctgtagccg caggtatctt agagctagtc
    21301 atcgctattg gcatgatgat gatcggtggt tttgatacac taacggatat gctgatcttt
    21361 gtcatttgga ttttttatac gatggtcttt gttggtgtca ttcttcttcg taaaaaagaa
    21421 cctgatttgt ttcgaccata caaagttcca atgtatccgt ttattccact agtagcaatc
    21481 attggaggga cttttatcgt atcaagcacg cttatcactc agaccttttt agcttcgatg
    21541 gggatagtat tgacacttgc tggtatcccg atttatctct atttaaaacg tagatagcta
    21601 aaacattcga attttattag aactgcactt ctctgccaaa caaaatattt tatttaaatg
    21661 aggagtacgt ggaagaaatg tcaaaagtac tcgttaattt tgatcacgta aatgtcgttt
    21721 tgtctacgat gaaatagcaa agtaaaagac agaaatcggg ctcaaccgat ttctgtcttt
    21781 tactttgatt tgcgtaactg aactattcgg cgtacgccgc gataaatatt taataaaacc
    21841 aagcaaccag aaatgaaaat gggaagccaa atcagataga cagggtagcc aagcacttta
    21901 gacaaagcat gtggagtcat tttatccatt tcaagaagtg cttgctgcca agtctgaaga
    21961 ttgaagacta gcatgaaacc cgaaataatc aggagaataa atcctaaata atgaattgta
    22021 gttctttttc ttacataagc gatcgtagca taaaggaaca gtaaaaaaat aggtacgcca
    22081 aaaacaaagt acatattcag catctcgaca ccccctttgt gtttatctta ctttactttg
    22141 aaagaaatga gaaataatag cctcgttact tgcttgaaac agcaaaggac tgctaagata
    22201 agagagatac aaagaatcaa ggaggagtca tatggcaagt tatatcgaac cgattcgttt
    22261 tgcgctgatt gtttttccat ttttagcctt agcgatctca tctgtttttt ttatttatga
    22321 atatcgaaag tatggaacat ttctagtaac aagggcagtt attttgtatt ctttcgtttt
    22381 ttatttattg tgtgcgtatt ttttggtgat cttaccactt ccggcaaagg agacggttgc
    22441 ccaaatgaca gggccaaaga tggagctgca tttatttgct tcatggcaac atttcagaga
    22501 caacacggta ttggtcttgt ctgaacctag tacctacctg ccggcaatga agcagagtgt
    22561 atttttggaa ccagctttta atgttttatt actcatacca tttggcattt atttaagata
    22621 ttatttccgg ctttctttta tgaaaacgat gattgctagt ttctgccttt ctctattttt
    22681 tgaattgacg cagctaagtg gactttattt tatctaccca agaccatacc gcttatttga
    22741 tgtgaatgat ttagtgacaa atacattggg tggaatcatt ggttttgtcg ttacgccgtt
    22801 atttacattt atgcttccta caagaagacg aatggatgaa ctttcttatg aaaaaggaaa
    22861 gactgtctca ttgactcgac gcttggtcgc ttatttgatc gactggacat ttttatctct
    22921 tattaatttg gtagcggcga ttttctttaa aattgcgttc gattcagggg cagttacttc
    22981 tcatatctgg tggcttttat tagaagtagg ggtatacttt atgattgtac cgtttataac
    23041 caatggatgg acacttggca aaaaaattgt gcgtatccgt ttggtggaag aagaccgcga
    23101 acgaatttct ttaaaagcat tgatgatccg ctacgggtat ctgtactttc ttttttatgg
    23161 tttaagctgg ttggggcagt tgcttcagca aatcatgaat tcgacaacga atacggtgtt
    23221 gcagatgagt gcagtattca tttctctcgg tgtcttgtta ctacaaggta tttttatttt
    23281 aaatatcttg ctatcatttt tgagaaaaaa acggattctg ttttatgaaa aagctagtca
    23341 cacatatact gttagcacga ttggccaaac aaaggatgat gattctcgaa attagctgag
    23401 caaaaacgct ttgtctgtgt tataatgcca aagtcgtgat aatttaaaga gaagaggtgc
    23461 acgaatgaat aattttgata tattccgctt caaaccgttt atcaatgaag cgttaaccgc
    23521 aaagggcttc aaacagccca cagaggtcca ggaacgtttg attcctttaa ttgcaaaagg
    23581 aaaaaatatc attgggcaat ctcaaactgg tacaggaaaa acacatacgt ttttgttgcc
    23641 tttgtttgaa aaaatcaatg cagaaaaaga ggaagtacaa gttgttatta cagctccaag
    23701 tagggaacta gctgcacaga tctatgaagc agctacacaa atcgctaaat tttctacacc
    23761 ggagatccgt gtctcgaatt ttgtcggcgg tacagacaaa cagcgtcaat taaatcgtct
    23821 gcatcaccaa cagccacagg ttgtagtagg cacgcctggt agaatattag acatgatgaa
    23881 tgaacaagcg ctgaagattc acaccgcatt tgcatttgtt gtcgatgaag cggatatgac
    23941 attggatatg ggttttttaa atgaagtaga ccaaattgca agtcgattac cagaaaagct
    24001 gcagttttta gttttttctg ccactattcc agaaaaactt cgtccattct tacgaaaata
    24061 catggaaaat ccaatcattg aagaaatcaa accaaaagca gttatttctg aagacattga
    24121 aaattggctg atttcaacaa aaggtaaaga tgagaatcaa atgatttatc aactgctgac
    24181 aattggtcat ccttatttag caatcgtatt tgccaataca aaacagcatg tggatgaaat
    24241 tgccgattac ttgaaaaacc aaggcttgaa agtagcaaag attcatggag acatcacccc
    24301 aagagagaga aaacgagtga tgcgacaagt acagaatctg gagtatcaat atgttgtagc
    24361 gactgattta gctgctagag ggatcgatat cgaaggtgtc tctcatgtta ttaatgcaga
    24421 aattccagaa gatctagact tctttatcca tcgtgtcgga cgtactggac gtaatggctt
    24481 aaaaggaacg gccattacgt tatattcgcc aaatgatgag caagccattg atgcgataga
    24541 aaaattgggc gtctcatttg aaccaaaaga aatcaaaaac ggggaaatcg tcaaaacata
    24601 cgatcgtaat agacgtgcca aacgagaaaa atcaaaagat gtattagatc ccacgctgat
    24661 tggtcttgtg aagaagaaaa agaaaaagat caagcctggt tataagaaaa aaatcgattg
    24721 ggcaattgca gaaaaaaata aaaaagaacg caaaatcgaa cgcagacagc aaagccgtac
    24781 tgctcgtaaa aataagaaaa attcacataa ataaaaaaca agaggttgtg acaaaaggcg
    24841 tgtcacaatc tcttgttttt aagctagact ttttttcatt ccgatacaaa gtacttcatc
    24901 ttcaataaaa ggttctgtgc aagtatgata cccttgtttt tcataaaaat ctacagccgt
    24961 catttcagct actacataac tagtctgaca accttctgaa accgcccgtt tttcagcagc
    25021 ttcaagcaac tgaccgccta acccttttcc tcggtattct tttgctacgc aaaatcgttc
    25081 cggcttgaag caagtaggtg ttttcttttc ataacgcaaa gtagctactg gtcttttttg
    25141 atcaagaaat agataatagg tttgttgcgg gatatctttt ccgtcgaatt ctagttctgc
    25201 aggaatccct tgttctaata caaaaacttg atatctcaaa aagcagctag ctgcgcgtat
    25261 ccattcatcc gctccaacta tttttttcat catattcctc cttgtcatga atagcagaga
    25321 tgtattgatt cctttattct tatcgtacca aaaatcgtgg agtatgacca aaaggaaatg
    25381 aaaataaaaa ttgagatatt gacagtgatc aatagaatgc gtatactaat taagtacgaa
    25441 aaggatatga tttgtttttt aagactcaca gaaaattctt tcttggctga aaaaagaata
    25501 gtcactaatc aaattgctcc ctttttctat tttggtagaa ataccgacgc agctcagcgt
    25561 tatcgagttt aagaggtaat gattacacat cattgcaatc aaggtggtac cgcgagtttt
    25621 cgtccttggc tgtgatggtg tttttttgta tttaatttat agaaagcagg taatgacatg
    25681 aaacaattaa gtagtgcaca agttcgacaa atgtttcttg atttcttcca atctaaagga
    25741 catacgattg aacctagtgc gtctttgatc cccgtgaacg atccaacctt attgtggatc
    25801 aactctggtg ttgcaacatt gaaaaaatat tttgatggtt cagttgtgcc agaaaatcca
    25861 agaatcacaa atgcccaaaa atctattcga acaaatgata tcgaaaatgt cggtaaaaca
    25921 gcacggcacc atacgatgtt tgagatgctg ggtaattttt cgattggtga ctatttcaaa
    25981 aatgaagcca tccactgggc atgggaattt ttgactgatc caaaatggat ggcattcgat
    26041 ccagaaaaac tttatgtaac agtttatcca aaagatacag aagcaaaacg catctggcat
    26101 gaagaagttg gcctgccact agatcacatt attgatgtag aagacaactt ctgggatatc
    26161 ggtgcaggcc catgtggtcc ggatacggaa attttctatg accgtggaga agcttataac
    26221 gatttagcag aagacgatcc tgaaaactat ccaggaggag aaaatgaacg ttatttagaa
    26281 atctggaact tagtattttc agaattcaac cataagcctg ataacacatt tgaaccactt
    26341 cctcataaaa acattgatac ggggatggga ttggaacgca tggtctcaat cgttcaagat
    26401 gcgtcaacga actttgagac agacttgttt atgcctatta tccacgccgt agaaaagcta
    26461 agcaaacaag taaaatatgg tgaaaaccca gtaacagata tttcttacaa agtaatcgca
    26521 gatcatattc gcgccctttc atttgctatt ggcgacggtg cattgccatc gaatgaaggg
    26581 agagggtatg tcttacgccg tttgcttcgt cgtgctgtca tgcatgggaa aaaattaggt
    26641 attgaacaag cattcttata tcgtttagtc cctgttgttg gggaaatcat ggtaagttat
    26701 tatcctgaag tacttcaaca aaaagaattt attgaaaaag ttatccgtac agaagaagaa
    26761 cgtttcaatg aaacaatcaa tgaaggtttg gaaatcttaa atcaagtgat ccaagaagta
    26821 aaaaacgaaa acgggacgac tctagcagga aaagacatct tcaaactata cgacacatat
    26881 ggcttcccgg tagaattaac ggaagaggtg gcagaagatg aaggactgaa agttgatcat
    26941 gctggttttg aaaaagaaat ggatgcacaa cgcgaacgtg ctcgctctgc ccgcagtact
    27001 gaaaaatcaa tgggggtaca atctgcttta ttgacagata tcaaagtaga aagtaaattc
    27061 attggataca cagaacaaga atcagatagt caattattgg tcatcgttaa aggtgagtct
    27121 cttttgaatg aagtcgcaga aggtgaagct caaatgatct ttgatcaaac acctttctat
    27181 gcagaaatgg gtgggcaagt agcggatcaa ggatggatca tgaatgaagt aggcgaaatc
    27241 gtagcagttg ttacagatgt tcaaaaagcg cctaatggtc aatttatgca caccgtagaa
    27301 gtaaaagcac caatgagtga aggacaaacc tatcatctat tgatcgatca atcgatgcgc
    27361 aatcggatca tcaaaaacca tacagcaacc catctactgc acaaggcatt gaaagaagta
    27421 cttggcgagc atgcaaatca agctggttct ttagtcgctc ctggacattt acgattcgac
    27481 tttacccact ttggtcaaat cacttcagat gaattagctg aaatggaaag aatcgtcaac
    27541 gaaaaaatct gggaagctat cccagttgaa acaattgaaa cagatatcga tacagccaaa
    27601 aacatgggtg caatggcttt gtttggtgaa aaatacggac atgacgttcg agtagtaaat
    27661 attggcggct ggtcaatcga actgtgcgga ggcacacatg tagcaaatac agaaaacatc
    27721 ggaatcttca aaatcgtttc tgaatcagga attggtgcag gcgtgcgacg aatcgaggct
    27781 gtaacaagta aagaagcgta cgaactactt gaagaagaag aaaaacaact aaaacaagta
    27841 gcaacaattg ttaaatcacc tcaattgaaa gaagttgttt caaaagcgga acaattgcaa
    27901 cagcaagtaa gagagctaca aaaggaaaat gaacaattag ctagcaagtt agcgaatcaa
    27961 caagctggtg atgtattcaa agatccagaa acgatcaatg gaaaaacaat cattgcagca
    28021 caagtaaaag tgaaagatat gaatcagctg cgtcaattag ctgaccaatg gaaacaaaaa
    28081 aatatttcag atattcttgt tttagcaaac gaacaagaag gaaaagtcag tctgctagcg
    28141 gcaatgacga aagaatcaaa tgaagctgga ctaaaagctg gcgacttgat taaagcaatc
    28201 gcaccgaaag taggcggtgg cggtggtgga cgtccagaca tggcacaagc tggcgggaaa
    28261 aatcctgctg gtattccaga tgcacttaaa gctgcgaaag aatgggttgc gcaacaatag
    28321 ggcgtttacg tttgacagaa aaacaaaagt tcaaacattg aacgaaacat gattatgggg
    28381 gcgaagacaa acaagtcttg ccctcatact tttttcaatt ggatggcgca tctaatcaaa
    28441 tggtcggatt cggatggtct ttagttgacg aaagtgacag aaatttttac gattagtata
    28501 aataaaaagg ggtgaaaaaa tgaatagtag aaataagacc tttcgactag tcttacgtgc
    28561 gatcttgtta gcaatcatca tcgtacaagc gatggttcct tggctggggt ttattccatt
    28621 aggattcatt agtttaacaa ttatccatat cacagtaatt attgctgcag tagtattagg
    28681 cccaaaagat ggcatggtga ttggcttgtt ttggggaatc gctacaattg ttcgtgccta
    28741 tgccatgcca acaacgccat ttgatacatt agtgttcacg aatccgatta tctcggtagt
    28801 tcccagagta cttgttggct tagtagcagg cttggttttc cactggattt atcaaaaaaa
    28861 taaatcaata acgatcagct cagcctttgc aggggtactg ggttcattgg tcaatacagt
    28921 cttagtgtta ggattcatgg gattatttta tacaggtgca acggcagatg cctatggtgt
    28981 agactctgct cttttattta aaaccttagc aggggttgca gcaatcaatg ggataccaga
    29041 agcaataggt gcgggaatca taacaccatt actagcaaaa gcgttatttg cagcaacacc
    29101 gttgaaacca gaataatgac catcttttaa tagtaatagg acgcacgata gatagaaaaa
    29161 ggaagagagg agaggaaaac aatcgtcctc tccatctttt tacctgagca acaagcaaac
    29221 tagaaggggt aaaaaacttg gctaagaaac aagaagaaaa aaagaaacaa ataccgcaac
    29281 agcggacaaa aaaaggagta ctagcgggta tcttaggatt ggttacgaat ctgatcttgt
    29341 ttgtctctaa gctaatgatc gggctttttt caggaagtgt ctccattatg gctgatgcag
    29401 tgaacagtct atcagatact gcttcatcca tattgacttt gatcggattt aaaattgctg
    29461 caaagccagc agatcaggaa catccttatg gacacgaacg cttcgaatat atcagtgggt
    29521 tatttgtttc gatcatcatt acttatgtag gatttcaatt tttagagtct tctgctgaaa
    29581 aaatcatcca tccagaaaat gtctctttaa cacccatcgt cttttttgtc ctaatcattt
    29641 ccatcttttt gaaatttgca caaggcaaaa tgtatcaggt tattgcgcaa tccattgatt
    29701 ctgaaacatt gagagctaca gcaacagata gttataatga tgtctttact acactggctg
    29761 tattgctttc agcagcaatc gaatgggtaa ctggatggcg tattgatgga tatataggtt
    29821 ttgctttagc attatatatc ctttatagtg gattgacgat gcttcgaaat tttgtatacg
    29881 aactattagg aagtcgccca acgccagcag agattgaaga aatggaagag catttaaatg
    29941 cttgcgatga tatacttggc tatcatgatt tattggtgca taactatgga cccaataaac
    30001 gctttgcatc agtccacatc gaagtagatg aatcaatgaa tttgaatgtg gctcataaga
    30061 ttattgatct gatagaaaaa gactttaaaa agtctttgga tgtggaactt gtctgtcact
    30121 tggatccaat gcctgttgat aacgaaaaat actataatat cttgcggcag ttaaagcaaa
    30181 ttcttcttga aacaggtaca gggcttaata ttcatgattt tagagtaatt catcaaggaa
    30241 aaacgctgca atttgatgta gtggtccctg agaaattcca gtttgttgat acagaactag
    30301 aaagaatctt acgtagtcaa atagaagaaa aaatcggaga ttaccaattg gaaattacgt
    30361 ttgatcacaa ttatctttta tattaaatga gaaactataa aaagactc
//
LOCUS       DACDIP010000027        29949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDIP010000027 DACDIP010000000
VERSION     DACDIP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.027"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..328)
                     /locus_tag="IV257_001428"
     CDS             complement(26..328)
                     /locus_tag="IV257_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8814564.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IV257_001429"
     CDS             complement(353..2446)
                     /locus_tag="IV257_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8814565.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IV257_001430"
     CDS             complement(2935..3699)
                     /locus_tag="IV257_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8814566.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IV257_001431"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IV257_001431"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8814567.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IV257_001432"
     CDS             complement(4704..5780)
                     /locus_tag="IV257_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8814568.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IV257_001433"
     CDS             complement(5804..6397)
                     /locus_tag="IV257_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8814569.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IV257_001434"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IV257_001434"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8814570.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IV257_001435"
     CDS             complement(8067..8366)
                     /locus_tag="IV257_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8814571.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IV257_001436"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IV257_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8814572.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IV257_001437"
     CDS             complement(10121..11032)
                     /locus_tag="IV257_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8814573.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IV257_001438"
     CDS             11152..12009
                     /locus_tag="IV257_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8814574.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IV257_001439"
     CDS             complement(12196..12558)
                     /locus_tag="IV257_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814575.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IV257_001440"
     CDS             12904..14343
                     /locus_tag="IV257_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8814576.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IV257_001441"
     CDS             complement(14350..16902)
                     /locus_tag="IV257_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8814577.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IV257_001442"
     CDS             complement(17016..18347)
                     /locus_tag="IV257_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8814578.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IV257_001443"
     CDS             complement(18313..19791)
                     /locus_tag="IV257_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8814579.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IV257_001444"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IV257_001444"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8814580.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IV257_001445"
     CDS             22401..23828
                     /locus_tag="IV257_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8814581.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IV257_001446"
     CDS             complement(23920..24285)
                     /locus_tag="IV257_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8814582.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IV257_001447"
     CDS             complement(24282..24647)
                     /locus_tag="IV257_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8814583.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IV257_001448"
     CDS             complement(24839..25087)
                     /locus_tag="IV257_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814584.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IV257_001449"
     CDS             25086..25214
                     /locus_tag="IV257_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8814585.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IV257_001450"
     CDS             25271..26965
                     /locus_tag="IV257_001450"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8814586.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27032..27238
                     /locus_tag="IV257_001451"
     CDS             27032..27238
                     /locus_tag="IV257_001451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814587.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="IV257_001452"
     CDS             27616..28749
                     /locus_tag="IV257_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8814588.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="IV257_001453"
     CDS             28780..29100
                     /locus_tag="IV257_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814589.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="IV257_001454"
     CDS             complement(29129..29593)
                     /locus_tag="IV257_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8814590.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29725..>29949)
                     /locus_tag="IV257_001455"
     CDS             complement(29725..>29949)
                     /locus_tag="IV257_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8814591.1"
                     /translation="LVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLA
                     AVNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         9034 a         6212 c         5169 g         9534 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattactta ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
    29701 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
    29761 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
    29821 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
    29881 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
    29941 actaactaa
//
LOCUS       DACDIP010000028        29857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDIP010000028 DACDIP010000000
VERSION     DACDIP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.028"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1154..1552)
                     /locus_tag="IV257_001456"
     CDS             complement(1154..1552)
                     /locus_tag="IV257_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814592.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1768..2823
                     /locus_tag="IV257_001457"
     CDS             1768..2823
                     /locus_tag="IV257_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP8814593.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2843..3196
                     /locus_tag="IV257_001458"
     CDS             2843..3196
                     /locus_tag="IV257_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814594.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3378..3674)
                     /locus_tag="IV257_001459"
     CDS             complement(3378..3674)
                     /locus_tag="IV257_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814595.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3678..4496)
                     /locus_tag="IV257_001460"
     CDS             complement(3678..4496)
                     /locus_tag="IV257_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8814596.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4722..4943
                     /locus_tag="IV257_001461"
     CDS             4722..4943
                     /locus_tag="IV257_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP8814597.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4945..5211
                     /locus_tag="IV257_001462"
     CDS             4945..5211
                     /locus_tag="IV257_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8814598.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5324..5863
                     /locus_tag="IV257_001463"
     CDS             5324..5863
                     /locus_tag="IV257_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814599.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
     gene            6072..6254
                     /locus_tag="IV257_001464"
     CDS             6072..6254
                     /locus_tag="IV257_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP8814600.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            6357..6641
                     /locus_tag="IV257_001465"
     CDS             6357..6641
                     /locus_tag="IV257_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814601.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6657..6893
                     /locus_tag="IV257_001466"
     CDS             6657..6893
                     /locus_tag="IV257_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814602.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6915..7169
                     /locus_tag="IV257_001467"
     CDS             6915..7169
                     /locus_tag="IV257_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814603.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7251..7436
                     /locus_tag="IV257_001468"
     CDS             7251..7436
                     /locus_tag="IV257_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8814604.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7503..7898
                     /locus_tag="IV257_001469"
     CDS             7503..7898
                     /locus_tag="IV257_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8814605.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7888..8265
                     /locus_tag="IV257_001470"
     CDS             7888..8265
                     /locus_tag="IV257_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814606.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8266..8661
                     /locus_tag="IV257_001471"
     CDS             8266..8661
                     /locus_tag="IV257_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814607.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8968..9366)
                     /locus_tag="IV257_001472"
     CDS             complement(8968..9366)
                     /locus_tag="IV257_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8814608.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9558..10325)
                     /locus_tag="IV257_001473"
     CDS             complement(9558..10325)
                     /locus_tag="IV257_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8814609.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(10360..11583)
                     /locus_tag="IV257_001474"
     CDS             complement(10360..11583)
                     /locus_tag="IV257_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8814610.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            12563..13366
                     /locus_tag="IV257_001475"
     CDS             12563..13366
                     /locus_tag="IV257_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8814611.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            13951..14406
                     /locus_tag="IV257_001476"
     CDS             13951..14406
                     /locus_tag="IV257_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814612.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            14525..15583
                     /locus_tag="IV257_001477"
     CDS             14525..15583
                     /locus_tag="IV257_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8814613.1"
                     /translation="MKKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15625..17574
                     /locus_tag="IV257_001478"
     CDS             15625..17574
                     /locus_tag="IV257_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8814614.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            17577..21218
                     /locus_tag="IV257_001479"
     CDS             17577..21218
                     /locus_tag="IV257_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814615.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            21618..21956
                     /locus_tag="IV257_001480"
     CDS             21618..21956
                     /locus_tag="IV257_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814616.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            22066..22287
                     /locus_tag="IV257_001481"
     CDS             22066..22287
                     /locus_tag="IV257_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814617.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            22307..24463
                     /locus_tag="IV257_001482"
     CDS             22307..24463
                     /locus_tag="IV257_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814618.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            24475..24708
                     /locus_tag="IV257_001483"
     CDS             24475..24708
                     /locus_tag="IV257_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8814619.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            24698..25564
                     /locus_tag="IV257_001484"
     CDS             24698..25564
                     /locus_tag="IV257_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814620.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            25579..27549
                     /locus_tag="IV257_001485"
     CDS             25579..27549
                     /locus_tag="IV257_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814621.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            27716..27943
                     /locus_tag="IV257_001486"
     CDS             27716..27943
                     /locus_tag="IV257_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814622.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            27940..28434
                     /locus_tag="IV257_001487"
     CDS             27940..28434
                     /locus_tag="IV257_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814623.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFVL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            28609..28944
                     /locus_tag="IV257_001488"
     CDS             28609..28944
                     /locus_tag="IV257_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814624.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(29201..>29857)
                     /locus_tag="IV257_001489"
     CDS             complement(29201..>29857)
                     /locus_tag="IV257_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814625.1"
                     /translation="KPKQQVLTEIEYYYRLRSLAVLGNNNEELQNHLNSLVDPILQQK
                     MRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDASSFRLADKDMEVLPWIYNKL
                     NQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKFQVNLRKHYSGLLYPNAVTM
                     DQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDYLRKEINSEKELLNYVHKIK
                     "
BASE COUNT        10687 a         4483 c         5371 g         9316 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac cttgcgtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgaacaagt ttttacctat tcccaaaatt aatttattcg ttattttttc attgattaag
      181 aataattatt tgatgcttaa attagctaaa aaaacgattt tttactgata taatcatgat
      241 attaaagctg aattaggcgg aaagataaaa tgttcgcaaa atcaaagtat caattatctt
      301 gtttttttat aaatttgtaa gtaaaaatct aaaaagtttt cttttaattg gtatatgtat
      361 taaaatcaca ttcaatcact cataaaagta tgcgctttca tttttttgga tgagattaat
      421 atcaataaat aacgatgtaa aaaccattaa ttatgctctt ttataaggat attaattaat
      481 ctaatttgaa aaaataattc aaaatttaaa tgttttaata ttacaaataa aatcatgtta
      541 tcgtaaaatg gtataaaaaa gctaaatatt ctatttttgt tattttatta gcagggaaat
      601 ttaaatagtt tacaagaatc cgtagaaata tattggtttt gccttcagat taatactgga
      661 tcatattaat ctgaatataa tatatgaact aataaatagc ttaacctcgc tattttgttt
      721 tttctgatca ctatctatac agcattagta aatagaattt taaaaataaa aaaattaaga
      781 caaaaaaagt atcaacataa acatatacat atacacgttt atgttgatac acttaatttg
      841 tcctttaaaa agcacttatt ttaatattat gttattttat aatcttatta caagctgatt
      901 gttagacgtt gtcctttcat tacatttatt aacggttatt ttatcgttta atttattaat
      961 tatttttgat ctaaaactta gcttaacaaa taaataatta accaaccgcc agataaaaat
     1021 gcgatcattg ctaatacata agcaaagata aaaatatgtt cgatcagctt aaaccctgat
     1081 gcgattaagc agaccaagta aaatagaaat tctcttacag ataatggtaa ttttctaact
     1141 agcatactca taattaacac cccaaagtaa tattgtattt tgaacatttt acatccaata
     1201 cagcatcaat caaccattgc caatcaaaaa tacaaggctt cattgaagta tgtttttcag
     1261 atgaaaagat ataatataat ttcttcggaa taatattttc agttgttggg atcaagctaa
     1321 atttaatacc atctaactca tagctaccag ctttctctcc attaaaaatt tccttagcac
     1381 tttcttctaa agaatcaaaa tatttttcga cagcaacctt tgaagctttt actattgaag
     1441 catttccctt ttcgataaaa tctgcttttg ttttaccgac aataagtaat tcttgatcag
     1501 taaaatcttc taaaataata gtagtgtaat tcttatttct tctcaatcgc atcaggatca
     1561 ctccttaata taatttatgt ttttataata tcataataag ataaatttgc aagtgtttat
     1621 ttacaccttt ttacttacac tttattgtaa ttgattatac ttttttgatt gcattttttt
     1681 cttataaaca gttgcaacta ttagtaaata ggtgtaaact attttcgtac cgaaatacca
     1741 taaacacaaa cagaaagagg tcataccatg aaagaaccat tattgttttc cgttgactta
     1801 gggaataaat cagtaaaaga aatgagtaca cacgtttctc cactctcgct tccctctcgt
     1861 attttgaaca aaaatatcat taattcagga cgtttttcta atataggttc ctcattaaaa
     1921 cgatcactaa acgtttctga atatagccta gtaaacacta ctgaaagcta ttattttggg
     1981 aaggatatac acgagctagg aaaagatgac tacgtgatag aatcactagg ttttgggatc
     2041 agcagataca agcaggaaac ctaccaagat atcctatctt tctctttagc taacctagca
     2101 cgtttagaag atcaatctga tgacgtttta accgttaatc tagttttagg tctgccttca
     2161 tccgactata ccgaaacaat cgtcaaatac gtgatgcaac aagccacacg acaacattca
     2221 gttgaagttg acggtaaagc ttataatata aacgtagata ccgtttgggt actgccacag
     2281 ccgattggaa cactatataa cctcctcctt gctgatgatg gtagtgtaca agatgaatca
     2341 ttgatcagtc agaagatcgg ttttgttgat attggtggtg gcacgaaact ttttgatgtt
     2401 gtccaaaatt ttgaaattga cgaaaacaac cgtgaacaaa aggataccgg tatttttact
     2461 ttatataatc gtatcgtaaa tatgcttgac cttccgatca gtcctaatgc tttccaactt
     2521 gaaaagatta tccgagaagg caaagaaaaa ggcgtatatc tatttaaacc aagcggaaat
     2581 gaaagttttg atatttcaga agtagttgaa agagaaataa aaacatacac caatatggtt
     2641 gttgcagaag ttttagctac attcaaaaac ttcacaacct tagataaagt tgtctttact
     2701 ggtggtgggg cttctattat cgacaaacaa cttgtaaaag ataaaattcc gcataccttg
     2761 tttgttgaag acagtgaatt ttctaatgta aaaggctttt acaagtttgg tttaactcaa
     2821 taaaacagcg aaggagatca acatgaataa gggaaaatca gagcgttatg tagttataat
     2881 caatagagaa aaggttagcg agttaccttt agcagagttt cttgaatctg aatatgaaac
     2941 actaggcgtt aaatactctc agacgatcaa aaaagctttg aatctatatc ggcgtgtaaa
     3001 gaacaatgaa gttacaatta ttgaagcagg caacgataga aacgatagca aagtagctga
     3061 agtaaagaat aaaccaactg atcataaccc tattcaacaa gcgcatcctt caacggttac
     3121 atccgatcat tcagcaaaga aagaaaagcc aatgctaaaa gggttcaatg caccaagtaa
     3181 caaagactta ctttaaaaat aaaaaggcga ggttacaata cctagccttt ttattatgta
     3241 caaatacaaa cataaacata tacgtataca tgtatataaa catgtatacg tatatatcca
     3301 taaacacgta tacgaaattt agttaagcta tacctaaccg cttattccaa taaaaaaagt
     3361 gatcagcttt aaactgatca ctcaccaaaa acttcatcat acaagttatc tatcatttca
     3421 ccaagtaaag ctgaatctga tcgaattcct aacttttcgc tgattttaga tagtttcttt
     3481 ctttgtgatg gttgaagcgt gaattgataa tttttcttct tttcagaact atcacttttt
     3541 agtttagcta ccaaagaact tgttgtttct ttaggtgttt gtttaggttc ttcaactgct
     3601 tcaaccactt caggaacttc ttctacttca gcttcagcga attttttaaa tctattttct
     3661 ttcacttcaa tcactcctta ttcttgtgcc gtatcaatta ttttgttgta tagcgtaaac
     3721 gtgctttcaa agaaatcctt attcttacga tagatattct tatccttagc gaactcagca
     3781 atattcttac cttctaagct agttgcatta agcaattcct tctcttgtac atatccaaga
     3841 taattagata acgttggtac caattctagg aactcacgac ttgaattagt attatgtttg
     3901 atcctgctac cgatcgtagc gtatttagtt tccatcagtg tttcaccagt cttaatatca
     3961 atcaactgtt ttgctagtgc atcaatatct ttaccaagct taagcaaagc ttcaaaacca
     4021 tctttagatg gatcagaaat gcctacgaca agatcagata cagcccaagc attttgagct
     4081 agtagacctg catcattatg agtatcaatc agaattagat catacttctc tttaagctgt
     4141 tcatagttaa caacgatcca tctaaacagt agggttctgt tgttaggcat atcacgcatt
     4201 tgcatctcaa cttgttttaa atcttcacct gctactaaaa tatccatgtt ctcatttata
     4261 gctaaaggtt cagctgatcc atgaagatat aaatgattga ttgtattctc tcttttcgct
     4321 ttactgaaag catcaggaaa tcgttttgat aggttttgcg acttatcttc atcaatatac
     4381 agcaccttca tacctaaatt ttcgacagca tatccaccta gattgtagat tgttgttgtt
     4441 ttacctgttc cacctttttc aatgtttcca gtaatgattg acaacttctt ttccatgatt
     4501 aaacctccct tattaatctt ttgtaactcc attatacact ataaacacgt ttatgtatat
     4561 atttatatac ataaacgtgt ttatatttat atttgctttt atattctaca acataaacat
     4621 atatatgtat atgtttatgt ttatgtatat attcaaatat ataactattg catttgtcat
     4681 acaatcgtag tacaatgtaa ttgaaaagga gttgataaac tatgccagta ataagtattc
     4741 gtgtatctaa agaagaaaaa gattttcttg aaaaagttgc tgaattcaac ggagataaca
     4801 tttctgaatt tatgcgtagt aattccctgc aaacagcaga aacgttagta gactttgcca
     4861 cctacaagca actaatgaaa gagcatgagg aaaacgatca aagtatttcc cacgaagata
     4921 tgctaaaaga gttaggctta taagatgact taccaagtaa gatatgaacg tgaagctcaa
     4981 aaagcgctaa aaaaaatgga taagtttcag gcaaaggtta tcctaaactg gatcgagaaa
     5041 aatcttgtag gcactgatga tccaagaaag catggtaaag gtctaacagc taataaatca
     5101 ggctactggc gatatagggt aggtgcatac cgtataatag ctgatattag cgagcatgaa
     5161 gtgacaattc ttatattgtc tattggtcac agaagtgaag tttataagta acagacaagc
     5221 tgataaggct tgtctgtttt tttgttaacc caacaaaatc actgtttacg atcccacttt
     5281 acctaaaaaa gaacaatatc ctcttgatat tatacacacc agtgtgtata atataagtgt
     5341 aaacaagcta attaactttc aaaaggagcg attttttatg ggatggtatg gtgattattc
     5401 atcagcaaat gaagtgatcg aagaaaagaa agggtacgcc aaaaatggtt tggaaatcgt
     5461 aggtaccaaa gtaacgaaga cctatggaat tatccttttc aaagatgcag aaggaaccta
     5521 cgatattgac agcttcatct ttcgagatga aatgtacaaa ccaatctact tcttaaatga
     5581 cagtcctgct tactttaaga aaattccgaa aaaatggttg aaattctttg tcggtgatca
     5641 agaaaaaatg gttaagctac aactagatag aattaaagaa gaaagcaata aaccaaaact
     5701 tgatgactta ttagttgcag gtaaaactta ctcaatctgg gatggatcgt atgtcgctgt
     5761 ctatcagtat aaactcaaac gtagctttat ttttcgacta caagatggat cactcactcg
     5821 gttcactcga ctatcagcag gagatgttaa ggaagtaacc taaaacttaa atacataaac
     5881 ataaacgtgt atacgtttat gtttatgtat atgtatatga atataaacgt attaggtacg
     5941 aacactttta aattcttttc tgggctttgt ttttagggta gggctaaaaa aaacatcacc
     6001 atctgatcca aaacagctca gaaacgcatt taaacgtatc tgataccaat attacagaag
     6061 gagtgtatcg tatgattaac ctatttcttt tagtgacagc tagtacatta ttctttactg
     6121 gaatatcact gataatttcc tatttgatct ataagaaagc cgaaaatcac acaagcaaag
     6181 caatcggact agccgtttca tcctttttaa ctttgctgac tttatttgtt actttatcgt
     6241 tttcttttgt ttagaacagc tgatccatta gcaccttttt ctctttacat tatacacact
     6301 gttgtgtata atgtatttgt aaacaagcta attaactttt aggaaggagt gacaagatgc
     6361 taacagctac tttacagcaa cctactcacg ttgctattgc agaaagttat acaattaatg
     6421 cagggatcgt taaacagctg atcaaggatc gagaattcaa aaagtttaac agtcggcaag
     6481 cgtataacag acagctaaag aaagctattc taaagcacaa agattccttc atcgggaact
     6541 tggaaagtgt agatgttaaa actgtttcaa cggcattttc agttgttgaa gtgactattg
     6601 acggtcgctt gatttctcta tttgcttttg gaattaattg atagggagtg tgaactttgg
     6661 aaggtaagac ttttgcagaa atttggactg aatttattga ggtaccaact cgcattgatc
     6721 cgataacttc tgaaaaggta ttggatgcag acttctacaa tctcaaagca ggtactagcc
     6781 ttgaatgcgt ttggatgtat ttagaagagc atttcaaagg cgtaacgctc ggtcacgtct
     6841 tataccacaa tggtgccgag tgggatgcaa ttggaaatat ccatttttct taatataaga
     6901 aaggagaaca caatatgaat tataccgatg aagaacaaat cgctttggaa tacgttttca
     6961 caaatgaaga aggggaggaa attgaaaaag tcttgatcat ttcatcaaaa gacttggaga
     7021 aggctttggc ttatgaacca tacgaatcaa ccgtaactgt cattgaattc ttagatacct
     7081 atacttctaa tgatacttgg gaaattatga atatttgcaa cgctaataac attcaatttg
     7141 ctgaaaaaac caataaatca ttttattagt tttgcaataa aacatcctct ttttctattg
     7201 attccataca caacaatgtg tataatagat tttgtaaagg gagggtagct atgcctaaga
     7261 cagcaagaga aattgtaaaa attctcaaat ctaacggctt caaaaagact ggacaaactg
     7321 gcagtcatgc aaaattttat aattccaaga ctaaaagaac ggtcgttgtt ccgatccaca
     7381 atggtgatgt accactagga acagaagcta atatctggaa gcaagcaggg ctttaacagc
     7441 cttgccttcc tatttggtat taggcatact atcctctctt acactagaaa aggagcgagg
     7501 aaatgaagac aaaaaatatt ttttatccgg caatcttcga gaaagaagga gaagcttata
     7561 acatcgtttt ccctgactta ccggaaatat ctacctttgg aaatagtctt gaagaagcat
     7621 atttaaacgc taaggatgct ttaggattag cgctatatag tgtaccagat gcagaatttc
     7681 ctaaaccatc agcagtggaa gaattaactt tgaaagccaa ccaagtggta gtaatcatcc
     7741 aactaaacat taaacttttc cgaagaagct taaatacaaa aacaattaga aaaaatgtta
     7801 gtgttccgga atggttagtt cttttaggga aggagaaaaa cataaacttc tctcaactac
     7861 ttcaaaaagc actggaagaa gagttgcttg aaaagtaaaa aggagaccga acctatgaaa
     7921 gctacacact taaactattt ctctcgaacg ttaatcaata atctattaaa agatacaact
     7981 ggcattagcg ttagtaccaa acactttgcc aatgatctaa ttatttttga ggataataac
     8041 ttgcgaattg agatgtttat tgaacaggca acccctaact caatacctga attctgctat
     8101 acgcacactg aaaaaatgaa acatgcaaaa tcagttaaag atgttgaagc attaagtgaa
     8161 tcactaacga acgctttaaa cctcgttaag aaggcaaaat tgttatttga atttcaaacc
     8221 gactaccaca atgagttgct tgctcaatct gaaattttag attaattgaa acaggagaag
     8281 agaaagatga aacaaacaca aacagaaacc gttaacgata cgcttgctga tcaattagca
     8341 ggttattata aattatttga gatgggtaaa gcacagcgct tgatccacta tctttggaaa
     8401 tacaaaattt tgtttctttt ttgggaatca acactaacat tctcagttct tttttataag
     8461 tcagattttt ataatcaaca cttagtttta gggagttttt tgcttatcta caccattttg
     8521 atgcttaatt tatataccta catcacactt gaagaacact tagtaattag cgatgcaggg
     8581 atcgagagaa acatcaaaag ggtatccttc aagatcacgt taaacaagcc taccgataaa
     8641 aagctctcag aagcgttctg agagcttttg acattttctt gtataatttt atcctaacac
     8701 attacaaagc acctaattcg agcttatatc aagcaaattt gctatttatt actcaataca
     8761 tatctaaaaa ttacttgatg gaaaattaga tacatggtaa gcatgaaaag aaaatcaatt
     8821 tgagcagatg cagtacaata catagcaatt taaaagctct cagaaggctt ctgagagctt
     8881 ttaatatatt ctttgataat ttgtctttct taaatccata cgtcctaaaa caagctgttt
     8941 ttgcttaata tgatcatctg agagacatca aatcaacttt tctctcaaca aatcattcaa
     9001 agctttcgac agattaatat tctcttcagc cgctaaaaca tttaaatagt gttccacagt
     9061 cactgatttt cgatcataat tttgcttgtt tcgagcttta gcaactttcg tattagcttt
     9121 tacgacaaat aaattaacat tctcagatcg cttgattctt gcatttaact cttgaacagg
     9181 cgtaggcgat ggaatttctt tttcttgcaa ctctaaaagc accaagtatc cttccaatac
     9241 ttcttgcgcc atctcaaaag catcttctaa agtttcacca tgtgtatata agtctggaaa
     9301 atctgggaat ttaactgcat attcatttcc tttccattcg aatactgcac aaaaaaagta
     9361 tttcatcgca ttgcacctct ctaattaaaa aataattttt gcttttttgt ttcaaaaaaa
     9421 agaatcgcct accaaaaaaa ttgataggcg atcacaagct aattaacctc taaaggagat
     9481 taataccttg ctcccaaggc actatatatc ttagcatacg tctgaagcat cttcaaacga
     9541 gtaagatata ttttgcatta acctaatttt ttttctctat aagttttttc atcaaactca
     9601 ctagaaatat taaactggac agcaaaattg taatttctgc cttttctaac tttagcaaaa
     9661 tcaatattca gcattgattt ttcattaatt tcctcaactg attcccttaa aacacgtcta
     9721 tcaaaattcg atattgtttt atactcattt tctgttttcg taatctcacg aattttaact
     9781 atttcaatca ggggattctt atatatttca gctttctcaa aatctccttt atttttggcg
     9841 ataagatatt ttttataata catcgccaaa tatttgtata acacgatgct atattttttt
     9901 tgaaaagatg ctaagtcttc aaaaacaaag aacggatact tctttttgat tgagacaatt
     9961 aagggaatta catcttctga aaaactgaaa gatacactat gctcttctaa atcccaatga
    10021 atccgatcaa tagcagatac agtagtatac gtcttatatt ttttgtctag attcgaaatt
    10081 tcataaactg cttgtctttg caaagtgtta actatggttt ttagttccgt ttcaagattt
    10141 tgatcaacat ccatgtaatt aataatcgct ttaacaggta aatctactac gcctgttttc
    10201 tttagctttg tgggtgagat ataactgaac agcaattgat aaagttcata cgactttaaa
    10261 tccattttca tgtcaattgt tgctaagaac tcctcattgt actttgtcag ttcatctttt
    10321 ttcaaaagtt tttccttcct tcacgcattt tttcagttgt taatcataaa ggttcacata
    10381 aggcaaataa ctcgtaattg atttttcgag atatttaaca gtatttttta ttgtgtaatc
    10441 ctccttatga gtagcaacgt aagataggtg attgacaact gattcaaatc ctctatcttt
    10501 ggataaacga tcataaagcg gataaacttt attctgaagt cggctcataa gtttaagatc
    10561 gcataagtcg gcagaactga tcaaaaagtt tttgattagt aatttagtgt atttgctatt
    10621 catagctttc aagtacgatt gattcatttt ctctgtttta tcttctgcgc cttttaaata
    10681 agcagggtct ctttgttctt gcttataagg taccggaact gacatggtac gtgtaagcaa
    10741 gaactttatg tttgtataag tatgaccctt cttgataagc tcatagtcaa ttttaaaatc
    10801 agtgtattta ttcagttggg caatcggttc tttaattaca cgtctttcaa agtctcgaaa
    10861 agctttatat tttttctcaa tactaaattt ctttcgtaaa tctgcaatgg agaaaacagg
    10921 attttttaat ctatcaagtg attttgtact ccatgttaag ttatctctat ttgcttcata
    10981 cagattaaaa tctttagtga ataattgaaa aaaactgatc gcatacttgc ttgtcaagtt
    11041 aactacttga tcaaatgtat attgcgtaaa atgatcttct aagttcgtta ataaaggcat
    11101 aattgaacta ctaaatttaa tagatactgc atcagaaaaa tcattgaatt cagtaatttc
    11161 tagcggtgag atgactttgt attcagtctt accgttaggt ttagttcttt gaattttaat
    11221 gacagattgc tgatgcaatt tttcaataac ttgttttaat cttgaatgtt ttgaagaaga
    11281 tgaactagaa gacatcaacg caaataaagc ctttttagat actttgacgg tattattaaa
    11341 aggcggattt ttagggtcaa gatgtcctat agcgtaaaga taaatttttt gtggcatagc
    11401 atccatttca acaatgctat ctacaagttg attcgataaa gtaacagtta aatcaaactt
    11461 ttttagattg ctttcggtag tttcaaattt cgggtttaca ttcttttctg ttattttcat
    11521 acgattgcct cttctttcta agcttggtac gagcttaaaa acttggaact ggtacgattc
    11581 cattgaataa gtatagacct gttttcgata cgttacaata agaaatgtat cgtaaattta
    11641 taacataact caatgtcagt aaaacacatt ttattttttc caatcaaatt tcattgaaaa
    11701 tacagcagaa atcgctattt tagaacattt tcaaaataaa aatccttttc aataaaatac
    11761 gaaattataa aaattaccaa aattaaaaaa caaatatttt tctagttttt agaaataaat
    11821 tcacatactt tttctttttt tggatatatt cggaatacca catcgctttt tgtccacgtt
    11881 tgcatcgctt tttgtccaca ttcacatcgc tttttgtcca tgttcgcatc gctttatgtc
    11941 caccctaaaa tctatttaag attgatataa agcctttttg gaggttgcta aagttttaaa
    12001 gattaaaata tatattataa atataaataa aaggggaagc tgaagcttcc tccttttcct
    12061 ttttttcaat tattcaaagt tacacaagca aaataaactc ctagatagtg ttagtttttt
    12121 taagattttt tatttttaag attttgaaat aagagaggag taatcctcct ttattaaata
    12181 tctgttaata tatctgttaa atatctgtta atatatctgt taggggaggc ttaatctctt
    12241 gggagagtaa gtattaaagg ggtataaagg taacactttt ggacagtaaa ggtaacaaaa
    12301 gtggacagta agggtaacag ttttggacag caaaggtaac aaaagtggac atttgaggta
    12361 tcacactagg ataagacgtt accttcggtt ttcttgattt ggaaggcgaa aaaatagaaa
    12421 attagattag taacaaatct ttgttgactg ttacctttag ttgtgattta atttaggtaa
    12481 caagcagaaa aaattgtttc ctttaattag taccagatta acatgattaa aggagcgcaa
    12541 aaagaagatt ggagagtaat caatgtctca agaagttacc gaaaagatta ttgatagtga
    12601 tgtagattta gtatcagaac aaacaaatgc aattgtgcgt tataacaatg gtctgaatac
    12661 aacagctttg cgaggattta agcctgttga aatggatgtg ttttgggcta ttttatcaaa
    12721 aatgaaacgt caaggaacag cagaagtaac ttttgatttc aacctctttc gttatttggt
    12781 caaatacgac agaagagaaa aggacagttt ttattctgtg ttaaaagatt tgagtgacaa
    12841 actaggtaat ttaacctacc gttttgaaga ttcagacgtc tatgaacagc tgtggctttt
    12901 tcaaaaattc cggatagata aacagaagga aacagttaca attaaggcaa atgaggaatt
    12961 tgaatttatc ttaaactcaa ttggcaaaaa ctttactcgt tttgaacttg ataattttac
    13021 aaaactaaga agtagctaca caaaagaatt gtatcgtcat ctcatgcaat acagaaataa
    13081 agagtggcga aatggttatt gggtagtcaa agttgaagat tttagaagag cattaagtat
    13141 accttcaagt tatagaatga caaatattga taaaagaata ctagagcagg cgaaagaaga
    13201 atttttagct cctgatgaaa acggaataca ggtatttgaa acttttgatt atgaaaaaat
    13261 ctatgcaaag aaaggaagaa gagttgatcg gctcagattc acttttagtg agccagaatc
    13321 aaacttgcca accatttcaa tgcataattg gttagaagaa gattgagaag aagcaggttg
    13381 taattttaat acaacctgct tctttaaaaa agtatgggta acacttttgg acaataaatg
    13441 caagaagttt ttttgtaagc aagtttcagt ctaaataata tttgaaagaa tggctaatct
    13501 atacaaattt taacaactat gggtaacact tttggacaag tcaagaaaaa aaacaatttt
    13561 tcttaatttg ttgatcgact aaaggtaaca cttttggaca acacagaata aaaagtccgg
    13621 tgatatcagt tttggtagga aacatcttaa tatctttgtg agattgaagg taacattttt
    13681 ggacaagtca actatttgtt aaaagttaat atagagtaat tagataagtt ttttcttttc
    13741 gtctgataaa tggataatat tagttatttt attgctaatt agtctttcat gatgactgaa
    13801 atagctaaaa taaatatctc gttggataat tgctgatact ttaagggata tctaaatttg
    13861 ctgattgatt tatttatgtt attatgatat tataataaca taaacgtgtt acgcccgaac
    13921 ctcattttaa aaaaatgaag gggtgagaca atgaatgcta ttaattggca tgaatttgtt
    13981 aactatatgg agagcttact agatgatcta aaaagtttta gtattgctga tgcagtaaag
    14041 catgggttac cagttagtac aacgatgaaa ttacgaggat tgaaaattcc agatgaaatt
    14101 attaagctga gaactaaaac aattattgaa accgcaaaag tcgttgacaa tatgaaccat
    14161 gaactaaaag gcaagaaaac aacttacgag aaagattttg aacgtttttt aaaatctgtt
    14221 attggaaaat atctaggtaa aaaatctttc agtgaattga gagatgaaga gattgtacct
    14281 actccaatgg ttagcaaaat ccgaaaaaac aaagccgagg ggaattacta tcttggtaca
    14341 atagcaaaaa tcattgatta tttggaaaat aaagagcaaa agaaaggaac gaagaaagga
    14401 agataatttt tgtgttaaat attaatgggg aagaatatac cgaaatgatt caaaatcccg
    14461 aacctagaat taccgaaaaa acgtctgatt gtttgctctt ttatgaaagg gggcagacaa
    14521 actaatgaaa aaaaacgtca attcaacatc taccaaagat caatacagta ttattggagg
    14581 gttgcttggt gataaggata agagaacacg tcacggatta cgcattatgc ttgcaacttt
    14641 tctaggagtg ctttttttat taacaggctt gggtagctac atttatcaat taggaaacta
    14701 taattattat ctcgtgaaca atacaacacc tataggctct tcattggagt ttgctcgatc
    14761 aggagcagag atcactattt cagatgtgtg gacagatcaa aacagagatg taacagtagt
    14821 aaaactcggt tactcgcaaa acgctagaaa gttgttatct acgcaaggaa aaaactacaa
    14881 cttatatatg gcaacgaaag aaaaagatcg tcctaaaaag cttgaaatga gctatgggat
    14941 gttaagtact gatggggatg ctttcttatt cctaaaaggt aaattagatg aaagagctta
    15001 tcaagttttt attgcaaacc aagtatcact aatttctggt gatgatacag ctgacgatga
    15061 taccaaagtc aatgtgaatg aagcagattc agttacaaaa gcactttctc aatattctat
    15121 gaatgatgtt aataatgaag gagtaatttc ttttgggaaa aaagcaaaac ctgttaatac
    15181 tcctgataat atcaatttca gaataaatcc atattcagat tcaactaatg tctatcaagg
    15241 aagctttctt accaaagatg gcgatattga ttattcaaaa gttgtagcag ttacaagtaa
    15301 gaataatgtt gtaagtaagt tagaagatca gttaaatgct tcaaatacta acatcaagaa
    15361 aattgaagct acaaaagctg agtatctgga acgtttacaa caaaatcctg acgataaaga
    15421 ttcgcaagaa agtattcgtc aactagacag tcgaatggag accgaaatta aagcaatcaa
    15481 tgatacacaa aaattgattg atcaatacaa aaatgctgac tttaacaaag attcttttgg
    15541 agacatgaag acgacattta agtacctaac tctgactgac tagaagtaaa ttattcttat
    15601 tttagaggaa gtgaacacaa acagatggaa aatagtaccg agttttttaa gaatattcaa
    15661 actgaattta atcatgctct cacaccaatg aaaccaccga aacataagtt agtgggacgt
    15721 gatgacgaac tccatgagtt gtcactgatc ttgaatagac cggaaacacc gcaagctatc
    15781 ttgttaggtg aagccggaac aggtaaaaca gcacttgcaa gtgaatgggc tgaaagacag
    15841 cgacaagatg gaaagaaagt acaagtatac aaattatcaa tcggacaatt gcgtgttggg
    15901 ggagatgatg tcctccaaac acgtttagaa acattaattc caagattgaa agaatatgag
    15961 cgaattttaa aatctgagga caaagaaaat gaatttgtcc tcttcattga cgagtttcat
    16021 aaagttgtta gctcttttgg tagtggcaat aaaattgggg gtgattcgct aaaagaaccg
    16081 ttagcaaatg aatcacttca attgattacc gctactacaa gaaaagaata tgaaaactat
    16141 atcaaaagag atgatgcctt agggagacgg ttacaaccgt tagaattgaa tgaactaaaa
    16201 agcgatgttg tcgtcgatat tttgagacaa tggctaaata catcctctgt taaagatgat
    16261 cctcaggctt cttttaaatt atcgcaaagg attcctgatg aattacttca agaaatcgta
    16321 caagcaaaca agctttatcg tgaggatttg tccgaaccgg ctaaatcaat tgccgtaatg
    16381 gaggatttga ttgcaaaaga tcgagtagac ggaaatgctt ataatcgaga aagtctattg
    16441 caaatatttg gtaagtataa tattcagcta gattctgatt ttgatatgga aaaacttcaa
    16501 gaggaattaa agtccataaa agggcaaccg ttagcagttt atactataac aatgttgttt
    16561 aaacgtatga agcttggatt tgatcgtttt aaaggtcgaa agcaaccaaa agcaactgct
    16621 ctgtttacag gatcaacagg ggtcggtaaa actgcaatgg ctaaagccgt ggcaagagga
    16681 ctttatggcg atgaaaataa agttctctta gttaatatgc cggactacaa aacaaaagat
    16741 gatgcaatgc gttttagacg actcataggt actcatgtaa ggcatgatcc ttctgcgatt
    16801 attgtgtgtg atgaatatga gaaagctggt gtagaaacac tcgatgcctt tttatcaatt
    16861 ttagatgaag gtaggataag ttttactgaa agaggatggg atgatcgaga agttcacgac
    16921 actgtttcat tgtcaaatac aattttattt gcgacaacaa atgcaggtgc ggagatgttc
    16981 gattcgttaa acagatatag ctcactatct cgtgttacaa acaaggaaaa ttctaatgaa
    17041 tatagcactg aactaaaaag agaataccga aaagtcgcat cagggatcaa acaagccttg
    17101 attggtgaag gtctaaagcc tgaagttatt aaccgatttg gacgaatagt tccattttgg
    17161 gctttatccg aacaaactct tctggaaatt gcttataatg aaatgttgaa gagtgctgaa
    17221 ttagtacgaa aacatttaaa tatagattta cgtcttccac ctaggaaaga aagagtatat
    17281 ggtcaaaatt tttatgcaga cgatctctcc atgcacttag tttttgacat ggcttcacaa
    17341 gaggattcta aatcaggggg tgcaaggaat atcttgcatt tagttgaatc tgagtttgaa
    17401 gaagcgatta ctgaagcaat aattacttat ccaaacgcaa aaatattcga tgttaagttg
    17461 agtgaagccg gagcgtttga aggaaaaggt gattctaatg ctgaaattat tgcaaagcct
    17521 tccgttgctg aaagtaaaga aaacaagatt gtattttcga ttggaggggc gtagctatga
    17581 aattcaagaa atatttacta caaaacctta gaataaaacg atgggtcgta agaggtctag
    17641 ttattggagg agtattagca gttactttag gcgctccttt tggaatgaat gatccaactc
    17701 ctgacaatca agatagcaaa caagaaacag ttataaataa gaacgttgca tatgcggaaa
    17761 gtaaaccggc agaacaagga atgtggacac tatatgctcg tatcctaatg ggagataaag
    17821 aagttaatga tagtgccgct aaaactggac aaatgaattt gttaggacaa ggtggtatca
    17881 gtgccgattt cccttattct gacatttcta aacaaggtaa ttcgattgca gggggtaatg
    17941 caggtactca actagcatct actttagcaa cttatagtta ctacggatat attgaaacag
    18001 tctcaggaaa tgctattgcc agtcaagcat catccattct aggaagtatt ggtaggacga
    18061 ttggtagttt tatcggatca attagtttaa tcctttctaa tattgctgaa tccataaatg
    18121 aactccttgg aaaatttttc atgagtatga atatattttc tcttatgggg tggggagaag
    18181 gtggatcgga tgtatctaat ccggtatcta aagcattaac tagcttgctt aacaatttgg
    18241 gattaaataa tgaattattt aaagcgatag caagcttatc tatgctcttt ttcacagcaa
    18301 tactaggtta tcaaggaatg aaattattgt cgaaagatgg tgttaactcg aagggattat
    18361 caccagtact tgaaaaatgg attgttcgta tttttgtatt gtttatattc ttaccagtgg
    18421 caggatcgct attgggagtt tatatcaacg gcatccagtc agcaaaaaaa gatggagata
    18481 taccaacaaa tgttgtttct gactacatca ttaacacaag actttgggct tctactcaaa
    18541 atcttgctcc atcatctgat actggtggtg atgtacctga cggaaagcct acatccgaaa
    18601 tcaagttcgt tgatacagat tatgaaccat ctaaaaaatc tgcaatgata agagcgatca
    18661 atactagcag ttatgccaga caaaataatg atctagattc taattcagtc ggtatggctc
    18721 tattaaaaga atggtcatcg aatgatactt tcaatattaa tacttatgcc ggtgatgtta
    18781 atgtcgccga aaatgatgct tatatgggaa gagcgcctgc tgtttggaca ggctataatt
    18841 taggggatgg aaaagaagga agtgcaatta agaaaccaac caatttaaat ggatatattt
    18901 ggtcagcaaa tcaaaatgtg acagacgaca atcgcaagcc tgatggaaaa ggaacagcgc
    18961 ataactttga tgctactcaa ccttggggcg ttgctaacaa tgcaactttt agcacacagt
    19021 cggtagcttt attgctccaa tcggcacttg atacatcggg agctaagttt tatgcgtata
    19081 atatcgctcc ttcaggaatt caaggcagtg ctaagaatat gtcaacagta aaaacagaat
    19141 ggcgtacagc atccttagtt ggtcatggag ctatgggtaa gtttggctcg tggctagcga
    19201 tgaacgctga atcaatcgtt ttagcagttc tttacttagc atgtgcttgg gcgatgttca
    19261 atgtgaatat cgtgaaagca tttaaacgtg aatttattcg gatttgggaa gtagttctaa
    19321 caggatcact tgcatcagga gcaggtgtga tggcaatttc attaggtgtt gttacaacat
    19381 ctctgatcgg actacaatta cctatctttc tgattacttt tattcgtgca tttggtgata
    19441 ctgctgtggg attcattcct aatacattag ttggcgatgg cgtagcaaat attgttcgat
    19501 ctgctatgat tttaggtgta ggatatcttt tagtctttaa aaaaggcgaa aaagtaaata
    19561 atttgattaa tatgcttatt ggcttaccac ttgaagtagc gtttgggttt gatgaacgtg
    19621 ctaagttatt atttggtaat agaaatgatg ctgtgcgagt tgtggacggc gtttctaaag
    19681 caatcaagaa aagccaagga gctaaaaatc cctattatga tggtggcaac agctcagtaa
    19741 aagatatggt tgcaggaggt cttggagcag ttggagcgca aaaaatggct gaacgtttga
    19801 acaatatggg agatagcaat gctgatgcta agtccattgc tgataaagta attcctgaaa
    19861 caccagaagg aatgtcattc aacggtggcg gagttggttc cacaatcaat aatacctctt
    19921 tcaaagattt agttcgtcaa gatcaactac gtaataactc ccaacaatat attgatcatt
    19981 taggacgtat ggttgatgaa aacggaaact ttgtcgatga aaatggtgaa atattaaaag
    20041 atagtaaata cggtaaagat gctaaaggca actttattga cgaaaaaggg cgtttggtcg
    20101 atgaagatgg aaaccttata gatactaatg gtaaaccatt ggaagatagt gaagatggta
    20161 atattggtat ggatagcgaa ggacgtttaa ttgatgaaaa aggtcgctta gtcgatgaag
    20221 atggaaatct tgttgacgaa aatggacgca ggttgctaaa tggtgatttt ggtcatgata
    20281 gtaacggacg tttagttgac gaaaaaggtc gtttagttga tgaagatgga aatcttgttg
    20341 atgagtttgg taataaactt ggtaaacaca gcgatggata taatgtgaat ggagatttag
    20401 tcgatgagga tgggaatatc attgagacga gagatgcgct tgtggcagat cgaaatagag
    20461 aactcgaaca acaacggcat acgccaattg ctgttgcaat tcctgagaat gaagaagacg
    20521 agattacttt agatcaaact catcaagaac tgaggaatga aatgagtggt gaggacgtgt
    20581 cagacagagt gcatcgggaa ttaggagatg gagcatctgg tgaaagtcag tcaagagagc
    20641 ttaacagcaa ctttgataat agtgctattc ggaatgacac agtacttaaa gacagagaat
    20701 cgctaacaaa agaaacggat aaagaacaat cagatgtgaa gaaaaagttc attcctactc
    20761 ctaatgttaa tcaaacagat aaatatgaaa caaatattgg atcaggaatg tcgaaagaca
    20821 atcaatcaat tagagaacct gaactgacaa ataaaaataa tagaacaaat aattctaaat
    20881 ctacttcaga taattcaggg aaatctaatc aatcaaccaa gaaaacatca acagctaaat
    20941 cagccgttga atatctatca aaaactggta aaaatgttta cacctcagca gataaagctt
    21001 tagcaaaagc tccaaaaggt agtctagcag ggataaccta tgaagctaaa aacgaagcta
    21061 ttaaaatagg taaaggaacg gctaaggttg ccaaggctac tgctacagga gcatccaaaa
    21121 ctggtaaagt agtgaagaaa tctaccatgt cagtcaagga ctatattgta aaatacggta
    21181 atcaaaaatc aactgaaaat aagtctgaag acgagtaaaa agaaggagca ttgatttgct
    21241 ccttcttttt actttgattt ctaactgaag atgtcaagga aaatttctat ttatgcaaaa
    21301 aaattatttt aaattgtcta taaatgaatt aaatatttac tgttcaaagt tgtataatta
    21361 aaaagggctt atgctggtaa agaacagaat tattatctaa acaaaaactt gataaaccag
    21421 catttttagc caataaaaaa agtgaaataa tttaatggaa aatctctttt aatcatcgaa
    21481 aatttatgtt attatgataa cataaagatg taaatatcga gacgtctttt tcttagttag
    21541 atcattgaca agtgaacaaa taagatttgt cgatgatata attaacttat taaaaatatt
    21601 aaaaaggagg tttttatatg ttcgattcta ttaaaaattt ggttagcgga atgacgcctg
    21661 atatgaaaac tttagtacaa gttgttttgt atttcttggc attcgttttt tcgttgaaac
    21721 caggattcac tgcaatgaaa ttttttggtg atgcaaaatg gggagatgga ttaaaatatg
    21781 ttggggcgat tattgcaatc tttattattc ctgtaattac aaccgttatg ctttacacaa
    21841 tgggtactaa aacaggtaat gatttgaacc aaaaagctaa tatcatatca gcacttattc
    21901 cagttgttgg tatctatcta gtttcaaaat ttccaaaaaa tgaactgtta ggttaaaagt
    21961 gaattgaaca aatttggata atatgccgat ttgttcaatt tactttttac caaaaaacta
    22021 cgctccaagc gtagtttaga agtgagggaa gaaagtaaat gtaagatggt ttttgataga
    22081 gaaaaaatgt tagctcacgc caacgaagta ttaatgagca gtctgaaagg taccgaactg
    22141 gcaaagataa tgaatatgaa tgttaaccag ttctatgatt atcgaaatgg gagtaaaaag
    22201 attgaaaaag ccagacttga aacgcttatt aaattcgaaa aagcatatgt atatatgcta
    22261 gataaacaga aaagaactat tgactgaaaa aaggagtttt acaataatgg caaaattcaa
    22321 agatattacc gaatctatta attatgtggc agaaacagtt cctgtagttg cactctcttt
    22381 gaagccacaa ttactcgaag tgtacaaaaa agaagaaaaa gaagcgaagg caaaaaaagc
    22441 taaagcaaag gtatggaaag aattcgatgc gttgctgacc aaagatttac caaagaaaaa
    22501 ttatatggta attgaagatg tacaaaagct caacagtgag ggagttatag atgaggatga
    22561 tgctattctc gattctgaga agtatgtcgt tctattgaag tcaagtttga attatgtagc
    22621 tgatctacca gaaaaattta aagagaagct attagcaggt gtccgtttta tggatagtag
    22681 tggtcaatta ttagttattc cggatgatcc taatgtgttt gaatcagaag ttgaagaagc
    22741 tacagacttc tatagcgcag tagagcaatt agaagtagct actaatggat tagaacgaat
    22801 tgctgagacc ttcaactctt ctgaagaagt tgaatttgaa gccgtagcag aaaatgtacc
    22861 agatgaaaaa gaagctcttt ttgtggatga taacaaacca accgaagcag aaaaccaaga
    22921 agtaagcata gaagaaacac aaatacaaac agaaacctac caacaaccaa aaattgatga
    22981 aaaacccgaa tcccaaagtg acaaagaaaa ttatgtttat gaagctgaaa aaatcacatc
    23041 tgaaagtgat gtaccgaaaa acttagatgc tgatttttta gaaaaaaatt ttgaacagat
    23101 cattgctgac aaatttcaac ctgtagtttt agatgagatt aatgaccctg tcgatttaaa
    23161 cgaggagatg ctatccatac ctgagttgaa accattgctg tttttagcta aagagagtat
    23221 gaacgctcgc atctctattg ctaataataa aatcaacagt gaattaaata agacgaagga
    23281 gcaattgatc ctcaaaggaa agcaaatgtt agctgatgag cttttgcgga tagaggaagc
    23341 aacctcatta aatggtcaaa atcagtttac aaatacacta tcggttgcta aagagagcta
    23401 cgaagcagaa gcatctaaaa ttgatgattt agtgatggaa aaatccaata tcattcgtaa
    23461 agattatgat atggcgaaac aggcttattt ggatgctgaa ctaaagaaac tagagcaacg
    23521 gtatgatgct gagcatttac cagaagttaa acaacaagaa gatgaatata gaaaagcaac
    23581 tgaagaaagt cttgatcaac agtaccaaga agcaattgaa tctattaaaa acgatgcatt
    23641 gaaacaaagc caagaagcaa aagaagttgt ggtggaacga attatcaatt cattgaaacc
    23701 agaaattgaa aaatcattaa cagacgtgca agaaaaaact ttagagattg tcgaaaaaac
    23761 aaagtcagaa aatgaagctg agttccatga tacaaaagag aaaacaacag atgtggttgt
    23821 caaatatttt gcgaacaaag aagaagttga aaagcaggta gctaacaaca cgaaagaatt
    23881 agaaacaaaa cttgtagagc aacaaaaaac tatcgaagaa tataaagatc gaatgaaaga
    23941 ggtcaaagat ttagcagagg gttataaatt aaatagcgaa aagttggcag aacaaagtga
    24001 agctttaaga gaagacttat ctcgttcccg aagtgatcta gctcaaatgg cagaaaaaac
    24061 gaaatcatta gcagttgcta cagaaaatca atcaagttca aaacgtgatt ttacaagtaa
    24121 aaagatggct actatcattt ctgtcgctgc aatggcttgc ggaacgatct tgggatcgtc
    24181 ctactttatt agtcataata atcaacaagc acaggctcaa actcaaacat ctatttcgag
    24241 agtggaaaat tcgattggga caaatacgaa ctctgcttca acaaatagta gctatgaagc
    24301 aacaccaact acagagttca aagttgggaa agatgttgtc atcagtattg atgggaaaac
    24361 aactaaggct gaagtgattt ctattgatgg agaaaaagca gttgtgaaaa ctccagacgg
    24421 aaatcagtac aaaattcaag ctcacttatc tgataataac taaggagggc tattatgcga
    24481 gaagaaaaaa tagtttatcc agtaattcaa aacaatcaag aaaacatgac ctcttctcag
    24541 cacttacagg aagcacagct cgtgtatcag gtaaatgaac taccacaaac gaacgagaca
    24601 atgagcaagt caatatcttt aattggcttg cttattgttg caatagtgat aactgtaggt
    24661 gtttaccgta agttgaacgt gaaaggaagt atttctgatg gcagataact caaactatgg
    24721 atttaaacat aacttacatc aaaaaccttc tgaaacaatt gtcagagttg aatggcttga
    24781 aaagcatgtc aatagcacct attctgttac agcaaaaaga ttaattgtat cagctatagt
    24841 tgttgcagtc gttgttcttg gatcaattgt ttacttagtt tctgatacga ttaaactgaa
    24901 atttattttt gttccaacgt tattaatgat aattggattt cttattctaa ttagccagta
    24961 tatgtttatt gatccatcta gtggttcatt gatttctggt tctattagac tgttcaaatc
    25021 agtagttaat tcagcaagag tatctatggg gaaaaagaaa ccatttcgag gcacagagat
    25081 tttaaaagtg aaacaagatg gaacaatcgt atatcgagat gggagtgtag cattaatgtt
    25141 caatctagat ggggctacat cactaaccgc ttttccagaa gaagttatgg ctcaggaatc
    25201 gaaatcaata caatatcaaa ataccagaga tcgttcctta gctgaaatca aaatcacttc
    25261 atctcaacct cagaatacgg aaagacaaac tcgaaatatg aaaatacaaa aacgaaatgc
    25321 aaggagtaac aaagcagtct ttgacataat agatcagcaa gaacgatttg tatcagaata
    25381 tattgatggt gtgaaaacaa cagtaatcca atatcttatc ttgaaatctc aatctaagat
    25441 ggtattggat gaaggtgtgg aacgtttaga aaactatacg agtagaggac tttattatag
    25501 tgtcactcag ttgtcagcta aacaaacaaa agaaatatta tctgatattt atgcactgaa
    25561 atagaggtga gagattaaat ggaaattatg ggtagatcac aactattaaa tttcaaaaaa
    25621 aagagaaaaa ctaaaaaaga acaaaaaact agaaattcaa aggtagtgaa taataccttt
    25681 gttagtgatt taacatcttt agttggttat caatttacac catcttacat taggacaggt
    25741 aagcggtggg gaacaattgt gaaattagtg aatacttatg gaatgaatag aaattatgga
    25801 tttggttgga tggttaaagc tattcctgaa gtgagagtag aaggggtaaa agcctatcta
    25861 attgaagcag ataagccagt gtcacaaaaa gatcaacaag aaataatgca cgagaatgtt
    25921 gggggatcaa cgaatagtta tgaaaataat ttagaaggaa aaaattcaga tgctgaaatt
    25981 agcaaaactg ttatgcggat tatggatttg aatacagcat ctttactaga cagcatggca
    26041 aataaaatta ttgattggaa gctagaaatc ctattagtat ccgattctcc taataaaatc
    26101 agcgaacagc taattaaact ccaacggttg tatgatgaaa aaatgtatgg attaaagctt
    26161 atgtcatatg gaggagatca acgtgatctg tttgatagac ttttaagtga accaatcggg
    26221 aatgtctatg agtatacgtc tatgagttcg gattttgcag gaaatgatca tgcaattcgc
    26281 aaaggtttag atgatgcgaa tggatgggct atcggcacta tgtctactag ctacgcaagt
    26341 ggtgtagctt ttatggcact ggatgattct tttacaagta aagttttagt ggcatcacac
    26401 aaaaacgcta caattaaagg ctatgatgaa aaaatctcag catcatcact ttgggggcaa
    26461 ttgattgcta ataatgcaat ggcaaatcat aaacgaactt ttcatattgt cttaaataaa
    26521 tttgattact ttggtgatcc aactggtaga acatttgttg ccaaaccttc gttaaacagt
    26581 gtaatagaaa aggttaattt ggctgaagaa ggtcttaact ctttggagct ttttggtaat
    26641 caaaaggaca ttggtgattt atatagacgg aatctcgata aactaactca gattttttat
    26701 ttgttagcag gcagaacatt agattctgca actagagttt tattgaaaaa agaattaggt
    26761 aacttttata taagtcagaa actttgggat attaattatg aagaacgttc aaaacttttg
    26821 gtgaacagta gtgatacaat gccagttctt ggggatttta ttagctattt gactaatgct
    26881 gaaacaaaag ccaaaaaatc tggtactgaa aaagaagtcg atcgtattat tgtattaaaa
    26941 gacacattga gaacagcatt agacagcttc tctaggatgt ttaatcaaag aacgacactg
    27001 aaagaaatcg gtaaaagtga caaattacag ttttactata acttaggtga tctaagagca
    27061 gaaaccaatg ttatggaagc tcaattttta aatacctttg atttcatcac atcacaagca
    27121 aaaaaagatg atattgttat gattcacggc ttcgataagt tatctattga aaccgttggg
    27181 ctaattaaaa aaagattaac aatgcttgaa gaagatggaa ttcgtcttgc ctttctattt
    27241 gatacaatcg gttcaggaga agacaatgaa tacaaagaag atattgaaac agctgatata
    27301 ttcaatacag atggaaaaat ttaccaaagt tttgaaagtg attttaatta cactatcatt
    27361 ggaaagatga ccagtggaga gttaatgaag tatgaaaaaa aagttgctcc tgctagaaaa
    27421 ggagaagatg atcaaaaact ttctgttgat ttgagacagt atttgacaac aggaagaagt
    27481 aataaatttc agatcaggag accagtggat ctgactaata attttgttat tggagagttt
    27541 attctttaag ttttatcttt gattttgagt gtataaggaa aggataagaa aaaacatgta
    27601 tatttgagac taaaaacagt cttagagaac atggtacgtt ttgtgaaaag agaactatag
    27661 aaatttcata aagtgaaacc ttattgagtg aaataattat agtggaggtt aaataatgaa
    27721 accaaacata aaaattttag atcgaatatt tctaggtaga gacacagaag taatcctaat
    27781 tcaacatgaa gaaggatttg aagttagtat aggtattcag aaacttcaaa aacctcatta
    27841 ttgtaatcag ttgtataaaa actttactga tgaagaaaaa gcgagagttt tttttaatga
    27901 agtcaaaggt atgagagaac agtatgaggt ggttgaagca tgaataagtt accagaaaag
    27961 aaccgtgtat ttagattgaa aggtgatctc agtcaattta aaatagatga gaatggacaa
    28021 ttttttgttt ggatgcgaca tgagcattgt tggtgtaatc cttttgtgtt aaaaaaagaa
    28081 aatgaaatca gaactaaaat tgcaaatggt gagtgggatt acctcttcag atgggaagat
    28141 gaatctttta tagcaaatga tatggatcat gcatttgtct catttctgga taatacatgg
    28201 ggtacttggt acgattgtta cctagaaaaa ttgggctttc aaaatattga tattgatgct
    28261 attgaatgtc ttaaagaagt cgatcaaaag tatgaagaag aaccctatta tctcttgcaa
    28321 caaaaattag ccaataattc agtgtttcag gtagaaattt gggaaaatga agatggaact
    28381 tatgaggggc tagcgcaaga aatacccttc tcagatagga agagttcgat ttgaaaagag
    28441 tatatcagca atttttatga agtgaaagca agcttacaat atagtaatgg aaaattgtgg
    28501 ttagctggaa ggtatattcc agctaaccaa atcttacgtt ttctgggaga gtggcgtttt
    28561 aaaacaattt cctaggaaaa atagttaaca taaaaggaat gaattgttat gaaacaatat
    28621 ttaaatgaat ggaaagcgat agaggggtca ttagtaacgc aaagaataaa ggaattacct
    28681 gattgcttag agaaggatca tttgtttcaa atacgagaaa tgttgaaaaa agaacagttt
    28741 gatccagacc aatttttagt agtcgagtat ccaactattg gagtgtactg ttgtaaccat
    28801 atcaatgatg gaaaatattt tattattcag gagtatggag gacagttaac tcctttctat
    28861 actacatggg aaatgagtga agaaggaatc aataattttc catgtgaaag catcgaagat
    28921 tcaatttcac acaccgaatg ttaattgatt ataactataa aaaaggattg aacattatgt
    28981 tcaatccttt tttcaatgaa atgttttccg ccatttcatt gtttggtgcg atttctattt
    29041 tattatatca aaataaagag agattctaaa gaaagaagag ggaaatatat aaatcaagcg
    29101 acagtatatt tattattaaa tgctattgtg agaatataac taattagttt aatttgaaag
    29161 taaatatacg ctataaaagt gcatcttgtg ttataaaaaa ttattttatt ttgtgaacat
    29221 aatttaacaa ttctttctcg gaattgattt cttttcttaa atagtcttca gacttattaa
    29281 taatattatc tattatgtct agatacatat cacttttcga ttcagctaat tcatacagtt
    29341 tttttaattc tttatccttt aattgaaaac cctgatccat agtcactgca tttggatata
    29401 gaagcccact ataatgttta cgtaaattca cttggaattt ctgattataa aagtctaaca
    29461 gattagaaat aaaaggcgga aaattactgt ttttgtaaaa ttgattattt ccatttaggt
    29521 tgtatagtaa tttgttaaat tgctcctgat ttaatttatt gtaaatccat ggtaatactt
    29581 ccatatcctt atctgctaac ctgaatgaac tagcatcggt aagatgttcg atcataaaat
    29641 ctatgaacat ctctttttca ccaaaatata aatattgatt atatattgtt tctaatgttg
    29701 aagatggtag cgaagaaact ctcatttttt gctggagtat aggatcaact agactattta
    29761 agtgattttg taattcttca ttgttgtttc ctagaactgc aagggaacgt aaacggtagt
    29821 aatattctat ttctgttagt acttgttgtt tcggttt
//
LOCUS       DACDIP010000029        28349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDIP010000029 DACDIP010000000
VERSION     DACDIP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.029"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IV257_001490"
     tRNA            8..81
                     /locus_tag="IV257_001490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IV257_001491"
     tRNA            101..173
                     /locus_tag="IV257_001491"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IV257_001492"
     tRNA            201..274
                     /locus_tag="IV257_001492"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IV257_001493"
     tRNA            288..361
                     /locus_tag="IV257_001493"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IV257_001494"
     tRNA            385..474
                     /locus_tag="IV257_001494"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IV257_001495"
     tRNA            487..560
                     /locus_tag="IV257_001495"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IV257_001496"
     tRNA            579..654
                     /locus_tag="IV257_001496"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IV257_001497"
     tRNA            656..726
                     /locus_tag="IV257_001497"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IV257_001498"
     tRNA            740..813
                     /locus_tag="IV257_001498"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IV257_001499"
     tRNA            838..925
                     /locus_tag="IV257_001499"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IV257_001500"
     tRNA            947..1018
                     /locus_tag="IV257_001500"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IV257_001501"
     tRNA            1041..1130
                     /locus_tag="IV257_001501"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IV257_001502"
     tRNA            1143..1216
                     /locus_tag="IV257_001502"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IV257_001503"
     tRNA            1235..1307
                     /locus_tag="IV257_001503"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IV257_001504"
     tRNA            1311..1383
                     /locus_tag="IV257_001504"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IV257_001505"
     tRNA            1388..1458
                     /locus_tag="IV257_001505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IV257_001506"
     tRNA            1471..1544
                     /locus_tag="IV257_001506"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IV257_001507"
     tRNA            1556..1627
                     /locus_tag="IV257_001507"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IV257_001508"
     CDS             1893..3086
                     /locus_tag="IV257_001508"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8814626.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IV257_001509"
     CDS             3216..4688
                     /locus_tag="IV257_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8814627.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IV257_001510"
     CDS             4771..5559
                     /locus_tag="IV257_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8814628.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IV257_001511"
     CDS             complement(5626..6294)
                     /locus_tag="IV257_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8814629.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IV257_001512"
     CDS             6409..8121
                     /locus_tag="IV257_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814630.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="IV257_001513"
     CDS             8124..9893
                     /locus_tag="IV257_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814631.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="IV257_001514"
     CDS             complement(10098..10664)
                     /locus_tag="IV257_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8814632.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="IV257_001515"
     CDS             complement(10664..11620)
                     /locus_tag="IV257_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8814633.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="IV257_001516"
     CDS             complement(11620..12276)
                     /locus_tag="IV257_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8814634.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="IV257_001517"
     CDS             12646..14664
                     /locus_tag="IV257_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8814635.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15091..17505
                     /locus_tag="IV257_001518"
     CDS             15091..17505
                     /locus_tag="IV257_001518"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8814636.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="IV257_001519"
     CDS             17853..19406
                     /locus_tag="IV257_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8814637.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="IV257_001520"
     CDS             complement(19787..21571)
                     /locus_tag="IV257_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814638.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="IV257_001521"
     CDS             complement(21586..23307)
                     /locus_tag="IV257_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814639.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="IV257_001522"
     CDS             23407..24168
                     /locus_tag="IV257_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8814640.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            25135..25403
                     /locus_tag="IV257_001523"
                     /pseudo
     CDS             25135..25403
                     /locus_tag="IV257_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            26075..>28349
                     /locus_tag="IV257_001524"
     CDS             26075..>28349
                     /locus_tag="IV257_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814641.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGL"
BASE COUNT         8941 a         5063 c         6001 g         8344 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaattccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagtgaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcagcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    24721 aaggttattg ttatttttat atattctttt taagaatgat tgttttttta tagaaatttt
    24781 aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata ttatagaagt
    24841 tgactggtag tgatttattt ttcgtcgttt tataaaaatg ttattttttg gaaaaattca
    24901 caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta tgactaatat
    24961 aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg gaatcaatga
    25021 aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat agcaatacat
    25081 cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac aacaaaagga
    25141 ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt tattctatac
    25201 aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa tatatggtac
    25261 agaatataga aattattgac attacagaaa atgatgaaga tttaatacag attgtcacta
    25321 tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg attaaggagt
    25381 tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga gcaaaaagag
    25441 taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt ttattagtaa
    25501 tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg tttagttgaa
    25561 ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc caagtattct
    25621 aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt gatgaaggtt
    25681 aaccaataca gggactcact gatgaaattc tagagtctga ccttggtatc aacgatttag
    25741 tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa taactattag
    25801 ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca taattatggt
    25861 taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac agaagacaat
    25921 cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa ccgaaaatac
    25981 gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta tcgggtacct
    26041 tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc ataaactgtt
    26101 aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa cggttctagc
    26161 tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc aagaggaggc
    26221 tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc taaatgcatt
    26281 ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca atgattacga
    26341 acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg caagaagcgg
    26401 tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa gcacaagtga
    26461 gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag aagaaagctt
    26521 aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg aatctcagat
    26581 tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc gccccacttt
    26641 gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg gcgatattct
    26701 tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct taattgagtt
    26761 cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt accaagaagg
    26821 cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag ccgctcagtc
    26881 gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa
    26941 taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg aattttcaat
    27001 tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct ctagagaaat
    27061 cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga ccaacagtaa
    27121 taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag tggttaccga
    27181 tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt ccactggaaa
    27241 tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag gtgaagcaaa
    27301 cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta ataaggaaac
    27361 gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt ttattgaata
    27421 tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg atgggttttc
    27481 tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact accgcttgag
    27541 aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca aaaatgttat
    27601 caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt ctggaacaag
    27661 aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt catttctatc
    27721 agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt tcagtgcgaa
    27781 taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg tagaggcgta
    27841 tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca atcgattgaa
    27901 tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta aaactgatga
    27961 gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat
    28021 acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc ctgtaggtag
    28081 ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga cggtacatga
    28141 tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa acatccttga
    28201 tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc ttcaaggagc
    28261 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggtttaa ctacaggaga
    28321 agatggaaaa ctggcaattg acggtttag
//
LOCUS       DACDIP010000030        28129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDIP010000030 DACDIP010000000
VERSION     DACDIP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.030"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(267..1166)
                     /locus_tag="IV257_001525"
     CDS             complement(267..1166)
                     /locus_tag="IV257_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8814642.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1176..1532)
                     /locus_tag="IV257_001526"
     CDS             complement(1176..1532)
                     /locus_tag="IV257_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8814643.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1887..2183
                     /locus_tag="IV257_001527"
     CDS             1887..2183
                     /locus_tag="IV257_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8814644.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2827..3780)
                     /locus_tag="IV257_001528"
     CDS             complement(2827..3780)
                     /locus_tag="IV257_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8814645.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3803..4558)
                     /locus_tag="IV257_001529"
     CDS             complement(3803..4558)
                     /locus_tag="IV257_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8814646.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4555..5514)
                     /locus_tag="IV257_001530"
     CDS             complement(4555..5514)
                     /locus_tag="IV257_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8814647.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5507..6457)
                     /locus_tag="IV257_001531"
     CDS             complement(5507..6457)
                     /locus_tag="IV257_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8814648.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6607..7059
                     /locus_tag="IV257_001532"
     CDS             6607..7059
                     /locus_tag="IV257_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8814649.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7148..9415)
                     /gene="gshAB"
                     /locus_tag="IV257_001533"
     CDS             complement(7148..9415)
                     /gene="gshAB"
                     /locus_tag="IV257_001533"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8814650.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9602..10600)
                     /gene="ftsY"
                     /locus_tag="IV257_001534"
     CDS             complement(9602..10600)
                     /gene="ftsY"
                     /locus_tag="IV257_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8814651.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10611..11420)
                     /locus_tag="IV257_001535"
     CDS             complement(10611..11420)
                     /locus_tag="IV257_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8814652.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11429..15010)
                     /gene="smc"
                     /locus_tag="IV257_001536"
     CDS             complement(11429..15010)
                     /gene="smc"
                     /locus_tag="IV257_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8814653.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(15030..15716)
                     /locus_tag="IV257_001537"
     CDS             complement(15030..15716)
                     /locus_tag="IV257_001537"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8814654.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16064..17845)
                     /locus_tag="IV257_001538"
     CDS             complement(16064..17845)
                     /locus_tag="IV257_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8814655.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17871..18785)
                     /locus_tag="IV257_001539"
     CDS             complement(17871..18785)
                     /locus_tag="IV257_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814656.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18812..19774)
                     /locus_tag="IV257_001540"
     CDS             complement(18812..19774)
                     /locus_tag="IV257_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814657.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19774..20718)
                     /locus_tag="IV257_001541"
     CDS             complement(19774..20718)
                     /locus_tag="IV257_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814658.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20719..21723)
                     /locus_tag="IV257_001542"
     CDS             complement(20719..21723)
                     /locus_tag="IV257_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814659.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21991..22233)
                     /gene="acpP"
                     /locus_tag="IV257_001543"
     CDS             complement(21991..22233)
                     /gene="acpP"
                     /locus_tag="IV257_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8814660.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22355..23356)
                     /gene="plsX"
                     /locus_tag="IV257_001544"
     CDS             complement(22355..23356)
                     /gene="plsX"
                     /locus_tag="IV257_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8814661.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23405..25441)
                     /gene="recG"
                     /locus_tag="IV257_001545"
     CDS             complement(23405..25441)
                     /gene="recG"
                     /locus_tag="IV257_001545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8814662.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25558..27240)
                     /locus_tag="IV257_001546"
     CDS             complement(25558..27240)
                     /locus_tag="IV257_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8814663.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27292..27654)
                     /locus_tag="IV257_001547"
     CDS             complement(27292..27654)
                     /locus_tag="IV257_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8814664.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27924..28112
                     /locus_tag="IV257_001548"
     CDS             27924..28112
                     /locus_tag="IV257_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8814665.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7790 a         6088 c         4945 g         9306 t
ORIGIN      
        1 actctcgact tgactgccta gacttcaatc aatattaatt ataattaaat aaaaaatgta
       61 tattatattt tttttgtgat tatcgttcaa aaaaagaata cacgcttgca tctagtactc
      121 tgcgagttac aaatcatccg atcaaataaa cattcgctga ttattttccg caaaaaaagc
      181 agcatatcca cgataaatgg atctactgct cttacttatc cttatgtttt acaaggtatt
      241 ttcttttaca cattactaat gctgtctcaa tacggaacga ttgtcatcgc ttgttcaaat
      301 tcctcttgca aatgttgtgt ttgataggcc tgaaaagctg ggtgatgggc acgcaagagg
      361 acacgtaatt tcataggatc atagctgtct agcaactgtt ctttcaagac aaaatctcgg
      421 gtcagctgcc attcataata ataaccaaaa ggtctgcagc tgtaaatcgt gtgaccgatg
      481 gatttatctt caaagcgacg atgcgtatgg ccaaaaacta cttgtttgat atttgaataa
      541 cggtcaagta attctccgaa tgattctgat cctaaaaatg catttaattt gttccaacgc
      601 tcataaggag cagattgata catgataaat gcccgtttag gaacgaaatg agtggataaa
      661 atcacctcaa gtttttttgc ttccagctgg tctagcaaat tttttgcagc tgcaagcatt
      721 tctgcattga cctcgagatc tgttttccca cgctggatga accgatcata ccaatacaaa
      781 cgcttcattc gtacgattgc ctcgtaatct ttcaattcgg aaaactgata gtcgtaccag
      841 ccattcatcc ctaaaagaac ggtctgctca tttaaagcaa tataccgttc atttaaaaaa
      901 tgtgggtctg gaaaatggtc catcatctgt tcattgggca catctgccaa ctcgtgattg
      961 ccgaaatgaa atgtcgtaga taatccttta ttgacgaaaa attcgacaac agctaggcaa
     1021 cggtctactt tattagccgt atctccggct agatgaagcc gagtgatctt gtgcttttgc
     1081 aaaacttgcc aaagctgcat cagctctgat tcgccgaatt tattgatgtc cacatgtagg
     1141 tcgctgatga tcgccaattt tcccatttta tccctctagt cttttcgaac aacttgcaag
     1201 ttgccttttc tcaagccgct tggtcctttt aattgtaaac tattataagt tggttctaaa
     1261 gcaagtgtgg atggatcatc catgtaaagt attgcactgt ctttgatttt gattggtcca
     1321 actggtgtat cagccactac agaataaaga cttagatctg tttgtgtatc ttttttgaca
     1381 atgatcaatc gaaatgccgt atccaattgg caactgactt ggctttctgc aaatggaccg
     1441 tcaccgtttt cgatatctaa taaaagttga tcgtctgctt ctaatttatt cttcaatact
     1501 tcttgtgctt cttttgtcac ttctaatttc atctgtatca cctcatgtca tagaataatc
     1561 actttattca tcagatgcaa ctaatttgtc tggttctgac tgttctcaat ttattggaaa
     1621 gtaagtcatt cgtttattat gatttctctt atcacaaaag agacaacttt cccttttctt
     1681 cgttcagttt cttgaacgga aaaagaccca aaaagcaacc tgttatttgt ttaaccttat
     1741 tcctttctta aataacaaaa aatactataa aaaataaaaa acctttaaca actattggaa
     1801 aaagaattac tttttgttca cggattatta acaactcttc ggtagagtaa acttatcaaa
     1861 taaaagactt ctttggagga atcactatga aggaatatat aaaaaacatc tattttatcg
     1921 aagaaacaca aaatatcgag ggttcttata ttgaagtaaa aacactcttt gtcaatgaag
     1981 ataaaaccaa agcattggat atttataaaa aattagcttc aaaaaaaacg aatagttttg
     2041 gattgatatt aagcgagtat aaaatcaaag cagaagagag ttatttctat caattactaa
     2101 aacggtggag taagcttccc gccgattttt atcgaaaaat gcagatcatc aactatcaac
     2161 cgcttgctga gacacatgcc taaatagagg ttgtgaaatc agtgctttga gctaataccg
     2221 aaggtcagct gattgaacat tgtttagaag agattgtgac aactgcgatt tgctttgagt
     2281 aataagaacg ctggacagaa aatagcttct catattttct agtctagtgg gcttattgcc
     2341 gagaagcagc agatggaaca tcgtttagaa gagattgtga caactgcgat ttgttttgag
     2401 taataagaac gctggacaga aaatagcttc tcatattttc tagtctagtg gacttattgc
     2461 cgagaagcag cagatggaac atcgtttaaa gaagagatcg gtacaaaata aaaatatcta
     2521 aatttaagtt ttatctaagt ttcgctttgt acctttgatt caaatcaaag gaggcgatct
     2581 tgatgctgaa aaaatggatc acactatttt ttgccactcc ccattctaga atcaacccgt
     2641 atgatgtaga taatgaattc acgttgaaaa aagatttctc aaataaataa aaaccatccg
     2701 taaaatcatc aatacgattt tacggatggt ttttgataac ttctttgagg ataggctctt
     2761 gctatttaag aatgtaagaa acattattgt tacatagtat gagagtttaa tcctctgtta
     2821 tttatgctat ttaaatgctt gattcacatc ttcgatcatc agtttcattg attctaaacc
     2881 gcccccgctt aaataccaga catctggttc aagggagatg atctgctgat ttttaccagc
     2941 attcgtttgt gctaccagtt cattggcaga aatatcatct ttgctgtcgt ctccaccaat
     3001 agcttttgtt ctgtctacta caaataagat atccggattt ttttccagca catattcata
     3061 agaaacactt tgaccgtgag tggatgcttc gatttgatca tctgcttgtt caaagccaaa
     3121 taaatcatgg atgaaaccga atctagaacc agatccgtag gctgataatt gcccttcatt
     3181 cacaagtgtg actagtgctt ttttgtcagt agcttctgct ttctttttcg tctgatcgat
     3241 tgcactagat aattcctcta attgagtttt agcttcttct tgcttaccaa agatctcccc
     3301 taaagcagtg acattttgct ggaaagattc ccatggattt ttcgcatcta aagaaaggaa
     3361 aatggtcgga gcaatttgac ttagatcttt ttgaaaatct ctttgtcggc cagaaatgat
     3421 gattagatct ggctgaagct gattaatttt ttctaagtct ggttctttta ttccacctgc
     3481 tgattctact tttttatagg atgaaagata ctcaggaagg ctgcttgtag ctgctccaac
     3541 taccttatct ccaacaccaa gagcatccat cgtatctaaa gaaccattgt cgaatacgac
     3601 gactttttct ggattatttg gcacctcgat ttggtctcca ttactatctg ttatcgtcaa
     3661 tgtagtttca gtttgtgtgc tgcttttttt tgtgtctgcc tgactggtgt tgttgctatt
     3721 tccacatgct gctaaaccaa atgatgctag aatcaagata cctactacta attttttcat
     3781 ctcttctctc ctaatgttag ttttagaaat aaagacagaa tctgcggcct tcaatctcac
     3841 aaatccgtat gtccatttcg tataattcat ccaatacatc tttttttatc atttcttggg
     3901 tcgttccttg cgtaaataac tgtccatctt tcattgcaac aatttcgtct gcataacttg
     3961 cagcaaagtt gatatcatga agaacaataa caattgtttt cccgatttct tgtactaaat
     4021 tttttaaaat ctgcatgatt tgaaccgcaa aattcatatc tagattgttc aaaggttcat
     4081 ccagtaaaat ataatccgtg tcttgcgcta gcaccattgc aatatacgca cgttggagtt
     4141 gaccgcctga aagagtgtca atcgattgat ccgctatttc ttctaaccca atgtgttcca
     4201 gtgattcctg aacgatttcc tcgtcttttg tcgtcaattt tcctttcgta taaggaaatc
     4261 gtccaaattc tactaattct ttcacggtga tttggacatt tatttcattc gattgtcgta
     4321 ggatcgataa ttttttggct aattcattgg atttccaagc ctttacttcg ttatggtcta
     4381 aatagatttc acctgtatct tttggtatca aacgactgat catcgacaac accgttgact
     4441 ttccagcacc atttggaccg ataaaagcag tcaacttctt ttcaggaatc gttaactgta
     4501 catctttaac cactgatttt ccatggtaca gtttattgac cttttgtaaa tcaatcatag
     4561 gatactcttc ctttcgcgaa tcaattttgt gacaaaataa attcctccga caaattcaac
     4621 gacgacactc actgttgtat tccagccgaa aatctgctct actgcgagct gtcctcctgt
     4681 cagaaacagc atagctacca gaaagcttcc gataaatagc tgaccatgac gataagtaga
     4741 aaagacacga taactgatat ttgcgacaat aaaccctaaa aaagtaactg gacccacaag
     4801 agccgttgat gtccctgtca aaagactgac acaacataaa atcaaaaatt caaaacgatc
     4861 tgtgtctatc ccgaggttta ctgcttgttc atttccgaga tggaaaacgt ctagcacatg
     4921 tcgatatcga aataacaggt accagcagcc gataatgatc aaagaagcag gcaaaagata
     4981 ctgactctgg atattgccaa agcttgcaaa taatctgcct tgaagcaaat catattcatt
     5041 cggatccatg atgacttgta aaaaggtact gacactacca aaaagcgtac ctaaaatcat
     5101 ccctgacatc agtaataaaa aaagattcgc tctcattttt ttcaaaagaa agaaaaataa
     5161 caatagactc agcccgacca tcagcccaac gttcaataag aaaaaaactg tacctgtctg
     5221 ctgaagcatc gtcactcctc cgataaagaa gaaaaggacc gtttggacaa ggacatataa
     5281 cgactcaagt cccaatactg aaggagtcaa aaactggtta tgcgtaattg tctgaaagct
     5341 gattgtggca ctgctgacag ctagcgagac aaggataaat gcaaaaattt tcttactcct
     5401 caatgcacat gcaaaatgcc agtttccata tgtatggatc gtcaagtaca ttcctgtaac
     5461 aaataaaaca agaacaagca taccaataaa aatagatggc ttttttttaa gcatatcgtc
     5521 gtccccttat cagaagataa aggaagatag ccccaccgat gattccaacg atcacactga
     5581 ctggtatttc ataaggaaaa atcaacacgc gagcgacaag atcgcaaaat actaagaaca
     5641 aactacctgc taaagcagtt acgccaattg ttttgtgcac ttgatctcca tatattctag
     5701 agacaagatt cggaatgatc actccgataa aaggaatgct tcctaccatg atcaacatga
     5761 ctgcactagc caatgatact aagccaatgc ctaaaaactg catcttctgg taatctattc
     5821 ccagatttac tgcaaacgat tcgcccagac caatgatcgt aaatcggtaa gcaaatagcc
     5881 aaaccaagag caacaatgga acaacagcat acaataactc ataatttcct cgtatcacag
     5941 tcgcaaaatt cccttgaagc catgaagtca tattttgtac caactggaat tgataagcaa
     6001 aaaaagtagc aactgaacca atgatattcc ctaacatgac tccaatcaac ggaagcatca
     6061 attgattttt ctggaggaac aatcgcgtaa gggataaaaa aagcaatgtt cctgcaaaag
     6121 caaagcaaaa tgctgtcaag gatcggacaa tcaaggatgc accaggtagg aaaatcatag
     6181 ccactaaaat acctaaacgc gcactatcca tcgtgcccgc agtcgttggt gaaacaaatt
     6241 tattttgggt caaatgttgc atgatcaacc cacaaatact actggtcgct cccacaagga
     6301 tcaaacaaat cgttcgtggc aaacgagtca ctagcaagat ttgtaagctt tgcccatcta
     6361 actggagcag ttctgacaat tgtacattcg atactccgat gaacaatgaa atactactta
     6421 aaataactaa taatgctact agtcgtccgt agcgcatcca atccaccttt ctaattgaga
     6481 atgattttca ttcttatttt aagtgaatcg ttgaacaaaa gcaaccgctt tttcatatgt
     6541 tttaggtatt attgggaata tgctataatc gtaagaaaga caaagagaag aaggagggta
     6601 ttttttatgg cacaagatga tgtaaaaagc catttggata agggacttta tggtacacct
     6661 ttagtgaacc ctgaagaaca gcataaatat ttagggacat tcagggaacg ctgctatctt
     6721 agtatgactg ttgctgaaat gatccaacca aaaaacaaag aaaacttttt aaaagaactc
     6781 aaaaaacatc cagatgcttc catactcttg aatggggctc tacccctttc tgtccaaaac
     6841 caatatatcc agctagcaac gaaaaacaat agccgtttca ctgtagtcaa cgattacgtc
     6901 actgcaaatc ctgaggaatt cggattgctc ttaacagcaa aagaagcagt aaatgaacct
     6961 gtgattgata tcgaaaaaaa atacccagaa gaaaactctt cgccagctga aaaagaaaaa
     7021 ccaaaaaaga aaaaaagctt ctgggatcgt ttattttaaa acgaaaaaac acataaattt
     7081 ttacatgcaa aaaaagcttt tgatcagatt gacatcaaat aggacaatct gtcaaaagct
     7141 ttttctttta aaatacttca ggatataaca atttcaatac attcatcgtc aatcttctgc
     7201 cttttcctgc aaaaggataa acatgcatat gcatggctgg attgaaattt gcttcgatga
     7261 tcccatatgc ttttttatca gttgtaggat cgatttcttt atctggtata atcaaatcga
     7321 taccactaat cttagctcct agtgcttcta ccgcagaaac ggcgattttt ttgtaagctt
     7381 cagaaaattc atcagtcata tcgatcgaat caccacctgt actgatattt gagttctccc
     7441 ttagataaac aatttgattg gcttggggaa ctgattcgat cgtcaaccct tgttctttca
     7501 acatcaattg ttctaattca cctaattgga tcaattctaa aggtgcacga tgattcgttc
     7561 cacgcaaagg gtcactattt ttttcttcaa caagttcttt cactgttcgg atactgtcac
     7621 caataacatt agcagggact cgcaacatga tggcttggac ttctccgtcg atcacaaaga
     7681 aacgatattc cgttcccggc ataaattctt ctactaaaac cgaactgtct tcacgaaaag
     7741 caatggctaa acctgcttga taatcttcca gactagctcc ttccttgaaa atcgtgatac
     7801 ctaacccgta attggttgat ttaggtttga tcacaaatgc ctgctcggca aatctcggat
     7861 aagcttttac cgcttcttcc atggatgaaa attctgcgcc tcctggtacc cggaacccag
     7921 cttcttttaa gacttttttc gttactgttt tgttttccat gataagtggg acgatatagc
     7981 tgtccttact tgtcatgttt gcatttttca catattctac atggtcttgg aattgcagac
     8041 gcaaaaattg atccgactca tctaatactt tgacttggac acctttttgg atggcatcga
     8101 acataaatat ctgagtagaa agttccattt cgcgaaaacc ggccaactga tagggacgtt
     8161 catggctttc tttataatat tgatgtccca atgccacagc aacttcttgc tgactcgata
     8221 gctgtgcttc ttgatagagt ttcgctgcta acgtttgttc aggatgatcc atttgagtca
     8281 atgcttcctc tactaaataa agagaatcca accaatccaa ttcaaccagc atttgcttca
     8341 ttccttcaag aatttctcta ccttcttgat gaaattctgt ttgatctgat ggttgttcta
     8401 gtgctacttt gttatttcga gtcgtacctt ctgctaccca ttggtcacag tcttcttttt
     8461 cttctgtcca taacatgtac atcaggaata attgtaagaa ccgaacttgc tctggattga
     8521 tacccaatct tgcataagga ttcaaatcga tattccgcaa ttcaatataa cgaacacctg
     8581 ttgttgctaa atctgcgact ttctttccgc ctcggaaacg aagcggtgta taaaattctt
     8641 tctcctccga aagtttccct tcttcgatca ttcgctcgat atctgctaaa tactgtttca
     8701 tagaagcata ggaaactttc acatttggat gattcgtata gcccagcgca ctgttgcgaa
     8761 tactgcgaac aggttcttgc ggatgtgatt catctaaaaa gtagttcttc tcactcattg
     8821 gtgaagcacc gaataaataa gtgatcatcc agcgatagcg cataaagttt ctcgctgttt
     8881 tcaaataaag ttctgttttg aattcggaaa aatcttgaaa ttcttcctga tggctgaaca
     8941 atgttcttag caactcatcg ccaaattcaa aattgaaatg aataccgctt accatctgtt
     9001 ttcgcttccc gtattctttt gctaaataac gtcgatacaa gacatcttcg aaatttttta
     9061 attttgcaat aatgatctct tcgtcttttt ccggtaaggc aggtggcata cttaatggcc
     9121 agatcatctc ttctttcggt atagaacgag cagtcacatc ataaacagca gcgagatact
     9181 gaaataattc gggaatagaa tcggtaacag gggtgatcat ctctgtttgt gtttcgctaa
     9241 aatctgtttg gatatagggg tgataggatc gatcgccaaa gattgctgga tgatcggttt
     9301 ttgctagatt tcctgcaaga tcaacccgct gtccttcccg ttctatacca aatcgagctt
     9361 ggtcgataaa agggcgcgca ttgacatgca ataataattg cttaaaattc atcattaagt
     9421 gacctccaat tcatatgaat ctaattataa tcaaaaagtc taagaaaaag aacgaaattg
     9481 tttgagaacc gcttacaaat aagtataaaa atgaaggcat caaaaacaca gctatgttaa
     9541 aaacaatcat tcaaacaagg agctgcaact tttgtcacag ctcctgcttt ttagacatgg
     9601 gttattcttc ttttagtaat cctttgaaca atccaacgac aaaatcattt ggatcaaacg
     9661 gctccaagtc atcgattcct tcaccaagac cgaccaattt cactggtaaa tgcaattcat
     9721 tacggatagc aagtacgata ccgccctttg ctgtaccatc taattttgtc aaaacaagac
     9781 cggtcacatc tgttgtttct ttgaattgtt ttgcttgaac catcgcgttt tgaccagtgg
     9841 ttgcatctaa tacaagaagt gtttcttggg gtgcttctgg taattcccgc tggattactc
     9901 gttttatttt ttctaattcg ttcatcaagt tcactttatt ttgcagtcgg cctgcagtat
     9961 caactagtaa aacatctgcc tgctctgtct ttgctctttc caaagcatcg aatacgacag
    10021 ctgctggatc gcctcctgca ttgccacgaa caacttcaac accagcgcgt tcgccccaga
    10081 caactaactg gtcgattgct ccagcgcgga aggtatctgc tgctgccatc aatacttttt
    10141 taccttcgtt tttgaactga tgggcaagtt taccaatact tgttgttttc cctacaccat
    10201 taacaccgac aaacaaaata actgttagtc cgtcttcttg gatatttagt tcatttactt
    10261 cattgattcc ttccgcttca tagagatcca caagtttctc gatgatcgca ttttggactt
    10321 ctgccgattt tttcgcattt ctgagtttca cttcttgacg taatgcttcg gtgattcgta
    10381 tcgctgtttc aaaacctaca tctgcaccga taagtgtttc ttctaattct tcaaaaaagt
    10441 cttcatcgac tgttcgaaaa ttggcaaaca actcattcat ccgttcacca aatgttttac
    10501 gcgtcttttc taaacccttg tcgtacttct cctgtacttc aacttcttct gttttctctt
    10561 cgccagtaaa agcacgcttg attttatcaa aaaatcccat ttgactcccc ctactctaat
    10621 acaaattttt ggatcacttt ggctacgcca ttttcttcat tgctggctgt cacgaaatct
    10681 gctgcttctt tgacgaaagg cacagcattc tccatcgcta cgcctagtcc agcatattgg
    10741 atcatcggta aatcattttc ctcatcaccg atcgccatga tttcttcttg tttcaaacca
    10801 agatcttttg ccaataaaga aataccataa gcttttgtaa caccttttgg cataaattcc
    10861 aaaagcatac ttcttgtttt gatgatctca tacatttcat ggaattctgc tggtatttct
    10921 ttgattttag gatctaattc ttcttggtca taagcgatta ctgccttatt aagcacaaat
    10981 tcatctgtaa tatcgcttat atccgctggt tggaattgca gcagcttatt caacacattg
    11041 tacaaggatt gcttccttgg ggcactaggc agctgtaaaa ccacattgtc agacaaaaca
    11101 tctagtggca gatccaattg ctgagccagt ttgtataagc gttggatatc tgatacttcc
    11161 atcactttct tttccatgat ttcaccagta tcattttttt gtaccaaacc gccgttgaaa
    11221 gtgatgctgt agtcaccttc atctaccagt cctaattctt gcagataatg cgccatcgca
    11281 gctaaggggc gtcctgtaca tagaacgatc ttgacccctt gctgttttgc ttgtgctaat
    11341 gcttgtttat tctcatctga aatcagcttt tgttcattta ataatgtacc gtccaaatcg
    11401 attgctacta atttgatcat tttcgttcct acactttcgt ttcgttgatt ttcgcctctt
    11461 cattgatatc ttccatccgt acagaaacga tttttgatac accggattcc tgcatcgtca
    11521 ctccgtacag tacatctgct gcgaccatcg ttcctttgcg gtgtgtgact acgataaatt
    11581 gtgtgtcatc ttggaaatcg ctcaagtaac gaccaaaacg tttcacgttg gcttcatcta
    11641 acgctgcttc tacctcatct aagatacaaa acggaacagg acagacacgg ataatggaaa
    11701 agagcaacgc gatggctgta agtgctcgtt ctcctccaga aagcaaactt agactttgca
    11761 gttttttgcc aggaggttgt acttcaatct caatacctgt tttcagcaaa tcagttggtt
    11821 ccgtcagtac taattcagct ttaccgccgc caaacatatt tggaaagaca attttgaatt
    11881 cttgacggat agcttcaaat acttctttga atcttgtccg cacttcatca tccatctcat
    11941 ccatcgtttc aaacaattgg tttttcgctg ctaacagatc atctcgctgt gttgctaaga
    12001 atgtatgtct ttcactgact tgttcgtatt gttcgattga atttaagttt actggtccca
    12061 gtttttcaat ctgttctttt aaggatgaaa cctttgttct actgtcttcg atatcggttg
    12121 tttcttgata gtcttgggaa gctttctcaa atgtcagctg atattctgac tgcagatagg
    12181 tcaaatgatt atctaaaatc atttcagctc ggtctttttg gacttctaat ttggattgtt
    12241 ccgacagtcg tgctttttgc tcacgattac gctctgccaa gactgcttca agcttatcga
    12301 tctccttttg ccttctatct cgctgctctt tggcttttgc caactcggct tttaatgttt
    12361 ctcgctgagc agataattcg ttaatttgtt tttccaaact ttcttctgtg acttcatgat
    12421 cagaaaagtc tgctgtcaat gtcgccaact gcttttctat cgcttcttga cgttcggttg
    12481 cctctgcttt ttggactcgg gcaccgcgga gctggatctg cagatgatta aactgctctt
    12541 tcaagacagc aagatctgct tgttcttgtg ctttttgact ttgtacttgt gctcttctgg
    12601 cttccatctg gtcgctttct tgacttaacg atttgatttc ttcgtcgatt ttttgtcgtt
    12661 gcgattcaag atctgtttgt ttgtctttca attcgttcgt ttgcttttcg taatcttcga
    12721 taaactgctg cagttcacgt gtctcaaaag ttgagatttg cttttctttt tcaaaacgtt
    12781 caagttcatt tatgatgttt tgtagctgat ttgtcgcttc ttgttcttca aaacgtaatt
    12841 gttcgcctcg tgtacgcaaa acttcttgtt cttcggctaa acgagctgtt tcttgttgca
    12901 gctcctgaac ttttttttct tgtgcttgga gttgtttatc tgcttcttca aattcactag
    12961 tcaactgttt caattcttga ttttgtacaa acaaactgcc ttggtttcct cgtttattcg
    13021 ctccacctgt catggatcct ccggcattca tcacatcacc ttctaaggaa accactcgat
    13081 attgatagcg gatcgtttgc gcgattgcat tggcactagt taagtctttg gctaaaagga
    13141 tcgttcctag caggttttga accacagttt ggatttgatc gggataactg acttgttcac
    13201 tggcaatccc tataaaccct tctacagctg ctgcttgtgt caatatatga gccggtaatt
    13261 gtcttggttt gattgtcgtc agtggaagga atgttgctct tcctcctctt tgctgtttca
    13321 aatacgtgat ggcttgccga gcatcttttt cattttcaac gatgacatgc tgggctgccc
    13381 cacctaaagc tgcttcgata gctaacgtga aatctgcagg aacatcgatc agttcagcca
    13441 ctgctccgac aatacccgaa agctgctgtt tatgctgcaa gaccaatcga accccttggt
    13501 aaaaaccaaa atagttttct tggatttctt gaaggctttt cttacgagca cgtagctgct
    13561 ggacttgatt cattaattga tacattttag gctgttcttt tgctaatgct tcttggaact
    13621 ttttcccgtt ttgttggatc atttctaact tctcttttgt ttcagttaaa gatgatttta
    13681 gctgcgcctg cttttgtgtc aattcttctt tttgtaagat caatcggtct actgatgctt
    13741 ctacttccga ttgcttggct aacgtctgct tgctttttgc tgtttcttgt atatattgac
    13801 gttctagata cttcagttcg tttcctacag ctgcttgttc ttgcatcaaa tcaacatatt
    13861 gatctcttaa ttctgccaat aattctttcg ttgatttttg gtatttttct aattcttgct
    13921 gtgtttttat gagatttgct tcagcttttt ggatctctgt ttctttttct gctgctgctt
    13981 tcatcaagct ttcttgaagc ttttcaaaat gtttaacctt tttttgtgct tctgctaatg
    14041 aagtttgata ttcttgcgag cttttttgtg tatgctttgt ccgctcctgc aaaacatctt
    14101 tttgtccttc tgtctgcttc agtttttccg aaagatctaa taatacttgc tgattttttt
    14161 cgatcagacg atcggcttgc gcattttctt tacgctgctt cgccaaaatg gattcttgtt
    14221 cttggatcga ctcagatagt ttgcctaatt ctagattgaa tttcgcaagc tgtgcttgtt
    14281 tgttgtccca atcttttttt gctgtcttga tttcagcaac catcaaagag acatcggttt
    14341 gagtcagtgt ttcttttagc cgtaaaaact cctttgctgc ctcactctgg gcggccaaag
    14401 gagttaactg ttcttccaat tcgtggatga tgtcttggac gcggctgagg ttgtcttctg
    14461 tttcaaaaag cttctgttct gctttctttt tccgctgctt gtatttcaag acaccagctg
    14521 cttcttcaaa gatcccacga cggtcttccg gtttactact gaaaatcgct tctacttttc
    14581 cttgagaaat gattgagaac gattcttttc ctaatccaga atctaagaac aattcttgga
    14641 tatcttttaa tctgcagctt tgtttattga tgaaaaactc gctttcccca gtccgtcgat
    14701 aacggcgggt tacactgatc tcattgtact ccaatggcag ataatgatca ctattgtcta
    14761 aaatcactgt gacttctgca atattcagct gcttacgggt atcggaaccg gcaaagatga
    14821 tatccggcat ttttccgcct cgcagacttt tagcagattg ttctcctaat acccagcgga
    14881 tagcctctgt aatattgctt tttccgctgc catttggtcc aaccactgct gtgacgctat
    14941 tttcaaaatc aatgactgtc ttgtctgcaa atgatttaaa accggctatt tcaattcgtt
    15001 tcaaatacaa agctttccct cacttacttt tacagtgctg ccaatgcatt ttccgccgca
    15061 gcttgttctg ctaatttttt cgatttccct tgtccagtgc caatcaattg atcatctaca
    15121 taaacttcga tccaaaatac tcgttcatga gctggacctt cttcattgat caatcggtat
    15181 tcgattgata catcacctga tttttgcaag acttcttgta gttttgtttt atgatccatc
    15241 tcatgtgaaa aagcacctgc tttgacttta ggaaaaatca ctttttcgat gaatgcatgg
    15301 gtcgtgtcga atccctggtc aagatataac gcgcctaaaa aagcttcgaa taggtcgcaa
    15361 agcaatgctg gtcgcgtacg tcctcctgaa ttctcttctc ccttgcccaa caaaacatat
    15421 tgatcaaagt gacattcttt ggcaaatttc gacaagctgt cttcgcgaac gatcgcagct
    15481 cgtgtttttg tcaattttcc ttctggcaaa tgaggatata aacggaataa atactgagaa
    15541 accatcagtt ctaaaacggc atctcctaaa aattcaagtc gctcattatc cgataattgc
    15601 agattgcgat gctcattcac ataggatgaa tgggtaaaag cttgttcaag caaatttaga
    15661 tcgtgaaaaa cgatgttata gttttctttt agttcttttg tcagctggtt gtccattttc
    15721 cacattcctt ttatggatat tttttcgata cttccttatt atactggttt tttgtcaaat
    15781 ttgaaatggt tcggtcaaaa ggtaagtaaa gctttaataa tcctagtgat tgacgagaag
    15841 ttgtgaaaaa ttcgtttagt ttcgagtatg aagaaaaaat tttgtgaaaa tggctttcca
    15901 tatttttgca taaatttgac ttaataccga gaaactggac tttgaacacc gtttattgga
    15961 gaagtcatga aatcagcgcc ttgacctgaa tattagagaa ataaacagaa aaatatcaaa
    16021 aaaccgaaaa aggacatcgc cctttttcgg tcatttttag tgattatgaa cgtgaatctg
    16081 ctgtaacaga aacatttgcc catgggtttg gtacatcata agaccaatcc cagtctttga
    16141 cacggtcgtt gactggcaat acttcattac ggaacaacgt tggaatcgca aatgcttctt
    16201 caaatgcata ttgttgccat ttttgatatg cttctttttg gtactcttca tcaaatgctt
    16261 tgcttgaact gatatcgttg atcaattttg tattttcttc tgattcataa cgagtgtagt
    16321 tgaacgcagc atttggtccc catagtccac ttggatttgg atcagttcct gtattccaag
    16381 cagcttggta aacatcaatt tcaggatcat cgttttctaa tttatcatag aaagaattga
    16441 attcgatcag acgacctgtt gtgtattgga catccaaacc aatttctttc cattgttgga
    16501 tatagtaatc tgccaatggt tgcgctgttt ccccaccgga catcgaagca aagttgattg
    16561 ttagtttatt tccatctttg tcttctctta aaccatcgtc atccacatct ttgtagccag
    16621 catcatctaa tagttttttc gctttgtcta gatcttgtgt gaatcctttc atctcatcat
    16681 catggtatga ctggaatact ggaggaatca atgaagtcgc attgcttctc aaacctgcat
    16741 agaaacgttc acctactgca ttattatcta atgcatagcc cattgcttga cgtaatgatt
    16801 tatcagccat ttttgaatct ggattgtatt ccacactctt tgtatctgca ttccatttac
    16861 ctaacttgaa tcctagataa gtataagaag tttgttcacg gcctaagttt gtatagccag
    16921 caatttcttt ataggtatcg taagtatctg ttggcatttg gaaaatcata tcatataatt
    16981 tagagttcaa tgcttcagtt gcacttgcca tcggtacaga tttcatcgtg atctttttca
    17041 atttaggcgt accattccag tagtgctcgt ttggcaagta ttctactgat tctcctgcaa
    17101 cgatattact catgtaataa gggccaaaag tcactggatt tttacgtact tgatcgcttg
    17161 attccatatc tttgattgct acaccttcta acgtatgttt tggtgctgca tatgaccaga
    17221 taccaccacc cgattgaagc atgcttgggt ttacttcttt atattcgatt tcaactgttt
    17281 tgtcgtctat ttttttgatt ccggagattg tgtcggcact tccatcatga tattcttcca
    17341 tacctacgat gttacggaaa gtgtcgtcat aacggatacc agtgtaatct ttattcccaa
    17401 tgatttcgta agggaaaatc acatcgtccg ccgttacatc ttcaccatct gaccatttga
    17461 cgttgtcttt tactttgatt gtcgcttttt tcgtatcttc atctagatca agcgttgcag
    17521 ctccgtcgtc tgtgattctg aaatcgtcat cgtttgcaaa tagcggttca tgagaaggag
    17581 ccataaaagc tgcatcatat gaatcttggt aaaactccca ctggaacaat cctttaaact
    17641 gactgtccgt tgcgaccgca gcttctaatt cgccgtcttc gattgcatca tcattatttt
    17701 ttgttgcaat tggaaaagtt aattcctctt tgttattgcc gttgcctcca gagttgtttc
    17761 cagaggtatc tgtacctccg cccccgcatg ctgcaagaac gacagcagac atcagcgctg
    17821 caaatccaaa aatcgttttc ttcattccta tttccccctt cgcttctata ctagcctaac
    17881 cgttggcgcg catctgcaga acgcttgaat gcttgtccaa cataattgat actcaacatc
    17941 aaaactaaaa taaggattga tgcgggtaac cagatccatt gccgattcac taagacttct
    18001 ccgttacttg cataaccgat caatgtacct aaactaggta catttgccgg caagccgaaa
    18061 cctaagaaag tcaaagttgt ctcgataccg atgtttgccg caaagttcat cgtcaaatta
    18121 gtaatgatta atgaacttag attcggcatg atttcgcgga acatgatttt aaaatcattg
    18181 gttcccattg tttttgaggc actgacataa tctcttcttg cttcagataa tgtcttgctt
    18241 cggaataatc gggctttagc tacccaataa aatgcactca tgatgaatat aaaggaccag
    18301 acattgtatc tggaaataat cgatacgaag acgataatga tcagcatgat cggtagaatc
    18361 atgataaaat caacgatccg catcaagatg ttgtcaatga tccctccgta gtagccagag
    18421 atgatcccta atccaacccc gatgatcgaa gtaaggatcg tgatcgcaaa tccgatcgta
    18481 acagagtttc tagcacctat gatcaattgt cctaatacat cacgtccgcc ttcatctgct
    18541 cccagaatga acttttgtcc ttgtgtcgaa actgttcctg gtgcagcgta tttgtcgaag
    18601 atactaacat acatcacttt gtcttgatcg gtcagcatcg caccgataaa gatcactaat
    18661 aataaaacca ctagcaaacc taatgaaaaa atagcaattt tatctttttt aaattctcga
    18721 gcaatcattc gtatccccat aggaggaata ctttcttgaa ctgctgtatt gtttttttca
    18781 ctcatcgatc cattctcctt tctgattggt tttattgaat ccgaatacgc gggtcaacga
    18841 tactcatgat gatatctgaa agtaatgtcc ccactagcgt cgcgattcct aaaatcaata
    18901 ctaaggatgt gatcacggta tagtcccgtt ggccgatgga atcaataaat agttttccga
    18961 ctcccggata accaaaaatc ttttcgataa ctacagaacc agcaatcaac gaagtgattt
    19021 cataccccat ttgagaagcc atcggcaacg ctgcatttcg gaaaatatgt cgactgaata
    19081 ctttattcgt cggtacccct ttggaacggg ctgttctgac aaaatcgagc gatttcgaat
    19141 cgattacttc acttcgcaga tattggattg tgacggcagt cgccaaaaat gcttgggtaa
    19201 ttgatggtaa cagaatatat ttcaaacggt ctatccattg agtgataaat ccaccttgca
    19261 aacctagtgt ttgtgatcca cttgttggga accagcctaa acgataacca aaaacaaata
    19321 gcatgatcaa gccgaagata aataaaggaa cagcaaaact aaagaagttg tagattacga
    19381 caatcttatc caagattgaa ttttgatacc ggccagcaag catccccaga ggcacagcaa
    19441 tcaaataagt aaggacaact gtcaagatcg atagatataa agtgttgatg atccgttcgc
    19501 cgatcaagtc agcaacagag cgtttgtata agaaactttg accaaaatcg ccttgcaaag
    19561 catttccgat ccaacggaag tactggacat accatgggtc attcaagcca gctgcttgtc
    19621 gcatctgttc gatcactgcc ggatcctgat ttggattgat caaaccggta aatggatccc
    19681 ccggcatcgc ttgggctaat aagaagatca gaacacttaa aataataact tgcgggatca
    19741 tcaataaaac acgtcgcaga atcgttttcc acattagctt gcacctccat ctttcaacgc
    19801 cactttgtgt gtagttgtga tcgtacgcaa atcgtagact cgaccagtcg catcatagta
    19861 ttctttttgg ttttggatat actcttgttc tactcttcgt cgattttctt tgtggctttc
    19921 cctattttct acatcgattt gcggaatagc tgaaagcaga cgtttcgtat agatatgtcg
    19981 cgggtcatta taaatgtctt ctcttgtacc gatttctaca aatcgacctt tgtacataat
    20041 tgcgatattg tcgcacatgt gttttactac acctaaatca tgtgaaatga ataaatagct
    20101 gaggccgtat tcttgctgga ttcgtttcat gaaattcaat acttgcgctt gaacagatag
    20161 atccagagca gatacaggtt catcggctac gatcagtttt ggattggtag caacagcacg
    20221 ggctactcct agacgttgac gttgtccgcc tgaaaattca tgaggatatt tatatagcgc
    20281 atcttccggc atcccaacga tatctagcag tccttttacc tttttctttt cttcttgatc
    20341 gcttaaccgt tcaaaatttc ggatgggttc agcaatgata tccaacactc gctttttagg
    20401 attcaaactg gacattgaat cttggaagat catctgcaca tccttgttat aacccatttt
    20461 tttgcggttg cttcgcttcg ttacatcttt tccttcatac atgatttgtc cggctgttac
    20521 cttttctaat cccacaacgg cttttcccgt agtagatttt ccagagccag actctcccac
    20581 taaaccatat gtctttcctt tttcgataat aaaatccaca ccatctacag catatacatg
    20641 atccgttact cgattgaaga atccactgcg aatagggtaa tgaactttca ggtctttgat
    20701 tgtgattaat tctgtcatct acacctctcc tttttcgtct ctaaagtgga aatgcttata
    20761 gcaggagcaa cgtaccaagt ggttcggtgc gacttcatga aggataggct cttcttcatg
    20821 atcgcttgct ggtatccatg gaatacgtgg tgcaaaccga caacctttac gaggcatgtt
    20881 tttcagagaa ggaacgacac cttcgatcac atggagttct gcatcatgat ttccttcttg
    20941 tggtatagag ttcagtaaag agcgtgtata aggatgctta ggatgatcaa acagttcttt
    21001 tacacctgct acttcaacga actgtccgcc atacatcact gccactcgat cagccatttc
    21061 tgctacgact cctaagtcat gagtgatcaa gatgatccct gtttttgttt cttcctgaag
    21121 gtctttcagt aaatcaagaa tctgtgcttg gatggttaca tctagggctg tcgtcggttc
    21181 atctgcaatg acgattggag gtttgcaggc gattgcgatt gcgatgatga cacgttgtct
    21241 cataccgcct gatagttcat ggggatattg gcgtccaact ctcgcaggat taggaatccc
    21301 tacttgttct agcaattcta gtgcacgagc ttgacgctgt tctgcattca tctttgtgtg
    21361 ataggaaagt ccttctttga tctggtcttc gattctcatg agaggattta atgcagacaa
    21421 cggatcttgg aaaatcattc cgatatcatt tccgcggatt ttattataga gcgtttcgtt
    21481 caggtcgatt aaattcagat cgttatataa aatctcacct gtaatttttg tgttattagg
    21541 atcaagtaat cccataatcg tagtagccaa ggtacttttc ccgcacccag attccccaac
    21601 gattgctaaa atctcgtttt tgtctaaagt aaatgaaacg tcatctactg catcgtaata
    21661 gtcatctttg agtcggaatc ctgtatgtag gttctgaact tctaataatt ggttatcgga
    21721 caatcgaata cccctctctg cttcctaaat ggaagttttt cttaataaaa aaggacatta
    21781 cacagaataa atcgttttat aacaaaggtt attcttatct ttgtgtaatg aattattaat
    21841 aattatatag aaaaccgctt tactttgcaa cataatttct gtttatcgcc agaattatca
    21901 gaaaaatgac ttttacgtgt caaaaagatt aaaaaaagaa ggataaaaag ctgcatgcag
    21961 ctttttatcc ttctttgctt ttatttgaaa ttatttaaga tggctgctga tataatctac
    22021 tgcgccaccg actgtttcaa tttgttcagc gtcttcatct gaaatttccg taccgaattc
    22081 atcttcaagc tctaaaacga attccataat actaattgaa tcagctttta aatcatcttt
    22141 gatgttcaat tcatccgtca ctttatcggt atctatctca aaatgatttg aaataatttt
    22201 cgctactttg ttaaatactt cttcgcgagt caactttttc acctccaaga atcatacact
    22261 tgtcaattac ctacaccatg gctaatttta ctggcatttt tcaggaatgt ccaccttttt
    22321 ttcaatggaa cggatcgctt cagatatcca aatactattc ttcgcttttt tcaaattgtt
    22381 cgactaattg tccaactaca tctgtctcaa gcattgtgtg gatttggcgg atcgtatagc
    22441 gaacagcatc ggggcctgtt gctccatgag ttttaatgac tggtgctttc aagccaaaca
    22501 aaacggcacc gccatgtttg gaataatcca tttctgcttt caaagaacgt aagccgtctt
    22561 ttaaaagcat agcgcccatt tttcctttga tcccttcatt tagaatagca gattttaata
    22621 gattcatcat attcattgct gttccttcaa tagatttcaa aacagcgttg cctgtaaatc
    22681 catctgtgac cactacatca gccacaccat tgagtagctc acgagcttct acgtttccaa
    22741 taaaattcaa gcttgtttca ttctgaagga gttcataggc ttttttcgtc acttcactac
    22801 cttttgttgc ttctgttccg ttgtttaaga gtgcgacacg aggacgtccg atacctcgga
    22861 ctttacgtgc ataaaaagaa cctaaaatac cgtattgcaa caaatgttct ggtttgtttt
    22921 ctgcgttagc gccaagatcc agcatatcga aaccggcacc ttctttccct actactggca
    22981 aggtagacat caagccaggg cgttcgatat ttttgatacg tcctacgatg aacaaccctg
    23041 ctgctagtag cgcgcctgta ttccctgctg aaaatacagc atctgcttca ccgtttttca
    23101 ctgcttgtgc agcaaggacc attgatgcag tcttttttcg tcgaattgct tttactggct
    23161 catcatcact attgattttt tcgtctgtat ggataatcgt gatgtttttt tcatttgtta
    23221 agtatttctt gatttctgct tcttttccgt aaagtaagaa ttctacttct ggaaaatctt
    23281 gctgcgctaa agccacgcct tctacaattg cttgaggtgc gtgatctccg cccattgcat
    23341 ctacggcaat tttcattgtt tccactcctt tttccgtcat cagtataaag tatagcacag
    23401 tctgctaatc gaaataacga ttatcctgat tttctatttg gaggctttgc gctaaaggct
    23461 gatactcagg aagcatctgc caatccttta tttgccaaag ggctgcagcc tcttctctgg
    23521 ctacctccaa aacatttcca tccgtcacca agtcagcaat cgcaaattga ggcagaccag
    23581 attgacggaa gccaaatact tcaccaggac cacgtaattc cagatctttt tcgcttagta
    23641 caaaaccatt ggtggtctct gtcatgattt tcatccgttc cacacctagt tcatttttag
    23701 gattagcaac taagatacag taggaagcat cacttccgcg tccaacacgt ccacgaagtt
    23761 ggtgcagctg tgccaatcca aatcgatccg catccatgat cagcatgacg gtcgcgttcg
    23821 gcacgtttac ccctacttca attacagtag tcgaaactaa tatctgcatc tggttgtctt
    23881 tgaaggcttc catgattact tctttttcct gattcttcat tttaccatgt aacaagccaa
    23941 cttcatattt tggtgcaaat agttcacgaa gtttttcata gatttccact gcatttttta
    24001 catctaatgc ttctgattct tcaatcagcg gacagatgac atacatttga tgtcctcttg
    24061 ccagttcttt tttcgtccaa tccagtacac tgtccaattg aggtgttctt acccagcgtg
    24121 tttcgatcgg gatacgtcca gctggaagtt catcaatgat agatacatcc atctcaccat
    24181 atgctgtaat agctaatgtt ctaggaatcg gagtcgctgt cataaataag acatctggtc
    24241 gccagccttt ttcacgaagt actttccgct ggttcacgcc aaaacggtgc tgctcatccg
    24301 taataactag acctaaattc tgaaattcga ctccatcttg gatcaatgca tgtgtgccga
    24361 tcacgacgtt aatttcccct tgctctagtt tttcaaggag ctcacgtcgt tcttttgctt
    24421 tcgttgaccc cgtaatcaat gcgatggtta cttcattggg atcaaacagc tgttgcaagc
    24481 tttccatatg ttgttgtgcc aagatttctg taggcaccat caacgcacct tggaaacctg
    24541 ctgtaacggt agcgtataac gcaatcgcgg caaccactgt ttttccgcta cctacatctc
    24601 cctgcaaaag tcgctgcata tgctgcggac tgcgaagatc ccgacagatt tcattcgtta
    24661 cacgtttttg tgcatttgtc agttcaaaag gtagcttttg agtaaattgt ttcaattttt
    24721 ggacatcata aaggatctcc aaaccattcg tttctgcttt ttctgctttt ttcaatcctt
    24781 gaagacgcat ttgaaataaa aagaactctt caaaaacgac acgtcgtttg gcttgatggt
    24841 tttcttcgtg gtcttttgga aaatgcatac ttcttacagc agaagctcgg tcaagcagtc
    24901 gatatttctc cacaaggttg tttggcagat tttcttcaat caatgtgccg tattcttgaa
    24961 accctttttt gattagatca actaatgtcg tttgacgaat tgatttgttg acgtgatata
    25021 tcggcgaaaa gtcatccatt gtttgagaac ctaaaatctt catcccgttc aaggctttac
    25081 gctttgcatc ccatttaccg tatacggcga tttcctccga taaaatgact ttttctttta
    25141 gataaggttg attaaaaaaa gaaacgttga acacatcatg ttcttgcatc atacgaaatt
    25201 gcaatcggct ttttttataa ccaaatcgat tcatcacagg aggcgagact acgattcctt
    25261 tcaaagtaac cttttcttga tcctggatct cgtttagatt tctttcctga atatcttcgt
    25321 agcgaaaagg ataataagtc agtaagtctt caatcgtttg gatgcccaag ctggcaagac
    25381 tagctgctct tttttctccg actcctgcca gcatagtgat tggatcatct aaagaacgca
    25441 taaatttttc ctccctcctt taacaaaaaa aggacttggt cagcgatcat acctgatcac
    25501 taaccgaagg ccttttatga atgaatactg tgttagaaag atattctgac acagaaatta
    25561 ttcagcagaa aatagataag gatagacagg ctgatctcct tggtggatct cgatttccaa
    25621 atcaggatgt ttttctgtta gtttctcagc aaattcattt gcttgttcaa ctgttccttc
    25681 atcacctgcc aagatcgtta cgatctctgt gtcctcattg atcatttgtt ctagtgtact
    25741 taacgctgtt gctgtgatat ctgggttaga gatcaggatt tttccatcga tcatgcccaa
    25801 aaagtctcct tctttgatct ctacaccgtc aattgaggta tctctgatcg cgtgagtgac
    25861 ttgaccgctc acaacgctag acaacatttc tgtcatcgtt tctttgtttt cttctaaaga
    25921 agcttgatca ttgaatgaaa gcatagctgt caatccttgg gaaatcgttc ttgaaggaac
    25981 aactgctact gggatatctg ctacttctgc tgcttgatca gctgccatga agatgttctt
    26041 attgtttggc aagacaatca ctttatctgc gtttacttct tgaatcgctt taacaatgtc
    26101 ttccgtactt ggattcattg tttgtccacc actgataaca taattagcgc ctaaactttc
    26161 aaacagctct tggacgcctt taccagcagc gatcgctacg ataccatacg ggatacgtgg
    26221 tatttctttt ttcgcaaact cgttcacttc ttcatcgtgt tccaaaatcg tctcatgctg
    26281 caagcgcatg ttgtcgactt tgattttgac taatgaaccg aatttttgtc cataattcaa
    26341 gacttctcct gggttttctg tatgcacgtg gactttgatg atctcgtcat cattgaccac
    26401 aagtaaagaa tctcctatac catctaaata gttacggaat tcttcgtaat cgaatgtact
    26461 atcgactgta ggtccttctc cgatttttac catgatttct gtacagtaac caaaatggat
    26521 atcttctgtc gccatttgac cttggacact gcgatgatgt tctgcattga ccatatcatc
    26581 catttctgct ggagaaggtt catatgtttc atccgcttgg aattctccat tcaatgcact
    26641 taaaaagccc tcataaacaa acagcagtcc ttgccctcca ctgtctacga caccgacttc
    26701 ttttaggact ggaagcaagt ctggagtttt agctaaagca cgctttgctc cttttacaac
    26761 agctgtcatt acttcaacga catcatcagt actttttgct ttcttttctc cagctttcgc
    26821 tgcttctcta gctactgtca aaatcgttcc ttctactggt ttcattactg ctttatacgc
    26881 agtttcaact ccatgagtga aggctgcagc aagatcagaa gcatttaatg agacaacatc
    26941 tggaatttgc ttagaaaatc cacgaaacaa ttgagacaaa ataacgccgg aatttccgcg
    27001 cgcacccatc agcaatcctt ttgaaagcat tgctgctaat tctcctactt tttctgaggt
    27061 tgaatctact actgccttag ctccactagt catagataga ttcatatttg ttcccgtatc
    27121 gccatctggc acagggaata catttaatga attaacgtat tctgcattta cttgcagacg
    27181 attcgcacct gcctggacca tttcctggaa ctggcttgca cttatttctg ttacactcac
    27241 ctagataatc ctccttcaga tcgactgctg agttaccagc cagtctcctg cctagtctgg
    27301 cagtacacga acaccttgta caaagacatt aactgaattg gcagttacgc ctaataatgc
    27361 ctctagattg tatttcactt tttcttggac gttgcgggaa acttcagaga ttttcgtacc
    27421 atagctgata atcgtataaa catctacagc aataccatta tcttcttggc ggacaactac
    27481 tcctcttgag taattttctt tacgcaaaat ctcagtaata ttatctttaa tttgattttt
    27541 gctggccatt cctacaacac cgaacacatc tgtcgcggcg ccaccaacaa ctgttgcaat
    27601 tacatcattg gtaatttcaa tggttccagc tgacgtttga atttttacag ccatgaatga
    27661 gcctccttaa cgggtaaaaa actcccattt ttcttctatc atattttatc atagcgcaac
    27721 ttgaaataaa aggcagttac ccaaagtact caaaaacctt gcaaaatttc aggtataacc
    27781 ttgtttaatt tacaattatt cttctcaatt gtcaattgta tctatgaaaa tgtacttgca
    27841 aattcccatt ctatatgata tgttagtctg gtatgagctg aattattcta ttgctccaaa
    27901 tcaaggcaaa ggaggaaatt accatggcaa aagtttgtta ctttacaggt cgcaagacaa
    27961 aaagtggaaa caaccgttca cacgctatga actcaacaaa acgtactgtt aaacctaact
    28021 tacaaaaagt tcgcgttatg gtcgacggaa aacctaaaaa agtttgggtg tcaactcgtg
    28081 ctttgaaatc aggaaaagtt gaacgcgttt aattgaatga tacttggac
//
LOCUS       DACDIP010000031        28115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDIP010000031 DACDIP010000000
VERSION     DACDIP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.031"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(548..910)
                     /locus_tag="IV257_001549"
     CDS             complement(548..910)
                     /locus_tag="IV257_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814666.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(984..1364)
                     /locus_tag="IV257_001550"
     CDS             complement(984..1364)
                     /locus_tag="IV257_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8814667.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1446..2621
                     /locus_tag="IV257_001551"
     CDS             1446..2621
                     /locus_tag="IV257_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8814668.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2698..3282)
                     /locus_tag="IV257_001552"
     CDS             complement(2698..3282)
                     /locus_tag="IV257_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8814669.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3483..4481
                     /locus_tag="IV257_001553"
     CDS             3483..4481
                     /locus_tag="IV257_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8814670.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4593..5102
                     /locus_tag="IV257_001554"
     CDS             4593..5102
                     /locus_tag="IV257_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8814671.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5132..5791)
                     /locus_tag="IV257_001555"
     CDS             complement(5132..5791)
                     /locus_tag="IV257_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8814672.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5793..6557)
                     /locus_tag="IV257_001556"
     CDS             complement(5793..6557)
                     /locus_tag="IV257_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8814673.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6565..7242)
                     /locus_tag="IV257_001557"
     CDS             complement(6565..7242)
                     /locus_tag="IV257_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8814674.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7279..8664)
                     /locus_tag="IV257_001558"
     CDS             complement(7279..8664)
                     /locus_tag="IV257_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8814675.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8874..8958)
                     /gene="ffs"
                     /locus_tag="IV257_001559"
     ncRNA           complement(8874..8958)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV257_001559"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8981..9496)
                     /gene="tadA"
                     /locus_tag="IV257_001560"
     CDS             complement(8981..9496)
                     /gene="tadA"
                     /locus_tag="IV257_001560"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8814676.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9603..10460
                     /locus_tag="IV257_001561"
     CDS             9603..10460
                     /locus_tag="IV257_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8814677.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10541..10876)
                     /locus_tag="IV257_001562"
     CDS             complement(10541..10876)
                     /locus_tag="IV257_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814678.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10943..12313)
                     /locus_tag="IV257_001563"
     CDS             complement(10943..12313)
                     /locus_tag="IV257_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8814679.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12391..12927)
                     /locus_tag="IV257_001564"
     CDS             complement(12391..12927)
                     /locus_tag="IV257_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8814680.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13236..14717
                     /locus_tag="IV257_001565"
     CDS             13236..14717
                     /locus_tag="IV257_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8814681.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14820..15527)
                     /locus_tag="IV257_001566"
     CDS             complement(14820..15527)
                     /locus_tag="IV257_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8814682.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15606..15944)
                     /locus_tag="IV257_001567"
     CDS             complement(15606..15944)
                     /locus_tag="IV257_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8814683.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(16055..17017)
                     /locus_tag="IV257_001568"
     CDS             complement(16055..17017)
                     /locus_tag="IV257_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8814684.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(17032..18363)
                     /locus_tag="IV257_001569"
     CDS             complement(17032..18363)
                     /locus_tag="IV257_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8814685.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18371..19051)
                     /locus_tag="IV257_001570"
     CDS             complement(18371..19051)
                     /locus_tag="IV257_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8814686.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19270..19749)
                     /locus_tag="IV257_001571"
     CDS             complement(19270..19749)
                     /locus_tag="IV257_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8814687.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19876..21555)
                     /locus_tag="IV257_001572"
     CDS             complement(19876..21555)
                     /locus_tag="IV257_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8814688.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            22103..23605
                     /locus_tag="IV257_001573"
     CDS             22103..23605
                     /locus_tag="IV257_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814689.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23618..24280
                     /locus_tag="IV257_001574"
     CDS             23618..24280
                     /locus_tag="IV257_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814690.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24381..24584)
                     /locus_tag="IV257_001575"
     CDS             complement(24381..24584)
                     /locus_tag="IV257_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8814691.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24695..25879)
                     /gene="iadA"
                     /locus_tag="IV257_001576"
     CDS             complement(24695..25879)
                     /gene="iadA"
                     /locus_tag="IV257_001576"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8814692.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(26036..27775)
                     /gene="yfcC"
                     /locus_tag="IV257_001577"
     CDS             complement(26036..27775)
                     /gene="yfcC"
                     /locus_tag="IV257_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8814693.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8356 a         5840 c         4729 g         9190 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcag
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg
      241 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
      301 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      361 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      421 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      481 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      541 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      601 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      661 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      721 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      781 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      841 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      901 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      961 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
     1021 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
     1081 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
     1141 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
     1201 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1261 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1321 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1381 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1441 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1501 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1561 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1621 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1681 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1741 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1801 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1861 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1921 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1981 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     2041 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     2101 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     2161 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     2221 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2281 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2341 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2401 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2461 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2521 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2581 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2641 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2701 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2761 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2821 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2881 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2941 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     3001 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     3061 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     3121 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     3181 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3241 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3301 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3361 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3421 aaacattttt gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt
     3481 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3541 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3601 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3661 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3721 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3781 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3841 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3901 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3961 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     4021 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     4081 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     4141 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     4201 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4261 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4321 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4381 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4441 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4501 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4561 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4621 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4681 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4741 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4801 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4861 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4921 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4981 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     5041 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     5101 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     5161 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     5221 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5281 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5341 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5401 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5461 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5521 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5581 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5641 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5701 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5761 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5821 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5881 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5941 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     6001 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     6061 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     6121 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     6181 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6241 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6301 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6361 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6421 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6481 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6541 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6601 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6661 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6721 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6781 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6841 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6901 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6961 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     7021 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     7081 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     7141 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     7201 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7261 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7321 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7381 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7441 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7501 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7561 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7621 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7681 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7741 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7801 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7861 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7921 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7981 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     8041 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     8101 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     8161 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     8221 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8281 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8341 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8401 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8461 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8521 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8581 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8641 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8701 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8761 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8821 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8881 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8941 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     9001 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     9061 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     9121 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     9181 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     9241 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     9301 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     9361 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     9421 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     9481 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     9541 tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca
     9601 agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag
     9661 catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag
     9721 attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc
     9781 aaaaaaaaca cccatggtat cactatcttt ttaaagatgc ttcttgtgtt caagaaaaaa
     9841 gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc
     9901 aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag
     9961 atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta
    10021 tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc
    10081 attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag
    10141 agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt
    10201 actatacaaa agatactgta aaagatagta agttgatcaa aagttacata gaactgatca
    10261 aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct
    10321 ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc
    10381 aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat
    10441 tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac
    10501 cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa
    10561 aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag
    10621 agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac
    10681 tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga
    10741 cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg
    10801 atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt
    10861 ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa
    10921 aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta
    10981 ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc
    11041 atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt
    11101 gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc
    11161 taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta
    11221 aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa
    11281 gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct
    11341 cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt
    11401 gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata
    11461 tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg
    11521 attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga
    11581 tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt
    11641 ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag
    11701 attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac
    11761 aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg
    11821 gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa
    11881 tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag
    11941 cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg
    12001 gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc
    12061 cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg
    12121 gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta
    12181 gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg
    12241 atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg
    12301 cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa
    12361 ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc
    12421 caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc
    12481 ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga
    12541 cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag
    12601 cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa
    12661 aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta
    12721 gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt
    12781 gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt
    12841 aattgacttc attccatatt cttccacgta taccatactt ttgaacagca aatattcttt
    12901 actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa
    12961 taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata tttagttata
    13021 tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg
    13081 aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa
    13141 ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta
    13201 actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag
    13261 agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca
    13321 atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac
    13381 tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa
    13441 ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc
    13501 taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca caaactgctg
    13561 taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga
    13621 caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct gtaaaaaatt
    13681 gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag
    13741 atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga
    13801 acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt
    13861 taactttatt catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa
    13921 tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt
    13981 tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt aattattttt
    14041 tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt ggttttttat
    14101 tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact tacttaactc
    14161 aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat tttcttgtgt
    14221 tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct acactttgct
    14281 ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa ttaagagaaa
    14341 aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa taccttagtc
    14401 aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc catttgcaga
    14461 taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag ttatttcgca
    14521 aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt acagatcagc
    14581 atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac aatacagtgt
    14641 tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt tatgagaaca
    14701 aaacaaagag gatgtgacaa aattcttgtc acatcctctt attccaaata aatggtattc
    14761 aatcagctaa ctctcggtat tagctcagat caaagcgctg atttcacaac ttctttattt
    14821 taatctcttc ggttgccgcc gaacaataag atcaagcgca acagctgcag gaatgtcgaa
    14881 agcgctgctg caacataagt cagtgctgct gcaaataata cgtgtcttgc catcggtact
    14941 tcttcttccg tcagtagtcc gccttcactc aaaatttgga ttgctcgccg agacgcgtta
    15001 aattcgactg gtaatgtcac caattggaaa agtaatgcta aagaaaaagc gaaaatcccg
    15061 atattgatta acgtagagtt ccagctaagc aatacaccta ccataatcaa ggggaacgaa
    15121 atcatcgaac caaagttagc tgctggaaca agtgctgcac gaatcttcaa tgggacataa
    15181 cccgaagcat cttgtaccgc atgtccgcat tcatgagctg ctacacctat cgcagcaact
    15241 gaagtagact gtgcagttgc ttgagacaaa ctcaacatct tattacctga attgtagtta
    15301 tctgtgagat ctccggcaat ttgttgaaca ccgacattat ttatctgttg actttgtaat
    15361 atatattgtg ctgcttgtgt accagtcaca ttgtttttgc ttctcacttg atcgtatttt
    15421 cggaaagtac tattgacgta cgctgacgca attccggaaa taactaatcc aagaatgacc
    15481 aaaagaaatg tcggatctaa aaaaccatac attccataac catacatttt cgtttcactc
    15541 cttttcgttt actttatttt atcattagta gtaggaaaag aatgtgaaaa gaccttgaat
    15601 attctttaat tctttttcac aaattccgat ttcagtttca tcggtccaaa tccttctacc
    15661 ttgcaatcga tattatgatc gccttctact aaacggatgt tcttgacttt tgttccttgt
    15721 ttaatggcac ctgacgcacc ttttactttc aagtctttaa tcactgtcac gctatcgccc
    15781 tctaacagac ggtttccatt tgcatctaat accactaatc cgtcttctat gacagtttct
    15841 tgttcttctt tgttccattc gttcccgcat tctgggcaaa tcaacaagcc tcgatcttca
    15901 tatgtgtact ctgagccaca ttctgggcaa tttggtaatg tcatcttctt tctcctttaa
    15961 aatctttagt gagcactatt gtaacggaaa aagaagatct tgaaaagata catttgtgat
    16021 ttttactaca aaaggcacgt tttcgaacga agtatcattc tgtttttctg ctcttatacg
    16081 attggcatat atcttggatc tctttataga tttcctctac ttctgctaaa tcttttgcat
    16141 tcgcaccact tgctaatgga tggcctcctc catgatggcg tttagccaat tcattgatga
    16201 tcggcccctt agaacgtaaa cgtacgcggt aataccctga aggctgctga acaaaaatcc
    16261 cccaagccag cacttcgtca atcactccag gaagtgaaac gatcgaagct gtctctgaat
    16321 catcgattcc atatttatca agaatttctt gcggaaggat tattcttcct gcaccatttt
    16381 catccacctg gatattttga taaagataac cggaaagttt agcgactttc atcggcattt
    16441 gttcaatctc tctgttcaaa gcacttgcat caaatggata atttctcaat atcgaagcaa
    16501 tctctaatgt acgggacgtc gtggagggat acaaaaagcg ccctgtatct ccgacgattc
    16561 ctgcataaag taaccgtgca gccttaacgt ttatctttaa agattcagga aacatcaaag
    16621 caaattctgc aatcatctca ctacagctac ttgcttttgt atttacccac acaagatctc
    16681 cataagggtc atcattggga tgatggtcga ttttgatcag tttttctgcc aactgatatc
    16741 tttgatcact gattctgggt gaattagctg tatcagtcac gatgactagc gctccttggt
    16801 agaagacatc ttcaacttct tgcatttctg ctaaaaagcg aagcccttca atcgtttcac
    16861 ctacttgacg tatttctttt tctgggaaac tttcccgtaa tagttcggct aatcctactt
    16921 gtgatcctaa tgcatcagga tcagggcgca aatgcctgtg gataatgatt ttatcgtatt
    16981 tttttattgt atctactatt tcttgctgta ctgtcatttc actcctgctt tctaagaacg
    17041 ttccattact tgacaaacaa cgattgcttt ggaaacgatg gtgttttcaa taaacacttc
    17101 gatatccagt ttagcggaac gtctgccgat ttccaataca cgtggacgaa tgtctaattc
    17161 gctttccaat tggatcagcc gcaaataatg taggttcact tgttcaatca agatatttcg
    17221 tttttggttc atcaccattg ttttttgtgc gacatcagaa atgatttcgc taagtacgcc
    17281 aaaagaaatg gttccaacgc cattgaccat ttgtggtgct actacgaatt tgaacttagg
    17341 gttttccaaa cgatttcctt cacggtcagt atcaatcgta tggatatttc cagatatctg
    17401 atccgaaatc gtatctgcaa tctgcggttg tcgctggaca agctgcatcg ctttcattac
    17461 atcttgccgg gtaatcaagc cttccaatgt caagtcatcg gaaacgaccg gcatgacttc
    17521 tagcccatcc cagatcattt gatggctaac agaggcaata ctcattgttt tctttacact
    17581 gattggttct ttcgtcatca cccgatcgat gatctggttt ggcgatttcc ctaaaacatc
    17641 ttttgccgta acaatcccca tcaaacgatg atggcggttg acgactggaa aacgcgaatg
    17701 ctgcgtttct tctgacaaac gctgataatc agaaatcgta tctgtctgct gcaaataatg
    17761 agtcttttca gctggcatat agatatcact gacaagcatg atatcttttt tgatcaattg
    17821 gtcgcttaac gctcggttga tcatggttgc gactgtaaat gtatcataag aggtgcgtaa
    17881 gatcggcatt tcaagatcat cagctagctt tgcaatctca gcagtcgtat tgaatcctcc
    17941 tgtgatcaat actgcggcgc catcttccaa tgctaatttt tggacttccg cccggttgcc
    18001 gacgatcatt agtgatcctg gcgtaatata gcgttccatg gcatgtttag tcatcgcgcc
    18061 gatgacgaat ttattcaaga ccttgtctaa tcctgccgcc ccgcctaaaa cgtccccttc
    18121 aatgatcctg acgacctcgc caaatgtcaa acgttcaata tgttttttta atttgcgttc
    18181 gatacggatg gtccctactc gttgaattgt tgaaactagt ccgacatttt ctgcatcttt
    18241 gattgcacgg tacgctgttc cttcactgac attcaattct tttgcgatgc ttcttacaga
    18301 aattcgattg ccaacaggta aactttcaat atattctaaa atcaaatcat gttttgtcgc
    18361 cattttctgc ctacacttcc agcgtttccg agacgctcat tatttttcct tcactatctt
    18421 ccagtaactc tacaaaagat tcgggatctt gttcaatgac agggaaagta ttgtaatgga
    18481 ttggaactac tttcttggca cgaattgttt gcgcagcctt tgctgcatct tctggcccca
    18541 tcgtatagtt atcgccaatc ggcaagaaag cgacatcaat atcgaattct tctgcaagca
    18601 atcctagatc actgaaataa gcagtatccc ctgcatgata aatcgttttt ccttctgctt
    18661 gaataataat tccgctaggc tcgcccatat actgaacgat accgtctact tcatagcttg
    18721 aactatgctg tgcatgtacg aattttacca cgccgaatgg aaacgcatgt tttccaccga
    18781 tattcatacc gaatgaatct actcctctgc tgttcgcata atcagcaatc tcaacaatac
    18841 tgataattgt ggcttgattt tcttttgcaa taggaatcat atctcctatg tggtcactat
    18901 gaccatgagt aatcagcaac caatctgtct ttacttcttc tgcagttaag tctgtcaatg
    18961 gatttcctgt aataaacggg tcaatcatca cgcgttggcc attttccatt tcaattgtta
    19021 aacaggaatg tccatgatac gtaattttca tattttacac ctcgcgtttt ctctgtatct
    19081 tttttattaa aaacgatttc atcacttgca catcaatgaa aaaatatagc tgctaaatat
    19141 attttcattg gattgatagt acagtatgta caaagcaatt gtaacagata atacaggatg
    19201 caccaagtac caagcagata taatctcaag tttgaggtct ctgctctgct tagcttctct
    19261 tctcgtccct taactaatta aatcagcgta aataggatgg acgatctttt ctaaatcggc
    19321 tacttttatc cgaaggcttc tcccgatttc accgcttgac acaattactt cttctttttc
    19381 ttttaaagaa ctgtctgcta aaaaacaata atcaggatgt gcgagtttta ttccaatagg
    19441 ggtgtttgct ccgtggggat atcctgtatg cattaataca aaatccattc caggaaaacc
    19501 aactttacga tctttcgtca cttgtctcgt ttttttgtaa tctaagtgat cgttcaaagg
    19561 aatcaatgcg atgatcaccc cagttttgtt tcctttcact acgagagttt tacatatcaa
    19621 agctggatca atccctcttt tttgtagttc ttctgtgaaa gattcttcta ccacatgact
    19681 aaaatcaagt ggttgatagc tgatcttttg ttcttctaaa taacgttcga cttttgtttg
    19741 actcatcatt gattcactcc ctttattcta tcgtttatta taaagcgagg gatgctgtat
    19801 tcaaaaatta tttgtttttc gcaaaaaaag agcaagacga gcactctata gagaggttca
    19861 tcttactctt tttgtttatt ttttatttcg acgttgagta gcttccataa tgactggcaa
    19921 gatcactggg cggcgttttg tttgttcaaa taagtaacgg cctaattgat ctcggatgtc
    19981 ttgctttaat ttgctccact caaaatcatt gctttctaaa tgtttctcaa cgattgtttc
    20041 aatcatattg ctgctttctt tcatcagatc acggctagct tttacgtaaa cgaatccccg
    20101 tgaagtaatt tgaggttttg aaatgatttt cttttctcgc cgactgatag tgacaaccgc
    20161 tacgaaaatc ccgtcttctg acaagatttt tcgatcacgt aaaacaatgt ttccgatatc
    20221 acctatgcct aatccatcaa tcattacatt ttctgcactt gtagttcctg caacactcat
    20281 acgcttgttc ttatattcca gcacgtctcc gcgtccagta atatagatat ttttgtatgg
    20341 catccctact gcatgagcca aatctgcatg agcagctaat tcacgatact ctccttgaac
    20401 tggcacaaaa tatgttggtt tcatcaaatt gatcattaat tgcaagtcat taggattggc
    20461 atggccagaa acgcgcatat tctcagagat caacttcact gttccaccag ctcgataaat
    20521 gatatcttcc gttttcgcca cgatggtttc catcgcgatg cttggcgtag tagtgatata
    20581 aaccaaatct ccttcttcaa tcttgacatt acggtgtgta ccgtttgcca ttttttgcag
    20641 agacttgatg ggttcaccca ttcggccagt ttctaaaatg atcagttctt ctggatcata
    20701 gtttttcatg tctttcaacg taacaagcag atcttcactt ggtaattgaa gtttttctaa
    20761 acgcatcgct gtgcgaataa ttcgttcgaa atcttgacca gtcaagacaa cttttcgttg
    20821 tgctttttct gctgcatcca atacttgttg cactcgttgc aaattacttg ctacacaagc
    20881 gacgatgata cgcccttccc aatatttgat cgtatcaaat acttcatctg ctatttgggc
    20941 ttcagaagct acaggctgag gattttctgc atttgccgat tcacttagta aggctaagac
    21001 tccttctttg ccgatttccg ccaatcgtgc ataatctgtt tgatacattg ggatagccgt
    21061 ttggtcaaat ttaaaatctc ccgtataaac gatactacct tcttttgttt tcagattgat
    21121 ccccactgaa tctggtatcg tatgagtggt acggaaaaag ctcactactg tttcaccaaa
    21181 gtctatttct gtatgctcat ctaccacatg gaaatcctta aatcctttgg ctgcttcatt
    21241 acgactgaca ttcaattttg ctaactcgat cgtcaactct gtcccgaata ctggtacaga
    21301 aatttttgac aacaagtaag gaagtgcccc gatcgcatct gcatgtccat gtgttaaaaa
    21361 gacaccagct acacgatcaa tgttttcttc taaatacgta aaatcaggga ttacgacgtc
    21421 gatccccaac aactcatttt caggatattt tagcccacaa tctaaaataa aaatatcctc
    21481 gcccacttct gcgacataca tgttttttcc attttcacga acgccgccta atgggataat
    21541 tttaatggta ctcacaaact tcacctcttc ttatgacatc caagtttaaa gatgtcgatt
    21601 ttcttcttta tctaaatata tgagccattc caatgaagca tatgcaaaat cactatttct
    21661 caacttatgg agaatcagca ggaaaattac cagcacatcc gcgttctgac agttggatgg
    21721 gcattttatt ttggatatac ttcattaaca aaatggatct tttgcgaaac gctatagcca
    21781 gaatacttat ataagtattc agctgttccc cattattata caacaaataa actaaatcgc
    21841 tagtatcagc aattgttaga acaactatgc acgtaaaaat ccctctgtag catctaggat
    21901 ttcttttgct attcctcttt catttcttct tcgtcggttt gaaacatact tttttcattt
    21961 taccacattt tttgcaataa ctatagtgac tttttatata tacagaacct catgttgatg
    22021 gtgtgcaaac agcataagat ttaagctagc tttaagatag gttgtctatt atcaaatgca
    22081 tctactagga gggatgcaaa atatgaattt tatgaaacgt gcatggaaaa gtacaaaagt
    22141 aaaatgggga cgttcattgt tattgtttgc cgtgttcaca gccattctta tttttgtgct
    22201 agctggcctc acgattcgaa gcgctgcaga agaagcagcg aatcaagcac aaaaagaagt
    22261 tggagcaaca gtgactttaa gtgccaatcg agaagcttca tttaaaaaac aggaacaaac
    22321 ttcttcaagc ggttctacca ctgaagaacg tccagatcca ggcagttttt ctcttacacc
    22381 agtttcctta acggacgcgg aaaaaatagc tgaattagat aatgtaaaga gttattcctt
    22441 tgaaagttcc gcctcagcta tggcaaaaga tggaataact gccatcacta gttctgattc
    22501 aagtgaagac agttcagaaa ctactgatgc aggcggtgaa cctgctggag gaatgggcgg
    22561 gaatccgcaa atgactcaag cagactttca aatcagtggt gtgtcagcaa ctgcacaaaa
    22621 tagtaatttt tctgatggaa cagcaaaaat cacggatggt gaaggaatag atgacagcga
    22681 caaaggcaca aacaatgtag tcattgattc tactctagca gaaacaaatg acctggcagt
    22741 cggagatact ttcacaatca cgagtactga agatgaagat accacttatg aaatgacgat
    22801 caaaggaatt tatgaatcca gcgaaacaag cggcagtatg ggtatgaaat tcaacttcat
    22861 gaatccgtcg aatacacttt acacttctta cacttttgca aatgaactaa atgggacatc
    22921 tgacgataac acgatagatt ctgctattta cactctatct gatccagata aaatggatga
    22981 gtttctcaaa gaagctgaga agctgatcga cacagataca tttagcttac aatcaaatga
    23041 ttccatgtat cagtcaatgt tggaaccttt aaacaacgtg gcaagttttt ctaaaaatgt
    23101 tgttttacta gtggctgtag ccggtattat catacttact ctgatcatta tgattaccat
    23161 tcgggagcgt cggcatgagt taggcgtctt gctatcttta ggagaatcca gaagtaaaat
    23221 cattctccaa ttgtttaccg aagtggctat ttgtatgatt cttgcattag gagttgcttc
    23281 gcttagcgga aacgtcgtcg cgaatgccgt gggacagcag ctacttgacc aacagacaga
    23341 aaccacgaca caaaaccagc aagctcctgg cggtggacaa atgccaggta acagacaaga
    23401 acaaggcggt caacgcgatg gtcaacaagg aaccacgaat aatccatttg ctgtttctga
    23461 tcaagtcagc gaattggaaa tcaatgtcaa acctgcacaa ttgggactat tagccggagt
    23521 agcatttgga atcagtctgt tttctgtctt tttagcttct atagggatat taagattaaa
    23581 cccaagaaaa atcctattaa actaaaggag gaatttgatg ttacggacag aacaattggg
    23641 attttggtat caagatccgc aagatgcgct ttataaagat gtagaactga atttcgaagc
    23701 tggaaaaatg tacgctattt taggagcaag tggttctgga aaaacgactt ttctttcgct
    23761 tatttctgga ctagatgcac caaaagaagg aaaggtatac tatcaagatc gttcactgaa
    23821 caaaatcggg ttaagaagtt atcgtaaaaa tgatgtctcg attattttcc aagcttacaa
    23881 cttgcttcct tatttatcgg ctctagacaa cgtcatagca gcaatggaaa tcactaagac
    23941 caagcaagag aattatcgac aagttgcttt agatcgactg gctcgtgtag gaattgatga
    24001 aatattagca aaaaaacagg tgaccaaact ttcgggaggt cagcagcagc gagtcgctat
    24061 cgttcgtgct atctgctgtg aacatcaact aatcgtagcg gatgagccta ctggaaatct
    24121 agatgaaaca acttcacaag atatcgtcaa acttttccaa gaaatcgccc atgaacaaaa
    24181 tcgctgtatt atcatcgtga ctcatgagat tgaagtagca aaaagctgtg atgaagtctt
    24241 tgaactaaaa aataaaaaat ttcaaaaaat cgaagtttaa aaaacaggat aatgatcaaa
    24301 taaaaaatcc gaaccatatc tgtctcgcag atatggttcg gattttttat ttgcttgatt
    24361 caattagtaa taagtggttt ttattttttt tcgtcttttc gagaaaactt gtctttgact
    24421 tcgtcgccta aatttcgagc tttttctttg atgtcagcac ctagtgattg tgctttccct
    24481 tctgtttctt tgctcttgtc gtctgttgct ttgccgtatg cttcttttcc ttcaccgact
    24541 actttatctt tcatatcttt agaacgtcct tttaaatctg ccattgtgct tcctcctctt
    24601 agtttgttta ttgatctacc ttcagggtaa catcaacaac agatacagac aaacagaacg
    24661 ctcgttttct aaaaaagata gttagagctg ttgattaaaa acttttttcg aaagtccccc
    24721 atactttgac ttctttttct tggaccatta tttctccttt agcgataact gtatcaatat
    24781 caagcgtttc tttatccaag atacacagat ctgcatcagc accaatttcg atttttcctt
    24841 ttttatccaa tttcaatata cgtgctggat tactggttat agctcgtaag gcgatttcca
    24901 aagggatact ttctttttgc acagcttctt tgatccctac tagcaatgcc ttggcacttc
    24961 ctactcctaa ccctaggaaa tgattgtttt catcaaaata aggcaagctt ccttgtccat
    25021 ctgaggtcat cgtgaaatta tctaaagaaa catcttcctc gatcaatcgt ttcagtgctt
    25081 tgctgaagcg aacttcacca tctgtcttct cccaaaaatc aggatcttcg ctagcagtga
    25141 agtcaatcgt gccaccacgt ttggcaaatg ccacgcaagc ttcaaataat tctggtgtgc
    25201 gatttgcatg agtaggcaaa aattgtgtga tcggaatttc tgtttcttct acgattcgtg
    25261 tcaatagctc tagctttctt tttcctccgc ctaagtggac attgatgatc ccagcttttc
    25321 cggataacat gcctcctaca cgagcatctg ctactgcacg agcgaactct tcaaaacttg
    25381 gctgagaaga acggtgatcg gatactgcga tttctccgat tccgataatt ttatcaattg
    25441 tcaaaaagtc tttgatgata gaatctgtta ctgtttttac cggcaatcgg tacgaacctt
    25501 cgtatacata agtggagatt ccttctgctt ccagaccttt cgctttgctg atcaacgcag
    25561 tcatatctct accttctccg tctgttccca aacaaccgac aactgtcgtt acaccagctg
    25621 tagtcaattg gctcaatgtg acttctggcg ttcgattttg ataaccgcct tctcctcctc
    25681 cacctaaaat atggaaatga cagtcaatga aacccggggt tagtactttt ccctcacctg
    25741 aaagttcttc tacttccact ccttcgtagc cgccttctag ctgttcttct acagccaaaa
    25801 tcttcccacc ggcaatcagt acatccattt ttcctaagta atctggtgca aacacttcaa
    25861 tttgtcgaat gattttcata catttttcct ctttctttaa gtataactga tgataggatc
    25921 ttaaaaaata atccaaacga taggatcttt actcctttga ttttaccgta aaatgacggt
    25981 tcttggagtc cttatctact gtttggatta cttttttatt tacaggtctc cagtactact
    26041 gcagacctat tgcaatggca atcatgatga aaatagttcc taaaacaaac aggaatcctt
    26101 gcatcttgat ttggaatctt gcccattttg cccattcgat ccgtgcaaca cctagcacac
    26161 caatcaagct ggctgatacg ggtgtaaaag cgtccataaa acctgaacca agctgataag
    26221 caagaactgc tacttgtctg gatacaccta ctaaatctgc cagtggagcc atgattggca
    26281 tagtcaaagc tgcttgtcct gaatttgatg taacaaccaa gttgaacaaa ctttggaaga
    26341 tgtacatcgc ccaagcacca atgacagctg gaacaccggt caacgccgtc ccgatcccat
    26401 gcaaaatcgt attcaaagct gaaggaacag atgcatcaga tcctcccaag acaagaagaa
    26461 tccctttggc catacctact acgattgctg taccagctaa atcagctgca ccagattgga
    26521 aagaagaagc gatgtcattg actttcattc catctaattt gaaaagaaca gcaatgattc
    26581 cagcaactaa tcccatcaca aagaattgag aagcgatttc tggaatgtaa tagccttttt
    26641 gagtcacgcc ccaaacgatc cagacaagga caactaacat ctctaataag atcaatttat
    26701 gcccaagcat gaatttttta ttttcatcag atgtcttttt gatatgagaa cggaaataat
    26761 catctgattt gtaagtcaac gaactttctg gttttttacg gattttttct gcatatatca
    26821 tcatataacc agcggctaat gcagtaacaa ctacccacat aatcaatcgg aatgtcgcac
    26881 cagacaacac ggggattcca gcaatccctt gagccaccgc tacgctaaac gggctcatcc
    26941 aagaggtcgc atttccgact tgagatgcga cataagtcac ggttactgct acgatcgagt
    27001 catatcctaa cgcaatgaca aatggaacca tcaccattga gaatggaatt acctcttctg
    27061 ccatcccgaa tgtagcgcca ccgaatgaaa aagcaaagaa taatagcggt aaagctaaac
    27121 gctccagtcc cttagtttta ctgataaacg catagatacc tgcgtcgatc gctccggtac
    27181 gcataataat accaaaagca cctccaacaa caaggatcaa tgcagcgatc cctacagcag
    27241 atccatactt atctccagat actagtcctt caaaaacaaa atttaagaaa ccaaagccgc
    27301 caaaatcatc tgttccccat attttggctg ttttatgcaa ttttttagaa gtatcataga
    27361 tattttcgcc gtactgatcg taaagcacat catctgtcaa ggagatttca tctagttttt
    27421 cctgcgtaag atttttttct ccgtcagcaa tgactttttc caatcctttt tccggaacat
    27481 ccagtttttc acgttctgct ggatgatctt gcagttcttc taattgatca aacacatacg
    27541 acttatccag ctcgtaagca taacggaagg aatcttgacg taaaacagta cgagtacttg
    27601 tttcgccatt tgcatctttg tattcaatat cttgcgtgct gaactttcct gctggaacga
    27661 caaaagtcag tatccatgca aagatgacta caccaaaaat aatgacatac gtatggggtg
    27721 tcgataattt agtgaagtcg cgtttcggtt ttcctgaaga atttgttttc ttcaaattta
    27781 tttcttcctt tcttttttca gtaattttga tgaataaaaa atggataatc aaatgaatta
    27841 tacagtaaaa atgacatttc cacatgaata atttattaga aaatcacttt tttctatttt
    27901 gtttttttct cctatgtatg attatttttt tctctttcca tttatattaa gtattttatt
    27961 tctctgtttt tccttaaaat tcttttttta tttttagatt agagaaaatc tcgtcctgtt
    28021 tttttcattt tacaatcatt gaaaaattga atttcattgc aaaaaaaata ataaactctt
    28081 ataaatatcc attatctatc cttcaatttt tgtgt
//
LOCUS       DACDIP010000032        27747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDIP010000032 DACDIP010000000
VERSION     DACDIP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.032"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..107)
                     /locus_tag="IV257_001578"
     rRNA            complement(<1..107)
                     /locus_tag="IV257_001578"
                     /product="16S ribosomal RNA"
     gene            26..169
                     /locus_tag="IV257_001579"
     CDS             26..169
                     /locus_tag="IV257_001579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814694.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(787..2283)
                     /gene="lysS"
                     /locus_tag="IV257_001580"
     CDS             complement(787..2283)
                     /gene="lysS"
                     /locus_tag="IV257_001580"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8814695.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2394..3398)
                     /gene="dusB"
                     /locus_tag="IV257_001581"
     CDS             complement(2394..3398)
                     /gene="dusB"
                     /locus_tag="IV257_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8814696.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3399..4286)
                     /gene="hslO"
                     /locus_tag="IV257_001582"
     CDS             complement(3399..4286)
                     /gene="hslO"
                     /locus_tag="IV257_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8814697.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4503..6614)
                     /gene="hflB"
                     /locus_tag="IV257_001583"
     CDS             complement(4503..6614)
                     /gene="hflB"
                     /locus_tag="IV257_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8814698.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6704..7249)
                     /gene="hpt"
                     /locus_tag="IV257_001584"
     CDS             complement(6704..7249)
                     /gene="hpt"
                     /locus_tag="IV257_001584"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8814699.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7249..8694)
                     /gene="tilS"
                     /locus_tag="IV257_001585"
     CDS             complement(7249..8694)
                     /gene="tilS"
                     /locus_tag="IV257_001585"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8814700.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8814..9284)
                     /locus_tag="IV257_001586"
     CDS             complement(8814..9284)
                     /locus_tag="IV257_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8814701.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9330..9755)
                     /locus_tag="IV257_001587"
     CDS             complement(9330..9755)
                     /locus_tag="IV257_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8814702.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9822..10091)
                     /locus_tag="IV257_001588"
     CDS             complement(9822..10091)
                     /locus_tag="IV257_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8814703.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(10102..11697)
                     /locus_tag="IV257_001589"
     CDS             complement(10102..11697)
                     /locus_tag="IV257_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8814704.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11712..15233)
                     /gene="mfd"
                     /locus_tag="IV257_001590"
     CDS             complement(11712..15233)
                     /gene="mfd"
                     /locus_tag="IV257_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8814705.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15250..15810)
                     /locus_tag="IV257_001591"
     CDS             complement(15250..15810)
                     /locus_tag="IV257_001591"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8814706.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            16163..17137
                     /locus_tag="IV257_001592"
     CDS             16163..17137
                     /locus_tag="IV257_001592"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8814707.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17307..18707
                     /locus_tag="IV257_001593"
     CDS             17307..18707
                     /locus_tag="IV257_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8814708.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18785..19507)
                     /locus_tag="IV257_001594"
     CDS             complement(18785..19507)
                     /locus_tag="IV257_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8814709.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19586..20092)
                     /locus_tag="IV257_001595"
     CDS             complement(19586..20092)
                     /locus_tag="IV257_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8814710.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            20215..22014
                     /locus_tag="IV257_001596"
     CDS             20215..22014
                     /locus_tag="IV257_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8814711.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(22082..23656)
                     /locus_tag="IV257_001597"
     CDS             complement(22082..23656)
                     /locus_tag="IV257_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814712.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23911..24420)
                     /gene="mreD"
                     /locus_tag="IV257_001598"
     CDS             complement(23911..24420)
                     /gene="mreD"
                     /locus_tag="IV257_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8814713.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24424..25278)
                     /gene="mreC"
                     /locus_tag="IV257_001599"
     CDS             complement(24424..25278)
                     /gene="mreC"
                     /locus_tag="IV257_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8814714.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25433..26071)
                     /locus_tag="IV257_001600"
     CDS             complement(25433..26071)
                     /locus_tag="IV257_001600"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8814715.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(26072..26722)
                     /locus_tag="IV257_001601"
     CDS             complement(26072..26722)
                     /locus_tag="IV257_001601"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8814716.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26735..27634)
                     /gene="rsgA"
                     /locus_tag="IV257_001602"
     CDS             complement(26735..27634)
                     /gene="rsgA"
                     /locus_tag="IV257_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8814717.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
BASE COUNT         7618 a         5985 c         5049 g         9095 t
ORIGIN      
        1 tctttttccg gtggagcaag ctccggtgga aaaagaagcg ttcgacttgc atgtattagg
       61 cacgccgcca gcgttcgtcc tgagccagga tcaaactctc ataaaaagtt cgaacaatct
      121 tgcgattgtt aagctcaatt tgtttgctag catattgctt gcttgttaaa aatgtttgtt
      181 gtcttgaatt gaatcaagac gccctacaca tttggtttgt cttactttgt tcagttttca
      241 aaggtctact tcatcgagag tgtttcccga ttcatttttg gctgccgtag caacttttat
      301 atcatatcag agagacaagc gattgtcaac tcttttttaa aagttttttt cgatttcgtt
      361 tttcgaaatc tttcggcaac tcatttatca taacttatcg tttgttattt gtcaagaact
      421 tttttgaagt ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag
      481 ttatcttatc atgtcgttag taacatgtca ataacttttt tgttatgttt ttgaataaat
      541 attttcttct gtcttttgga caagtaataa tataccaact aaatttagct tggtcaacga
      601 attttttgta ttttttcaag tttttttcaa taaacgatta ttcataactt ttctttcttt
      661 atcgttcgtt ttcgtcttct ttaatgtcta gaatagtcgg cattagcttt tgaactttcg
      721 ttattattta ttcctttcta cacagcaaaa gctgcctaag gaattcctta gacagctttt
      781 attcgattat ctcattgttg ggaatagcaa aacatcccga attgattgtg catcagttag
      841 caacattact aatctgtcga tcccgatgcc tagaccacca gttggcggca taccgtattc
      901 taaggcttcc aagaagtctt cgtctacacc gtgagcttcg tcatttccta gttcacgttc
      961 tttttcttgc gcttcgaatc tttctctttg atcaattgga tcgttcagtt ctgtaaaggc
     1021 attagcaaat tcgcgcccca ctatgaatag ttcgaatcga tctgtgaatc gaggatcttc
     1081 tggatttttc ttcgctaaag gcgaaacttc gactggatgt ccgtatacaa acgtcggttg
     1141 tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg ataacatgtc cgacacccat
     1201 agcttcggtt acttctacat tgtgtttttc ggctaaagct aatgcttctt ccaatgtcat
     1261 ttcttgccag aagtctacac cagtttgttc tttgattgca tctaccatat ggatgcgttt
     1321 aaagtcactt tctaagtcga cagcttgtcc atcatatgtg atttttgctg ttcctagtac
     1381 tttttcagct gcgttacgga taataccttc tgtcagattc attacatcat tgaaatcagt
     1441 atatgctgtg tatgcttcta acatcgtaaa ttctggatta tgtgtagtat caattccttc
     1501 attacggaaa acgcggccga tttcataaac tttttccatt ccgccaacaa ttagtcgttt
     1561 taaatgaagt tctagcgcaa tacgcaaata taaatcaatg tccagtgcat tatgatgcgt
     1621 gataaatggg cgtgcggcag ctccacctgc ctcattgtgc aatactggtg tttctacttc
     1681 aatatagcca ttgccatcta aatagcgacg gatttcgctg ataatttggc ttcgtttcat
     1741 aaatcggtca aagctttcgc gattactgat taaatccaag tagcgttgtc tgtagcgttg
     1801 ttcaatattc gttaagccat ggtatttatc tggcagtgga cgaagtgctt ttgacaaaat
     1861 cgtgatttct gaagctttga tagaaacttc gcctgtgtct gttttcatga tttgtccagt
     1921 tacaccaaag aaatctccaa ggtctgcatg tttgaatact tcataggcct catcacctac
     1981 ttggtctttt cttacataaa tttgaatttg gccttcacga tcttgtagat gcgcaaatcc
     2041 agctttccct tttcctcggc gagtcatcat acgaccagct acactagcgg tcaatcccat
     2101 ttctgctagt tcttcttttg ttcttggatc aaatagttca tgcaattctt ctgagttgtg
     2161 cgtgcgttca aatcgttttc caaatggatc gattccatct tcacgcaatt gttccatttt
     2221 ttgacgacgt acaagcattt gatcatttaa atcttctact tgatgttgtt gttcctgtgt
     2281 cacaaaagtt cctccattct catgttaatc tctttcctat aatgccaatg aaacacaaaa
     2341 aaagcaagcc gaacttggaa tttcggcttg aaaattcacg aaaaacttag aaatcacaac
     2401 gtttgtttca atagtacttt ttccgctttt tctacaaatt gatccagcag gtcattgatt
     2461 tcttgctgtt tttctgtctg attgattgct actttcactt tcgctgctcg aggaatccct
     2521 tttaaataat aagccgcatg tgttctgaat tctctacaag ctactttttc tcctttgaga
     2581 tctgctaagc gttgtaaatg aagtttagct gtagcaattt tttcggctgg tgttggttca
     2641 ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt gaatcatcca tggattgccc
     2701 agcgctgcac gaccaatcat cacaccgtct gcaccgacat agtcaagcat ccattttgca
     2761 tcttctggcg ttttgacatc cccgttcccc ataaatggga tcgtcaaatg ttttttcact
     2821 tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt acgtccatgc
     2881 atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac agcaaagacg
     2941 tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac tgctgaagaa
     3001 acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc tcctgcttct
     3061 gctttgatta ctttatttac tggacagccc atattgatat ctattatatc ggcagctgtg
     3121 ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc aaatatctgt
     3181 aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt tttgtttctg
     3241 aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc aaattctttg
     3301 acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac gacacgatta
     3361 gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc taaggcttct
     3421 ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca gaaagaacaa
     3481 accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc acctaacgcg
     3541 ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac aggcattttt
     3601 tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc tggtgtctgt
     3661 ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc gatcttgctg
     3721 attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc cgtcttgatg
     3781 ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg ttctgagata
     3841 gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt ttgcccagaa
     3901 aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt tccaactgct
     3961 ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg atttttgatg
     4021 taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc tggaccatcg
     4081 ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag tagcagacta
     4141 ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg acgtctttgt
     4201 gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc gtcgtgagca
     4261 agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt tttaaactca
     4321 tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct tttaaaaata
     4381 attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata aagaaatctc
     4441 ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg acctatattt
     4501 ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc tgaatcatgt
     4561 tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg tttttcttcc
     4621 gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa agttttcgct
     4681 tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc gtgttcaaac
     4741 aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc agcaatcagt
     4801 ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc ttccatcaag
     4861 atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc ttttgtttga
     4921 ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg acctaaacga
     4981 tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg ctcaaagtca
     5041 ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg tccaccaagt
     5101 aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc ttcttttggt
     5161 aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt gtggacgacc
     5221 cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata agcaaccatt
     5281 tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat cacgcgatct
     5341 tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc agctactaac
     5401 gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt ctgttgagcc
     5461 acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac tcgcaagatt
     5521 gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa acggcctgga
     5581 cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac gattactcct
     5641 tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg ttcgcgttca
     5701 tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat ttcatcaatg
     5761 aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac acgacttgct
     5821 ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa cggtactcca
     5881 gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg tccttctaaa
     5941 agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc tttcagaaat
     6001 tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc agaaaaacgt
     6061 acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt catgacacgg
     6121 ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa aaagatgatg
     6181 attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct tgattcttct
     6241 tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct caatgttgta
     6301 tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga acctaagata
     6361 gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt gatcttatat
     6421 acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag ttgttctgtg
     6481 aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat gaaataaacg
     6541 accattacca tcgctaagat tacgaggaca taatacaagg catttttcat catgccgttg
     6601 ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt tttttatcaa
     6661 tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt gattgataaa
     6721 cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca taatccaagc
     6781 cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct tcgatattta
     6841 ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt gctttgcggt
     6901 atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata tcttcaacga
     6961 tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt acttcccctg
     7021 acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc aaatacgtat
     7081 cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca cctactacta
     7141 ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg caacgaactt
     7201 gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc aggttctcct
     7261 tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat cagtttcttc
     7321 acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc ttttggcatc
     7381 tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga taaaatatct
     7441 agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt ttcttttttc
     7501 gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag accatgaatc
     7561 tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt ctgtttcatt
     7621 caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct ggctagttgc
     7681 ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta tccaattatt
     7741 gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa tcgactccat
     7801 ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa aggagtaaaa
     7861 aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa atcgaaattg
     7921 ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt gaatgacttg
     7981 gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct gtgtattttc
     8041 ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat ctaacaattg
     8101 gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat ataggtcttc
     8161 tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt gtacttcttt
     8221 gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca ccgtctcgat
     8281 ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt ccatcacgcg
     8341 agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt tggctggatc
     8401 ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc gaagataggc
     8461 agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac ctaaagaaaa
     8521 accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat caacaccacc
     8581 tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat tcccgtgccg
     8641 aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt tcatcctttc
     8701 cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac aattaacctg
     8761 ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc tttttagctg
     8821 cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga agataagcgg
     8881 tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt ttgagctgaa
     8941 ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc acggggatct
     9001 ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg ttcctgcgct
     9061 ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt aactgttact
     9121 tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga cacttcgcta
     9181 atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa atttgtgatt
     9241 cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa aatgttcctc
     9301 ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact agttgattga
     9361 tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac tttttcaccg
     9421 tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc atctttcagc
     9481 aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat ttcagtattt
     9541 gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa aatctgcaca
     9601 cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct gcgtcttcga
     9661 aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc atattccgta
     9721 tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa tctttttgat
     9781 tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc tagtgtttct
     9841 gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt ggttgattca
     9901 tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt caaaatatcg
     9961 cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat tcttccttta
    10021 tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt taaaaattta
    10081 tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca ggattttctt
    10141 accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa tcactgcata
    10201 aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag cagtggaacg
    10261 atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca gtactactgt
    10321 catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc aaaaagcatt
    10381 tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc ctaaaagggt
    10441 agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag tcaatccttt
    10501 taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct tgctttgagc
    10561 aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa ataatacaag
    10621 cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga gcagcaaggc
    10681 taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga ttttggctgt
    10741 ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag gcaggaatgt
    10801 cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac ctcgatcata
    10861 gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca aagcattttt
    10921 tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa tcgaaaccag
    10981 tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga acaatggaaa
    11041 ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa ggatcccata
    11101 tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt ggtacaccgt
    11161 ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac gaacgaactg
    11221 ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga aattccctcg
    11281 ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga tcagcggttt
    11341 caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga caaacaacca
    11401 caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt gctttcgctc
    11461 gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac cggataaagc
    11521 taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa aaccttcgtc
    11581 gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg ctatgaaaga
    11641 agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat gatccaaatc
    11701 attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac aaacttttgg
    11761 atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg tatcaccatc
    11821 tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc ttcgaataac
    11881 tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat atgcgcctcc
    11941 tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg cccaattgtc
    12001 aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc atcttctagt
    12061 tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc gattttctgc
    12121 cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc gatctcaaca
    12181 gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc gcttagcatt
    12241 tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc acctaagaga
    12301 ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga tcccagttct
    12361 gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat cttctgctgc
    12421 tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc tcgcaattgg
    12481 tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt tgcgttcggg
    12541 atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg accttcaata
    12601 aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc ataaccgatc
    12661 cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt ttctacccga
    12721 ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc ttcgcgaata
    12781 gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc aggcggtgtt
    12841 tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct cggaatcggt
    12901 gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg ttccttatgt
    12961 tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct aaattcaata
    13021 tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt acgcagttgt
    13081 tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc gatatttacg
    13141 gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa gatcgttgtt
    13201 ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc tcgaagcgct
    13261 acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg tttttctttt
    13321 tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc agagtagggg
    13381 aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata tcctttttct
    13441 gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat ttttgaagag
    13501 acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg aggcgctttg
    13561 gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat aaacagtttg
    13621 tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac ttctagcgtt
    13681 tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc tcctgctttt
    13741 aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct cgtttgtttt
    13801 ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc ttgaggaagt
    13861 tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat caatgcattt
    13921 ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc tttgcgtatt
    13981 ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca acggtccatt
    14041 tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata ttgaaaatta
    14101 tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa aaaggttgtg
    14161 gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg ctcagaaaac
    14221 acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc gatttcccga
    14281 tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga agggaaataa
    14341 tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc ttcttctgtc
    14401 gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa aaactcgcgg
    14461 tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat ttgctctatt
    14521 ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac ccagatttct
    14581 tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga atctatctct
    14641 acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat atcgataatg
    14701 cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac atagcccatc
    14761 aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg ccaattcaat
    14821 tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc gactggcaac
    14881 aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct ctcgcctcgc
    14941 gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg aaataaatgg
    15001 atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc ataatacaaa
    15061 tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc aaatagaagt
    15121 gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc ttctaaattg
    15181 ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc cattcctttt
    15241 cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt ctatgagata
    15301 ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat tttctttgct
    15361 gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc cgattcccac
    15421 cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga taccgttatg
    15481 cccgccggca cttccctttt gacggagccg gatttttccc actgccagat ccaaatcatc
    15541 gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg ggccgactgc
    15601 acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt ctccattatg
    15661 aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt gatgctcgcg
    15721 tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt atttggtacc
    15781 tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc taaaaagcag
    15841 cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc ttttttatac
    15901 ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa tcgttaataa
    15961 atgataaata aattaaaaat ttcttcaaag tttaatgaaa taattgatat ataaaaatag
    16021 tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg ttatatttta
    16081 tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg aaaaaacaaa
    16141 tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc atcaaaaagt
    16201 tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag ttactcaaaa
    16261 tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag gagacgcaat
    16321 tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg ctacttacgc
    16381 agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa aaccaggtga
    16441 aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg tcacacaaat
    16501 cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag atattttgac
    16561 ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt caggtacctc
    16621 tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg atgcacgtaa
    16681 cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt ggtcacatgc
    16741 aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa tcgatgaaga
    16801 agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta tcgagaaaaa
    16861 aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg ccatcttaga
    16921 tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg gattgaacga
    16981 catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag taatcgaaat
    17041 tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat taaaagaaat
    17101 tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga cggaaaacat
    17161 ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa ttttttcata
    17221 aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc gttccaccct
    17281 aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa aaatcgaaac
    17341 atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt tatacctatc
    17401 gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga ataaatgtca
    17461 gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct gataaaaaac
    17521 tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag ctcggctaca
    17581 aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga tggacttggg
    17641 gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt caaaaccacc
    17701 aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg aacaaagacc
    17761 ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt atcaagcctg
    17821 aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa agtgcggtaa
    17881 actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa gtaacatctg
    17941 aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat gtaaaagcta
    18001 cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg agttggctga
    18061 cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat gtaacagatt
    18121 taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca aaaagaggag
    18181 aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt gagacagaag
    18241 cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt gtgcaaggtg
    18301 gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat ctggagaacc
    18361 agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc gtttccggaa
    18421 agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa gaagatgcaa
    18481 ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac tactggatgc
    18541 cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa tacggtggag
    18601 acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt gtaatgaagg
    18661 aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa aaataatata
    18721 taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc taacaatgtg
    18781 ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct tctagcttat
    18841 ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc gtttgtggaa
    18901 tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct cgtacagcag
    18961 caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc agatcttctg
    19021 cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac ccatcgcgtt
    19081 caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt tctcccagtg
    19141 atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta cggttatctg
    19201 tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac gtgatttcat
    19261 cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc acattgtcgt
    19321 taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga ttcatagatg
    19381 gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc tgaaagactt
    19441 tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat ttactatgtc
    19501 ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta gtactatttc
    19561 acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca aaaagcacac
    19621 ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg aacataggtg
    19681 ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct gttgctgaaa
    19741 gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca cctaaaaaca
    19801 agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa aagaactcta
    19861 aaaatttggc gtagcccaga tggtcgatac tcaccatact tccaccataa ttaaaacgta
    19921 tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat cgcgaaactt
    19981 gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg actatcagtg
    20041 cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc ataagtcacc
    20101 tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata gagacttctt
    20161 ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt tttcatgagt
    20221 tattcattaa attggaatct agattctatt ttttccggag gcagtcattc tgatgctttg
    20281 aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg cgtaacaaaa
    20341 tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt gcaagaaacg
    20401 attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc tgccaatgtc
    20461 aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc tcgtctgcaa
    20521 tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga ttggaatcag
    20581 atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat tcgtcgtgat
    20641 ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc tttagatggt
    20701 ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat tcccttcgaa
    20761 caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat gatgggcgat
    20821 ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg gaaagaaaag
    20881 gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc tgactatgaa
    20941 cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt gaagaaagaa
    21001 acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt taattacttg
    21061 actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca agatgcacct
    21121 attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc agcatttatt
    21181 atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc tgcttttgaa
    21241 aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta ttgtactgag
    21301 ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt taatgaggtt
    21361 gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg ggatttacct
    21421 tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt cgcagaactg
    21481 atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat caatttgcta
    21541 gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg attcattttt
    21601 gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa gatcacagag
    21661 atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta ccatccatat
    21721 ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa cttcccatac
    21781 acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa gggagcaaat
    21841 tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac ggaagatctt
    21901 gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc aggtatcgac
    21961 atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat ctgatttcta
    22021 ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg attaatcatt
    22081 cttacatgct gacagcaaag tcaggggcaa accattgaac tgaaccaact tttacgcttt
    22141 cgcctggttg aggagcatgg atatattgtc ctccgcctaa agcaatcgct acgtggtaag
    22201 ttccgccttg tgacccccag aataacaagt ctccagcttt tgcttgagaa actgaaattt
    22261 ttgttccagc tgattcttga ggaactgtcc aaccaccgat gtcacgacca gttacttgca
    22321 tgtaaacata gcgtgtgaac cctgagcagt caaagccact tggatcttta ccgccccaaa
    22381 catatggagt accaatatat ttgtaagctt ctgctacgat tgctgcacca ttcacacttc
    22441 cagaaggagc tggagcaggt gttacaggtt ttggttgttc aggtgttggt gttggcgttg
    22501 gagccggagt actacttcct gtgccgtttc ctgtatcaac actttgatct gttgatggtg
    22561 ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga ttcaggcact gttgttgatt
    22621 cttctgttgc tgaagattca ggcactgttg ttgattcttc tgttgctgaa gattcaggcg
    22681 ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgattcttct gttgaagatt
    22741 caggtgttgt tgattcttct gttgctgaag attgtgtagc tgatgatgat tcttcagtca
    22801 ttgaagattg tgtagctgat gattcttctg ttgctgagct ttgtgcagta gatgaagctt
    22861 gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg
    22921 cagcttgttg acgagcttgt tctgctagac gttgttgttc acgaatacga gcttgctctg
    22981 cttcagcttc tgctttttga cggttcaagt cagctttctt gtcttcagca gttgcttgtt
    23041 cagcagctaa tgaagttttc agtacgttca aatcagcttg tttagataaa agatcgcctt
    23101 tttgagattc caaagcagct tggttttcag ctaattcttt tagtttagca tcgttttcag
    23161 cttttttgtc ttctacagct tttttatctt gtttttgttg ttccatcaag tcgttgtttg
    23221 cttttaccat cgttgtcatt gcttgaacgc gtccaatagc atctgctaat gaatcagcat
    23281 tcaatacagc atcgatgtag tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt
    23341 tttggattgt atcttcacgt ttttcaatac gttcttgtaa atctgcaatg tcttttacta
    23401 attgtgcaga ttcttgacgc aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt
    23461 tgatttcaga aacttggctt tctaaggcat cgatttgtga ttgtgcatct gcttgttgat
    23521 ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg
    23581 cggcagcgat cggtgatgct acggcagtca aggtcattga acataccatt actgctgata
    23641 ttaaactctt tttcactctt cattcctccg actggcttaa tttaataaat atctaaatat
    23701 atttaagtct ttcttaacga tataaaaatt gtaccatagc agtgtgtcaa cgatataata
    23761 gttatgttac aaaaatgttt caaaacaact tttaaaaatg acaaaactgt tcgtttcaga
    23821 aaatgatcat gaaaaagccg cgttaagtct agcatttctt aagataacaa cgattctttt
    23881 tcgctttcga cctaaaaata aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg
    23941 aaaaatgaat aatacaaata taatcatgtt caacagcagt gtaggtccta aaaaccttgt
    24001 aataaaatac gtgttattta ctactgctaa tttaaaaatc aactggacca ccatcgtaaa
    24061 caattcataa cccgtgacaa agatgatcat actgaaaaat tcagtaaaga tgttgacatg
    24121 gataacgtct ttcattacat acatcaacca tacaattaaa gggagagcta ctgcatagat
    24181 accgataacg ccaatatagt aagcgtcata gatagccccg agaacaatcg tggtaatcag
    24241 taaataacgt ttttcaaacg ccatactgca acataataat gccaatatca gaaagtgggc
    24301 atttgacata taggtgcctt tcgaccactc ccctagcatt cttgttaaat gaccatctat
    24361 caacatcaaa aagaacaaaa cgattggcag atagtacttc attgtttctt tcttcaacat
    24421 ggactaatcc tccgctagcc gttggacaat cgtcacaact gacacgtcat acatttctgc
    24481 ataaggtttc acataaactt cccgatccag cccataacta tctggtttcg ttttaataac
    24541 cgtaccgatc ggcaagttag ctggagaatt ccctccaaga ccggatgtct ggacaacatc
    24601 cccttctttg atatccgtat caccagttaa ttgggtcacc actaaggcat ggagcttttc
    24661 atcatagttt ttaagcaaac caaacgcttc gccattagcc gaagataccc gtactggaaa
    24721 atgattggag ctttcattag aggatgacag taattcgatt ttagacgaag ccgtattgac
    24781 ctcgatcact cggccaatca atcctttttg cgacatgact gccatatttg cttcgatacc
    24841 gtctttagaa cccttgtcta cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat
    24901 gacatttgcc gttacttttt cgtaactagt cagcgtctga ttcaactcaa gttctttttt
    24961 caacgcttcg atttctctct cgtaattctt gttttgctgt gaaacgtcat catagctatc
    25021 gattttttct tttagccgct cattttcggc ataagttgaa aaaagattat ggacagaagc
    25081 tactccattt cgtacccacc gtactggggc agagattgtt ttatcaacca ttgcaacact
    25141 gtcattaacg atggattgga agaaattggg ctttccttca ttcgctcgct gcgcagcggt
    25201 cacacttagt atcgtcacca cgatgataac aataaccaag gtaataataa tatttttatt
    25261 aggattgaac ttcttcacaa agacacctcg aattaattag actcttgtgt tcaattttat
    25321 cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta agcttttttt tagcaaataa
    25381 tagattctgt catttttagc ctgaaagaca aattttttta tttgtttatg gatcaatctt
    25441 tgctttgaat aactgcaatc ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa
    25501 attcgttact tgcataagat gtcggatatt ctacttcttt attttccaat acatacttgc
    25561 tgccttttaa tgtaagattc gagacgggag tcaagcagca ataagctaaa tacttcatat
    25621 ctggtatctg tcttactata tgctctccag gcaaataata agacaaataa ttttgacaat
    25681 ctctgataat aagctgatgg gcaaagggtt tgaagcgagg ttccatacct aaccagagat
    25741 tggctaaaag atgatccaat cttccgcccg tagctccaat caaagtgact tccgcttgcg
    25801 ggtaacgttc aaatgttttt aagataccta actgtgtatc tgtatcgtct ttttctgcag
    25861 gggaacgggt atactctttg gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg
    25921 aatcaaaatc acccactgcc agatctactg ggtagcctgc ttgaagcagg tacagcgcac
    25981 cacggtctat cccgacatag gcatcgtatt tggacagcag agactgcggc cagttttgcg
    26041 gattacctcc agcagctaat aaaattttca tttagtctaa tgcctctttt aatcgttcga
    26101 tttgtccgac tggattttcg gctccataga tatatgatcc tgcaacgaag acatccgccc
    26161 ctgcatcttt acagattttt gctgtttctg gaacgattcc gccatctact tcgattgcat
    26221 aagtccaatt gttctctcta cgtagatcag cgagttccgt aattttttct actgtttctt
    26281 caataaagct ttgtccgccg aacccaggat tgacagtcat gactagtact tgatcagcca
    26341 aaggcaaaac atgtttgatc atggagactg gtgttcctgg attgataact actccagctt
    26401 taactcctaa gctttttgtc atttgaagtg cccgatgaat atgaggagtt gcttcgacgt
    26461 ggacagtaat gatatctgct ccagcttttg caaatgctgg aatgtagttt tctggatcaa
    26521 cgatcatcag atggacgtct aacggtaatt tagtaacagg acggattgcc tgaacaatat
    26581 ttggccctaa tgtgatattt gggacaaatt ggccatccat tacatctacg tggatgtaat
    26641 ctgcgcctcc tttttccact aactcaatgt ctctttgtaa attggcaaaa tctgcactca
    26701 aaatggatgg tgctatcttc attcttgttt cctcctattt tcgtttacct tctttttttg
    26761 catacattgg ttttcttttt tcgatttctt gtaagaattg caaataattg tcgtaacgtg
    26821 tctgtgcgat accacctgtt tctactctat gttttacttc gcagccaggt tctttagcat
    26881 gcatgcactc tctaaatttg caatggacag aagcttctac aaattcagga aattgtctcg
    26941 gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct aaatcctggt gtatctgcta
    27001 ctaacccgcc atagatagga atcagttcca catgacgtgt tgtatgccgt cctcttccaa
    27061 gtgcatcaga aatctctcct gttgctaaat tcagttccgg tgatatttga ttcagtagcg
    27121 tcgattttcc tgctccagac tgacccataa agaccgatag ccgttctggg aagtaacgga
    27181 tcagttctgt cgtatcaccc gtatgattgg gtacaatcac cggatagcct attttagtat
    27241 aggtttgctt gatctcttcg attttttctg ggtcgtctga caagtcggtc tttgtcaaaa
    27301 aaataatcgg ttcgatcgac tcgtattcca aggtaactag aaagcggtca agtagattat
    27361 aagaaaatgc aggctctacg atacttgtga caatgattcc aagatcaaca tttgtgactg
    27421 gtgggcgaac taattgattt tttctcggca agatttccag aagatagccg tctgtttgat
    27481 tttcactctc aaagatcacc tgatctccga caagcggcgt gattttccga ttccggaaat
    27541 ttcctcttcc tcttgtttga taggtctctc cgtttgcgta tacatagtaa aaaccgctga
    27601 ttgcttttct gatttgtcct ttgataagtg ccacctccgt ttttgtttat tttaccatac
    27661 gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa gtatatggct tgaaaattga
    27721 tcaaaagaga tagaaacaca aaaaaga
//
LOCUS       DACDIP010000033        27183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDIP010000033 DACDIP010000000
VERSION     DACDIP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.033"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..536
                     /locus_tag="IV257_001603"
     CDS             <1..536
                     /locus_tag="IV257_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814718.1"
                     /translation="TFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYA
                     NKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQ
                     VASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYR
                     FTFSKQKQPRREVQHSH"
     gene            complement(681..1421)
                     /gene="truA"
                     /locus_tag="IV257_001604"
     CDS             complement(681..1421)
                     /gene="truA"
                     /locus_tag="IV257_001604"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8814719.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1612..2247)
                     /locus_tag="IV257_001605"
     CDS             complement(1612..2247)
                     /locus_tag="IV257_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8814720.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2394..3635)
                     /gene="sstT"
                     /locus_tag="IV257_001606"
     CDS             complement(2394..3635)
                     /gene="sstT"
                     /locus_tag="IV257_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8814721.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3833..4831
                     /locus_tag="IV257_001607"
     CDS             3833..4831
                     /locus_tag="IV257_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814722.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4844..6862
                     /locus_tag="IV257_001608"
     CDS             4844..6862
                     /locus_tag="IV257_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8814723.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6922..7638)
                     /locus_tag="IV257_001609"
     CDS             complement(6922..7638)
                     /locus_tag="IV257_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8814724.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7865..8407)
                     /locus_tag="IV257_001610"
     CDS             complement(7865..8407)
                     /locus_tag="IV257_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8814725.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8412..8639)
                     /locus_tag="IV257_001611"
     CDS             complement(8412..8639)
                     /locus_tag="IV257_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8814726.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8718..10598)
                     /locus_tag="IV257_001612"
     CDS             complement(8718..10598)
                     /locus_tag="IV257_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8814727.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10787..11401)
                     /locus_tag="IV257_001613"
     CDS             complement(10787..11401)
                     /locus_tag="IV257_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8814728.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11543..12103
                     /locus_tag="IV257_001614"
     CDS             11543..12103
                     /locus_tag="IV257_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8814729.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(12408..12722)
                     /locus_tag="IV257_001615"
                     /pseudo
     CDS             complement(12408..12722)
                     /locus_tag="IV257_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(12976..14611)
                     /locus_tag="IV257_001616"
                     /pseudo
     CDS             complement(12976..14611)
                     /locus_tag="IV257_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15166..15729)
                     /locus_tag="IV257_001617"
     CDS             complement(15166..15729)
                     /locus_tag="IV257_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8814730.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(15744..16787)
                     /locus_tag="IV257_001618"
     CDS             complement(15744..16787)
                     /locus_tag="IV257_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814731.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(16920..17324)
                     /locus_tag="IV257_001619"
     CDS             complement(16920..17324)
                     /locus_tag="IV257_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8814732.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(17557..18192)
                     /locus_tag="IV257_001620"
     CDS             complement(17557..18192)
                     /locus_tag="IV257_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8814733.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(18458..19510)
                     /locus_tag="IV257_001621"
     CDS             complement(18458..19510)
                     /locus_tag="IV257_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8814734.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(19523..20461)
                     /locus_tag="IV257_001622"
     CDS             complement(19523..20461)
                     /locus_tag="IV257_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8814735.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(20912..21562)
                     /locus_tag="IV257_001623"
     CDS             complement(20912..21562)
                     /locus_tag="IV257_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814736.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(21662..22171)
                     /locus_tag="IV257_001624"
     CDS             complement(21662..22171)
                     /locus_tag="IV257_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8814737.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(22372..23241)
                     /locus_tag="IV257_001625"
     CDS             complement(22372..23241)
                     /locus_tag="IV257_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8814738.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(23261..24382)
                     /locus_tag="IV257_001626"
     CDS             complement(23261..24382)
                     /locus_tag="IV257_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8814739.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(24384..24779)
                     /locus_tag="IV257_001627"
     CDS             complement(24384..24779)
                     /locus_tag="IV257_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8814740.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(24798..25595)
                     /locus_tag="IV257_001628"
     CDS             complement(24798..25595)
                     /locus_tag="IV257_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8814741.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(25597..26385)
                     /locus_tag="IV257_001629"
     CDS             complement(25597..26385)
                     /locus_tag="IV257_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8814742.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(26399..26875)
                     /locus_tag="IV257_001630"
     CDS             complement(26399..26875)
                     /locus_tag="IV257_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8814743.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(26947..>27183)
                     /locus_tag="IV257_001631"
     CDS             complement(26947..>27183)
                     /locus_tag="IV257_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8814744.1"
                     /translation="ISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKF
                     QTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         8581 a         5517 c         4198 g         8887 t
ORIGIN      
        1 ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc catgctaaca
       61 aagattcatt aacacttata gcacctgaag acgctgttgc tatagctgaa aattacaatc
      121 atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat aatgatcaat
      181 atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac aaacttacaa
      241 ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt tatcgttata
      301 aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct aagtttggca
      361 caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat cagtacacaa
      421 aactagcaat tgcttcaaga tatttatcta tcgttacttt gattttattt ttactttatc
      481 gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct cactagattt
      541 ttatttaaga aagacttttt agctgtacac ataaacaggc taaaaagtct ttttttatat
      601 ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa acatgcgttt
      661 catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag accttgtgca
      721 gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc gattgtttct
      781 atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg caccatttta
      841 tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg ttcgatagat
      901 aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga aaaaccaatg
      961 aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat atccaactct
     1021 tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt gttccaaaca
     1081 taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc aggcatttct
     1141 tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc gatcatttct
     1201 gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc atgggtgcca
     1261 gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg atgcaatact
     1321 ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca tcctgaatat
     1381 ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg gctcctctgt
     1441 cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata gctccttgct
     1501 tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga aaaacaaaaa
     1561 acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt tttactgttt
     1621 gagatgcaag atactcaacc aggcaatggg cacataatta aagaaaaaac tgattctttg
     1681 gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc ttgccagtcc
     1741 ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt ggcttacgtc
     1801 catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata aaaaaccact
     1861 gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg aagattcatg
     1921 ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga acgcgatggc
     1981 tgctgtaact agggtagcta acacacgaga tgtactaaca aatgcagcat acaatgcagg
     2041 caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg cttttcttac
     2101 aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta attcaggata
     2161 atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata ataaaaagaa
     2221 aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac ctatgctttt
     2281 aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta cacgaacatc
     2341 tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa cgttcaacgt
     2401 tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct tttacgcgcg
     2461 cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc agtttccatt
     2521 gaatcctgga taacaccgat aataaatcca ataccgacga tttgcatcgc gacatcatta
     2581 gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc cgcaacacct
     2641 gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa aacaaaaggt
     2701 gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt aatcgcggca
     2761 ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc ttttgtcaat
     2821 ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact tcgggtaaag
     2881 aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg attttcttta
     2941 ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat cgtaccgatc
     3001 aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt agcaattgat
     3061 tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg tacgacttgt
     3121 gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg tgctcgaaaa
     3181 gctagtccca acaatacagc ccagaataag acgccaaggt aattcgcatc aataagagat
     3241 tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc caaactttgc
     3301 ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat tgaacttaca
     3361 ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa aataggcttc
     3421 acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa gaaaaaaacc
     3481 agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat tgaaataaat
     3541 gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac aacaatacgt
     3601 tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca caattttaac
     3661 aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa attctaacaa
     3721 tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg ttttttgaga
     3781 tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc ttatgttttt
     3841 gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga gttatggctt
     3901 cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga tttcagcagt
     3961 cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat ttttacaaac
     4021 ttccgttgtt attggtgcaa atttgatccc actttacatg ggttaccaat atcaaaaaat
     4081 cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc taatagccac
     4141 actagtttgc aatatctttt tttacgtaca agctggcagc ttgaatattc gtgattattg
     4201 gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt gtactttgct
     4261 tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa caacaattaa
     4321 atatctacta ttatttttaa caattggatt agttggctgc tctactctat ttaataaaga
     4381 cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat ttttaatggg
     4441 ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc ctcaagtatt
     4501 tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt cactcatcat
     4561 cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct tcgcaatgta
     4621 ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag gtataaaagg
     4681 aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta atttcccttt
     4741 agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag ggaaggcatg
     4801 gctgataaat atcgggcagt tttttatttg atatttatta gcagtgtccc tcctagttat
     4861 ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat acttcgcttt
     4921 tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc tttttgttca
     4981 taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc agattttcgt
     5041 tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa tatttactca
     5101 gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct tcttactatt
     5161 gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta acttactgct
     5221 cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc cttcagattt
     5281 aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct tattaatcgt
     5341 gggcggattg atccttctcc tattattgat tactagtatt attgtacaac gtagactaca
     5401 aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg ctgtattatt
     5461 aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt tcttgctgtt
     5521 taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa aagataacgg
     5581 accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac ctgctggcta
     5641 ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag atgaaatcaa
     5701 ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa gtgaaagctt
     5761 cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc cttacatcaa
     5821 aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg gtggcggaac
     5881 agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt ctgcttcgtt
     5941 agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata ttaccgatct
     6001 attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca gaaaacaagt
     6061 gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata aattaactta
     6121 tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca aagaaacctt
     6181 gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca ctatgcaaaa
     6241 ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag gaactgctgt
     6301 cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact ataccgatac
     6361 agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta cctttgtttt
     6421 ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat atggattaga
     6481 acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac aagcagctac
     6541 gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg agcatgcgaa
     6601 tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc tacctgcatt
     6661 aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg tctttgttac
     6721 agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag aaattctaga
     6781 agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg caacatgggc
     6841 gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata aaagacatgt
     6901 cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat taaagccatt
     6961 tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat tcccctgatt
     7021 ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat agacgtgctt
     7081 tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat agtctgcttg
     7141 acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag ccatcttcca
     7201 ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac tcatttgatt
     7261 cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa atgcctctaa
     7321 ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc tttctgaaga
     7381 ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt cagtcgtttt
     7441 ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag ttgacttgct
     7501 ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt ttgttgattc
     7561 ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac caattgttac
     7621 taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct ttattctatc
     7681 aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt caaatcttct
     7741 aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc ttcttttatt
     7801 ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga agaaacatag
     7861 acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt ccttggagtc
     7921 tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc aattgttgca
     7981 agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag acattttgga
     8041 aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat ttgttttgac
     8101 catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga acgatttcat
     8161 tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga tcagcatatt
     8221 ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat tgtgcttgga
     8281 tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg gcgccagcag
     8341 ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt tcttccacta
     8401 tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc taatttttcc
     8461 acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc tttgattttg
     8521 ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac cgcatgttcg
     8581 attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat ttttttcatc
     8641 gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc cataaaaaaa
     8701 ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa atacaataaa
     8761 cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat cccactagcc
     8821 atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc aatggcaatc
     8881 actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc atgaaccaac
     8941 gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac atccgttcct
     9001 gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt gattccatct
     9061 ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg aaccttttcg
     9121 tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac ataatcagca
     9181 gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg tttcaactta
     9241 gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat ttgagtgatt
     9301 tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg atgtaaatct
     9361 tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt gttgcccgca
     9421 caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt ctgattatct
     9481 ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat cacggcgcgc
     9541 cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct gtccccttca
     9601 tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa tgtaccagtt
     9661 ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc gcctttgact
     9721 aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg cgcaccaatg
     9781 actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa atcacgacta
     9841 atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg agaaaatcga
     9901 tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa ttcgataatt
     9961 ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta gccatctgct
    10021 aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg tgattcacca
    10081 gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc cacaggaata
    10141 gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat ttccacttct
    10201 tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc ggtcaaagcc
    10261 ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc aaacaaaaac
    10321 aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc aagcacagca
    10381 atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata cgcatggatc
    10441 ataataggta tgaccgcaat aatagaagct atggctagag caatgttata gaacaagaca
    10501 aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa ggcggtgata
    10561 tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct tttcctttca
    10621 ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac tatcaagttt
    10681 agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta gcaagaagac
    10741 tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat tgaatgattt
    10801 tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc ttgctaattt
    10861 tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag ccactatctg
    10921 taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc aacttggtac
    10981 cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct ccatctggcg
    11041 aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa gctcctttac
    11101 tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt tgctgtccaa
    11161 aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct gccaactcgt
    11221 cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac aaaaaatatc
    11281 ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc attccttcaa
    11341 cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa gttagtttca
    11401 tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata gacacagtgt
    11461 ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt tgctttgata
    11521 tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa aaacagaaaa
    11581 agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag caatcactat
    11641 acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac attataagga
    11701 tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa atgaaattct
    11761 ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg actatgttta
    11821 cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt cgtttcagaa
    11881 gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg aagacaagaa
    11941 aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag gttgtctatc
    12001 cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat tattagaact
    12061 cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa catactaagc
    12121 ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta gccaaattgt
    12181 ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa gtagtttgta
    12241 gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt cacctcatca
    12301 ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg taaccaattt
    12361 atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta cctcaattca
    12421 ttttcaaggg gaatcagctc atcatctaac attttacgcc atgctcgctt catttcttgt
    12481 gggtatttaa atggtttcgc ttctggatgt ttggctttat atccttcgta atccttactg
    12541 ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg cagattagaa
    12601 gcatgtaatt ttttctcaga aacaaaccac tcattggaaa agacatgaat caaatcatca
    12661 cttacttgtg taaaatagcg acgtttaatg acaaagatat cttcattgct atctagtgta
    12721 ccttcatttc tttgtgctta aattcaaaga taattttctc aataaagatt tttgattcca
    12781 gtaagattcc tttactcctt ttacaacata gcgttgtcct ttgttttgaa acatcttctt
    12841 cccctctttg ccattaaaaa tagcccaatt ctctataaag aagattaagc ttcattatta
    12901 taatataaag tttaaatttg gtttattact tactttttct tattctctag taagctaata
    12961 ttgaaaaaaa tgtaatcaga tcattatttt tatatgttgt aacttcctga taagaaaact
    13021 aattaaaaga aagaggtaac tacataaact aattaaaaaa gctaacttca tagttggagg
    13081 ataaatggaa gaaacttcaa tgattgtttc tccttgtgtt ccttcaatcg acactagtcc
    13141 tattatcaat ctctcttttt tgtccattta cttttgcgac tgttccccaa taatcaaata
    13201 caggaagcgt tattttttgt cctttagatt catgtactgt atagactatt ttcgaatcca
    13261 tcacttgata aggcatagta tgccattcct tgccaattct tacttcatga ttctgcaaat
    13321 gttctttgta ttgtaatgct tccgtgggtg catagtcata aagtcctccg ggaaggagtg
    13381 tcgtgtaacg aataagattt tcttgattca ttaccaagtg atctctctga gtgacaactt
    13441 tatgttcata gtttgctttt gttgcagcag atacagccaa cagtaataaa acaattgccg
    13501 ttatattaac tacttttctt tgcttagctg atgtgctctt catgaaaacg aacgataaaa
    13561 tcaacgagcc aaacagtact tgaaagccta agattcgcca aggaaattga atcatttgaa
    13621 tgggtgtatt ttgtaataaa gaccatggaa aaaatttggt ggataagagc caagagacta
    13681 ccgcactaag gtaaatcact ttttctttgc gattaaatcc tttaaaaaag aagagtggac
    13741 agatcaaaga aataatacca atgattccta ccgtaaaggt tgtcaggtca ttattcatcg
    13801 caccaatcaa actatccgag acatttaatg cttggtgttg taaggtcgtt ttatctggct
    13861 cattaatcga ttgaaccatg atttgtcgga acatcggata ccaaaagtat gctgttaaaa
    13921 gaatcgtcat tcctactgcc tttgacaact ccacaatcaa ccggatgctt tctttttttc
    13981 ttgtttcact tactacaaag ccgcctaaga ctagtaagcc aacggctatt acactcataa
    14041 aagtggaaag aacatgtgta taaatgagta agctcatact gactgctaat ggaattgcac
    14101 atggcttttt ctcataaatc agtgcgtaag cataatacat aactatcggt ataaccgtaa
    14161 gtgcaatcgc ttcagcaata gcagaacgat gataaatatc aatcgtacgg tacccataaa
    14221 atgtatataa acagctaaat ataaaagccg catcgattct ttttaaaaat tttagtccat
    14281 aatgaaacat cagcatgata gtacaaacat tcaatagcca aacatataag atataactaa
    14341 cgattaaatt attacttatc caataaaaaa tgactgccgg aaaaaaggtc aagtagggat
    14401 aaaaataatt cactccgctc ccataatgat taaacgtcgt aaaattgatt ggaccagaaa
    14461 aaattgaaga taaccctttg actctagcaa aatggaaggc aatatccccc gatgtagaaa
    14521 tagtatgaac atttctaaaa taattgattg aataaagtcc agcaataatt gctaggagaa
    14581 tggacaatat tatttttttc tttacttgca ttacttaatt atatactcct ttcatggatt
    14641 gctttatcta gtataactta aattttaaat aaatcgttat ttatttttac tctcacttaa
    14701 aaaagcacta aaattataag agagggaggt aatggtctga acaccaagaa ttgttccaac
    14761 atcgaattgc ttaactaatg agttcaaagc ttaattatgt ggctcttata tttaccgagc
    14821 gtggattgcg atctctttag cgaaacaaac atctttcata aaaaaaacag ccatatccgc
    14881 tttcttggat atgactattt actactactt tactatcttt cattcttctt gtctcactta
    14941 tgataaggcg ctgcgtatca tgagtgaatg atattaggaa aacttcataa aataaatctt
    15001 ttatacattt ccacttctag atggtcattc atgttagaag aatcaaacta tttttttact
    15061 gtcgtaataa tattaaaaca ctagaacaaa tcaattaaat tccataaaat ataaataata
    15121 gacctagcat catctggtat aaaatggatg ctaggtccgt tatatttact caaaattgat
    15181 tctacgaata gatcttctta tcatttctat tttagtagtc atgcgttgtt gttcttctaa
    15241 aggtggtaat ggtataagta gtttagataa atttgtacca tttacatttg gttgcccagt
    15301 tccgtaagaa attttttcta ataacttcca atataatggc gagtctaaga agcagtctac
    15361 gtattctgat aataatttct caactaacct aattcgaatc aagtaagaag caaaaacaga
    15421 ttcctcactt atttccttaa ccaagaaact ttttcccata gttcctcctg tccttgctat
    15481 caaaatgtca ttttcttcca atcttaaatc aactagttta gaattactaa tatcacagta
    15541 aggcacactg gaccaattta cacgtccctc ttgtatatct gtgattctca aaaacttaac
    15601 attgccttgt tttttcgccg aatctgtata gccatattga atcttagtag aaatacttcc
    15661 taaataaacc catagtcgaa catcgctaga tgatttttct tgtcgttctt tatgaacaac
    15721 agtgatcatc ttgagttgtc cgactatttt ttagttgcag caatcttttt gggacgctta
    15781 tttgcatgaa gcaacgttgc taagtctagt aataaaacat tggctaacac aggattggct
    15841 ttctcgcgat acgctgcggc gccactttca aacaatactg ctagacgatc ttctacaaca
    15901 acgatttgtt ccaaatacgg tggaaatttg attcgtttta ggatccggct aggagagtcc
    15961 cgctcggaca ctaagagttc cgaggtataa ggaccataag aacgactggc taagagataa
    16021 tggccataaa aagccacacc ttgaatctta tcagatgttt ttctgctcat tgtcacggtt
    16081 ggttggattt tgtctttgcg gcgaattgct gttttcattt cagctatcgt cggcaataga
    16141 tagaaagtca actgtccttc accttttagt gtgaagttgc cagataccag ttgatcattg
    16201 ttttgagtca aatacgatgc ttgtggaatc tctgagagat cagttgtcag ccaataagcg
    16261 attggctgtc ccgtctcttc agatgtatct gctaagatat cgatcaaacg taaagcggct
    16321 actcgtccag tagcagatcc tgtgattgtc aaccataaaa gttcccgttt tgtatcatat
    16381 actaagcccc ctacatgtgg ccgtttaggt aaaatcagca gtttgatcgg ctgtcctgtt
    16441 tctctatcca gtacatgaat aatagaatga tgctgatggt catagcagta ggcagaaata
    16501 aacacaaaat cttgagaaaa agccaaccct tgcggcgtca tccattgaca aacctcattt
    16561 tcgccggttt ccggcaccac cattcttgtt tctgttaatc caggaatgtt atattgtttt
    16621 gcttcagcct tttcccaagc tgcttgaaca ataggatacc gttttaactt tttttcaata
    16681 tcctgatgta tccgttgctg cttccaatgc ttaaacttta ggacatagta aacaagaagg
    16741 ccaatgacca gcaacactaa aatcattatt gctacccgct gtgtcatttt tttgcctcct
    16801 tgttctattc gatcaatcgt ccatataccg ctattaatta ccgtaactac atgtttcttt
    16861 tactagcgtt tctttatagg atctttcata atctcattta tagtaacatt aagaatccct
    16921 tattctacta tagtcacttc cacatcaata ttgcctcttg ttgctttaga gtatgggcaa
    16981 aatgcgtggg cttcttctgc gattttctct gcttggtcct tctcaagtcc tggcaaagaa
    17041 acttctaaaa cgaccccgat ttggtagccc ccatcttcta attgaaataa agatacccgt
    17101 gctttgactt cagaatcaga ctcaagatta ttttcttcca gcatgtgttg aaccgcgccg
    17161 ttgaagcaag atgcgtatcc agccgcaaaa agttgttccg gatttgtccc ttcactatgg
    17221 attcctgggc ggtcgatctt catgtgcata cttccgtttg gtgcctcaac ttcgccatca
    17281 cggcctccac gattgatcat tgctgtttcg tacatttttt tcattgagat catcctttct
    17341 ttcgtcgttc tatattaagt gtaacgagta ttttttaatc tgacaaaggt aatgcttgca
    17401 ttcggcgaca tcagttagac aacatcgatg atcagtgata gaagcagttt atctatacaa
    17461 aaaaagaagg gttctatgat atttggtgtc cttactcgcc ttgagtaaca acaccaaata
    17521 tcatagaacc acttaatcag cttcacactg ctttttctac tttcgtagtt gaaaaaaact
    17581 taaacaaatc atcggcaaat agttaaagaa aaaacttaat cgttgaaaga agcccaattg
    17641 cttaaagaag gcaaactgac taatcgacgg aatccgtgct aatccgtaaa gacctgcaat
    17701 tagtaaacta ataactgtta aaacaagata aaaatggcta agcgtacctt gaccactttg
    17761 acgataaagc agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga
    17821 acctatatta tggatccaag tcgatagatt acaagagctt tcattcgtat tgatactgaa
    17881 taagccagta aaaatacaat cacctatccc atataaacca actgttgcaa caacaagtat
    17941 tgcaagtgtt tttgatgttc caacaaatag gtgatacaac gcaggcaacg aaagaacaaa
    18001 aaaaagtcca gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc
    18061 accaaacaca ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat
    18121 atacggtgtc gataaatcac tcatcacacc taataataaa aaataaaaac catatttttg
    18181 caaaaaaatc attttcttcc cctacttttt agtgattatt cgatacatcc aacaatcaaa
    18241 ttttttctca ttttaacaca tatttttatt ctagttgtct tttgaacaat caataacata
    18301 aaggtgagtt tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc
    18361 atcaatctcc tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt
    18421 acgcatccat tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt
    18481 cttctcgatc atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa
    18541 aaacagggat gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag
    18601 aagccaacgg accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa
    18661 tagaaaatag agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa
    18721 tcacaggttt tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa
    18781 ttgtcacacc tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag
    18841 ctacgcccat cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg
    18901 ctactgttga aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca
    18961 ttaacagcgg ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg
    19021 ctgttacaaa agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg
    19081 gtgttaacac aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag
    19141 caatcgatgc tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac
    19201 ccgtttcacc caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg
    19261 ctacaaccat cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga
    19321 tcaaaggtgt tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat
    19381 acgcactaaa atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga
    19441 ttcctgttgc tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg
    19501 aagtttccat tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa
    19561 gctcagtcaa gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa
    19621 ttgcgccatt gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca
    19681 ttgaaggata atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga
    19741 cttctgggac atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa
    19801 tcgtcgccac cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta
    19861 agattgtgca tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt
    19921 gaatattgtc agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt
    19981 ttgcagctgc ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata
    20041 atttgacctg accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat
    20101 tttccattgg tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata
    20161 agacctctgt atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca
    20221 tcgctttggt aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa
    20281 gaacgattgg tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgtt
    20341 ctttgatttg ttggacatgt tctgcccagc catcgattaa taatacttcg ttcccacttt
    20401 ggtgaagcat caacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca
    20461 ttgattcttc ctcctttttc tttctattat actcatttga gaatcttttc acaacctcgt
    20521 tttgttattc ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca
    20581 aatcaataaa aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata
    20641 agaaaataga aacattcata gaaaaaatct cactcctctg attataaaag agggggcatt
    20701 aacctcgtgt atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa
    20761 atattcattg tagtacgatg ttgcgcaaca cagacggatg gtattgctta ttatttatta
    20821 aaatcgaaca atacacttat ttaaaaactt ctttcccaga ttttcttcca tttttacagt
    20881 tataactagt attatcatga aaaatagcct tttattgtct atgggaataa taatttaaaa
    20941 ttggtacttt tttattaatt agttcatcgc ccaataatct ctccagtttc ttagcatctc
    21001 ctgactcaac cggaattatc caagcccagt ctaaataatt ttttaaaatc tcatcatatt
    21061 ttgcaaatat ttcattgcca tattccgtcc ttaaaattat attttcagtg ccagacaagt
    21121 gatttatatt gagttgataa aaaagatctc catctaaaaa aatatcatta gaagaagatt
    21181 tccccacagt aatgaccatt ccatcagctc tatgtgttac atacaaataa ttgtatgacc
    21241 tcatttcacc taatgaatca attatttctt gccctttagc tctaatatat tgatctctat
    21301 tacctatctc actcttattt aacaaaagtt ttttcatgtc aaatttaatc ggctgtttta
    21361 tttttgtaaa ttcgcttcta ctactaatcc aatccgagat taatttttca acatgtccca
    21421 gagtaaactc tgatttattc gctaaatact ttttttgttg tattaaagac atctttcccc
    21481 gaagctcaaa attgagccta catcgattct ctatctcttc aaaaagcatc ttatcttcat
    21541 cattcttata ttcatcattc ataaaaccaa tccagctcct cgattaagat aatattttaa
    21601 tctcatacaa aaactagctc tcatcaaaac aaccattgtt ttgatttctc atgaaatttc
    21661 tttatacttt tctccgatta taaattccta accccttgag aaaattacgc atatatttat
    21721 ctccgcaagg cttaaaattt cgttggcctt cttttctcaa aaatgccccg atttcgcctt
    21781 ttgatggata gatttctgcc tcagcaaaaa cgtctaaaat atcatcagtg gtcatagaca
    21841 aggcgatttt taattttttt agcaacacat tgttgatata tttgatttcc ttgctttttt
    21901 gcggcgtgtc tactatcggc ttatcttttt gcggaccacg cgccaagaga atcaggccat
    21961 ttaggaatgc gtcaaaaact tggttgttga tggttttttc atacacatta ttttctgcat
    22021 tatctttgtt cttatcctgt actttggtca gtaccgcctg agcttcttct ttagtcagtg
    22081 gtacaccgcc tagctcaaaa gctttaatca aatccgtatc tttcaaatca atcgcataac
    22141 gcaatcgcaa taacctatca ttgttattca tttgttcctc cgaagtattt agtctttact
    22201 ttcttacgta ctttttgtct tgctaagatt tttaggatca atgataaaaa ccgcatcact
    22261 tatgataagg cgctgggtat catgagtgaa tgatattgct gaatacaaat agtccgacca
    22321 cattaagttg atcggacttc ataagcattt tattgactaa tacttttttg cttaagaatt
    22381 gattctaaca tcttttgcta aagttaagca tcctatagtt cctgcattat ctgctaactt
    22441 aggtgtgaca atgtattctt cgatatttgg cgtatcgaca tagccgttaa ttagttctaa
    22501 aaaacgtctt tgtactttct ttttcagatg ttcttgttgc atgacaccac caccaaaaat
    22561 tataatatct ggtgaaagca tcagggtcgt attacaggca cattgtgcaa tataatccgc
    22621 ctcgatttcc cagaacggat cttctataga aagatcttgt cccttggttc ccgtacgcat
    22681 ctcgatagcg ggtccagatg ccaacccttc gagacaatca tgatgaaaag gacaagtacc
    22741 agagaaagta tctcctgaat gtctcttaac taacgtatgc cccatttctg gatggctaaa
    22801 cccttcaaca aaaatcccat cttgtatagc tcctccacca attccagttc caacagtgta
    22861 ataaacaaca ctggataaac cttttccatg acctgaaaca tactccccgt aacaagcagc
    22921 attcacatcg gttgtccagg caattggaat ttcgaaatgc ttattcaacg ttccaacaaa
    22981 atcaaaattt ttccaggcag tttttggagt agaggtgata tgtccatagg tctttgattc
    23041 acgatggata tcaatcggtc caaatgaccc tactccaatg ctttctaatt gttctttata
    23101 gtgattaaag aaatcaatta ccaaactcat tgtttcattc ggtgtagttg taggaaaact
    23161 cactctttct aaaaccttta aatcatcatt gccaatggca caaacaaatt tagtcccacc
    23221 tgcttctatt gagccataca tttcttactc caccgtcctt ttatctagga attcgttctt
    23281 ccctgtctaa tagtttttcg aaaaactcat gtggttcctt ttggtaccaa taggcaacag
    23341 aagaaatatc gtcttgacgc tcataaagct tgatgtcatc atttcctagg gtttggaaag
    23401 taacacgtaa attttctttg aaactaatgg ggtcgggtaa atgccagcgg tacaacccat
    23461 gcatcggtaa agcatcatcg ccaaaaccat gaactggatt tggattaggt tttccagcac
    23521 taaaataatc ccgggtgtga tctcttttag attgaaaagg atagcccata aatagcgtat
    23581 taaaattttg agcatatgcc ctttctccaa agttcttttt atggaaagcc caagcgccgc
    23641 caaaataatc ttctgctcct gtagatgtta ttgtagggaa atcttcatct ccatctatat
    23701 aaaacttaaa ttctccttcc ccccaccagt atctttccaa tgcgcacaaa gcaaagtagg
    23761 ttcctacata atacccagtg cctttgattc catcaataag ggtgtaatct tttccttttg
    23821 tcgtgatggg ttcacggttc catttagcat gaaaatacat cgcatcatcc ggtacttcag
    23881 tcaatgcata attaaatgta taaaaaatgt gcttaataaa ctcaggatgt tcattcgtta
    23941 aagtaatctt agcttttttt cgaaatggca tctcaaaata gcaattcatc cctgatgttg
    24001 gattcactac gattggtaag gaattcactt cgcatcttgt cccaaaccca ttacaaaaaa
    24061 aatctcctaa tggactttct acagaaggag tggattcatc atcccaataa atacgcaaaa
    24121 ttaaatcacg taagacgaaa ctcccagcga ttgttttgtc tgtcactgtc atccaaaaat
    24181 gacgtaaaat tccagggcct tccatttctg ctaaagtaat tgtctctcca gcatttatcg
    24241 gaacagaagg tgaccctttt ctcccaattc ctagatgact cgctgtcatt gccgctttcc
    24301 ctttttcacc atttttattt tctggtgaaa tagttctact ttcctctccc ttgtggagcc
    24361 atatattatt taaacaattc attttattcg tcctctatcc tttcttcact tttttgatta
    24421 ttcatataga tgatttgttg ttgtgcctcc gcaataattg ctccaatatc taaattatca
    24481 tttcctaact gcaaagcgat tgacattgcc aagggaagat tcattcctgc aattacgtgc
    24541 actctgtcag aaatataggt aaaaaacttt tgatttacac ttcctccagc catgtcagtt
    24601 aaaacaatta tttcgtctgt agtatccact aactctttaa tttgttcatc tatatcatca
    24661 atttcttcga catatgcaca taagctacta atttgattat gcatacctgt taaaaactct
    24721 agtgtctctt taaaaccctc agctagtatt gaatggctag caatgatcaa tcttctcata
    24781 attcaaatcc ttttccttta cgctaatatt ccaaatgcag ccataaccat cgcaaaaaca
    24841 ataacgagta gaatcaaaat ggtcatactg atttttttct tactgagtag ataatatgct
    24901 gcagcagtaa taattactgg aattaatgtt ggtaaaattt tgtccaacat tggttggatt
    24961 ttcatactaa cttcaccaaa agtgaattta agtggcgttg aaacactgat aactgaagca
    25021 attaatgatc caactactgt tagccccaat actgaagcag cttcagttaa ataattaatc
    25081 ttttcaccgt attcagtaac aatcttcatt ccctgcttat aaccaaattc aaagcctctc
    25141 attcggaaaa agaagattac catgattaaa atgaaccata aaaagattcc tacaggatta
    25201 ttttctaaag ccatataagc cgcaattgat cccatgatgg ttgggatcat cgtcatcaaa
    25261 agagagtccc caataccagc aattggaccc atcgtaccaa ctttaaagtc attaatcgca
    25321 tcttttgctt ctaatccttg cttttcctcc attgcaacac aagcacctaa aataatggct
    25381 gccagccaag gctgtgtatt gtaatactga aagtgattat ttaaagcttc gatgtattca
    25441 tcatcatctt tgtataattt tcttaaaata ggtgacaatg cgtaagtcac agatgcacct
    25501 tgctgtgttt gataattaaa ggtacaaact gacatcaacc aacgatagtt cgctttccta
    25561 agatcctttt tcgttacttg atatttttcg ctcattttat tcatcctccc caataaaccc
    25621 aacatcagaa gctcccgcag aagcagtgat ttgtgcatct ttttcagctc tatgatacac
    25681 attaaatgct aaagcgaatc ctatgatcgc cgctccaata ataggtacct tcaaaaaagc
    25741 ggctaagaaa aatccaacaa gtaaataact aatgtatcga cctgtaggca taaagcgtaa
    25801 caacatcgca ataccaacag caggcaacat ttttccggct aatgttagcc catccataaa
    25861 ccaattaggt aaaacatcca aaacgtaatt aacagcagac tgtcctaaag taacggcaat
    25921 aaatactgga attgctgcag ataaaccaaa gaaaattggc cccaaccaca aaacactatt
    25981 cattttctta aattctcgtt tattcgcata ttcctgtgat ttgcgaacaa caaaaccatt
    26041 taagattttg acaattacat ctagttgcac ccctaacata cctacaggta gtcctattgc
    26101 taatccggct tctactccaa gctggctacc aaatgccgtt gcaataattg cgcacgttcc
    26161 atagtccggt acagatgagc caccaaaacc ggcaactcct aatgacatca attgaactgt
    26221 accgccaact atcaaacctg tttttatatc acccatcaaa attcctgtga ataatcccca
    26281 aaaaactgcg taatttacaa agatcatcgg aatcccataa taatcaacat gtttaataaa
    26341 tgctaatata gtcagcataa taatttgaaa aatagtaaaa tgcattatcc ttactccttt
    26401 atcctaattt tgattgaatt tcctgtgcct tatccgccgg aacaaattgt gtaacaattt
    26461 ctacaccaga atccaacaat tttttatacg tgctgatatt ttcatctgat gcataggctc
    26521 tagaagatag ctgtaatgca ttatctttaa caacagtacc gccaacaatt agtcggtcaa
    26581 ttctaatacc gttatctact aaatgaagga ttgtttccgg ctctttcacg atgacaaata
    26641 ctttttcctg atcgtatttc ccctttttaa aattcgcaat cgctgtttct tcagtgataa
    26701 ttgatacagc cattccagca ggtctagcta aacgcatact tgatttttgg atgtcgttat
    26761 ttgcgacaga atcatcgatc accatcactc tgtttgcttt agacattggt gcccattgcg
    26821 tgacaataat cccatgcaat aaacggttat caactctcga cataacaata ctcataaata
    26881 cttcctcctt tatctttcat tttcttacaa gcaattacca tgccaaaaca aaaagaactt
    26941 gataaatcaa gctcttttta cataatcata aacgtattta acctcatgaa tcgggatgtc
    27001 gacactataa taatgttcaa cgacactgaa agattgctta aaatgtgtct gaaatttttg
    27061 aaattcctca ctcgtttcat tgaaataaat ttcatcttcc aaagtatttt gttttactaa
    27121 tcgttcaatt aagcaactaa tgtgaacata taatccgaaa cacgtattat tatcaaatga
    27181 aat
//
LOCUS       DACDIP010000034        27170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDIP010000034 DACDIP010000000
VERSION     DACDIP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.034"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..129
                     /locus_tag="IV257_001632"
     CDS             <1..129
                     /locus_tag="IV257_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP8814745.1"
                     /translation="KDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(878..1491)
                     /locus_tag="IV257_001633"
                     /pseudo
     CDS             complement(878..1491)
                     /locus_tag="IV257_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2712..3416)
                     /locus_tag="IV257_001634"
     CDS             complement(2712..3416)
                     /locus_tag="IV257_001634"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8814746.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            complement(3431..4300)
                     /locus_tag="IV257_001635"
     CDS             complement(3431..4300)
                     /locus_tag="IV257_001635"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814747.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            complement(4408..5424)
                     /locus_tag="IV257_001636"
     CDS             complement(4408..5424)
                     /locus_tag="IV257_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8814748.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            5614..8337
                     /locus_tag="IV257_001637"
     CDS             5614..8337
                     /locus_tag="IV257_001637"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8814749.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            8465..8887
                     /locus_tag="IV257_001638"
     CDS             8465..8887
                     /locus_tag="IV257_001638"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HAP8814750.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            8905..9375
                     /locus_tag="IV257_001639"
     CDS             8905..9375
                     /locus_tag="IV257_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8814751.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            9399..10181
                     /locus_tag="IV257_001640"
     CDS             9399..10181
                     /locus_tag="IV257_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8814752.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            10171..10995
                     /locus_tag="IV257_001641"
     CDS             10171..10995
                     /locus_tag="IV257_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8814753.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            11030..12028
                     /locus_tag="IV257_001642"
     CDS             11030..12028
                     /locus_tag="IV257_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8814754.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            12045..12899
                     /locus_tag="IV257_001643"
     CDS             12045..12899
                     /locus_tag="IV257_001643"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814755.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(13473..14723)
                     /locus_tag="IV257_001644"
                     /pseudo
     CDS             complement(13473..14723)
                     /locus_tag="IV257_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            15240..15323
                     /locus_tag="IV257_001645"
                     /pseudo
     CDS             15240..15323
                     /locus_tag="IV257_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15412..16823
                     /locus_tag="IV257_001646"
     CDS             join(15412..15967,15967..16823)
                     /locus_tag="IV257_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8814756.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNAYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            16898..18181
                     /locus_tag="IV257_001647"
                     /pseudo
     CDS             16898..18181
                     /locus_tag="IV257_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(18397..19209)
                     /locus_tag="IV257_001648"
     CDS             complement(18397..19209)
                     /locus_tag="IV257_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8814757.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(19226..19957)
                     /locus_tag="IV257_001649"
     CDS             complement(19226..19957)
                     /locus_tag="IV257_001649"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8814758.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            complement(19974..20429)
                     /locus_tag="IV257_001650"
     CDS             complement(19974..20429)
                     /locus_tag="IV257_001650"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8814759.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            complement(20422..20832)
                     /locus_tag="IV257_001651"
     CDS             complement(20422..20832)
                     /locus_tag="IV257_001651"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8814760.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            complement(21363..22322)
                     /locus_tag="IV257_001652"
                     /pseudo
     CDS             complement(21363..22322)
                     /locus_tag="IV257_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            22756..22942
                     /locus_tag="IV257_001653"
                     /pseudo
     CDS             22756..22942
                     /locus_tag="IV257_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252699.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(23183..23398)
                     /locus_tag="IV257_001654"
     CDS             complement(23183..23398)
                     /locus_tag="IV257_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8814761.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            23881..26499
                     /locus_tag="IV257_001655"
     CDS             23881..26499
                     /locus_tag="IV257_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8814762.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTREEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEK
                     FSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISR
                     SYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKYENFLRLLIVVTLSD
                     YVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFV
                     KRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     EYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLKHILSS
                     EMKPVEFNRLIQELIKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRV
                     KLNLYMHTALMMERLFLARTEKAEEPNYDMKPDEDEFYYVSRSIFQSMEMKYNFKVNA
                     YELSLLYELLGPYIQK"
     gene            26576..27064
                     /locus_tag="IV257_001656"
     CDS             26576..27064
                     /locus_tag="IV257_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8814763.1"
                     /translation="MFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQEIKMTILDEVHVVNEEED
                     FF"
     gene            27076..>27170
                     /locus_tag="IV257_001657"
     CDS             27076..>27170
                     /locus_tag="IV257_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8814764.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVA"
BASE COUNT         8915 a         4377 c         5235 g         8643 t
ORIGIN      
        1 aaggattacg aaggatataa agccaaacat ccagaagcga aaccatttaa atacccacaa
       61 gaaatgaagc gagcatggcg taaaatgtta gatgatgagt tgattccact tgaaaatgaa
      121 ttgaggtaaa tgagaagagt taagaaaatt gtacgattca aagatagtag taaagtggaa
      181 gtgtagaaaa tagggataga tgtcttttat ttgttcacat tttataacga agtatttaag
      241 acagctattg aataatgttt cctccacctt tttcattcat ttaccccacc ccgttgttat
      301 atgaccgtgt ttcgagagaa accaataaaa agcataggct aactaggggt tcctttttct
      361 tttgattttt tggaatccgc ttaagtgaaa tgtatctgaa atgaaacaga gataaagtga
      421 ttttttgctg taatgacaag gttttagctg tttcagatac catgtaaccc aaaatagttt
      481 attgagaaaa taggagagta tttgatacta actagtagga aaaaatgaga taaaaagtaa
      541 atggaatcat ttcaggtaat ctcaaataaa aaatatacag gcctgtggca ctttacctac
      601 gggactgtat attttttact ctcagcgggc ttgttttgac tccgtgtttg actccgtcgt
      661 attgatttta aatgatattc gatgaccaat taataggtcg tttttgaaat ttttgtctct
      721 atatgactca gtttgataat tattgataga tcgatttggg tctatatcga acaagggaag
      781 attgcaaaag tcgaagctta aaggattgat attaaaggat taagaggtaa aaaggtatcc
      841 ccataatatc ctctgactcc gtttttgacc tcgcttatca tccgttcatg taagaatcaa
      901 ataattcgac tgtttcattt tgccgatttt ttgatacttt aatataaatg tccatgattg
      961 ttttagaaga agaatgtcct agcctatctt gtacatcttt tacttgagca cctgcttcaa
     1021 agagaagggt agcatgagtc tttctgaagc catggacttt gattatcttc atatctggga
     1081 acttccgtgc tactttatcc atgacatggt tagggtagcc tttgtaaagg aagtcccttt
     1141 ttttattccc tttgatagtg ctgattacag gtatatcatc tatattatct atccgaatcc
     1201 ctctagaaag gttcttttat gttacagaac gtttccattt tttagaatac gtaaggttcc
     1261 attatcaatt gaaattactc cctttgattt gtctgttttt gtaggattaa tacatagaga
     1321 gccctcggat agttctacta acgttttgtt gaagcagata gtttgattat cgaaatctat
     1381 gtctctacat gtcagtccga gtatttctcc ttttcgttcc cctgtataag aagctattct
     1441 aaagaatact aaagcacgct catcttttaa cttatgcaaa cattcaaaaa acagatattt
     1501 cacatatatc taaaattcgg agattgaaag aatgaattta tctaagaatt ttcactaatg
     1561 ttgcgaatat aatatcagaa aatttcgaaa taaattttgt taaaaattag aaaaatccga
     1621 atacttagta gtggataaat ttttttcagg aatagtacgt tttattaaaa tgaaaactaa
     1681 tccgacatca acttttttat cgaagcaaac atcaagcggt aaaaagacct ctttagtttg
     1741 aaaagtgttg ctttcatatt gatttaaatt ttggttaagt aggtaaatta agaaatgatc
     1801 agtgtaagta tttcctgatt tagcgcacct tgttcacttt gaaaatacag gtttttgatg
     1861 gacagatttt gcttcaaatg tttaaagaac atgccactgt acttggtaca ggtcggcgcc
     1921 cccatttgga gttgcgtcag atcgagtcgt gacgattcat tttgtacaaa ttagaattta
     1981 aaattgaata catgacgcgc acattttccc agagagccat attctgtaat taatcacagc
     2041 aattagtgat tatgcacaat ctaaaaaact gatttataat aaaaatatta ttcaaataaa
     2101 taataataac tgcccgcatg atgttgacag ttacaatagc acatagatag actggcaaag
     2161 atggttctta atctttcact ttggtcagaa ttcttgggtt atccttccag tgaaagtcag
     2221 cagaaaatcc ttaatgctta caaataattt ttgcgaaata ggttttattg gtaaaggact
     2281 caatcatgaa tatgaattac ttttatctgg taaatttgga aaaattttgc aaagaaataa
     2341 aaattagaca aatttgctac acataatcaa agattaaaaa agtttttaaa atatagttgt
     2401 ttgaatgggg atatgaatta gagaaaattg gttgattcaa cgggtgctat catttttgat
     2461 agcgtcatat gtgaagtaag cacacctaat gaaaaaggtt caggcttgct ttgaagactt
     2521 attaacaact atgtcgacta tccttgactt agggtagagg agtacctcca cacaccttaa
     2581 ttagatgata atgtttggac tgtaggctac ttagcggatt aagataaaga ggaagtagaa
     2641 aaagagcctt gcggaaagac actttatctg ttatccgcga ggctctttat acatttaagc
     2701 atatttaaaa attatttgga aacagtactt tcaaactttg tcttataagc agggtactta
     2761 gatatttcat attcaaacgg tttctgattt ttagcaaatc caatacttgt aaagacaaag
     2821 actggctcat ttttttctaa atcaagataa aggttttcta aatctgttgg cggtgtaact
     2881 tcaattgagc gtttggcata agctaatcta attcccctac tttctaatac tgagaacagg
     2941 gattctttgg caaaatcatg ttcaacaatt tcaggtaaaa cagatagggg aatgcaactt
     3001 tttgcaatac aagttggata gtcatcgatg tatcgaattc tttccaagac aagcactaaa
     3061 gtattctgct caatctccaa acattcacta attgttgaat cagcaggtat gatggtttgt
     3121 tggataatct tagaactgat tttctttcct tgctgaaccg catcttggaa gaatccgata
     3181 aaagagtcga gttcgtggtc aacatatttt tctgaagctg gtttcactag atacccgcga
     3241 ccttcatttt tagaaataat tccttcgtct tctaaagaag ctaaagcttt ccgaattgtt
     3301 ggacgactca ccgaaaattc ctgggccaaa tctctttccg aaggcaataa ttcagatttc
     3361 cgatcagtgg tgatatacac ttttcgaatt tgttctttta ctttgtcaaa aatcatagta
     3421 atccatcctt ttaccaaggt attaacatta tattaccatt tttttgactt aatatcccag
     3481 attttccttc aattaattcc ttcgccgcta attgacccca caaatagcca atatatcggt
     3541 cataaaaagt tggagatgat ccacgctgaa gatacgcaag atttaagtat ttggtttctt
     3601 ttggtaaatt tttggtaata aattcaatga ttttttgttt gttagatata ccttctggta
     3661 aaagcaatat aggcgagtag aactccgact caaatttttt acatattaag tgtaagtatt
     3721 ccttgtcgtc tctctcaacc caataagtat tatttgcaat ggaagcgtac tctggaagag
     3781 caaagcagcc tcggcccatg acttccatta taataatacg atcatgagca ttagcagtag
     3841 aagttaaatc atcgattgat cggattatag tgtttaaggc gctatcgaag ccaagtgtat
     3901 aatcggtatt ggggaaatcg ttatcaatag tccctgggat gacagatact tgtaattctg
     3961 attcattttt caatagttca gctcctttca aggaaccgtt acctccaata acaattaagt
     4021 tgtcgatctc catcttattt aggttttcaa taataatttt ccgactttcg agatctctca
     4081 ttttctcaaa tctggctgtt ccaataagtg ttccagcttt gaatacctca acttctttta
     4141 gagaaactga ggcaatacat tccttaaata ttccggcata accattctta aaaaagacta
     4201 cttccgaatc tggttcgcta tcaattgttt tttttattgc taataaagct gaattcattc
     4261 cgggagagtc gccaccacta cagataactg cgattttcat tttagatcct cctaaaaaag
     4321 gtgactgcga ttctattctc ccattagaga aaatgaagca atcaaatggg caatggagtc
     4381 gcggtcacct ttttcatatt tttatgatta gtattccatt tgcttataat aacggcgagt
     4441 agttaaaggg tggttacgtt cttcttctag aaaagcacta attcgttcaa agacagtagc
     4501 taagacgact ggggaaataa tttttcgcgt gtcttccgaa atgccaggta agtcatagtc
     4561 ctttgtatca accgttacgt gaacatctgt aaacttagga acgaaattct tcacgcgttc
     4621 tgtgagaggt cgagaatcat cttctccttc tgtaatgatg acacttacac ccgcttcaat
     4681 taattcaaga gttccatgga agaagtctgc tgcatgaaca ggacgagtct taatccattg
     4741 catctcttct aaaatacaca tgccatagta atatgcttcc gcaaagctat caccagcacc
     4801 ggttaaaata tgatatgaag tatctttgtg tttttttgca aaagattttg ccattggttc
     4861 cgcttgctct ttagcgtgca ctaaggcttc aggtattttt tttaaatcct caatataggt
     4921 attgtattga ttgatatcga tttcatttct ttgtttcatt aagaagagag caatcagcag
     4981 tccttgaagg taataatttt ctgaagatgt atcatctaaa acgtcgttat agatagaata
     5041 tgttgcagct ttttctaggg gcgttttagg acttcctacc aaagctaatg ttttgacgtt
     5101 catttcatta cagtattcga ctaactcaag tgtttctttt gttgtaccag ataaagatgg
     5161 gataacaacc aaagatttgt tagaaaatgt tttattggga tgaatcataa attcggcagc
     5221 gataacacga tgaaaaggga aagtgctacg agttctcgcg aattcgattg ttgaatcaaa
     5281 aagaatacca actcctccag acccaataaa gtaaatttct tctaagtcag atgcgatgga
     5341 ttctaaaata ttggaaattt gttcatttgt tgcaacggca ccagcttgag tctttaaaaa
     5401 tctttcttga tcaaaattaa acataaaaat ctctcctttg attaccaatg gtaatatttt
     5461 gtttgcataa ataaaatacc acaggtaata aaaagtgtca aatatttttt aggaaaaata
     5521 ccataggtat tgttaaagtg gaaagatgtt tataaagaat attgaatttt atagtgaaaa
     5581 tcatttatta ttaagtaaag gaggtgggtt catttgaaaa cgaaaatttt aaaagaatta
     5641 aatgatctac ttaacagcta tgattttcca caatattttc cagaagaatg taacgcgcaa
     5701 tatattgccc aaaaatttca aataaaaaga aatacggcta gtaagcatct aaacgaacta
     5761 atggaacaga aaaaagttgt gaaaatcaat ggtcgtcctg ttcttttttt tccagtccat
     5821 acaattgaac ggtatatatc acattcatta gatagttttg agttttcttc aattgattcg
     5881 tttagagaaa aaatttcaaa gaaaaaagca gatcattttt ttgatgattt aattggtgca
     5941 aaacaaagtt taaaagaagc ggttcaacaa gggatatcag caatgaaata tccaggtgga
     6001 ttaccaataa tgtattttgg atcgtccgga gttgggaaga gttacttagt ggaaaaacta
     6061 gctgagtatt gccgacaaac tggtattatt catgagaatg ctccgttcat cgagttaaac
     6121 tgcgctcagt attttaataa tccagagtta ttaacaagtc agttgtttgg ccatatgaag
     6181 ggatcgttta ccggcgccga ttctgaccat attggtataa tagaagcagc agacggtgga
     6241 atcgtctttc ttgacgaaat tcatcgttta ccaccagaag gacaagagaa actttttatt
     6301 catatggata aaggcgtttt tcgaagagtt ggagatagtg gtccttggcg tttatcacat
     6361 gttagatata tttttgcgac aacggaacag gaagaaaatt tttttctaga aacttttcga
     6421 cgaagaattc cgattgtttg tactttgcct aattaccaag atagagaaat tagcgaacga
     6481 aaagagattg tcttccattt attagagaaa gagtctactc tgatcaataa agaaatatta
     6541 gtttctgaag ccgcccttag tttcctgatt cgcttagatt tgtatggaaa cattggtgag
     6601 ctggatggac taataaaaca aactattgcg aataaactat ttagtggaag caataaaaaa
     6661 tatatcgaaa ttcatatttc tgatttacct agcaggtact tgctaacaga aaatattgag
     6721 ttaaaaaacc aaattgcgag ccaacggttc tatatcttta gtaatggtaa ctatgttcat
     6781 gattgtgacg agacttcaac tgtaactaca aatttacgag gtttgattca tcgtgtttct
     6841 aaatattatt cagagtgtca tgatgtgaat agaaatagtg aagagtttcg acgttttgtg
     6901 aaggaccaaa tggcagaatt taatgatcgt tttatttacc aaaaaaattc agaagtctta
     6961 gttaagtatt atttaccgga aatccaaaat attgttcaaa aaatctctcc cagtcttttt
     7021 gttcaccttt ctggcaatac ggtccaagct atagctacct atctcgctat tagggatcaa
     7081 tgggggtatg ggaaagagaa tgcttcagcg aatgaaattg aaaagttata tgaaaaccta
     7141 aagtgtaact atttgacgga tttaaatgta ttcttactga tcttcgagaa cgccctggat
     7201 acaacattga ctatttggga taaactgtat atcagtttgt gtgttattag tgagaccgga
     7261 aatgaagcga aagacattaa cgcaattatt ttagcccatg gatatgcgac agctagtagt
     7321 attgctaatg ttgccaatcg tttggttaat cagtttgtca tagattctat tgatatgcct
     7381 ctagatatca gctttagtga agtaattgac agattacttc actatataga gtatcgaaag
     7441 cctaaagatg gactagtgat tttcgttgat atgggaagtt tagctcaaat taagactgaa
     7501 attgaacaag taatcgaagt tccaacaatg attattaata atgttacaac cgaaatggct
     7561 attgaaaccg cacaactgat tcaaagtacg agtgatatcc aaaaggttgt aaaaaaattg
     7621 ccgtattctc agtttgaaaa gcaagttcta tatcccatca aaattagaaa acgtacaatc
     7681 gttgtttcct gcaatactgg tttgggtacc tccataaaaa ttaaagaaat gatggaaaat
     7741 aatttgtcga aagacttggg cattgaattt ctgccatacg aaaatgaaac tcttagagat
     7801 actcagcaat tagagtttct cattaaaaca aaggatatca ttgccattat cggaacagat
     7861 gatccgcgtg tgtcgaacat tccctatatt tctcttgaag aattatttga tggtgaaatt
     7921 ggcaaactct ataaagttct tttgagagtc tatggaaaag agattgctga ggcaattaat
     7981 caaaatattg ttaaaaatag ctcattaaac cgcttgattg acagtcttac tattttagat
     8041 gccgaaaaag ttgtcaatca aattgaggaa agtttaaaag attatcaaat gagggtgggg
     8101 aaaacgttat catcattgtc caaaattaat ctttatgttc atatcagctg tatgattgag
     8161 cgactaattc gtaatcaggg agtaagtagt tatccagcaa tagatcagtt tcttaagcga
     8221 tacgaaactg aggttgcttc tattcaaaag gcacttagta agattgagaa actttataat
     8281 attaagattc ctatggaaga gattgcatat gtgtgcaaca tcattgtaag ttattgataa
     8341 aataaagtga cactattcac agcgatggaa atagtgtcac tttatttttt gtacgccctt
     8401 tcatttaagt ggtgacagta attggcacag tatttgctaa taacaaagcg aaaggagttc
     8461 gggtatgaga caaatattat gtatgagcca cggtcctcta gcaaaaggaa tgattgaaac
     8521 tttgagtatt atcgtaggag atttggataa tattgatttt cgatgtgcct atgtcgatgg
     8581 aaataatgac gtagaaatga tcatcgatga ttatctaaag gaatctatgg gaaatgagac
     8641 gattgttgta accgacattt ttggtggaag cattaataat gagtggctaa gacggttgcc
     8701 agatctttct aaagtctatt tgatttctgg aatgaattta agtcttttag tggatttata
     8761 tttaaaattc caattagttt catctgatca gattgaagaa attattgatc atgcaattca
     8821 agcatcttct ggttcgatta agttttgtaa tcagctagat tatgagcaaa cggttgatga
     8881 cttttaaata aaggagggaa gagaatgatt aaattagtga ggatcgatca tcgtttgata
     8941 cacggtcaag ttgctttaac ttggacgaac gcaatcagtg cggattgcat tttagtagcg
     9001 aacgatgaaa ttttgaatga tccaattcaa aagacaactt tgaaattagc cgcaccacat
     9061 aacgtgaagg ttgttatcaa atcaattcaa gattcgattg atgcaattaa tagtgggaag
     9121 acagataaat acaaactatt tgtagttacc aaaaccattt ccgatgctaa acggttggca
     9181 aatggggttg aacaaattaa atctgtaaat atcggtagtg ttaaggaaac tagtggcagt
     9241 agcgctttga cgcatacaat ttttgtaaca gaagaggaac gagcagaact taaaggaatg
     9301 gtctcagagg gcattgaagt gttcgctcaa caagttccaa cagataaggc aatagattta
     9361 aaaaaatctt tgtaataaaa aaataagggg gtaatttcat ggatagtctc ttagtacaag
     9421 caattttgct tggtctcttg actgcgttca tcgtaattga ctggatgaca ggtacgaata
     9481 acttatcaag accaatcgta tcttccgtct ttgttggttt aattatgggc gatcttaaga
     9541 tggggattat gatgggggca acactggagt tagcatttat cggagcaata acaatcggag
     9601 cggcacgtcc gcctgatacg gtatctggtg ggattttagg gactgcgttt gctattgcaa
     9661 ctggcaaggg agcagaattt gcgttgacct tggcattccc aatcgctgcg ctattcttat
     9721 tagttgataa tctactaaca ttgcttgttc taccaatgat tgctagaaag gcagatagat
     9781 atgctgaaaa gggcgaacta aaaaatgtgt cgaaaatgca tgttttaggt tttatcttag
     9841 taaaaagttt accacgggct ttatttgtcg gattctcatt ttacttagga agtcctgtta
     9901 tggaacgaat cttaaatgct attccaactt ttgtccaaga tgggattaac attgctgcag
     9961 gcattatgcc agcactaggt tttgctattt tattgcaaat gcttcttaag aaagatgtct
    10021 ttgtatattt cgtaattggt tttgcactct ttagctactt acatgtgccg attctgggga
    10081 ttgcaatctt cggcgcatgt ctagcttata ttatagtaaa catggaaacc aaagctgcaa
    10141 tgggttcaag aggggaggag atggttgaag atgacttcta gtgggaaagc tttaactaaa
    10201 gatgatttaa caaaaatttg tattcggtca tgtgcattag aagcatcatg gaattttgag
    10261 cgccaacaac atatgggctt tgcttttgct atgatgccag cgttagaaaa aatttatagt
    10321 ggcaatgaag aagaaaagca aaaagcagct caaagacatc tagagttttt taacacagct
    10381 ccgccattat caacattcat aatgggaatt gtggcttcta tggaagaaga aaatgccaaa
    10441 aatcctgatg actttgatac ttctacaatc acttctatta aaactgcatt gatgggacca
    10501 ttagctggta ttggtgattc aatgttttgg ggtaccttat tagttatcgg tatcggtgta
    10561 ggtgcttcat tagcaaaaga aggcaatatt ttgggtccat ttttattttt actaatcttc
    10621 aatgtcccta attttcttct ccgttacttt ggagttatgc agggatataa ctttggtaca
    10681 aagctattag agaacattga atcctctggc gtcatgaaaa cgttgactca tgctgcctca
    10741 attttgggtc ttatggtaat tgggggcatg tctggaagta tggtatcaat caaagtagct
    10801 ggttctattg gacaaggtga tgaagcaaca aaaattcaaa gtatcattga tggtatttta
    10861 ccaaatctgc tgtcagtatt atgtgtattt ctgatttact ggatgcttaa aaaaggcttt
    10921 aaaacaactt acgtgttatt cggaatcttt atctttggta tcatagcagc cttcttcgga
    10981 tttttgacaa cttaattgaa taatctaaat aattgtaagg agagaactaa tgttaaacta
    11041 taatgcaaaa gaaatgaaaa aaaaaggcaa acggatttac ggtattcgtg aagatgtaga
    11101 aaaaattgca aagcaagctt atgattctgg aattagtaat attttcttta ctgcttctgg
    11161 tggatcggta gccgtaatgc aaccatttga gtatgtattg atgactgaat caagtattcc
    11221 tgtttactct atgactgcag ctgagcttgt cttaatggga cataaacagt tgaatgaaaa
    11281 atctttagta atcttatact caaaatctgg agatacgaaa gaaactatta aggcagctga
    11341 atatttaaaa gaacgaaacg tgccaaccgt tggtattgta agtaaagaag atacaccgtt
    11401 agaggctcta tcaacttatg caatcaatta cctagacggc cgccctcaag aaatgccact
    11461 ttatttctta attatgtcta tcatgaattt gaatggggaa tttcctgaat acactcgttt
    11521 tgctgatgaa ctgaaaaact taccaacggc tctgaattca gttagagaac aggctgatga
    11581 aaaagggaag aagtatgccg aagaatttaa agaagatcct tatcaaattt ggattggtag
    11641 cgggaattta tggggaatca cctactctta ttcaatgtgt gtgcttgagg aatctcaatg
    11701 gttgcgtaca aagtctgtct catcaccaga gtttttccat ggtaccctcg agttagtcga
    11761 aaaaggagtt tgtgtaacac ttttgaaaac tgagggggaa acacggcttt tagatgctcg
    11821 agttgaaaaa tttgcggtcc aatatacaga taaatttaat gtttttgata ctaaggatta
    11881 tgagctacca ggtattagtg acgaatttcg aaagtacttt tgtgctccag ttatgtgggc
    11941 agccctaggt cgcgtcagtg ttcatcttga agatattcgg aaccactctt tggataaacg
    12001 tcgttattac cgagtagttg aatactaaaa gtaggtgatt aattatgaca gcgtatgtga
    12061 ttggatttgg cgataatgtt gttgattact atgagaattt gaatcaaaaa tttccagggg
    12121 gaaatgcagt taattttgct gtgaatgcaa aaaagagtgg tgttaaagca tactatgtcg
    12181 gtagtctatc atctgatcct gatggcgtgt tattacaaca cgccatcagt tctgaaggtg
    12241 ttgatatctc tttctcggaa gttattgacg agcctactga gcaagcacat gtaactatta
    12301 aagatggaga tagggagttt ataggtggcg tgcgtggctc ccgattgaca cctgctctct
    12361 cagcagaact tattgatttg atgaagaaca gttcattagt tcatactagt tgccatagtc
    12421 gaacagaaac aaaaattaaa gaattaaaaa aatatgatgt taaagtttct tttgattttt
    12481 ctgacttaga taaatataga acgcatgaat acttgtcaga tgtttgtccg tcaatttata
    12541 ttgcacagtt ttcagtcgct acagatgatc aagaggaaat taatcgctta attagtgaat
    12601 gcaaggactt tgaggttccg tatcttcttt ttacgcgtgg tagtaagagc cctgtttttg
    12661 ttgacactcg agagaataag acttatgaag gattcattcg caaagttgag tcacctcatg
    12721 atacaatggg cgcaggtgat gcatactttg cagcttttgc aagtaatttt gtcttatcta
    12781 aacgtgaaaa atcaatgata gaacatgtta ttgattgttt tcacaaaggt gcaaaccttg
    12841 catatgaaac attgatgatt gatggctcct ttggtcatgc tacacctatt aacccttagc
    12901 tttgtgcttt gggcttttat tgctggaatt tgtatagtag actcatcata actatatttt
    12961 tgtatgtgaa aaggcaagag gctatgatga aaataacact ggctaattgc atgagggagc
    13021 atatcgttgt taaagcgtca ttgatagtat gaataaatat ctcgataata ttatattgtt
    13081 atttacttaa tttattgatt agtcaagcaa agataatctt ctgttactta acgagactag
    13141 taggtacaag cacttcatgt tatgtacgac ccaaaaatat tgctgttgat tatttgctga
    13201 aattattgcg agcttcaatt tatttttaaa ggacttggta ggctgcgtga tgaaataaga
    13261 cttggaaggc tgtctaaacc aatatatagt agactaaata gtaaaaaaga gcatggtaat
    13321 tgtctctttg tcaataagtc taatggtaat aagacaagtt ggagattgag aatattttta
    13381 ttgtgtaacc tattttgaag cgtacttaac tagccccggc caaaatcaaa ttttttcggg
    13441 gtatatcaag ggtttgcgtc aataacagaa tgttatttat ctatcacgtt ccttggtggc
    13501 gtgtagagtt gatggttacg tagtagcgta tctaccagac gcgtaaattt tcttgcggtt
    13561 aagacgtgtg ctcgtttgtg tttatttttg ggacctcttt acatttgctt tgataaaaag
    13621 ctttatattg aggaagatat ttttttacag agttggaagc ttcaactaag tagtatctaa
    13681 aatagcgact cctttgtttt gtcagaggtg tattttgtga ccgatagtta acagattgtt
    13741 tgcctttcca acataagcct gcgtattttg ccaattttgt ttgatcttca aatcgttcaa
    13801 tttgaccgat ttcagcgatt aatccagcgg cgtgtacctt tccaatacct ggaacactag
    13861 tcaaatattg atattctgga ataacgacaa caaattgctc ggttgcttta tccaagtctt
    13921 tgatgttctt ttcaagcgca cgaatttcac atacaaaaac atttagaacg acattgatgg
    13981 attcttatgc aagactatct aaccgataac tcacggcaat tgcacgttga atcgatttgg
    14041 catttttttc tggatcttta aagcgacatt tcctttttct tttagtaagt cacagaaagc
    14101 ttccaaagga agcacagcca attcatctag cgtgaagtct tctgtcacaa gagtaatgac
    14161 agtggcacta tagagacttg tcgattcatc gcgcatttat cgagtaagcg tattgcattt
    14221 gtacgttaga ttttccagaa agtgttgttt tgtacgagtg aagttgctta atgagtcgat
    14281 agcgagtgcg tgttaaacgc tgtaaagcca cgtacttctc ttctttgata ggagaagttg
    14341 tgaaccgttg aatacgcaat aaatcagcaa ttcttaacgc gttgtttcga tcagtcgtat
    14401 cttcctcaaa catgcgactg aattgtttca tccgaaaagg attttcaatc ctcacaagtg
    14461 cattgagttt ttttagctct gcatcttcat ggaaaaacat cgaaggatga aagctgtaca
    14521 tggacgttta attccatctc aataacgatt tgaccgaagt gattgttttc attgaacaca
    14581 agaatcagtt ctcgtgtcaa tggagccctt tcaatgtcat tagcaacaga attttcagtc
    14641 aatatagata gctgatcgtc gtctgttagg aagcaaacgt ctaatttttc agaactaacg
    14701 tcattaccaa caaataattt catggaataa aaacctattt tcgatataaa ttctagaaaa
    14761 tgcttcgata ctgtgggatt ccacagaaac tcactgtgtg gtcacgacct cgcctaatag
    14821 aatacggtaa taaaagagct aatgagttga tagtaaacac agcgctttgg ataacagact
    14881 tatgaaggta gacaaagcta caggactgat tagcaaattc tcgttgaatc ctttccatac
    14941 atccattgta ctctgagaaa tcacacaata acgaaataaa ttccggaagg aatatgttat
    15001 tcaaaaaaat ttgtgtttta tagatacttt aaaagaaact tattaggcaa aatatatttt
    15061 acgaagactg atgagttgtg caaaatcaaa tctgagtcag aatgaacttc attctagctc
    15121 agatttttgt ttaatgactt cagtcagttt cttgcattac gtgaatgaaa atgggactgg
    15181 tgatgtccaa accctaaaaa gttagaccaa aatctaactt caggggggat ggtattttta
    15241 tgggaaaata tagctttgaa ttcaagtcaa caatcgttca agagtatctt gaaggagaag
    15301 gtggattagg atatttagca aaaatacaag gagtgagatc aaaaaaaggt acacggatgg
    15361 atcaatcaaa agttagacca aaaaatctaa cttttggggg tcgttatttg tatggcaaaa
    15421 tatccttttg aattaaaact aaaaatcgtt aaagaatatc ttagtggcgt gggcgaatat
    15481 cggcatcttg ccaacaaata cggtatttca agcaagtctc aagtaagtga ttgggtgaat
    15541 gcctatcgtg aatacggaga ggaaggtcta ttgaaaaagc atcaaaatca gaaatattct
    15601 gttcaattta agttaaatgc gctagagtta tatcaaacaa gtgagatgtc ctatcgagaa
    15661 gttgccagca ctttagaaat gaacaaccct gcgcttattg ccaattggat gcgtaatttc
    15721 agagaagcag gtattgaggg gctctcaaaa gagaaagggc gaccacttac tttgactaga
    15781 aaaaaagaaa acaaaaaaga aaatacaaca actgaagaac gcgagcgtat caaggcatta
    15841 gaaaagcaag tccgatccct tcaaattgag aatgcctttt taaaagagtt gaggaagctg
    15901 cgaaaacagg aagcccagca aagacgcaag aatcaatcgc acgaatcatc gccagcctcc
    15961 gaggatcatt caaactagtt gagcttcttg aaacattgaa atttcctaaa gcaacctata
    16021 tgtattggca gaaacggttt gacagaccaa acccagacca agaaattgaa gaaaaaatgc
    16081 ttgaaattcg caaggagcat aaagactatg gctgtctgcg actaaaaaaa gaattagaga
    16141 atcaagggat tcatgtcaat aaaaagaaga ttcaacgtct gataaaaaaa ctaggtatcg
    16201 aagtaaagtc ttacactcga aaatctcgac ggtacaattc ttatcatggg aaggttggca
    16261 ctgtcgccaa aaatcgtatt catcgtcggt tttatacaaa tatctgtcac caaaaaatta
    16321 caactgatac cacagagttt aagtattatg aagtagacac cacaggtgtc gttcgtcaga
    16381 aaaagctgta tttagatcct ttcatggatt tctacaatag tgaaattcta tcttatcgga
    16441 tttctgaaaa accaaacgca ctagccataa tggaaggatt agaagaagca atccagatga
    16501 caaatgattg tccatttcgt agaaccttcc attcagatca aggatgggcc tatcaaatga
    16561 atgcttataa gaataaattg aagcagcata aaatcttcca gagtatgtct cgtaaaggga
    16621 attgtttaga taactcacct atggaaaact tctttggttt attaaagcaa gagatttttc
    16681 atggagaagt ctatcggagt ttagatgaat tgaaaacaaa aattgaccaa tatatctatt
    16741 attacaacca taaacgcatc aaaaagaaat taaattggcg aagtccggtt cagtttcgag
    16801 aagctgtaaa aacagctgtc taacagttaa ttttatcgta caaaaaagga gtggatttct
    16861 ccactccata aaagtctaac tttttggggt cgctacaaat gcttatcgag aatttggaga
    16921 agaaggcctc ttgcgtaagc gaaataagcg aagttattct gttcaattca agatagatgc
    16981 gatagagtta taaaaaacgg gtgagctatc ctatcgtgaa gtggcgaatc atttagggat
    17041 aaaccaacct gcgttagttg tcaattggat gaaaattttt gttcaaaagg attgcacggg
    17101 ctctcaaaaa tgaaagggtc ctcctccatt ttgtctaaga aagaataaaa ggaagaaaca
    17161 agcaaactga cgcctaaaga gcataatcgt atcaaagaat tggaaaaata agtgcgtttc
    17221 cttcagattg agaatacctt tttaaaagaa ttgaggaagc ttagaaaaca ggaagtctaa
    17281 caaagacgaa cgaatcaatc gcacgaatca tcgacaacct ccgaggatca ttcaaattag
    17341 ttgagcttct tgaaaccttg aaatttcctg aattaacctt tatgtattgg caaagacgat
    17401 ttgatcgaag aaattcagat caagaaatcg aagaggaaat gcttaaaata agaagtcaat
    17461 ataaagatta tggctattta cgtatgacaa aggaattgtg ggaccgtggg ttccatgtta
    17521 acaagaaaaa agtacaacgt ttgattcgaa agctgagtat tgaagtacga tcatttacga
    17581 gaaaatatcg ccgctataat acttatcgtg gaagaatcgg catgattaca aaaaatcgaa
    17641 ttcatcgacg cttttacaca agcaactgtc atcaaaaggt tacaacagat acgacagaac
    17701 taaaatattt tgaaatggat aaatacggtg ccaatcgtga aaagaagctg tatcttgatc
    17761 catttttaga tatgtacaac agtgaaattt tattttatcg catttctgaa aagccaagtg
    17821 cgttggctat catggaagct ttagaggaag ctatctgcgc aacggaatgt atctatcgtc
    17881 gaacttttca ctccaaaaag gatggaccta ccaaatgaaa gcttatagtc ataagttgaa
    17941 agaacataag atcttccaaa gtatgtcccg taaggagacc tgcttagata attcgatcat
    18001 tgagaacttc cttagtctat taaagcagga aatttattat ggaagagact atcgtatctt
    18061 tgaagaattg aagcaagcca ttgatggcta tattcattac tacaaccatg agcgtatgaa
    18121 aaggaaacta aactggcaga gtctggtgca atttagagta atagcattca aagctgtcta
    18181 aaatcatcaa gaaaagcaaa gaaaaacgga gtgaattttc tccactccga aaacatctat
    18241 cttttttagg tcactacaga atcaattctg attcatcaga cccataaatt atagatccta
    18301 ataatttata ccatgctctt tttactcata tttacattta tctttgggta ttttatggtt
    18361 taagttgatg tctgatctag ttttattctg gattaattaa cctaataatc caatataaac
    18421 tccaacaata ctaaagacga agatagcaag tataatccaa agaaccttta cttttttctt
    18481 taatagaaag taaacaaaga atgttagccc caatgagaga attcctggaa aaatatcatc
    18541 gagcatttgc tgaacattta tttctccacc ttttgattta aatacggcgg gaatagtaaa
    18601 gcgtaccaga gatgaggtca tggctccaat tgacattaaa cctacgatag atgctgctaa
    18661 cgagaaactt tcaaggtaat tgttctcaaa tgctttttca atggcgtttg ctcccatccg
    18721 atatccaata cgtaagcagc catatctgcc aagaaaatgt aacagcgtat aaattgaaaa
    18781 atacaatatt ggacctaaaa tgtttccttg ggcagctaaa gataagccga tgccagcagc
    18841 aataacgcga aatgttcccc agtaaaaact atcaccaata ccagctacag gccccatcaa
    18901 tgcagctttt atcgatgaaa ttgtttcagt ttcaaattga tctgtttttg cattttcttc
    18961 ttcaagtgct aatgacatac ccataataaa ggttgaaata tgaggcgttg tattaaaaaa
    19021 ttccgtattt cggtaaacag cttcataata tttttcatca ttcccttgat atattttgtt
    19081 taaagcaggt aatatagaaa aactatatcc cattccttgc atatcagtgt atgactcgga
    19141 tccctgtaaa gcaaatgaac gccagaaaac cttgtttatt tcttttttag ttaatttaga
    19201 atctcccata attatcactc tccctttaaa tatttacagg attgcgttta gtttggataa
    19261 ctaataaagc aaccataatt gcagcaacag ctacaccaac tacggggact cctaaatatg
    19321 ccgctaaaag gtatccaatg aaaaaataag gaactgattt tttgtcaatc attccttgaa
    19381 ttagcatggc aaatccaaca gcggggagta aaccagaagc tacggtgaaa ccggtcatta
    19441 atttttctgg cataatattt acaaaacttt ctgtagcatt ggcaccaaaa tatactacta
    19501 aaaatgtaag tactgcggaa ggtaagcgaa cgattaatgg aaaaaaatgc cacttctgaa
    19561 aagatgataa tttgcggttt tcaattgctt ttctaccttg tgaaacggta aaggagttaa
    19621 tcgtaaaagc caacatgttt aatccttgtg ccaatacagc gaaaggcatc gccaatgtaa
    19681 cagctacatc agcacctgct ccacttttta ttgctagagc cgttgcaata gtaccagcta
    19741 cacctacatc tggtggtagt gcagcaccaa tcgttacaat gccaataaac attgcttcta
    19801 atgctgctcc tatcaagatg ccttgatgga gatcccctaa gataagtcca acaacaggtc
    19861 caactattat cggtcgattc atcatagatg ccccaaaata tctttcatct attgtcgtta
    19921 aatacccaac tatgccaatt aaaatgcatt caatcattct tattccccac cttctaataa
    19981 atcttcgctt ttccagttgg agtagcctgc ataattaatt tgaagccgga ctcctttaat
    20041 tttgctaagt agtttttgtc ttctgatgtg atagccacat actcgttaac tttctctttt
    20101 ccctcggcgt accgtaaacc accgatatta attgaatcga cttcccctgg gatagcgttt
    20161 acaatttcaa atgcgctttt aggggaacta gtgactatca tcaaattttg atttttcctt
    20221 ttttcaaagg tacttaaaaa atctgctgaa gtaataatat ctagtcccgt tgatacaggt
    20281 tttgctaatg ataaaatcat ttgtttcatt ttgtcattag cagaggcgtc atcaactaca
    20341 ataatgtagt cagcagatac ggacttagtc caagccattg caacttgacc gtggatcagg
    20401 cgatcgtcaa ttcttaatag tttaatcatt atcttctacc tccattaatt ctgaacagtt
    20461 tataatgctc ttttggcttt cagcaataat catggaaatt ttttctctca atttataatc
    20521 ttcaatgttc atacttaatt ccataattaa ggacaaagtc atacctgcaa ttactgtaat
    20581 tttttttgtt aatttttttt cgtaaacata attcatccaa tgattattta cgctaccacc
    20641 aagtaaatca gtgatgacaa ggactttatc agttccatcg agaagaatat tcgtatcttt
    20701 ttcaatatca tagttatcgt ctgtgtatgc agtcattact tttatgaatg gtgcagggcc
    20761 aaggatatat tcgactgtct ctttcattcc tttcgctaaa tttttgtggc ttgcaagaat
    20821 aattgtgtac ataatccacc tccatttatt tataaataat ataccattgg taatctaaat
    20881 gtcaataaat tatttcgtat taagaataaa taaaatacct ttggtaatta gtggggcgaa
    20941 tctaaaaact ttaactaaaa ttctcttttg taaatttttt tgaacgcggc tatcgtggtg
    21001 attttttatt tctgtttgtt taatgaattt gtaaattagg aggtgatttt tgtgatggtg
    21061 taaacaaagc ctataaaaat gaagtaaagc attgtgtttt gtccagatgt caaatctttt
    21121 atgtatcggt tgtatgtata gtatgttaca cccaaaatag ataacccaag tccaaatgga
    21181 gagacagatg ttctttatat tactggcgaa gacatgaagt attcttataa aatatctaaa
    21241 aatgagtata agtttaaaga aaataattta tctatataaa ctattctgct ttgattagca
    21301 agtctttcaa tcggtaaaat attatttgaa tagatgggat atggtttatt attttctaaa
    21361 tgtcaaatta agttagacaa ttgctcctca cttatgtaac tcaaaaatac ttctaacgat
    21421 gttcgataat ttaaagattt ccttggtata tgatttcttt tggaggcaac cgccgccaca
    21481 aatttttggt caacctgatt gaaatccatt gtattaggaa gtccgtcttt acgtaacaat
    21541 ccattagaat gttcatttaa tccccgttgt gacagcgttc caggatctgc aaaataaata
    21601 gaaatatcgt tagcattact catgtttttc caattcgaaa attcttttcc gcaatcaaag
    21661 gtaatggatt tgaacaagtt tcggggaagc ttttgtagcc agtcagttgt cactttttca
    21721 atatctagtg ctttccgtcc ttcgggtttt aaagtaataa tcacttttga caagcgctca
    21781 accagcgtga tgacagcact tttatgatgg acacccacaa tagtgtcgcc ttccaaatgt
    21841 ccaaattcct gttgaaattc aggatagtcc gtgagacgtt ctgaaatatt ccgtttgaag
    21901 gcttgtttcc ctcgacgttc tttatgcccg ttgggtttcc gttttccttt catgggtagt
    21961 ttcgagcaat caaattagtt tgttttaaac ttgcgataaa gggttcgaac agaacatgaa
    22021 attgaaaact cagcacgacc gataatcaca tcgggtgtcc atccttgcgc caccttgttt
    22081 cggatatacg catcttgttc tgctggcaaa caaatgggtc gtcggccgca ccgtgtttta
    22141 tttttttgat attgttggta atagtcatgg atagattggc cttgtttcag gaagttgtac
    22201 acattgtaga tggtctgtct ggaacgattt gaggtcttag cgactacggt aacagattga
    22261 tgcatattga aataggcttc tatcatcacg agttcatttg ttgtaagatg ggtataggtc
    22321 atttgtcttc actccttata attctttggt cggaactatt ttgagtgtag cacaaatgac
    22381 ttttttagtt gtctagctta attttacaat ctgcgtttaa taaactctca gtgggatagt
    22441 ttgactccgt atatgactcc ggttgatttg attgaattca gatgattata catactataa
    22501 atttctctat tatgttgcca tttgatttga ataatgctga aagaaggatt ttagtctata
    22561 acgaacaagg gaataaaacc aataaaaaaa gatatagaat atcaatataa caaaaagaga
    22621 ttgtgacata attctctgaa aaaagctcta gaaatgacga gtcattcgtc atttctggag
    22681 ctttttaggt aaaataaaag agtacgagag gatactcgca ctctaaatca ttcaaccctt
    22741 gaaaggatca aaatcatgca tacaaattat aacatgaatc aactcacttt agatatcaca
    22801 acttcttatt ccaaaagaag atagcaccgc tgggtttgtc aatgaactcg ttgaaaccct
    22861 tgaaatcaac gagccgtatc tttttggccg ccctaggaat tgtgatttga gtgccatgtt
    22921 aaaattggtt cttttcgcat attgaaaatg attgaagagg actatcggta ttacaaaaga
    22981 caatataacc agcaggtgaa aaaatggaga aggtaattcc gaaaaaagct tcttttggcg
    23041 attgggaata attgttggta atggaggaat aaaatgaatg tttatgcaga cgtttgatgt
    23101 ctacatcaac atccattctt aatactcgaa ggttcgttgt tgaaaaaact aacttttctt
    23161 caacagagga tttacgtgca aatcaagccg agtatttctt caataatctt tgtttgacga
    23221 aactgtataa ggcagtaaag aaagcaagtc ctaatgttaa gtagcccaac ggaacacaaa
    23281 agaacgctgg ttcaatcagc attccgcttg aatcgaggta agaaccgact aggatggaag
    23341 ttgaaaataa caaaatactc aagaaaagaa atatacaagg aataatgaat tttttcatgg
    23401 tgtctccttc tttgtttttc tagattatat agaatagaac ctaggaaaag ataagtgtga
    23461 caaaattgat aggtgtgaaa ggcattgaga tatcaaccag ctttatcctt tggagtatgc
    23521 tattgtgatt aatgagacag aggatgtggg accagacgat actgaatcaa cggaaattgt
    23581 tctaaaccac catcaaatag tagtttttct agtatttgaa ggtgattttt ttatgttcga
    23641 aagtaactgt aaaaaagata aataatcggc agttattctt tcacataagt aggttggatg
    23701 caggtgagag aagtttattg tatgagagga agattggtaa tctaactata gttttataac
    23761 taatatcagt ttaagctcga ttatttttgt ttcaaagtaa aattggaaca gaaatataga
    23821 atagacatat tgtagccgat agtttattat gcttctagac acgttgaact ggaggggaaa
    23881 atgaaaaaga gcgctaaaga tcgagttttt gatgagctga tacgactaac agggaatatg
    23941 tcttctgtca ctgccgagaa aatttcgaag aatctaggta tctctcgtca gaatgccagc
    24001 cattatttga cgagattagt ggaagataaa aaggtcgaga aattaccggg aaagcctgtt
    24061 ttgtggaagc ctttagatga atatgcggtt gtagataata cggagcaaat aaacgaagcc
    24121 tttcattctg tggtgggtca tgacggaagt ctgagagagg tcattcaaaa atgtattgca
    24181 gctgtcaaat atccgcctaa cggactcagt gtattaataa atggagccac gggggttgga
    24241 aaaagttttt tagccacaaa aatttttgaa tacgctatcc acgagcaaat tattgaagaa
    24301 gatgcaccat ttgctatttt aaattgtgcc gattatgcgg acaatcccga actgctttcg
    24361 gcaacactgt ttggatacaa gaaaggggcc tttacggggg ctgagaagga tacagagggg
    24421 ttattggcaa cagccaacaa tggctatctt tttcttgatg aggtccatcg tttgtcgaaa
    24481 gaaaatcaag aaaagctctt tgtttttatt gatactggaa attatcgtcc agtcggtgaa
    24541 aatgtaaact ggcattctgc taaggttcgt tttatcttcg ccacgacgga aaaaagtgaa
    24601 gattatttat tagacacctt tgatcgacgt attcaaatca gtgttatgtt gccaactttt
    24661 gaagatcgtc ccattcgaga acggttagag ctgattcaat tattttttca aaatgaagcg
    24721 gatattttac aaaaagatat tattgtttca aaagaagctt tgtctctaat gttatctcat
    24781 ccctttagtg ggaatattgg taagctgaag aatatcatta aaatcagttg tgcggatgtg
    24841 tacagtcgaa cacgggaaga gccattaacg attgggaaaa atgagatctt gattcagtta
    24901 aatatgctgg aatccgaagt agaaagcttg ccgattgcta gtttattgat aaagaaaaat
    24961 caaaagataa aaacggatcg aatcaaaccg tttgattatg attcgatttt ggaaaaaatt
    25021 actcagctga taaatgaagg taatttcaat gaaaagtttt cttcgataaa gaatgaagta
    25081 cagcatttgg tgcatcacat tgataaggat tcttttttat caatgagtag aaaattgatg
    25141 attcagatgt ttagaaaggt ctgggtatcc gttttaggaa aaacgtacgg attgacgacg
    25201 agtggacctg ttgcagaaat tattagcaga tcctatgtca tttccccaag ctataagaca
    25261 gacttaaccg ggataaatcg tacattagct gcaaatctac ctcggacgac ttacttatgt
    25321 aatcagttct tggagcattt accaccgctc tcagaaaagt acgagaactt tctaagactg
    25381 cttattgtcg tgactttatc tgattatgta gatgaatcca tcgagctgaa gggcctgttg
    25441 cttgctcacg gagatagtac ggctagcagc attcaagcag tagtgaatca attatgcggc
    25501 aattatgttt ttgaagcgtt ggatatgcca gtagagagta atgtgtcaga gattgtcgaa
    25561 tcgacaagga atttcgtgaa acggcaagtg aatacagata gtctagtgct gatcgtagat
    25621 atgggctctt taaatcaaat ctacacacag atcaagaacg atttaaaagg agaattactt
    25681 cttctgaata atttgacgac ctccattgca cttgatattg gcctgaagat gacgatgaat
    25741 gcgccattta aacagattgc ggaggatgca gcagacaagt attcaatcaa tgttcaatac
    25801 tttgaaggct tttctcaaag tagcaacatc atcatttcct gcatgtcggg attgggtatc
    25861 tcggataaac tgaaggatgt ttttcgtcat catttatcgg atgagtccat cgaagttttc
    25921 acgaaggaat acagagaact acgtgaatta atcgaccaaa atgatgaaac ttattttgac
    25981 aagacaaaat tggtcatcac gacttcggat cttccacgtt ccttttcaat tcctaatatc
    26041 aatgtatatg atattttaga tggtgatgga gcagccaatt tgaagcatat tttgagttct
    26101 gagatgaagc cggtagaatt taaccgtttg attcaggagc tgataaaatt cttttcattg
    26161 gaaggaatcg cagaccgttt gaactttctg aacccaacaa tcgtgatgag tgaggtggaa
    26221 acagtcattt ctaaatatga aaattactat catttcactt taaatggccg cgtaaaacta
    26281 aatctttata tgcatacggc attgatgatg gaacgtttat tcttggcgcg gacagaaaaa
    26341 gcagaagaac caaattatga tatgaaacca gatgaagatg aattttatta tgtttcgaga
    26401 agtatttttc aatcaatgga gatgaaatat aattttaaag tcaatgctta tgagctttcg
    26461 ctgctctatg aattattagg accgtatatt caaaaataaa gtgtctaaaa aagtgtctaa
    26521 tctgattttt tcaggttaga cacttttttt tatgtgaatt aacgtgatta aaccaatgtt
    26581 tttaaaagtt ggcacaggaa ttgcttatat gaaagtgaac agaaagaaag gaattaacca
    26641 tatgaagaaa attctaatag cgactcacgg tcacttagct tcaggctttc ttagcagtat
    26701 tcaattactg acggggaagg ttcaagagat tacggtgatt aatgcttatg tggatgagtg
    26761 tgattatgaa caggagttga atgtatttgt aaaccaggtg aacgagcagg atcagatttt
    26821 tgtttttacc gatttatttg gcggaagtgt caatcagaag attaccaaaa cctttttgga
    26881 gaaagagatt gctgtaacta ttttgtctgg ctttaatctg ccaattctgc tcgaaatagt
    26941 attggcaacc aaggaattaa caaaggagga tattcaggaa ctggtaaata aatgccaaca
    27001 ggaaataaag atgaccattc ttgatgaggt acatgtagta aatgaagagg aagatttttt
    27061 ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg aacgattaat tcatggacaa
    27121 gtagcgatga cttggacaaa gagtctaaat ttaaccgggt tagtagtagc
//
LOCUS       DACDIP010000035        26875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDIP010000035 DACDIP010000000
VERSION     DACDIP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.035"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1089)
                     /locus_tag="IV257_001658"
     CDS             complement(<1..1089)
                     /locus_tag="IV257_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8814765.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKEL"
     gene            1299..2300
                     /gene="ccpA"
                     /locus_tag="IV257_001659"
     CDS             1299..2300
                     /gene="ccpA"
                     /locus_tag="IV257_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8814766.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2637..4982)
                     /locus_tag="IV257_001660"
     CDS             complement(2637..4982)
                     /locus_tag="IV257_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8814767.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5261..6163
                     /locus_tag="IV257_001661"
     CDS             5261..6163
                     /locus_tag="IV257_001661"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8814768.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            6376..8151
                     /locus_tag="IV257_001662"
     CDS             6376..8151
                     /locus_tag="IV257_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8814769.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8217..9803)
                     /locus_tag="IV257_001663"
     CDS             complement(8217..9803)
                     /locus_tag="IV257_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8814770.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            10054..10278
                     /locus_tag="IV257_001664"
     CDS             10054..10278
                     /locus_tag="IV257_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8814771.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10420..12171
                     /locus_tag="IV257_001665"
     CDS             10420..12171
                     /locus_tag="IV257_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8814772.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12171..13955
                     /locus_tag="IV257_001666"
     CDS             12171..13955
                     /locus_tag="IV257_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814773.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14281..15132
                     /locus_tag="IV257_001667"
     CDS             14281..15132
                     /locus_tag="IV257_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8814774.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15186..16937
                     /locus_tag="IV257_001668"
     CDS             15186..16937
                     /locus_tag="IV257_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8814775.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            16941..17111
                     /locus_tag="IV257_001669"
     CDS             16941..17111
                     /locus_tag="IV257_001669"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814776.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(17151..18053)
                     /locus_tag="IV257_001670"
     CDS             complement(17151..18053)
                     /locus_tag="IV257_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8814777.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18284..19687
                     /locus_tag="IV257_001671"
     CDS             18284..19687
                     /locus_tag="IV257_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8814778.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19687..21120
                     /gene="ascB"
                     /locus_tag="IV257_001672"
     CDS             19687..21120
                     /gene="ascB"
                     /locus_tag="IV257_001672"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8814779.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21265..22191
                     /gene="trxB"
                     /locus_tag="IV257_001673"
     CDS             21265..22191
                     /gene="trxB"
                     /locus_tag="IV257_001673"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8814780.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22405..24132
                     /locus_tag="IV257_001674"
     CDS             22405..24132
                     /locus_tag="IV257_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8814781.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24204..24497
                     /locus_tag="IV257_001675"
     CDS             24204..24497
                     /locus_tag="IV257_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8814782.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(24589..25449)
                     /locus_tag="IV257_001676"
     CDS             complement(24589..25449)
                     /locus_tag="IV257_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8814783.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25541..25969
                     /locus_tag="IV257_001677"
     CDS             25541..25969
                     /locus_tag="IV257_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8814784.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26131..26307
                     /locus_tag="IV257_001678"
     CDS             26131..26307
                     /locus_tag="IV257_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8814785.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26334..26780
                     /locus_tag="IV257_001679"
     CDS             26334..26780
                     /locus_tag="IV257_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8814786.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8554 a         4787 c         5681 g         7853 t
ORIGIN      
        1 taattctttt ggtgtagtcg tgaatggcac acagccatcg cttgtcacat atacacagtc
       61 ttcgatacgt acccctactt tttcaggaat atagattcct ggttcaagag agaagcacat
      121 cccttcttcg attactaagt catttccttc tacaagggaa gggaattcgt ggacggttgt
      181 tccgatcccg tgacctaaac ggtgattgaa atattcgccg tagccatagc tgttgatcac
      241 gtttcgtgcg atttgatcaa gttcacctgc tgtcacacct ggacggacgg cttcttgtgc
      301 ggcaagctgt gcttcaagag tgatgttgta gattttttct tggaagtcgg ttggtttttg
      361 ataagcaatt gtacgagtag catcactgca atagccattc caaacaacgc caagatcgaa
      421 taacacaagt tcattcggtg aaatcttcgt atttcctggt gtaccatgag ggcttgcagc
      481 atttgctcct gcaaggacta atgtgtcgaa agacatttgt gaaacacctt gttttttcaa
      541 ttggtattca atttctgcaa tgatcgcttg ttcagtcacg ccttctttca ctgctttaaa
      601 gccaatctca aatgcaacat cggcccattt accagcttcg atcagtttgt ctttttcctc
      661 tagagttttg atcagttgta gacgttggat gatggaagtt agatcagtgt aagaatccgt
      721 tcccgtcaag atatcagaga tagcatcgaa gcgctcgaca gtcaaggcgt tcttttcaat
      781 ccctacattg ccaggctgac catagcgagc acgaagttct tttccgatga tctcccaagg
      841 gttttcgctg tctaaatagc cacggacatc atacgcccaa ctactgtttt gagcatcttc
      901 aacttccaaa gcaggagtga aaaggaaagg atcttgttca agagggataa aaagagctag
      961 tacacgctca tgaggatcac tttcgtatcc ggaaaagtaa gcgatgtggc cagggtcgct
     1021 gatataagcc aattcgactt ggtgttgtgc catccattga cgtagttcat tgatttttac
     1081 gttattcatt tggcaaccta ctttctaaat taattttgta ttcaataatt gtaccactga
     1141 tttattaaaa gaccaacgtt ctaaaaactg aaaaaagtaa aaattttcat gaaaacagag
     1201 ataaaacggt tgcaattctt gagattattt gatattctat tttagaaatg taagacgtat
     1261 tttcagaaaa catataaaat atgaatagga gaacacaaat ggaaaagcaa acaatcacta
     1321 tctatgatgt agcgagagag gcaaatgttt cgatggctac tgtttcacgt gtcgtaaatg
     1381 gcaatccaaa cgttaaacca gcaacaagaa aaaaagtctt ggaagttatc gatcgcttgg
     1441 attatcgtcc aaacgcagtt gcgcgtggct tggcaagtaa gaaaacaaca acagttggtg
     1501 taatcatccc tgatgtcagc aatatgttct ttgcttcttt agcacgtggt atcgatgatg
     1561 tggcaacaat gtacaaatac aatattatct tagcaaattc agatggcaac gaccaaaaag
     1621 aagtcaatgt attgaataac ttactggcta agcaagtgga cggcgtgatc ttcatgggtc
     1681 accatatcac agacgaaatc cgtggagaat tttcacgttc aaaaactcct gttgtattag
     1741 ctggctctat tgatccagat gaacaagtag gtagcgtaaa cattgattat gtatccgcaa
     1801 caaaagatgc agtaaacaaa ttggcaaaaa gcggcaataa aaaaatcgct tttgtcagtg
     1861 gtgcgttgat cgatcccatc aatggacaaa atcgtctgaa aggctataaa gaagcattaa
     1921 gcgaaaataa cctaccatat agcgaaggat tgatctttga agcacaatac aacttcaaag
     1981 atggtctatc tctagtcgat cggatccata atagcggtgc aacagctgtc tatgtttcag
     2041 atgatgaatt agccatcggt attttggatg gtttattaga ccgtggcgtg aaagtaccgg
     2101 aagaattcga aatcatcaca agtaacaatt cactactgac agaagtagct cgtcctcgtt
     2161 taacaagtat cacacaacca ttatacgatt taggggctgt atctatgcgc ctattgacga
     2221 aaatgatgaa taaagaagaa gtcgaagaaa aaacgattat cttgccttat ggaatcgaag
     2281 aaaaaggatc tacaaaataa taaaaggaca accattcaga ggttgtgtgc ctgccgttta
     2341 actctgcgtt acaaggagca atttcaaaaa acagcatctc atgtttgatg aatattgtga
     2401 ctcgtgattc ggagttggca ggagaacacc gtttatttgg gaaaaggggt tatgacaaaa
     2461 ttttgttata atcctttttt tgtgtcttaa aaaagagaga aagtggttga aattacattt
     2521 atcgtatata aaacaatccg tgtatagatg ctgcattttg ttcaccacca tcaaaaaaat
     2581 aagggactgt gacaatacta ttgtcacaat cccttattac tgtgtattct attaaatcaa
     2641 tcatctttct catctgattc tttagttgct tgattcgctc ttgcataagc tgaagcggtg
     2701 ttccagtaat ctgtatagtt ttcatcggta ccgccaacgc caaatttaaa ggcgcttttc
     2761 ttcggtccat ttttcgccca tagcgattca actgtttttc ctggtgtttg gatggatttt
     2821 ttattgacag taacttttcc aggtgtttga ccagtaaatc cagatacttt tttcttcatc
     2881 acatcagatg ataattcgaa tttctcatca gtgccaaaaa tgtcaggttt tgcttggtaa
     2941 gcttgattga tcagattggc aagataatta gccgaacgta tccttgcttt gctgtccatt
     3001 ccagtattgt catcgtgccc tgcccagcta ctgatggtaa tagacggagt agagacaatt
     3061 agccatgaat ctcggtattc attggtagaa ccagtttttc cgacccaatc tgcttttcct
     3121 agatttccgt ttaagcttga gatggcatct ttgaatggtg tagtgatttt tgcattgatc
     3181 acactacgca tcatgtcatt catgatcgat gccgttgctt ccgaatagat ccggacagag
     3241 ttgctttcgt gtttataaat tacatttcct gcgttatctg tgatggaatc gatgatatag
     3301 ccttcttcat agacgccgcc gtttgctaaa gcctgaaatc cgttggtctg ttgaagcgtc
     3361 gtgacatcta ctgtacctaa aggagcagat tcaacgcccc aattgtcatt tgccgggtag
     3421 ttcatctttg ccaagtagtg ctcgtaagca tattgtttcg atccaccgga attcaacaca
     3481 tcttgatata agtgataagc cgggatattg ttggaccatt ctagtgactc acggacggtt
     3541 tggaaggtgt tcgatccttc gttagtagca ttgacgattt tctcgccagc attttctcct
     3601 tcttgccaag ttgttgcata atctgataca cgggattctg atccgataag ccccatgtcg
     3661 attgcaggtc cataaacaag aactggtttg attgatgagc cagcttgacg ttctgtatcg
     3721 aaggcatggt tattttgatt ctgactgtaa ttccgtccgc caacgaatcc gtagatgcgg
     3781 ccagttctgt tatccatcaa tacatttccg gtttctactt gactgccagc accatcatct
     3841 aacatgtatc cataattttc aacgccattt tgcaatgcag catagacatc tttatcgatt
     3901 gtcgaatgga ttgtatatcc tttattttgg atcgtttgct gtgccttttg ataataagcg
     3961 tcactgaccg atgaatcaga catatcagcg ttgtcttttt cagccaattg ttgtgcaatg
     4021 attcttgttg cttcattcat cacagtataa tacaagaatt ctctgtcagt ctgttctgcg
     4081 atttgttgtg gtagaaagtc ttttgtaagg tcataggctt ttgcttcttc gtattgttct
     4141 ttcgtaattt ggccttcacg atacatactg aataaaacaa aatcttttct ggcaaggccg
     4201 gcagaaagat cttcttttag cgcgcctgtg ttcgtgtatg gactatacgt gatcgggctt
     4261 tggggtaatc cagcgatata agcagcttgc ggcagcgtca catcttttgc atctacacca
     4321 aagattcctt ttgcagcttc ctggactcca gcaatgtttt gtcccttatt attacgacca
     4381 aatggtgaga cattcaaata cgtagcaatg atctcatctt ttgaaaagta tttttctact
     4441 tgtgcagaaa gaatgatctc gtttgcttta cgtttgaatg ttgtttcatc tgtaaggatc
     4501 tgctgtttga ctaattgctg tgtcaaagtt gatccaccag aagagccaat accagtagct
     4561 tcagatacta atgctcgtaa aacagcttta ggaacgtaac cttggtgttt atcgaagtat
     4621 tcatcctctg tgctgataat tgcagttttc aataaaggtg aaatcttatc agatgaaata
     4681 gtcgtgcgca tcagatctgt ctggattttt gatatctctg tattatctgc ataaacaatc
     4741 tttgacgttt ctgtaatgtt tcctagttct gtctgcagtg cttttttagt cggcaattgt
     4801 gtgtcttcga cgagataggc aaaatagcca gcgcctattc caatacctag agctgcgaac
     4861 atcaaaagaa gaacagtaat aaaaacagtc aatgattgaa aaactctaaa aataatctta
     4921 ggaacaaacc atttttcttt ttttcttcta ttctgttttt ttcgagtatt tttgcgtggc
     4981 aaaagggtca cctcaactaa tggttttatt tcgctaatta tagcaaataa tagaacaaaa
     5041 aaacagacaa ggctgttttt ggcaggaatt tcttttttaa aagagttaaa acgtgagaaa
     5101 atgatcttta ttataaaaga gttcttaagc ttttctttag agaaaatttc agttactatg
     5161 gagaacaggg caaagaaaaa tcaatcattt tctttgtata tcatcttacc aatatatagg
     5221 aattgttagt aacaattgcc tttcaaggag gaatccatcc atgttattag gctcacacgt
     5281 cagcatgagc ggaaagaaaa tgctgttagg agcagcagaa gaagcagcca gttatggatc
     5341 ttcgactttt atgatctata caggcgcccc tcaaaacact agaagaaaag ccatcgaaga
     5401 gatgaatatt gatgccggaa aagcgtacat ggcagaaaaa ggtctaacaa atatcgtcgt
     5461 tcatgcccct tatatcatta atttggggaa tacgatcaaa ccagaaatgt ttccttttgc
     5521 cgtccaattt ctgagagaag agattgttcg tgcggaagct ttaggcgcta ctcaaattac
     5581 acttcaccca ggtgctcatg taggggcagg aacagaagcc ggaattgcta gaattattga
     5641 aggcttgaat gaagttttga caaaagaaca gacggcacaa atcgctttgg agacaatggc
     5701 aggtaaaggt acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca tcgatggagt
     5761 aaaattgaat gaaaaattat ccgttacttt tgacacctgt catacgaatg atgccggtta
     5821 taatattaaa gaagactttt caggtgtaat ggaagagttt gataagatca tcgggttaga
     5881 tcggcttaaa gtactccata tcaatgattc taaaaatccg caaggttcgc ataaagaccg
     5941 gcacgccaat attggttttg gaacaatcgg attcgacacg ctgaataaga tcgtccatga
     6001 tccaatgttt gacaatattt cgaaaatctt ggaaactccc tatgtaggca aagataaaaa
     6061 gaaagcttac gcaccatatg gaaaagaaat cgccatgttc aaagcgcaaa cattcgatcc
     6121 agaagtgttt cctgagttat tagcagaaga agtgatactc taagattttc tatctgacag
     6181 cagcaaaaaa ggaccaggca cattgccttg tccttttttg tttaagaaac aaaggagtac
     6241 tttttgcagt ctttttcatc atgcatcttc tcccccctct ttttaaattt agacaaacaa
     6301 ggtatactgg tagaaaagta aaaaaacgaa aagtttcaca agcaagtggc aaaacgtgat
     6361 tggaaggacg aaaagatgct gaacacttta aaacccttga tcaaccaact aaaaaagaaa
     6421 catacatact ttattcttgg catagcccta ttatttacaa ttattcgttt gtatttatat
     6481 agtaaagcaa tctatggaac agacatgtcg gcaacctatg atgaccagct gctgattcgc
     6541 tatgctcaaa atattttgga cggtaaatgg ctaggggagt atacgactac aacactttcc
     6601 aaaggaatct cctacagctt atttttagta cttgcacata aactgcatct ttcttatgca
     6661 gttttgttcg gcatgttcag tatttttgcc agtatattgg ttgcactcgc gtttaaaccg
     6721 attgtgaaga acaagtatct tcttttaagt atctattttt attttttgtt ttctccgatt
     6781 agttttacac atgaatattc cactcgaaca tatcgtaatg ccctcgtgat tccggctgtt
     6841 gagatcatta tcgcttgttt attagctatt tattaccgca aatacggttc tagcagaaaa
     6901 ttgctgccat ggtttttagg gctttctgtc gcttttcctt tcttttggtt cattcgcgaa
     6961 gattctatct ggcttcttcc ttttactgta gtagctttgg gtatcagttt cgtccagatc
     7021 ttgctagggc aagacttttc tttcaaagaa gtaaagaagt taaaaaaacg actctttctt
     7081 ctctcgaaac gggaatggct gaaactttca ttgtttttac ttcctatagt tggcttttcc
     7141 atcgtctatc aatggatcga atataaaaat gagacaacat acggtattgc aaccatcaat
     7201 gaccgttcca gtggagaatt tggacgtttg atgaaacaac tgatccgaat agatgatgaa
     7261 acgaacttga ataaagaaaa cagtgagata tgggtctcac atgctgcact aaataaagca
     7321 ttagccgcat ctcctacatt ccatactgtt tctacccgta ttgacgaatt atatacgact
     7381 cacgcgtgga ctcaaggtgg aaaagtaaaa gaattacccg gagatatcat cttttgggca
     7441 ttgcgagatg tggtaagcga atctggttat taccaaaaca atgcaaaaga aaccaatgat
     7501 ttttggaaaa aagtcaacca agaattagaa gaagcctatc acaagggaac aatcaaaaag
     7561 aaaaaagagt tctatctgat gggatcagga gatggaaaga cgatgagtga tctgccaatc
     7621 gtattcgatt tccttcaatc aggatacgct tatgcatggc gatataaaga tttccatcaa
     7681 ggaggaaatt atagtttcgg aacgcaagaa caagtcaaac aggctcaaga attattaaac
     7741 cttccgctaa tggagaattg gatcgatagt aatgaaccga gaacacctaa tttccaatta
     7801 acaaaatcag caaaagtcgc gaatgtcatc ataaaaattt accaagttct ggctattccc
     7861 gtgctgattg cttcaatagc agggatatta cttatgatac taggaaccgt atttgcaaaa
     7921 gctgatcgtg cctattatag ttcctctttg attgtggtca ccggattaat actgacacaa
     7981 ttggtttttc ttttcggtgt ctcatggttt tgttcttatg cgccagaaaa aaaggactat
     8041 tttttaagtg tctacactgg tgcaggcgtg ccattaacgc aattcatcgt agtaacaagc
     8101 atgctagttt tcagtaaatt aaatctgaaa aaagctttat cttctaaata aaaagaccct
     8161 tggtagctag atttttaacc taactatcaa gggtctttgt gtggaaaaat cagtttttat
     8221 tcgatttctt ctaccgtttc ctgcaaaagc tgttcatatt ttttgtttcc tttatataaa
     8281 tcaccaatcg tccatgccaa caggagaagg acaagaacaa tgattacata agcgatgtga
     8341 tcagccaatg ctagttgagc ggaagtggct tgctcgccga agaaactttc gatctgtcct
     8401 ggtaatcctc gtaagttcaa gtaagttaga ctaatcacag agaaccagcc aagaagtttt
     8461 acgatgaatg aatttttaaa tcggctaccc atctccgctt ggctgttggt catcatcagt
     8521 aaaggaatca tcgaaaacgg caacgcaaaa gccaagaaca cctgggattc attcattaag
     8581 ttattcaaag cggcatgttc ttcgatcgtt ccttttgtac ttgtaaacaa aacacaaatc
     8641 aatacaggaa tcacagaaag caatcgtgtg accagtcttc ttagccaaat cgggatacgc
     8701 atatggatga atccttccat gatgacttga ccggtcaacg taccagtaat agtagagttc
     8761 tggccagaag caagaagtgc tacagcaaat aaagtggaca agataccaga ttttgctaca
     8821 gcaatcaaaa taccattgct catagctgat ggatcagata aagcttcata cagaccaaaa
     8881 aaggaaggat cttggacaga accggttttg aagacagcga cacccatgat caatagcagt
     8941 gcattgacta gaaaagccat tgacaactgg atattagaat cccaagtaga aaaacgtaca
     9001 gcttgtgcta catcttcttc gttttggtga tcgatcttcc gagtttgtga aactgctgaa
     9061 tgaagatata agttatgcgg catgactgtt gcaccaataa tccctaaagc tccagtcagc
     9121 ggagtttcgc cgccgatgac cggtgagtct gagaatgtat gactgttagg gataaagcca
     9181 gcaaataaac tgtgccaatc tgggttagac aagccaactt gatagacaaa aacgaataaa
     9241 atgacgaaaa ttaagcagac aaccagtgct tcgatcttac gaaacccgac ttttgtcagt
     9301 agtaagagaa cgaaaacatc taatacagta ataaagacgg caatcaccaa tggaatatca
     9361 aaaagtaagt agagggcgat tgctgcacca atgacttctg caatatctgt tgccataatg
     9421 gctaattcag tcaagatcca taaaatgaca cctaatgtct tacttgtgcg tgctcggatt
     9481 gcctgtgcca aatccatttg gctcacgatt cctaattttg ctgccatata ctgtaaaagc
     9541 atagcaatca gactagacat caaaataact gacatcaata aatattggaa attttgtcct
     9601 ccggtgatcg aagtagacca gttacccggg tccatatacc caactgctac taacgcacca
     9661 ggtccagagt aagcaagaag tgttttaaaa aaacttttcc cctttggtac atcaactgtt
     9721 ccatttactt cagctaaaga taaaccattt gtatgttcaa taagtcgatg cttatgaaat
     9781 tgtttattgg attgattatt caagaaaaaa ccacctttcc tatttatcta cgattacatt
     9841 ctacacctca aaaagaaaaa attaaagaaa aagattcggt acgcctaatt ttttttcatg
     9901 gcgcgtcttg ctagatgacg aaagaaattt tgttctcctc taaaaaaacc actatttttt
     9961 gcatgaaact aaagaatacg cctttctttt ttcaataaac tttagtaaaa taagtcattg
    10021 agagcaattc ggaaatagaa aggagagatt tatatgttat caacaggtat tgtcgtactt
    10081 attgctatta ttgctttgct aatcggcgca gtaggtggct ttttcctagc gcgtaaatat
    10141 atgaaagact atttagaaaa aaatcctccc gttaatgagg acatgttgcg atcaatgatg
    10201 atgtcaatgg ggcaaaaacc atctgagaag aaaattcgcc agatgatgca gcaaatgaag
    10261 aaccaaggga aaaaataggt tctgacccaa cgacttttta cgggctgatt ttttcagtcc
    10321 gttttttatt tcgtaatttg ttagactatt cagattattc agcaaaaaag acaagcacag
    10381 acaatcctag gctcttgata aagacaaagg aggagtaaaa tgtcgatttt tagaaaatta
    10441 ggctggtttt ttcgtcagga aaagaaacat tatttaattg gggttttctc gctattcgca
    10501 gtggccttgg tacagttagt tcctccaaaa gtgattggga tcatcattga tgagatcgcc
    10561 gataaaaatt tatcaataaa gatcattttc gggtggatct tcctattgat tatcgctgct
    10621 tttttacaat atctttttcg ttatatctgg cggacgaata tttggggaag tgcagctcga
    10681 ttggagaaag aacttcggca gcagttattc aaacatttta cagaaatgga caatctcttt
    10741 taccaaaaat atcggactgg ggatttgatg gctcacgcaa cgaatgattt gaatgcgatc
    10801 cagaatgtag caggagctgg tatcttgacc tttgcagatt cagtgattac gggcggaaca
    10861 acgatcattg cgatgatttt gtttattgat tggcgcttaa cactgattgc gctgattcct
    10921 ctgcctttgc tggccgtcac ttcacgagtg ctggggtcaa aactccatga tgcttttcgt
    10981 gattcgcaag aagctttttc tagtatcaat gataagacac aagaaagtat cactgggatc
    11041 aaagtcatta aaacatttgg acaagaaaaa gaagatctag cggattttga agaaaagatt
    11101 gaagatgcaa ttattaaaaa caaacgagtc aattttttag actccttatt tgatccattc
    11161 attaccttga ttatcggtct gtcttatgtt gcaacaatta ttttaggtgg aaagtatgtc
    11221 ttggaaggca cgatctcgat cgggcagatg gtctcattta ttagttatat cggtatgctt
    11281 gtttggccga tgtttgcgat tggtcgattg ttcaacgtat tggaaagagg aaatgcttct
    11341 tatgatcgcg tcaatgaact tttgcatgaa aagacgcata tcgtggaaca actgggtgca
    11401 gtgcagaccc cggcaaaagg cgagctgaat gtagctattt cctcttttaa ttatccgaaa
    11461 gaagaatcac cagcattaga acaaatcaag tttgcgatcc aagaaggaga aacattaggt
    11521 attgttggga aaactggtgc gggaaaaacg acgatcatca aattactgat gcgtgaatac
    11581 gatgattatc aagggaaaat ccaatttggc ggacgttcga tcaaagaata ttcattgaat
    11641 gctttattag gatcaatcgg ctatgtgcct caggatcact tcttgttttc gatgaccatt
    11701 cgagataata tccgtttttc taatccaaaa ctttcagaag aagaagtaga gcatgcggct
    11761 tctttagcat ttatcaacaa tgaaatcaag gaattaccaa aaggttatga taccttagtt
    11821 ggagaacgtg gagtctcttt atctggcgga cagaaacaaa ggatttcgat tgctcgagca
    11881 ttgatcaaaa atcctgaatt attaatatta gatgatgcac tctctgcagt agatgccaaa
    11941 acagaagaag ctatcttgca taatttaaaa gaaataagaa aagagaaaac aactatcatt
    12001 gcagctcacc gtttaagtag cgtgatgcat gcaaaagaaa tcattgttgt cgatcaaggg
    12061 aaaatcattg aacgaggaac ccatcatgag ctgcttgcac taggcggctg gtatgaacaa
    12121 atgtgggaga aacagcaatt agaagcgaag atagaaggag gcgaggcctg atggatgaaa
    12181 attatcaatc ggaatggaca aaatcgattc cacttaaaga acagaaaaag atcatcaaac
    12241 gactgatgaa gttcgccaaa ccttttagaa gaacgttcat cgttgctatc ttatttgcgt
    12301 ttgctttgtc tgtgatcaat gtcctattgc caaggatcat ccaaactttt atggatgatc
    12361 atctggctaa acagtcggct acgacacaag ttattttgtt ttttgctggt atgtacttat
    12421 ttggagtcat agtaaaaagt atcatttggt ttttccaatg gtatttgtat tccatggcct
    12481 cattgaagac ctatcagtat attagggtaa aattatttga aaagctgcat acactaggta
    12541 tgcgttattt tgatcagaca ccggcaggat caaccgtttc tcgagtgacg aatgacacag
    12601 aaacgctatt tgaattttgg tacgtctttt tgatggtatt gactgggatc tttgcagtcg
    12661 tttcttcatt ccttgccatg tttcaaatca atggagaaat tgccttgtgg actttggtgt
    12721 ttttgccgat tttaggaatc gtcatctggt attaccagaa attcagttct cgtatttacc
    12781 gaaatatgcg ggaaaaatta agtcagctga acacgaaatt gaatgaatcg atttccggca
    12841 tgcgtattat ccagcagttt cgccaagaag ctcgtttagc taaagaattt gaacaagtga
    12901 acaatgagta tttagacacg cgttatgcga tgatcaaaac gaattcgatt ctgttaagtc
    12961 cgatcatcaa ttttctatat gcattggcga ttgcgttggt gttgactttg tttgggatcg
    13021 atgcgttgca ttctcccgta gaagttggac tgatctatgc atttacgacc tatgtccagg
    13081 gattttttaa tccaatgtct caaatgatgg actttttgag catcttcaca gatgggatgg
    13141 ttgctggtag tcgtatcttg aagattatgg atacggaaga atatgcacca caacaaaacc
    13201 ctggagcaga tcaaatgatt acttcaggta agattgagtt tcgcaatgta agcttttctt
    13261 atgatggaga gcacaatgta ttaaacaata tctcttttgt tgctaatccg ggagagaccg
    13321 ttgcattagt gggacacacc ggaagcggaa aaagctcgat catcaacgta ttgatgcggt
    13381 tttacgaatt ttatgaaggt cagatattga ttgatgatca tgacattcga gaatatccaa
    13441 tggaagagtt acgtaagaaa atggggctgg tcttgcaaga tgctttcttg ttttatgggg
    13501 atatcgcagg aaatattcgg atgatgaatc cagctatcac agacgaacag atacgccatg
    13561 cggctgagtt tgttcaagca aacaaattca tcgatgaatt accaaaagga tatcactcaa
    13621 aagtgatcga acatggtgcc agctattcta gtggacaacg acagctgatc acttttgcgc
    13681 gaacaatcgt gactgatcct aaaattctag tattggatga agcaacagca aatatcgata
    13741 ctgaaacaga aggattgatc caagaaggat tggcaaaaat gcgtcaagga cggacaacga
    13801 ttgcgattgc ccatcggctg tcaacgattc gagatgcaga attgatttta gttttagaca
    13861 aaggtcatat agtagaacgc gggaatcatg aaacacttct agcgcaaaat ggtttgtatg
    13921 cagatatgta caaactgcaa agcagtggta tataaaagat gttgtgacag tagaattttg
    13981 acctaagcac tagagtgaaa ataaaacata gcttctcata tttttcatct gtgagagtta
    14041 gtgtcgtagt caccagattt aaaaaaagat tcgacagcta gtcagaagtg cctaatagcc
    14101 actaagaaag cgttgacaaa agcatttgct ttcgataata taaagaaaac gatacaactt
    14161 ggatagtgat tgcctgatgc aagctaaacc tgatgagtct cccatggttt catggacggc
    14221 tttttgggtt tttctttttg ttttggatac agccctatac atcatggaaa ggagcaaaac
    14281 atgctgattg agaaagtact gaataacaac gtcgtaataa ccagggatga aagaaatcag
    14341 gaaatgatcg tgatgggaaa aggtttggca ttcaaaaaga aagtgggtga cccgattttt
    14401 gactcatgca tcgacaaaaa atttcagttg acaaatgacg ggctttcaca acagttccaa
    14461 gaattggcac aagcgattcc tcttgagtac ttagaaattt cttgtgaaat cattgaatat
    14521 acaaaagagg ctctacaagt ccagttgaat gattctgtgt atattgcatt gacggatcat
    14581 ttgtatacgg ctatcgaacg tcacaaaatg ggaatatttg taccaaatgt attgctgtat
    14641 gatatcaaac aactatttcc taaagagtat gccatcggga aaaaaacagt gaagaaaatc
    14701 tcggataaat accacatcaa gttatcagaa gatgaagccg gatttatcgc attgcattta
    14761 gtcaatgcac aaggcattca aagccagcaa gacaatacct acctgtttac tataacaata
    14821 caggatattc taaatatcgt ccgctattac tataagatta catttgatga agattcagtc
    14881 catttttttc gattcgtcac acacttacgt ttttttctga cacgcgtcct agatggagaa
    14941 aaatcagaag tgggagaagt ttctgaggaa ttgtttcagg ttattcaaaa aaaatataag
    15001 gatacgtttg gttgcgttga gaaaatagca gactttttag ccgagacgtt caactaccag
    15061 atgtccacgg acgaaaaaat gtatttaacg atccatactg cgcgtttagt gcatgagaat
    15121 cgttgtaaat aaaaaaagga ggataaatta acaagaacgt atgaaataag ggtagtgaca
    15181 ttcagttggc taaaccttcc actaataaat tggagggaat agaaatggga aaataccaag
    15241 aattagcgag aaaaatcgta gaaaatgtcg gcgggaagga aaatatcaat ggtttgacac
    15301 attgtatcac aaggcttcgt ttcaaattaa aaaacgaaga aaaagcaaat gatgagatct
    15361 tgaaaaatat ggacggtatt gtaacggtaa tgcgagcggg agggcagtat caagttgtca
    15421 ttgggaatca tgtgccagtc gtgtttgaag aggttataaa agcaggaaac cttacttttg
    15481 atgaggctgt ttcaacagaa aaaatgagac cgtttgatat gcttattgat ggaatatcgg
    15541 gctgtttcca gccattcctt gctatcctag ctgctggcgg gatgattcgt ggactgacag
    15601 cattccttgt cttcttaggc gcgtttgacc gcggttctgg cacatttgtt atgtttgata
    15661 atatcggtga ttctgtattc caatttatgc ctgttattat cggtttaaca gctgcccgta
    15721 aatttaaagt aaatgaattc gtagggatgt tgcttggcgc agcattaatg aatcctagct
    15781 tgtcgctgga agctttgagt ggtgcagcag aagcaccact aacgaccata ttttctggca
    15841 ctatttttga agcacctatt tatcaaacag tattcggcat tccctggatt gcgcgtaact
    15901 acgcttcttc tgtcattccc attatcttta ttgtgttgtt agctagccaa attcagaaac
    15961 caattaaaaa gttagtacct gagatgatcg cgaatttctt cgttccattc ttcaccgttc
    16021 ttattacaat gcctttaggt tttttactcg ttggaccagt attcacattc gcaactgata
    16081 ttttaatggc tggatttgaa acacttttag cactttcgcc agttatttat ggagcaatcg
    16141 taggcttctt ctggcaaatt ctggtgatgt ttggtctcca ttgggcaatc gtaccaatgg
    16201 gattgatgca attttctgtg aatggttggc aaaatattat gacacctgtt gcagtagtgt
    16261 cttttggaca aactgcagca cttaccgcac tctactttaa gcttagaaat ccaaaagaca
    16321 aagcaattgc aataccagcc attgtttctg gtattgtggg gattacagag ccagctattt
    16381 atgggtttac attaccacgt aaaaaaatat ttatttttac ctgtgtcggt ggtgcgttag
    16441 ggggcgcgta tagtgggtta atgaatttaa cttcttggaa tcaaggtgga ctaggcatct
    16501 ttacaattcc caactatatt agaccagatg gtgatttaac agatgtgatt aatgtattga
    16561 ttgggatcgc cattgcgatg gtggtatcct ttacgctgac ttttttcttt tggaaggatg
    16621 aagcaggtga aacagatgat atacagaaaa aatcaggtaa agaaattgta aaaacaccga
    16681 tacaaggaca aatcgctcca ctaaatgcag caaaggatgc agcttttgca caaggaacac
    16741 taggtcgagg aatcttgatc taccctgaaa aaggagaagt acgtgcccct tttgacggaa
    16801 cagtcatgac cctgttcccg acgaaacagg caattggaat ggtttctgaa acagggctgg
    16861 aattgctgat tcatgtcgga ttggatactg tgcagttgga agggaaatat tttgaaagct
    16921 tagtgcagca aggataaaga gtgaaacaag gcgatctgtt gctacgcttc gacaaaaaga
    16981 aaatcgaaaa ggaaggatac agtttagaaa cacctgtgat cgtcaccaat tacacggatt
    17041 atttggatat tttggaaaac caaggggaga cggtcgggcc agacgatgca cttatcaccg
    17101 cactgacctg aacggttagg tgaagaatgg actaggctta actattcttt ttattccact
    17161 tctttatttt caaaattgaa aggctctttc atgatggctg agctgatagg gcgtttatcg
    17221 aatttttgac cgacagaaat tagatggact aaattctgtt gataattttc tgcaaaaaca
    17281 caactatata acacgtctag aagataacaa attgatgtat tgatcgtaaa attaccaatt
    17341 tttgaataca gtcgctcgcg tgtcgtaagt cgcaaagcgg ctgtactcat tgcagatagc
    17401 gttgaatcac cgatactggt caaagctaaa attggaacat gattttgttt caatatttct
    17461 gcggtacgaa ggatcacatt gttttcaccc gtataagaaa tcagaatccc gcacatatca
    17521 ctcgtgcaat tgtacgcttc atagaatgat tcaccaaaag tggtgacaat cgtgacatcc
    17581 attttgatgc gcttcatttt cagcatgaaa tcttgagaaa tcaaagtatt cgagttactg
    17641 gcgaaaatca cgattttttt tgcttgaagc attagttgtt tggctttttg cagttggtca
    17701 tgatgaatga gagaaagtgt atcatgaatc gtcatctgtt ctaaagaagc cattttattt
    17761 gcgatcgtca taataccgtc tgtggtggaa aaaggaagat tcgcatctac gtcttgaaaa
    17821 ttcccttcaa gataggctgt ttcagctaaa aaagcattac gaaaactgac ccagccatca
    17881 aaacccattt tttttgctac acggatgagt gtagaggggt ggacataaca tcgctcagca
    17941 atcgcttgga tggtcatttc gccaagtgct ggagaatcgc ttaagagaaa ctggatcact
    18001 tcattttctg aaggagaaaa ggagatttct ttgatttttt cacgtaataa catgtagata
    18061 ccttcttctt atttttttac gtaaattata acttttttga aaatgatttg taaacggatt
    18121 tatactatat aacttaaaat agacattttt tgacaagata tttacaaaaa tccactagaa
    18181 aacagtaaat tttgtaaatg ttactggaaa gaaacagatt tctattgttt gttttgttta
    18241 caaaaaagat aatgaaagtg taaaaaagta aaggagtgag aacatggcaa aaggaatcaa
    18301 aatcgctacg attggtggag gcagcagtta cacccccgag ttgatggaag gatttatcaa
    18361 aagatatgag gaattaccga tacgagaaat ctggctcgta gatgtagaag aaggaaaaga
    18421 aaagcttgag atcgttagtc agatgtctca gcgtatgtgg gatgcttctc cgtatgaagt
    18481 caagatctat gcaactctgg atcgggaaga agcattgaaa gatgcggatt ttgttacgac
    18541 tcagtttcgt gtcggtcaat tagatgcacg aatcaaagac gagcggatcc ctagttatta
    18601 tgggatgatt gggcaagaaa caaatggtgc tggcgggata ttcaaagctt ttcgtaccgt
    18661 tccagtcgtt ttggcaatcg ttgaggacat gaagcgttta tgtccaaatg cttggctgat
    18721 caatttcgcg aacccaagtg ggatggtgac agaagcagtt atcagatatg gcaaatggga
    18781 taaagtgatt ggattatgca atgtgccagt gatggcgatg atgacagaac cagcaatgct
    18841 tggcaagaaa aaagaagagc tgatctacaa gtttgccggt ctaaatcatt tccattggca
    18901 caaagtagca gataataaag gaaatgaccg cacggcagaa ttgattgatc gtttatacga
    18961 tgaagagaat ggattaccga aaaatatctt tgatatcccg tttttcaaag aacaattaaa
    19021 acaaatgaac atgatccctt gtggctacca ccgctattac tatcgtgaag aagaaatgct
    19081 ggaacatgct ttagaagaat atcaaacgat tggtacaaga gcgcagcaag tgaaagaaac
    19141 agaagcagag ctatttgaat tatacaaaga tccagaatta gaccataaac ctgaacaatt
    19201 gcaaaaacga ggtggcgctt attattcaga cgctgcctgt gaaacgattg cttcaatcta
    19261 tggaaataaa gaaacacaga tcgtggtatc tacaaagaat gatggagctg ttcctgacct
    19321 gccggcagat tgtgtagtag aagtcactgc ctatgttggc gcacaaggag cgagaaatgt
    19381 agcgtttggt tctcttcctg ctgctgaaag aggctggttg caagtaatga agaatatgga
    19441 attgctgacg atcgaagcag ctgtgactgg tgattatggg acagctttac aggcgtttac
    19501 gatcaatcca cttatacgat caggaaagac agcaaaacgg atcatggatg agttgtttat
    19561 tgcacaccag cagtatttac cgcaattcaa ggagacaatc agccgattaa aagaagaagg
    19621 aatcgaagtt caagatgaac tggcaagaga attatcaggt aatacacata taatggagga
    19681 agtataatgg gattcagaaa agatttttta tggggcggtg ctacagcagc caatcaatgt
    19741 gagggcggtt atgatgaagg tggacgcgga ctagcaaacg tagatgttgt gccaatcgga
    19801 aaagaccgtt ttcctgttat caccggaaag aaaaaaatgt ttgaatttga tgaggatcat
    19861 ttttatccag caaaaattgc aatcgatatg tatcatcgtt ataaagaaga tattgctttg
    19921 tttgcagaaa tggggttcaa gacttatcga atgtccatcg cctggagtcg tatttttcca
    19981 atgggggatg agaaagagcc gaatgaagct ggcttgaaat tttatgagga tgcctttaaa
    20041 gaatgccaaa agcacggaat tgagccactt gtgacgatta cgcattttga ttgtccaatg
    20101 catttgatca aagaatatgg tgcgtggcgt tccagagaac tcgttggttt ttatgaaaat
    20161 ctgtgtcgag tcatcttcaa tcgctataaa ggacttgtca aatattggct gacattcaat
    20221 gagatcaata tgatccttca tgcaccattc atgggcgcag ggctgtattt tgaaaaagga
    20281 gaaaatgaag aacaaatcaa atatcaagcg gcacatcatg agttggttgc aagtgccatc
    20341 gcaacgaaga ttgctcatga agtagatccg gagaacaaag taggctgtat gctggcagca
    20401 ggagcatatt atccatacac ttgcaatcct gaagatgttt gggcttctag aaaatcagat
    20461 cgagaaaatt atttcttcat tgatgtccag tcacgaggag aatatcccgc ttatgcattg
    20521 aaagaaatgg agcgaaatgg cattcagatc gaaatggaac caggggatgc cgcattgctg
    20581 aaagaacata ctgttgattt tatctctttt tcttattatg cttcccgagt ttcgactacc
    20641 gacaacgaac tcttggaaca gactgcagga aatatctttg cttctgtcaa aaatccatat
    20701 ctgcaagcaa gtgaatgggg gtggcaaatt gatccattag gcttgagaat aacgatgaat
    20761 gatatttatg atcgttatca aaaaccattg tttattgtag aaaacggctt aggtgcagtc
    20821 gatacgccag atgaaaatgg ttatgtggaa gatgattacc gaatcgatta cctggcagcg
    20881 catatccagg cgatgaagga tgcagtagaa ctagacggag tcgatctatt aggctatacg
    20941 acttggggat gtattgattt agtatcagct ggtaccggtg agatgaagaa aaggtacgga
    21001 ttcatttatg tcgatcgtga taaccaagga aatggcacat tagcccgttc aaagaaaaaa
    21061 tcattcaact ggtataaaaa agtaattgat tcaaatggag aaaatctttc gaacgattga
    21121 tcaatgattt ataaaagtct attgtttttc agcggatttt tcgagtataa taaagctatt
    21181 cgagaaacgc aagcagaaca atagactttt tttcgcattg gctgtaaagt atgcttactt
    21241 gattaagtaa aggagaatct acatatgtat gatgtaatcg ttattggcgc aggtcctgct
    21301 gggatgactg ctgctttata tgcttcacgt tcaaacttat ccgtattgat gatcgaacaa
    21361 ggtgcccctg gcggccaaat gaacaatacg gcagaaatcg aaaattatcc tggattcgat
    21421 tcgatcatgg gccccgatct agcttataag atgtatgaaa gtgtctcaag atttgggaca
    21481 gaaaatgctt atggaatcgt tcaagatatc aaggatcacg gcagccataa agaagtcatc
    21541 tgtgaagata aaagttacga aggaaaaaca gtcattatcg caacaggatg tatccaccgt
    21601 aaattaggcg tacctggcga ggaagaattt gctggacgag gcgtttctta ctgtgcagtc
    21661 tgtgatggtg cgtttttccg taacaaacgt ctagtagtca tcgggggtgg ggactcagca
    21721 gtagaagaag ccatctattt gacacaattt gcttctgaag tagtgatcgt ccaccgtaga
    21781 gatgaattac gtgcacaaaa gatcatccaa gaccgtgcat ttgcaaatga aaaaatttcc
    21841 tttatgtggg atacagtcgt tgacgaaatc gttggaaacg aaatggtagt gacaggtgtg
    21901 aaagcacgta atgtcaaaac cggcaatgaa tttgatttag cagcagacgg agtattcatc
    21961 tatgttggac tggatccatt aactgcacct tttagagaag caggtttgac gaacgaagct
    22021 ggctggattc caacagacaa cgaaatgaaa acgaatatgc caggcgtttt tgccatcgga
    22081 gatgttcgtg aaaaagattt gcgacaaatc actacagctg ttggtgacgg cggtattgct
    22141 ggacaacaag tgtataaata cttggagtct ttagaagaaa caacagcata agtttaaaaa
    22201 gagtagaaga acgggttcaa ttgttctttt actctttttt ttcgcgtgaa atttactgaa
    22261 tctgatttat gcgaacacgt ccaacacctt ttgataaaag aatcctttct tgatttaagc
    22321 gttttttggt cctcatactt tcatatctaa taagtgaatg ctatacttat taagaaccat
    22381 atttcagaaa gaggtgttat tttcatgtct tgggaacaag tgtatgaaca atgggtacat
    22441 gaagaaaatt tagaagaaaa cttaaaaaaa caattaaatg atctgggtga agatcctgaa
    22501 aaacttgaag atgcttttta tgcaccgttg gaattcggta ctgcaggtat gcggggaatc
    22561 ttaggacctg ggatcaatcg aatgaacatc tatacggtaa gacaagcaac agaaggacta
    22621 gcacgtttca tggatacaca aggtgaagaa acaaaacgcc gaggtgtcgc tatcgcttat
    22681 gactcacgcc atcagtcacc agaatttgca atggaagcag ccaaaacact agcacatcac
    22741 aatatcccgt cttatgtatt cgaaagtctg cgtccaacac cagagctttc ttttgcagtt
    22801 cgtcatttga agacgtttgc gggaatcatg gtaaccgcat ctcacaaccc agcggcttac
    22861 aatggatata aagtctacgg agaagatggc ggacaaatgc ctcctgcaga cgcagatgca
    22921 ttgacgaaat atgtccgtga agtcagcaat ccgctaaaag tcgaagtatt atctgacgaa
    22981 gaagctaaac atagcgggtt gatcacgatc atcggagaag aagtggacgc tgcttatcta
    23041 gaagaaatca aagcagtgac gattgatcgt gaattggttg aaacaatggg gaaagatttg
    23101 aaacttgtgt acacgccact tcatggaaca gggaaaatgt tgggcgaacg tgcgttgaaa
    23161 caagctggat tcgaacaatt cgtactagtt cccgaacaag ctgtggcaga tccagatttc
    23221 actacggtca aatcgccaaa tccagaagaa cattcagctt ttgaatatgc tatacgactt
    23281 ggagaaaaag aaggagctga cttgctgatt gcaacagatc ctgatgcaga ccgtttaggc
    23341 gcagcggttc gtttgccaga tggttcttat gaagtattga ctgggaacca aatcggagca
    23401 ttgtttgctc aatatatttt ggaagcacat caatcaagag gcactttacc agaaaacgca
    23461 gcggtattga aatcaatcgt ttccagcgaa ttgcctacag caattgcgga acactacggt
    23521 gctaccatgt tcaatgtgtt gacagggttt aaattcattg ctgaaaaaat ccaacaattt
    23581 gaagaagacc attcacatac ttttatgttt ggattcgaag aaagttatgg ttatttagtt
    23641 aaaccttttg ttcgcgataa agacgcgatc caagcattgc ttttattagc agaagtagca
    23701 gcttactata aaaaacaagg caaaacgttg tatgacggct tgcaaacgat ttttgaaacc
    23761 tatggctatt tcgcagaaaa aacgatttct gtgacaatga gcggacaaga aggttctgcg
    23821 aaaatctctg ctttgatgga aaaattccgt aaagaagcac cgacagaatt tgccggagca
    23881 aaagttattc aaacagaaga ctttaaagaa ttgacgagaa cagaccgtga aggaaacgta
    23941 gaaaaactaa caacaccgcc atccgacgta ttgaagtata cattggaaga aaatggctgg
    24001 attgctatcc gcccttcagg aactgagccg aaaatcaagt tttatattgg tgtaaaagga
    24061 agttctacag aagacgcagc cgaaaaagta gcctcttatg aagcgattat caaaaagatg
    24121 acagaagaat aaaaaacttg agactgatgg aaaaagtggt aaactttaga agtcagtctt
    24181 ttttcaagtt ggaggtgaat ggattgccag acacgcaaaa atcaaaagta gagaaagtca
    24241 gtcaattatt caaaatgttg agtgatccca ctcgtcttaa aattctgcta tatctgaaag
    24301 acggggaaca aaatgtgaca gcaatcaccc aagcagtgga aatggagcag tctgctgtat
    24361 cgcatcaact acgtttatta agagaaaatc atgtcgtcaa gtctcatcgc gagggaaaag
    24421 cgattttgta tagtcttgat gattctcatg tactggatat tttgaaccag acactcaaac
    24481 atgtggaaca tcaataacga taggtgcaaa tagataaaca ctggagtgtg acaacgaaga
    24541 aatttcgttg tcacactcca gtgtttttta agaaaaagac aaaaatgatc aatcgctttt
    24601 ttctgttgtt tcagttccat aataactatg atcttctagg tctaaagcat tcaaaataag
    24661 cgaagccgtt tcttcaatag ataaagatgc tacattgatc actacacatc ctagtttttc
    24721 ataaagatca ttcgcaaaat cgagttcagc tctaattttt tcgatatcgg aataagcagt
    24781 atcagggttt aatccgtaag agcgcatccg ttctttacgg atattattta aaatatctgg
    24841 gttatttgtt agtcccacga tttttttagg atcgatctcc caaagctgtt tgggaatatg
    24901 tgcttgtggg atcaaaggta aattggcaac ttttaaattt ttgttagcta aaaacaagct
    24961 caaaggcgtt ttggaagttc gggatacgcc tagcaaaacg acatctgctt ctaaaaaacc
    25021 acgaggatct ttcccatcat cgtattttac agcaaattcc attgctttga tccgtttgaa
    25081 atagttctca ttcaataaat ggagggcacc aggttgccga attggcttga taccagttaa
    25141 acgttcaatt tctgatatcg gtggcgtcat cacatcaaaa ctaaataatt gatgttcttc
    25201 acaaaaagaa cgtgttatct cgatcagttc ttgattgatt aatgtatgca ggacaagtgc
    25261 tttttcttct aatgcatctt gcaatgcctg ccttaatgtt ttttcatctg tcacgaacgt
    25321 tctgcggaat aggttgaact caacggtagg atactgagcc atagtagctt gcgctagttt
    25381 tgtcgcagtt tctccagcag agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc
    25441 ggaagtcatg gataaaacct cacttgcatt ttttttatgt ttattatacc atactctaga
    25501 atgcgtactt cgtaatcatt ttgatttgga gggataagtc atgaaaaaac agacattgat
    25561 cgaacaatca ttagaaacat tgaaaaataa cggttttaaa tatactaaaa aaagagaagc
    25621 attgttaact tatttagtga agcgtaaccg ctacgtatct gctaaagaag tttttgacta
    25681 tatgaatcag gaattcaaag gggtaagtta tgatacgatt taccggaatc tccatgattt
    25741 tgcagagatc gatttactgg aagaaaccga tttaaatgga gaaatgaagt ttcgttttca
    25801 ttgctgtcag gggaaaatcg gccatcacca tcattttatc tgtacagtgt gcggaacaac
    25861 gaaagaaatc catatgtgtc cgatgaatta tttcgaagaa cagctaggtg gatgcacgat
    25921 cgaagggcat agattcgaga ttttcggaag atgtgaaaat tgtcaataag gatttaaata
    25981 aaataacatt tgataatttt aaaaaaaagc aagatttcct tgacggtcaa aacagcatta
    26041 gatataatat ttacaggaca gtcttgtttt ttgagatata tttcaataac aattacggtt
    26101 actttttaag ctcggaggga gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa
    26161 tcacttgacg atgctcttcg tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa
    26221 gaatctcgta aacgtgaatt ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa
    26281 gcagctagaa aacgcaaaaa attctagttc tgtgatgaat tggagttggt agtgtgtcac
    26341 tactaagtag attaaacgat gacatgaaag cagcgatgaa agcgaaagat aaggaatctt
    26401 tacaagtcat ccgaatgatc aagtcatcta tacaaaacga gcagattaaa gaaggtcacg
    26461 acttgacaga agaagaagaa ctaacggtgt tgtctcgtga aatgaaacag cgacgtgatt
    26521 ctttgcatga gtttgaagaa gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa
    26581 ttgtaatagt cgaaaaatat atgccggaac aactttccga cgaagaaatt cgacaacttg
    26641 ttcaagaagc aatcactcaa actggtgctt catcaatgaa agaatttggc aaagttatgg
    26701 gtgcaattat gcctaaagtc aaaggaaaag cagatggaaa ccaagtgaat gcgattgtga
    26761 aagaactatt acaatcttaa ttttatcatt taaactgcgg acagtgatta tccgcagttt
    26821 tttgtgttcg taaaaaaaga actaaaaaac agctagaaga aagaatctta ttctt
//
LOCUS       DACDIP010000036        25552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDIP010000036 DACDIP010000000
VERSION     DACDIP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.036"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            164..1969
                     /gene="glmS"
                     /locus_tag="IV257_001680"
     CDS             164..1969
                     /gene="glmS"
                     /locus_tag="IV257_001680"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8814787.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(2041..3396)
                     /locus_tag="IV257_001681"
     CDS             complement(2041..3396)
                     /locus_tag="IV257_001681"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8814788.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(3420..4589)
                     /locus_tag="IV257_001682"
     CDS             complement(3420..4589)
                     /locus_tag="IV257_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814789.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(4586..5473)
                     /locus_tag="IV257_001683"
     CDS             complement(4586..5473)
                     /locus_tag="IV257_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8814790.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(5662..9342)
                     /gene="nifJ"
                     /locus_tag="IV257_001684"
     CDS             complement(5662..9342)
                     /gene="nifJ"
                     /locus_tag="IV257_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8814791.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            9540..10061
                     /locus_tag="IV257_001685"
     CDS             9540..10061
                     /locus_tag="IV257_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8814792.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(10760..11325)
                     /locus_tag="IV257_001686"
                     /pseudo
     CDS             complement(10760..11325)
                     /locus_tag="IV257_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11504..12160)
                     /locus_tag="IV257_001687"
     CDS             complement(11504..12160)
                     /locus_tag="IV257_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814793.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(12728..13663)
                     /gene="whiA"
                     /locus_tag="IV257_001688"
     CDS             complement(12728..13663)
                     /gene="whiA"
                     /locus_tag="IV257_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8814794.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(13697..14695)
                     /locus_tag="IV257_001689"
     CDS             complement(13697..14695)
                     /locus_tag="IV257_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8814795.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(14692..15576)
                     /gene="rapZ"
                     /locus_tag="IV257_001690"
     CDS             complement(14692..15576)
                     /gene="rapZ"
                     /locus_tag="IV257_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8814796.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(15711..16442)
                     /locus_tag="IV257_001691"
     CDS             complement(15711..16442)
                     /locus_tag="IV257_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8814797.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(16446..17711)
                     /locus_tag="IV257_001692"
     CDS             complement(16446..17711)
                     /locus_tag="IV257_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8814798.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(17974..20790)
                     /gene="uvrA"
                     /locus_tag="IV257_001693"
     CDS             complement(17974..20790)
                     /gene="uvrA"
                     /locus_tag="IV257_001693"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8814799.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(20800..22794)
                     /gene="uvrB"
                     /locus_tag="IV257_001694"
     CDS             complement(20800..22794)
                     /gene="uvrB"
                     /locus_tag="IV257_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8814800.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            23053..24555
                     /locus_tag="IV257_001695"
     CDS             23053..24555
                     /locus_tag="IV257_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8814801.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            24557..25294
                     /locus_tag="IV257_001696"
     CDS             24557..25294
                     /locus_tag="IV257_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8814802.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT         7300 a         5576 c         4496 g         8180 t
ORIGIN      
        1 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
       61 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
      121 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
      181 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
      241 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
      301 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
      361 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
      421 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
      481 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
      541 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
      601 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
      661 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
      721 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
      781 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
      841 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
      901 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
      961 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
     1021 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
     1081 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
     1141 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
     1201 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
     1261 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
     1321 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
     1381 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
     1441 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
     1501 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
     1561 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
     1621 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
     1681 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
     1741 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
     1801 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
     1861 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
     1921 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
     1981 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
     2041 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
     2101 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
     2161 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
     2221 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
     2281 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
     2341 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
     2401 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
     2461 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
     2521 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
     2581 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
     2641 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
     2701 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
     2761 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
     2821 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
     2881 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
     2941 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
     3001 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
     3061 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
     3121 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
     3181 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
     3241 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
     3301 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
     3361 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
     3421 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
     3481 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
     3541 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
     3601 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
     3661 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
     3721 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
     3781 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
     3841 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
     3901 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
     3961 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
     4021 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
     4081 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
     4141 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
     4201 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
     4261 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
     4321 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
     4381 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
     4441 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
     4501 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
     4561 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
     4621 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
     4681 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
     4741 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
     4801 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
     4861 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
     4921 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
     4981 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
     5041 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
     5101 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
     5161 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
     5221 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
     5281 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
     5341 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
     5401 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
     5461 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
     5521 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
     5581 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
     5641 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
     5701 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
     5761 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
     5821 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
     5881 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
     5941 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
     6001 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
     6061 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
     6121 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
     6181 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
     6241 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
     6301 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
     6361 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
     6421 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
     6481 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
     6541 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
     6601 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
     6661 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
     6721 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
     6781 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
     6841 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
     6901 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
     6961 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
     7021 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
     7081 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
     7141 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
     7201 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
     7261 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
     7321 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
     7381 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
     7441 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
     7501 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
     7561 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
     7621 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
     7681 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
     7741 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
     7801 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
     7861 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
     7921 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
     7981 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
     8041 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
     8101 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
     8161 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
     8221 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
     8281 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
     8341 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
     8401 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
     8461 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
     8521 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
     8581 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
     8641 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
     8701 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
     8761 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
     8821 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
     8881 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
     8941 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
     9001 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
     9061 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
     9121 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
     9181 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
     9241 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
     9301 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
     9361 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
     9421 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
     9481 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
     9541 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
     9601 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
     9661 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
     9721 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
     9781 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
     9841 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
     9901 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
     9961 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    10021 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    10081 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    10141 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    10201 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    10261 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    10321 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    10381 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    10441 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    10501 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    10561 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    10621 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    10681 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    10741 atggattaat agatttcgat tattcaaagc gattttctat tggtgaattg tgttcccaac
    10801 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    10861 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    10921 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    10981 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    11041 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    11101 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    11161 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    11221 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    11281 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    11341 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    11401 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    11461 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    11521 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    11581 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    11641 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    11701 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    11761 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    11821 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    11881 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    11941 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    12001 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    12061 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    12121 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    12181 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    12241 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    12301 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    12361 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    12421 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    12481 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    12541 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    12601 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    12661 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    12721 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    12781 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    12841 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    12901 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    12961 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    13021 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    13081 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    13141 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    13201 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    13261 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    13321 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    13381 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    13441 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    13501 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    13561 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    13621 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    13681 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    13741 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    13801 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    13861 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    13921 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    13981 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    14041 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    14101 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    14161 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    14221 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    14281 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    14341 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    14401 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    14461 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    14521 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    14581 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    14641 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    14701 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    14761 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    14821 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    14881 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    14941 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    15001 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    15061 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    15121 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    15181 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    15241 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    15301 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    15361 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    15421 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    15481 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    15541 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    15601 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    15661 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    15721 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    15781 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    15841 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    15901 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    15961 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    16021 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    16081 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    16141 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    16201 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    16261 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    16321 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    16381 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    16441 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    16501 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    16561 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    16621 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    16681 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    16741 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    16801 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    16861 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    16921 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    16981 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    17041 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    17101 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    17161 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    17221 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    17281 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    17341 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    17401 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    17461 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    17521 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    17581 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    17641 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    17701 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    17761 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    17821 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    17881 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    17941 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    18001 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    18061 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    18121 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    18181 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    18241 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    18301 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    18361 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    18421 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    18481 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    18541 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    18601 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    18661 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    18721 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    18781 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    18841 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    18901 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    18961 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    19021 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    19081 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    19141 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    19201 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    19261 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    19321 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    19381 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    19441 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    19501 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    19561 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    19621 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    19681 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    19741 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    19801 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    19861 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    19921 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    19981 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    20041 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    20101 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    20161 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    20221 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    20281 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    20341 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    20401 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    20461 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    20521 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    20581 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    20641 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    20701 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    20761 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    20821 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    20881 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    20941 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    21001 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    21061 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    21121 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    21181 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    21241 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    21301 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    21361 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    21421 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    21481 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    21541 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    21601 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    21661 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    21721 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    21781 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    21841 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    21901 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    21961 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    22021 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    22081 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    22141 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    22201 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    22261 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    22321 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    22381 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    22441 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    22501 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    22561 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    22621 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    22681 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    22741 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    22801 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    22861 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    22921 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    22981 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    23041 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    23101 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    23161 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    23221 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    23281 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    23341 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    23401 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    23461 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    23521 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    23581 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    23641 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    23701 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    23761 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    23821 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    23881 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    23941 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    24001 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    24061 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    24121 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    24181 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    24241 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    24301 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    24361 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    24421 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    24481 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    24541 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    24601 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    24661 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    24721 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    24781 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    24841 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    24901 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    24961 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    25021 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    25081 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    25141 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    25201 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    25261 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    25321 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    25381 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    25441 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    25501 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aa
//
LOCUS       DACDIP010000037        25507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDIP010000037 DACDIP010000000
VERSION     DACDIP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.037"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            19..2184
                     /gene="acm"
                     /locus_tag="IV257_001697"
     CDS             19..2184
                     /gene="acm"
                     /locus_tag="IV257_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8814803.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2456..2941)
                     /locus_tag="IV257_001698"
     CDS             complement(2456..2941)
                     /locus_tag="IV257_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8814804.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3421..4062
                     /locus_tag="IV257_001699"
     CDS             3421..4062
                     /locus_tag="IV257_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8814805.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4081..4716
                     /locus_tag="IV257_001700"
     CDS             4081..4716
                     /locus_tag="IV257_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8814806.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4734..5549
                     /locus_tag="IV257_001701"
     CDS             4734..5549
                     /locus_tag="IV257_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8814807.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5868..7304
                     /locus_tag="IV257_001702"
     CDS             5868..7304
                     /locus_tag="IV257_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8814808.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7493..9940
                     /locus_tag="IV257_001703"
     CDS             7493..9940
                     /locus_tag="IV257_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8814809.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10170..11237
                     /gene="rlmN"
                     /locus_tag="IV257_001704"
     CDS             10170..11237
                     /gene="rlmN"
                     /locus_tag="IV257_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8814810.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11430..12533)
                     /locus_tag="IV257_001705"
     CDS             complement(11430..12533)
                     /locus_tag="IV257_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8814811.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13086..13634
                     /gene="aac(6')"
                     /locus_tag="IV257_001706"
                     /pseudo
     CDS             13086..13634
                     /gene="aac(6')"
                     /locus_tag="IV257_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
     gene            14180..15841
                     /locus_tag="IV257_001707"
     CDS             14180..15841
                     /locus_tag="IV257_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8814812.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15911..16258)
                     /locus_tag="IV257_001708"
     CDS             complement(15911..16258)
                     /locus_tag="IV257_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8814813.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16342..17283
                     /locus_tag="IV257_001709"
     CDS             16342..17283
                     /locus_tag="IV257_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814814.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17284..18327
                     /locus_tag="IV257_001710"
     CDS             17284..18327
                     /locus_tag="IV257_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814815.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18333..19403
                     /locus_tag="IV257_001711"
     CDS             18333..19403
                     /locus_tag="IV257_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814816.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19393..20346
                     /locus_tag="IV257_001712"
     CDS             19393..20346
                     /locus_tag="IV257_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8814817.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20358..21368
                     /locus_tag="IV257_001713"
     CDS             20358..21368
                     /locus_tag="IV257_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8814818.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21653..22474)
                     /locus_tag="IV257_001714"
     CDS             complement(21653..22474)
                     /locus_tag="IV257_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8814819.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22571..22849)
                     /locus_tag="IV257_001715"
     CDS             complement(22571..22849)
                     /locus_tag="IV257_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814820.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23117..25126
                     /gene="metG"
                     /locus_tag="IV257_001716"
     CDS             23117..25126
                     /gene="metG"
                     /locus_tag="IV257_001716"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8814821.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
BASE COUNT         8624 a         4415 c         5283 g         7185 t
ORIGIN      
        1 ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt
       61 attactaata taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc
      121 agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata
      181 aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg
      241 atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta
      301 atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg
      361 tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag
      421 gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt
      481 gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt
      541 tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat
      601 attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt
      661 ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag
      721 gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt
      781 aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt
      841 aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag
      901 aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc
      961 aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa
     1021 ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa
     1081 ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat
     1141 gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa
     1201 gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata
     1261 aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac
     1321 atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa
     1381 ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta
     1441 gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac
     1501 gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt
     1561 tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc
     1621 tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc
     1681 acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat
     1741 gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat
     1801 actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa
     1861 aacagttata caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg
     1921 aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg
     1981 caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga
     2041 gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa
     2101 aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc
     2161 ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa
     2221 tcaaaagaat aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt
     2281 ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt
     2341 tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt
     2401 gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca
     2461 aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat
     2521 ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa
     2581 tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag
     2641 tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata
     2701 agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca
     2761 agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg
     2821 gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt
     2881 gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca
     2941 tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa
     3001 taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt
     3061 aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat
     3121 cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg
     3181 gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg
     3241 gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa
     3301 cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt
     3361 ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag
     3421 atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa
     3481 ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg
     3541 tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga
     3601 acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt
     3661 tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca
     3721 gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt
     3781 ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt
     3841 gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat
     3901 atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact
     3961 ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc
     4021 gtaatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat
     4081 atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg
     4141 aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa
     4201 accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta
     4261 gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta
     4321 ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca
     4381 aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct
     4441 tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa
     4501 cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa
     4561 ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg
     4621 aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc
     4681 ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa
     4741 aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca
     4801 aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt
     4861 tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg
     4921 atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca
     4981 ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg
     5041 gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa
     5101 acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata
     5161 ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag
     5221 aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag
     5281 cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag
     5341 actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg
     5401 agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca
     5461 atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt
     5521 ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca
     5581 cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa
     5641 tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc
     5701 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta
     5761 agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt
     5821 tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag
     5881 aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag
     5941 aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac
     6001 ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat catcgttata
     6061 aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt
     6121 ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg
     6181 agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt
     6241 gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg
     6301 aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact
     6361 ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc
     6421 cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa
     6481 atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct
     6541 ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa
     6601 atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga
     6661 ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg
     6721 cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga
     6781 cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa
     6841 aaggttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg
     6901 tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc
     6961 gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg
     7021 acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc
     7081 acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct
     7141 ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat
     7201 atgttgatca agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt
     7261 actggtacca aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac
     7321 aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga
     7381 aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta
     7441 gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca
     7501 aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca
     7561 gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg
     7621 ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg
     7681 aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa
     7741 gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt
     7801 cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa
     7861 tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa
     7921 taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc
     7981 acagcctgga agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag
     8041 taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga
     8101 attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat
     8161 gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa
     8221 agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga
     8281 tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag
     8341 ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca
     8401 gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga
     8461 atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct
     8521 tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg
     8581 cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt
     8641 aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat
     8701 tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc
     8761 accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt
     8821 ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc
     8881 tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt
     8941 actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag
     9001 tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt
     9061 tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag
     9121 aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt
     9181 cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt
     9241 ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc
     9301 tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat
     9361 ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat
     9421 cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac
     9481 agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat
     9541 gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga
     9601 ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga
     9661 gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga
     9721 gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct
     9781 ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc
     9841 tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga
     9901 agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt
     9961 gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt
    10021 tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt
    10081 tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc
    10141 gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga
    10201 atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag
    10261 tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga
    10321 aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg
    10381 tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga
    10441 ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag
    10501 gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga
    10561 ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc
    10621 ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg
    10681 acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca attggtgctc
    10741 ggcacattac agtttccact agtggattag cacacaaaat caaggaattc gctaataacg
    10801 gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga
    10861 tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt
    10921 tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata
    10981 gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg
    11041 tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag
    11101 ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta
    11161 aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa
    11221 aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt
    11281 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
    11341 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
    11401 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
    11461 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
    11521 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
    11581 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
    11641 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
    11701 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
    11761 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
    11821 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
    11881 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
    11941 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
    12001 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
    12061 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
    12121 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
    12181 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
    12241 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
    12301 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
    12361 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
    12421 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
    12481 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
    12541 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
    12601 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
    12661 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
    12721 taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc
    12781 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
    12841 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
    12901 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt
    12961 attttataag taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt
    13021 tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa
    13081 gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg
    13141 atttactgag actgacttgg ccggaagaat atggagacag ctaggcagaa gaagtagaag
    13201 aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat
    13261 ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag
    13321 aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag
    13381 tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa
    13441 cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc
    13501 ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac
    13561 caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac
    13621 caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag
    13681 gaatttacga aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct
    13741 acttttggaa caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga
    13801 aaaattttgt gaaaatggct ttctacattt ttgcataaat ttggcttaat accgagaaac
    13861 tggcttttga acaccgttta ttcagataag ggactgtgac aagacttttg tcacagtttc
    13921 tttttgttta ttctttttta tctttgtatg gcaataatag acaatataac gaaaattatt
    13981 taatggcttt tggaagacca tggaaaacct tgtacaaagc tattttttct gaaaaattcc
    14041 ttgaaacctc acgaaaaaat cattatttta tgaaaaagac aaaaaaaagt gttgattttt
    14101 taataattat tcattaaact cttaaacaac ttcagaaaat attaattatt ctgaaaattc
    14161 caataaaggg gtacgtaaaa tgaaaaagaa atttacattt ggtttggttg cggtatgcgg
    14221 ccttgtactt gctggatgtt atggcggcag ttcagccaca agcagtagtt cagcaaaaag
    14281 cggttcagca gatggaggag gcgtatttaa cttagtcgtg cctcaagaaa tgcctacagc
    14341 tgacttatca gttgcaacag atacaatcag ttttacagca ttgaataacg tttatgaagg
    14401 aatttaccgt cttgatgaag acagcaaacc acagccggca ggagctagcg agttggcaga
    14461 agtcagtgaa gacggattaa cttacaaaat caaattacga gaagacgcaa aatggtcaaa
    14521 tggtgatcct gttacagcag cggattacgt ttatggctgg caacgaacag tagacccagc
    14581 tacagcttct gaatatgctt acctgtatgc gccagttgaa aatgcagaag acattacagc
    14641 tggaaagaaa gataaatcag aactagggat caaagcagtt ggtgactatg aattagaaat
    14701 caaattgacg aaacaaacac catatttcca atatctgctt gctttcccat catttttccc
    14761 acaaaaacaa tccgtagtgg aagaaaatgg ggatgcctat gcatctgcaa gtgataaagc
    14821 tgtttataat ggaccattca cattagaaga ttttgatgga ccaggaacag atacagaatg
    14881 gacttataag aaaaatgatc attactggga caaagataca gtaaaacttt ctgaaatcaa
    14941 agtcagtgta gtaaaagaat cttctactgc attgaactta ttcaaagatg gccaagcaga
    15001 tgacgtgatc ttgtctggtg agttagcaca gcaaaacgca aacgatccag cttatacttc
    15061 tgttaaagaa gcgcggacaa gctatatcga attcaatcaa cgtgaaaaga attcaccatt
    15121 caacaatgtg aacctaagaa aagctatttc ttattctatc gatcgtgaag ctttagtaaa
    15181 acaagtacta ggtgacggat cagttgtctc tacaggattg atcccatcag atatggtaaa
    15241 aagtccaaca acgaacgaag actttgcaaa agaagctgga aaattagttt cttatgacaa
    15301 agataaagca aaagaatatt gggaaaaagc gaaaaaagaa ttgggcgttg attctttaga
    15361 atttgatttg atggcttctg atgatgattc atcgaaaaaa gtaatcgaat atgttcaaaa
    15421 ctcaatccaa gaaaacttgg atggcgtaac agtcaaacca acacctgttc cattctctgt
    15481 acgtcttgac cgctcaactt cgggtgattt tgacgcagta ctaggcggat gggctgctga
    15541 ctatgcagac ccaagcagct ttactgattt gtttgtcaca ggtaattcat acaaccgtgg
    15601 tcgctggagc aatgcagaat atgacgaagc agttaaagca gcttcatcaa cagacgctgg
    15661 cgatgaacaa gcccgttggg atgatttcca aaaagcaatc aagatcatct ctgatgatat
    15721 gggtgtcgtt ccggtgtacc aaaaagcaga aggtcaccta gtgaatgaaa aaatcaaagg
    15781 tatcgttcac catgcggcag gcgcttcatg ggattacaaa tggacatacg ttgaagaata
    15841 atcgttaata ccgtgagcat gagaaaaaac gaggctgcgg caaatgtcgc aacctcgttc
    15901 tcatattcac ttatcctaac aaagaaaaaa gcatgaaaat agcagaaaga gcaattagta
    15961 ttccggcaat gatcagccaa aagctgtaca taccgtagct ggcagacgag agcgaggaaa
    16021 attctggttt tttctttcgg ttccttgttc gtgcaagatg gaccgatcgc tgaaaatgat
    16081 cgaagaaacc cgaagaaaag acccataaaa aaccgccgat aatcagaaac ggcaatgacc
    16141 ataagaatag atcattagat aattgaatga gtgtaaagga atggttgatg agatttttta
    16201 ttaaaataac cagaatcagt agaaaagcta ctgcgtaggg gatagatttt tttttcattt
    16261 gattgctcct ttatttttgg ttcattatct aataatgtag cgaaacatct gtcagaagtc
    16321 aaaagctagg gggaaacatt agtgaatagt tatatcaaat atgtattaaa acgtgtcttt
    16381 ttcatgatca tcacgctttg gttaattgca acaatcacct ttttcttgat gcaattgctg
    16441 ccgggcacgc catatacgaa tcaggaacgt ttgagtcctg aaacaatcga gatgctaaat
    16501 aaacaagtcg gtttggataa accagttatc gtacaatatg ggatttatct ttctaatttg
    16561 cttcaaggag attttggaat ttccttccaa tttaaaaacc agccagtcgc acatttgtta
    16621 gctggaagaa tcggaccatc cttgcagtta gggttacagg caattatctt tggaacagtc
    16681 ctagggacga ttttaggaac gatctctgcc atgaagcaaa atacatgggc agatacatct
    16741 tctacactcg tagcgatatt aggtcgttcc attccaaact ttgtatttgc tgttttatta
    16801 caatatatct ttgcaatcaa attacaagtt ttgccaatcg caaaatggga tggctttgtg
    16861 tacacaatct taccaacaat tgcgttggcc atgtcgccgc ttgctgactc tgctcggttt
    16921 atccgtacgg aaatggtgga agtactgcat agtgattatg tggaattagc aaaagcaaaa
    16981 ggattgagcc gttgggaaat cgcctttaaa catggtcttc gaaatagttt gattccattg
    17041 atgactttat taggaccgtt agctgtagca ttgatgaccg gatcactggt ggtagaaaat
    17101 atctttgcta ttcctggtat cggggaacaa ttcgttaagt caatcacaac aaatgattat
    17161 cctacgatca tggctgtaac gatcctttac tcatttatgc tgatttttgt tattttgatc
    17221 gtggatctat tgtacggtct agttgatcca agaattcgtg tttcagaagg gagccgagga
    17281 taaatggaaa tgataccaaa gcgttatgaa acagttgcag atatccctgc ggatgaattt
    17341 cttccattac aaaaaaatac cgaagaagaa cgtgaacaga ttactgcacc ttctttgaat
    17401 tttatccaag attcatggcg ccgtttaaag aaaaacaaag ctgccgttat ttcaatggtt
    17461 ttactattgg tcatcatttt gatctctgtg atcacaatct ttgtttctcc tcatgatcca
    17521 actgcccaaa acgtggctta tatcaatttg cctcctcgta ttccgggaat caatatcgat
    17581 ggattgaatg gaaaagcaat ggttggtggt gaacttgttg ataaatatgc tcaagcaaac
    17641 gtcccttcaa acgtaaattt ctttttagga acagacggtt tgggacgtga tgtattaagc
    17701 cgcttattta tgggaatgag aatctcgttg ctgatcgcat ttattgctgc attactagat
    17761 gtaacgattg gtgtagctta cgggttggtt tctggtttgt taggcggacg tgtcgataac
    17821 gtgatgcaac gttttttgga aatcttatca ggaattccaa acctcgttgt catgattttg
    17881 atgttggtcg tttttgaacc aggaattttt tcaatcgtgg ctgctatggc catcacaaac
    17941 tggataccaa tggcacgtat cgttcgtgca caaacaatga aattaaaaga tcaagaatat
    18001 gttttagctg gattgacatt aggtgaatca agaatgaaaa ttgcattcaa acatattttg
    18061 ccgaatatct ctagtgtcat tattattcaa atgatgttta gtattccatc tgctattttc
    18121 ttcgaagcct ttttaagctt tatcggttta ggtcttacgc ctccgtcagc ttcattagga
    18181 acgatgttga gcgaagggta caagacattc ctgtatctgc cttatctatt atggattccg
    18241 gcagtaactt tatcggttat catgattggc tttaacttac ttgctgatgg attgcgtgat
    18301 gcctttgatc ctaaaatgaa agagtgaaga cgatgacgaa aattttagaa gtgaaagact
    18361 tagaaatctc ttttgatacg tatgctggaa aggtacgagc gattcgcggt gtcaattttc
    18421 atttgaataa aggagaaaca ttggcgatcg ttggggaatc tggaagtgga aaatcagtga
    18481 cgaccagaag catcatgcgc ttgctgtcaa gcaacgccaa tatagatgct ggtcaaatcc
    18541 tctttaaagg taacgatatc gttggaaaat cagaaagaga gatgcaaaaa atccgtggta
    18601 aagaaatagc gatgattttc caagatccaa tgacctcttt agatccgacg atgcccattg
    18661 ggaagcaagt cgctgagtcc cttagaaagc ataataaagt ttcaaaaaaa gagggacaaa
    18721 aagctgcatt agaattgctt aaattagttg gaatacccga agctgagaaa cgtatcaaca
    18781 gttatccgca tcagttctca ggaggtcagc gtcaacggat cgtgatcgcg attgcactgg
    18841 tatgttatcc ggaaatcctg attgctgatg aacctacaac agcgcttgat gtgactattc
    18901 aagcacagat tttggagctg ctgaaagaaa ttcagacaaa aatcgataca tcaattatct
    18961 ttatcactca tgaccttggt gtagtagcga atgtcgctga tcgtgtcgct gtcatgtatg
    19021 gaggaagaat cgttgaagtt ggaacatcgg aagaaatttt ctacaacccg caacatccct
    19081 atacttgggg actgcttggt tcaatgccga cattagatag tgcgaacgat cgattgtatg
    19141 ctattcctgg ttctccaccg gatttattga acccgccaaa aggagacgca ttctatccaa
    19201 gaaatgaatt cgcgatgaaa atcgatgcag aacaagaacc accatttttt gaactatcaa
    19261 aaacacacaa ggcagcaact tggctacttg ctccgcaggc acccaaagtg acaccgccag
    19321 aagaaatcca acgtcgctgg gctatcttta aagagaaaca aaaacatact tacggtcaag
    19381 gaggaattgc tgatgccaaa taaactactt gaagttaaag gattgaaaca gtatttcaat
    19441 gtaggaaaaa agaatgaagt acatgctgta aatgacatca gctttcacat ttatgaagga
    19501 gaaacattcg gtttagttgg agaatctgga agcggaaaat ccactactgg ccgtacgatc
    19561 attcgcttga atgaacctac tgatggtgag atattattcg acggtcaaga tataacgaag
    19621 atcaaagata aaaaaggttt gacgaaattc cggcatgatg ttcagatgat ctttcaagat
    19681 ccatacgctt cgttgaatcc tcgtatgaaa gtcagagata tcattgccga aggaattgat
    19741 gttaatggac tagctaaatc atctgaagag cgtgcaaaaa aagtcgatga tctattgaga
    19801 actgttggat tgaatccaag ccacggtact cgttatcctc atgaattttc aggtgggcaa
    19861 cgccagcgta ttggtattgc acgagcattg gcagtaaaac cgcgttttat catttgtgat
    19921 gaaccaattt ctgcactaga tgtgtcgatc caagcccaag tggtcaattt gttgcaagat
    19981 ctccaaaaag aacatcaatt gacctatcta tttattgcac atgatttgtc gatggttaaa
    20041 catattagtg accgaatcgg tgtaatgcat aacggcttgt tattagagat gggaacaagt
    20101 gatgaaatct ataatcacgg tgttcatcct tatacagaaa gtttgttatc tgctattcct
    20161 ctaccagatc cagaccatga gagaaaacgc agaagaatca aatatcaacc tgaaccagat
    20221 gacggacaag tacgtcaact tcgtgaaatc gcaccagaac attttgtcta tgcaactgag
    20281 caggaagtcc catattacgc aaaaaaacta aaacgacaaa aggaatctct tctggttgct
    20341 aactaggagg gacatagatg ctaaaagctt ttaaattccg tatgtatccg acagaagaac
    20401 aaaaacagca attgattcgt acttttggat gtgctcgatt tacctataac catttgctga
    20461 aaaagcggca gaaaagttgg caacaaacag gagtagccaa tttttcgctc acacccgcta
    20521 cattaaaaaa agaataccca ttcttaaaag aagtagacag tcttgcttta gcaaatgcac
    20581 agcttaattt ggatcgggct tttcgcaatt atttcaaggg acgtgccagc ttcccaaagc
    20641 ttaaaacgaa aaaaagtatg tggcaatctt atacgaccaa caaccaaacc cgtacgatct
    20701 atctggaaaa tggctactta aagttaccaa aacaaaaaga actaatcaag ataaatcagc
    20761 atcggccagt tgaaggatcg attcgctccg ctacgatttc tgctcgatat aatgaagaat
    20821 tttatgtcgc actactttgt gatgtttcgc cagtaaaaaa agaatcttta gctaaatgga
    20881 tcgggatcgc ctatcatccg aagacattga tccaaacatc tcgacctcta gaagtaactt
    20941 taccaaaatt tcaccagaca gaagaaaagc tgcaacatgc acagcggaaa ttgaatgtga
    21001 aagtccgctc cgctcaccat agaaagatca ggttagatca ggcaagcaat tatcagaaac
    21061 aaaagagaaa agtaatggat ctttatctga aacaaaagaa ccagcgagaa gattatcttg
    21121 aacaattatc tgggaaattg gtcaaacaat atgactactt atttgtagaa tcttttccaa
    21181 aagaagaggc acatgctgat ttttctattc acgactggca taaattaata acaaaattgc
    21241 gttataagtc ccaatggtat aataaaaaat ttcttttgat caataccgac ggagcagaag
    21301 aaagcaattc cgtaagaaag agccaagtag tagaaaaaat gggcagacat tcggttatca
    21361 agggataaaa aatgaatgaa actgatagga gcgttagggt tagccttgtt aataaaaagt
    21421 cttaatgact tttgtttcta ggaatttttc ttttgaagaa aagctcacta attatgacaa
    21481 accaataaaa caatagcaga aaaagttcgt acgagctttt tctgctattg ttaatttttt
    21541 attcagaaag tttacgaata catcaaaatt ctgaaacaaa aaacgtaact cacaaagagt
    21601 tgtttttgcg ttttttcttg aatgattagg tctgaatctt ttgctcacaa ccttattcat
    21661 ggtgccccgg cttgagctga tagatttttg aaggacggcc gattcctaca ggacgttcac
    21721 cgatctcatc gaaatattgg agcatagctt tcttaaaatt ggaatgatcg atcgttttat
    21781 aatcgatccc taagaattta gcaaatactt ttcgagcttc cgtaatcgtg aaatctttac
    21841 ctaaaacttg tagaacttgc ggctcgtgtt ccattttatt gactacacgg ttgaatgctt
    21901 tcaagatgat ttgactatgg tcaaaagcca aagtatcttt gccagaagac tctccagtga
    21961 ctaaatcaag cgagatagcc acttctccgc tagataaatg gatcttgtta ctatgacgtt
    22021 ctagtgtgaa ccagcgtact tccttggcat catctccggc gattagcggt tcttctccaa
    22081 taaaggctaa gtagctgact gtgacgaccc agcctcgcgg atctcgatta ggcgtactga
    22141 atgtatgcag ttgctcaata ttttcttttg aaataaccac accagtttct tcctttgttt
    22201 ctcttaagac gctttctcca gttgactcat ccttttggac aaagccacca ggcaatgccc
    22261 atgaatttcg ataaggatgc ccttttcttt ggatcaacaa aactttcagc tgatcttctt
    22321 ctttgttata gcacatcagt acgatatcga cagtcaaaga gggcttgtca tattctggac
    22381 gttcctgttt ttgataccag ttaagaaatt cttcttcact tgctttatgt tcataatata
    22441 atttttcttc ttctttagaa gcaaaacggt tcatctgacc acctccattg acagtaaaag
    22501 tactttttac cttattgtag cagactctga aaaaaaacca aggatttact tgaaaaatct
    22561 aggttatcat ttaaatatga aaaataaatg gaagtatgag attgaataaa aagaaaagca
    22621 gataaaacgg caatacttta taccgctctt cacgaatggc aaagaagcca caaataacga
    22681 tatatacgtc aatgataaaa cgcaaaatcc aagctgctgt cataaaactg ttcaaatgcc
    22741 catggaggtt aaaataacca tacatacgaa tataaaaata gctgaatatt aaatgtgcta
    22801 atgaaagaaa agcaatacta agtgtaaaaa aaagatgttt cttctgcatg attgttccag
    22861 tcccttacta agatagatag tctttattat accacgtgtg aggggagaaa taaaaagccg
    22921 ttttttagta gatatgaaac aaaacaaaaa cccgcattta ttcaacctat tcatcctttt
    22981 aaaggataga ttgaacgtag atagaagtcg tttatccagt ctttttcatt ttgaaaaaaa
    23041 gctaacagca tgcttttttt atggtatact agcaaggaaa cgaattcgca tcatgcgata
    23101 aaggaggaag agccccgtgg cagaaaagga aactttttat atcactacgc caatctatta
    23161 tccaagcggc aaacttcata tcggcaactc gtatactacg attgcatgtg acgcaattgc
    23221 tcgttataaa cggttgatgg gctttgatgt gttttatctc actggagtgg atgaacatgg
    23281 tcaaaaaatc gaaacaaaag cagaagaact aggtgtaaaa ccgcaagaat atgtagataa
    23341 aatggcggct gatgtcaaga aattatggaa aacgcttgat atcagttatg ataaattcat
    23401 tcgcacgaca gatgattacc ataaagcagc tgttcaaaaa atatttgatc gtttattaga
    23461 acagggagat atttacttag gtgaatatga aggctggtac tctgtttctg acgaagaatt
    23521 ctttacagaa acacaacttg cagaagtgta caaagatgac gaaggtaacg tgatcggtgg
    23581 taaagcgcca agtggacacg aagtagaatt ggtaaaagaa gaatcatact tcttccgcat
    23641 gagcaaatac gcagaccgtc tattggaata ctatgaagaa catcccgaat tcatccagcc
    23701 agaatctcgt aaaaacgaaa tgatcaacaa ctttatcaaa ccaggattag aggatcttgc
    23761 agtttcacga acaactttta cttggggagt accagtcaag agtgatccga aacatgtggt
    23821 gtatgtatgg atcgatgcat tatccaatta tattactgca ctaggatatg gctccgaaga
    23881 tgaaagtcta ttcgaaaaat actggcctgc agatgttcag atggtcggta aagaaatcgt
    23941 tcgctttcat acgatctatt ggccaatcat gctgatggcc cttgatttgc ctctacctaa
    24001 aaaagtattt gggcatggct ggctattgat gaaagacgga aaaatgtcta aatcaaaagg
    24061 aaatgtcgtt tatcctgaaa tgcttgtgga acgttatggt ttagatgcac tccgttatta
    24121 tttgcttcgt gctgttccgt ttgggtcaga tggtgtcttt acgccagaag attttgtttc
    24181 ccgtgtcaat tatgatctgg caaatgatct agggaactta ttgaatcgaa cgattgctat
    24241 gatcaacaaa tattgtgatg gactcgttcc tgactatgcc tcacttgtca ctccatttga
    24301 tagtgaactg tctacgacgg ctgctaacgt tgtcggacgt taccatgatg caatggaaaa
    24361 aatggaattc aatacagcat tggctgaaat ctgggtgctg atttctcgtg caaacaaata
    24421 cattgatgaa acagaaccat gggtattagc aaaagacgaa gaaaagaaag ctgaattgaa
    24481 tagtgtcatg atccatttag ctgaatcttt gcgtatcgca gcgatattac ttcagccaat
    24541 catgacagaa acgccagaga agatcttcaa tcaactagga ttggactctg aaacaatgaa
    24601 tcttgaagga ttacactttg gcgaatttcc ttcaggaaca aaagtagttg caaaaggtac
    24661 accaatcttc ccacgtttag atatggaaga agaagttgct tttattcaag aaaaaatgtc
    24721 tgaaggtact caaacaaacg aagatacagt caaatgggat ccagaagaaa cagaacttgt
    24781 ttcaacgaaa gaaaaacaaa tcaaatttga tgtatttgaa aaagtagaac taaaagtagc
    24841 agaagtcatc aactgtcaaa aagtagaagg ggcagataag ttgctgcaat tccgactaga
    24901 tgctggagac agccaagacc gtcaaatctt gtcaggaatc gctgaatttt atcctgatcc
    24961 aagcgaattg atcgggaaaa aagtcgtcat tgtagcaaat ctgaaaccac gaaagatgcg
    25021 tggacaaatc agtcaaggaa tgatcctttc tgcagaagca ccagatggct ctttacaagt
    25081 aatcgaagca ccaaaatcca tgccaaatgg ctcagaaatc gcatagaaat tcaaaggggg
    25141 tatgacacta gttgtgtcat accccctttt tccgaaattt gagaagcaat tttcgtaaat
    25201 tccttcttat tacttgaagc caatcatata atacagaaac agagaactat ccaaaataaa
    25261 tcctagaaaa agaagtacga gcttcgctga tttttgttaa tttagaataa tactaatagg
    25321 aatcgtaaaa acagaaaaaa agcataatga taaagggtcg ggcagatttt aattgattca
    25381 tctggtactc ttttttattg tccttacatt cataaagttt gaatgcaatg actttattac
    25441 atctatattt atatctaaca gtagcgttga tgtgttaatt ataagttcta tcactttatg
    25501 ttcaatt
//
LOCUS       DACDIP010000038        24798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDIP010000038 DACDIP010000000
VERSION     DACDIP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.038"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..227)
                     /locus_tag="IV257_001717"
     CDS             complement(99..227)
                     /locus_tag="IV257_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8814822.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(230..1603)
                     /gene="rlmD"
                     /locus_tag="IV257_001718"
     CDS             complement(230..1603)
                     /gene="rlmD"
                     /locus_tag="IV257_001718"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8814823.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1714..2769)
                     /locus_tag="IV257_001719"
     CDS             complement(1714..2769)
                     /locus_tag="IV257_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8814824.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2779..4209)
                     /gene="gatB"
                     /locus_tag="IV257_001720"
     CDS             complement(2779..4209)
                     /gene="gatB"
                     /locus_tag="IV257_001720"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8814825.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4206..5678)
                     /gene="gatA"
                     /locus_tag="IV257_001721"
     CDS             complement(4206..5678)
                     /gene="gatA"
                     /locus_tag="IV257_001721"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8814826.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5678..5983)
                     /gene="gatC"
                     /locus_tag="IV257_001722"
     CDS             complement(5678..5983)
                     /gene="gatC"
                     /locus_tag="IV257_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8814827.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6089..8125)
                     /gene="ligA"
                     /locus_tag="IV257_001723"
     CDS             complement(6089..8125)
                     /gene="ligA"
                     /locus_tag="IV257_001723"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8814828.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8142..10376)
                     /gene="pcrA"
                     /locus_tag="IV257_001724"
     CDS             complement(8142..10376)
                     /gene="pcrA"
                     /locus_tag="IV257_001724"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8814829.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMISYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10531..12090
                     /locus_tag="IV257_001725"
     CDS             10531..12090
                     /locus_tag="IV257_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8814830.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12201..14132)
                     /locus_tag="IV257_001726"
     CDS             complement(12201..14132)
                     /locus_tag="IV257_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8814831.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14150..15064)
                     /gene="pfkB"
                     /locus_tag="IV257_001727"
     CDS             complement(14150..15064)
                     /gene="pfkB"
                     /locus_tag="IV257_001727"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8814832.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15061..15813)
                     /locus_tag="IV257_001728"
     CDS             complement(15061..15813)
                     /locus_tag="IV257_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8814833.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15972..16181)
                     /locus_tag="IV257_001729"
     CDS             complement(15972..16181)
                     /locus_tag="IV257_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814834.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16192..17520)
                     /locus_tag="IV257_001730"
     CDS             complement(16192..17520)
                     /locus_tag="IV257_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814835.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17885..18472)
                     /locus_tag="IV257_001731"
     CDS             complement(17885..18472)
                     /locus_tag="IV257_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8814836.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18555..19805)
                     /gene="clpX"
                     /locus_tag="IV257_001732"
     CDS             complement(18555..19805)
                     /gene="clpX"
                     /locus_tag="IV257_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8814837.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20052..21341)
                     /locus_tag="IV257_001733"
     CDS             complement(20052..21341)
                     /locus_tag="IV257_001733"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8814838.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21495..22427)
                     /locus_tag="IV257_001734"
     CDS             complement(21495..22427)
                     /locus_tag="IV257_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814839.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22584..23243)
                     /locus_tag="IV257_001735"
     CDS             complement(22584..23243)
                     /locus_tag="IV257_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8814840.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23246..24271)
                     /gene="sppA"
                     /locus_tag="IV257_001736"
     CDS             complement(23246..24271)
                     /gene="sppA"
                     /locus_tag="IV257_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8814841.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24284..24574)
                     /locus_tag="IV257_001737"
     CDS             complement(24284..24574)
                     /locus_tag="IV257_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814842.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
BASE COUNT         6608 a         5356 c         4354 g         8480 t
ORIGIN      
        1 attttgtaca acccatcagt ccttattgac aaagagccac taaaaagact aagaaacaat
       61 aacaaaagtt aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg
      121 ccttttctca tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca
      181 ccttgtttct ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt
      241 taacgaatga agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa
      301 ctttcttcac ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg
      361 gattacatga gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat
      421 gatgatctaa tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt
      481 cttgccattt tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt
      541 tattatttaa agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat
      601 acaccttatc cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt
      661 cgatcacaat gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact
      721 ctgcttgttg agtatttact tgataaaatg actttgctga gattcgatag gtttttccta
      781 atagctgatc ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca
      841 catttgtttt ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat
      901 gaatagcttc agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga
      961 ccatcatttc atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat
     1021 ttttttcttc attatatcct cgtacttgga accgttgcaa gatatctctg actactttga
     1081 ttgcttgatc gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc
     1141 tattcttttt gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg
     1201 ctttattacg gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg
     1261 gcatcttagc aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt
     1321 gttcatactt caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta
     1381 caggctgacg atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat
     1441 tgcctgcttt tactactagt atttctactt cttctccagg gagtgcattt tcgataaata
     1501 atggataacc atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga
     1561 ctgtatatcg ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc
     1621 ctatttttca atcctactga actctactat aaatcagcta attacacaac taaaaccggc
     1681 actgacctta ctacgttcag caccggcttt tgattaattc ttttgattag gtgtttcgtc
     1741 tgctattttt ccgtcaccgt cgatatcttc atccgttaaa cgttcgactt cttttacgaa
     1801 ttctttgctg acaacctcta attcttcttc atctgtacct gtgattgctt catcaggaat
     1861 ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga ttttggaaat gcatcggtgc
     1921 ttccccgccg tattcaccat ctaagttgat cattactggt cgattgatat catcttctgt
     1981 ttcaacgaac aaactgcttg ttttggtata gattattctt ggatcatcga catgtcgacc
     2041 gccattcagc atgagtgctg caatatgcaa gatctcaaag atatttgctg tcttcacaat
     2101 gattaaagaa aacttcccat catccaattt ggcatctggc gcgatctgtt cgaatcctcc
     2161 aacagagttc gtcagcccta ggaaaaacat cgaagcattt ccgatatatt ccccttcatc
     2221 atacttcaag cgcattttga ttggctttac tcgagggagc atctctgctc ctttcgcaag
     2281 ataagcaaga taaccaaaaa tactctttag ttctgatgga acttcataag tcaattctgt
     2341 caaatagcca cctgcagcga tattgatgaa gtaactgtta tcagaaaggc cgatgtccat
     2401 ttttacggtt tggtgttttt ttatcacttc tgctgctgct ttaatattat ctctaggaat
     2461 ttttaatgct cgagcatagt cgttcgttgt accagctggg atgatggcca ttttaggacg
     2521 gtgttcaagc ggagcgattc cattaaccac ttcgttgatt gtgccatcgc caccagctgc
     2581 cactactaga tcaaaaccta gttctgcgac tctacatgct tcattcttgg ctgaatctgg
     2641 ttctggtgta gttgcatagg cgctggcttc aaatcctgct tcttctaatg cctgtaggat
     2701 atccgccaaa ttttttttca ataattcttt tcccgaggtg gggttataaa ttacgcgtgc
     2761 ttttttcatt ttcagcctct atcgtttcgc taattcatct ttaagtagct tattcacgac
     2821 accaggattt gcttgtcctt tagttgcttt catgatttga ccgactagga agccaaccgc
     2881 acgatctttt ccatttttga aatcatcgac agattgttgg ttgttatcca aaatttcatt
     2941 aatgattggc agtagtttgt tcggatcaga taattgcacc caaccatttt tttcaactac
     3001 ttcttgggct tctccgccat ttgtgatcaa ttcacggaag acttttttgg cgatttttga
     3061 gctgatcgtt ccatcggcga tcaaacgaat cattcctgct aagttctcag gtgtcaattt
     3121 tgtctcagct aattccagtt tctcactgtt caagtaagca gaaacttcac ccatcaacca
     3181 gttagatgct tgcttggcat ctgcaccatt ttccaaaaca gcttcaaaaa aatcagacat
     3241 ttcttttgta agtgtcaata ccattgcgtc atactctgga agtccaaact cacggatata
     3301 gcgttcgcga cgagaagctg gcatctcagg caaactagct tttacttttt gaatccattc
     3361 gtcatcgatc acgaatttcg ggatatcagg ctctgggaag taacgataat cactagaccc
     3421 ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg tcaaaacggc gtgtttcttg
     3481 ttggatttct cctcctgaaa gaagaacttt tgcttgacgt ttttcttcaa aagctaaacc
     3541 ttttttcacg aaagataaag agttcaagtt tttcaattct gctttcgtac cgaactcttc
     3601 ttgtccataa gggcgaagag aaatattggc atcacagcgc atagagcctt cttccatttt
     3661 cacatcgctg acttctgtaa attggataat tgatcgcaat gcttctagat aagcgtatgc
     3721 ttcttctggt gaacgcatat cagcttcaga aacgatttcg atcaaaggcg tgccttgtcg
     3781 gttcaaatca acgtaagaat aaccgccaat accatgcatg ttttttccag catcttcttc
     3841 taaatggaca cgttcgatcc ggattttttt ctttttacct tctacttcga tttcgatcca
     3901 tccgtcatga ccgattggtt gatcaaattg ggaaatttga tatgcttttg gattatctgg
     3961 gtaaaagtaa tttttccggt caaaatgagt atctttagag atttcacagt ttaatgccaa
     4021 agctgctttc atcccgaatt ccagcgcagc tttattcatt actggcagca cgcctggata
     4081 accccagtcg atcacgtttg tattgctgtt tggttctgca ccaaaatgag ctggcgctgg
     4141 agagaaaatt tttgagttgg tttttaactc gacgtggact tctagtccaa tgactgtttc
     4201 aaaattcatt cgtctttccc tcctaaaata gctggttgtt gtttatggaa ttctgttgct
     4261 tgttcaaacg catatgccgc ttgatacatc gttttttcat caaaataatt accgatgatc
     4321 tgcaatccaa ctggtaatcc ttctgataaa cctgccggta cagacatacc tggaagtcca
     4381 gccaagttga cagggatcgt taagatgtca ctcatgtaca ttgtgattgg atcgttaatg
     4441 ttctcaccga ttccgaatgc aactgttggt gaagatggcc cgatgatcaa gtcataatct
     4501 gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga cttgtcccgc ttttttgaag
     4561 taagcgtcat aataaccagc acttaaggag aatgtaccta acatgatacg gcgttttact
     4621 tcttcaccga atccttctga acgtgagttg acatatacat catctaatga tttcacattt
     4681 tctgagcgat aaccgtagcg gataccatcg aatcgttgca agttggaact tgcttctgat
     4741 gaggcaatga tataataaac agccacaccg tattttgaat gaggcaagct gacttcttcc
     4801 acaacagcac ctaatgaacg gaatgtttct gctgctttca atacagcttc ttttacgcca
     4861 ggggccacac cttcgcctaa gtattcttta ggtaaagcaa tcttcattcc tttgatgtcg
     4921 cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg aagaagtacc gtctttttcg
     4981 tcataaccgc tgatagctgt caatgctaat gcattatctt tcaccgtacg ggtcattggt
     5041 ccgatttggt caagagatga agcaaaggca atcaaaccaa aacgtgagac acggccataa
     5101 gtaggtttca tccctacgat gccgttaaaa gctgctggtt ggcggatact tcccccagta
     5161 tcactaccta aagaaactgg aatttgtcca gctgcaactg ctgctgcaga accaccagaa
     5221 gagccaccag gtacttttgt tgggtcccaa gcattttttg ttttcttgaa ataagaagtt
     5281 tctgtacttc cacccatagc aaattcatcc atgtttagct taccaacagg gatcatatct
     5341 gcttgataga ttttatccat gaccgttgca tcataaattg gttcaaaatt gtaaaggatc
     5401 ttagatgcag cggttgtcaa gatatctttt gttacgatat tatccttgat accaattgga
     5461 ataccagcaa ggacgttgct ttcatcaatc cctttttcat ctaaagcttt tgcctgttcc
     5521 aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct caggctctgt ttctttgatt
     5581 cgttcaaatg ttgcttttgt caaatcttgt acagtgattt ctttcgatac aagcatatga
     5641 tgcagttctt ctagagaacg attgtgtaat tctgtcatta tgcgccagcc tccccattat
     5701 cgatgattgc gggtactttg ataaagccgt cttctgtttc cggcacattt ttcaataatt
     5761 catcgcggtt ccatccagct tgcgcccgat cttgtcgcat cacgtttact gtttcaacca
     5821 cattcgacgt aaatggaaca ccttctgtat ctacttcttc cagcatttca accatatcga
     5881 tgattttacc taattggtcg gtaaaacttg caagttcttc atctgcgaac gcaagttttg
     5941 ctaatttggc aacgtgtttc acttgttctt cactaatcgc catactaccc cttctttcta
     6001 aacgagttaa aaactttgct ctctatttta acacaagagt gccgtgtctg aaattgttta
     6061 ttgaaatttg tctgaaaaaa ctcatttttc attcttctac tgagcgactt ttagccaatg
     6121 catctgccat ttgatcttct gtccaaactt cgattcctaa ttcttgtgct ttggtcagct
     6181 tgctgccagc atcttctcct gctaccacga tatccgtttt tttcgatacg ctaccagtca
     6241 ctttcccgcc aagattttcg atcgtctctt ttgcttcttc acgagtaaat cgtgttagtt
     6301 taccagtaag gactactgtt ttttctttaa aaggagactc gacttcttgc aattgggcat
     6361 ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt taattcattc atcaattcat
     6421 ggacttcttc gttactgaaa taagtgacta cactgtcggc aatgatatca ccgatcgtat
     6481 caagcgcaat aatcgactca tagtcacttt tactcaatgt ttctagatca ccgaaatgtt
     6541 ctgctaaaat ttttgctgct ttcgctccga catgtcggat tcctaacccg aaaatcaacc
     6601 gctcgaccga attatctttg ctgttgtcga ttgctgtcaa aatattattt gctgattttt
     6661 cttttatttt gtccaacgtc aataactctt cttctgtcaa tttgtaaaga tcagcaacat
     6721 ctgctactag ttttttatca tacatctgtt ctagtacgcg cggccccaac ccatcgatat
     6781 tcatcgcatt tcttgaaaca aagtggttca aaccttcttt catttgagca gggcatttag
     6841 gattgatgca ccgtaaggct acttcttcat ccaaatggac aagttcactt ccacaaactg
     6901 gacaatgtgt aggcaattgg tattcttcgc tgtctttagg tcgtttatct aacacgactt
     6961 gcgaaacttc tgggatgatg tctcccgctt tataaatcag aactgtatct ttcaagcgga
     7021 tatccttcaa cttaatataa tcaccgttat gcaggctggc acggcttact gtcgttcctg
     7081 ctactcggac tggtgtcatg atagctgttg gcgttactac ccctgttcga ccgactgtcc
     7141 attcgatgtt ttcgattagc gtttcaactt cttctggcgg aaacttgtaa gctgctgccc
     7201 atctcggtgc tttgacagta aaaccaagtt gatcttgtag cgagaattca ttcactttga
     7261 tgacgattcc atcgatttca tagggcaaat ccacacgttt gtcatgatat tcttcaatgt
     7321 atgaccacac ttcatcaatc gaatgacata gacgtttttc ccgattcgtg tggaatccaa
     7381 ttttttctag ctcttctagc gcatcatact gggtttttgc ttgcatagga ccgaaatcag
     7441 ctactgtgta taaaaaagtg tccagatttc ttttcgctgt gattttagaa tctaactgac
     7501 gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat atcttttccc tctgcttctc
     7561 tgtcttggtt caattggaca aatgaccgtt ttggcatgaa acattcgcct cttacttcga
     7621 tgttcattgg ttcttttaat ttgattggga ccgaccggac agtttttagg ttttcagtga
     7681 tgttttctcc tacagttcca tccccgcgag tcgcacctct cacgaaattt ccatcttcat
     7741 aacgcaaaga aacggaaagt ccatctattt ttagttcaca gcaatagtca actacacgtc
     7801 ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc ttctttgctg aaaacgtcgt
     7861 tcaaactgta aagcggaata tcatgagtga ctttttcaaa accttctaat acttttccgc
     7921 ctactcgttg agtgggagaa tcagacgtga tcaaatcagg atattgttcc tcaattgcta
     7981 ctaattctgc gtattcttta tcataaacat agtcttccac tgttggttta tcttctacgt
     8041 agtattcgcg agaccattgg ttcagtcttg tacgaagttc ttctgctcgt ttagcagctt
     8101 cgtcaatcgt catttcatgt tccatcattt accctccact actaagcttt ttcaattggc
     8161 gcaaaggcag ccaaaagacg ttttactcct tgacttggga aagccacatc tagttctaga
     8221 tccttcgacg tcccgcttac tcggacgact gttcctagac cccatttttt gtgtttgact
     8281 ttatctccag gtttccagct gttattttcc cctccagtag cacttttaga gggaacaacc
     8341 gctgtctctg gctgtttata gctatatgct gtttttttag cgaatacagg tgttttttta
     8401 ggcgtaggac gcatgccttc gatttccagt aactcctgat cgatttcttc tacaaaacga
     8461 gaaggtcggt tatattgcgt ccgtccatat aatgttctgg agaaagcatt tgttaaatac
     8521 aacgcttctt ctgctcgcgt gatccccaca tatgcgagac ggcgttcttc ttccagttcg
     8581 ctttcttcca tcaatgctcg agataaagga aaaaccccct cttctagacc aatcaggaat
     8641 acaactggga attctaatcc tttggctgca tgcaaagtca tcaatgtcac ttgggaagca
     8701 tcttcttcta aattgtcaat atcggaaaca agtgccagat catttaagaa gaccgttagt
     8761 ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt cgaattgttt gtcgaattct
     8821 tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg cttgtgcttc taatgtattt
     8881 tggattttta agtcttctag atatccgcta cgatccaaaa cctctttcgt caattctgtc
     8941 actgtcaagt atgagatcat ctgagtgact tcttggatca tctctccaaa tgcacctaat
     9001 tgctgtcctg cttttccacc gatatttgcc agatccacat tttgcgctgc ttcaagcagt
     9061 ggccattcat gaagagaagc aaaactgcgt agtttttcga tagatcctgg tccgatgcct
     9121 cgtttgggcg aattaacgat acgctcaaaa ctgattgaat cttgaggatt tgccagcaca
     9181 ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt agaacttgtg ccctccaacc
     9241 attttataag gaatatttgc tttcaacaga gtttcttcca ttacacgaga ttgtgcgttc
     9301 gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat tgcttcgaat ctcttcttgc
     9361 atccgatcta cgataaaacg ggtttcgtct cgttcattat ctgcacggta gtaagtgatc
     9421 ttgtttcctt ctttgttttc tgtccacaag ttttttggtt ttcgattgct attgttttca
     9481 atgacttggt ttgctgcgct aaggatgttt ttcgtggagc gatagttttg ttctagcaaa
     9541 attactgcag catctggata atctttttca aaatccaaga tgttctgcat atctgcaccg
     9601 cgccagccgt aaatactttg atctgcatct cccacgacgc acaggttcct aaatctacct
     9661 gccaataaat tgactagcgt atactgtgcg tgatttgtat cttggtactc atctacatga
     9721 atataatgga atttgttttg ataatatgtg agtgaatcag gatgttcttc aaataatcga
     9781 atcgtattca tgatcaggtc gtcaaagtcc atacattgat tatattgaag ctctttttgg
     9841 taagcagcat aacattttgc cgcaatctct tcgtaataac tgccttgcat ttttgcaaaa
     9901 tcctgagggg tctgcaggct gttttttgct tgagagattg ttcctaatat acttcgagga
     9961 tcatattttt tcggatcaat gttcagttct tttagtatcc gtttcatcaa ggtcagctgt
    10021 tcagaagaat cgatgattgt aaagttccgg ttatacccga taaaatcaac atctcttctt
    10081 aaaatacgca cacacatcga gtgaaaagtc gatacccaga catcttcgcc tccagatgcg
    10141 agaatcgcat tgacacgttc tttcatttct tttgcagcct tattggtaaa agtaatcgct
    10201 aggatgttcc aaggattgac ttctttttcc tcaatcaaat aggcaatgcg gtgtgtcagc
    10261 actctcgtct ttccacttcc tgcacctgcc attagcagta atggtccgtc agtgtgaaga
    10321 acagcttctt tctgacgggg gttcatcccg tttaataatt cagctttgga tgacattaac
    10381 aacatccttt cttttatcaa tcttgttaat tatagcattt tcttggcttt cattaaacta
    10441 gctagttcaa aatcatttgt ttctttttat ggatttttcc ttgtcaattc gttgatttcg
    10501 cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa aaggaaaaat caagaaatta
    10561 gataagactc agatatattt tattctaaaa ggaatatttg ttggactgct gacaggaatc
    10621 gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg atttagttcg aagtatctat
    10681 gaaatgagtc gggaacagcc gatttactta attggcctag ctatttgctg catccttgca
    10741 gcatttttcg tgggttatct ggtcaaaaat gaaccggata tcaagggaag cgggattcca
    10801 caagtagagg gacagcttcg cggggaatta tcaatgaact ggttctctgt tttatggaaa
    10861 aagttcattg gcggtgtact ttcagttggt gcgggccttt ttctaggtag agaaggacct
    10921 tctatacagc ttggcgcatc cattggacaa ggagctggac aattgtttcg gagtccatct
    10981 tcagaagaaa agattttaat ttctagcggt gcaagtagcg gacttgcagc agcattcaat
    11041 gctcctatcg caggattact ctttgtttta gaagaaatcc atcactcctt ttctccactt
    11101 gtttggctga catcatttgc ttcagcaatc actgctaact tcgtctctct ttactttttc
    11161 ggcttgagac ctgttttata tctaggtgac cttccttctt tgccgttgaa atattatggt
    11221 tccttagtga tcttaggagt acttctaggc atcttaggtt ttatttatca gaaggtcttg
    11281 ctggctttgc caaaatttta caaccgattg cctttaccag cttattttca cgggttcgtt
    11341 cctttcttgc tgattttgcc aattggttat ttatggccta gtactctagg cggaggcaat
    11401 caaatcgttt tagcttttgg acatgcttcc ttgccaatca ttatgattat cggtttgttt
    11461 gttcttcgat tcgttttttc catgatttct tacggtggtg gtcttccagg ggggattttc
    11521 cttccgatat tgacattggg ggctttaatc ggtagctttt atggaaacat tatggttgat
    11581 ttcgggatgg atccaatcta catcaaggat tttgtcgtta ttgccatggc tggttatttc
    11641 acagcgattg gaaaagcacc gctgacagca attattttag tgacagaaat ggttggctct
    11701 cttaatcatc tgatgccttt aggattggct gctcttgttg cttatatcgt caatgacctt
    11761 ctcggcggta atccaattta cgaatcatta ttggaacgtc tacttggaaa tactcagacc
    11821 gcctcactaa aaggtgcaag gacaactttt gattttccag taacggcaga gagttcttta
    11881 gacggattaa tggtacgaga cttcaattgg cctaaaaata tgctgctaat ctctatacgc
    11941 agaggaaatc aggaaatctt gactcacggt gatacgatca tgaatgtagg agatattttg
    12001 gttatcttga ctgatgaagc aaacttgcca agaatcaagc aggaaatcga ggctaaatct
    12061 tctatcacaa atttgaagca atcattttaa cgaacatgga aaaactattt tacaacaact
    12121 attcatctac actacttgat tctatatgat tctataaaaa aagaagagac tgggaaaaac
    12181 cagcctcttc ttttttattt ctatgcttct gctgctgcag gagcttttct caataaacca
    12241 taaatcacac cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga
    12301 ctcagtaaga atacaacgaa aatcccgcca tgaggagcca gtaatttgat cccgaaagct
    12361 ccgactaatc cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg
    12421 tcagctgctg caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc
    12481 gtcaaaccag catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg
    12541 aaaattgcca atggaggtac cattcctcca gccataacag aagccatcac gacacttccg
    12601 ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt
    12661 ggaccgccta aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc
    12721 gaaccactca tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca
    12781 ttgaccagca acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc
    12841 gttttaattc catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg
    12901 acataaccag ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta
    12961 gctaatgcac cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca
    13021 ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac
    13081 cagctagcag cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata
    13141 aattggtcga tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac
    13201 atgtgtgata cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca
    13261 tttccttctt ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta
    13321 agggctagat tgatcagctc ttctggttta cggataccat cactgacagg acgattgatc
    13381 agttcttttc cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct
    13441 tctgcacggg cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt
    13501 tcgactttga tctccacacc catttctttc gcttttttct tcagagcatc ttctgccatg
    13561 taagtgtgag cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga
    13621 tgagcagaag cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc
    13681 tcttcagctg tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt
    13741 cctacgaaat ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg
    13801 tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag
    13861 gcttcataat ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca
    13921 ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg
    13981 cgggctagaa ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt
    14041 ttttggacca tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc
    14101 gcttctttga ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt
    14161 ttgtgatttc aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag
    14221 aaaaagctgt tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg
    14281 tacggctgta agccccaata aagccggcaa tcattgaatc ccctgcacct actgaatttt
    14341 tcaatgggcg aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac
    14401 cgtctccagc cattgagatc agcacattct gtgccccttc agctagcaat tttttaccat
    14461 acggaatgat ttcttctaca gattggaagg ttgtattgta aagctcagct aattcgtgat
    14521 tgtttggttt gatcaacaat ggtttcttaa caagtgcatt ttttaggtca tcacctgttg
    14581 tatcaatcac gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc
    14641 cttttcgcag gctagccggc gcgcttcctg acaatacgac gatatctcct tgtcctactg
    14701 ttcctaatgc ttgcttcaac tcatcgattt cttcatctga aatagatggg cccaaaccgt
    14761 taatttcagt ttcagtatct gattttagtt tgatattgat tcttgtatct tcttttacgg
    14821 ttgtgaacaa agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga
    14881 accctcctaa aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa
    14941 cattgatccc ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta
    15001 gttttaaccc atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa
    15061 tcattgtatt gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg
    15121 acaacgctcg gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg
    15181 gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg
    15241 tttaacagct gcctcttctg ggtccggagt cgtgaatcct tgttcaagat gggcaccgtt
    15301 cattcccata aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc
    15361 taaaacagca ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa
    15421 atcaactaaa tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg
    15481 cagaaaagga atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc
    15541 attaatggtt tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga
    15601 tttttcactc atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac
    15661 tctttctaga aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga
    15721 agcattaaaa aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc
    15781 taggattttt tgtctacgtt cctctgtaag cattttcacg atcacctcaa tacaaataat
    15841 agcatatttt ttttctactc gcaatataaa tcagtcaaaa acgttcataa aaattataaa
    15901 tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt tttttatttt tttgtctatc
    15961 atcaagccgt tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca
    16021 ggttttcttt ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa
    16081 aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat
    16141 gatgccggaa ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt
    16201 ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg
    16261 ctcacctttc ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt
    16321 aacatcagag agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg
    16381 aacatagatt cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc
    16441 tagatattcc ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg
    16501 atcgataact ttatagcaca tcatgcgcga gcgattctcg tcaacccaag gaaaatacgc
    16561 ctgagcattt agtgttccgc gaacacgcct ttctttataa tctccttgtt gaatatcata
    16621 actttttgtt acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg
    16681 atactggaca gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa
    16741 taaaccgaca ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga
    16801 ttgaaacatt tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca
    16861 tatgacttcg ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg
    16921 tttagggatg atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca
    16981 taaaccttgg taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc
    17041 gttttgttgt ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg
    17101 ttgagagatc tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa
    17161 caccccatga gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt
    17221 gaaaacagag atagacaata cattttttat caataattga ttatcttcca agcgaaaact
    17281 acattcgttc tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat
    17341 tattttttct tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat
    17401 aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt
    17461 ttcttcttca tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat
    17521 tttctcacct ctttaaaatc tttttataat ttaattataa ccaacgataa agaaaaacta
    17581 aaactttatg tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac
    17641 aaagaggtgt ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc
    17701 gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt
    17761 ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc
    17821 aaaaaaagac ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt
    17881 ctttttatga ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg
    17941 taacggaaga aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt
    18001 cgctctcatg cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa
    18061 ttacagggat atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct
    18121 tatgacgcaa atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg
    18181 tctcgatcat ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc
    18241 ccggaacatc gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct
    18301 ttcctggctt accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag
    18361 aagatttccc tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat
    18421 attgttttgg tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc
    18481 tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg
    18541 gcagcctctt ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca
    18601 ccagacaatt cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg
    18661 atatcaaaca tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg
    18721 gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt
    18781 tcggtattat ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca
    18841 gtcaagatcc gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat
    18901 cgtccgataa actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc
    18961 atgacacttt cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg
    19021 cccatacgat tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat
    19081 atgttcgtcg tatcaatctg aataaattct tgatgtggat gtttacgtcc tccctgaggt
    19141 ggcacgcttg caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg
    19201 gaaacgtcac gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca
    19261 atatagatga tccctttttc tgcacgttcc acattgtaat cagcagattg aagcaattta
    19321 agcaaaatgt tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct
    19381 gcgattgcaa aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca
    19441 gaccctgtag gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt
    19501 tcttgtgctt gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt
    19561 tttgcgcgat cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta
    19621 gggacttcag tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta
    19681 cataggtcga tacattcatt acagatgtag acccctggac cagctacgat ctttttcact
    19741 tcttcttggg ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca
    19801 tacatggtct tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca
    19861 gtcttttact atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact
    19921 gacctatacc tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg
    19981 tcacagtccc ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta
    20041 ttacttgatt tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt
    20101 ttcattgtca catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt
    20161 ggcatgttgt atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct
    20221 tgaagatttt cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct
    20281 gcttcacctt cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac
    20341 atttcaggag agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt
    20401 acttcatcat gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata
    20461 cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct
    20521 ttgcgatatt tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg
    20581 aattcgtcat ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag
    20641 actgcttctt ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca
    20701 tctagacttt cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag
    20761 cctgaaccta attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct
    20821 acaaagcctt cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg
    20881 ataactaatt cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct
    20941 gttacttcac ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct
    21001 ggtttcactg ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca
    21061 tcgatttttg gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct
    21121 tctggtaata cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg
    21181 aatacttgac gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact
    21241 ttattaaacg cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa
    21301 acaccatcat tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta
    21361 gaaattcatt ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct
    21421 ttcaaattgt aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt
    21481 ttcactgata aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt
    21541 aaaaaatctt gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct
    21601 acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca
    21661 attgcagatt gcaacgtgaa ttcttcattt aacacggaac taagcagcgc atcttcatta
    21721 ccgtaacgat ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc
    21781 agatcttttg gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacaggatgg
    21841 cattcgttca attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct
    21901 ggcgatacaa taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc
    21961 tcatcaatag acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg
    22021 tggagtgcct tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt
    22081 ttttcaatga cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta
    22141 gcatataaaa aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca
    22201 ttttgaagat actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc
    22261 aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa
    22321 gctcgttgaa ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgct
    22381 actcgcatta aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct
    22441 cttgctcatg ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa
    22501 ataactgaag caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa
    22561 ctgttactgc tttttattgc tcatcagctg ttgacttcct ggttgaatgc tttgatcaga
    22621 ttgatcgtaa caacacttgt attagtgaaa aaatcgccta gatgttgctt tttctgatta
    22681 aagattgctg gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca
    22741 ccttctctaa ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga
    22801 ataccaaaga tcattttccc aatcgtttga ccatgattga atttcgtcaa taaaataaaa
    22861 tacgctagat aaataagtaa ggctgtgaat ccgtagatac tcagataaga atctgaaaaa
    22921 ttaagccaac ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac
    22981 aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac ctgcatagaa atacttagga
    23041 aaatcatttg cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt
    23101 ttctttttga tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg
    23161 gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta
    23221 ttttcatttg aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg
    23281 attccgctgt ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca
    23341 atccctggaa ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg
    23401 aagaatattc tactagttcc gcatcttcaa gctgttgttc ctttcgcata gcttttaaag
    23461 catcttcagg aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat
    23521 aaatccttcc atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga
    23581 cgattgctac aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt
    23641 cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg
    23701 tatcttccac acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac
    23761 caattgagcc ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat
    23821 agtagccacc gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt
    23881 tacgaatcgt atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag
    23941 aatttacttc taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt
    24001 ccataaaatt tttatgattg taaccttcag aagcaaataa acctgaagcc cctcccgcag
    24061 caatcgttcc atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca
    24121 catttgaagc tagttcttca tccccatata accaactgtt caatccctca atttgatcgt
    24181 tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa
    24241 tcagaactgc tgcccaacgt ctcttattca ttctttctcc tttctaatac aagtttttta
    24301 tgacgatcac agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg
    24361 catacccgga aaacgactct tttgtcaatt catcttctac cgtaaactca cgagcttcag
    24421 caacttccaa ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata
    24481 ttttcgtatc atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca
    24541 ttcccagtgt catgatctca atttttgaat ccatttttac tcctcctcta tccgattact
    24601 ttctattgta ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata
    24661 aaaagagcat ttgacaaagc atttgatata taccaattta ttttttattc tttttttgtt
    24721 gacaagaaca ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaagagg
    24781 aggatagtta tacctttt
//
LOCUS       DACDIP010000039        24572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDIP010000039 DACDIP010000000
VERSION     DACDIP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.039"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(151..549)
                     /locus_tag="IV257_001738"
     CDS             complement(151..549)
                     /locus_tag="IV257_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8814843.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(546..1319)
                     /locus_tag="IV257_001739"
     CDS             complement(546..1319)
                     /locus_tag="IV257_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8814844.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1316..1999)
                     /locus_tag="IV257_001740"
     CDS             complement(1316..1999)
                     /locus_tag="IV257_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8814845.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2166..3053)
                     /locus_tag="IV257_001741"
     CDS             complement(2166..3053)
                     /locus_tag="IV257_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8814846.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3050..3919)
                     /locus_tag="IV257_001742"
     CDS             complement(3050..3919)
                     /locus_tag="IV257_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8814847.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4056..5342)
                     /locus_tag="IV257_001743"
     CDS             complement(4056..5342)
                     /locus_tag="IV257_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8814848.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5615..6193
                     /locus_tag="IV257_001744"
     CDS             5615..6193
                     /locus_tag="IV257_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8814849.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6193..7122
                     /locus_tag="IV257_001745"
     CDS             6193..7122
                     /locus_tag="IV257_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8814850.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7119..8855
                     /locus_tag="IV257_001746"
     CDS             7119..8855
                     /locus_tag="IV257_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8814851.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8918..9754)
                     /gene="pheA"
                     /locus_tag="IV257_001747"
     CDS             complement(8918..9754)
                     /gene="pheA"
                     /locus_tag="IV257_001747"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8814852.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9751..10260)
                     /locus_tag="IV257_001748"
     CDS             complement(9751..10260)
                     /locus_tag="IV257_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8814853.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10253..11548)
                     /gene="aroA"
                     /locus_tag="IV257_001749"
     CDS             complement(10253..11548)
                     /gene="aroA"
                     /locus_tag="IV257_001749"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8814854.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11561..12652)
                     /locus_tag="IV257_001750"
     CDS             complement(11561..12652)
                     /locus_tag="IV257_001750"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8814855.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12669..13835)
                     /gene="aroC"
                     /locus_tag="IV257_001751"
     CDS             complement(12669..13835)
                     /gene="aroC"
                     /locus_tag="IV257_001751"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8814856.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13837..14901)
                     /gene="aroB"
                     /locus_tag="IV257_001752"
     CDS             complement(13837..14901)
                     /gene="aroB"
                     /locus_tag="IV257_001752"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8814857.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14934..15956)
                     /gene="aroF"
                     /locus_tag="IV257_001753"
     CDS             complement(14934..15956)
                     /gene="aroF"
                     /locus_tag="IV257_001753"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8814858.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16615..17589)
                     /locus_tag="IV257_001754"
     CDS             complement(16615..17589)
                     /locus_tag="IV257_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8814859.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17570..19453)
                     /locus_tag="IV257_001755"
     CDS             complement(17570..19453)
                     /locus_tag="IV257_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8814860.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19471..21696)
                     /locus_tag="IV257_001756"
     CDS             complement(19471..21696)
                     /locus_tag="IV257_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8814861.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21811..22845
                     /locus_tag="IV257_001757"
     CDS             21811..22845
                     /locus_tag="IV257_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8814862.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22912..23925)
                     /locus_tag="IV257_001758"
     CDS             complement(22912..23925)
                     /locus_tag="IV257_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8814863.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24003..24191)
                     /locus_tag="IV257_001759"
     CDS             complement(24003..24191)
                     /locus_tag="IV257_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8814864.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7060 a         5340 c         4397 g         7775 t
ORIGIN      
        1 catattttga aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca
       61 taagtattca cgagaaaaat gcaaacaaaa aagcggaaat aacgctcatc cgccttcttg
      121 tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt
      181 ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc
      241 aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat
      301 gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac
      361 aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga
      421 acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac
      481 atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag
      541 atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca
      601 tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc
      661 attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt
      721 gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta
      781 tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg
      841 tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat
      901 cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc
      961 ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct
     1021 actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca
     1081 atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa
     1141 aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg
     1201 cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc
     1261 aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt
     1321 ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca
     1381 ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa
     1441 gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa
     1501 tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag
     1561 ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata
     1621 ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata
     1681 tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc
     1741 cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc
     1801 ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc
     1861 cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg
     1921 caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt
     1981 ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa
     2041 agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca
     2101 cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg
     2161 tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat
     2221 acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt
     2281 gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc
     2341 aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc
     2401 gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt
     2461 agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga
     2521 aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg
     2581 attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc
     2641 gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt
     2701 tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc
     2761 accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac
     2821 aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc
     2881 acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat
     2941 aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct
     3001 tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt
     3061 ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg
     3121 aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt
     3181 gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa
     3241 aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat
     3301 ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt
     3361 cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag
     3421 tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc
     3481 agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt
     3541 gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa
     3601 agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa
     3661 taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa
     3721 aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa
     3781 atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa
     3841 taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga
     3901 ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa
     3961 gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga
     4021 tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact
     4081 tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga
     4141 gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt
     4201 tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct
     4261 tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc
     4321 gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct
     4381 gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct
     4441 tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat
     4501 tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact
     4561 gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct
     4621 tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc
     4681 gtgactgtct ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt
     4741 actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca
     4801 tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa
     4861 cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga
     4921 acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct
     4981 tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt
     5041 atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa
     5101 gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt
     5161 tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc
     5221 cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct
     5281 gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc
     5341 atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca
     5401 aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa
     5461 aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa
     5521 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt
     5581 aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga
     5641 tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt
     5701 tcaattaata gggacttttt ccagtccgat gaaagcgctt gatttttctt tggtacatgc
     5761 aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg
     5821 gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt
     5881 tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt
     5941 gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc
     6001 caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat
     6061 tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg
     6121 ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc
     6181 acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg
     6241 gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa
     6301 aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc
     6361 caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat
     6421 cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag
     6481 cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa
     6541 gaacttgaga atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa
     6601 catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg
     6661 aatgaagaac tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat
     6721 ttcgaagatt tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca
     6781 aatgcaaaaa aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat
     6841 tatgagaaag aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat
     6901 ctctcagcca tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc
     6961 cgtatcagta aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt
     7021 gagaaagtcg gctataccga actgggttat ttctccagag tttttaaaga agagacagga
     7081 atgacacctt ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc
     7141 atgaactctt tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg
     7201 tcatttcttt gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat
     7261 cgaatgatgt ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg
     7321 agaatacatt agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc
     7381 cagcccaaca tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc
     7441 tttcaaaaat ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa
     7501 cttttgtcgg aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga
     7561 ttgctcagag agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat
     7621 tgactcttcg cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat
     7681 tgaatcacgt ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt
     7741 ttcaatctgt agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga
     7801 tcatcgcatc aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa
     7861 cagtaggtga aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc
     7921 aggattgtac attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga
     7981 tttttccgat tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta
     8041 tgtatttgga tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag
     8101 aggaagatgg cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag
     8161 ggaatacgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag
     8221 acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt
     8281 ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag
     8341 cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag
     8401 aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta
     8461 tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat
     8521 ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca
     8581 ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg
     8641 aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt
     8701 tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat
     8761 atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc
     8821 ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt
     8881 attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg
     8941 tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga
     9001 gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt
     9061 ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc
     9121 caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga
     9181 cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa
     9241 aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat
     9301 gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat
     9361 acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt
     9421 ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg
     9481 ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga
     9541 cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct
     9601 ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga
     9661 tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga
     9721 atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt
     9781 atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc
     9841 ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc
     9901 taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc
     9961 ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac
    10021 aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga
    10081 ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg
    10141 gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa
    10201 ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat
    10261 gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg
    10321 aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca
    10381 gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg
    10441 gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt
    10501 cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga
    10561 tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca
    10621 gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta
    10681 aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct
    10741 ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt
    10801 ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg
    10861 gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt
    10921 ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt
    10981 ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt
    11041 tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg
    11101 tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc
    11161 gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag
    11221 tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt
    11281 ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct
    11341 tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac
    11401 tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc
    11461 caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta
    11521 atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat
    11581 aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac
    11641 tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag
    11701 aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca
    11761 catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt
    11821 ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga
    11881 tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct
    11941 gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga
    12001 tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt
    12061 ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta
    12121 tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt
    12181 cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat
    12241 tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat
    12301 gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac
    12361 tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat
    12421 aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg
    12481 tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt
    12541 ttgactgact atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg
    12601 ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg
    12661 cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca
    12721 acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca
    12781 ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct
    12841 ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg
    12901 ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg
    12961 cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc
    13021 acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag
    13081 gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg
    13141 tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta
    13201 tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca
    13261 acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca
    13321 ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt
    13381 tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca
    13441 gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa
    13501 tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact
    13561 tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg
    13621 acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt
    13681 tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg
    13741 atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct
    13801 ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt
    13861 gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat
    13921 attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg
    13981 atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc
    14041 tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc
    14101 aatagctact ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg
    14161 gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc
    14221 cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag
    14281 atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc
    14341 tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac
    14401 ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt
    14461 gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt
    14521 cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc
    14581 acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc
    14641 taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac
    14701 gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata
    14761 tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg
    14821 aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt
    14881 atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg
    14941 agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag
    15001 taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg
    15061 atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca
    15121 agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt
    15181 ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga
    15241 atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca
    15301 ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt
    15361 ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata
    15421 tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc
    15481 attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg
    15541 aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg
    15601 gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca
    15661 gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg
    15721 ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct
    15781 actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca
    15841 atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga
    15901 tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt
    15961 tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt
    16021 ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct
    16081 ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta
    16141 cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag
    16201 atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa
    16261 acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta
    16321 atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta
    16381 agaattgtac ctagtataga cataaaaaaa tcgactgtca atcattttta tgaaactttt
    16441 tttaattttc tgatagttaa ctttgttttt cagaaaaata acaattggtt ttattgaaga
    16501 aaatagtcat gaacaaaagt atctgagaac ttttttactc tcttgttcat gacatataga
    16561 ataaagcagc tttgtcttgt aaactccgtt tttcaaataa taatctaaag attgctactg
    16621 aataataaag ccgatttcat ggtcctcttc gctgctgatt cggtaaggtg actcaacatc
    16681 cgctccccag ctattgatac ccccgactcc tcttactttt gcaaaaatcg taagaaccgt
    16741 tcgtctttct ggcggtagtt cttctatatg tgttgcctgt tccagttctt ctgctttgaa
    16801 tggtaaagcg gaaaaagcaa atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc
    16861 ttgatcttta tccgtattat ccaacgtttg atttcttgtt agttctaccc atttcgtttc
    16921 catatgcatg ccgcattctt ggggaacaag gtatggggaa acggggagtc ctgccacttt
    16981 ataaatacct tcgacaccac cagccatccg atccggatat gtttcccctg ataggccttc
    17041 atactcaaaa aattttgctt tcgttggcat aatgaacctt aaacccaatg cgggtaattc
    17101 cggtaatcct ttttgtccat aataatgcat attgacaagc attttaccct ctccagcgat
    17161 ttggtaggaa attgtcactt gggtttttgg tactgtgttg atttccagtg taaaaatgat
    17221 ttcaaccgtt tcagcgtatt cattacttga atattgatta tttactggag caatcggcaa
    17281 atcgatcttt ttattgtcca ccagtacttc tatttctttg acttttggat acaatccggc
    17341 tcctaaccac ataccggatt taaatccaaa tccgctacct ctgtcattat cggtcgttgc
    17401 tcgccaaaaa actggaagtg tttcccggta tagccattct tttccgtttt tattgagcga
    17461 ttccagtccg cctcttgtat aagaaaaaat ataatgcgta ttccccgatt tgacacctaa
    17521 tgtacagtcg ccgaagacga ttgcaacttt ttccgtatcc atatgacttt tatttactgt
    17581 atttttcata ataatatctt acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa
    17641 cgatcccatt ccctgagaat tcatagtcac ttggtttatc gtcaaagtca ccgccatagc
    17701 gcatcacttc tctgcctgtg attggatcag tcacttggat cgcctgatct ttaaaatccc
    17761 atatatagcc tccttggtac atttcataac gatcaagcaa cgtcatgtaa gaatccattc
    17821 cacctaacga attccccatg tcatgcatat attcacataa gatgaacggt ttttccgctt
    17881 tattttcaag gtagtcaatg atctcttctg gtggcgcata catgcagctt tttacatctg
    17941 aaatcgaatc ttcgaatgct ggatttggaa aattcccttc ataatggaca agtcgatcgg
    18001 ggtctacttt tttgaagtaa tcgttcattt gtcgaatgtt ttcttccgca taagattcat
    18061 ttccaagaga ccaaaaaaga atacttggat ggttttttaa tgtttcgaaa ttcgtttttg
    18121 ctcgatcaag aacagccgcc tgccactgag gtactgcacc tggtacgttg taagaaggct
    18181 caaatgcgcc cattttttgc catgagccat gggattcgag gttcgtttcc gccatcacat
    18241 ggattcctgc ttggtcgcat aaataatacc aagtgatctg gttggggtaa tgagatgtcc
    18301 gtacgccgtt gatctttgca ttttttatga tagccaaatc tttttccata tcttctatag
    18361 tgatggcgcg tccttttttc ggatgccact catgtcgatt taccccgttg ataatcatcc
    18421 gttttccatt gaagtagatg attttatctt tgatttcgat tttacgaaaa ccgaaggaat
    18481 agtcaatata ctccgttact cttttttctt cgtctatcgt ctccaattca agcttatata
    18541 aattgggctg aaaatgcccc cacagtgcca cattttcaaa tccatcgatg cgaatcgtca
    18601 tttgctcttt taacggatat tgctgttgat agatttcctt cccatcagga tctagtatcg
    18661 ctacattcag ctgactgttt ttggctactt gctcaaaaga caaatctact tcaagtttgc
    18721 cattttgaca tttgtcatct aagcgaggtt tgatccaaag atcttctaaa tggttttgtc
    18781 ctcgagcaac taagaagaca tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt
    18841 ccaagaatgc agctgtactt cttttgtaaa cctccacagc tagaatattt tcttcttcca
    18901 aataaggtgt taaatcaaat tcagacggag tgaacgaatc ttcggcatac ccgataaaat
    18961 gaccattcaa ccaaataaac attgcttgtt ctacaccagc aaaatgaatc gagatttgtt
    19021 ttccctttag accttcttct aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt
    19081 tagcagctga aaagatccct tccgcctctt tctcagggct tgtcgtgaaa gggggacggc
    19141 gaaatacatg tccctcccac ggatacatcg tattgatgta atggatcttg tcataatttg
    19201 ctaattcaat atgactagga acagtgattt catcaaaaga acaggcatca aaatcttttt
    19261 tataaaatgt ttttggtcgt accgaaggat ttggcgaata acaaaaacgc catgatccat
    19321 ttaaagactg tttaagggag gatgtggttt ctacttcttc ccttgttgca taacaaacat
    19381 gatcgctatg tgcatttgat tggttcactc taaaaacttg tggatcatct aaccatttta
    19441 tatttgcttt catacttttt tccttctctc tcataacttt ttgaaatgaa gcagttctgt
    19501 ttggaaatca ccttgaaaat cggggaagta aagtccaact gtcatcaatt cggcccccga
    19561 aaatatccgc ttcgaggtct gttcctgata aagtgcctca tcatctaacc ccatcagata
    19621 tacttcatgg aacgctggtt gagctgaagc tagtatcctc cctaaaaaca caatagcttc
    19681 atcttgttta ggagacacaa acatccatgc ggcaatattt tcttcaaaag gactcgccaa
    19741 acgataataa tggccatact ggatcaccgg acgaatcgtt tgataggata taatttggtc
    19801 ttttacttgc tttttttctt caggagacag ctttgtcaaa tccagttcat aaccaaaaac
    19861 agcgctcata gctgtttctc ctcttgtttt caacgaagta gatctgtcgg tctgatgatt
    19921 tggtacagct gatacatgag ctgtgatgga cgaaatcgga taagcaaggc ttgttccata
    19981 ttggattttc atacgctcca ctgcatccgt gttatcactt gtccatgact gcggcatata
    20041 ataaataaac ccggcatcaa accgtccacc tccaccagaa cagccttccc ataagatttc
    20101 cggataatca gttgtcaaat gttcaagtag ttcataaagc ccgagcatat aacgatgcgc
    20161 tacttctcct tgtctctgtg ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt
    20221 catatcccat ttgatgtagt caagaggaat cgtatctaaa atctttctca tttgtttctc
    20281 aatcgtctgc cgaacatcta accttgtaaa atccagtaca tgttgtgatc tggaagcaga
    20341 aggcattcta cctggctcct gcataagaaa atcaggatgt gttcgataca gttcagaatc
    20401 aaccgagatc atttcaggtt caaaccataa accaaatttc aatcctttgc tatgagcata
    20461 atctgctatt tcacgtaatc catttgtcaa cttgccttca tactcgaacc agtctcctaa
    20521 tgaagaattg tccgcatctc tttttccaaa ccagccatca tctaatacga acatttcgat
    20581 gcctagctcc tttgcttcat caatgatcgc cttgattttt tcactattga agtcaaagta
    20641 agtggcttcc cagttattga ccagaatcgg ccgttcagca aattgatgac gacctcttgc
    20701 gattctttca cgtagcaact catggtggat ctgactcatt ttgttcaatc cctgacttga
    20761 gtaagaaagg atcatttcag gtgtttgaaa agactggcct gcattcaatt cccacgaaaa
    20821 acgatggctg ttgatcccac cgacgactct caattgattg atctgatctt tttcaagttc
    20881 gaaagaatgg ttgccggaat aaacaaattg aagtccgatc gcttctccag tattttctgt
    20941 cgtatgatga tgaactaaag caataaagtt attcatatgg tgactgctcg ttcctcgacg
    21001 gctttcaaat acttttgttc cagataaaac agattgccgt gaaatctgtc tttcattgac
    21061 atgtgctcct ggcaaagcaa tgacttcatc aaatcgccta gtatgggcaa agtctagctg
    21121 gaaactagcc gctttttcta gtttgattgt ttctccggtt tcatttctta tctttacaga
    21181 tcgtgtgatc actgctctat cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt
    21241 ttctctatcc ttaagtgaaa tcaccaacgt ttgtgcctct gacttgtcca tcacataagt
    21301 ttgaggcaag ccttcgatct ctggcttccc atctgtaata taatggctgt catatctcag
    21361 atccagtaag tctgatccat tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat
    21421 gctgccatga gaagagtatt cttgcggtag tgtatctttc gacagagacc ggtcaggatt
    21481 taagggaaca tttcctgaaa atccacgatc tcttcgggga taatttttat tgtctttgta
    21541 ctgcttgact ggtttgccaa aataaatatg cgccaataca ttgctttctt ctatctttat
    21601 cagataagaa agaaatgtat ttcgtaaatg gaagactcgg ctcttttcat caaataagat
    21661 gaaattttta tctgtttttc tatgcgtaat atccatcatt tcactcccaa caaaacggaa
    21721 accgtttact tttttttata attcctgtat actgtttact aaacaatttt gtcaataaaa
    21781 attttttatt tttttgagga gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag
    21841 gcaaatgtct ctcctgctac agtttcccgt gtgttgaata acgatcagac gatccatgtg
    21901 gctcagacta ctcggcaacg gatagtagag gcagctgctg cgttggatta tgacatcagt
    21961 actcgtaaat atatcaaaaa gagaatgcct tcgattggtg tgatctctac gatcggcaaa
    22021 gcgagcgaag aacaggatat ctattacaaa catttgcgag tagggatgga agaagaagct
    22081 cgacgtctgc atttagggat gaatcgaatc tataatctat ctgataatcc taaacaatgg
    22141 gaagatctag atcagttagg tgcggtgatc gttgttggga ctgttacgaa agaatcgatt
    22201 accgccttgt taaaacaaaa catgcaaata gtcgtcgtgg ataaccctga catccagcaa
    22261 gaggtcgata tggtgtatgc agatcttgaa cggatgacta aaaccgtact ggaattgttc
    22321 ttagaaaatg gtcataaaag aatagcctat atcgggggat ataaagtcga tatggatgaa
    22381 ttcggtggga agcagatttc tataaatgaa aagaggatgc gtgcctatca gcattttatg
    22441 atcgatcatc acttagaaaa atatatgtct tatcatctag gtggctggac agaaaaacaa
    22501 ggtgaggtac ttgcgactaa tctattagaa aatgaatccc ctcttcctac tgccattctt
    22561 gtcgggagcg atccaatggc aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa
    22621 attggagaag agattgttgt agcaagcttt gataatatcg agacaatcgc aaacctctcc
    22681 cctggcttga ccagtgttca aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg
    22741 gcattggagc gaattgacgg tatacgggac gaaccaatca ttttgactta tcctgcacga
    22801 ttgattgtta gggaaagttt tatcccaaga acaaaaaggt attgaaagag aggttgtgaa
    22861 aagccgcttc gtttcttcat actcaaaacg aaacggcttt ttcacaacct cttacttata
    22921 cttattcaca aaatccaatg tctgctggta acaattttgc gattctttta gcgtgtaatc
    22981 aaattggtat tcatgggtaa tctctttttt tgtatctgta taaaacaagg attgcacggg
    23041 tacctttaat tctgtcaacc gattttctaa tgcttgacct tgctcctgga atgagtatgc
    23101 atttccgtca gttatatacg ttggcggaaa ttccttagaa acatggtcag ccacactagc
    23161 ttctttcaat tgctcactcg ttttccaatc ttttgttcca atcaacgacc acgcaacggt
    23221 tttgacgaaa aacttcataa atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc
    23281 aacaggtccg caataagaaa tggttccttt cagactatct tttggaagta gctgtttgat
    23341 tttcatttgt ttagcatacg catcattcgt ttgtatagct gcatattgca atgcgatttg
    23401 tgaacctgca ctatctccac caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt
    23461 ttcttcagct aataaatctt tgatcagttc attcacttga agaacttgat tagggtattc
    23521 tgaatcaggc gctaattcat aattcatcgc aaccactgcg atgttggcgt ctgaaactaa
    23581 ttttgtcgca aattctttga cacccgattt atctccgccg acgaatcctc caccatgtac
    23641 ccaaaataga acaggaacag gccccttgct gttttttgga tagtagatgt cataagtatt
    23701 ctctttatac ttggactcat acgtcaaatc tgtttttttc gccacgtttt tttctgcttg
    23761 atcgaacgtt tttttatcag tgatttgaac agtatttgaa aacatatggt tgatcaagaa
    23821 tgctccaggt ctgggggaaa gctggaaggc aagaaagatt gcaaacgcga tcccaagaat
    23881 gattccgaag aaccatccga taaatttaag aattttcttt ttcataaggc tccctcagta
    23941 gttagtatta agcattctga ttataccata gtgttatttc ctatcaagct ctcctagatg
    24001 atttaatgat tttccccaaa cttcttctgc aatgcttttg acaaaagaaa ttttttccat
    24061 tgttcttcaa acaaaacatt ttcttcttct gttgaagcaa atccacattg aggacttaag
    24121 cacaaacgat ccaaaggcag atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg
    24181 tgctcttcca tttcgccatt tttcgttaca gtcaaaatct aagacaacta atttatcttc
    24241 agagactttc tctaaaagaa caaaactccc agcacgttca ctatcgtatt ctaaataata
    24301 ggcacggaca ttttcttgag taaaataatg ggtctgctac gtcgtcatcc cccctgatgc
    24361 cgccaaaatc gaataataat ttccgcgtca aacatgtata tttagtgttg aatcatttga
    24421 gaatatgaaa ataaaaatct ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat
    24481 ctgagacaga atgacgttca ttctgaccca gatttgattt tgcacaactc aacagtcctt
    24541 attggcaaat cgccaaaata atgtagacta ac
//
LOCUS       DACDIP010000040        23676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDIP010000040 DACDIP010000000
VERSION     DACDIP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.040"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..147
                     /locus_tag="IV257_001760"
     CDS             <1..147
                     /locus_tag="IV257_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8814865.1"
                     /translation="ELDRQTNWSKKLHQDRFKFVEKYKEILEEYLRRTANDKKTRSIQ
                     HGFI"
     gene            107..352
                     /locus_tag="IV257_001761"
     CDS             107..352
                     /locus_tag="IV257_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814866.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            349..768
                     /locus_tag="IV257_001762"
     CDS             349..768
                     /locus_tag="IV257_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8814867.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            765..1322
                     /locus_tag="IV257_001763"
     CDS             765..1322
                     /locus_tag="IV257_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8814868.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1319..1615
                     /locus_tag="IV257_001764"
     CDS             1319..1615
                     /locus_tag="IV257_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814869.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1692..2105
                     /locus_tag="IV257_001765"
     CDS             1692..2105
                     /locus_tag="IV257_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8814870.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(2563..2838)
                     /locus_tag="IV257_001766"
     CDS             complement(2563..2838)
                     /locus_tag="IV257_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814871.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            3292..3498
                     /locus_tag="IV257_001767"
     CDS             3292..3498
                     /locus_tag="IV257_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814872.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            3694..3861
                     /locus_tag="IV257_001768"
     CDS             3694..3861
                     /locus_tag="IV257_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8814873.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            3887..4231
                     /locus_tag="IV257_001769"
     CDS             3887..4231
                     /locus_tag="IV257_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8814874.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            4236..4517
                     /locus_tag="IV257_001770"
     CDS             4236..4517
                     /locus_tag="IV257_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814875.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            4620..4934
                     /locus_tag="IV257_001771"
     CDS             4620..4934
                     /locus_tag="IV257_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8814876.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            4912..6606
                     /locus_tag="IV257_001772"
     CDS             4912..6606
                     /locus_tag="IV257_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8814877.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            6626..7804
                     /locus_tag="IV257_001773"
     CDS             6626..7804
                     /locus_tag="IV257_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8814878.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            7767..8453
                     /locus_tag="IV257_001774"
     CDS             7767..8453
                     /locus_tag="IV257_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8814879.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            8453..9613
                     /locus_tag="IV257_001775"
     CDS             8453..9613
                     /locus_tag="IV257_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8814880.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            9623..10498
                     /locus_tag="IV257_001776"
     CDS             9623..10498
                     /locus_tag="IV257_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814881.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            10495..10806
                     /locus_tag="IV257_001777"
     CDS             10495..10806
                     /locus_tag="IV257_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814882.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            10796..11149
                     /locus_tag="IV257_001778"
     CDS             10796..11149
                     /locus_tag="IV257_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8814883.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            11139..11540
                     /locus_tag="IV257_001779"
     CDS             11139..11540
                     /locus_tag="IV257_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814884.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            11533..11937
                     /locus_tag="IV257_001780"
     CDS             11533..11937
                     /locus_tag="IV257_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814885.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            11949..12557
                     /locus_tag="IV257_001781"
     CDS             11949..12557
                     /locus_tag="IV257_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8814886.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            12577..12939
                     /locus_tag="IV257_001782"
     CDS             12577..12939
                     /locus_tag="IV257_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814887.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            12942..13124
                     /locus_tag="IV257_001783"
     CDS             12942..13124
                     /locus_tag="IV257_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814888.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            13141..16572
                     /locus_tag="IV257_001784"
     CDS             13141..16572
                     /locus_tag="IV257_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8814889.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIDNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            16623..17360
                     /locus_tag="IV257_001785"
     CDS             16623..17360
                     /locus_tag="IV257_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8814890.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            17370..19661
                     /locus_tag="IV257_001786"
     CDS             17370..19661
                     /locus_tag="IV257_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814891.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            19685..21811
                     /locus_tag="IV257_001787"
     CDS             19685..21811
                     /locus_tag="IV257_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814892.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            21974..22420
                     /locus_tag="IV257_001788"
     CDS             21974..22420
                     /locus_tag="IV257_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814893.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            22422..22559
                     /locus_tag="IV257_001789"
     CDS             22422..22559
                     /locus_tag="IV257_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8814894.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            22597..22890
                     /locus_tag="IV257_001790"
     CDS             22597..22890
                     /locus_tag="IV257_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814895.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            22887..23111
                     /locus_tag="IV257_001791"
     CDS             22887..23111
                     /locus_tag="IV257_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8814896.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            23108..>23676
                     /locus_tag="IV257_001792"
     CDS             23108..>23676
                     /locus_tag="IV257_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8814897.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT         8340 a         3564 c         4996 g         6776 t
ORIGIN      
        1 gagctggatc ggcaaacaaa ttggagcaag aaacttcatc aagatcgttt caaatttgta
       61 gagaagtata aagaaatatt agaagagtat ttgaggagga cagcaaatga taaaaaaact
      121 cgttcaattc agcatggatt tatatgatat cgaatcagga gcaacactat ctgtggaatc
      181 ggaccatcta atcataaatt ttggtggaaa gcgccagatt attttgtggg tagttgatga
      241 tgtactgttt ccagaaattg ttcatgattt cgaagaatca aaagcggttg agtttgaaat
      301 agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag gacagcgaat gataccgaag
      361 tttagagcgt gggataaacg aaagaacgta atgagagatg tagccgtctt gcattttact
      421 aaaaacggca aaacaaactt tattgaatat tggataaatc ctaccgaatt gaaatcatat
      481 cacgtgcgaa acatcgacct catgcaatcc acaggaatga aagataagaa tggtgtggat
      541 atatttgaag gggatatagt attagtcagc gtgcgaaatg gcttcgatta cttagataat
      601 aaagtctgta ttgtcaaaaa ttcaatagat tattccggat tagtttgtgc cactgttgat
      661 gaagacttag agtatcaaat ttttaacaca gagctgtttg aagaatacac gtacgaagtc
      721 accggaaata tatacgagaa tagcgagtta ttggaggaac agcgatgaat aaacaggaag
      781 caatcaaaaa gttagaaagt attaaagcga taggaaatga tgcaatagct gcttgctata
      841 acgagagtat aaattcaggt attacgttaa tgaaaaaaat agacgaaccg cagaaaccag
      901 ttattccaca actcgtggcc ggttggcttg agaaatctac ggaccctttt acaaaagctg
      961 aaaaaatagc gtatttaatc aaatctaaag atggtgattc atattatttc tgtgattggt
     1021 ttgtacgaga tggcatagtg acgcaagagc aaggagaaga attactcgct tgggcaacaa
     1081 gacaatcata tgaaacacta ttgagcctat acaacggcta cgaggttgag aaagagccgt
     1141 tatgggcaat aaagaatgcc gatggaaact atcttactaa atgtgcttta tggggaaaag
     1201 atggagtaaa ttatagtttt gaatgcaatc catctcatcg attgcttttc actgataaag
     1261 caacagcgga tgctgcagca ttgttggtga atggaacagt ggaagaggtg gtagaaagat
     1321 gaaactaaaa gacggatttt acgctagcag tcacggtatc ggcggtttaa tgctagatat
     1381 gccgacaaag aaccctaaaa cacgcaagaa atcaaaattc aaagtcggtg acatggtccg
     1441 ctgtgaagca gaagagttcg tttatccgtt cagaggatac gttaaaaaga tactgtcaaa
     1501 ctcagcaatc attcgtattg aaaatacaat gaagtgtgat cgttcgactg caaaacacaa
     1561 gcattattta gctgtagcga gattagttga catggaagta atcaagagca aataaaaaaa
     1621 gccggatcgc tccgactgat tcaataaatc caacacattt attatatcac ataaaggagc
     1681 ggtttgactt gatgcaattg ttacgagagg tagatttcaa acagacaaga tgtaatgcga
     1741 gagatgtgct gaagaacttt cggcgtttgg agcggatggc aggtcgctct ttgatagata
     1801 ttaagtcgcc gattataacg gatatgccga aggcaccgaa gcatggcaat aaggcagaag
     1861 acgcgattat tcagatgatg gatatagaaa cggagagaga tgcgattcta gcggctttga
     1921 tggctcttag tctgattagt cgtcagatac tctactacag cttctgtgtg ccagatagct
     1981 tctcaaacta cagaattagc cgtgaagtgg gttattcaga aagaagtata caacggatga
     2041 agtcggaagc tctaatagag tttgcagaag catataaaca cggaaaaata attgcttata
     2101 aataatttgg cggttttttg gcggaatgat ggcggttttt agacatttac cagtgttatt
     2161 atggtagtgt cgaaagatta ggaaacagga tcgacaaaat aaacgaaggg aggcaatctc
     2221 cctcatcgtt ttaaacttct tcttagatag acagcaacta attatgaaga aaaggatgtg
     2281 aattcaactc ctcctaaatt cttcatcggt tgctgtttat tttgattaga cagcagcact
     2341 cacaaaacta aacatgttct tgtcgtcttt caactgctgc tgtctattaa tttatgtatt
     2401 ggagggaaga aaaatgaaat tatcagtaga aggcactcca gaagaaatac aagaattgct
     2461 ccaagctatt ggtagtagca aggagcaaaa ggtaagtgtt tctaattcag atattgacgg
     2521 tatttgcaat tgtttaaaca gagaaaaaat atctaatcct tactaagaga aaatatgttc
     2581 gttaatgaat ttccaatttt cctctgtagc tctaccttga taattttttt gcagttcaca
     2641 gatgaataag gaatcgttat tatcaagtac tgtatttaat ttttcacaca tttgatttgg
     2701 attcaaatta gttttaacta accaaaaaga tttttgtagc tttatatatg ccccaaattc
     2761 ttttattatt tcaaatactt tatcatactt ttgccctggg ttattaagat cataactaat
     2821 catgtagggt ttatacatgt tgtcttgcac atccttgttt tatatttcag cggaccactc
     2881 gctgataaat aaaattatac gcttagtatt tattttcaca atattaattt atcgtaataa
     2941 cttttgagta aatagaacaa aaaacctgca ctagtttccg ctagtacagg cagtgactat
     3001 atcggttgat aatctagcat atattaaaaa taattgcaag aaaagaaatt tatagtggtt
     3061 tataccaaat tatcaaagca atgttatttt gttgctgtct attgtttttt aattattcac
     3121 acgataacta aaggtgggtg aagagaaatg attccattaa taatttcaat ttttgcgctc
     3181 tgtcttaatg tctatatgat tggatttaaa aatgggcaaa ataaaaaata gtagcagcca
     3241 agaataattt tatagtgtca ctgtggcgga aagggtagac gctaagcatg tgtgctaggt
     3301 caatgcttcg gcaaccatgc aaggttcgat tccttgccag cgacattaaa tgcctatgac
     3361 ggttacgact accgaaaaaa gatcgttaag aagctatacg gtgctacgta cggcaatgta
     3421 gtaagtgtgc tatctgtaca ccaccaagct tcggtcactg tggcggaagt agaagacgca
     3481 gcggtaaatg gcgagtagcc tcgtgagagc ctggtaagtt ctcgtgagtg gtgcaatccc
     3541 actccagcga ctttaagcaa ccgaggcatc ggcggtttaa aaatataggg gtgcgcaatt
     3601 tcgtacgcgt tttgtgcatc gtgcaagtta ctattacata ttagatcact ctttgagtgg
     3661 tctttttatt tttgcacaaa ggaggaaaca acaatgtata gaccacaata cttagaacag
     3721 aagtatgaag taatcactgt tcataaaggt aatggcgaaa aagtatatga gtatagaaga
     3781 ccaataaaga gcgatacata taaacgaaag gaaaacaatg aagttattcc attgtatggc
     3841 aaaagaatag ctaagcatta aataagattg cgaaaggaga cggaacatga ccgaggaatt
     3901 ctatagatgg ctattacagt tgacaagaga agatcgtttg gttaagttct atcagtctcc
     3961 taaatggcgc aggcttagag agaaagcgat gaaacgagat cactatgaat gccaagagtg
     4021 tagaagacta ggtaagtatc atagagtaga gaacgttcat catataaagg aagtcaagga
     4081 tagacctgac ttagctttag atttagataa tcttatttgt ttatgtgttg aacatcataa
     4141 tgaagttcat ggcagatatc ttacagcatt agataaacaa gagaagaaga tagaaagctt
     4201 tgctaacttc gatgcaagtg aaaggtggta agtgcatgat catcaatgat aatggcagag
     4261 agtatgatac agaaaagatt gaagagtatt catcttatac tcagggatta attaaacgtt
     4321 tgatatacgt tcgctatgta ggtattaggg atctgttatc agataactgt tgtagtaaat
     4381 acaaagtgaa tcaagtaaga gaagcgttga ataaagataa taacgtcgaa agaataaaaa
     4441 atgtttttgg atatagtatt gaagagatta attattacat tgacttcgct gaagctttca
     4501 ttccgatggt gagataaccc ccccttaaaa taaatcgcaa attttttggg ggtgatgaaa
     4561 cggagggggc tgtcaggaaa agagattttt tcgaacttta tcatgaaagg agggctaaaa
     4621 tgtttaaaaa cgaattgtct caaaatcgct acagagaaaa attacgccgc tctttaataa
     4681 gccaattgga aagtcagaaa acaaatattg agccattctt agataatgtt gatcgttata
     4741 tcagtttatg ggaaacggcg atatcactgg aagaagatat atccgagaac ggcattagac
     4801 tggagaatgg taaaaagaat gaatcagtag cgttgcttgt ttctgtcaac aaacaaatgg
     4861 gattgatgtt ggataaactt gccattactc ctgaattggt aggtgaagca aatgaatcaa
     4921 ttcctgagtt ataagcatat tgaaaattgg ttcaaagcta tagaagaagg cactatcaag
     4981 gtatgcaaag agcaattatt gctaaaaaat tatctagaag aaagagtctt tactagagaa
     5041 gatatttact tcgataagca gatggtagag gattcaatca atataccagc acaatacttt
     5101 ccattcgaat taattccgtg ggaaaaattt ctacaatgtt ttatttatgg tgttcgatgg
     5161 aaaaaagata aaacactagt gttcaataga tatctttcat taatgggacg tggtaatggt
     5221 aaaactggtt ttgcttcttg gaacaacttc tttctactaa ccgctaaaca cggtattaaa
     5281 aattatgata ttgatatcta tgccaataat gaaagccaag caaagactag ttttgatgat
     5341 gtatttaaag taattaaaga tcatcctgat ttagataaaa aagtatttaa agctacgaag
     5401 gaagttattc aaaatatcgc tacaaacagc aaacttcgtt ataacacggc aaatgctaga
     5461 acaaaagatg ggaagcgacc aggtgcaaac cgctttgatg aaattcacga aaatgaagat
     5521 tattcaatga taaatgtggc tacttctggt ggtggtaaaa ttcgagatta tagagaattt
     5581 tatgatacaa ctaatggtca tgttcgtggt ggtccgcttg atgacattat agaagaatca
     5641 aaaatgattc tttctggaga acttggaatt gacaaggatg gagcagaatt ttctagtttg
     5701 tttccattta tttgtcgctt ggataacgat aatgaagttg atgatcccga catgtgggaa
     5761 aaagcttgtc caactattaa ttacaatgca gatttaaaac ggaaaatgtt tcaagaatac
     5821 tctcaaatgc aacgtaatgc tggtttaaga cttacgttca tgaccaaacg aatgaacaga
     5881 cctatggaag atacacgatt tgctgttgct tcatatgatg atgttctgca tacgaaagaa
     5941 aaagaatttc ctgaaaaaat ggatgaagtg ataggaacag tcgattttgc tgatagacga
     6001 gattttgcca gcgttgggtt gctaggaaaa tacgataaag atgtttattt tacacaacat
     6061 acttttatcc acgaatcagc ccttcgatta caaaacatca aacgagaggt tatagatatt
     6121 tctatagatc aaggaaaatc acagatcgtt catggaaaaa atatagaagc tgattatatt
     6181 gtaggttggt ttcttgaaat gagtaataaa tattatatta aaaaaatcgc tatggatatg
     6241 taccgtgcaa aaatattgaa gcccgcttta gaagaagcag gttttactgt ggaaattgtt
     6301 cgaagcggat ctgttacaca tggtatgtta aaagatctgg ttgatgacct ttttattaat
     6361 caacgtttat tttttggtga cgatgcgatt atgcgttggt attgcatgaa tgtatatgaa
     6421 gagcatattt ctaatggaaa tatacgctat gaaaaaatag aacctgaaac tagaaaaacg
     6481 gatggctttt tttcattcct tcatggtttg aattttttag atgatattta tgattctgct
     6541 cctgtaacag tcacaaatag ctcagtagaa aatacaggaa ctggatttac tcctctagta
     6601 ttctaacttg aaaggaggtg agaaagtggg gatttttcaa aaggcggtag gatacttcac
     6661 aaaaaaagca acggttcctt tagaagaata cttttgtaaa ttgcaagttg attttgtgta
     6721 tcgaaaattt gcgattgaaa cttgtattga tttgattgca aatgcgatga gtaaagcgga
     6781 attcaagtca tatgaagatg gaaaaaataa aaagaatgat ctttactata ggctgaatgt
     6841 agctcctaat aagaaaaata atgcaacaga atttagaaaa aaactgatca ggagattaat
     6901 attctacaat gaagtattga tcgtttctcc gtctaataat tctagcgaaa tatttattgc
     6961 ggatagttgg gatgtcacag aatatgcatt gaaagatgat gtgttttctc aagtgcaaat
     7021 taacaacata gtccttgata gagaatttct agaaagtgat gttatctata taaaatacgc
     7081 agatcaacaa attaggcaac tagtcgatgc gtattatcaa gcgtatggga aactcatttc
     7141 tagtgccatg aatgtttaca agcgctctaa cgctcgtaga tacgtactga aagggaattt
     7201 attccgaccg caagacaata caacacaaga tcaaatcaat aaaatgatga catcacaatt
     7261 taaggctttt atggaagctg ataatgcagg tgcggtattt caattacaaa atgagtacac
     7321 attagaagat ttcagcggaa acttccaaag caattcaaga gatataaaaa acttaataga
     7381 cgacatcttt gagatgacag cagcagcgtt tcacgttccg aaaaacctac taaagggaga
     7441 catgagtggg ttatcggatc aagtggacgc ttttttaatg ttcgaaatca taccgattgc
     7501 tgaacttatt caggatgcgt ttaacgctag tctctatgaa gtagaagaat acttgtcagg
     7561 gaattttgta cgtgtggata caactatgat caagattact agcttcaaag atttggttga
     7621 cgctattgat gtaggcatta gaaatggagt atttacaatc aacgaaggaa gagaacgcgt
     7681 tggaaatgat cgctctgata aggcgatggc agatgaaata tttataacta aaaacaatca
     7741 acaagtatcg aaaggaggtg aggcgaatga cgacaatgaa aacatttcta gcagtaaaga
     7801 atgaaggcgc agtaccgcaa atttttattc agggatttat tggttctagt tggttctttg
     7861 aagggaatac tgacaaggga atcaaaaata ttttggatag tctaggtgat caagaagaaa
     7921 ttgaagtagt aattaattca aacggtggag acgtatttca agggattgct attgggaact
     7981 tacttaagtc aaataaagca aaagttaacg ttgtgattaa cggcttagcc gctagtgctg
     8041 cttcaattat cgcaatggct ggcgatacta taaaaatcta caacaatgca caattgatga
     8101 ttcaccgcgc ttccacatac ggagaaggaa atgtcgatga cttccgtacg attgctgacc
     8161 aactggaatc aattgataaa tcggtaaagg cttcatataa aacacgattc aatggcacag
     8221 atgaagcatt gcaagaactt cttgaaaaag aatcgtttat ggatgcagaa acagctttga
     8281 gttatggatt ggtcgatgaa attatcgatg cagaaaatag ctcaggtact gaagctaaaa
     8341 aagaacaaag cgttgaagaa attttgaatg acgttaaaga aaaaagagca gaaaaaattg
     8401 ctgcatttac agcagcatta aataaaacat ttggacaagg agatgtaaaa taatgacagt
     8461 taaaaattta aaaggtgtaa cagctgcaag cgaccaattg atgaaagctt ttaaagatgg
     8521 taacgaagaa tcttttagcg cagctatggt aagcttatct aaggaaattc aggataaaat
     8581 tttagaagaa gcaacagcaa aaaatcaaga tcaattagta ttaatgaacc gtggtcagcg
     8641 tgtattaact acacaagaaa caaaattcta taacgaagta gtgaaaaacg aaggttttgc
     8701 aggggtcgaa gaattagtgc cagctactgt atttgaacgt gtatttgaag atttagaaca
     8761 atctcatcca ctattgcaaa aaattacttt tgttaacaca actggtgtaa cagaatggat
     8821 tgtgtcacgt ggagtcaatc cagcatggtg gggtaaactt tgcgaagctg ttaaaaaagt
     8881 tttagataat ggctttgatg taattaacat gaagcagttc aagctatcag gttatattcc
     8941 tgtatgtaag gcaatgcttg atttaggtcc agtatggtta gatcgttatg tccgtactgt
     9001 tttagtagaa tcattgagaa ttgcattaga acaagcaatt gttgatggta ctggtaaaga
     9061 tatgccagtc ggaatgatgc gtgacatgag caaacaaact agcggagaat atgctgaaaa
     9121 aaaagcagaa cctattacag ctttagatgc tgtaactatg ggcggtttga tggcgcgact
     9181 atcaaaattc aatatcgaag gtgtgaatga tccgatttat cgtaatgtga atccttctga
     9241 tgtggtccta attgtgaatc caactgatta ctggtctaaa gtattcccag ctaagactgt
     9301 actaactgct aatggagaat acgtacaagt attgccagta ccagtttcag atttgcagtc
     9361 aacggctgtg ccagaaggaa aagcagttat tggggtagcc tcagattact tcatgggtgt
     9421 aggatctaca ctaaaaattg aagcttcaga tgaataccat tttgttgaag acgaacgcat
     9481 ttatctagct aaacaatatg caaacggtca acctaaacgt aacgatagtt tcattgtatt
     9541 agatattagc gctttgggaa ctactactac aactacaaaa ccaacaacca caacaactac
     9601 aacacaagcg taggtgatca gaatgaagta tattctttgt cagccggcaa tcaatcggtt
     9661 taaatgggag cttgaagttt gtttaactaa tctgaagaaa ctaggaatca aagatatcgt
     9721 attgcttttc agcagacacg atgatcagat tcctattttt tttgagaagg aatatggtgt
     9781 tgaagttcat gtgtacgatg atctgcggga cgacaaagag tatattcctt cgattaaacc
     9841 atatttatgg tggaaatatt tagaagaaga tcattcgcgt gaggacgacc gatatttcta
     9901 tatcgattcg gatgtcattt tcaataaaag aattaatttg cgcaaattgc cttctaaaga
     9961 tgatgtttgg tattgtagcg actgctgtag ttatctaagt cttgattata ttagaagctg
    10021 tgaaaacgga gaaaatattc taaaagatat ggcaaacatt gtaaatgtta cagtagaatc
    10081 tttggaaact ataaacacta attcaggagg cgcacagtgg gttattaacc gtcctaaagc
    10141 gaattattgg aaaaaggttt atctggattc taatcggcta tatcgctacc ttagagggca
    10201 aaaaacaaat atacaaatct ggacagccga gatgtgggca cagctttgga acatgatgta
    10261 tttcaatatt ggtcctaaag ttcacgagga attagacttt tgttttgcta ctgatccaat
    10321 agaaaaagtt aaagaagtaa aaatcttaca caatgctgga gtaacaacaa acgatgaaga
    10381 tttatttttc aaagggagat acgtgacttc tacgcctttt gatgaagatt tatcatttgt
    10441 aaacaagaaa aaatgctctt acgcatatgt taaagcaatt aaggcggtgg ttagatgacg
    10501 cctgaacaag tgactgaaga attgctaata gctgtgaagg ataatattta cgttacctgg
    10561 aacgaagaag atgagtcaat taaaaagatg atagctaaaa atgctgttta tcttcaaagt
    10621 aaagtgagta caacactttc tttttctcct gaaagcttag aatacggatt gctaatcgaa
    10681 agatgtagat acgactggaa tcgtgcttta gatgagtttg aacaaaattt cgctagtgag
    10741 ttattaggtt tcattcaaca ttatgcgcta caagaatata ttgcaggtga tgtgaatggc
    10801 gaataatcgt agactcgaag aaacattcaa cgatggttgg ttaaagattt tgacgcaaac
    10861 taccaaaaga aatgaactag gaaaaaagat tggtgtagaa gatacagaaa tcacttcttt
    10921 aaaatttaga aatctttcca tgagagatag tgatataaca gctatggatg cgatgggatc
    10981 gaaattaact aagaaagtaa agactccatt tcatccaatc gccaagaaat ttaataaaga
    11041 tcaatatttt atcgtaatcg atagtatgcg ttacaacgtt atctatgccg attacgataa
    11101 tttttatatc tatttttatc ttgaaagtgt gggtgaatat ggtgattgat aattctaaag
    11161 aaaaagaacg tttaaataag caaatttctg ctatcaaaac ttccttagaa gagcattttg
    11221 gcctcaaact ctttcaagac tccgtaggcg aggatgagct acctgatgat tttaattact
    11281 tcattctcga aacaggagaa atagaaatga tcactgagcc aaaatatagc gtgggtcaaa
    11341 atctatatct aactttctat tcagaaaata gagaagattt aacaggagac tcactagata
    11401 ttatttcatt gattcaaaat cgttcgattc gttttcagag aatggatccc aatcatttaa
    11461 aactagagaa ccaagatcgc tatatcgatc aattggtatt tacgtttaga cgattattga
    11521 agagtgattg tcatggctaa aaatagttgg gagctaaaaa taaatggaca tgatgaactt
    11581 cttgtgcgga tggaacgcta ttcaagcgag agcgaacgac tgattaacga agcattgaaa
    11641 tcaaaaggtt cggctattgc agtggatagg attacagaaa aaattcctgt ttctgaagca
    11701 gatttaagaa gagggcacca acacgcaaaa aatagtcgtc cacttaagac tcaatacatt
    11761 aatttgggtt tcatcattag acctacaaga aaatttgagt atttaaaata tcctgatttg
    11821 gggataggta cttctaaaag aaatcagccg gacgaattta tgagaagagg attaggtctt
    11881 gcacttgatc caattacaga acttctgatt cgtcaattcg ataaattaaa taaatagggg
    11941 gaacaacaat ggctaaaaca aaaactgtag taacaacgtt cgataacgta agtatcaaac
    12001 gaattgcttt taattttaag aacgcaggaa atgcaatcgc aacgaattgt aatggacaat
    12061 tagatggcga aacagaaatg caaacggtgg ttaaaaaatg tggagcgaca gaagtaaaat
    12121 caaaatctaa accaatcaat atgacggtaa caattactgc acatgtaccg atggaagttt
    12181 atcgacgttt caatgggttg aaacaagatg gacgtattaa accaggcatt tactcttacg
    12241 gtcctgattc cgtaggcgaa gatttctcac ttgctgcaga gatcgtggat gacttcgaag
    12301 aaaatagaaa gttagttggt atgttagcat gcacttcgaa tacaggatta acattctcta
    12361 ttgaaaatgg tgcggatgaa gtagctgcgt tagaactaga aacaaaagtt atgcaagatg
    12421 aatttggtaa attttatcat gaagcaattg ttgcagaact tgaagaagac ttaacagatc
    12481 aatggatgac gaatctatct gctgatgtga ttaaaaaaac ttcagttgtg acaactacgg
    12541 ccactcaatc acagtaaaaa aaacggaggt agcgaaatga acgaagatta ctcaaaaatt
    12601 gaactaaacg atggaacaat tttgaattta gaacctaaac tgaatatcaa gaaattattg
    12661 atgatcaata gagattttaa cacagacgag tttgcaaaaa tgactgtggg aaaaggatcc
    12721 atggatattt ctgttattca aggtgcaaag gctgtgtata ttgcttaccg ccaagcgaac
    12781 atgactgatt atatttcatt cgatgaattt atcgataaat gggattttga tatggctact
    12841 gccagctata tttatcaatt gatgatgttc aaacaagcac gcgatgctta tcaaaaagaa
    12901 tttgaaaaag caaataagga aaaaaagctt caaaagtaaa aattccaaag ctcttagttg
    12961 aaacgtgggt cgatgtctat tcgatgttga ccgacgtttt ttctatgcct tcagatttgg
    13021 ttttaagcga tatctgttta gatgacattt tgcaaatggc ttacaacaag agtgcttatg
    13081 aaggatggaa aaactatgca ataaaccaat cccagaaaaa ctaaagaaag gaggtaaaaa
    13141 atggctaaaa agagaacaga agcagaagta actttcatag ctaacgatga cggattgaaa
    13201 tctacgttaa aagaaatcag tgctgaatta actaaaaata gagcagaatt aaaactagaa
    13261 caagctcaat tacaacagac tggttctgaa tcagacaagt taggaagtaa attatcttct
    13321 ttagaaaagc agtatgaatt acaaagtcaa aaagttgaag taactagcca acgtttagcc
    13381 aatgcgaaaa aatattatgg agaaaattcc accgaagttc agaaacttga aagagaactg
    13441 attaatcaac aaacagcgca acaacgtttg tcaaacgaaa ttgataaaac gagtaatgca
    13501 ctagctcaag caaaaggcga aatacagacg tacgagtcta caatgcaaca gttggatagc
    13561 gaacaaaaaa atgttcaagc tagtgcttct ctgattgaat ccgaatacaa aaaatggcaa
    13621 gcaactgctg gtcaatcagc ttctgaagcc gagaaattag cgaaagccca agaatatgtt
    13681 tctcaacaat ctgaaaatgc ggagaaaacg atagatatcc taagacgaca gttagaagct
    13741 acacagtctg aatttggcgc tacatccaca gaagcaatgc agatggaagc gaagcttaat
    13801 gatgctgaac gtgaatttga agagttagga caagctgcta aaaatgtaga tacaactaac
    13861 ttggacgata tcggaagcaa aatagatatg aataatttaa tggaagcttc tgacgtttta
    13921 agcgacattg gcgataagct tacagaatta gggaaacaag cagtggactc tgctaacagt
    13981 gtaggtagtt cccagagtaa gatacaagct aattttggtt tgtctaaaca agaggctgaa
    14041 gaattaacga atgtagccag agacatttat tataaaggtt ttggagaatc gttagatcag
    14101 tccacagatg cattgatttt ggtaaagcgt aatttaggag atttaaataa tcaagattta
    14161 caaaatatca cggaacaagc tatggtccta gaaaacacca tgggcgctga tatggatgaa
    14221 acgttacgtg gtgtaaatgg cttaatggtc aatttcggct tgagtgctca agatgcaatg
    14281 gatttaatgg tttcgggtac tcaaaacggt ttagataaaa cgcacgaatt aggcgacaat
    14341 atggcagaat atagccaatt atggagtcaa atgggatatt cagctgatga aacgttcgga
    14401 atgcttcaaa atggtttaga tgcgggtgct tataaccttg ataaagtcaa tgacttagtt
    14461 aaggaaatgg gaatatcgtt aacagatggt cgatttgagc aaaacatgga tatgtttagt
    14521 gaaagtacta gaaaagcttt tgaagagtgg aaaaatggcg gaggaacaca aaaagacgtt
    14581 attaattcca tgattcaaga ttttagcaat atggatggtc aatacgacca attaaataaa
    14641 gcttcgacaa tttggtctgc gcttggcgaa gataatgcga tgaaagttgt ccaatcttta
    14701 actgatgtta accatacatt tgatgatgtt agtggatctg cacaaaaaat gaatgaagat
    14761 tctactactc cgttgcaaga gttgaacggg aaaatagctg aattaaagga ttcattagct
    14821 cctatagaca acacaatcat agatgcactc gaaccagtaa ttgattttct aggaaagatg
    14881 gctgatgcgt ttaataatct tccacaacca gtacaggatt tcattgttgc tataggtggt
    14941 cttacagcag catttggcat actgttaccg gcaatactag ccgtgtcata tttatttggt
    15001 ccgatgatgc ttataattgg aggaattata gctgttatag caggcgttat tgtggcaatt
    15061 aagaactggg gtgcaattac tgactggttt agtggtttat ggaaaaaatt tactgattgg
    15121 ttgggtgata cttgggaaag tataaaagac ggagcctcat cagtttggga tggggttaaa
    15181 gaaacctggt ctggatttgt agattgggtt caagatattt ggcaaggagt ttctgattgg
    15241 tttggagagc tatggagcgg attagttgaa ggagcttcca acatctggca aggagtccaa
    15301 gaaacttggc aaacattcgt tgattgggtt tcaaatattt ggaacggagt caaagaagta
    15361 tggtcgatta tttgggcaga cattgtagga attgttcaaa taccatggac attaataacg
    15421 tcattgattc aagctggtat taatattatc gtgggtattt ttgatgtagc tggacagtta
    15481 ttgggcgcag cttggcaagc tgtttggaca cctatttctg atttccttaa aaatacttgg
    15541 gatactatga cacaatggat aagtatcgct tggaatggga ttgtaactac attccatact
    15601 atatttgatc cagtagtggc atggtggaat ggtatatgga cagctattag tactacggct
    15661 tcaaatattt ggaattcaat tagtgcaaca gcttctagta tttggaacag tatcaagaat
    15721 acaatcacta gcttggtaca agcagctgct acagtaattc aaaatatttg gtcaactgta
    15781 tctagttggt taggtggaat ttggaattca atcagctcta cagcatcaaa tatctggaat
    15841 agtgtgacta gtagtataag caatgctata aacgcagcta aaagtgccat tcaaagtgtt
    15901 tggaatagta tatcttcgtg gattagcgga atttggaacg gtatcaaaaa tactgctttg
    15961 aatctttgga atggaattac aagcactatt agctctaaag taaacgatgg aaaaaatgca
    16021 atttcaagcg gttggtccaa tctaacaggt attgtttccg acatattcaa taatgttaaa
    16081 agtacaattg ctaacatatg ggaaggcatc aaaaagactg ttagcgctcc aattgattgg
    16141 attagagaca aaatcagtgg catttttgat aatttgaata tttctatacc acatattccg
    16201 ttaccggaat ttatcatgga aggcagcttt aacccgctaa aaggtcaaat cccccatttg
    16261 cgtgtaaaat ggcatgctaa aggaggtatc tttactaaac caactttact aggtggaatg
    16321 aacggtgtcg gtgaagcagg acctgaagca gttttacctt tgaaaagatc tgttttgcaa
    16381 gaaattggtg atcgtatctt gagtagcaca tcagtttcat ctagggcaca aacgattcaa
    16441 cctgtgaata actacgaatt caatttcaca attgatggta acgcagatga ggttactatg
    16501 aagcaaacaa ctcaacaaat cattgatagc attacaaaaa ttcaaaatga taatgcttcg
    16561 gcatggcgtt aaacaggaga gtatttctcc tgttttttta gtattaaaaa ggatgtgaaa
    16621 aaatgactga ttgtatacat tctataatcg atggatttcc tgattatttg cataaattgg
    16681 ctttagctga aagaccaacc ataccttctc caaaaagaca gagagttgaa acttctgttt
    16741 taggtaggtt aggtggctta gtacaagatt actcgtttga agatatgtcg tttacattgc
    16801 actataacta tttagaggat gtggaagacc atcaagcgtt caagcaatcg ttttatatca
    16861 tgcgtcattg gttaaactat gcaaagaaat tagaattctc tgacgatccc aacgtctatt
    16921 acgttatcca gactatcgat attggggatg cagaaaacga tattgttgaa tggggagagt
    16981 tcgatgtaaa tattactgcg aaaccattcg caagagttca agaagatgta cctataaccg
    17041 tagataaacc acagtcattt aacttgctga ataatagttt agaagaaagt tttccaaaga
    17101 ttatcatcac tccttcagct acttcatgcc agttcatctt aaatgattat gtgtttagtt
    17161 tcgaaggctt agtagcagga actgatgtag tcattgatag tgatttgatg ctttgctacg
    17221 aagagcaatc ggacggagat attttagatc ggtccaacaa aatgaagacc atgcaatatc
    17281 cgacattgca agtggatatc aatcatttta attgcactgg tttgagcaaa atacaaattt
    17341 atcgtaacgg gttaaggtag gtgaaataga tgatcgataa tttaataact atttacgata
    17401 aaaatgacgc gaataattta gctgaacatt tatatgatac gcaaggttta ggcgctttgt
    17461 cagactggtt aacagctact gttagcaata aactaaacgg agccgagata tttcagggta
    17521 cttattcaat aaacggaact aatgcagatt tgattgtaga cggacgtatt attcagtgtt
    17581 atgtagatga aaatcgagca aaacagcgtc tacggattta ttatgcaaaa acttctgtaa
    17641 taggcaatac gatagaagta aaagctgaac ctattttcaa tgatataaga aaatcggtgt
    17701 tgaataaata tgacagcgga acagaaaaga tcactgctac tcaggcatgg caaaacgcaa
    17761 aagttttagc gaaaccagct atcccttcgc agttttcttt ctcgtcatta gtagatacgc
    17821 ttgctaatgt gaagatagaa aaggcgaatt ttttagaatt ctttggtgga aaagagggat
    17881 ctattctaga tcgatttcat ggtgagtttc taaaagataa taacacatta cgtcatgaaa
    17941 aaagtctagg tacggatcat aaaatcaaag cgatttatac taaaaactta actggtcttg
    18001 acttagagat tgatgctcaa agtgttttag ttggagttta tccattcatt agcagctctt
    18061 cagaaggaga agacgagatc actctaccag aagaagttat tttcacggat tacgtggatg
    18121 attatcctgc tggatatgtt tcttttgttg attttaaaga caaagcgact gatgtagcca
    18181 cattaaggga agctgctaaa gactggttga aaacaaacat agataaacaa aaaccacaag
    18241 tgagtggttc gattgaatta gtaccattga ggcatcaaag aggctatgaa aaatttgttg
    18301 atctagaaaa agtttcgatg ggtgacggag tagatgtgta tcatccacag ttaaaagtga
    18361 atatgtcagc gagaattgtg gaatatacgt ttaatgttct aaccaattca tacgataaat
    18421 tagttgtagg aaacgtcaaa acaaacttct tagaaaacac agagaataat gtcagcaatt
    18481 tgattaatga tgccattgat caattgaaaa acggtggcga aatcagtgat ttactcaatg
    18541 atattgtaga tcatcaaact gatatgatta ctggtcaaaa tggtggttat gttttattag
    18601 atcctaaaga agcgcctagt cgtattttga ttatggacac accagataag aataccgcaa
    18661 ggaatgtttt acaaatcaac aatgctggta ttggtttttc taaaactggc attaatggaa
    18721 catatgaaac ggcatggacg ttagatggcg gattcaatgc ctcgtttatt acggctggtg
    18781 aaatagtagg aattactatt agaggtacta cattaattag tgatggtgct gattatagaa
    18841 caagtattgc taatggcaaa atgacttggt actcaaaaaa agttaacaaa gatattatgg
    18901 agctagaagc acgtgattat gtaagtgctg atgccggtat tgtatcatac accatgaaaa
    18961 ctggtggtgg tttcatgatt agaaatccac agggtaactt ggtttttagt acgtgggata
    19021 atggtaataa cagaccgttt ctatcttttg gtgcgcccaa tttcaggtat agcaatgcta
    19081 gttatgtaac ttctggcgac ggtagttctt taagcattaa tggtagtgcg ggtaactcat
    19141 gggaatttaa ggtagctggt aggactatga aatttactag tgatggtatg ctaacgttac
    19201 caggttgttt ttttggttca tgggaagatg ggaaacttgc taggtttgaa caatcaacgg
    19261 tacaagtata taaagatttt actgttagag gtactaaaaa ctcaactgta ccaacagaac
    19321 attatggaca acgactattg aacgcttatg aaactccaga atattatttc gctgattatg
    19381 gggaagccgt tacaggtgac aatggtaaag ttcgtgttga tattgacccc atgtttgctg
    19441 aaacagtaaa tctaagtcgg tatatgacac atgtgacacc tacagaacta gttttgtgtg
    19501 ctgttactca tgaagatatt gaccatttca tcattgaaac tagtaagcca aacgtattag
    19561 ttagatggaa tttagtggca caccgtctag ggtatgaaga tattagatta aaagaggata
    19621 cagcatatga tagcacagtg cttgaccaaa aacgttttta aaacgaagac aaggaggtat
    19681 ataaatggct agcagtttat ataatttggc tttagatttc agcaaagaat taaactacac
    19741 caaagctatt atggctcgtc aaggtgataa agggattacg gtgacggtta aaccgtttct
    19801 aaatggcttg cagatggata cgagtggcgg aacatttact ttaaaaggaa caacaccatc
    19861 taaccgttac gtagataatg ttgcaactag tgtaactagt gaagaagtca cgttttctct
    19921 tgatggcaca tttatgagtg aagcaggata ttataaacac tgctacgtag aatatagaaa
    19981 agacaatcaa attttaacaa cgcaagatat catttttttc tcactaggag tgtctgacat
    20041 ttcgcaaggc caagccgatg aatatgtttc gcaattagaa gagttgattc gaaagtacaa
    20101 cgaaactttt gatgctttta tggctgaaat caaaggtaga gtggatagct taaatcaaca
    20161 gattactgat ttaactggtc aagctaaaac gctacaagac aagttagatg ctctgaaaga
    20221 agaaatttct aagttaggta acttacaagt gatgtacagt aacagcatcg acttcgggga
    20281 ctatgattat tcggagaacc cgaatttaat gccatatatt acagaacctt gggttggacc
    20341 tttgttaggt aatggacata cagttaaaga tagtgttaaa agggttataa ctcatactaa
    20401 aactagaaca gctaattccg gcgacatatt atcgcttggc ttaggtattc cgtgtacagc
    20461 ggaagctaat aataggtatc ttataaccac tttacgacca tctacgacat acacattaag
    20521 cgttactatg agtgtcggtt cagattggac tggagagacc aatactatag gggttaggct
    20581 tagatattta aatgaacaag gtggtataga actgcccatt aatgcattaa taccagcaaa
    20641 tgtagaacgc gataaaatgg tcactcatac atttactggt ataacaaaag ataatgtaac
    20701 tagcataaca aactgttatg ttgaaatatt ctcacttaat agtgaatata aaggaacggt
    20761 tagtgtcagc tatgacgtta agttaaaagc acactaccct aacttactag atggccctta
    20821 ctggttaggt aaagttcctt taggcgagaa tattgctgac cctacagttg tctttccaca
    20881 caaaactagt gaatatatgg tttatggtag acgcaataca gaaaactata tcgctgacca
    20941 aacatatacc atatcaatga aagcaactaa actaacagtt caatcgtttg ccgtgtatat
    21001 tgctgctggc cgcgttaaag taggagacat gaagcctact gaggggttag ctaatacatg
    21061 ggagttaact tttactgtaa ctaagcaaca tatagatagt ggggtaacta attatctgga
    21121 aatataccaa tacccgtcag ctacaaaagg agctgttcaa attgagtggc tcaaactaga
    21181 aaaaggcaat actcgaaccc cgaatattag tgagtataaa tatcgtggta ctggtatgcg
    21241 tgattcaaac aatccaaaag attatgtttg ggatctagca ccagaatatg tcgaagataa
    21301 tttggccaca gatattaaaa tttctgaaat tactggtaaa gcaaacaatt ataccgatgg
    21361 gaaagtatcg gagattaatt cgcagttgac tgcttcaatt aatgaagtag acaccacagc
    21421 taaggatgct caaacaaaag cgaatgctaa tgcgactgct atagatgaat tagacaataa
    21481 gatcgatgaa cgcattaatg atacagctac taccacatta acagttacaa acgggaatac
    21541 cggatcagca aagctttatc gtgaaggaaa aacagtttct atatattttg tggctttaaa
    21601 cggaaaaagc agtggtggaa atgattcaac gatactaaca attccagaag gctatcggcc
    21661 accaattagt tttgagcaac tggttggctc gatagaccgt tctactttga acagtgctca
    21721 gttatctatt ggtgcagatg gagccattaa atggcgaaga aactcaagtt atggatcgga
    21781 ttataccttt gcaattactt acacgattta gaaaagcgtg aatcgatatg aaggcagcat
    21841 atagaccaat tgaaccttac ggattcgagc aaatcattgt gaatgatgaa gaacatttac
    21901 cggaagaatg cacagaagtc gaaccaccga ttccaaattg gaaaccgaaa ttcaattact
    21961 gggagggaaa taaatgaaaa acatttggaa atatggacgt actggcggag agtacgcagg
    22021 aaaagtattg gacgacatgc ttgtatccgt tccttacaca gatcagccac cgcttgaagg
    22081 aattcgtgct gatggcgaac cgttaacaat cgctgatcaa atgtttgatc ctaaattgaa
    22141 tcaatggatt attttagcga acgcactaga tcacaacgat ttaaacaatc tcaaagcaat
    22201 gtatgagtcg ttagaaaatg agaacggcga tttaaaacag atcaatgcca aactcatgct
    22261 aagcgatgta gcaattaaac aggaaaatac tgcattgaaa gaaaaagcgg atagtttagc
    22321 acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat agcaaagaca tttcagaaat
    22381 taaagagcaa ctaaatccag cttcaaaggg aggtgagtag tatgtttagt tttagcgatg
    22441 tgaaaatgat gtatgattgg ggctgtttca ctaacgaaca agtaatggtt ttcgttccgt
    22501 tgtgcattac tgaagaaaaa gcagataaaa tcattagcaa agaagagagc gcatcttaat
    22561 tgatgtgctt tttattttga ttcaaggagt tgtcacatga ttaatttagg ggaatgggga
    22621 gcgatagcag gatcaataac cgctatcgtt tctttgattt tattagtaat aaaaccaatt
    22681 actgcatctt tctcgaagat tactgagact ctttcaaaag taagtcacaa tttagatttg
    22741 ctgactaaag atttagaatc gagcaaatca gatcgattga tgattcatga agaactaaag
    22801 aaacacgatg aaagattaga tacacatgca gaaaaattgg tagaacacac gcaacaaatt
    22861 aaaactttat ttagggagag aagaaaatga ataataaaac gttcgaagta ctaaaatggt
    22921 tcgcactggt aattattccc gcactagcta ctttcgtggg gttagttggt aaagcgctca
    22981 attggcagta cacagatatc tgtgttgtca tcattactgg ttttggcgcg tttttaggga
    23041 gtgtgttggg tgtatcaaat cgaacctaca aaatgttctc ggctgaaagc gaagaaggag
    23101 gaaacaaatg aaaaagaaaa ttactattac tgcgatgagc ctgttaacgg ctcttttttt
    23161 attacccatt aatacgtttg cttatactat taatgacgag tataatttag cgccgaatca
    23221 aggagactcc agattagcaa ttcctaacaa aattattttg cacgaaactg gaatagatgc
    23281 accagcaaga aacgtagccg ccaacatgaa aaataattat aacggaagca attcttatac
    23341 tacagatgtt attggtgacg gtgggattgt ttaccgtgtg ggtgagcaag gatatgtttc
    23401 gtggggagct ggtaacgcta atccttatgc gcctgtacag attgaattac agcgcacata
    23461 tgataaagca ttgtttgaaa aaaactatcg agcttatatt gaatatacaa gagatagtgc
    23521 aaaaaaatat ggaattccat tgactcttga tcaaggaact tctttattta caaaaggaat
    23581 catttctcat ttgtgggtga caaattatgt ttgggggaac cacacagatc catatggtta
    23641 cttatcgcaa atgggagtta gcaaagaaaa gcttgc
//
LOCUS       DACDIP010000041        23077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDIP010000041 DACDIP010000000
VERSION     DACDIP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.041"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(186..311)
                     /locus_tag="IV257_001793"
     CDS             complement(186..311)
                     /locus_tag="IV257_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8814898.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(445..1338)
                     /locus_tag="IV257_001794"
     CDS             complement(445..1338)
                     /locus_tag="IV257_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8814899.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1520..2446)
                     /locus_tag="IV257_001795"
     CDS             complement(1520..2446)
                     /locus_tag="IV257_001795"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8814900.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2531..3292)
                     /gene="pflA"
                     /locus_tag="IV257_001796"
     CDS             complement(2531..3292)
                     /gene="pflA"
                     /locus_tag="IV257_001796"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8814901.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3536..5767)
                     /gene="pflB"
                     /locus_tag="IV257_001797"
     CDS             complement(3536..5767)
                     /gene="pflB"
                     /locus_tag="IV257_001797"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8814902.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6044..8494)
                     /gene="parC"
                     /locus_tag="IV257_001798"
     CDS             complement(6044..8494)
                     /gene="parC"
                     /locus_tag="IV257_001798"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8814903.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8506..10551)
                     /gene="parE"
                     /locus_tag="IV257_001799"
     CDS             complement(8506..10551)
                     /gene="parE"
                     /locus_tag="IV257_001799"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8814904.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10717..11151)
                     /locus_tag="IV257_001800"
     CDS             complement(10717..11151)
                     /locus_tag="IV257_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8814905.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11278..11916
                     /gene="plsY"
                     /locus_tag="IV257_001801"
     CDS             11278..11916
                     /gene="plsY"
                     /locus_tag="IV257_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8814906.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12048..12923)
                     /locus_tag="IV257_001802"
     CDS             complement(12048..12923)
                     /locus_tag="IV257_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8814907.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13005..13796)
                     /gene="codY"
                     /locus_tag="IV257_001803"
     CDS             complement(13005..13796)
                     /gene="codY"
                     /locus_tag="IV257_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8814908.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(13814..15214)
                     /gene="hslU"
                     /locus_tag="IV257_001804"
     CDS             complement(13814..15214)
                     /gene="hslU"
                     /locus_tag="IV257_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8814909.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15228..15776)
                     /gene="hslV"
                     /locus_tag="IV257_001805"
     CDS             complement(15228..15776)
                     /gene="hslV"
                     /locus_tag="IV257_001805"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8814910.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(15813..16718)
                     /gene="xerC"
                     /locus_tag="IV257_001806"
     CDS             complement(15813..16718)
                     /gene="xerC"
                     /locus_tag="IV257_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8814911.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(16916..18292)
                     /gene="trmFO"
                     /locus_tag="IV257_001807"
     CDS             complement(16916..18292)
                     /gene="trmFO"
                     /locus_tag="IV257_001807"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8814912.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18261..20339)
                     /gene="topA"
                     /locus_tag="IV257_001808"
     CDS             complement(18261..20339)
                     /gene="topA"
                     /locus_tag="IV257_001808"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8814913.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20461..21318)
                     /gene="dprA"
                     /locus_tag="IV257_001809"
     CDS             complement(20461..21318)
                     /gene="dprA"
                     /locus_tag="IV257_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8814914.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21374..22141)
                     /locus_tag="IV257_001810"
     CDS             complement(21374..22141)
                     /locus_tag="IV257_001810"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8814915.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22134..22994)
                     /gene="ylqF"
                     /locus_tag="IV257_001811"
     CDS             complement(22134..22994)
                     /gene="ylqF"
                     /locus_tag="IV257_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8814916.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6028 a         4997 c         3982 g         8070 t
ORIGIN      
        1 atttatgtgg caaagaaaaa acaaacaaaa taaaaagcat tttgaagcct gactattacg
       61 acttcataaa tgctttttta gttttaaaca cttcaatcta aaagaataac ccaagattgg
      121 ataaattatt aatgactcgc cattcgtttc ttagtgattc tggattatca aatggacttc
      181 ctaatctaat cagctagttc ttcccataaa atgccatact tgactgaatc ataatattga
      241 ttatttaaaa atctaacttt tcttatcact acttcctttg tcattccaca tttctcacca
      301 tttttttgca ttcctttgtt tccagaccat gtagtgaatc taataggtgg tagataggca
      361 aaatactcaa ataattcttt taaccataaa ggaagagaaa ggctcctcct gcaagagtca
      421 gtataatttt tgcttactgc tctcttaggt tttactcact aaaactgttc catattcttt
      481 gatataagaa aatgcatgcg gtataaattt cttgctcagc tcagaaaaga tttgtcgttt
      541 aagaaaggcc atttcctcca ttgtcaatga aggatccaat gttggtaatt caatctcaaa
      601 attgtttttt tcgacaatag gtaattctaa tgtgaatgat tcttcttcct ttatagtatt
      661 cgtcacaagt agagactgat ggaaaatact aggccggcga tttttatatt tattcttacg
      721 tatcctgtca acgataactt ctatttgatc aaaaaagtac gattcattta cgtctccaat
      781 aagttcggct aattctttga atgccttcgt tacaggaagc tgattcttct gtacaaaaaa
      841 gtaattagcc aaagtaagcg ggaagtgttc tatagaggaa agtgtgataa aacggtactt
      901 ctcatttcct gcagttgtcc tcagcaattc tgtctctcgg ctgtttctta ctccataaaa
      961 atacgcttct ttccgttcat cttcgaaaca atacgcccaa ataacttctc ccatcgctaa
     1021 tttttgttca tcaacagaga gtctcttcaa ttgttcagct atggtttcag cagccgaagt
     1081 ggcttgttgc aagtaatctt taggatcaaa tattggaaag ttcaaatgat atcttcgttc
     1141 ttccctcttg attaatcctg catcgatcaa taaatcaaga aaatgggaaa atttattttg
     1201 cgaaaattct tttttcaatt ctcttagaat cgtttcttgt tctcgatgtt catatagata
     1261 atcggtcaat gaagaaaaaa gcggatcatc ggtcagtttt tgataaaagt tttccttatt
     1321 ttttgaataa tagttcatat atgttctctt ttctatccgt tttattttac ttaaaaggct
     1381 tttacgtaaa aaggaggaag aacaagagtc ataattgact gctattctcc tccatttctt
     1441 cggcctttag tatgttctat gtttcttttt atatcaatcg cttatccatt cattttgaat
     1501 gcttcatact accatttgat tagctaaata cagccgtcaa ttgaggtacg acttgttttt
     1561 tacgtgaaac gacaccttta agcaaagcac ggttgttggt caaagttgtt tggaaagctt
     1621 cttctacttt ttcttttcct ttaccagcaa caagtaattc tgaatcactg tctaagatgt
     1681 ttgtgacaac taatacgaac agatcgtagc catttgcttt catttcatct tccattgctg
     1741 cttctagctc tgcttgtcga tccatcactt cattaaggtc cactgtattg acttgagcga
     1801 tacgaacgtt ggaactgccc attgggaaac tttttgcatc caaatcaaga agaaccgcag
     1861 ctgatttatc acttagattt gttcctgctt tcaacatttc aagtccataa gtgtttagat
     1921 cgacttcagc gattgctgca agttcttttg cagctgcttc atcttcaggc gtacatgttg
     1981 gtgatttgaa caacaatgta tctgaaatga tagctgagag catgattcct gcgatttctt
     2041 taggaattgt tacttggttt tctttatata gttttaaaat aatcgtactt gtacatccaa
     2101 ctggttctgc acgatagtac aatggattag ctgtttcaaa gtttgcaata cgatggtggt
     2161 ctactacagc taaaatattt acttcgctga tatctgaaat actttgttgg aattcattat
     2221 gatccacaag catgacatct gcattttctg ctttagctga ctctactact cgtggtgcag
     2281 atagctgaaa gtggtctaat gcgtattgtg tttcttcatt aggtgtacct agagcaactg
     2341 cttctgtttc tttacctaat tcattttgca agtgtgaaaa cgcaatggct gccccgattg
     2401 catctgtatc tggattttgg tgaccaaaaa ctaaaatttt tgacatgtca ttcgctcctt
     2461 tgtgatttct cctatttaat aatagcaaaa aaacggaaag aagcaatact tatcttcttt
     2521 ccgatgcgac ctatcgcgtt aaatattttg tatactcttc tgtatgaagc aatcgtttag
     2581 cattttcgac acgttctttt gtaggtggtt cgatcccttc taatggatat ttcaaaccaa
     2641 gtgtttccca cttgtatttt cccatcgtat gataaggaag aatctctact ctgtctacat
     2701 ttgacaaggt ttgaataaag ttgtctagtc ggatcaaata ttcatcataa tcacttcgct
     2761 caggaactag tacatggcga atccaaaccg gtttgttgat ttttgacaaa tattgagcca
     2821 tttctaaaat attttcattg tcccaatgcg ttagtttttt atgctcttca ttatcgatgt
     2881 gtttgatgtc aaataacagt aaatctgtat acttcatcaa ttcttgaaaa cgtgaaaaga
     2941 aaggttcttc atacgtaaaa ggttttccgc atgtatccaa tgtagtatgc atcttccgct
     3001 cttttgcttt tttaaatagt tcgatcaaaa aatcgatctg caatagaggt tctccaccac
     3061 taacagtaat accaccttta tctccccagt aaggtctgaa gcgttctgct ttatctaaaa
     3121 gttcatctgc tgtgtattct ttccctccgc caatattcca agtatctgga ttgtgacaaa
     3181 attggcaacg catgcggcat ccttgcataa acacgacaaa gcgcagtcca ggtccgtcta
     3241 ctgaaccgaa agtttcgata gaatgaacat agccgattgt tttttcttcc atttaagttc
     3301 acatcttttc tttgatttca ttttaagtac aagttagctg tcgaagcgaa gctttctgaa
     3361 caaaattttt cgcaattgcc gcttcgttat gtgtaaaatt tacagggacg gaacatacaa
     3421 gtcctgcccc tgtaaatcgt ttctttcaaa taagcatact gattattcga ttttgatgcg
     3481 atggctattc aacgtccttt cgacgctaag tcgaaaaact tttttcacga actttttaca
     3541 ttctgtcgtg tgaagttctc atcaagacat ctaattgttg ttctcttgtc aagtcacgga
     3601 atttaactgc ataaccagat acccggatcg tcaagttcgg atatttcatt ggattttcca
     3661 ttgcatcacg caataaatca ttactgaata cattgacatt caaatgataa gctccttttg
     3721 agaagtatcc atctaagaca ttacgcaagt tttcgattct tgtatcttgt tcttttccaa
     3781 gacctgaagg attgattgtt tgggtatttg aaattccatc caatgcacag ccgtaattca
     3841 ttttagctgt tgagttcagg ctagctaata acccactttg ttccgcattg taacttggat
     3901 ttgctcctgg cgcaagtggt tttccagctt cacggccatc aggagtattt cctgtatttt
     3961 taccgtaaac aacatttgaa gtaatcgtca ataatgaagt tgttggtttt gagttgcggt
     4021 aagttttatg acgtttgatt tgtcccatga agtattccaa gatccatgaa actaattcat
     4081 ctgcacggtc atcatcattt ccgtatttag ggaagtcgcc atccacttca aaatcaactg
     4141 ctattccttt ttcattacgg attgctttta cttttgcata tttgatcgct gacaagctgt
     4201 ctgccgcatg agagatccca gcaatacctg tagcaaatgt gcgttgcaag tcagaatcca
     4261 tcaaagccag ctgagctgct tcataagaat atttatcatg catataatga atgatgttca
     4321 acgtattgac atatagttct gctaaccaat ccatcatatt catgaagtta tgtttcactt
     4381 cttcaaagtc tagtatttct gatgtgattg gacgatattc tggtccaact tgggcgccag
     4441 tcacttcatc gactccgcca ttgatggcat ataatagtgt tttagccaag ttagcacgag
     4501 cgccaaagaa ttgcatgtct tttccgacaa ctgttcctga tacacagcaa gcaatcgcac
     4561 aatcatcaga accccattgt tcacgaagct gattatcatt ttcgaattga atcgaagaac
     4621 tttcgatagc gattctgcta gcatatgttc tgaatccttc tggaaggtgt tcagaataca
     4681 aaacagtcaa gtttggttct ggtgaaggtc ccatatttgt taatgtatga agcagacgga
     4741 aatcattctt tgtaactaat gaacgaccat ctaatcccat acctgcaatg gataaagtag
     4801 cccagatagg gaatcctgag aataattggt tatattcagg tgtacgcgcg aatttgacca
     4861 tacgtaattt cattactaag tggtcgatca attcttgtgc ttctttttct gtgattactc
     4921 cattatccaa atcacgttgg atatagatat caaggaatgc tgagatacgt ccgattgaca
     4981 tcgcagcacc attttgtgat ttgattgctg ctaaatatcc gaagtatagc cattggacag
     5041 cttcttttgc atttgcagct ggtttagaaa tatcatagcc atatttcgca gccatttctt
     5101 tcatttgcgc taatgcacga tattgttcag tgatttcttc acgtaaacga atcacttctt
     5161 cactcattgt atgataacca gtcatatcat gatctttttt cttttgttcc atcaaatagt
     5221 cgattccata caatgccaca cgacgatagt caccgatgat acgtccgcga ccgtaagcgt
     5281 ctggaagccc tgtaatgatt ttgtttttac gagcagcacg catttcagca gtataagcgt
     5341 caaatacacc ttgattatgt gttttgcgcc agtcagtgaa aatatgtgtc aattcatcgt
     5401 ttggtgtata gccattgctt tttagcgcat tgttagccat attgattccg ccgaacggca
     5461 tgaatgcttg tttcaaaggt ttgtctgtct gtagaccgac gattttttcc aaatctttat
     5521 ccaaatagcc tgcatcgtgt gaagtaatag ttgatacgat ttctgtgtcc atatcataga
     5581 caccgttacg ctcgaattgg atatcattta acttttgcaa ttcatcccat aatttttctg
     5641 ttgcttctgt tgggccttcc aaaaagctgt catcaccgcg atattcagtg tagttatttt
     5701 gaataaagtc tctagtgtct acgccttctt tccatttggt acctttaaat ccattccatt
     5761 gctccattgt atggacctcc taaatttctg ttttatgtaa cagtttttac gtgattagta
     5821 taacacagca ccctatcttt gcaagggttt tcttgttttt tatttaacaa tttttatgtt
     5881 ttatttacat atatttaaat caacgttgac ataccaacat ttaagcattg tgaacacttt
     5941 catctttttt tgcaaacgta taaggattta ttaaaaatga atgaaaaaga gacgccacct
     6001 gtattaatac agatggcgtc tcttcattaa aatactacaa cagttacgac tcgatgaaaa
     6061 tctcaggagc atcgataact tcgatgactt ctccaccttt tttctcatcc atcgcaaatg
     6121 atccattcga tgttcgatca cctataggga aatcagttgg gtcaatcgtt aactgctgcc
     6181 ctttttgatt catcaaggtt atttcttttg atcttccttc cgacatatag ataatacggt
     6241 gcggattttt tttcagctca cgcatgaccg tcactccccg cttcgcacgt gaagtttggc
     6301 tgatttcttg agcaagcatg cgtttaacag ctcctcgctg agtaacgatt acgatcggcg
     6361 tatcaccttc tgctaacaca agcagcccgt tgataatcct atctccatct ttcaaattca
     6421 tagacttcac tccagctgcc tttgctccaa caacgggaac atctgttaaa ggatagcgca
     6481 aacccattcc ttgatgcgtt actaagaaaa catctaagcc tggctgatct tctttcagat
     6541 acacagcaat cagttcatct tcttccgcct ttagcttcat acagctgagc ggacggctct
     6601 tataggtgcg ccatggttcg aactctgtca ttcgtgtttg cttgatcaat ccattcttgc
     6661 tgatgaatac aaatgttttc tctgcttgaa ttttttgata tgggaaaaca gctaaaatcg
     6721 attcgtctac agacagattg gagattgttt gagaaatgtg ctcaccagca tctttccatt
     6781 tcaaatcggg tagttcatgg acagggcggt agatcacatt tgctttattt gtgatcagaa
     6841 gaacatggtc caatgtactc aattcacctg agtatagcaa gtagtcgcct tctcgcatcc
     6901 cgatttcttc tggtttggaa gcactataag aacgaaggct tgttcgcttc acatagcctt
     6961 ctcgcgtcac actgacaatt acgtcttctt gtgccactaa gacttgcgta tcaattttga
     7021 tttcttcgat ttcgtcttcg attgtcgtta gacgagcgga agaataattt tttttcactt
     7081 ctcgcaattc ttttttcata acagagaaaa gctctttgtc atttgaaagg attttattca
     7141 actctgtgat ttgcgcaatc aagctttcgg attccttttg aagctctgtg atatccgtgt
     7201 tcgtcaaacg gtataattgc aatgtgacaa ttgcttctgc ttgttcttct gtaaattgaa
     7261 agacgtcgac taaattcttt ttggcgtctt ttttgtcttt actttcacga atcgttgcga
     7321 ttacttcatc cagaatcgat aaagctttca tcaatccttc tacgatatgc tgtctttttt
     7381 gagctttggc taaatcgaat tgactacgtt ttgttatgac ttgctttcga tgctcgatat
     7441 aacttcttaa aatgtctttc agtccaactt gatgcggagt catatggtcg atagcgacca
     7501 tattgaaatt gtaattgatc tgtagctcag tgtttttgaa caaataattc aaaattcctt
     7561 gagcattggc gtcttttttc aattcgacga caatttgcag acctgttcta tcactttcat
     7621 ctcgtacttc agctattcca tcgattttct tattcaaacg gatctcatcc atttttttca
     7681 ccaaggtcgc tttatttact tcatatggga tttctgtgat cacgatttgc tgtttattac
     7741 ccttcattgc ctctatcttt gttttcgaac gaagaataac ttttcctcgt cctgtttcat
     7801 aagctttttt gatttcatct tttccttgca agatccctcc tgttggaaag tcaggtccag
     7861 gaatatattc catcaatttt tcaaggctcg cttgtggatg atcgatcata tagattgtgc
     7921 catcgatcac ttcagctaag ttatgagtcg ggatttctgt tgcatatcca gcagatatac
     7981 ctgtcgatcc attgacgaga aggtttgggt atcgagctgg aaggactgtt ggttcttttt
     8041 ctgtatcatc aaaattccac acgagatcga ctgtttcttt ctcgatatct gctagcatct
     8101 ctccacttaa cttggataat ctggcttcag tataacgcat cgcagcaggt ggatcaccgt
     8161 ccatactccc gttattcccg tgcatttcga tcagtacttc ccgcaacttc cagtcttggc
     8221 tcatgcggac catcgcttca taaatactga tgtcaccatg aggatgataa ttccccatga
     8281 tatttccgac agattttgct gatttacgga atcctttgtc atacgtattg ccatctttgt
     8341 tcatggcaaa taatatccgg cgttgcactg gcttcaatcc atcacggata tcaggcagtg
     8401 cacgttcttg gataatatat ttggaataac gtccgaagcg gtcacccatc acttcttcta
     8461 aagttaattc ttgtacttct tgtcgatttt ccatcaggtg acctcctatt ctacttcaaa
     8521 taactgattg ttttcatttt tatctgctcg ttcttcatct aataaatcat tagaaactga
     8581 aggagagatt tcagtatcct cttttttatc taagatactg ccatcttctt cgagcgtaaa
     8641 ttgcacgtga ttttcgatcc attttcttcg aggttctact ttgtcaccca tcagtgtggt
     8701 tactcgacgt tcagcttgcg cagcatcatc gatacggacg cggatcaacg tacgggaagt
     8761 cggatccatc gttgtttccc acaattgttc cgcattcatc tctccaagtc ctttatatcg
     8821 ctgcagcata tatcctttgc cgactttttt aatcattgct gccaattcgt catctgtcca
     8881 cgcatattca atgacttgct tctttccttg tcctttggat actttatata aaggcggtaa
     8941 cgcgatatat actttcccag cttcaatcag cggtttcata taacgataaa agaaagtaag
     9001 cagtaatact tgaatatggg cgccgtctgt atccgcatcg gtcatgatga ttactttgtc
     9061 gtaattacag tcttcgatag aaaactcagg accgactcct gcaccaatcg tatagatcat
     9121 cgtattgatt tcttcatttt tcaagatatc ttgcatttta gctttttcgg tattgatgac
     9181 ttttcctcgt aaaggtagaa ttgcttggaa ctttctgtct cttccttgtt tagcagatcc
     9241 gccagcagaa tccccttcga ctaaatacaa ttcatttttc ttagggtttc gggattgtgc
     9301 gggtgtcaat tttcctgaaa gaagagattc gcctttttta cgcttcttcc catttcggct
     9361 ttcttctctg gctttacgtg ctgcttctct cgcttctctg gctttgattg ctttacggac
     9421 caacatctgg ctcatttcgc tattttcctg caaataaaag cccatttgtt cactgatgac
     9481 gttatctact acggtacgag caactggcgt accaagtttt tcttttgttt gcccttcaaa
     9541 ttgcagaaga ttttcaggta cacggataga aagtacagca gctaaccctt ctctgaaatc
     9601 actaccttcc agattcttgt cccgttcttt cagcaaacca acttttctcg catattcatt
     9661 ataagatttc gtcatcgccg ctttcatacc agcttcatgg gttcccccat cttttgtccg
     9721 aacgttattg acaaaagata aaacattttc tgaataaccg tcattgtatt gataagcgac
     9781 ctctacttcg ataccttctt tttctcctga aaaataaaca actggtgtca atgtatcttt
     9841 ttcttcattc agataatcaa caaattcttt gattccttct tcgtaatgaa aaatttcctt
     9901 gactggttct tctccacgaa gatcagaaag agagatttta acgcctttta gaagaaaggc
     9961 agattctctt aaccgttcgg ctaaaatttc atatgaaaat ttcgttgtac tgaatattgt
    10021 atcatctggt aaaaaatgga cagtcgtccc gtttgctttt tttgtttttc ctatcttttt
    10081 caaagtaccg tcaggtttac cgccgttttt aaatttctgc tgatactcta cgccatctct
    10141 aacgatcgtt acaattagcc atttcgataa ggcatttacc acactggctc ctacaccgtg
    10201 cagtccacca gaagttttgt accctccttg accaaacttt ccgccagcat gcagcacagt
    10261 aaaaatcact tcaactgttg gtattccgga agcatgcatt cctacaggca tcccacgtcc
    10321 gctatctgca acagttatac tattgtcttc atggatggtc acatcaatct cactgccata
    10381 acctgataat gcttcgtcaa ctgcattatc gacgatttca tagactaaat gatgcaagcc
    10441 tcggctgtca gtggatccaa tgtacatccc tggtcttttt ctaactgcct ctaatccttc
    10501 caatacttgg atggacgagt cattgtattc gttgtttact ttttttgcca aggcaaacgc
    10561 tccttcaata atactttgcg gcaaaaccgc accacgatta tcatactaga aaaggtcgtg
    10621 aaaaaaaaga gggtagacga gaaatcaatt ttaattatct cctaaccgct ttttcgacat
    10681 tcccctctat cgatcatctc ttctttgtgg tcatttttat ttttggtttt ctttcaactt
    10741 accaagttca atcttaatac aacgatccat tacgatatct gtacgtcctg cttttcttag
    10801 catctcagct gcttctttgt tttcaatccc taattgtgcc cagaacactt tagcatcagt
    10861 tttcaaaaaa tcacctgcta tttcaggtaa aaattcactc cgtctaaata catctacgat
    10921 atcgattgga tcatcgattt ctgtcaaatt cgcatagact ttctcgccta gaatttcttc
    10981 accagccaat aatggattta ctggtatgat tttatatcca ttgtcttgca atacctgtgc
    11041 aaccatgtaa cttgttcgtt ctggtttatt gctcaagcct acgacggcaa tattttttgc
    11101 ttcttccaaa tagttgaaaa tcgtttcttg tgttggattt tttacagaca tttctgccac
    11161 cccttgcttt tctttttatt ccatctatta tacttgtatc tttcattttt tcaattgttc
    11221 ctgttaccta ttcatgctaa aataaaggat agttttcaac ttgaaagagg gtaaaaaatg
    11281 aaattgatct tattacttat tttagcatat cttttaggtt ctattccttc cggtgtctgg
    11341 attggcaaat tgtttttcaa aaaagatatc cgacaacacg ggagcggtaa tactggaacg
    11401 accaatacat ttcgtgtatt aggcaaaaca gccgggattg ttgtcctgct tatggatatt
    11461 ttaaaaggta cattggctac ttgtttgccg atgatcttcc acctttctgg gattaaccct
    11521 ctttggtttg gggtatgcgc cattcttggc catacatttc cgatttttgc tgggttcaaa
    11581 ggaggaaaag ctgttgcgac aagtgcagga atgcttttag gatttaatcc tgtatttttt
    11641 gtttattctt gtatcatctt tatcgttagt ttattttgta caagtatggt gagtttgagc
    11701 agtatgatca gtgcagtatt gatcacttta tctacgatcg tccttcctta tgtagctcct
    11761 gtgattttgg ctaagcccaa ttggttactg acgatcattg cctttttagt ttcttccttt
    11821 atcttttatc ggcataaaga caatatcaag cgtatcagaa accatactga aagtcgaatc
    11881 ccgtttggtt tgaatagcag caagaaaaaa tcctgatact ttttcattcc aaagagataa
    11941 aacgggagtg tgacaaatcg atgtcacact cccttattac ttacaggata gcttttttac
    12001 acaccttggt aaacggtgtt ccctgctaac ttctcggtca caacctctta cttcactgtg
    12061 atggaatatt ttgtcttgaa gatttcatgg gcacctagtt gattgatccc tactttttcc
    12121 atcaagtttc cactactgtt caccgtgtca gcgattccgc accacggttc gatacaaacg
    12181 aaaggcgctt cttttggata aggagaccag atccctacat aaggcatatc tttatagctt
    12241 aaagttacgc tatgggaaga tttttcactt cgaatggcgt acgcatttag accccttgtt
    12301 tcaaaaacca aggcatcttg ctcaaaaagt tcacgattca aagccagatt cgtatttgtc
    12361 tgacctaacg tcttttgatc catatcgatg aatgggcctt tcaatggtag gcgtaatctt
    12421 gatttcaatg gagaaaatgc cagatagaaa tcttcaaatg acaatccttc ttcaagaggt
    12481 acattgaaag caggatgtcc accgatagaa aagaacattt ctttttcacc ggtatttgcc
    12541 acttgataat gaaccgtgat gccgtctcct cctaattcat agctgatcac taattcaaaa
    12601 tcaaatggat acttttctaa tgtctccggc gtactcctta agatgaaact agccatttct
    12661 tgtccatgtt caatgacttc aaaatccata tcccgagcaa atccgtgttg ccccatttga
    12721 tatgttttcc cttgataaac atactgatca tcttttaaac gtccaacaaa agggaataaa
    12781 accggtgcat gtctgcccca atacgctgga tcaccttgcc agatatactc taaaccatgt
    12841 tcttttgatt tcagactgat caattccgca ccgtgttcag cgatttcggc gatcagctgg
    12901 tcattttgaa taataacagt cattgccatt cctccaactt cgacttactt tttaaaatgg
    12961 gaaggctgga acaaacattt ctatctgccc cagtcccctt acttctaata actgtgtttt
    13021 gctaactcgt ctttaaaacg ggaattcaat acttttaaat aagttccctt catgcctaac
    13081 gaacgagatt caatgattcc agctgattcc aattttcgta aagcattgac gatcacagaa
    13141 cgagtgatgc cgatactatc ggcaatatta gaagcagtca gacgaccttc atctccatcc
    13201 aatgcttcaa agatagcttg tacagccttc aattcgctat aagataatgt gttgattgcc
    13261 atttggacag ccgttgcact ccgtacatct gcttcgatgc ttcgagactt ctgataaagg
    13321 atctgcatac cgacaaccgt agctccatat tctgctaaga ctagatcgtc atctgcaaac
    13381 gattgttcca tacgagataa gataatcgta cccaaacgtt cgccagctcc aaagatcgga
    13441 acgacagtcg tcagaccaaa tggatatttc tctcttgact ctaccggaaa tgcagtaata
    13501 tcactagtaa ttgggatatt agcttcagtt ttcatcaact gtgccactgt atcagtataa
    13561 gattcaggaa attgtttctc aacaaacatt gttttgattc gttcattgtt cacatcgtga
    13621 cgttcattat aacctagtaa tactccgtca cttcctatga tataggtgtt gctgtcaaga
    13681 ataccaccta gtgtgacagc cattttatcg taaggcaaat ctgcttgcat gtcaaaagta
    13741 ttcttttgct gcaaaagtgt gtttatcttg cgggttttcg ttagcaagtc tgtcattgtc
    13801 gtcctccgat tttttataaa atatagcgac ttaaatcttc atttgccgca attgagccaa
    13861 gtttgtcatc cacatatgct tctgtgatcg ttatttctcc catttgcata tctggtgctt
    13921 caaataatag atcttctagt agtttttcca aaatggtatg cagtctgcgt gcgccgatat
    13981 tatctgtgtc acggttgaca tcgaaagcga tttgagcgat ccgttcaatt gcttcttttg
    14041 taaacgtaac tgacacattt tctgtaccga tcaacgcgac gtattgtttt actaaagcat
    14101 tatttggttc ggtcagaatc ttcacaaaat cttcagctgt caaatcatct aattctaccc
    14161 gtatcgggaa gcgtccttga agttctggaa ttagatcact tggtttagac aaatggaaag
    14221 ctcctgaagc aatgaatagg acgtgatctg tttggatgat cccgtatttt gtgtttactt
    14281 gtgaaccttc gacaattggc aatatgtctc tttggacgcc ttctctggat acttctccag
    14341 attgctgtga tttagaagtg atcttatcga tttcatcgat aaaaataatt ccactttctt
    14401 ccgctaattt gattgcctca ctatggatat cagcatcctt gactaatttt gctgattctt
    14461 ccttgatcaa taactctaaa gcttctttga ctgttaccgt ccgttccact tttttcttcg
    14521 gtgtcaaagc acctagagtt tcatttaggt cgatccccat ttgctccaat ccattgttca
    14581 tcattggtgc aggtttcttt tggtcttcaa cttcaatggt tacttcacga ctatccagta
    14641 aacccttttg tagttgttcc aagactgtac ggcgattgat tcgaatatct tctgtcactt
    14701 cttcttgcgg ttcagcggta ttttgctgac tagcattaaa aagattcatc atttgttcaa
    14761 attgattagt ggattgtttc tgttcttttt tgataccagg taccaagatt tttaccaaac
    14821 gtttttctgc atttttttta gcacttgtgt atacacggct gtactgctct ttttctacga
    14881 tttggatcgc attttctact aaatcccgaa ccattgattc tacatctctt ccgacataac
    14941 cgacttcagt aaattttgtt gcttcgactt tgacaaatgg tgcattgacg atttttgcca
    15001 aacgtcgtgc gatttctgtt ttaccgaccc ctgtaggtcc gatcatcagc atattttttg
    15061 gtgtaatatc ttgttgcatt ttttcatcta actgcaaacg gcgatagcgg ttacgcaaag
    15121 caacggcaac agattttttt gctgcgtgct gtcctacgat atattcatct aattcactta
    15181 cgatttgtct tggtgtttgt tttaattcat acatatctac tcacctctta cagttcttct
    15241 acgataatat tatgattagt aaagatacag atatctgcgg caatatttaa cgcattttct
    15301 gcaatttctt tcgcactcat gtcgttttga gcatagtttt tcattgctcg ggctgcagcc
    15361 aacgcaaaat tccctcctga tccgatagct aagataccat catctggtgt gataacttct
    15421 cctgtacctg aaacaagaag catttcttta tcattcatta cgatcagcat cgcttcaagt
    15481 ttttgcatag attgctgtgt ccgccattct tgagctaatt cgacagctgc gcgttgtaga
    15541 ttcccgttat attcgtttag tttgccttca aatttttctt ctaaagtaaa cgcatctgca
    15601 acacttccgg cgaaacctac aacaacttct ccattgtaga tacgtcgtac tttctttgca
    15661 gtgcctttca tgacaacaga ttcacccatt gttacttggc catctccagc catagcaaat
    15721 ttcccgtctt tttcgacggc acagatagtg gttgaatgaa atggtgattc gaccatgttt
    15781 gtttcctcct aaatttgttc ttttccttat ttttacgctc ttggatggaa agaacgatag
    15841 tttttttgta aactttcttt tgttacatgg gcatagatct gggtagtcga taaattggca
    15901 tgtcctagca attcttggac tgtccgcaaa tctgccccat tatttaataa atgagtagca
    15961 aatgtatggc gcagcatgtg aggatggata tcactatcca aactgctttt tttgatcatt
    16021 tgattcaaga catattcaat tccagtaggc gtgattggtt ttccatgatg attcacaaaa
    16081 acgatatcat gctcttcgtg atattttgtc atcaatacct ggcggccttc cctgaaatat
    16141 tcttgcagag catcttgagc aaaagaacct agaggagcat atctctcttt gttcccttta
    16201 ccatggatca acatgacctc tgaatcaaaa tcaatgtctg ataccgacaa actgctacac
    16261 tcacttaaac ggatgcctga cccgtaaagg acttccagta aagcgcggtt cctcaaatcc
    16321 aaaggactat ctcctgctac gctgtcaaaa agtgcttgca tttcattttc ataaaaaaaa
    16381 cgtggcagtc tcaaattttt ctttttgaga tgaacgtaag aaaatggatt ttcttttatc
    16441 acttcttctt tgagaagata ctgataaaac gaacgtaggc tggctatctt gcgactgata
    16501 gaatttctgc tatagctagg acgatgatca gctaatgcac tcaaatagac acgaaccgta
    16561 aaatattccg ccttcagcag atcagcgtct ccagattcct ccaaaaaatg tttgaaatgg
    16621 agaatgtctt cttcatatgc ttctttcgtt ttttctgaat atcctctttc gacaatcaaa
    16681 taacgcaaaa atttttgttc ccacgtggct tctgtcattt tctcaccttc cttttgtccc
    16741 agagaataat ttagcacagc gtttataaaa agacaaataa attgtcagaa tttaaccaat
    16801 atttctaagt ttgttcacaa ttggcgcaat tcctcacatt ttgtttgaga aattcttaag
    16861 aaaaacgcaa gaaaaagagc gagactatcc gtctcaccct cttcatggag tttccttact
    16921 catttgggat tgcagttttt tctaattctg tttttattgc ctcattagct tttaaagctc
    16981 gttgtgcaat tgcttcatat cttgcttttt tgtctcgaat gcgctctggc agttcaggaa
    17041 acagaccata gttggcattc atcggttgga aatgttttcc ttccgcatga gtaatgtagt
    17101 aagccatact tccaatagct gtttcttgtg gaaactcgac tggttccatt tcttttgcta
    17161 agcgtgcggc gttgattcca gcaagtagtc cgctcgctgc actttcaaca tagccttcta
    17221 caccagtcat ctgacctgca aagaacagat cttcgcgttt cgttgattgg taagtcggtt
    17281 tcagcaattc tggcgaattc ataaagctgt ttcggtgcat cacaccatag cgaacgaatt
    17341 cagcattttc taacccagga atcatccgga aaacacgttt ttgctcgccc catttcaagt
    17401 gagtctgaaa gcccacgatg ttgtataaag aagctgcagc attgtcttga cgcagctgga
    17461 taacagcata aggacgtttg ccagttttag gatcttccaa tccaactggt ttcataggtc
    17521 cgaaaagcat cgttttgaat cctcgctgtg ccattacttc aattggcata catccttcaa
    17581 aatatttttc tttctcaaaa gatttaagtt ctgccacttc ggcattgatt agtgcctcat
    17641 ggaaagcttt gaattcctct tctgtcatcg gacagttcaa gtaagctgct tctcctttgt
    17701 catagcggga cttcaaatat actttatcca tatcgatcgt agctttgtct acgattggtg
    17761 cagctgcatc atagaaataa aaaccatctg aaccattgaa tgctttgatg ctttctgcta
    17821 atggatgaga agtcaaaggt cctgttgcta ctacggtgat tccttccggt atttcagtaa
    17881 tttcttcgtt tatcactgtt actaatggat gctctttgac ttttcgggta attgtttcag
    17941 aaaaagaatc gcggtcaaca gctaaggcac caccagctgg cactgctgtt tcatctgccg
    18001 aagtgattac taccgaattc aatcggcgca tttcttcttt caataaccct actgcattag
    18061 ccaaactgtt tcctcttaat gaattggaac atactagttc agcaaaatta cttgtgtgat
    18121 gcgcctcagt cgatttgact ggacgcattt catataaacg gacagggact cctgcttgtg
    18181 cgatctgcca agcagcttca cttccggcaa gccctgctcc aacaacattt accattttca
    18241 atgaaagaac ctcttttcta ttatttttgg atgtcttctt cgtaatctcc atttacacag
    18301 actacttgtt taccgccttt gactttcttc tcgaccagat atccgtcaca ttttggacaa
    18361 tttctgccca ctggtttatc ccaagaagtg aattcacatt ctgggaaacg ggaacaacca
    18421 tagaatagtc tgtttttctt agattttcgt tcaatgactt gtccttcatg acatactggg
    18481 caagtcacac cgatttcttt tacgattggt ttcgtattcc gacaatcagg aaaattactg
    18541 caagcataaa acttcccata tttccctaat tttatcacca tcggatgtcc acagacatca
    18601 caatcgaatc ctgctggttc atctttgatt tgaatttttt cgattttttc ttccgcattc
    18661 gtcagttctt tttcaaacgg tttatagaaa cgatcaacca ctttgaccca cgcttccgtg
    18721 ccttcttcaa ttttatccaa atctccttcc atttctgctg tgaaatggat atcgacgatt
    18781 tgagggaaga attcacagat taatgaattg acaatttctc ctagttcagt cggctcaaat
    18841 cttttttgtg tcaattttac atagtagcgt ctttggatcg tatcaagtgt tggtgcataa
    18901 gtggaaggtc gccctacccc gttttcttct agtgcacgaa tcaacgtagc ctcactaaaa
    18961 cgtgctggtg gttgagtaaa atgttgtttt ggttcaatat caacagattg gactacatcg
    19021 ccttcttcaa gttctggaag aatattttct ttctcttctt tgccgtcatc tctaccttcc
    19081 acatacactt gcatgaatcc tttgaatttg atttttgaac cgttcgcaat gaagatgaca
    19141 ccattttgtt ctaatgtcac tttcattgta tctaaaatag ctggtgtcat ctgactagcc
    19201 acaaaacgtg accagatcaa tgtataaagt ttcaattgat ctttatctaa atattgtttg
    19261 agttcatctg gtgtacgaag cacactcgtt ggacgcacag cttcatgggc atcttgtgcg
    19321 ccttgagcat ttttgatctt tcggtgacca tgtctagaaa attcttttcc atacgtatct
    19381 tcaataaatg ctgcggcctc tgcttttgct gtatcggcaa tccgggtaga atcagtacgc
    19441 atgtatgtaa tcaagccgac tgttccttgt ttacccaaag tgattccttc atataattgc
    19501 tgtgctacca tcatggtttt tcttgttcga aagtttaact tacgggcagc ttcttgttgc
    19561 aggctacttg tagtgaaagg attcgcaggg tttcttttgc gctctttctt ttctactttt
    19621 ttaacttcga agtctgttcc atcaattcta gcagtgactt cttttacact ttgtgcatct
    19681 ggtaatttct ttttcttacc gtcgattccc cagaaatttg ctttgaattt cttgcgagct
    19741 tttttaaaat taccgtcgat gctccaatat tcttctggaa caaattctcg gatctctttt
    19801 tcacggtcaa taataatttt taaagcgaca gattggacac gtcctgcact taaccctttc
    19861 ttgaccttac gccagagaat aggactgatc gaataaccaa cgattctgtc taaggtacga
    19921 cgagcttgct gtgcatctac taaatctaca tcgatcgttc ttggttcctt aaaagctgct
    19981 ttgactgcgt cttttgtgat ttcattgaag accacccgat ttttttcttt cagatccaac
    20041 ccaagtaggt acgctaaatg ccaagcaatc gcttctcctt ctctatccgg gtcacttgcc
    20101 aagtaaactt tttgagcttt ttttgctgcg gctttcaggc ttttgatgac atcgcctttt
    20161 ccgcgtatag aaatataatg gggttcataa ttgttttcaa aatcgatacc cattttactt
    20221 ttcggtaaat ctcttatatg gccaacactg gccattactt tatagtttcg tccaagatat
    20281 ttctcaattg tcttggcttt tgcaggtgat tctacaatca ctaaatattt atacgccatt
    20341 actgaatgga ctcctttcca ttgtcgtgtt cctactatta aaagaggatt catacaaatc
    20401 gtagctaatc atatccattc agaaaacgtt tgtcaagaaa agcaaatgaa aagtatggtg
    20461 ttattcttta aattctgcca agatatcttc acccgaaacc gtgcaaactg caccgtcttg
    20521 gatcaaatga tgacatccgg tagaatgttc tgttaaaata tttcctggaa cagaaaaaac
    20581 tgttcttcca agatccaatg cttgctgtgc tgttatcaga gagccgcttt ttttcttggc
    20641 ttcaaagaca catgttcctt tagacaaagc tgcgataatc cgatttctca ttggaaaaca
    20701 atgctttttc gggccggatt gaggaggata ttcactcaaa agaagatgat catttgcaat
    20761 cacctcttgc aagtcacgat tttcagcagg atagacttgc tctatccctg ttccaatgac
    20821 tgcgattgtc cgtccaccat gagagattgc tgcttggtga ccgtatgtat ctatcccttt
    20881 cgcgcagcca ctgacgatca catatccagc ttctatcact ggttttagta aataataaac
    20941 tgtttttttg gctaaagcag aagtgtgtct tccaccaaca acgcttaaca tgttacaaga
    21001 aagtaaagat agatcaccat tataaaacag tacaattggc ggataggtca agtgaagcag
    21061 ctgaggggga tatttgggat caaagcaagt gataaacgac tgtttcgatt ggattttgcc
    21121 ccagttagcg tgaatttcct gccaactttt gcgaaacagc tcccggtgtt tggttatttt
    21181 acctatctta atgatgtctt caaatccgat ttctttataa ggatactgca tcatccagtt
    21241 agcaagtgcc cattttcctt gactactgat tccttttgta taagcaagtt tcaataaatt
    21301 ttcttctatt tgatacatca taaaaccgcc tttcgtctct ctttctaata agatacgtaa
    21361 aagcggctgt tttttattgt aaatattttt ttacaggtgc aaaactttta cgatggattg
    21421 gcgtaattcc ttgtcgttca agtccttcta agtgttcctt tgttccataa cctgcattat
    21481 gcgagaaacc atatccagga taaagcacgt catattcttt catcaattgg tctcgatata
    21541 ctttagcaat gatacttgca gcagcaatcg acacagatcg tgcatcccct ttgattattt
    21601 tttcctgctg aataggcaaa tccaaggtca tcgcatcgat caacaaatag cttggtacaa
    21661 tggacaattt ttctactgct tggatcatcg ctaatttact tgcttcataa atattcacat
    21721 catcgattgt ctgctgatca acgatcccga tcccgattgc cagggctttt tcttgaactt
    21781 gttgataaag aagttctcgc tttttctctg ataattgttt ggagtcattc agccctaata
    21841 tgatctcatt tttcggcaag ataactgcag ctgccacgac aggtccagct aatggtcctc
    21901 ttcctacttc atcaatgccg gcaatccatt ggtgaccttg tgcgtaccct tgcttttcaa
    21961 agcaaaacat ttcttcatat ttttccttaa ttaatgcttc ctttttcaat cgtcgttcaa
    22021 aagaagccag tgcttgctgc acgcctttgc gtggatcatt tcttaatgat tctagtgtag
    22081 gatcatctat tctctgtatg cttttcaatc gttctttgat actttggatg gattcagtca
    22141 tcactttttt gctccatttc ttcgcaacga tccagtgtgt atcgtccaag ttttccttga
    22201 cgaatatcat gcacgactaa ttcgctgcca cgatcatagt cttctttata cccacgtttt
    22261 gctgtaatat ccatcagtaa ttccggtgcc aatttttctt cttgctcgat tgataaacca
    22321 taacgatcgc tgattcttcc tgaataatat cgggcaaaaa agtctaatcc gtaaatcgct
    22381 aaatcatcta aatggacaag ctggtctttg atcgcgcctg tcagcgctag ttttttgccg
    22441 atttcttcat cttcaaattt gggccataaa atccctggtg tgtctaacaa ttccaactca
    22501 ttacctaaac gaagccactg ctgacctttt gttacgccag gcttatttcc tgtctgtgca
    22561 attttcttgc ctactagtcg attcatcaac gtagatttcc caacatttgg aatacctaag
    22621 accattgccc gaatagcgcg aggcttgatc ccccgagcag catctcttgc ccgtttttct
    22681 ttcaatgctt ctttagccgc aggtactaga cctttaattc ctttactttc ttgtgccgtg
    22741 atagctaatg caagatggcc ttgtttacga aaatattctt gccacagctt tgtctgttgt
    22801 ggatctgcca gatccgcttt attcaataaa acaaggcgag gtttttgctg aatgatctga
    22861 tctagcattg gattacgaga agataaaggt aatcgtgcgt ctaccaattc aaatacgatg
    22921 tccacatatt tcattttttc agaaacttct cgtcgggctt ttgccatatg tcccggaaac
    22981 cattggatgg tcattgttct ctccattcct ttcataccgg aatattcccc ggctttactt
    23041 gtttttttct ttttcaatat aaattcactt ttttatt
//
LOCUS       DACDIP010000042        22450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDIP010000042 DACDIP010000000
VERSION     DACDIP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.042"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IV257_001812"
     CDS             25..696
                     /locus_tag="IV257_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8814917.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IV257_001813"
     CDS             755..1195
                     /locus_tag="IV257_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814918.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IV257_001814"
     CDS             1207..1875
                     /locus_tag="IV257_001814"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8814919.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IV257_001815"
     CDS             1939..2562
                     /locus_tag="IV257_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8814920.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IV257_001816"
     CDS             2618..3004
                     /locus_tag="IV257_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8814921.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IV257_001817"
     CDS             3217..4608
                     /locus_tag="IV257_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8814922.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="IV257_001818"
     CDS             5219..5587
                     /locus_tag="IV257_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8814923.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="IV257_001819"
     CDS             5767..6708
                     /locus_tag="IV257_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8814924.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="IV257_001820"
     CDS             6777..7508
                     /locus_tag="IV257_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8814925.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="IV257_001821"
     CDS             7652..9832
                     /locus_tag="IV257_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8814926.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="IV257_001822"
     CDS             9852..10334
                     /locus_tag="IV257_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8814927.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="IV257_001823"
     CDS             10350..11111
                     /locus_tag="IV257_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8814928.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /locus_tag="IV257_001824"
     CDS             11242..12576
                     /locus_tag="IV257_001824"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8814929.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="IV257_001825"
     CDS             12883..13368
                     /locus_tag="IV257_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8814930.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="IV257_001826"
     CDS             13361..14044
                     /locus_tag="IV257_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8814931.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="IV257_001827"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="IV257_001827"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8814932.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="IV257_001828"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="IV257_001828"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8814933.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRQKI
                     AK"
     gene            17660..18289
                     /locus_tag="IV257_001829"
     CDS             17660..18289
                     /locus_tag="IV257_001829"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8814934.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="IV257_001830"
     CDS             18341..18673
                     /locus_tag="IV257_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8814935.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="IV257_001831"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="IV257_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8814936.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="IV257_001832"
     CDS             19499..20872
                     /locus_tag="IV257_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8814937.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="IV257_001833"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="IV257_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8814938.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="IV257_001834"
     CDS             21927..22367
                     /locus_tag="IV257_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8814939.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7612 a         3872 c         4896 g         6070 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa tattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggca aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaat
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt
//
LOCUS       DACDIP010000043        22034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDIP010000043 DACDIP010000000
VERSION     DACDIP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.043"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IV257_001835"
     CDS             156..1862
                     /locus_tag="IV257_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8814940.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IV257_001836"
     CDS             complement(2020..2922)
                     /locus_tag="IV257_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8814941.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IV257_001837"
     CDS             complement(2919..3623)
                     /locus_tag="IV257_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8814942.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IV257_001838"
     CDS             complement(3654..4295)
                     /locus_tag="IV257_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8814943.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="IV257_001839"
     CDS             complement(4333..4647)
                     /locus_tag="IV257_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814944.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /locus_tag="IV257_001840"
     CDS             complement(4635..5264)
                     /locus_tag="IV257_001840"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8814945.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IV257_001841"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IV257_001841"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8814946.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IV257_001842"
     CDS             complement(5984..6916)
                     /locus_tag="IV257_001842"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8814947.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IV257_001843"
     CDS             complement(6913..7689)
                     /locus_tag="IV257_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8814948.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IV257_001844"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IV257_001844"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8814949.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IV257_001845"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IV257_001845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8814950.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IV257_001846"
     CDS             complement(12014..13294)
                     /locus_tag="IV257_001846"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8814951.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IV257_001847"
     CDS             complement(13313..14239)
                     /locus_tag="IV257_001847"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8814952.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IV257_001848"
     CDS             complement(14252..15535)
                     /locus_tag="IV257_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8814953.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IV257_001849"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IV257_001849"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8814954.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IV257_001850"
     CDS             complement(16323..17234)
                     /locus_tag="IV257_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8814955.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IV257_001851"
     CDS             complement(17231..17695)
                     /locus_tag="IV257_001851"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8814956.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IV257_001852"
     CDS             complement(17700..18398)
                     /locus_tag="IV257_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8814957.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IV257_001853"
     CDS             complement(18385..18771)
                     /locus_tag="IV257_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814958.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IV257_001854"
     CDS             complement(18918..20588)
                     /locus_tag="IV257_001854"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8814959.1"
                     /translation="MKNDLQISQETELEPIVEMASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IV257_001855"
     CDS             20771..21004
                     /locus_tag="IV257_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8814960.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="IV257_001856"
     CDS             complement(21103..21534)
                     /locus_tag="IV257_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8814961.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="IV257_001857"
     CDS             21613..22026
                     /locus_tag="IV257_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8814962.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         6236 a         4923 c         3889 g         6986 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccatttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaa
//
LOCUS       DACDIP010000044        21986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDIP010000044 DACDIP010000000
VERSION     DACDIP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.044"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(383..583)
                     /locus_tag="IV257_001858"
     CDS             complement(383..583)
                     /locus_tag="IV257_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8814963.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(888..2120)
                     /locus_tag="IV257_001859"
     CDS             complement(888..2120)
                     /locus_tag="IV257_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8814964.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2374..2943
                     /locus_tag="IV257_001860"
     CDS             2374..2943
                     /locus_tag="IV257_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8814965.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3121..3561)
                     /locus_tag="IV257_001861"
     CDS             complement(3121..3561)
                     /locus_tag="IV257_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8814966.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3719..4483
                     /gene="map"
                     /locus_tag="IV257_001862"
     CDS             3719..4483
                     /gene="map"
                     /locus_tag="IV257_001862"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8814967.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4515..5438
                     /locus_tag="IV257_001863"
     CDS             4515..5438
                     /locus_tag="IV257_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8814968.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5514..6653
                     /locus_tag="IV257_001864"
     CDS             5514..6653
                     /locus_tag="IV257_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8814969.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6646..7446
                     /locus_tag="IV257_001865"
     CDS             6646..7446
                     /locus_tag="IV257_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8814970.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7446..8273
                     /locus_tag="IV257_001866"
     CDS             7446..8273
                     /locus_tag="IV257_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8814971.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8251..8985
                     /locus_tag="IV257_001867"
     CDS             8251..8985
                     /locus_tag="IV257_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8814972.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9085..9951
                     /gene="rfbA"
                     /locus_tag="IV257_001868"
     CDS             9085..9951
                     /gene="rfbA"
                     /locus_tag="IV257_001868"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8814973.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9965..10537
                     /gene="rfbC"
                     /locus_tag="IV257_001869"
     CDS             9965..10537
                     /gene="rfbC"
                     /locus_tag="IV257_001869"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8814974.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10559..11587
                     /gene="rfbB"
                     /locus_tag="IV257_001870"
     CDS             10559..11587
                     /gene="rfbB"
                     /locus_tag="IV257_001870"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8814975.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11685..12536
                     /gene="rfbD"
                     /locus_tag="IV257_001871"
     CDS             11685..12536
                     /gene="rfbD"
                     /locus_tag="IV257_001871"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8814976.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12571..14604
                     /locus_tag="IV257_001872"
     CDS             12571..14604
                     /locus_tag="IV257_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8814977.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14648..15928
                     /locus_tag="IV257_001873"
     CDS             14648..15928
                     /locus_tag="IV257_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8814978.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16138..16944
                     /locus_tag="IV257_001874"
     CDS             16138..16944
                     /locus_tag="IV257_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8814979.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16956..18176
                     /locus_tag="IV257_001875"
     CDS             16956..18176
                     /locus_tag="IV257_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8814980.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18166..19752
                     /locus_tag="IV257_001876"
     CDS             18166..19752
                     /locus_tag="IV257_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8814981.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19791..21929
                     /locus_tag="IV257_001877"
     CDS             19791..21929
                     /locus_tag="IV257_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8814982.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6940 a         3720 c         4561 g         6765 t
ORIGIN      
        1 tagagattga ttaataagct atcttcgatt gctaaaaaga aaacttctga attccatact
       61 ttataaaaat ctttgataaa tctattcgaa gggtcaaatt taaaccataa ttgtgttttt
      121 ccttccatca gcataaaagt tcacctcaaa aagagtatac gaacgaatgt tctttttgtg
      181 aaggcggaat ttaatatact gaataataga aaacaagtac ttgtgccaaa ttatgtgcca
      241 aaaaaattcg aattcaacaa aatgtaaaca aatagaaaac cttctatatt aagatttcct
      301 gcaataacaa acacaacaga agacgtgtaa tagttagtca ggaacgtaca aataacccct
      361 gtatcctttg cggtacaggg ggttattttg tataagtaga cattgtagtt tctaaactgc
      421 tatgtcctaa acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg
      481 catgcgtatg tcttagtcca tcaataaaca gtcgaagaga aaatagggaa aaattgatta
      541 ctaaagaaaa acttactagg tcgacggtgt agttgatttt catacttcaa tagattcgaa
      601 ttaaacgatt tcgattggct tataccagtc ctatccaacg aaagatatat cgttcttaac
      661 tctagcggta taacttgaag ctttacgtaa agccaatggg aatgaactgg agatgacttt
      721 ttattatttt tagtaatagc tgatgggtta atacttacaa ctttcctttt caaagtacca
      781 tataaaaaaa cggtggggtt gtttagcgaa ataaaggatc taagccaata gcttagatcc
      841 tttattctat gctttatatt attttttgca tgtgtgcgaa agtatactca agcccaatcg
      901 ccatttcgga aaatcggtac tgtagagcca tcttcacgga ttccgtcgat atccatcttg
      961 tcagatccta ccatgaaatc tacgtgggtt tgacttctat tcaatccagc ttctgctaat
     1021 tcttcatcgg acatttctgt accgcctttc acactgaagg cataagcaga acctagtgct
     1081 aagtgattcg atgcgttttc atcaaataat gtattgaaaa agatgatgcc agattgagaa
     1141 ataggtgaag ggtcaggtac aagtgcgact tctcctaaac gacgcgcacc ttcatctgta
     1201 tcaagcagtt ttgctaagac atcttctcct tgttcggcag aaaaatcaac aacttttcca
     1261 tctttaaaag taaatttcat acccgagatg atggtgccag cataactcag tggctttgta
     1321 cttgaaatgt aaccatctac tcgatgactg tcaggtgctg tgaacacttc ttccgtcggc
     1381 atatttgcca tgaatttttc tccacgagca ttatagcttc cagcgccttc ccaaagatgg
     1441 tttttaggca atccgataat gatatctgta ccgggagcag tatagtgaag ggcagaaaat
     1501 tgttctcgat tcaattcttc tgcttttttt gctaattttt catcatgctt cttccatgct
     1561 aagacagggt cttcttcata gacacgagtc gttttgaaaa tctgatccca taatgcctca
     1621 acttgttttt cttctggcag ttctgggaag acttttgctg cccattgttt accagccgca
     1681 gccacgacag tccaactgac tttatttgct tgagttgcct tgcgtaaatt cattagtgct
     1741 ttgccattcg ctgattgata agacgctact cgatggctat ctacaccagc aaatgcatcg
     1801 ggatcggcag aaacaacgct gatcctgctt gctccttttt caagccattc atccgcttga
     1861 tcgattttag attgtggtac attctcaata cgatccgttg cagcatgtaa aagaaattct
     1921 ctttggatct ggtcgtcagt ccattgaacg ataacctcag cggctcctag tttataagct
     1981 tcttgtgtga tcaatcgagc aaggggtgcc tgttccacgc tgatctgtaa aacaactgtg
     2041 tgtccttttt ctgtggctac gccagtttct gcaattaagc gtgcatattt ttttagaagt
     2101 tcattaaaat cagataacat aataaaaatt cctcctcacc attttttctg gtaactatta
     2161 gcatgatacc atttaattat gattcaaaca atgaggcttt aaataaaaaa aggaggttat
     2221 ttttttaagg ttaagtttca tgatatatgt tactacttgc tatttcagct tgtgaagaaa
     2281 atcaaaaact aattttgcat atcaaaaaaa catgttacaa tgaaattaat caatggtgga
     2341 agcgttataa aaaaattaag gagattaaaa aatatggcaa gaaaaaagac gattactaga
     2401 gaacagattt tggcagctgc ttatgaagta gctgccaccg atggatttac acgatttact
     2461 gcgagaaaca tcgcgaacaa aatgaattgt tccacgcaac cgatttatct tgagtttaaa
     2521 aatatggatg acttgagaca agctcttttt gagcagatct atgatcattt acaatatgaa
     2581 gtgttcactg ttgagcatac agggaatacg attatcgatc ttgcttttaa ttatatccat
     2641 tttgcaagaa gagagaagaa gttgtatcgt tcactgtact tagaagaata tggtggagga
     2701 aaagagatgc agaatttctc ttatacttac tttgtgaata cagtgaaacg cgatccagct
     2761 tatgtgaatc tttcagaaga gcagatcgaa tctttgtata atgggacatg gatcatagct
     2821 actgggatcg cagctttgat gtcttcaagc atcatccatc caactgatga acagattgaa
     2881 aagatgatcc aagacagtat agatgctatc ttgaaattca aagaaccaat cgaaatcgat
     2941 tgaatatact caaaagttcc acaaatcagt aggtaacaag agatgtgtca aaataaggaa
     3001 aagttcgttt ttcttacagc gtatgcattt ttaactcccg aagaactctt ccatgaatgc
     3061 aatgaataaa agagccatac agattgaata aatctggatg gctcttttat tgcaagtata
     3121 ttaaattttt gcgactagtt ctttagcgaa agcttctaaa ttttcgatat cttcttcttc
     3181 agcattcaga tccactttaa cattttcggc tccttttgat gcgcctgttt ttgcaaaagc
     3241 agcatcgaaa tcatccaccg atttgcagaa ttcatcgtaa aaagtatcac cagatccgca
     3301 gacaccgtaa atttttccca atagatccaa ttcttgcaag tcttcataaa agtctacgat
     3361 ctcatcaggc agatcgccat catcgtatgt ataagtagct actacgcaaa tatctgcctc
     3421 ctcgaattca tctgcatcta cttgggtaca ttcgttgatt tcgacttcga tatccagatt
     3481 ttcaaatgct tctgcaacaa tatcggcgat ttcttcagtg tttcctgtca tgctagcata
     3541 tacgattttt gctaaagcca tgatgtgcct cctcgtttat aattatttaa caattatgaa
     3601 ttataccaaa acatcattga aaagaaaata aaacctaaag acacctgggg aatgtcattt
     3661 ggctaaatgt atgccgctat gttaaaatga gagggatttg taaaaaggag acgaaaatat
     3721 gattacttta aaatcagcac gtgaaattga aatgatggca gaatctggag ctttattagc
     3781 agatgtacat aaaaatctgc gtgattttat aaaaccagga atcacaagtt gggatattga
     3841 ggtatttgtc agaaactata tcgagagtca tggcggaatt gctgcgcaaa ttggctttga
     3901 agggtatgaa tatgcgactt gtatcagtat aaatgatgaa atttgtcatg gattccctcg
     3961 taaaaagcct ttgaaaaacg gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg
     4021 cggcatttct gattcttgtt gggcctatgt agttggagaa tctacacctg agatcgaccg
     4081 tttgatggaa gtaacgaaga aagctttgta catcggtatt gagcaagcac aagtagggaa
     4141 tcggattggt gacatcggtc atgctatcca aacttatgta gaaggcgaaa atctagcgat
     4201 cgttcgtgat tttattggtc atggcgttgg tcctacgatt catgaagaac cagcagttcc
     4261 tcattacgga gaagctggaa aagggttgcg gttgaaagaa ggcatggtta tcacgatcga
     4321 acctatggtt aataccggaa cttggaaaat gaaaatggat cctaacggct ggactgcgta
     4381 tacaagagac ggcggacttt cttgccaata tgaacataca ttggctatta ctaaagatgg
     4441 tccaagaatt ttgacatcac aaggagaaga aggcacttat tgatctatcc agtcaatagg
     4501 tacgggagag gtacatgaaa gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct
     4561 ttattgagac gactcaaaag cgcatcactg attcagaaat gagtacgacc tctgtcgtcg
     4621 ttgcttatta tttactcttg tctttatttc cattgattat tgcttttggt aatattttac
     4681 cgtttctcca catcgatcaa gagactgtac tgacctattt acgagaagtg attcctgaga
     4741 ccgtttacca gtttatcggt cctgcaatca aagacctttt gacacaaagt tcaggaggct
     4801 tgttatccgt ttctgcatta gcggctttat ggtctgcaag tcaaagtatc aatgctttgc
     4861 aaattgcgat gaataaagcg tatggcgtag aaaatagaaa aaattttttg atcgtccgtt
     4921 tcttctcact aattgtcatt gtcttgttca tgattgctat tagtggggta actatcgttc
     4981 ttgggttggg acagctgatt ttggatgcta ttcagccaat cgtacaaata cccgtgaact
     5041 ttatcgatca gttccaaact ttgaagtggc caatcacatt ggtagcttta tttgtcatca
     5101 tgttcttgat ttatttaatt gtccctaatg ctcagttgaa gttgaagacg atcattccgg
     5161 gcacgatctt cgctactgtc ggctggatgc tgttatcaca agtgtttagt atctatgcta
     5221 ggtattttgc aacgaggatc agcggttatc agatcatagg aagttttatt gtactcatgc
     5281 tttggcttaa ttttgctgct acgatcatca ttttgggcgg aatcatcaat gcagttgttc
     5341 aagaatacgt gacgggtcat caaatcaaaa aacgtcgaga tccaagccga aaatggttca
     5401 ataaattgaa aactaaattt tctcataaaa aaaagtagaa acaagagttc ttattgtaaa
     5461 tttatatcag taatttataa taattatgac tcttaattaa agtaggtgcc catatgcttg
     5521 tttcttttag tatggttata agattttttc tgacaatttt attatcactg atattaacac
     5581 ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat gaaagaagaa
     5641 taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca tttgttatat
     5701 cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg atcttgattg
     5761 cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg acacctaaac
     5821 aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca gatatacgta
     5881 ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt agttttcccg
     5941 taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac ggtttggatg
     6001 ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt ggatattttt
     6061 tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta gcaagtatta
     6121 tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat acaggagctc
     6181 tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac gtgacttttg
     6241 tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg ttttttgcta
     6301 tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa catcttcatc
     6361 accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt tatgcgatgg
     6421 ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc ggaacgatca
     6481 tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt ggtctgatca
     6541 atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa gagtaccgta
     6601 cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa taacaaatta
     6661 aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt tcggacacta
     6721 gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat ttttgacaac
     6781 aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc ctacatcgat
     6841 atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa tctgatagct
     6901 agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga agagacagtt
     6961 acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc acctaagatt
     7021 cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt ttttgattac
     7081 gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg actggctgtg
     7141 tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat ctcaggtagt
     7201 tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga gcgtttcttc
     7261 atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta caagttattg
     7321 tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg aaatgcaaaa
     7381 ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg gtggcgtttt
     7441 ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta ttcagtcaat
     7501 ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag cttattattt
     7561 acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat atcgtttatc
     7621 atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg tctcaagcag
     7681 atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc gacgattatt
     7741 ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt ccgatgattt
     7801 tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat cgtacagctc
     7861 aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat tcaggtgttg
     7921 cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt tctttagatt
     7981 ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg gagatccttc
     8041 ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca agtaatcgat
     8101 accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa aggcatttat
     8161 ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc gtcaaaaacc
     8221 ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg tgaattcgtg
     8281 cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca gttagatagt
     8341 attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg gtcagtagat
     8401 gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca gcttttttcg
     8461 gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac gaaaggagat
     8521 ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa aaagatcact
     8581 gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat cattgtggac
     8641 agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa agaaggatgg
     8701 cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg gtcttcttta
     8761 aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg gattggtttg
     8821 ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg aagacatgat
     8881 ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc ttggcgtgtt
     8941 catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa gatgtttaaa
     9001 tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag tgaaatttga
     9061 aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg cagcggaaca
     9121 cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta tgacaaacca
     9181 atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat tctgattatt
     9241 tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa tgatttagga
     9301 ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc gtttatcatt
     9361 ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa tatttactat
     9421 ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg cgcgacagta
     9481 tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga tgagaatatg
     9541 caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc tgtaactggc
     9601 ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc ttcagaacgt
     9661 ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa actatccgta
     9721 gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc attgcttgaa
     9781 gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc ttgtttggaa
     9841 gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct tgcacagcca
     9901 ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata aggagtcctt
     9961 acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa tggacgtctt
    10021 tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg cagaacatgg
    10081 attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg gtgtccttcg
    10141 ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg tagtcactgg
    10201 agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg gtcaatggga
    10261 cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag gatatgccca
    10321 tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata attactataa
    10381 tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg attggccaat
    10441 tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat taaaagaatt
    10501 tgaagcagaa aatccatttg tttacggaga aatctaagaa ttaaggagaa attttcttat
    10561 gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg ttcattacgt
    10621 ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt atgcaggaaa
    10681 caaagagaac ttggccggac ttcctagcga tcgcgtagaa ttggtcgtag gagatgttgc
    10741 agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc attacgcggc
    10801 agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa cgaatatcat
    10861 tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc atcacgtatc
    10921 tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac atggggaagg
    10981 cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat attcttctac
    11041 aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct tacgtgcaac
    11101 catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat ttattcctcg
    11161 ccaagtaacg agtatcttaa gcggtattcg tccaaaatta tatggcgaag gaaagaatgt
    11221 tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt tgacgaaagg
    11281 cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata agaccgtgat
    11341 ggaattgatc ttagaaatga tgggccaacc aaaagacgca tacgaccatg taaacgaccg
    11401 cgcagggcac gatttgcgtt atgcaatcga ttcgacaaaa ttgcgagaag agctaggttg
    11461 gcagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat ggtacgaaga
    11521 acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg ccaaaaatgg
    11581 acaataatac agaggaaatt tcactgtatt aatggaaaaa cacatgtgag gcaggcgctc
    11641 gcctgtctct tttttaaaat aaaaatcaag gagcggataa ccatatgttt ttattaactg
    11701 gaggaaatgg acaattaggt acagaattgc gccatttatt agatgaaaaa aatctgccat
    11761 atgtttcaac agatgcaaaa gacttggata tcacagatgc tgagaaaaca atggcttata
    11821 ttacagaatt aaaacccgat gttattttcc attgcgcagc ttacacagca gtagataaag
    11881 cagaagaaga agcaaaagaa ttagatgaga aaatcaatgt cgatggcaca agaaatgtag
    11941 cacttgcagc aaaagctgtg aatgcacaat tcgtctatat cagtacagac tatgttttcg
    12001 atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca ctaaatgaat
    12061 atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa gactattata
    12121 tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc acgatgcaac
    12181 gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg cgaccaactt
    12241 ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca ccatttggta
    12301 tttatcattt gtcgaacgaa aatagctgtt cttggtatga gttcgcaaaa gaaatattga
    12361 aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa aaagcaacac
    12421 gtccgcagta ttcagtcatg agcctgaaaa agacagaagc gttaggtttt gtcataccaa
    12481 cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat aagtaatttc
    12541 ctttttatca aatagaattt ggagcatgat atggaaaaac aaacaagaag tgcacgacga
    12601 aaagaaaagc caaaagcgaa tcaggcagat tcgaatttgg tgcttgacct gtttctaaaa
    12661 ctccgctatc ttattggcgt tttagtcatc gtggtcatcg ttacttttaa tttaaacgga
    12721 agttcacttg gtgtttggga taaatatgtg tcgcaaagag acgatgggaa aaaaactgat
    12781 gtgatttttg gacagaatcg ggaagtgcgg tcagacgaat ggctggtgca gacacctttt
    12841 tacctctcac aagctgaaga ggacttttcg ttggtgaacg aggattattc attaaacgga
    12901 caaaacatga tcattgcgta taattctcct gtaaaggata ttacggttat cggaaaacca
    12961 ttcaactggg gattcttttt cctcggaaga gatcgaggac tttcctttta ttgggcgatg
    13021 aaattagtcg tcatggtctt gcttggattc gaagtactga tgatattaac gaagaggaaa
    13081 aaagctctat cgctaatcgg tgcatttgcg ctgacatttt ctccagcagt ccagtggtgg
    13141 ttcatgcaac atgtgggcga tttgattttc tttactttgg gactgatgat tgctttttat
    13201 cattatttct acaatcatga taaaaaatgg ctacgaacgt tgatgatgct tttggtggtt
    13261 attttcggta ttgggtttat tttagtgatt tacccagccc atcaagtgat gctggcgtac
    13321 ttgctgattt tttattttgt tggcctgctt atttactata gcaagaaagt ttcatgggat
    13381 ctgttggatg tagtgttgat tggaggggca gttttattta ttggcggagt catggttcat
    13441 ttctggctaa cgtcaaaaga cgcattgtta gcttctttga ataccttata tccaggaaaa
    13501 cgggtaagta ctggaggaaa gtggacgatt ggcgaatttt tttctttcct tacaaattgg
    13561 aaaattcctt ttgaagatat caactattcg aataactctg aagtggccct gttctatcac
    13621 ttttttccaa ctgtattcct agcaagtcca tttgtgttat ttggcaaaaa agatagcgaa
    13681 caaaagttac tgggacgtat tttgatgatt ttttgtctgt tttctatctt ttggattacc
    13741 gttgggttgc caaaatggct agcagaaatc actctgctgt cttatgttcc tccattaaga
    13801 gccatcctta cttttagttt tgcggcttgt ttattatcga tctggtttat cgcttatatt
    13861 tggagagaag atgttattcc tatttggtat aaattcattt tgttggcagt aaatgcatgg
    13921 tcttatttct atgcgattac gcattcgaaa atggataatt atttttcgga ttttgaaacc
    13981 atcgcagttg ctgtgttggg aacagtgatt ttagcttttg cattatttgg ctggagacgc
    14041 ttgttttacc tcgcttttgt cgccttagtt atcgtatctg gctttttcgt caatccagtc
    14101 gtagaaggta caggagcgat ttttgaaaaa acactggcaa aagaaatcca aaaaatcgat
    14161 caacaagatc ctggtaaagt ttggttgtct gaagatgaat tatataactt caccccaaca
    14221 ttgggagtga aatccttcaa tacagtacgc ttttatccag atatagctgc atggaagaaa
    14281 atcgatccga aagaagaata cgagaagtat tataatcggt atgctcatac aagagctttt
    14341 atcgctaagg aagatacaaa gttcatttta gatcgtccag atatgttttc ggtcacgttg
    14401 aattttgaag acgcagaaaa attagggatc gactatgtga tcagtaaacg accattaact
    14461 gattacaacc aattatatga agcgcagttc acgcagctat atggaccgga taaagacggt
    14521 tatatgattt ttaaagtaga atatccggat tatccagatg taccacaaac acaaactcag
    14581 gaaaatcagc tagtcgtaca gtaataggct agctgagaat aatcctgaaa tattagtaga
    14641 aaggagagtg aagataattc aagcaatcgt acaaaaattt gtcaacaaaa tgagtgtcgg
    14701 tttcttgctt ttgttgctga cgctagtctt ttttgtttgg acagcagggg taggttttcc
    14761 agcaactttg tttatatata acaatagttc attacttatc gcttttagtg tggctatttt
    14821 gctcatttta aatgtttata aagcaaaagg cgtcgatttt ctttatctag gtgttgcgct
    14881 tgcccttttc attttctttt cttactttcg ggaattaaga aatgagtctt tatttggaga
    14941 cttactgttt cctttgatta ttttattttg tttagtaatt aaatggatcg agtggacaaa
    15001 atgggatcgc ggtatttatc tgattgtctt tacggtcttc ctcggaatca cgttatatcg
    15061 tgtatttaca gagatccaag taccagaagg acaaagcatc tgggctccag gcaattcgct
    15121 ttctgatatt tggatcaata cgaatacaat cggaagttct ttgatgactc ttgctttatt
    15181 gatcagtgga tttgccagtt cttttgaacg atggtatgtt cgaatcatcg gtgggatagc
    15241 agtcgttgca gcctttttag gtatttgggt ctgccaatct agaggtgcat tagtcgcgtt
    15301 gattgttttt gcgatattgg atcttttacc taaatcgttg atgcgtgtca ttcgtgcgcc
    15361 atttattgcc tacacggtta cgattctttt agctttgccg atttcttatc tggcggctgt
    15421 ctctgaaaaa gtcaatttgt ttaccggtcg agaagatatt tggcataaat tctatcagac
    15481 gctaggagaa aaatctgaac aagttttact aggaatgaaa acatttatct tccagcgagg
    15541 aaaccaattt ttaggaaacc acaatagtta taattctatt ttgaatatct atggattgat
    15601 aggatttggg attgctgctt tgctgcttat cctttttatt ggtcgcttaa cgttgaaagc
    15661 agatttatcc aacgggcaga tgacttttat ctgggccttt tttgcggtga tgatgcagtc
    15721 atttatggag gatacattga cttcataccg atgggttccg attatttata tcctgttggc
    15781 tatggccgtt catcgatatg atgaaccaga agttacggag gaattctatg ttatggaaga
    15841 ggcatatgac gatatgactt atgatgatac atcttatgag gaacaaccag atagcgctca
    15901 ttcttccagg atccaacgct atcattgata ggcacaaaat acgagagagg ctgtgacaac
    15961 cgtcgcagcc ttttttggtt tattgcaatt gtctagcatc ttctgtaaaa tagaaacgtt
    16021 agagaaaatc ttgtatcata gaaaattaag aaagaaataa gaacgcagct cggtaccaaa
    16081 aatatggtaa aatggcactt gactgtaatt cactgaaaat cgcttggagg aacacaaatg
    16141 tttaaagatt tgtatttatt tatgaaggac atttatcaga acagaaaatt actgctacag
    16201 ttttcgatca atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg
    16261 tttatcaatc cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa
    16321 gcacaaatga tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc
    16381 tggttcttct tctcagatgc atttgcatca actacggttg tttttcggga atatacttat
    16441 ttagtaaaga aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat
    16501 ctgtacaccc atttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata
    16561 tatccttcag caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg
    16621 acaggattga cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta
    16681 attacgatcg tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag
    16741 tttgattcag gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt
    16801 tatcgtgagt catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat
    16861 ttctgggttt tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa
    16921 ccacattttt cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa
    16981 aagatatcac aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc
    17041 tcaatccttt taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag
    17101 aaataaaaaa aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa
    17161 tgttgaaaat cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa
    17221 aaatctcagc tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa
    17281 atatctattt aaatggaatg gttctaggat tttcaagaga agaagtcgat gaaatggtcg
    17341 atgatatcat tgaatttgct gacatcggcg accatattta tcaacctgta aaaacctatt
    17401 caagtgggat gttcgttcgt ctagcttttg ctgtagcgat caatgtgaat cctgatattt
    17461 tgattgtgga tgaagcattg gcagtaggag atcttgaatt tcaactaaaa tgtatggaaa
    17521 aatttacaga aatcaagaac tcgggaaaga cgattttatt cgtttctcat gatattagtt
    17581 cgattcgccg tttttgtgat cgttcctatt ggttgaaaaa tggaaaagtt gtcgaatatg
    17641 gtgatacgat ggaagtaacg acgaattacg aaaacttttt gaagaaaaaa tcagttaaaa
    17701 cagtagatcg aaataaaaac caagtagaac gcttcgcagc tccagatatc gtagacgtga
    17761 cgaaagccga attgttggat aaagataaaa atccagtgga tatgctggaa caaggagaaa
    17821 aactatacgt taaagtaacc tatgaagtaa aagacgatac aatcaaaaat ccagttttag
    17881 gaattgctct gcgtacagtc gacaattttt atgtgtgcgg actcaatact ctactagatg
    17941 gtatcaagat cccttggaaa aaaggtgaga atatctttta tctggagtat gaaaagttag
    18001 gtttgttagc tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc
    18061 ctttggtata caagacaaaa tacatgaact tgtttgtcag cggttcttat atcggagaag
    18121 gcatcgtggt cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt
    18181 gaaatggacc tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga
    18241 agtaaagttt tagagtttgg tcctggaaac ggccgattaa cgaaacattt gatcggagca
    18301 aaacaatgtg aagtgagcat tgtcgagcta gataaggaat tgttcgattt tgtcagcgag
    18361 tttgcccaag atggatttta tggagatatc gaaagttttg aatgggcaaa ctattatgct
    18421 ggacaaacat ttgattacgt tttgtttgcc gatgttttag aacatttggt agatcctgga
    18481 aaaacgttga agaaagttag agaatttttg aatgaagaag gagaaattct aattacattc
    18541 cctaaccttg cgcataattc tgtcatgatt gatttgttca ataatcaatt gccatgggct
    18601 tcttatggtt tgctagatga aacccataat tctttttata ctcatgatgg cttccaaaaa
    18661 gtttttgaaa aagcaggatt atacatcaat attgaagatt atttgtactt agctgtaggt
    18721 gatacagaat taaaatcaac ttatgaagaa ttgccggaag ctgtacgtta tgatttcaaa
    18781 atgcgaccat ttggcgaagt ctaccaatac ttcttctcat tgatgaaaca tccagtagca
    18841 caaagttcca ttgcgcagcc gcaaaattct aattatgtga aagtattaga agtaatccag
    18901 caaacgaaac aagatgaaat aattcaacaa ataccattca ataatttcac aggggaaaat
    18961 gagacactaa gtttccatgt atcagaatta gcagaacgaa tggtgtttcg cttcgcgcag
    19021 caacctagct ttatcgaatt cagcgcagaa gctgctggag aaaaactcac atttatcgat
    19081 tcaaatgctg tagtcaaaac agtaaatgat tgttatttgt ttgatggtaa agatttacca
    19141 gagttctttt taacggatat tgcagggaaa gaagtgacta tccattgtca ctatcgtttt
    19201 atcggagaat tgacaccaac aatgaaagaa ttgctggaaa caatccgtcc aatggctgaa
    19261 gttacaaagc agctttctga gaaaaatgat gaattagaaa gagaaaatca ttatcttatt
    19321 gagaataata caagattaga tcagacgttg aaaacaacca taaatcgcta ttgcacgttg
    19381 ttggaaagtg atgaatggac aatcaaacat agattagggc gacgtaagaa agaaacatcg
    19441 aaaaaaatcc aagaaaaaga gctttctcta tgtattgacg aaaaaatctg ggatgcagaa
    19501 acaaagatat tgaaaatcat cgggtgggga atcgctaatt cggatcgcca accattgtcc
    19561 tataagttat cagccgacca atcaccattt tttgaagcaa ttcctgtttt acgagaagaa
    19621 gtcaatcaag caaaacagct agctgaagga gcaaaagcag gatttgaact gcggatcctt
    19681 tgcgagaagg aacgttcatt cttagttgaa gcagtggctc aaaatggtca atcatggatc
    19741 attgaaatgt aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa
    19801 aagaaattgc cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta
    19861 ttgtgggctg ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag
    19921 aaaatgtgat tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc
    19981 ttgatgttaa gacccaaagc ggttttaagg tacgtttatc agggaaaatg agaggaaaag
    20041 caatcttaga cttccaaaca gcaagacatc aaaatggcat agctgtgaaa ttaaacggta
    20101 aatatcctta tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa
    20161 aagggataaa ttatgcaagg actcatggcg taaaaaaagc aatccgccgg gtcaaactgg
    20221 aattgaaacc aggatcgatc gactatgcag agtggatcag tcgtcatgag aaacctgatc
    20281 tgaaagagca aaggaaagaa gtacaaggat ttgcctaccg cccattgatt tccatactta
    20341 tgcctgtcta taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtacttgatc
    20401 aaacgtatga tcactgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc
    20461 gaaagtgtct agaatcctat caagcaaaag atgatagaat taaagtcgtt ttccgacaag
    20521 aaaatggaca catcagtctg gcaactaatt ctgcattgga aatggcagaa ggagagttta
    20581 tagcgctctt agataatgat gacgaattac ctccatttgc cttatatgaa gtggcaaaag
    20641 tgctaaacgt acatccagaa ctggatttga tctacagtga cgaagataaa atcgatgcag
    20701 atgggaatcg ctttgacccg cacttcaaag cagactggtc tcccgataca ttgatgggaa
    20761 ataattatat ctctcactta ggcgtatatc ggacaagcat tgttaaggaa cttggcggtt
    20821 tccgtaaagg atatgaaggc tctcaagatt atgatttggt tcttcgtgtg acagaacaaa
    20881 ttcctgcaga acacatttac cacattgacc gtgtcttgta tcattggcgg acgatcccgg
    20941 gatcaactgc aagcaatgga gaagctaaaa gctacatcta tgattctgga gtgaaagcat
    21001 tgacagatgc tctatctaga agaaatatca agggaagcgt acatcctggc agaatttctg
    21061 gtttttatga aatcacttat gatgttttgc aagaagactt ggtcagtgtg atcattccaa
    21121 ctaagaatgg ctatgaagat ctaaaaactt gtgtggattc gatcatcgaa aaaacgtctt
    21181 atccaaatta tgaaatcatt attgcagata atggaagtac agatcctaaa atgcaagaat
    21241 tatttgcaga atacaagcat cagctgaaag accgatttat cgtcgaatta attgacattc
    21301 cattcaatta ttcacgtatc aacaatctgg cagcagaaaa agcaaacggc aaatactttt
    21361 tattcttgaa taacgataca gaagtgattg aacctgactg gatgacagcg atggtttctt
    21421 atgcacagtt tgatcgaatc ggctgtgtag gagcaaaatt attttatccg gatgatacaa
    21481 cgcagcatgc aggtgtatta ttagggatcg gtggcgttgc aggacatgca ttgaataact
    21541 atgatcgaac acattgtggt tactttggcc gattggtcat tgacgtcaat tacttagcag
    21601 tcactgcagc ttgtatgatg gtcaaggcag ctgacttcaa tgctgtcaat ggatttgatg
    21661 aaatattaga agtagcattc aacgatgttg acttgtgctt gaaagtttat gaactgggtc
    21721 gttacaatgt ctatgcacat caagcagaat tgtaccattt cgaatctaaa tcaagaggtt
    21781 atgaagatac accagaaaaa caaagacgtt ttgctgggga aatcaaaaaa atgcaagata
    21841 agtggccttc ttatattgct catgacccat tttataacga taatctaact aaagaaggaa
    21901 tcggcgattt ttcgttgaga cctgattgat taaaagaact gtattttcat ttttattaat
    21961 gaaaatacag ttcttttaat ttaatt
//
LOCUS       DACDIP010000045        21778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDIP010000045 DACDIP010000000
VERSION     DACDIP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.045"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            127..3654
                     /locus_tag="IV257_001878"
     CDS             127..3654
                     /locus_tag="IV257_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8814983.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IV257_001879"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IV257_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8814984.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IV257_001880"
     CDS             7442..7783
                     /locus_tag="IV257_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8814985.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IV257_001881"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IV257_001881"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8814986.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IV257_001882"
     CDS             9140..11560
                     /locus_tag="IV257_001882"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8814987.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IV257_001883"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IV257_001883"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8814988.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IV257_001884"
     CDS             12994..13731
                     /locus_tag="IV257_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8814989.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="IV257_001885"
     CDS             13745..14560
                     /locus_tag="IV257_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8814990.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="IV257_001886"
     CDS             14573..15220
                     /locus_tag="IV257_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8814991.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="IV257_001887"
     CDS             15236..15892
                     /locus_tag="IV257_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8814992.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="IV257_001888"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="IV257_001888"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8814993.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="IV257_001889"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="IV257_001889"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8814994.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="IV257_001890"
     CDS             18234..18752
                     /locus_tag="IV257_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8814995.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="IV257_001891"
     CDS             18859..19359
                     /locus_tag="IV257_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8814996.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="IV257_001892"
     CDS             complement(19639..20559)
                     /locus_tag="IV257_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8814997.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="IV257_001893"
     CDS             20724..21737
                     /locus_tag="IV257_001893"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8814998.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
BASE COUNT         7300 a         3769 c         4657 g         6052 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgtgcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaat
//
LOCUS       DACDIP010000046        21427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDIP010000046 DACDIP010000000
VERSION     DACDIP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.046"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            312..620
                     /gene="rpsJ"
                     /locus_tag="IV257_001894"
     CDS             312..620
                     /gene="rpsJ"
                     /locus_tag="IV257_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8814999.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            651..1280
                     /gene="rplC"
                     /locus_tag="IV257_001895"
     CDS             651..1280
                     /gene="rplC"
                     /locus_tag="IV257_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8815000.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1308..1931
                     /gene="rplD"
                     /locus_tag="IV257_001896"
     CDS             1308..1931
                     /gene="rplD"
                     /locus_tag="IV257_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8815001.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1931..2221
                     /gene="rplW"
                     /locus_tag="IV257_001897"
     CDS             1931..2221
                     /gene="rplW"
                     /locus_tag="IV257_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8815002.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2260..3093
                     /gene="rplB"
                     /locus_tag="IV257_001898"
     CDS             2260..3093
                     /gene="rplB"
                     /locus_tag="IV257_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8815003.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3136..3414
                     /gene="rpsS"
                     /locus_tag="IV257_001899"
     CDS             3136..3414
                     /gene="rpsS"
                     /locus_tag="IV257_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8815004.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3437..3784
                     /gene="rplV"
                     /locus_tag="IV257_001900"
     CDS             3437..3784
                     /gene="rplV"
                     /locus_tag="IV257_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8815005.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3798..4454
                     /gene="rpsC"
                     /locus_tag="IV257_001901"
     CDS             3798..4454
                     /gene="rpsC"
                     /locus_tag="IV257_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8815006.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4457..4891
                     /gene="rplP"
                     /locus_tag="IV257_001902"
     CDS             4457..4891
                     /gene="rplP"
                     /locus_tag="IV257_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8815007.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4881..5069
                     /gene="rpmC"
                     /locus_tag="IV257_001903"
     CDS             4881..5069
                     /gene="rpmC"
                     /locus_tag="IV257_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8815008.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5094..5360
                     /gene="rpsQ"
                     /locus_tag="IV257_001904"
     CDS             5094..5360
                     /gene="rpsQ"
                     /locus_tag="IV257_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8815009.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5418..5786
                     /gene="rplN"
                     /locus_tag="IV257_001905"
     CDS             5418..5786
                     /gene="rplN"
                     /locus_tag="IV257_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8815010.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5823..6131
                     /gene="rplX"
                     /locus_tag="IV257_001906"
     CDS             5823..6131
                     /gene="rplX"
                     /locus_tag="IV257_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8815011.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6159..6698
                     /gene="rplE"
                     /locus_tag="IV257_001907"
     CDS             6159..6698
                     /gene="rplE"
                     /locus_tag="IV257_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8815012.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6717..6902
                     /locus_tag="IV257_001908"
     CDS             6717..6902
                     /locus_tag="IV257_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8815013.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6938..7336
                     /gene="rpsH"
                     /locus_tag="IV257_001909"
     CDS             6938..7336
                     /gene="rpsH"
                     /locus_tag="IV257_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8815014.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7368..7904
                     /gene="rplF"
                     /locus_tag="IV257_001910"
     CDS             7368..7904
                     /gene="rplF"
                     /locus_tag="IV257_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8815015.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8065..8421
                     /gene="rplR"
                     /locus_tag="IV257_001911"
     CDS             8065..8421
                     /gene="rplR"
                     /locus_tag="IV257_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8815016.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8442..8942
                     /gene="rpsE"
                     /locus_tag="IV257_001912"
     CDS             8442..8942
                     /gene="rpsE"
                     /locus_tag="IV257_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8815017.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8957..9136
                     /gene="rpmD"
                     /locus_tag="IV257_001913"
     CDS             8957..9136
                     /gene="rpmD"
                     /locus_tag="IV257_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8815018.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9180..9620
                     /gene="rplO"
                     /locus_tag="IV257_001914"
     CDS             9180..9620
                     /gene="rplO"
                     /locus_tag="IV257_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8815019.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9620..10915
                     /gene="secY"
                     /locus_tag="IV257_001915"
     CDS             9620..10915
                     /gene="secY"
                     /locus_tag="IV257_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8815020.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10977..11624
                     /locus_tag="IV257_001916"
     CDS             10977..11624
                     /locus_tag="IV257_001916"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8815021.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11815..12033
                     /gene="infA"
                     /locus_tag="IV257_001917"
     CDS             11815..12033
                     /gene="infA"
                     /locus_tag="IV257_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8815022.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12066..12182
                     /gene="rpmJ"
                     /locus_tag="IV257_001918"
     CDS             12066..12182
                     /gene="rpmJ"
                     /locus_tag="IV257_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8815023.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12200..12565
                     /gene="rpsM"
                     /locus_tag="IV257_001919"
     CDS             12200..12565
                     /gene="rpsM"
                     /locus_tag="IV257_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8815024.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12593..12982
                     /gene="rpsK"
                     /locus_tag="IV257_001920"
     CDS             12593..12982
                     /gene="rpsK"
                     /locus_tag="IV257_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8815025.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13062..14000
                     /locus_tag="IV257_001921"
     CDS             13062..14000
                     /locus_tag="IV257_001921"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8815026.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14028..14408
                     /gene="rplQ"
                     /locus_tag="IV257_001922"
     CDS             14028..14408
                     /gene="rplQ"
                     /locus_tag="IV257_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8815027.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14680..15519
                     /locus_tag="IV257_001923"
     CDS             14680..15519
                     /locus_tag="IV257_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8815028.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15495..16364
                     /locus_tag="IV257_001924"
     CDS             15495..16364
                     /locus_tag="IV257_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8815029.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16354..17151
                     /locus_tag="IV257_001925"
     CDS             16354..17151
                     /locus_tag="IV257_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8815030.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17354..18100
                     /gene="truA"
                     /locus_tag="IV257_001926"
     CDS             17354..18100
                     /gene="truA"
                     /locus_tag="IV257_001926"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8815031.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18555..21224)
                     /locus_tag="IV257_001927"
     CDS             complement(18555..21224)
                     /locus_tag="IV257_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8815032.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         7145 a         3903 c         4609 g         5770 t
ORIGIN      
        1 caggatcagg aaaaaagaca ggagtattat aaatagaaga aacacgaaat aaaagagtca
       61 gtattttcta aatgaaactt tatgaaaaca aaatgttttt tacaagaaaa agccttgctt
      121 tgtgagttta tatatattat aatgacaaag gtgcgtttac tttagtgaat tgcacagacg
      181 taatcagaat gatttcgtcg gctaagaaac gagaggttgc gacacgcccg gacgctttgc
      241 cacgaacgag cgtgacggaa attttcgtgg agctatgtct acttttcaaa ataggcgaag
      301 gagggaacaa gatggcaaaa caaaaaattc gtatccgttt aaaagcgtat gaacaccgta
      361 ttttagatca atcagcggat aaaatcgtgg aaactgcaaa aagaactgga gctagcgtgt
      421 ctggtccaat tccattacca actgaacgca gtctttacac aatcatccgt gcgactcata
      481 aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca caaacgtctt atcgacattg
      541 tgaacccaac accaaaaact gttgatgctt tgatgaagct agacttacca tctggtgtaa
      601 acatcgaaat caaattataa aactaaaaca aaaataatgg aggtgtactc atgaccaaag
      661 gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac tgaatctggt gaattaattc
      721 cagtaactgt tgtagaagct actccaaacg tagtactaca agtgaaaaca atggaaacgg
      781 atggctacga agctattcaa gttggttacc aagatatgcg tgaagtttta tcaaacaaac
      841 ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa gcgcttcatt cgtgaattca
      901 aaaatgttga gctaggagaa tatgaagtag gaaaagaaat tacagtagat gttttccaag
      961 caggagacat tattgatgta acaggtacta cgaaaggtaa aggattccaa ggggttatca
     1021 aacgccacgg acaaagccgc ggaccaatgg ctcacggttc tcgttaccat cgtcgtcctg
     1081 ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa caaacgtctt gctggccgta
     1141 tgggtggcaa ccgcgtaact atccaaaact tggaagttgt acgtgtcgat gcagaaagaa
     1201 atgttatctt gatcaaaggt aatgttcctg gagctaaaaa atctttaatt actatcaaat
     1261 cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa ctaaggaatg ccgaatgtag
     1321 cattattcaa acaagatgga agccaaaacg gtgaaatcac acttaacgaa gaaatcttcg
     1381 gaattgaacc taatgaaagt gttgtttacg atgcaatcgt aatgcaacgt gcgtcattaa
     1441 gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg cggcggcgga cgtaaaccat
     1501 ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat ccgctcacca caatggcgtg
     1561 gaggtggcgt agtctttgga ccaacaccac gttcttacag ctacaaactt cctaaaaaag
     1621 ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt tgctgaaaac aacttggtag
     1681 caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga attcaaacaa gttcttgcta
     1741 acttatctat cgactctaaa gtattggttg ttctagaaaa aggtaacgac tttgctgctt
     1801 tatcagcacg taacttacca aacgtttctg tagtaacttc tgataatgtg agtgtgctag
     1861 acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc tcttactcaa attgaggagg
     1921 tgcttgcata atgaacttac tagacgtaat caaacgccca gtgatcactg aaaaatcaat
     1981 gcttgccatg gatgacaaga aatacacttt tgaagtggac actcgcgcaa acaaaacttt
     2041 agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc aaaaacgtga acatcgtgaa
     2101 cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga tatacaaaaa aacgtcgcaa
     2161 agcgatcgtt acattgactg aagattcaaa agaaatccaa ttattcgaag ctgctgaata
     2221 agcactaata gataactcat taaattagga gggaaaaacg tggcgattaa aaagtacaaa
     2281 cctacctcaa atggtcgtcg taacatgaca ggttctgatt ttgctgaaat cacaacatca
     2341 acacctgaaa aatcattgtt acaaccattg aaaaaccatg ctggacgtaa caacaacggc
     2401 cgcatcactg tacgtcatca aggtggcgga cacaaacgcc aatatcgcgt gatcgacttc
     2461 aaacgtaaca aagataacgt tgttgcaact gttcaaacga tcgaatatga tccaaaccgt
     2521 tcagctaaca tcgcgttagt acactatgaa gatggagtga aagcttacat cttagcacct
     2581 aaaggactag aagtgggtat gaaacttgtt tcaggtccag aagcagatat caaagtcgga
     2641 aatgctcttc cattagaaaa cattccagtt ggtactgtta tccacaacat tgaaatgaaa
     2701 cctggtaaag gtggacaatt gatccgttct gctggtacaa gtgctcaagt acttggtaaa
     2761 gaaggcaaat acgtattgat ccgcttgaac tctggtgaag ttcgtatgat cttagctgca
     2821 tgccgtgcta caatcggttc tgtaggtaac gaacaacacg aattaatcaa catcggtaaa
     2881 gctggacgct ctcgttggat gcgtaaacgc ccaactgtac gtggtagcgt aatgaaccct
     2941 aatgatcacc cacacggtgg tggtgaaggt aaagctccta tcggacgtaa agcaccagtt
     3001 tctccttggg gtcaaccagc tcttggttac aaaactcgta acaaaaaagc taaatcagac
     3061 aaacttattg ttcgtcgtcg caaaaataaa taattacttt gaccatgtgt cgcacatttt
     3121 gagaggaggt tcaccatggg tcgtagttta aagaaaggac cttttgtcga tgagcattta
     3181 atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa aagttatcaa aacttggtca
     3241 cgccgttcaa cagttttccc aagttttgtt ggatatacca tcgcagttta tgatggacgt
     3301 aaacatgtac cagtttacat ccaagaagac atggtaggac acaaattagg tgaatttgca
     3361 cctacaagaa cttatcgtgg acatgctgct gacgataaga aaacgaaacg ctaatttgag
     3421 gggaggataa atccaaatgg cagaacaaat cacatcagct aaagcaactg caaaaacagt
     3481 tcgcacttca cctcgaaaag cccgtttagt aattgacctt atcagaggta aaagtgtcgc
     3541 agatgcaatt tcaatcttga aattcacacc aaacaaatct gctggaatca ttgaaaaagt
     3601 attgatgtca gcagttgcga atgcagaaaa taactttgac ttagatgttg aaaacttggt
     3661 agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa cgtttccgtc ctcgtgcaaa
     3721 aggatcagct tctccaatca acaaacgtac aagtcatatc acggtagtcg tatcagaaaa
     3781 ataaggaggg ataatcagtg ggtcaaaaag tacatccaat tggaatgcgt gtaggcatca
     3841 tccgcgactg ggatgctaaa tggtatgctg aaaaagagta tgctgagttc ttacacgaag
     3901 atttaagaat ccgtaaattt atttcaacta aacttgctga tgctgctgta tcaacagttg
     3961 aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca cacagctaaa ccaggtatgg
     4021 ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa agaattaaac aaattaactg
     4081 gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc agatctagat gcaaaattag
     4141 taggtgaagg aatcgcacgt caattagaaa accgtgttgc tttccgtcgc gctcaaaaac
     4201 aagccatcca acgctcaatg cgtgctggcg ctcaagggat caaaactcaa gtatctggtc
     4261 gtctaaacgg agcagatatc gctcgttcag aaggatactc agaaggaact gttcctcttc
     4321 atactttacg tgcggacatt gattacgcat gggaagaagc agatacaaca tacggaaaac
     4381 taggagttaa agtgtggatc tatcgtggag aaattcttcc aactaaaaaa aacactgaga
     4441 aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa caccgtcgtg aattccgcgg
     4501 aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca ttcggtgaat acggtttgca
     4561 agctgttgat tcacattgga tcacaaaccg ccaaatcgaa gctgctcgta tcgcaatgac
     4621 tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt ttccctcaca aatcttatac
     4681 tgccaaagca attggggtac gtatgggttc tggtaaaggg gcacctgaag gatgggttgc
     4741 accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc gttcctgaag aagtagctcg
     4801 tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa actaagatcg taaaacgtga
     4861 ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag agaattaacc actgccgaaa
     4921 tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa tcttagattc caattagcaa
     4981 caggtcaatt agaaaacact gcacgtatta aagaagtacg taaatcgatt gcacgcatca
     5041 aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga ggccattaag cgtatgactg
     5101 aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt ttcagacaaa atggataaaa
     5161 caattaccgt tgctgtcgaa acaaagaaaa accaccctat ctatggcaaa cgcatgaact
     5221 attctaagaa atacaaagtg catgatgaaa acaacacagc aaaagttggc gacatcgtga
     5281 gaattatgga aactcgtcca ttatcagcta caaaacgttt ccgtttgttg gaagtagtcg
     5341 aagaagcagt tattatctaa taacacgaga ttttcctata tagattgaat gcaaaatctg
     5401 aaaggaggat acacaacgtg atccaagcag aaagtcgttt aaaagtcgct gataactcag
     5461 gcgcacgtga aattttgacg atcaaagtcc ttggtggatc tggtcgtaaa actgctaaca
     5521 tcggtgatgt gatcgttgct acggttaaac aagcaacgcc aggtggggtt gtcaaaaaag
     5581 gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg agctcgtcgt acagacggtt
     5641 catatattaa atttgatgaa aatgctgcag tgattattcg tgatgataaa agccctcgtg
     5701 gaacacgtat ctttgggcca gttgcacgtg aactacgtga aaacaacttc atgaagatcg
     5761 tttcactagc accagaagtt ttataatctt gagacactta tcaaaggagg tgcgaaacag
     5821 ttatgtttgt taaaaaaggc gataaagtta aagttatcac tggaaaagac aaaaacaaag
     5881 aaggcgttgt actagcagcg ttcccgaaaa aagataaagt tattgtcgaa ggtgtaaatg
     5941 tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca aggtggaatt gttgagatgg
     6001 aagcgccaat tcatgtttct aacgtgatgg tggttgactc tactggtgta gctggccgag
     6061 ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc taaaaaaacc ggtgaagttt
     6121 tagataaata aaagtttaag gaaggagggt cttactgaat gaaccgccta aaagaaaaat
     6181 atcttaaaga agtaactcca tcattgatgg aaaaatttaa ctatagctct gttatgcaaa
     6241 cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg tgatgcggta tcaaacgcta
     6301 aaaacttaga taaagcagtt gaagaattaa ctttgatcac tggtcaaaaa ccaatgatta
     6361 caaaagctaa aaaatcaatc gctggattcc gtttacgtga aggaatgcca attggtgcaa
     6421 aagttacatt gcgcggagaa agaatgtacg aattcttaga caaattggtt tctgtttcac
     6481 taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc ctttgacggt cgtggaaact
     6541 atactttagg tattaaagaa caattgatct tcccagaagt agattatgat ttagtagata
     6601 aagtacgtgg tatggacatc gttattgtaa caacagcaaa ctctgatgaa gaatctcgtg
     6661 agttgttgac acaattaggc atgccattcc aaaaataatt aaaggaggcg aactacgtgg
     6721 ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa attttcaaca caagcttata
     6781 ctcgttgcga acgttgcgga cgtccacatt cagtttatcg taagttccat ctttgccgta
     6841 tttgcttccg cgaacttgcc tataaaggtc aaattcccgg cgtgaagaaa gctagctggt
     6901 aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg gtcatgacag atccaattgc
     6961 agattttcta actcgtatcc gtaacgcaaa catggttaaa catgagtctt tagaagtacc
     7021 tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa cgtgaaggtt tcgttcgtga
     7081 tgttgaatac atcgaagatg acaaacaagg cgtgatccgt gttttcctaa aatttggtaa
     7141 aaacggcgaa cgtgttatca caaacttaaa acgtatctct aaaccaggtt tgcgtgctta
     7201 tgtcaaagct gacgaagtac caaaagtatt gaacggttta ggtatcgcaa tcatttcgac
     7261 ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa aatatcggcg gcgaagttat
     7321 cgcttacgta tggtaatcaa gtagaatcta caaggaggtg tctttaagtg agccgtatcg
     7381 gaaataaagt cgttgttatc cctgaaggag taacagttac tcaagacgga aacaacgtta
     7441 cagttaaagg accaaaaggt gaattgactc gtactttttc agcagatatc aaaatgaata
     7501 tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag taaagaaatg aaaacaattc
     7561 atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg tgtcagcgaa ggtttccaaa
     7621 aagcgttaga actaatcggg gttgggtacc gtgcacaatt acaaggatca aaacttgtat
     7681 tgaacgttgg ttactcacat ccagtagaaa tcacaccacc agctggagta actgttgaag
     7741 taccagctaa cacacaagta attgttaaag gtgcgaacaa agaagaagtt ggcgaattag
     7801 ctgcaaacat tcgtggaact cgtcctccag aaccttataa aggtaaaggt attcgttatg
     7861 ttggcgaata tgtacgccgt aaagaaggta aaactggtaa ataatcgttt agaaaaattg
     7921 catagaagcc agcagcagga aaaaggcatt tggctgaagt ttaccttcaa aaaaatacat
     7981 ttttacttgt gccgcagctt ctatttttcg aaacgtattt actgcttgaa atagtagtac
     8041 aataaaaata aagaggtgac aattgtgatt acaaaaccag ataagaacaa aacacgtcaa
     8101 aagagacatc gtcgtgtacg taacaatatc tctggtactg ctgagcgccc acgcttgaac
     8161 gtttttcgtt ctaacaaaaa catctacgct caaattattg atgacgtagc gggtgtaacg
     8221 ctagcaagtg cctctacctt ggataaagaa atttcaggtg gaacaaaaac agaagcagca
     8281 caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa aaggcattaa agtagtagtc
     8341 tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag ctttagctga agctgctcgc
     8401 gaaaatggac tagaatttta gaagaaggag gaataccatt catggtttat atcgatccaa
     8461 aacatttgga attagaagac cgcgttgttg caattaaccg tgtaacaaaa gttgttaaag
     8521 gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg tgacaaaaac ggacacgtag
     8581 gatttggtac aggtaaagct caagaagttc ctgaagcaat ccgtaaagca attgaagatg
     8641 cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac aatcccacac gaagtaatcg
     8701 gtgcttttag tggtggtaga atcctaatga aacctgctgt agaaggttct ggggttgctg
     8761 ctggtggacc agttcgtgcc gtattagaat tagctggggt agcagatatc acttctaaat
     8821 ctttaggctc aaatacacca atcaacgttg ttcgcgcaac agttgaaggt ttgaaacaat
     8881 tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc agttgaagaa ttaatcggat
     8941 aaggaggaca acaataatgg ctgaattaaa agttacttta aaacgcagta ttatcggacg
     9001 tcctcaaaac caacgcgata cagttaaagc gttaggtctt acaaagatta acagttcagt
     9061 ggttaagcct tctaatgaag caattaaagg catgatcaat actgtttctc acctagtgga
     9121 cgttgaagaa atttaattta gaaaaatcat aactagaaca ttaaggaggt gccaaaccta
     9181 tgaaacttca tgaattaaaa cctgctgaag gttcacgcca agtacgtaac cgtgttggtc
     9241 gtggtacttc atctggtaac ggaaaaactg ccggacgcgg acaaaaagga caaaaagctc
     9301 gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca aacaccattg ttccgtcgtt
     9361 taccaaaacg aggattcaca aacgtgaacc gtaaagacta tgcagtcatt aatcttgatg
     9421 tattgaaccg ctttgaagac ggagctgaag taactcctgt tgctttagtt gaagctggaa
     9481 tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa tggggaacta aacaagaaat
     9541 taactgtgaa agcagcaaaa ttctctaaag cagctcaaga agcaattgaa gcggctggtg
     9601 gatcaatcga ggtgatctaa tgtttagact attaaaagat gcttttaaag taaaagacat
     9661 tagatcgaaa atcctattta ctgtatttgt gttgtttgta tttcgcctag gtgcacatat
     9721 tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt tcttctttgc catttttcaa
     9781 catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt tccattttct caatgggggt
     9841 ttcaccatac attactgctt ctatcgtcat tcagcttctt caaatggaca tcgtgcctcg
     9901 atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa aaactgaatc aagcgactcg
     9961 atatctgaca ttagtattgg catttatcca atctgtcggt atcactgctg gattcaatca
    10021 atacgctcaa ttaggtttcg tgaataatcc taacgtagca acttacgtaa cgatcggttt
    10081 gattctaaca gccggtacga tgtttgttac ttggctagga gaacagatca cagaaaaagg
    10141 tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc atttctagat tgcctgaaag
    10201 tatcagagaa atttatgaag attactttat aaatattgat ccttcagaca tatggaaatc
    10261 tgctattttt gtggccattt tactagtagc ggtcttgttg atcgtgacat tcgtaacatt
    10321 tttccaacaa gctgaacgca agatcccgat ccaatatacg aaacgcgtag ctggcgcgcc
    10381 tacaagcagc tatctgccgt tgaaagtaaa tgcagctgga gttattccag ttatctttgc
    10441 tagttcgttt attgctacgc caaatgctgt tttacaagca ttgagcggta gattcggttc
    10501 tgaaaactgg tacaaagtga tgatggagat ctttaactat aatacagcac ctggtgctac
    10561 gatttatacg attctgatcg ttgccttcac tttcttctac gcctttgttc aagtaaaccc
    10621 tgagaaatta gcggaaaact tgcaaaagca aggaagttat ataccaagcg tacgccctgg
    10681 taaggggact gaagagtatg tctcaagatt actgatgcgt ttaagtacag ttggtgcagt
    10741 cttccttggt ttagtggcat tgcttccaat cattgctcaa atggtttggg atcttccaca
    10801 atcaatcgga ttaggcggta caagcttact gatcgttatc ggtgtagctt tagaaactgc
    10861 gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt ggattcattg agtaagaaag
    10921 cgtgttgagg tttcccctca acacacgttc tcacacattt attaggaggg aaaacaatga
    10981 acctcatttt aatggggctg ccaggcgcag gtaaaggaac acaagctgaa aaaatcattg
    11041 atgcttatgg tattccgcac atctcaacag gagatatgtt ccgcgcagcg atgcaaaacg
    11101 aaacagctct tggcctggaa gcaaagtctt atatggataa aggtgcatta gttcctgatg
    11161 aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc agatacagag aaaggcttct
    11221 tattagatgg ctttccacgt acgctagatc aagcaaaagc tctggacgca atgctaaaag
    11281 atttgaataa aaaaattgat gctgtcatcg atatccatgt tggtgaagaa atcttggtcg
    11341 aacgtctagc cggccgattt atctgcagca attgtggagc aacttaccat aaaattttca
    11401 accctacaaa agttgaagac acatgtgatc gttgcggtgg gcatgaattc tatcaaagag
    11461 aagatgataa acctgagacg gttaaaaatc gtctggctat caacatcaaa aatagtgaac
    11521 caatcttggc ttattataaa gaacaaggtt tgctaaacac tattgatggt gctcgtgaaa
    11581 tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa atagcacgat tgcccgtagc
    11641 cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa ttatgataga atatgtcagt
    11701 ccgacttcta gagaatcctc tagatgatcc attttgagaa ttttgaggtg ggaactgcag
    11761 tttccgccat tttatcgtgt gaaaatgcga aacaagtaca aggaggtact gtgcgtggca
    11821 aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa ctttgccgaa tgcaatgttt
    11881 aaagttgaac tagaaaacgg acaccaagtt cttgctactg tttcaggtaa aattcgtatg
    11941 cattacatcc gtatcttacc tggtgacaaa gtaacggttg aattgtctcc atacgatctt
    12001 aaccgtggcc gtattactta tcgctttaaa taattgtact ccgtaaaact acaggaggta
    12061 ttatcatgaa agtaagacca tcagtaaaac caatgtgcga acattgtaaa gtaattcgtc
    12121 gtaaaggacg cgttatggtg atttgcccag caaatccaaa acataaacaa cgtcaaggat
    12181 aattggaggt gctataaata tggctcgtat tgcaggagta gatattcctc gtgataaacg
    12241 tgtagtagtt tcacttactt atatctatgg tattggtaac actactgcta aaaagatctt
    12301 agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa ttaacgaatg aacaaacaga
    12361 tgctatccgt gcagaagtag ataaattaaa agttgaaggg gatcttcgtc gtgaagtgaa
    12421 cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt ggaatccgtc atcgccgtgg
    12481 attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt actcgtaaag gcccagctcg
    12541 cgctatcgct ggcaagaaaa aataattctt aagtgaagga ggttaaaact tcatggcagc
    12601 aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata gaagcaggta ttgcacatat
    12661 ccattctaca ttcaataaca ctatcatcat gatcactgat actcacggaa atgcattagc
    12721 gtggtcatca gctggttcat taggtttcaa aggaagcaaa aaatcaactc cttttgccgc
    12781 tcaaatggca gcagaaactg ctacaaaagc agcaatggaa cacggattaa agactgttga
    12841 agtaacagtt aaaggacctg gttctggacg tgaagcagca attcgttcat tacaagctac
    12901 aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt cctcataatg gtgcccgccc
    12961 tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg agttttatca gtaacgtcat
    13021 tgaacaagac acttttcgtt ttgaaagggg taaagataag aatgattgag ttcgaaaaac
    13081 caagaatcgc aaaaattgat gaagaaaaag attatggcaa gttcatcgtt gaacctcttg
    13141 aaagagggta tgggactact ttagggaatt ccctacgtcg tattttatta tcttctttgc
    13201 caggtgctgc catcactaat attcaaatcg atggtgtact gcatgaattc tctaccatca
    13261 aaggtgttag agaagacgtc actcaaatca tcttgaacat taaaggctta gcgctgaaaa
    13321 tgtatggcca agaagaaaaa acccttgaaa tcgatattac cggacctgct acagtaactg
    13381 ctggcgatat tatcgttgat agtgatgtag aaattcttaa caaagatatg tacatctgta
    13441 gtgtctctga aggagctacg ttccatgctc gcttaacagt gaaacctgga cgtggttatg
    13501 ttcaagcaga tgaaaataaa aaagaagata tgccaattgg cgttcttcca gtcgattcaa
    13561 tctacacacc tgttcgtcgt gtgaactatc aagttgagaa cacacgtgtt ggacaccgtg
    13621 aagattttga taaattgacg atggagattt ggacagatgg ttcaattgaa ccgttggaag
    13681 caatgagttt gtctgcgaaa atcatgactg aacatttgga tatttttgtg aacttaactg
    13741 atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga aacacaaaaa gaaaaaatgt
    13801 tggaaatgac gatcgaagag ttagacttgt ctgttcgctc ttacaattgt ttgaaacgtg
    13861 caggaatcaa tactgtacaa gaacttacaa ataaatctga gcctgaaatg atcaaagtcc
    13921 gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa attgcacgac ttaggtttag
    13981 gtttacgtaa agaagattaa aaccttttac gaaggaggga aaccaacgtg agttatcgta
    14041 aactaggacg cacatctagc caacgtaaag cgatgttgcg tgatttaaca actgatttat
    14101 taatcaacga acgtattgtg actactgaag ctcgtgcgaa agaagttcgc tcaactgcag
    14161 aaaaaatgat cactttaggt aaacgcggag atttgcatgc acgtcgtcaa gctgcggctt
    14221 tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga ggacatcgtc attgaatcag
    14281 ctttacaaaa actgttcaac gatattgcac ctcgttacgc agaacgtcaa ggcggataca
    14341 cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc accaatggtt gtcttagaac
    14401 ttgtctaaaa tcacactaat aacatcactt tatagatgat tcttttagag tgttatgatg
    14461 ttggaagata tttttccgag tctagctcag cgctgctcct caagcgaata tcgcttgggg
    14521 agtaagcact catcataaag tgttttttta taccttcaat tacctgtcat gacagatatg
    14581 tttgaaggca taaaaaaaca ttttaagcga gaaataattg aaaattttgt ctttttttga
    14641 tagtgttagt aggaacttta ggatgaagaa ggagtaatca tggagccaat tattgaatta
    14701 gaaaagataa attataaata ccagccagat gatcttcgcc ccgcattgaa agatgtctcc
    14761 tttacaattg ataaaggtga gtggatcgct attattggac acaatggttc aggaaaatcg
    14821 acacttgcca aaacgatcaa tggattgctt ctgccagaat cagggatcgt gaaagtggga
    14881 aatcaaatat tggatgaaga aaatatttgg acgatccgac agatggttgg aatggttttt
    14941 caaaatccag acaatcagtt tgttggttcg acagtagaag atgacgttgc ttttggtctt
    15001 gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg tgaaagatgc acttgagaaa
    15061 gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt tatcaggagg gcaaaaacaa
    15121 cgtgttgcca ttgccggcgt ggttgcctta cgacctgata tcattatttt agatgaagca
    15181 accagtatgc tagatccaga aggtcgtgaa gaagtgatct ctacgatcaa gaagatcaaa
    15241 gaagaaagtc aattgaccgt tatatctatc actcatgata ttgatgaagc agctaatgcc
    15301 aatcggatac tagtgatgag acaaggcgaa cttgtccgtg aaggtacacc aaaagaaatt
    15361 ttttctgctg gtcctgaact gatcgactta ggtctagatc taccatttcc tgaaaaactg
    15421 aagagcgcac tgaaagaacg tggggttgat gtgcccagtg aatatatgac agaagaaagg
    15481 atggtggatt ggttatggac atccgttttg aacaagtaga cttcacttat cagccgaata
    15541 caccatttga acaaagagcc ttatttgaca tcaatatgac gatcaaagaa aacagttata
    15601 ctgcattagt agggcatacc ggaagcggga aatcaacctt acttcagcat ttgaatgcgc
    15661 ttgttaagcc aacaagcggt acagtccata ttggagaacg agatattcag ccggatacgg
    15721 ataataagaa tttaaagccc atccgaaaaa aagttggcat cgtctttcag tttccagaag
    15781 cacagctatt cgaagaaacg gtagcaaaag acattgcttt cggtcctaaa aactttggtg
    15841 tcagcgaaga agaagcacta gtcctagcaa aagaaacatt agaacaagtt gggctggatg
    15901 aaagctattt ggaacgttcg ccatttgaac tttcaggagg gcaaatgcgt agagtagcaa
    15961 tcgctggtgt gcttgccatg aggccggaag tacttgtatt ggatgaacca acggcaggac
    16021 ttgatcccca gggcagaaaa gaaatgatgg agatgttttg gcgtcttcat aaggaacagc
    16081 agatcactat tgtattagtt acgcatttaa tggatgatgt agccaatttt gcagattatg
    16141 tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga gccgcaagag gtctttcaaa
    16201 acattgaatg gctaaaagaa aaacaactag gcgtacctac tgccaccgag tttgcagaag
    16261 aattaatggc taaagggatg aactttgcta ccttgccttt gacagctgaa gaattagcag
    16321 acgctatcgt ccattatgct ggaggaaatg cccatgatga ataaattgat atttggtcgt
    16381 tatatgcctg gagattcctt gatccatcga ttggatcccc gagctaaatt attagccagt
    16441 ttttatttta tcggaatcat ttttttagca aataattggg aaacgtacct tttgttggca
    16501 tttttcacat tactttctgt cttcttgtca aaagtagatt tgaaattttt tatccgaggc
    16561 attcgcccat tgatctggct aatcctcttc actgtagctt tgcaggtctt gtttacaagt
    16621 ggcggagaaa cttattggag ttggggtgtt ttccatatca ctgaatttgg tgtgatgaac
    16681 ggcctcttta ttttctgtcg attcgtgttg atcattttta tgtcgactct actcacgctt
    16741 actacaccgc ctttggaatt atcggatgcg attgagtact tgcttcgtcc attgaaagcc
    16801 gtacggttcc cagtccacga aatttcattg atgctgtcga ttgctcttcg ttttgtgcct
    16861 acattgatgg acgaaacaga aaagatcatg aacgctcagc gcgctcgcgg tgttgatttt
    16921 ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc ctttgctgat tcctttattt
    16981 gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta tggaagcaag aggctacaaa
    17041 ggtggggaag gaagaacgaa atatcgtgta ttgcattggc ataatagaga cacgatggtt
    17101 atcattgctt ttgtattatt aactgtgcta ctgatctttt taagaggata aacgttcagt
    17161 gggccgctat ctattctttt cttgctgatc tgtttgcaag aaaggatgca agcagtgtat
    17221 gacaagaaat attagggtct gtggcagact aaggtcacag gcctctttct tttatctgaa
    17281 aagaaagaac gaaaaaattt tatagaaaaa tcattattcc agataacctt ttaggaacaa
    17341 aggagagaaa aacatggtac gttacaaagc aatcattgcg tacgacggaa caaattttaa
    17401 tggattccaa aaacagccaa atgggcgtac tgttcaagaa gaagtagaaa aaacattaca
    17461 aaaaatggca aatggaaaag aaatcacggt ctttggttcg ggaaggacag atgccggagt
    17521 acatgcgatc gggcaagtaa tccattttga ttatccagag gagcgtccgt tagaacgaat
    17581 gcgtttcgcg ctagataccc agtcaccaga ggatattgct gtaagacaag tagaaattgt
    17641 ttcagaagac ttccacgcaa gatatttggt caaagaaaag acgtatcaat ttcgagtgga
    17701 tatagggaaa ccacgcagtc cttttcgacg ccattatgca agttattttc cttacccact
    17761 tgatttggaa aaaattcagc gggcgcttcc tgatttatta gggactcacg atttcacctc
    17821 tttttgtgca tcaggaagct cgatagagga taaaatacgt acgatctacg aggctaagat
    17881 ggaggtaaat gaaacaaaag acgaattgtt gtttacattt aggggaaatg gcttcttgta
    17941 taagatgata cggatcttag taggaacgct tttgaaaatt ggcaatggcc gattgcctga
    18001 agatagtatc ccagatatta ttgcgaaaaa agatcgtaat gcagcaggac ctacagcaca
    18061 ccctgaagga ctttatttgt atgaagtgaa atatgaataa gaggttatga tagaagtggt
    18121 cagcttcaag aaataagaag gtatttacga aaattacttc tcaaaatttt gcaaaatttc
    18181 ggcttatttt cgaagaagct acttctggaa caccgtttaa ggaagaggtc gtgaaatcag
    18241 cgttttgacc tgagtattag agaaacaaac gggaaaatag ctcctcatat tttgccgttt
    18301 gttgagctaa taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct
    18361 gtcatttaac tctgagtcac aaagagcaat ttcaaaaaac agcttctcat ctttgatgaa
    18421 tatcgtgact tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc
    18481 tgtaaccaaa aaacgatgga acgttgtttt gattgccctt ggtgcggaca gtctgaacag
    18541 gttccatcgt ttctttattt tctacgtctt cttctgtgtg tgtttcgtgg ttgttcttct
    18601 attgctggat caatcgtgcc ataaaagggg cgattgcggt caaggtacca gacataaaga
    18661 ataaacaatg caatacatag gacaaaaagg attgcaccta tcgtaaatcc ttcaggcaag
    18721 taactgaaag tgacagtatg tttacctgct ggaactttta tcatcaagaa tgcgtctttg
    18781 aaattgctga ccttgactgg tttcccatca attttagctt tccatccttt gtcataagga
    18841 atagttgtca ccaacatgga atcttcagcg gtatttacag tcccagaagc ttttctgcca
    18901 gtggcttcca tttcgacacc tttttctttg atctgattca ttgcggcttg ataggctaca
    18961 acatctagac caacgacttt aggctccatg aaacttattt ctttcgtccc ataaaaactt
    19021 gcagtaaatg tcactgttgt ctcttgagga taatagccaa gatcatagta ttgaccagaa
    19081 atgttgattt gcgtttttcg cgatgtacca ttgacagtga tcgtcgcact agaactttct
    19141 aattgcgcaa aattggttgg gaacaaactt aagtaagctt gtgtatttgc aggcacatgg
    19201 acttcccaag tgagctcttt tggctggtta tctgctgttt ctgagtaagt gacgccagtc
    19261 gttgtctttg tgatcgtcgt attcaactga ttcttcaaag taggtgtata gaatgtaaaa
    19321 tactgttcat ttagaccaga caatgtattg atcaagtttg tctgactggt caaattatct
    19381 gtcactggct gctcaacatc attaacggac aacggtgcag taaatgctag tggcaatgca
    19441 ttgacattct gatacagtgt atagtcgccg ctttgaccag cttggcggaa gccatattta
    19501 ctgaacgtgc tttcgttttt agaaatgtta tatttgattc ctgtaaaccc atccatcagc
    19561 aaagtattat tgttataacg gatattcaaa tttgtccctc ttgaacggaa acctaattta
    19621 tccaggtaag aggaagaatt acgatttctt atagaagaaa acaaactgat accactgtaa
    19681 ccatagttga tcgaatcgtt tgcagatact ggatccagat tttccaagcg gtaaaacgag
    19741 tcggaatctt tttttgtttc atttacaaga gctttcaagt caggataagg atcagtatat
    19801 aaactgcggg aagcataatt ccaatcatca agaatgccat ggatcatact gttcgtattg
    19861 accagtgctt cagcagacat gatcaataat aacaatatac tcagatgatt gaatgaaagt
    19921 tttttatgct gataaaatcc cacagcaaaa gcataaatcc ctaagaaaac cactgtcaat
    19981 acaaaggaag tcatcgtcac ataggtgtag cttccttcag gtttgatgcc ccatgccacc
    20041 gcaaagagag cagctacgac caaaatgatt cctaacaaca gcccacgatc gtcttgttct
    20101 atcttttccc atccttttcc tgcaagcagg agcactaaga aagagaacag ataggcataa
    20161 cggaataaaa acatattcgg tgcatgcata ccatgccaga acaaattcaa tggaacaaga
    20221 taaaaacttg cgataagtat ggcgaaaaga ctgccgtaca aaaatttatt tttcaacggt
    20281 actttccgac tggcaaaata aaacagacag aggactaatg gtagcagacc tgcgtaaacg
    20341 aacggaatcg atccgtattt tgtcgtgtca taaacaccaa tcatattttt catgattaga
    20401 tccagataag aagtcgcttc tgttttcaac gtagtgatct gactcagtgc ctcgccattt
    20461 gaccgaagat ccagcactgc tggcaataca atgatcattg aagcgccgcc tgccagtaaa
    20521 gaagtgatac cataaggaac aatcgttttt ttatattctg accagtttgt caatagacgg
    20581 gcgataaaat ataagaaaga aaatacgcca atcataaaac ccatataaaa actggataaa
    20641 aacaagagta aataactgat aaataaaagt ttcggccgtt taagatccat aacacgatga
    20701 atccccaaaa tcactaatgg aagataaaaa aatgcatcca gccacatgat gatctccgaa
    20761 tgtgcagtga taaatgagat caatgcgtaa ctgacgctaa gtgcaacatg cccccagcga
    20821 ggaatcttga acgtacgtgc aagaaaccaa aaagccaatc cggcacagcc gaccttaagc
    20881 aaagtcagaa catacagtgc atctggcatc atctggtttg gaaaaaagaa caccagtggc
    20941 gtgaagatcc cgcctagata gtaagaaatt aaagataaat aattcaatcc tagagaagca
    21001 ttccatgtat aaaaaatact ttgctttcca agaagaacat ttcggaagct ggcatgaaaa
    21061 ttagaaaact gtgaaaaggc atcacttgcc aaaatacttc tgctgcttcc aggatagatt
    21121 ccgatactta gataaacaag tgctaaaatc aaaaacggaa tcaggaaact agctgctgcg
    21181 tagcgtccgt tttctctaaa gaatgacttt aaattttttt tcatatttat tttccaccaa
    21241 atttctatga agttgtattc agtttatcat atccttgttt ttctctgctt aaaaagagtc
    21301 tagttttcac aaaaaaataa aatttagaat aaataacttc ataatttgtt cgtataacaa
    21361 aaataaacgc aaaaaaaggg taggaatcaa aagtcccacc cttaaaaatt tttattaaat
    21421 aaaaaac
//
LOCUS       DACDIP010000047        20129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDIP010000047 DACDIP010000000
VERSION     DACDIP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.047"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1233
                     /locus_tag="IV257_001928"
     CDS             <1..1233
                     /locus_tag="IV257_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8815033.1"
                     /translation="TTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSG
                     ASIAGILGNWKQESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTAL
                     VDWSKKNNGGSWWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEIS
                     ADSESAVIAVRGENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASG
                     TVEDACATTDDANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPD
                     WSTSPFNGIYQGQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGY
                     GFSASDGWLWAVGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGS
                     DNVHFFSGVGKAKFEIEK"
     gene            1256..1783
                     /locus_tag="IV257_001929"
     CDS             1256..1783
                     /locus_tag="IV257_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815034.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            1770..2333
                     /locus_tag="IV257_001930"
     CDS             1770..2333
                     /locus_tag="IV257_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815035.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            2755..3492
                     /locus_tag="IV257_001931"
     CDS             2755..3492
                     /locus_tag="IV257_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815036.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(3509..3925)
                     /locus_tag="IV257_001932"
     CDS             complement(3509..3925)
                     /locus_tag="IV257_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815037.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            4318..5565
                     /locus_tag="IV257_001933"
     CDS             4318..5565
                     /locus_tag="IV257_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815038.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            5833..8223
                     /locus_tag="IV257_001934"
     CDS             5833..8223
                     /locus_tag="IV257_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP8815039.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            8272..10107
                     /locus_tag="IV257_001935"
     CDS             8272..10107
                     /locus_tag="IV257_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8815040.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            10182..11039
                     /locus_tag="IV257_001936"
     CDS             10182..11039
                     /locus_tag="IV257_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8815041.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            11029..12108
                     /locus_tag="IV257_001937"
     CDS             11029..12108
                     /locus_tag="IV257_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8815042.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSEYVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLNVLILLIKEFRKA"
     gene            12447..13247
                     /locus_tag="IV257_001938"
     CDS             12447..13247
                     /locus_tag="IV257_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815043.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            13335..14141
                     /locus_tag="IV257_001939"
     CDS             13335..14141
                     /locus_tag="IV257_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815044.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTAENWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            14372..15241
                     /locus_tag="IV257_001940"
     CDS             14372..15241
                     /locus_tag="IV257_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8815045.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGNVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            15261..15587
                     /locus_tag="IV257_001941"
     CDS             15261..15587
                     /locus_tag="IV257_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815046.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDD"
     gene            15621..16883
                     /locus_tag="IV257_001942"
     CDS             15621..16883
                     /locus_tag="IV257_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815047.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVANFSKSVYLVGRALVVSAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            16963..18339
                     /locus_tag="IV257_001943"
     CDS             16963..18339
                     /locus_tag="IV257_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8815048.1"
                     /translation="MAMKLLEKVKNRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISDTPHMLVAGTTGSGKSVLLNEILVTAICHAMPDELKLG
                     IVDPKRVEFGRYKKLPYMLADPITDMDEAYEFFEYLVILMNERYKLMEKAGVQNITQY
                     NKYAEKHGIEKFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMFIKLNGGNKIRAQG
                     AYISNEEIEAIFDHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     VDPEPQTSNNTNNEQPISSKTKPKTTDPKVSVKVTITTDNSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            18336..18965
                     /locus_tag="IV257_001944"
     CDS             18336..18965
                     /locus_tag="IV257_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815049.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNERKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            18977..19435
                     /locus_tag="IV257_001945"
     CDS             18977..19435
                     /locus_tag="IV257_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815050.1"
                     /translation="MLTILKLYREGFNEKVSIGESIEYRGDKYIIIRILKINKHYFSK
                     SISLTVRVLVQKVGDLSDYYNCKREVIVDNRYDQSKDDKRIIKIGDIVFPEKGIAYEV
                     VSINSFRYEFVDLVVEYSVRLIVPWSKEEINEALKDERKSTFKVLEGEKK"
     gene            19432..19911
                     /locus_tag="IV257_001946"
     CDS             19432..19911
                     /locus_tag="IV257_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815051.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGRERVDYITYS
                     VDNTVRCYEIKVTLSDLKSKAKQTFIGDYNYLVVTSDLWEKMLQDDTLMRKYYKQGVY
                     VFNEKYPSMISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIVEIPE"
BASE COUNT         7593 a         2864 c         4104 g         5568 t
ORIGIN      
        1 actactgaag gtagtgggtc aggaacagat agcgatggaa acgatactgg aagtggcgct
       61 gtaacaggtg atgtagccaa aaatattgaa gatatttaca aatacatgaa tggagaatat
      121 ggcttatcag gtgctagtat tgcaggtatt ttaggtaact ggaaacaaga atcaggtatt
      181 gatcctcgag cagtagaagg gtattacgga gatggacaga gagaagttga cttcatcaaa
      241 caatttactg atggtctgat gggtattggc tatggtcaat ggacagctgg gcgacataca
      301 gcattagttg attggtctaa gaaaaataat ggcggttctt ggtggactac agaagcccaa
      361 ttggacttta tgttcaaagg tgatggggga ttcgtaggaa tattaaaaga ttatgcttta
      421 aatgcttctg atgatgtcgt agaaaatgca gtaacttttc ataaaaactg ggaaatttca
      481 gctgattccg aatcagctgt aatagctgta cgtggtgaaa atgcaaaaga aatctggaag
      541 tacatgaaaa gcaagggtat ggacggtgca aaagatgaaa gtaagatcaa taaaatatcc
      601 ggaggttcaa gtaccggaga tggatcgaat aattcatcaa gttcagcatc aggaacagta
      661 gaagatgcct gtgcaactac ggatgacgct aattctggtt ctggatcaat tgggaaagaa
      721 ggaaaaatta acggtaaaaa aggtcaagta atagcgaata tgacttatga agaagctttg
      781 aaaaaatatg ggaaagcgat cacgctacca aaattcaaag aaccagattg gtcaacttca
      841 ccttttaacg gaatatatca aggtcagtgt acagagttga cgtgggctta tatgagccaa
      901 ttatacgatg ggaatcaacc gactactgga aatggtggtt atgtatggga aagctacaaa
      961 tcggcaggag caaaaataac gaacaaacca atcgtaggtt atgggtttag tgcttcggat
     1021 ggatggttat gggctgttgg agatgcaggg catactggtg tagttatagc ggtgtttgaa
     1081 gatggatcat ttctatgtgc taactttaat gttccaccaa cccaagctcc aacaagggga
     1141 gtttcggtaa cgttgattga tggagtggac ggatcagata atgtgcattt ctttagcggt
     1201 gtaggaaaag ctaaatttga aatcgaaaaa taaattggaa tgaaggacga tatagatgaa
     1261 tagtaaagtg aaagatacaa ttatcatttc tttgctttct ggtatgttat tagcactgct
     1321 tgttggaatc ctattcttca ataagcagaa tcaggacata gaagctgaaa aacacagtgt
     1381 gcatattgaa gataagctga cattctttaa agaggttaaa ccaaatgagg tggatggatt
     1441 acggaaagat ttagaaaatg atattcaaga aatgttgaat ggatcagttc aaaataagtt
     1501 tgcagatcaa tcaggtgaag aatttttgag aaaactattt attgtaacag gtaaaccacc
     1561 aattactgat aagagtagtg agaaagaaaa aatcgagtac tataagcctt ttacttttaa
     1621 gctaacaaac tttggaatta agacatctag tagtgggttg tatgaagcat tgaccgattt
     1681 tgaagttcaa tacaatggaa aaaaagtgta tgataccaca ttaaccatca cactaaataa
     1741 tgataagaaa gtgactgggg gtgcttttta tgcaaaatca tgaaatcact actgctaaaa
     1801 aggcgatcgg aatactttcc ataattgtga tcctttgttg tgtggtacta tttcaaaaga
     1861 aaaatgctta tgataataat gtcaaagaat tggaagacct taaaagtaaa caaatcgaag
     1921 tagtcaagaa taaccagaaa gtgaaagcta tgcaggaagc tcaatacaaa gaattgggaa
     1981 atgctgaaat taaggaacaa agtaggaaat tctttaattt attttacaac tggaattcat
     2041 gggaaaaata tacgaagaat atgaaggaaa tacaaaaaga atttcctcag atagtaaagt
     2101 ctagtcctgt tgatatttct ggcacgatgg taggcactgg taatagtcca atatctagct
     2161 atactgctga ttactataca acaaatcaaa aaggtaaaat ggttgaattg attacacaga
     2221 caagaaatac tccaacaaca gcaacaacaa caatttggcg tggagtaaca agcataggag
     2281 aaaatcaatt gttttatttg gaggatttag atagctatgt aagagcagag taaaataaaa
     2341 aaagagctaa cggtaccaac gttagctctt tgtgctgacg atgataacaa aacctaccga
     2401 aaaaggattg tttcatcaca aacacaaaca gaaaagaatt gataaaaaac ccgaacctca
     2461 gaatttttat taatcaattc atttagatta taataaattg agctgaacat tacaatacgt
     2521 atatgttgta aaacgtaaaa aaagtcaaaa gtaaatactt tcttaaaaag tcaaataaaa
     2581 ctattaaaga caatatatat ggtttatttg acataagaaa atggtctaga tgataattgt
     2641 aatatagaat tgtagactta aataaagcat aaaatacctt ttatgataag gtatagctct
     2701 taattttaag cagttttaga tataagattg aaagtaaagt gggggagaat aataatggca
     2761 gatcataaat tagaaaagtt attagaagaa acgtggaatc caaaagaatt tagtgaattt
     2821 tttatggaga attttgaaac tgacttagca gtgattgtta aagatgctct tagagagcaa
     2881 gggtatcctg aaactgcaaa ctatataaac attaatttta ctttgtatac tgaaaataaa
     2941 ggaacttggg atttttgggc tactttagca aataaggagt taagtgataa atcagacaca
     3001 ggaattcgta acttttttga atcaaacaga gatgattata tgtatgctaa caatcaaaat
     3061 aaattgaact ttagagtaga gtttgatgaa acacctgaag aatttataga aagacaaccg
     3121 cctaaagaaa atgtagcaaa ggtattggaa gatcgttgga attcagatga aatagtttca
     3181 acaattagcg aactaggcgg acaatatgaa ccgctagtag aagcggttag agaagagcta
     3241 agactgaata agtttcctga tgttcaaaat attgatgttt ctcaaattga aataaacgtc
     3301 aagatcacga ataaacttga ttatggtagt tgggcagata tagcattaga aaaatacatc
     3361 tactcaacgt taaaagagtt tatcgaaaat agaatggata tcatgtatct acagcatcca
     3421 cagtatttaa actttggtgt tgaaatagct acacctcttg aagaatggaa aatggagcaa
     3481 gggcttgact agcctttgct ccattttttt attttacaaa tgaatacagt tcgtcgtttt
     3541 taccaagaat gattgtttgc ccaactttga gattgtcagg tgatccttca gcggtttgta
     3601 aggagaacgt tccattgaac ttatcggata ccgttatagg tagctttaat tctgatcctt
     3661 cctcatcttt gagaattgct gtttttcgtt catcaaaata aacagtcttt attaaataat
     3721 ttttcgtagt gatctcagca gatttgtctt tttttgtagt agtaccagtt tctttagaat
     3781 tagaggtatt ttctacgact attccatctt tggcatttga gcttgtgttt atttgattga
     3841 atattttcag cgacaagata acgctaagaa tagtcaatat agaaacaagt gtaattaata
     3901 ctgataattt cctttttttg ttcatttttt cttaacatcc ttttttgttt tgattagtta
     3961 gatattcctt atctaacaag cctttttagg ctattaaatg aagataaacc ccaaaaaaat
     4021 attgcaatta tgacagtgca atggtactat tattatacaa taaattttgc gaaaggggta
     4081 ggtctaaatc gcaaacaaaa agaaattgaa cctagacaaa tacaacttgt ataccgcaag
     4141 ggaagcaagt ttgattttgg gtaaaggtag aaattatgtt acaaacataa tcaactatta
     4201 tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt gacaaaacct atattataac
     4261 ggaagaagga atagaagcat tgcgatcaca aacagaaaat aaaaacaaaa agaaggaatg
     4321 aatatgaaga gaaattcgct tggtatggtt ttattggcat cagctgtact tgcggggtta
     4381 tcagtaccag taacctcaca tgctgatgaa tggactgcaa acacaccgga tcaaatccaa
     4441 attacaacag gacaatcaag ttatgcgttc aaaaaaggag atactatttg gtcaactgta
     4501 gagaagctta aaagcttagg ttacaagact actgttgctg agtttgccga attgaacaat
     4561 gttgatttgg caaaaggaga acaacggact atcccgattg gacgaacttt caacatcagt
     4621 aaagatacat taacagttaa ggaagcagat ggcgaagtag ttagtcaaac tgttcaaaaa
     4681 gataaacctg atcctattga aaatacggag caagagaaca aaaaatccga gactacaaaa
     4741 gaatcggaac caacagcaaa cacttctgat aagacagaaa caagtgcttc aagtgttgat
     4801 ggagcaagtt catctaatac aaataattct acaagtgaga aagaagcatc acagggaaca
     4861 agtgaaagta gcactacgga agctgatact ggaacatcaa caaataactc acaatcaaca
     4921 tcaaatgatg caaatgaatc aaccagtaca tcttctagtg atcaatcaca aacaggttcg
     4981 agtgatgaaa ctgacagttc tacttcacca agtaaacccg aacaaccaac taatgttgtg
     5041 ctaggcgttc catttattag tcaaggtaac acaatgcttt gcgaaggaac atctttgcta
     5101 gaagcgttac attataaagg tgttgcaaca aatcaagatt taatgacttt tgttaaatca
     5161 atgccactat ctcctgacaa taatccaaat aatggttttt ctggtgaatg gagacacaat
     5221 gtggatggaa cttatcaagg tatgaatccg gctcctgtag tttcatgggg tgttgctaat
     5281 ggcggaaatg ttgtagatat ttctggttca tcggtacaag gattaaaaga tgagattgta
     5341 aatggaaatc ctgtagtagc gtgggtaact tatgcctttg aacctgctca acataaacca
     5401 atgttttggg gcgatgctat ttggaatcga catgtagtag ccgttgatgg atttaaagac
     5461 ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt ggattaatgc aggatcattt
     5521 gaaacagcat ataacgtatc tcaattagcg gtttcggtac gttaataaaa gatatcgttt
     5581 atatactaag tagttatata gtctttattt gtccttgctt aactacttag aaaacttgtt
     5641 gctcatggtg ggtagaacta aaaggggtga tgctatgaat actagagcgt tgaaacagct
     5701 tgctttcaac gaaaaaaatg attaataggt gtggtgattt taacttagga atgggcgcga
     5761 aggtcattac cacaccgaca tatcgtggct cattttcttc gcctaactgg tataaagaag
     5821 gagtaacgag agatgaagca tgaaaaaacg ataattttaa aatcaaaaat aatttattgt
     5881 tttgcgactt tagtaatgat gttatctacg cttataatgc ctttttcagc aataagagct
     5941 gaagcagatg tcacaaaacc gacaagtgca aaatttatag aaactggcgt aaaaacagac
     6001 ggttatatta gggttaatat gccgaatcat ccaaatgaat ggatgatttc aagtcaattt
     6061 aaagattctc atggaaatat tggatattgt atggattcag aattgccatc cccaacagga
     6121 agtggagccg gaagcttgaa gtacaaggga gctggcagtg atgagttcta tcgtatgttt
     6181 aaaggtggtt ttccatcaaa aacagcaaag gaactgggtg caggaaatga tacagaagca
     6241 tggtatgcaa cacaattagt ttcatgggtt ttagccggaa actttaaagt ttcacagatt
     6301 gtatggtcac atccaaacca taccgccgca gaaacagctc gtgtaaaaaa agcatttgaa
     6361 aaaatttatg actatgctaa gaatggaaaa gatacgccca acactgagtt ttctatcaca
     6421 gcttctaaga cagctgacga aggaaaatat cacactttca catataaaac agcatcaaat
     6481 aagactggta atgcgaagct cacttttaca tcagcaaaac cagctggtat gaagatttat
     6541 gatgcagatg gtaaagaaat tactaacaac actgtgaaat taaattcaag ttttactatt
     6601 aaagtacctg tgactacacc aagtggtact ttgtcattta aaggtactgc taatgttagc
     6661 acaaccaatc cttttacttt tgatggacga ggtgtgtatc aagatgccgt agttatgatc
     6721 actacttcag aaacaaagga ctctaaatct ttgagtgcta aatggacaag agctgttggt
     6781 caggtaacag ttaagaaagt tgatcaagat ggaaatgctt taaaaggagc ggagtttact
     6841 ctaactgaca ataacaatgc taaatctgtt aagacaaccg gagatgatgg gttagctacg
     6901 tttgatttgc aacaaggctt aacatacagc ttagaagaaa caaaagttcc tgaaggttat
     6961 caaggttcat ttaaaaagga tggtattact gttaataccg atggacaaga atttagttac
     7021 actgctacga acacaattaa aaaaggtaaa gtaaaagtta aaaaggttga ccaagatgga
     7081 aaaccattag ctaatgctga atttaccttg acggatgatt catcccaaac acaagcagaa
     7141 aaaacagatg aaaacggact tgcaagcttc gatattgtag caaacaagac ctacagcctt
     7201 gaagagaccg gagtaccaac tggttataca ggcgtattca agcaagaagg aattaccgtt
     7261 aaagatgatg gacaaacttt tgaatataca gctacaaaca ctttgaaaaa aggtttagta
     7321 aaagttcata aagtcgatca agatggaaaa cctttggcaa atgcagagtt cactttaacc
     7381 gatgaaaaag gtgcaacaca agtagaaaaa acagatgaaa atggacttgc aagcttcaat
     7441 attgtagcaa acaagacgta tagcctagaa gaaactggag taccagtagg ttacacaggt
     7501 gcatttaagc aagagggtat caccgttaaa gacgatgggc aaacttttga atatacagct
     7561 acaaataatt tgaaaaaagg tgtagtaaaa gttcataaag tcgatcaaga tggaaaacct
     7621 ttagcagatg cagaattcac cttaacggat gaaaaaggtg taaaagaagt taaaaagacg
     7681 gatgcaactg gtctggctac gttcaacatt attgcgaaca aaacttacaa cttagaagaa
     7741 acaaaagtac caacaggtta cacaggcgca ttcaaacaag aaggaattac cgtcaaagat
     7801 gatggacaag tctttgaata tactgctaaa aacactttga aaaaaggtgt gatcaaagtt
     7861 cataaagtgg atgatcaagg gaaagcatta gcaaacgcag aattcacttt gacagacgac
     7921 aaaggtgtaa aagttgtaca aaaaacggac agcaatggct tggcaacatt taatattgtt
     7981 gccaacaaaa cgtacaaaat cgaagaaaca caaaacccta atggttatac agggaaatgg
     8041 tctaaagaag gcatcaccgt tgctgacgat gaacaaacct ttgaatatac agtaattaat
     8101 caccttgtac ctgaaacagt aagaacaggc ggaatcggta catcagcctt ccttcttgta
     8161 tttctaattg caggattgag tatggtgcta ctgtattatc gagaacagca caaagcaaaa
     8221 taaaaaagtg ttaatggaaa agaagtaaat atgaaaaaag aaggctgacg tatgaaatca
     8281 aaaaaaattt tttcaaattt aatgctattt ggtactttgc taggagtggg attgggcgca
     8341 agctcaatct cttctagcct tggactaggg gggcaagtag catatgcaca acaaaactat
     8401 aattatcaag gaactggaac gattcatatc cataaagtaa caaatactaa tggggatggg
     8461 aaccctgtct cagatattga taacaaaggg aaagaaatag ctttaccaaa tggtgtaaaa
     8521 cctcttgaag gcgttgagtt ctcttatgtg aaacttacta aagatgaagc taaatccttg
     8581 ggatggacgg atagtgatac tacaaaaggt caacatattg atgatgctaa agcacgatta
     8641 ttactaaaca aaaaatacag tgaagtaagt agaatgaaga ctgattcaca agggaatata
     8701 gacataccta tacaagtaaa tacagacgat ttaacagata acttgtattt gattgttgaa
     8761 acaggaacag tcaaaggtgt agttgaagaa gcgtatccta tgattgttaa cgtacctgaa
     8821 ctaaacggat cagatgatca atactttaat tatgatgtac atgtctatcc taaaaactat
     8881 ttccaattaa actcagcaga gcttgataaa aaagacgaag atggaaaggc tcttgccgga
     8941 actgtttttg aattgcgaaa agatgatgga tctgatcagg ttgttggaac ttataaaaca
     9001 gacagcacag gtaaattaac tgtagatggt ttggaattag gtgactatta tttcctagaa
     9061 accgacttgg gaacagatgc taataaaaca acctatttac tagatggtaa aaaattcaaa
     9121 tttagcgtta aacgtgggga gactgctaaa gtagttcttg atgttaccaa ctacaaacga
     9181 cctgtaatta ctaaggtaat tgataaagaa accgttgata ctggcgaaga tgttcaatat
     9241 acattgaaac catcaattcc tggtaatatt ggtaattacg aaaaatatgt tgtcaaagat
     9301 gttctaggtg cagagttaac ttatgattca tcagaagtga tcgaaggaat gactttagat
     9361 actgattaca gtatcgaacg tgacggacaa acgacaaact atgtctttac tgaatcaggt
     9421 atggataagc ttcagaaaca atttgatgca ggggatcgct tctttacaat tttgatgaac
     9481 gctcatacta atagtaaagc taaaatggat acaccactta agaatgatgt ttcaacgatc
     9541 tacaacaatg gatatgaacc cgaagacgaa acaccagttg atcacgctta tacaatcatt
     9601 ggtgatcacc aattcattaa aacagatgga gatacaagca aagcattgag tggagcaaaa
     9661 tttgtagtga agaacaaaat tgatccgaat aaagttactt acctaaaaca agatccaaca
     9721 acgaaagctg ttacttggat tgcagataaa tctaaagcta ctgaattcgt ttccggatca
     9781 gacggtaagt tccaaattga aggcttgcaa tatacctatg atgtagattt tgataaacaa
     9841 ggtaatccaa caatcacaga taaaaaaatc aatgattatg ctcttgaaga agttgttgct
     9901 ccttcaggat atacaccaac tagaaatgat gttccatttg tggtatcgaa tgatgatgct
     9961 tctagcgaag taacagacat caagaacctt aaaacacctg aaacagttcg tacaggtgga
    10021 attggaacag tagtatttct agtggttgca ggtgtggcag gaacagggct atatgttgtc
    10081 tacaaacgag aacaacacaa agcttaatga aataaaattc cctagagtgt ttttggctct
    10141 agggtttctt tttaaaaagt gtgtgaaaag taggtatgag catgaaaaag aagaatatca
    10201 agttgatcat cttaaaaatt gggatgttgc tatgtttagg agtaggtcta tatcctgtaa
    10261 ttactcaggc ttatacctac taccaaactc aacaaacact aaactcctat aaggaaaaat
    10321 atgatgaagc ttctgaaaag gagaatgaag aagtcttcaa gagatattca gaagttaata
    10381 ggaaacgaaa agacacagaa caaacctcag tcacgattcc tgaagataat aacaatattc
    10441 cgtcaaatgg aaatcttcag aaggataaat taggtgaatt agtgggtgaa attcttatac
    10501 caaagattaa tgtggatttg ccaatttatg atggtgtaaa cgattttcaa ctatcagcag
    10561 gagcaggaat attaaccgga actgattatc caactggtgg gattggaaac cattctgtta
    10621 tatcagctca ttcgggcatt cctaataaaa aatattttac agatttagag ttgctcaaac
    10681 ttggtgataa attttttatt gatatttcag ggaaaaagct tgcttatcaa gtagatcgga
    10741 taaaaataat cctaccaacc gaatttgagg atttgcttcc tgtggcagga aaagattatg
    10801 taacacttat gacatgtaca cctgttggag ataattctca taggttactc gttagagggg
    10861 ttagcatacc ttaccaaaaa agtattgttg agaaagaaaa gaagaaagaa gatttaaagg
    10921 taaaggcgca gatgaactgg tatcctatcg tagttgtagg ggttttatta ataatagctg
    10981 tgatagtcat tattaaaaaa atagtagcaa agagacgagg aatgaaaaat gaagaataaa
    11041 atactacttt ttttgactct tatttgcact ttggttttaa caatttatcc tgcaaatcag
    11101 ctctatcatc aattcaaaac aaaagagaat gtggcggaaa tccaagagaa aattgagaaa
    11161 aaacaaactc cttcaaaaga ggaagtggca aaaaaagtcg aacaaataaa atcgaacagc
    11221 ggtaaccaag gattagacaa tactcagaaa acagacggcg gatcatttga tattggtgaa
    11281 gctgaaggaa aggtcaatcc ttacacagga gatgtttttt caaaaaatca aacgaaagat
    11341 tttgaaccat ctacctattc aggggcggtt ggttatgtct atattccaaa aataaatgtg
    11401 ctagagcttt tgtttctagg cgcttcacag gaacatttat atagaggtgt ggcacagatg
    11461 agtgctacta gtctaccttt atcaggtaaa ggtcatcaaa cagtaatagc aggtcatcgt
    11521 ggcggttata cgtcaccaca atttctttat gttgatcaat tagtagccgg agaccacatt
    11581 tatatagatt atcttggtga aaaagcagtt tatacggttt tccgaacaga cgttattaga
    11641 aattacggtc agaattcaac actgactgat attcctgatt tagatatgtt aacactttat
    11701 acttgtcatc catatcctca gaactacgat cgtttattag ttagaagtct aagagatgaa
    11761 aaagcaaaat acatcacgcc acaagtaaca gttggcaata ccgcatacgc tgatgccatt
    11821 gatagcgagt acgtttcaga taagacagaa actgttacat cttctaatga tttatttgag
    11881 gatgttgatc gtgccaacaa ggaattagat ggaaataatg atcttaatat tccggaacaa
    11941 ggtagcgtag agatgcaaga cagaacattt gctgaaaagg caagttcaat attcacagga
    12001 attaccaata aacatcagaa aatgctttta gtcatttgga ctgtaatagg agcattagca
    12061 agtctaaatg ttcttattct tttaatcaaa gagtttcgaa aagcataatg aagattataa
    12121 aaattgatag agtacagtaa aaagggattg atactattat tgatctcttt tttattttga
    12181 atagagctag attcactttt aaataaaatc aaaactgatc ttaattctaa agaaaaagtc
    12241 aagaaaaaaa tacaacaaat ttttttatca gaatatttat cagaatatta ttagaaaaga
    12301 taaactcaaa agcttaattt aatagggtta ttggttgtat ttagaaataa tgtatggaaa
    12361 agctataaag aaatagataa gatgtagaaa atcttatttg tgaatgaaaa acaagtcatc
    12421 gtaaaatatt aataggaggt gtagtaatgg tgggaatatc aaaaacagct ttagactcat
    12481 tagctaaact atttagtagc aggtttgata gtgcagaatt tgttgaatat ctaagccaaa
    12541 acgaaagtgg aaaaactctg atcgaaacaa tgaaaaaaga ctatttgaat gaaaatgact
    12601 ttccgacatg gacgaatatt gaagatgcta catgggatgt agtgtgtacg atatattctc
    12661 cttgggtaac gattgctgat tttgttttag ataaaattgg aggggataca taccctgatg
    12721 ccaaatctct tgctacaagt gactttaaaa cgttctatgg ttatctaata ggtctaatag
    12781 gcaatggaat agatttgaaa gttacgccaa aacaaacacc tgaagaaatt gaaaaagaaa
    12841 aaggagaggt tcaaatggct tatgcaaatg acagagaaaa agtaaaatta ttggaaggta
    12901 aattatctga agaagaactt ggaaaaatgg tgcaagaaat caatgaagac agcaactatt
    12961 ttcctgaatt gaaggtatat caaaatgacg aagatttctt taatgaaatg tttagtaata
    13021 gtccatggga agcagtgagt aaaacaaatt taggagaatg gaactttcaa gatgatctag
    13081 ttgcatttag caatgatggg tatttacgat catattcaga aagtgagtat agagctgaat
    13141 taaaaacgta tgaaacggat attctgatga gctatttaaa tctattagag agtggtcagg
    13201 gagagttaag agataaagtt gaagaactat taaaagaaga gctataggga ttatttcaac
    13261 ataaacacaa gcagaaaact accaaacaaa acgtaccatg caaaaagtcg gttaagccgg
    13321 ctttttgctc tattatgaga ggagatcaaa ccatgagtgc aacaaccgat atggctttag
    13381 aagcagtaat aaaagataat acaggaaatg tcgaaaatgt agaagacttt agcgatattg
    13441 caatcaatct ttcagcatgg atttcagctg taggagaaga aaatatcgaa tttgagatca
    13501 agaatatgga tgacttaaaa gcaaaatttc cttttatcgc ttcgaaacta agtcaatttg
    13561 ccgaggataa atcgtggaat gaattagtta gtttttccga aaaaatttat gatattacgc
    13621 aagaagatga gtgtgtttca tgggaaatga ataatcaata caagaaaatt gatgctttcg
    13681 tcaaatcacg tatagaagga acagcttttg agtttgaagg tatagatacc gcagaaaatt
    13741 ggttaaataa agaatccttt attaaaagta tagcggatga tcttgaaaaa caatggtcta
    13801 aggaagacta tgcagagtac ctaaaaacag aaagagatac agaggtgaaa gaaaaactca
    13861 ctgaggtatg tttggatgaa catattgatc ccaatatcgt aaaacaagaa gactatgaag
    13921 tggttattga taaagttcct actgattttg aggaagttgt aaagtatata gttgaagaag
    13981 cattatcgca atactcttca gatgaggaag tggacgaaga aactgttcgt accagagttt
    14041 ggcaactggt ttttgatgac aaagattata aatatttgag tgatcaaaac tcggttaatt
    14101 ggagtgtacg attattaatt gatcaaaatg atattgaata aaatagagct gaaaagctct
    14161 atttttttga taaaaaaact agctatttta ttaaaaataa tttggttcaa aatttatttt
    14221 tttaacttga taattttttg tcatcctttt ttaaattggc tatatatcaa ggtttatagt
    14281 gggaacaaat aaaaatacat ttgacaaaat ttacaatata gtttattata tatttatctt
    14341 attatgataa cacaataaaa ggaatgatgg tatgaaaaag aagaagttta gtcgatcgtt
    14401 gttaggaatt ttggttttag ttgcggttgg tcttagtggc aagggttact ttcagaataa
    14461 cagcactcag gaacatgatt ttcagatcaa aagtagtgtt caggtaggta ataaagcaga
    14521 cagtgaatca aaagaatttt tagggatacg ttatgatcca aacaaatatc caaataatat
    14581 cgtaaacatt aatgatggga aatatacact gacaaaagaa gaccatgaag cattatcaga
    14641 aaaaggcagt caaacctttt gggtaaatta tgcagattta gatcaacttg gaagagcagg
    14701 aaatgttgaa gcgttgatca cctatgatgc tgtaaattct cactcatcta aaaatgtgga
    14761 acgtccatct ttcaaaagtt ctgtccatat tgcaggagaa tacaaggatg gagtatttga
    14821 tcaagagaga gatacttggg tcggcaaaga ttcaaacaat gaaatcactc agctagaagg
    14881 ctataaaggt tatctctata acaaaagtca ttcattagca tggtcgctag gaggagatat
    14941 ggaggtacac aatttaacac ttggaacgag agcgcaaaat gttgggaaaa atgatcagaa
    15001 tggagggatg gcttactcag aaacgattgt acgtgatgca atctataaaa ataagaatga
    15061 aaaagtattg tataaagttg atccaattta taaagggaat gaattggttc ctcgtggttc
    15121 tcatgtcgaa gctttttcag ttaacgataa tggaaaaacg gtaaacctta atgtttatgt
    15181 atttaacaat caaaaaggaa tcaacatcaa ctatcaaaat ggtacttggg taagagatta
    15241 gaaattgaga aggtgaagaa ttggaaacaa taagcatcaa tggattagat aaatcagttg
    15301 caaatcgaat aaaaaaagga aatggctact atcttctgaa gaagggtaaa tattcagttg
    15361 ctagagagtt agaaacaatg agtatcgtgt atgtagctaa gaatcatttt gacgtagaac
    15421 aggctatttt tgatccagat tctgaaatag ggaaatgtgt tgaattatca gagaaaactg
    15481 ttagtacaaa aggggcgatg atcattgaga gagaaatgtt agacgaaatc agagtaatca
    15541 ttcctaaaga tacgcagatg tgtgaggaca gcataagtga cgattaacca aaatcagaaa
    15601 gaaaataagg agatattcag atgagcgaaa aatccattga attaaaggaa tataagcgaa
    15661 gcgatggaga aaaatattat cctgtagcca atttttctaa atcagtatat ctggttggac
    15721 gtgcattggt agttagtgca attggcatat atattttgca ttgggtaaca aaggttgtac
    15781 aactgcttgt agatacctta gtaagtattc tcgtctcgtt aaatgatgtt aacggaacag
    15841 atgatttagg attaaaagag atgtttaaag agttactcaa tgcgtttttt ccaaattatt
    15901 taagttattc atataatgta caaagctttt ttgcaaagct acacttggta gatatctcta
    15961 ttttagtatt agaagcagga acggtattct ttgtcggata tttggttact ttgatgttca
    16021 ggcatcacta tggagaacaa agaccattct ttgatgatat ggaaagtaga aggttgaaaa
    16081 gagaagctct agaagctatg gatgtgaagt atgatccaaa caagattata ggagatgaaa
    16141 cgaacaacaa aaaaagaaaa cgaaacaaaa cgcaattaga atccgatgtg ctatcaacgc
    16201 ttaaaaatat caaaatttct gttcatacaa gacgtgatct aagtgtcaat gaagatattc
    16261 gagaatatat cgtaaaaata ccgcaaccaa aagggaagaa agttagagaa acagttttac
    16321 gacaaattca agatttaggg tcgatcctga caagagcaac aagagggcga gctaaatttg
    16381 gggaaaaaga agacgaagtt tcaggtgatt acttcatctt cagagcatca gtaaacattg
    16441 aaagagaacg taaggaaaga gtaagtacca aaaacaagga aacggaaaaa tcaacagcat
    16501 ccattgcagt ttcaaattca caatttgctt ttccaatcaa tctctttgca gataactcca
    16561 aaaatatcca aaaccaaacc gaaaaagcta gaaaatatgc cgaaactcaa aagcaatctt
    16621 tagaacttta tctatcttct gctgacgtac ctgcaagtct aacagatatg cacgttggga
    16681 atacatctat tgaatatttg tttactttat ctcgtaccag aaaaattcaa ctaagctcaa
    16741 tgaaagaaga aattgaaact tacttgcata tatcaggtgt aatcgtgcgg atcaacgcag
    16801 ggcgtattca aattacttta ccattaggag aaaatgatcg tattccaatt gatgtaccat
    16861 caatgatcaa tagcgtgttt tagaagacat cacaaataga aacagaaaat acaaactacc
    16921 agttcacaaa cagaaaagta ccgagaaaag agaggaatag aaatggctat gaaattgtta
    16981 gagaaagtca aaaatagagc cgaaaaagct gaatctattt tgctagataa agaacctaat
    17041 ataaagctta atggaaaatt cagtgtcaat atgttgaaca caaaggctaa agacccgaca
    17101 gatgctattt ttggaattaa gcctgatggc acaattgctc atttttgcat cagcgatacg
    17161 ccacacatgt tagttgccgg aacaacagga tcagggaaat cggtattact gaatgaaatt
    17221 cttgttacag ctatctgtca tgcaatgcct gatgaattaa agctagggat agtagaccca
    17281 aaacgagtag agtttgggcg ttataaaaaa ttaccctata tgcttgctga tccaattact
    17341 gatatggatg aagcttatga attttttgaa taccttgtga tcttaatgaa tgaacgctac
    17401 aaattgatgg aaaaagcagg cgttcaaaat atcacacaat ataacaaata cgctgaaaaa
    17461 catggaatag agaaatttcc ctatgtgatc ttattggttg atgaatattc gcaattagtt
    17521 ggaacacaca aagaagttga agggctgatc gttcaattag gacaaatggc aagggcggca
    17581 ggaattcacg ttattcttgc aacacaatca cctcgatcta cagtcgtgac aggtattatt
    17641 aaagcaaatt tcccatctag agtctgcttg atggtagcaa gtgatttaga aagtaggatc
    17701 gtattagatg agggtggtgg agaaagccta agtccaaagg gagatatgtt tatcaagttg
    17761 aatggaggaa acaaaatcag agcgcaagga gcctatatca gcaacgagga aattgaagcg
    17821 atctttgatc accttagaaa tactatgcca gaaccagaat atgttgatta ccgagcaatc
    17881 gtagctgaat ctcgtggtga gggcggtgaa gacgaggatt ttggtggaat tggtaaagct
    17941 cctgtaagaa aatctgctaa aagaaatttg aacacacctt tagcaaattc atcatctaaa
    18001 gagccatcaa ttgaaaagaa gagtacgttt gctctaaaac ttgaaaaaaa caaagaagaa
    18061 aaagaaaaac aaggagctaa aacactagaa aaacttcaag ccgaaaaatt agaaaaagtt
    18121 ttaaaagcat tagataaagt tgatcctgaa ccgcaaacct caaacaacac gaataatgaa
    18181 cagccgattt catctaaaac taaaccgaaa actactgatc cgaaagtttc agtaaaagtg
    18241 accatcacaa ccgataattc tgagaagaaa caagtaaacg gaactacttc acctatcatg
    18301 caaaaaatgt taaaagccaa aaaacaggtg cataaatgat cattgtcagt tcttttttga
    18361 ggtggcttac gccatataaa tatacagatg aagtagttgt gaaacgagct aagaaaaaag
    18421 ataaaaaagc ggtgttaact cctaaacaga tgcttgatca tggaattttg ggtgtgaagg
    18481 ttttagaagt gaataaaccg tttgaatcaa atagtgcaag agaaaagttg cttggcgctg
    18541 aagcagatgt ggcatatttt tatcatgaca aaagcaagca tcaaatagct attaaattca
    18601 atccaaaatc ggcatggcgg tatcaagcaa gagatgactt gaaacgtagg aaacttagac
    18661 caatgctcta tccagaagga aaagaagcat ttgatagaac ccacatcgtt cccatcggtt
    18721 ttcatggtac agaatcagat aatcgcctat tagtaggttg gaacagcaat caaaatagag
    18781 ttcagatcaa taaatttgag aagaaagtaa gaaaattcaa cgaacgaaaa acaatcctat
    18841 ggtttgcaga tatttgtaaa cagccggacg gaacagcaaa gtgggaagcg gtgatatttg
    18901 acgaaaatgg taaaaaagta atgtctgaaa catggcatga taaagctaaa tttgtttggc
    18961 agtagaggtg aaaattgtgt taactattct aaaactatat agagagggat ttaacgaaaa
    19021 agtaagtatt ggtgaatcca ttgaatatag aggtgataag tatattatta ttcgcattct
    19081 taaaattaac aaacattatt ttagcaaatc tattagttta acagttagag ttctagttca
    19141 gaaagtcgga gacttgagtg attattataa ctgcaaaaga gaagttattg tggataatcg
    19201 atacgaccaa tcaaaagatg ataagcgaat aataaaaata ggagatattg tctttcctga
    19261 gaaagggata gcatatgaag tagtgtctat caatagtttt cgctatgaat ttgttgattt
    19321 ggttgtcgaa tattctgttc gattgatcgt tccgtggagt aaagaagaaa ttaatgaagc
    19381 attaaaagat gaacgaaaaa gtacattcaa agttttagaa ggtgaaaaaa agtgaaaaca
    19441 gaccttacta gacaagctga gaagtgttta tggcatgaaa caaatcgcat gggtgtattt
    19501 ggttgttttg aagtaactat cggttgggat ggcagagaaa gagtagatta tataacttat
    19561 tcagtagata acacagttcg atgttatgag atcaaagtta ctttatccga tttgaaaagc
    19621 aaggcgaaac agacatttat cggcgattat aactatttag tcgttactag tgatttgtgg
    19681 gagaaaatgc tacaagacga cacactcatg cgtaaatatt ataaacaagg cgtatacgtg
    19741 tttaatgaaa aatatcctag catgataagt gttaagaaag caaggaaaca aaaagtttcg
    19801 ctaggtacaa gatcaactgt attagaaagc atggttagat cgatgaatcg tgaagttaag
    19861 aaattatata aagtaaaacc attttgggag atagtagaga taccggaatg atgagtaagg
    19921 aatattatat ctattcatat atagtattaa ctgattatga ggaggataca gcaaatgaaa
    19981 aattatgatc cgaatatgcg atggggtaca catactctta aagtgtcttt tcaacggagg
    20041 ataagctaaa tgaataaaag aagaatactg tggcttattg caggaatgta tctttgcata
    20101 gcagtatctg ggtttactat ttgtcggat
//
LOCUS       DACDIP010000048        19734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDIP010000048 DACDIP010000000
VERSION     DACDIP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.048"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            73..273
                     /locus_tag="IV257_001947"
     CDS             73..273
                     /locus_tag="IV257_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8815052.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            420..3209
                     /locus_tag="IV257_001948"
     CDS             420..3209
                     /locus_tag="IV257_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8815053.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            3256..3783
                     /locus_tag="IV257_001949"
     CDS             3256..3783
                     /locus_tag="IV257_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8815054.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3804..4994
                     /locus_tag="IV257_001950"
     CDS             3804..4994
                     /locus_tag="IV257_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8815055.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5034..6332
                     /gene="asnS"
                     /locus_tag="IV257_001951"
     CDS             5034..6332
                     /gene="asnS"
                     /locus_tag="IV257_001951"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8815056.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7509..8183
                     /locus_tag="IV257_001952"
     CDS             7509..8183
                     /locus_tag="IV257_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8815057.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8180..8521
                     /locus_tag="IV257_001953"
     CDS             8180..8521
                     /locus_tag="IV257_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815058.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            8551..8910
                     /locus_tag="IV257_001954"
     CDS             8551..8910
                     /locus_tag="IV257_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8815059.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9113..9292
                     /locus_tag="IV257_001955"
                     /pseudo
     CDS             9113..9292
                     /locus_tag="IV257_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            9434..11515
                     /gene="cadA"
                     /locus_tag="IV257_001956"
     CDS             9434..11515
                     /gene="cadA"
                     /locus_tag="IV257_001956"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8815060.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            11826..13292
                     /locus_tag="IV257_001957"
     CDS             11826..13292
                     /locus_tag="IV257_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8815061.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            13907..14446
                     /locus_tag="IV257_001958"
     CDS             13907..14446
                     /locus_tag="IV257_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP8815062.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            14520..14960
                     /locus_tag="IV257_001959"
     CDS             14520..14960
                     /locus_tag="IV257_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8815063.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            14973..15182
                     /gene="copZ"
                     /locus_tag="IV257_001960"
     CDS             14973..15182
                     /gene="copZ"
                     /locus_tag="IV257_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8815064.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15182..17368
                     /locus_tag="IV257_001961"
     CDS             15182..17368
                     /locus_tag="IV257_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8815065.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            17388..19559
                     /gene="copB"
                     /locus_tag="IV257_001962"
     CDS             17388..19559
                     /gene="copB"
                     /locus_tag="IV257_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8815066.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6591 a         3440 c         4304 g         5399 t
ORIGIN      
        1 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
       61 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      121 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
      181 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
      241 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
      301 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
      361 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
      421 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
      481 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
      541 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
      601 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
      661 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
      721 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
      781 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
      841 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
      901 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
      961 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1021 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1081 tgcagaaaaa agaagtggaa ctattcatat acgtccatta tggcgagcgg acatatccaa
     1141 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     1201 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     1261 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     1321 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     1381 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     1441 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     1501 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     1561 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     1621 tcgcgcacag aggcactgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     1681 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     1741 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     1801 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     1861 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     1921 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     1981 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2041 gcaagcaacc taacggacag tatccagaag aagtcatgac gacaaaagag atgttggatc
     2101 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     2161 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     2221 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     2281 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     2341 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     2401 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     2461 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     2521 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     2581 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     2641 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     2701 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     2761 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     2821 tgtttggcgc tgacagtttt tgggaaggtg tagatttgcc tggagatgct ttgcagatcg
     2881 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     2941 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3001 tgaagttacg ccaagcatta ggacgcttga taaggacgga gacagatcgt ggcgtattgc
     3061 ttattttgga tcgaagacta gttactgcaa aatatggtgc acggatcatc catgcattac
     3121 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggat atccatgaat
     3181 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     3241 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     3301 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     3361 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     3421 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     3481 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     3541 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     3601 aatgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     3661 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     3721 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     3781 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     3841 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agccaaaggc agagatgtgc
     3901 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     3961 tcgaagcaat ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc
     4021 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4081 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4141 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     4201 aactggcaga aggacttcct gtctttttga aaggagaaga atccaatagc tggaaagtta
     4261 ctgttgagca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     4321 catcgaatcc gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg
     4381 cagtaaaaaa tgatattttg atagttgctg atgatattta tggccgttta gtttataatg
     4441 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca
     4501 tcaacggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggaa
     4561 atcctgtgat catcaatgga atgatcgctg ttgcttccca atcaactagc aatccgacag
     4621 cagtcagtca atatgcagct gttgaagcat tgaaaggcga gcaagataca gtggaagaaa
     4681 tgcggaacgc ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccgg
     4741 gtgtttctct tgaaaagcca caaggagctt tttatctttt tcctaatgta aaagaaacgc
     4801 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     4861 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     4921 ccactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     4981 aaagtcaaaa ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5041 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5101 tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca attaagagac ggaacagcct
     5161 ttttccaagg tgtagttgta aaaagcgaag tgccggaaga agttttccaa acagctaaaa
     5221 atctgcacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agccgttcaa
     5281 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     5341 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctac
     5401 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     5461 aattctttaa tcaacatcat tttgtcaaaa tcgatccgcc gatcttgact ggttccgcac
     5521 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     5581 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     5641 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgattgaa ttctggatga
     5701 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     5761 tagcattctt agtacaaaat gttttagatc attgcgaata cgcattagat gtactaggac
     5821 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttatgatg
     5881 atgcgatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     5941 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6001 atccaaaagc tatcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa
     6061 tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6121 caacggacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     6181 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     6241 gccttgaacg aacagtcact tggattgctg gtatcgacca tgtgcgtgaa gctattccat
     6301 tcccacgttt gttgaaccga atttatccat aagaggtgat gaaaaagctg ttctgcatca
     6361 agtaattaga acagccaagc aaaaacagct gttcatgttt ttgaattggc gggacttatt
     6421 accgcagatg taagcttttg aataccgttt agtggagagg ttgtgaaaaa gctgtttagt
     6481 ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac atttttgcat aaatttggct
     6541 taataccgag aaactggctt ttgaacaccg tttaaggaag aggttgtgaa atcagtgcct
     6601 tgacctgagt attagagaaa caaaacggaa aaatagcttc tcatatttta ccgttttttt
     6661 agctaatatc gagggtcagc tgattgaaca ccgtttatat caagaggttg tgcgcctgcc
     6721 gttcagctcc gagtcacaag gagtaatttc aaaaaacagt ttttcatgtt tgatgactat
     6781 tgtgacttgt gaccgaggag ttggccggag agcaccgttt attctaaaaa agaactgcga
     6841 actctcgcag ttcttttttt aaaatacggg ataaaggaag tttccagcag acaagagaag
     6901 aaagcaaaag aaattgctat ttgattcaca aggttctata acgaaatcgc aatcaatcaa
     6961 acatttacaa agaaggtaat tcaaatggaa atctgatttg atgatgcttc tgctgttgaa
     7021 ataaagcagc atctagcacc tgaaaacaaa ctaatagtaa cattcgaaga tggagtaggt
     7081 ccgtattttc agcatgcaat aatccatatg taagttcaat tcacccttga aatcatcgga
     7141 ccagataaag ttatgaccaa tctatcccaa gcaatctaga agagatatta gtaaagggac
     7201 atttactaga agtcttagat gagcctataa atatatgttc tgatggcgtc atcagtggtc
     7261 tgattgacgg aagcttaggt gtgatcgatc tctctggacc aaacaacgaa ggaatcaagg
     7321 ggaatccggc acgttaagaa aaaaatagca taaaaaacta gacacttgat gagcgttcat
     7381 tcaattgttt gaagggacta ggctggtgtc attttttata catatatttt ctctttcgtc
     7441 ttgatagggc ttatgggata atataaggaa gaaaatcatc gttttattca aaaaggagtt
     7501 tttgacaaat gaaaaaacgt atattattta tcggtgatag cattacagac gcaggaagag
     7561 accgagcaga agcaactgat ctaggtaaag gttatccact tttgattgcc ggaaaactaa
     7621 tctcccaata tccaacgatg gaatttgaat gcatgaaccg tgggatagga ggagacaaaa
     7681 ttgcagatct gcaagtacgc tggcaagaag attgtttagc tttacaacct gactatgtat
     7741 cgattttgat cggaatcaat gatacctggc ataatactag cgatcaggct atattcggaa
     7801 ctgagaaatc tgctcagcaa tttgaatccg tttatcgaaa attactaaat gaacttcaaa
     7861 cagtgggaat ctgcaacatc atcttgatgg agccgtttgt acttcctata accgacgacc
     7921 gtcatggctg gcgtaaagac ttagacccta aaatccaaat tgttcgtaaa cttgctaagg
     7981 aatatggcgc attgctggtt cctttagatg gtatattcaa tgctttaggg attgtttatg
     8041 gtttttcgaa atatacaaga gaagatgggg tccacccaac gattggcgga catgagatca
     8101 ttgcacaaag ctggatagat gcaattgccg cagtcgattt ttttagtgga gataaaaatg
     8161 agcgaaagaa aagtgagaaa tgatggaaat acggtgggca gtaaaagaag atctacctca
     8221 aatcaatcag ctgattactg atcatccaga atcattcgta aaagcaaaaa cggcagctga
     8281 cctaaatgat aagaataaac tattgctcgt tgctgtaaca aaagaaaaac ggattatcgg
     8341 atttgcgcaa gcggacgtgc aacagtcaga agaacgattg gatcagcgac catcaggaaa
     8401 actaatccaa cacacattag taacaaaaga aaatacggaa gaaatccaaa aaaaatttgc
     8461 agaatatttg atactggaag ctaaaaaaag aagaataaaa tcattttttg cccagtttta
     8521 atcaaaaaca gtaatagaag gagaaataca atgagttgtt taggaaatgt catctggttt
     8581 atattcggcg gactaatcgg tggtcttagc tggttagcag cggggatact ttggtgtatc
     8641 acgatcatag gtatcccaat cggtatgcaa tgttttaaga tcgcctccct ttcttttgcg
     8701 ccttttggca aagaagtaat ttatcaccag tctggcatgt cattgattgt taatatcatc
     8761 tggctgatcg tttcaggcct accaattgct ctcgcacatg tcgtcagcgg catccttcta
     8821 tgtatcacga tcatagggat accttttgcc aagcaatctt tcaaacttgc tcgattggcg
     8881 ttgatgccat tcggtgcaga aattgtataa aagagaataa taaaggttag ataaagcata
     8941 gagtcaggtg ggatacatgt atttcacctg actctatgcg ttctcctgta taagttcttc
     9001 catgtgactt ttacgtcttt tatttatatg gggttactgt ttatggaaag ttgaaataat
     9061 tttcattaaa gaactctttc tatatttttg aaagcgtagt ggagcttcga atatctggga
     9121 aggacatttt gcagggattg aaaaaagata ttgtcctatc gaaaaattaa tgaatttaga
     9181 caaattgcca gcaacaggct tcaaacttag ttgctatcct gtcaagatca agaaggcaag
     9241 tgcgggttgg ataagagcag tggcaatgat cgaaaagaaa aagaaagaat aagccaaaac
     9301 atctgtatgg aagaaatttt ccgatgcaga tgtttttcat tttttctttc ggataccttg
     9361 acatatgaat aaccgttcat ttataattag ttatgaagag atattcatat gttgaggcga
     9421 ggaggtcatt aaaatgaaaa gttacagatt agaaggttta gattgtgcga actgtgcaat
     9481 gaaaatcgaa aaaggagttc agaagatcaa tggagtcaag gaagcaacgg taaacttcac
     9541 ttctggaaaa ctaacgattg acgcagagga agatcatttg gctacgattg aacaagaaat
     9601 aaaaaaagta gtgaaggaat tagaaccaga tgtcaaagta acagaaatag ataaggaaaa
     9661 agtatctgaa catggaaatg aaaaagagag aaatacgctg ttccggattt tattttccct
     9721 tgcaggtatc gctttattgc tattatttga ttttaatgaa ccaattcgct tgatagggta
     9781 tttattgatt tatctgctga taggatatga tgtagtgaag aaggcagtga tgaatatcgt
     9841 caaaggaaaa atctttgatg agaattttct tatgtctgtt gctacgattg gcgctatgtt
     9901 gatcggtgaa tatccagaag ccgtagcagt gatgctattt tatcaaatcg gcgagtattt
     9961 ccaaggattg gcagtcagtc attcaagaaa atcgattcgc gaattgatgg cgatccgtcc
    10021 agaaacagct cacgtgcaga cgacggaagg attgatgacc gtgaaccctg aagacgtgca
    10081 gatcggtcag tttgtacttg tgaagcctgg tgaacgtgta ccgttagatg gaacgatcat
    10141 cgaaggagaa tcattagtcg atacgtcagc acttacagga gaatcagtcc ctaaatctgt
    10201 atatgttggt gaaacagtat taagtggatt tatcaataaa aataaaccat ttttagttca
    10261 agtagaaaaa tcttatgaaa attctactat cagcaaatta ttagaattag tagaaaatgc
    10321 cagcagtaaa aaagcaccag ctgaaaattt cattactaaa ttcgcgcgct attacacacc
    10381 agtcgttgta ggattggccg ttttattagc tgttttacct ccacttgttg tttctggagc
    10441 ggcgttttct gaatggatct atcgagcatt gacattttta gtgatctcat gtccatgtgc
    10501 attagtgatc tccgtacccc ttagtttctt tggcggtatc ggaggagcaa gtaaaatcgg
    10561 tgtattagta aaaggttcaa attacttgga gttgcttgca caaactgaga cagtggtatt
    10621 cgataaaacc ggcacattaa cgaaaggtga cttttcgatc cagacgatcg atacaaaaat
    10681 cgatccaaag atattcatgc aatatgttgc gagtgcagaa cagttttcta cacatccgat
    10741 tgctcaatct gttttagaag gctacgcagg tccgctttta ccaacagcta atatccaaga
    10801 atttgcaggt gaagggatct tagctgaagt tgatggcaaa caagtattag caggtaatca
    10861 caaattgatg gaaagatttg aaatctcttt tccttcaagt caagaaatcg gtacattgct
    10921 ttatctagcg atcgatcaaa gttacagtgg ttatcttgtt attgcagata ccttgaaaga
    10981 agatgccgta gacgcattgg ttcaattaaa aaaggctggt gtgaagaaca cagtcatgtt
    11041 gacgggcgat tcgaaaaaaa ttgctgatca tatcgggaag caagtaggcg tggacaagat
    11101 atatagcgaa ttgcttccag aagataaagt gcagcggtta gaggaaatct tacaaagaaa
    11161 caataaaaag accgcatttg tcggagatgg aatcaacgat gcaccggtgc tagcaagagc
    11221 agatgtagga atcgcaatgg gtggattggg aagcgatgct gcaattgaag cagcagatgt
    11281 agtcatcatg aatgatcaac catcgaagat tgcagaagca atccatttag caaagaaaac
    11341 attgaaaatc gtcaaacaga acattgtctt tgctattggg ataaaaattt tggtacttgc
    11401 cttaggtgct tttggttttg catcaatggg agatgcagta tttgcagatg ttggggtgac
    11461 agtattagct gttttgaatg ccatgcgaag cttgcatgta aaaaataaac gttgatcaat
    11521 gaagaggacg aatgaacgtc ctcttttttt tatatccttt tcttaatgaa aaatgtaaag
    11581 aaaatagata ggattataaa ataactaatg tttttaattt tgcagaaaat tcaaaatagc
    11641 ttgaaattca ataaggtaaa tgaaataaaa gaaaataact tatttttttg atggaaaaaa
    11701 tatgaaaaaa cgatgtgtat taaataaaag aatacacaca taaaataaca ctgtcttgta
    11761 agtttgttaa aaattgaata tgattacatc tggataacaa atttttatca taaaggagag
    11821 aacgaatgaa caacaaacaa ttacgctggt ttactgttgc gttaattgca tttaacatgg
    11881 tttggggcat ggggaatgta gttaataact atgctcagca aggagtatca gttgtcacct
    11941 cttggctatt gattttggcc atttatttta ttccatacgc attgatcgtt ggacaattag
    12001 ggtcagcctt caaggatagt aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta
    12061 ataaacgact ggcatactat gccgcttgga cttactgggt cgtccatatt ccttatttgg
    12121 cacaaaagcc tcaagcaatc ctgattgctg gcggttgggc agtccaagga aatggaaata
    12181 tcgtaaatac gatgtcagta caaatggtag cagttatttc acttattata tttcttgcct
    12241 ttttatattt atcaacaaaa ggactttcta ctttgaaagt gattggcgga ctggccggaa
    12301 cagcgatgtt cgtcatgtcg atcttattta ttcttttagc tgtaacggca ccgttcatca
    12361 atccaacgat ggaatttgcc acaccacata tgaatcaagt gaaaacatat attccaacat
    12421 ttgatttcag ttatttcaca accgtttcga tgctagtatt tgcagttgga ggagcggaaa
    12481 aaatatcccc ttatgtcaac tctacaagaa atcctgcaaa ggaatttcct aaagggatga
    12541 tcttcttagc agcaatggta gggatttgtg ctgtattagg ctcagtcgca atgggtatgc
    12601 tgtttgctag cgataatatt cctaaagact tgatggcaaa tggggcatac agtgcttttc
    12661 aaattttagg taattactat ggtatcggta actcattgat gatcatttac gcattgacaa
    12721 acatgattgg acaaatctcc gcattagcct tttcaattga tgcaccacta aaaatcttgt
    12781 tagcagatgc ggatccagaa tttgtaccaa actggctacg taaacgtaat cgtaaaggta
    12841 cattggtgaa tggttatctt ctgacaggta tactagtagg aatcattatt ttgttgccga
    12901 tctttggtat caaagatatg aatgaattag taaaatggtt gacgaacttg aactcagttg
    12961 tcatgccaat gcgttaccta tgggtgttct tggcatatat gatgttgaat aaagcttata
    13021 agaaattcaa ttctgaatat aaatttgtta aaaatccaaa aatcggtttt atctttggtg
    13081 cttggtgctt tgccttcact gcgtttgcat gtatcctagg aatggtgcca aaagtagatt
    13141 atactgctga tcctaaagca tggtggttcc aattattcac gaatatcatc acaccaattg
    13201 tcttgatctt attaggtatg atcttgccag caattgctcg tcgagacaaa aacaaagaaa
    13261 tcactgaacc agaaacagtg gttcagcctt aaaatagaaa tacttgttac aagaggttgt
    13321 gacagaagtg gtcagcttca agaaataaga aggaatttac gaaaattgct tctcaaattt
    13381 ttgtgaagtt tcggcttgtt tttcgcagaa gctacttctg gaacaccgtg aatcagaggt
    13441 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcccctcata
    13501 tttttcgttt tgctgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    13561 aggtagtgag cctgccgttt aactccgagt cacaaggagc aatttcaaaa aacaacttct
    13621 catgtttgtt gactattgtg ccttgtgacc gaggtgttgg caggagaaca ccgtttaagg
    13681 aagaggttgt aaaatcagcg atttgacctg agtattagag gaacaaacga aaaaatagct
    13741 cctcatattt tgccgttttt tgagttaatg ctgaaggtca gctgattgaa caccatttat
    13801 tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgttttttt gcggcgttat
    13861 aagtggtatg atcgaaccaa attccaaaga gaagtgaggg agaagaatga agatcatcgt
    13921 gattggacaa agtgggtcgg gaaaatcgac cctcgcaagg aagatcaaag aaatcaccga
    13981 ctttccgcta ctgccgctag accttttatg gcatacaaca gactacagta ttcaggcaaa
    14041 aaagtggttt ttgcaagaac agcaatcgtt tatggtatct aatccgtcat ggattgttga
    14101 aggaaattat acttctacat tagaggaaag gattaaagaa gcagataaga tcatttggct
    14161 taaagtgccg cgttatctcg ccatctaccg agtagtttgg cgttctctaa aaagaaagat
    14221 aaacaaaaaa tctcggccag atatgcctga atcattttca gagaaactaa acagggaata
    14281 cttagaattt ttatctttta tctggcattt tgaagaaaac aatgaaccga aaatacagca
    14341 gctgatccaa gtagctaatg caagaaataa actgatcatc cttaaaacaa gacgagagaa
    14401 acaagaattt ttaactaaat tgaaaataga gagcaaaaat aattgacagt ttttgtttac
    14461 aagcgtaatc tgtattcata agctaatgat tacatttgta aacgaaaggt gtgaaataaa
    14521 tggaaaaagt caaaacgcca gtaaagatca gtgattcaga gtgggagatc atgcgtgtta
    14581 tttggacact tggacagacg acagcccaag aaatcacgca gatcttagct gattcgatgg
    14641 attggaaacc agcaacgatc aaaactttgc tgggacgttt ggtaaaaaaa gaagtgatct
    14701 ggacagaaca agaaggaaaa aaattcattt atcatccagc tgtctcggag atggaaacag
    14761 ttcgcagtgc gacggaaaat cttttttccc atatctgtgc aaaaaggatt ggagagacga
    14821 ttgcggatct agtagaagaa gccactttga ctcaagaaga tatcaatgag attttgaaac
    14881 aactggaaca aaagcaacca gtagatacga tcgaatgtaa ctgtatccca ggtcaatgtg
    14941 aatgtaaaca acatcaatag gaggaagaaa aaatgaaaca acaattttca atcgaaggta
    15001 tgagctgcaa tcattgtgca gcaagagtag aagaaacagt aagcgcactt gacggcgtgc
    15061 aaaaagtgaa agtaaatttg aaaaaagcaa acggtacggt caagtttgat gaaacaaaag
    15121 ttcaatccga aaagatttgt caagcaatca atggattggg ttataaagca gaggtgattt
    15181 gatggcaaca gcagaaacca aaatggatac gttcgtcatt acaggtatga cttgtgcaaa
    15241 ttgttctgct aggatcgaaa aagagctgaa cggacaacct ggtgtggtca atgccacagt
    15301 caacttggcc actgaaaaag caagtgtcaa atatgaaggg acgaccactg aaaaattaat
    15361 ccaaagcgta gaaaacatcg gttatggtgc aattttgtac gatgaagccc acaaacaaaa
    15421 aattgcagaa gaaaagcaag cgtatttgaa aaaaatgcta ttcgatctta tattgagtac
    15481 ggttttgact ttgccgttga tgttgtcaat gattgctatg atgttaggaa gccatgcggc
    15541 gattgttcac ttttttcatt ttccaatcgt ccagttagtg ctgtctgctc ctgtccaatt
    15601 ttatgtcggt gctcgttttt ataaaggtgc gtaccatgca atcaagacga aagcaccaaa
    15661 tatggatgta ttggtagcaa ttggaacatc ggctgccttt gcattgagca tttacaacgg
    15721 atttttccgt ggacaccccc aagatcttta ttttgaaagc agcagtatga tcatcacgct
    15781 gattttgctc ggcaaatatt tggaacatac agcaaaaacg aaaacaggta atgcaatcaa
    15841 acaattgatg tctcttcaaa caaaaaccgc ccaagtgata agaaacggga aagaagaaac
    15901 gttagcgatt gaagaagtag tcgttggtga tcaactagtc atccgtcctg gtgagcagat
    15961 tccagcagat ggtcgaatta tttcaggaag cagtgcgata gatgaaagta tgcttactgg
    16021 tgaaaatctt cctgtagaaa agaatccgga tgacacgctt tttggcggga caatcaatac
    16081 gaatggtctg ctacacatgg aagtcacaca agtcggcaag cagacagtat tagcgcaaat
    16141 cattcaaatg gtggaagatg ctcaagggag caaagcccca atccaaaaaa tcgcagatcg
    16201 gatctcaggg atttttgttc cgatcgtatt agtcatcgcg tttatcacgc tcatagctac
    16261 aggtttgatc actggtgatt ggcaactcgc attgatccat agtgtctctg tattagtcat
    16321 tgcgtgtcca tgtgcattag gtttagcgac cccaacagct attatggtag gaacgggtgt
    16381 tggtgcacga aatggaattt taattaaagg cggagaagca ttagaagcag ctgctcactt
    16441 agacagcatt gttttggata aaactggaac gattacagaa ggaaagccca aagttacaga
    16501 tctagtcggt tcaaaagaag tcctatctat tttttatact ttagaacaag cttctgaaca
    16561 tccactaggt aaagcaatcg tggaatacgg aaaactgcaa gaagcagcaa catacgatat
    16621 gattgatttt acagcccacc caggtgctgg gatcagcggt acgattaatg gagtacgcta
    16681 ttttgcgggt acacgtaaac gattaattga attgaaccta tcatttgatg aataccaaga
    16741 acacgcgtta gaattagaac agcagggaaa aacagtcatg tttttagctg atgaaaagca
    16801 agtgatcggg tggattgccg tggctgatca aatcaaacta gaagccaaac aagcaatcaa
    16861 gcagctccag aataaaggat tagatgtttt tatgctgaca ggagacaaca aactggcagc
    16921 cgaaacgatt ggtaaacaag ttggtattga tccaaagcat atctttgctg aagtacttcc
    16981 agaggataaa gcagcctacg tagaaaaact tcagaaagat ggcaaaaaag tcggtatggc
    17041 aggcgatgga atcaatgatg caccggcctt agcattagct gatgtcggta tggcaatggg
    17101 aagtggaacg gacattgcga tggaaacagc cgatgtgacg ctgatgaata gcagtttggc
    17161 atccattgca caaacaatcg agttgtctag gttgacattg agaaaaatca aacaaaatct
    17221 tttctgggct tttgtctata atacgattgg tattcctttt gccgcactag gctttttgaa
    17281 tccaatcatt gcaggaggag cgatggcgtt tagttccgtc agtgtattgt tgaactccct
    17341 gagcttaaat cgacacatga aaaagtaaga gaagggatgg agtaaaaatg aatgggaacg
    17401 atatggagaa aaaacatgag cataaaaaag aagtagtaaa caaagaaagt acagaatctc
    17461 atgaaaaaca gatggagatg aaacatgacc atagtcagat ggatcattcc atgcacatgg
    17521 gacataacca tggagatata gcaaacagca aacaaatgga ccattcgatg cacatggatc
    17581 acgaacacgg cggcatggat cattccatgc acatggggaa tttcaaacaa aaattttggc
    17641 tatcgcttat cctagctatc ccaatcattc ttttttcacc aatgatggga atggaatttc
    17701 catttcaagt aacgtttcct ggttctgatt ggctcgtatt gatcttagca actatactat
    17761 ttatctatgg cggtcagcca tttttaagtg gagctaagat ggaattgaaa caaaaaagtc
    17821 ctgctatgat gacactgatc gcgatgggga ttacagttgc atatatttat agtgtctatt
    17881 cgtttatcgc aaatctgctc aatccacata cacacgtcat ggatttcttc tgggaattgg
    17941 ctactttgat cgttattatg ctactaggtc actggattga aatgaatgct gtgtcgaatg
    18001 ccagcaatgc tttacaaaaa ctagcagaat tactgccaga atcagtgaaa cgcttgacga
    18061 aagatggaaa agaagagaca gtttcattga aagaagtaaa cgaaggtgat cgtttgatcg
    18121 tccggtctgg tgataaaatg ccaacagatg gtgtaatctt gaaaggtgaa acaattgttg
    18181 acgaatcagc tgtgactggc gaatcaagag gaatcaaaaa acaagccaat gacaaagtga
    18241 ttggcggttc tatcaatggg gatggaacga tcgaaatcga agtaactgga actggtgaaa
    18301 atggttatct agcgaaggtt atggaaatgg tgcgaaaagc acaaggagaa aaatccaagc
    18361 tagagtcatt atctgacaaa gtggcaaaat ggctgtttta tgtcgcatta attgtgggta
    18421 tcctggcatt catcgcctgg ttgttcttga cagatcttcc taatgcatta gaacgtatgg
    18481 taacagtctt tatcatcgcc tgtcctcatg ctctaggttt agctatccca ttagttgttg
    18541 ctcgctccac atcaatagca gctaaaaacg gattactttt gaaaaaccgc aatgcactgg
    18601 agcaagccaa tgatctagat gtgatcatgc tggacaaaac aggaacacta acagaaggaa
    18661 aatttactgt aacgggtgtc gaagtacttg atgatgcgtt caataaaaat gaaatccttc
    18721 aatatctagg agccttggaa gcgaatgcca atcatccatt ggctgttggc attatgaatt
    18781 atttgaaaga acatgaaatc aagccttatc aagctgaaaa tctaaaaaat ctttcaggag
    18841 tcgggctaga ggcaacagtc aaaaaacaac aagtgaaaat agttaatgaa aaagaagttg
    18901 agcgtctcgg attaaacgta gaacaagcat tattgaaacc ttaccaagaa caaggaaata
    18961 ccatcagctt cctgatttta gaaaatcatc tagctgcaat cgttgcttta ggtgacgtag
    19021 taaaaacaga agcaaaagaa tttatccgaa cattgaaaga aagaaaaatc actcctgtca
    19081 tgcttactgg tgacaataaa aacgctgccc aagcagtagc agattattta gggatcgaag
    19141 aatattatgg cggtttatta ccagatgata aagaagctgt tgtccagaaa taccttgacc
    19201 aagggaaaaa agtaatcatg gtgggtgatg ggatcaatga tgcgccaagt ttagcccgtg
    19261 cttcaatcgg gatggcaatc ggtgcaggga cagatatcgc aatcgattct gcggatgtcg
    19321 tattgacaaa cagcgatcca aaagatattt tgcattttct tgatctagcc aagcaaacgc
    19381 gaaagaaaat gatccagaac ctctggtggg gggcaggtta caatattctt gcaattccgc
    19441 tagcagccgg aattcttgcg ccaattggta tcatattaag tccagcagtt ggtgctgtct
    19501 tgatgtcgtt aagtacggta gttgtagctc taaatgcttt gacattgaaa atcaaataat
    19561 aatgaaaaaa ggggctcttt gtcaataagg actgatgggt tgtacaaaat caaatctggg
    19621 tcagaatgaa cctcattctg gctcagattt tttgttatct tactttgatt aattgattga
    19681 tcaaaaagat tctaattctc atgttctcaa atgatttaaa tccgtaggat actc
//
LOCUS       DACDIP010000049        19215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDIP010000049 DACDIP010000000
VERSION     DACDIP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.049"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..452)
                     /locus_tag="IV257_001963"
     CDS             complement(138..452)
                     /locus_tag="IV257_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815067.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            738..977
                     /locus_tag="IV257_001964"
     CDS             738..977
                     /locus_tag="IV257_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815068.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1339..1599)
                     /locus_tag="IV257_001965"
     CDS             complement(1339..1599)
                     /locus_tag="IV257_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815069.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1784..2281)
                     /locus_tag="IV257_001966"
     CDS             complement(1784..2281)
                     /locus_tag="IV257_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8815070.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2411..3121)
                     /gene="budA"
                     /locus_tag="IV257_001967"
     CDS             complement(2411..3121)
                     /gene="budA"
                     /locus_tag="IV257_001967"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8815071.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3134..4798)
                     /gene="alsS"
                     /locus_tag="IV257_001968"
     CDS             complement(3134..4798)
                     /gene="alsS"
                     /locus_tag="IV257_001968"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8815072.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(5003..5665)
                     /locus_tag="IV257_001969"
     CDS             complement(5003..5665)
                     /locus_tag="IV257_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8815073.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5675..6490)
                     /locus_tag="IV257_001970"
     CDS             complement(5675..6490)
                     /locus_tag="IV257_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8815074.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6752..7195)
                     /locus_tag="IV257_001971"
     CDS             complement(6752..7195)
                     /locus_tag="IV257_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8815075.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7329..7667)
                     /locus_tag="IV257_001972"
     CDS             complement(7329..7667)
                     /locus_tag="IV257_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8815076.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7655..8032)
                     /locus_tag="IV257_001973"
     CDS             complement(7655..8032)
                     /locus_tag="IV257_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8815077.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            8256..9449
                     /locus_tag="IV257_001974"
     CDS             8256..9449
                     /locus_tag="IV257_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8815078.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9615..10037)
                     /locus_tag="IV257_001975"
     CDS             complement(9615..10037)
                     /locus_tag="IV257_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8815079.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10627..11082
                     /locus_tag="IV257_001976"
     CDS             10627..11082
                     /locus_tag="IV257_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815080.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(11244..12416)
                     /locus_tag="IV257_001977"
     CDS             complement(11244..12416)
                     /locus_tag="IV257_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8815081.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12622..14051)
                     /locus_tag="IV257_001978"
                     /pseudo
     CDS             complement(12622..14051)
                     /locus_tag="IV257_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(14048..15550)
                     /locus_tag="IV257_001979"
                     /pseudo
     CDS             complement(14048..15550)
                     /locus_tag="IV257_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(15547..17874)
                     /locus_tag="IV257_001980"
     CDS             complement(15547..17874)
                     /locus_tag="IV257_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8815082.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18717..19010)
                     /locus_tag="IV257_001981"
     CDS             complement(18717..19010)
                     /locus_tag="IV257_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8815083.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5625 a         3739 c         3280 g         6571 t
ORIGIN      
        1 tctagagaaa ttaattcatc ctaacataaa ataattgttc actcaaaagt ttttctagaa
       61 caattaaaac agcaggcttc acattcgcta tacatttgta agaagtaata attcctgtac
      121 taaaaggcat aattgcttta attatttttt gacttctctt caaacacaag aagtagttgt
      181 gtaataattg cagaaataag gattccaata atagatataa aatttgacat ggaaacgtct
      241 ataacattaa aagaataaag tgttgaagaa agtaaactga ttgaaataac ccctccgtaa
      301 aaaatagtct gtgaatatac ccactttttt tcagaagacg tacttcgttt ggttctaaaa
      361 cctatccctg catttctact cttatcaaaa aaccaaatat ttagaagtaa taatattgct
      421 attattaaga taaaattact gtaaaaatcc aaaagctatc ctcctagcaa ctatttaaat
      481 acccaaaaaa attacctcta gataatatca tacctacgtt ttcacttata aattttttca
      541 taaaaaaagc gtttagaggc ataatcgtta atacagatgg taaccaatgt atctggtttg
      601 aactgggata tcaccctctc catatatgta ttactaagac taatagatac tataaaagac
      661 ttgatagttt tgcattgatt ttgcctaata ctgagcatag gtgtatgcta aacgatacta
      721 taaggagtgt gtacataatg aaaagaattc tgtttatcag tttatcaaat ttattgttac
      781 ttactgcttg tgggacttct gcatcaaaga acataccttc agatacgcaa atttcgaata
      841 gtatcgattc aacaatttct acaaatgaga aaaattctcc ggtagaaact tccgatataa
      901 aagtttctac ctctgcaaaa aacagggtaa agaaactgtc agcacgtctc ctttatttca
      961 aacaaactct gaattagctt cttcatcttc ttcagcaaat aatgatgata gtttcgcgtt
     1021 tactcctaac taaatgcaac ctttacaaat gctttacgat aacaagcctg agctaaatga
     1081 tcctgatatc agttttgcat tttttgaaat gattggatcg gatgatcttt ttaaaactac
     1141 tagtttgtct atccgaaaat aggacagttc tggcagtata ggtttttatc gagtttctcc
     1201 accaggcctc gttacagaga cagattctct aggcgaacct tttgaataaa tcaaaaatca
     1261 tgaacaatca ctgccaacta aataaagtgc cttcaaaagc ttggtcttct cgcttttgaa
     1321 ggcactttat ttaaatcttt atgaaactaa agtatgttca tatttgttgc tatagttata
     1381 tagatattct gttgtttcca actcataaac gtgagtatat tttgccacta cttttccgca
     1441 tttttctaat atttcattgc gatcgacgtc atgatattcg tctacgcaga tcacaacaca
     1501 attttctaat ttactgatta cttctccaat cactttttgt ttcaagccaa taggttggca
     1561 aatatatttt tttcctaaaa tgatagtttc gttattcata tttcatccat ccttaatgtt
     1621 ttttcattat ttaaaatgat aataacatta aaatatgatt atgaaaatca ataacacttg
     1681 tgattttata aaaaaataaa aaaaccgaaa aacagaaatg cccactgtct ttccggctct
     1741 ttttattctt gatttctctt cttttttgtt tcaatagacg attttagtta ttaataaatg
     1801 aaaaagcatt tatattaaaa tttgctaata aaagatctac atatttttct agaaatgctt
     1861 gatcgatttc atcataccct ttcgttactc cgcaatcgat atctaaaaca ccgattaaat
     1921 taccttcttt taccattggc actacgattt ctgattgtgc ggctgaatcg caggaaatgt
     1981 aatttgcatg ggttttgaca tcatcaacga tcaatgtttc ttgtttttca gctgattctc
     2041 cacaaactcc ttttcccatc ttgatacgtg tgcaagaaac tcgcccttgg aacggtccta
     2101 aaatcaattc attaccatcg aataaataat atcctgcaaa gacagtctca ggcaaagtct
     2161 cattcaacaa tgcagatgaa tttgctaaat ttgctaacca gttatcttct gcttctaata
     2221 atccttgttg ctgtaataat aaaatttcgt atgcatcttt tttctgtttt tctgtccaca
     2281 tgataaaaac cgctcctttt tgatctatgg agaagattat agctcagttt ttttgtaaac
     2341 gctaggtttt ctcctcaaaa aaacgagata cgtcaggtta atcaccttga acgcatctcg
     2401 ctgtcttttt ctattctgct tgttggattt ctgctgctaa atcagcataa tcaatcgttt
     2461 cgtgaacaaa agtcggatca tcaactggaa aatgctgtac aagggacgag atgcggctaa
     2521 tctcaacttt tccttcagcc acttcaaaat ccattacatg tccgccaaat gtatgtgtat
     2581 catcgataaa atgcaggtga aatcccgctg cagcaactcc ttggaataat tcaggcgtaa
     2641 agaacccgac taatgttcct tggacattgc tttgttggaa ttctggttga gactctgaaa
     2701 tttttgctag tcttctgtaa ggtttttctt gcttcggcat gatacggatg tgcatatttt
     2761 tgaataatcc agttatcttc actgccaaaa ataggttttc cccggcttct cttactaata
     2821 tgtctttttt caattgttct ccatctgcag tttttgagac agaaaaacta gtatctggcg
     2881 taaaatctgt aattgctgca tagggggtaa gttcacttcc atctaattgg gcaactgagc
     2941 catctgatct tccttgataa gctacgccat ctaaaaagat gacttcacca tcaagcccat
     3001 gcaatgttcc caatcccaga gttccttttt ctaatatatg agcaatcgtt tctgttccat
     3061 ccatcaagcc agccatcaaa gctcctaatg tcccgtgctg gtataagatt ttttcagtca
     3121 tgtcgacact tccttaatag aattgatctg gtaagattgt ttctcccaat ttctcattat
     3181 cacggtaatc aatcggaata tcaataatta cgggtccttc tgtcgcaaat ccttcttcta
     3241 aagctttttc taattcctct acacttgtag ctcggattcc tttagcgcca aaagcttctg
     3301 catatttcac aaaatcaact ggaccaaagt ctacacctga cgcccgctga tatttcattt
     3361 tttcttggaa ctctaccata ttgtagtgtc catcattcca aatcaaatga acgatattca
     3421 attttttacg taccgccgtt tctaaatcct gggcagagaa taaaaatccc ccatcgccgg
     3481 aaactgatac gatttgtgta tttggacgca ccaatgcagc agaaatcgcc catggcaatg
     3541 caacaccaag cgtttgcata ccattactga ataacaaatg acgaggttca taacttctaa
     3601 agtggcgtgc catccaaata taatgactac ctacatctac ggtaacagtc atatcatctg
     3661 ttacttttga ctgaagcgtg ttaataactt ctaaaggatg caagatacct gcttctcctt
     3721 ttgattgaac aatgtcccgc tccgttaatt ctgcttgaag agatgctaaa tattcttttg
     3781 catcttctga cacttgttga ggctctaatg aacctgtcaa taaatcaaga gtggctgaga
     3841 tatcaccgat caattctctt tcaggctgca taaaaggatc gatttcagct ggcgcttcat
     3901 cgatcacaat gatccgcgca tctttttccg cattccagtt tcttgcttca tattcgattg
     3961 gatcatagcc aatagcaata actagatcac ttcgtttcaa aagcatatct cctggttggt
     4021 ttctaaataa acccacacga ccaaagaaat gcgcttctaa ttcacgtgag atcactccag
     4081 ctgcttggaa tgtctctaca acaggcaatg ctgtctttcc aactaattgc ctgatggcta
     4141 acgtttcttt ttcacttgat cctcgcatac ctactaatag taccggtagt tttgcttctc
     4201 taatgcgttc agctaggtaa cggatatcat ctgcagaagc agaacctaat ttaggatcgc
     4261 tcaatggctt gatgacattc ccttggacag gtgcatccac tacatcttgc gggatactga
     4321 taaagctcgc gccttttttt gaactcttag ccatacgata agcattggca atcacttctg
     4381 aaattgtttc tggatcttgg atttctgcac tgtattttgt gattggctgg aacagtgctg
     4441 cattatccat gctttgatgg gtcaacttca atagatcgct tcgttttact tgtccggcaa
     4501 ttgctaaaac tgggtcacct tctgctgtcg ctgttaccaa acctgtagct aagttactag
     4561 caccaggacc acttgttgcg ataactactc ctggctctcc tgtaatacgt ccgattgctt
     4621 gtgccataaa tgcagcattt tgttcgtgac gagtcacgat taattcagga ccttgatcct
     4681 ctaattcatt aaatacacca tcgatttttg cacctggtat accgaataca taatcaacat
     4741 gatgattaat caaactttct actacagatt tcgaaccttg tttcattttt tcgtccattg
     4801 aacatcgtcc tttttatgct ttatttttaa ttattagtta gaaataatca caaataattg
     4861 atacagtttc taaacctgtt tcattcaaaa gtatagcacg ttttatttca tttgtttagc
     4921 catatgtttc aaaaaatgaa aaagaatgac agtttttaca taaaaactgt cattcttaat
     4981 tgaaagtttc aatttataaa gtctacagat ccatagttct catcaattta cgattgttga
     5041 agtaaatcaa accaaatacg atagaaggac ctagtagaat acaccattgg ggctttacga
     5101 aaaagaagag aaacaaactt gtcaatagta aaaaatctag taaactcaca taccattttt
     5161 tcaacgtata gtagaacgct atccgtagaa cttgatttgg tttcgacgct ggatttctca
     5221 cctgaaaata ccagtagttg atgctaaagg aaactgataa acaagcaagt aaaacaaaca
     5281 acggaatcaa ccattttcca taagaaaaac gtataaaaaa gaaacaatca aaaatcatta
     5341 tcacactaac tatccaagca aatagccaat aaaaaaatcc ctttagccaa aattggcgaa
     5401 acgtctttat gaacaaacgt actggtgaaa aacttttttc ttttgctaat tcttctgtca
     5461 tacctatcat cacgatgcaa cctggaccaa taaaaagtaa agaaaaaata aaggaaaaga
     5521 cattccttcc atctatcgac aaaaggaacg cagctgctat atacaagata ttgacacacc
     5581 aaaaacaaag gctacttgcc aatgattgat acaaccaata aaagaattcc atataaacat
     5641 ttttgtcaaa acgctgctct ttcataatca cctcctattc cttcattcct gatgaagcga
     5701 tcccttctac aaaatatttt tgcatcgcta aaaatataaa agcaacagga agaacagaaa
     5761 caactgaggc agccatgatt aatgcgtact ctgcatcaaa tagcccaata aacattttca
     5821 accctaactg gattgtttta ttttccgtag aagacaaata aatgaatggc cccatataat
     5881 cgttccatgt attaacgaat gtgatgatcg tcaaactggc aatcgccggt tttgtaagag
     5941 gtaaaatgat cttgcgatag attccccact ctgataagcc atcgatccgc gcactctcac
     6001 ataaagaatc tgggatacta ctgtagtatt gcttcaacaa gaacacacca aaagcattga
     6061 acgcttgtaa tagcaccaac gaccacaacg tatatgttaa acccactttt ctcatcataa
     6121 tgaactgcgg gatcatataa gactgccaag ggacagcaat cgtgccgata tatgctaaaa
     6181 ataaaatatc gcgcccttta aaatttagtt tagaaaaccc gtaagcagca aaactagaag
     6241 taaacaactg aatcaacgtg atcacaacgc ttaaaaaagc tgtattctta aaaaatgtca
     6301 ctaaaggaat acgagaccaa ataaccagat aattctctaa atgccaagtt ttagggatcc
     6361 attgaatcgg aatcgtgaac acttcattat ttgctttgaa tgaagcagaa accatccata
     6421 aaaaaggaat gatcgtcaat aaagctaata caatcagcag aatcataccc aaccattgaa
     6481 taaagggcat gttttttgaa cgaatttttg ctttttttct ttcatttcga acacctccaa
     6541 agttataatc ttcatttctt tgtaatgatt acaatccaga acaacggtta ttatagctga
     6601 ttcatttccg ttcttcttat gtttgtaagc gcttatttaa caagttatgt aaatctcttt
     6661 attttttttg atttaaaaat gaaaaaatac aaaagcaaga ctgtcatacg tctcgctttt
     6721 gtattgaaat ttcttcgatt agagatactt attactgttc aaaagcttct ttgaaacggt
     6781 gaatcaattc attgactaca ggtgataaaa tccgattctt tttccagact agcatgcagt
     6841 tcgtttcaat cattgggtca agcggaacga atgtcactcg ctttgaaaga tgacttacag
     6901 caccttctat gactaaagca gcacctacct gattctcaat tagcgcaaat acattgaaaa
     6961 tcaaattgta cgttcctgtg atcagcaact gctccttttt tatgcctaac cagtcagcaa
     7021 acattcgctg tatttctgga cgctttgaga tcaaaagagg tacattcgca aagtcttctt
     7081 ttgttatcac gtcttttttt gctaaaacag aattttctaa aatgagcaat ccccattttt
     7141 cctttcttgg taacaaaatc gtttcgtatt tctctgtatc tactggatct agcaaaatcg
     7201 ctaaatctaa caggcttttt tctagtcact ccgtgatatc atcacttgtt ccgctgtgga
     7261 tatgaaattg aacgctggga taatcacgga tcaacttttc tagtaatagt gccaatgtat
     7321 tcgaattatc agcttccaca cagccgatcg taaatcggcc gctgaataat tgtttttttc
     7381 tgttctcgaa ttcttgagtg gtttgattcg ttaaatctaa aatttcttct gctcggcttt
     7441 tttagaaaat aacctgcttc tgtcaagatc attttgcggt tttcgcgtac aaacagttct
     7501 gttcctagtt cctcttctaa ctccttcagt tgtcggctta aggttggttg tgtaagatgt
     7561 agaacttctg cggcttgaga gattgttcct tcttctgcaa ttgtccaaaa atattttagt
     7621 aagcgtaact ccatagcgat gctccttgat ttttttactt gatacattcc attttatttc
     7681 tagataacac acgatcatat ttcttgattt ttccttgtaa acgttccaaa cattcttgca
     7741 tttcagcaat ttttttcgtc aaaagttcac tttcttcaat caaaagctta cgccgctcaa
     7801 atacagttgc ttctccttcg ctagataggc gaatatattc aatcaaagac tcgatcgata
     7861 atccggcatt tcgcatacag atcgtaaagt cgacccattt taggtcttct tctgtataat
     7921 cacggattcc gccttttgtc cgattaactg gtggaatcaa cccgattcgt tcataatagc
     7981 gcaatgtatc ggccgaaatg cccttttctt cactgacttg tttgatattc atctgatcac
     8041 tctttctctt aatttcttgc cactttactt atgtatttta tttattctac aactttaagt
     8101 gtactccaag tcaatactca tttctattaa aactaagctg gattatctat atcggttgtt
     8161 tgtgtattat ttcaaataaa aaagtcttgt ttttttattc ttttttggat atacttttaa
     8221 caatttaggg aaattttagg aaaaggggta taaaaatgac aacgtatcag cgaaagttat
     8281 tattatcgac atcggcggga attgctttag agaatatgga tattatgttt ttagcttttt
     8341 cattatcttc aatgattact tcttttcata ttagtggtac gcaagcgggt ttcatttcaa
     8401 cgatcactaa tttggggatg ctcattggag gtattttttt tggcatcata gctgataaat
     8461 atggacgtgt gaaagtattt tctcaaacgg tccttctgtt ttcgattgct tctttattga
     8521 tgtattttgc ttcaaacatc tacttggttt atctctttcg ctttatcgca ggtgtaggag
     8581 cgggaggaga atacggggcg tgtatgtcct tgatttccga aagtttttca aaaaaacaaa
     8641 ttggacgagc ttcttcggtt gctggtattg gtgcgcaggt cggtgcggct ttggctgcga
     8701 ttttagctgc tgtgatcatt ccaacgtttg gttggaagat gttgtatgtc gtaggtgtac
     8761 tgccggtgtt gatggtcttg ttcattcgtc gcggcttgac tgaaccaaag gaattcaaat
     8821 ctgtacaatc tgccgaaaag aaaaccaaac ttagcagact gtttgaatca aaacatcttg
     8881 cttggcaaac atgtggcctt agtatcatgg taactgtcca aattgccggc tattttgggt
     8941 taatgaattg gctgccttca attatgcaaa atcgattagg gctttcggtt tctgggtctt
     9001 cattgtggat ggtcagtact attttaggca tgtctttggg gatgatgact tttggcataa
     9061 tcatggataa attggggcct aaactagcct tttctttgtt tttaatttgt tcatccttat
     9121 ctgtctatct acttgtgtta gcccatagcc agtggagttt ggtagcagcc gctgtgatag
     9181 tcggctactt tatcaatggc atgtatggtg gatatggcgc agtgattagt agtttgtatc
     9241 caacagaaat ccgcgcgacg gcgaataatt tcattatgaa ccttggacga gctgttggtg
     9301 gtttttcttc tatcgtcatc ggtttcttga tggatcatta tagtttaact gccgtaatct
     9361 tgtttttgag cggtatttac ctagtcagtc tgctggtctt gacaactctt actggtgtta
     9421 cagcattaag ggaaagcttg cagcattgat agttgagtct gtaaatgaca agaaagaggt
     9481 gtggcaaaag cttgcatctg cggtaataag accagccaaa tcaaaaacat gagacactgt
     9541 ttccgtttgg ctggtcgtat tacttgatgc agggcagctt tttcaactgc taatttcctt
     9601 ctatctggcg gctactattc tttgacgtcc gtcatttcaa agtactttac taataactta
     9661 gcataacgaa aaactgtgga tacactcgta tattgagcgt ctgcataatc ttccaaattt
     9721 ttctcaggat gttgatacat gaattcaatc aaatgataat tatatgattt tctgtagaaa
     9781 tgaagataaa taatgtagat tgggaagttc tcctgctttt ccatagaaaa gcgccgatta
     9841 ttctcattct tcaaaatgac cttatcagga tatttttgga acaattcttc taaatctgtt
     9901 agataattca agatagactt acttgatacc ttcaattcgt tttgtagttc ttctattgaa
     9961 caccaacgtt ctgtttcaag cagtaaatga atcattttaa ttttcttttg agaaactttt
    10021 gttaaaaaag tctgcatatt ttctcctcct gtatctactt tactatacta catacaagaa
    10081 aaaaaacgga tacaaatatc atattgtcac accgtcaata gtgcataaac ctgttattat
    10141 ttctcttttt ttcctaagta ttctattgat atgaatcatt taatcgttta ttatataaat
    10201 gtcaataatc taataataaa ataacgtaca tattttatta ttgcatactt aataacagaa
    10261 ctatagcaaa aaatcccgtg tatcaaaacg ggattagatt aagtttcctc aatcactatt
    10321 cataacacaa ttattataac aagtaagtaa acaattgaaa aggacattta ttgaaaacgg
    10381 ttgtttttta cttaaggagg aaatttagtg aaaaaattag ttgcattgtt tttggtcgca
    10441 ggaagtttgt tgagtgggtg tggtgctact acgtctacta ctcaaagcac aagttcagct
    10501 caggcttcca gcagcagtac cacgatctcc gaaagtacgg cagattcatc gaaagaacaa
    10561 gaacaaattg aaaagaaaaa agaagaagag aaacaaaaag cggaaaaagc tgcactagaa
    10621 aaaaaagtga cagaagcgga atctgctttg tcacaagctg aagcaaatcc tacaaaagaa
    10681 aactatgcaa ctgcttcagc agctatccaa gcgctacctg aaaacaaaca agagttgaca
    10741 agccgtcttg ctgcaattga ttccacaatc aaagcaaaag aagcagaaga agaacggaaa
    10801 aagcaagaag aaattgcacg acaacaagca gaacaagcgc aacaagaagc aatcgcacag
    10861 caacaagcag aacaggcgcg acagcaagca gcagctgaac aggcctctca gcaggcacaa
    10921 gccgcagctc ctgagcaaaa taatgaacaa actgtctatg tcacaccgac cggttcaaaa
    10981 taccatacac ataaatgtgg aaatggtacg tatacccctg ccactttaag cgaagctcaa
    11041 gctcgtggtt taaccccttg ctccaaatgt tatggaaact aattcaaaag ttatactata
    11101 ataataaaat tcttctgaaa cataaaaaaa tagaagctgg cgttccagat agtaaaccaa
    11161 aaatagcagt ttacacgtcc atttcgtcag cttctctctt ttttcattta gtctgttccc
    11221 tctgcttttt tggacatctt cattcattct ttctcaatca aatagtacac ctctttttct
    11281 tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa actgtttcga atgtttatgt
    11341 tttacatgga ttttaagcag gagctcctca tgttgcaagc gatagttcct gccttttaaa
    11401 tcagaaaatc tcactagtcc gtattcatca gaagctttca caaatggaac tttttctcta
    11461 gtaatatagt gtttgccgtt gagcgttttt acagaaaaga ccattccttc gattggtcgt
    11521 ccgtttgtgt tctttacata aaaagataaa ggatagtttc gcttagcgat ggccaaagaa
    11581 tctaaataag cgatgagtag ccaaactaag ccgattgatc caagtagtat accgataaac
    11641 caaataaaaa atttccattt tttccattga tcaattcctt ggatcgcttt ttttgcctct
    11701 tttgcttgat aaggaatacg atgccccgtc acaagcagtc gatgtgtatt gaccatataa
    11761 ggagtacagg taagcaaagt cacgagatct ctgccttttt ggatacccaa agaatctact
    11821 tcatcaggta agaccacttg gattttttct acttgatacg ccaatgtctt tccattgatc
    11881 tggatgaaaa actggtctcc ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct
    11941 gggagtccac gatgcccaga aagaacggca tgggtgtttt tcccgccagt cggataagaa
    12001 ctttcttcca ataatgacgt tcctttttgc aaaaatattt ccgttgtctg atcgaatata
    12061 ggtaagctga cattgatttt tgggatttcg atgactccaa gcatatgttc cataaaaaag
    12121 gcattactag gtaaatcttt taatgctttt gcatcaactg cttggttgaa actgcctatc
    12181 cctggtgata cgttttgctc tgccaactgt tggtttttct ttgtcatttt ttcctgctgt
    12241 ttttttattt cctctgaatt cttattcgat gcttgctttt gataatggat gatcatttgc
    12301 tgggcaagaa aatcattcag tgaatcttct acaaaaggat aagaaaagat cccgatccca
    12361 acgactaaaa tgattgccat gaagatgtcc agtattctta ttctgctttt attcatcatg
    12421 tccacctatt ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta
    12481 ttcgctcaac aaacggcaaa atagttcctc ctattttgcc gtttgttgag cgaataccaa
    12541 gagaccgacc acttgtcaca acctttctcc ccatttgaat cactatcgtt tatttatcgt
    12601 atgatttttg tttccaatag tttaagcttc tgtttcttga tggcgttttt tgaagtagat
    12661 cactgctcct ccaactaaag cagtacctac caagatgaat acgatgatcc ccataccacc
    12721 agttgaaggc aagattcctt taggttggtt atcgatcgtt gtttcatcag aagttgttgg
    12781 atcatcatct actgtaaatt cagtgtacgc agcttcattc ttaggtaatg cgtagccttc
    12841 tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc agcccttcga ttgaaaattt
    12901 cccttctgaa tcagaaatca atttcacttc ttcccagtca gaacctgtga cttcatctga
    12961 ccaagcatat gggtattctc ctgaggcaag atttgctatt tttttcatta atttaacagt
    13021 tggatctttt gctcttagaa tagaaaattc tgcatcggct aatttttctt gcgtgttcac
    13081 atcttgtttg atgaagtgga aatcaccgta gttttggaca ttttctacag taatcgtgtt
    13141 atccgtagtt gtattttccc gattgactac accgtttgga ttcccaagat tggcttcatc
    13201 tgcgccatgg atgataccgg ttgtttgttg caattttccg ttttttacta taaaagtagt
    13261 ttctttttgt ggattggtta attgataacc atctggtgct tttgtttcaa ctaaagtata
    13321 attgccgtct attaatccat tgacactgat ggatccttca gtatcagttg tgaatgtcaa
    13381 cgctgactca gcaggatcat agagagcgac accagtgttt tcatcaaaac cggtcacgaa
    13441 ttgattctct gaatttttga tctggaattg cgcccctgct aacttatcta cccctgaaaa
    13501 gttcccagct ttctggattt tgatattgct ttctttgatg acatttttag catataggtg
    13561 aatcgtgtct aaaggtttgt cagggtctga tgaattcggg ttataaaagg gtaagcttaa
    13621 cacgatattt ggcgacgttt cgacaccggg ctttggtgtt tctacaaaga cgtatacagc
    13681 atctttccca tcagatttac ctggaagagt caggttagct aatccttcac cagaagtcgt
    13741 tacacttgcg aagggtgtag cgtctgctgg aactgctgtg tctttacttg gatagtacgt
    13801 agcagtgatg tcatatacgt tgaattcaac gccgttcaac ggttcgctat tcggaaatgt
    13861 caattccgtt ccgttgttta tttgattttc tggtgctgaa gtgaatttca atttatgcaa
    13921 attaaccgta acattttctg gtgtaggtga tacggcctag aaacctctgc agttcctagg
    13981 ccgagaacga aaggaagcag catgatagca gttcctaaaa acgtccatat ttttgttttg
    14041 atgtttttca ttttatttcc ctcctattgt ttcctttttg ttgtttttta taaaaccaag
    14101 ctgtccctgt tgaaaggact agcaacaacc ctaaaattga gaaacctcca agcatctctt
    14161 cgcttgtttt tggaaagctt cctccatttt tttcatttga tggaccattt gtctgcatct
    14221 gaactggcgt ttttccatta ttcgtcggat ttgcttttgg ttcgttgatg atttccaatg
    14281 gtataggatg aagcggatca taactgcctt tttcaacttc aaagagaatg gttgattgac
    14341 tcaatatata tcctttaggc ggtttaatct ccactagttc gtaacttcca aaagacaatc
    14401 catggacaga aaatgttcca tctttactgg aagtaaaaat cgttgctctt gaatcatctt
    14461 tggctaccca atgatttatc ccgttttctt gcgctaagta ttggtcattt tttttgacta
    14521 gaaattgagc atctgctagt ttttgtttcg tttgccgatc cactttgaca aattgtttgc
    14581 ctcctgtttg gatggcaaga tgtgtttcta ctgtttgatt gtcagtcgtc aaacgtacat
    14641 cgttatcaaa tgtcgttttt tcactgtttt ctttcaatgt catttggtaa cggatcgtta
    14701 attttttccc agcaaacgaa ctaagatctt ttccagaatc tgataagaca aaaccatgtt
    14761 ttgttttttg tgtgtgaaat gtatgaatct tgtctccacc aatcaaaaaa tcgatcgttt
    14821 ctggttctag ccataactga ggatcagctg tatctttgat cgtataggtt cctatctcgt
    14881 tgatgttctc aggaattgtt acggtgatga gataaggaac tttatcccca tctgcaaaat
    14941 ctgtctgctt attctctact gtttttgtta atgtgagtgc agcaggtatt ttttgttcgc
    15001 tctttggata caagtggatg gtcgtcaatt tatgattaga tgaatctgtc acaggaagca
    15061 caacgacaaa tggtgctgat ttttctttta cttaatctga agtctttatt tccgcaaatc
    15121 ggtaaacagc gtcacgacct tgctcatctt tgtccgatgc actaaaagaa gcaatgccct
    15181 cttcctcgtc tactgttttt gtcacatttt ctgccagtat tttttcacta tcactttttt
    15241 gtgctagttt tctttgtgcg tcttccacac tacttccttc tgaccgcagc ttgtaaaatt
    15301 catccgagac atcatatata gcgaaagtaa cgccgttcaa cccttttact ccttgaaagt
    15361 tatccgggat atttattccg tcgttttgac tgatacttgg catcttatcg ctttcgaaca
    15421 ttcgattatg caaagtaaat gtaataggtg attctctagc tgacacagtc ggagaaccta
    15481 taaaaaagac cagtaggata aaaaagagaa cagcaaatat atccacacgt acattttttg
    15541 tttttttcat cgctgttcac ctgctttttt cttacgatgg ataaaataaa agaagccaga
    15601 aaaaataaag atcagtccta aaaagacaaa gggcagtaga cctatcccac ctgtcgaagg
    15661 aagcaagaga gtcggattga tcgtcaactt agcggtatct gttgcgataa tcgtataatt
    15721 attgtttaca cttccttcgc ctaaactaat cgttactatt tgtgatccaa tatctgtccg
    15781 gctgttaact gttagggtat aatccaatga ttcgcctaag acaacaccgg taacgtggag
    15841 atttccaacc gaaatcgagt gactttgggc atcataatta aaggcagcgt catcgacagt
    15901 aatcactgcg ttagctttat agatggtata agttcctgat gtgttggcta tatcagtaaa
    15961 tgacaacttg ggatttgctg ctttgacttt gtctattccg ctattcgtta gtttaattgt
    16021 atagctgcca gcattaacca tattttttgc tgattgtgca tctgctgtgt aatccatact
    16081 gctaagtgcc acttgtactg gagtaagcga actagtgaca ccctctgcta gcttgacatg
    16141 gattgacggg acacctgctt ttccatcata tgtcttactg ccactgagct ctaccgcacc
    16201 gctgatttgc ttaatcgtat agacatcaga tgtctgcacg atagtaaata aatagtttgt
    16261 tctggcagct agtttagcta gtccgctttt tgtcaattgc caagacgtat aggttccaat
    16321 ttcagtaggc gcagttactt caactccctt ttcattcatc gctttgaaat cagttggatt
    16381 ccatgtgatt tgatcaatac tgagatcatt gtttaaaagg gatgttgatt ctgttttatc
    16441 attaaaggca tatttaacga tcggtacaac agaaagacca tcatagtact tactgccatt
    16501 tgcattgatt gatactgttg catttgtctt ataatatagg atgacttcag aaatctgttt
    16561 tccatttgcg tcctttacga ttacatttcc atttgtatct aaactagcta aaacactcat
    16621 tgctgattgg ttctttgttt ctgtcgctga aacagctgta aaggtatatc cgttgatagt
    16681 aggcgtatta gtcgtcaggt cttgtgcagc acttccgtaa gtaagattca ttattttaga
    16741 aggagcaatc atctgtccaa aatagtcttg atacttaata gtgattggtg tttgtttgcc
    16801 tgtataggtc accgtatcga cgttactttc tagggctgtg aaagaaccgt ttgtttgtgc
    16861 aaaagaatag cccgttactg ctgttggagt gataaccact ttgtcaccca catggattcc
    16921 agataatgta gtcccgtacg ttccgatttt tgctgctcca ccattgactg tataagaaaa
    16981 agttcctgtc cgattcgcat tgacctgaac cgttgctgct tgataggttg gaaaatttga
    17041 aacatttccc ttaccatcaa cgatctgccc atttttgatc gagtaccccg tgaatggaat
    17101 atcatttgct aactgtatac cgattgaaaa agtactcata cctcctccgg caggaagccc
    17161 tccccatgtt gagggtgtaa aagaataact tttatccgca ttgggtgtaa ctgaatcgat
    17221 cgtatttcca gcggtagcca cccccatata gatacttttt gggaatgtga tagtagtacc
    17281 atttactgtg gagccaataa acagattatg gatatctgga cggttatcaa taaaagcgat
    17341 tgctttggtt acgccttgta acgcagtttt atccgcactc atcgatccgt taaccgcaac
    17401 aatgactcca tctttggcat atatcactgt atatccatct ggtatggtat gtccgttgaa
    17461 attcgcagtg gccgcaacag tactaaatgt ctggcttcta cctgtttctg aataagcttt
    17521 ctcccctgct gcttttgctg ctgtgatttc tttttctgtc ggatcaatca gttgagtcga
    17581 ggatgtggtt gaggttgtct cgttgttgct agactgattg gtgctttcta tggactgtat
    17641 ttcagaatag ggtggactat ttgtctcctc tgtcgtttgg ttagcaaaat ttgacgtttc
    17701 tgtataaacg tttacataaa tacttgataa gctccctaag aaagtaaaaa tcaagatgat
    17761 cgaaatcata atgggatgta agtattttct tttagataca gctttgagtc tagaaacaaa
    17821 atgtttttga catatacgtt ggattttatt ttgagttttc ttaatcattt tcatccctcc
    17881 tctcaataaa aaatcatcaa ataatccttt acacgttatt ctaaatttaa tttttattat
    17941 tttattgatg tatagaaagt ttggggcttt ttttctcttt ttattctaag gttattttag
    18001 tcttgtcctt taatacaaag attttatttt taaataaaaa atactctttt tttacgatta
    18061 ttgaaaaaaa aagataaaac ttgattaatt gaagtattgc ttaaatttgg aagctatgag
    18121 ggaagaataa aagagttagg acgggattat ctactatttt tctttgtttt aaatatttgt
    18181 tatctttgaa cataaaagag aaatgtgtcg actgcagtgt tgtgtccctc atcacgaata
    18241 aacggtgttc aattagctga tcttgtattc acttaaaaaa tggtaaagta cgagttgcta
    18301 ttccccattt tcaattttct aacgctcagg acaaaatact gttttcacaa cctcattgga
    18361 ataaaagcat atatattgtt tcaagaacga aatcttgtta tctttttaaa gggcatctgt
    18421 ccaaaatcta attttaggag gtacgttttg gtatttattc gtacaaataa aagagaatta
    18481 tctttattca tgtaacgcat ccaattgatt ttttcatgta cgctcctgga acgattttac
    18541 tgacgattgc ttatttaact gcgaatcgct cattagcttt tatcatagct atccatattt
    18601 tgaataacgt tcttggcttt gtattataga tacagatgac aaaaaaagtt caagtatgcg
    18661 gtgtagaacc aatatcgcat acttgaactt ttttcaatga gtataaatta aaacttctaa
    18721 ttttcaattc ccatcatctg atatgcccga ccgtctgaac ttcttttgaa aaagccaaat
    18781 gtgattagat aacgtctttg ttcgacataa tcgccactag aaaatacgtt cagcagtatc
    18841 gaattaacct ctgtttctgt ataagtcact ccatggtcaa agcgtttcaa gacttctttc
    18901 aataggatgt atttcttttt tgttgaactt ggtacttgtt ttacttttcc atcattcatg
    18961 aatgtgtcta aaactttcat cttttcttgc tccgtataag tataaaccat cttctttctc
    19021 cttttgattc taagaatact ccatttatgt tagaatattc tggatttttt attaaagcgg
    19081 ttactcttaa gaaagatatt atcatagtgc tctttttttt actactactt ataggtagat
    19141 ttattctaat ccattattct cccctaccaa taattaaaga aaaaactgga tcagggtgtt
    19201 caaattacat gctaa
//
LOCUS       DACDIP010000050        17589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDIP010000050 DACDIP010000000
VERSION     DACDIP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.050"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            32..997
                     /locus_tag="IV257_001982"
     CDS             32..997
                     /locus_tag="IV257_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8815084.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1096..2268)
                     /locus_tag="IV257_001983"
     CDS             complement(1096..2268)
                     /locus_tag="IV257_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8815085.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2255..3400)
                     /locus_tag="IV257_001984"
     CDS             complement(2255..3400)
                     /locus_tag="IV257_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8815086.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3414..4346)
                     /locus_tag="IV257_001985"
     CDS             complement(3414..4346)
                     /locus_tag="IV257_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815087.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4463..5542
                     /locus_tag="IV257_001986"
     CDS             4463..5542
                     /locus_tag="IV257_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8815088.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5539..6168
                     /locus_tag="IV257_001987"
     CDS             5539..6168
                     /locus_tag="IV257_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8815089.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6278..6505)
                     /locus_tag="IV257_001988"
     CDS             complement(6278..6505)
                     /locus_tag="IV257_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8815090.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6608..9196)
                     /locus_tag="IV257_001989"
     CDS             complement(6608..9196)
                     /locus_tag="IV257_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8815091.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9216..9875)
                     /locus_tag="IV257_001990"
     CDS             complement(9216..9875)
                     /locus_tag="IV257_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8815092.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(9924..10427)
                     /gene="trmL"
                     /locus_tag="IV257_001991"
     CDS             complement(9924..10427)
                     /gene="trmL"
                     /locus_tag="IV257_001991"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8815093.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10648..11514)
                     /locus_tag="IV257_001992"
     CDS             complement(10648..11514)
                     /locus_tag="IV257_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8815094.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11623..12264
                     /locus_tag="IV257_001993"
     CDS             11623..12264
                     /locus_tag="IV257_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8815095.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12326..13687)
                     /gene="mgtE"
                     /locus_tag="IV257_001994"
     CDS             complement(12326..13687)
                     /gene="mgtE"
                     /locus_tag="IV257_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8815096.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPLKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13705..14604)
                     /locus_tag="IV257_001995"
     CDS             complement(13705..14604)
                     /locus_tag="IV257_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8815097.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14606..15403)
                     /locus_tag="IV257_001996"
     CDS             complement(14606..15403)
                     /locus_tag="IV257_001996"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8815098.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15381..16085)
                     /locus_tag="IV257_001997"
     CDS             complement(15381..16085)
                     /locus_tag="IV257_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8815099.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16216..16791
                     /locus_tag="IV257_001998"
     CDS             16216..16791
                     /locus_tag="IV257_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8815100.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            16909..17586
                     /locus_tag="IV257_001999"
     CDS             16909..17586
                     /locus_tag="IV257_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8815101.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5198 a         3783 c         3133 g         5475 t
ORIGIN      
        1 atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa
       61 tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa
      121 aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat
      181 tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat
      241 cgatactttc ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga
      301 tactttctat cagatggaag tagaattccc agttgcttat attccagcag gttctggaaa
      361 tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt
      421 agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa
      481 aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac
      541 aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt
      601 attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg
      661 aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt
      721 tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt
      781 cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa
      841 aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc
      901 aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat
      961 cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct
     1021 acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct
     1081 ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt
     1141 acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt
     1201 gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt
     1261 attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa
     1321 gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt
     1381 gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat
     1441 gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga
     1501 ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta
     1561 actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg
     1621 gtttcgttta ctgatctctt tgcgattaaa cgctagattg atgacgctga aaccgctaaa
     1681 aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt
     1741 catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg
     1801 tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg
     1861 caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg
     1921 tatcttgatc gtcgccatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg
     1981 taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc
     2041 atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg
     2101 cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc
     2161 aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt
     2221 cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc
     2281 tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat
     2341 tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca
     2401 agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt
     2461 gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc
     2521 tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa
     2581 tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt
     2641 ctacttgata gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg
     2701 taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca
     2761 agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca
     2821 tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca
     2881 tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg
     2941 gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta
     3001 aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga
     3061 gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt
     3121 cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga
     3181 cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa
     3241 ttccagtatt aatcgtttca agattgccac cttgatcaat ctcgccaaag gccgccataa
     3301 acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca
     3361 gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct
     3421 ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct
     3481 atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta
     3541 tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct
     3601 attgttcgct gaatcgctga caacacattt tctgggagta atgggatgtc gatcgtcaaa
     3661 cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt
     3721 ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt
     3781 gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc
     3841 cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg
     3901 gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga
     3961 aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt
     4021 ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt
     4081 tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt
     4141 tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa
     4201 agcatacagt tgatggcagt cgatttccca ctgccgtttg gacctaacag acctaagact
     4261 tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg
     4321 actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct
     4381 gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt
     4441 tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct
     4501 actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta
     4561 tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg
     4621 gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct
     4681 gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt
     4741 aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt
     4801 aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga
     4861 gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca
     4921 aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg
     4981 gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt
     5041 acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa
     5101 tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca
     5161 tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc
     5221 tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt
     5281 aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt
     5341 tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag
     5401 cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga
     5461 acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt
     5521 accaagggag ggacaccaat gataacagta gtcattattg atgatgatcc atttgtgacg
     5581 aaatcgttac aaaccatttt agaaagcacg aaagagatcc gtgtgttagg cattggtcat
     5641 tgtgccaagg atgcgctagt cttatacgaa acccatcgtc ccgatgtttt gctgacggac
     5701 atccgcatgc ctaaacaaac gggcatcgat gctgccaaag aaattttggc taaatttccc
     5761 aaagccatcc ttttgctttt gacgacattc aaagatgaag aatacatccg cgaagcattt
     5821 tccatcggtg tcaaagggta tttaatcaaa cagaacttgc aagcgattat tccttctgtc
     5881 aaatccgctt ataatggaca ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa
     5941 atgatgaaga ataaaccgac gattgatagt catgcttatg cttcttttag tgaacgtgag
     6001 cttgcaatca tcaaagaagt agctgccggg aaaaacaata aagaaattgc agatgccttg
     6061 tacttgagtg acggcactgt tcgcaattac atcagccaat tactagaaaa gctagattta
     6121 cgggatcgca cgcaattggc tatctatttt tatcagcatt tgcaataagt cttatctaac
     6181 acaaaaaaac ctgaatgatg ctccttcgca tcattcaggt ttttttcaca tcttctattc
     6241 aattgtcccg caaaccattt cctcacgatt gaaagaatca aggattcgca gggctgccag
     6301 gattcgttgg attgttcggc gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt
     6361 ctagttttga ttcttcacga tcgtctcgtt ctacatcagg actttcattc aatttgaatt
     6421 ctttttccat ctcatctaac tttttaaagt cattgccaga ttcttttgct aatttcgcta
     6481 atttatcttc ggtttcttta gccataaagt aacgcccccc ttttgatttc agcttagcat
     6541 atcacactga aaaaacaaaa gaaaacgcta accaacgatc atttttagct atttctcatc
     6601 tttaaaatca agcatttttt gccggtataa aatcgtaggg agaactgttt tccataccaa
     6661 tcatcggatc tactaactgg ttatcgatca cttctgctgt aagaacgaaa tctttagaaa
     6721 cagcaggttc tacctcttct ttcttttctt ctccaacaaa tgaagaaaac agatctggtt
     6781 ccactgtttc cattacttgg tttttcgttt cattcgtccc actgtcatct tctacaggta
     6841 gcttttcttt tacaacccgt ggctcttttg tatagttccc atcttcaaat ggatcatttc
     6901 cgaccaaaga atcttcataa gctgctagtt tggtccggag agtgatattc attttatcat
     6961 tatccatgat ccttgatttc aacgtagtca tccggcttgc tgattcattc gctacacaag
     7021 tgacataggc ttgctcttcc cctaacaaaa tcaacagttc cttactgcga gtcactgccg
     7081 tgtagagcaa attacgctga agcatccgct gatattgatg gaccatgggc aaaataacca
     7141 tcttgaattc actgccttgt gatttatgga ttgagcaaca ataagaaaga gtgattttat
     7201 tccattcgtt tcttttataa gtgacctcat ttgcatcaaa ctggataact aattcatcca
     7261 ctttatcttc agaatctttt gccaagatga tacccgtgat ttctcccata tctccattga
     7321 atacattcaa ttcgggagta ttgactaatt ggaggacttt gtctcctatc cgataaacgg
     7381 tgtcgttcca atgtacttcc ttttttccag gtttccctgg attgaatatc tcctgcatca
     7441 ttttatttaa cgcatcgatc cctgcaggtc cccgatacat tggtgctaat acttggatat
     7501 cttgtgcacg gaatccctta tttttcgctt tttccacaat tttagagatc aagggttcaa
     7561 tttgataagc atcacttcgg aaataggaac gatctttttg attctttgtg aaatcttttg
     7621 gcagacgtcc ttctttgatc tcatgggcaa gaggaataat actagaacca tcaccttgcc
     7681 gataaatctg attgagttcc attttgggaa tttgttcgat ttccaaaaga tcatggagta
     7741 cttgaccagg accgacagag ggaagctgat ctttgtctcc tactaagata acttgcatat
     7801 tcgtcggaat cgctttgaat aatgtgttag ccagccatgt atcgaccata gacatctcat
     7861 caacaatcaa aagtccgcct tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc
     7921 cattcagccc cagaagacga tggatcgtac tggcaggcag acctgtcgtt tcgttcattc
     7981 gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt
     8041 ttgaaggatc caattccagt ccgttcaact ctgcaaaaag attgacgatt ccgttgatca
     8101 cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa cggattgctt
     8161 cagcgatagc ttgttcttgc gaaggtccat actgtatgcc gaatattttt tcgatacgcc
     8221 gaatattctt ctgcacttct ttttcaggat aatggatttc ttttttacga ctgagaaggc
     8281 gctggatcga attaccgatc ccccattcag aaaagtacaa actgttttcg taaagattag
     8341 tctcttcttg ttgtatcttt ccttcttcaa caagttggat aattacagta gcaacctgat
     8401 caggctggat ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg
     8461 tctccgccgg tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga
     8521 tagctgctcg aattcttctc ggtgaatctg ctgcaatacc gatttgttcg gctagattat
     8581 ctgcacgttt gaacccgatt ccttctatgt cttcgactag ctggtacgga ttttcttcga
     8641 tgatatccaa agactcgttc ttataggcct ggtagatact gaaagctaac tgactaccga
     8701 acccgtaacg attcagtccc atgatcactt gatccatccc gtggtttagc cgaatcgtct
     8761 cagcaagcat ttctcttttt ggcttcgtca aaccggagat ttgttttaat accgaaggat
     8821 cagcaatgat tttttcgata gcatcttctc ccagcaattc tacaattttt tctgctgttt
     8881 tcttcccgat tcctgggaat ttttcgctgg aaagatacgc tacgatccca tttgcagttg
     8941 ttggcttttc ttgctcatat ctttcgagtt taagctgcct tccgtattta ggatgatcca
     9001 ctaattctcc ataaaaacga taagtctcat tttcttgaat atcgccgaaa cttccggtga
     9061 ctacaatctc tttttcacgg taatcagctg tggtttcact gatgtgtacc aaaagcactt
     9121 tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca
     9181 tttcttctgg ctgcatcgga agccgcctcc ttcttctata gtaaaggttc tggtgctgtt
     9241 tcagttccta aaaaatctgt ttggttccat actttcagtt catatggcaa taatctgcaa
     9301 ggctcttcag tctctaaaat cgtcaaacta ctattgaaca atccacccat ttcacgcaac
     9361 tcacttagct cttttcctgt catccattga atggctgcag ttaaagaagc tccatgtcct
     9421 acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat ctgctacaac agtttcgata
     9481 cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag aaggatcata cagatctaaa
     9541 tgattacgca aattcggcag attatccgga tatttctggt acatttcatc gatcgactga
     9601 ccttctaaag tacccagccc tagttctctt agcttatctg tataaaaaat ctctaccgga
     9661 cgagcaagct ctttgttgat accttctgct gtatcacgtg ctcgctttgc tgtactggaa
     9721 taaattgctt taaaaggaac atattttacc tttttcccaa atgctttgat ttcttcgtaa
     9781 ctttgtggca aaagcggaga atcgccattg ctcccttgaa aacgtctttc ctgattccat
     9841 tgtgtcttcc catgtcgggt aaaatatagt tccatacgca gcaaactcct gtctttcttt
     9901 tattttactc tataataaac tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat
     9961 tctaaattag ggaatccttg ctggcgaagt gcttcataca ctatcattgc agctgtattc
    10021 gataaattca atgagcggac atgctcatca ttcataggga tgcgtaaaca cttttcttcg
    10081 ttttcacgca taaatgtttc tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc
    10141 tcttctccat cagcaaaatt ttcgtcacta tatactttat gggcaaattt tgagatcaaa
    10201 tgtagtttac gattacctaa aaatgttaaa aaagcttcta aattactgtg gtaagtaata
    10261 tttacatcat tccaataatc caaccctgcc cgttttagat gtttgtcatc agttgaaaaa
    10321 cctagtggct cgattaaatg aagcggagta ttcgtggctg cacacgtacg ggcaatattt
    10381 ccagtgtttg cagggattaa tggttcaaat aaaacgatat gattcatcag gatcattcct
    10441 ctcatattta tgttcgtttc tattttctga caatttgatt tttgcataaa aaaaaacgta
    10501 tcaactcggt gtcagtcact gcgagtcgtt acgttagtga agctcttctc actaactttt
    10561 atcagaacag gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc
    10621 tcaacttttt tttgcaacct ctactcatta aaaactgcta gaaaaacgtt ttctcttagt
    10681 tccttttaat aattctatgt caattgtaat cttcttcaaa ggattcttat ataagtctgc
    10741 tttgattcgc tcttctacct gccattctaa aggtgacatg gccaaaagat ctttttgatt
    10801 ttcctctggt atctcaaatt cataggttac acgctgacgt tcagttaatt tcattgcttc
    10861 agcaaacttg gctactacac gttcattgga ataactctgt ttttcaggtt gattcggata
    10921 aaaagcctga cgtagttcct ttaaataatt ttcttgtggc accaccttca acacttctcc
    10981 atcttctgag agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac
    11041 cgcgttgaat gtttgatcag caaacggcaa attggtcaaa tcagctacac accaaaatgc
    11101 ttcggcagat tgttctgtag ctgcgtagac tccttctttt gaaatatcaa atccaacagc
    11161 attttgaatc tttttcaatt ttgaaagttc attcaagaag ctaccttctc cgcagccaac
    11221 atctaatagc gagacttctt caggtaaatg ctgcgcaata gtctcaataa cgggctgata
    11281 catgccagat tggatcattc tacgccttgg aacgaacatt ccttgatcgt attctgtttt
    11341 aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt
    11401 tttgcatatc aagccattat cagacttttt tacttcttct tggcaaaccg gacagcggaa
    11461 cagctgatga ttttctgcca aaaactggcg tcctcggtca atttttttta acatagttcc
    11521 ccctcatttc tgttttatct taccgttaga tgctcacttt ggcaactgct aaaaatatga
    11581 tacaatgcct agaagaaagt gaaagataaa ggagaagatt agatgatcaa agaattttgc
    11641 gctgaaaact acacatctat tcctctagca attgctagag gagccgatcg gatcgaattg
    11701 tgtgacaact tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga agaaacgtta
    11761 agttatgctg gggaaaaaga tatccccgta atgacgatta ttcgtcctag agggggcgac
    11821 ttcgtctata acgatatcga attgaaaatc atgcatactg atttgattga agcaaagaaa
    11881 ttagggacgg atggcgtagt atttggttgt cttactcctt caggctggct agacgaagag
    11941 gccttagaac tattgatcga aacagcagaa gggttgcaga tcaccttcca tatggctttt
    12001 gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt
    12061 gaccgtatct tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg
    12121 cttaaagaat tgattgccta tacagatggc cgcttaatca ttttgcctgg tggtggtgtc
    12181 actgcagaga atgcagcaca tgtagctaaa gaattgaacg taagcgaagt acacggaacc
    12241 aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa acatttgcaa caggcataat
    12301 gcttattgca aatgtttttg tttttttaca taaaatagcc cataaataaa gaagcaatat
    12361 tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta
    12421 ctgctggatc aaatcctaga cgatccatta gcatcgggat taaactacct gcaagattcg
    12481 ccactgtgat tgcaaatagc atagccatcc ctatgacaaa ccccaagata aaattatgtt
    12541 tccaaaaacc tacgattaga aaaatcgtga tccccgtaac agctcctgtg acaacgccgg
    12601 tcaagacttc agccaaaaca agtcgaacaa atccatgatc tttctcatca gaaatcgcaa
    12661 gtcgacgaac agccacagct agcgactggg ttccagcatt accggcagtt cctgtaatca
    12721 gcgaaatgaa gactgctaaa atactcgctt cacttaccaa gtcttcatag tgactgatta
    12781 aagaagcggt cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg
    12841 ctgcctttag tggattttca ttaatttctt ccacattgac cccggcaagt cctgaataat
    12901 cgctagcagc ttcatcatca ataacgtcga tgatatcatc gactgtgacg attcctaaca
    12961 aatgatcatc ataatcggta acaggaagag ctaagaagtc ataatctcta aatgtttgtg
    13021 ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc atcagatcag
    13081 aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc cctactagct
    13141 gaccttcttg attaacgaca tagatgtaat agattgtttc agctacatct gcttcgttct
    13201 tcagcacgta catcgcagag cgaacagttt gattggctac gatcgaaaca aattcagttg
    13261 tcatgatcgc gccagccgtt tcatcctcgt aatggagaag ttccttgatt tcactggcat
    13321 cgtctgccga catcaaactt aaatatttag caatttgctt tttatccagc gtattaagca
    13381 agtcaacagc attatctgta tacatctcag aaagcatatc agctgcatag ctaggacgca
    13441 tttcatctag gtattctttc atatgttcgt tgtcttcttc aatcacatcg aacatatccg
    13501 ctaattcttt aggcgacaga taggaataga tcagctgcct gttctcttct gtaagagatt
    13561 ggtagaattg cccctgctca tagatatgca tctctaaaaa actgtttctg aatgcctgaa
    13621 tattattttc ccttaaatct tgagtcaact gggcaaatct ttcctctaat tcttgatttt
    13681 catccaaacc aatctcacct cgccttattc ttcaaaccgt ctcacaactg cctccatgtc
    13741 tgcggctaaa ggctgcttga actccagcaa ctcttctgta aatggatggt aaaaacgcag
    13801 atcataacaa tgaagggctt gtctttttat tcccagatcc attcgcccgc catacatatc
    13861 atcaccgagc agggcacagc caattgcgga aaaatgaact cttatttgat gtgtccttcc
    13921 tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt cgcttctcta tccagtactc
    13981 agtctgtgcc gaacgtcctt catcagagac cattcgcttc aatagcgaag aataatctcg
    14041 ggcgatcggc aaatcgatat gtccatgtgc cgataaatct gaaagccttc cactgactaa
    14101 ggcctgatat tttttcacaa acttcttttc acgaagttgc atatctaatt ttgcgtgcgc
    14161 aaatccatgc tttgcaaaaa gcatcaagcc agatgtatcc cgatctagtc gcgtcaccac
    14221 atggattact tggttttcgt aaccttgttt tttataataa gcctttacac gattagccat
    14281 tgtcccattt ggatggtatt gggcaggaat agacgaaacg cctgctggtt tatttactac
    14341 cagaacgtgt tcatcttcat acacgatatc gatcggtgtt tcatccaaaa gtacggtttc
    14401 atgttctcct tcagcaggaa tcacaatggt taccttatca tccttagcta gagaaaacaa
    14461 gacattttct acttgatcat taactttgat ttgaccgcct tgaaatttta ttttcgctaa
    14521 cagacccttt gagatccctt tttcttttaa aaaatatttt acttgctgag aatgatcttt
    14581 ctcataatcc catgtaaact gcatatcaat cctcactaat aaaggcatct ttcacacgat
    14641 gccaaaaatg catatgtcga taagaagcaa aatggatccg ttcttctgct attcgataat
    14701 aaattgagcg gatatttgct tttgcgacat tcaattgatc aaccgttaca agataatctt
    14761 ctgtattttc caattttatt tctagccatt catcatgggc aatcacgatc ggagaaccca
    14821 aagtacgaaa cactcgatta ttcagtgaag caatctctgc caactgaaag gcattgatac
    14881 taggatggat cacagcaccg ccgacacttt tattatatgc tgtcgaacca gtaggcgtag
    14941 aaatcgtcaa tccatctcct ctaaaacttt caaacagctc gtttttgatg tatacatcgg
    15001 ctaccatcgt acgattggcc cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt
    15061 ccggtgtttc atccaaataa ctgatccgaa catccagcaa gggataacta acactttgtt
    15121 cccggttcgt tcgtaagctg tctactagtt cttccagctc gtaatcccgc caatccgtgt
    15181 aaaaccctaa atgacctgta tgcacgccca agaaacgcac atcattcaga caatgactgt
    15241 agtgatggaa cgccgacaat aatgtgccgt ctccgccgac tgagatgacc aattctggct
    15301 catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt
    15361 ctatagtctt tgcttcttta ttatgaacga tcgctacttt catcccctgc ctcctctctt
    15421 tcgctttctt tcttcaaaag cgttcgatag tagacatcat ctgattttcc tcttccatgg
    15481 gagaataacc gctgtgcttc ttggatttct tcacggatac tggacatctc ttcatccaac
    15541 tgataggcag cttctgccgc acgctgaagt cgttcactca tttcttctgg aaaaacgcct
    15601 tgatatttat aattcagcga atgctcaatc gttgcccaga aattcatcgc aagagtccga
    15661 atctggattt ccgccaaaat tttcttctca ccgctgatca gctgtacagg atactcgatc
    15721 accagatgat aagaacggta gccacttgct tttttatttg tgatgtaatc tcgttcttgg
    15781 agaactttca tgtcttttct tttacggatg atctcaacaa cttgatggat atcttctaca
    15841 aactgacaca tgatccttaa cccggcaata tcagacattt cttcttctaa acgtcccatt
    15901 ggaatatgcc gtaattgtga ttttgttaca atactatcga ctggcttgac tctcccagtg
    15961 acaaattcaa tcgggacatg tcgattttgt tcacggaact gtttgcggat cccccgcagt
    16021 ttcactttca attctgatac agtttgttca tatggtgcta aaaatgcttc ccaatctcgt
    16081 tccatagctg atacctctac ttcattaaca ttcattgttt attctaccat atatgcaaag
    16141 ttttttcgta ctcttgattt atgcattgac caaaaaaaga aaattaggca aaatagacaa
    16201 aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat ataaatctat gctgacaaag
    16261 caagagtacg agatattcat cgctcactat caattaacag ataaagattt ccgtgtacag
    16321 actaatattt atttcgatac cgttgatgaa cagctgaaga aacaaaattg tggcctacgg
    16381 atccgtttcg ttggtcatca agctgaatat acattaaaaa cacctgctga aaaagggcgt
    16441 ctcgaaacaa cggatacctt tataacagaa gaagcagaag catttatctt cgaaaaacgt
    16501 ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca
    16561 ttaatccaaa ccggaaagct tacaacaaaa cgagctgaat tccctattga agaaggactt
    16621 ttagctattg atgaaagctg gggagacaat ctacacgatt ttgaactgga actagaggta
    16681 ggggatgcat ctgtgggaaa aattgctttt ataaattttt taaaacgttt ttctttacct
    16741 tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta gaaacctttc
    16801 attttcacaa ggcacttgag ttcatttttt tacttttttc tgttaagtta taggatgtga
    16861 aagaatctag tcactagttg tatagaatct attttggggg agattcatat gatcgaaatc
    16921 tatttatttg ttaatccttt aggggaatac tgtttcgaaa tggaacaaca gttattacaa
    16981 tttatcgaag aagagtacgg gacaccatca aaagaaaaga tgcaatttcg ctttcttcct
    17041 ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa aaggaatttc tcagaacaat
    17101 cttgaggaac gtaatcactt attctcaatg acttattctg cagcattgga ctgcaaagcg
    17161 gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga tcaaattgca agaagcagtc
    17221 ggctgtaatg acataccata ttctcaagaa cttgctgaat ccatatttga agaaatcggc
    17281 ggcgatttga atatgtttaa agaagatcgt cagtctgact tcgttaaaga agtattcttt
    17341 tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac acccttctgc agtcgtttac
    17401 aattatgcct gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac aagtagcctt
    17461 tggagaatca cagagctgtg taaaacaaca gataactcat atcaagtttt taatggtgaa
    17521 catttacata caaatgattc tcagttccat tcaagaacta gccatttaca cttgctttct
    17581 aattaaaaa
//
LOCUS       DACDIP010000051        17562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDIP010000051 DACDIP010000000
VERSION     DACDIP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.051"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(360..680)
                     /locus_tag="IV257_002000"
     CDS             complement(360..680)
                     /locus_tag="IV257_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815102.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1064..2182)
                     /locus_tag="IV257_002001"
     CDS             complement(1064..2182)
                     /locus_tag="IV257_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815103.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2653..3960
                     /locus_tag="IV257_002002"
     CDS             2653..3960
                     /locus_tag="IV257_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8815104.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4079..5278
                     /locus_tag="IV257_002003"
     CDS             4079..5278
                     /locus_tag="IV257_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8815105.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5305..6573)
                     /locus_tag="IV257_002004"
     CDS             complement(5305..6573)
                     /locus_tag="IV257_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8815106.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6736..7383)
                     /locus_tag="IV257_002005"
     CDS             complement(6736..7383)
                     /locus_tag="IV257_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8815107.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(7791..8294)
                     /locus_tag="IV257_002006"
     CDS             complement(7791..8294)
                     /locus_tag="IV257_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8815108.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8417..9181)
                     /locus_tag="IV257_002007"
     CDS             complement(8417..9181)
                     /locus_tag="IV257_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8815109.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9289..9564
                     /locus_tag="IV257_002008"
     CDS             9289..9564
                     /locus_tag="IV257_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8815110.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10002..10952
                     /gene="yidC"
                     /locus_tag="IV257_002009"
     CDS             10002..10952
                     /gene="yidC"
                     /locus_tag="IV257_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8815111.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11131..12105)
                     /gene="lacD"
                     /locus_tag="IV257_002010"
     CDS             complement(11131..12105)
                     /gene="lacD"
                     /locus_tag="IV257_002010"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8815112.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(12102..12560)
                     /locus_tag="IV257_002011"
     CDS             complement(12102..12560)
                     /locus_tag="IV257_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8815113.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(12557..14020)
                     /locus_tag="IV257_002012"
     CDS             complement(12557..14020)
                     /locus_tag="IV257_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8815114.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(14002..14952)
                     /gene="pfkB"
                     /locus_tag="IV257_002013"
     CDS             complement(14002..14952)
                     /gene="pfkB"
                     /locus_tag="IV257_002013"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8815115.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            15095..15843
                     /locus_tag="IV257_002014"
                     /pseudo
     CDS             15095..15843
                     /locus_tag="IV257_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            16109..>17562
                     /locus_tag="IV257_002015"
     CDS             16109..>17562
                     /locus_tag="IV257_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP8815116.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYT"
BASE COUNT         5427 a         3642 c         3046 g         5447 t
ORIGIN      
        1 attaccgaac aaaaactact cctatagtat atcataccgt ttacaaaaaa gctagagttt
       61 ttcgactttt tttattaaaa aaagttttct cagcgattac ataaagtgaa aaaccgaact
      121 ttttttaaaa taaacgacat attgttcagt tggtgttttt ttgctctgat gttcttcttt
      181 aaactaaaaa aattattctt tctgactact tatcggttct gtttcaacgg tcgaggctgg
      241 ggaagattct ttagtaggtc tgatgttctc atggtatttc tgatagccta tgacagttac
      301 cagaagaaca aggcaaacca atacgatcaa cgagattagt ttttcttttt tgctcatatc
      361 tatacctcat aaataacagg atgatgtcca tcatatttct gagtatgagt aaaagcaaaa
      421 gctgcagctg tagaatgtaa gaaatgccag caactttcat aatcacaggt cggacagtac
      481 caaaagtaac caaatcttgg ctgcaaagac cgttcttctg tttcaggaat cgcagaatca
      541 agatctgttg tctgtacata aacatttttg tacagacgtg tgcctgtaat aggagaccgc
      601 tgtgtttctt cagcaaaaac tggctgactt gttaaactta gggatagaaa cagcaaacac
      661 cataacagtc tttttttcat aaagacctcc ttatcgataa gtttttccca attaaaatca
      721 tatctcaaga acgcccgtta ttcacatttt gttcataaat gatgtttttt gtatcataat
      781 cgtcaaaaag aggtcgtgac cacgtcacaa cctctttttt aaacggtgtt ccagaagtag
      841 cttcttcgga aataagtcga aatttcacaa aaatttgaga aataattttt gtcaatctct
      901 tcttatttct tgaagctgac cacttctgtc acaacctctt ccttaaacgg tgttcaaaag
      961 cttgcatctg cggtattagc tcaaaaaacg gaaaaatatg tgaaactatt tttccgtttg
     1021 tttctctaat actcaggtca aaacactgat ttcacaacct cttttaataa cgaagaatcg
     1081 ctgtcagact cttttcatct ggcgcatctt tttgatccaa aataaacact ttcccgccat
     1141 taatgatcac atcatctgct aatgcattca acacttgacg ccgatcatat tctgtttcag
     1201 gatcttcacc gaaaccatca gctaagtttg acgtagcgat aaataaatgt gacacttttc
     1261 cttctttcgc agctggaacg atatcaacta actgatcgat gaatttctta tctaagagtt
     1321 tattgtattc ttttgattct tgagctgcga gttcacttga cagcttcttc gccccttctt
     1381 cgatatctct aaaggatagc tgcgcaggag aagcagaaat cgcaacgtct gcacaataat
     1441 agggattttt agcaattttt ttgaacatcg tctgattttc tggtaatgca aataaataaa
     1501 tcggccattg ttcaggattt tcgaaagaat ccttcaagta cgtatcaaca gcttggtaat
     1561 agttgaccca atcgatctct acttcttcat cttttggatt tacgccatgg aaagttactc
     1621 catctcgggc tccgcctcct ccattagaag agaagttcaa accgccacct gtcagttcag
     1681 atcccaatgc agtaacgaca tcaaccggtg ccccttctgg tagagatact tcttttactt
     1741 gtttgttgct gacctcatac aatttcatgg agtcgcggtt caatcccaat aaataataag
     1801 aatagttgaa ctgcccattt ttgataatag ccagtaaata aggaagatca cttacatagt
     1861 attgatcgtc taccgggata ctcaaacgat ggatataggt ctggtcagca gttataatga
     1921 ccgctacact cgtagttgca ctacgccaaa acgaaggatc tcctaacaaa ttttcaattt
     1981 tttcttggaa cggggaccag tcatgttcgg aatatttttt ctcaaaacgt ttctttgctt
     2041 ctttcgcaaa atttttgaat ttgatctgat ctttttccac atcttgatgg gcgacatgcg
     2101 tattcagcat gatcgtgacg aatggtcctt tagcgccttc tgaagtcaat tgtgtcaata
     2161 attctttatc cttttttgcc ataaacgtac ccctcctaaa aaaatcattc ctttgtttct
     2221 atctaaagtc taccacaaca ctatttttcc atacaataaa acgtatctaa aaaaataaaa
     2281 cggtttctac ggttttaaat ttagctattt ttttaagtta tcttcataat ttcctcaatt
     2341 atttagcccc agtcttcaac tattcttcat gaaatgctga taaactaaga aaaagaataa
     2401 aagaggtgga tcttatgcat ttgattatga ttttcagttt attaagtatt atcagtttat
     2461 ttgctttcag cgcttcccgc ttattagatg cggaaacaca gcctattcct gtaaaagtac
     2521 gtaaaaacga ccgctaaact tccataacgc gcgttcttat cctaaagttt tttcagaaac
     2581 attgaaactc tctcccctct ctgataaaat tttaatttgt agcgaaacaa attaattaca
     2641 aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc tattattggc tagtctcatt
     2701 ctttgccttg cttcttttct accagctgtt gctgtgcaag ctgaagatac gttcaaagta
     2761 aatgcaaaag cagcttttgc tgtagatgcg caaagcggta agattcttta tgatcaagat
     2821 ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca tcggtcttta catcgtcctt
     2881 gatcaagtaa aagaaggaaa actgtcatgg gatgataaag tttcgatttc agattatgca
     2941 gaaaatttga gtatcacccc agatttatcg aatgttcctt tgcataaaga aaacacctac
     3001 actgtcaaag aattattcga ctctgcaatc atccagtcag ccaatgcttc aatggtggcc
     3061 ttggcagaaa aaatctctgg cagcgaagct aaattcactg aacgaatgaa agaacaattg
     3121 aaagattggg gaatcaaaga tgcaacaatc gttaatgcat caggattgaa caactcttat
     3181 cttggcgaaa accgaccaga aggcactgga gaaaacgacg aaaatcaaat gtctgctcaa
     3241 gatgtggcca tcgttgcccg ccatttgatc ttggatttcc cagaaatact ggatgtatca
     3301 agtactacga cccaaatgtt tggcgaaaat acgcaatcac cagtggaaat ggtcaactgg
     3361 aactggatgc tcccaggatt cgttaattac aaggaaggcg tggacggatt aaaaactggt
     3421 acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca ctaaaaacga tcaacggatc
     3481 atcactgtcg tattgaatgc ggacggccat gcggaaaacc cgagtgcccg cttcaatgaa
     3541 acagaccgtt tgatggacta ctgctatgac aattggtcag aaaaagagct aggaaaagct
     3601 aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag acggaaaaga atcaacagtt
     3661 cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa acgatatgga tactggcaaa
     3721 ttaacgatca cgccaacact tgacaagaag caagtcactg acaatgagat ccaagcacct
     3781 atggataaag gtacaaaaat cggtaccgct acgatcacct tggcagacga taaattaggc
     3841 tatttagaag acaacaatgc accttcaaca gagatcatta cggataaaac agtggaaaaa
     3901 gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat ttttcggcaa cttgttttaa
     3961 ttgaacaata agaaaaacat ctttccatcg gaaagatgtt tttttgcttt ttctagttta
     4021 aataacagct tttttcatct attttcgtta tactgtataa gaaaaaggag ttgaatttat
     4081 gaatctaacc agagatctga tctttttagt tctctatctt attggggtat tctctatcgt
     4141 ttattggcga aaacagaaga aggatccttc cttgttcttt tacggcagta cgtggacatt
     4201 tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt tattcgacct tttatttttt
     4261 gattccattg ttgttcttta gtatctttgc tttttcttat ttcacagaaa aacgaaggct
     4321 gctgaatggt cttttattca atgttttcct catcagtttc ggcatctact tgtttgtctt
     4381 gctgtatgaa acgcaaaata ttttccttgg cggactgatc gccttgatca cgattccatt
     4441 gctacttgtc cttttatttg gtatttatgg attgattgtt tttctttttt ggaacggggt
     4501 cacagtttta cgtagagaat cgcattcatt agctaatctt ttaacgttga tactagccat
     4561 ttttcttaca ctgtttcttg tttttgactt tttcttgtta aaatatttgc cgcaatggat
     4621 aaatgctttg tttttctgcg ttcccctgat cttgatctat ctatttatcg tattctacaa
     4681 ctttttgact gtctcattct tgtatcagtt caaccggcct cgatacaatc aagacttcat
     4741 cgttgtgtta ggcgctgggt tgatcaatgg agaaacagtt tctcctcttc tagccaagcg
     4801 aatcaataag gcaattgctt tttatcgggc tcaaagccgt gctacattaa atccgcccat
     4861 cttattgatg tctggcgggc aaggagcaga cgaaaaagta ccagaggcca ttgcgatgaa
     4921 acaatatgcc atggaacaag gaatccctga aagagacatt cttgtcgaaa ccaattcgac
     4981 aaccactttg gagaacatgc tctattcaaa ggaaatcatg gatcagcaaa tgaaagggcc
     5041 ataccgcgcg atctttagtt ccaacaatta ccacattttt agagccggtc tttatgcgtg
     5101 gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca gctttctact acttgcctaa
     5161 tgcgttttta agagaataca tcgccatctt gatgatgaac aaaaaaagac atatgatcat
     5221 ttgcggattg cttttcgcag gctccgcatt cttatcgctg attactttac ttttctaaaa
     5281 aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt tctttcattc tcttaactat
     5341 tggcaaaaag agtatccctt ttttgaccgc tacttgctga gcagaataaa taccgctgtt
     5401 gcctgaacac ataaagctga tcaagcagat cataaagaag tatactgccg cttgtgaacc
     5461 gaacaattcg atccccatca caaagcaggc aatcggtgta tttgtcgcac cagaaaatac
     5521 tccgataaag cccaatcctg caagaaaagg aacagaaaca tggagcaaag gcgctagtgc
     5581 tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact tctccccctt gatatcccgc
     5641 gcctaaagac aaaactgtga agaataactt cccaataaaa tcatagacat gtgcctgtcc
     5701 attaaatgat tcttgaagta acggaagact caatccgaga taacgttgtg tttgcaaaat
     5761 caatgctgct gccacaacta cagctccacc tatcgtattt cgccaaacag gatgaggaat
     5821 ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg ctgaataccc aaccaatcaa
     5881 accaaaagca atcccagcaa caaaaacttt gaagaacagc aacatactcc attcaggaat
     5941 cacacccatt tggtaatgta gatgagaaac accatagctt tctgtcatga aatttgcaaa
     6001 cagtgcagca aagaagctag ggaagatcgc ttctgttcgg actttcccga ttgccaacac
     6061 ttctaatcca aataatgttc cagctaaagg tgtgccaaat acagaactaa aacccgcact
     6121 gattccactg ataatcaaga tttcccgttc tgatttatcc aatcggaaca aacgtgctgt
     6181 attatctgct aacgctcccc ccatttggac tgctgttcct tcacggccaa cagatccccc
     6241 aaataaatgg gtagttatgg ttccaaacag tgtaagtgga ataagtcgca acgggatttt
     6301 ttcttctcca ccatttcctt gatcgatcac taagttgttt ccacgactag aaagaccgcc
     6361 gtgatacgca tagagataag caaaaagcgc accactaact ggtagtagat aaaggagcca
     6421 aggatgtgat agtcggatat ctgtcacaac tgtcaggcta tttaagaaaa aagcagacaa
     6481 gctccccatg aatataccga gaatcgctgt gatcaccagc cacttcagca tgtacactaa
     6541 aatgcgtact ggactcggta attgtttttc cataaaaaat tcctcctcaa taaaaaaacg
     6601 cctactaaag aaaggggctg tccccagact ttagtaggcg tcattagcta tctttcgtta
     6661 gcatttaaag gcgaacacca tcacctattc acttccatta aaagatagca tatttttctg
     6721 taaaaaacaa taaaattatc gttctttctt tacacgttca gcaaattcat agaaaggagc
     6781 caatttcgtt aactgttgat tacattggtc actatcatga caaatataat tccccttttt
     6841 cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga aaaagcgaaa cattcgatgt
     6901 tttctgacag attgcacaga ttcccttatt tatcgttggt gataaagtac cactaatccc
     6961 ttttaatttt tcatcttcgt aatagatcat gaattttttc tgcgtacctg gatcattcca
     7021 gccgatgtac gtatgttctt gcaagtcgat ttcattccaa gctggttgtt tgagtttttt
     7081 cacttttcgg aataatttct cgatttgtgg tttgctaggc tgcataaacg gctgaacgat
     7141 ttgcttcagc tcctccaatg ctcgttctgc ttgcgctttt gtcagtttta cgtctaaaac
     7201 tgtcgttaaa aatgtatcga tttctggcag ttcgtgttct aatatgtctc gaatcttttc
     7261 agttgctagt gcctgaactg tttgtaccgt ttttggatca ttgacagatg agtacgcatt
     7321 taataactgt tctacttctc tttttataaa aaaatattgg tatggtttta ttgtttgttc
     7381 catgatttta aagtcctttc ttctaaccaa agtgcatact ttgtagaaaa aagtgatctt
     7441 ctgcaaagta tgtgttacct gaagatcccg gactctttta aataagatac gttttgcatc
     7501 ttatcatcac cccctttaat gcttattctt ctatgtacat gattcattcg ataaataaaa
     7561 gtaaaccgta tcttgaacag atagtcaact gcaaacagaa aaaaggactg tgacaaaagt
     7621 cgtgtcaaag tcccttgttt cgaatgaatg gtgttcaaaa gcttgcatct gcggtaataa
     7681 gccgaaactt cacaaaaatt tgagaagtaa ttttcgtaaa ttccttctta tttcttgaag
     7741 ctgaccactt ctgtcacaac ctcaatatgg tctattattg gttttacctg ttaaatcgag
     7801 gattctttcc caaagtaacg ttgccatctc ttacgcatac ttgcagacat gggctgagcc
     7861 gcttctttga ctgcgcagta tttgtccacg aatgtcacaa tatatccttc tttatactta
     7921 ggcggtgcga tcgttgcacc ccacatgtgc ttcaaaatga tatctcgctc aagatccgac
     7981 aaatcggtga ttttttctgc attctttaca gcaattcttg gatggatata cgcatgtgtc
     8041 ccctcgtcaa atttcgtcgt acgccaatca tagtagaaca gatcatgcaa aagtccagca
     8101 cgcgctgttg ctttagcatt gccaccccat ttttttgcga gcagataact atagtaagaa
     8161 acactgatgg aatgctccaa acgagtggaa tgaacatgct gtgtgtaatt cgctaatttt
     8221 ttcactgctt ctgtttctaa aagatcttgt acataagaca tatattcttc gtcttcacgc
     8281 catttttctg tcataattat cgatctgcca cctttttgaa tttcattttc ttctgaaatt
     8341 gatctaagta taccaccagt gttcggatta ttctagtcgc tctagataaa atagctaaag
     8401 cttaatagtt ccttaatcat aaagaaaaat gataaagaac gatccctgct gcgactccga
     8461 cattcaatga ttccgcattt cctttcattg ggatatatac gttctgatca gtcaaagaca
     8521 agatttcctg acttactccc tgaccttcgt ttcccatgat tatagcgaaa tcctctgttt
     8581 tttctacttt gtctaatgca tgtgcttctt catttaattc tgtaccgtac accgtaaacc
     8641 ctttgccttt cattacaggg atcacttctg ccaaatcttt ttgtaacaca ggcaaatgat
     8701 aattgcttcc ctgcatgctt cgcagcacct ttgtactata aatatctgcc gttcctttac
     8761 ctaataaaac accagccata ccaaacgcat ctgccgtacg gatcatcgtt cccacatttc
     8821 caggatcctg tacattatct agtaaaagcc agcctcctga aaaatctggc gtatcttcag
     8881 atgttaacgt catcaccgct aagatccctt gcggtgtagg tagatctgac atagcggtca
     8941 tgacctcatc tgaaactaga aaagtagaaa tctctttgtt tacagccagc cagtctcccc
     9001 attctttcat acctctctgt gtcacaaaaa gttcttttat ttctgcatca gacgacgcag
     9061 cttcttcgat cagatggaac ccttccaaaa gatattctgc cttttcttct ctgtatttct
     9121 ttttatgtag cttttttaat tctttgattt ttggattttt tgctgattgg atctctttca
     9181 cattccttca cctcacctga ttatatcatg gaataggtgc ttgagcttgt ttcatcctaa
     9241 aaaaatcggt atcattgatt tataaaacag gaaagaaagg tgacgaacat gcgcaaagtt
     9301 aaaatgaatg tccaaggacg tgtccagggc gtgggatttc gctatatgac caagatggtt
     9361 gctgatcagt taggcgtgac cggaacggta aaaaacgaag acgacggctc agtcacaatc
     9421 gaagcaatcg gaaatgacga catcatccaa aaatttatcg aagaagtcaa gaagtctccg
     9481 agcccaagtg gacgagtgca gtatgtggat gtccaagaag atccgatgat tgaggaacga
     9541 aagaaatttg atgtggtggg ataaggttgt gaaaaggtcg cttagttttg agtattaagg
     9601 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttaat accgagaaac
     9661 tgacttttga acaccgtcga tcaggttgtt tttgaggcgc tttgcctcaa gtattaaaga
     9721 taggtagtaa aaaagctgat ctataacttg caataaattt gcttaaccca tattttccag
     9781 acagaacaaa aaagttttta gttttttata taagaaagaa acacttggaa tcactctttt
     9841 ctttccaagt atttctttct tgattttttg tcttttttca aaatttttcg ctacgaaaaa
     9901 gaaagaatat ttatatgaaa tttctatgaa gttgattgaa ttccatgcgc ttttttagta
     9961 tagttaagtt tgtgtaaaaa ttatagaaga ggaagtattc tatgaataaa tggaaaaact
    10021 ggttattggg atccggactt gtcagtatcc tgctcttttt atctggctgt gtgcggatgg
    10081 attcaaacgg aaaccctgac acttcaggaa tcgtttatcg agtactcgtc catcctatgg
    10141 gccaagcgat tacatatcta gttgagaatt tcaattggtc ttacggctgg gcagttattg
    10201 caatgactgt gatcgttcgt atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa
    10261 caatgatcca atctgaaaaa atgcaagcgt taaaaccaca agttgaagca gcacaacaaa
    10321 agctaaaagc tgcaacaaca cgtgaagaac aaatggcagc acaagcagaa atgcagcaag
    10381 tatatcgcga aaacggattg agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc
    10441 aaatgccgat cttctctgcc ttgtatttta cggctcgcta cacagaaggt atccgtgaat
    10501 catcttttta cggcatcgat ctaggctcac caagtatggt gcttgtggcc attgcagggg
    10561 tcgcttatct gcttcaagga tatatttcaa caatcggtat tcctgaagaa cagaaaaaga
    10621 cgatgcgtac gatgctgatc gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc
    10681 cagccggcgt tacactttac tgggtagtcg gtggtgtgtt cagctgtctg caaacattca
    10741 tcacaaacgt tatgatgaaa ccacgcttga aagcacaagt ggcagaagag ttgaaacaaa
    10801 acccgccaaa acaagtcgtc actccaagaa aagacgtgac agaatcagca aaaccagctg
    10861 acaacaaaca agcaacaaaa caaatcaaac aaccaaaaaa cagtacaaat aacaaaggcc
    10921 gcaacgcagg aaagcagcag aagcggaagt agatagtaaa taagagtgta ataaaaattc
    10981 attaacagtt ttagcaatta aaaatatcga ccaagatttt tgaaaaagaa atctagtcga
    11041 tatttttatt taagtgaatg tataatttga atcatcgatt gatcgctatc taattgcagc
    11101 agcatctttt tatgaagaaa caactttttc ttatctatca acgtagacag gtgttgctgt
    11161 ttcttttaat agtgtactta attccttaat attttgtttt ccttcttcta acatccaact
    11221 actcgctttt tcttcgcctt cggtaacaaa aggtgccact cccttcgccc atgttgctcg
    11281 cccacataag acaccattga atgtcgaacc tgattgtttt gcaaaacgta atgtatcttg
    11341 aaataacgta gcattgactc ccgcacttaa aaaaataaac ggttgttcag tcactttcga
    11401 ttgttcatag aaatacgcgg atgcctctgc ttgactataa caagtctctt gcccatatcc
    11461 ttccacaaat ctcatattca ctggcacttc tactttcaat acatccactt gataacgttc
    11521 ttttgaaaat tctcgcatgg cttcaataac tttatggggt tttacttttg cataggcaag
    11581 agaggacgga tcgccaatag ttgcatcata agaaaggatc tctaaaaaga aaggtatgcc
    11641 ttctgcatcg cattctgaac caatccgttc tataactatt tgtttttgca cattaatctc
    11701 tgctggttca tccacgtcat agtacaataa aaatttacaa gcatccgcac cagcttcttt
    11761 taaccttttc accgaccaaa tattcaataa atcaggtaat cttcccggca ttttcgtatc
    11821 atagccggtt ttttcataag ctagcagcaa gccacattct ttctctctaa gcgctgctgc
    11881 aggcaggccg cattctggat caagtaaaat agctgaggca tagggagtca ggtattgaga
    11941 aaccatctgt ttaaattgga tgatgtcttc tgatgtagct tctttatatt ggttgatcat
    12001 tttcttcaaa gcaccacgct ggtcgattgc taacgcgcta ataattccag cttcatccgt
    12061 caatcgcttc aaacgttcct gcttttctat actcatcttc ttcatagatc ttcacctatt
    12121 tctttagtta atattgtggc tatttctgtt tttgattttg cttttctcaa ctgttctgta
    12181 acctcttttc gcatcaatga tcgtgcaacg agagacaata atttcaaatg agtcgttcct
    12241 gcttctgaat ctggaataaa taacgcaaaa ataaaatcaa ctgactgtct atctaaactg
    12301 ttccattcaa ttcctttgtt cagtgtgacg ataatcatac taggttcgtt gattgtttga
    12361 gacttcgcat gagggatagc aaaacctgac attattcctg tagtaccttc tttttctctt
    12421 tctagaagtt ttcgatatac ttctttttcg tcatgagcga ttcctaaaca gaatgtttgc
    12481 tttgctaaaa acttaaatgc ttcttcttga ctatctaatg gctgttgtaa aaaaatgttc
    12541 tcttcatgaa tgattgtcat gataagcctc ctgctcttcc ttcttcgata actgtacgat
    12601 ttgctgcttt attatccatc ttcttgtctt tatcagttac tcttttcttt tggatcaaac
    12661 ctagtatcac tccaccaacg atcgaaccaa taagaatcgc tgtaacccat tggatttttc
    12721 ccgtcactac aggtaacact aaaaaaccgc catgtggggc tggtacttgg acaccaaaca
    12781 gataggtcaa gacagcagca attgatgagc ctaacatcat cgttggtaat actcggatcg
    12841 ggtctttcgc agcaaacgga atagctccct ccgtaatatg agttgatcct aaaataaaat
    12901 taaccaaacc tgcattacga tcattagtat caaaatattt accaaacaat aaagttgcaa
    12961 aaccagtaat caacggtggt gcaatgcaag ctactgacac gccagccata aaagtcgtgt
    13021 ttccttgtgc caaaagagct gtacctgtta catatgccgc cttattaatc ggtcctccca
    13081 tatcaaacgc acacatgcaa ccaacaatca cgccaagaac aagtggattc gaatgttcaa
    13141 atccagctaa aaagctcatc attccttcat ttatagcctt cattggaccc gccagcaaag
    13201 acatcacgat gccgataaac actacaccaa taactggata taagaagatt gccttcaagc
    13261 catctagttg tttaggtaaa attgtcagta gcttttgcaa aaataagata aaataacctg
    13321 ctaagaaccc agctaaaata cctactaaaa atccagtacc gccactcatg gccacgattc
    13381 caccaacaaa tccaacgact aaccctggac gtttcgcgat cccttctgca atataagctg
    13441 ctaaaactgg aaccatcatc cccattgctg catcgccaac cgtttttaac atcgccgctg
    13501 tagcattgta ttgtgcatgg tctggatcaa aagaatagat tccccaaacg gcaaatgaaa
    13561 tagcaatcaa tactccgcca gctaccacga aaggcaacat atgagagacg ccattcatta
    13621 aatttttata gatgctgcga ccaaaattag caccacttat cgtttctgat tctaaagtat
    13681 caacagcttc attatctgct aagatggtcc cttttcctgc caatgcttcg tcaatcagcc
    13741 gttgtgcatc cttgattcct gtactgacag aaacatcaat gactcgtttt cccgcaaatc
    13801 tttctgaatg gacatctttg tctgcggcaa taatgacagc atctgcctgt ttgatttctt
    13861 cagccgttaa ttcattctcg atcccaattt gtccatgggt ctccactttg atcgtgatgc
    13921 ctttttttct agctgcttgc tctaatgcct cttgcgccat gtaggtgtgt gcaattcctg
    13981 tcggacaacc ggttgctgca attagttgat attttcccat ctttcgaacc tctcttcctt
    14041 gattttgatt tgtttactta attcctccac atccaaaaaa tcagtcagtc ctttacgaaa
    14101 agcagtagag ctgcctgctg ctatgctttt acgtaacgtt tcatcaagcg gtcgtcgatt
    14161 catgtatcct actaaaaaag tgcctaacat ggcatcacct gcacatgctg tattgacaac
    14221 ttttcctttt ggcgagtttc ctcgaatcac cctgtctttc gtaaaaagaa tcgctccttt
    14281 ctcaccaagg gataacaaaa cattttctgc acctgactct accagtcgtt gtccataaac
    14341 aaagtaatct tcatctgtaa ccatttttcg tccgaaccaa gaagaaagtt cttcctcatt
    14401 cggtttcagc aaaaatggtc gatagggcaa acaatccatg acctcttgat cactgctatc
    14461 aataatcaaa tgaatctcct tttcctgaca aatccgactg atttccacta aaaccttcgg
    14521 agacattcct cttggtaaac tcccagaaac acaaagataa tcctctgctt gtaatgctct
    14581 tatttgattt aaaaaattat ggacagctgc ctgcggtatt tcaggtcctt gattcactaa
    14641 tttgtattct tctctcgttt gattgacttg tgtaaataca ttgatacgcg tcataccagg
    14701 cacttcaata aactctgttt ggatttgttt cttctgtaaa tagtcttcta tatattttcc
    14761 tgtgaaacca gcagaaaaac ctaatgccgt acttgggaaa tctagcatct ttaatactag
    14821 tgacacattt actccttttc cgtttgcctg aatgtcatcc tcctttgtac ggtttaccat
    14881 aaaaggatgc atctcctctg tttcaataaa tagatcaatt gccaaattca tcgtgcatgt
    14941 gtagatagac ataaccaatg acctccttga atctattatg aaatacgcga aagagaaaat
    15001 gttttcttaa tcgttccaac acaaagaaaa cctttacatt tataaacaca tcgtgctatt
    15061 ttttaactaa cttttagtga aagaaggaaa gaatatggat cacaaattaa gtgaaacaga
    15121 acagtacctc tggaacttta tcgaacacca catcctagag atccctaact actccatcgt
    15181 gaaactaagt gaacaagcaa atgtctcaac agccaccatt gtgcggacaa tgaagaaaaa
    15241 aggctatgaa ggatttactt cgtttaagca ccatctaaaa gaaggagaaa acaagaacat
    15301 caatttttct ttttagataa agtagacaaa ggtatccgtc gagccattct aaaaaatgaa
    15361 caagaagtgg tacgtacgat caatatgttg gaaatcggga acattgaaga tgcaatacaa
    15421 agaatcaaat ttactgaccg ggtttttgtc tttgcccgag gattttcaga aatgattggt
    15481 caagagatgc tcgtaaaact tcaattaaca ggaaaaaatt gtcaaatgca tactgatcca
    15541 gagattatca aaagcatcag tcggaaacta actaagaaag acagcgttat ttttgtttct
    15601 ttgaacggcg agacaaaaga actcgtcacc gccgcaaaga actgctatga tgcagagatt
    15661 ggttcgattc tagttacagc caatcattcc tctacactca aagattattg cgagataaat
    15721 ctcgtaggtt tcaaatcaga aggttcttat ttccctgatt atgaagtacg ttcacgcttg
    15781 cctctccaga tcatcgcgag aatcctgctt gacgcctatg tattgcgtat gagggagaat
    15841 tgagaagcag tttattatgg ttggttacag tttaaaacat cacatttttt attctttata
    15901 ttgtatggac tcacgtaaac tttttatgta taaagttatt cacaatctat aaagttattt
    15961 taagtaacaa aaaataattg aaaatactta aaatcaaaaa atatcttttt aatcaataaa
    16021 aaaattgaca gttttattaa taataagcta ttctgttata gaagaaaaaa ttgtttgtta
    16081 ttattagcaa caaaaaaagg agtgcatcat gaaaaagtta tctcaaaaac gtttagctca
    16141 aatcattatt gattctagaa aatctctgaa aatgacacaa caacaacttg cagtagctac
    16201 tggtatcaac cgtgccttaa ttagtcgagt agaatctctc gattttatcc cgtcaataca
    16261 acaattagaa tcattagcag aagttctaaa ttttgaacca acagatctat ttataaatga
    16321 atcaaataaa tcaaacagaa caaaacatca gtcgaagaat attgctgtag ccggtactgg
    16381 ttacgtaggt ctttccattg ctactcttct tgcccagcac aatcaggtaa cagcagtaga
    16441 cattcttcct gaaaaagtaa aacaaattaa caataagaaa tctccaatac aagacgatta
    16501 cattgaaaaa tatctagctg aaaaaaatct taatttaatt gcaacgactg atggtgcagc
    16561 cgcctataga gaagccgatt ttgttgtcat tgctgctcca acaaattatg atagtaaaca
    16621 aaattatttt gatacttctg ctgtagagtc agtaatcaaa ttggtgttag aagtcaatcc
    16681 aaaggcaata atggtcatca agtcgacggt acccgtaggc tataccgaaa gtattcgtaa
    16741 aaaatacaat actaaaaata ttctattcag cccagaattt ctcagagaat ctaaggcatt
    16801 atatgacaat ctatatcctt cgcgaattat tgtcggcacg gaccaaactg acaaaacttt
    16861 acgagaagcc tctcacactt ttgcagattt actccaagaa ggagcaatca aaaaagatat
    16921 cgaaactctt ttcatgggat tcacagaagc tgaagcagtt aaattattcg ctaatactta
    16981 ccttgcactt cgagtatctt acttcaatga gctggatact tatgcagaaa tgaaaggact
    17041 aaatacacaa gaaatcattc acggggtttg cttagatcca agaattggca cacattataa
    17101 taatccatct tttggatatg gaggctattg tttaccgaaa gatacaaaac aacttctcac
    17161 taactataac gatgttcctc agaatatgat aagtgcaatt gtcgatagta atcgtacaag
    17221 aaaagacttt atagcagatc gagtgttaga acttgctggc acgtatgagg ctaacagttc
    17281 gtggaatgca gaaaaagaaa aagaggtagt cataggtgta taccgtctta caatgaagag
    17341 taattcagac aacttccgtc agtcatctat tcaaggagtt atgaagcgaa ttaaagcaaa
    17401 gggggcaact attgtaattt atgaaccaac acttgaaaat aacactacat tttttggttc
    17461 aaaagtaata aataatttag atgattttaa aaatatcagt cacagtatta ttgctaatcg
    17521 ttacgatagt aatcttgatg atgtaaaaga aaaagtatat ac
//
LOCUS       DACDIP010000052        17008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDIP010000052 DACDIP010000000
VERSION     DACDIP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.052"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..127)
                     /locus_tag="IV257_002016"
     CDS             complement(<1..127)
                     /locus_tag="IV257_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP8815117.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="IV257_002017"
     CDS             complement(231..542)
                     /locus_tag="IV257_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8815118.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="IV257_002018"
     CDS             complement(544..741)
                     /locus_tag="IV257_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8815119.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="IV257_002019"
     CDS             complement(1135..2862)
                     /locus_tag="IV257_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8815120.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="IV257_002020"
     CDS             complement(2855..4048)
                     /locus_tag="IV257_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8815121.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="IV257_002021"
     CDS             complement(4235..4879)
                     /locus_tag="IV257_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8815122.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="IV257_002022"
     CDS             complement(4973..5107)
                     /locus_tag="IV257_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8815123.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IV257_002023"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IV257_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8815124.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="IV257_002024"
     CDS             complement(7238..7711)
                     /locus_tag="IV257_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8815125.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="IV257_002025"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="IV257_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8815126.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="IV257_002026"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="IV257_002026"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8815127.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="IV257_002027"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="IV257_002027"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8815128.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="IV257_002028"
     CDS             complement(11758..12897)
                     /locus_tag="IV257_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8815129.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="IV257_002029"
     CDS             complement(13206..13919)
                     /locus_tag="IV257_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8815130.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IV257_002030"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IV257_002030"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8815131.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="IV257_002031"
     CDS             15108..16640
                     /locus_tag="IV257_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8815132.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17008)
                     /locus_tag="IV257_002032"
     CDS             complement(16831..>17008)
                     /locus_tag="IV257_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8815133.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3495 c         2910 g         5324 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaataca
//
LOCUS       DACDIP010000053        16909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDIP010000053 DACDIP010000000
VERSION     DACDIP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.053"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(282..896)
                     /locus_tag="IV257_002033"
     CDS             complement(282..896)
                     /locus_tag="IV257_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8815134.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1502..1879)
                     /locus_tag="IV257_002034"
     CDS             complement(1502..1879)
                     /locus_tag="IV257_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815135.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2179..3060)
                     /gene="galU"
                     /locus_tag="IV257_002035"
     CDS             complement(2179..3060)
                     /gene="galU"
                     /locus_tag="IV257_002035"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8815136.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3103..4122)
                     /locus_tag="IV257_002036"
     CDS             complement(3103..4122)
                     /locus_tag="IV257_002036"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8815137.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4755..5759)
                     /locus_tag="IV257_002037"
     CDS             complement(4755..5759)
                     /locus_tag="IV257_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8815138.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5772..6821)
                     /locus_tag="IV257_002038"
     CDS             complement(5772..6821)
                     /locus_tag="IV257_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8815139.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6838..7659)
                     /locus_tag="IV257_002039"
     CDS             complement(6838..7659)
                     /locus_tag="IV257_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8815140.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7646..8425)
                     /locus_tag="IV257_002040"
     CDS             complement(7646..8425)
                     /locus_tag="IV257_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8815141.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8442..8912)
                     /locus_tag="IV257_002041"
     CDS             complement(8442..8912)
                     /locus_tag="IV257_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8815142.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8924..9343)
                     /locus_tag="IV257_002042"
     CDS             complement(8924..9343)
                     /locus_tag="IV257_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8815143.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9424..12192)
                     /locus_tag="IV257_002043"
     CDS             complement(9424..12192)
                     /locus_tag="IV257_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8815144.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            12392..12616
                     /locus_tag="IV257_002044"
     CDS             12392..12616
                     /locus_tag="IV257_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815145.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            13395..15110
                     /locus_tag="IV257_002045"
     CDS             13395..15110
                     /locus_tag="IV257_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815146.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            15113..16876
                     /locus_tag="IV257_002046"
     CDS             15113..16876
                     /locus_tag="IV257_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815147.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4993 a         3479 c         2875 g         5562 t
ORIGIN      
        1 agaatccata tggattcttt cttgtctctt tttccactac tttttatttt tctgacacta
       61 catattctta taactcattt ctcataactg tgaaacaact tataaaaata acaaacttta
      121 aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa taactaaaac aaaaaaagta
      181 gtcgttgtta atcctcatta ataatcgttt tctttttttc aaacgctcca tttttatgtg
      241 gaacattatt tttttaagga actttgtcaa catgtggata actattttcc acttgtgtcc
      301 tggactagct gttttgctgt aaaatacaaa acttgctgca gcaaaaagat cgttgcaaga
      361 gaggctgttc cgcagatcgc tggatctaga tttggcaaag tcaagactgc atcatgtaaa
      421 ttggcgtggt tgtccaaaac caaatcagca aattcaaata atcgcaaacc actgctatgt
      481 tgcgatttga tacttcgtga caaatcaaat ccagtaacaa tgattaatgg atgaccattt
      541 tctttgatcc actgagctag ttcgatgatt gccgggtttc ttccttcatt cgaaagaaga
      601 agaaaaatgt cttctgattt gacagaaagc tgatcaataa ttgcctgtcc tgcaccttct
      661 agcgtttcat agacacctcc ggctggattt tcaatcaata tacttggtac aagatccggg
      721 gtttgatccc aaaattcaaa tgctacacca cttagtgtgc ggctagcata tatctgcaat
      781 ctaccacctt gattaatttg tttcgcgatc caagtgccag cttgaagcat cagcggctct
      841 tctatcctta atttctcttc caattggtga taatagtcaa aataacataa actcatttat
      901 ctctcccgcc tagctatact tgacatgaat agaaacagct gcctaatcta aggaagtaca
      961 taaaaaaaca gtagttagac agtaacacat gtggtctttg ctgtttcaaa cacaaaaaag
     1021 gcattgaacg aatacgccca ataccttgac atagcaaata aaacaacgtg cagaccatgg
     1081 aaaatatcta cataggggta gaattttcac cctagattag gtacaataaa ttaggtcgat
     1141 gcactgcatt gactatggca aaatgatcgg tcagggtgta tgtgtgtgac tggtcattcc
     1201 ctgattctta gcgccaacta agggtcaggg agcctttttt tattcagctg tttctatctg
     1261 aatttattgt aagtaatctc ttatccaagt gcaagcaaga aaatacaaaa aaacattggt
     1321 atgatttata tatatataaa taacataaaa aaaggaatgg cttatcccga tacaacaagc
     1381 attcctttaa aaaagattct tttatataag tgaaaaaagc ttttttccac caattaaata
     1441 aatctctcat ctcaatagtt tatccattat tttatctttt tttgcgtata cctactaaat
     1501 attaccgtct tcttctcgtt cttctacttt gttcgagaga atctgtacta gaaaaagtag
     1561 ttgtatgtct accttttctg cctatccgag actttctttt catatatggg atataaatga
     1621 ttagatacag tacagcaaaa aataaagcaa atacgcaagc ttcccaacta cctaacgcca
     1681 attcaaccat caaggtagca accccaacgg atgctgcata ccagataaaa atcagaaacg
     1741 tttctttttt tctgaccatg cgaaacatga taaatagact aataagcaat agtggcaaca
     1801 caaatatcca attgcttcct tccatcagcc acttgagtat atcaagcagc gactgtgcaa
     1861 cagcgactaa ttttcccata gtgaacacac accttttttt ataattaaaa aattcgcctt
     1921 tttattctac tatgaatctt ctggcttggc tttcatttct cttatgtaat cattttcttt
     1981 tatacaactt aaaaaaaaat taaaccatta gcataatatt ttctgaatct attcacgaaa
     2041 gtctttttct atctttcgtt ttccacgact caactgatct agagtcaaaa aactaaaacg
     2101 acagttgcgc aaatgacgca caagctgccg ctttaataac aaatagcctt tcattctcag
     2161 caggccactt atttccagtt aactttctgt tcttagcttt ttactcaact ggataatata
     2221 tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca tattcaatat ttgtcttcat
     2281 atatcctaac ttatcgccta cgtcgtatct ttttccttta aactctctcg catagaccgc
     2341 ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt atctcgtttc ctgctcctgg
     2401 ttcttgagtt tctaatatct caaatatttt aggagtcagt agataacgtc cgataatggc
     2461 taaattactt ggtgcttttc ccatttcagg tttttctaca aaccctttta ctctgcaacg
     2521 acccttttcg ctatctgttt taggatcaat aattccgtat ttagatgttt cattttctgg
     2581 tacacgcatg acggcaacag cggaagcttg tgtttcttca taatccatca tcaattgttg
     2641 agtcaatggg atatcatctt tcatcaaatc atcccccagc atgacgacaa acggttcatt
     2701 tcctataaat gcttttgctt gaagcacggc atcccccagt cctcttggat gagattgacg
     2761 gatgaagtga agattgactt gagtcgtttc ttcaactaaa gcgagtaatt ctgttttttc
     2821 cttttcccgc aaattgtttt ccaattcgat atttgcatca aagtgatctt caatgggtct
     2881 tttttctttt cccgttacga tcaagatgtc ttcgattccc gaatccaatg cttcttccac
     2941 aataaactgg atcgttggtt tatccacgat tggaagcatt tcttttgcca tggcttttgt
     3001 tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg actgcttttt tgaccttcac
     3061 ttgctgttcc tcctaaacga cgattaacta ttgatcatct atttaacgaa gagaaaattc
     3121 attctccatt tttccgtcac gtaacatgat ttcttttgct gcttttttga tatcttctcc
     3181 attatacaaa acattataga ttgtttgagt aattggcatc tcaacaccta gctgttgagc
     3241 tagctctact gctgcttttg tcgttgccac accctcaacg accattccca tgttttctag
     3301 aacttcttcc agcttatgtc cctgtcctaa caagttaccg gcacgccaat tgcgagaatg
     3361 aacacttgta caagtaacga tcaagtcccc cacaccactc aaaccgataa atgtcagagg
     3421 attggcaccc atcgcgacac caagacggct gatttctgcc aatcctcgag tcatgattgc
     3481 tgcttttgca tcatctccga atcctaatcc atggatagcg ccggcaccaa ttgcaatgat
     3541 atttttcagt gctgcaccag tttctacgcc aatcacatca ttatttgtat aaatcctgaa
     3601 ataatcattc atgaacagtt tttgtacata ggctgctgct ttttgatcaa tacttgcagc
     3661 tgtaattgtt gtgatatcat gtactgccac ttcctcagca tggctaggac cagataatac
     3721 aacaattgcc tgtctttttt cttctggaat ttcttcagcc aaaacttctg agattcgttt
     3781 atgcgtatct tgttctagtc ctttacttgc atgtataatg attggcttag tattcatttt
     3841 accaataagt tcctgtgcga ctgaacgaat cgcttttgtt ggaacaacaa ataaaatagc
     3901 atctgcgtta tccactgctt ctgccaattc tttatatccg ataatcgatt ctgggatcgt
     3961 caaatcaggt aaaaaatgct gatttgtatg atacttattg atttcatcaa tctgttcagg
     4021 tacatttccc caaatacgga catcatgccc gttttctgcc aatgtttgtg ctaaagcagt
     4081 gccccaagat ccaggaccaa gaactgcgat tttttgtttc atggatattc ctccaaataa
     4141 ataattgata atttccatta tagtatacat ttcttaaagt tgggactaat ttccattgtt
     4201 tttaagtgtt tgtttacttc ttttatcgtt tttacttgaa ataatcaatg atatgttaca
     4261 atagtctaca ttttattttt acactcgtta tgctttgctt gttcctaaga ggaagcagtt
     4321 ttgacaatgt tactaccagc attactatta ggaggttagg aaacttctcc tagtgagtat
     4381 tgatttaaaa tacataagag acagattgaa catttctaaa tctgtcaggt cctgcaacgg
     4441 acagattgtc tctgttgacg gttgacatag gaacttttgt tttagtcaac attctggata
     4501 caaaacgcta tgtaaactgg cgcaactcgt tgtacttgga cttttactac ggatccatgc
     4561 aacaactaaa tagtactatt gcataacaaa aaatggttgg cacccacatg tcccattaaa
     4621 cagattataa gttgaactgt agataatgca aaaaatgtgt tttataacgt aattgatgcc
     4681 aatattttaa aaaaataaag ggagcagtta tcgctgctct ctttattttt tatattcttt
     4741 ttatccagtc tatcctaata atcaaactga cgatagtagc ggcgtatctc taatggatgg
     4801 cagttcaact gctccacatg agcatcgatg cgttgtgtca ctacatgcat caataagaaa
     4861 gacaaaacac gtccgcgata tttttcactg ataccttcta ctggatagtc ttttgtgtct
     4921 atcaatgtat aattgctgca aatttttggc aaaagtttag ccacacgttc agataacact
     4981 ctctgttcat cttcgcctaa aaataatgtg actggggttg tttcttctac gatctctaac
     5041 gtcccgtgaa gaaactcggc agcatggata gatttcgatg gaagccagct ttgttcttcc
     5101 cagtaacaca tggcatatga ataaacagct ccccattgat tccctgctcc aataaaataa
     5161 tgcatactat cacgtcgatg tttttctgca aatgataagc caaatgcatc tgcttttttc
     5221 tctgcttcca ctaaccctgt tggtaaataa cgttcgagct gttcatagta ttctgaataa
     5281 gcttcgaatt ctccattcaa atacatcaat cgatctgctg ccataaaaaa tttgatttgt
     5341 tctgttccag gagctggata agtgaccaca aaatcgcata gctgagacaa cttactgttc
     5401 gctttatcga taaatccaat cagtgtcgct cctactttct tgatttgttc tactgctttc
     5461 actacttctt gagttgtacc ggtaacagag gacaaaataa cgatagaatc ttttgttaat
     5521 cttttgtttc cagtagtgta atattctgca gcatgctgta caaagacagg caagttgctt
     5581 ttcccattca tataagtgac agcttgcatc gaggaagcat acgttccacc gattcctaga
     5641 taatagacag cgtcaaattt ttccgcataa agtttatcga tgatttcttc tacttgcgga
     5701 cgtaaagcta gtgcgccttt tattgctttg atttgttctt cttcattgaa tttcagcatg
     5761 atttccacct cttataattt acttttcaag actgtatcaa agtcttcaaa aatctttttg
     5821 ttcaagtcaa tacctttagc tactgcggtt ttataagcga atagttgaaa cggaacgaca
     5881 agcgctaata cagacagata ttcatcacat tctattgcta atccaagtgt tttctcgttt
     5941 cttgcttttt ttgtggtaat ggtgagagtc tctccaacgt accgactcat atatctttgc
     6001 aacttttgcg aacgttcctt atttacactt tccccttcaa taaagaatag caaatgcgaa
     6061 gcatctgcct ccaggtatgg gccgtgcata taagcttcta actcaaatcc ttgtgacgga
     6121 acgcggattg tttcagtcaa tttcgtctct gcttctttag ctgttcccca gtttggacca
     6181 tatccaatgg tgacgaaacg ctgtgctaaa cgaaataaac tttgatatcc atcaaaaaat
     6241 tcttccgttt tttgaataat cgctggtaaa tggttgatta ttttgcgcaa ctgttgcatg
     6301 tagtcttgtt ctattttttt gctgatttta tttttactta ttccaattcc tatccctaag
     6361 agtagtaact gtaaaacagt cgcagaatag ccttttgtaa cgaagcctac tgattctacc
     6421 ccggcaccta aatcaataat ttgtgcagct tctaaagcaa gtggactttg cacgtcattt
     6481 gtgatagcaa caacaggtaa cccttgtttt tgtatcattt ttactgcttc aacagtagaa
     6541 gcacttttcc cgctttgtga cacagcaatc acagtatcga ttgaaggaga aagttttcca
     6601 taattgaaaa aataaaaagg ttcttgaata tcgacagtaa gatcaccata agattccaat
     6661 gctggcttcg ctgctaaaca agcgttataa gatgaacccg ttgctaagat cagtaaattc
     6721 actgtttttt tcatatgttc tcttgtcgaa aaatcattct ttttcaagat cgtacttagt
     6781 acttcctttt cttcatagat ataatcttgc atcgtaaaca ttttctctgc tcctttttta
     6841 aaaaatccct atccacgaac caaaaattcc aaagaaagct agtccgatca agatcgttaa
     6901 tggtttcact tctttcttca atagataaaa cactatgcca aatgctccaa gacataagat
     6961 atttggaacg atatcatcaa aaatactttg aacagtcaca gcattttctc ctgaaccgat
     7021 ttttaaagga atattgatgg tgatcatcgt ggcagtcatt gctccaacga ccatcagtcc
     7081 aataatagac gcgcctaatg tcaaactttc catcataccg tttgcctgga tctttttcaa
     7141 aaaacccgtt cctaagcgat atccccaacc tgttgctaga tagcgaaaca gaatgtgcgg
     7201 aatattgaaa atcaaaatga acaagatagg gcctaaaata tttccttgca tggctaaaga
     7261 ggtacccaca cctgtggcaa tcaatcgcaa agttccccaa aagaaagaat ctcctaaccc
     7321 tgcaagaggt cccatcaaag atgttttgat actatcgatc gcagtcacat caaattcagg
     7381 atcatcctta ttttcttcct ccattgcagc cgtgattcct aaaatcagtg taacgatatg
     7441 tggggtcgtg ttaaaaaact ctagatgccg tttcaaagct gctgccattt cttctttcga
     7501 ctgatataac tttttcaaaa tcggaatcat tgcataaaca taagccatat tcatctgacg
     7561 ttcataattc caagaaaact ccatttgaaa agaacgccaa aatacgtgat ttagctcttt
     7621 tttcgttatt ttgctgtttt tgatctcaga attcttcatc ttcttctacc tcccctgcag
     7681 ttgtttgaag tggtagttga cggacattca taatattgac taccacaact gctaaaatcg
     7741 caccaaaaat agctactccg gtcacaggaa tctctaagta agaagctata gcaaaaccta
     7801 agaaaaaata tggtgcaact tgtttgttga tcagcaaacg agcaagcatc gcaaaaccaa
     7861 gagctggaat caaccctgta gctactgaca accccttttg aacgaagtca ggaatcatat
     7921 ttaatagatt tccgatcgta ttgcttccca ccatataaga aaccattacc acaacaccaa
     7981 gcatcaagga aagaccgata ccggcaagca agtgcatatg ctcgactcct ttataatttc
     8041 cttcttctgc ataattgtct gctttatggt tcataattgg aatcaaaagc cctaagtaaa
     8101 tatttttcaa tatcaatgtc aacgtagcaa tcggcaaacc tagtaaaagc gcggtttctg
     8161 taccggctcc agctgtaata gcaaaagccg tccctaaaat cccaccggtc acaacatcag
     8221 gtggaatcga agcacctact gagaacgatc cgataaatgc taactctaaa gttgcgccca
     8281 taatgatccc agtttttaca tcacctaaca cgattcctgt aaacagcccc gtcacaatcg
     8341 gacgagaaag caacgatgtc cctagtgcat attctgattg tgcgacaaat gcaacaattc
     8401 ctaataagac tgcttgtaca atcattgagg ttcttccttt cttacaaaac ttctcttaca
     8461 tccacttttt tatctgctgg tacttgacgg atttctactt ctatttgttt gtcaattaat
     8521 cgtttcaagc gttcctcctc ttctggtaaa atatttaccg cttttccgat gctgcgagta
     8581 ccaggttttg ccttgatccc tcctaagttg atggttttga tttctggata cgcttctgcc
     8641 agcttatatg catcctcgat cgattccact acgatgaata acttatattt atctgtcacc
     8701 ccgcttttca acgcggcaat cgagtcatca attgatttga tcactagttt gactccttgt
     8761 ggcttggcta atttgatcgt tgtctttcgt atctcattcg taggtacatc atcattagca
     8821 atcaaaatac agtctgcgcc tattgtttgt gtccatgaaa aagctacttg tccgtgtaat
     8881 aaccgatggt cgactcttgt caatacaatc atttttcttc ctcctaaaat tcgtcttctt
     8941 ctattttctg attgagccgc tcgttacaaa actggatcat tttttttgaa tcagttagca
     9001 cctgctggat caaaacagtt gtctctactt cagcgtccaa ccgagatact agctcaatca
     9061 atagtggcaa attcaatccg gcaacgagat agacatgatc ccgattgata tattgtaaga
     9121 attcgttgtt gacacttccg ccaaaaagat ccgtcagaac aatgatttct ttatctttgt
     9181 acctcataaa aagctgcctt actgtttccg ctaaatcaaa attctcatca gtatagcaat
     9241 cgaatacagt aatagaatga tttttcccat agatcagctc taatgaatct aatatccctg
     9301 aagcaaattt tccatgggaa gctaagataa tttctctatc cactttttca tctcctcttt
     9361 tcacttacac atactaaaaa agcaagttgc atgccaaaat tagtggcaac aacttgctta
     9421 tgttcaaaat tcttcttcta tcgagagaaa ctctgtatct tcactcaaaa tatcatagat
     9481 ataggcaatc tcagaattgg ggatcatgac actataatgc tcttcaatga cactaaacgc
     9541 agatttaata gcagataacc cttctttttg acactgcttc catttctcta atccttgata
     9601 ggtttcgatt ggcatttgtc ggatcaatcg ttcaatgaga cagctcacat ggacatacaa
     9661 cgctaatttt ttgggattgc tgatgcttcg attcaactgc acctctaatt ctctcataaa
     9721 atgatcgatt tctcccatca ccttctctgt atctagaata gtcacggaat ccagtacttt
     9781 ttccaatgaa aaattacgaa tgatgcgctg attgaatatt tccttctctg aagcattcat
     9841 agtagtgtcc aaccattcag ctagtacaga catcttttcc cccgagatga ggtcttctaa
     9901 tgaaagatag tcgatattgg tgatatcagg atctgtggtg ccaatcattc ctacgacttc
     9961 gtatatggaa aataatgggt cactgttttt cttatctttt aattgctgat attcatatgg
    10021 gatgattttc aactgacaag ccgtaggtaa gcttttttct agcagttcac ttaagtggac
    10081 cgccgtaccg attccagtga agcaagtagt aatgagtgct ttgggtttgt tttcctctgg
    10141 atagagcagt cgccattctg gctggatctg tcgaacagtc tcttctgcta tctcttctaa
    10201 tgatacattt ttttgcaggc attcccctac tgtgattgct aaaggtgtcg tcacattatt
    10261 caaaatcaaa agcggtacgg ataactgttt tttaaaaaac tgatggattt ctttcagaga
    10321 tcccatgtct actaatatga tcaatccatt tgaaacatcg ctgttttcgc aataatcaag
    10381 gatttctgaa gcaatctgct gtggtgtgac atctagtggc atatcaaatg attcaaatag
    10441 atcctcttgt aataaacgat ttactacatt tgccatactg ctagcagttg cataaccgtg
    10501 agcagcaatc atcgcttttg gcagtcgttt ttcttttgac catttcgctt tttttagata
    10561 gatcgtcagt aaaatccgat ccatacttgt gtcttcaata tctaatttga tcttacttaa
    10621 ttccagaatt cgttggacat agtgataact agatggataa gactgagaaa cttgttgttc
    10681 taattctttg attagttgga taatatcatg ttcttccggt aaccatttga tagtgcttcg
    10741 ctgatagaga tagtaactca aggcatagac actgtttcca tcgaatttga tttgataggc
    10801 agtttccatt tgtcgaaagg tctctcggat agattgtgtc caataaacca gcagctcgtg
    10861 tttttcttgt ctactggtct caaacagcaa aaaatcaaat aattgaatca cttcttgctt
    10921 taattttcct tcgcaagaat atagcagaca gttatttttc ttgaaatcaa ttaggatttg
    10981 ctcaaaactt ttaacgattc gctgctgttc gggctgttgc ttctccatta attgctctaa
    11041 ggtctggtct tgcgtaatat agattccttg ctgtgctgta tttttagtaa taggctgcgc
    11101 aagtagttct ttttgcaaat gatggatcgt gacatggatt tcaggctgtt cacgctgttc
    11161 agcatatgct tttgctgctg aaactttgac gatatttttc agctctccta tgtttccgga
    11221 taatttctga cttgttaaaa gtgacaaagc tggtccactg actgacaaag ggctccccat
    11281 ttttctttgt tcttcgagaa aaaaagagta aatcagttct agccgttcat tacgactacg
    11341 ctgattaagt gcaggtagtt cgatacggat aggtattctt cgaatgaaag ttgttaagaa
    11401 atgactagta atctcttcag tcgtagcaaa aaagagacgt gttttgatcc ggcgtgacaa
    11461 attaggttct cccatacgat aaatcacacc ttgatcaaga taggtgaaca gtttttcctg
    11521 accttctggt gttaagcgat gtacctcgtc taaaaacaag atcccaccat ctgctgcttc
    11581 aaaagcccct tttttatttt cttctgcacc agtgtacgct cccttaacat gtccaaacaa
    11641 attgctagtc aaaagttcag gattatctgc atattgtgca caattaaaag tgatgaatgg
    11701 tgcagaatct tctagcagat catgaagcag acaatactga tagaccaaat taactaaata
    11761 acttttacct gtgccacttt cgcctgtaat caaaagtggt aatccgccat ctggatagta
    11821 aagagctgtt ttgatttgtt cgatactttc tttcaaactg gcgtcgtatc cgatcataaa
    11881 agagaataag tcttttggtt gcgcgaaaaa aggctgttcc tctttgagat catcgataga
    11941 aaaataaaaa ttcgtgcgta acttaaaaaa ttgtttttca aaagcttctt tgtggaaata
    12001 aatcactggt cgagaattga ttttgactag tagttgctca tcatttaact ggttcaaata
    12061 atgactgact gtgtttcttt ttaccccaaa atgatcagct aacctttgtg ctgtaaagac
    12121 ctcgttcata tgttctaaat cgaaaaaggc tgtctggttc tctaagtaac ataaaatttc
    12181 cttcttcaac aacttattca cccctcctcc atcatagaaa acggattcat tttgtcaata
    12241 aaatctttaa taaagaattg aaaaatatat cttattatag acagcagcct acttatccct
    12301 tataattttt attctaaaaa acgtacagtt ccttccagtt tttatactgg aatttcaaca
    12361 tatgaaagaa taaggaggct atgactattc aatgaaatcg gataaatata aaaacaggaa
    12421 acatacttcc tggaaaactt ttcgagaaat catcggatct tatctcgctg tagttgtatt
    12481 tttgcctttg ctttatatgg ctatgacaga acaaactcgt tttattggcg tagatacgca
    12541 atatatagcc gaaacaggat ttttattcat ctttatcttg atttttgcgt tctataaatc
    12601 cttccataca aaataagatt cacagatttc aactttcctg tctgttttta gctattacaa
    12661 atccttatat gtggtataac aacaatttat tggcataatg aggaaaaacg agttatgatg
    12721 ctttcagaga gtaaagaggt actgttgtca ccatcctttt tattgattac gtacaccact
    12781 cttagttttt tcccattgat tacgaaaaca aggtagaaac attgatgttc ctaccttgtt
    12841 ttcttttttt attttcagat caaaatctca agtagctttt gacttgatgt cgggaggttg
    12901 tcttcgttcc atcaaacctg atagaatctg cgccaaacca tagttttctc cactattatt
    12961 tattcctcta agatagtacc cacaataaac gaataaatat ttttctcatt ttcatactcc
    13021 ttctttgaaa atcaattaga tttctctcaa cccacttttt tacaaaaatt acgtagcaaa
    13081 aaagattgca agaacaaatt aaaaaagaag taaatagagt ttcatctata aacatttact
    13141 tgatatattt tttatgcaga attcatttat agaaaaatta agtaaaatag acgttcctat
    13201 gaatgaagag actggcatgt tttctcattt tctttttttt aaaaaaaata gtctattcaa
    13261 tttctaaagc ttcttcataa taaaactttc atttatgaaa aggcttgttc tcttttcatt
    13321 ctaaatttcc taagctacaa tgtggtagta cttgtttttt caattgtgga caaataaata
    13381 ataggaggaa tcatatgatt cagttattac gttatgcaaa agactatcgt aagcaaatca
    13441 ttttgggccc gttcttcaaa tttcttgaag ctgtcttcga acttgtcctg cctttaatga
    13501 tggcttcctt gatcgataat ggactaaaaa tgaatgaccg aggaaaaatc attgaaatgg
    13561 gcttatggat ggtcgctatg tctgtgattg gtctgatttg tgctatcatt tgccaatact
    13621 atgcttcagt agcttcacaa ggatttggta cagagttacg taatcagcta atcaaaaaaa
    13681 tcaatacttt ctctcacaac gagttgaatc attttggaac agataccttg atcacccgaa
    13741 tgacaaacga catcaatcag cttcagctag ctttagcaat ggtcattcgt ttattgatca
    13801 gagcaccgtt tttaagtatc gggtcagtgg tcatggcttt tgtgatcgac tgggaagtag
    13861 gtctgttttt cttagcatta ttgcctattt tctcaatcat tttatttttt atcatcaaaa
    13921 aaaccgttcc tctctaccaa aaagtgcaag aaaaactaga tcagctcaat gaacacgtta
    13981 gtcagaatct tagcggtgtt cgtgtcatcc gcgcatttgc gcagacaaaa aaagaaacag
    14041 agaagttcga taaaagcaca aatgatctag cgaacaatta tttacgtgtt tccaatatct
    14101 ctgccctatt atcacctgct acaaccttga tcatgaatgt cgggatcatc tgtttgctga
    14161 cagttggcgg gatcaaagta aatattggta gcttgcaaca aggacaagtt cttgctttga
    14221 tcaattatat gaatcaaatg cttttggcac tgatcgttgt ttcaaacctc gtcgtgatct
    14281 tcacacgtgc cgaagcttca ggaaatcggg tgaaagaggt gctagacaca gaaaattcga
    14341 ttcttgaagc tgaggtcagt actactcccg atttttcttc tgaagcaatt atcgcttttg
    14401 accatgttga cttccgttat actcctgatt ctggtctttc tctccaaaat attacatttc
    14461 agctgaagcg aaatacagtc cttggaatca ttggtccaac cggaagcggg aaaacgactt
    14521 tgacacaatt gattccgcgt ttctatgatg tcagttccgg tacagttata atggacggaa
    14581 tggatgttcg ttcttggcag ctggatcatc tccgtcagca aattgctctt gttccccaaa
    14641 cttctgtact atttaccggg actattcgtg aaaaccttca atggggtaag aaagacgcca
    14701 cagaaaaaga atgttgggaa gctctgaaaa tcgctcaggc agaagaattt gtccgtcagc
    14761 ttccaaatgg tttagataca aaagtcatgg aaaatgggaa aaatttctct ggcggacaaa
    14821 aacagcgact gacgattgca agagctttga ttgccaagcc agcattactg attttagatg
    14881 actctttaag tgcgttggat tatcaaacag acttggattt gcgtaaggcg ctacagactt
    14941 atcttggcag tacagtgatt attatctccc aacgtgtccg ctctattcaa gaagcacatc
    15001 atatcttagt gatggatcaa ggtaagattg ctgctcaggg tacccatgaa gaattacttg
    15061 ctcaatcagc agaatatcag gaaatcgtcg catcacagga ggaagaatag ttatgaaagt
    15121 aaaaaaatta tccttgcaga cgctccgccg attcattcct taccttactg cctatgcaaa
    15181 agacatttgg ctagctgtga tactaggaat catcaatggg attgcaacag ttgtgatgac
    15241 gttacaaatt ggtcaaagta tcgatcaaat gattggagaa ggacaggtcc gcttcaatcg
    15301 actgttccat ttacttattc tattcggtgg tatcgtttta atcaacgtag tcagtcaatg
    15361 gctcatccaa gtgttaagca atcggttagc ttacttatcc gttgcaaaat taagaaaaga
    15421 tgcatttgct catctgaatc gattgccact tcgttattat gatcaaaact cccacggaaa
    15481 tatcgtcagt cgatttacaa atgatatgga caatatttcc attgcaattt ctgcggcctt
    15541 caatcaatta ttttctggag tggcaatcat cattctttcg tttattttta tgatgcgtct
    15601 aagtccactt ctgacattgg tcgttctttg ttctacacca attattttcc ttgtgagttg
    15661 gatcgttgct cgtgcttcac aaacaaattt cgatagacag caaaggatta ttggggatat
    15721 ctcgggcttt gtgaccgaac gaatcggtaa ccagaaaatc gtgaaagctt ttcagcaaga
    15781 agaagctagt caacagcaat ttgaactgct aaatgctgat ctatatgaaa aaggacaacg
    15841 ggcacaattt tcttcttcct tgacaaaccc gtcttctcgt tttgtagatc accttgctta
    15901 tttatcgatt ggtttagttg gcggcctatt ggcattgaag agtggttcgt caatcactgt
    15961 tggtgtgatt tccagcttta cgatctactc aagtcagttt tctaaaccat ttatcgaact
    16021 ttctggaatc acgacacaac tgcaaaccgc tttttctggt ctagaacgag catttgattt
    16081 gatcgatcag ccagaagaaa agcctgattt gccagatgcc tatgccttgg aacccaaaaa
    16141 tatcaaagga aaagtctctt ttgatcatgt ttctttttct tatgacccca agcagcgttt
    16201 gatcgaagat ttcaatttta ctgcttatcc aggtcaaacc attgctatag taggaaaaac
    16261 aggggccgga aaatccacac ttgttaattt gctgatgcgt ttttatgaca caacaaaagg
    16321 aacaattaca gtcgatgacc atgacatccg ctctatccaa cgtgatacat tacgtaaaag
    16381 ttttggaatg gtgttacaag atacttggct atttgacagt acattgcgag aaaatcttcg
    16441 ttttgggaat ccggaagcaa gtgacgcaga aatcaatgat gccctaaaag cctcttatat
    16501 gtatgaattt gtagaacgtt tgccggaaaa attagatact aagattggcg gtcaagggtt
    16561 aaagatatca gatggacaac gccaactgtt gacgattgct cgaacgatga tcagtaatcc
    16621 ccctatgttg atcttagatg aagctaccag ctcagtcgat acattgacag agaaaaaaat
    16681 ccaacaagcc ttcttagcaa tgatggaagg aaaaaccagt tttgtgattg cccatcgcct
    16741 gtccacaatc aaaaatgcgg accagatatt agtgatggat cagggagcta ttgtcgaaat
    16801 gggcacccat gatgagctgc ttcaaaaaga tggttattac cgacggctct atcaagcaca
    16861 attcacagca caataaaaac aagcagtctt ctgggatggg aagactgct
//
LOCUS       DACDIP010000054        16885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDIP010000054 DACDIP010000000
VERSION     DACDIP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.054"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(258..1763)
                     /locus_tag="IV257_002047"
     CDS             complement(258..1763)
                     /locus_tag="IV257_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8815148.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1760..2446)
                     /locus_tag="IV257_002048"
     CDS             complement(1760..2446)
                     /locus_tag="IV257_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8815149.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IV257_002049"
     CDS             complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IV257_002049"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8815150.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IV257_002050"
     CDS             complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IV257_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8815151.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4467..5036)
                     /locus_tag="IV257_002051"
     CDS             complement(4467..5036)
                     /locus_tag="IV257_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8815152.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5222..6124
                     /locus_tag="IV257_002052"
     CDS             5222..6124
                     /locus_tag="IV257_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8815153.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IV257_002053"
     CDS             complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IV257_002053"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8815154.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IV257_002054"
     CDS             complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IV257_002054"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8815155.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IV257_002055"
     CDS             complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IV257_002055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8815156.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12692..12877
                     /locus_tag="IV257_002056"
     CDS             12692..12877
                     /locus_tag="IV257_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8815157.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12939..14135)
                     /locus_tag="IV257_002057"
     CDS             complement(12939..14135)
                     /locus_tag="IV257_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8815158.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14305..15741)
                     /locus_tag="IV257_002058"
     CDS             complement(14305..15741)
                     /locus_tag="IV257_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8815159.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16034..16843
                     /locus_tag="IV257_002059"
     CDS             16034..16843
                     /locus_tag="IV257_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8815160.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4954 a         3539 c         2950 g         5442 t
ORIGIN      
        1 aaataataat atagcaatct ctttataaaa ttgcaataga aaattcccta tttttattta
       61 ttatttttaa acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact
      121 tacatttaat taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat
      181 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
      241 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      301 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      361 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      421 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      481 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      541 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      601 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      661 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      721 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      781 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      841 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      901 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      961 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
     1021 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
     1081 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
     1141 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1201 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1261 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1321 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1381 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1441 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1501 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1561 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1621 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1681 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1741 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1801 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1861 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1921 ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1981 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     2041 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     2101 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     2161 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2221 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2281 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2341 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2401 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2461 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2521 tatagtcatt ttattatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2581 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2641 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2701 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2761 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2821 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2881 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2941 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     3001 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     3061 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     3121 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3181 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3241 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3301 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3361 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3421 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3481 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3541 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3601 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3661 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3721 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3781 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3841 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3901 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3961 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     4021 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     4081 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     4141 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4201 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4261 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4321 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4381 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4441 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4501 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4561 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4621 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4681 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4741 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4801 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4861 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4921 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4981 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     5041 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     5101 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     5161 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5221 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5281 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5341 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5401 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5461 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5521 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5581 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5641 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5701 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5761 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5821 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5881 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5941 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     6001 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     6061 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     6121 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6181 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6241 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6301 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6361 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6421 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6481 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6541 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6601 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6661 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6721 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6781 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6841 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6901 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6961 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     7021 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     7081 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     7141 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7201 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7261 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7321 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7381 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7441 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7501 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7561 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7621 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7681 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7741 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7801 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7861 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7921 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7981 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     8041 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     8101 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     8161 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8221 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8281 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8341 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8401 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8461 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8521 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8581 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8641 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8701 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8761 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8821 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8881 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8941 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     9001 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     9061 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     9121 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9181 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9241 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9301 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9361 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9421 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9481 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9541 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9601 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9661 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9721 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9781 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9841 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9901 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9961 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
    10021 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
    10081 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
    10141 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10201 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10261 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10321 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10381 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10441 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10501 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10561 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10621 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10681 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10741 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10801 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10861 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10921 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10981 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    11041 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    11101 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    11161 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11221 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11281 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11341 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11401 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11461 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11521 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11581 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11641 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11701 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11761 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11821 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11881 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11941 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    12001 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    12061 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    12121 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12181 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12241 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12301 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12361 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12421 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12481 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12541 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12601 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12661 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12721 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12781 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12841 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12901 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12961 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    13021 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    13081 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    13141 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13201 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13261 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13321 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13381 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13441 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaagag accgatcaaa
    13501 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13561 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13621 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13681 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13741 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13801 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13861 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13921 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13981 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    14041 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    14101 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    14161 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14221 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14281 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14341 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14401 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14461 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14521 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14581 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14641 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14701 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14761 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14821 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14881 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14941 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    15001 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    15061 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    15121 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15181 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15241 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15301 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15361 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15421 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15481 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15541 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15601 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15661 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15721 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15781 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15841 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15901 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15961 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    16021 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    16081 tcaagattcg atgattacat ctaaaacaaa agagactcta aaaaaagcaa tcaatgccgg
    16141 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16201 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16261 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16321 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16381 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16441 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16501 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16561 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16621 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16681 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16741 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16801 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16861 caaaaaagag gttgtgacaa ttttt
//
LOCUS       DACDIP010000055        16615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDIP010000055 DACDIP010000000
VERSION     DACDIP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.055"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="IV257_002060"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="IV257_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8815161.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
     gene            complement(680..1666)
                     /locus_tag="IV257_002061"
     CDS             complement(680..1666)
                     /locus_tag="IV257_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8815162.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1689..2255)
                     /locus_tag="IV257_002062"
     CDS             complement(1689..2255)
                     /locus_tag="IV257_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8815163.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2269..2685)
                     /locus_tag="IV257_002063"
     CDS             complement(2269..2685)
                     /locus_tag="IV257_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8815164.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3142..5076)
                     /locus_tag="IV257_002064"
     CDS             complement(3142..5076)
                     /locus_tag="IV257_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8815165.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5378..6313
                     /locus_tag="IV257_002065"
     CDS             5378..6313
                     /locus_tag="IV257_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8815166.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6403..7506
                     /locus_tag="IV257_002066"
     CDS             6403..7506
                     /locus_tag="IV257_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8815167.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7519..8472
                     /gene="menA"
                     /locus_tag="IV257_002067"
     CDS             7519..8472
                     /gene="menA"
                     /locus_tag="IV257_002067"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8815168.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IV257_002068"
     CDS             complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IV257_002068"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8815169.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10887..12128)
                     /locus_tag="IV257_002069"
     CDS             complement(10887..12128)
                     /locus_tag="IV257_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8815170.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(12146..13069)
                     /locus_tag="IV257_002070"
     CDS             complement(12146..13069)
                     /locus_tag="IV257_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8815171.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13290..15782)
                     /locus_tag="IV257_002071"
     CDS             complement(13290..15782)
                     /locus_tag="IV257_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8815172.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15789..16256)
                     /locus_tag="IV257_002072"
     CDS             complement(15789..16256)
                     /locus_tag="IV257_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8815173.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16392..16465)
                     /locus_tag="IV257_002073"
     tRNA            complement(16392..16465)
                     /locus_tag="IV257_002073"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16429..16431),aa:Arg,seq:acg)
     gene            complement(16523..16596)
                     /locus_tag="IV257_002074"
     tRNA            complement(16523..16596)
                     /locus_tag="IV257_002074"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16560..16562),aa:Pro,seq:tgg)
BASE COUNT         4907 a         3477 c         2956 g         5275 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
      601 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      661 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      721 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      781 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      841 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      901 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      961 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
     1021 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
     1081 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
     1141 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
     1201 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
     1261 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
     1321 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
     1381 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
     1441 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
     1501 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg attgcttgtt
     1561 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1621 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1681 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1741 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1801 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1861 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1921 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1981 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     2041 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtctcg ttttttcatg
     2101 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     2161 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     2221 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     2281 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     2341 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     2401 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     2461 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     2521 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2581 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2641 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2701 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2761 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2821 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2881 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2941 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     3001 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     3061 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     3121 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     3181 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     3241 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     3301 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     3361 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     3421 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     3481 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3541 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3601 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3661 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3721 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3781 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3841 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3901 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3961 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     4021 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     4081 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     4141 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     4201 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     4261 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     4321 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     4381 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     4441 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     4501 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4561 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4621 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4681 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4741 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4801 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4861 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4921 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4981 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     5041 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     5101 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     5161 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     5221 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     5281 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     5341 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     5401 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     5461 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     5521 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5581 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5641 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5701 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5761 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5821 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5881 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5941 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     6001 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     6061 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     6121 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     6181 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     6241 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     6301 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     6361 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     6421 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     6481 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6541 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6601 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6661 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6721 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6781 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6841 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6901 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6961 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     7021 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     7081 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     7141 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     7201 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     7261 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     7321 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     7381 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     7441 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     7501 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7561 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7621 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7681 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7741 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7801 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7861 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7921 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7981 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     8041 ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta
     8101 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     8161 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     8221 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     8281 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     8341 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     8401 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     8461 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     8521 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8581 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8641 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8701 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8881 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8941 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     9001 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     9061 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     9121 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     9181 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     9241 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     9301 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     9361 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     9421 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     9481 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9541 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9601 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9661 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9721 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9781 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9841 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9901 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9961 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
    10021 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
    10081 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
    10141 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacaaa
    10201 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
    10261 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
    10321 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
    10381 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
    10441 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
    10501 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10561 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10621 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10681 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10741 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10801 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10861 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10921 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10981 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    11041 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    11101 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    11161 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    11221 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    11281 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    11341 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    11401 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    11461 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    11521 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11581 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11641 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11701 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11761 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11821 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11881 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11941 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    12001 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    12061 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    12121 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    12181 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    12241 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    12301 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    12361 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    12421 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    12481 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12541 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12601 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12661 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12721 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12781 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12841 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12901 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12961 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
    13021 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    13081 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    13141 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    13201 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    13261 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    13321 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    13381 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    13441 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    13501 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13561 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13621 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13681 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13741 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13801 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13861 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13921 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13981 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    14041 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    14101 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    14161 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    14221 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    14281 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    14341 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    14401 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    14461 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    14521 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14581 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14641 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14701 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14761 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14821 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14881 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14941 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    15001 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    15061 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    15121 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    15181 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    15241 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    15301 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    15361 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    15421 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    15481 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15541 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15601 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15661 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15721 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15781 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15841 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15901 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15961 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    16021 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    16081 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    16141 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    16201 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    16261 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    16321 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    16381 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    16441 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    16501 acaaaaagca agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc
    16561 caaaccaagt gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DACDIP010000056        16088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDIP010000056 DACDIP010000000
VERSION     DACDIP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.056"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(268..609)
                     /locus_tag="IV257_002075"
     CDS             complement(268..609)
                     /locus_tag="IV257_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8815174.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(677..2848)
                     /locus_tag="IV257_002076"
     CDS             complement(677..2848)
                     /locus_tag="IV257_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8815175.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            3059..3916
                     /locus_tag="IV257_002077"
     CDS             3059..3916
                     /locus_tag="IV257_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8815176.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(3987..4844)
                     /locus_tag="IV257_002078"
     CDS             complement(3987..4844)
                     /locus_tag="IV257_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8815177.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(4829..7531)
                     /locus_tag="IV257_002079"
     CDS             complement(4829..7531)
                     /locus_tag="IV257_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8815178.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(7547..8833)
                     /locus_tag="IV257_002080"
     CDS             complement(7547..8833)
                     /locus_tag="IV257_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8815179.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVE"
     gene            8983..10029
                     /locus_tag="IV257_002081"
     CDS             8983..10029
                     /locus_tag="IV257_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8815180.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            10031..12184
                     /locus_tag="IV257_002082"
     CDS             10031..12184
                     /locus_tag="IV257_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8815181.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(12657..14108)
                     /locus_tag="IV257_002083"
     CDS             complement(12657..14108)
                     /locus_tag="IV257_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8815182.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(14105..15829)
                     /locus_tag="IV257_002084"
     CDS             complement(14105..15829)
                     /locus_tag="IV257_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8815183.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(15822..>16088)
                     /locus_tag="IV257_002085"
     CDS             complement(15822..>16088)
                     /locus_tag="IV257_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8815184.1"
                     /translation="FATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
BASE COUNT         4434 a         3338 c         2873 g         5443 t
ORIGIN      
        1 aggagctatg acaagacttt tgtcgtagct cctatttctg aataaacggt gttctcctgc
       61 caactcctcg gtcacaagtc acaatagtca tcaaacatga gaagctgttt tttgaaattg
      121 ttccttgtaa ctcagagtta aacggtaggc tcacgacctc ttggtaaatg gtgttcaaga
      181 gacagcctat cggcaataag aagcaatttt gtcatcgctc aaggccgaac gtctctttca
      241 caccctctgt tgtttcataa aatagtttta ttcgctgtaa aggtcgcgaa ctggtgtcat
      301 gatgatacgg ttcaagtcat tgatgattgt actgaagcct tgctcttttt gcatcaagtc
      361 attgattact tcttctgatt ggattttcac agccatttct tgtgctttca ttgcatcttc
      421 gtcgctgaac tcttgtccac tcatttgttt ttcttgtaat tcgatttgca tcgcttggaa
      481 gtctttgaac agtttgtgtg cttcttcgtt tgcttgtact ttctcgtacg cttctttcaa
      541 cgctttgaat tctggtaatt cacggatctc acgttcgatt tgattcgcgc tatcgtagat
      601 gtttgacatg ttatgccctc ttttctttta gtagttactc tctattgtaa catggggtta
      661 ttcgttttca aaggggctac tctccttgat atttccgcca gaggtcttta gccgcttcac
      721 caattccctg aacgccttct ttcaccatat cgccgaagtt tttcagccct tcttttgctt
      781 gttcaccata ttgattcaag ttatcttgcc aatttccaga ctctccatta gaatttcctg
      841 tgtttccaac ttgatctgca gctaccactt gtcctcctgt ggcataggca tctgctacac
      901 taaatcctgt ttttgaagta tacggaagga tgccttctgc cgctgatttg aagactttcc
      961 cgacaacatt tccacttgtc ccttccaaat agtgcagctt gctggattct tcgtaaccca
     1021 tccatgtcga aatcactacg tctggcgtgt aaccgatcat ccattggtca tttgcttttg
     1081 tggcatcgaa attagtttct gtggttcctg ttttccctgc tatcgtataa cctgcaggat
     1141 tcgcagcagc cgctgttcca tttgaaaatg taccaagaag catgctggtc atttcatcgg
     1201 ctgtttcctt tgatatgacc tgttttggtt ttttgtttgt gttatcgacg atcaccgctc
     1261 cagtcgaatc aacgattttc gtgataagat gcggcgtata catctgacca tcattggcaa
     1321 atgcactgta agcacccgcc atgatcaatg gagatactcc tttttccagt ccaccaaggg
     1381 ctaagccata atacttatca gattttgcta aaggcaggcc gaattcttct gctttatcat
     1441 atcctttttg taaaccgatc tcatgcaaaa gccatacagt aggcaaattc aaactttgtg
     1501 caacagcttg atacatcggt acttctcctt ggtatgtccc gtcaaaatta tgcgcatcgt
     1561 aataactttg cggttcgtct ttcaagatac tggaaggctt gtatccagct tccagtgctg
     1621 gtgtatatac gcttaatggt ttgatcgttg atcctggtga acgacgcatc tgtgtagcaa
     1681 agctgaatcc tctaaagaca tgatctcctc ttcgtcctac tagtgcttgt acaccacctg
     1741 tattcggatc taaagctact gaagcagatt ggaccatggc accatctgaa gcatttgctg
     1801 ggaataatgc actgttttga taagtagctt ctaattgctt ttgataatcc tgatctaaag
     1861 ctgtatagat tttatagcct ttattcatga catcttcttc atcaagacca tactcgttaa
     1921 ctgcttcatc gatcactgca tcaaaataat aaggataacg ataaccagaa tctgaattat
     1981 cgtatgtgtc attaagatac gcgctgatat ctgtctggct ttctgcttgt tcttgttctt
     2041 ttgtgatttt tccgttttct tccattacac ttagtactgt gtttttccga tcattcgcat
     2101 tttttgggtg atcgatcgga ttataaatac caggaccttt cagcattcct gccaaagtag
     2161 cagcctcaga cagcgtaaca ttcgttgcat ctactccaaa atactttctg gaagcgtctt
     2221 ggacacccca taccccgttg ccaaaatagg cattgttaag atacatggtc aagatttctt
     2281 ctttgctgta cttcttttcg atctcgattg ccaaaaataa ttccttcgct tttcgatcaa
     2341 acgtttggtc taatgtcaga tacgcatttt tggcaagctg ctgagtgatc gtacttcctc
     2401 cgccccctga tgttccgcca gacaagacca ttcggactgc tgcacgggca atccctttga
     2461 tatcaaaccc gtgatgctcg taaaaattcc tatcttctgt cgagattact gcatcttgga
     2521 tatagggaga aatcgcgttt aattctacat atgttccttt ttgggcgtac agtgtgccag
     2581 cttcttcgtt tttcttatca taaatgactg tcgattggct taatcctgat tttaacgttt
     2641 cgacatttgc ttgttttgct aaaataaaga ggtagatact tgtcactaag acacagatca
     2701 aaccaatcag caaaatcaat ttgttcacat gatatttttt ccagatgcgc tttcgccatc
     2761 gatggaattg tccgagatat ggtctgatcc aacgccaaaa actgatcaaa gcagctttta
     2821 tcttttctaa aatgctttga aaatccattt gcttcttctc ctgtctattt gattcaatcg
     2881 tcttatttta ccatagtgaa agagtgtttt cttcctgctt aagttgtagt tcaatatgtt
     2941 tttttacgtt agtgcaagta gagtaacata agaatgaaca gaaaaatgta agttttttct
     3001 taaatatgtt ttaaaaatgg tacaattagt ctgcgattgc actgaaagga gtacacttgt
     3061 gaaaatcacg atcactttac ctatgaacca tcctgaaaca acaattcgcg acttgcttga
     3121 aaaggaatgg ttagttccta gaaaggttcg acactttttg cgtatacgta aaaacgtttg
     3181 gatcaatcaa caacctgctt tgttccatca tgaagtaaag ggcaatgacc agatcacatt
     3241 gttattcgaa gaaacagatt ataattatca agaagttcaa ttaggagatg cgaagcacat
     3301 ccgtgtttta tatgaagatg agcatttgat tatagtggat aagcctgcag gaatgaagac
     3361 tcatccaaat gaaccgacag aaaacagtac cttactcaat catttagctg cttatctcga
     3421 cactaaacag caacgacctt acgttgtcca ccgcttggac aaagaaacaa gcggtgccgt
     3481 tttatttgca aaaaatccat ttgtcttgcc gatacttgga agaatgttgg aacaaaaaat
     3541 gatctatcgg agatatcaag ccatcgtatg gggagaaatt gccaaagata tgaccatcac
     3601 caataaaatt ggacgagatc gccatgatcg gagaaaaagg gtcgtagatg ataaaaatgg
     3661 tcagagcgcc ttgacacatg tcactgtcaa caaagtactt tctgaacaaa cacaagtcta
     3721 ttgtgttttg gaaaccggac gtacacacca gatcagagtt catttagctc ataagggaca
     3781 tccgatcgtt ggagaccctc tctaccaaaa tagaacagct gaacgactga tgctccatgc
     3841 attagaactg cacatgatcc atccatttac tcaagaaaaa atagtcggta aagctttacc
     3901 tggattatgg gaatagaaat aaaaaagaac agagtcttat cagcagataa tgataagact
     3961 ctgttctttt tgtgtaattt gtctgcttac ttttgatgtt cgtgttctgc taacagcaaa
     4021 ctaccgataa tcccactttc attcggcagt ccccaacgaa cgatatattc acttacttga
     4081 ggtgtttcca tataaccacc catcaattca acaaattgct gacggatttt ttgaaggaga
     4141 tgatcctgat tcattacccc accgcctaaa ataatctttt caggtgctaa cgtcaacgta
     4201 tagttgatca gtgcttgagc aatataataa gcctgaatag cccaaacagg atgatcttgc
     4261 ggcagatctt gccctttgat ccctgtgcgt gcctcaatag acggtcctgc tgctaatcct
     4321 tctaaacaat ctttatgata aggacaagta ccttcataat ggtcatctgg atggcgtttc
     4381 acccaaatat gtcccatttc aggatgtgcg atccctgaaa aaatttcttc attgatcacg
     4441 acacctgcac cgattcctgt tcccacggta aggtaaatac aactattttt tccttgtgct
     4501 gctccttgct tcaattcccc atatgcagca gcattgacat ccgttgtcca agcagcagga
     4561 atattgtagc gttccttgat tgtgccaaga aaatcgaatt gtgcccaccc ttttttggga
     4621 gttgccaaaa cgtatccgta attatctttt gttttgtcga taccaatcgg gccgaatgag
     4681 ccaatcccga ttcccttcaa ttcataacga tcaaaaaatt caaatacctg atccaatgtt
     4741 tcttctggtg ttgtggtagg gatacttaat ttttctaaaa tttctccacc atctgaaacc
     4801 gcacacacaa attttgttcc gcctgcttct attccgccat acattcgttt tcctcctcga
     4861 atccgattgt ccgaatctca taaggttgga ttattgggac aaccgcttgc ttcgttggtt
     4921 cttcaagcag attcaatatg acagtttttc cattgtcttt agttatttcc aatttttcta
     4981 aatgactaga catattgaaa cctcttacta ctactttatc actaaacttg ctgcgtttca
     5041 aagctgtaat tgcaaacgtt tcacttttta tcgttaaata ttgttgtttt gaaatcaatg
     5101 ttccttcatg atgtttgatt tgttgagtgg aaaaaggaat ctgtgctgcg tatgcatgca
     5161 aataagtaga gaatttttct tctggtccat gtagttcaat gctgtaattg aaccgatgct
     5221 cacctagaca ctgtgcttct ggtgttggaa aatatcccca atcgcctagt tctcccacac
     5281 tgcgaagtag cgtcactgcg atttgtccgt cttctgttac ttcatattca ttcaaaccaa
     5341 agtttccgac tgttacaccg tactcttcat catgaatatt gacaaatgct tgctgatgtt
     5401 gcggattcgt ggggttttcc caacttttag acacatggtt tgggcgcttc accacttcaa
     5461 aaatactatc tgcttcgtga tgttccacat gtaactttgt aggaaacaag acacgtaagc
     5521 gatgatcttt catctgattg tcgagagtcg tttcaaagtc gattttttta ctatcttttc
     5581 tgattgtcat gattgttttt atttgaagga ttcgtttttc tttggatcgt tctgcttttc
     5641 gataacgaaa ttccacaacc atctgctgtt ctttttctaa acgctcgtct gcagaaacag
     5701 ggatttccat ttcctgtaga atacttattt ttgctatttc cggtgtatct actaatactt
     5761 ttacttcaga gttttctttg ttactggata ggattggctg atcacagaaa ggttgtttaa
     5821 agatatactc atttcctata tcgccgatat cttcaaaaat atgcagatta ttcaactttc
     5881 gtttgttttt gcgatcttcc attgtaatgg taccattttt ttcaatcgtc aaatgtagag
     5941 ggccgttttc gatgattcgt ccactctgat gaatcattgt ttccttttct tctgctttcg
     6001 cttccccttc tacaagcgcg aatgtttccc aagaaaatgc tggcatgttt tctaatggta
     6061 aagtcacttt gatcattctt gccatatatg gcacacggaa acggtctttc ggcaaatcat
     6121 aaccaaattg gacagtttct tcagataaaa tagctggtat agtggcccca ctttttgttt
     6181 ctacatgata cttacgttta ggcaaatttt ctaactcttg atatagttgt tcaggtattc
     6241 cttcagcgaa tctttttctt tctaattcga tggttatttc tgcttcacca gttttatcca
     6301 ttcctgcagt atttacaatg acaaatggaa agctgtcttt tgggaatccg gtcgtatcga
     6361 ttgctgcggt cagctgttct aaggaatctt gtgcaagata ctttccaact tcatctgctt
     6421 tttcaaaacg aggtatcatt tcccgatgga cagaatcgac tgaacaaccg caaatactgt
     6481 cgtgaggatg attttgcatt aatgtcttcc aagcataatc taattgatca tggggatagt
     6541 ttccactaac ttcataagcc atcgtcgcaa gtggttctgt aatattttct agctgtcgct
     6601 ggaccttggt attccattgt tttaagtaaa cccgagcaga tgctgtattg gctaaagtat
     6661 accagccatc cgtttcttga cttgtcaatt ctccttctac tgagcctaag tcttctggca
     6721 catctttttg aactgcttcc agatagtcag taaaattaga atgaataaac tcgtaatctg
     6781 gaaacagttc atttgcgaga tggattgctt ttgatatatc tttttgtacc ggttgatgat
     6841 caacaccatt catcatcaat aagtggttcg tagaggcata ttgttctgca tccgctaatt
     6901 tttgcttcca aaaggcaaga gctgcttctt tttctactgg aatctcattc ccgttgctat
     6961 accaattggc aaatagcaga ccaaagattg ctgtttgatc aggtcctttc caccacatct
     7021 ctgagtattg ggaagaatag ttttctgctt ccaaaacttg attatcaaag ccaattggtt
     7081 ttacaccacg gccaaacgct gctgctgaaa taccagcttg tttcattaat tgaggcgtct
     7141 gtcccatatt tccaaatgta tcgggaaaat agcctaacat gacaggggtt ccccatttac
     7201 ggctttcttc catgccaatc agcatatttc tcacgttcga ttccgagctg ataagaaaat
     7261 catcttgcaa gatataaaat ggtccaattc tcaattttcc ttcattgatt gcttgttgga
     7321 ttgcctgtct tttttctggc cttacttgaa gatagtcatc taaaataatc gtttgcccat
     7381 ccaaatgaaa actgttgaag gacggatcaa cttcaaatag ctctagtaga tcgtctatca
     7441 attcaatcag tcgcatgtga tgctgttcgt aagccatgta ccattcacga tcccagtggc
     7501 tatgagaaat gatatatact ttctttttca tttacgatac ctcctactat tctactctaa
     7561 tatcaaagta atccatgact aattcgcaaa acatcatatt tgcccaagaa aaccattctc
     7621 tcgtataatt ttcaggattg ttcacatcga atccttcgtg cataaggtgt gttcctgcgt
     7681 cagttgctac aagtaaattc aatatccgct ctttttcaga tttgtctgac gttgtcattc
     7741 cttccatagc taatgcaatc ggccaaacgt atttttctgg cgtatgggag gatccaattc
     7801 cttttgcgta ctttccttca tagaaatatg gattttcttt actcaacaat gttcttcttg
     7861 tttgcagata atcttcctct tgttcagaac aatatcccaa gtaaggtgcg gcgatcagat
     7921 tgggcacatt actgtcatcc atgactgtgg cgtttcccat tccatctact tcatatgcat
     7981 aaatcgtttg ccctttttga ttaaccgttc gtccgtgatt ctgaatcccc tcttggatct
     8041 ctttcttcaa tttctgtgtt tgctgtacaa tttctttatc atcgagtatc gtttgaaaaa
     8101 tttcttccac ataacctaac acgaccaccg caaacatatt ggatggtacc agatatccat
     8161 aacggcaagt atcatcgcta ggacggaaac cagaccatgt cattcctgta aaagctgttt
     8221 ttgcacctcg cccgtcattg atcagcgtat cttcttttct tgtcgtgtct cgtacaaatg
     8281 tataaggaga gtgttcatga tcttgctctg ttttgaacac gtccaagatt tttttgatac
     8341 cttcaataaa agattgattg aattgatcag tttttcctgt atttttgtaa agaagatagg
     8401 ccaactgtat tgggtagcat aaagaatcaa tttcatattt tctttcccag atccaatcgt
     8461 tcatttttgt atgatctgtt tggtggccag caccgttagc ttcttcgttg aaggcattcg
     8521 catatggatc gatattgata taaaaaaatt gctttttgac taacccgctg atcatgtcag
     8581 ctaattcctc atcttctttt gcaatcacta aataaggacg aacttgtgca gtagagtctc
     8641 ttaaccacat cgctggaatg tctcccgtca ataaaaaggt ggttccgtca ttttgctttt
     8701 tgactgttgt caacagagta ttggcaaatg ctgctgtaaa attctttgcc cagtccgcat
     8761 ggatatcccc acatttttgt gtgatatctt ccataaattt ctgcaccgat tttggtatat
     8821 ctttataagc cactaataaa accttctttc tatttgatat atctttaaag attatacttc
     8881 atacagccaa ttatgtctac gctttcaaaa aatatctttt tatttacttt ttcatccatt
     8941 atggtattat ttgaatatga tatatcatta agaggtgttt atatgaatca accattgtac
     9001 cagacaatat ttaatgattt gcaaacggct attttaactg gtgaattacc tgtagacagc
     9061 caactaccaa ctgaaaaaga actttcacgt acctataaag taagcagaat cacttccaaa
     9121 cgtgcattga ctgaattaga gcaattagga cttatttatc gcgttcgcgg gaaaggcagt
     9181 ttcgttaaat ccgcttctcc ggaacagatt cttgcttctc ctacaaaaac acgacggatt
     9241 ctgtttcttc ttccatatgt cgctgatttg tctgttggtg attttagcaa gggattgaac
     9301 cctgtgatgc aagataaaca gattgatgtc atgatgacga cgcttgactt tctacaaaat
     9361 aaaacagcaa aagagctaat caatgaattt gacggattga tttattatgc tcttgataca
     9421 gaacaacatc ttgacttact ttttgagctt tcattgaaag aattccctgt cgtcatatta
     9481 gataaaaaaa tctatgagtt accttttcct accgttcttt ccgataattt ccaaggcggt
     9541 gcgcttgctg cctcttattt gatcgaacag ggacattcaa aaatcgctta tttgtttggt
     9601 acgcagactc atccacaatc cgttcgacag cgttaccttg gttaccttga agcattggac
     9661 aaagctggtt tgactttcca cacttcactg gatgacaaaa gcgcgacatt agatgaattg
     9721 ctttcttata tcactaagta tgaagtaact gcttttgttt gtgaaaacga tgttgtcgct
     9781 atccagacca tgaatctctt gagacagcat ggttatcata tccctgaaga tttttcaatt
     9841 atcggatttg atgatatcca agcagcagct cttgttgacc cgccactgac aaccatcgca
     9901 caagattttg aacagttagg taagcttgcg ggagatgctt tggtcacttg gttagagaca
     9961 aaagatcgac catccgatag taaatttcca gtttcattca tcaaaagaca atcaacaaag
    10021 gagaaataaa atgaatatcg gtcaaataga tactagacat gcaacagcga atcagccaaa
    10081 tttctctaat gggaactgcc agccttatac tggcgttcct tttgggatga attattttgc
    10141 accgcaaact acggatcaaa acggcagctg gtggtttcat cctgatgacc gcgtttttca
    10201 aggctaccgt cttacccatc aaccaagtcc ttggatgggt gatttcagtc atatgctgtt
    10261 tacgccgatc aatggaaaac tacaggagaa cacccttttc catgctcaaa gttcttaccg
    10321 cccagaagaa agtatatttt gcccgactca tctgtcgatc tgtcaattac gttatggcat
    10381 ctgttctaca ttgataccta gcatgtatgg cggaatctta tctattgatt acagcaaaaa
    10441 tgacagcggt ttacttttat cctttccggg acgccatcag ctctttgtta tagatcctta
    10501 cacagtggaa ggaaaaatca cacattttgc tggttcagaa gatcctgatt tttcttttta
    10561 ttttatattg agatttgaat atcctttaac aattgatgag ttagctgttt ccaacaaaaa
    10621 tagcgactcg atcccgttgt attttgaaca gacaaaaaaa caaactatcc gatttgggac
    10681 atcttttatc agctcggaac aagcagctta taacctggaa ctagaacaaa accaaagcaa
    10741 agagacttac cttgctcaca gtcaagaaca atggcagtcc tattttgatc gtatacagct
    10801 cacacaccat gatcaacaac aattgcggac gttttatcat aatttatatc gggtgttttt
    10861 atttccacaa acattttatg aaattgatcc tagtgggcaa aaaatccact atgatacgac
    10921 tagtcgatct ataaaacctg gtgttctcta tacaaataat gggttttggg atacgtataa
    10981 aacggtttat cctctttatt ctttgattgc tgtagaaaaa tacgaagaaa tgctggaagg
    11041 ttttttgaac agctatagag aaagtggcta tttgccaaaa tggctgtctc ctgatgaacg
    11101 tggacttatg cctgggacat tgatcgatgc ggtcattgca gatgctgctg taaaagatat
    11161 ccgacgagac ttgatgcctg aatttctaac ggcaatggaa aaaggtgcta cggttcaaag
    11221 caaacatcca aattatggac gccaaggaac agaagattac ttgacatatg gctatgttcc
    11281 taatcactac cacgaatcag tcaatcacac attagactat gcttatagcg atttttgtat
    11341 tagccaagtt gccaaggtac tagaagaaga ggaaatgacg aagaaatatc agcagcaatc
    11401 attgaactat caaaatatct ttgactatga gtcaggattt atgcgttcaa aagataaaga
    11461 tggcaagttc agagaaccat tttcagctaa tcgttgggga caagattatg cagaaggaag
    11521 tgcttggcaa agcagctttg ctgtttatca cgatttttca ggactgatcg gaaaatatgg
    11581 tggcagtgag agatataaag aaaaactcat tgagctgtgc aatcagcccc caacgtttga
    11641 cccaggagga tatggttttg agatccacga aatgagtgag atggccgcga tagaatttgg
    11701 acaattagct atttcaaacc agccaagttt tcactatcct tacttgttca gttatatagg
    11761 acaacctgaa atggcacagc cactgcttaa acagttgatg actcagacct tcgacgattc
    11821 tccaatgggt taccctggag atgaggacaa tggaagtatg gccgcttggt atatcttcaa
    11881 tagtctggga ttttatcctg tcacgcctgg aaccggtgaa tatgttatcg gtatgccatt
    11941 aattgacaag gctgttttgc ttttgtcaaa tggaaaggaa ttgacgattg aagcaacgcc
    12001 taatcggccg cagcaacaat ttatccataa aatcaaacgg atggacaaag aatatacgaa
    12061 attatatttt acccatcaag atttgctgaa aggcggcacg atctcttacc aattagggat
    12121 catccctcac cctaagaaat atcaagcaaa agatttgcct ttttccttat caaactataa
    12181 ttaacaagag gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa
    12241 tagatcctca tatttttcgt ttggttggtc ttattaccgc agatgcaagc ttttgaacac
    12301 cgtttattga gaggttgtga aatcagcgtt ctgacctgag tattagagag acaaatggca
    12361 aaatagttcc tcatattttg ccattttttg agctaatacc gagggtcagc tgattgaaca
    12421 ccgtttagtg gagaggtcgt gaaaaagctg tttagtttcg agtattaaga aaaattttgt
    12481 gaaaatggct ttccacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
    12541 actccgttta tcgagaggtc atgaaaaagc tgccttgcct caagtaataa gaacagccca
    12601 aaattagatt tctagacaat tttttataag ttatttcatt acattggctt tttcttttat
    12661 gattctccac tgttttctcg gtactcttta ggggatctgc cgacgatttt cttgaataat
    12721 ttagagaaat acgtggtgtt gttgtagcca atctcacttg ctatctcatt gatgttttga
    12781 ttactgtgca aaagcagttg tttcgctttt tccatacgta aatggttcaa ataagctgaa
    12841 aaactttttt tcgtttcttt tttgaacagc tgtcctagat acatgacatt taaatgcagg
    12901 ttttctgaca cttctttgag tgtaagtggt tcttgataat gctggtgcag gattacgatt
    12961 actttttcca cattgtcact gtactttttc tgtctctgat gctttttgat gaatgacaga
    13021 agtagctgct gaagttcttg caccgttttt gcctgatgga ttttttcgat tccttgtaaa
    13081 tattcctcat cttccagatc aatcaattcc cgttgaatct ccataaacag taaaaaactg
    13141 gaatatcgta tatcttccgg catcatggct gctaactgaa actgttcaaa aaaatcagtg
    13201 atcatttttt gagcgacttc caattgttta ttttgcagca cgcgtctgaa acttgaaaaa
    13261 tcgatggttt gatcttttgt ttctgcttgt actgcgtaaa aaacatgctc gtctttatct
    13321 ccataaaatt gatgcaactg tagattgtct ttcgcttgtt ggaatgtttc agggattttg
    13381 tccaaagaat ctgtctcttc cccgatactg atcagccatt cctgatcagg aaagttttct
    13441 tgaacaaaac aacagaagcc atcaacttca tctgcctcca acaacaacgt gatcaataca
    13501 cggtctccgt aatttcgttg atagtaaaag atttgttgcc gttcttgcaa ccatctattg
    13561 ataagagtat catgagttct tgccagttga atcaatacta tcctatgctg cttctcactt
    13621 atttgtccta ttatttcttc ttcacttatt tcatccaatt cttcattcaa ccattgactg
    13681 aataacagtt cttgatagat ttcttgttga tttttttgtt cattttgctg atctaaccgt
    13741 gttttgattt tcttgaggct ttcaaccaat tcgaacttat tcacgggttt catcaaataa
    13801 ttcacggcgc ctaattgcat acctcctttc aaataatcga attcttggta tcctgataaa
    13861 atcataaatt caaaattatg acgttctttt tgagccgctt caataaattc cagtccattt
    13921 atttctggca ttgtcacatc cgttactact aaatcaatct gtcgctgttc aagtacagct
    13981 agtccttctt tggcattctc tgctgttgct actatttgaa agcctaaact ttgccaatca
    14041 attatctttt ttaacccatt caaaatcatg tattcatcat ctaccaataa cacttgatac
    14101 acgctcatcc ctccttgaat gtaatagaaa cggtcagtcc acctgtctca ttattctcaa
    14161 gcgacatata ataactgctg ccgaagctgg cacgtaaacg ttcattcacg ttttgcagac
    14221 cgatagaacc gtgaagttcc actttaggat gagacagttt ttcttcaatc tgttttagcc
    14281 gtttttcagt catccctttt ccattatctt tgatgatgat ccttacccgt ttgccttcct
    14341 gcaagacttt gacacttagc gcattatcga atcgagtaaa atcaataccg tgtttaaaat
    14401 aattttcaac taatggctgg atcacaaatt taggtacttc gatcttttcc aaatcaggat
    14461 cgatcataaa atgataggct actcgattag gataacgcat ttgatacaaa tagacatatt
    14521 tttcgcaaaa gtccagttct tcttttaaag tgatcgtttt ctcttgattc gtattgtttc
    14581 gaagcaaagc ggaaaacgca tacacaacat ctgctaattc ttcacttcct tcgctgattg
    14641 catacattcg gatatattct aatgtattat ataaaaagtg tggattgatc tgtgcttgaa
    14701 gcgcccgcat atgtgcgtct tgctgtttga tttctaattt gtagatgtct tctacaaact
    14761 gattgatgct atccaacatt tcgttgatac cgattgaaag ttccttcagt tcatattccg
    14821 tgtttgttat gtcgatccgc gtctctaaat tccctgctgc tgtttgatcc atggcctgca
    14881 tgacgataga tacttgctgt gaatatcgtt tgaatgtttt ataaagaaag ataagcagta
    14941 tattgtctaa aataatgata ccaatgatca gcggggcaag attttttccc gtttcgattt
    15001 ttacactttt tttatctagt acagctaata tacgataatc atcagacagt tcttggtgtt
    15061 gaacaaaatt gtttttttcc agatcgtcaa tcggcaattg tgaatttttc ttcagactct
    15121 ctgtgatgat catttcttgt ctttcaaacg tttctttagc tattttatta tcatggaatg
    15181 tgtatagtcg atttgtcgtt ccagaaatca tgtataaaga gagtccattg aatgacgtta
    15241 gattttctaa aatcatatct agttctttct tttgaaaacc aataaaaata cttcctgact
    15301 cagctcctcc ttgtgtgata ggcaacttga tataaaaggc gtctagtaat tttggtgtcc
    15361 ccttttttat ttccccacct ttgttttctc tagaagattc ataatactcc ggtagttgat
    15421 tcaacacgat atatatcgca cttagttctt cataatttgc gtagagatct ttgatttgat
    15481 ttgggaaaga aactaattca tcggtactgt tttgctgttc gtatacatac atcagatact
    15541 gatctatggg ttttgttaag taggtcgtca cattttcgat cttttcggaa ttccctacaa
    15601 gctggttttt gatgatccta ctcaaggttg ttttatcatt cagcattctg cttgtgcttt
    15661 gaacggcatc acttgtcatg ttttctgcct gtttttgatt ttgttcccaa gtatggatac
    15721 taaaaataac ggtagcagtc atcgttacac cgatcatgat aaccgcgtat ttccgcaaca
    15781 gacgatttag taaggttcct tttgaaaata gaatccgaaa cttattcata actttccaac
    15841 tgtttatcta attcttcacg ccaatgtgtt gtcatcgtac cattccaaac cgtcaaaaga
    15901 ccaatgacac caaacaggat caatcctttg aattgccaag tcacgcctaa aattataccg
    15961 ctaccaataa ttatttttag aaaagacgaa aagctggaaa aaaccgaaat aaaacttagc
    16021 tttagcagat taggaacaga gatcgtatat tcactatcta aaatcatact gtattgataa
    16081 gtagcaaa
//
LOCUS       DACDIP010000057        15963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDIP010000057 DACDIP010000000
VERSION     DACDIP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.057"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..450)
                     /locus_tag="IV257_002086"
     CDS             complement(<1..450)
                     /locus_tag="IV257_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8815185.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            673..1464
                     /locus_tag="IV257_002087"
     CDS             673..1464
                     /locus_tag="IV257_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8815186.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1430..2383
                     /locus_tag="IV257_002088"
     CDS             1430..2383
                     /locus_tag="IV257_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP8815187.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2781..3755)
                     /locus_tag="IV257_002089"
     CDS             complement(2781..3755)
                     /locus_tag="IV257_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8815188.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3870..4304
                     /locus_tag="IV257_002090"
     CDS             3870..4304
                     /locus_tag="IV257_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8815189.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4429..5415)
                     /locus_tag="IV257_002091"
     CDS             complement(4429..5415)
                     /locus_tag="IV257_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP8815190.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5843..6265
                     /gene="arsC"
                     /locus_tag="IV257_002092"
     CDS             5843..6265
                     /gene="arsC"
                     /locus_tag="IV257_002092"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP8815191.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6545..8467)
                     /locus_tag="IV257_002093"
     CDS             complement(6545..8467)
                     /locus_tag="IV257_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8815192.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8554..10494)
                     /locus_tag="IV257_002094"
     CDS             complement(8554..10494)
                     /locus_tag="IV257_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8815193.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(10782..11522)
                     /locus_tag="IV257_002095"
     CDS             complement(10782..11522)
                     /locus_tag="IV257_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP8815194.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(11535..12626)
                     /locus_tag="IV257_002096"
     CDS             complement(11535..12626)
                     /locus_tag="IV257_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP8815195.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            complement(12616..13722)
                     /locus_tag="IV257_002097"
     CDS             complement(12616..13722)
                     /locus_tag="IV257_002097"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP8815196.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            complement(13741..14418)
                     /locus_tag="IV257_002098"
     CDS             complement(13741..14418)
                     /locus_tag="IV257_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP8815197.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            complement(14429..15208)
                     /locus_tag="IV257_002099"
     CDS             complement(14429..15208)
                     /locus_tag="IV257_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP8815198.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            complement(15237..15587)
                     /locus_tag="IV257_002100"
     CDS             complement(15237..15587)
                     /locus_tag="IV257_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP8815199.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            complement(15603..15959)
                     /locus_tag="IV257_002101"
     CDS             complement(15603..15959)
                     /locus_tag="IV257_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815200.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
BASE COUNT         4737 a         3344 c         2690 g         5192 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgcttcaaa agaaatttat tttgttaaat ttgatctact tgaaaataaa
      601 atcgtttatc tatataatta agaagaacaa agtcgaacaa aaattgactt ttatgttaaa
      661 ggagtaattc atatgctgaa aaaagaaaga caagaacaaa tcattacatt attagagaaa
      721 gatttttatt taactaattc agaaatcgct aacaaattag gcgtatcaga aatgaccata
      781 cgccgagatg tcactgaatt agcaaaaaaa aataaagtaa aaaagctata tggtggcgtt
      841 gaacgaaagg aaccttataa tcgagaacta tcgactcaag aaaagattga tacgaatgta
      901 gccgaaaaaa aatatattgg tcaagtcatg aacagtatga ttcccgataa ttctactatt
      961 tatgtcggtg ctggaacaac gatcctatat gcacttcctt tcattcaaaa gaaaaatcta
     1021 tttgtaatta caaacagttt gattgctttc aactatttaa agaatcatac aagttatcgg
     1081 attttactta ctggtggcga attttctcct attacagaag agttcattgg ggaagttgca
     1141 gaaagatctt ttgaaagttt gaatgtcgat atctctttcg ctgcaacaaa cggcatattt
     1201 gataacaatg tgacaaccgc tcaatttatt gagggaaata ttcagaacgc agcgctcaat
     1261 gcagcaaaaa tcaaatgcat tgttgcagat tattcaaaat taaataaatc agacgtctat
     1321 acttttagaa aattaaccga ttttgattat ctaataaccg ataatcattt atcagaacag
     1381 gatttcaatc attattcaaa atacttaaca atattaaagg agcccaaaaa tgatactgac
     1441 aatcacgatg aacccatcca ttgattccat ttattttaca aaacactttg aattaggtac
     1501 gatgacacgc tttgattctc cggtacgcta tgtaggcggc aagggaatca attgcggacg
     1561 agcttctgct ctactaggtt catcagttat tttaacaggg tttttgggcg gttattttgg
     1621 cagtatgatt caagatatgc tgaaaaatga aaatttgttt gatttgcatt ttttacctat
     1681 tgcagaagaa tcaagaggtg ccgtcacgat catgcatgat gatgaaacgc agacagagct
     1741 agtggaagct ggtcctgaaa tagcagatac tactatcaaa aaattaatga atgatatcaa
     1801 actaatcagt aaaaataatt acctttcagt cattacaata aatggttctg ttaacaatcc
     1861 agatcccttt ttgtatgcta aattattaca atatatcaaa gaacaaatag atccttccac
     1921 ctatatatta atggatatat ctggacaaca tctccgtgaa gtcttagcaa gtaagtataa
     1981 accgatgttc atcaaaccta atatccatga gttttcagat atcatccatc aaaaaattac
     2041 tcaaaaaagc gacataaaac accatctaaa aacaaatcca ctatttaaag atatcgatgt
     2101 tatcatgatt tcttgcggct cgtatggtgc agtagtcaaa gtaaaagaag aattttggaa
     2161 tgttgagatc cctaagattc ctatcattaa tacaacaggc tctggggatg ccactgttgg
     2221 aggttttgct cattcattag aacagaatta tcctttaaaa gaatgtgtaa ggtacgcaat
     2281 ggcttgcggc atgtcgaatg cacagcaatc agaagtcggg aaagttattc cagctgatgt
     2341 aaaaaattta gcaaaaagca ttaaaatcac agaaatgaaa taaatagtac aaagtaaata
     2401 attgtttata tgaaatgaga cacaaagaga ctgtgacaaa aaacgtgtgt accgaccccc
     2461 aaaagttaga ttttttggtc taacttttgg gagtcgatac attgtcacag tccttttttc
     2521 gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg
     2581 agaagctatt ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacgacct
     2641 cttggtataa acggtgttca aaagcttgca tctacgctaa tgagtccaac caagtcaaaa
     2701 atgaggagct tttttggctg tccttattac ttgatgcagg gcagtttttt cataatctct
     2761 tttagattaa atccttttgg ttacacaagg ttctcttcaa agaatgttgc cattttttca
     2821 aatggaatga tgtctgtctg gtcgtataga tctgtatggg atgctcctgg aatgatcatg
     2881 agttccttgt tgtcaccttt tagttctttg aaagcgtcct ccccaaagta acgtgaatga
     2941 gctttttccc cgtgaatgac taagactgca tgacggattt ctggaatata atgcaatagt
     3001 tttgcattca taagtgacaa ccctgatgtc ctattccaac catcattgga gtttaaagaa
     3061 cggacatgat atccgcggtc tgttttataa tacgcatgat agtctttgac aaattgagga
     3121 gcatcttcag gcaacggatc aaccacacct ccagcacgtg cgtagtcccc ttgtttgaaa
     3181 tcaatgatgc gttgtgcatt taattcttca cgcatttcat atcgggcatt ttcatccata
     3241 acatcgaagt agccatttgc tgttactcga gtcatgtcat acattgtgga aacaactgtt
     3301 gctttgaccc gcgtatcgat agctgctgca ttcaatgcca tacctcccca tccacaaata
     3361 ccaatgattc cgattctctc agggtttaca tttttttgta cagataaaaa gtctactgcc
     3421 gcttggaaat cttccgtatt gatatcaggc gaagcaacat atcgaggtac tcctccgctt
     3481 tctcctgtaa acgatggatc aaacgcaatc gttaaaaagc ctctttcagc catcgtctgc
     3541 gcatatagac cagacgattg ttccttcact gcaccaaatg gcccgctcac agcgattgcc
     3601 ggcaatttct tttcacttac ttcttttggt tcatataaat cagcgactaa tgtgatacca
     3661 aaacgattcg ggaagctcac ctttcgatga ttgacttgat tgctttttgg aaatactttg
     3721 tcccattctt gtgtgatgtt taattcttta ttcatattct agactctcct ttgatcaaaa
     3781 ttgacatttg tttttgaatg tattattatg ataaaactta aacttgactt taggtcaagg
     3841 taaaaatttt attttaggag gattttttta tgtattccat tggacaagtg tctgaaatgt
     3901 ttggattgcc tgtttctact ttacgttatt acgacaaaga gggattattt cctgatatcc
     3961 agcgtgtttc tggtattaga aaattttcag agaatgagtt agaggcttta agagtcattg
     4021 actgtttgaa aaaatctggt ttagaaataa aagacattaa gcaattcatg gaatggagca
     4081 aagaaggcag taagacattt gaggttcgta aagaactatt tgaaaaacaa aaagaagtag
     4141 tagaaaaaga aatagcaaaa ttagaaagag tactcgctat gctaaactac aaaagctggt
     4201 actatgaaga agcaataaaa gccggcaatg aagaagccgt attgacaatg attcctgatg
     4261 atttacccca acatgtaaaa gaagcttatg tacattctca ttgaacaaac ggataactca
     4321 ctctccttct ctatgaactt catgaaaaca tgaataaaca cctctaactg attgttagaa
     4381 gtgtttattt tttcactcaa gtatcctgca cttacaaaaa tatccttttt accgccatcc
     4441 taattctgga gcgatatctt ttacgattgt ttcaagcaag tgcatgttat attctacccc
     4501 caaagtatta ggaatcgtga caagtactgt gtctgcttct ttgattgctt catcttcagc
     4561 tagctccttg attaattgat caggttcacc agcaaaagtt tttccaaaaa tcgttgggtt
     4621 tcggtgataa gcaagatagc cgacagcatc ttgttgcgga ctaccgtctt gtccaaataa
     4681 gcgtcgatcc aaatcagtgg tgattggctg aatcgagcga gtaactaatg ttcttggttc
     4741 aaaatcatga cctgcttgct tccacgcttc tttatacgca cgtaattggt ttgcttgttg
     4801 gatatggaat ggctcgtggg attcgtaatt gactaatgta gatgattgca gattcattcc
     4861 atgttgtgct gcccagattg ccgtttgctg cgaaccagct ccccaccaga ttctttgtcg
     4921 taatccttct gaataaggtt ctatccgtaa ttttcctgat ccttgtggga acattggttg
     4981 aggatttggt tcttcaaatg gttttccttc aagtaattcc aagaattcaa gggtgcgttc
     5041 ccgtgttacc tctttgattt cttcttcact gtaatcataa ccaaagtatt tccagccatc
     5101 tagaacttgt tcaggtgaac cacgccccac tcctagttgc actcttcctt gcgtgatgat
     5161 atctgtcaat ccggcatttt ctgccatata ataagggttt tcatatcgca tatcaataag
     5221 tcctgtaccg atttcaatct tacttgtctt tgcgccgatt gccgctagca aaggaaacgg
     5281 cgacccaatc tgaggggcaa aatgatgtac gcggaaaaac gctccgtcca ctccgatttt
     5341 ttctgcttcc accgctaaat caatcgattg caaataagca tcttgagctg atttcacaag
     5401 cgatccttgc tccatccaat gtccaaagct taaaaagccg atttttttca aggtaatgac
     5461 ctcctttaaa cctacaaaaa tggaaggttt tttattttta atatgaactg attctactac
     5521 tttttttctt aaaagacgaa taattgttct cgcctcacaa tcaagaaatg tatcggctta
     5581 ctacacccct actttcttca tcatctgccg tgtcttaatc tcttttaaca gtctgttaca
     5641 tcgttgattt ttgactcaaa atgattgttt ctaacatgta tttatggtaa aaaagacaag
     5701 attagttctt tgtacacatc gatttatacg ctgaacaaaa agaaatatat aaactaagaa
     5761 acccattgtt ttttcgctcc atcacataga gtttacttta gataaacagt gacatttact
     5821 aatccataac ggaggaaatc aaatgaaaaa aatctatttc ttatgtacgg gcaattcatg
     5881 ccgcagtcaa atagcagaag gctacgcgcg taaatacttg tctccttcaa tgtttgagat
     5941 aagaagtgcg gggatcgaga cacatggctt gaatccaaga gctataaaaa tcatggcaga
     6001 agacggcatc gatatttcca aacagacgtc tgatttgatt gatagagatt actttactca
     6061 ggcagacttg atcattacac tttgtggtga tgccaaagac aagtgccctg ccgttccaaa
     6121 caatcacgaa caccttcact gggacctaaa agatcctgca caagctgtcg ggagcgaaga
     6181 agaaatatta acggaattca gaaaagtcag agaaggaatc aaaagaaaag ttttagactt
     6241 aaatcaacgt acatcgatcg tataaagaag tattcaaaat gatttttcaa gcactaagaa
     6301 acaatgttaa aaaaatagct ttccatattt tacaaaattg tgccttattg ccgacaaatt
     6361 gtctcttgaa caccgtttac cagtagtcaa gacatcgtaa gcaactataa aaaattataa
     6421 gaaactcaaa ataaaaggat gtgccacaac tttggccaca tcctcttttt ttgtatttag
     6481 ttttctactt ccaaataaat agatagaatt attataaata taaaggcagt tttttaatcg
     6541 ctacttattc ataactagat aacaacgtta agatttctag cggatgattg gattgcatca
     6601 actgagccat ttgttctggc tgcttttcta gatagtgaat aacagaaaat atttttttga
     6661 actctgcatg tttcaaatca tttttcttta ttgctaatag cagtacgata cgtatctgtt
     6721 ccccgtgcca taggactggt tctttcattg tagcaataga aatcatcgaa tgcttcacat
     6781 acagcggatt accgtgaggg attgttacaa atggtgctac tctagtactg gagtgactgt
     6841 ctctttccaa gacagaatcg atgaattccg gctccacata atgatgttcc actaagacgt
     6901 ttcccataaa gtttaaaata tcggctgggt ctgtccgttc aagttgagga aagatcagaa
     6961 atggatgagt aaagcttgct agatcaaatg tttttttctt cttgataggc acatgctcca
     7021 gcatatattt tgtcaaatag gctagatcag tttctgtgat cattggtgag atctcgatta
     7081 ctggaatgct tatatgttta aatggtaacg tagccaagat aaaatcaaca gagggaaaat
     7141 cttgtctctt taattctggc ttatattcaa ctagatccat aatcgttaat tctggaagtt
     7201 ctctgttcag ccgtgcttcg agaaaagtag ccatagcttt tgaatattca ctaactaaaa
     7261 ccgtttgata tcttctactt ttctgatgtt tgtgtcgctc gatcgctgct tggaaatgga
     7321 tcgttaaata agctgtctcc tcttgtggaa cataaaaatt ttccttttca aaataatctt
     7381 ctaaaattaa ctgtaaaact aagaaaagtt gtgtgtacgt actaaacaca tttttcgtca
     7441 atggattaga aatctgcaaa ccataatgga gtcgaaaata agttgaagat aaatgagacc
     7501 ttaaattttg ttttagtagc tgatcttctt ttaatgaata gcctagaaac tgttccactt
     7561 gttcgatcaa atgatcagca agtgcgctag attccttttg aaaacgtgtg gctgcttgtg
     7621 aatttgtaaa aagattggcg atacgcaaag ccagataatt gatttcatca ggagaaaatt
     7681 caattggata gatggtggct agttttgcta atatttgact acttaacttt tgtgcttggg
     7741 tgtgttccac gacttcgttt tcgtctttac ttaatttcac tttctggtac tgtttcattc
     7801 gctcgagcat aaagtagatg tgaatctcca cactagccag tccattcggc tgttctaaat
     7861 ggttttcata aaaaacatct tgtaatgtat gttggataac ttttaatgta tctttagcga
     7921 agaattcttt caacgacgga ctttgtggat cttctttctg catatttcgt aatgtcttaa
     7981 caagcaactg tcttttttct tgttcatcgc ctttaagaaa agttcctagt tttgttttgg
     8041 atactagctg taaatggtga tactcaaaaa agctttgcaa ctctgtcaat tctttacgaa
     8101 tcgtacgttt tccaacatat agtttttctg aaagttcttc aagactaaat ccgtcttttg
     8161 ctactagcag atggaaaatc aataactgac gaagctgttt tttatccatt tctgtttgag
     8221 tctcttcatt aagcgctcgt ttcagttgtt ccctttcgtg aggcagacca ttcaacaaga
     8281 tacctgtacc tttttttctt tcaaacatta aaggcagatg tttgtctctc aaccaagttt
     8341 caatggtatt caaatcgcta tgaatcgttc tttctgacac atggaaatgc tctgctagct
     8401 tttttgttgt tatataatta ttgttctcac ttaattgata gatcaaagat tgctgacgct
     8461 gattcataat gtgcagaaac tcccttctaa ataaaaaatc gatcatgata acacatctat
     8521 tatgtctcat cacggtcgat ttttcatctt atgttacatt tcgcttaata attggtgaac
     8581 tgttgtttta tctggcgctt ccatcagtgc ttgacggaag tcatcatcca tcaatttacg
     8641 tgatagacgt tgcagtattt tcaagtgcat atcaccttgg ctgttttcag gtacagcaat
     8701 cataaagatc atatgtgcag gttttccatc taaagaatgc caatctaccc cagattgact
     8761 tctggcaaat gccactgttg gtttaagaac ataagctgat tttccatgtg gaatagcaat
     8821 ccccataccg attcccgttg tactttcatg ttctctatca tagatgactt ctttaaatct
     8881 ggttttatct gtgatcgccg ctgttttttc taaacgttgg actaaatctt cgattactcc
     8941 gtcttttgtt gttgctgttt cacctaaaac gattgcttca tctgacataa tatccgtgat
     9001 agaatcaaaa gtttcagttt tcgtataaac atcacttgtt tcagtctctg atagatctgc
     9061 tactgtcaca ccatttgctg cagcaaccgc agtttgttgt aatgtcggtt taaggattct
     9121 taacattagc acggttacag caataccagc aatcactgct acaaaaaaca tggctacgtg
     9181 atccactgct cctaaaaccg ctacgatcgg accgccatga gctacatgat ctgtcactcc
     9241 tgaaagcatc gcgatcacac ttcctgtcat tgccccgatt acaatgcttg gaatcacccg
     9301 tagtggatct gttgaagcaa acggaatagc gccttctgta atcccaaaca gtcccattgc
     9361 taaggacgcc ttaccagctt ctcgttctgt tttcgtaaat ttggttttta aaatcaatga
     9421 tgccagcccc aatgctaatg gagggataca gatagctaca gcaattggcc ccataatgga
     9481 ataattgcct tctgcaatca agccagatcc gaacaaaaag gctactttat tgaacggccc
     9541 ccccatatca aaagcaatca tcgctcctaa gatcatagct agaacaattt cacttcctcc
     9601 ttgcatccct gctaaaaagt gagtcaatga actaaataga agagctactg gttctccaat
     9661 aacatagata aacacaaaac caactgccaa tgatcctaaa attgggataa caataattgg
     9721 catgatcgat tgtaaagctt tcggtactcg cactttcttg attcctaaag caatgaatcc
     9781 tgctaaaaat ccagtgatga tcccgcctaa gaatcccgca ttcgcatcac taccatataa
     9841 tgctccgttt gccgctaaca tcccaccaac aaatccagga acaagtcctg gacgatctgc
     9901 aatactttgc gcaataaatg cagatagaat tggaaccatc aaccccatag cgatacttcc
     9961 gatactattc atcgttgccc aaatcgttcc ttctggtatc ttgatccctt caggtgtagc
    10021 tgttcctcct aatgttaaag aaatcgcgat caataaccct cctgtaacta cgaaaggtac
    10081 catgtacgaa acgccattca tcaaactctt atagattttt ttacctaaat tttctgattc
    10141 acttttattt tcggaagaaa taattgcctc cgtactttta tagactggcg cttttcctgc
    10201 taaaatatct ctcaccaact catctgcatg gtggattcct tcttgcacac ctacggttat
    10261 caaaggttta ccgccgaatc gtgatttatc tattttagta tctgctgcga tgattacacc
    10321 agctgcttct tcgatatctc ttgcagtcaa ttcattttca ataccgatgg aaccatgtgt
    10381 ttcgactttt acctcggcgc caactgcttt ccccgctttc tctaagtttt ctgctgccat
    10441 gtatgtatgg gcaataccaa ctggacagga tgtgattgca ataatttttt tcattttcct
    10501 accgcctttc tacatttata gtatataaaa acggtttctt tttattaagc acaaaatgat
    10561 tccgtaataa ctgaaattct tggaataaat agaaatttcg ctaaaaaaat tgatatacga
    10621 tttattatgt tgaataaaat aatattggaa aatcttacat ttctgtactt ctgcttacgt
    10681 acatctaaat tattatctac attaacaatt tccgtaatta attataaaaa gcttattcca
    10741 tttaatttca ttctggaata agcttcttat tttctaatga gttagtgaat aatttttttt
    10801 accatgctta atagtatctt tacgtctcct atacgcgtat ctcctgtttc tttatctata
    10861 attgaactat aaacatgagg aatcactttt tttacacctg catcgattgc aattttcaga
    10921 atttcttcaa agtttgaaag atcaattcct cctgttggct ctaaatagaa ttcatatttt
    10981 gcgcaagctt ccgcaactgc tttaaattct tccctatgtg ctaagccttt catcgggaaa
    11041 tacttgattg aagtacctcc catgtcttgc agcatttgaa tggcagtttc aataggcact
    11101 tcagctgctg gcgcttgtga acttaacgga cctgtagcaa tattgacata gccgatctta
    11161 cctgttggtg aaaccaatcc attgatgact gtatcatttt ggcccaataa agcacgagaa
    11221 gtccccacgc cagtaaatac ttgattgaca tgttgtggtt gtaaaacccc agataaacgc
    11281 gaaaccatct gactttggtt tggatctcca gcgcctaatc caactgacaa cgcattattg
    11341 gttgcgtctt ggtacctttt catatcttcg attgctgctt catcgctagc ataatttttc
    11401 gatagcacac ctagtacgac atgcccttcc gctgcttcat agcatgcttt tgcattttca
    11461 actgaatgag ctaatacatt taaacataca cgatttttat aataatttgg gataactgac
    11521 aatttaaaag cctcctacat gatttctctc aatcttttta taattttttc catttcgttt
    11581 tggctgacgg aacgaatgtc gaactcaatg attccgttat ttgcttgata ttctcgggtg
    11641 tagactgctg gatttcgtgc ttttaattcc tgaatgactt ctttcgcact tttatcgcca
    11701 ttaatcgtta cacttgctcg ataaatatct ctgccggcac catcttgaac tattttcgct
    11761 gaaaggtcag gaagttgatt gattgcgtta acaaaaggcg ctaatcgttg cttcatggag
    11821 tcaccggatt cgctcccatt ttttaaataa tcttctagtg cctgggtaaa gcctaaaata
    11881 ttatctttac cgattttcat tgctcggcca atacctttag actgtagccg aatccattga
    11941 atatacttct tttttcctat gaccagtccg gcactcggac cttcgattgc ttttgcccca
    12001 gagtaaatca ctaagtcaac gcccatttcg ctatatttga ataaatcttc ttccgcagct
    12061 gcatcaacga tcaatggcaa acgatgagct ttggcaactt ctaccatttc tgccaccgtc
    12121 agcatacttt tctgaactgc atgatgactt ttaatatata gaagcgcagc agttttttcc
    12181 gtaatcatca tggaaacatg ttccggtgta cacatatttg catagccagc ttctatgact
    12241 tttcctccac cttgagcaac cattacttca accggtgtcc cataatctac attatgtccc
    12301 ttaggtatga tgatttcccg tctagcgatt ttttctgtat agggatgata gacatggtac
    12361 atgtcgccct gtccgattaa agcagcaaca gattgagcaa tccctgctga agcagaagaa
    12421 acgatttgcg catcttccac tttcagtaat tgagaaagat aagcgccggt tttgatggca
    12481 agatcactca tctcaaaaaa atgctcgcct ccaaatcgtt gtgctgctag gacactttca
    12541 gataccttag aaacccctag gatcgtcatt tttcccgatg cattaatgac ttcttttaaa
    12601 tcaaactttc catagctaat tgtcatatat gacgcctcca ataattgttt taatcggtaa
    12661 aatttgttct ttagctatac gtgtaaatcc gttggaatct ttcaattcct tttcattttc
    12721 aacaatctca aatatcgtca aatctgcatc ataattgaca gctaactggc ccttattctt
    12781 caaatgaaaa ttccgtgcag gaactgctgt aactttttca atgatttctt cccaagaata
    12841 gccgactacc ctcaattttt ctaaagtagt tgctagatca aaaactgggc catttgtacg
    12901 attgcggatg tagatatccg tactaataga ggtagctttc attcctgctt ttagtgcggt
    12961 ttctgccaca tggaaattaa agctatccgt cccatgtcca atatcaaaga cgacaccatt
    13021 attgtaagca tcccagacaa agtttttaat ttgatctgtc tttggatcta aaataccgtt
    13081 tggttttcca ttaaaacaat gggtcaatac atcgcctctt tccattcttg acaatacttc
    13141 ttctaactca ggaggtgcag aaccgatatg aaccatcaat ggcagatcat tgttttctgt
    13201 ctggattttt ttagctaatt ctaatggctt aatgccattt tcaccaacaa ctgtcttact
    13261 cattctcgct ttaatcccga caataaattc cggcagttct gctaacgcag tacttactag
    13321 atcttcttga atctttgtta gatcagcaag ttcatcttgt ttgacaatgc cccatttaga
    13381 gatattcatt agcgcataca catttgtctt gaccgatttc gtcaattgat aaaattcacg
    13441 gatgttttct gctcccgtag ttccagcatc aatgaccgtc gtcacacctt tttcgacccc
    13501 tatctgatca ggatagtcat aataaagatt catcttttca tagcagtgga catgatcatc
    13561 gatccaccca gcggataaat aatactttcc ctccaagtca actacttctt gcgccggctg
    13621 atttatttct gctgcaactg ctttaatcat gcctttttct atagcgacct ctattttaga
    13681 tccatcaact aaccttccat ttttaatcaa agtatcaaac atttaatgac cctctttcta
    13741 ttaatttact gccgggaaga ttgctcctaa aatcattgct cctaaaatag cacctccagc
    13801 aattggtttt ttccaagcat agaatcctag tgctcctaat gtagcgccaa tgccgattgg
    13861 aatagatgct gcacacgcgc ttaatataat taatggaccc aaaaaacgtc cagatgaatt
    13921 tcctgccccc atcatgacgt cagcaccaaa agtggaattt gattgattga tcgtaaattt
    13981 acgaatcaag acaatgatgc caccaacaat caaacctaga accaaaccag tcaataaaga
    14041 gagtgcaaaa ttatcgattg gtgccgctat tttcatactt aagagaattg ctggtacccc
    14101 aattccgatc ccagttagga tcgaaccgcc taagtccaag atcccaacta aagagccttc
    14161 taaaattctg gcaaataaga aactagcacc aaacgctgcc gctgcgccat aggatccgcc
    14221 atccagcccg gcttttaaca tcgccacgat cgaaacttca ttgaatgcac ccacaccata
    14281 catcacatac atgtgtgttc ctgcaaatac agcagcggac ataattccta cgattagcgg
    14341 aaaagaccag tctgcaaacc aaaagctctt ttgccgcttt gcttctaata cttttgcttg
    14401 ttcttctgtt gtctccatga ttctcctcct atttcacact aaagatgttg tgcaagttat
    14461 caagccaatt aggcacttga agctggaagc tcttgatcat ttccatatca aatccacgga
    14521 aaaatccact gaaaataaat aataaaacaa tagcgatcat catgctcttc gtgacgcgat
    14581 tccaaccgct ctcatcgacg cctttaccaa tcaagattcc tagaacaaca ccaggtaccg
    14641 catttcccat aatcaattgc gcgataccac caaaaagagt gccccaaaaa ccagttcgtt
    14701 tccctgcgtc taaagctgcc aaccaaaaaa taactggcat tactgtattg atcagaatcg
    14761 tcgctgctgg taccaaaacg gaaaccgccg tcacttgtaa cgcctctgga attgctgcag
    14821 ctgttgtgtt taagaaggct accaccactg ccccgataac cgctccaatg atccccattt
    14881 ttttcggatc atgcatggtc tgggtgacat ctttgttttt tgctagaagt gctgccgctg
    14941 cccaattggg aatgattcga tgcgtcacat cttgtgtgaa tgcaccagca ccaactgaag
    15001 aagcccatgc attaaagaaa aagcctaaac caaatgaaaa atgagaagct ggatcaccca
    15061 tgcaggcatt catttcgcct aatgttctaa aagctcctaa accttgtgta ttaggtgcat
    15121 gaaacattcg agctgccccg acagatgctg caaaaccaag taatcccccg ataatgaccg
    15181 atttgactag aatgattacg aaatccacgt tattctttcc cccttatgat tcaaaattag
    15241 atttttttag ttacaaatgg aatggcaatc ccatttggat cttcagtcat ttgctggctg
    15301 aacgatatgt ctgccaattt tatttctgtt acagccacag taacagagag ttcgacttca
    15361 tagctaatac gttgccgtgg gaagaagaaa aatagaaagc gttctgtgta tttgttttcg
    15421 accgccttta aaatatccac attgactggt tcgattcgca ataatacgtt tgattgttcc
    15481 attattttgt tttgaatttc tgctaacgct gatgcaaaag catgttgctt tgtttcccct
    15541 ttaccggaga cgataacttt ttgctgaatt gtcttttgaa cgctcatttc atgtccctct
    15601 ttctattttc catgcttttt gataaattct tctgtcagac gtttccctaa ttcctcttga
    15661 tccataaagc cgaaacctag aacagttttg ccatccttga tagctgtgat cccttcttca
    15721 atcgaacgca taccatgtcg ttctggatag ccatattttg ttgctgctgt taatgcacct
    15781 gcaccaccac taccacaaaa agagataccc atatctgcat gctcttgttg catcacatcg
    15841 cccaaacgca tatctgcccc cattcctggt acgacaactg cttttccacc agctgcttct
    15901 acccctttgg ccacattttg tcctttaccc atacggtctg caataactac tttaatcatg
    15961 att
//
LOCUS       DACDIP010000058        15292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDIP010000058 DACDIP010000000
VERSION     DACDIP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.058"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..186)
                     /locus_tag="IV257_002102"
     CDS             complement(<1..186)
                     /locus_tag="IV257_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815201.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(210..473)
                     /locus_tag="IV257_002103"
     CDS             complement(210..473)
                     /locus_tag="IV257_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815202.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            631..888
                     /locus_tag="IV257_002104"
     CDS             631..888
                     /locus_tag="IV257_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8815203.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(959..1150)
                     /locus_tag="IV257_002105"
     CDS             complement(959..1150)
                     /locus_tag="IV257_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815204.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1291..1611)
                     /locus_tag="IV257_002106"
     CDS             complement(1291..1611)
                     /locus_tag="IV257_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815205.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(1634..2068)
                     /locus_tag="IV257_002107"
     CDS             complement(1634..2068)
                     /locus_tag="IV257_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8815206.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            2403..3041
                     /locus_tag="IV257_002108"
     CDS             2403..3041
                     /locus_tag="IV257_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8815207.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(3365..3448)
                     /locus_tag="IV257_002109"
     tRNA            complement(3365..3448)
                     /locus_tag="IV257_002109"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3412..3414),aa:Leu,seq:cag)
     gene            3976..4476
                     /locus_tag="IV257_002110"
     CDS             3976..4476
                     /locus_tag="IV257_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8815208.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(4666..5010)
                     /locus_tag="IV257_002111"
     CDS             complement(4666..5010)
                     /locus_tag="IV257_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8815209.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(5164..6084)
                     /locus_tag="IV257_002112"
     CDS             complement(5164..6084)
                     /locus_tag="IV257_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8815210.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            6271..6762
                     /locus_tag="IV257_002113"
     CDS             6271..6762
                     /locus_tag="IV257_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8815211.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(6786..9161)
                     /locus_tag="IV257_002114"
     CDS             complement(6786..9161)
                     /locus_tag="IV257_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8815212.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            9487..10113
                     /locus_tag="IV257_002115"
     CDS             9487..10113
                     /locus_tag="IV257_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8815213.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            10136..10840
                     /locus_tag="IV257_002116"
     CDS             10136..10840
                     /locus_tag="IV257_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8815214.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            11032..11865
                     /locus_tag="IV257_002117"
     CDS             11032..11865
                     /locus_tag="IV257_002117"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8815215.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            12062..13990
                     /locus_tag="IV257_002118"
     CDS             12062..13990
                     /locus_tag="IV257_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8815216.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            14128..14583
                     /locus_tag="IV257_002119"
     CDS             14128..14583
                     /locus_tag="IV257_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8815217.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            15053..>15292
                     /locus_tag="IV257_002120"
     CDS             15053..>15292
                     /locus_tag="IV257_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815218.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDE"
BASE COUNT         4570 a         2927 c         2689 g         5106 t
ORIGIN      
        1 aaataaacgt gtcggatcgt aatgataagt cacattgccg ttcagtgagg cttttcccac
       61 cacatattta cgattctcca acttcagcca cgcttctcga aaagccatta attcttctct
      121 ttttacatca atcttttcga tttttccatc gatcaaatct tgaataagtt gttcatatgg
      181 attcattttt ttcctccatc tgctgtcaat caataagttg taaaaatagg ttcatatgct
      241 tttttggtat ctagatcttc tctgactttg actgtcgtat aaataatttt ttctactggg
      301 atactataat aacttccttc accattattg aatcggtgaa cgtctttctt tgctaaaata
      361 ccatttattt tattcgtcag tacacgttgt tcgacactgt tacttacaac atagtaaatc
      421 acccgaatat tattgatcaa atgcaaatcg atttctaaca ctacatgatc catgaaaatc
      481 ccactccttc tttcactttg attatagcaa gtttctcctc taatttcaaa atcgatacag
      541 attttaccgg ataaatccac aaaactgctt gcgccatttc gcacaaagtg ttattttgta
      601 tcatggactt tatttatcgg aggcaatagt atgatcgcga tcggtcaatt tgttttttat
      661 attccttttt ttattatgat aagcattctt ttctattaca taaaatggac aaaaaagaag
      721 ttttcagttt tactggcctc tttacctgct gtatatttta cttatcaaat tttttctttt
      781 aggcattggg aaacaacgtc tgtattaata acacacatca tcgaattgac gctttcagta
      841 atttttctta ttatttggat ctatttttta tataagaatc agaattgaat ccctcgcatg
      901 tttaaaaaga aaacgattaa agatgcagca aatttctgcc gatctttaat cgttttttct
      961 attctgttat accaaaaaaa tgtttgaatc ggtgaaggat cgttccttgt tttaattcta
     1021 taaattcttg tgcgatcggg tacttttctt ccaaattttc tttgttcttt tcgatcaggc
     1081 ttttcgcttc ttcatatttt ttatctgcac atagtgcttt tacttcctgt aatacttttc
     1141 ctgcttccat ttgcctcacc ctcttgccat tttattgctt taagttttat ttaagttata
     1201 cgcattattc tagtttcatc ccaaataacc ttttttcatt tttactcctc gaactaatca
     1261 gttttctaaa ggctgattgg ttcgtctttt ttatttttcc tccatttcat tctgctcttc
     1321 tgttaattta tctaatgctt tgattgatgc tgcggctctt gcataatcat agctgtataa
     1381 ttccataaat ttatcaggca gaacaaacca cgctggatga taaccgttag ctgtgacagg
     1441 gaatccttta tcttgttggt tatctccatc ccatctcaga gcaacacctt tttttccttt
     1501 ccactttact aaagcgatcg agtatccctt ctttgtttgt gtgccgtctt caataattgc
     1561 taaaatttct attttttctt ttggcgcacg gacttcttga ggacgaacca ttttctattc
     1621 ctcccttatc tcattacttc acgaccaaca cactgcaagg ggcatgtgat acgacataag
     1681 aagcggtcga tccaactaat gcttgttgta tagcaccttt tcctgtcgct cccataacga
     1741 tcaaatcaat attttcttgt ccagggatga cattggcaat caaatttctt ggatctccag
     1801 tttcaactat tgcgtggatc tcagcaatgc cgtactcttt ggcatcattg atttttttca
     1861 gcatggctac ttctgttctt tgtttttcat ctgcatagat ttttgaaaag gcataggcac
     1921 tatgggtcag ctcaactttg ttgatgattg acacgataaa taagtgggct tcattgtcct
     1981 ttgctaagtc aagggcttca gcgaacgctt tgatcgattg tcttgatccg tcaactgcta
     2041 ccatgatgtt ctggtatctt ttactcattg gaaccgcctc ctttttcttt tattgtaaca
     2101 tctcaagcaa aaatacaaaa aatatacgca atatgtaaat aaaatgatga acaaaagcaa
     2161 tttctttcaa gttatttata catttctttt ctcatcagaa cacctgttct aatcccatta
     2221 tttctgcatt atgtgaaatt cctcatcatt tttcacatcg agcgaacatc attctgttac
     2281 aaaaaacgtg gatttgtcat aaattgtcat gcttctttta cctatgaaat attttcacgt
     2341 gttaaagtat tcctcgttga acacaacaaa taaaactttt taacgaataa aggagatttt
     2401 tcatgacatc acttaaaact ttactttttg gaacaacttt ggctgccggc gctgcattct
     2461 tcatgggaac gactgctcat gcggacgaag cttataccgt tcaatcaggt gatactctat
     2521 caacgatttc tcaaaaatac gttggtgata actccttgat caatgccatt gcagaatcaa
     2581 attcaatttc tgatattaac ctgatctact caggacaaca attaacaatc ccaacagaag
     2641 gctctgcaca agcagctgct gaacctcaag cagccgttca agaagcacca gtccaagctg
     2701 aacctgttca agctgaacaa cctgttgtac aagaaacggt tcaaacggaa acacaagcag
     2761 cgcctgttgc tgaaacacaa cctgctcctg ctgtaacaga aactgctgct actccagcaa
     2821 gtacaagctc tgcaaaagaa tggatcgcac aaaaagaatc aagcggttct tacacagcaa
     2881 cgaacggacg ttacatcggc cgttaccaat tagattcttc ttacttaaat ggtgactact
     2941 ctgctgcaaa ccaagaaaga gtagcagaac aatatgttac ttcacgttat ggttcttggg
     3001 aagcggcgaa agcattctgg gaagcaaacg gttggtacta agattcatta tttgaacgtt
     3061 taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat gacctctttt
     3121 tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct tgtatcagaa
     3181 tctataaatc tactatttga atcaatctat taggttttaa actgtcccac cttcacacaa
     3241 attttttttg atttatttga atctggttcc gttgtttaaa atttttccta tattctttta
     3301 gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat aaaaaaacaa
     3361 attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg cctgaagcta
     3421 gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat tacatgtgtt
     3481 ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct tatgccatct
     3541 acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa aaaatctgtt
     3601 ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta actattttat
     3661 ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt tcatttggat
     3721 attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg aagtaattca
     3781 cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat aattttttta
     3841 ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca ataaataaaa
     3901 ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat aaggttactt
     3961 aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa gaaagcttat
     4021 cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc ttatggctat
     4081 acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt aggaatcgat
     4141 cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga acatgagtat
     4201 gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg cgctggccat
     4261 attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca aggattagca
     4321 ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc tgtaaataaa
     4381 aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta tcgtgaagat
     4441 gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc aaaaggaaag
     4501 ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata atatagcaga
     4561 caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga taaaaggctt
     4621 ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc gtatccccaa
     4681 cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc agactagttt
     4741 gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag attctgttaa
     4801 aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg tttctccatt
     4861 ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga ttcctaattc
     4921 aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt ctatttcttg
     4981 tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac gatttttatt
     5041 tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat tctcgtttaa
     5101 atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa taaacctgat
     5161 tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg ttcgatacat
     5221 tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta aaatattgat
     5281 aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt cgtagcgggt
     5341 gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag acggcagtaa
     5401 tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg gcttgacggt
     5461 aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta cttgagtcat
     5521 tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga agcctgcgat
     5581 gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc cagttgtttg
     5641 tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt ttaaagataa
     5701 tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa tattatcagt
     5761 gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat tcacaaaact
     5821 aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag ggatattcaa
     5881 aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata aaagcgagga
     5941 aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa tcaaaaaata
     6001 atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt tctttaatct
     6061 ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc tactttggta
     6121 ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac tcaaatgaga
     6181 agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag atgtatggta
     6241 aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt cgacattgta
     6301 tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa agaattgtcc
     6361 aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag caatctagtt
     6421 gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt tggtaagctg
     6481 atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca cgctctaggt
     6541 ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa cgcgaagaaa
     6601 atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca agaagatcag
     6661 attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt attacgtaaa
     6721 cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa cgaggctgac
     6781 gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa acgctgtatt
     6841 ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg ttgtgggaat
     6901 aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat tccttgatcc
     6961 gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc aggaagctga
     7021 taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat cagttcctgc
     7081 tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa tcggtcccct
     7141 ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat caatgtttca
     7201 gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt catgtccgtt
     7261 tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac cgcaagatct
     7321 aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc agcaaaagca
     7381 tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt cgtctctagg
     7441 taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag tttgtaagcc
     7501 agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa actagcagaa
     7561 aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct tggatcaata
     7621 caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc gtagtgatca
     7681 tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg aagcaattgt
     7741 tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc gcttaattga
     7801 taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc ccaatgatct
     7861 tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa acggcgatga
     7921 agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc ataagatagt
     7981 tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc aatcaaaaac
     8041 agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt tggatcaaat
     8101 aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa tgatagttcg
     8161 aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt gatctgattc
     8221 tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc attggcatat
     8281 gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc tttcgccgtc
     8341 aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc atcttcagct
     8401 acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc ttcttcccct
     8461 acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt aggtgcttct
     8521 acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag ttcacgctga
     8581 cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt gcgtacagtg
     8641 cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt tttaaaaaaa
     8701 gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc ttctgccgtc
     8761 tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact atagatatag
     8821 gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa atcttcaaat
     8881 cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt gaaaataccg
     8941 tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt ttttaaaaag
     9001 acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat tctcgctcct
     9061 tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg caaaataaat
     9121 tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac ctctttctct
     9181 ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa acataaaaaa
     9241 acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg atgaatctaa
     9301 tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt agaatatcca
     9361 aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta cctgtttttg
     9421 tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta tcaaggagga
     9481 atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg ttatttggtc
     9541 tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg aatcaaacaa
     9601 gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac tcactacgat
     9661 catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta tgtcagccca
     9721 ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg tagacatgat
     9781 gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa aacttatact
     9841 ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg aagcgtcagc
     9901 tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag tatcggtaga
     9961 accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac ccagctgttt
    10021 gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac gattgaacag
    10081 gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga ataaaatgac
    10141 agaaactaca ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa ttgaacgagt
    10201 acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc attatttagg
    10261 tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg gtcgtgcaat
    10321 cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc cttattttat
    10381 tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg acatggaaat
    10441 gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt ttaccacaga
    10501 agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg ggtgggcgga
    10561 aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg cccatcactg
    10621 cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga tcgccttttt
    10681 gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg gcagcgtaac
    10741 aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata cgtcttatat
    10801 cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt ttaattttta
    10861 ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt tctcttcgtg
    10921 tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt gggtttctca
    10981 aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt tatgtttttt
    11041 gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga atgggtgccc
    11101 atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga tgccagttca
    11161 caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc tgtcgtcgtt
    11221 ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga aaaaaaagat
    11281 acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat cattggtttg
    11341 cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc cattgttttg
    11401 atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa aacacctcgt
    11461 tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt attccaagtc
    11521 ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc tatcttgatt
    11581 ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc tgtcatgttt
    11641 ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt caaaatggaa
    11701 gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt cttagctatc
    11761 aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg gtatcgtatt
    11821 atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa accagaacac
    11881 ttctcttatt tgagaaatgt tctggttttt tattttcttt aataataatc attataaata
    11941 ataaaaacaa catcaagctt aatcaccaaa tgataaccat tatcgttctc atttagctgt
    12001 tattattatc aattacattt ttattatgct atccttgttt tgacaaggag gtctaataac
    12061 gatgactaat cagaaaaaag cttttgcagc aacaatcggc tgccttttat ttatgatctt
    12121 cggttttatc ttcgaccgat tctctcttac catcacccca cttttttata tcagtgccat
    12181 tatcatcggt ggatataaac aaacatcgga aggcttgaaa gaattgtgga atgatcggac
    12241 actgaatgtg gatttattga tggctttagc tgctattggt gcctgtttga tcggtaatta
    12301 ctttgaagga gcaatgttga cattcatctt ttgtttaagc ggtgcattag aggaatacac
    12361 aactaataaa agtcaaaaag aaattactgc tttgatgaat cttcaaccac aaaaagccca
    12421 attattaaaa gaaaatggac aaatgcaaga agtagatgtt tctcaactaa aggtggataa
    12481 cttggtattt gtcgctaaag gagcagctgt tccgatagat ggctttttag aaagcacaca
    12541 ggcaacgatc gatgagtctg cattaagcgg tgaatctgtg cctgtagaaa aatatttagg
    12601 cgatgagatg tatgcaggaa cgctgaatca aggaaatccc atcacgattc gagtaacaaa
    12661 aacgagttcc gaaactgtct ttgctaagat tatccaatta gtagaagaag cgcagaatac
    12721 gccaactcaa acagcttcat ttatcgaacg aatcgaaaat aattatgtaa aattgatttt
    12781 gcttgctgtt cctctaatga tccttctccc ccactttttc ctaggttgga gttgggatga
    12841 aagcttttat cgaggaatgg tattattagt tgtggcttct ccttgtgcgc ttgtcgcatc
    12901 tgctacacca gcttctctag ctgctctttc aaattctgca aaaaatggga ttttaatcaa
    12961 aggcggtatc catttagaaa agctttctga attaaaagcg attgcctttg ataaaacagg
    13021 aacgctcact aaaggaaaac cagttgtaac tgacagcttt ttctttgatg aaaaggattt
    13081 ggcacaacaa cttcttgttg ctatggaaca aaaaacgacg catccacttg ctcatgcgat
    13141 tttgcaatat ttcgattgca caatacctga agaaatccaa tacttaccaa tagaagaaat
    13201 tactggtttt ggattacaaa ctacctattt aggtgcgcaa tggaaagttg gcaagcacgc
    13261 atatgatcct gagacgatga taatcagcaa agagattgcg gaaatgatcg aacgtttgga
    13321 aaatcaaggc aaaaccgtga tttatctttc taaagatcag cagttaattg ctgttttagg
    13381 tctgctagat attccaaaag caaataccca acaagtaatt tcctacttta aatcccaaaa
    13441 catccataca agtatgataa ccggcgacca ttctggcaca gcaaaggcca ttgctgaaca
    13501 agtagggatt gaacattact atgcaagctg tactccggaa gaaaaaacac aactagtcaa
    13561 aaaagagaat gaactttacc aagtaaatgc gatggttggt gatggagtca atgatgcgcc
    13621 tgctttagca gccgcttctt taggtgtcgc tatggggcaa gggacagatg ccgctatgga
    13681 tatcgcagac atcgtcttaa tgaaaagcga cttaaataaa tttgtctaca gtcatcggct
    13741 atctttaaaa atgcggcgga tcatcaaaca aaatattatt ttttcgattc ttgttattct
    13801 cttgttgatt gcttctaact tcctgcaatt tttaaatcta cctcttggag ttgtaggaca
    13861 cgaaggaagc acaatattgg ttattctgaa tggcttgcgt ttactaagac ctttacctga
    13921 tctttctgat tcttcaaaaa aatgtaagtc ttgtccacta tacaaagcgc ggctgtcttc
    13981 tgtcaaataa gcaaagtgtt tttttatgat tttgtttatt ttatgaaaaa aacagcttat
    14041 gaataggtct tgaaggatga attcctactt ttgtaacttt ctaaaaacat gttatgattg
    14101 tttcgaagaa agtgaggaat atatttcatg gaaagttggc tttttttagg aattatttta
    14161 cttgtagcaa ttttagctaa aaatcaaagt ctgatgatag cggcagcttt tgttttgatt
    14221 atgaagctca taccagcctc ccgttccttt ttcccttggc ttcaaggcaa aggaatcaat
    14281 attggggtga cgatcatcac agcggctatt ctaatcccta tcgcaacagg agaaatcggt
    14341 ctacgtgatc tattagaagc ctttaaatcc cctattggct gggtagctgt tttttgcggt
    14401 gggctagtgg ctgtcttatc ttccaagggt gtaggattga ttgcttctga tccacaaatc
    14461 actgttgcac ttgttttcgg cacgatttta ggtgttgtct tcttaagagg aatcgccgcc
    14521 ggaccggtca ttgcttctgg tatcacttac tgtatcttac aagtatttgc tttaggaaaa
    14581 tagaacaaat tttgtctatt ttaggagatt cttaacaccc ccaatttact gatccgtgat
    14641 atactgacaa cgaggtgata caatgttcga aaaattaaag tatttcatag gaacaatcgg
    14701 ctggtttctt ctagcggtac ttttgattct gggcttagct tatctattat ttcctgattt
    14761 gatacaaaat gtgtttgata ctgctttttg attgacctat taaaacgata taggggatgc
    14821 tcctgcgatg caggggcttt ttttaatcaa tgttttgtaa taatttaggc cgtgccaatg
    14881 agacttccta tagaattcga tgctcttttt tctttttggc tcttctgctc ttttttgtta
    14941 tactagatta cagtggacaa aagaaagaat gatcgcatcc acttcaacga atatcaaaat
    15001 ggaagaatta gtgcctgttt tcaggttact ttgaaaagaa aggaagacgc ttgtggaaac
    15061 ttttgaaggt cttttaattg atacgtacgt tttacaagaa cagccaatga tgctgactgg
    15121 gatcaacttg cagcttgagg accgtgtgat caatggcctg cttaaagatc gctatttggc
    15181 aaaatatatc ttacgtttgc ccctaaataa atatgtgctg gaagtaagtg gcaaatggaa
    15241 ccgtcggaac cagttggtca taaaaaaaat gttgataaaa aatatcgatg aa
//
LOCUS       DACDIP010000059        15244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDIP010000059 DACDIP010000000
VERSION     DACDIP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.059"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IV257_002121"
     CDS             168..362
                     /locus_tag="IV257_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815219.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IV257_002122"
     CDS             631..930
                     /locus_tag="IV257_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815220.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IV257_002123"
     CDS             complement(1027..1461)
                     /locus_tag="IV257_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8815221.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IV257_002124"
     CDS             complement(1693..2403)
                     /locus_tag="IV257_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8815222.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IV257_002125"
     CDS             complement(2393..3253)
                     /locus_tag="IV257_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8815223.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IV257_002126"
     CDS             complement(3258..3896)
                     /locus_tag="IV257_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8815224.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IV257_002127"
     CDS             complement(3911..4210)
                     /locus_tag="IV257_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8815225.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IV257_002128"
     CDS             complement(4253..5737)
                     /locus_tag="IV257_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8815226.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IV257_002129"
     CDS             complement(5757..6218)
                     /locus_tag="IV257_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8815227.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IV257_002130"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IV257_002130"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8815228.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IV257_002131"
     CDS             7521..8291
                     /locus_tag="IV257_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8815229.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IV257_002132"
     CDS             complement(8564..9391)
                     /locus_tag="IV257_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8815230.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IV257_002133"
     CDS             9959..11194
                     /locus_tag="IV257_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8815231.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IV257_002134"
     CDS             complement(12193..12453)
                     /locus_tag="IV257_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8815232.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IV257_002135"
     CDS             complement(12480..13781)
                     /locus_tag="IV257_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8815233.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IV257_002136"
     CDS             complement(14073..14531)
                     /locus_tag="IV257_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8815234.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..>15244)
                     /locus_tag="IV257_002137"
     CDS             complement(14544..>15244)
                     /locus_tag="IV257_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8815235.1"
                     /translation="KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITK
                     NFPSNIPNNYLEYYLYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVL
                     NFNFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEP
                     ITLRKPISDFHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILD
                     EMIKVNKKYWPALK"
BASE COUNT         4877 a         3104 c         2365 g         4898 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattaggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcaaatgcgt
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacgac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttat
//
LOCUS       DACDIP010000060        14487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDIP010000060 DACDIP010000000
VERSION     DACDIP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.060"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(449..2446)
                     /gene="tkt"
                     /locus_tag="IV257_002138"
     CDS             complement(449..2446)
                     /gene="tkt"
                     /locus_tag="IV257_002138"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8815236.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2548..2799)
                     /locus_tag="IV257_002139"
     CDS             complement(2548..2799)
                     /locus_tag="IV257_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8815237.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2946..3569
                     /gene="lexA"
                     /locus_tag="IV257_002140"
     CDS             2946..3569
                     /gene="lexA"
                     /locus_tag="IV257_002140"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8815238.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3748..3966
                     /locus_tag="IV257_002141"
     CDS             3748..3966
                     /locus_tag="IV257_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815239.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4254..4766)
                     /locus_tag="IV257_002142"
     CDS             complement(4254..4766)
                     /locus_tag="IV257_002142"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8815240.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4780..7077)
                     /gene="recJ"
                     /locus_tag="IV257_002143"
     CDS             complement(4780..7077)
                     /gene="recJ"
                     /locus_tag="IV257_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8815241.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7219..7653)
                     /locus_tag="IV257_002144"
     CDS             complement(7219..7653)
                     /locus_tag="IV257_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8815242.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7663..8451)
                     /locus_tag="IV257_002145"
     CDS             complement(7663..8451)
                     /locus_tag="IV257_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8815243.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8448..9389)
                     /locus_tag="IV257_002146"
     CDS             complement(8448..9389)
                     /locus_tag="IV257_002146"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8815244.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9566..10153)
                     /locus_tag="IV257_002147"
     CDS             complement(9566..10153)
                     /locus_tag="IV257_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8815245.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10374..11687)
                     /gene="obgE"
                     /locus_tag="IV257_002148"
     CDS             complement(10374..11687)
                     /gene="obgE"
                     /locus_tag="IV257_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8815246.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11975..12985
                     /gene="gap"
                     /locus_tag="IV257_002149"
     CDS             11975..12985
                     /gene="gap"
                     /locus_tag="IV257_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8815247.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(13070..14005)
                     /locus_tag="IV257_002150"
     CDS             complement(13070..14005)
                     /locus_tag="IV257_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8815248.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14191..>14487
                     /locus_tag="IV257_002151"
     CDS             14191..>14487
                     /locus_tag="IV257_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8815249.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYLHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         4052 a         3226 c         2552 g         4657 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaggagcaa tcgctttttt attgatcacc atttttcttt taggtctatg gtgtcgggca
      181 ctgcgggtct tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa
      241 gttctcttct taaaacaaac ttaattaatt aaattatact tttaattctc aagaaacaca
      301 aactaatccg aagaattctt tgttttgtca tttaaatgta acatagccga aaacgaaaag
      361 taatattgat ttattcttct aacacataaa gaacagctgt ctccaacttg cagacagctg
      421 ttcttatcag tcaaacaatt gcttggatct atgatagttg attgtacgta ttcacgacat
      481 tttctactgt aaatccaaat tcttctaaaa ttttagtgcc aggggcagat gcaccgaaat
      541 gatcgattgt aatcgttgcg ccctctgttc ctacgtagcg ttcccaacca aatgatgcag
      601 cagcttcgat agccacacgt ttcttcactg atttaggaag tacactttct ttatattcag
      661 aagattgttt ttcaaagagg tcaaagcttg gcatagaaac aacagaaaca tctttgcctt
      721 tttcagccaa ttctttttga gctttcacag ccaaatcaac ttcagaacct gttgcaatca
      781 aaataccttc tggtgtttct ccttgtgatg gagaaagaac atacgcgcct tttttcacca
      841 tttcatctgc tacttctttt gtagaaggca atactggaag gttttgacga ctcaatacaa
      901 ggatagtagg cgcatctgtt gtctccatcg ctactttcca agcagcacgt gtttcatttc
      961 cgtctgccgg acgaatcact tgaacaccag gcatgcagcg aacagatgct agttgttcaa
     1021 tcggttcgtg cgttggtccg tcttctccga cagcaaccga gtcatgtgtt aagacgtaaa
     1081 caacaggagt atgttggatt gctgctaaac gaactgctgg tcttagataa tcgacaaaga
     1141 caaagaatgt tccaccgtag atacgagttc cgccatgaag ttggatacca ttcatagccg
     1201 aagccattgc aaattcgcgg acaccgaacc agatgttgcg tccttcataa tgttcaggag
     1261 tgaaatcttt atctgcagcc accattgtat tattggaacc tgacaagtcc gctgaaccac
     1321 cccagaagct tggtatagct ttcgataatt cttggatgac ttctttactt gaaacacggc
     1381 ttgcttgact gcttccaact tcataagttg gcaattctgc atcccagttt tctggtagtt
     1441 tgccatcaaa agcatcttca aattgttgtg ctaattcagg ataagctttt ttataattgg
     1501 caaacatttc attccatgca tcttcagctt tttgtccttc ttctatcatc gtttgatgga
     1561 agcgggcagc tacttcttct ggaacagtga aatctggata ctcccaacca taaacttctt
     1621 tagccatctt gatgccatct tcaccaatag gagcaccatg aacagcagat gttccttctt
     1681 taggagcacc atagccgatg actgttttta cttcgatcaa tgttggtttg tctgtttcag
     1741 cttttgcttc ttcaatcgct ttagagattg cttctaggtc atttccgtct ttcactaaga
     1801 tatgttgcca gccatatgct tcatagcgag caccaacgtt ttctgtgaaa gcttttgaag
     1861 ttggaccgtc tagtgaaata tcgtttgaat cgtataaaac gatcaatttc cctagtttca
     1921 tatgacctgc catcgaactt gcttcttgag ataccccttc catcaagtct ccgtcaccgc
     1981 atattgcata cgtatagtga tccatcacat taaagttttc tttgttatac gttgctgcaa
     2041 ggtgcgcttc tgccattgcc ataccgacag ccattgcaat cccttgtcct aatggccctg
     2101 tagtggcttc tacaccatct gtgtgatgta cttcaggatg tcctggtgtt ttagaatccc
     2161 attgacggaa ttgtttcaaa tcatcgattg tcacttgata gccagctaaa tgaagcaagc
     2221 tatacaacat tgctgaccca tgtcctgccg aaagaacgaa acggtcgcgg tcagcccagt
     2281 tttttgatgt cgttggattt actttcaggt gttttgtcca taacgcatat gccataggtg
     2341 ctgcgcccat cggcaatcca ggatgtcctg agtttgcttt ttggactgct tcaatactta
     2401 atgtacgaat tgtgttaacg ccaagttggt caattttgtc aaacaaaata acccctccta
     2461 ttaatttagg ttttttttac ctttatttct tacactttca ttctataaga tgtcacacga
     2521 aaaagcaatc tttttcagta ttttttacta aaagttgtca tttcggttgt gtaagccttt
     2581 tttcttttgg atttctttta atttttccgg ggtcacgtca ttgccctcag gatcgacgac
     2641 tttcattcct tcgatatgat gacgcatacc ggaacgaaaa gcttccaaat actctttgcg
     2701 taagttgatt tgttcttgtt cttcggcttt tgagagacca ttttccttct ttttacgtgc
     2761 aagttcattg atcctctcta ttttttcagg tgacagcatg ctcagccctc cttttactct
     2821 ccttcatatt atataaaaac tgaaaaaaaa acaactagat aaactaaata aaatacgaac
     2881 attcgtttga tttagcgctt tttatatggt actatttatt tatcaagtga aagaaggtgt
     2941 accttgtggc ccgacaaact gaatcaagac aaatcgaagt cttacgatat atccatgaac
     3001 aagtaaccca aaaaggatac ccgccgactg tccgtgaaat cggggaagct gttcagcttt
     3061 cttctacttc tactgttcac ggtcatcttg cacggcttga aaaaaaaggg tttatccaaa
     3121 gagatccgac gaaacctcgt gcaatcgagt tgacaaaaca aggtttagaa aagattggga
     3181 ttcgtccaac aactattcca gtacttggcg tcgtaacagc tggagaaccc attttagcag
     3241 tagaggatgc ttcagacttc tttccaatcc ctcctaatct ttcttccgaa gaaggcagtc
     3301 tctttatgct gacaattcgt ggagatagta tgatcaatgc tgggatcttg gatggtgaca
     3361 acgtgatcgt tcggaagcaa gaaactgctc aaaatggcga tattgtcatt gcaatgactg
     3421 ccgaggacga agcgacttgc aaacgattct ataaagaaaa aaactttttc cgtctgcagc
     3481 ctgaaaacga cttgctcgaa ccaatcatct tagatcaagt atctatatta ggacgagtag
     3541 taggacttta tcgtagtcat atctattaat tgctactaat aaaagaaaac tttttaaaaa
     3601 gagtttgaga cgatggtctc aaactctttt ttcgcccggg ttcttcttct ttcaaatttt
     3661 tatcacttcc ctctttacaa accgaacaaa tattcgtata ctaaatatac gaacaattgt
     3721 tcgcataaat taacgaggtg agaattcatg aaatcaatga gacgtcttgg cttagtatta
     3781 actgttttgt ttttaggtat tattattggt aatttcggca tacgtccagc tttacttgtg
     3841 tttgttccgt tatttacttt gtggttcatg ttgtgggatg aaaagaagta ccgtcaatct
     3901 gagcggcgca gaattcatga agatcatttc tatcatgagc cctactacag atccaggcaa
     3961 tcataataag gattgactgc taattattca aaagacaaga agttaaactt ttacgggggt
     4021 gtgacaaaaa tcggtcacat ccttttttcg aataaacggt gttctaaagc cagtttttcg
     4081 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
     4141 ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc
     4201 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
     4261 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
     4321 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
     4381 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
     4441 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
     4501 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
     4561 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
     4621 attcgttttt cttttgcata atttacgatt tgcttcgtag cttcccgata agcgtcacca
     4681 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
     4741 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
     4801 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgccg
     4861 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
     4921 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
     4981 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
     5041 ttcttgagaa tggatcagtt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
     5101 caaataggcc tcatcaggcg aaatccccat cagataaaca cgattgaatg aataagaccg
     5161 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataataacta gagagttcgg
     5221 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     5281 ctggataatc tcaggcacga atttcaacgt agaagaatca aatgctaaat aaaggctacc
     5341 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     5401 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     5461 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     5521 accaaaagcg accgcatcaa gctgactcga tgcattggaa aggacaaatt tcagatgctg
     5581 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     5641 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     5701 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     5761 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     5821 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     5881 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     5941 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     6001 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     6061 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     6121 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     6181 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     6241 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     6301 cgcctgaagt cctatccgtt ctgaatgatg aatggcattg attcctaatg aaacaaggat
     6361 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     6421 aaattctaca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     6481 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     6541 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     6601 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     6661 gattccttct tcgatctttt cttggtatac agattggttt ggtccatacc catgctcaaa
     6721 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     6781 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     6841 gattacagct tcttggattc ggctgattgc tttatccata tcgtgcatca gatacggatc
     6901 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     6961 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     7021 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     7081 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     7141 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     7201 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     7261 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     7321 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     7381 ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     7441 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     7501 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     7561 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     7621 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     7681 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     7741 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     7801 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     7861 atcagcctta tcgaagaaat ctgtctcgat tggacctgga ttcactgtcg taacagctac
     7921 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     7981 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagacg cgatattaat
     8041 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     8101 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     8161 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     8221 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     8281 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     8341 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     8401 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     8461 tcctcgcgaa atggaatctc aatgctatca aaatctttca tgatcgtcgt gttttcaaac
     8521 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     8581 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     8641 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     8701 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     8761 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     8821 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     8881 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     8941 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     9001 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     9061 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     9121 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     9181 aaaatatgat cgccgtgtaa atgtgtaata aaaattttac cgatttttct cggacgaata
     9241 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     9301 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     9361 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     9421 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     9481 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     9541 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     9601 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     9661 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcgg gtaatttctc
     9721 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     9781 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     9841 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     9901 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     9961 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
    10021 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
    10081 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
    10141 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
    10201 tgtaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
    10261 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
    10321 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
    10381 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
    10441 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
    10501 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
    10561 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
    10621 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
    10681 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
    10741 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
    10801 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
    10861 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
    10921 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
    10981 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
    11041 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggg
    11101 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
    11161 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
    11221 tctagttcga tttttcgctc ttgtcctggt tcgccgtttt cagcaatttc cggtgcagga
    11281 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
    11341 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
    11401 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
    11461 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
    11521 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
    11581 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
    11641 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
    11701 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
    11761 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
    11821 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
    11881 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
    11941 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
    12001 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
    12061 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
    12121 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
    12181 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
    12241 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
    12301 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
    12361 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
    12421 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
    12481 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
    12541 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
    12601 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
    12661 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
    12721 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
    12781 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
    12841 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
    12901 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
    12961 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
    13021 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
    13081 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
    13141 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
    13201 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
    13261 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
    13321 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
    13381 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
    13441 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
    13501 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
    13561 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
    13621 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
    13681 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
    13741 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
    13801 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
    13861 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
    13921 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
    13981 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
    14041 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aacccgcgaa
    14101 ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat tgtatttccg
    14161 acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac catagacgaa
    14221 ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat cgctaaccga
    14281 atgggacgtg ctatacaaac tatttataat gtagtcaata agttcaagca aggcaagact
    14341 gctcttgatt atttgcacca gtataaagaa aataagaaaa aatgtggtag aaaagtcatt
    14401 caattacctg ctcatgaagt agattacatt aaagagaaag tcactcttgg ttggacgcct
    14461 gacgtcatta tcgggcgaaa agaaagg
//
LOCUS       DACDIP010000061        14339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDIP010000061 DACDIP010000000
VERSION     DACDIP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.061"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..587)
                     /locus_tag="IV257_002152"
     CDS             complement(<1..587)
                     /locus_tag="IV257_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815250.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
     gene            complement(735..1277)
                     /locus_tag="IV257_002153"
     CDS             complement(735..1277)
                     /locus_tag="IV257_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8815251.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1412..1801
                     /locus_tag="IV257_002154"
     CDS             1412..1801
                     /locus_tag="IV257_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815252.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1805..2569
                     /locus_tag="IV257_002155"
     CDS             1805..2569
                     /locus_tag="IV257_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8815253.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2698..3645
                     /locus_tag="IV257_002156"
     CDS             2698..3645
                     /locus_tag="IV257_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8815254.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3775..4515
                     /locus_tag="IV257_002157"
     CDS             3775..4515
                     /locus_tag="IV257_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815255.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4806..5474)
                     /locus_tag="IV257_002158"
     CDS             complement(4806..5474)
                     /locus_tag="IV257_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8815256.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            5608..5895
                     /locus_tag="IV257_002159"
     CDS             5608..5895
                     /locus_tag="IV257_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815257.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            6311..7849
                     /locus_tag="IV257_002160"
     CDS             6311..7849
                     /locus_tag="IV257_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8815258.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7846..8811
                     /locus_tag="IV257_002161"
     CDS             7846..8811
                     /locus_tag="IV257_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815259.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8798..10120
                     /locus_tag="IV257_002162"
     CDS             8798..10120
                     /locus_tag="IV257_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8815260.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      10183..10287
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10361..11104
                     /locus_tag="IV257_002163"
     CDS             10361..11104
                     /locus_tag="IV257_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8815261.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            11119..12111
                     /locus_tag="IV257_002164"
     CDS             11119..12111
                     /locus_tag="IV257_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8815262.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            12123..12860
                     /locus_tag="IV257_002165"
     CDS             12123..12860
                     /locus_tag="IV257_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815263.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12850..13572
                     /locus_tag="IV257_002166"
     CDS             12850..13572
                     /locus_tag="IV257_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8815264.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13656..14279)
                     /locus_tag="IV257_002167"
     CDS             complement(13656..14279)
                     /locus_tag="IV257_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8815265.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4798 a         2429 c         2965 g         4147 t
ORIGIN      
        1 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
       61 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
      121 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
      181 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
      241 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
      301 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
      361 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
      421 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
      481 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
      541 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
      601 taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg atgtttttta
      661 atcaaaataa taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt
      721 tttaagtttg tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct
      781 gaaatatatt ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg
      841 cgatacagtt cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct
      901 tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc
      961 aaacttttat cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat
     1021 gccaatttgt cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag
     1081 acaaataaat tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa
     1141 atcgtgatct cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac
     1201 atgaaacgta acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac
     1261 atttttggat caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa
     1321 atcgtaagtg aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa
     1381 tagaattata aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc
     1441 cagccacgtg ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc
     1501 aattgaggta gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt
     1561 ttttttcttt ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa
     1621 tttttctatt tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg
     1681 cttatttccc gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca
     1741 gacaactgtt ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta
     1801 acaattgaat ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt
     1861 tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac
     1921 aagtaaggag ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat
     1981 cctgccagat gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta
     2041 tacattttgg gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt
     2101 agaaaaaaga ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat
     2161 gacaacatgc gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg
     2221 ttcccctgca cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta
     2281 tgtcgtacca aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt
     2341 gcagccggaa aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa
     2401 aatcccttat atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa
     2461 agaaattgtt tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga
     2521 agaagcttcc acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa
     2581 aatcactcat gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt
     2641 aggaaaattt ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg
     2701 cccactttag cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc
     2761 atcaatcatc cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct
     2821 gaactggatt accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc
     2881 atcaaattat ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg
     2941 atgggaatcg ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat
     3001 gccttcgacg tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc
     3061 gattggatcg accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat
     3121 gattatgtag acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa
     3181 agagattatc aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca
     3241 agggaaactg gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg
     3301 tttccaaatc attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca
     3361 gataaaactt gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt
     3421 ataaatgctg gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt
     3481 ttagaccaaa tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt
     3541 gaagtatgtg gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat
     3601 cgtcgttttt ttcctgaact agttatcaga gagacaacga attaagtctt attactatat
     3661 atatatttat gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac
     3721 aatgaatctg tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa
     3781 gaaatcagac gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta
     3841 accaacgaca agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat
     3901 cagtccccgt ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc
     3961 cgtcgcaagc gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa
     4021 aagcataaag gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca
     4081 ggacgaaaaa aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca
     4141 gagtccaagc atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat
     4201 tttgttccta aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct
     4261 gaggatcatt taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca
     4321 gaaccgacgc cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga
     4381 acaaaaacgt tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt
     4441 atcttagaac gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat
     4501 agctatttta aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct
     4561 gcggcgtttc cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt
     4621 gagtttaagc gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa
     4681 actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga
     4741 ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat
     4801 aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt
     4861 tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt attcaatgta
     4921 taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc caaataaagc
     4981 aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga
     5041 aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga
     5101 aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga
     5161 ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca
     5221 aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc
     5281 aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc
     5341 aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat attgattcct
     5401 gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa
     5461 tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat
     5521 actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt
     5581 tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca
     5641 aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg
     5701 cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc
     5761 aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct
     5821 gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat
     5881 aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt
     5941 ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat
     6001 aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa
     6061 agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat
     6121 ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct
     6181 ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa
     6241 ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa
     6301 ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga
     6361 agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc
     6421 tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat
     6481 cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt
     6541 agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt
     6601 ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc
     6661 atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt
     6721 cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc
     6781 attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc
     6841 tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact
     6901 gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt
     6961 gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac
     7021 gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat
     7081 tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt
     7141 gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct
     7201 agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac
     7261 atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat
     7321 ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact
     7381 taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga
     7441 atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc
     7501 aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa
     7561 tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga
     7621 agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc
     7681 taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacagagga
     7741 tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc
     7801 acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc
     7861 gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg
     7921 gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact
     7981 actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga
     8041 aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag
     8101 caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg
     8161 ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc
     8221 ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa
     8281 cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca
     8341 tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac
     8401 gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta
     8461 ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag
     8521 ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg
     8581 aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa
     8641 aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg
     8701 gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa
     8761 gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat
     8821 catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac
     8881 agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc
     8941 taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa
     9001 aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt
     9061 tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct
     9121 ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca
     9181 tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa
     9241 ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat
     9301 ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag
     9361 caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt
     9421 agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac
     9481 ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt
     9541 agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga
     9601 gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag
     9661 caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc
     9721 agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc
     9781 tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg
     9841 agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg
     9901 gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca
     9961 aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt
    10021 gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta
    10081 tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg
    10141 cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg
    10201 tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact
    10261 ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag
    10321 cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc
    10381 agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat
    10441 acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca
    10501 agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc
    10561 tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc
    10621 tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact
    10681 taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta
    10741 ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat
    10801 ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc
    10861 gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc
    10921 agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa
    10981 aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt
    11041 attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt
    11101 ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta
    11161 tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa
    11221 gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa
    11281 gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat
    11341 tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa
    11401 gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg
    11461 atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa
    11521 gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa
    11581 gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga
    11641 gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat
    11701 atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat
    11761 gaacggttag attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat
    11821 ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag
    11881 gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt
    11941 ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga
    12001 ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc
    12061 aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag
    12121 atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag
    12181 acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg
    12241 gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca
    12301 aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag
    12361 atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc
    12421 atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct
    12481 atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg gccttgttac
    12541 gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg
    12601 ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga
    12661 ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg
    12721 tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt
    12781 cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag
    12841 gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc
    12901 cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag
    12961 gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg
    13021 aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc
    13081 ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta
    13141 tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa
    13201 ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat
    13261 tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt
    13321 ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac
    13381 cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt
    13441 tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa
    13501 gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga
    13561 ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt
    13621 gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt tgcttcccag
    13681 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    13741 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacgtccg
    13801 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    13861 tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    13921 gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt
    13981 tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag
    14041 ccttctgttg ggattgttaa ttgttgtcct gagtaaatca ggttaatatc agaaattgaa
    14101 tttgattctg caatggcatt gatcaaggag ttatcaccaa cgtatttttg agaaatcgtt
    14161 gatagagtat cacctgattg aacggtataa gcttcgtccg catgagcagt cgttcccatg
    14221 aagaatgcag cgccggcagc caaagttgtt ccaaaaagta aagttttaag tgatgtcatg
    14281 aaaaatctcc tttattcgtt aaaaagtttt atttgttgtg ttcaacgagg aatacttta
//
LOCUS       DACDIP010000062        13723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDIP010000062 DACDIP010000000
VERSION     DACDIP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.062"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(506..1225)
                     /locus_tag="IV257_002168"
     CDS             complement(506..1225)
                     /locus_tag="IV257_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8815266.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1274..2922)
                     /locus_tag="IV257_002169"
                     /pseudo
     CDS             complement(1274..2922)
                     /locus_tag="IV257_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            3101..3691
                     /locus_tag="IV257_002170"
     CDS             3101..3691
                     /locus_tag="IV257_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8815267.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3783..5288
                     /locus_tag="IV257_002171"
     CDS             3783..5288
                     /locus_tag="IV257_002171"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8815268.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5372..6724)
                     /gene="celB"
                     /locus_tag="IV257_002172"
     CDS             complement(5372..6724)
                     /gene="celB"
                     /locus_tag="IV257_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8815269.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6724..7242)
                     /locus_tag="IV257_002173"
     CDS             complement(6724..7242)
                     /locus_tag="IV257_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815270.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7258..7584)
                     /locus_tag="IV257_002174"
     CDS             complement(7258..7584)
                     /locus_tag="IV257_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8815271.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7597..9576)
                     /locus_tag="IV257_002175"
     CDS             complement(7597..9576)
                     /locus_tag="IV257_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8815272.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9597..9911)
                     /locus_tag="IV257_002176"
     CDS             complement(9597..9911)
                     /locus_tag="IV257_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8815273.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10079..10372
                     /locus_tag="IV257_002177"
     CDS             10079..10372
                     /locus_tag="IV257_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8815274.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10450..11562)
                     /locus_tag="IV257_002178"
     CDS             complement(10450..11562)
                     /locus_tag="IV257_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8815275.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11949..12128
                     /locus_tag="IV257_002179"
     CDS             11949..12128
                     /locus_tag="IV257_002179"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815276.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12322..12492
                     /locus_tag="IV257_002180"
     CDS             12322..12492
                     /locus_tag="IV257_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8815277.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12916..13656)
                     /locus_tag="IV257_002181"
     CDS             complement(12916..13656)
                     /locus_tag="IV257_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8815278.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4260 a         2797 c         2364 g         4302 t
ORIGIN      
        1 taggagaggt tgtgaaatca gcgttttggc ctgagtatta gagaaaaaaa cgggaaaata
       61 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
      121 tatcagaggt tgtgaaatca gcgttttggc ctgagtatta gagaaaaaaa cgggaaaata
      181 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
      241 tatcagaggt tgtgaattca gcgttttggc ctgagtatta gggagatcgt gtccttacta
      301 tctaactctg agtcacaagg aacaatgggg actgtaacaa aagtcttgtc acagtccctt
      361 attccgaaca aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa
      421 aaagccatcc gaaagtcaga cggacaagtc tgcttttcag atggcgctac aactactctg
      481 cttattcttt tagctatcca ttcaattatt tttctctcac ttgttctagc tcttctttgg
      541 tcatgggtcg atattgccct ttattcaaat ctttaggcag ccaaaatttt cccatacgga
      601 ttctttttaa atataagact tcttttccta ctgctttgac cattcgctta acttggtgaa
      661 actttccttc atgcaaaatc agacggataa ttgaagtttc tgtctcttga tctacttgat
      721 caatttggag ttttgccggc aatgtctgct catcgccatc aataatcagt ccttctgcaa
      781 ataaacggat atcttcttct gtcattatac cgctaacctc agctagatat tctttctcca
      841 catgtttttt aggtgatagt aactgatggg caaacacccc gtcattcgtt aaaatcaaga
      901 ggccctctgt gtctttatct aatcggccga ctggaaaaag atcttcccga taatcctggt
      961 cttccaataa atcgattacg gtttgatcat gattatcctg agtagcagaa atgactcctg
     1021 caggtttatg caaaatgtag taaaaatctt tttgatacgt aatgatttct cctaaataag
     1081 taaccgcatc ttcgaattca tttacttgcg ctttatcgct tttcaccact tgtccattga
     1141 cttcgatcgt tccttttttg attaatcctt tgacttcttt gcggctgcca agtcccatat
     1201 ctgctaaaaa cttatctaaa cgcatgcgtt tcccccattt caaaagagca tcaatctagc
     1261 tcttctgatt cacttatttt atttttaatt tatgtcgtaa acgcgctatt cctggtccta
     1321 gtagtctttc tgccaagcgg attttcaaag ctaaataacc atacactaaa gcccctatac
     1381 ctgctactac aagaatcaac aatagagact ggaatttgct atcttcgttt aaaaaagcac
     1441 caaaaatcat ctttgtgatc cacgaagcca aaagcataat caaggtaaga ataaagatca
     1501 gcaatgtgtc ctgcgccaga caaaacttgg tttaaagcgg gctacttcat ggattctctt
     1561 taagatcaag gtacaagaca cagcaaatcc gatcattgtc gccaataaag ggccgtatac
     1621 ttcaaataaa cgaatggctg ggtattgaag aaggagtttc actagaaacc caataaccaa
     1681 ataattaatt gccgccttgt tttcaaacat tccttgcagc atgttggaaa ctagcatata
     1741 tagtcctagg aagagtccta caaaacttgc ttggatcagt acattacttc ctagagaatc
     1801 cggtgtataa aataatgtat ttagcggata ggcaagtaac ataacaccaa atgtcgccgg
     1861 aaacataaaa aatgaaaaca actgtaaatt tccgcttgtc aattttgcca gacctactcg
     1921 atctttcaac gtaacagctt ctgtaatcaa cggtaaccca gtagacgcga tcgatgttgc
     1981 caatgcaata actaccatcg tcaacttatc tggattggca ctaaaaatcg aaaaaagatc
     2041 cagtaactgt gcattggaat actcggtgga tgcagacatg atcttcataa aagtgaactg
     2101 atcgactaat ttgaagatcg ttacccctga acccacaata ataaacggaa tcgcttcttt
     2161 aacggtatct aacaacaatt ccttcgttgc aatatttatt ttgttttcgc tgtagtgaat
     2221 aaacgccgat gtatacgctt gatgtttctt caaaaaatac aaaaggacaa gaatacttgc
     2281 tagcataccg ataaatgccg cgaaagttga ttgcgtgacc gccgtcacat agtcaccatc
     2341 catgactttc atgatgatga atgtagccaa tagcatataa aaaatacgtg ccacctgttc
     2401 tacaatctgc gacagcgcat aaggcatcat ttcttgattg ccttgaaaat accctcgtat
     2461 cacactcata caaggaaaaa ccaatacagc gatactcaaa gaacgcatcg ttggaatcag
     2521 ttcttctccg ccaccagaag catgagctag ccaaggagag gcgatgtaca tgaacaaagc
     2581 aaacaacagg cctaacccgc ccatcatctg taatgcacgg ataaataatc gtcgtgaagt
     2641 gccatattca ttcaatgaat tatagcgtgc agtttgtttt gcaatcgcag caggtattcc
     2701 tgctgtagag atcagtagaa acaatgcata tatattgtat cccatattga ataatccatt
     2761 ggcagcctta gcattttcgc ccatccaagc ataccaggga atgatataga cagcgcctag
     2821 caatcttgaa atgatattac tggcggtcat ccaagcagag cctcgtgcca ttttttcttg
     2881 cggtgttaat tgatctaatg ctggttttct ttgattatcc atcggtcaga ttccaacttt
     2941 ctacaaaaca ttactatcat tttaattggt aaaacagaaa ctggcaatcg attttatata
     3001 acttttataa gaacaattgt ttttttgaat tcggtttcgt ttttttggta aagtaaagaa
     3061 atatacagcc ttttatggct aacatttata ggagatatca atgacacaag aaataaaaaa
     3121 actacccgtt atatttttag gtcttttctt tatcacagtt ggattgaatt ggttcttgct
     3181 ccctcacgat atagcttccg ccggagtggg cgccatcgga catttagttg aaacggcctt
     3241 ttctatccaa cgctcagtta ctgtttggag catcaatctt atgatgctgc ttctagccgc
     3301 tgtcctatta ggaaaatcag tattttttaa gactgtagcg ggaagtctgc tttttcctgt
     3361 tgttttagag attgttccaa aagtggaaat gatctcctca catttctttt cactgatttc
     3421 aggaagtctt ttgttcagtc ttggggttta cacattgtac acagtcgggg cctcaaatgg
     3481 cggtataacg ataccgccga ttattttcca caaatttttt cggcttccaa tgccgcaagg
     3541 acttttactt accaatagtc ttatcgtctt tttgaattat tatgtttttg gattgttgga
     3601 gacactattc gttctcctat ctattacagt cagttcattg tttatgaaaa tactcatccg
     3661 cttgtcccct gtgtcaagaa aatttgaata aagctggatt ttgtcatagc ggaaacaaac
     3721 gatatattaa aaagagtgtg ctataataaa ccaagaaatt gaataaagcg agggatcaat
     3781 caatgagctt ctctttagaa gacatccgtc atttactatt aaaagaaaat ctattaaaag
     3841 aatttgtttc aacaagaggc tggcatcttg atgtaccaga aaaagctgaa tttcttcagt
     3901 taagttatga ttcaagaaaa gctgatgctt ctactttgtt tttctgtaaa ggaatgaatt
     3961 tcaaagaaga atatttggat tcagcaatcg aacagggtat tcaatattat gtatcagaac
     4021 aaccatacga aaattcagca gtaggaatca tcgtaacgga tatccgtaaa gcaatggcgc
     4081 ttcttgctat ggctttttac gactatccgc aaaataaatt gaaagtgatc ggattcactg
     4141 gaacaaaagg taaaacaact gctgcgtact ttacaaaagc aatcttggat cacacaacaa
     4201 ataaaaaaac agcattgctt tctactatga atacaacgtt agatggcaaa acatatttca
     4261 aatcccattt gacaacgcct gagtcattag atttatatcg tatgatgtca gaagcagttg
     4321 aaaatggtat gactcatcta gtgatggaag tttcttcaca agcttacaaa acacaacgcg
     4381 tatacggctt gactttagat gtcggaatct tcttgaatat ctcgcctgac catatcagtc
     4441 cgatcgaaca tccgacattt gatgactatt tctattgcaa gcgacaattg atcttgaatt
     4501 caaaaacagt tgttttaaat catgaaagtg actattttga tttacttagg gaaacagccg
     4561 atctattcca gattccaacg atcacttatg gtcgttcgga agaaagcaat tatcaagtga
     4621 tccgttcaga taaaggtact cacggtttta ctttatcttc tcatgaagat cgcttagcta
     4681 tctgcgatac tgcttatgat attttgctgg ctggaggatt caaccaagaa aatgcagcca
     4741 gtgcgatcat tgccgcagca ctaacaggtg cctccagtca agatgcacaa gacggattaa
     4801 aagaagctcg tgtaccaggc cgaatggatc aattgatcca atccaatgga gcgcatgtct
     4861 atgtggacta tgcacataat tatctaagct tgaaaacatt gctgaaattt gccaaaaatg
     4921 aacatccaga tggccgtgta attgttgtat taggcagccc aggaaacaaa gcaatctccc
     4981 gtcgacatga ttttggaaaa gtgctatcag aaacagctga catcgccttt ttgacggcag
     5041 atgacccagc atttgaagat ccgaaaaaaa tcgcggaaga aatcaatgaa gcaattacta
     5101 atccagattt gatcgttcat tatgaaatgg atcgtcctga agcgattcga cgcgctttag
     5161 cagaaagcgc tccgcaagat tcagtcgtta ttgcgggaaa aggcgtggat ccctatcaaa
     5221 aaatcaatgg tgtagatgaa ccttatgaag gggattatgc aatcgtgaag cggttgattg
     5281 aagaataatg gtatttatat actagtggtt tcccatttct aagaaaaaaa gggactgtga
     5341 ttttttgtca cagttccttt tttcttagaa atcattttaa cttttcacta gctaattctg
     5401 ctgccttttc tctttcttca tttaataatt tattatcata gaatttgata aatgggaacc
     5461 agataatgaa tgcgatgatt gcacaaatga ctgctaacac agcagctttc caatcaccac
     5521 cactaccaac aaatgctccg atcccgactg gtgaaggcca cggcatctgt gcaagcatcg
     5581 gtttgacgat ttccagccga atggcaaaat aagcgagtgt agcagaagcc atcggagcta
     5641 agaaaaacgg aaccgctaaa tagggattgt agacgattgg catcccgaaa atgattggtt
     5701 cattgatatt gaataatcct ggtacaagtg aggcttttcc taacactttc agctgatcag
     5761 atttcgcgag gaatgcaata aagatgatca atcctaaagt cgcacctgat ccaccaacgg
     5821 ttacataaga attgttgaat tctcctgcca acggataatt tgcgccttgt gcgttagctg
     5881 ccatattagc taatacgatc ggggtcaaga aagaagtgat gatattcgct ccatggatcc
     5941 ctacgatcca caatgcatgg atcaagaaat aaatcaccat gacaccaagc catgtattcg
     6001 tcaactgcgt aacaaaccca aatgggatag caatcatttt gaagatatcc gttcctagtg
     6061 caactaataa tccattgatg atcatcacca caaacgcaat gacaaatgtt gggatcagag
     6121 cagtgaaaga acgagaaact cctggcggaa caacatctgg catttttatg acccaatttc
     6181 tcttgacaca catacaatac aattcgaccg caataatcga cataataata gctgtaaaga
     6241 ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct aataccattt actacagttt
     6301 ctgtctcatt gatagaagca atcaagctga ttttaccatc agtcatgatc aattcaggga
     6361 tacacataaa gaatgcaaaa acagatagta atgcccccgt gagcggatta acatttaatt
     6421 tttgttcatc tcgttcaatt gacgttaatt cataagcaaa cacaatggca aagtaaatag
     6481 ataaaatccc catcgtcatt gtattgacga tcatgtacaa gtcactaact ttgaaaaaag
     6541 ttgcattaaa aaatccttct aaaaatggga acggtaatgg caaaatattt aaaaccaaaa
     6601 acatcgagcc tacaatcgtg aaaggaatag aagccatccc cgctgccatg acagcccgaa
     6661 cgattttaag ttgcgcaatt ttccccatag gtcccatcag gtatttctgc aaaaaatcaa
     6721 acattagtta ttctcctttc ctaaatataa cgctttttca tattgtttaa tacggatata
     6781 ctgttggtct tggttgagtg aaattttctt taaacgatac tgtataaatg cactggcaaa
     6841 aataccgctt acaacaagaa tgaaaacaaa taactgtcct ccattgttat ttgccataaa
     6901 aggatacaag aatgtaaatc ctgaggtaaa caacactgga atcactaata aattaaagac
     6961 actctggaac aaaaaatacc actttgtcca tggaacgttc gtttggtgtt ttcgataaag
     7021 ctttacttgt tctcctactg caggcaaaat agctaaaatc agaatcatcg gtaaactact
     7081 tcctaaactt ttagctgata gaagcagaat catccattgc atgttcatga aaaagaaaaa
     7141 ggcagtcagg taacgtacga ttagataacg attgaaatac atgtttttca aactaagttg
     7201 ctttttttct aattcgattt tcctttttac tgtatcctgc acaatgatcc ttcctttcta
     7261 tttatttttt tgatacaaat aattcatttc ctgtgcaact tccaataatg tcatcgtcgt
     7321 catcagatga tcttgtgcat ggaccatgat aatttccatt tctatttttg tcccgctggc
     7381 atattctttc agaagttgag tttgtgactg atgcgcatga agaatctctt catttgcttg
     7441 tcgcaacttc tcttctactt cttcaaacat ctctttacgc atcgagcgaa atgcttcatg
     7501 tatcagtgtc cgtgcatttc cgctatggaa aatgatatca aaagaagtca cttgtagtgt
     7561 ttcactgttt attttttcag ccatctgttt tcctccttat tcgatcagtt tgataaattt
     7621 actgctgaaa ttctcaaaaa taggttcagc taagatttcc tgctgtactt ttagctggtc
     7681 aactaaccta acgattgctt tagtcacgac cgtaattcct tcattttcga cataagaagg
     7741 cgaaactaaa aaaacaaaat gaatttcttg atattgatcc cactgcatcc cagaaggaat
     7801 caatgcaacg gctacctttg tttcgattcc aactggaaca gcgggatgtg ggactgccac
     7861 tgtatcactg aatatgattt gtcccatatt ttcacgttgc tggatctgct ttcgcatttc
     7921 tcttatatat ccttcttctt catttatgct cagcttttgc agcaaatcat gaagaatttc
     7981 ttcctttttc ggttcgtacg tataagtctt gaaaaatttt tcagacaact gttcggtgta
     8041 aatctgcttt ttttgctgta gagaaaatgc tggtatcggt ctatctgagg aatgataggg
     8101 tatagattct ccaactacct ttcgtatttt acgaacatca tcttcattca aaaaaatact
     8161 gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag
     8221 gtcgattcct tttaaagttg tttcattgat ctcataatag ccttttacac ttaccactgt
     8281 aatattttta ccgaattcac tgactacgcg attctttaat agttgggcac tgccgtaacc
     8341 cgttgcacag ataatcaatg catgtagttt atgcgcagct ctttcttttt ccaatgccgc
     8401 catgagatgc aaagccaaat aggcccattc atcttcattg atggtatagt ttttcaaagc
     8461 cggcatgttg ctgaaatgcc tttttactgc gtcaaagact ttgggatttt ctttctttat
     8521 ctcctcagtc aatgggttct caatgcgaat acctcggctc aatcgaatca tcatcggtga
     8581 caaatgatcg agtagtccat tgaaaagctg gagatcctct actaatgaaa aatgaaattc
     8641 ctgagacaat cttttcagaa ctttcgctaa ttcctttgat agttcctgat cattttgatt
     8701 ttctttgtga ttcgacttag ccatgagatg taatgtcaaa taagcaattt cttcttcggg
     8761 aaacgataca cctgcgatta cctcaatacg ttgaacgatc cgctgcgcca cttgatactc
     8821 tactcgctta gaaacttctt gttcgatgcc taattccttg atttcaaacc cttctcggag
     8881 ccgtttgatc cccaaagcca agtgtaaagt cagattctgt atgataaaat ccgaaacttt
     8941 taacttagct tctcttgttt catcgagaat gataatgact agctcttcaa aactgatgtc
     9001 tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa tttccataat ggtttccaaa
     9061 aaaagtatcc atgataaaat gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg
     9121 accatgctta gaaacgattt ttagtgaata tggaatcaat cgctccttga tttcttgcat
     9181 gtcctttgtc aaactggaac gactgatata aagctcttct gccaaatcat ccataaaaag
     9241 aatggcatct tccaaaagca attggttcaa tatatacttt tgtcgatctt cggactcata
     9301 aaatccttgc ggttctctag catatccagg tactatttct tgccgactca aaaacaagtc
     9361 aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa cctggtttag caatgatctt
     9421 acctccgttt ttttctacta actctttgat ccgctgcaag taattacgga ttgtccgatc
     9481 agataataac aattcagaag ctaaacgttg actggtgaca tactccttct tgtgatcaat
     9541 caaatatctc aacagcaatt tttccttctg tttcattatc atccactcct attccatcat
     9601 atgttctctt cgatcacctc tgctaacttc tggatgcctg tagggattgg gacatacgct
     9661 tggaaaggga tactaacaac aggctttcca acagattctc ctaatttttt gaatttatcc
     9721 aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa aatcactatt cttaatcata
     9781 ttatcgcctt ctgtagctga aactgcatct acgagtacct cttctctttt ttgcttaaag
     9841 aaatccgttg tctttgaggc catcatcgag gatgacattc ctgccgcaca aataatcaat
     9901 gcttttttca taaatcaatc actcctttta tttttataaa ccaagcatat cttgtaagcg
     9961 ttttctattc aaaatatata tttccgtagc attgcggaaa aaatgtgtat catatcttga
    10021 ataataattt ctggcaccgt gatatactag tgatagaatt ttttcaggag gggatttagt
    10081 gagtatcaca gtgaatattt tgtatacaag tacaaatgga caagccagaa aatttgcaga
    10141 agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata
    10201 tgactattac tttccactag aagatccaga atctctttta ctgatcgacc gttggaaaga
    10261 tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg gctcaaataa acgaactccg
    10321 taaaaagtat aaattaaaaa tgaaagtcga acgtttcact gatttaccgt aaaaagatac
    10381 acatggaatg atagcaattt atcatccatg tgtatcttca ctttttcctt attttttatt
    10441 cgatctcatc taatgattca aaccgtgttt ttctcttatt tttcggataa gtatttcagt
    10501 caccccaata tacatgattc ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt
    10561 tactgctcct ggaaccccga ataaactggc agtgattgcc aaagcaccaa aagaattaaa
    10621 aactgtttta tatctatagg tagaacaaac acctaaagct agaccaccta atataccgac
    10681 ccagttaaaa ggaataaatt ggctgatacc aataaataaa acacatccaa tcaatgagcc
    10741 aatgattcgg tctactgctc gttctttgaa accaatcgac atcaatccat agctagatac
    10801 aatagaagaa aatgcaaacc ctgcccacat caatcgctga aaagggacat actctcctgc
    10861 aacgatcaaa agactaatgc caaatgcata gatgagcatc caaatttttt cctgcgaata
    10921 catgcctttc aaaaaaatct ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt
    10981 tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat aataagctac ttgtttgtgt
    11041 aaagaaattc ttgttcaacg aatctttagg taacgaataa acaataaaga gatatgaaaa
    11101 gccgtacaaa gaagcattcc ccattttggg atcactagcg gttgccatta atatgctgct
    11161 tagtaaaata aaatgaatca acatgcccat aactgaccat ttgatcagct ggacatacgg
    11221 tgctactaat agtgagaaca tgacgattgc taaacctatg atcgaatggg aaatcttata
    11281 accaaagtca acaaaacgaa tactcatcaa catacagaat aaaaccacag cgtatggttt
    11341 atgcttcgat tcaaataatg ttgtaattat aatgataaaa caaatggcaa acaatacgat
    11401 gaataatgat cgtaaaaaca atgaacaaat atacatgcac ttttcttttt tagaagctgc
    11461 ttctcgtatt ttttgtttta aaataaacgg atctagttgt agagactgat aaaaatccac
    11521 ctgttgtcgt cctttctttt tacttttatt ttttacagtc atctatgaca ggttgtaaat
    11581 actatggaac atcatatggc gttgtcattt tcttgtcaac tatttttcct attcatgttt
    11641 ttataaaaaa acatatcgtt ttcttgtatg attaaatcac cacaaaataa acaactgaga
    11701 gaacgatatg ctttatgaat ggttctatca aaaaaattct gagatcaata gacaaaaatt
    11761 tgatgattac agaggtctct tacgaaacct tacagaagaa aaaaacattg atcgttgctg
    11821 cagttctctt ccctgctcca tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa
    11881 ataggaaatt aatggtggtg aaaaacaaga agaaggaaac cattgtccac tttgaataat
    11941 ataatcatat gccaatggtt atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc
    12001 gaagccattg gaacgctcaa agttatttta tccgtccaag gcactcgatt tcaaatcatg
    12061 tcagacataa aatcacttct ttactgactg aaaaagtgtc gttatttttt atttcgaaaa
    12121 gttgttaggt atctttgaca accgttattc gcacattgaa agagttgaga agctatttac
    12181 caaagtcttc caaaagaatt ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt
    12241 ttccgttgag gataaactga gttttatttc cgcggatggc gagacagaaa aattaatcga
    12301 gaaaatttac ctacacaaaa aattcctcga ttaacaaact atttccttag ctgcactaat
    12361 cctaaagaaa tagaattctt agttacagac atgaacgcca cctactttca gctcaccaaa
    12421 catgttctac caaatgcgaa attggtgatt gatcgattac ttggtttttc cacgcctttc
    12481 aaggaggcgt aacctttatt cacgagttgg ttgaagcttt ccgagacaaa agacccgact
    12541 tgtttttctc cttattgaca aagagccatt aaaactagag aaaaaattac aactgatcgc
    12601 tgattatttg tagaaagaaa atcaaagtat tacccaataa acttgagtca ttaggcaaaa
    12661 aacactgcga aactcaagaa tgattttcgc agtgtttttg tatatttttt attaactagt
    12721 attactacaa aggtttattt ttcacttctc aacgcagcac aaaattgtct ttacttattt
    12781 actggttggg ttcaaccttc tctactccga tactaatatt tgtttgtata ccccttctaa
    12841 aatcggtagt ttccattcaa ttgatgggta ctagttgatt ttaaaaacta tgcttattca
    12901 attttttatt ttagctcata tatttcgttt ctttctaaat tttatactga tgaaaacaag
    12961 tagcacaccc aatattgaca atataaactg gctaatgatg gtttctccag tttttggaag
    13021 atgcttattt ccactgcttg ttggattgtt tcctcttata tctgagctat cagcaacttt
    13081 tctattctct tttgttaaaa agatcgccac ttcttgttct ttttcgatct tgaaagttac
    13141 tggtgtcgca tccaattcat accccgttgg cgcttgagtt tctaccagct gatatgctcc
    13201 tggtgctaag ccatctatcg ccagttttcc atctgctcct gtggttagac ctgactgcag
    13261 aacggttcca ttggcatctt gtaactcaaa aacagctcct tgcaggactt ctccgctttg
    13321 gtcatcgatc tttgtcaaca cgacaccgcc tggtgtaagt cgattttctt tcgtcaattc
    13381 taccaccgct gtctgacttc tttcgatctc aaactcgatt ggtgtcgcat ccaattcata
    13441 ccccgttggc gcttgggttt ctaccagctg atacactcct ggtgctaagc cgtcaatcgc
    13501 cagttttcca tctgctcctg tggttagacc tgactgcaga acggttccat tggcatcttg
    13561 taactcaaaa acagctcctt gcaggacttc cccgctttgg tcatcgatct ttgtcaacac
    13621 gacaccgcct ggtgtaagtc ggtttgcttt cgtcaattct accaccgctg tctgacttct
    13681 ttcgatctca aactcgatcg gtgtcgcatc taattcatac ccc
//
LOCUS       DACDIP010000063        13701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDIP010000063 DACDIP010000000
VERSION     DACDIP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.063"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(50..874)
                     /gene="iolB"
                     /locus_tag="IV257_002182"
     CDS             complement(50..874)
                     /gene="iolB"
                     /locus_tag="IV257_002182"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP8815279.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(913..2829)
                     /gene="iolD"
                     /locus_tag="IV257_002183"
     CDS             complement(913..2829)
                     /gene="iolD"
                     /locus_tag="IV257_002183"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP8815280.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2840..3874)
                     /gene="iolC"
                     /locus_tag="IV257_002184"
     CDS             complement(2840..3874)
                     /gene="iolC"
                     /locus_tag="IV257_002184"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP8815281.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4031..5047)
                     /locus_tag="IV257_002185"
     CDS             complement(4031..5047)
                     /locus_tag="IV257_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8815282.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5149..5346)
                     /locus_tag="IV257_002186"
     CDS             complement(5149..5346)
                     /locus_tag="IV257_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815283.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5318..6637)
                     /locus_tag="IV257_002187"
     CDS             complement(5318..6637)
                     /locus_tag="IV257_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP8815284.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7378..8358)
                     /locus_tag="IV257_002188"
     CDS             complement(7378..8358)
                     /locus_tag="IV257_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8815285.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8371..9249)
                     /locus_tag="IV257_002189"
     CDS             complement(8371..9249)
                     /locus_tag="IV257_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8815286.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9265..10065)
                     /locus_tag="IV257_002190"
     CDS             complement(9265..10065)
                     /locus_tag="IV257_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP8815287.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            11457..11783
                     /locus_tag="IV257_002191"
     CDS             11457..11783
                     /locus_tag="IV257_002191"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8815288.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11791..12699
                     /locus_tag="IV257_002192"
     CDS             11791..12699
                     /locus_tag="IV257_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8815289.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13328..13579
                     /locus_tag="IV257_002193"
     CDS             13328..13579
                     /locus_tag="IV257_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP8815290.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
BASE COUNT         3868 a         3049 c         2325 g         4459 t
ORIGIN      
        1 aaaacctaac taaacctaac taaactttat gtatctaaca gcttaataac tacttgcgct
       61 gcaagatcca ttcgtgtttc ttttcattgt ggaatttcca aattcgctca ggtcccgcca
      121 tcacatttaa atagtaggaa gtatacccgt caggtacccc aactggatga tagcccttag
      181 gaacttcgac gacattttga tgctctaccg tcatcgtttc atctagtgaa cgatcttccg
      241 tgtagacccg ctggaaaaca aatccctgct ctggatcaag ctcatggtaa taagtttcct
      301 ctaaaaaaga ctcatgtggc agattctctt gatcgtgctt atgaggagga tagctggaaa
      361 aattaccgcc ctcggtataa acttcgacga ccagcaggct gctggcagta tcgacattat
      421 ccggtaaaat gttgtgaaca agtcgtttat tctgatagag tccacgttct tcgacggtat
      481 tatcctccgc gcgaatcaca cttatcggca aagctttttt cgctggtgaa tagcaaagcg
      541 ccaatcggac attgtctgta gttgccacaa gttcaaacga atgctccgcc ggaagataaa
      601 tactgtcggt tggaattttt tcaaaaacag attctcgtgt tcctaaatct tcataccaag
      661 tgtctgctgc tttaacctta atttttccag tcaaaatgac gatacagacc tcctgttgac
      721 tgccggtttc cttgtaagaa gctccctttt ccagctccaa aatctgaaat tcgatataac
      781 tcaaagcaga attagactgc cgaatatttt ggatttgttt tactccaggt gccagttctt
      841 tgtttaatgg ttgatgcttc aatgtctttt ccaatcaatc cactccttat tttcaatcac
      901 aaaaagcaat atttaatact ttctgatagt ggcaagtttc gcttgtttgg ctttgtaagc
      961 ctcctgcatg gctgctttac ccgatacctc tgagataccg acgttccacc agctgccgtc
     1021 gtagccttct gtcatggttt taggaagtac ctttatctca atcaacgtcg atcgcgtttg
     1081 cttcttcgca tcctcgaccg ctgcttgtaa ttctgcaatc gagcttacac gatacgtttt
     1141 tgcaccatag ccttcagcaa ctttggcgta atcgatgttc atgatctcgt ccttggtatt
     1201 tctgaattcg gttccttggc tagctccgcc attatccatc tgtaaattat tgatacagcc
     1261 gaaaccagaa ttatcgaaaa gtacaatgtt gattttttta tcatattgca aactagaaac
     1321 cagctcgcta tgcaacatca agaaactgcc atcgcctact aaggcataga cctcttgttc
     1381 tggtcgagcc attcttacgc ccatcgcacc agcgatttca tagcccatac aagaataacc
     1441 gtattccaaa tgataggtat tctccttcac agggttccat aagcgctgca tatcccccgg
     1501 caatgaaccg gctgaaccga tcacgatcgc atcctcatca acaaaatcat ttaaatgaat
     1561 gaagacctct gtttgcgtca aattcgtagc caacgcatca gcatattcat tcaagatttc
     1621 ttgagaaaaa tgatctttga tttctggcgt aaaatcttta cgcttaaaat gcgcggcttt
     1681 taaccgattt cgctcactga tccattcttc cttcagttca tcgatcttct cgccataggc
     1741 ggtttggtaa ccttcgatca ttggggcaag ctgctccaag gtcgctttcg catcaccaac
     1801 cacttgaaag gcttccagct tagcggcctg ttgtctgctt acgttaatat tcatcatttt
     1861 cgcttgttca aaattaaaaa tacttttaga agctgaggta aaatcagtgt agcgtgtgcc
     1921 gatccctatg atcagatccg cttgctgcac agctttatta gcagccgaag ttcccaaaat
     1981 ccccattccg ccgagattgt ttttaaaatc ccacgaaaca gtcgatttac ctgcgtgcgt
     2041 ttcaactagc ggaatctgac aatctcttga gatttgctca agaatctcac cagcctccga
     2101 atatttgcag ccgcctccta cgatgatgac aggtcgttta ctggccttta ttcgttgact
     2161 cgcttcagca agctcacgag cggtcggaat cctgcgatca atataatgta ctcttttttt
     2221 gaaaaattcc tccggatagg cataggcttc tgcctccacg tcctgcggca aacaaatcgt
     2281 tgccggaccc atcgttgccg ggttcgtcat gacttcaaag gcccgcaata acgcggacat
     2341 caactgctct ggtcgattga tgcggtccca gtagcgagaa accgcttgaa atgcatcatt
     2401 tgtcgaaaag gctacactgc ttgcatgttc cagctgctgc aagaccggat caggctgacg
     2461 tgtagcaaac gtatccgccg gtaaaaaaag tacggggata ttatttgcga atgccgtacc
     2521 cgctgctgtt aacagattgg ccgcgcccgg tccaacagat gttgatacgg caaaaatttt
     2581 cttgcgtaaa ttctgtctgg caaaggcagt agccgcatgg gccattccct gctcattttt
     2641 tccgctataa cttttcaaat gtccgggctc taattccagc gcctgaccga tccccaaaac
     2701 atttccgtga ccatagacat gaaagatacc ttcaacataa ggcgtttctt tgccgtcgat
     2761 gctgatgtat tgttgattca aaaattttag taatgcttgt gctgttgtta aacaaaccgt
     2821 cttttccatt tttttcacct taaatccgag cgatcatttc gccgtatatt tctttctcca
     2881 atgtaataaa atcttttacc tcctgagtcg tcggtaaaga atctgaacag ttattgcttt
     2941 tgaccatcat tgatgcttcc gctgagccaa actctaaaca atcgatgatc tcccagcctt
     3001 cgaataaacc gtataagaaa cccgagccgt aaccatctcc gccgccaaag cctttgcgtg
     3061 cttccaccgg aaacggcttg attgaaaact gctgctgatc ggccgtataa gcagtcgatc
     3121 ctttcattcc atgtttgatt acgacaatct cagcaccaca actgtgccaa aaagcagcgc
     3181 tttcattatc tgacatgtta ggctggatca acttctcagt tagatcgaac tcttctcttg
     3241 aacccatgat aatgtctgat tccttagcaa tcatcgaata gtaaattgaa atatcatcgt
     3301 tgttcttcca gttataagca cggtaatcaa tatcaaaaat aattttgaca ttattccttt
     3361 tcgccagcat cactgccttg attacggctt ctctggacgg gctctctgct agtgcagttc
     3421 ctgagattaa tagtgctttt gaccgtttca agtagtcctc atcaatatcc tcaacgctaa
     3481 gctgcaggtc tgcggcttga tttcgataca tcaaaatatt actttccttt gatgagagca
     3541 tttcggtaaa agtcagcccc agcttttcac catgctgcgc tttgctgatc tgagacgtat
     3601 cgatgccttg ttcattgaaa taatcgatca catattcgcc aaactgatcg tccgagactg
     3661 ttccgataaa accagctttc atgccatgtt tagtaacacc cacagcaata ttggcaggag
     3721 atccgcccac aaatttttca aacatatgga cctttttcaa tggtttaaac tcttctttga
     3781 cttgatcatt gtaggcagga ttaaaatcaa tagcgactct tcctaaaagc agcaagtcaa
     3841 attcttttga ttggtcaaac tttatataac tcaatatatc cacctcttta tccgttcttc
     3901 tatttctgag gcaagtatag cacgccaaaa caaagcgtgc aatatttatt ttagaacgca
     3961 atattgcgtg ctgtttgaaa atgacggcaa aaaaaacgtg aatcgcctta catcacgatt
     4021 cacgcctaga ttaaatattg tattttgtct taataattat atcagtaaat agcgcatctt
     4081 ttgtaaccgt tttattattt tgcagctgat tcgctaacat cagtaatgaa cgatagcctt
     4141 gaacaaatcc atcttgatcg attagaaaat caacctgatc gtttttcagc atctcgatat
     4201 tgttcttagt caaatcataa atgattacaa agggccgctt gctttcatcg attttttcca
     4261 acgctcgggc aattcccgct tgtccgccag aaaagacgac tacgccatct aagtttgaat
     4321 aattctccaa ggtgttgctt aaaatctttt caacctcagc actcgaatca aagctgctct
     4381 gaacacccgt cagctccagt tcaggaaacg actctttcaa ttcttcaaca aagcctgcca
     4441 cccgtaagct gttgacgcta tttccaaagt agccggtaat cgctagaacc ttgccctttc
     4501 cttttgtcag catacctaac aatcccgcag ccgtttttcc gcttttgaag ttgtccatcc
     4561 caacaaaaca ctgtcgattc gtaccaagaa tatccgaatt aaaggtaatg atgaggatgc
     4621 cttgatcggt caattgatta attttttctc tgataccatc actatctact ggcatgatcg
     4681 cgatcccttt tactccaagc tccaccaatc gatccaacgc ctgcagctgg gccgcttcat
     4741 cgaccccgtc tacctcttct aaaaaacaat caatgtttcg cttttttaaa tccccgataa
     4801 tagattccag acctgatcgg atcggaatca taaaagaaga tttcgccaat tgagtgacaa
     4861 tccccagctt gagcgttgtc tcttttatga ccttcttttt ctttggaaca tagttcattt
     4921 ccttggcgat tgctctgatt ctttctgcaa cctcaggatt gatccttcct cgatcgttca
     4981 atgcccgatc cactgtcccc cttgacactc ctgctttctc agctatttct ttaattgtac
     5041 ccgccatctt gcctctcctt ttttcctatt gtgtttattt tatcacaaaa cttaagaaaa
     5101 ccaccggaga atgaattctc cggtggactt tttgtatgat taaccttctt attgaacatc
     5161 actgatctta aactctattc caagggcgtt tctataatat tctggataca tctccttccc
     5221 acatttttca caggtaaatt ggggaggatt gttcggatca tttgcaatat ctgcatcatc
     5281 taaaaatttt actacatcca ttgggattga ttctttttca ccacaactta gacatataaa
     5341 tttcacgttg gcgttcccaa tcccagcgaa ttgctttaaa agttttttct ttttcttctt
     5401 catactgttg cctgaattct ttttggtcaa tttttcgatt ctcctctcgc ataaaccgct
     5461 ttgcatggtt atctttcgca tatttaataa ctaaccgtcc atttttgcgc acactcatcc
     5521 caacgaattc atagtactca ccgcaacaag aacattctaa gggattttca ccaaattctt
     5581 cggttattct ttcttcccag gattttggtc ttaaggcctc attcagattg ataaacagct
     5641 tttttactcg tttctgatac tccttcatga cctttttcat taatgttttg attctacgac
     5701 tatagattcc atagcgacga atcgttttga aattcttatt ttgaatatgg cgaatcagtg
     5761 cagctatgaa ttcgtctata ctcatggtca tcgtctcttt agtattcgtt cgtttgtcca
     5821 tgtagttgaa catgacagtg tcgccatcat acattaagat tcggcttaat gcgatcgggc
     5881 cacgcttcat ataacgactg atgtattgga gtagtgactt tagatttgtc ctgctccttt
     5941 taggagcatt tacgtaaaac ccttctccat tgttcttgta cgcagcttgt aatcttgatt
     6001 gaactctttg cttgtcccac tcacctaacg ttcgtctaat tagtttcaat acagcatttt
     6061 tccagtattt tcttaacata gaaaaaggta aataatcgta ttcttcccaa cttccatctc
     6121 ttgttaagcc acccatagta acaaccatat gaacatgcgg attaaattct agttttgatc
     6181 caaaggtgtg gattgtagct actatacctg ccttcacctt tcttttctca aaataggcaa
     6241 gaataatttt tgcggcttca tccattagtc ctttcagtaa ggaatctcga tagttttcca
     6301 tcataaagat ttcccgtaac ccctcatcga tcgtaaagat tacgtgccga tgtgtcactt
     6361 taaacatatc attcgccaca accgctgacc acctttggct ttcacctgtt gcacacgttg
     6421 gacagaactt tccacgacat ttcaagggaa caaactttgt ttgatgacat ccctcgcaga
     6481 caaacagtcg gtatcctttt tgaatatctc cacaatatct aaacttgttt acttcccgtt
     6541 tgacgactag ccgaatcttt cgcccgtatt tctcaacaaa gttatcccaa ttatggtgtt
     6601 cgtcaaaaaa gattgcttcg agaatattcg ctttcatagt aatattttac ttgttcaaaa
     6661 acaaaatgaa aagggctaaa agactggtag tgcagccttt ttagccctta actttttata
     6721 ctttctttac tgtctaaaaa atatgcggcg cacagaacgc gccgcatatc ggactaagtt
     6781 ttattcaaat ttgtcttcgc caacacaatt tgacgaagag ccttttcttt caggaagact
     6841 tcggtaattt ttaggagttc tacctattct tttaaccgtt tattctcttt ttctaagcgt
     6901 gtaatttcat ttctgcgaat ggatttcatg acttcctcta ccgagaaaag tacgttatcc
     6961 atatttttca acttcttgaa ctcatcaata cacgctatta gagcgagctt ctagctatag
     7021 actaacttct ttaacagaaa acccttcttc cgttaccagt tttacagcgg taattttaaa
     7081 tatttcatca aatgaacgtc ttggcataat ccagatcttc gctttctctt aatttggtgt
     7141 caattttagt ataacccgtc caacttattt tttaactaag tgcccggttt ttcttgacaa
     7201 ctccacccaa aagttagatt ttttggtcta agttttaggg tcgcttcatt actagaattc
     7261 aacaagagat gttttttatt ttatttaaat tatggggtca gtttccattt gattacaaac
     7321 ttctttgttt tcttttttta aacgcaataa taaaggtttt aatttcagcc tttttaatta
     7381 gaaaatcaat tttcccaaat tattttcttt agctttctta acgatttctg tcgcaactac
     7441 cacatctaat acagccgaac caactgtttt gaagatcgtg atttgttctc cacttgtccg
     7501 accgatcaat tcttcattga tcagttggcc tagttcccca tgatagctgt ctctttgaat
     7561 atacccatct tgcagcggtg aaatgaagtc cccagcttct tttagaacac cgtccatggt
     7621 atcaaaaata acaacatctg cggctttgat tatttctcgt ggaatttcac acatttccgg
     7681 tgtgtaagca ccgactccat tgatgtgcgc accttttttg acccactcag cagagaatgt
     7741 cgcacgtttt gaagtggtca cacttgtaat gatgtctgct ccttcaacgc attcttgact
     7801 ggtttttgtt gggcgcatcg ttacagaaaa ttgttgcatc attttttcag caaattggct
     7861 ggcacgctca aaatcaatat caaaaatccg aacttcttct aatttacgaa cggtcaacat
     7921 tgcttctaat tgtgactgtg cttgtccgcc ggttccaatc agcgcgccaa ttttagcatc
     7981 ttctcttgca agaagttctg ttgctgctcc ttgaacagcg cctgttctta attgtgtcaa
     8041 ataagtacca tccaaacaag cggataccat acccgtttct ggatccaata cgatcatcgt
     8101 tgctggtaca cttggtaagt ttttcttgat gttttcagga tacacggaaa cgattttgac
     8161 acctagcgcg ccttcgccac cttctacata agcaggcata tataagcttt gtccgttgct
     8221 ttttggcaca tcgatattgg tgcgaagagg cacacttgcc tttcctgctg aatacaatga
     8281 cagtgctttt ttgtccgcat cgatggcttc tctcattgaa aaacttttga taatatcttc
     8341 ttttttaaga aataacattt gaatcgctcc ttaattagct gctgtaactt tttttaattg
     8401 actgcccaac atcgtatatg cactgtatag taagaccaaa atcacgatcc atttcaggat
     8461 ataggtatct aaagatttga ctaggaatac agcgaccaat acccccaata caccgaaggt
     8521 tgaagtaaaa agtgtgattt ttctgctgta ttcattaaat ttaatgaatt gaacactgcc
     8581 gatcggtact gagaaggtac aagcccccat cataatagga aaggctgcaa ctggattcaa
     8641 gcctagcaag tagacagtta ccatcgttaa cgcataagaa ccaatgccga tattgttcaa
     8701 tgcaccatag acaaagagta aaaatcctgc caagattaat tttccgccat acaattcact
     8761 ggctgttccg tttgatggaa tccagttcat ttgtcctgca aaaatcaaca atgcagcaat
     8821 gaccaatcct atgccgacaa acagtttgat cgttttctct ggtaacttca cgacaaatct
     8881 tggtcctaaa tatgcaccga taatttggca gataatacaa acagctagtg tcttgatccc
     8941 tacgttgatg gcagtgatgt aagacaatgc catgacggct accggtacta cacattgggt
     9001 attcaacgtt ccgggtagtt ttttcatgct gacccatttc aactttggat aaaggacggt
     9061 tccgatagca aagtcggaaa cgccaaatgt tgaaagaaag aagatgataa atgacgtgaa
     9121 gggcaacaga cgcatatccc ctggttcttc tttgaaattt tgtttgtttt tgaaaaggtc
     9181 tcttagaaag taaataataa aatatagatt gattgcgaca attagtgtca gcaaaatatt
     9241 ccgaatcata aaaaattcct cctactagta aatctttctg attccctcaa agccctcacc
     9301 atattttgct ttaagttctt caggagattc tgcaatcact tttagtgtat atgcatcatg
     9361 tttgacatat tcttttgcta gattgaccat ggcgtggttt tttgtccaag tgacatcggt
     9421 tgattgcagt tcttcgttaa tggtaccaaa cacgacttct tgctcatcgg cgacgatgtt
     9481 gatccatctt gctccaaagt cagcaagttt gtctgtttca aaaccatgaa tataatactt
     9541 ttctaaggga agatcatatt catgggtaga actgaataaa atcagtacaa acttatcaac
     9601 tcttttttca gcttgctgca atgcctttag tagtgaatcc gtgaaattct catgccaaac
     9661 ttgaatgagc aaactttctt ttgcgttttt caccaaatgc tctactttat ccagaacata
     9721 ctcgatacca tcaactttcc acagcatttc ttcttcatct ttttctttga tcatccctag
     9781 attcttggta agaaaagaaa gatcattttt aacacttttt tctaatttct ctaaaaactc
     9841 atttgccgag atggcatgat aaagtttagg ttctgattta ttaacgacaa tcaaattctt
     9901 ttttaatagt ttttccaaaa tattatatac tttcgagcgt ggcacacctg agattttact
     9961 tacttcatat cctgtaagat tacctttttc taatagtgtg gtatacactc ttgtctccat
    10021 ctctgaaaaa tcatattttt tcatgatttg aataatcgtt tccattgatt aatcctcact
    10081 ttatttttag tagctactac ggtaacaact ataaattctt tttgattatc tgtcaagagt
    10141 tagttcaata acacacatat aataattagc ttaaaaacaa tctttttatt aaaaagaaac
    10201 attataatat gaatgtttgt gaaaaaataa tacaacttag tcattcttta caacgattta
    10261 tagaaaaaca acctctttaa atataaaagg tacgggttac tgattacttg taaatcttta
    10321 cgttccagtt ttattctttc taaaaaatat actcacttct tttcttagac ttcttttttt
    10381 gattctcctc tttatggtct tgtccttttt tgccttcaaa atatttaaat aaaccccttt
    10441 caaaagcatt tctaggtttt ggacaataag gaatgacttt tttcttcata taaatacctc
    10501 atttaactat ttgatatcat cacattatca aaaaaaatag gttttttatt aaaactatct
    10561 tgacctttcg ggagatttca ctgtgattta tttttattcc catacttttt tcgttggtca
    10621 ctttcgtata cctaatctaa gcgatcatcg gcgacggtgc ttttacaacg aatggcagga
    10681 caaaacaagg ggcggaaaaa cttttttgcc tatttcataa aaaacttttt cctacagcaa
    10741 tcccttgttt tgtcctacca cacgttgtga acccaaagca cgccgacgaa cgcttagcta
    10801 tacgaaaatg acctccaaaa acaaaaaagt gaggtgaata gaaacaaaaa gaaattgaaa
    10861 tctcccaaaa tgtcgtccgc ctcggagaca acttgctgct gaaaatggtt ggttaattca
    10921 tttaagtatt aaataagata aatcatcaaa ttaatcggaa aaatagcatg attggcctta
    10981 tcaaaaataa actgtaaaaa cttatatttc tatgagtggt ggaacaatgc taataccaaa
    11041 ttaatttatc gattacccac ctgcacaaac caaagatagc attattagga aacgaatcaa
    11101 tcagcgaatc cagcattata atgaggatac taaaaataaa gttttcataa ttataaatcc
    11161 gccttcctac gaaaggcacc aatgaggtga tgaaaacctt agagcgcttt ctttcttttt
    11221 ttattccgtg ttaccctttc cttgcaggtc cctggtacac tacagaaccc gaggaggtga
    11281 gtggcggggc tgtatagcgg caaccccgca taattatatg gtgtcggtca tccgttaagg
    11341 catcaatgaa tggcgcaaac ccgtagcccg taaacttatc tcttccgaag ttgactcgat
    11401 ttcgccggat gaccagtccc taccaaacct gtactatttt tttacaggag gttgctatgt
    11461 tcaaaatctt tcgtaaaaat tgttgtggct tggatgtcca caaaacgtgg atctatgcgt
    11521 gtatcggaat cacagatgcg aatggtcgta cagtgtataa gcaagctcgc ttctcttcct
    11581 ttacaaaagg tctcaatgaa ttagctgaat ggcttgctaa atacaactgt aaggatgtgt
    11641 gtttggaatc ttccggtaaa ttttggattc ctgtttttaa tattcttgaa aaaacttgtt
    11701 ttgtcacact ggctcatccg aagtacacca aaccccagaa aggaaataaa acagatcgca
    11761 aagatgcaaa aatggatttg tgatctcttt atgtgcgaca tgatcaaacc cagttttatt
    11821 cctggtcttg aaatccgcca gcttcgtgat ctaatgcgtt atcggacgaa actgaccaac
    11881 atgaaaacaa gtgaaaagaa tcgtgcttta aactgcttaa ccgtttctaa tctgaaacta
    11941 gatgatgttt ttagtgatgt gttcggaaaa tcatccactt ctatcatcca atacttgtta
    12001 gaacatcccg gtgaatcatt tgatgttcga ccatttatcc atgcccgttg taaaactccc
    12061 gttgaagaaa tccaagcggc agttgagggg gcgatttctg tacctcaagc gattaagctt
    12121 cgccaatgcc tcaaccatat taacgagatt gagaagcatc gagaagagat caatctagag
    12181 attcaagctc ttgcagaacc cttctcccct gttttggaac tacttcgtac cataccgggc
    12241 ttagacactc agccaataac agcgattgcg attctttcag aaataggacc ggatatgtcc
    12301 gtgtttccaa cttcgaaaca ctttatttct tgggctggat gctgtccaag aaacgatcgc
    12361 agtgcccgac gaacaaaatc aacacgtatc tctcgtgcag ggcagtcgtt gaaaccgcta
    12421 cttgtacaga ttgccaatgc tcttctcaga tccaaagaac atccagaatt taaaaatcgc
    12481 tatcgtagaa tcaaagcgaa tcgtggacac aagaaagcta tcattgctat ttgtaagatg
    12541 ctcctgaccg ctatctggaa tgtcttatca aaggtggaac cctattctgc taaaggatat
    12601 ctggagccaa aacctgcgaa agaagagaaa gttctgacgc aatcccaagc tctggacctt
    12661 ttaagaaaac gcggatatac cattatagat agtgcttaaa ccaaagttac gttgattctt
    12721 cctaaataga ctcgttgtct tccatttcaa aaattttgaa aacggtaggc ttatttagtg
    12781 taccgttctt caggtatctt caaatagatt tattttcaaa cttatttcct ttcttctttt
    12841 actctcctat agcttagcat ttaagtgggc actcgtgtca ccttgctaga tagctatgaa
    12901 tgccgtaata agcctcctag tatataggca gactgccaat tataactgat tcaatccaca
    12961 ttatgataaa atccaccttt tcgtttcata tgtgttgaaa tatcacatat gtcaaaaggg
    13021 cacgagtctt ttcattcagt gctttgtaaa tactcaaaat tattcctttt cactttaaaa
    13081 aaaagataat tagtacaaaa tcagaaatga ggaaagtatt aggatggact agaaaggagt
    13141 tgttgaattt gttgcaacag attggtcatt tatcaattaa aaattaggtg aagttttatt
    13201 catcactgca gctgctgttt cccagagaat aaaacgtcta gaagaaactg gtcagttact
    13261 gtattatcaa gcagcaattg actacgaaca agcttgatta ctaaccaaag cgtttatcca
    13321 cctgttgatt aaatcaaatc aaaaatcagg tttttatacg ttcataaaag caatctccag
    13381 tgtactcgaa tgtgactgta tcataggtcc ttattcgatg ttaatcaaaa tggggtttcc
    13441 ttctaccgaa tcattggaca gttttatcaa tgatttatca aagtttggac gaaccaacac
    13501 ccaaatcgtt ttctcgactc ttgtttcctt aatggtttat cagttctgga atgtttatca
    13561 tgcgctttta ttaaaataga aaagagcttt tttcatgcga caaagcagtg atcatttatt
    13621 ggcacatgca atctttgtgg atccattttt tgaaaatacg atatttagtt ttgacccaaa
    13681 aatctttaaa tacgtatttg a
//
LOCUS       DACDIP010000064        13679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDIP010000064 DACDIP010000000
VERSION     DACDIP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.064"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IV257_002194"
     CDS             complement(<1..902)
                     /locus_tag="IV257_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8815291.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IV257_002195"
     CDS             1185..2153
                     /locus_tag="IV257_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8815292.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IV257_002196"
     CDS             2266..2544
                     /locus_tag="IV257_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8815293.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IV257_002197"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IV257_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8815294.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IV257_002198"
     CDS             3010..3756
                     /locus_tag="IV257_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8815295.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IV257_002199"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IV257_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8815296.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IV257_002200"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IV257_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8815297.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IV257_002201"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IV257_002201"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8815298.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IV257_002202"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IV257_002202"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8815299.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IV257_002203"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IV257_002203"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8815300.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IV257_002204"
     CDS             8493..9035
                     /locus_tag="IV257_002204"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8815301.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IV257_002205"
     CDS             9058..10614
                     /locus_tag="IV257_002205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8815302.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IV257_002206"
     CDS             10628..11530
                     /locus_tag="IV257_002206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8815303.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IV257_002207"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IV257_002207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8815304.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IV257_002208"
     CDS             12973..13395
                     /locus_tag="IV257_002208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8815305.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4521 a         2448 c         2980 g         3730 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca
     9901 ggtcgtgaag cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgcctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaagta attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgctgctc agccgtgctg tgaagaaaac tggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattacaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggggaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca
    13561 gaggttgtaa aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttattt
//
LOCUS       DACDIP010000065        13619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDIP010000065 DACDIP010000000
VERSION     DACDIP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.065"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..621)
                     /locus_tag="IV257_002209"
     CDS             complement(<1..621)
                     /locus_tag="IV257_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8815306.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDL"
     gene            complement(869..1705)
                     /locus_tag="IV257_002210"
     CDS             complement(869..1705)
                     /locus_tag="IV257_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8815307.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1716..2645)
                     /locus_tag="IV257_002211"
     CDS             complement(1716..2645)
                     /locus_tag="IV257_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8815308.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2837..3190
                     /locus_tag="IV257_002212"
     CDS             2837..3190
                     /locus_tag="IV257_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8815309.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3264..3920)
                     /locus_tag="IV257_002213"
     CDS             complement(3264..3920)
                     /locus_tag="IV257_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8815310.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4146..6080
                     /locus_tag="IV257_002214"
     CDS             4146..6080
                     /locus_tag="IV257_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8815311.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6166..7389)
                     /locus_tag="IV257_002215"
     CDS             complement(6166..7389)
                     /locus_tag="IV257_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8815312.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7383..9281)
                     /locus_tag="IV257_002216"
     CDS             complement(7383..9281)
                     /locus_tag="IV257_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8815313.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9275..10393)
                     /locus_tag="IV257_002217"
     CDS             complement(9275..10393)
                     /locus_tag="IV257_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8815314.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10476..11309)
                     /locus_tag="IV257_002218"
     CDS             complement(10476..11309)
                     /locus_tag="IV257_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8815315.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11302..12219)
                     /locus_tag="IV257_002219"
     CDS             complement(11302..12219)
                     /locus_tag="IV257_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8815316.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12446..13570)
                     /locus_tag="IV257_002220"
     CDS             complement(12446..13570)
                     /locus_tag="IV257_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8815317.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4249 a         2722 c         2235 g         4413 t
ORIGIN      
        1 taaatcttta cataaatact catattcgag tgatttttta tattttttaa ttgtgccgtt
       61 tacgagaatc tcttcatctt tcaatactag gcctatttta tttttataac ctggcatgct
      121 cattagataa aggatttcat cgtaatggat atagtgatcg ctattaccat aacggacagt
      181 tgtccacctt tttttcgtag catgttgaac aggatagttt tctttttctt tcaataagta
      241 actttggatg ctttttttat ttttttcata agtgaattct tttatcaaat aaccactagg
      301 cgtaatcttt cgattaataa cagttattgc tttattcgga tgagacgtta gataaacaat
      361 tttacacttt tcattctttt ctctaagcat ttcgcctagt tcgataccgc tataataggc
      421 gttcaaatcg atgtctagga aaaaaatatc tgaggaatag atttcattat ttttaatgaa
      481 ttcaacaaat tgtataacgt tcaatccctg aacgatctca aatttccaag gacttaataa
      541 aattctggat tcttccaaaa tttctactag cattttttca aaaaccaaac tatcatccaa
      601 aataaatata cgcgttacca taaaaagtac ctccactcga tattttacta tcaaaatcat
      661 ttcatacaac ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt
      721 ccaacacctt tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca
      781 aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt
      841 ctgagctatg tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg
      901 gagcagagac tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt
      961 gataatagtc tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta
     1021 ctccttccgg catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa
     1081 tgccgaatcg gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc
     1141 cttcatagct gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa
     1201 cttctaaata gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca
     1261 actcattcat ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta
     1321 agtattgtgt ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc
     1381 ctttggcttc tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta
     1441 ctggcaaagc tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt
     1501 catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct
     1561 gaactaaagc caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga
     1621 cattgatata ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc
     1681 cagataaaat caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata
     1741 acgctaaggg caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc
     1801 atcatcagta gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt
     1861 gctgtgccga acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga
     1921 gacatcgtaa aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc
     1981 gttagcataa ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg
     2041 atcaaataac gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt
     2101 tgatggatca gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga
     2161 tgatggtgat tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga
     2221 aaataggcaa gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag
     2281 cttgccatct gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg
     2341 atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca
     2401 aattgatgaa cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc
     2461 gaaccagtaa aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg
     2521 gtcaaaaata cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct
     2581 tcaataacga tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa
     2641 aacatgagtt tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt
     2701 caaaaaaaac aagttgctct gtaaattttt acacaactct tattttttac caaagcactt
     2761 caattgactt tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat
     2821 aaaagggaga ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca
     2881 acagtcatcc gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt
     2941 tcgtccacga ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc
     3001 atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat
     3061 ttttcatcaa ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg
     3121 ttatctacta gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg
     3181 aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc
     3241 atttgttcaa tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca
     3301 gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat
     3361 acatctgacg gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct
     3421 tccgttaact gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact
     3481 tcgatttgtt ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg
     3541 tctacgatgc gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta
     3601 ttactttgat ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc
     3661 gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct
     3721 acatcataac catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc
     3781 gttgcactat ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca
     3841 gcattattta aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt
     3901 ggaataatat gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt
     3961 ctccatcata gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt
     4021 ttattcttta ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg
     4081 tagactacta attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg
     4141 attccatgat attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat
     4201 ttgaaaacat acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg
     4261 gcgctggaaa atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa
     4321 agatcgctaa aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt
     4381 ccaatgaaac agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag
     4441 agcggatgcg cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata
     4501 gttcttattt aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg
     4561 gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg
     4621 atcagaaaat cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc
     4681 ttttgcaaca ccctgacatt ttgattctag atgaaccaac gaaccattta gatatcgaga
     4741 cactttcctg gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc
     4801 atgaccgtta ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa
     4861 tgcgccatta caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa
     4921 gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg
     4981 ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag
     5041 aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca
     5101 gtatagaaaa gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg
     5161 ataatgtcac cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc
     5221 tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc
     5281 cttttatccg tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag
     5341 gtcaagctga tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta
     5401 ccactccaga aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg
     5461 tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt
     5521 tagcgatgaa caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg
     5581 acaacaaaga agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt
     5641 cccatgaccg atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg
     5701 gcagcaaagt ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag
     5761 aaatcgccga attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat
     5821 ctgattatta ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta
     5881 ctcaggttga agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa
     5941 tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag
     6001 aagctgctag acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat
     6061 tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt
     6121 tttctttttt tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga
     6181 ccaattacta gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg
     6241 tgtatttacc ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc
     6301 ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg
     6361 gtataagttc ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca
     6421 aaccatgatc accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa
     6481 atcccaataa caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt
     6541 gcttaaatca aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat
     6601 cccccatgct tggcctctag cccaacagga attatcacta taaccttgat gcgtacttcc
     6661 atatcttggt tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc
     6721 tcttactaaa taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg
     6781 atttccagtt tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat
     6841 cattctacct tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt
     6901 ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg
     6961 atttcccgtt attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg
     7021 atgattagtg ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc
     7081 tgtttgttga aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca
     7141 aaatccttct gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc
     7201 gcttgggaat tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg
     7261 acaacgctcg atctcattat tccattcttt ttccattaat tctttataac attcatttat
     7321 ccatttttct ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc
     7381 ctttagcata attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc
     7441 ccatcaaaat caattttggg acgattgttc catacttcta aactataatc ccgatcttga
     7501 tgagcaacca ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca
     7561 ggaataaaag tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg
     7621 gattttcgtt taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc
     7681 accctatagt tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca
     7741 atagcaaacc ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga
     7801 atctgccagc cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc
     7861 catgtagagg caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg
     7921 aactgtccat aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct
     7981 cgctgaatcc ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt
     8041 atcgaaaaac caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta
     8101 tggaaatttg gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt
     8161 atgagcaaat tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct
     8221 ttagccgtcc aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc
     8281 cataatggag ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa
     8341 ccaatattca ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc
     8401 cgattgacta accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata
     8461 gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt
     8521 gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta
     8581 tatatctggc taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga
     8641 aatgcaaaag gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt
     8701 tgaagataac aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga
     8761 taaccaaatt tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct
     8821 gcacattcat attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta
     8881 tctaattttt tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt
     8941 tctaccatac gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct
     9001 gttccagctt ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt
     9061 gcgggccccg ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca
     9121 tatcctaccc cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg
     9181 agtggttgat ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt
     9241 actggatgtt tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt
     9301 ctaatcgaag taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag
     9361 tcagttctct atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc
     9421 aatttgcata gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt
     9481 ttgatcgtac agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc
     9541 cagcactggt atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag
     9601 ttttttcgat tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg
     9661 gattttttgt gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc
     9721 tccaacaaga attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt
     9781 ctgcatcaaa ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg
     9841 ttgtatctgc atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag
     9901 cccaatacag taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt
     9961 cattccatgc tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg
    10021 catgtaaccc tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg
    10081 atggtaagaa taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt
    10141 ctaataattg ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa
    10201 agagctgcca taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct
    10261 ttattacgtc tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt
    10321 ctaattccgc atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg
    10381 tctgttcatt catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct
    10441 agaacgaact agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca
    10501 ttctccttaa atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt
    10561 gctaaaatta gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata
    10621 tttttgataa ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat
    10681 agatattgat tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat
    10741 aaaatcggta cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca
    10801 gcctccatca gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt
    10861 gtactggcaa tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc
    10921 ataatgtaaa ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact
    10981 gtattgaaca acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt
    11041 gtagaactgg ctgtagctaa aactgtactg attacagcaa taacaatcga attccaaata
    11101 atcaagggat aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca
    11161 aacttgaaat tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc
    11221 atatataaat atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa
    11281 attctgcttt gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc
    11341 cgataatata acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc
    11401 aaccatttca aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg
    11461 tccattggaa gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt
    11521 ggctaaaact gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa
    11581 ttgccgaaat ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc
    11641 ttgaagacca gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat
    11701 gatcattgat attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag
    11761 cgcattcaaa aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc
    11821 tacagccacc gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc
    11881 ttttaactgt gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg
    11941 aattgatcct gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg
    12001 gaagatagct agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa
    12061 actaaaagca aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa
    12121 aggtgtgacc aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct
    12181 gttctttatt gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt
    12241 tttaatcacc agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat
    12301 atgaattttc aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg
    12361 tagcatctga ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca
    12421 atagttcata gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt
    12481 gataggccaa tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt
    12541 catctgtcag cataaatgta agaaattgtt gtgccacttg tggatttttg gcatctttag
    12601 ggacataaaa acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag
    12661 gaatcagtgg agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg
    12721 tagcatacca gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt
    12781 gtgcaccgta gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat
    12841 gtttcgcttc tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct
    12901 gctgggctag catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa
    12961 aactaccagt tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga
    13021 tttcttcaaa atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg
    13081 gggtaaaatc actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat
    13141 tcaatttttg atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac
    13201 tataaaaagc atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga
    13261 taatatccgg catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg
    13321 ttcccgaagc tacattcaca atttttaagt caatatttgc tccagttttg cggttataga
    13381 tttttgcagc attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg
    13441 tagtggcttt ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc
    13501 cacatgcact taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc
    13561 tttttttcat gtctatccct tctttctttt caagttctta tataaaacgc tttcactta
//
LOCUS       DACDIP010000066        13353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDIP010000066 DACDIP010000000
VERSION     DACDIP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.066"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            431..484
                     /locus_tag="IV257_002221"
                     /pseudo
     CDS             431..484
                     /locus_tag="IV257_002221"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            488..1087
                     /locus_tag="IV257_002222"
     CDS             488..1087
                     /locus_tag="IV257_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8815318.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            1103..1579
                     /locus_tag="IV257_002223"
     CDS             1103..1579
                     /locus_tag="IV257_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815319.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMHE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            1596..2498
                     /locus_tag="IV257_002224"
     CDS             1596..2498
                     /locus_tag="IV257_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815320.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            2571..2762
                     /locus_tag="IV257_002225"
     CDS             2571..2762
                     /locus_tag="IV257_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815321.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTKRRAFTNRSIR"
     gene            2927..3514
                     /locus_tag="IV257_002226"
     CDS             2927..3514
                     /locus_tag="IV257_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815322.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            3514..4413
                     /locus_tag="IV257_002227"
     CDS             3514..4413
                     /locus_tag="IV257_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP8815323.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            4413..5327
                     /locus_tag="IV257_002228"
     CDS             4413..5327
                     /locus_tag="IV257_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815324.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            5379..5594
                     /locus_tag="IV257_002229"
     CDS             5379..5594
                     /locus_tag="IV257_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815325.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKKFWLNNFSYKDFIKEKANTLY"
     gene            5783..6037
                     /locus_tag="IV257_002230"
     CDS             5783..6037
                     /locus_tag="IV257_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAP8815326.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            6216..7259
                     /locus_tag="IV257_002231"
     CDS             6216..7259
                     /locus_tag="IV257_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP8815327.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            7432..7656
                     /locus_tag="IV257_002232"
     CDS             7432..7656
                     /locus_tag="IV257_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815328.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            7659..7916
                     /locus_tag="IV257_002233"
     CDS             7659..7916
                     /locus_tag="IV257_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815329.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            8018..8371
                     /locus_tag="IV257_002234"
     CDS             8018..8371
                     /locus_tag="IV257_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815330.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            8424..9218
                     /locus_tag="IV257_002235"
     CDS             8424..9218
                     /locus_tag="IV257_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815331.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            9247..10365
                     /locus_tag="IV257_002236"
     CDS             9247..10365
                     /locus_tag="IV257_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815332.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            10398..10718
                     /locus_tag="IV257_002237"
     CDS             10398..10718
                     /locus_tag="IV257_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815333.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            10973..11866
                     /locus_tag="IV257_002238"
     CDS             10973..11866
                     /locus_tag="IV257_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815334.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            11886..>13353
                     /locus_tag="IV257_002239"
     CDS             11886..>13353
                     /locus_tag="IV257_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815335.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKEFEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLN"
BASE COUNT         5131 a         1678 c         2763 g         3781 t
ORIGIN      
        1 gataataacc tcaaaaaata taatgctctt gtccaacgct atatcgaaaa caaaataacg
       61 atgcaagagg tcgctgatga attaggcaaa tcaaaaagcc tagtatcaca cgactttaag
      121 cacaatgttt ttccgaaccc gattgagctg aggaaaaaat ttcaaaagag agtttatcaa
      181 gaaataggga agtggattga tgaagggaat gcaattgacc ctttaattga aaaatatcct
      241 tttttaaaaa gcaaaaataa aattcaaagg ttaattcgtc aaggcgatat tcaagctaaa
      301 gaacctgttt tgacaaccaa tgatttggct aaaataaatc ttcataaaca aattattgaa
      361 gactttgaaa aaaatctttc aacaactgaa attttaaaaa aatacaatat tccgagagga
      421 aagctaacgt cgactctttt tagagaatat ggagaatcgg cagttactaa agcaaaggaa
      481 gtagcgaatg agagaaaaaa acaaaaatag tagtcgatac ttagtagcga ctgtagtttt
      541 tgtttgtgta ttgattttcc tgtttgattt tttacagatt ggaaaaagta taattggaaa
      601 tacattgagt gcaaaggaaa tggttgcttt aggtggtttg tatgaaggac atttgttccc
      661 gaatatattc atttctatcg tttctcatat taacttgtac catttaattt ctaatttatt
      721 gttcttagta gttcttgaag aagcactgaa tgatcagtat gagtggttaa cattattgat
      781 cgttttaatc atatcaggat ttctaggaag cttagttcct tttctgctac aaagcaacat
      841 cgtaagtatg gggcttagtg gaggtatttt tggatggatt gctttatttg cgattgcata
      901 catccatttt gatagcaagg aacatttatt actaagtgtt tttgtattat tgataatcgg
      961 cattttattg gtatctactt ttgaatcagg cgaggcaaat gtcttgtcac atgtaatcgg
     1021 atttgtctgt ggaatgatct tgggatttat tctaaagaac gtttcattga tcaaacaaaa
     1081 gaattaaaag ggagtgctaa aaatgccaaa aagaaaagaa aagaaaattc cagtgttttt
     1141 gtctgtctca gaaatgttat cacttcaaat actgttaaga aaaaagattg aaacaataga
     1201 cgaagaagtt agaaataaaa gggctatgca cgaacaacgt gaagcgctaa gagaacgaat
     1261 ccactatttt gatgatatac acactaaaat ctatgaagca cgtacaaatc acgaagacga
     1321 aagtaaatta ggattagctc aacaacggat acttgaatgg cttaatacga atactgatca
     1381 agaaccgtta agagtgatta gtaatctttt tgaatttgga acgaagcatt ggtttaatgc
     1441 tgtagagaag gatgctgact tacaagaagc gtttgatacg ctaagtaagg atagcaaatc
     1501 catgcgagat ttattgatta cttatctaaa tgagcatgag tttgaacaaa cgaaaaccaa
     1561 aaagaaaaat aaagaataga aagttgtgat gaatcatgct attaaccctg ttaggttatt
     1621 tatgtttaat tcttgctttt tttggggcaa tcgctataat tattggtcaa tttcaaacaa
     1681 ataatccgat tatgcgtaac ataggcattg gaattgtgat tgtaaccttt attgtgttta
     1741 atgccactaa gatttttgga agtaacgata aacaatttgg tatatcggga gttaataata
     1801 gttatgctcc agattctttg ggaagggtag taatagatgg aacatttgaa aatgtaacga
     1861 gttataaaaa tgtttcgctg acagtgaagg aagatggcaa gaaagcatca gacgacgttc
     1921 tcgctactat taagccgtct caatcacaat ttgaagtgtt ttatacagtt aaagagaata
     1981 gccctgtaca caagctgaca ttggttttta acgttgatgg agaagaatac tataaagatg
     2041 taaaaataaa taaaacatca acgaaaaaga ttatcgaatc agcgaaaaaa gaagtagcta
     2101 cagaagaaag tacaactgaa tcaagttcta ctgtcgaagc ggaactacca gataaaattg
     2161 attttgatcc tgaaaaagtc aaatcgtttg aatctgtttc tggttctatg gctgatcggt
     2221 ttaacactga agtcttcaaa gctatcaaaa ctgttaaatt ggatactgaa aagcaaatga
     2281 ttaatgttac cttattcaat tcagttgaat ctatggatca ggataaaaaa gttacggttg
     2341 caagagaact aggaaaaaga gtaattggtt tgtatcgtgg tactgctaaa ccagattcag
     2401 acggtgcaga tttgccagtt catgtagttt atgaagacgg taaagttttt ggagattcta
     2461 cagcaaacga agcattaacg attaaattgg ctaaatagcc agaaagttat aataaacaaa
     2521 tggtgtcaca ccaataatat ataggtgtga caccttggag atgatagctg atggtagtaa
     2581 ataaagaaaa agttagaaag tatgtgactt taaaaatagc ttctgttaaa tatttagaaa
     2641 agcgagctaa agaagaagaa agagcaagag gtaagaaagt aacagtttca gagttgttag
     2701 aagaattgat cgagaatgat caaacaaaaa gacgtgcatt tactaatagg tctatcagat
     2761 aacaatataa ttaataaaaa aagaaagagt gatttggtct tagactaaat cactcttttt
     2821 atataaagtt tctttacact cattccgaat aatgttaaaa ttctttatac gatatatagt
     2881 tccttgtcag aggaagagat aaccagcaat attggaggga taataagtgg aaaacaataa
     2941 tgatattaga cattttagca ttggggaaag agttacaaca gtaagtgaat tatcaaagga
     3001 ttatagagat aaagagggaa cgattatttc tcttaataag aatcgaagag gcgtttggca
     3061 atatgttgtt gatttaggtg atggcgttac agttgagtgt aaagatagtc aactgatcag
     3121 aaataatggc agtcattcca ttgaaaaagc aattcttgta tacccaaaag tatcaagtaa
     3181 gatcatgaat gactggttgc ttggaaaatt tggtgagtac aataaaaaag aaatcgcaga
     3241 agtgatcggc aaatcttatc caacagtatt agggtatcta aaaaagataa cgagtaaaga
     3301 agcaaaaatt gaggatgttt taacagtaag agagcttcaa tcattaatcg agtatcggaa
     3361 taaacaaata acaaatcctg agtaccttag aaaagaagat ggtctattga aacaattgga
     3421 acaagtaaat aaaatccgga agatgcaaaa ggtagctcct ttaagccttg atacctttaa
     3481 aaaaatctat ttgaaagaga tcaaaaaaga ctaatgtgga ctgtaacttt accgttccaa
     3541 cctgtatctg caccaagacc aagtgcaaga aggaatgctg ctgatgaaac taatgacgta
     3601 gcagagaaaa ggatttcaac ctactatgat aaaaagtatc ttgattattt gaaaaaaata
     3661 aagagcttta tcgaagatca agatttatta gatgatgaat tttactcgat tgtaaatgat
     3721 cctatgggcg caattatgga agtggacttt tactaccaga taccaaaaag ctataaaaaa
     3781 gtctataaca ttatgaagac aacagcgcca gatttagata atttagtaaa aagtgctatg
     3841 gatggtattt ttaacatctc agctatacga gatagccata tcacaggctt aattgctttt
     3901 aaatataatg tggcgaacga aggaaaaaca gttgtgagga tcaaacgata ttcagaattt
     3961 gagtgggata cttctgaagg attgaacgga gatatatggg aagctacatt tgattttaaa
     4021 ccagtagcaa cgccaagacc aaagtctaaa tttagaggaa atgggattat cacttactat
     4081 ccaaaagagt acaacgacta tttggaaaat atcaagaata cgcttaaaga gaaagatttg
     4141 gtaaatgatc aactgaaaaa ggtaatgaat gctcctatgg gggttatagc tcagatagat
     4201 tatttttacc aagtgagaaa agataagaaa aagcttgatt cactgatgcg tacttcacag
     4261 ccggatattg ataatttagt caaaggaaca ttggacggaa tttttaacaa ggaaataggc
     4321 atcaaagata gcagggttgt agggttaatt gcttttaaat acaatacttt tgaaaagtcg
     4381 aaaacaaacg tgagactaca ggagatgggc tgatgaataa taatgaaaaa attcttcatg
     4441 tacttgatag ctttgaaata attcaagaag aactaaagaa atatcgggat gttttggaac
     4501 aaagatatga ttttgtcaat cagcaaaaga gtaatcacat ggatttcatt ctcaatatga
     4561 atgacttgaa gaagaaatta gttgaacgaa aagaacaaga aaagttgatc aaagcttact
     4621 ttgaactcgg tgaaaaagaa gttaaaaatg caatggaact taatgaagag cgcagggttt
     4681 tagatcagct gttagaacaa ttattggtga tgtttcaaaa aggacgaata gatgaggatt
     4741 taattgaaga aggattgaga aaatatcctt caaactcagg tatcggaata gtgctgaaag
     4801 cgattgatga agaagaaatt gaagctttca taccgccaga ggattttgaa tcggcaatgg
     4861 aatatataaa atattatagc caagggatca ctgcttttag agagtttgat cctgaagatg
     4921 ttattcatga cttgaacaac ctaaaagaat ggtgtgaaag ttatggagtc gatgattccg
     4981 gattggacta cttgatcagt attatggaaa tcgaagaaga gatgccggat aagcctgatc
     5041 caacagatat tttagaattg atccatgaag ctagaaatcc aattgcctat atttctagag
     5101 gatatactgt attggaatac tataagcctt atatatcagc gatgaaccat ttaagacgtg
     5161 tattaaggga aaaacgtgaa taccgctcag tgttgaatgc aacgaataga ttagaaaaag
     5221 ctgtttcaga gctagatgct tattatcgtg aacattacct gcaagcaggt ggtatgccaa
     5281 gaaatacgaa agcaaatatc agtaggtata taaaaaaagc tgagtaggcg gagttagccc
     5341 ataaaatggc aaaaggtaac ctgaaagggg cattttgaat gaaaagggat gtttttgaat
     5401 tgtacgtatt agaacgtcta gaagacggca tgatggcaca agagataatt gatgaaattg
     5461 atgatatgat cagcagattt ggtcaattgg agttgaatga tggaaacaaa aattcggtag
     5521 tgctaaaaaa attttggttg aacaattttt cgtataagga ttttatcaaa gaaaaggcaa
     5581 atacattgta ctgactaaat tcacgaggag cagtgatgag agcttattgc taagagcagt
     5641 taggctcttt tttgtttgtg taaaattatt tgttttataa tacacattta tacacaaaaa
     5701 atattgacgg atagtacaca aatgtggtag gatatatttg taagcaagtt agattaacaa
     5761 gttaaaggaa ggaagagcag ttatgaaaaa agaagaacta atagatatat ttcaaattgt
     5821 tgaacgagca aataatatgg gaattatgtt ttttgatcgt atttctttaa agatggattt
     5881 aagcgttgcc caccaagaat ttaatctgcg attaaaagcc ttgttgaatt ctgatgatgt
     5941 taatttcgct catgatgttg taggtataca gaaccatata gatagagaaa acaaacgtat
     6001 gggtgatgaa ttcttaccaa gatattcaag tttatgattt ggcgaaagat tcaagagtat
     6061 gtgtcattat tgataggtac tcttgaattg gaaaggagga atgaatttaa gacaacaacg
     6121 attatgatct taaaacaatc gtttatgtaa ttggcaatct tacgaataat tttcatgcaa
     6181 taatgacaat aaaaaatgaa aaggggtaaa atcaaatgaa aaaattttta gtaggaattc
     6241 tattcacaat ggggctagta ttattatcac cttcagtttc agcagaaagc tctatttcca
     6301 tgtatcgtat gtataatccc aatagcggag agcatctata cactagaagc gttggagaaa
     6361 gagacaatct gaaacgttat ggatggcatt acgaaggtgt cgcatggcaa gctccaacaa
     6421 gtggtactcc tgtttatcgt ttgtataatc ggtataacgg tgaacatttt tacacagttg
     6481 atgccggaga aagagataag atttcacaca caggatggac gtacgaaggt gttgcgttct
     6541 attcctacaa cggagcaaat agagttcctg taacacgttt atataacaaa tacgtgaatt
     6601 ggcatcacta cacgttagat caaaatgaaa agaacgtaat tagtaaacaa ggttggaaga
     6661 ttgaaggtac aggctggtat gcaatgccaa acagtgtacc aaatcctaca cctgctccaa
     6721 gcactgaaaa agtgttgaac gttcctgttg tatggcaagg aaatacaatg ttatgcgaag
     6781 gagcttcttt attagaaggt ttacactaca aaggtgtagc cacaaatcaa agcttaacca
     6841 gttttgtaaa ctatatgcca aaagctaatg acaataatcc ttatcatggt tactcaggag
     6901 agtggcgtca taacgttaat ggaacgtatc aaggcatgat ggctgaccca gtagttaaat
     6961 gggcaaaaaa cgttggcggt aatgccgtca atatcactgg ttcaggaacg aatggaatca
     7021 agaatgaaat tcgcaatggt aatccggttg taacttggat tacttataac tatgctacgc
     7081 cagagttcaa acaaatgcct tggggtcgtg cagtatggaa tggacatgta gttctcgtgg
     7141 atggcttcag aaatggtgca taccatgttg ttgaccctgt atttggcgca aaatggatca
     7201 atgcaagcac gtatgagaga gcatttaatg tgactggaat ggcggttgct gtacgttaat
     7261 tgatagaatt atcaactaaa tgaaataaga gcttaattac tgaaatggta aataggctct
     7321 tattttttta gaggaaattt ttgtaatacg aatctataac aaaaaatatt gaatgtagta
     7381 cacaaaagtg gtatattaaa atcgtagaga ttatgcgaag aggtgatttt gatgaaccaa
     7441 agaagaagaa aaaggttaga agaaataagc gagcgacttt ccgagctaaa ggatgagtta
     7501 gaagtaattc aagaagaaga gtacaaagaa aatataccta aaaatctaca gggatcagaa
     7561 agatatgaaa aagcagaaga atccgttgat caacttgaag aagctgttga tggtttatcc
     7621 gacatattag aattgattga agatatagct agctagagat gattaatgaa gatttcagga
     7681 gtgagaataa aatgattgat atttcactga tagagaagtt acttttctca aataagtaca
     7741 ccgcaaaaga gctggctaca agatggcaaa tatccgaatc agctattaaa gcctatcgtc
     7801 aaaataaggg aagcgtagga tggcgtgatt ggaaagggat cagcattgga aaagcagaag
     7861 acataatgaa acgtgaagga cttcgatcct ataccacttc tgaaaaagac aaataactta
     7921 atgtttgaaa agataaaata tctgtcttaa agctatgtca aattcaaatg gtgaatgttt
     7981 gctaaatata atgcaaaaaa aggaagggaa atattaaatg ataagcgaaa atttgtctta
     8041 tgagaataat aaacatctag ttaaagaaaa taagaaagta gaagattata agaacatgta
     8101 tatagaaatg aaaaaatcac cactgtgtat tcaatataaa aatgataatt ggaagcaagt
     8161 agtcgaattt gtaaaacctc atagtatacg aagagtacga agggggaaga aaaaaagtga
     8221 acttgtaaat agcggaagag cggtaattag agttataacc aaagattatc cagttgaata
     8281 tggagattat attttaaggt taagttatca tgcttttgtt gtgttgaaag aagagcattt
     8341 caacgagttt ttcaatttaa aaagtaagta atcatatttt tttgtagacg tgtataaagt
     8401 taactaaatt gcgaggtgat gacatggtag caaaaaagaa tactctaaaa gaagcttctg
     8461 aaaaatatca agatatgaaa agacgaggat taataccgaa agacaaaaaa ggtatatgga
     8521 gagaatttgt taaggaagtc agtagtgatc gaagtggcgg tgactttgtt ggacgatata
     8581 cagcaaaagg ctatagcaaa cgattaagga tcactgttga atatggaacg aatgaatcag
     8641 ctacagagct gaagcaaagg tacttagata cacgaaggga agcttcaaaa aaatataatg
     8701 atttcaagag cagaggtctg attcctaaaa agaaaaaggg aatttggcgt gagtttttca
     8761 aggaagaaga tagcaatcgg aataagggag acttcattgg acgatacaca gcaaaaggct
     8821 acactaagaa tttagggatc aaggttgaat acggaactgg tgaaagctca tgggaattga
     8881 gaaatcgcta tttaaataca ttgttagaga aatataaagc agaattattg gagttgttcg
     8941 atgttaatga ttttatcgag atggctgaaa agtatggtca tgaagaaggt tttatgatca
     9001 atgacattat ggttattgat aattttacgg atttattagc acttgcgaaa aaagcaaggg
     9061 atttaagaga aacgcagatg tatctattca gaaataaaaa cggaagaaac cggagaactg
     9121 aatcgagtga gattgaagat tactacgatg cctttacaca cgatctggga gcctttgtag
     9181 acgagataag tggtgtaatg aacaaatata gggtctagaa taaaaaagcc ggagatgagc
     9241 agataaatgg gaaacaatag catatacaca cctgaaatgc agaataaaat taaaagactt
     9301 gtcggattag ggtggatgac aagaggaaaa ttagattctt ttagtacgat caaatatctt
     9361 ttgatggaca tttaccgtca gaacaagcaa acaggaatac attttttggg ttatgataag
     9421 ccggaagatg cacctttctt tgttccaagt tcagcctata gagccagtac ctccggtagc
     9481 atgtataact tggatttgtt tgaggaagga gcaaagatga tcgaatatga tttcaacgaa
     9541 gcctattcga ggatcatgag aaattatcct ttgccgtcaa acacttattt gaaagcaaaa
     9601 tataatacag atcaagctat tttgagagcg ataaatagac caaaagtaag accgtatgaa
     9661 aatttaaaaa cgtatatgtt ctttttggtt aattttgaag gaacagccaa agaaggaaca
     9721 tatgctaatc caaaatcaca gtcgatgttt gcggattatg gcgaaaatcc caaaacagct
     9781 aatgagccta tttggttatc ggagattgag ctaacactta tccttgatta ttatgatatt
     9841 aaagagatgg aagtcctaga tagccacaca tttttgacga aaaaaggatt actcaaagaa
     9901 tattttgaaa gaatcgagcc attaaaaact gatcctgata cgttgaagct ttataaaaat
     9961 cttagaacga aagtatttgg tgctatcgga caaaaatcat taagagaagg agaaacaagc
    10021 accttcgata aatacagtat gtataatcga gcgtattcct ccatggtagc aggcgtattt
    10081 agagatcgta tggttagata tgaacagaaa tacgttcata gcgcatatgg gctattaaaa
    10141 attcggactg atggattgta tttcagatcc gaagtaccag agtttgaaga gctgtataaa
    10201 cttggagtag ttaagaaaaa agttcataca atatccaaag aagaagtcga aaaagcttta
    10261 gaatttaaaa acaaaggatt tgaaaataag ggctcctttg ggaactctaa agatgaagtt
    10321 aagggtgttg tgatagacga tgatttcgag tttgattttg actaaatact ctttggaaaa
    10381 gatggtagga ggtaaatatg aagaagaaaa ttgtggaagg aaatgtaggt gttattattc
    10441 taaaagattc aactgactat gctaaatcaa tggcactaaa agacgaaaaa gttgcacgca
    10501 tggataaaga tttagttgaa tttctgatgg atgatacaga aagattaaaa ggggatcagt
    10561 ttttgagttt aatgaaagac aaaggcttaa acacatatgg tttatttgat tataagaaat
    10621 tagctataga gtgggtaaaa gaaggagaaa tattttttat tgaagaatgg gaaggtactg
    10681 agaacttagt tcttcatcag tatcttttga aagcttaatc taagtaaaac agttctgact
    10741 agttatgctt gtcagaattt caaaggatgt tttccataac catcatcatt caaaaataaa
    10801 ggatttgaaa ataagggttc ctttgggaac tctaaagatg aagttaaggg tgttgtgata
    10861 gacgatgatt tcaagtttga ttttgactag atttttttga aaggtttaag agtaaacaag
    10921 gtaagaacta agatcatgaa gattgagagt aaagaagata gaggacgaat gtatgagaac
    10981 acactatatt tatttagaag ataaaattgt agatgaaatt ccggcaaaaa gtcgagaaca
    11041 agctagaaaa ttatttattg aaaaatacaa agagatcaat ggagatagtg aagttccaaa
    11101 agtggtcgta acagttagaa aaaggcaaac aaaaaagaga agtacgtttc atgtcagccc
    11161 tgatatatat aatcttatag agcgacatag aagtgaagat aatatttcta tctctcaata
    11221 tattgctaca gttattgatg aattgatttc aggaaagtat gactatgctt ttgattttga
    11281 ggatgaaaag gattacaaaa gtgttgctgt ctttcaatct gattttgaga agctaaaaaa
    11341 ctatgctggc aggagaaatg ttaattttgc aagactacta gaagagttct actccgaaga
    11401 gttgggagta ggaaatgaat acaagagaga aacttatgcg aaattgtctc ataagttatc
    11461 ccaagcgctt aaagaagtga cagaagttaa agcggaacta gatgctaaac ttgaagaagt
    11521 gaaaaaatat caaacagaga ggaagaacaa catgaagaat caagtggaat ttgacgaaca
    11581 gaaagaattt gaaaaagcaa gaaaacgcta taacaaaatt atgaatgaac ttagctatga
    11641 acacttaaca gttcatgaag aattgagtga gggtacagaa aaatatacga tcgcagatat
    11701 ggttaatgaa gtggaaactc ttttagcttc tttctttgaa aatggtcacg taaacgatga
    11761 atggcgaaaa gaagaaccta aaagggttaa cgcttatatt gctcgattaa aaaggtttat
    11821 tgataaatat aaggagaata gtgacaaatt ttctaaagat aaataaggag agagtgtgat
    11881 tacatttgcc agaaatcgaa ggaatatttt cgccaaaaat aggtaaaaaa cagttagatt
    11941 tcagcagttt tccgagcgta aatagagatt ataatctagg tgatgaggta cagatactaa
    12001 atattgattt caaagaacta gacttcgctg ttgctgtttc caacaaaccc aaggcattgg
    12061 aacgagtgaa aaaaatatac caagagaatg atcaaaatat caaaaatatg atcaaaaagt
    12121 atagacgaga taattcaaaa gttgaagacg ttttattcaa cggtacggca aaacatgatt
    12181 tgaatgttta tatggcatta ggagtggctt tgtactgtaa ttcaattaat cagacagata
    12241 ttttggttaa tatcgtgagg aatagctatc aaaaaatatt aaaaataagt agcgaaacaa
    12301 acccttatta tgcaatgaaa tatcttgggg ataatttatt aaatgataaa aaattgatgc
    12361 acaacactga agagctgtta tctaatttgg ctgcgattaa ctttctattc aaaacaggtt
    12421 ctgaagatta ttttatagat aacgtactaa aaggtgctta cggaacagca ttttatccgc
    12481 tttacaatat gaaacagcat aaagccgaac aaaaggataa caaacttgaa attaaaaaga
    12541 tgaaagaact attaggtata ggatttgtaa agcccaattc aaaagtcact attgcttcgt
    12601 tagcaactac taacaatgat gggaaaatag taaatgggtt tgacgattca gatatagata
    12661 tattgtcaaa atcatatttt tctttgtatg gagattttgc agacttgatg ggtattgata
    12721 gttacttagt caataatttt gagatcaagg gtagcgagct gaatgatatt gtgtatttcg
    12781 ttttaatctc tctatatgca attgatcaga ataccgagta ccgacaaatt gactatttgc
    12841 tagtttttta tagtttgtta tatcagttcg ttttaactcg gttgtttaaa aatgctaagg
    12901 aagaactgat cctgaattct gattatgaaa tagcaaaaca aattgcaagt attagaaaag
    12961 aaaacaaatc tgaagtaatc aaattaagaa aagaattaag tattgcgagt aaagaaaacg
    13021 gtcggttaga atctttagtc aaatcggcaa aaaaagaaga ggaacttttg aagcaagaga
    13081 ttaaagaatt aaaaggaatc agaaaagaat ttgaacagct gaggtttgaa aataaagtat
    13141 taaagcaatt gcaggaacaa gaacatagac aatcattaaa tggtaaaact gaagagcctt
    13201 cactgtctga aatgatagct tatctaaaac ataaaaacat tcttttagtg ggaggacatg
    13261 taaattttca aaataaatta actgaactat taccaaatat taaaatgttt gatgtaagcg
    13321 aaaataatgg aaagatactt aaaaatttga ata
//
LOCUS       DACDIP010000067        13147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDIP010000067 DACDIP010000000
VERSION     DACDIP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.067"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IV257_002240"
     tRNA            complement(77..149)
                     /locus_tag="IV257_002240"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IV257_002241"
     CDS             530..1099
                     /locus_tag="IV257_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8815336.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IV257_002242"
     CDS             1086..1946
                     /locus_tag="IV257_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8815337.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IV257_002243"
     CDS             2136..2840
                     /locus_tag="IV257_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8815338.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IV257_002244"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IV257_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8815339.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IV257_002245"
     CDS             4677..5993
                     /locus_tag="IV257_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815340.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IV257_002246"
     CDS             5994..6863
                     /locus_tag="IV257_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815341.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IV257_002247"
     CDS             6918..7727
                     /locus_tag="IV257_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8815342.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IV257_002248"
     CDS             complement(7830..8459)
                     /locus_tag="IV257_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP8815343.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IV257_002249"
     CDS             8629..9921
                     /locus_tag="IV257_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8815344.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IV257_002250"
     CDS             complement(9997..10659)
                     /locus_tag="IV257_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8815345.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IV257_002251"
     CDS             10884..11168
                     /locus_tag="IV257_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8815346.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IV257_002252"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IV257_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8815347.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattca
//
LOCUS       DACDIP010000068        13075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDIP010000068 DACDIP010000000
VERSION     DACDIP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.068"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(79..2199)
                     /locus_tag="IV257_002253"
     CDS             complement(79..2199)
                     /locus_tag="IV257_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8815348.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="IV257_002254"
     CDS             2429..3607
                     /locus_tag="IV257_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8815349.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="IV257_002255"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="IV257_002255"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8815350.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="IV257_002256"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="IV257_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8815351.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="IV257_002257"
     CDS             complement(4960..6213)
                     /locus_tag="IV257_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8815352.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="IV257_002258"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="IV257_002258"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8815353.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="IV257_002259"
     CDS             7790..9136
                     /locus_tag="IV257_002259"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8815354.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="IV257_002260"
     CDS             complement(9444..9830)
                     /locus_tag="IV257_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8815355.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10709..10921
                     /locus_tag="IV257_002261"
     CDS             10709..10921
                     /locus_tag="IV257_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815356.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="IV257_002262"
     CDS             complement(11199..12998)
                     /locus_tag="IV257_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8815357.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4287 a         2351 c         2576 g         3861 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttc
//
LOCUS       DACDIP010000069        12870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDIP010000069 DACDIP010000000
VERSION     DACDIP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.069"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IV257_002263"
     CDS             complement(451..1761)
                     /locus_tag="IV257_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8815358.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IV257_002264"
     CDS             complement(1773..3191)
                     /locus_tag="IV257_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8815359.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3395..4963)
                     /locus_tag="IV257_002265"
     CDS             complement(3395..4963)
                     /locus_tag="IV257_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8815360.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4967..5377)
                     /locus_tag="IV257_002266"
     CDS             complement(4967..5377)
                     /locus_tag="IV257_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8815361.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5374..6297)
                     /locus_tag="IV257_002267"
     CDS             complement(5374..6297)
                     /locus_tag="IV257_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8815362.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6294..7172)
                     /locus_tag="IV257_002268"
     CDS             complement(6294..7172)
                     /locus_tag="IV257_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8815363.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7159..8496)
                     /locus_tag="IV257_002269"
     CDS             complement(7159..8496)
                     /locus_tag="IV257_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8815364.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8499..9602)
                     /locus_tag="IV257_002270"
     CDS             complement(8499..9602)
                     /locus_tag="IV257_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8815365.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9676..11184)
                     /locus_tag="IV257_002271"
     CDS             complement(9676..11184)
                     /locus_tag="IV257_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8815366.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11150..11842)
                     /locus_tag="IV257_002272"
     CDS             complement(11150..11842)
                     /locus_tag="IV257_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8815367.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11864..12838)
                     /locus_tag="IV257_002273"
     CDS             complement(11864..12838)
                     /locus_tag="IV257_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8815368.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
BASE COUNT         3808 a         2766 c         2090 g         4206 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactat taaattacag atgatctttt ccatcatact
     3421 tgaaaaactc aaaatcagca taacttcctc tttttttatt tagatcatgc actgctatcc
     3481 cgataaaatt tccagtaaac cctcctgaga gtatacacat gtttttttgc ggacctactt
     3541 tcttccattc ttcagttgat acgtctttca aatgaaactg tgctgtctct ttctttacag
     3601 tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct atttgtgcta
     3661 ctccgttttc tgattctatc atacgtaggc attttccctt gtcctcattc caagtaacat
     3721 aacaataaat ataattggat tcatttaagt acagtaccag tccagccatc tgattaaaat
     3781 tattaggcat gtattcaaat gctgtctccg catcaaaagt aaaatccgtt tgccgaatag
     3841 ctaacaaatg atgttcgaag gtagattgaa gagattcacc ggaaatcaga cgtaaaaaac
     3901 ctggtctaca ttttgtatca caccaatcag catttggcaa aatgcgtctg gcattccagt
     3961 tcttctttag ctctccttca aatgtatcca taaactgatg atctttttct tgttgtacct
     4021 cttcatttgt ctcaatcatt atttcttcca tcggagttgt ctggctatct ttaagtctta
     4081 accaatcgtc ggacgtccaa tacacttctt gaatagcggt ttctcttccc aaaatagccg
     4141 caccgtttaa tggtctggta caaagatatg tcatgtacca tcttcctgac ggggtttgta
     4201 ctaagctccc atgtcctgta cattgcaatg tagaagcagg tttgtcacta gctgtcagca
     4261 taggaaaacc aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg caaactgtaa
     4321 ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa ataataatac ccgttacgaa
     4381 aatacaaatg tggtgcctcc gttttcgcta attctgtacc tgcaaatatt ttatagatgg
     4441 gaccatcaag cttcttcttc tctgcatcat attcttgtaa tacgataccg ctggatttat
     4501 tcggtgtgtc cagacgataa tcccatagtt cattgacaag ccattttttt cccgtttcaa
     4561 gatcatggaa caaagaagga tcaaagccgt tactatttaa ataaattggt tcggaccaag
     4621 gacctgtgat ctcagatgca gtgatcaaat aattgtggac atctttaaac ggacggcctg
     4681 cacttttgac atctgtgtaa acaagataaa acaatccatc agcataactg atctgtggcg
     4741 cccatatact tccatttgta ggatttcctt gcaaagatac ttgagtggtc aaaatatccg
     4801 ttgtatgtga ccagttgact aaatttttcg actcatatac tcggattcct ggaagccatt
     4861 caaaagagga gacaataatg taataccgct ctttcactct taatatatta ggatctggat
     4921 tgaatcctct taaaatagga tttttcactt tgatagtctg catgattcag gctccttttc
     4981 tcattcttac tcgatataaa ctgaatgccc cagctgcgaa ataaaaaaat gcaaaaggaa
     5041 acgcaataaa ctgagtgcca atcaacaata tgaatcctgt tataaaatac aaaccagtgg
     5101 cagttgttaa gtattttcct ttagcagtca aaaacaatgc tactgctaaa aaagcaagtg
     5161 aaataaacaa cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt
     5221 ctaaagacaa agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg
     5281 cagacatatt acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac
     5341 cgaccattcc tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa
     5401 atgtatccat tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt
     5461 gacagtctgt tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc
     5521 acctatctta cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata
     5581 gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt
     5641 ttgatttttc ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg
     5701 gatatgacac tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc
     5761 aatccagcaa tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca
     5821 aaaaggagta ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata
     5881 accagatagt cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc
     5941 ctgtcctacc ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac
     6001 attcgatcct tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag
     6061 atactcctgt tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt
     6121 aaaattggat aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac
     6181 ctttggatgg ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa
     6241 atccgtcggg gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa
     6301 tgctccctta tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct
     6361 gttaaagaaa aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc
     6421 ccttttctca gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag
     6481 ctaagacgag ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat
     6541 agatttggca catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtatt
     6601 tcgatgtcca cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc
     6661 acccaccagc ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct
     6721 gtgtcttccc ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc
     6781 cataacgttt ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg
     6841 cgttcttcaa atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact
     6901 tctgcaaaag atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact
     6961 gaaaaatctc cgccgtgaga atgcaggtaa aggaccggcg gaaacggtcc ttctttttct
     7021 ggataagcat agagggctgg tactgtttcc aattcattta agtacaattg atagtattca
     7081 agcataaaac cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta
     7141 gatggagcac ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc
     7201 atccatattt tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc
     7261 taatagaaat gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc
     7321 cgcaaagcca atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg
     7381 aacaccggga actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg
     7441 tatgaattgt tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt
     7501 gatattcgaa aaatggatcc ttctgaatgc tggtgtccgt tcatctatcg gataagcctt
     7561 tttttcccat acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat
     7621 aaatggacac atcacattat ccatgactat attggatact cgaatatctt caacgatccc
     7681 gcctcgacct cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt
     7741 tgaaatggta atattacgaa tactaccact catttcactt cctaatacga caccgccatg
     7801 tccatggacc atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt
     7861 atcttcagtc cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact
     7921 aatacgcaca tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg
     7981 gttcaagatc gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca
     8041 acttggtgac tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact
     8101 catcaattta ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca
     8161 tttttttcca tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg
     8221 agcatagata caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac
     8281 aggataatct tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc
     8341 gacattgctt tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt
     8401 cccgcctccg tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc
     8461 atcaattgat gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc
     8521 aatgcttgca agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg
     8581 tagtaagcat atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt
     8641 ttattcaagt tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga
     8701 tgtgatttct tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct
     8761 ttagccattg cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt
     8821 tttgtttctg ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat
     8881 gcagccagtg tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt
     8941 aattcatagg tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga
     9001 tgggatagac ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga
     9061 atcagcaaat ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa
     9121 acatcttggt aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa
     9181 cccatatata agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct
     9241 ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta
     9301 tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg
     9361 tcgatattgt attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag
     9421 tcgatattgt cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat
     9481 tggaaccctt taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct
     9541 tcatataagc gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc
     9601 attatttgct ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg
     9661 tcccaatcgt ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat
     9721 tcgatcgcct ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag
     9781 ctgtcccatg atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac
     9841 ttcattgttc gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt
     9901 ttctttgatc aattgattag agaattcttt ttcagggtct gtatctttga aagagaacgt
     9961 cacttcacta ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc
    10021 gataaattcg atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt
    10081 catcaattta tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa
    10141 ttgtgcttcg tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc
    10201 ttctgaccaa acatgataac cgccgccttc accatctgag atcttgttta caggcactaa
    10261 ttccatatca tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc
    10321 agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac
    10381 tgcaaagtct tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt
    10441 catatactct tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc
    10501 atttggtgct ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg
    10561 cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt
    10621 aaattgttta gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt
    10681 atccaaccag tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac
    10741 gccgtacaag gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa
    10801 atttttatag tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt
    10861 gcggacagaa gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc
    10921 agaagccaat gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt
    10981 cagttccgta tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc
    11041 atgatgcaaa atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact
    11101 gttacttgaa ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa
    11161 ttttacgagc gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt
    11221 tttcttctgt tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg
    11281 ctgtccacgt gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca
    11341 ttgtttggat gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat
    11401 gaagatgtga gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat
    11461 cataatgagc catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa
    11521 tatacaaagc gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa
    11581 tcccgatggc gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat
    11641 atgtgaagta cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca
    11701 ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta
    11761 aaccgcccca cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta
    11821 ttttatctaa tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt
    11881 ctcctagctt tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt
    11941 taaacaatcc cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa
    12001 tataggtgtc gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat
    12061 gctcgaaccc taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg
    12121 gacgaattgc cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt
    12181 ctgcagcttc atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag
    12241 accagcccaa ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg
    12301 atgttagaaa actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc
    12361 cgtcaacatt caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag
    12421 ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata
    12481 gtaaagacaa aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca
    12541 acgtatttct taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga
    12601 aaccgaccca aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa
    12661 cgagtccccc cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca
    12721 tcacatataa aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct
    12781 tttcttcttt tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa
    12841 gcccctcctt agttttgtga atgtttgctg
//
LOCUS       DACDIP010000070        12795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDIP010000070 DACDIP010000000
VERSION     DACDIP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.070"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            183..1055
                     /locus_tag="IV257_002274"
     CDS             183..1055
                     /locus_tag="IV257_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8815369.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(1513..3711)
                     /locus_tag="IV257_002275"
     CDS             complement(1513..3711)
                     /locus_tag="IV257_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8815370.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            3943..4935
                     /locus_tag="IV257_002276"
     CDS             3943..4935
                     /locus_tag="IV257_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8815371.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            4995..6416
                     /locus_tag="IV257_002277"
     CDS             4995..6416
                     /locus_tag="IV257_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8815372.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            6409..7908
                     /locus_tag="IV257_002278"
     CDS             6409..7908
                     /locus_tag="IV257_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8815373.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            7947..9341
                     /gene="uxaC"
                     /locus_tag="IV257_002279"
     CDS             7947..9341
                     /gene="uxaC"
                     /locus_tag="IV257_002279"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8815374.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            9500..10183
                     /locus_tag="IV257_002280"
     CDS             9500..10183
                     /locus_tag="IV257_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8815375.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            10185..11021
                     /locus_tag="IV257_002281"
     CDS             10185..11021
                     /locus_tag="IV257_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8815376.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            11032..12786
                     /locus_tag="IV257_002282"
     CDS             11032..12786
                     /locus_tag="IV257_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815377.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         3996 a         2457 c         2410 g         3932 t
ORIGIN      
        1 ttgtgctata caaaacgata aagtgtattt tttcacaaat aaactacgca tagcaaacct
       61 tctatttttt tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg
      121 atgataaatt gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct
      181 atatgaaaaa gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc
      241 taattttgct agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg
      301 cttctacaca agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag
      361 acgcgtatcg ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg
      421 gtgttacagg tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc
      481 tttccagaaa gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt
      541 tgttcagcgg ggggatgatt ccaacgttct tagttgttcg ttctctaggg ctggtcaatt
      601 cttactgggc catgatcctg cctgtggctg ttaatgcaat gaatatgatc attatgagaa
      661 actttttcca agccttgccg gatagtttgg aagaatcagc aaaaatggat ggttgcacag
      721 attttggtgt atttttcaaa attatgctgc cattagcgtt accgtctatt gctactatct
      781 cgctttttta tgctgttact tactggaaca cttatatgac tgctattttg tatatcaacg
      841 attcatctaa atggccgata cagattcttt tacgccaaat cgtcattgta tcaagtggga
      901 tgcaagcaga aagtagtgcc gttgatgtga ttccaccagc acaaacagtt aaaatggctg
      961 ttatcgttat tgcaacagtg cctatgctga ttgtttatcc ttttgttcag aaatattttg
     1021 tcaaaggagc actcgtaggt tctgtcaaag ggtgattatg tactgaacgc aaaatggtag
     1081 ttgtgtcaga agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat tgcttcttaa
     1141 atttttgtga aatttcggct tatttttcga agaatctact cctggaacac cgtgtatcag
     1201 aggttgtgaa agagacgttc agccttgagc gatgataaaa actctcacaa acattgcgac
     1261 gagtaaccca caggagcaat aagaagcgat tttgaaaaat agcttcccat atttcacaaa
     1321 attatgcctt attgtcgata gcctgtctct tgaacgccat ttattcgtaa aaaagaactg
     1381 tgacaagact tttgtcacag ttctttttgc gtgcttagcc ttgaaattta actatccata
     1441 aatacaaata ctagggttat tctaacttct tttattgctt gaatcagtcc acaagaaaaa
     1501 cagcctcttg attcacaaat atttttttcg atattctccc ggcgtaatcg attccttctt
     1561 tttaaagaaa cgaataaaat tttgcggatt tcgatattgc aatctttctg caatctcttt
     1621 tacggagagg ttagtctctt tcaaccattt ttttgctacc tctaaacgat gattttgaac
     1681 aaaatcacca aatttttctc ctgtttcctt tttaaataca ttacttaaat agttagggtt
     1741 ataatgcaac ctatcagcga tcacatctaa agacagatca cgatcatatt ctgtgtagac
     1801 gatatgcata atattctccg acaaattttt aaattcatga tcagtgattg cttgagagca
     1861 ttctgtgatt ggcagaacta attgttctaa aacaagtttt tctagttctt tgggatgata
     1921 attgttcagt gcttttacat aaatttgttt catgttatca aagttttttg aatctactcc
     1981 caataactgt cctaattgaa cgagttcatt gatcaagcgg accgaaacta cttcccgact
     2041 caaaggattt ttattttgtg caaacaatgc atcaatcaat tcatgtacta aattcttcac
     2101 ttcctgattt ccagaacgaa tggcttcaaa caatcgattt tgttcttcta tcggatatct
     2161 aataagtgtt ttttcatgag cagcaggaac gatttcctga taaaaaataa ttgaacttgg
     2221 accagtattt acacgataat acagggcttc ttttgcccga tcaaccgatt gcttgctttc
     2281 attcagcgtt tcgaaccggt tactgatgcc tatactgata gttaaattca agtattcttt
     2341 tacacgtttt tgaatgagtc ggcaatactc catgactttt ttgcttgctt ctgcatcatc
     2401 tttatccatt cgataaatag tggcttgcat ttcttcattc aaaacgatcg gcagcattcg
     2461 attttccttt ggcacaattt ccgtgatcat gttgttgatc gctaaaagta gcaaatcacg
     2521 ctccccaccg tgactatctt ctaacagatc gatttgaacc aaagctgtat aatagcagtc
     2581 attttgagaa ctataaccaa attgctgcaa ccgctgatct agttctcgcc tttctactcg
     2641 atttctaaat aaactaagta caaatagcgt ttccagctgt ggcttttggg taactaagtg
     2701 tgcacttaac gcttcttttt ctcctatgat ccgactgact gattgagcga ccaaatctag
     2761 ttctttccca ctgaatcctt ttgatttaag attcagacgt tcctgtattt gagaaatcgg
     2821 acgggcaaat ctgtccgaaa ataagtagga aagaagacca atgagtccta ccagtgttaa
     2881 agacactgca actagcccga ttctcagatt gcggatcatt gcgccaattg cactagagtc
     2941 aacttctaat ctgtaaatcc aattattata atctgatttc agtactatat acgttttatc
     3001 tttttctctt atcactttca gctgattgta atctagatcc acgtccgtta atacagttgg
     3061 ttgctcttta ttttttactg tctgagtagt aaaaaggttg ccaacttcgt tttcaataga
     3121 aagtatgtct tctccacttt caccaacaac ttgttctatt gtatgtttct ctatcgtagc
     3181 aattcccaat gcatattttt ctttttcaaa cattggcaaa gaaatcacca tttcgattcc
     3241 tttgtttttc ttttgccaaa ataaattatt tttgttattt atgtacttct gttgatactt
     3301 ttcaacttcc tcttcggtca gttgactcaa cgatccattg attactcccc atttaccagc
     3361 caaactgact aaatcataat tattatcatc cataacaatc gtttctataa aatttagttc
     3421 tttcctgatt tcagaataag tggagtaatc tgtatatgtc agcggatgct tgaatatgtc
     3481 cgagaaattt ttggtcgaac catacgtaga aaacgaatat tcaatcgttc taattttcca
     3541 ttcaacgtta gttttaactt gcctgagcaa attctttttg tctgttaaat aactcgattc
     3601 gatcgattca taggtattct ggtaaatgaa taaactaaat cctatgacaa gtacggctcc
     3661 taataaaaac atcggtaaaa atattttata aaatactgta cttttcttca ttttcttcct
     3721 ctcctccttt taaaataaaa ggctttcatt tttctgtaaa attgatactc atctaattaa
     3781 atgagcattc cccgctttca agaatagcat aattaaaaat aaaaatccaa gtaattttta
     3841 acgtgtgcaa tcttttcata tcaaagatct attgacttat cgtagttatc gtaatatgat
     3901 gaatgcgtta acaaaaagtg aaaaagtaag gaaaatgatg gaatggtaac gatcaaagat
     3961 atcgctaaag cagctggcgt ttctcatacg acagtttctc gtgctttgaa taacagcagt
     4021 ttaataaaaa atgaaacaaa acaaaaaata cgacaactag ctaaggaaat gaattattcg
     4081 ccaaactata gtgcaaaagg tttagtgaat caaaaaaaat atctaatcgg actatttttc
     4141 tcaagtttgc ataaaggaac ttcatcaaat ttcctagcag attccattgc aggcatccat
     4201 tcagtattag acacaaatta cagtttatct gttgaaagta tcgatgttgt tgatttaaca
     4261 gaaatcaacc tgcaacgcta cgatggactg atcgttatga gtcaaagtga tgatgatcag
     4321 cctttcatcg actatctcaa agaaaaagaa tttcctttcg ttgttgtcaa tcgtcacatc
     4381 aatgatccag cggttatcaa cgtcactgcc gatgatgcgg ccggtgttat gcaggcagta
     4441 gattacggta ttggatgcgg acatacgaag atcgcttata ttggcggaaa gaaaaatttt
     4501 cgttcgacaa aagaacggga aaaaggggta ttgaaaagtc taaaaaaagc gagcatccct
     4561 gtcaatccag cctatttttt aacaggagat tacagcttag aaagcggcct ccaaaacatg
     4621 gaatacctat tatcactttc tgacctgcct actctggttt tttgcggcaa cgacgacata
     4681 gcgattggtg cattgagagc tgtacattta gctggcctcc gtgttcccca gaacatttct
     4741 ctgatcgggt tcgatgattc tccagtcgtc tcctatctca atccgccgct taccaccgta
     4801 cgtaagcctt tgaaagaaat gtgtcaagaa ggaacaaagc ttttactaga tttgatcgaa
     4861 ggcaggacga tcgaacaatc taaaattgag ctctccacct tgttgatcct tcgtgagtcg
     4921 atcaattact tgtaaactac aagtaatttt atataaaaat gttaacgtgt gcataacaca
     4981 gaaaggaagt ttatatgaaa aaactgacaa aagaattagt gaaaaaaaac gaagcacctg
     5041 taaaagttct tcagtttggt gaaggcaatt ttatgcgtgg atttattgat tggcagattc
     5101 agcagctgaa taaccaacaa ttgtttaaag gtaatgttgc aatcattcaa cctttagaac
     5161 aaggtcttgg aaatatgatg aaccagcaag ataatttata tacggtcatt ttacaaggac
     5221 tcagcaatgg cgaaatcgtt gatacttccg agattatcac cgtagtagaa caaatgatca
     5281 atccttatga tgaatgggat gcttatctat ctctagcaga aaatgacgac ttggcctata
     5341 tcgtctccaa tacaacagag gcaggaatcc aattcgtgga gactgataat ttaacagata
     5401 ctcccccaca gagcttcccg ggcaaactga cagccttttt ataccgacgt ttcaaattaa
     5461 acaaagctgg ctttaccatc attccttgcg aactgattga cagaaacggc gaacgtctaa
     5521 aagaaatcat tttacgctac gcagaaaaat gggatttaga acctgctttt gttgagtggt
     5581 tagaaaaaga aaatgttttc tgttgtagtt tagttgatcg catcgtccca ggttatcctc
     5641 gcgaagatgc aggtgttttc aatcaacggc ataactatga agatcaattg atggtcaaag
     5701 cagaaccatt catgctttgg gtgatcgaag ggcctaaaga attggagaat caactaccat
     5761 tcaaacaggc tggcttgaat gtcatcatca cagatgatat gactccttat cgtgaacgta
     5821 aggtgcattt gcttaatgga ccgcatacag cgatggtccc tcttgctttg ttggcggaat
     5881 tggaaactgt agaagaagta atgaaagatc ctttattctc cgcatatgtt gatcagttat
     5941 tcaatctaga attgattcct atgctttctt tacctaaaga tgaattagcc atttatgctg
     6001 accaaatcaa agaacgtttc ttgaatcctt ttgcccatca caaattagaa gcgatttcat
     6061 taaatagtgt ttcaaagttc agcacccgtc tgcttccagt gttcaagaag tatatcgaag
     6121 aacaaaatca ggtaccaccg ttaatcactg tttcactagc tgcgctactt cttatgtatc
     6181 gaggcgatca agtaaagcca catgatgatg aaaagacgat aagcgaattt actgatgctt
     6241 ggtctgataa tggaacagct attcctcgat tattgcaaaa cgctgcgctt tggggagaag
     6301 atttgtctca aataccgaat gtgacagata cagttcaaga aatagctgca aagatcgatc
     6361 aaaaaggtgt ccggtcagta ttacaagaaa tgaaaggaac gatggtccat gcttgaaacc
     6421 ctgcaaccaa caatgaaatt aaatcgacga gacagtgtgg tcgtcgcatt aacccctatt
     6481 ccaaaacaaa cacaactgac gattgatcag caaacgatca ccactcttga agatatcccg
     6541 caaggtcata agattgcatt gggtgatttg aaagctggag acaacgtgat caaatatggc
     6601 tatccaatcg gtcatgtgac gacagacgta actgccggtc aatggctgca cactcataat
     6661 gtaaaaacca atctttctgg tgaattggat tatcgatatg aaaaagatgt ccatccaagt
     6721 cattaccttt ttgagaaccg aacatttcaa ggttacttgc gaaaaaatgg aaaagtaggg
     6781 attcgtaatg atttgttttt agtacctacc gttgggtgtg tgaatggcat tgctgaactg
     6841 atcgtcaaac agttcaagga aaaacatcca gatctgggac agtttgacca tattacgatt
     6901 ttaaagcatc cttatggctg ttcgcagctt ggaaaagatc atcaaaatac tcgagaaatt
     6961 ttagcggacg cagtaaatca tcccaatgcc ggaggagttt tagtatttgg attaggttgt
     7021 gagaacaata cagttcctga attcaaaaaa attttaggtg aatatgatga agaacgagtg
     7081 aaatttctag tggctcaaga tgtttacgat gagatcgaac aaggtgtggc tctattagaa
     7141 gagcttttaa cagcggcgga aaatgaccag cgcatatcag tcccgctctc taagttgaat
     7201 gtcggtttga aatgcggcgg ttctgatggt ctatcgggaa taactgctaa tcctttactt
     7261 ggtgcatttt ctgactactt gattgctcaa ggaggcagca cgatcttaac agaagtgcca
     7321 gaaatgtttg gcgcagaaca agtattgatg gcacgagcgg aaaatgaaga agtttttgac
     7381 tcaatcgttg acttgatcaa tgacttcaaa cagtatttct tgtcttacgg ggaaccagtt
     7441 tacgaaaacc catctcctgg taataaagca ggtgggatca ctacattaga agataagtct
     7501 ttaggctgca cgcaaaaatc aggaagctct cctgtcgttg atgtgctaca atatggcgaa
     7561 aaaatcagaa aaccagggct tagtttattg caagctcccg ggaatgatct tgtagctgcc
     7621 tctgctcttg cttcatccga ttgccagttg gtcttgttta ccacaggaag agggactccg
     7681 tttggctcat atgtcccaac ggtcaaggta tctacgaata caactctttt tgaacgcaaa
     7741 ggtcattgga tggattttaa tgcaggcgtc ttattagaac aaccaatgga agtaatctta
     7801 gaatcgtttg ttcaaaaaat catcgctgta gctagtggag aagaaacgaa aaatgaacaa
     7861 aatgacgttc gagaaattgc catttttaaa aacggagtaa cactttgatt ttcataaaac
     7921 agtataaaaa gaaagaagga agatttatgt ttttatctga tgatttttta ttaaaagacg
     7981 attgggcgaa aaagctttac cattcctacg caaaaaacat gcccattatt gattatcact
     8041 gtcacttaga tccaaaagaa atttacgaaa acaacaattt cagcaatctg acagaagctt
     8101 ggttaggagg cgatcattac aaatggcgct tgatgcgagc ttgcggcgtg cctgaagaaa
     8161 aaatcacagg aaatgcttct gattttgaaa aatttttagc ttggtgtcaa accgtaccaa
     8221 aaattatcgg aaatccgctt tacagttggt cacatcttga attgaagcgt ttcttcggta
     8281 ttgacttgtt gataaatgaa gaaaatgcag agaaaatttg gacacaagca aatgaaattt
     8341 tggcagctcc tgattttcgc cgtcgggaaa tcgcaaaaaa atcgaatgta gaagtgattt
     8401 gtacaacaga tgatcccgca gacgacttgc actatcatca gttgcttgct catgaagaac
     8461 cagatttgaa ggttctacct tcattccgtc cagataaagc actcaatatt gaaaaagaag
     8521 gatttaatgc atgggtcaca ctactggaag aagcagctga ccaaacgatc accacctatc
     8581 aagaactgat agaagtctta gggcagcgga tagaatactt ccatcaacat ggatgtcgtg
     8641 tttccgacca cgctttagac atcttacgct ataaagaagc ggatgaaaca gtattagaag
     8701 agatttttca aaaagcacga gctaacaaac tattatcttc agatgaaatc gatgcttatc
     8761 gtacagaaac actgatacat ttgacccact tctatcattc tcataattgg acgatgcagc
     8821 tgcacatcca tgcttatcgt aactgtaata cacaaatgtt ggaaaaatta gggcccgata
     8881 ccggctatga tggaatgaat gatctttctt tgactattcc tctgcaaaaa ttattgaacc
     8941 gtgcagaaga aacggatcag ttgccaaaga cgatccttta ttcattgaat ccgaatgact
     9001 atcctgcgat tttagctttg atgggctgtt tccaaaaaga acagaatgga aaactgcagt
     9061 taggttctgg atggtggtac aacgatactc gagctggtat gcgcgatcaa ttgacacaat
     9121 ttgctgatgg tagtgctttg gggaattttg tcggcatgct gacagattca agaagtttcc
     9181 tgtcctacac tagacatgaa tatttccgtc gtgttctttg tgaattttta ggcgaaatgg
     9241 tcatgcgagg agaagcacct gaagatgaac aacttttagg aagtctagtt caagcgatta
     9301 gttatacaaa tgctaaaaat tactttggct tttttgccta attgattaat agtaacagta
     9361 acgaatcaaa aacaggatag tgaaataacg gaatccgttt atccacgttt tagagaagaa
     9421 gacggaccaa atttgctccg tcttcttctc ttgctgttct gcttcattgc ctataaacct
     9481 ttatgaaaga agagataaca tgcctactat ccatattgcc ggtgattcta ctgcggcaga
     9541 aaaagatgaa aacaagcgtc ccgagacagg ttggggagaa aaaatctctg cttatttttc
     9601 acccgagatc cagatcaata accaagcaaa gaatggacgc agcaccaaat catttatcca
     9661 ggaaggaagg ttagcccgtc tagcttcaca gtttcagccg aatgatttct tatttgtaca
     9721 atttggacat aacgaccaga aaatccaaga agaaagaggt acgcttccct tcggtagcta
     9781 tttaaaaaat ctgcagatat tttttgaatg cgcaaaaaaa gcgaacgtcc aaatggtctt
     9841 actgacatct gtgacgcgac gagattactt agaaaacgga acattgaatc cagatatact
     9901 tggagactat ccaaaagcga tgcgtgcatt tgctgagaaa caccatattc ctcttttaga
     9961 tgtgttctca agatcacaag aattgttcca gaccttctca aaagaagaga caaaaaaatt
    10021 ctacctgcat cttatacctg atacttgtaa aaattaccca gaaggactaa aagacaatac
    10081 ccactttagt ccagaaggcg ctgataaagt tgcccgaata attgttgaat tgataaaaga
    10141 atcccgtctg cctcttgcta attatttaca aaaaggagtt tgagatgttt ccattaaatt
    10201 taggcatccg tgcccacgat ttagatgttc gaaatcgtga agaaatcgtt caaaaagtat
    10261 cacagcttca cttgtcccat atccaatttg caccacaaaa agcatttcct gaacaactta
    10321 aattaactga catgacactt gggagtgctg cctattttgg tgaatacttc caaaaaaaca
    10381 atatcgaact ttccatttta ggatgctata tcaacctatc cagcaaaaat caagaagttc
    10441 gtgcacaagc attggcaact ttcaaacagc aaattgctct gtgctctgcc tatcaagcca
    10501 agatgattgc tacggaaacc ggtagtgtct ctgtcggtta cacaaaagaa aattttactg
    10561 aagaagctta tcagatcgcc agaaattcta tcattgaatt agtcgctcat gcagaaaatt
    10621 ttggcgtaac tgttgctgtt gaagcaggga tcaatcatcc aatctataac tatcagttag
    10681 ctaaacgttt aatcgatgaa gtagcttcac caaatttaaa aatcatttta gattgtgcga
    10741 atttgatgca taaagaaaat catgctgatc aggaaaaagt gatccgaggt gccttggaca
    10801 atttacatgg ttatattaca gcgcttcatc tcaaagacta tatcatggaa aatggaaaga
    10861 tccgcatggt acctgttggc aagggctgtt tagatttccg cccggtactg cattatatca
    10921 aatatgagcg tcctctaatg tacgccactc ttgaagctac tcctgagatt tatgttcctg
    10981 aagcaataga acacttgcag tttatttacc aagattgtta aggagaagat tatggaggaa
    11041 cgaatacaga aaagttatca aaaatatcat gcaaatgctt ttcagacatt gctgcatctt
    11101 tatcagggaa attattggcg ttttgccatg tcaggactct tttttattat caaacatctt
    11161 ccatcttggt taatgccgat cgctattgcc aatgtaatca atgcagcttc atcgaaagat
    11221 tcttctcagt tgcaggccat ttatttcaac gcttttttga tggcttgtct gattggtcaa
    11281 aatatattga cgaattattt tcatgtaaaa taccatagca cttcgatccg tcaagtagaa
    11341 gctggactaa gagcgaccct tatcaagaaa atccaagaac tatctattcc ttatcaaaaa
    11401 gaattgcaat ctggacgaat ccagtcaaag atcatccgtg atgtagaagc tatccaaacg
    11461 ttgtcctctc aagtatttgt cagcggatta aatatcgctg tcaatttatt cgtagcttta
    11521 ggtatcacgc tttataaaag tcctgttgtt ttcatgtttt tcttattgac tgttcctctc
    11581 gccgcattcg ttactctctt tttccgtaaa aggatcagac agtccaactc agacttcagg
    11641 caagaaatgg aagagacttc tgcaaaagtg atcgaagtga tcgaattagc accgattgct
    11701 cgagctcatg ggttagaaaa acacgaaaca actcgcttaa ataagcattt tactcaaatc
    11761 tatgaaaaag gattccactt agatatcctg caatctgttt tcggatcagt cagctgggct
    11821 agttttcaga tatttcagct gatttgtttg attttcactg ggatattagc tttacaaggg
    11881 cgcatccaac ccggcgatgt cgtgatgtat cagacgtatt tcacgacgat cgtcaattcc
    11941 gtttctggaa tgatcacgct gattcctacg atttcaaaag gcatggaatc tatcacttct
    12001 attggcgaac tgttgattgc tggcgatgta gaaacttatc acggtaagaa aaaaatccaa
    12061 catttggatg gaaattttcg ttttgaggat gtttcttttc tttatccgga ctcactcaaa
    12121 ccgattatcg atcatttttc tctagaggtg aaagctggag aaacaattgc ttttgttggg
    12181 ccatctggct ctggaaaatc tacactcctt aacttattga ttggatttat ccagccgact
    12241 tcaggaaaaa tttttctaga tgatcagcct ttggatgaac tggatatgcg aagtgtgcga
    12301 aattatttag ccgttgttcc tcaaaccact ttgttgttct ccgcttcgat caaagagaac
    12361 atcacttatg gcttgaaaaa cgtatcaaaa gaacgtttag atgaagtcat cgaagctgcg
    12421 caactttctt cactgattga ccagcttcct gatggtctgg ataccttagt aggcgaacat
    12481 ggcaacaagc tgtctggtgg ccaaaagcaa cgtatctcca ttgcacgagc gttgatacgt
    12541 cagccaaaaa tcattttgct agatgaagca acctcagctt tagataacca gtcagagaaa
    12601 aaaatccagc aagccctgaa ttacctgaca gaaacaccca ctacttttat tgtggctcat
    12661 cggctttcaa cgatcaaaga ggcagataaa atcgttgtga tcgatgaagg aaaaatcgtt
    12721 gaaataggta cttacgaaaa actgttagct caaaaaggct atttctaccg cttgaaaaat
    12781 gaatgacaaa aaata
//
LOCUS       DACDIP010000071        12589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDIP010000071 DACDIP010000000
VERSION     DACDIP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.071"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..537
                     /locus_tag="IV257_002283"
     CDS             <1..537
                     /locus_tag="IV257_002283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815378.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..2375
                     /locus_tag="IV257_002284"
     CDS             564..2375
                     /locus_tag="IV257_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8815379.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2436..3905
                     /locus_tag="IV257_002285"
     CDS             2436..3905
                     /locus_tag="IV257_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP8815380.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3916..5925
                     /gene="topB"
                     /locus_tag="IV257_002286"
     CDS             3916..5925
                     /gene="topB"
                     /locus_tag="IV257_002286"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8815381.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6271..6867
                     /locus_tag="IV257_002287"
     CDS             6271..6867
                     /locus_tag="IV257_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8815382.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6880..7779
                     /locus_tag="IV257_002288"
     CDS             6880..7779
                     /locus_tag="IV257_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8815383.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7782..7913
                     /gene="ssb"
                     /locus_tag="IV257_002289"
     CDS             7782..7913
                     /gene="ssb"
                     /locus_tag="IV257_002289"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8815384.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7935..8267
                     /gene="ssb"
                     /locus_tag="IV257_002290"
     CDS             7935..8267
                     /gene="ssb"
                     /locus_tag="IV257_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8815385.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8289..8546
                     /locus_tag="IV257_002291"
     CDS             8289..8546
                     /locus_tag="IV257_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815386.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8705..8827)
                     /locus_tag="IV257_002292"
     CDS             complement(8705..8827)
                     /locus_tag="IV257_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8815387.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9017..9241
                     /locus_tag="IV257_002293"
     CDS             9017..9241
                     /locus_tag="IV257_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815388.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            9689..9895
                     /locus_tag="IV257_002294"
     CDS             9689..9895
                     /locus_tag="IV257_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8815389.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9895..10146
                     /locus_tag="IV257_002295"
     CDS             9895..10146
                     /locus_tag="IV257_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815390.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            10162..10599
                     /locus_tag="IV257_002296"
     CDS             10162..10599
                     /locus_tag="IV257_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815391.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            10592..11287
                     /locus_tag="IV257_002297"
     CDS             10592..11287
                     /locus_tag="IV257_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815392.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            11668..11847
                     /locus_tag="IV257_002298"
                     /pseudo
     CDS             11668..11847
                     /locus_tag="IV257_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11909..12093
                     /locus_tag="IV257_002299"
                     /pseudo
     CDS             11909..12093
                     /locus_tag="IV257_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
     gene            12127..12558
                     /locus_tag="IV257_002300"
     CDS             12127..12558
                     /locus_tag="IV257_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP8815393.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         4647 a         2113 c         2663 g         3166 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aatagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaaacttac tacacgattg
     1441 aaaaagtgtt tgatatcaca caaacacagt taaaacctga agactatccg aaaatctttc
     1501 cgaaccgtgt ctttgatttt caattggata aaaaaggaca gtcagaactt caagctggga
     1561 ttcaagcggt agccaacaat attggaatac aaattcgaga tatgacagaa agtgaagtct
     1621 atcaaagaga attaggacaa gcacgtgggg cgtatgttcg taacgaattt actggcgaag
     1681 aagaaatcgt catgaatact cgaaatactc ctacgcagca tttagctaca agtattcatg
     1741 aattagccca tgcaagaatg cacaaatttt cagaattaga taccgcaact aaagaattgc
     1801 aggcagaaat gacttcttat attgtgtgca aacattttgg aatggacacg ttggagaaag
     1861 ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat aaggaagctg
     1921 cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc attcaaacca
     1981 tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca gcagaagtga
     2041 agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt cctgttggtt
     2101 cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac
     2161 atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt ttaaaaggaa
     2221 aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg ctaggaaatt
     2281 cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt
     2341 atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga ctatctgaaa
     2401 aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa atgagtctta
     2461 ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga atggctgtag
     2521 tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt gatcgaagaa
     2581 aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa ttagctaaac
     2641 tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca gccactgatt
     2701 ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt gagacagaaa
     2761 agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg aattatctga
     2821 aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa gggttgattg
     2881 ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac cgtttagatc
     2941 atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg gcaagttatc
     3001 aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa cacattgaca
     3061 agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag catttaaaat
     3121 tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag aaactacaag
     3181 atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat tacgttgaag
     3241 agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa atatgtgtgg
     3301 acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga atcgttgatc
     3361 cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg caagtaccta
     3421 aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa cctgaagcaa
     3481 ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg gtgtctgctc
     3541 atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta gaaacaattg
     3601 atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa cgcgcagatg
     3661 gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac gttaatgcag
     3721 gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag catgaacatg
     3781 aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag atccagcaac
     3841 aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa caagaaaggg
     3901 agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca agcgaaggct
     3961 tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat ccaagatcga
     4021 ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga gttagattcg
     4081 cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc tattttcccc
     4141 aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt cgtcaaacga
     4201 cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga aggggagctg
     4261 attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt taaaagactt
     4321 tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt acgagacgca
     4381 gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga ttggttaatt
     4441 gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat accaggaagt
     4501 ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt gcaggaaaag
     4561 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
     4621 ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga agcatttgaa
     4681 gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat ccttcaagtc
     4741 gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag ccttcagtct
     4801 aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
     4861 tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac ggaaggggaa
     4921 tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc cgaagcgatt
     4981 ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt ccaggaacat
     5041 tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca actaagtcca
     5101 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
     5161 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt aaaggcaatc
     5221 gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag cgacggaaaa
     5281 gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt gacggtggat
     5341 ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg tacgttgatc
     5401 actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc catattgaaa
     5461 gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac cttgaaacaa
     5521 aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg gattttgctt
     5581 tgccaagccg tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc caaatgggaa
     5641 agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct agccaatatc
     5701 caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc cactgatttt
     5761 ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta
     5821 ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt ggcagcttgt
     5881 acgtctgaag cctgtgattt taaaaggtca gaagggttca tttgaagcaa cgattgtttt
     5941 aaaagaagac ttcactacag gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa
     6001 aagaacacgc cgcactgcaa aataatggag ggttaatctt atgcgtaata ccacacacaa
     6061 acaacaagaa atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa aaatgcgtgc
     6121 cagcaaacga agactgcagc aattgaagaa ccagcagagg attcaaaaag aaatcgaatt
     6181 agatcgataa aatcttccta actattgtat acgtataaac ttatgttata ctgtttttat
     6241 aagtttatac gtatacaaag gagggagaaa atgacaaaag caatggtata caagctgcta
     6301 gaagaattta atggaacttt ggctttgaag gatgccaaaa aagcgggcat ttctcctgta
     6361 acgatcaaac gtatggtaga tcgaggggag ctggatcgag agtatccagg ttttttcacc
     6421 ttacctggtc aatttcctga tgaattgttt atggcgcaaa aaaaatatga acgcggcatt
     6481 atttcacata ttaccgcgtt agatttgtat gatttaacag atatgatccc tagacagatc
     6541 gatctgactg taccttacgg ttatcacgtt tctgaaaaag gattgaagga attcgcagtt
     6601 gagctacact atacaaaagc ggaatggtat gaattaggta agattgaaat aaaaagtcgc
     6661 tatgggaatc ctattatagc ttatgatcca gaaagaactt tatgtgacat ttggaatccc
     6721 tggtataccg ttgaggatga aatcaaagta aaagcaatca aaaactatat ggagtcggac
     6781 agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt taccaacaga caaaacgatg
     6841 cgttcttata ttatggcttt aaattaaagg agaagataca tgacacctac gcagttaaaa
     6901 gcaaaagtga agaatatttc tagagaaaaa gaagttgatc cgcagcttgt tttaagacat
     6961 tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc cttatcgtga aaattttgtg
     7021 ttaaaaggcg gcttcttgat tggctcaaag tatggcattg aaaatcgcac aaccaaagat
     7081 attgatacga ctttacggga aatgaaagta accaaagaaa cattaacaac ggttctaaac
     7141 gaaatttttt caacgccgac gaaagaaggc attcaatttg agattcaagg aatgaaagaa
     7201 acgagagaag ccgattacta tccaggattt agcttacgtg tgctggcaca tttggaaaac
     7261 atgcggccag attttaaggt agatgtgact acaggtgact cgatctatcc tgcaacgatc
     7321 actcacagtc ataaattgat gtttgaggat cgaacgattc ctttggaatc ctatcctaca
     7381 gaacaaatta ttgctgaaaa attaagtgca acctttgatt ttttaacaga taattcgaga
     7441 ggaaaagatt tttatgatct ctatactatt cctaaaatgg agaagatcga tgagaagacc
     7501 ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa aaactgatcc gttaaagata
     7561 tactatgaaa aggatatgga tgtactgaaa aatagtcacg agctaaaaga tacctggaag
     7621 aaatatcaaa aaagcaatcc ttatgctgcg tcgatcacgt acgatgagac aatggattcg
     7681 attagtaatt tgatggaaaa agtgattcac gtcgaagaaa aagagcgaaa actagcttat
     7741 caaagacacc aacagatgaa tagagaaatg gagagataaa gatgattaat aatgtgacat
     7801 tagtaggacg cttaaccaaa gatcctgatt tacgttatac cgcaagtggt actgcagtag
     7861 cgacttttac cctagccgtc aaccgaaatt tcacgaatca aaacggcaat tgagaagccg
     7921 attttattaa ctgcgtgatt tggcgcaaac cagcagaaac aatggcgaca ttagctaaaa
     7981 aaggtagctt gattggtgta gttgggcgga ttcaaacgcg gacttacgac aaccagcaag
     8041 gacaacgtgt atatgtgact gaagttgtag cagataactt tcaactatta gaaagtaaag
     8101 cagcgacaga gagccgtgcg cacgctgatc aatcgtccac cagtccaagc accgccacct
     8161 ttgaacaacg gaatacagcg acgcctaaca acaatggatt gaacgcttca cagaatccat
     8221 ttggtggaca atcaatcgat attagcgatg atgatctacc gttttagttt gagaaagaag
     8281 gagtaagcat ggcaaaaaaa attagtctga cgatggatct attgataccc gtaattgcat
     8341 ttatagacgg gatttctttg ggcgtcactg aaaatccttt ggtaaataga attgcgaaag
     8401 gatcactatt tcttgtattc gtgtatcttg tctgttctta tcttttaaaa agagcaggaa
     8461 aggataattc cttttggcga ttgactaaag tcacagtagc agcgacagct gttatactta
     8521 cggctattct accgctttat atgtagagtt aacgggaaat cgttgaactt cttgcgaaat
     8581 attgctataa tgaacatgaa aaagaggagt tgcgctaaca acccctcatt aacagcaccg
     8641 attaaagaac ggtggcagaa tattagttat ttggaaataa ccgctgacct gctaaagtag
     8701 acggttattt ctttttgtca tttttaagca tatttacaat caaactaatc aaagcaatgg
     8761 taaacatacc aaaaccaaga atggtttgaa ttgtctcata tgcggacaaa aggcttactc
     8821 ctttcataga ttttagcact tacattcata agcaccacca cctttccaga agtcgccacc
     8881 gtcttcaaac tttactgtct tgactattat agcatatcta aaatcgaata aaaattctca
     8941 attttagctg tttttgctat aattttagcg aggtgatcgt gatggaacaa tatattggtt
     9001 tactcgataa attagggtga tcaagacaca gccattgctt gtgcgattta cattacgcac
     9061 gagtcatgaa ccaatcaatt gtgtagtggc caatatagaa atagcggata aattgctgat
     9121 tttggaagaa ggcaagtata ctatttcagt gacaggtcac ttcaataaac gaaatcaact
     9181 agtcattgaa tcaatgcaaa ttagaaaccc tgatcatttt acgagattaa tgggcatata
     9241 aataaaagaa gagctgcagt cctaaatgag gattgcagct ctttttgttt tacatagaaa
     9301 caagaaaaat gaaaaggcct ttttaggaag gcgcaagcgc cttctcaatt gaaaaacctc
     9361 tctctcactc caaccgccac cgccgttcga gaagaaagga agtgaacgtt gcacgttcac
     9421 taagataagg ggtcttgcaa agcaagacaa aatagaaaaa acttcctctc accaaaaggt
     9481 tcgttcctgt tcgctatctc tttattgatt tgccagaccg gcaacaagtt gccttttatc
     9541 accaaaaagc gacaaggaca agggtaaatg gcacgctgca cgcccttgtc cttgtcgctt
     9601 ttagttgatt cattctggcc aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa
     9661 cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc
     9721 cttcagcgaa ttattcccaa actatcatgc accatatact gtcgaatgcg attgtggaga
     9781 aagagcgaaa tgtatcaaaa gctattgttt gtatcaatgt ccaacatgcg ggaaaaaata
     9841 taccttgtct tatggcgatt atattttgat tgatgagaaa ggaaagaaac gctgatgaaa
     9901 acacaacaca aattattgac tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc
     9961 atttctgaaa aagtagcaga agctttactt attccgcggt actggacgat agaaacccct
    10021 ttagtagagg atgcaccgac tatgctattt gaagacctag tgaagattgc aggatatgaa
    10081 ctgcattatc aagaaaacat ggaaacttat gaaaacgaat attggttaac caaattagag
    10141 aactaaaaag gagaaaaaaa tatggctcaa aattactatg acgaatttgt caaattacct
    10201 ttggataaaa tggcacaaaa aatggaggat atgacattcc tatacaatga gacacgtgtt
    10261 ccaaaaaaac attataagga aagattgtcg gtagctgtgg aagaaatgat tgaatcgggt
    10321 gtcgaaatga atctcattgc gacctactat cgaacgcttg aagaactgaa aaagcaaaat
    10381 ggacaatggt ttttccaagc cttactatgc ttggaagcag gcgtcaagcc aaacactatc
    10441 aaaccttctg aataccaagc tttagaacta acgtatggga aatttgtcga aacgaaaaaa
    10501 gccaagactg tttcaagtga atggttggac tacttagaaa atgtcaacaa gtatggcgca
    10561 tgttacatca tgaaaaagga ggacaacgaa aatgaataag gtgatttatc aactaacaac
    10621 ggaagatgtg tcacactttt taacaaacaa agaacatttg aatgaagtaa tcagacggat
    10681 tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag tcgaagaaca
    10741 attgtatttg gaaagtgaaa cgagtcaaac gaaggttaca gtcagcaata attatttaga
    10801 cgtgtataaa cgtcgtcctg acacattaaa aaaaggagcg gagcttctac gactagaaac
    10861 agaaaaatat atcatgaatg tttacctaga aaggagtgaa ctggtcgaaa actttgtcag
    10921 ctacacgatt tgcaggaaac actattactc agaatcagaa tattattcaa tttttgagag
    10981 catggaatta atggaagagc taaacagcaa agatgcgacg gtagacgatt attaccttgt
    11041 atttactaaa tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    11101 actcgctgta cccgaagtat ttttgaggga atatgtagaa gaagtagata acccaggcga
    11161 cgactggtac acgactttaa ctgatttttt agatacctac acttacgacg atacagaaga
    11221 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    11281 tcaataacca tttataccga aaagtgagaa tgtgtgaaat aaaaacaaaa aaccttgaaa
    11341 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    11401 gcgaaataag cgagcaaagc ccgaaaacct aagagaaaga aggagattta tatgaacaaa
    11461 attattccct tattattagt cctatttgtt ggagaaatac ttatgcgact tagtgcagct
    11521 gtacatttaa acctcgcagt atttttggta atactaatgt tttgtttgat cttttggcga
    11581 tttgtattag ccctcgtaag tctactattc aagatgattg cagccatatt tttcatagga
    11641 ttatttcgat gaaaggagag agtaccaatg ccaattgcaa taggaaataa acggttaccc
    11701 gtgacgttgg atgaaaagag acaaaaagaa ttgcagtaac caaagcagaa gtacggcaaa
    11761 agtgaatcca ggattatgtg tattgcgtta gatttattga ttgcgcaaga aaaagcagga
    11821 tttgacatac cagcactcaa aaagtgatcg cactttgcgc acgtgttaat acgtgttata
    11881 attattttgt aaggaggtat agtgcacaat gccacttacg ggaaaagaaa tgcttaagtt
    11941 attgaaaaaa atggctgggt tgagcgaagg caagagggat ttcatcatca tctttataag
    12001 gatggtgtta gaatcactgt tcctgttcat gccaatcaag atcttggccg aggacttgag
    12061 cgaaaaattt taaaggatgc gggattgaaa taatctcgca ttcgattaaa gaaaggattg
    12121 actgaaatgt taagtgagcc tttagcaaaa acctatcctg ctatttttag cccagaagaa
    12181 ggtggtgggt actttattga atttcctgat gttcaaggtg cttacacagg aattaacgaa
    12241 gacgatatat cttatggaat tgctatggca caagaggtgt taggtatggt tttagcagat
    12301 tatattgagc atgaagatct acttccagaa ccaacaccaa tcaataagat ttctgttgaa
    12361 gatgattctt ttactacttt aattcgtgta gatgttgcca aatatttgaa agacacagaa
    12421 cttgtcaaaa aaaccttaac aattcctcaa tgggcggata agctaggcaa aagggctggc
    12481 ataaattttt cagtccttct aacagaatca attgcagata aagcagataa cattcttcgt
    12541 tctggaagaa ataattagaa ctcaaaaggg tcagaaactt aaaaatctt
//
LOCUS       DACDIP010000072        12465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDIP010000072 DACDIP010000000
VERSION     DACDIP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.072"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1034)
                     /locus_tag="IV257_002301"
     CDS             complement(<1..1034)
                     /locus_tag="IV257_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8815394.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
     gene            complement(1063..1380)
                     /locus_tag="IV257_002302"
     CDS             complement(1063..1380)
                     /locus_tag="IV257_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8815395.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(1406..1726)
                     /locus_tag="IV257_002303"
     CDS             complement(1406..1726)
                     /locus_tag="IV257_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8815396.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(2190..3605)
                     /locus_tag="IV257_002304"
     CDS             complement(2190..3605)
                     /locus_tag="IV257_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8815397.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(3622..4938)
                     /locus_tag="IV257_002305"
     CDS             complement(3622..4938)
                     /locus_tag="IV257_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8815398.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(5088..5786)
                     /locus_tag="IV257_002306"
     CDS             complement(5088..5786)
                     /locus_tag="IV257_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8815399.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(6075..7064)
                     /locus_tag="IV257_002307"
     CDS             complement(6075..7064)
                     /locus_tag="IV257_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8815400.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(7144..7977)
                     /gene="msrA"
                     /locus_tag="IV257_002308"
     CDS             complement(7144..7977)
                     /gene="msrA"
                     /locus_tag="IV257_002308"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8815401.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(8152..9786)
                     /locus_tag="IV257_002309"
     CDS             complement(8152..9786)
                     /locus_tag="IV257_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815402.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(9773..11620)
                     /locus_tag="IV257_002310"
     CDS             complement(9773..11620)
                     /locus_tag="IV257_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815403.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKAPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            11607..>12465
                     /locus_tag="IV257_002311"
     CDS             11607..>12465
                     /locus_tag="IV257_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815404.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLI"
BASE COUNT         3870 a         2426 c         1951 g         4218 t
ORIGIN      
        1 actaagacca ttggtgtcaa aataaatgga atgaacatga tcgggttcaa cacgataggt
       61 aacccaaaca tcgttggttc attaatattg aagacacctg gcgcaatcga caagtttgtt
      121 acaaccatat atggtttgtt tttacgtcct actaaataaa tagctaacag taaacctaaa
      181 gttgcacctg ttcctcctaa gttgacaaag ctgtcaaaga aaggtttgtt cacgataaac
      241 ggtgcgctat gtccagctgc gattgcatta acgtttgctt caatggctgg cgcattgatt
      301 gtttgcatca aaggatcgac catattcgct ccgtgtaaac caaagaacca taagaatggt
      361 gtgataaatg caagcagtaa ggcagatgga taagagtttg caagtcccat aaatggttct
      421 tggaccactg tatagaaaga acctacgata tcatcaacac cgaatgcagc aaaaattgca
      481 gcaactaaag caaacgcaga gatagtgatc attgctggta gtaatgaagc aaatgctttt
      541 gcaactgccg gtggcacacc atcaggcatt ttgatgatca atttttcatt tccgcttaaa
      601 cgtacaaaga tttctgttga aataatccca acaataatag caataaacaa accgtttgag
      661 ctcataccta atgctccgcc taaagcgaag aaagagccta aagaaatcac acctgcggaa
      721 acaccgtctt ttcggtagcc gttagctaaa tgataagcaa gcaagaaagc aatcaagaca
      781 gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca tgattcacca
      841 ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt gatcattact
      901 gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg aatcgcaact
      961 aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc cataaatgca
     1021 acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt tcaatcgacg
     1081 atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca tcagatgatc
     1141 ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt cttgtgtcag
     1201 catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt tgattttttc
     1261 atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg catcgctttt
     1321 tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt gatcttccat
     1381 ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct tggtcgagta
     1441 ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa ggaatgcctt
     1501 taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt cctaaaagta
     1561 aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat acagcaaaga
     1621 tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact aggagacttg
     1681 tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta aacacgttcc
     1741 tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag cgctttaaaa
     1801 agttgatatc atcacattta aggtctatac caagttgcat acacactaaa aaagaaagcg
     1861 cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc acaaaaaaag
     1921 ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa gcttgcatct
     1981 gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt gctattctta
     2041 ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca atcagctgac
     2101 cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat aaccagtaaa
     2161 tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg caaatccaat
     2221 tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt tctgcttcgt
     2281 tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt cattggtgac
     2341 acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc ttctgttgct
     2401 ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc ttgcacgatt
     2461 cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc tgttatcagc
     2521 caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat gattttcgga
     2581 tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa gacttcataa
     2641 tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc gcgaacgcgt
     2701 ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat cgtttctaaa
     2761 tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag gaactcttct
     2821 gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa atcatacata
     2881 taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc aggatttaaa
     2941 atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc tttagccgtt
     3001 gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc atgcggccag
     3061 cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa tgtaaaccag
     3121 ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc ataaaaatca
     3181 actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg gacttcataa
     3241 tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat catttcactt
     3301 atatgctgag catattcttc atctactata cagttctcat aatcagtaaa aaaacgagcc
     3361 caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc cacatcctgc
     3421 tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg ccaaacaaaa
     3481 tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt tcctttccaa
     3541 ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg aatagtaatt
     3601 ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg ctacttttac
     3661 aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa taactgcacc
     3721 gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc aagggaccat
     3781 tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg ttccagtgat
     3841 aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg gcataccaaa
     3901 aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg tgatttgttt
     3961 catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg tgactccaga
     4021 cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg gtacttcttt
     4081 tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg gttcccgtat
     4141 cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg caataaacat
     4201 caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt gtacgacatt
     4261 gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc ccgcagccgc
     4321 gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag aaacattcgt
     4381 tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc agcaataaag
     4441 ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat ctgtatattg
     4501 tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt cataaacagt
     4561 tggattaaca gataaataag acatcactgc gatgatacca gggaatacac catccacttt
     4621 attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca agaaacttag
     4681 tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac gcaagaaact
     4741 tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg tgccaaaagc
     4801 acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca tgatatacct
     4861 taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat ccataaaact
     4921 cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt tgtctattag
     4981 tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc aaatataaaa
     5041 caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca attagtttct
     5101 ttttctccta ttattttctg taattctctc cgatttattg ccatatggac agtcattgca
     5161 tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt atgaatatcg
     5221 atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc aattgattca
     5281 ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc aatcatcgta
     5341 tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga agtttcaatc
     5401 ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc cgctgccaaa
     5461 cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat catccgatcc
     5521 tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt atcactgaca
     5581 aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc ccgcacagct
     5641 tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc attcggcagt
     5701 ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc agtagttact
     5761 tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga tgtatttttt
     5821 cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa attgattaat
     5881 ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag gataaaattc
     5941 actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt caataacaga
     6001 cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg ataaaaagtt
     6061 tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc tggtgagaaa
     6121 tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact tgctccgacc
     6181 agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg cgctaatgat
     6241 tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt tgatccaact
     6301 tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc aggaatacca
     6361 tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat tgcccctagc
     6421 ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga aaacatattg
     6481 tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc agtcattttt
     6541 tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc ttgtcttaca
     6601 agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg gcgatgatga
     6661 tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca acttgaaatg
     6721 atggatttac gagagcccca ttccccatat ggcccttccc acatatgata acctgctttg
     6781 ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat acgcccaaag
     6841 tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa atgggtaatt
     6901 cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc ataatcacca
     6961 aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg accggattta
     7021 cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc tcctcctcat
     7081 caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt taaagaaaag
     7141 gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta ctcgttgata
     7201 gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt gttttttgta
     7261 aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga tttttgtgac
     7321 gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt ccgctaattc
     7381 tttttgtcgt tcgacttgat aaaaaatcac tggacgatat tgcttccctc tatcttgaaa
     7441 ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca actcgctata
     7501 acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg tgtaaccacc
     7561 acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg acaaaacaga
     7621 taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc cccctgcaaa
     7681 tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt catatttttg
     7741 ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag gcgtcagatt
     7801 cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt ctttaacgga
     7861 tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc aatctcgtgt
     7921 attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct catccataga
     7981 ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat cataaagtga
     8041 ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga gagaatcctt
     8101 atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa tttacctttt
     8161 atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa gataagaaat
     8221 aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt tgactccgct
     8281 tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat ttatttctac
     8341 attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct cattagttac
     8401 tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt gactgccaat
     8461 ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag gagaataatc
     8521 atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt tgtttacaac
     8581 aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag aaacagttaa
     8641 agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct tattgtatct
     8701 tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt gcaagcttaa
     8761 gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat accatggcac
     8821 caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt tttctatgtt
     8881 tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa ctagtcccat
     8941 tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca cattagatgc
     9001 atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca tttgttcaaa
     9061 catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac taaataattt
     9121 tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac gaataaaaat
     9181 aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt acactagcaa
     9241 actcatactc aatgctagct ttacagacgg atacttttct tcttttccag agatgatttg
     9301 ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg caattgcgga
     9361 acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac taaataaaaa
     9421 tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga gtgttgtaca
     9481 caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg tcatccaata
     9541 taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac catatccagt
     9601 ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc ccttgattcg
     9661 agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt tgaaaaagat
     9721 gaaagaatat atactactaa ctataattaa tccactcaat attactactt ttttatttat
     9781 tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc aacttattag
     9841 gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata ctgatccata
     9901 aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt tctccagcat
     9961 ttacatccac attcactaag ccatgttttt tataagagtc atttacgact ttaccgttta
    10021 cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc tttttgttgc
    10081 ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt tcccacgatt
    10141 ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct tctgttgggg
    10201 cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt tcttcattga
    10261 taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt gctgctatag
    10321 acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat tttttattca
    10381 tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt tggataccta
    10441 agatacgcca aggaaactgg agtaagctta tcggtgcttt ttgaaacaaa aaccatggaa
    10501 ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt ttttcttttt
    10561 tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct aataacccca
    10621 tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa atagaaagtg
    10681 cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc atctgctgca
    10741 acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc tttactaaat
    10801 tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa cgaactataa
    10861 tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta tacacaagta
    10921 gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc aatccataag
    10981 cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt gcggatcgat
    11041 aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat aaaaaagcag
    11101 catctatctt tttaaaaaac ttttctccat aataaaaact aatcagaatc gtacaaatat
    11161 ttaataacca aatatataga acataactta atattaagtt atttgaaatt ccataaaaaa
    11221 cgactgcagg aattattgta agaaaaggat aaaaatagtt cagtccatct ccataattgt
    11281 taaatgttgt atagttgatt ggcccttcaa aaatagaaga taaccctttg attcgtgaaa
    11341 gatgaaaaga taaatcacct gttgtataaa tttcttgagc atttctcata aaatttactg
    11401 aatataaagt actaagtaag ataagaacta ctaaaagaaa tgctttttta cttattttca
    11461 tagatttgtt gttctccttt tttaattcta ttttcagtat actagaattg tcttttttta
    11521 ataagttttg cgttaaattt ttcatagcac tagtatgata tactagaaaa gaatcaattt
    11581 ttattacttt taaataggag aaaaaaatga aaaaaaacaa aaaccaaata atagatatca
    11641 tttttatgtt atttctaagt tttgcttacg tcatccctct attcatgtct aagggaatct
    11701 atcatagtgt caatcaggat acttactttc atttatctcg aatcatagga ctagataatg
    11761 tatggagttc tccagtaaac ttcaataact tcgatcatca tggaacaatg atgaatattt
    11821 tttatccatg gcttactctt tatccagctt ttttatttta taaaatgatg ggaaatcttg
    11881 ttttaagtta taatatctac tattttttta tcactttttt aacaatggtc gtttcttatt
    11941 tttctatgaa acaaataaaa aacaatcgtt atatttcgct gttattttcg attatttata
    12001 cattttccgc ctatcgtgcc attgacatat tccatcgtgc ttcattggga gaagctgtcg
    12061 ctttaacatt tttgcctttg atactaatgg ggtgttacga aatttatatt agagattacc
    12121 aaaaatggta ctggttgtct attggcatga cgctagtagt gtatactcat ttactttctg
    12181 tcgctatggt ttctgtattt attggaggaa cgttgtttct ttctttttat ttttgggacc
    12241 aaaaaatcgc tagactctta agtcttttaa aagctaccgt cctcaccttt tttttaagtg
    12301 cgggattctt aattcctttt attcaacaat ctagagcaca agaattgaaa gtcccactgg
    12361 gaaaagaatt aagtggtatg gctcctagtg atatgctgac acatatttta aataataact
    12421 ataacaacta tactattgga ttattcttat ttctcggatt gattg
//
LOCUS       DACDIP010000073        11736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDIP010000073 DACDIP010000000
VERSION     DACDIP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.073"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(283..2358)
                     /locus_tag="IV257_002312"
     CDS             complement(283..2358)
                     /locus_tag="IV257_002312"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8815405.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2360..3277)
                     /gene="glyQ"
                     /locus_tag="IV257_002313"
     CDS             complement(2360..3277)
                     /gene="glyQ"
                     /locus_tag="IV257_002313"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8815406.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3661..4473)
                     /gene="recO"
                     /locus_tag="IV257_002314"
     CDS             complement(3661..4473)
                     /gene="recO"
                     /locus_tag="IV257_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8815407.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4616..5515)
                     /locus_tag="IV257_002315"
     CDS             complement(4616..5515)
                     /locus_tag="IV257_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8815408.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5530..5931)
                     /locus_tag="IV257_002316"
     CDS             complement(5530..5931)
                     /locus_tag="IV257_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8815409.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5909..6385)
                     /gene="ybeY"
                     /locus_tag="IV257_002317"
     CDS             complement(5909..6385)
                     /gene="ybeY"
                     /locus_tag="IV257_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8815410.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6402..8594)
                     /locus_tag="IV257_002318"
     CDS             complement(6402..8594)
                     /locus_tag="IV257_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8815411.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8618..9589)
                     /locus_tag="IV257_002319"
     CDS             complement(8618..9589)
                     /locus_tag="IV257_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8815412.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10295..11674
                     /gene="brnQ"
                     /locus_tag="IV257_002320"
     CDS             10295..11674
                     /gene="brnQ"
                     /locus_tag="IV257_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8815413.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3204 a         2452 c         2140 g         3940 t
ORIGIN      
        1 catagaaata tttataaaca ggttttccct atataatcat accacaaaca gttttcttga
       61 ctagggaaat tcctaattta aaagtaaata aaacagtaac aagattatta ttaatctttt
      121 tcatttttat ttcctcactt ttcatgagcg ttttgaaaat tctagcaaag aataaaataa
      181 caaaaattat taaaatagaa ttaataacag cttttatatt tataacaaaa aaacagagac
      241 acagcacgtt tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg tgatgatttg
      301 atccaagctt gctaatgaca aagccatggc agttatttga ctaagttgtg ttagtcggtt
      361 cttacgtact gctggttcat cagccatgac catcgtttga tcaaaataat tttcgatcag
      421 tggacgaaga gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag caaaacttgc
      481 tttcattgct tctactgctt tgtgtaattc tttctctgcg tcgttttcga acagctcagg
      541 gtctacttct gattgttttt caagctgcgc ttttttcgct aagttcacga cacgtgtcaa
      601 tgcttccatt gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca attgtgctgc
      661 tgcaaatact tttgtcagat ctttttgttc tgcattcaaa acagcttcga tgacatcatg
      721 acggatattc ttcgttgtca ataattggcg cagacggcct ttgataaagt cgatcacctg
      781 ttgttgtccc gctgacaagc caatgccaaa acgatccgta tctttactga taacttcact
      841 aatcgtttct tgcagaacag acaaaggaaa ggcccatttt ttcgattcga tgattcgaac
      901 gacaccatat gcttgacggc gcaaagcata tggatcgttt gagccacttg ggatcatccc
      961 aactgcaaag aaagaaaaga tactatctaa tttatcagca atcgctaaaa cagaacctac
     1021 tgtagtctca ggtaaatctc cttcactgga tataggcatg taatgttcac ggatcgctgt
     1081 tgctacggct ggtttctcgc ctttaagcag agcatatttt tctcccatga tcccttgtag
     1141 ttcagggaat tctcctacca tgttggttac tagatcaaat ttatagattt cagcagcgcg
     1201 ttgcagatcc atcaattctt cctcagatag tcctacttgt tcgcctatga tgcgagcgat
     1261 gacttgtacc cgttgcattt tctcatagat gctaccgatt ttttcatgga aagtaacagt
     1321 ttttaatttt tcaacgtatt ggtcgattga agctttcttg tcttcattat aaaagaattc
     1381 cgcatcttct aaacgagcag tcaatacttt ttgattgcct cgtaccacat tttccaattt
     1441 tacccgatcg ccattacgag cagaaataaa atgaggaagc aacatacctt gatcgttccg
     1501 tacttcgaaa taacgttgat gttctttcat agaagtgact agtacttctt caggaacaga
     1561 tagatatttt tcttcgaagt cgccagaaaa gacagttgga tattcgacta gattgttgac
     1621 ttcttctagt aaatcttcat ctagatcaat gatccagtga tttttttctg ctaattcgtt
     1681 tgcttgtgtg atgatcattt cttttctttt ttgcggtgaa gcaataacaa attgttcatt
     1741 caatttttct tcgtattctt ttgcatgcgt aaatgtcaca tcttcgccta agaaacgatg
     1801 acctcttgaa actctgtctg ttttgacatc caagatctca aacgggatta tttcgtcatc
     1861 taacaatgcc acgatccagt gaatagggcg aatatattca aaatcgtatt ctgcccagtg
     1921 catcgttact gggaaagtga ggcttttaat gacatctgct aaacctgtca atacttcttt
     1981 tgcttctttg ccgtgagtgt atttcgttac atagacatat tccacgcctt ttaattctct
     2041 aaaagtgata gcatctgtcg tcagaccttg tccgcggaca aatccttcag ctgctttcgt
     2101 ccagtttcca tctgcgtcca aagcaatttt tttggctggt cctttgactt cttcttcgct
     2161 gtctgcttga cgttctggaa tgtttgttac tttgattgct aagcggcgcg gtgtagaaaa
     2221 cgtttcgatc gtatcataag tcaattggtt ttcatctaaa aattttgctg ttttttcttc
     2281 taattgtttc atgcttggtg tgactacatg agcaggcatt tcttccagcc cgatttcaag
     2341 taataagtct tttgccatct tattcagcct ccttcacgtt ttctggattt gctgtgtttt
     2401 tattcaataa agggaatcct aatttttcgc gttctgccac aaagattttg gctacagcac
     2461 gagccatgtt gcggatacgg gaaagataac ctgcacgctc tgttacggat actgcaccgc
     2521 gagcatctaa taaattgaat gtatggctac atttcaaaat ataatcatac gctggatgaa
     2581 caagattttc atcgatacag cgtttagctt ctttttcaaa acgatcaaaa ttgcttagta
     2641 gcaggtcttg gtcgcttact tcaaaagaat atttagaatg ttcatattct ggttgtttga
     2701 agatctcacc atatttcacg ccatcagccc attccagatc ataaacactt tcaacttctt
     2761 ggatataaga agcaagacgt tctagtccat aagtgatctc agaagtaact ggtttacaag
     2821 gtagtcctcc gacttgctgg aagtacgtga attgtgtgat ctccatccca tcaagccata
     2881 cttcccagcc cacaccggca caacccatcg atgggttctc ccagttgtct tcaacaaagc
     2941 gaatatcgtg ttctaatgga tcaattccta aaagacgcaa gctttctaaa taaagctctt
     3001 ggatattttc aggtgaaggt ttcattacga cttgaaattg atgatgctga tacaaacggt
     3061 ttgggttttc tccataacgc ccgtcagctg gtcggcggga tggctctaca taagcagcat
     3121 tccatggttc cggtccgatt gcccgtaaga atgtatatgg gctcatcgta cctgctcctt
     3181 tttctgtatc ataagcttgc atcagcatgc agccgtttga tgaccaaaat ttttgtaatg
     3241 ttaaaatcat atcttggacg gtcaattttt tactcatctg ttttgcccct ttcaattttt
     3301 aagcgttgct gtcaataaga atacaggaaa gaaaacgaca aaaagtccct atgtctgatt
     3361 acagacatag ggacgaataa tttcgcggtt ccaccctact tccgaataaa gattcggcag
     3421 cttcatttat cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg cttccactct
     3481 cccgaactcg ctaaaccacg tgttttttac tgatcttggt caatgtacgt attcattttg
     3541 cagttttctg gtttgtataa tagatcctct cagtgaacga tctatttaat ctacggttaa
     3601 taatagacgg aagtccctgt atttgtcaaa tagttttgat ttttttgttg tatctttcat
     3661 ttattcttct ttttcaggta tctctttttc aggcatattt ttctctggcg tctttaatac
     3721 gtgtgcccaa gacttcattt gatcaatgaa ctttttgctt tttagatgaa taccgacata
     3781 ttcatcatac aactggtcaa tgaggtcacg gattgcctgc ttcgtttctt ctttcaagtg
     3841 gattccactg atcttatcat aagaaatagc cgaaaacatt cgaacgaaat aaacagcgcg
     3901 aggatctgca tgataacggt gttcgtccat tggccagtgt ttctcgcata tcacaccgcc
     3961 atatttggaa gaatagtcaa attttccttt tgtttccccg catacgacac agtgcgtcca
     4021 aattggcgcg attccgaagc gttgcagtat ttgcacttca aagatattcg tgatgatttc
     4081 tgcatcatct ccttggtcca tccgctgcag agcttgttgc gtaaattgga ataaatgcgg
     4141 gtcatattga tgatcttcga ttgctgcatc tactagattt agcaaatacg taccataacc
     4201 gctcaaaaag atatcttctt ggatgttccg aaacgcatgg acgtctttga cactgcttaa
     4261 aaacgacaaa ccttcacttc gaaaattacc gatatagact gcttcggtaa aggggagcaa
     4321 agccggagct aatggctgat tttgccgatg gattcctttg atataaaaca tcattttgcc
     4381 agaagattcc gtaaagattt tgactaattt atctttttct ttgtaattac ggctagataa
     4441 aatcaagcct ttcgattcta caagctggct catattctcc ctcctaaaaa ataaatctgg
     4501 aaaagcagat tctgccgatc cagattattt atgtttcgat cttccgctgt ttttctcaac
     4561 gtacggattt ttatccagtt tatttctata cctaacctat aagaagtcca gaatatcagt
     4621 actcgtcttt tcggtagccg aagtcttgca gatagacttt tttgtctcgc cagtcttttt
     4681 ggactttgac ccaaagttct agaaatactt tgtctcctaa aagattttcg atatcctttc
     4741 ttgctttcgt tcctacttgc ttcagcattt ttccgccttt gccgataatg attccttttt
     4801 ggctatctct ttcgacgatg atcgtggcct ggatatggat tttatcattc tcgtttcgct
     4861 tcatcgaatc aacaacgact gcgacagaat gaggcacttc gtctcgtgtt aaaaacaaga
     4921 ctttctcacg gatcaattca gaaacgatga agtattcagg atgatcagtc acttgatcat
     4981 ctggaaaata ttgtgggccc tctggcattt cactgactaa tacatccatt aagcgttcga
     5041 cattgtttcc ttctgtagca gagatcggaa tcacttgtgc aaaaggcatc tgcgtcgaat
     5101 aatcttcgat gattcccagc aactcgtctg gatggatctt atcgatttta ttgataatca
     5161 gataaacagg agaatgcacg ttttttagac gttcaataat aaaatcgtct ccccggccac
     5221 gtttttgatc agcactgatc ataaataaga ctgcatccac ttctttcaac gcactgtaag
     5281 ctgcttctac cataaaatct cctagacgat gctttggttt atggattcct ggtgtatcga
     5341 taaaaatcag ctgagcttct ggtgtagtat atactccttg gattttattt ctagttgtct
     5401 gtgccttgtc actcataatg gcaatttttt gacccacgat tcggttcaat aatgtggatt
     5461 ttcctacatt cgggcggccg acgattgcga caaaccctga tttatgttct ggcatatacg
     5521 ttctcctttt taattaaaca acaaatgata tattttaggg acaaataaaa tcaacccaat
     5581 gatgacagca aaacaagcgg tcactaaaac tgctccagct gccatatctt tgattttttt
     5641 cccaatcgga tgaaaatgaa aatccgtaaa catgtccact acattttcaa aaacagtatt
     5701 gatgatctct actatccaaa cgagaaaaac agctaaaagc agccataacc actctatccg
     5761 atcaagctgg aaaatcacgc ccataaataa agcaaggata ccgaacgcaa catgtttcct
     5821 catattgcgt tcttcctcaa aaacggtttt gactccttgg acagcaaatt ccacagacat
     5881 caggaaatgt ttgtttttct ctacgttttt atcttttgag tccataggca tccaagatct
     5941 ctttctgtaa accaaacatt tctttctcat cttctggtgt catatggtca tacccgttga
     6001 tatgcaaaaa tccatgtacc gctaaaaagc caagttcccg atcatatgaa tggccgtaag
     6061 cttctgcttg ttcagcagca cgttcaatag agatcatgat gtctcctagt acacgaggta
     6121 atggattctc tccatcttca aaaatcactg ggatttcgtc ttcgccttct tcttccagag
     6181 caaaactgat gacgtctgtc ggtgcatctt ttccacggta atcacgattg attacttgaa
     6241 ttgccgcatt gtccatgaaa gtaacagaca tttctgtttc ctctggcagc tttaaataag
     6301 atgctgcaaa ttgcagcaag tcatcgattt ctttcatttt ctcttctgag acttggtttg
     6361 tttcatccat aaaggtaata tccattatac atcgcttctt tctattcttc ttgtacttcc
     6421 tttatcttct caacttctgt tttcatttta ggatatttga tccgagaatg gaaggtgcta
     6481 cttaaaccgc tgattaatga tttttccact tttcggattt ctttcaaggt cagtccacta
     6541 tcgtctaatt gaccatcgga aatacgctct tcgataagat tatggacaaa actttcgatt
     6601 ttttcgctcg tcggatgatc cattgcccgt actgcagcct cacaactgtc agcaatattg
     6661 acgatccctg cttcacgact ttgcggtttt ggtccaggat aacggaattg ctcttctgtt
     6721 acttccggat tgcgttcttt cgctttgatg tagaaataac gcattagcgt ggttccatga
     6781 tgctgtttgc aaatatcgat caccatttgc ggcatattat aatcttccaa tattttggct
     6841 ccatctgtca catgtccaaa gataatatgt ttactgtctt ctggcaataa gaaattgtgc
     6901 gggttttccg ctcctgaagg caagttttca acgaaaaagc tagcatgttt gatcttgccg
     6961 atatcatgat aatagcaagc tactcgagtt agcaaagaac gtccgccgat ttctgctaca
     7021 gcgttagcac ttagattagc taccatcata ctatgatgat aagtacctgg cgcttcttcc
     7081 agcaactgtt tcaataaagg atgatttgga ttgctgagtt catttaatac aattacactg
     7141 tcatcattga ccatcaattc aatatatggg tgtagaccga tcgtcagcaa gaatgagaag
     7201 agtgcaccag tgaacccgca gacgagcatt aaccaagtcg ttgcatcacc aaagctcatt
     7261 ccttgataga cgatcaaaat gacatccatc atcacaggaa aaataatgac ccacatggca
     7321 gcagataatc cttgttctga gatccgtttg cgtctgacaa tcgttgccat gagcccagag
     7381 aaaacataag acatcaagat aatcgttaga aagttcgtgc caatcgaatc ataaaaaatg
     7441 aaaagtgcgg atactgcttg gaaaatacca gccatgatcc ctgctcttcg attcaagaaa
     7501 aagcttaaaa caagtggtgc aaaagcagcg ggataaaaca gcgggatata agtagcttgt
     7561 tccgtttgga ataattggaa aaatttcatc agcactatgc tgactgacat agctgtcaca
     7621 tagaaaagca cgtattttgt tctttctcct gcattttgga attgaagtga attataaatc
     7681 agtacgccga tttgaagcaa aacagctaaa accatggcaa ttagcggaaa aatcgaagtt
     7741 gtttgattag tcagtcctag taattccaat tttttcatag ctgaagcatc gatttgatta
     7801 ccttcacgaa caatgatttc tccttggaag atcatcactg gttgtacagc ttctcgtgcg
     7861 gattgtttca attcttctgt ctttttctca ttcaatacgt cattcactac gataccttga
     7921 tccactaagt agcgaatcat ttgttgttgt gctggtgtga cattcaaata ttggatttgc
     7981 tcttctgctt cttgtttaaa agcttccaat tcactttcac gaacttgttt gctcatctgt
     8041 tcgtcaatga gcttcagact ctcatccctc acagtatgaa tctcagtttc agtcatttca
     8101 aatatcgtcg aataaaaatt gttcggtagt ttttgataaa acacaacatt ttctgcattc
     8161 acattttcaa aatttttctt cagcgcggcg atccgctctt ctgtagtagg ctttgggata
     8221 ttttcatctt cctttgcctt atcaatcttt tcgtcataag atttgttgat ggcatcattc
     8281 gtcttatcga tcaattcaaa tagttgcttg atccgattgt tctggtcatc tgcaagatct
     8341 ttttggtaag tgtattctgg agtgactgct tctgctgcca gttttctttt ttgttcggtt
     8401 tcttccgtgt tctcgatcgt tttgttcgca cggatacttt cttctgctac ttggccttct
     8461 ctgaaatcat tggccttttg tttgacacta ctaaatgtaa cgccgcagat aatgataaaa
     8521 aacaagaaca gcaggaaagg caatgctgcc tttccaagtt tttcttgtag tttctgcagt
     8581 gttttattca acatgtgctt atcctcctaa gcaaagctta ttcttgatgg agatctgcct
     8641 tttcataagc tttgatgatt tcggcaacta cagggtgccg aacaacgtca cttgcttcaa
     8701 aattaacaaa atcgattttc ttgatttgct ttaatgttcg ttctgcatga acaagaccac
     8761 ttgtcgtccc tcgaggcaag tctatctgac ttgtatcccc attgacaatc atttttgatt
     8821 gataaccgag acgggtcaag aacattttca tctgagcgac tgtcgtgttt tgtgcttcgt
     8881 ccaagataac gaatgcatca tccaatgttc gcccccgcat gtaagctaaa ggtgcgattt
     8941 cgattacgcc tcgttccatc aatcggttcg tatgatccaa tccaaaaatt tggtataaag
     9001 catcataaac aggccgcaga taaggatcca ctttttcttt tagatccccg ggaagaaagc
     9061 ctagattttc acctgcttcc acagcaggcc gcgtcaaaat gattttttgc acttcacctt
     9121 ttttcaaggc agcgattgct aatactactg caagaaaagt ttttcctgtc ccggctggtc
     9181 ctacgccaaa tacgatatca tgtttggcta ccgattcgac atatttcttt tggccgctgt
     9241 tttttacccg tataggtttt ccattacggt ctttgacgat ctcttcctca tacatctcaa
     9301 caaaataatc taatgtacct ttttgcccca ttttcaatgc cgtcacaaca tcgggcgtac
     9361 ttacgtggat tcctcgtttg atcaattcct gtaatgtccg aatgactgag gcagccaagg
     9421 aaatctggga ttgctcacca atgatctgaa tgatttctcc acgtgtatgg atggttgttt
     9481 ccgttgtttc ctcaatcagt tttatatgct tgtcgtgtgc acctaaaagc atgctgacat
     9541 catcagcagc cgtcaatcta atctctaaag aactagactc ttccgtcaaa aatagacatc
     9601 cccttttttt cgattaattc tttcctattt taccatactc agaagaaact atatagcaaa
     9661 taagaaaagg ttctaaaaga gttgcttaac cataagtacc aagaaacgtt aaaaaactag
     9721 ccgcttccat tctctaaagg tctcgtttga ataaaaaaag ctgatcatgt aagctatgca
     9781 taagatagag gacaagtatt ccgtctaact tagcaagtat tctctgggta tttttcttgt
     9841 ttcaaaaaaa gaaaacaaaa tatttgaaat attcttgcat ttcaaaaaaa acaggtgtat
     9901 tataacgtca acaaataaat atacagcaaa gcgtcgagga aaacagtagg ctattcagtg
     9961 atttattccc agtgagtcat gtaagtgaga aatgacaata aatcgaatag acgaacatca
    10021 tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc ctccattatc aggctagcgg
    10081 ataactttat cgaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa aaaggtggta
    10141 ccgttctaga atctagagcc cttttcccaa gtggaaaagg gcttttttta tattttaagg
    10201 tggtggtttt catggaatct gatattagtg gtatttctag tttctgcaac gtttattttg
    10261 ttactcaaaa tgataattat tggaggaaaa atttatggaa gaaacaaaaa cttattcatg
    10321 gaagcaatta ttgatggtca gttcacttat cttcgggatg ttcttcgggt caggtaatct
    10381 gatctttcct gtacacttag gtcaaatggc tggatcaaac tggatcagcg ctggtgtagg
    10441 atttgccatc tcaggcgctc ttttcccatt attagctatc ttagctgtag tagtgacaaa
    10501 aagtgatggt ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg ccttgttctt
    10561 gatccttgtt catttaacga tcggaccatt ctttggtaca ccgcggacag cagcaactgc
    10621 ttttgaaatg gctgctaaac cctttttgcc tgaaaaatat gattcattag gtatgttttt
    10681 attctctgct cttttctttt tacttgctta tttccttact gtacatccaa cacgtttggt
    10741 caaatatgtc ggaaagtatt taaactcctt gtttcttgca ttattagcta tcatttttgt
    10801 cgtttcttta ttccaaccca tggggaatct aaaccaatct gctgcagatt cttatcagac
    10861 gaacgctttg acaaatggcg ttttagaagg atacaacacg gttgatgcag tagctttact
    10921 tgctttaagt gtcacttttg tccatgcagt tcgtgatctt ggttttaaag ataaaaaagt
    10981 cacagaatta acagcaaaag ctggtagcct gagcgttgcg ttagaagtca tgatttattt
    11041 tggtctggtc cttctcggtg cgatgagttt gaataagatg tctttatcag aaaatggcgg
    11101 aactgcactt tctcagatca ctaattttta tttaggtaac ttcggtgcta gcttcttagg
    11161 ggtaatggtc acattaggtg tcttcactac tgctatggga ctcgttgttt cttttgcaca
    11221 agatttccat aaactgttcc ctaaagtcag ttatatgaca tggctgcggt taacgacttt
    11281 tgtctctttt gtcgttgcga atgccggatt ggataatatc atccaatggt ctttaccagt
    11341 attgatgctt ttgtatccat tgtcacttgc gttaatctta ctttccttaa cagcaaaatt
    11401 cttccaaaag agtccatttg tctatcaagt aacgatgttc ttttctgccg ttccagccgt
    11461 attggatatg ttggcaaatt cgcctgcatt agtgagtcag caaactgtcg tagcaagctt
    11521 actaggattt tatcatcatt atgttccttt tgctgcatta ggtcttggct gggtcgtccc
    11581 aacattgctt ggttacgctg gcagcttgct cttttattat gtatatcgcg tatcaggata
    11641 taaacaagaa gcaagtgagt taccagaaga ataaccaaat caaacacaat ttaacataag
    11701 agatattaaa caggaaaaaa acagctagaa gaaaga
//
LOCUS       DACDIP010000074        11622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDIP010000074 DACDIP010000000
VERSION     DACDIP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.074"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(117..1247)
                     /locus_tag="IV257_002321"
     CDS             complement(117..1247)
                     /locus_tag="IV257_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8815414.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1788..1994)
                     /locus_tag="IV257_002322"
     CDS             complement(1788..1994)
                     /locus_tag="IV257_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815415.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2010..2624)
                     /locus_tag="IV257_002323"
     CDS             complement(2010..2624)
                     /locus_tag="IV257_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8815416.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2771..3454)
                     /locus_tag="IV257_002324"
     CDS             complement(2771..3454)
                     /locus_tag="IV257_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8815417.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3563..4021)
                     /locus_tag="IV257_002325"
     CDS             complement(3563..4021)
                     /locus_tag="IV257_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8815418.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4105..5868)
                     /locus_tag="IV257_002326"
     CDS             complement(4105..5868)
                     /locus_tag="IV257_002326"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8815419.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6089..7549)
                     /locus_tag="IV257_002327"
     CDS             complement(6089..7549)
                     /locus_tag="IV257_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815420.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7757..8683)
                     /gene="glsA"
                     /locus_tag="IV257_002328"
     CDS             complement(7757..8683)
                     /gene="glsA"
                     /locus_tag="IV257_002328"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8815421.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8706..10133)
                     /locus_tag="IV257_002329"
     CDS             complement(8706..10133)
                     /locus_tag="IV257_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8815422.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10164..11378)
                     /locus_tag="IV257_002330"
     CDS             complement(10164..11378)
                     /locus_tag="IV257_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8815423.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3521 a         2404 c         1916 g         3781 t
ORIGIN      
        1 atatgatttt ttgagatact ttcattgcga attcctcttt ctaaaagaac ctctcgcttg
       61 aaaaataaaa gattgtactt tatgataatt caaactgaga gtttctcagt ttctctttat
      121 tgtttttcag gtcgatagag cgccaactct tcggcctgta tctttgaaga aatattcaac
      181 gtgattccga ttcctaaaga aaggatcaga taacttgttc ctccgtagct aacaaatggc
      241 aatggcacac cagtcattgg catcagcccc gcgatacttc ccacgttcat tatcgtctga
      301 acgaatagca aagttccgaa tcctaaaaga aacaatccag cttgctgatt tttacagcga
      361 ctgcttaaac aaaagattct catgcataat gagaacaata agaaaagcac acacaatgca
      421 ccgattagcc caagctcttc tgtaataatc gaaaaaataa aatctgtttc tgtctctgga
      481 agataccctt tttttgtgat actatttcct aatccccgcc caaatatgcc accattatat
      541 agtgcataaa acgaatgggt catttgatag cccgcaccat gactatcgat aaaagaatct
      601 cttaaagtcg cgatcctttc ataagcatga ttaaacatct gtggaagcca acctttattt
      661 cctaaatata ataccccgcc tgctgctcca atcagcaatg cagaaaaagt aacgattaga
      721 tagatccctt ttttaaatgg aaccgccgct gcccaaaata tgacccacgc aatcgaaagg
      781 atcatcaatg ctccagcaat ctttggctga aataaaatca gaaaagttat acctactgta
      841 atcaggaatg gtttttttag atcttttggg ctactatttc cgtcacgaaa aaaccagctt
      901 aaataaaaaa tcaaaaaaag atttgccagt tcagaaggct ggaattgaat gccaaacaga
      961 gagatccaac gttgtgcgcc gttgacagtg acaccgaata tccctattct tactaatact
     1021 aaaaagaaaa tcgataaggc taaaccgtat cctgctattt tagggtgaag taaatagtgt
     1081 agtttgactg aacgagctaa gaagatcacg ctccaactca aaaatatgaa aattagttga
     1141 cgcaataata gactttttgt attttcatcg gcttgcaata aacgataaga actggcactg
     1201 tatacttcta aaagaccgat catggacaaa gtcaaataag gacccaaaat ccaccaatcg
     1261 atcttttttc accgcatctc ctcctcacac agcaatccac ttgtcaggtg aggagcgagt
     1321 ttgtctcatc aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa ccttttcgaa
     1381 caagaattct gacctagcat catctcatcg tccgagtttt agcactatac aacgtaaaga
     1441 aaacattctt agctattgga aaaagcctta atccaacagt ttctgtttta cccattggcg
     1501 actttcgacg tttctgggca acagcctatg tttgtatagg agcctcacct aacaaggtta
     1561 ttactatatt ctatttacga gttcatgata gacttttcct aatgattagg caatggaatt
     1621 tgaaatgaaa tagtgaaatt taacaatctt taatattttt tagaaaaaaa taacaaataa
     1681 gttgaaagaa tcccgttgca aattctttca gttgcaccag gattctttga taatttttta
     1741 gttttatgtg tttttttata tgttttttca cttttcggaa taaatattta tttgttaaag
     1801 taattaaaca atccgctaat cgtttgatct ggcatttcta tttccttacc atctaaagta
     1861 acagtagtaa cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg cccttgcaga
     1921 aaatcattca ctgtttcata ttcttttttc atttcttttg aatctgtcga tggtttatag
     1981 cttattttta acataactag tgctcctttt tattcgggac gtgcaatcgt gaaaatttct
     2041 cctattgctg cataatcatt tcctgccaaa cggcttacag cattcaattt tcttggatcg
     2101 atccttcctg tcttatcatc atagactgtt tcatttaaat gatatttctg tacttctagc
     2161 aaaaataaat cagctgttac tttattttta ttccgtatct cgacatgttc atataaggat
     2221 gtttcaaaac gaacttttgc ttctgtcaat gctggtactg aaatgattac tgaatcagtt
     2281 aaagtcaagt cagtcaggta cagttcgctt tcctcattag gcagattggc tgctgtttta
     2341 tttacttctt ctacgttgtc ctcatcaagg atatggataa cagcttctct tgtctcaaga
     2401 atattctttg ctgtgtcttt tactgcatct tgttttctct ggatcgccaa ggataagatg
     2461 ggtcgattcg aactgacgat attgaagtaa ctgaagggtg cgatattcac tatatctttt
     2521 gctgatttag tcgttactac tgctactggt ctagggatga ctgaaccaat cagcaacttg
     2581 taattttcac gttctgtcaa ttctgaagga ctccgttcaa acatggtttc gccttctttc
     2641 tataaaattc gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc ttttttcatc
     2701 ttcttagaat ctgctttaaa ctatctatca atttttcttt tttgtcaaga atctgtgccg
     2761 tgcgtgtttt ttagttattt gctttacttg cggcttcgac atatctttta gtgatcaatt
     2821 gcggacgggt gataatagat ccaactacga ctgaaaaagc acctaattca tatgctttct
     2881 ctacttgttc aggagagtca gtatgtcctt ccataattac tgggatatcg acttgttcta
     2941 tgatttgatt caataaatca aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc
     3001 ctgctaaagt cgtagacaaa agatcaaaac catcttcttg cgcttgaagc gcttcctcaa
     3061 aagtcgaaat atctgccata gctaaccgac cgtgttcgtg aatcatttgg acaagttcag
     3121 cagttgtttt ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg atttctgctt
     3181 gcgtctccaa aagttcttcc acttcttttt ctgtcgcagt aataaacact tctgacccat
     3241 cgtaatctct tttgataatc cctattaccg gtaaatcaac gattcgcttg atttcattga
     3301 tatcttctgt cgtatttgct cgaataccaa ctgctccgcc ttcttttgca gccaaagcca
     3361 ttctccccat aataaaagaa ctgtgtagcg gctcgttttc caaagcttga caagaaacga
     3421 cgactccgcc tttgatttgt tccagtgtat ccacaatatc tcctccagta atgttatata
     3481 aaaatacagc ttcatataaa aatgtaaccc ttttctattc aggagacaat aatgagctat
     3541 ttctttcagc agacctttct tactaaaaga aaattacttc aaattcttcg tatacgacca
     3601 gcttctcttc tgtaaattct atgttcaacg cttctttata agcatgttta aaaaactcag
     3661 catggacttt ccgccaataa gctaatgtcc gatccccttc tccttctttc caagcatgct
     3721 ctttcgtgac ttcactaaaa ggcaagatgg tcacatttat atttttgatg atacaaactg
     3781 gttcattttt tccattcagg accacgtcta attgaccaac ttttggtaaa ggctcattct
     3841 cttgtttata cagatcgtaa ccacttgatg taccagtctt tatctttttt acaactagtt
     3901 cgctcaactc gtctgccatt ttttctgtat ccccaaatga aaagaaacct aatttttctg
     3961 attgataact aggatatttt tcttggaact ttttgacata ttcttctact tgctgataca
     4021 taacatccac ccctttgctt tttataatga aaaaaccatc cagatactag tataaacaat
     4081 tatctggatg gtgagcataa tgaattattg aatattcaag acacgcagac tatcttcaac
     4141 gatttgtttt ggtgttaaat gataacgttc ataaagctgc cctacaggaa cgctatccgt
     4201 gaattctttc tctgcaccaa aatttaatac gttcattgaa gaatcaccat aataacgact
     4261 gatattttcg ccaaatcctc ctgacaaact accatcttct aatgtgatga ctaattcgtg
     4321 tgtttctttt aatttatcta atgtttctac gtcattttct gtgatcgttc ctggattgat
     4381 caacgttgct tcgattcctt tttttctaaa agcttttact gtttcttttc ctaattcaag
     4441 aaaaccgcct aaagctaaaa ttgctacttt tcctccatta tttactgttt gataggctgg
     4501 aatactataa tcatttgcga tagctgtacc atgttctaca ccatgtgtag gaattcggat
     4561 tgctacaggc tgcgctgttt gttcgaacgc ccaatctagc atagagatca tatcctcttt
     4621 tgttgctgga gcaagatatt tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt
     4681 tccttggtgt gttcggtcac tagctgagat cgttccgcca ccgactaaaa taatcactgg
     4741 attctcgttg atcgctaaat catgtgataa ttgatcatat gcccgttgca agaaagtact
     4801 tgattggaag acgactggac gtactccctg agaagccaaa gcagaagcga aagtgataga
     4861 gtgctgttca gcgatcccca cgtcaaaata atgatctgga tgtttttctt ggaatgtttt
     4921 cagtccaaag gcgcctggta tcgctgcatt gactgcggca ataggagctg tacttgtttg
     4981 cattttctta tccaaataat ccatgatgat catattaaat gtttctccag aagttacttg
     5041 ctttgcctcc ccggtggaca gatcaaatgg tacatgccaa tgatatttca ttttattatc
     5101 gattgccggc tgataaccgc gacctttttc cgtatggacg tgaagtacaa tcggatgatc
     5161 aatatctttt acttcttcaa ataaacggat caccgtttcc agatcgtttc cattatccac
     5221 gtaacgataa tcaaatccca ttgctttaaa taggttattg ctgcttttac cgttggtttg
     5281 acgaagctca gctaagtttt tatataaact gccatgattc tcatcaatag acatctcatt
     5341 atcgtttact aatacgatta gattggattg taaaacacca ccattgttca atccttcaaa
     5401 agctaagcca ccacttaggg agccgtcacc gataaatgct accacatttc ctgattcttt
     5461 cttcagatct ctcgctttag ctaaacctgt tgctaagctg atagatgtag atgtgtgccc
     5521 cactgtaaaa tagtcatgtt tactttcatg ttggttggaa tatccgctca cttcacggta
     5581 atgtcctttt tcaaaaccat tttttcgacc agttaaaagc ttatgaccat aagactgatg
     5641 ggaaacatcc caaacgattt tatcctttgg tgaatcaaat acataatgaa acgcaatcgt
     5701 taattctact acacctagat tcggaccaac gtgcccacct acatcgctgt ccttttgcaa
     5761 gattttttgg cgcatctctt ctgccaactg tttcatttcc tcaacagata attttttgac
     5821 atctgcgggg ctattgattg catccaatac atgtaatact gtactcataa tacattctct
     5881 cctccgtact atcttctttt aatgaaaata gatttgttac actttatcta ttctactact
     5941 tagagttgac tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa
     6001 gtagatcttc gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt
     6061 tgtagaaggt ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt
     6121 cctgagcaat atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg
     6181 tcttttcagt ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt
     6241 tcgcacatag acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt
     6301 cagcgatgcc ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg
     6361 aactttctga aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca
     6421 aagaaagact tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat
     6481 ttttcttttt ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt
     6541 ctgatagaag atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga
     6601 caaattgtaa aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac
     6661 taataatctg taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa
     6721 caacttcgaa atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga
     6781 acaaagaaaa aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt
     6841 ccaatccttg acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt
     6901 ctttcagtcc aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa
     6961 aggacttttc atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca
     7021 actcgttaca agcttcttct acatacggat ttttattttc atagccttcc caattgacat
     7081 aacgtactaa agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat
     7141 ttttcaaata atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt
     7201 tgctcaaaga aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat
     7261 aataacttaa aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa
     7321 gagcaatacc atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta
     7381 tacaatattc tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca
     7441 cccttagaga aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac
     7501 acagatacac ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac
     7561 gcctccagtt tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct
     7621 ttttatttag aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa
     7681 caagctcaca atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag
     7741 cctctttcaa gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca
     7801 acaattggat accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta
     7861 tgccgtaacg atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta
     7921 caccaacatg gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg
     7981 atttgacgat tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc
     8041 ctttatttgc cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc
     8101 attgtctgaa ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt
     8161 tcatatagta agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt
     8221 cttcattcaa tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct
     8281 caaatttttc atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat
     8341 taacaaaagg attcattgga tgtttgcgat gattgatttc catattcatg atagaattga
     8401 aggcaaaacc ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt
     8461 tgattgctaa taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg
     8521 cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt
     8581 tgggatccac ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt
     8641 ggaaattttt tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc
     8701 attttttatt tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata
     8761 actaagacga taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct
     8821 gcgatactag caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct
     8881 aaacaagcaa cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga
     8941 ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg
     9001 tagattacaa cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt
     9061 aatgtaaaga cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca
     9121 ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct
     9181 acgtgaagtg cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa
     9241 caaacaagaa tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat
     9301 aggaattcta acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta
     9361 gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac
     9421 attgctaatg gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt
     9481 tctacgccca tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa
     9541 aaatcaggaa tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca
     9601 taatggatcc ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg
     9661 atccgagcaa tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag
     9721 aaaataatca ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc
     9781 gttgcatagg ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc
     9841 tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc
     9901 caagtataga caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat
     9961 gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct
    10021 gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa
    10081 aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt
    10141 cctcttttct atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat
    10201 ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa
    10261 atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc
    10321 cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct
    10381 atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca
    10441 tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc
    10501 ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat
    10561 ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca
    10621 atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg
    10681 actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa
    10741 agcagtcctg taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa
    10801 ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg
    10861 tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc
    10921 catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata
    10981 tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg
    11041 ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg
    11101 ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg
    11161 ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc
    11221 ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg
    11281 ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg
    11341 aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga
    11401 ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg
    11461 catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc
    11521 acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc
    11581 tttttttatt tttaaagata ttttttcata aggaataaag aa
//
LOCUS       DACDIP010000075        11597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDIP010000075 DACDIP010000000
VERSION     DACDIP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.075"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            261..467
                     /locus_tag="IV257_002331"
     CDS             261..467
                     /locus_tag="IV257_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8815424.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(669..1670)
                     /locus_tag="IV257_002332"
     CDS             complement(669..1670)
                     /locus_tag="IV257_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8815425.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1675..3588)
                     /locus_tag="IV257_002333"
     CDS             complement(1675..3588)
                     /locus_tag="IV257_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8815426.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3756..4262)
                     /locus_tag="IV257_002334"
     CDS             complement(3756..4262)
                     /locus_tag="IV257_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8815427.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4422..4862
                     /locus_tag="IV257_002335"
     CDS             4422..4862
                     /locus_tag="IV257_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8815428.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4888..6045)
                     /locus_tag="IV257_002336"
     CDS             complement(4888..6045)
                     /locus_tag="IV257_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815429.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6048..6404)
                     /locus_tag="IV257_002337"
     CDS             complement(6048..6404)
                     /locus_tag="IV257_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815430.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6702..7676)
                     /gene="sgrA"
                     /locus_tag="IV257_002338"
     CDS             complement(6702..7676)
                     /gene="sgrA"
                     /locus_tag="IV257_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8815431.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7872..8711)
                     /locus_tag="IV257_002339"
     CDS             complement(7872..8711)
                     /locus_tag="IV257_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8815432.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8896..9783
                     /locus_tag="IV257_002340"
     CDS             8896..9783
                     /locus_tag="IV257_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8815433.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10377..11072)
                     /locus_tag="IV257_002341"
     CDS             complement(10377..11072)
                     /locus_tag="IV257_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8815434.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11056..11454)
                     /locus_tag="IV257_002342"
     CDS             complement(11056..11454)
                     /locus_tag="IV257_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8815435.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3455 a         2311 c         1737 g         4094 t
ORIGIN      
        1 gatacatcga atttttgggg aaattctttt gggggtatcc taaatctttt ctattatatt
       61 cccatatttt ctattattgg tctagacgta tgatggtaaa ctttgtacct atctgattgc
      121 tgaatcatta attgcttatc attagccatc tttgtttcaa gttcgctaga tgatcatttt
      181 attcacatcc taacaaactt gtcacattta tagattctta atctatttat catacaatta
      241 taaaaaagga ggtgctgctt atgaaagcta tccatatttc tttgcttttt ttcgctagtg
      301 cattgatttt atttgctatt catctttgtt cccagcctta tatcaataac aacggaatat
      361 tagtggaacc agcattcttt tgcttaccac tcggcttttt gtcccttctt gcctccgcag
      421 gcttcggatt tgttgctttt tttaaacgat caaaacaaca aatctgactt ataaaattta
      481 cgtaaaagag atcgtgacaa taatcgtgtg atattcccaa atttcgaata aaaggtgccc
      541 cttgccatct ctaactcctc agtcacaagt tacaatattc ataaaccatg aaaagcttgt
      601 ttattgaaat tgctccttgt gacttggagc taagtgacag attacaacct cttttgcatc
      661 ataagacttt actccttgaa aaacattact aacttttgat atgtttcttg cgcttgttta
      721 ttgctgaaat caaactgata ttcatggttc aaaacagcat ttttctccgc ataatctact
      781 tttgtgactt tcacccccgc tttctcaaat acagaagtta cttcttccat ttgtttcgta
      841 aacgtatttg tatttccatc cgttaagaat gtgttttgcg tccaacgatc cgcatgtctg
      901 accaaggcta aacttttgac cttctctgat tgttcaaagt ccttattatc aaagtaagcc
      961 catccacatt tttcataaag aaagctagag attttatcac ttacttgagt aagtgcagaa
     1021 ggatccaaca gaccagacaa agaaataaac ttcgtaatct gcttgttctc caaagtcgga
     1081 tcaagatgat cagctttttt aatttcttca ttttgaagtg ctaacaaata ttctccacta
     1141 atttgtgcac ctgccgaatt tccaccaatc gaaatattcg accaatctgc ttgatctttt
     1201 atctcttttt tagctgcttg aatggcttcg ttcaattgga taactggtgt aggatgtttt
     1261 gcttccggtg atcgttcata atctatattt acgatgattt gctgcgtgtc tgaacataga
     1321 tattccaaat aatcacgaca atcattttta tcccctccta catatgcgcc tccatgcatc
     1381 cagaaaagaa tcggtaattt tttcttttcc actttcggtg tgtagatatc catattatta
     1441 tttttgtact ttgaaggata ggatatttct ttttttatat cgatttgttc gattttttgc
     1501 tggaaatcat ctggtcgggc atatacttgt tctttttcac ttgtgtcaaa ttgttttcta
     1561 acaagcaagc taatcggctt tggtgtgatt tgattaatca aaaatacagt gactaaaata
     1621 actacgatga taactggtac tgcaatcact attcgcttga accatttcat tcatttaccc
     1681 tctctttctt cgccaaatcc atgtgataat cgtgctaaca gataaaaatc cacctgaata
     1741 aatgagaagc gaatgtttct tttctccagt tttcggcaac attctttttt ttgcatctgt
     1801 ctttttttgt actttcactg tcatctgaat cggttgcgta tcatcacttg attgatcctc
     1861 tcgaggttca ttctttccct tattgtctgc attctctgtc ggatcttgct tttttggtgg
     1921 agctactggg atttttttgt aatagaaaat aatagttggt atcgtctcat taaaaatccc
     1981 ttcagtcggt ccttcaacat ttgctaattc gtatccttcg aatgtctttt gctctgtcat
     2041 ataattttct ccataagctc cgtaaagctg gatttcgtcc tggtctttga gtagactttt
     2101 attgttttca tccacatact ttacgtttat ctgatcatgg aacactactt tcaaaaagtc
     2161 cgttacatgt ttcgtgtagg cagcatggtc aacttcctgt gcctttccat gccctgctcc
     2221 ttctactaat aaaagatctt tataccctcc tgataactca tataattttt gcgcgttaga
     2281 cacaggtact acatcatctg ctgttccatg gataatcaat ttaggcaatg aatctgacat
     2341 tccaatcgta ggtagagcag cttttgtatc caaatgcatc tcctgatcaa aatagtattg
     2401 tttcaaaagg cccataaata aatcttcatg ctcttgatcc ccaatgatgt ctaactgatt
     2461 ttttatggca tccggtagtg cgggggctac tgtctggctg tacgtaaaac gtagttgttc
     2521 atcaattgat gcaaatccgc aattttcaat aattcctttg acattgacag gaatatcttt
     2581 cgctaataca gacatcatcg tagctgctcc catcgaacca ccataaagta aaatcttttg
     2641 agatggccgc tctcgaactt cttgatttat ccagtaaagg acgtcatctg actcatattg
     2701 tccataggtc acataatcgc ctccgctatt accagtcgct ctggaatcaa caaataataa
     2761 attatacccg gccttataaa aatcattgta ttcttctgta acgatcccgt tattccaatt
     2821 ccctcgaaat ccgccatgga tcatgactgt tttatctgaa ccttgatcga cataatatgc
     2881 atatacatcc atcattttgc catcttgttt gtttagtaca gtttgatgaa gttgttttgt
     2941 atttggatag aagcctgtgt tttctgtagg cgttgtccca tcatctgtac ctccaagcat
     3001 cgacatcaac acactgtttt ttgcaaaagc gatatcgtac aatagataag tagcaatcag
     3061 atgtttgata atctctttat ctacaccata atcattaaca gcaagctcag tgactaataa
     3121 ctctattaat tttgacttga tgtcttctgt tggtccatta agtatttctg tcatctgttt
     3181 accgaagtaa ttcaaatatt ctttaccatt gaaattttca cctttaccga tatcatcatt
     3241 ttctgtccca ttctctttgt cacttacttt agcagaagag gattcgtcgt ttgacatttt
     3301 tgtagctgtt gcttcttctg tttgttggtt atctgtggta gcggtcgttt cttgatctgt
     3361 gctagtaaca gatgtctcaa ccgttgtttc agtatttgtt tctgtttgtg aagtactcga
     3421 tttcgtttca tttccagcat cagtagtttc tgtggtttct acctcccgag attccgattc
     3481 ggaagaaatg ctctctccat atactgtcca ttgcgggacg cttgtactta cagcaaataa
     3541 gatgaccaac gctcccttta atacttgttt tgttctcaag ctattcactc ctttttatgt
     3601 ttctaggaga attatatgta aacgcaaaca ttttttatac aaaaaataaa tggtttatcc
     3661 cataaaaata gaacaggatt attttctcat tttacgtaat aaagcagaat ggataaacca
     3721 gtatccattc tgcttttttt aaacgaagaa acaacttatt gctccaaaaa tgtcagaaat
     3781 cgtttatccc aaattttctc gtaaaaagcg attgtttcct ttgctttcat aaaaggatta
     3841 tcataagttg tcgttgctcc tctacacatc ataagctggt atcctaatcc atgagcaaac
     3901 ttcacagtcg tatctataca atactctgct tgtgccccac ataattcgat cgaatgacag
     3961 tccaattcag ttaataaaac actcaaatta gtcttataaa atgaattcgc atgggttttt
     4021 ctcacaaaat aactgtgctc aggaatagat aattccgaca atagctccca tggttttgtt
     4081 tgtggcacta attgagcatc ttcgtgttga acgaaaataa ctggtttccc aatcgtttcg
     4141 tatgttttga ttcttttatt cactaatgct gtcactgttt ctaattcgtg tatcggcgaa
     4201 gaatcaaaac ataccccttt ttgtaaatcg atcactacta ttgcgtcggc tgtgtttgac
     4261 atacttgtgc cttctttctt ttttcttgat ttaatagtat ttcttatcta gctagttcca
     4321 ttatacgtga caacattttc ttattttaca cctctaaatg gcacttttcg actatttctt
     4381 catgttatta tttcataaat acttagctat tggaggaatg tatgatgatt aaattgtata
     4441 caggttctgg ctgctcgtct tcaagacgcg caaaacgttg gcttatcgtc catgatatac
     4501 cgtttatcga acagaactta attcgtcaac cactaaccaa aaatgaattt atccacttac
     4561 taaaattgac aaacaacggc gtgaccgaaa tgctctctac tcattctatt agttataaaa
     4621 atcttgttca cagaatagat gaactcagtt tgagtgaact atatattgag attcgtacat
     4681 ccccttcttt attgaaaaaa ccgattatct acgaccaatc tgcaaaactt ttgactggat
     4741 ttagcgaaga taaaatcagg atgttccttt ctaaaactaa aagaaagcaa gagctcaacg
     4801 aactcttgct ttctggtcat cttgtcgatt ttaaagaaaa taatcttttt gcaatgtctt
     4861 aattttggca taaacaatct gcttttctta attaacgaaa tccaattttt ccaatatgga
     4921 tctatttttc tcattcctga tttttatgat ttcagctttc agacgcgccc aatcattttt
     4981 tgtaggttgt ccattccagt aaaatatctg tgtattcgtt tgatcatatg cctttttgtc
     5041 gataggataa tcgctaatga caatatcagg taactgatca gcggatgcac tatattttat
     5101 tgggcgttca gtcatagata atatcctttt ctttgcccat aattcctgtg ttttaccata
     5161 ctttgattga atatagaata tgacaggttg ttcgtgtttt ataaaaatat ccctgactaa
     5221 catacagtat tgaaaaatca accgtctgtt cttttcataa taagtattaa gctctggaat
     5281 tctgcgagct accccactaa aaaactgttg catgattgga tacatcactg gaaatatatg
     5341 ctgaaaatct ctgtcttctg ctgattttcc aaaagcatcc accttctttt ttgtcccaaa
     5401 aacccttgaa tacaaatgga tcattgccaa attgacatat agataatttt tttctgaagt
     5461 tgtaaaacaa atcttgaagt ttttttcaaa attttctaca atagacgatt caatccagcc
     5521 ccaactttct tgtactttat ctggagtatc caacaaatct tcttccaaat aggtgttcat
     5581 tacaccaaac ataaaataaa ggtaagcttg ctctgttgga gtgatgtgat tcatcgacca
     5641 gaagtttgct tcaaaaaaag gctctagtat gtaatgcgta aactcttctt ttgttatcaa
     5701 tggatgcaca aatttgttca tctcttctgg gacatacgta atcgagtggc cttgtttcat
     5761 tcgttgcaac gaaattctta gtaggctgat ccatttcttt ctatctatac tacgcgtata
     5821 tggcgcatgt ttcgcaaatt tttctaatag ttgacgttta ttttttatac ttgataatat
     5881 atcagaatga ttttcgtcga atgactgcca atacatcaag tgaaaaaagt atcggatacg
     5941 gtattcttct ccttctatcg gattaccttt tttcaagctg caagataaat gatagttttt
     6001 caaaatagta ttaagttctc ttaattttct ttgaatcgta cgcaaactta tataattttt
     6061 tgactaagat tttctaagga ttgataagta cctttgtaaa gtgaatccag taaaatataa
     6121 ccaatcgttc cctctaaaag tatagaataa actttactca gtaaaaactg agaagaaaac
     6181 tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg atacctttcg
     6241 caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta gtttagatct
     6301 aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata gttcaaaaat
     6361 tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca tactcctttt
     6421 tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata gattcgttac
     6481 tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt gctcttattt
     6541 tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa aaatagatct
     6601 ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt cactcaaaaa
     6661 tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac tcgatggaag
     6721 agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat ccatgtaatt
     6781 agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt atctgtttct
     6841 ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt tagcacactt
     6901 tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg taccaaatca
     6961 agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt gctactctca
     7021 ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct ttcgctgctc
     7081 gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct gctcgtgttt
     7141 tctgtgctac tttcactgct cgtactctct gtactgcttt caccgctcgt gttttctgta
     7201 ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt ccaactgctt
     7261 ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa atcaacttta
     7321 tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt atactcaata
     7381 ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc tgaacttcca
     7441 ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac gcttttgttt
     7501 ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg atcagttttc
     7561 acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat tccaatcatt
     7621 gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt cttcatttct
     7681 atcactctcc atataattac cttctatttg ataaaggaat tatataaaat gaaaatgatg
     7741 agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg cttgaatttg
     7801 tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat cagccttgct
     7861 ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt tttatatgca
     7921 tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat agatgcttca
     7981 gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt ggagaaaaaa
     8041 ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt ccaattccaa
     8101 gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag tgcgctgaaa
     8161 aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg attcaatccg
     8221 tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc aaatctaagc
     8281 atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac tgcaataaat
     8341 aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa ccgatacgcc
     8401 cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac aacgagacct
     8461 cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc tattcttttt
     8521 ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg tccgaccgta
     8581 ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag tgcattattg
     8641 acaagaattc ctaaaacaaa accaactaag actattaaaa cagatccaat caaccgaata
     8701 atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt ctttcttgtc
     8761 tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac gaactaagaa
     8821 tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg ttagactgtt
     8881 tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat gtgcctttca
     8941 gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat tgcaacgttt
     9001 aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa atcaaataat
     9061 caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata cggaaaagag
     9121 atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact cggtccaaac
     9181 tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa actctttaaa
     9241 cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaacaga taaagatcta
     9301 gacactgctt ggaaagaatt ctatccagaa gttagtacac agatcattct attttcgact
     9361 aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag gagaaaattt ctccaagctt
     9421 gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat gaattttgat
     9481 tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact aaaaaacggg
     9541 gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag ctattatcta
     9601 gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa atctgagcta
     9661 gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa aacaatccgt
     9721 aaagtgatga aaaaagacaa tgtaactatt aaagatcctt atgtaaaaaa tatattcaaa
     9781 taaacaaatt tgacccaatt ggggatgtgg caaaatttga gaagtgattt ttggaaattc
     9841 cttcttattt ttgaggcaaa ccacttttgc cacaacttca tcggggcctt tttttatttg
     9901 taccaaaaca aatcactgga tttgttacaa tcatgaggat ataatttaaa aaagtttttt
     9961 atttctatca catgaatcaa tgcgccatta agtagattct ttcatgcttc cctatttttt
    10021 cacttgccgt atcgcttcaa aaacaactat cataaactca aactcgactg aaaagttatt
    10081 ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc ttattctgtt atactagaga
    10141 aacacgaaag atatttttat gcgacacttt tcatttgttt ttatgaaatt tattcaaaac
    10201 tggcgtaact tcagagttac aaggatgtat tagaggttag ggaatacatc gtttaaatga
    10261 gaaataagta atagttagta atagtcaccc aaactttaaa ttaatgaata ttgaatcgtc
    10321 agatagcctc ctcgagtttt cgagaaggct atcgtttatt ttatggagaa attgattcag
    10381 aagtaatcag cgaccttttt tagttcacca atcgtttctg cttggaaagt ttgcacccaa
    10441 tcttcttgga agcccctcac tgctttatca atcgagggca tctctaatac ttgatctagt
    10501 agctctttac ctaacgttat cgcatgagct ccagatcgaa tggctgtagt gacttgttca
    10561 caatttttga aactagctgc taatattttt gttggtcttt gcgtacgttc gatctcagtc
    10621 cgcaacgaat caatcacttt catagcatca atgccttgat tttgcatcct gttataataa
    10681 ggtgcaatat aatctgctcc attattaata gctaaatacc cttgtaattc tgtgtaaata
    10741 gctgtaccgg taatcaaata accttctttt ttcaactttt tgatcgcttg aaggcctgct
    10801 tgattcaccg gcaccttgat aaacgtatct tttcccagtt cttctatgat aactcgagca
    10861 tcctctatca tttcttcttc cgttcttcct attgtctgaa tatggatagg aatctgtccg
    10921 attatttttt tgatttcttt catatgcgag aagaagtttg acgctccttc ttttttcaga
    10981 attgtaggat ttgttgttac gcctgttaaa ttcaaaaact gttgacaaga ataaatactt
    11041 tttgtatttg ctgtatcaag catgatttcc atatagcttt cccccattct gatcctcttg
    11101 atcgatttta atgattgtcc cctttcttaa atgcggcatt tccttatctt ctacatacat
    11161 tgttcctgga agttccggct catgagatcc atcaaatttt atcgtgatat gtcctaattc
    11221 attcaaatta gtttggacaa catctcctac tgctgtgatt acatacattt gtttatcaaa
    11281 acagagcttc atatcacaag ttatttcttt tcttacctga ttcaattcaa tcttataaca
    11341 ataatctgtt aatgtttccg gtgcttcatc tccaaataaa atcattattt tttctttcct
    11401 gaatacttct gcttctatac caacatctgt tacaaccgtt tcaaagatcg tcatctctgt
    11461 tcctcctcgt aaaatatcat ttgtctaata gtttttattc aaaatgtttc tagattataa
    11521 aatttattga ataacacatt cctaacggaa tttgtttcga tattgtgtga tttctcagag
    11581 tacaatggat gtatgga
//
LOCUS       DACDIP010000076        11511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDIP010000076 DACDIP010000000
VERSION     DACDIP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.076"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1030)
                     /locus_tag="IV257_002343"
     CDS             complement(<1..1030)
                     /locus_tag="IV257_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8815436.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLRDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IV257_002344"
     CDS             complement(1112..1801)
                     /locus_tag="IV257_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8815437.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IV257_002345"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IV257_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8815438.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IV257_002346"
     CDS             complement(2328..2831)
                     /locus_tag="IV257_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8815439.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IV257_002347"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IV257_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8815440.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IV257_002348"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IV257_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8815441.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IV257_002349"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IV257_002349"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8815442.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IV257_002350"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IV257_002350"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8815443.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IV257_002351"
     CDS             complement(6443..7462)
                     /locus_tag="IV257_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8815444.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IV257_002352"
     CDS             complement(7635..8522)
                     /locus_tag="IV257_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8815445.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IV257_002353"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IV257_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8815446.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IV257_002354"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IV257_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8815447.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IV257_002355"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IV257_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8815448.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IV257_002356"
     CDS             9753..10397
                     /locus_tag="IV257_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8815449.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IV257_002357"
     CDS             complement(10453..10956)
                     /locus_tag="IV257_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8815450.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3363 a         2384 c         2099 g         3665 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtccct taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg a
//
LOCUS       DACDIP010000077        11201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDIP010000077 DACDIP010000000
VERSION     DACDIP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.077"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(117..707)
                     /locus_tag="IV257_002358"
     CDS             complement(117..707)
                     /locus_tag="IV257_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8815451.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1230..1391
                     /locus_tag="IV257_002359"
     CDS             1230..1391
                     /locus_tag="IV257_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815452.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1410..1904
                     /locus_tag="IV257_002360"
     CDS             1410..1904
                     /locus_tag="IV257_002360"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP8815453.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            2542..3565
                     /locus_tag="IV257_002361"
                     /pseudo
     CDS             2542..3565
                     /locus_tag="IV257_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3908..4164
                     /locus_tag="IV257_002362"
                     /pseudo
     CDS             3908..4164
                     /locus_tag="IV257_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            4551..4778
                     /locus_tag="IV257_002363"
     CDS             4551..4778
                     /locus_tag="IV257_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8815454.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4794..5960
                     /locus_tag="IV257_002364"
     CDS             4794..5960
                     /locus_tag="IV257_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8815455.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6195..7238
                     /gene="hrcA"
                     /locus_tag="IV257_002365"
     CDS             6195..7238
                     /gene="hrcA"
                     /locus_tag="IV257_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8815456.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7332..7895
                     /gene="grpE"
                     /locus_tag="IV257_002366"
     CDS             7332..7895
                     /gene="grpE"
                     /locus_tag="IV257_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8815457.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7946..9775
                     /gene="dnaK"
                     /locus_tag="IV257_002367"
     CDS             7946..9775
                     /gene="dnaK"
                     /locus_tag="IV257_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8815458.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9926..11092
                     /gene="dnaJ"
                     /locus_tag="IV257_002368"
     CDS             9926..11092
                     /gene="dnaJ"
                     /locus_tag="IV257_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8815459.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3946 a         1791 c         2288 g         3176 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatccttgt aaagttgagt tttttatttt
     2101 aatgaattaa gactattaaa tagataatta ttgtaactta aaaaacacag ctaaaaatta
     2161 gttgtgtttt ttattaggtt tacttttctg gtataaaagg aactagctac tttttcaaaa
     2221 tttctaaatc attcaatatg tagcgcttac taaattattc gccataaaat tttataattg
     2281 gatcactcat tgttttacct aagtcatatt taagtaataa ccggtgtttt tacataagaa
     2341 gacttcgggt attttttagt ttgattagct ctgatatacc tataaagaat atgctaaggt
     2401 aagaatagta aaaattcaca tagatgaggt ataataatga tatttttata tattacagat
     2461 aacttgacac aaaatgagtt tttttcgatt tttcaagtta tctaatgcaa ttatattgat
     2521 atcattagga gctgcattgt tatgaatcca acattgatta ctaagaaaga attactaaaa
     2581 aaattagata tttctactgg tgtattagct aatttaatta gaaatggcat gccaaaagag
     2641 ggagaaatgt ttaatcttga taagattatt acatggcgag aaaattggtc taaaaacata
     2701 ttaggtgagt tagaagttgg tagagtatat actaacaaag aaatttcaga gaagtttaaa
     2761 tgttccaaac agggtggaat gagaagatct catcaaacta atactcttgt actgtttagt
     2821 gatcaaacag gaagtaatgt ttataaagat aagtggttaa atggtatttt acaatatacc
     2881 ggtatggggc taaaagggga tcaagtattg gataagaatc aaaataaagt tttagccaat
     2941 tcaaaaagta attttgtaaa gattcattta tttgaaacat ttaaaccaaa agaacacaca
     3001 tatctgggag aagtatattt agcaggacaa atatatactg ttaatgaaaa agatagttcg
     3061 ggcaacagca gaaaagtata taaatttcca ttagctttaa taaatcaaga gcaattgatt
     3121 gaagataaag atatataacc aggaagaaaa tcaaactaga catattcgta atttatctaa
     3181 tgctaaatta gaggaagaag caagaaaagt tagtaattat aataaaaaaa tggctgcaaa
     3241 atatgttaac gaagaggagt tttgcttctc aaaaacaaaa atatataaac gtaatccctt
     3301 cgtatctgaa tatgttaaac gacttgcaaa aggtatttgt caactttgcc aagaaaaagg
     3361 gccatttata aaagacggtg taccttattt acattgtcat catatagaat atttgtcgca
     3421 aggtggaaaa gatgtgattg aaaactgtat cgcgttatgt cccaattgtc atgctagaat
     3481 tcatgaactt gagttacctg acgataaaga aaaattgctg aagatcgttg aaaaaagaga
     3541 aaatagcttt tctaaaatac cgtaatttat ttttatagga caaataagct aatgtgagct
     3601 atgccttcta tataagacta agttctatta taagtaacga agtaattctc gttccttcct
     3661 gtagtaatat tttttaaata acctataggc ttcttcaaaa gtacatcctc cagtaaattc
     3721 taagtactgg aggattcttt ttctagtgtt gctttactca tgaaaaagta gttggctact
     3781 tcatcaaaat ttctgaactt ttttaatgta taataaagag ctcgatcatt tactacaagg
     3841 atgctagctc ctacattttc acagtttttt agcaagtaaa tttttctaaa atcacatcag
     3901 gaaatgcatg agattcttta agagaggcaa ttatagggct atgattacgg ttcgtgtaag
     3961 tgacgccaaa aaagaatggc tttcttatat ggctgaattt tatgggatta ctttatctga
     4021 acttctaaaa aactattcga tggaacaact agaagatgaa tacaatcgtc aaacggctga
     4081 aattgcccat aaacattgga taagaagtgg gaaaacaaca gtattcatgg aagaatcctt
     4141 aatgaatttg gtgggctgga atgacctgcc gtttaaaatt cacgcccgaa attcagaagc
     4201 agctctgatt ggcaaatacg tgtattggta gagatagaag ataatcaatt cgttgttatc
     4261 gctattcaag tagggcaccg aagaaatgtc tatgattaag agactgttta tttttacaaa
     4321 aaaataataa ttggaatcta gttaaaactc ttgaagtgag agatttgact agattttttt
     4381 atgaataaaa taaggacaaa attgtttagg aagaatcatg agtgtaaaaa aatcatccaa
     4441 gaagtcgatt cttggatttt ttagtggatt tgttcgccaa atacgtttag tatgcgtata
     4501 tgagaggttg taagcaaagc gttgtggtct gagcacttaa ggaggaatct gtgaaaatag
     4561 cttttcatat ttttgcagat tttgacttaa tgccgaaggt cagctttgcg attaccgttt
     4621 atcgagaggc tgtaaaagca ccgtgtagct ccgatgacaa taagcaagca tttttagagc
     4681 taggtttcct tgattctaaa tatgcggctt atttcaatga gttggtgctt gaacgcgatt
     4741 ttaagaaggt cgaaaacagg cacgactttt cgacgtaagg agaaagaatt tcaatgatag
     4801 aaacaataaa tcccgcagaa aacaaggttt ctgcgtacat tcatatccca ttttgcgagc
     4861 atatctgcta ttactgtgac tttaataaag tttttttaga agggcagcca gtagatgagt
     4921 atgtggaaat gctgctgaat gaaatgagat taatgctgga aagtcatcct acagcgaatt
     4981 tggaaacact ttatgtagga ggcggaacac caacttcttt atctgctaaa caacttgatc
     5041 gactattatc aggtgctaga gagatcctgc cttttcaaaa tggtaatgag tttactgtag
     5101 aagcgaatcc tggtgatttg acccgtgaaa aacttcaagt gatgaaaaac tatggcgtca
     5161 atcgtttatc catgggagtg cagacatttg acaatcgctt attgaaaaaa attggacgta
     5221 aacatactgc agaagatgtg tatcaaacaa tgcaattttt agaagcagaa aattttacca
     5281 atgtaagcat cgatttgatt tatgcattac cgggacaaac actagaagga aatcgggata
     5341 ctttggaaaa agctttggct ttggatctgc ctcactattc tttgtactca ttgatcctcg
     5401 aaaacaaaac aatgttcatg aactgggtac gacaaggaag actcgaactg cctgatcagg
     5461 aaacggaaac cagaatgttt gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc
     5521 aatatgaaat aagtaacttt gggctctccg gacatgaatc tcaacataac ctcatgtact
     5581 ggaacaatga ccactatttt gggtttggag ctggtgctag cggttatctt ggagatattc
     5641 gttaccgcaa caaaggacca atccaacact atttacgccc attacgagca aatgaacgcc
     5701 cggtattgga agaagaaaaa ttgacacgaa aaaatcaaat agaagaagaa atgttcttag
     5761 gactaagaaa aaaaacaggg attactaaaa aacatttcta tgatcgctat caaaaaacaa
     5821 ttgaatcggt gtacggtgat gtgctaaggg aattagagca agaagggtta cttgttaatg
     5881 aagaagaccg tgtttatttg acaccaagag ggactttttt ggggaacaat gtgtttgagc
     5941 gctttttatt tgaaaaatag tttgaagaat atttgttttt tatcgaaact tgaaaagaaa
     6001 aagtttagaa agtttgacaa tgaggtattg aagtattgat ataactggat ttacatcaat
     6061 tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag
     6121 gagaagggct tcttgctata tttataagtg aattagcact tagaatatat gagtgctaat
     6181 gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg atcatccaac
     6241 actatacgag tagtggcgtg cccgttggtt ccaaaacatt aatgtctgag ggagtcaagg
     6301 ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga ttgctgttga
     6361 agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat tacgtggatc
     6421 atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt cagtctctag
     6481 gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata ttatctgagc
     6541 tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga ctgactggct
     6601 ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca gatatgggga
     6661 atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa ctagaaaaaa
     6721 tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg taccataaac
     6781 ttcgtacgga gattccgatg attcttcata aatatttcca gacgacagaa gggatgtctc
     6841 agttatttga caccgttctt ggtcatgttt ttgaagaaaa ggtttatgtc agtggacaaa
     6901 tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg atgttttctt
     6961 ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc aatattcata
     7021 tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg attcaagcaa
     7081 gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct actagtatgc
     7141 cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca gctaaacttg
     7201 ctgattacta ccgctcgctt gacttgtcaa actcgtaatg tgaataacga agctatttgc
     7261 agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa taatgaaagg
     7321 aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca gatgctcagc
     7381 cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa gctgaagcgg
     7441 aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat aaatacttgc
     7501 gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt gagcaattac
     7561 aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac aacctggaac
     7621 gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa
     7681 tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa atccctgcta
     7741 aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca gcgactgaag
     7801 atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta catgatcgcg
     7861 tgttaagacc aactatggtc attgttgcac aataattgtg cggatataaa acttacactt
     7921 tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact taggtacgac
     7981 taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg
     8041 aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag taggggaagt
     8101 tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac gccatatcgg
     8161 tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag aaatctcagc
     8221 aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa
     8281 agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc
     8341 tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg cagctgctct
     8401 tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg accttggtgg
     8461 cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg tattgtcaac
     8521 agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg actacatggt
     8581 agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg ctttacaacg
     8641 cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat
     8701 cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa tgacattgac
     8761 acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag taccagtacg
     8821 tcaagcgtta aaagatgcag gtctgtcaca atcagaaatc gacgaagtta ttttagtcgg
     8881 tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag gaaaagaacc
     8941 aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc aaggtggcgt
     9001 gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt cattaggtat
     9061 cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga ttccaacaag
     9121 taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca tccatgtatt
     9181 acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc aattgacaga
     9241 tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata ttgataaaaa
     9301 cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa aaatcacgat
     9361 caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtgaaag atgcagaagc
     9421 aaacgctgaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg atgtagacgc
     9481 attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg acgaagaaga
     9541 agtgaaaaaa gcagaaacag ctcgtgacga attaaaagcc gctgttgaag caaacaacat
     9601 cgaggaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa
     9661 actatacgaa caagctgcac aacaacaagc tcaagaaaat ccagaagcag ctcaaggcgg
     9721 agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata aataatcagt
     9781 tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt gaaaaatagt
     9841 ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg
     9901 atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg aagtcttagg
     9961 gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa
    10021 ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag aagtatcaga
    10081 agcatatgag attttaagtg acccgcaaaa acgcgctgca tacgaccaat acgggcatgc
    10141 aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg gattcggcgg
    10201 cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct ttggaggagg
    10261 cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat atacggtcga
    10321 cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata atcgggaaga
    10381 aatctgtcat acttgtggtg gaaacggtgc aaaaccaggc acacaaccaa ctacttgtca
    10441 taaatgtcat ggtgcaggta cgatcaatgt agaacgccaa acgccacttg gacgagtgat
    10501 gagccgtcaa acttgtgatg tttgtcatgg cacagggaaa gaaatcaaag aaccttgtcc
    10561 aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga atgtaccagc
    10621 tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg
    10681 cggaccattt ggtgatttgt atgtggtttt tcgtgtagaa gaaagtgata tctttgatcg
    10741 tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag ctttggggga
    10801 tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg ctggaacaca
    10861 gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac
    10921 aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg aacaacaaag
    10981 agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga
    11041 cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt agtttttcta
    11101 gtaaaaatat aaagtagact tgcccaaatt ttctatttct agtaaaaacg ctaagtcatt
    11161 aataattaaa aaccagacga acaatcatac gttcgcctgg t
//
LOCUS       DACDIP010000078        11193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDIP010000078 DACDIP010000000
VERSION     DACDIP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.078"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..652
                     /locus_tag="IV257_002369"
                     /pseudo
     CDS             132..652
                     /locus_tag="IV257_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(554..1270)
                     /locus_tag="IV257_002370"
     CDS             complement(554..1270)
                     /locus_tag="IV257_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8815460.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1320..1529)
                     /locus_tag="IV257_002371"
     CDS             complement(1320..1529)
                     /locus_tag="IV257_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815461.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1806..3079
                     /locus_tag="IV257_002372"
                     /pseudo
     CDS             1806..3079
                     /locus_tag="IV257_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3186..4217)
                     /locus_tag="IV257_002373"
     CDS             complement(3186..4217)
                     /locus_tag="IV257_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815462.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4224..5054)
                     /locus_tag="IV257_002374"
     CDS             complement(4224..5054)
                     /locus_tag="IV257_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8815463.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5051..5857)
                     /locus_tag="IV257_002375"
     CDS             complement(5051..5857)
                     /locus_tag="IV257_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8815464.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5863..6687)
                     /locus_tag="IV257_002376"
     CDS             complement(5863..6687)
                     /locus_tag="IV257_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP8815465.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6677..7777)
                     /locus_tag="IV257_002377"
     CDS             complement(6677..7777)
                     /locus_tag="IV257_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8815466.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7955..8740
                     /locus_tag="IV257_002378"
     CDS             7955..8740
                     /locus_tag="IV257_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP8815467.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8773..9156
                     /locus_tag="IV257_002379"
     CDS             8773..9156
                     /locus_tag="IV257_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8815468.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9232..9363
                     /locus_tag="IV257_002380"
     CDS             9232..9363
                     /locus_tag="IV257_002380"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8815469.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9332..9568
                     /locus_tag="IV257_002381"
     CDS             9332..9568
                     /locus_tag="IV257_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8815470.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9646..10617)
                     /locus_tag="IV257_002382"
     CDS             complement(9646..10617)
                     /locus_tag="IV257_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8815471.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3343 a         2584 c         2135 g         3131 t
ORIGIN      
        1 aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt tcttcgtttc
       61 aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca ccctaccaaa
      121 ggagtcgctc tatggataat catactagaa aattactcgg attaacagat aaacatttat
      181 tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg cagtttatta
      241 aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt cgtattatcc
      301 gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag ttgactattt
      361 tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt tctgcgaaaa
      421 cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg attctcatag
      481 aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc ggctcaagcg
      541 atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac ggcattatca
      601 tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt tgtgcacaat
      661 ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca tcacggggat
      721 ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct tgcggtccgt
      781 aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc agttcttgct
      841 cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct cctgcaatgt
      901 tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg taaagattga
      961 caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac aattgacgtt
     1021 tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta aaggcgaaaa
     1081 aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg gcaccgactg
     1141 gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac aacaaataac
     1201 cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa tcaatttttt
     1261 ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca gaattgagac
     1321 tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc cactggtctc
     1381 ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc aacaccttgt
     1441 tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc ttcagtaaat
     1501 agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca tcaaaatcaa
     1561 aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa gctgtgtcct
     1621 gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt gaaagaaatg
     1681 tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt gagcataaaa
     1741 aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc aaaggagcgt
     1801 tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat ggaaacattc
     1861 aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt gtttctgcca
     1921 cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa catgggctga
     1981 tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg aatcaacgga
     2041 cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc tattttacgg
     2101 cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat tataaaatat
     2161 tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc ggcgtttctt
     2221 ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg aaaataaatt
     2281 ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa gtcggtccct
     2341 attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt ccttcacgta
     2401 agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt ttaaacgtaa
     2461 aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag tgctttcctc
     2521 atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc agttttaagg
     2581 atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa gccaaacact
     2641 atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg cttgtttatg
     2701 ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt gaagtcgtag
     2761 aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat caagaaattc
     2821 ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa acaatgccca
     2881 agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat gaatccggca
     2941 ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg catacccaca
     3001 tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt tcttaatcaa
     3061 tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa ttctacctta
     3121 gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg tttgccgact
     3181 atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat tgctagaata
     3241 tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt caaccgatcc
     3301 actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt gcctaaaata
     3361 aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc aaattgaatc
     3421 agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc gtgggtatac
     3481 tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc aacaaatcgg
     3541 ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg cccagcttcc
     3601 atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt cacaaaaatc
     3661 atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg ttttctaatt
     3721 cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc agtgaccaat
     3781 gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac atttttatct
     3841 gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg aatcacctca
     3901 ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt catagttgga
     3961 aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg cttatcggca
     4021 atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc cgtgatacag
     4081 cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag ctcacccttt
     4141 tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag attgagatca
     4201 tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt cgactgcttc
     4261 tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag cataaaggta
     4321 gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct agccaaatgg
     4381 ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg accgccaatc
     4441 aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag agaaactagg
     4501 gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga catcggggcg
     4561 cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat ctggttgtta
     4621 agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa cactacaaac
     4681 attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat cactcctggt
     4741 aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg gaaatacaaa
     4801 tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac cacacctgct
     4861 aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag ctgttggaac
     4921 cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga gctggcatac
     4981 aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa aatcggtttt
     5041 attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac attgccgtta
     5101 ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg gcgaacacat
     5161 ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg ctggtcaggg
     5221 catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag gtgctggcaa
     5281 tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat gaagcgccca
     5341 gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga tacaaaaaca
     5401 agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac caagaataca
     5461 aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc cccaacaaaa
     5521 taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg gaaacaaagg
     5581 tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa ataaacgtcc
     5641 cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca aaggcctgtg
     5701 tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg ttagttaaat
     5761 cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat gcacagaaga
     5821 taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc tcatcaattc
     5881 ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa acaatggagc
     5941 cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg aaatcaaata
     6001 aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg catccatccc
     6061 gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa ggtaaatagc
     6121 caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg cggtaaattt
     6181 gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct gatactgatt
     6241 cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct cactataaaa
     6301 gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca aacgattata
     6361 gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg tttttccgcc
     6421 agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat ggtaattact
     6481 atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata acggacgtga
     6541 attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct caactaaatg
     6601 attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta gtataactac
     6661 aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa agctctggaa
     6721 gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc gcttcataag
     6781 catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg cgaataggtc
     6841 gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat tcccgtgcta
     6901 gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac ccgctaatga
     6961 tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg tcacgataat
     7021 ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca agatttgcta
     7081 cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag taattcagac
     7141 ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat acagcaaatt
     7201 gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc tcttgcagct
     7261 ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag gaaatggttc
     7321 ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga atttcctccc
     7381 aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga ccgaagctga
     7441 taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac ttagccaatt
     7501 cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt tcaaggtcgt
     7561 tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca atctctcctt
     7621 cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc gattgcttcg
     7681 ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca caaaataaac
     7741 cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc ctttcgataa
     7801 ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt ttcatgaaca
     7861 atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct tctaaagctt
     7921 ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc agcatctcca
     7981 cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct atcagccgga
     8041 gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa aagatgacat
     8101 tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct attccaccaa
     8161 atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga tcgtggatta
     8221 cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg gccgctttga
     8281 ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg aatattttga
     8341 tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc actttgatta
     8401 tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg aggctcagct
     8461 gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa atgccaagag
     8521 cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt atcaaagtgt
     8581 cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt atcagctggg
     8641 ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc tagtcattca
     8701 aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata atagaagaaa
     8761 gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat ccattattta
     8821 attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt gttagcagcc
     8881 gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg ggaaaaagtt
     8941 caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc gcagtttcat
     9001 cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca gctgcaattg
     9061 acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg ccttggtgat
     9121 caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg cgtttcagtt
     9181 gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg tgtgctggtc
     9241 accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct ggctgtagcc
     9301 aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg ctcgtgtcat
     9361 taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag ctgtttccag
     9421 ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta atggcgcctt
     9481 ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg tgcaaaacac
     9541 aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg aggactaccg
     9601 gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga aatcgtagca
     9661 gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt gttcattcga
     9721 caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca aaagctctat
     9781 tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa agcttggatt
     9841 caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa ccgtgcgagc
     9901 cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt ctgccaacgg
     9961 atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg gtagtaattc
    10021 taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca gctcggtcag
    10081 gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt taggatactc
    10141 aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg tccattgttt
    10201 gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat agggagctac
    10261 cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat tgattccaat
    10321 gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg cctcgcctcc
    10381 gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct caatctcatc
    10441 cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct tgctttgtga
    10501 ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa caacggtccg
    10561 taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc cttccatata
    10621 gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt accatttaat
    10681 cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa aatctggtca
    10741 aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct aaatcatttt
    10801 ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta gatatcaaac
    10861 ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt gaataacatt
    10921 caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa aaaatatata
    10981 cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt accttaaatg
    11041 attccattta ctttttatct cattttttcc tactagttag tatcaaatac tctcctattt
    11101 tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt gtcattacag
    11161 caaaaaatca cgttatccag actgtagaca aag
//
LOCUS       DACDIP010000079        11032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDIP010000079 DACDIP010000000
VERSION     DACDIP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.079"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(89..1669)
                     /locus_tag="IV257_002383"
     CDS             complement(89..1669)
                     /locus_tag="IV257_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8815472.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2070..3446)
                     /locus_tag="IV257_002384"
     CDS             complement(2070..3446)
                     /locus_tag="IV257_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8815473.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3574..4692)
                     /locus_tag="IV257_002385"
     CDS             complement(3574..4692)
                     /locus_tag="IV257_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8815474.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4834..5268
                     /locus_tag="IV257_002386"
     CDS             4834..5268
                     /locus_tag="IV257_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8815475.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5346..5879)
                     /locus_tag="IV257_002387"
     CDS             complement(5346..5879)
                     /locus_tag="IV257_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8815476.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(5973..7151)
                     /gene="mutY"
                     /locus_tag="IV257_002388"
     CDS             complement(5973..7151)
                     /gene="mutY"
                     /locus_tag="IV257_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8815477.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7144..7941)
                     /gene="recX"
                     /locus_tag="IV257_002389"
     CDS             complement(7144..7941)
                     /gene="recX"
                     /locus_tag="IV257_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8815478.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8048..9430
                     /gene="rlmD"
                     /locus_tag="IV257_002390"
     CDS             8048..9430
                     /gene="rlmD"
                     /locus_tag="IV257_002390"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8815479.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9604..10182)
                     /locus_tag="IV257_002391"
     CDS             complement(9604..10182)
                     /locus_tag="IV257_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8815480.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10504..10974
                     /locus_tag="IV257_002392"
     CDS             10504..10974
                     /locus_tag="IV257_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8815481.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         3283 a         2218 c         1903 g         3628 t
ORIGIN      
        1 ccgtcacggc tctaaacaaa atatgagagg ctgtttttgt ttggctattc ttattacttg
       61 atgcagggca gcttgtgtca agacttcttt acatcaaact ttttaatttt acattgggat
      121 atttatccgc aaaccagcgt tctgcaaatt ggttctcgaa taagaacaac ggttggtcga
      181 aacggtcacg agccaagata ttgcgacttg aactcatctt ctcatccaaa tcttctggat
      241 cgatccagcg agcgattttg tgtcccattg gagacatgat tacttcggcg ttatactcat
      301 ttaacatacg atattggaat acttcgaatt gtagctgacc aacagctcca atgatatatt
      361 cttccgttag atatgtttta taaagctgaa tcgctccttc ttgaactaat tgatagattc
      421 ctttgtggaa ggatttttgt ttcatgacat tttttgcagt cactttcata aacagctctg
      481 gagtaaatga agggagttct tcataagcaa cttttagttt tccttcgaac aatgtatccc
      541 caatctgata attgcctgta tcatatacac cgatgatatc gcctgctact gctttttcga
      601 cgttttctct cgaatctgcc ataaattgtg tgacattgct taatttcagt tttttatttg
      661 tacgttccaa ccagacatcc attcctcgtt cgaatgttcc agaacagatt cggacaaaag
      721 cgattcggtc acgatgggct ggattcatat ttgcttggat cttgaatacg aaacctgaaa
      781 attcttcctc ataaggactt accatctgat catcttgtgt tttgtgtcca taaggagctg
      841 gtgccaattc aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc aatgcagaac
      901 caaaaaatac tggtgtctga tcgccacgag caattttttc ttgattaaat gcatctccag
      961 cctctttgat tagttctact tcttctaaga cttgttcata taaggaattt tcagttagag
     1021 gatgattttc gggaagcagt ccatcttcat ccaattctac taatcgttca tcctgataat
     1081 tttccggacg ataaaactct acacgattat ggtagatatc ataaagacct tctagccctt
     1141 tacccatacc gattggccaa ttcatcggat aagattcgat atctaatagc tcttctaatt
     1201 cttctaacaa ttccaatggt tcacgaccat cacgatctag tttgttgata aaagtaaata
     1261 tcgggatgcc acgttttttt acaacttgga ataatttctt tgtctgtgct tcgatccctt
     1321 ttgcactatc gattaccatg accgcactat ccacggccat caaagtacga taagtatctt
     1381 cagaaaaatc ttcatgtcct ggtgtatcca agatatttac tcgtttccct tgataatcaa
     1441 actgcataac agaactagta acagaaatcc cacgttgttt ctcgatttcc atccagtctg
     1501 atttggcaaa attacctgtt tttttgcctt ttacggtccc ggcttgacgg atagccccac
     1561 caaacaatag taattgttct gtaatcgttg ttttcccagc atccggatgg gaaatgatcg
     1621 caaaagttct gcgattgtcc acttgttgct ttaattttgg attattcata acctaactcc
     1681 atttcttcca ttcaattatt tatattttct ctaggcacag attgtccgat ctcttaaaga
     1741 aagacaattt catatagggc tgtgacaaaa gccgtgtagc agtctctttt tccgagtaaa
     1801 cggcgttcta gagacagcct atcagcaata agcccaccca agccaaaaat acgaaaagct
     1861 gtttttgctt ggctgctctt attacttaat gcaggatagc tttttcacaa cctttgtcaa
     1921 ctactacaac tgaatcagta tagcatggat tcagcgaatg aaaaagccac caaaccataa
     1981 gagtcaaata tttttttgtc agctgactaa aagaaaagac gtgttgcttg tttattcaag
     2041 caacacgtct ttcataacca agtttccaat tatcttcttg gctcgtcatc ttcgaattct
     2101 tttctgaagt atcttcgttc ttcttcaggt tccgcttctg tttcttccgg atcatgaaaa
     2161 atgacacgga ttttaaggat tcgagaacct tccatttctt cagaaaccag cgtaatatta
     2221 tcgacatcaa acgataactt ttcgccttca tccggaatca tgcctaatgc tgtaatcaaa
     2281 taaccagcca tcgtatctac atcactcata tgcagatcac tgtcaaaagc ttcattgaac
     2341 tcatcaatca gcatacgtcc ttggatgata tattcgtgtt catcgatttt ttcataaaga
     2401 ttctcaactt catctgattc atcatcgatt tctccgacga tttcttctaa aaggtcttct
     2461 agtgttgcta acccaacgac gccgccatat tcatctaata aaatagccat ctgattctgc
     2521 gtctttttca tttcatataa cagatcatca ataaagaccg tttcaggaac gaataaaggc
     2581 tcctgcatga ttttcttgat atctaaatta tcaaagccga atttatgggc agcttttagt
     2641 aaattctttg tatgaaggat tccgactact ttatctttgt cttcgttata aacagggatt
     2701 cttgaatagt tttcggataa aacttcattc acattttctt ccacagtgtc gttgatatca
     2761 atcataaatg catccgttcg tgggaccatg acttctcggg caactttcgt atcaagagaa
     2821 aaaacccctt ggagcatttc taattcttca ttattcaata cgccttcgtt ttcaagcata
     2881 taacgcattt catctctggt catttttgaa tcttcatcat caaaagtcat cggcgtgata
     2941 cgcgccaata aacttgtgga agcagaaagc agccagacga agggcttagc tactacacca
     3001 atcaagcgta tcagccctga agtgaacttt gcgacttctt ctgatttatt taaagcaata
     3061 cgtttagggt acaattctcc aaaaacaatc gatacatagg tcaaaatagc cagcacaatg
     3121 atatttgcaa tattttttgc cgctgtgccg ccacctaata ccggagctaa ccgttccgat
     3181 aaagtattag ctagtgaagc accagacaaa atgttgacca aagtgattcc tacttggatt
     3241 gtcgatagga aattatttgg attctgcaat atttttaaaa gcttttgaga tttcacatct
     3301 ccttcatcag ctttttgctc gattcgattc ttattgatcg aaacaacagc aatttcggaa
     3361 gctgctaaaa aagcattgat caatgtcaaa acgatcaata ataaaatctg cgctaacagc
     3421 gactgactct cagggtcagc attcataatc gcattctctc cttataaaat taatttttag
     3481 tttacgatag aaaaaaacgt ggcactatag ccacgttaaa atggtatcac aaagcaattt
     3541 tctttgcaat aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa ttttcttctt
     3601 gttttcattt tgcttgtctt ctttgacgat tttgattata taggaaatga tcgtcttgca
     3661 gattgcgtaa aaaggaacac ctaagaagat accaagcaat ccggcaatat ttccagctac
     3721 tagaagaacg ataatgatcg ttaatggatg gattttcagt gatttcccaa ttacattagg
     3781 ataaatgata ttcccatcca actgttggac gattagcaca accacacaac aaagtaatgc
     3841 ttttaccgga tcattgaaga ctgttactag aaatgcaggt gctagtccta gataaggtcc
     3901 taaatatgga attaggtttg tcaatcccgc aatgacccca aataagaaag cataatccac
     3961 accaattacc atatatccta aaaatgtgaa ggtaccaacg aataaacatt cgattgcttg
     4021 tccgctaatg taattagcca atgttttatt cagttgcccc aataactcca caatttggtt
     4081 tcttcttttt tctgggaaaa actgttggat gtttggcact agtttttctc cgtctttcaa
     4141 catatagaac aagatgaatg gtgctgtaat gattactacg gttgccgaag cgatcgttcc
     4201 aacgatcgaa cctaaactat tggatactcc actaaggaat tgctgaatga ttgtcccgta
     4261 agaaatgtct aattgctcaa aatatttatt gatatctact tccttgaaca aaggcaattc
     4321 ggctacctct tttaaccagc tttctacctg tttgatgaaa tcaggcatat tagatgccaa
     4381 tgaggaaatt tgctgtacta gacttgggat gacactaagt aaaatccaaa taattgctgc
     4441 aactaaaagg agcagcacaa ttgcgacagc ccatattctt ttgatttttg ctttcatcaa
     4501 cagcttgaca ataggattca ataaatagta aagaaagcct gcaatcagta caggtgcaaa
     4561 taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag tttatttctg atgaaatcat
     4621 gatcaatgca gctatgatca ataattcaag cgaccaaaac attagtttag actgtctaag
     4681 tttttcaaac aatagatttc ctcctttctt tcttactagt atatattata ttcgttaaaa
     4741 attcttttat tcttttttta tttttgaaaa cttgccatac tttttatttg ttttattttc
     4801 cagtatacta tacgaaagga aggtggctag accatgaaag ttgtacaaac aaaagataca
     4861 atgagcaata tttacttaga tgctgtacgg atcaggcatc aagtttttgt agtcgaacag
     4921 ggtgtgcctt tgtctcgaga aatagacaaa gatgaagccc attgtatcca cttcgtcctg
     4981 tactcagata aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga aaacggaaaa
     5041 atgaaattac agcggatggc gattctttct gaatacagac accaagggtt aggaaaaata
     5101 ttgattgaag aagcagaaaa ctttgctaag aatcaaggct ataatacgat tttattaggg
     5161 gcacagtcca cagctgaaac tttttacgaa aaactaggat atacagctta cggcgatcct
     5221 tttgaagatg ctggcatgcc tcatatttat atgaaaaaga ctttataaaa atttatttcg
     5281 aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc actggcttac tgttcttttt
     5341 tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc aaatatcaat atagccttcc
     5401 gagaacgggc catctccatt attgatccag tcaaccagaa tcttcacatt ttcttttagg
     5461 atgaagtcaa tatcgttcgg gtaattcatc attttgctgt gcatctcata ttcatcgaca
     5521 tccaatagtc gtttctcgcc gtcaggaaat accttgatat ccaagtcata gtcgatatat
     5581 ttcaaggcct catcgtccag caaaaaaggt gaagctagat tacaataata ggaaaccccc
     5641 ttttctctga tcattgctat tacattgaac cagtattttt tatgaaaata aacaatcgct
     5701 ggttcgcgcg ttacccatcg gcgtccatca gattcagtga ctaatgtgtg atcattcacg
     5761 ccgatcatag agtgttcgct tgtttttaat accatggtat ctcgccacgt tcgatgcaat
     5821 ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc cttcttttgg aactcccata
     5881 gcaacccttc cttaagcagc attttgtgct ttgtgtttgg gtgttcatta caccgacatc
     5941 ataattatta tatcaataaa aatgagcttt gcctagaaag gattcctatt gtttcgattt
     6001 ttctctaact gatctaccag tttcatttgg accttaggaa aaacgaccga atcaaatgca
     6061 tcaggcttca accacttcga agttttgttc tcggtaaatt cttgttctgc tccctcggtt
     6121 gcccgtccgt aaaaaaggag aacatgccat ttcagatgac taaaaatatg agtaacttct
     6181 cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt caaatgggag attttgctct
     6241 tcttctgcta ctaggtcatc aaacagatcg atttctggct ttgtttccca ttcttttttc
     6301 aatcgctcat actcctcttg tgtcacttcc atcattggaa atgtccacat attcgccagc
     6361 aatttttggc tatctctttc ttcaaaataa tacgctccag aatgattttg aagagctgca
     6421 ctgatataat aaacgtcttt tggctttgct ttttttgttt ttacaggaaa actcgtttgg
     6481 attcctcgtt tgttagctaa acaaaaggct tggatcggac aagtctcaca ctttggagaa
     6541 gttggtgtac agatagctga acctagatcc atcattgctt ggttgaattc acctggatgc
     6601 ttttcatcaa tgattttccg catcgcttca tcaaagattt tcctgctaga agcttttgcg
     6661 atgtctgctt caatgcaaaa caatctgctg actactcgca tcacgttgcc gtcaactgca
     6721 ggttctggaa gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg tcctattccc
     6781 ttcaatgagc taatttcttc aggcgtttgc ggcatttccc catcaaattc tgacatgatc
     6841 tgtttagcag cagcttggat gttccgggct cttgagtaat aaccaaggcc ctcccatgct
     6901 tttaaaagct tttcttctgg agcatttgct agctcttcaa ttgtaggaaa ccattccatg
     6961 aaacgataaa aataatcgat cactgtgtct actctggttt gctgaagcat gatctcagag
     7021 atccagatac gataaggatc acgattatag cgccaaggca ggtttctttt ttcttgttca
     7081 taccattgaa tgaactgatc ttgaaattct tttgtttctt tatctgtcca tttttcccat
     7141 tctttattca ctttcctctt ccttttccgt aatgaatcgc tggatcatat ccaaatcaaa
     7201 tcctttttga tataagcttt gctttacttt tagatttctt tttttaggtt caaaccttaa
     7261 atttcttcgc catagtttat ctccttgttt gactaaataa tcatattcta tttcttgatc
     7321 tgcttcttgc ggaagatcag ctatcgcttg ttggatcact tcttgagaaa agcctttcgt
     7381 catcaatgtt tgttggattt ttctcaataa ttctttttgg ctttttccgt gattttttcc
     7441 aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg tatattggct
     7501 gagcgcttgt tcaatgattt caggtttcaa acctttttgc tgcatctgct gcgctaattt
     7561 ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca taactttccc catagaccaa
     7621 atcatcgaca aggtccaatt cttttaagcg ggctacgatt ttatttcgat cttcttgaag
     7681 gatttccttt tcttttaagt atgttcggat ttctttttcc gatcgcaact gataacttaa
     7741 gtaattcatt gctaggtgaa aaccgaaatc agctccgcta ctcttcttga tctcttggat
     7801 agtttcttcg tctaattccc gtccttttaa taatcgatag cgtactaata catcttcaga
     7861 tacttgtaaa gcttctcctt cagataaatc tacgcgatag aaagggcctt tgcctttact
     7921 gattctcgta acggttaaca taaacatcct cctcgaacat atattctcta tcataccaca
     7981 gaaaatcccc gtaatcaggt attatatgtt agaataaacc acagaaaaaa gaaaaaggga
     8041 gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga aaatcaagcg
     8101 tttaggcata aatggtgaag gaatcggtta tttcaaacgg ttgatcatat ttgttccata
     8161 tgcactacca aaagaagaag tactagtgaa aatcacgaaa gcaactcctc gctatgcaga
     8221 aggagaatta ataaaagtca aaaaaacaag caaagatcgc gtagtggcac catgtcctgt
     8281 ttactatgaa tgcggtggct gtcaattaca acacttagct tatcaagctc aattggattt
     8341 taaaaaggat ctgcttttgc aagcattgga aaaattcaaa ccagcaggct ttcgatctta
     8401 tcaacttttg ccgacgatcg ggatggatga gccttggcat tatagaaaca aagcacaatt
     8461 tcagttacga aaaaataagc agactcaaaa aattgaagct ggtttatacg aagcaaactc
     8521 tcatcagtta gtccctataa cagactgctt ggttcaagaa cctcgaacac aaaaagtaat
     8581 gaatactgtt gttcaactat tgaataaatt tgatgcaccc atctatgatg aacggacaaa
     8641 cagtggaatc ttccgcacaa tcatggttag aatcggtgtt aaaactgggg agttacaagt
     8701 tgtttttatt actcgcagta aaaaattccc gcaaaaaaac gcaatgatcc gtgagatcaa
     8761 tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa aaacgtctct
     8821 cgtgatgggg gatgaaacga tccacttatg gggaaaagaa gccattgaag aacaaatcaa
     8881 tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc agcagaccga
     8941 attgctttat ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa cgatcgtgga
     9001 tgcttattgc ggagttggga cgatcggctt aagtgtagca aaacaagcca aagaagtacg
     9061 gggaatggat accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg aaagattagg
     9121 atatcacaac actcgatatg aagtcgggac agcagagcaa gttctgccta aatggttgaa
     9181 tgaaggtttt cagcctgatg gtatcatcgt agatccacca cgtacgggtt tggataacgc
     9241 attgatccaa tcattattga aataccctcc aaaaaaactt gtttatatct cctgtaatgt
     9301 ttctacactt gccagagatc tggttaattt aagtaaaaag ttcaagattg aatatttaca
     9361 gtccgtcgac atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga tgacgagact
     9421 cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa taagaacagc
     9481 caaacaaaaa tagctcttca tgttttatga atactgtact tgtgaccgaa gagttaacag
     9541 gaggacacta tttatttaaa aaaagggttg tgacatagtt tttgtcacag ccctttttga
     9601 ttattattgg atattcacgt ctatcacttg ataaaatgca tttgtcgtat cgctgatatt
     9661 ccatactgct aaaatcacat tatatccttt tcgatcctgc ggaagtgtga tcgtttggtt
     9721 gactgttttt ggtggaacag ttgctttgtc gtctattttt gtgatcaatt caaaatcact
     9781 aaatttcaaa ggttgattag gattccaagt tggtttggta atgtagtaat cccaagtact
     9841 tgtccgatgt tgtgctgtta aattccaagt aatatttaat gttcctggat taaccatgtt
     9901 tttatgccat ctgttagatg tctgttcatc taaaggctca aagccgctga ctcctgcact
     9961 agcaattttt ccatcaatga atgtattttt ggtagcttcg atactttgcg gttcccattg
    10021 tgctcttccg acattttgat ttaagtttcc tgctgctgat gttcctaggt atgcacggct
    10081 tcctggagaa gtaatataac catgcgcaaa acctggaatg ctaaaaccga acaaccctaa
    10141 actaactaac ccaataaaca ataacgtact aatctttttc atgtagaaac ctccttcatt
    10201 tgataaccac attgtaaacg ctttattgta ttaacacaaa cattatgctt ttataatttt
    10261 atgttgaagt aaaaaaggtg aaaaaagtaa gatgcagatt ataacagaag tagacgagaa
    10321 aagtacaaat agaaaacatg agaatcaaat catatgatct ttttcaaaaa catcattcat
    10381 ataaacaaat gttcaacttt ttaataacaa caattatcaa tttttttctt acttcacaaa
    10441 ctaaatacag gtcttgctgt gagccttcat ggtaaactaa tattatagaa aggagttagt
    10501 aaaatgaaga tgataaaaga tattcgtttt tgggtttgtg ttataggaat tgtcatatta
    10561 ggctttttat cagggttgct tagcggaaat cccggaaaat attactattc tttgcaactt
    10621 ccaccatttg caccacctag ctggatattt gggccgatgt ggaccttact gtatatattg
    10681 atgggtattt ctctttattt gttacttaat cacaacaaca aaaaacagcg aaacaatctt
    10741 gtcggtcttt ttgtgattca gttcattttt aattttatat ggtctgctct attttttaat
    10801 ttaagaaata ttttcattgc agcaatcgat attacacttt tagttatttt cttgtcagtg
    10861 cttatgtatc agttgtggct gcatcatcgc ctggcaatgt ggctcatgat tccttattat
    10921 ttatgggtac tattcgcaac tctattgaat tattcaattt attttttaaa ctaagcgcta
    10981 aaaagagacg tcctgtaatt taacacatag aaaaagtgcc acctaagtta ga
//
LOCUS       DACDIP010000080        11013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDIP010000080 DACDIP010000000
VERSION     DACDIP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.080"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..1458
                     /locus_tag="IV257_002393"
     CDS             4..1458
                     /locus_tag="IV257_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP8815482.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="IV257_002394"
     CDS             1534..2694
                     /locus_tag="IV257_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP8815483.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="IV257_002395"
     CDS             2807..3814
                     /locus_tag="IV257_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8815484.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4864
                     /gene="iolG"
                     /locus_tag="IV257_002396"
                     /pseudo
     CDS             3838..4864
                     /gene="iolG"
                     /locus_tag="IV257_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
     gene            4881..5771
                     /gene="iolE"
                     /locus_tag="IV257_002397"
     CDS             4881..5771
                     /gene="iolE"
                     /locus_tag="IV257_002397"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP8815485.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5794..7389
                     /locus_tag="IV257_002398"
     CDS             5794..7389
                     /locus_tag="IV257_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP8815486.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7405..7719
                     /locus_tag="IV257_002399"
     CDS             7405..7719
                     /locus_tag="IV257_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8815487.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8180..8905
                     /locus_tag="IV257_002400"
     CDS             8180..8905
                     /locus_tag="IV257_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8815488.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            9038..9880
                     /locus_tag="IV257_002401"
     CDS             9038..9880
                     /locus_tag="IV257_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8815489.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            9877..10827
                     /locus_tag="IV257_002402"
     CDS             9877..10827
                     /locus_tag="IV257_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8815490.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            10842..>11013
                     /locus_tag="IV257_002403"
     CDS             10842..>11013
                     /locus_tag="IV257_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8815491.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDI"
BASE COUNT         3475 a         1892 c         2488 g         3158 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgaa gtcgtatcag tatgcgaaga aaatggtaga
     4261 agatggcgaa atcggcgaaa tcacactgat tcgttgttat gggatcgatc cgagcgaagg
     4321 cttggaaagc tttgtcaaat ttgccatgaa taacgacagc ggcggcttgt tcttagatat
     4381 ggcgatccat gatattgatt tggttcgctg gtttaccggc caagaagtcg atcaagtgtg
     4441 ggcgattggg aaaaatgcgg cgtatccaga attggatcag gctggtgagc ttgaaactgg
     4501 agccgcaatg atgagcctga gcgataaaac aatagcttta ctagttgccg gcagaaacgc
     4561 agcccatggc tatcatgtcg aaacagagat catgggaacc aaaggaatgc tgcgggtagc
     4621 gcaagcgcct gagaaaaatc ttgtgacgat catgaacgat caagggatca tccgtccatg
     4681 ttcacaaaac ttcccagaac gattcagaga agccttcatt cgtgaagccc aagaatttgt
     4741 ctcatgtatt caagaaaaac gtcaaccagc ggttaatgca gaagatggat tgcaatcaac
     4801 taagatcgcg ttggcttgtc aagaatcatt cgatacgaag agtttagtac aactaaaaaa
     4861 ataagcagga gtgtgagaat atgaccaaac aaattcaatt agccatcgca ccaatcgcgt
     4921 ggaccaatga tgatatgccc gagcttggta gtgaaaatac ctttgaacaa tgtatcagtg
     4981 aaatggccct tgctgggttc accggaacag agattggcaa caaatatcca aaagatccag
     5041 aggtgttgga acaatatttg aatattcgcg gcttaaaggt tgccagtgca tggttcagtg
     5101 cgtttctgac aactaaacct tttgaagaaa ctgccgaagc cttcaaagca catcgggatt
     5161 tcttacatgc gatgggcgct aaagtcatcg tagtagctga gcagggtcat agtatccaag
     5221 ggattatgac agcgccagtc tttgatgata aaccagtttt cactgatgaa gagtggaaaa
     5281 aactggcgga aggattagaa aaattagggg atttggccca cgaaaaggat atggagatcg
     5341 tttaccatca tcacatgggt accggcgttc aaacgacgga agaaatcgat cgtttaatgg
     5401 aaatgactga tccagacaaa gttaaattat tgttcgatac gggacatttg gtcttttctg
     5461 gtgaaaatcc aatcgcggtt tacaaccgtc atcaagatcg tatcaaacac atccatttca
     5521 aagatattcg tcaagaaacg gctgaagaag tagaaaagga aaaagacagc ttcttaaacg
     5581 gcgtgaagaa aggcgtcttc acggttcctg gtgatgggat gattgatttc actccaatct
     5641 ggacagcaat ccatgccagc gactatgaag gctggatcgt agttgaagca gaacaggacc
     5701 cggctaaagc caatccattt gaatatgcag tcaaagccag aagctacatt cgagaggtca
     5761 ccggtcttta atctattgaa aaggagctag acgatgaatc ttttttctat aatcagtttc
     5821 ctagtgattg tcatctgtgt atggttattt gcctattcta gatccagaag tgtcgatact
     5881 tctggatcag aagggttctt catgggaggg cggagcttaa ctgctttgcc aatcgccggc
     5941 acgatcatca tgacgaacct gtccaccgaa caaatagtcg gacaaaatgg tcaaagttat
     6001 catgcaggga tggaagtaat ggcctgggaa gtaactgcta cgattgcgat cgtagctttg
     6061 gctgtgatat tcttgccaaa atattttaaa tacggcatca atacaatctc agatttcatc
     6121 gagattcgtt atgatacggt taccaaacga attatttcga tcttatttat tgtgacctat
     6181 atgacttctt tcttacccgt ggttctttat tctggatcgc tggtgttcaa taagatcttc
     6241 catattgatg agctgctggg cgtccagccg atcgtcgcga tcataattgt agcgatggca
     6301 attggtattg tcggcatcct ctatctgctg atcggcggct tgtcgttaag tgccaatagt
     6361 gactccattt atggagtcgg tctgctggct tgtggtctgc tggtcccaat tttaggctta
     6421 atgaagcttg gtgacggcag ctttgttggc gggatcgaac acgttgtggt aaacacgcca
     6481 tggctgctta actctgtggg atcgatcgac tcggctgttg ttccatggcc gacactattt
     6541 acagggatgc tgtttaacaa tctgtatttc tggtgtacca atcaaatgat cgtccaaaaa
     6601 gcattgtccg gcaagaattt agctgaggca caaaagggcg ctttcctagt cggcgtcttc
     6661 aaaatcttag gtgcgttatt ccttgtcttt ccagggatcg tcgctcgcaa tatttttggc
     6721 gatagtttaa tgtcaaatcc agataacgcg tatccttttt tagtcagtga agtattgcct
     6781 aaaatgatct ttggtatttt cgccgctgtt atctttggcg cgatcttatc atcatttgcg
     6841 ggagctttga acgcgacatc gaccttgttt tcactggatt tttataaacc gcttatcaat
     6901 aagaaagcaa atgataagca aatcgctcgt gctggtaaaa cagtaacggt cgttgtgggg
     6961 attatctcca ttatcgtagc gccattcatt tcttttgcac cggctggatt gtatcagttc
     7021 gttcaagaat tcaatggact atacaacatg ccgctgctcg tgatcattct ctttgccttt
     7081 tattcaaaaa aagcaactgc ttttggggca aaaacgacga ttgccgtgca tatcgtcttg
     7141 tatgcactat caaaaatgct tttccaaaat atccatttcc tttatgtttt gacggcatta
     7201 ttcgttctgg acattatcat tttgatggtg gtttccaaat tgaaacctga tggagaattt
     7261 gtgttgaact cattcgatac aaaggtcgat atcgagcctt ggaaatacac caaggtgact
     7321 gctatagcat tggtagtcat tgttatttta acttatgtgg cattttcacc aattggcttt
     7381 gcaggctaaa aacaggaggg tattatgcat aaggatctat tgattatttg cgagaccgga
     7441 atcagtgcgt cgctgctcgt atcaaaaatg cttgaaacga tcagagccaa aaatctatcg
     7501 tatgagatag attatgcaac gatccgaaac gttgaagaaa aactgaatta taaagactat
     7561 gacgttttac ttttaacgcc gcaggtcgca cgttctgagg aaaaagtaaa agaaatcatc
     7621 aaaaaggaaa aatgtaccgc tcagatcgtt tatatcaaac aagaagattt tcgttatatg
     7681 aatattgaaa acatcattgc aagcatcgaa gaagattaac gagaatcttt gattattgag
     7741 tgtcgaggag gtggtatatg aaaagatggc atgttcgccc cgatcacccc aatatgaagg
     7801 gcggacttat tagaaatacc caatcttgta tttattcaat agggtaagcg gacctcaata
     7861 gccagctcat ccccaaaaag tcgaatggag acacttaaat gagtgtacct attcgacttt
     7921 ttgagactct aagtcaaatc gtgttgttct cattaaatca ctgaaaggaa atgcttcggc
     7981 gtttcctttt ttctttttgt ttttcttcgt gttttgaaaa ttacttacta tttactatac
     8041 gagtagtttc aaataatttt gacatcctaa tcttcaacaa cagaaaaaaa tctaaattat
     8101 aaaatttagg gttgactaaa atataatttt tgtataccat atgatcgtga taatcattat
     8161 catttagaag gagaaagtta tgttagaagt gaaaaagctt aatgtttgtt ataacgattt
     8221 tttagcattg aacgagattt ctttaaaaat acaagaaggt gctttaacag gtattattgg
     8281 tccaaatggt gctggaaaat ctacattgct taaagcaatg ttaaatatta tccctcatca
     8341 gggagaggtc tccattaata atgaagatat caataaaaaa ctggtgaaga ttgcatatgt
     8401 agagcaaaaa gctgatattg attttacttt tccaataaaa gtaaaagaat gtgtatctat
     8461 gggaacatat gccggaatga aagtttttca aagaattaaa aatgcagaat ggcaaagagt
     8521 ttcaaaggct ttggaaaagg tcgatatggg aaaatattct aatcatcaaa taggagaatt
     8581 atctggagga caatttcaac gtgtactttt agcaagatgt cttgctcaaa atgcggattt
     8641 catattttta gatgaaccat ttgtagggat agacttagtt agtgaacgaa taattatgga
     8701 tacgttaaaa gaattgaaac atcagggaaa aacagtgcta attgtgcatc atgatttaag
     8761 caaggtaaag aagtattttg ataatattat tcttttgaat cgaaatctaa ttgctcacgg
     8821 aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa gcctatggag atactatatt
     8881 tattggagag gggggaacgg cttagcctct caatccaata aatatattat ctcaaaagaa
     8941 atgagttaat accaaattaa aataacgatg gttcataggg ttatccaatc aaaaccggaa
     9001 ggttaaaacc ctaactctaa tatacaaggg gaataatatg cttcaaaatt ttattaatgg
     9061 tctgtatgac tttcactttt tacagaatgc tctaattaca tctgtagtta tagggattgt
     9121 ttctggaaca gtaggttgtt ttattatttt aagaggaatg tctcttatgg gtgatgctat
     9181 ttctcatgct gttttgccag gagtagcaat ttcttatatt ttaggtatta atttttttat
     9241 tggtgcgatt gtatttggtc tattagcttc tatcattatt acttttataa aaagcaatag
     9301 tgtaataaaa ggagatacag caattggtat tactttcagt tcttttttag cattaggagt
     9361 tatccttata ggagttgcaa atagctcaac ggatttattt catatcttat ttggaaatat
     9421 tttagctgta caagatattg ataagtggat tacaattggc gtttctgttt tagtgttggt
     9481 tacagttgtt ctatttttta aagaattatt aatcacttct tttgatccat taatggcaaa
     9541 agcaatgggg atgaatgtca gtttttatca ttatctatta atgattctat taactttagt
     9601 agcagtaaca gctatgcaaa gtgtgggaac tatattaatt gttgcaatgt taattacacc
     9661 agctgctaca gcatatttat atgcaaacag tttaaaaact atgatattta tttcggcgac
     9721 agtcggagcg tgctcatcat tgctaggttt gttcattggt tatagtttta acatagcagc
     9781 aggatcaagt attgttttaa cagcggcatt cttatttgta attagttttc taatctcacc
     9841 aaaacaaaaa tttttaagaa agaaggagag aaagttatga tgagaaaatg gaaagcagta
     9901 ctgggaagtc tggggatttt gattgctctt tttatattcg gggcatgttc aacaaatagt
     9961 aaagacaaag atacagtggc ttcgaacgaa aaattaaagg tagtagttac taattcgatt
    10021 ttagcagata ttactgaaaa tatagcaaaa gataaaattg atttacacag tatcgtacct
    10081 attgggaaag atccccacga atatgaacct ttgcctgaag atgttcaaaa aacttcaaaa
    10141 gcagatttga ttttttataa cggtgttaac ttggaaactg gaggaaatgc ttggtttaca
    10201 aaattagtaa aaaatgcgaa caaagaggaa aacaaagact attttgcagc aagtgatggc
    10261 atagatgtta tttacttaga gggtcagagt gagaaaggga aggaagatcc ccatgcttgg
    10321 ttaaatttag aaaacggtat tatttacgct aaaaatattg aaaaacagtt agcggaaaaa
    10381 gatcctgata ataaaaaatt ctataaagaa aatctagata agtatattga aaaattggat
    10441 tctctagaca aagaagctaa atctaaattt gcttcaattc cgaatgataa aaaaatgatt
    10501 gttacaagtg aaggatgctt taaatatttc tcgaaagcgt ataatgtgcc ttctgcttac
    10561 atttgggaaa tcaacactga agaagaagga acaccagatc aaataaaaca cttagttgaa
    10621 aaattacgca caacaaaagt tccctcctta ttcgtagaaa gcagtgtgga cgatagacca
    10681 atgaaaacag tatcaaaaga taccaatatt cctatctatt caacgatttt tactgattca
    10741 attgcagaaa aaggacaaga tggtgatagt tactatgcga tgatgaaatg gaacctggat
    10801 aaaattgctg aaggcctttc gaaataattt ggagggtagg tatggtacaa attaaaacaa
    10861 ctactatact aatttattta ctagcaattc aggaattatc aaaaagaaaa aagaaattaa
    10921 aaaataatga tttagcaaaa gttctaagtg ttagtccagc atctgtaagt gagatgctga
    10981 ctaaacttag atatgaggac tatttagata tag
//
LOCUS       DACDIP010000081        10971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDIP010000081 DACDIP010000000
VERSION     DACDIP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.081"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            100..369
                     /gene="rpsN"
                     /locus_tag="IV257_002404"
     CDS             100..369
                     /gene="rpsN"
                     /locus_tag="IV257_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8815492.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            384..563
                     /gene="rpmF"
                     /locus_tag="IV257_002405"
     CDS             384..563
                     /gene="rpmF"
                     /locus_tag="IV257_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8815493.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            595..972
                     /locus_tag="IV257_002406"
     CDS             595..972
                     /locus_tag="IV257_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815494.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            993..1142
                     /gene="rpmG"
                     /locus_tag="IV257_002407"
     CDS             993..1142
                     /gene="rpmG"
                     /locus_tag="IV257_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8815495.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1166..2149
                     /locus_tag="IV257_002408"
     CDS             1166..2149
                     /locus_tag="IV257_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8815496.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2179..2301
                     /locus_tag="IV257_002409"
     CDS             2179..2301
                     /locus_tag="IV257_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8815497.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2319..3842
                     /locus_tag="IV257_002410"
     CDS             2319..3842
                     /locus_tag="IV257_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8815498.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            3866..4105
                     /locus_tag="IV257_002411"
     CDS             3866..4105
                     /locus_tag="IV257_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815499.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4298..4933)
                     /locus_tag="IV257_002412"
     CDS             complement(4298..4933)
                     /locus_tag="IV257_002412"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8815500.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4954..5172)
                     /locus_tag="IV257_002413"
     CDS             complement(4954..5172)
                     /locus_tag="IV257_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8815501.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5481..6308
                     /locus_tag="IV257_002414"
     CDS             5481..6308
                     /locus_tag="IV257_002414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP8815502.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6497..6904
                     /locus_tag="IV257_002415"
     CDS             6497..6904
                     /locus_tag="IV257_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8815503.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(7145..8485)
                     /locus_tag="IV257_002416"
     CDS             complement(7145..8485)
                     /locus_tag="IV257_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8815504.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(8761..8916)
                     /locus_tag="IV257_002417"
     CDS             complement(8761..8916)
                     /locus_tag="IV257_002417"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8815505.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9100..9303)
                     /locus_tag="IV257_002418"
     CDS             complement(9100..9303)
                     /locus_tag="IV257_002418"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8815506.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(9213..9545)
                     /locus_tag="IV257_002419"
     CDS             complement(9213..9545)
                     /locus_tag="IV257_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8815507.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10059..10250)
                     /locus_tag="IV257_002420"
     CDS             complement(10059..10250)
                     /locus_tag="IV257_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8815508.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10348..10734)
                     /locus_tag="IV257_002421"
     CDS             complement(10348..10734)
                     /locus_tag="IV257_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8815509.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3640 a         1749 c         2129 g         3453 t
ORIGIN      
        1 tgttgacaag agcaactctt gttggtaaag tatcagtgtc aaaacgtaac gattacgatt
       61 tgagatgtct aaatcaaaat aaaaggagta taaaggtata tggcaaaaaa gtcgaaaatt
      121 gctaaagcaa agaaacaaat ggcgatgatt gaaaagtacg cagataaacg tcaggaatta
      181 aaagccgcag gagatcgcac cgctctcgct aaattgccaa gagattcaaa tccgaatcgt
      241 ctgaggcttc gtgatcagac ggatggtcgt ccaagagggt atatgcgaaa gttcggtatg
      301 tcccgtatta agtttagaga acttgcccat caagggttga ttcctggagt aaaaaaagca
      361 agttggtaaa aggaggaaat atcatggcag taccagcaag aaaaacatct aaggcgaaga
      421 aacggttaag aagaactcat caaactgtag taaaaccaga aatttcattt gacgaagcga
      481 atggtgatta tagacgtagc catcatgtat ctttaaaagg gtactataat ggaaagaaag
      541 taattaaagg agcttcaaaa tagtttaata tataactttt aggagggaat caaaatggaa
      601 attatttacc cacctttagt tgaagaagga ttaaagtatt atcttgaaac gacccagcaa
      661 tcgctagata aatgcacttt ttatcgttcg atggtagaga gaggaattat tactgaaacg
      721 ggtttaccga cccaacaggc aattgaaaat ggattagtca aagattacta tgaggaccaa
      781 ggcttatcat ttgatgattt tttaagaatc tatccaattt ttgaagagta tgacgaggag
      841 ttatttcaat gtattgatgg ttattgggag atacctgttg atatgaaaga aaatttagtt
      901 tcacaattgg aatctggcga gttaactttt gaagatgcac aacaaattca agcatatctt
      961 gaagatcgat aagtattgaa ggaggaagat ttatgcgtca aaatattatt ttggaatgtg
     1021 tcgaaactgg agaacgtctg tatctcacca gcaaaaataa acggaataac cctgaacggt
     1081 tagaattaaa aaagtattcg ccaaaattgc gcagacgggc aattttcaaa gaagtgaaat
     1141 agctaagtgg taaaaaggtg acaaaatggg cataccagtt acaatcgttt ctgggttttt
     1201 aggggctgga aaaacaacgc taatcaatca agccttgcaa gtctcaccct accccaggga
     1261 agaaattatt ataattgaaa atgagtttgg tgaagttggg attgatcaca aattattagt
     1321 tcatagcgag gaacgggtgt tacaactaaa taatggatgt atgtgttgca gtcttcgcag
     1381 tgatttatta gcaatattga tttctttact ggaaacaata gaagaacatc aacaaccaat
     1441 ttcacaaatc atcattgaaa ctacaggtat agcagatccc caaccaatca ttcaaacact
     1501 tttaacagcg cctcaactaa gaggaagagt atatatcgat agtttattga ctgttattga
     1561 tagtgacagt tttgaactta ttgaaagaga accacaagcc cttaagcaac ttgtcatagc
     1621 agatcgcatt tttgtgtcta gaaaacaaaa cttgaattta tctgcactaa aaaaattaca
     1681 gagagaaatc aaaagtatta accctttatc cgatattcga atgttcacac atagtgaaac
     1741 gattcaaaaa aaggattttt ataatttgga aaaattcaat catcccttag tttttgaaag
     1801 tgattcatca gaaaatcact ataatggcaa acactatcat caacatcatc aagaacatga
     1861 gtttaaatca attctcttaa cttcagatca agacataaaa aaagattttt tattccaatt
     1921 aattgaccgg ctgattttaa ccaaccaagg gagcctgtat cgttttaaag gattgattgc
     1981 tttgcaagat caagatttta tagttgccat acaaggggta aacgaacagg ttaattttca
     2041 atacaccact cgacaaaaag accaaaccac tattattcta attggaaaaa tgttggatga
     2101 agaaaggatc aaacagagtt ttgataaatt actggcggaa agcaaataat gataagtatt
     2161 gattaggggt aatttctaat ggagaaaacg gatatatcta gtgcatatcg acgacttaaa
     2221 agcccgaata tcaaaactag aaaaagggca ttaaaaatta tcaaagaaca taaacaaaat
     2281 aaaatgaaga aattagcata attaaggaag gaaaaagaat gaagaaaatc agtgtaggac
     2341 ttattgggat agcagctcta ggattattgg gagcttgctc cagcactaat gatgcaaaag
     2401 tatcgaatga taaagatgga aaattagaaa ttgtaacaac attctatcca atgtatgatt
     2461 tcaccaaaaa catcgtcggc gatgaagcga acgtggattt aatggttcca gcgggatcag
     2521 aacctcatga ctatgagccc tcagctaagg atatggcaaa agctcatgat gctgatgttt
     2581 ttgtatatca taacgaaaac atggaatcat gggtaccaaa agctaaagaa agttggaaga
     2641 aagctgggcc taatgtagta gaaggaacaa aagacatgat tttattgcca ggtagtgaag
     2701 aagaagatca tgatcacgga gaagaagatc accatcatga actagatcct catacgtggg
     2761 tttcacccaa aatggcaatt aaggaagttt caaatatcaa ggatcagtta gttaaattgt
     2821 atccgaaaaa agcaaaagta tttgagacaa atgctgaaaa gtatttgact aaactgaaac
     2881 ggttggatgc tgactatacc actagtctta aagaagcgaa acaaaaaagt tttgtgacac
     2941 aacatgcagc ctttggatat ttagctttgg attatggatt gatacaagtt cctattgctg
     3001 gactaagtcc tgaagaagag ccatcttctg gtcgattagc tgaattaaaa gagtatgtta
     3061 aaaaaaataa gatcaattat atttactttg aaaagaatgc taatgataaa atcgcaaaaa
     3121 ctttggccaa tgaagctggg ataaaattgg aagtattgaa tcctcttgaa agtcttacaa
     3181 aagaacaaat ggataatggt gaagattatg tttcagtaat ggaagacaat ctaaaagcgt
     3241 tagagaaaac aactatggtt gctggtgaag aggtagttcc agaaaaagaa gcgaaggatg
     3301 aaaagacagt tgctaatggg tactttaaag atgccgatgt gaaggatcgc gaattgagtg
     3361 attatacagg cgaatggcaa tctgtttatc cgttgttaaa agatggaatt ttggatgaag
     3421 ttttcgatta caaagcaaaa ctcaataaag acatgacagc tgccgagtac aaagattatt
     3481 atactactgg ctataagaca gatattgata ctatcaacat taaagacaac accattgatt
     3541 ttgtagtcaa cggtgaacat catcaataca catacaaata tgttggatac aaaattttga
     3601 attatgaaaa aggaaaccgt ggtgttcgtt ttaattttga aactgatgat gcaggtgctg
     3661 gtcgttttaa atatattcaa tttagcgatc atggaattgc accaagtaag gcagagcatt
     3721 tccatatttt ctttggtgga gaaagtcaag aaaaacttta taatgaaatg cataattggc
     3781 ctactttcta tccagcttca ttatctgagc atgagattgc tcaagaaatg atggcccatt
     3841 aaaatccaat ctttggagga aatacatggt aagaaaagta ttattgtcta tggattccaa
     3901 gaatattgat gcttatcggc gttatcaatt tgcaaagcaa atgagattaa atgggagtac
     3961 attagctgat attgctgcaa ctttacatgt tagtattgac acagttcata gagtattaaa
     4021 acgggatcct gattgttgtt ttatcaaggt caatactgga agaagtagac gtttagcaaa
     4081 acggcataat agcttggcca attaattgat aataccagag tagatcattt ttcccttatt
     4141 tggatttgat ctgcttttta ttatttcttg aattgtcaaa tcaagcgcaa cactttatca
     4201 aagaagactt cataaggtgt tctccaatct aaacactttc taggtctgtt attcagtttt
     4261 tcaatcatct gatttatcgt tgagttatct accgtagcta tctctttcga ttttggcaag
     4321 tattctcgaa tgagtctatt cgtattttaa tttgttcatt gttgccaagg gcatgaggat
     4381 ctggaaaata aaagggcata ttattcaata cttctgtaac tgcgaaatga ttggagaatt
     4441 cttttcctct atctggagtg atcgttttga ccgcctcatt tggaatactg cttaaaagtt
     4501 caatcatttt ctcgctcaca taagcggctt tctttctggg gaccttcttt cctgctaaaa
     4561 ggaagcatga ttttctgtct gtcaaagtca ctaaacaagt accactagtt ttgccagcta
     4621 caatatcggc tttccaatga ccaaaatcac ttctatcatc tgcggatttt ggatggccat
     4681 ggatcgaatg agtaaggcaa atcttcccac gagactcgac atgatttttt gtatgtcttg
     4741 tttttcctcg atggtgcaat gaacggatca ggcctttatt gccgtgtgag aggggttctg
     4801 tatcgaaaag atcattgcaa attgcacggt agatcgtgat aaaactaatt gaaaagtcca
     4861 tatgttcatg cttcaaacga ttcgagattt tctctggcga ccactgaaaa tcgacaaata
     4921 aatgacgaac aattgaccaa actcacggat catttagaat gtgataacgt ccgcattttt
     4981 ttcttcgata agcataattt ttttccgctt tggaaggtga ttagtttttt tcttgtttct
     5041 tctcagttca tgtaaaatag taaacgggct atgctgaatg tcgttgccaa ttatactaat
     5101 agaaaatcct tcttcgtgaa gcagaaagat tctttctcgt ttgagtatag taagatgaca
     5161 atactggctc ataattattt tccttctagg agtgttctcg acaaacttat actagttgga
     5221 tatcgattat gactcatttt tatgtgttgc acttaaagtg taaattcaag gtatagaaag
     5281 aggttcccta tgaaaaaata cctattacaa tcttaactgg ctatcttggt tcaggaaaaa
     5341 cgatacttct aaatcatatt ctgtacggtg atcacggaaa ggaaattgct gtcattgtaa
     5401 atgacatagg ggaagtaaat gttgatgggg tattagtcga caagaaaggt tttaaaagaa
     5461 ctggagaaaa acttgtttcg atgccgaatg gatgcatttg ttgtacatta cgcgaagatt
     5521 tgattgttga actagatcaa ttagtgcaat tttctgactt gaatcaaatt gttattgaag
     5581 cttcaggaat cagcaaacca attccaatta ttcaagcaat tatattagct aaaaaccctt
     5641 tagacattga tttgacccaa cgattagaga ttggtgccat tattatagta gtggatgtaa
     5701 aacgtttttg ggataatttc gaaacgggtg aaaagattca ggaaagaaaa gctgaaggta
     5761 atgtagttga agaagaacag gatattcgtg gcttgttaat tgatcaattc gagtgttgta
     5821 acatattact tttaaataat agtgacttag tgtcaaccaa gacgatttac aaaattaacg
     5881 gtctaattgc taagttacag tccaatgcca aaataattag gacaattaaa ggtgaagtag
     5941 acttatcgaa aattctaaat acaaatcttt ttaatataga tgagacttcc tattcaatgg
     6001 gctggttcaa agagttagag cttggctatc aaaatcatat tcctgaaaca gaagaatatg
     6061 gtatctcaag ttttatctat cgaaatcggt tactccttga cgcacaaaaa tttgcccatc
     6121 ttacggataa aaattttcct ggcaatatta caagagcaaa agggtttctt tggtttgccc
     6181 aacatagtga tttttcgacg atctttgaaa catcgggtag ccataaaagt attgaatcat
     6241 taaattattg ggtagctagt ttacctaaaa ttgagtgtca aatgattata caaaaaaacc
     6301 cgaattgaag gaagcctggc atccgttgtt tggtgaacgc attaataaac tagtttttat
     6361 tggtatgaag atggatcaac aatgtattaa ggaacaactt gagggttgtt taactgattc
     6421 tcttaatgca gataatttcc gaaaaatttc accatttcca tggaaaaatt ctaatatgtt
     6481 tagccagata tcgttaatga aatttttaag aaaagctggt gtcaagatag gcgatgtcat
     6541 aattattggc attttgatta ttccctcttt tattccctta tttgtattct ctatagaaaa
     6601 ggagaatgaa acaactgaag atgctatcaa atatgccatc gttcaaattg atggcaagga
     6661 aatggatcgc ttcgatttgg ataagatcaa tcataaactc gtgaattatc atcctgcgaa
     6721 gggacaatat aatattgttg agattaaaga tggaagaatt cgagttaaag aagataatag
     6781 tcctgatcaa atcgcggtta gaactggatg gatctcaaaa cccggtcaaa cgagtatttg
     6841 tttaccccat aagttagtta ttagtattga gaaaaaagaa agcaaagatt actacattta
     6901 ctaatctgga aatagatgag gaggttttta ataagaaaaa gatattatta ttaataatag
     6961 caggggcttt attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg gtgacaacag
     7021 gcacaagtat ctttaacggg acctgcaatg gtttagttaa cagtcggtta gattacttat
     7081 tttggctctt cgtcaatgag tgttggtgaa gaaattcaat agaataatcg agatactcac
     7141 acggctagac agctatcttg tctagccgtg ttgttgttct ggctttagtc agttgggtag
     7201 tgttcttagt tttcttgaaa tattttcctc gatataaaaa gattcgttct cggtagtttt
     7261 gaaagtttct aaagccatag gcaattcgtt ttagtacttt gatatggttg ttcaaacatt
     7321 ctatagggcc gttggaatag ggaactcgca acgctcgttt gatttctgtt cgatatttct
     7381 tgaaggtcgt gaacactggt tggtagtgct ctggcaactc cttgtagtct tcttttagga
     7441 gcgcgacgaa ttcaggaaca tcttgtcttc tgatagcgga aagaaaaacc tggtagactt
     7501 cgtagcctcg ctttaaggag tcatcataat ataatagccg atcgactatc tccgcttctg
     7561 tcagatgatc tcgaaatgac ggacgccagt agcgatgctg accattcaat tcccacgcat
     7621 tcttttgaag tagtctccag tatttcttta agtgcttcga ccgacgatcc ccttttttaa
     7681 aggtcttcat tacttgaatt cggtggttgg taaaggcccg accgatgtgt tggctaatat
     7741 gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc tttcaccaat gtgcgatagg
     7801 gtgtatagaa atctgaaacc actagacgta cttgttcacg gacttttcta gggtagcgca
     7861 agaaatagtc gcgtaaagga ttgagttgcc gattttccac aatatcaatc agttgatggg
     7921 tcttgccgtc catcatgaca aagctcatgg ctcccgagac gtttttcacc gatttgaatt
     7981 catcgaaaca caccacttct ggtaacggct ggttcaaaga aacggtttta ggccgaaggc
     8041 tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt catacgtgca atcaaagaca
     8101 tagaaatagg ctcacttaat agctcaagaa tggcttgttt gactctgcgg gcaatggagc
     8161 agcgacgatc gctaactgaa tcttctgcta aaaaagtctt tccacagtgt ttacatttga
     8221 agcgctgttt gttaatccgc acaatggtct tgtagttact gacatcattc aataagatgg
     8281 atactttttt ccagccccat ttaatgatcg tttgcttatt ttgatagtgg caatggaagc
     8341 attcctctgg ttgataggtc aatgtgccat caaaaacaaa acatgtttcg tcattaatgc
     8401 gctctttgcg aaaggcatct tcatgaaagg taagatttag gtctagaata tctagtaact
     8461 ctttggtaaa ctctgaatag gacatcttga aacactcctt tgaatggttt tcgtcgactt
     8521 tattctaaag gatttcaagg tgtctgtttg cgtttattag aaaaaagaaa aagtattggc
     8581 aacagaaggc gtcaccaaca ctagatatta tagatccctt attttcttcg cttcaaattg
     8641 tcaaggagtc atcataaatt ttgtatggat tcttttgttt gggtagtagt cttctaaata
     8701 atcttggatt aacaccttca tctctatttt acttttatat ttgttggagt cgattaattc
     8761 tcatttgatt gatcgatgaa acgcttcaat tactgcattg tcaacaggag tcccagtttg
     8821 gctcattgaa tgtgtgatgt ggtggtcggt caacagctct tgataaataa aagaccgatg
     8881 ctggctacct ctatcggaat gaattaccgt aggcaattct ccttaataaa tttgtaatgg
     8941 acggacgaca atctcttttt tcatgtgtga atccaccata tagtaacgta gaatacgagt
     9001 tgctaagtca atataggtgg acaaatagag gcgaccgtca ttacatggaa tgtaagtaat
     9061 atccgtcacc caaacagcat caatttggtt ctgttcaaat tatcgattca acattttttt
     9121 taccatttct ttgattgttt tttctctacc atgagaagat ttgcgtcgat gaaatcctct
     9181 tgtgcataaa ttcatttcac gcataagaac agctacaaca cgtttattga tttcgattcc
     9241 ttcatcgtaa agtttttgag tgatccctgg agatccgtat cgatctttgt gttcgtagaa
     9301 cactcgtctt cctcttttag tcgtttattt tcctgttcaa gaatgtagaa atctgcttta
     9361 tcaggtaatc tttttccttg tccagcaaaa gcatttttcc cgtatcgacg atattctgaa
     9421 acccatcggg aaattgttgg acgagagata ttgtattctt cagcaatttt cttggtagtt
     9481 acttttttct caaggatatc ctgtgaaatc tttagtttaa atttacgatc atatgttttt
     9541 ttcaatagag ttcattcctt ccggtaacgt aactgatagt caagaaaagt gtatcatagc
     9601 ccaacttagt atcagggttt gtcaactaaa ctgtggaagt tgatagataa gagtttttaa
     9661 caaccaaaat tctcttgact atttttgaaa tcagacaaat tttgattgga cacatagttg
     9721 aataaatcta ccgtcgttac ggaaggtaaa tcgcatgctt ttcaattcta gaggagaagt
     9781 cctcgttgaa gtaccatggc ccagttctgg attcgacctc ctgcccactt cgtacgtaaa
     9841 taaagtaatt ttaggagtga gactgtgaca aaagttattt ttaagctaaa aacgaattaa
     9901 aaaacgttaa taattaaaat cattcgtttt agattctatg aatcattata gaacgctgta
     9961 aaatcgatac tttataatta taaagaagat ggcttcaaat ttattgttcg aaatttgaaa
    10021 ctatcttttt acttatgtca cagactccct tagttgagtt attctttaat ttcgaatttc
    10081 gagatagcta gatatattgc aagcatacat tcttgataac aatcttgatc atattttcca
    10141 gttctccagg attgcttatg gaccaaagcg ctaaatcttt tgagaatggc cacaactgct
    10201 tcttcatttc cattctttgc taaaatgaaa tcttctttta aagtattcat aaatttacct
    10261 tcttcccatg aacttataat ctatttattt ttttgggaag gaggaggacg agcaaagaag
    10321 catacaaaat tagttattat gcagatatta aaccagcatt ttttgaattt ttttgtaaag
    10381 tcgatgtttt aaatttgtcg caccctgtct tgtaatcccc agaacctttc caatttcttt
    10441 atcagtttta ttaaagataa atttttcgat aagaaaaatt tgttcaggtt ctttcaacaa
    10501 aggaaggatt tttgctaatt ttatatcttc aacaaggaga gtcacattta aaacattaat
    10561 cggttgttta aattttatag tatctttagt aaacaaatat ggtgggagtt gatcaagttg
    10621 ttcatgtttt tgaatttctt cttgtttttt ataataagta ttagcggtgt tcaaaatgac
    10681 cttttcaaag aatcgaatca actgggcatt tacgcgttcg tcgttatctg acatattgca
    10741 cacctcctcc attaagattt aaagggaaaa gaggcaaaaa acacgaaaag aagctaatgc
    10801 aattatgctg cgctgcgaat aacttctata agctcacatg cattgatagg actgctgatg
    10861 tttattttca aaaatgtttt tttagttgat aatggatagt tatctttata cttagaaaag
    10921 aaaattcata tatcttctta aatatcaaac atgtccttat ggctaagaaa a
//
LOCUS       DACDIP010000082        10575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDIP010000082 DACDIP010000000
VERSION     DACDIP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.082"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(48..1244)
                     /locus_tag="IV257_002422"
     CDS             complement(48..1244)
                     /locus_tag="IV257_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8815510.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(1368..2030)
                     /locus_tag="IV257_002423"
     CDS             complement(1368..2030)
                     /locus_tag="IV257_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8815511.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            2258..2821
                     /locus_tag="IV257_002424"
     CDS             2258..2821
                     /locus_tag="IV257_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8815512.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2834..3118
                     /locus_tag="IV257_002425"
     CDS             2834..3118
                     /locus_tag="IV257_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815513.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            3137..3829
                     /locus_tag="IV257_002426"
     CDS             3137..3829
                     /locus_tag="IV257_002426"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8815514.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            3841..4386
                     /locus_tag="IV257_002427"
     CDS             3841..4386
                     /locus_tag="IV257_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8815515.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            4657..7935
                     /locus_tag="IV257_002428"
     CDS             4657..7935
                     /locus_tag="IV257_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8815516.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8476..10383)
                     /locus_tag="IV257_002429"
     CDS             complement(8476..10383)
                     /locus_tag="IV257_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8815517.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
BASE COUNT         3287 a         1960 c         2175 g         3153 t
ORIGIN      
        1 aataaactgt tgttgggcgt ttgttttagt aacaagcgaa aggtgatcta ttgggttaaa
       61 tccaacagtt tttgtttcag atcttcttcc atgtgtccaa gctctacttc tgcatgacga
      121 cgtttttctt ttccttcttt ttggatacgc aatgtctctt ggattgtttc gatcaaatca
      181 ttttgagtgg tttgaagggt ctcgatatcc acgattccac gttcattttc tttcgctgtt
      241 tcgatggctg agattttcag catttctgaa tttttcttca ataagtcgtt tgttgtttca
      301 gagacttggc gttgagcagt tactgcatct ttttgacgaa gcaaggtcaa tgcgatcacg
      361 acttggtttt tccacagcgg aatggctgta gcgatagatg cttggatctt ttcagccaag
      421 gcctgatttg tgttttggat caaacggatt tgtggtgctt gctggatcgt gatctgtcta
      481 gctaagcgaa gatcatgcgt acgtttatcc agacgatcta ggaattgcgt gtaatcattg
      541 gcaatctgaa catccatctg atcgccagtt tcttctgctc ttttcatcgc ttctggaatg
      601 atcgttgttt gaagttcttc cattttcaat tctccagcag cgatataaat attcagtgca
      661 tcaaagtagt ctttgttttt ctggtaaagc tgttctaaca tcagattatc ttttagcaga
      721 ccgtcttttt ctttatctaa tttcactgaa atcttgtcaa tttgtgcacc aatcttttga
      781 tattttgctg tcacttcata gatcgattgt ttgaccttcc cgaagacacg ctggaagatg
      841 ttcccttctc cggcacgcag ttcgtcagga ttggcttctt ggagacgata catcagttca
      901 gttaaggaat ctcctacagg accaatatct tgtgcttgca catgattcaa catcgattgt
      961 gagaattcac ttaattttgc ttgggcagcc gatccgtagc tgatgacaga ctgtgcatca
     1021 ttagcatcga tttttgctgc tagctgttta gcttgttctt ggcgttccgc cggtagtttg
     1081 tcaattaaac gagcagctgt ctgttgctct tggagctgat caatctccgc ttgttgtgaa
     1141 gtggtcagcg tatctgttgg agttgaaaac gggttgttca ataaatcttc aagtgtatcg
     1201 ttaactggtg taacatcttt tgtttcagtc ggtttatgat ccataaaaaa ttcctccttg
     1261 ttgaatctgt tctcgattct tacatgaatg tctctacttt agaaaaagta ctcgaaacag
     1321 ccagacatca tctgaatgtt ccgagtcttc ttttatgaac aaaagagtta atccttttgt
     1381 tctggttcat atttattatc acgctttaag ctctgtttcg caacagatat ctctacgtcg
     1441 atatcatcta gatcctcagc aacgaatttt tgataatctt gtgcgattaa agctgacagc
     1501 tggtcgatga tctgcgcgct ttcttctagt ttttcatagg tctgtttgtt tttgatctca
     1561 tggtcattga tctcattata cttatttgtc aaatcaacta gattcggcag atgcgtataa
     1621 agaaactgac ttgcttcatg cagtcggttt ggttctttca ccaattcttt gaataaagct
     1681 ttcgctgctt tgactggatc atggcggaga tcgattgctt taagtttggc tgtctgctgc
     1741 atattcattt gcaattgatt gatctcattt tttgtttgat tcattgtttg tcgaaaaaat
     1801 gaaatttcat tcgctgtcat accgcttttt tcatagtgag tttcaagttc gtcagtcagt
     1861 gcaggaattt cttcttgttt tccctttttc ctgcgcgaac caaagagtcc ccacaaaact
     1921 aaaactacac ctatcaataa aagtcctaga acaaaagaaa aactattgtt tcggacaaag
     1981 agaaatagca agaccaatgc tagaagccac ggccaatttc gttttatcat aataaaccct
     2041 ccatgtcatt tatcgttgag tattgaattc ttttcgttat tcgattacat acttatttta
     2101 gcatacggac tactataaag agtatcggac taaagaagga ttttctatat ttagagaaaa
     2161 tctaaggttt atctatggta aactaataag gagtttttga gaaaaattga aaaatgaaaa
     2221 aaaagcagtt atgattacaa agaaagcagg agaagatatg ctgaacaatg agcaatttga
     2281 agaaaaaaca atcagtcgca aagaaatcta tagcggaaaa attattgatg ttgctgtaga
     2341 cgaagttcgt ttgccagatg gaggaacttc gaagagagaa ctggtatttc atcctggcgg
     2401 tgtcgggatc attgcttttg acgaacaaga tcgtctgctt cttgtcaaac aatttcgaaa
     2461 acctttagaa aaagtgattt tggagattcc agctggaaaa atcgatccag gagaaggaca
     2521 aaatcccgaa atgacagcag ctagagaatt agaagaagaa actggctatc gtgcgaagtc
     2581 gctgagccat cttacttcga tgtatctttc accaggtttt gcaaatgaag tccttcatat
     2641 ttatcatgca caaggggtag agaaggtcga gaatccttta gcacaagatg aagacgaagt
     2701 actggagttg tatcatctaa ctcttgaaga ggcgcaacaa gcgatgaaag atcagttgat
     2761 ttgtgatgca aagacgattt atgcgattca atactgggaa ctattgacca aaggaaagta
     2821 gagggatcgg cttatggcca aaggaccgct tattacgcga agcgaacttc gcaagcgaca
     2881 acaagcacaa gcaagcgagt cattaaaaaa acagcgaaaa gcagaaacag cttatcagca
     2941 agaagaaaaa aagattgcca gcttttatcg taaagaaagc aaaaagaata aaccaatcac
     3001 gaaaacaagg atcagcgaac gggaaaagac gaccaaatgg aactcttttt tgatgaaatc
     3061 tcttattatt gttatcctaa tgttatgtgt ggttttttta gcaatcgcat ttatatagaa
     3121 agaaggaatt aaaaagatga aaatcggtat tatcggcgca atggaagaag aagtaaaaat
     3181 tttgagagag aacctaagcg aaccattatc atgggaacgc gcaggcgctt tatttatttc
     3241 aggttcatta ggtaaccacg aagtgatcgt cgtacgttcc ggtataggaa aagtcttagc
     3301 atctattacc acaagtctgt tgatccaaca atacggcgtg aacatggtga tcaatacagg
     3361 ttcagctggc ggaatcggtg aaggcttaca agtaggtgac atcgttatat cagacaaagt
     3421 agcttatttt gatgcagatg caacaggatt tggctacaac ccaggacaat taccaggaat
     3481 gcctctatat tatgaagcaa gcacatattt gcgcagtgaa atggttaaag cagcaaaagc
     3541 aacgaatttg aacgcaaaag aaggattgat cgttacaggt gatacatttg tcgactcgcc
     3601 agataaaatc aaagaaatct taacgaattt ccctgaagca ttagcttgcg aaatggaagg
     3661 agccgctgtt ggacaaacag ctcgtcagtt caatatccca tttttgatcg tacgtgcaat
     3721 gagcgacaca gcagatcact cggcgacaca aagctttgac gaatttatcg aagacgcagg
     3781 caaacgatca gcagaaatgg tgatcgaatt tgtcaaacat ctcgtataag gaggactaag
     3841 atgaacgcat tgatctcgat tgactacaca tatgattttg ttgcagagga tggaaagctg
     3901 actactggga cagcaggaca agtaattgaa aaaaagctga ctgctcatac aaagaagttt
     3961 attgatcaaa aggattttgt cgtttttgcc attgatgcgc atgatccaaa agattccttt
     4021 catccggaga accgcttatt tcctccgcat aatgtgatag gaacgggtgg acgggaattg
     4081 tacggctctt taaaaagttt ataccaagac tatgagcaag aggaaaatgt ttattggata
     4141 gacaaacggc attattccgc ttttagtgga acagatctag acatccgttt gcgagaacgc
     4201 cagatcacag atatttattt gaccggcgta tgtacagata tctgtgtgtt gcatacagca
     4261 gtcgatgcgt ataatctagg atacaaactc catattttca aagatgccgt ggcaagtttc
     4321 gatcccgttg gacatgaatg ggcattgaga cattttgagt ccacactagg cgcagaaatt
     4381 ttataaaaaa acaaagtgat ttgataaaat aaaaagcaca aaataataaa agaagagcat
     4441 aaaacaaacg actttgtttt atgctctttt ttatacgtta ttttaaaaaa acttattctt
     4501 tttacgttaa taaatctctt tattatcaac aacttaagtt atattttttc tttttataaa
     4561 atacacttat tataattttg ttttttattg acagtaaaaa taaattaggt aatactttaa
     4621 gtaaacgctt tctaatttgt tacgggagga atacacttgt taaaaaaacg tataaaatac
     4681 agtgcgctaa tgatgaatct ccttatgctc agtaccccgg ttttaatttc tgtcgttgaa
     4741 gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg cacacgtgaa cattgaaggg
     4801 atgtcaacaa tggaggaaat accaagaact agtgaagata tggatacgga agaaggaaat
     4861 gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa agcaatctga agaagcaaat
     4921 gaagagtgga aaggggttgt tttcggagaa agcacaagtg ctagtaggta tgccattgaa
     4981 ccattagaag acggcgtgcg gctctcttcc acgaataacg gcggaaaatt ccagtcaagc
     5041 ggatcagatg gaatgaccta ttattatcaa gcaatcccca aagacatcaa ctttaggatg
     5101 cgggcgacga tcgaaataga atcttggacc tatacgaatg cccaagaagg atttgcttta
     5161 atggtaagag acgcagtacc aaaaaatcat ttgtttgggc aggcatttta ttcaaattca
     5221 tttgccatac taggcagtcg gatcgaatat gtctgggact cggatagaca agaagtggtc
     5281 aacacgagcg gcagcaaata tacgatgcga cttggattgg ggacacgggc aatcacagga
     5341 atatcatctg aagatccgca agctgcgcca gctcctggga gtgtctcagt ggaaacaaca
     5401 cctttagaga cttcagcagg gttcttggaa aaagaaacag gttcttataa tatattcgga
     5461 aacggtcatg ggaatctgtt tccggccacg aactcgatta ctaaactgga cgtagagatg
     5521 aaaaaaacga atactggttt agaggcatat tattacgatc ctgaaacagg agaagaaatg
     5581 gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt cagatgaatc ggttatttat
     5641 gcaggatttg ctgcagcaag gaatatgacg atcaaggtag aaggaatcga agttgcttac
     5701 tcagatcctg cgacagatcc tcctgggcaa gaaagaccta ctcgcttgat tgatccgatt
     5761 tacactgtga cctctagtaa tcattcagga aaccaagatc atactatgtc ctttagagca
     5821 aatgctgacg gattattgac aattaaaaat aaagcaacag gagaggtgct taagaacgca
     5881 aaaaacttgg ctatcactgc gaatcgggaa tttgattatc agacaaaact gatggaagga
     5941 acgaacgaat ttactttcag ttttacgcca gataaaaact atccgccaga agaatacgta
     6001 gagatgacta gttatgaaac aaaagagatt cttcatacag tggactaccg taaacctaaa
     6061 tattcgacag tttatgtaac accagaaggg tcagatgcag gaaacggaag cagacaaaat
     6121 ccattatctc tgacacaagc attgagatac gcatatgctg ggcagacaat cgtcatggcg
     6181 cctggaactt attccttcga aagaggattg acgattccaa aaggcgttaa cgggacaaga
     6241 gaaaacccga ttcagttgat tgcagagaaa aatccagaaa acaagcgtcc tgtacttgat
     6301 tttaaaggaa ctggaaatgg gatgacactg ttcagtcact actggggttt acgagggttt
     6361 gacattacaa atagtgcagc tatgcaaaaa ggattacaga tttctggaaa tcataatttg
     6421 atcgaagaga ttcatgctta tcggaatgga aatacaggaa tccaaatcag cggtagctca
     6481 aatgatcctt tcgaagcatg gcccgcgcat aacttagtca agaactgtac atcttttgaa
     6541 aatgccgatc caggatttga agatgccgat ggatttgcgg ccaaactgac aataggagaa
     6601 ggaaatgtat ttgacggttg tatcgcgtat cataatgcag atgacggttg ggaccttttt
     6661 gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca aaaactcagt tgcttatcga
     6721 aacggctggg taccaggaat tgagggagaa ggtaacggta atggattcaa aatgggcggt
     6781 tcttctttaa cgggccctca tgaattgatc aatagtttgt ctttcgaaaa tcttgcgaaa
     6841 ggaatcgatt ccaatagcgg aacagatatt atcgtcaata gcagtacaag ttttaataac
     6901 ggttcatata atgtggctct ttatacctct tcagcaggaa acacagacta ccatgcttct
     6961 ggtatcttat ctttccgaac agagaatctt gagatgagag agcagatccg cccattgaat
     7021 caaaacgaag atcaaatcta ccactcgttg aactactatt ggaatgaacg agagaaacaa
     7081 tcagaaaata ctcttggaca taccatatca aaagattgga tcgagtctct aaatacatgt
     7141 tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg gaagtatcca tgttcatgga
     7201 ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg aaagagtgga tggtcttcca
     7261 ttatctgtag gtgctatttt tgacggagaa acctcaccgt cttcggaata tccagagtat
     7321 gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac aaaataatcc gattattata
     7381 caagaagcaa atggtgtggc ggagaatgca gaaggaaata tcattgaact gaagcctttt
     7441 acagtttttg tgtcaacttt cacaccaaat caaggaacgc ctgtctttga agatatgcgt
     7501 tatttcccag aaaagatcca aatcgttgaa acacagtcaa gtgctcaatt ttctaatcat
     7561 accgctattt tttcagcacc ttcaaaagga gaatatacgc tagaagtcac ttatcgttcg
     7621 gaaatatttg atggggaaaa atggtctgct catacagaat ctgttaccca gcaaaaagcg
     7681 atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg acaaaccaga aagagaaaat
     7741 ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac cttctcatcc ggataggaca
     7801 gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa aaacgggaga atttaataac
     7861 ccgttgctag cattagctgg aggaattttg ttaatcggtg tagttgttta tgtcatgaaa
     7921 cagcgaaaaa aataagtgga atttctcctt ttatgatgta aaacaattga ctgaggaaag
     7981 taaaaataga aggaaatgag accaatagat gtctcacttc cttctatctt tttatagatg
     8041 aggttctcct tgctgctttt gattggctgt tcttattact tgatgcagga cagctttttc
     8101 acaatctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct caacaaacgg
     8161 caaaatatga ggaactattt ttccgttttt ttctctaata ctcaggtcag gacgctgatt
     8221 tcacaacctc tcgataaacg gtgttcaaaa gccagtttct cggtagtaag tcaaatttat
     8281 gcaaaaatgt ggaaagccat ttttacaaaa tttttcttac tacttgaaac taagcagctt
     8341 tttcacaacc tctaatacat ggtattccag aagtaacttc ttcgaaaaat aagccgaaat
     8401 gtcacaaaaa tttgagaagc gattttccca aattccttct tatttcttga agctgaccac
     8461 ttctggcaca acctcttacc agattcgcac tcttttttct ggcgctagat acatagcgtc
     8521 ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg ttttttggtt gcacgtttgc
     8581 tcggagttta gcaggggcat ggacatcgat ttgcagtaaa agttgttggt attctttttt
     8641 tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat tcctcgatag aaagatctga
     8701 ttccgttttt gctgcttcca atgcacagct tagaccgcct gcatcagcaa tattttccga
     8761 aacggttaat tttccgttta cttttccgtc agcaaaaggc agaccatcga attcttcgat
     8821 cattgcttgt gctttgtttt gaaattgtgt aagatcttct tctgtccacc agttgttcaa
     8881 gttgccatat tcgtcaaata aagcgccgtt attatcaaat gcatgggaaa tctcatgagc
     8941 gataacagct ccgattccac cataattagc agagcttgat tgttctagac tgtaaaatgg
     9001 tgcttgaagg atcgctgccg gaaagacgat cacattccga aatggatgat aataagcatt
     9061 gaccgtgtca gcactcattt cccattctga acgatccact ggtttgttcc atttgctgaa
     9121 acgatctttt aatgtgagtt tgctgaaaac aagagtattt gaaagcaaag taccaccctg
     9181 atctgcaggt actgttttga actggtcgaa taatgctggg atcttatcgg ggtagcctac
     9241 ttggatacct aagttattta attttatgac ggctttttct tttgttgcat ccccaagcca
     9301 gtcattttct tctagacgtt ttttataaac ggcgatcatt tttttcacca tttgttctac
     9361 gtctttttta gcagcttcgc caaaatattt ttttccgtag taatcgccaa ctacttgatc
     9421 gaattgtcca gaggcaaggt aatacgctgc ttttttctga ggcattgctt cgtctgttcc
     9481 agacaatacg cgagaataga ttccgctgac ttggcggaat tcttctgata gataaccact
     9541 aagagaacgt actgttttaa tgatcatcca gcttttgaat aacggaaaat gagcttctgt
     9601 caggatgtca ttcaaatgat cgaagtacac tggatcagtt acgatcactt tatctggagt
     9661 tgtcccaatc aaacccgtga caagtttttt caagtccagt ttatctgtgt atttaataaa
     9721 tgtatcaaaa gattgcggat tatacatttt gctgtaatct gcgttttctt ctgcactttt
     9781 tacatgagga gcaatcaatt tatcaaattg cactgcttgt tccgtgattg cttctgcctg
     9841 acttttttct ttgccggcaa gctctaataa ctgaacagcc atatcaaaga aaacgcctat
     9901 tagttgtgca ccgttgggat ggtctgatgc ataatacgtc ttatctggaa ggaataaaga
     9961 aggcggataa gcaaagaagg cgttcgtttg cgcattcttc atatctgcat ctacatccag
    10021 attaaatggc aatggtaatg tatccaacag ccattctgga aattgcttat tcaattcgtc
    10081 gaaagactgc aatgattcaa ctttttcgat tataggttga agcggttgag catgcaactc
    10141 atttcttcgg tcataatcgt ttgccagttg ataatatgca aggaaatgtt tcattttttc
    10201 tgaagacact ttttctggat gggcaagcag atcgtctgtt tctcccatca aaagttggtc
    10261 gatcccgtca actaaatctt ggaaaccgcc tgtagctggt ttatctgcag ggatttttgc
    10321 tgttttttgc cattcttcat tgactgcttc atagaaatct tgtttcaatc gttctttgtt
    10381 cattcatcga tcgctccttt tttgtttcgt gtgcccatta tcttacaaaa tgaaggaatt
    10441 aacaaataga acataaaaaa ttgaaaattc gaacaatcaa actgatttgt ttttttcaat
    10501 ccttcactta taatgaaaaa aaggaggcga tcccaactga atcaagtagt ggataaacct
    10561 ttatatttca aagta
//
LOCUS       DACDIP010000083        10426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDIP010000083 DACDIP010000000
VERSION     DACDIP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.083"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..62)
                     /locus_tag="IV257_002430"
     CDS             complement(<1..62)
                     /locus_tag="IV257_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8815518.1"
                     /translation="MSIEEAILLDTLRCRLNFLRT"
     gene            complement(132..386)
                     /locus_tag="IV257_002431"
     CDS             complement(132..386)
                     /locus_tag="IV257_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815519.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(550..873)
                     /locus_tag="IV257_002432"
     CDS             complement(550..873)
                     /locus_tag="IV257_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815520.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            1060..1133
                     /locus_tag="IV257_002433"
     tRNA            1060..1133
                     /locus_tag="IV257_002433"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1094..1096,aa:Ile,seq:cat)
     gene            complement(1518..2015)
                     /locus_tag="IV257_002434"
     CDS             complement(1518..2015)
                     /locus_tag="IV257_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8815521.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(2055..2864)
                     /locus_tag="IV257_002435"
     CDS             complement(2055..2864)
                     /locus_tag="IV257_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8815522.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            3198..4055
                     /locus_tag="IV257_002436"
     CDS             3198..4055
                     /locus_tag="IV257_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8815523.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            4048..4950
                     /locus_tag="IV257_002437"
     CDS             4048..4950
                     /locus_tag="IV257_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8815524.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            4983..5333
                     /locus_tag="IV257_002438"
     CDS             4983..5333
                     /locus_tag="IV257_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8815525.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            5367..6305
                     /locus_tag="IV257_002439"
     CDS             5367..6305
                     /locus_tag="IV257_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8815526.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            6383..7276
                     /locus_tag="IV257_002440"
     CDS             6383..7276
                     /locus_tag="IV257_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8815527.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            7314..7877
                     /gene="amaP"
                     /locus_tag="IV257_002441"
     CDS             7314..7877
                     /gene="amaP"
                     /locus_tag="IV257_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8815528.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            7891..8079
                     /locus_tag="IV257_002442"
     CDS             7891..8079
                     /locus_tag="IV257_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8815529.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            8092..8994
                     /locus_tag="IV257_002443"
     CDS             8092..8994
                     /locus_tag="IV257_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8815530.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            9026..9268
                     /locus_tag="IV257_002444"
     CDS             9026..9268
                     /locus_tag="IV257_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8815531.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(9524..9895)
                     /locus_tag="IV257_002445"
     CDS             complement(9524..9895)
                     /locus_tag="IV257_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815532.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3728 a         1774 c         1965 g         2959 t
ORIGIN      
        1 gttcttaaga aattcagacg acaacgtaaa gtatccagta aaatagcttc ttcgatactc
       61 atttaatcac ctttcattat agtagaatag ttataatgac tatagaaagc tacacatgca
      121 atcaatagta ctcaatgttt tttatttaaa cgtatcaaat aaagaatgac gatcaataaa
      181 gacaaaacca gtcctgataa ttcttctata aaaacactat cgggttcaaa gtagttgtaa
      241 ttaaaaactt tccacataaa gtagatagcc ggaagagtca aaatagctaa ataaaaccgt
      301 tctttgttcc aattcataag ataaacaata cttgcgacca gtgcgaccgc aggcacccaa
      361 aacaaaactc tcaccaacgt aatcatgtaa cggactcctc aacgattcat ataataaaag
      421 ataacatgtt attgcataca tcacaaatga aatgctagga aaataaaaga agaatagatc
      481 aatgaaaaat gaaaaagcag aacagttttc tgctgataag caggaactgt tccgctgaat
      541 gtgtcttatc tatcgagaaa aaatcaatgg attgatatct aagtaattca atacatataa
      601 aaagaggacg atcgcattca ctaaaaagga aagaatgatg atcccatagc gaatggaaac
      661 atatttaaca aatacttttg tttgaatacg ggcatatctt tttcctttat agctgagcat
      721 catgacaaga agcaaaaggg taatcacacc ttgaaaaatc attccaataa acagaattcc
      781 tgaggtttga taatttaatt ggagaaaata agctgctgcc gcaactaaat cgaataaaat
      841 cgcaataaat ggcaaaataa tcactcctaa cattgagaat aaataaaaga ataacatttt
      901 ttctaaaata agcaaataaa aattaaagta acaaaatagt tatgacacaa gtaaaagaaa
      961 taagaataac aatattattt tacttatatt ttgaaacttg tgagagtttg tcattcgtgc
     1021 tatagttgaa aagtaacaaa aataaatttt atttatgatg tacctttagc tcagttggtt
     1081 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacaaggt acataagagg
     1141 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat
     1201 gtttttactt ggctgttctt attactgcag atgcaagctt ttgaataccg tttaaggaag
     1261 aggttgtgaa atcagcgttt tgacctgagt attagcttaa caaacggaaa aatagttcct
     1321 catattttgc cgtttgttaa gctaataccg agggtcagct gattgaacac catttaagaa
     1381 agaggtcgtg agcctgccgt ttcactctgt gtcataaaaa agccaatcta ctttgataga
     1441 aaaagcacgt aggactaaaa cagttctgcg tgcttttttt attgtgtgtt aaatatcgta
     1501 aatgaagata gatattttta tgattcgacg atagagacgt tgaaatcttt caacagctct
     1561 aaagcggatt ctccgtgttt tctactggag actaacattg gatcaacaaa aaagtctgct
     1621 tcaggaaaag cactctgcag gcctatcaga ttagagagta cacacaagtt tgtttcaatc
     1681 ccgactactt cgatctcttt ttgttcttct ttgctttcaa ataaagtttc ctggatttca
     1741 gctaaagttt caggtggaat cgtaaagtaa tactttttaa tcactcgttc attttcttgt
     1801 ggagaaagaa gagggatcaa ttgcaaatct tctcgttctt cttctttgtc cttcagttca
     1861 actggtatat cccgtgtgaa gagaatatat tcattatttt ggcgtgcttt agctaatctt
     1921 tgagcaatac gttctgccaa ttgatctgac tctggaatat aaaattcgct gtttggatca
     1981 agaatatgat tttgcatatc aataataact aacattttct ttcctcctac attcatatcg
     2041 tcattttatt ctttttaata gaagtttttt aaaatataag caacaccagc ttcttcattc
     2101 gtcagtgtta cttggtttgc aatttctttt acttgtcgct gtgcattccc catcgcaata
     2161 ctcaacccgc tgtaattcaa catagcaatg tcgttttctt gatcgccaaa agccatgatg
     2221 tcactcgatt cgattccaag gtaatccgcc agaaaaacaa gtgaatgagc tttagtgata
     2281 cctgtaggcg tgatttctaa gaaatgcgga tcagagaata ctgattggat accatctgct
     2341 tgcagatctc tttgtagctt ctgtagtttt tcttcactct tatctgttaa tgctaattta
     2401 agatagctaa gagaaggatc ttccaacata tttaaaattt gatcaaaaga atggttctgt
     2461 aaattgaaat gttcataata agggtcatct ttgactagat ttgattcatc gaatgtcagc
     2521 tgatcatctt gggtttctcg gctagcaatg atggatattt tcgcttcttg agcatagaag
     2581 aaggcttttc tcagtgtatc gctatctatt tttgaacgtc tgagaacttt cgccattttt
     2641 ccatccttca tttcagcaat cagtgaacca ttggaagaaa tcaaatagcc accgattcct
     2701 aattcggaaa aaatatcttt agcagaaatg ctgttccttc cggtagcgac ggttacgatt
     2761 ccgccatttt catcaaattt ccgaagtgct tggatatttt cttctggtat ctttccattt
     2821 cgatcaagca aggtaccgtc taaatcaatc gctgctagtt tcattttatc ttcctacttt
     2881 ctttttacat atctattttc ctgttacctt aagattaaat gagaatttaa aaaaatgaaa
     2941 ctaacttgct ttgtcattat gaattttttc aaaaaacatt cttaagaaaa aggtggctat
     3001 atatagtagt ttttggtcaa tacctttgaa taaaaagcca tgaaaaacga atagttagcg
     3061 cttaccgatg cgttttgtga taaaaaaatt ttaaaatatt caatgaaaac aaagcatata
     3121 ttttgcttta aaaccaaatg aggaatattc tagatgtaga acaggttaga aaaagattag
     3181 aaaaaggagt gattttgatg gagaagagat acggcgtagt tcctgtggta actgcttcag
     3241 acgaaaatta tgctccttac ttgagcgtga tgatcgcaac tgcattagaa aattgcaata
     3301 aaacaagacg cataaaattt tatgtgattg acgatggtct atcagaatat agtaaagaag
     3361 gattggaaga aacggtaaat aaatattcaa gtaatgcaag tatccaattc ctgacagtgg
     3421 aaaaagatat ctacgaagac ttcctagtca gtgaccatat cacaactacc gcttacttaa
     3481 gaatttcttt accaaactta ttagctaaag aggattataa gaaagtcctt tacttagatt
     3541 cagatgtgtt agtactagac gatatcgtaa aactatatga tgagccgtta aatggaaaaa
     3601 caatcggcgc tattatcgat ccaggccaag tgaaagcttt ggaaagatta ggaatcgatt
     3661 ccgatgactt gtacttcaat tcaggagtga tggtgattga cattgatcaa tggaataaaa
     3721 aagaaattac agaaaaaact atccattacc tcagtgaaaa tggcgatcga atcatctatc
     3781 acgaccaaga tgctttgaat gctgtacttt atgaagattg ggaacaactt catccaaaat
     3841 ggaacatgca gacttcgctg atttttgaac gtcatccagc accaaatgaa aaatatgaac
     3901 gtcagtataa agaaggaaat gaaaaacctt cgatcgttca ctttacagga catgataaac
     3961 cttggaatac gttgaaagat catccgtata ccaatttata cttaaaaaaa ttagcacaca
     4021 gtactctaac gaaagtaggc gagatcaatg aataaaaaag aaatagcagt agtcgcaagt
     4081 tgtaacacaa aattcgtacc acatttagct gcattatttg tctcagtatt agataattgc
     4141 aacccgtcta aatttgttcg gttctatgtg attgacgatg atatcgattt tgaaagtaaa
     4201 cagcttttac gcttctcagt gaaaaacgca cgaatgaact cagatgtgga attcttgaaa
     4261 atcaataaag aattctttac aaatgtggta atcagcgatc gtattccaga aacagcttat
     4321 tatcgtattg cgattccgga attgttccgc ggcacagaag ttgaacgtat cttatatatg
     4381 gattgcgaca tgatcgcttt acaagatatc tctaaattat ggcgtcttga cttcggtgat
     4441 tcaatcgtag cagctgttga agacgctggg ttccatcaac gactggaaaa aatgaaaatt
     4501 tcagctaaat caatgcgtta tttcaactca ggtttgatgc tgatcaatgt gaaaaaatgg
     4561 ttagatgaaa atattacgca aaaagtatta gatttcatcg aacataatcc agaaaaatta
     4621 cgattccatg accaagatgc attaaatgct atcttacatg accgttggtt accacttcat
     4681 ccgcgttgga atgcacaagg ctatatcatg gcaaaagcaa aaaaacatcc aacagcagca
     4741 ggtgaacgtg aatacgaaga aactcgcaac aatccttaca ttatccattt ttcaggacat
     4801 gtgaaaccgt ggagcaagga ttttgaaggg ccaacgaaga aatattacga aaaatatgca
     4861 gggatgacgg ctttccgttg tgtagctaaa ttccctaaat atccaaaata tgcaaaagtt
     4921 caaccaaaaa cagatgtagt tgttaaataa tagaagacaa caactgaaga gggggagtag
     4981 gaatggaaat tacaatgtct aaaaatgcag tagagacgtt aattgaaaag gttggggaaa
     5041 atacaaaaat cgcactcgcg cttatcaatg attccgatcc atttctaagg gacaaaggtg
     5101 cttttgcaaa aggcagcttt tttcaaatca ttccttttgt ttcggaattt ggagaatatg
     5161 cgactaaaat cgagcatcca ttattggaca tctatacatc taaattagag caaaattatt
     5221 tcggtaaacg attgaacatg gacttcaaca aacagcttga cagcttttca ttagaaaatg
     5281 aaatcgcagt actcgattat aatatcaaat taaagaactg cttcttcagc taatagtttc
     5341 gagaaagtcc aaaaaggagg tatacattga gatactactt tgtggatgaa gaaaagaatg
     5401 taatagaaac agatgaaaaa aaatataatt ggctcgtact tgatagtgca aagcccgaag
     5461 agattgagca agcaatcgaa gaattcgagc ttcctgatga tattttcgta ggaaccgaat
     5521 atccggaaga agtttctcgg atggagcgcc tttatgagac gaaactagag catccatttt
     5581 ctttggtagt gattaatcta gatcagtcag ataatcgtat cgaaaaaaaa ttaacgccaa
     5641 tatcttttgt actttccgat aatctcttga ttacttgtat cgatagaaaa accgaatttg
     5701 tggatcggct gctgacaaag catggaaatc atttaaactc gtttgaaaag atcatcacgt
     5761 atacattatt agatatttac acccattacg tgaaagaact aagagagatg aaaaagcgaa
     5821 ttgacgcttt ggataaagca gctcgtaaaa caacggaaaa tgaagaactg tataaacagg
     5881 cagatcttga aagagacatc gtatatatcg atcatacctt aagagatcaa cgggaaacaa
     5941 tggatcaatt atggaattct gaaactttca tcaaacgttt agacgatgaa cagctactct
     6001 atgatgtcca tttgagacag cgacaaacag aaaaaatgat cgagatctat cgagatttat
     6061 tagagagtat tggtgatcta ttcaacggga tgatggacaa taatctaaat catatcatga
     6121 aatatctaga ttcagctgca ttaatcattt ccgttccggc gttgttcgca gggatttggg
     6181 gaatgaatac aggaggatta ccgggtaaaa gttctgctct aggctttata ctcgtagtct
     6241 ttggctcttt gatagcagca gtagttttag gttaccattt ataccggaaa gattatacaa
     6301 attaaagaat ctatttaaaa caggtatcaa acaaagaaat ttgttttggc aatacaattt
     6361 aaaaactagg aggaatacag caatgacaga accaaaatta atcgacccgc gaaacttgta
     6421 ccatacggag aaatttccta aacaagatca ggatacacca gctttacagc agaatatgat
     6481 tcctgttcct gattgtggag aagaatccta tgaaggcaat aatcaactag aaaaccgccg
     6541 tgtactaatc actggtggag attcagggat cggtcgtgca gcagcgattg catttgctag
     6601 agaaggggca gatatcgctc ttcaattctt cccaggagaa gaaaaagatg cagaggaagt
     6661 agcaaaatac gtgaaagttg caggacgtaa gattgtctta ctaccttttg atcttcgtga
     6721 cgaacaagca cctaaagaaa tcatagataa agccgtgagt gaacttggcg ggctagatac
     6781 gatggtacta aacgcaggac agcaaatttc ttgtcagtct attgctgagt taccacttga
     6841 gcaagtcaaa gacacattta tgataaacat catcgctatg tttgctttag tgaaagaagc
     6901 tgttccgcat atcccagcag gaggagcaat cgtaacaaca acttctgttc aagcattcaa
     6961 tccaagcgaa catttattag attacgcagc aacaaaagct tcaatcgcta actttacgat
     7021 cggtttagca aaacagcttg cacctaaagg aatccgtgta aatggtgtag cacctggtcc
     7081 aatctggaca ccattacaac tagacgaagg acaaccttct gattcgatcc ctgaatttgg
     7141 tcagcaaagt ttactagaac gtgcaggtca gccaggagaa ttagcaccag tttatgtttt
     7201 cttagcatcg aataaagcaa gttatgttac agcccaaatt tacggagtaa ctggtggaga
     7261 agcgatcaac ttataagaag ttgatcgttt cacccctatg aggaggtgcg aaaatgaata
     7321 aaggtgtaaa aacgatcgga gtgatcggaa cattactttt gctatctgtg cttttgtttg
     7381 cgatactatt aagtgcaacc tatccattgc cttttcagct agagaagttt cgattctttt
     7441 ccatcactaa ttcatacgtg caacaatatg tattttggct tgccgttgca tttgctattt
     7501 tgacaattat cacgttactt gttttcctgt tttatccaaa aacaatcgga aactttctac
     7561 tgaaacatgg tgatggggag ttgacgttgg ataaaaaagc aattgaagga atggttcgtt
     7621 ctcacttgca tgaagatgaa ttcatccatt caccaaaagt aaatgtcaaa gcaacgaaaa
     7681 acaagatcgc cgtcaatgta aaaggggagt tgaaacgaac ttcagcactc attggtaaaa
     7741 caggaacatt gatgaaagaa atcgaaaatg aagtacaaca catattggga acaaaagagc
     7801 cagtgaaagt atcggtgaaa tacacaggct ttgaagaacc agctaaacga agcaatgacc
     7861 gcaaacgagt agaatagggg gggaccaaaa atgaaagaac tattttcgac atacaagctc
     7921 cctatcatct ttggggtact tggattgata ttagccgttt tatggatcac cgtgggcttt
     7981 ttcaagacct tattgatctt gatcatggca gcgatcggcg tcgctatcgg tttttatctg
     8041 aaacaaacga aaatcttaga tcactatttt aaatcttagg aggaaacatc catgaataac
     8101 aataacaaaa ataataacaa taaaatgaac aaccaaaaac cagtgtctgc acatccagac
     8161 ccaacgacac caaaccaatc aaatggtgtg aacgatgaaa agaacaaaac agcaccagta
     8221 tctgcacatc cagatccagt agtccctaac gttcaacaag gagtagaaga gaagaaacat
     8281 gatgcagaaa agcatgcacc tcatcatcca ggaactccag aatctaaagt tccagaacaa
     8341 catgatgcaa tcgatccttc taaacctgtg gaagagcaaa aacatccttc tgaccatgcg
     8401 aaagcacaag aaaaacttag ttctgaccac acaaacggac aaggaaaact tggttctgat
     8461 catacaaata accaaggaaa gcatgattct gaacaggaag attcaatgcg cggtgagctg
     8521 aattatgaag ataaagttgt gcaaaaaatc atcggaatcg caattgaaca agtagacggt
     8581 ttattatctg caaacggtgg tttcttctct aatgtggcag gaaaacttgt gaatacagat
     8641 aatgtaacgg ctgggatcga aacagaagtc ggtaaaaaac aagtagcagt tgatatggat
     8701 gtcattgtgg aatatggcaa agatatcgaa aaaatctttg aagaaatgca agaagtaatc
     8761 ggcaaagaag taaaaaatat gacacattta gaagtgatcg aagtcaacgc taacgtagtg
     8821 gacatcaaaa caaaagaaga atttgaaaaa gatcaagaaa cggtgcaaga caaagtaact
     8881 gacgcagcga aaaaaaccgg tgaatttgct tcgaatcaaa cagataaagt aaaatcagca
     8941 gctggaagcg gcgcacaaaa agtaaaagaa aatacagaac cacgggttca gtaataagga
     9001 agtaaaacat taggaggaac gaaacatggg atttatttgg tcacttatcg tcggaggaat
     9061 tatcggagca attgcaggag caatcacgaa taaaggttct tcgatgggga ttattgcaaa
     9121 tgtcgtagca ggtttagttg gatcagcact tggtcaagca ttattaggtt cttggggacc
     9181 aagtattgca ggaatggcaa tcgtgccatc attgattggt gccatcattt tagtactaat
     9241 cgtatcattc tttgctcgaa aactgtaaaa agacttttaa aaagaggtcg tgaaaaagct
     9301 gttcttcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     9361 ttgggcatat tatcgaagat gcaagctttt gaacactgtt tatacggaat aagaggctgt
     9421 gacaagactt ttgtcacagc ctcttattta tgcgggcaaa ttcccaatta gatttttaaa
     9481 agtttttcct taaatgtgtc tttattgata cagagaagct tctttaataa aacaatgcct
     9541 ccctaaattt tcttgagata ggaaaggtga acatacaaaa taatacgacc actttgaaaa
     9601 cccagatgat ccacaaatgg atcgtatgat actgatgaag taaaaagcaa tggatcagaa
     9661 actcaaagag attgaaaaag acaaggaaga aataatatct cttttttact cttgtctgtt
     9721 ttagccaata atagctcgta aagtaatcta gacaaacaaa tagaagtaaa caaagtttcg
     9781 taagagcagc aatcgtatca tgttgctctg ctagacgatt gacgagcagg aaaaatgttg
     9841 gtaatggaat atagatcacg agtaatgaaa gaaaaaagta aacaataaat ggcatactat
     9901 tcctcctaat tgtgtgagtt cgccctcatt ttaaaataac gatatttgtt tgtgaagctt
     9961 tttcgtgtcg taacaaatgt tatctaatgt atttttaaat aaataatcat tattatcaaa
    10021 ataatgttaa aaataaaaaa gaattgtcca tcactgttat tcaaatcatt ttatcaaaac
    10081 tacatagtaa atgttaaaaa gcaacatgac ataatatata gaaggcttta cagtatatat
    10141 gggagcttac taaaaaagaa tggagtaaga gttaacacaa tgggccatta gctgaagttt
    10201 taagcacttc aaggtatgaa acagaatggc ctgtaatact acatgattat ttagactgct
    10261 caaactgcta tcagtctggt ggaatgaata aagtattata ctaaaagcag atccaactac
    10321 aacgtaacag gaaaccagtt gtatcaggaa aagtgatagt acatggagaa gaaaaggccc
    10381 cctttgtatg agtcagtaca tatcttattt atagataaaa cggtat
//
LOCUS       DACDIP010000084        10023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDIP010000084 DACDIP010000000
VERSION     DACDIP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.084"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..722)
                     /locus_tag="IV257_002446"
     CDS             complement(<1..722)
                     /locus_tag="IV257_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8815533.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="IV257_002447"
     CDS             complement(727..1494)
                     /locus_tag="IV257_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8815534.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1983..2408
                     /gene="lacA"
                     /locus_tag="IV257_002448"
     CDS             1983..2408
                     /gene="lacA"
                     /locus_tag="IV257_002448"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8815535.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2425..2940
                     /gene="lacB"
                     /locus_tag="IV257_002449"
     CDS             2425..2940
                     /gene="lacB"
                     /locus_tag="IV257_002449"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8815536.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2951..3883
                     /gene="lacC"
                     /locus_tag="IV257_002450"
     CDS             2951..3883
                     /gene="lacC"
                     /locus_tag="IV257_002450"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8815537.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3886..4866
                     /gene="lacD"
                     /locus_tag="IV257_002451"
     CDS             3886..4866
                     /gene="lacD"
                     /locus_tag="IV257_002451"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8815538.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4894..5211
                     /locus_tag="IV257_002452"
     CDS             4894..5211
                     /locus_tag="IV257_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8815539.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5211..6917
                     /locus_tag="IV257_002453"
     CDS             5211..6917
                     /locus_tag="IV257_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8815540.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7059..8465
                     /gene="lacG"
                     /locus_tag="IV257_002454"
     CDS             7059..8465
                     /gene="lacG"
                     /locus_tag="IV257_002454"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8815541.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8488..8761
                     /locus_tag="IV257_002455"
                     /pseudo
     CDS             8488..8761
                     /locus_tag="IV257_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            8782..9681
                     /locus_tag="IV257_002456"
     CDS             8782..9681
                     /locus_tag="IV257_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8815542.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3164 a         1690 c         2006 g         3163 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaacaaaaa ataaacaaaa aagtgttgct
     1861 ttttgtttga atgattgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     1921 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     1981 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     2041 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     2101 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     2161 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     2221 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     2281 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     2341 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     2401 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     2461 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     2521 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     2581 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     2641 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     2701 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     2761 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     2821 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     2881 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     2941 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     3001 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     3061 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     3121 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     3181 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     3241 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     3301 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     3361 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     3421 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     3481 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     3541 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     3601 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     3661 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     3721 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     3781 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     3841 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     3901 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     3961 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     4021 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     4081 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     4141 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     4201 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     4261 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     4321 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     4381 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     4441 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     4501 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     4561 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     4621 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     4681 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     4741 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     4801 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     4861 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     4921 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     4981 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     5041 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     5101 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     5161 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     5221 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     5281 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     5341 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     5401 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     5461 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     5521 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     5581 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     5641 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     5701 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     5761 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     5821 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     5881 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     5941 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     6001 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     6061 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     6121 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     6181 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     6241 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     6301 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     6361 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     6421 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     6481 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     6541 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     6601 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     6661 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     6721 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccgcaggtcg
     6781 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     6841 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     6901 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     6961 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     7021 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     7081 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     7141 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     7201 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     7261 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     7321 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
     7381 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
     7441 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
     7501 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
     7561 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
     7621 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
     7681 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
     7741 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
     7801 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
     7861 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
     7921 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
     7981 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
     8041 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
     8101 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgatt gaaaaatgat tatccaaact
     8161 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
     8221 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
     8281 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
     8341 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
     8401 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
     8461 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
     8521 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
     8581 cctgagcttg aagctaccct cgttgctaaa atgtggtaaa tggtatgttt taactggaaa
     8641 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
     8701 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
     8761 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
     8821 tttaaaacac ttggtggtca actgacttcg attaaagacg aggatgggat agagtatctc
     8881 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
     8941 agtttacgaa atgattgggc tatttataga cctcaagaaa gacctttttt tacaggtctt
     9001 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
     9061 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
     9121 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
     9181 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
     9241 tctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
     9301 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
     9361 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
     9421 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
     9481 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
     9541 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
     9601 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
     9661 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
     9721 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
     9781 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
     9841 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
     9901 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
     9961 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    10021 gtt
//
LOCUS       DACDIP010000085         9797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDIP010000085 DACDIP010000000
VERSION     DACDIP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.085"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(183..1031)
                     /locus_tag="IV257_002457"
     CDS             complement(183..1031)
                     /locus_tag="IV257_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8815543.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1195..2097
                     /gene="gnd"
                     /locus_tag="IV257_002458"
     CDS             1195..2097
                     /gene="gnd"
                     /locus_tag="IV257_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8815544.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2099..3100
                     /locus_tag="IV257_002459"
     CDS             2099..3100
                     /locus_tag="IV257_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8815545.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3097..3381
                     /locus_tag="IV257_002460"
     CDS             3097..3381
                     /locus_tag="IV257_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8815546.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3414..4211
                     /locus_tag="IV257_002461"
     CDS             3414..4211
                     /locus_tag="IV257_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8815547.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4225..5058
                     /locus_tag="IV257_002462"
     CDS             4225..5058
                     /locus_tag="IV257_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8815548.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5076..5933
                     /locus_tag="IV257_002463"
     CDS             5076..5933
                     /locus_tag="IV257_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8815549.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5955..6464
                     /locus_tag="IV257_002464"
     CDS             5955..6464
                     /locus_tag="IV257_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8815550.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6501..6902
                     /locus_tag="IV257_002465"
     CDS             6501..6902
                     /locus_tag="IV257_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815551.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7014..7367
                     /locus_tag="IV257_002466"
     CDS             7014..7367
                     /locus_tag="IV257_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815552.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7401..7844
                     /locus_tag="IV257_002467"
     CDS             7401..7844
                     /locus_tag="IV257_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8815553.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7861..8946
                     /gene="uxuA"
                     /locus_tag="IV257_002468"
     CDS             7861..8946
                     /gene="uxuA"
                     /locus_tag="IV257_002468"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8815554.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8939..9652
                     /locus_tag="IV257_002469"
     CDS             8939..9652
                     /locus_tag="IV257_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8815555.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3256 a         1553 c         2166 g         2822 t
ORIGIN      
        1 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
       61 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      121 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      181 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      241 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      301 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      361 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      421 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      481 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      541 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      601 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      661 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      721 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      781 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      841 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      901 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
      961 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1021 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1081 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1141 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1201 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1261 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1321 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1381 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1441 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1501 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1561 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1621 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1681 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1741 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1801 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1861 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1921 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     1981 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2041 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2101 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2161 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2221 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2281 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2341 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2401 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2461 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2521 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2581 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2641 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2701 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2761 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2821 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2881 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     2941 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3001 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3061 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3121 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3181 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3241 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3301 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3361 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3421 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3481 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3541 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3601 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3661 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3721 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3781 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3841 tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg
     3901 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     3961 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4021 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4081 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4141 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4201 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4261 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4321 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4381 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4441 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4501 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4561 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4621 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4681 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4741 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4801 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4861 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4921 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     4981 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5041 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5101 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5161 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5221 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5281 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5341 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5401 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5461 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5521 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5581 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5641 agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5701 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5761 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5821 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5881 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     5941 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6001 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6061 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6121 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6181 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6241 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6301 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6361 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6421 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6481 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6541 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6601 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6661 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6721 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6781 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6841 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6901 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     6961 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7021 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7081 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7141 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7201 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7261 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7321 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7381 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7441 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7501 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7561 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7621 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7681 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7741 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7801 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7861 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7921 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     7981 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8041 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8101 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8161 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8221 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8281 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8341 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8401 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8461 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8521 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8581 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8641 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8701 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8761 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8821 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8881 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     8941 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9001 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9061 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9121 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9181 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9241 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9301 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9361 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9421 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9481 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9541 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9601 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9661 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9721 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9781 aaacgcatct actgtta
//
LOCUS       DACDIP010000086         8729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDIP010000086 DACDIP010000000
VERSION     DACDIP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.086"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..105
                     /locus_tag="IV257_002470"
     CDS             <1..105
                     /locus_tag="IV257_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8815556.1"
                     /translation="DFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(173..466)
                     /locus_tag="IV257_002471"
     CDS             complement(173..466)
                     /locus_tag="IV257_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8815557.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(494..1159)
                     /locus_tag="IV257_002472"
     CDS             complement(494..1159)
                     /locus_tag="IV257_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8815558.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1299..1931)
                     /locus_tag="IV257_002473"
     CDS             complement(1299..1931)
                     /locus_tag="IV257_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8815559.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1938..3005)
                     /locus_tag="IV257_002474"
     CDS             complement(1938..3005)
                     /locus_tag="IV257_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8815560.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(3002..3733)
                     /locus_tag="IV257_002475"
     CDS             complement(3002..3733)
                     /locus_tag="IV257_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8815561.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            complement(3901..4380)
                     /gene="greA"
                     /locus_tag="IV257_002476"
     CDS             complement(3901..4380)
                     /gene="greA"
                     /locus_tag="IV257_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8815562.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4516..5691)
                     /gene="mltG"
                     /locus_tag="IV257_002477"
     CDS             complement(4516..5691)
                     /gene="mltG"
                     /locus_tag="IV257_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8815563.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5856..7016
                     /locus_tag="IV257_002478"
     CDS             5856..7016
                     /locus_tag="IV257_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8815564.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(7056..8003)
                     /locus_tag="IV257_002479"
     CDS             complement(7056..8003)
                     /locus_tag="IV257_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8815565.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(8036..>8729)
                     /locus_tag="IV257_002480"
     CDS             complement(8036..>8729)
                     /locus_tag="IV257_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8815566.1"
                     /translation="VVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNATKILFQELL
                     PHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNARITIWKSAW
                     EIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGILGIIPFMAF
                     LIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTVGTFVFWLFL
                     GRVNSKNNSSYF"
BASE COUNT         2641 a         1798 c         1350 g         2940 t
ORIGIN      
        1 gattttcaaa agaaattaat ggctattttt ttaaccaatc tgaaaagcta ctttgccacc
       61 catgagcttc aaaccaatca agtaaaattg tctgcaggat attaatagct gaaaacaata
      121 ttttcaaaag aaaaaagaga aggtgacaaa aattgtgtca ccctctcttt ttttattgtt
      181 ttttaaagtg ataggttggc tcctccaggt tgggatcata atcaacaagt aaatcatatc
      241 ctttaaagaa ccattcatct gcttcctcga taaaaaaaag tgtcccatcc atttccactt
      301 tctttacagg atgttctggc tgttcaactg acattccaat cgaaaaacca tcatgaacct
      361 ctgttttgcc gtagactttt ccaaaaaaac gtattccgta agtttctggc agatcaattt
      421 ctgagtcaaa ccacttttgt gcttttggcg ttaccgttaa tttcatccca acaacctact
      481 ttctcttttc tttctattga ttcatcttat cgtctagaat atccacatcg cctgtctctc
      541 caataaccag tagattatca ttttcacgaa catattcatc tgctgcagga gaagcgataa
      601 ccgtttgatc tactcttcgt atggcgacta ctgttaagtc aaaacgttgc ctgaaattta
      661 attcagctaa tgttttgttg aaaaatttag gattggagac tcggatttct gctaaagaat
      721 atttatctga cagctctaag tagtctagaa tattctttga tactaagtta tgtgcaatcc
      781 gtatccccat gtctcgctca gggtgtacga cacgatctgc tccgatcttc tctagtacac
      841 gggcatggta ctcattttgg gcttttgcta aaatattcgg tacgcccatt tcctttacca
      901 tcagagtaac taagatgctg gcttggatat cttctccaat agcgacaatt acatgatcga
      961 aatttcttat acctaatgaa cgaagtgtca tctcatcctg tgcatttgcc acaacagcat
     1021 gggtggctat attcatatat tcattgactc gatcttcatt actgtcaata gccagtactt
     1081 cttgtccagc ttcgatcagt gtacggcaga tgcttccgcc aaatcgtcca aggcctacaa
     1141 ttgcaaaatt ttgtttcacc attttcctcc tttagtatct tcttttctca gtataacaaa
     1201 gcctttccaa aaacaaaagc aagcatcatt attgtaaaaa agccaagaaa aataatagtt
     1261 attttctcag cttttgatta tcctctttat catcgttttt atttcaccaa accatgttta
     1321 aacgcataga tagctgcttg agttcggtct tctacctcta acttagctaa tatattcgac
     1381 acatgcgttt tgactgtttt cagtgtgata aacaattcat ccgcgatttc ctgattactt
     1441 ttcccttcag aaatcagcat caaaatttca ttttctcgat tcgttaattc ttcatgcaat
     1501 actggatcat ttcgccgact cattttttcc atcatttttg ttgtcacttc aggttctagt
     1561 actcgctcgc cgcgttgcgt agcccttata gcatcagcta tttcatgtgc tgtagacgtc
     1621 ttcaacaaat atcctgctgc tcctgcttca attgcaggat aaactttttc atcatcaata
     1681 aaacttgtga cgataatgat tttggctttt ggccaatctt ttaagattgc ttttgttgat
     1741 tcgatgccat ccatttcttc catgactaga tccattaaaa tgacatccgg tcgaagtgcc
     1801 atcgcctttt catagccttg acgtccattt tcagcttctc caatcacttc aatgtcttct
     1861 tggatagata aataagaaga gacgcctaag cgtaccattt catggtcatc tactaataaa
     1921 acttttatca tctttatcta tgcctccttc atcaaaggga ccttggtctc cacgctcgta
     1981 ccttgccctt taaaactaat gatttttact gttccaccaa taccagctac tcgttctttg
     2041 atattgttca atccataact tcccgttttg gtttcattca tattaaatcc tgtcccgtcg
     2101 tcaatgatac gtaataaaag attgttatct attttatgca aatagacttc taattcatta
     2161 gccttagcat gtcttaatgt gtttgataac aattcttgaa cgattcggaa caagtgatcc
     2221 tcaatggaac tagttaattt tacatcttct acctcccatt ttagcgaaat ctggatttta
     2281 ttctgcaatt ccttcaataa ctgctcaatg ccttgtttta aacttttttc ttctaaattg
     2341 actggacgca gatgaagaag cagcgcacgc atctcagact gggaagcgtt gatgatctct
     2401 gccaccattt tcaactgctt acggaacatt tctggtgtct cacttttttc tgcttgctcc
     2461 gtcaatgccg acatcatcat catcgctgca aataattgct gtgaaactga atcatgaagc
     2521 tcacgagcta atcgatgcct ttctaatttt aaaatctctt ctttggtttg tccatctaca
     2581 tattgaggac ggctagtcat aatctgtaat tcactggata cttcgatcat tttttctttt
     2641 attttagtaa tttccatatc aatatctttg atgtacagat catccgacgc gcttgggatc
     2701 ggctgatgca aaagtttgga ttcataattg ccagaagaaa gtgcacgaag cttttcttcg
     2761 attttcccgt attttgcctt ttgaataagt gataacaata gaaatacgat caatcctaca
     2821 cctaatgaaa ttgccatcaa atggaagata agcggaacgt ataataaacg cgcacgcagc
     2881 aattctcccc accaatgact ttggttactg gcataaaaat aagtgaaaag tgaaaataga
     2941 ataataagga aagccgcaat ccccgaataa acagctagca ttgctttgga tattcttccc
     3001 atcatattcg tatcacctcg atatctccaa ccaaagtatt ggtaatgatc ttcaatctgc
     3061 gaggattttc atcataatca ctgctgcaga ttttgatctg ttcgtttttt aaggaaaact
     3121 gctcttcttc aaacaagaca tttcctacta atgtcgcatg ctctaaacga atagccactc
     3181 ccaggggtac taagatccgt gttctgccta tcccttttct tacaatcaca atattatcat
     3241 cttttggcaa aagtgtattg cccaaatcga tgatagtatc accagaaatg atggcaatgt
     3301 tgatatcatc ccattcatag acgtcgtttc caatacgttg gttaccaaat aattgctgtc
     3361 ttttacgctc attattatgt gtttttaatt gctctgtttg gaccatcatg atctgttttt
     3421 ttcgccaaaa agcatttttt gtcaaatcta ttcctgaaat ctctacacct ttcagcccga
     3481 taaataaaac ggccaataca accatcatcc ataaagcagg actgttgact aagctaaaaa
     3541 aaatgaccaa gctccctaag atcaattgaa aattttgaaa tttagtacga ggatatttgc
     3601 gcatagctag atatatattg aaaataccga atattactaa aagcaaaagt ccagtattgt
     3661 ttacgatttg ccaaacagca aataacagca gcagtgcttc gactaccaca aaaaaacgcc
     3721 aagaactatt catcgctttt tctccctcct gacacattct catttcatca tacctatttt
     3781 tttgactgtt ggctatagga ctttagatgt aagatcgttc agtgaaacct gcaatactca
     3841 aagagaaaag atctagagtg cgtcatcagt aagatgaact ctctagatct ttcagtcaga
     3901 ctacgcttgt tctactttga cgattttaac taacatatcg ccacctggtg tagcaatggt
     3961 tacttcatca tttttacgtt tgccaatcaa tgcttgcgcg ataggagaat cattggaaat
     4021 ctttccagaa aacgggtctg cttcagcact tccgacaatc gtgtactctt cttcatctcc
     4081 atcaggcaac tcgatgaacg taactgtttt accgatcgat acctcatctg catctacgcc
     4141 gtcattatcg atgatttgtg caaaacggat catattttca attgttgtga ttcttccttc
     4201 aacaaatgct tgttcatctt tagctgattc gtattcggag ttttcggaaa gatctccgaa
     4261 accgcgagcg attttgattc gttcaatgat ttctccgcgt ttaactgttt ttagttcttc
     4321 taattctttt tctaattttt ctttaccttc tagagtcatt ggataaactt tctctaccat
     4381 gtgtaacgtc tccttttttc gataaaatac cgcaaaaggt aatcttttga ggaatatagt
     4441 ccaaaaaaga ttaccacgct tttcttaaaa tgtaaatgtt tttctaaaaa tgatcaatga
     4501 ctagcccctg tttttctaag aattatttac atatttttgc actagttcca tatgttcttc
     4561 atacgtttta gcgaaataaa catttccagt gctcgtatct gctacgaaat aataatagtc
     4621 attttctgct ggttctaata ctgccttgat tgcctcttcg cttggattgt caaaaggacc
     4681 aggcccatag ccggtattgg tatacaaatt gtatggtgaa tctacagctg tatcttcata
     4741 cgtcaccaat tctttatgtt ctcctaatgc atataaaaca gagatatctg actgaagtgg
     4801 catatttgcc ttgattcggt taaagaagac ttgtgcaata tttttacgat cattttcttt
     4861 tacgccttct ttttcaacta gagaagccaa agttaaaact tcttgtacag tcaaattttt
     4921 ctgtttgata gctgaataat agttttgcat gaccgtattt gttttgttca ccatctcaat
     4981 cacaagatcc ttaagcgaag tactcgtgta gtaatcataa gtagctggga ataagtagcc
     5041 ttccaaacgg tattttgtgt tctcagcttt agaggcactt tccaacaatt ctgggaattt
     5101 ttgatacaac tcgttaaaga aagtctcatc atttactaaa tctaaaaatt ctttcttgtc
     5161 tttaccagtt accttcgcta cccgctcagc aatctgatcg atgtcgtagc cttctggaat
     5221 ggcaattttc ccatctgcaa cttttgttgg ctcagatgtg ccgccttctt gcaactgctt
     5281 gccaatctca tcaagagtca tattaggcgc taactgataa taaccagctt gaaagccagt
     5341 taagttttta aattttgtat aataattgaa aacaatccca cttttaataa tattgtcttt
     5401 ctccaaaatt tcgccaatct gcttattaga agagccgctt gggatttcca cttggactaa
     5461 ttggtcgtcc gatttatcta aaggcttcaa cccgctatct acataacgat atacggtaaa
     5521 cccgagaaat ccgccaataa tcaataatgt aagagcgatc actaaaatga tcttcctcac
     5581 gatcttatct tcttttttac gcagatttct ccgcttgtcg attatatctg ctgtagctgc
     5641 cttactggct tcttttttgt ctttgcttgg gtgttgtttg tttcctgaca aaacaactcc
     5701 tccttactat catcttcgta actctgttta ttatacatga aatttctact gccgtgaatt
     5761 attttaccaa atattatgtc aaaattgtaa gtggcaaatg ctctttttga ggattacaat
     5821 gaaaaatagt taaaaatgga taggaaggat ttaatatggc aaaaggaaaa catcaaaaaa
     5881 aacagcagat ctctctttct tttctcgcag ttttgttgct ggctgtcagc ttcttttcaa
     5941 caggcatcat tacaggatgg caattaacaa aacaacctca accacaaatt tataaaacgg
     6001 aacccgcaca acttgcttcc aatttaacat ctaatgagca gatgcttgcc agcaaaattg
     6061 atcaattact gcaaaaatca gattatatcg gttcgattta tgtacgcaaa aataatcgag
     6121 tgatattaga aaaaggatat gggtacgcaa atctacgcat aaaagaatcg aattctcctt
     6181 ctctttacta tcaaatcggt tcgatccaaa aagcgatgac tgctttactt atcctaaaac
     6241 aaattcaatc tggcaagata acttttgaca caaagctatc tgaattttat cctcagattc
     6301 ctggaagtca gcaaatctca atcaaaaata tgctttacat gcgctctggt cttcatcgaa
     6361 ccgcttctcc aaaaaaacct atgtcagata gtgagatcat tcagtttgcc cttcatcatt
     6421 tgaaatttac tggttatgat acgtatcatt atgaaccatt aaattataca ttgcttacag
     6481 gggtattgat caaactaatg aatcagcctt atgaaactct tttaaaaaaa gaaatcattc
     6541 ggccactcca tctagaacat acagattttt atgaaaatgt aaaaaattcc cctcagcatg
     6601 ctgtatctta ccaaatgtct gcaactgacg attattcaaa agcattggtc gagcctgaaa
     6661 cagacatccg aaatgaatta ggcacaggaa atataagtat gtctgtttat gacttgaaca
     6721 aattctttac cagtgttctg tctggcgata ttataccaaa agaactactt ttctcattgt
     6781 ggcaaaataa tggtgataaa catccttaca gcggcggtgt ttacagtgga aatgattata
     6841 tccttgctca gggaaatatc aatcgattcc atgcagtagc ggcgatgaaa aaagacacaa
     6901 aagatgcaat cgtcatggaa agtaatatcc aatctgataa aaacatcaaa ttgccggcca
     6961 ctgatttaag aaatcagatt tacgaactga tggaaggtgt taatttaaaa agctaagtcg
     7021 agacaaaaaa accagcagga tgctggtttt ttgtttcaat cgatttctgt ctttaaataa
     7081 cgttccattc cgttcaatat atcaaatttg ggttgataac ctaaagaacg gatctttgaa
     7141 atatccgcta aagaatcacg aatatcccct gaacgctccg cttcatattt aagcgctagc
     7201 tctgttccta agatttgatc aattgaatga atcaattcaa gtagcgtagt gctttttcct
     7261 gtaccgacat tgaattgctg tcctaaagct ttttcttcct tagcaacaag taaaagtgct
     7321 tgtataacat cttctatata aacgaagtct ctagattggc taccatcacc aaataaagta
     7381 aattctgttt tttctcctgc caattgtttt ttgtatcggt ccactagaat cgaaataact
     7441 ccgctatatg gagagttagg attttgattt ggaccataaa cgttaaaaaa tcggacagca
     7501 ctagtcggaa catcatataa atgacagtaa tccaaaacat attgttctgc tgcaaactta
     7561 tcgatggcat atggtgttaa aggacggatg accgattctt ctttttttgg caacgttggt
     7621 tcatcgccat aaactgcagc tgatgaacta aataccaatc gttttaaacc agattgatat
     7681 tttcgtacca attcaagcaa taaaagcgca ctgtcaaaat ttacacgatg agtttctaga
     7741 ggacgttcta cagaatctgc cacactagct acagcagcta gatggaaaat ataatcgaaa
     7801 tggtattctt tcatgacctt ttccatcaaa tttttgtcag cgacatcccc ttcaataaac
     7861 gtgatgtgct tcgattcatc cagattctct tttttcccca tcgataaatc atcgatcaca
     7921 actacattgt tttcttttcc tagataattc gcgagcgtcg agccaatgaa gccggcgcca
     7981 ccggtaatca agtaattcaa cattttttct cctcttttca agataaaatt tattactaaa
     8041 aatagctaga attatttttg ctattaactc ttcctaaaaa tagccaaaaa acaaacgttc
     8101 ctacagtgtt ttcatagact aattcattgt taagagctgc gtatagtgca taagataaaa
     8161 taactaaaca gtaaacaaaa tgataatctt taccaatgca tttattatag atgattcgat
     8221 aaacattcag cacaaaactc tttattaaaa aagccatgaa aggaattatt cctagaatac
     8281 ctgtcgatgt taatacattc aagtatgcat tatgaatggc aattcctact ttcgcaatat
     8341 atccatctgg aataattgac tgtgcataag atactacatt tttcggagag actccgataa
     8401 gccaacttgt tttaaatatt tcccatgcac ttttccatat agtaattcta gcattagaaa
     8461 tatctgtatt ttcagctacg tctttcctaa caagagtatt atcaggtgtt tgagtattat
     8521 taatatgttg actattattc attaattctt tatgacgctc tgtaaaatta aaaaaattta
     8581 tttcttttaa atgaggtaat aattcttgaa aaagtatctt agttgcatta aacaaagcga
     8641 ataaaatcag aacagaacca atagaagata ctatagcaag gatatagttt tttaaatctt
     8701 tttttgttcc atttcgtttt aacacaaca
//
LOCUS       DACDIP010000087         8582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDIP010000087 DACDIP010000000
VERSION     DACDIP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.087"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..532
                     /locus_tag="IV257_002481"
     CDS             35..532
                     /locus_tag="IV257_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8815567.1"
                     /translation="MNNQIALAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1264)
                     /locus_tag="IV257_002482"
     CDS             complement(569..1264)
                     /locus_tag="IV257_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815568.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKRKRIMVPLPV"
     gene            1484..2263
                     /locus_tag="IV257_002483"
     CDS             1484..2263
                     /locus_tag="IV257_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815569.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            2304..3248
                     /locus_tag="IV257_002484"
     CDS             2304..3248
                     /locus_tag="IV257_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8815570.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            3264..4043
                     /locus_tag="IV257_002485"
     CDS             3264..4043
                     /locus_tag="IV257_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8815571.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            4056..4754
                     /locus_tag="IV257_002486"
     CDS             4056..4754
                     /locus_tag="IV257_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8815572.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            4782..5546
                     /locus_tag="IV257_002487"
     CDS             4782..5546
                     /locus_tag="IV257_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8815573.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            5559..7376
                     /locus_tag="IV257_002488"
     CDS             5559..7376
                     /locus_tag="IV257_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8815574.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            7390..>8582
                     /locus_tag="IV257_002489"
     CDS             7390..>8582
                     /locus_tag="IV257_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAP8815575.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINN"
BASE COUNT         2984 a         1536 c         1746 g         2316 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatcg cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ttttttgata aatcgctaat tccgtcacac cttttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgacc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttctatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagtatat tttgatgaac
     1261 gcatatgtag tcctcatttc ttgggagttt tgagactact atatgcgttt ttttgttgat
     1321 ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca cgggtttcta
     1381 tgaatatatt agtttaatta tgcttttaaa ataaaaagcg ctcattctga tgtgaatgaa
     1441 tcacatcttt atttatctat catcaatatt taggagtgaa tctatggaaa agccgttcgt
     1501 caagttattg gctactgtgg cggtaggcac aggcgctatt gtgatttgct tgtttggata
     1561 ccatctcaat aatcagcggc aacatcatca acggatcaat tacgcagaat ctgccatcac
     1621 caatcaaaaa gacaccgtga cctctttatc aaaagaagta gataagctgt acagtacaaa
     1681 agaaaaaatt ttcctaaatc ctgaaataac agaagaaaca gtttcaaatt tgtcacataa
     1741 actatcttct atcaaacttt cagcagatga ttatgacatc aaggaatcag aattacctaa
     1801 agaagctgct gcgatccaag aagaaaagaa agcagtctta acacagttag aggatgcaga
     1861 atcaaaactg aagatccaaa cggctgtcaa taaattattt actaaaaacg tatcaaactg
     1921 gcagcaagct gtggatgatg tcatcatcaa agaaaagttg gcaagtgcag atgtcgccca
     1981 tgtacgtgaa aatatgagct tttttaagga tagtgcttgg aaaacggtcg ttatgcaata
     2041 tctaggattc gctgataccc aaattgcaca agtcacgcaa ctagatcagc tattcgatac
     2101 gatgctgaaa gacggtcaag taacagctac tgctacttac gatcaatatt tgaccgcact
     2161 cagtctcatc gaacagatcc ggaatgaaaa aataagagcc gcatacgcgt caaaagcaga
     2221 aacagtcgct cagcaaatgg gctatagcaa tacgagctat taataagcga gaattcaagc
     2281 aaggctagca aacggaggaa actatggcaa aacgaagaag aagatcaaga gggaaaaaga
     2341 tatttctaat tattttagca attcttgtcg tattagtatt agcaatcgta ggagtaggag
     2401 caaaattata tctagatctc tcacattcta ttgaaaaaac ttacgaatca gtggaacgaa
     2461 cccaaggaga acgaacaaaa ccagttgatc tggacaaaca agaatctttc tctgttctat
     2521 tgatggggat tgatacggga gatttaggtc gggtcgatca aggaagatca gatacgatga
     2581 tggttgccac agtcagtcct gaagacaaac agacaacgat tgtcagcatt cctcgtgata
     2641 cgtatgtcga catcgttgga catgacacaa aagacaagat caaccacgct tacgcattcg
     2701 gcggagctgc catggcgatg gataccgtac aaaaatatgt agacatccca atcgatcact
     2761 atgtttcaat caatatgaaa ggtctaaaag aactcgttga tgcagttggt ggtatcgaag
     2821 tcaataatga cctcaccttc tcacaagatg gctatgattt tacgatcggt aaaatcacat
     2881 tgaacgggga tcaggcattg tcttattctc gtatgcgtta tgaagatcca aatggtgact
     2941 acggcagaca agaacgtcaa agaaagatcg tagaaggtgt agcgaaaaaa gtgttgagtc
     3001 tgaacggtgt aagcaactat caatccgttt tgaatgctct agagtcgaat atgaaaacag
     3061 acatgagctt tgatgaaatg aaaaagattg ctttagatta tcgggatgca tttggtacga
     3121 tcaagcaaga tcagctgcaa ggagaaggct tcatgcaaga caatatctca taccaaaaag
     3181 tcagcgatca agaacttgat cgtgtgaaaa aagaattaaa agatcaaatg aatctagaga
     3241 ataaatagga aagtggagaa aaaatggaag agacaatcag cttgaaagaa atttttgaaa
     3301 cattacgcaa acacgtgata acaatcctca tctcaatgtt tgctggcttg gccatctcag
     3361 gcatcgtgac gtttttcatc ttgacgccaa aatacagctc acaagcccaa ttgatcgtga
     3421 cactgccgca atcagatact gcaaatgtca atgatgtcaa tacgaacctg caaatgatca
     3481 atacgtataa ggatatgatc gtcagtgatc tagtactaaa tgaagtcaaa gatcgtttag
     3541 aaacagaaga caatgtaaaa atgactgctg gacaaatcaa agatgccatc tccgtcaatc
     3601 agtcacaaaa ttcacaaatg ttctcgatcc aagcaacaag cacaaatgca gtgacggctc
     3661 aacaaatcgc taatacaacg tcacaagtat tccaagagaa cgccaaagac gtgatgaatg
     3721 tcgataaaat ctcgatcatc tcaggtgcag ttgccaatac gacacctgtt tcgccaaata
     3781 ataaattgaa tctagtgatt ggcttagtgt taggaatgat ggtaggcgta ggtctagctt
     3841 tcttatttga attactcgac cggacagtaa aagatgaaaa atatgtgacg gatacattgg
     3901 gcttcccgat cttagggact gtaccagaaa tgtcagcgaa agaattgaat gctggtatcc
     3961 gtaaaaaagg accagctccg acacaaccaa aagctacaaa cgataaaaca gcgccatcac
     4021 gcagaagccg ctcaagagtc taggaggaaa gaaaaatggc acgaacacag aaacaaaaag
     4081 cagcccaaac tcatgcagtc agtttggtca cactcacaaa cccctcttca ccaatcgctg
     4141 aacaatatcg tacgattcgg acgaatatcc agtttgcctc cgcagctggt cagcaaatta
     4201 aaacgattgt tgtgacttca tcaggacctg gagaagggaa atcgaccact gcagcaaaca
     4261 ttgccgttgt ttttgccaaa tctggtcaac gggtgctgct agtcgatgca gacttacgta
     4321 agcccgtcat ctataaaacg ttcaagttga acaacgcaag cggattaagt accgttctta
     4381 gttcatcagg aagtgtagca gatgagatcc aacgaacacc agtcgagaat ttgtcgattc
     4441 tgccaagtgg acctaaacca ccaaatccat cagaactact aagttcgcca agaatggatc
     4501 agattttagc agaagcaaga caactatttg atgtagtgat cttcgacatg ccgccagtag
     4561 ttgccgtaac agatgcacag atcatgtctt ccaaaacgga tggcacctta ttggtcgtac
     4621 gtgaaaatat gtcacggaaa gaatcattga ctaaagcaaa agaattgctg gatatggtac
     4681 aagcgcgagt ccttggtgtt gtatacaatg gggcagaaca cagcaaagat gctggctatt
     4741 actacgggaa ttaagaaata aaaagagagg agacaagaga aatgatcgat ctacattgcc
     4801 atatcctgcc aggaatcgat gacggagcag aaaacataga tgccagtatc gccatggctg
     4861 aaaaagcgat tcaacaaggg atcacacata ttttatgcac accccatcat aataatggaa
     4921 aatacagcaa tgaaaaatca caagtcattt ctctagtcgc ttcgttacaa gcagaattag
     4981 agaaaagaca attgcctttg acgttattgg aaggacaaga agtacgtatt accggaacac
     5041 tgatcgaaga catcgaccga gatgaaattt tatttacaga tttagatgat acatatcttc
     5101 taatcgaatt tccgacactg gaagtgccgt tatatgcaga acagctcttt tttgagttgt
     5161 tacaactagg aaagacacca gtgatcgtcc atccagaacg caacgcgcat tttcgtaaag
     5221 acccgaatca tatgctgcca ttcctagata tgggatgctt ggcccaactc accgcaccaa
     5281 gttatgtcgg tgtatttgga aaagacatcc agaaaacagc caaaatgatg gtcaaacata
     5341 atcttgtgca gatggctgct tctgatgccc atggggtctc taagcgcacc ttttacttaa
     5401 aagagtgcta taagcagatt gccaaggact ttggtaagga aaaagtagta cagatgaaac
     5461 aagtcgcaaa ggatctgatc aacggggatg aaatcagtta tccagtctat gaagagataa
     5521 aaaagaagaa gtttggttta ttttaggggg agtgtaaggt gtttgtatta acaagaagaa
     5581 gaaaaattgc cacattggtt gttgtggaca gtttgttatt gatcgctgca aatattgcag
     5641 cagctaaatt catgaagccg tttgtagcta taccaatgga ccttatttta atctctaccg
     5701 gtttgtcgat tggtttttat ttattttatg gtagtttatt caaagttttc actcggatca
     5761 atcgctatac aaatttaaga gagatcattg caatttttgg ttcactatct gcaagtgacg
     5821 tctcaagtat cctcatactg ctgttcatta atcggcaata cagtttacgg ttagttatct
     5881 ttgcttatct gctttcgctc ttattgatca tcggtagccg attgatctgg cgtatctaca
     5941 tcgaaacaaa aaatatgaga tatgtatcag cggattctgc taaaaataca ttgatcgttg
     6001 gtgctggcga aggcgggcgt attttataca acagctttct gggatccaaa acggctcaag
     6061 atatccaagt tgtcggattt gtagatgatg atccgaataa aagaaatacg tatctttcag
     6121 gaaaaaaagt attaggtgca ttaaaagata ttccggaatt gattgaaaaa tatgacattc
     6181 aaatggtaac gatcgctatc ccttccttat caagaaaaaa attacgaaga atcttcgagt
     6241 tagtcgaatc cgctcacgtg aaagtcaaca cgatgccttc tatagaagaa cttgcttcag
     6301 gaaaaattag cgtatcaaaa ttaaaaacaa ttgacgtagt ggatttactg ggaagagatg
     6361 aggtaaagct ggatattgaa tccatcaaag atcagatcac agacaaagtc attcttgtca
     6421 ccggcgcagg aggatcgatc ggttcagaaa tctgtcggca aatcattcaa ttcaatcctg
     6481 caaagctatt gcttttagga catggggaga actcgatcta cttgattgac cgtgagttac
     6541 gcacgcatca tcaaaattgt cctacagaaa tcgtaccgat catcgcagat atccaagatc
     6601 gcgagaaaat caatgagatc atggaacaat atcatccaga tatcgtctac catgcagctg
     6661 ctcataaaca tgtaccactg atggaatata atccaaaaga agcagtcaaa aacaatattt
     6721 tcggtacaaa aaatgtagca gaagcagcta aagcagcaaa agtgaaaaac ttcgtcatgg
     6781 tatcgacaga taaagctaac aatcctccaa atgtcatggg ttcaacaaaa cgaatcgctg
     6841 aaatgatcgt gactggattg aatgaagaag gctgtacgaa attctcagct gtcagatttg
     6901 ggaatgtcct tggttctcgc ggtagtgtga ttccagtatt cagagaacaa attgctaaag
     6961 gtggtccaat cactgtaaca gactttcgta tgacccgata cttcatgacg attcctgaag
     7021 ctagtcgttt agttattcaa tctggcgcat tagcaaaagg tggcgaaatt tttattctag
     7081 atatgagtga accagtgaag attgtagacc tagccaaaaa catgatccga ttgagcgggt
     7141 atagcgaaga cgaaatcgaa atcattgaaa caggtatccg tccaggggaa aaactatatg
     7201 aagaactact gttggataag gaaagaaatg atgaagcagt ttatgagaaa atctttgtag
     7261 gaaacatcaa aggttactca atacaaaaag tgatggattt tgtcaagagc ctgccgcaag
     7321 acgatgaaca attggctaaa gatatcgtca cttttgcaaa tgcttcgaat aaataggggg
     7381 gaaatataaa tgaataaatt agaaaaacgt cagataccat tttcaccgcc agatattact
     7441 gatttagaaa taaaaaacgt tgtagaagta ttaaaatctg gatggataac tacgggacca
     7501 aagacaaaaa aattagaaaa aaaaattaca aagtggttag gaacttctaa agtagtttgt
     7561 ttaaattcac aaactgctgc tgcagaaaca gggttacgtt tgctgggaat aggagaaaat
     7621 gatgaggtta tcgtcccagc ttatacttat tctgccagtg catcggttgt atgccacata
     7681 ggcgcgaacc tagtattaat agactgtcaa caagattctt tggagatgga ttacgatgca
     7741 ttagaagcag caattacgga gaagacaaaa gtgattattc ctgttgatct tggtggtata
     7801 ccttgtgact acgaccgtat ttttgagatt gtggagaaaa aaagaaattt atttcaacct
     7861 agtaatgaga ttcagaaagc ttatggtcgt ataattgtta tggcagattc tgctcatgca
     7921 tttggagcta attggcatga tcagatggtt ggtaatgttg ctgacttgac aacattttca
     7981 tttcatgctg ttaagaattt tacaactgca gaaggtggag ctttagtatg gaaagagcac
     8041 gacaaactag ataatgaggc aatgtatcat cagtgccagc tgctttcttt gcatggacag
     8101 tcaaaagatg cacttactaa aacacagttg ggtgcatggg aatatgatat tgtgggtcca
     8161 tggtataaat gtaatatgac tgatgttact gcatcaattg ggttggcaca atttgaacgc
     8221 tatcctaata ttctgaaaag aagaaagaat ataattaaga ggtatgatga agcctttcaa
     8281 caactaggaa ttgaagttct tatgcattat acggatgatt atagttcttc aggccattta
     8341 tatattacta gagtacctaa cattagttct gagcaaagaa atgaaataat tataaaaatg
     8401 gcagaacaag ggattgcttg caacgttcac tataaaccac ttccaatgtt tacagcatat
     8461 aaaaatatgg ggtttgatat taataactat cctaatgcct atgctcattt tgtaaatgag
     8521 attacattac cattgcacac gaaacttaca gatgaagaag tagaatacgt aattaataac
     8581 ta
//
LOCUS       DACDIP010000088         8342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDIP010000088 DACDIP010000000
VERSION     DACDIP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.088"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(81..668)
                     /locus_tag="IV257_002490"
     CDS             complement(81..668)
                     /locus_tag="IV257_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815576.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            843..2165
                     /locus_tag="IV257_002491"
     CDS             843..2165
                     /locus_tag="IV257_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8815577.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2320..3096
                     /locus_tag="IV257_002492"
     CDS             2320..3096
                     /locus_tag="IV257_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8815578.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3089..3658
                     /gene="rnmV"
                     /locus_tag="IV257_002493"
     CDS             3089..3658
                     /gene="rnmV"
                     /locus_tag="IV257_002493"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8815579.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3651..4535
                     /gene="rsmA"
                     /locus_tag="IV257_002494"
     CDS             3651..4535
                     /gene="rsmA"
                     /locus_tag="IV257_002494"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8815580.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            4986..5615
                     /locus_tag="IV257_002495"
     CDS             4986..5615
                     /locus_tag="IV257_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815581.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5739..6848)
                     /gene="ugpC"
                     /locus_tag="IV257_002496"
     CDS             complement(5739..6848)
                     /gene="ugpC"
                     /locus_tag="IV257_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8815582.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7057..7479
                     /gene="mgsA"
                     /locus_tag="IV257_002497"
     CDS             7057..7479
                     /gene="mgsA"
                     /locus_tag="IV257_002497"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8815583.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7802..8338
                     /locus_tag="IV257_002498"
     CDS             7802..8338
                     /locus_tag="IV257_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8815584.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2631 a         1485 c         1730 g         2496 t
ORIGIN      
        1 gtgtgattga aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc
       61 tttattttgt aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa
      121 cggcttggta agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc
      181 gtacgtccga taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac
      241 cctaaaagca aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt
      301 gccatcactc caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc
      361 tttgtaaatg ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc
      421 ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg
      481 atcaatgcaa cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca
      541 acagtcatta atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct
      601 ttacgggaaa aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta
      661 aaatccataa actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa
      721 agaaatacat ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa
      781 attaaaatat tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg
      841 gaatgaaagg caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg
      901 gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt
      961 ctacaagttt gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat
     1021 tgacagtggc tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg
     1081 aagctgcgta tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact
     1141 ttcctgctaa tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac
     1201 aattatctac cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt
     1261 tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat
     1321 taataccaat tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc
     1381 agttatttcc aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt
     1441 tgttagcaac gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa
     1501 tgaaacggcc ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag
     1561 tcgtttatct attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg
     1621 gtaatttgaa tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc
     1681 tggtcactat tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg
     1741 ggatccgcgt gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga
     1801 caaagctttc aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag
     1861 cttgtattat gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga
     1921 tgtggctatt cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg
     1981 aattgccacg tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg
     2041 gtgctatttt cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta
     2101 taggtgttac cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag
     2161 agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc
     2221 tttttctatt tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt
     2281 gcttatgtgc taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc
     2341 catttaaatg ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa
     2401 atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg
     2461 caattaagcc atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct
     2521 ggcagttata cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt
     2581 gtagcattag gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa
     2641 gataaagtat ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt
     2701 catactagag aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat
     2761 atcggtggaa tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac
     2821 ttagggatgc atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa
     2881 gaggctgcag caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg
     2941 gcaccagttc cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa
     3001 aagattgctg aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat
     3061 gcgcaccgat tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt
     3121 cgttgaagga aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga
     3181 aacaataggg tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac
     3241 tcgtggagtg atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat
     3301 tatggaagct gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa
     3361 gaaaaaggga agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa
     3421 aaaggtcgtg acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct
     3481 tttagactat ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga
     3541 cgagttgcgc atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg
     3601 tattacgccg aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat
     3661 atcgtgaaat tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat
     3721 ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag
     3781 aaactgcgca aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat
     3841 tgacggaaca attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt
     3901 tgatccctgt attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag
     3961 atattttaaa gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt
     4021 taaaagtcgt agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg
     4081 aatcagattt ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta
     4141 tctctgctga accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata
     4201 tggaagccag tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag
     4261 attcagcgat tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa
     4321 aagcattttt ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata
     4381 acttgcagca ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg
     4441 aaacagctgg aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg
     4501 cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt
     4561 gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt
     4621 ttcgtttgtt tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg
     4681 ttgtgggcct gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat
     4741 gtttttgagt tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac
     4801 aaggagatgt gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc
     4861 ttatacttga tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag
     4921 ttattagtaa gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag
     4981 aaaaaatgag aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg
     5041 gggtcattat cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg
     5101 cgtctcatgt gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg
     5161 cgctaggtac gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat
     5221 tgtcacatct tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg
     5281 ttttaaaaaa tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga
     5341 tcggtgtgat tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta
     5401 atatgcctga aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga
     5461 ttggtgggct tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac
     5521 tcagcaaata ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt
     5581 cgattcaagg gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc
     5641 tcatctagta atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg
     5701 caccaacatc ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt
     5761 tctgtttctt tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg
     5821 cctggtttat ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt
     5881 gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg
     5941 gcttcaggtg aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg
     6001 aagataactt cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga
     6061 atacgaagct taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag
     6121 aagttcatgg caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa
     6181 acttctttcg gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca
     6241 gccatagtca ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc
     6301 tggtgaagtt tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat
     6361 aatggctcat ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca
     6421 cgttggcgtt gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct
     6481 aagatctctg ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat
     6541 ttcaagccaa aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg
     6601 aaaaccatgg caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata
     6661 tttagttctc cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt
     6721 ccacaaccgg aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa
     6781 tcagttacag aataattttc tgcattgtca tattttttat atacgttttt cagagccatt
     6841 tctaccataa tacgttccac actttctttt ttagttttat taactattta aagtataaat
     6901 gaaagcgtta actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat
     6961 gtgctagttt taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa
     7021 ataagtatgt taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat
     7081 gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa
     7141 catgaacttt ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg
     7201 atccatcggt ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg
     7261 gaggatcagt tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa
     7321 ccagatgtaa cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat
     7381 atcggaacag ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga
     7441 atcgtccatg aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat
     7501 aacgtttaat gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata
     7561 taatacgaac gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat
     7621 gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat
     7681 aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc
     7741 ggttttattt tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa
     7801 aatgaagaaa caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt
     7861 gatggtcact ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac
     7921 atttattcca caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc
     7981 agttttagct gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt
     8041 tacattgaat gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat
     8101 cacttggaaa aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt
     8161 gacaccatta tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc
     8221 gccccgcctg ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt
     8281 aggacgtagt attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa
     8341 aa
//
LOCUS       DACDIP010000089         8287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDIP010000089 DACDIP010000000
VERSION     DACDIP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.089"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            329..1252
                     /gene="truB"
                     /locus_tag="IV257_002499"
     CDS             329..1252
                     /gene="truB"
                     /locus_tag="IV257_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8815585.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1256..2197
                     /gene="ribF"
                     /locus_tag="IV257_002500"
     CDS             1256..2197
                     /gene="ribF"
                     /locus_tag="IV257_002500"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8815586.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2592..3377
                     /locus_tag="IV257_002501"
     CDS             2592..3377
                     /locus_tag="IV257_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8815587.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3390..4241
                     /locus_tag="IV257_002502"
     CDS             3390..4241
                     /locus_tag="IV257_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8815588.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4365..4817)
                     /locus_tag="IV257_002503"
     CDS             complement(4365..4817)
                     /locus_tag="IV257_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8815589.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5282..5650)
                     /locus_tag="IV257_002504"
     CDS             complement(5282..5650)
                     /locus_tag="IV257_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8815590.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5800..6282
                     /locus_tag="IV257_002505"
     CDS             5800..6282
                     /locus_tag="IV257_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8815591.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6319..7329
                     /locus_tag="IV257_002506"
     CDS             6319..7329
                     /locus_tag="IV257_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8815592.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7444..8157)
                     /locus_tag="IV257_002507"
     CDS             complement(7444..8157)
                     /locus_tag="IV257_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815593.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2812 a         1342 c         1680 g         2453 t
ORIGIN      
        1 attaagaaac tgaaaaaata aataaaaaaa aacggaaaaa agactggaag aatggacagt
       61 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
      121 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaaacata
      181 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
      241 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
      301 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttaactttat ggaaagaacg
      361 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
      421 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
      481 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
      541 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
      601 agatatgtct ttatctgaag aaaaaataga tgaagtgatg gcttcattta ttggtgagat
      661 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
      721 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
      781 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
      841 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
      901 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaaaga
      961 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
     1021 gctgccaata gaaaaaggag tgaaacgatt caaacgagta gatattactg atgaagtatg
     1081 gcagaaagta aaaaacggta tgagacttaa ttatactgta tttgcgctcc cagaaatgcc
     1141 gtcagaagaa attgctttgt tttataaagg caaagttgtc agtatctatc agccaaaccc
     1201 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
     1261 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
     1321 agtactagga ttttttgacg gcgtgcatat tggacaccag aaagtcataa agacaggtat
     1381 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
     1441 gattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
     1501 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
     1561 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
     1621 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
     1681 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
     1741 aggtgaaaaa atcagctcta cccgtattcg aggattacta gacagcggac aagtaaaaga
     1801 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
     1861 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cagttcattt
     1921 gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg gaaatttggt atccagccat
     1981 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagaattgta
     2041 tattttggac tttaatcaag atatctatgg tgaacatgtc gcagtacgct ggaatcaatt
     2101 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaagagga
     2161 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
     2221 tttatgtata actagcaatt tcaataaaat gggtgataaa taagcccagg aaaggggctg
     2281 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
     2341 tcggaaataa gccgaaattt cacaaaaatt tgagaagtaa tttttgtaaa ttacttctta
     2401 tttcttgaag ctggccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta
     2461 gcaattcatg aaaacgattg ctaaagcgta tcatttgctt tagactatat atcttggtgt
     2521 acactcactt taagacggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag
     2581 gagtgggtac tatgtataag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa
     2641 aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca
     2701 actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg
     2761 gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg
     2821 atacggcttt agaaaatttt gggacactgg acgtttggat taataatgct gggatggaaa
     2881 atcagcatcc aacccatgaa cttagccttg aagattggga aagagtatta aaagtcaatt
     2941 tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag aatgataaaa
     3001 aaggaaatat catcaatatg tcttctgttc atgaacaaat cccatggcca acgtttgctc
     3061 attacgctgc ttcaaaaggc ggagtaaaac tattcaccga aacggtagca atggagtacg
     3121 caccaaaggg aatcagaatc aatagtatag gacctggtgc tatccgtaca cctattaatg
     3181 cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac
     3241 gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca
     3301 gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc
     3361 aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag
     3421 ttgggtgggg gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga
     3481 tattaggaac tggtattgga gccgtgctcg ttggcttact tgttcaatta ttcgcttctc
     3541 cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca ttctgggtca
     3601 tcggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa
     3661 tttcgactgc attacagtta gtcggaacct cattgattgg tgtcttcgct tttggagaat
     3721 ggtctggaag tatgggaaaa atcattggtg cagcagcaat cattcttctt gtatttggtt
     3781 ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatca
     3841 gtctattagc atcaacaagt attggttatt gggtttacag tgcattgccc aaactagttg
     3901 atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga
     3961 tttatgtgat tttccgaaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacaa
     4021 tagttggagc catcttcagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg
     4081 gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta
     4141 tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat
     4201 tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatc
     4261 tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc
     4321 tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga tacaagtgaa
     4381 atatctgtct gcatcaataa tttttcattg atccattgac taaaatttac gacatgattg
     4441 aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga
     4501 cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggatttga
     4561 ttgattgctg taaaatcacg atactgaagg ttgaaacgca tcgtgattgg tgcatttagc
     4621 atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa
     4681 agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca
     4741 tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact
     4801 gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata
     4861 tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag
     4921 tcagaaaatt ctaacttttc tttctctaaa gaaattcgtt ctctcctaaa gtgatattat
     4981 atttaaatat acctgaaaat aatgggcagt attatgaaag atcactgata ttttctcgta
     5041 ttttttcctt attcaaaagt tttgcaggaa aattgctgaa aaaataattt tactaagtgg
     5101 aaaattcaaa gccattcaat cagtgatttt tttcagcaca agaaagactc cttataaaaa
     5161 tagcagatac tttgtcaatt aagtgtttaa ccagggatga taaaaaggat aggattgaac
     5221 aaaacctgta taagctgatg aatagtttcc tcgccagtaa aaggtttatt cgtataaagc
     5281 tttatcgcca aaatttatca tgaccttttt tatcttcaat attaaatgaa atcaattgtt
     5341 caatcaattc ccagttgatt acacttttcc aaggaacttt gatgacgtta tccgtatata
     5401 cgtagcctgc ttcttggata tttttttcaa gggcacgaat cgctgctttt tccggtgcga
     5461 tagaaaaatg atttttagca aaactaaatg caataataaa ggtaccatgg tcagtaaaca
     5521 taggttggtt ccattttaca gttgtgtcta gctggggata cgtttctgtt atcttagcaa
     5581 aaaggtcttt gatcttttgt tgatgttctt tttctttgat ctgattgatg atcggctgaa
     5641 atttttccat tctaaaacgc ctccattaag tggtttgatt tattttacca gaaaaacaat
     5701 agttatgtat aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata tttatatgat
     5761 aagatgtctc aaataataaa atagaaaaga gacataaaaa tgattgactt ggcaaagaaa
     5821 ttcttacacg aaaatatata ttttgagttg tatcatcatc gagccattta tacaaatgaa
     5881 gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa aagcttattc
     5941 ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa acaaacggat
     6001 ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg
     6061 gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga aaaacagatt
     6121 ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc accaggtaga
     6181 ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca gctacttgag
     6241 atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat
     6301 acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc aacgattggg
     6361 gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga atcagcttta
     6421 aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg gacgcaagat
     6481 aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat gatgcaagat
     6541 gaaaatatcc aaatgatctt tccgccttgg ggaggagaat tattgattga gattctcgat
     6601 tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga tattagtacg
     6661 ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac caaccttatt
     6721 gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca
     6781 agagaaggag aatcgattct acagcaacct tctgaaaagt atcaaaaaga gtggccccat
     6841 gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca atcagtcagc
     6901 ggagatgcag taaggatgaa gggacgatta ttaggtggct gtattgatac gatcagtcat
     6961 ttaatcggta ccccttatgg agatgtccaa tcttttaaaa gaaagtataa tgatggaaag
     7021 ccgatcttgt ggtttctaga aaattgtgag ctatcgtcag ttgatttaag aagatcactt
     7081 gttcagatga aactggcagg atggtttgat ggcagctcgg ggatcatgtt cggtagaagt
     7141 gcagcagagg atttgttaga ttataaaatg aaggatgtat atgaagatct gtcaaaggaa
     7201 ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca actgacattg
     7261 atcaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt tttgcaaacg
     7321 tttatttaaa aaattttttc ctgccttgga gtttatcaaa gaccaacaaa tctaaataga
     7381 aaagaaaatg aaagaagctg acagtatttt gccagcttct ttttgtatga tctattagaa
     7441 attttaatta tccataacta tcacattgat cagctgtttc acatcatttg gattacctgt
     7501 aggaatagct actacagatg cattatcaat aatttcttga gttgtttctc taatgaacgc
     7561 tggataagat gtttgatctg caggcatttg ccaatgtgaa ggaactggat aactagtgat
     7621 tgtcccgtca tggttacttg aatacactaa aactccctgg taactgtatt tcccagtgag
     7681 cataaccaca tctttcggat agactgcact ccctgcatca taaggggcga taggtgttcc
     7741 tgcaggataa tgaataacat gtagttcgaa atcacccgat gtatctctaa aagtattacc
     7801 tctaattgct agccaaacac gtgcgtactc aatttgtaga tcagaatatc ctgctaacgg
     7861 atctattgac tgagaagact gtgaggaagt tgtgtctatt gttgaagaag aaatatttgt
     7921 ctcaactgta ctactagatg tagaagaagt attgggctct gagactttac tgcttgttga
     7981 agatgtattc gtttcagtag aagctgattt ctgagtactt gatttttcag ctgaactggt
     8041 ttgtttgatt gaacttttgt ctgtggtcgc agatggatca ctattttctt tactaggaga
     8101 cgtacatcct acaaggaata aagagaccaa caataaaata aaacctaact ttttcatata
     8161 aaatacctca ttattttttt aataccagta tagcataatc ctgaattttt gaatccgtaa
     8221 aaccttgtct atggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag
     8281 aagaaaa
//
LOCUS       DACDIP010000090         8047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDIP010000090 DACDIP010000000
VERSION     DACDIP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.090"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV257_002508"
     CDS             complement(99..1229)
                     /locus_tag="IV257_002508"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8815594.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IV257_002509"
     CDS             complement(1878..3080)
                     /locus_tag="IV257_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8815595.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IV257_002510"
     CDS             complement(3140..3343)
                     /locus_tag="IV257_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8815596.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IV257_002511"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IV257_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IV257_002512"
     CDS             4880..5692
                     /locus_tag="IV257_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8815597.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IV257_002513"
     CDS             complement(6716..6868)
                     /locus_tag="IV257_002513"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815598.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IV257_002514"
     CDS             complement(7059..7937)
                     /locus_tag="IV257_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8815599.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
BASE COUNT         2458 a         1647 c         1254 g         2688 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagta
//
LOCUS       DACDIP010000091         7841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDIP010000091 DACDIP010000000
VERSION     DACDIP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.091"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(9..440)
                     /locus_tag="IV257_002515"
     CDS             complement(9..440)
                     /locus_tag="IV257_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8815600.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(458..778)
                     /locus_tag="IV257_002516"
     CDS             complement(458..778)
                     /locus_tag="IV257_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8815601.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1077..1853
                     /locus_tag="IV257_002517"
     CDS             1077..1853
                     /locus_tag="IV257_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8815602.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1868..2071
                     /locus_tag="IV257_002518"
     CDS             1868..2071
                     /locus_tag="IV257_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8815603.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            2087..2425
                     /locus_tag="IV257_002519"
     CDS             2087..2425
                     /locus_tag="IV257_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8815604.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2412..2591
                     /locus_tag="IV257_002520"
     CDS             2412..2591
                     /locus_tag="IV257_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815605.1"
                     /translation="MGKFNRALVFSAPLIIYALELWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2634..3104)
                     /locus_tag="IV257_002521"
     CDS             complement(2634..3104)
                     /locus_tag="IV257_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815606.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3191..3889
                     /locus_tag="IV257_002522"
     CDS             3191..3889
                     /locus_tag="IV257_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8815607.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4067..4408
                     /locus_tag="IV257_002523"
     CDS             4067..4408
                     /locus_tag="IV257_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815608.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4401..5072
                     /locus_tag="IV257_002524"
     CDS             4401..5072
                     /locus_tag="IV257_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8815609.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5078..5764
                     /locus_tag="IV257_002525"
     CDS             5078..5764
                     /locus_tag="IV257_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815610.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            5767..6516
                     /locus_tag="IV257_002526"
     CDS             5767..6516
                     /locus_tag="IV257_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8815611.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            6528..6797
                     /locus_tag="IV257_002527"
     CDS             6528..6797
                     /locus_tag="IV257_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815612.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            6959..7261
                     /locus_tag="IV257_002528"
     CDS             6959..7261
                     /locus_tag="IV257_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815613.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            7258..7419
                     /locus_tag="IV257_002529"
     CDS             7258..7419
                     /locus_tag="IV257_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8815614.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            7416..7721
                     /locus_tag="IV257_002530"
     CDS             7416..7721
                     /locus_tag="IV257_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815615.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            7721..>7841
                     /locus_tag="IV257_002531"
     CDS             7721..>7841
                     /locus_tag="IV257_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8815616.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETG"
BASE COUNT         2933 a         1219 c         1564 g         2125 t
ORIGIN      
        1 tgatgtattc atacatattg ccttgcgtaa gaatattttt tcttaaaata gcattggcag
       61 acagcataac aagtgtatct gagcttattc tgaatagaat aatactccat aaatttttcg
      121 agattaaact gagattcatc agtcagttcg ttctcaatat agatatttaa taaaattaga
      181 atagctattt tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac
      241 aaaactgaaa attcaaaatg agaggcgctg aaatgtgcaa gttcatgaga taaatgaaag
      301 gcttctgcag tttcaccgta tagattttca ttcaaaaaaa tgattctggg ttttggatag
      361 tagaaaccag gctctttcat ctccatataa actactttca aattgtattc gctcaacatt
      421 tctttcaatt tcaaatacat acaaaccatc actccaacta ttcattttcc tctaaagctt
      481 tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa
      541 aaacagtatc gtcttctgat aaatccacat gtttaggggt ttctctttct tctctaccta
      601 gaaggtagtc tacagagaca tcgaaatagt cagcaatccg acttaattca tctgaattag
      661 gagtattatt tttccacttt gctagataac cgttagaata tccaaaatta atttctaact
      721 gccgtataga tatctttttc ctcttcgcca agtcttttat tatttcatag gtattcatcg
      781 ataaaacaac ctttctaaat gtttacaaaa aaagtttaga aaaatacgca gaaataattt
      841 gactaattct gagtaatgag ctatactata tcttgtaaac agatttaaca actaaaaaga
      901 caacaaaaaa cactattgat atgtaaatgc agaccgccaa gaaagcttta aaatcaatgt
      961 ttttatgtct tatttaatta tgctcttagt atagagtatt actcagatac tgtcaattga
     1021 atttagaaaa aagttgttaa atttgtttac gaatataaaa gaaaggaatg agaaatatga
     1081 acacaccgca aatttttaat ttcgaacaaa acgaagttcg gacaatttta gtaaatgatg
     1141 aaccatattt tgtaggcaaa gacgttgcga gtgttttggg ttattcaaac actaaagatg
     1201 ctttgtcgcg gcatgtagat ttggaagata agatggggtc gcgaatcacg acctcaggtc
     1261 aatcaagaga gatgacaatc atcaacgaat ctggtttgta cagtttaatc ttaaaatcaa
     1321 aacttccctc tgccaaaaaa tttaaacgtt gggtaacaag tgaagtgttg ccagcaatta
     1381 gaaaacatgg aggttatcta actccagaaa aagtagaaga agctttgctt aatccagata
     1441 caatcattca attagcaact caactaaaag aagaaagaac tggaagatta atcgcagaac
     1501 aaaagattgc agagtacgaa cctaaaatct cctatttaga tagcatatta tcttctacag
     1561 attcagtaac aattagtcag attgcagcag attatgggat gtctccacaa cagatgaata
     1621 aattacttca taaactaggt attcagaaaa aagtcggtaa ccaatggtta ttgtgcaaaa
     1681 aacacatgaa ccaaggatac acaaaatctc atacaactga gatcccgaaa gccgatggtg
     1741 gcactaaaat tgtaatgaat accaaatgga cacagaaagg gcgtctattt atctacgaat
     1801 tactaaaaaa agaaggatat taccctcaaa tggatttaga ggaaattggt tagaaaggag
     1861 ttttagtatg actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata
     1921 tgtcgaaagt tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa
     1981 aagctactta tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat
     2041 agattcgatt atcactatgt acgaattgta aagggaggaa cagctaatgc aatatctaga
     2101 agcaaaaatc ccaattccag aaggctatgt aattatctcc caagtggatt atgaggagtt
     2161 aaaaaaagct gatgatactg gtagatggat gacgttgcca gaagtgctag aacggattaa
     2221 cagaaaatat gattggttta cttctagagt tttaaagaac ccaagatata gaaatattat
     2281 cgatatcgag aaaaacaaaa atggatttgt ctattatcca gttgaaggaa gagacacata
     2341 tctattttta agaagtaaaa cacttgaatt tttagaaaca aatttttcgg aaatcttaag
     2401 gaggcaagcg gatgggaaaa tttaatagag cgctagtgtt cagcgcaccg ctaatcatct
     2461 acgctttaga actttgggga agcaggcaag cgttgatagg aacgattgtt tacatggttt
     2521 ggatttttat ggggcttgat gaagctgaag ctgagtacag agcgaaaaag ccaaccgagg
     2581 gggctgacta aaaatgaata gaaaagagaa actagaatgt atattactat tactcagttt
     2641 aattctttca ctaatttctc tattgggaag tttgtatttt tgatatcaac aattttgtgg
     2701 atttgggttc tatttagagt aaatatcatt tttgtttggt gattaaggag tttattaaaa
     2761 ttaattttcc ctacttcaaa tgcaaggtaa caagatatag cacttttagg aggaatattt
     2821 agcggtacag cttcagagta tattctatta tcatcgactg atcctccttt agcaattaat
     2881 ttcttaaatg gagtagctga ccatctgcgg tttaagttag cggatgaaag ttctaaatta
     2941 actaacatag ctggttctgt tgaatagtta gaaatgatta cttttgtata aatcatatct
     3001 gcagcgaagt aagaagcatt aagttcaact tctagttgag ggcgcattcc ttttattttt
     3061 atccctgtta aaaacgtact caatataaaa cttattattg acatccattg aaaaattgtt
     3121 aagtgaaaaa aattcaaaat aaacaccacc agtttttaac taaattatac caaaaaggag
     3181 agaagaaata atgcaagaat tagtaatttt gaaaaataaa gaagctgtga ctacgagctt
     3241 acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt ttaagagata ttgatgcact
     3301 aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa ggtaatgaac cagattcata
     3361 tggcagaaat cgacgagttt tcttcattag tagagatgga tttttcttgc tggctatggg
     3421 gtttacagga aagaaagcta tctacttcaa acaaaaatac attgaagcat tcaacgaaat
     3481 ggaagatgtt attcgcaaga atactgttcc tcaaacaatt gaagatatga tgatctatca
     3541 attagaagaa atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat
     3601 tagcggacaa caagagtttg aaattaagca aaaaggaaat atgaaagtta tgggagttct
     3661 agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg
     3721 gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt
     3781 cgatgatgct gtttcattta ttgaaatgtg gttgccagaa actgcgatcc gtatggaaat
     3841 tgatcaactg aacagacaac agagactttt tggtgatgac aatgaataga gctgaagcgc
     3901 taagaatagg gacggcaatt gctaattgct ggtggaaata ctataaacca atcatcctaa
     3961 gccaacaaca tattgacaag caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc
     4021 aagcaaagag tcacaaagaa aacacatcat aaggagattt tagcatatgg aaaaagaact
     4081 ttccactcta gatcaatatt tgattgatcc tgattggggc aagccgaaaa ttgaggaaac
     4141 aagtggtcga aaaatcagac gaaatctttt gacgaatgaa gaactagctt gggatcaaga
     4201 tgatttaggc aaccatgtaa ctatttggga tcatgtttat cttatccatc tatcgaagca
     4261 ttcgaataaa cctgaatata tttacgtcat cgaagatggc ttgattgatg cgctagaaga
     4321 gtacgacaga gataacttga ttgatatctc ttattacgga ccaggtaaga aatacattgc
     4381 tgaaatggag gcagaatttg atgagtgaaa tcaaagggac aacgaacttt gaaaaacttt
     4441 ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa ttttgattat ttatcttggg
     4501 ctcacgcgtg ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt
     4561 acaaacacta cagggttgtt tctggaactc atcaagactt tcttgttgag gaatataaac
     4621 cttttcttat ggatgaaact gggacttatg tatctgtctc agtaacggtt aaaggacaca
     4681 cggaaaccga attatttcct gttttagatt atcgaaacca accagttgtt aaaccaaatg
     4741 caatgcaaat caataactca ttgaagcgat gctttgtgaa agcattggct ctacacggac
     4801 tgggattata tgtatttcaa ggggaagata ttccaacacc acctagaatt gatacaaaga
     4861 aattaaacat gctagagacg attctagaag ctttcaatga gcagatgggt aaagatatga
     4921 ccaaaacctt aatcgaatat gttaatgagc agacagataa attagggctc ttagctgata
     4981 acgttgaaac tattgaacag ttaagctatg agcaatgtgc cttgatggag cgagcaatag
     5041 cagctaagag aaaagaatta gataagaagt gatatgagtg tttaaaccat taatcgattc
     5101 atattcagcg gttctgaaaa agttcaaagg aaaagacata agcgcaacca tcaatgagga
     5161 agtgaacatt gatcgactaa agacgatgta tgacggctac gatggtgatc gagtcattga
     5221 aattcgtttt attgatccta gacgtttcac cgtacagcaa cgaaacttca tctatgcgct
     5281 gataggcgat atttttatcg atacaggcat gccaacggac ttctggaagg aattcttcta
     5341 ctttcgtttt gaaggtgtca cagggcgcaa aataagcctg aaagacgaat cgaatacaac
     5401 tgtgagtgat gccaatgtct tagcaaatat catcttagat ttcatctttg aacatcatat
     5461 tcctttcaaa gaaggctatg agattttacc tgcgaatcaa gaatattact tctacaaatg
     5521 catcacaaaa agagtctgct gcatctgtgg caaaacagga gctgacatcg atcactttga
     5581 caaagcgcta ggaagacgaa agcgcaaaga agttgatcat gcagagtaca catttgcagc
     5641 actctgcaga atccatcaca cagagaagca caaaataggt gtgattaatt tcaaaaataa
     5701 atatcaaatc aaagggatca aattaaacca gaaaacaatc aaaaagttaa atattggagg
     5761 gtaaaaatga cagaacatcg aagttattat gcgattatac cagccaacgt aaggtacgac
     5821 aaaagactta aaccaaatac taagttgtta tacggagaga taacggcctt gtgtaatgaa
     5881 agaggctttt gttgggcagg caatgagtac tttgcagatt tatatggtgt gaataaagag
     5941 accatatcgc gatgggtaag tgatttgatt aagtttggat acttgaatcg ggaaatcatt
     6001 tacaaagagg gtaccaatca aataatcaat aggtacctac gaattaatca ataccctatt
     6061 gacgaaaaac gcaatacccc tattgacgaa aaagtcaaag ataataatac atctattaat
     6121 aatacattta ataatacaaa agaatatata agagagttac cgccttcgaa aaaatcgaag
     6181 gctaagcccg tccgtcataa atacggagag tataaaaatg ttcttttgtc agatgagcag
     6241 atggagaaac tcaaaacaga attccctaat gattaccaag agcgaatcga acgactgtca
     6301 gagtattgtg aatcatctgg taagacttat aaaaactatt tggcaactat tcgaagttgg
     6361 gcaaggaaag aaaaaagtga acctaagaac gcaagcagtg gatacaagcg cacaggaaga
     6421 cgagagaagc ttcctgaatg ggcaatcgac caagaagcct atctcaagaa aaaagcgcta
     6481 gaacgagcta atagacaatc aaaagcacca ttctaagagg tggaaaaatg aagatcgatt
     6541 atctagaact aattaatgaa atagcgaatt ataaaaaggg cgaggaatta gacgtcctga
     6601 gagacgtata tgatcaactc gaagaagctg gaattgaagg aattaagaat gatcgttcga
     6661 gttggagtaa gctcagatac tatttcgcac tttatatcga tacaacacaa ttaagaaatt
     6721 tagcatatac caaattacta tttgttgatt gtgttaaagg cttgcaaaaa catcttaatg
     6781 aacttgagca ggtgtaatca gatggaccta aagacattta cagcacagat tgaactaatg
     6841 catcaagaag ctttaagaca aagtgtatcg tacgaagaca agtggctcaa cacgttccat
     6901 ggcggacgtg agagcgcact tgatcaagta ctcaaattac tgaaaggaga atgtcgggat
     6961 ggataagaaa gcggcaatgc agcgaattat cgaattgact tattcagaag attggcaaaa
     7021 tgacaaagaa gctgcttcag aagtgatgag gcttggaaga gcgatgtggg cagacaagag
     7081 caacaagcca agaccacgaa aaatcgcaat ttggcacggt gacaaacttc tagtgatagg
     7141 gacagctgaa cagttagcaa gtctcacagg cttgcacgag aaaatcgtga ggaaaagagc
     7201 aaggtgtggc tacacagacg ctaagaagag aacgtttaga tacgtggagg aatcatcatg
     7261 acaacagaag aagtgattca aatgcgtatt cgaagccttc agcgtgagat tgacgatctg
     7321 gaacgaacaa aggcagtgat ggtcaatgaa acggcgagaa aggcaatcga tttgcacata
     7381 gagaatttaa gaagggaaat ccatcgattg gaggaatgag cgtggataag aaagcagcaa
     7441 tgaaaagaat tgctgaatta accaagtcag aatcttggca agaagacaaa gaaatagttg
     7501 cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg agaacgccga
     7561 gaaaaattgc aatctggcat gatgaccgaa ttttagtaac aggtactgct gaacagttat
     7621 ctgaaattac tggattaagc aaaaacatta tctgggatag agctaggagc ttatggattg
     7681 attcaaaagg acgacagttt aggtatgtgg aggagagata atggatctca ttacacaata
     7741 cagtgacatc atcctcaaga aaatcatgat gaagattcag aaagataaaa aatcaaaaga
     7801 acgagctgaa ttagttaagt tagaaatggc tgaaacagga g
//
LOCUS       DACDIP010000092         7616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDIP010000092 DACDIP010000000
VERSION     DACDIP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.092"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..708)
                     /locus_tag="IV257_002532"
     CDS             complement(16..708)
                     /locus_tag="IV257_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8815617.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            793..969
                     /locus_tag="IV257_002533"
                     /pseudo
     CDS             793..969
                     /locus_tag="IV257_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1145..1714)
                     /locus_tag="IV257_002534"
     CDS             complement(1145..1714)
                     /locus_tag="IV257_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8815618.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="IV257_002535"
     CDS             complement(1788..2111)
                     /locus_tag="IV257_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815619.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="IV257_002536"
     CDS             complement(2265..3077)
                     /locus_tag="IV257_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815620.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="IV257_002537"
     CDS             3431..4279
                     /locus_tag="IV257_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8815621.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="IV257_002538"
     CDS             4424..5365
                     /locus_tag="IV257_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8815622.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="IV257_002539"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="IV257_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2270 a         1508 c         1371 g         2467 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 aacttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgagaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg aatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaac ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctcgaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttt
//
LOCUS       DACDIP010000093         7320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDIP010000093 DACDIP010000000
VERSION     DACDIP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.093"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            309..1691
                     /locus_tag="IV257_002540"
                     /pseudo
     CDS             309..1691
                     /locus_tag="IV257_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1927..2799)
                     /locus_tag="IV257_002541"
     CDS             complement(1927..2799)
                     /locus_tag="IV257_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8815623.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(2816..4288)
                     /locus_tag="IV257_002542"
     CDS             complement(2816..4288)
                     /locus_tag="IV257_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8815624.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            complement(4302..5150)
                     /locus_tag="IV257_002543"
     CDS             complement(4302..5150)
                     /locus_tag="IV257_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8815625.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            complement(5147..6013)
                     /locus_tag="IV257_002544"
     CDS             complement(5147..6013)
                     /locus_tag="IV257_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8815626.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            complement(6056..>7320)
                     /locus_tag="IV257_002545"
     CDS             complement(6056..>7320)
                     /locus_tag="IV257_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8815627.1"
                     /translation="GLAGCGSKEKSSGSSDPGEIKIWVQFSDETPEGKAWAEIVNNFN
                     DEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVITLDGPNTAAYAHSGVIAP
                     LDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYYNKSLFKDAGITEDQLPT
                     LEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAYTPFIWSNGGDVVNADGT
                     KAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKYAMMLSGSWTMADLEENY
                     QEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASAAFIKFATSTESSRIMSL
                     GNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVVAYPQVSRAFQQAVQDIS
                     YFEENPDVQKVADSRAAEMQQAIDTSLK"
BASE COUNT         2305 a         1547 c         1322 g         2146 t
ORIGIN      
        1 actatttgct aatagattaa aatttcagca tctatttctg accataaata aatgagctgc
       61 cgaaaagctg ccaaatcaga cttgaaaata aaggatcaaa aacttttaag tttctgaccc
      121 cgtaactcac acttcaaaat cttacttaaa aaggagtctg aaagaaaaaa agagtattca
      181 aaattttttt ctaactagct acatcacatc tatgcattga cactcaaaga accagaataa
      241 gtcatcagaa agcaaggggg tgcggacata atcctggacg aaaaaataaa cttaggagga
      301 atgtccgtgt gttttcccac aaagagcgtg taagggcaat tcagttattt ttaaaatacg
      361 attgctctta tgctgcaacc attcgagaat tagggtatcc gtcgattggt gcactacgga
      421 aatggtataa agagtatttg gaatccggag agctccatca ggagcaccga aaaaaatcaa
      481 agtattctga agaacaaaaa cgaatagcgg tgaatcatta ttttgaatat ggtcaatgtt
      541 atgcacgaac cattcgcatg cttggttatc ctaatagaga gtccttgcgg cagcggtgtg
      601 aagaacttgc accaggagct agaaaactcc gaaaaagtgc ggtaaagtta acacaagatc
      661 aaaaagaagc tgtgttgaaa aaattctata aaccgcaaac taataggaaa aatttagccg
      721 aatctggagg aattagtaga gttactctat atcaatggaa aaacacggtt cttggaaagg
      781 attttccatt gtgtatgact ttaaaaaatc aggaaaaaca aaaagagcta cttttaaagg
      841 aagtagaaga cttacaaaaa caagtacacc aattacagct cgaaaaagca ttactcgaag
      901 gagcggctga actgctaaaa aaagaaaaag gcgtcaatct tctttgtcta tcaaatcaag
      961 aaaaaacgat attgattgat gccttacgaa atcagttcac cttgaaagag cttcttcaac
     1021 agctccagtt tcctaaaagc agctattttt atcaaaaaca agcacttgag aaaccagata
     1081 agtattacaa agaacgtcaa attaattatc acgattttca atcataactt ctgtgcgtat
     1141 ggttaccgta gaattcacca agctttaaaa aatatgggta aaaagctttc tgaaaaagtt
     1201 gtacgtagat tgatgactga ggaaaatcta tttgttaaat tttctagacg aaaaaagtat
     1261 tcttcgtatg ctggggaaat ttctcctgca caccccaatt tattaaatcg gaattttaaa
     1321 gcaaagaagc caaatgaaaa atggttaaca gatattactg aatttagaat tctcgcaggg
     1381 aaaatctact tatcaccact tgtcgattgt tacgatggtg ctattgttag ttggactatt
     1441 ggaacaaagc caacttgctt cactctatgt ctaaaaagag ttattcacca gataattcag
     1501 cgtgcgaagg tttctttgga cgattaaaaa acgagatgtt ttatcaacgt gactggaaga
     1561 atacaacaat caatcagttt ataaacaaat tggatgatta tattcattgg tataataatc
     1621 aacgaattaa gttatcttta ggaaggctaa gccctcttca atatcggaag aagcaaagat
     1681 atatttgtta aaaaggtcca aaaaaaatat ccgcaccccc ttttttttgt aaaaaactat
     1741 tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc aagaacttat
     1801 ctcattcaag gcttgatctt ccaagtaaaa caaaaaccag ttaagcactc tgcctaactg
     1861 gttatgacta aattttcagt tgtatctttc tctaccaaca ctaaatgtcg aagagccttt
     1921 tttcgtttat gaatgtaaga ccgcttctcg agccaatgcc aaacaaccga gcgttccagc
     1981 gttgtcttct aatgctgggg tcacgatata tttgcttaac tcaggtgttt cgacataccc
     2041 attgaccaat tttgcaaatg cgtcgtggac gttttgcagc atatggcgtt gtttcatcac
     2101 accaccgcca aaaatgatca catctggaga aaacatcaag gttgtattgt aggcacattg
     2161 agcaatatag gaggcttcga tttcccaata aggatcatct tctgcaacct cttgtccttt
     2221 tttccctaag cgtttttcga tggcgggtcc agcggccatc ccttctaaac agttgccatg
     2281 aaatggacag ctgccagcaa agtcatcttt tggatgaggg acgacgagca tatgccccat
     2341 ttctggatga ctaaaccctt cgataaagcg cccttcttgt aaggcaccgc cgccaacacc
     2401 cgtaccgatc gtatagtaaa cgacactgct caatcctttg ccactgccga agacatactc
     2461 accataggca gccgcattga catctgttgt ccaagaaatc gggatcggaa attctttttt
     2521 catggtaccg atgaaatcaa aattttgcca tgccactttc ggggtcgagg taatgtaccc
     2581 ataagtggcg gaatcgcgat ggatatcgat aggcccaaat gagccaatcc caatccctgc
     2641 taaatctgct tggtattgtt tgaagaagtc aatgactaaa ggtattgttt cctcaggtgt
     2701 cgttgtcgga aagctcactc tttccttgat cgtcatttct tcatcgctaa tggcacacac
     2761 aaattttgtg ccgcctgctt cgattgaacc ataaatcatc actgagctct cccttctata
     2821 gattaattaa gccgctttcc aatgaaaagg ttacttcact gtctgccagc aaaagaaaat
     2881 cctgtccttt ttctgtctca taaaaagttg tactcatagt cagacgatca tttagaaaaa
     2941 gctcaatagc tgaagtatca cggaaaattt cgatggttag acaattattt tccaaaggaa
     3001 tcgtcacact gcgctttttc aacacttcct tttcagctcc tgataaatca tacggcatgt
     3061 gttccctgga agcagtaaag cactcccgtt caacatcata gttaagcagt aatgtatggt
     3121 cgggtccctt cgcatagtga agttctagtt tcgttgcctg ttttaaattg atttccaagc
     3181 gtaaatagtg ctggtcatat actaagtcct tcagtaagaa gcgattctct tcagtcttcg
     3241 cccattcaat cctttcctgc ggatcaatta cttgataaat tgatgaaact ggccgttgca
     3301 acaattgatt atcagccacc ctcagctctc taggcaaggt catggaacct gaccaaccat
     3361 ggcccagatc attcgtaggt attttacgct gccacatttg catccaagca atcattattc
     3421 tttggcctgc ttcgttttca caggtctggg gtgcataaaa atctaaaccg ccatcgattt
     3481 catgaaaatt ctctactaga aatttgcctg tttgccaatc gacagtaccg atgaaagcaa
     3541 cagttgagtt cgtattttgg taagcattgc cttgtggagt catttgaata ggagaaagaa
     3601 tcaagacttc tttgccatct agttcaaaca agtctgggca ttcccataca atgccttgtt
     3661 ctggagttcc ctccagcaat acggaataaa actcccaagt tagtaaatct ttggaatgga
     3721 acatcacaat ttgtccacga gcatctgcag ttttagtagc gactacagta aaatagtctt
     3781 cttttcgctg aaacactttg ggatcgcgga aatcttcggt cgttgcaaca gaagctaagt
     3841 gatcttctgc gatgattgga tttccggcat atttttcaaa gtgaatgcca tcttctgaaa
     3901 ctgccaaaca ctgaacttgt cgaacttgcc cttccacaac attgtgtcct gtataaatca
     3961 aatacagctt gccatccttt tctatggcac tgccggaaaa acagccttcc ttgtcatagc
     4021 tttcacctgg tgccagtgct actggcaaat gctcccagtg aattagatcc ttgcttttag
     4081 catggcccca atgcattggt ccccagatgc tgtcgtaagg ataatattga taaaacaagt
     4141 gatactcctc ttgataataa acaaaaccat ttggatcatt catccagcca atcggagcca
     4201 tcaaatgaaa tttattccgg tattcttgat tgactgtttg agcatttttc tcaataaact
     4261 cattggcctt taaaagtttt tgcaacattc actattcact cctattttat acccgttcca
     4321 gaaccgccaa gtccctgcag aatatatttt tgtgcaacga tataaacaat aatcagtgga
     4381 atcgttgcaa tggttaaaac agccatcact tgattgatat aaactggctg tgtagtattg
     4441 atcgtagtaa ttgccacttg cattgaaaat ttgctgctgt cagttaagac cattaacggc
     4501 caaatataat cattccagct ggagataaag gataagacac ctactgtggc tatggctggt
     4561 ttagacattg gcagcatgat gtgaaagaaa attttaacaa tatttgcacc atctaatttc
     4621 gctgattcaa tgatttcttc tggaatcgcg ataaagaaat ttctaaacag atagatatta
     4681 aacgcgctgg ccatcgctgg taaaatcact gccaagcgag tattcactaa gcctagtgaa
     4741 ttaacgatgg taaattgcgg aattaaaacc gtctccatcg ggacaatcag cagagctaac
     4801 aaagctccaa agataatttt cttgcctgaa aaattaacct tcgcaaaggc aaaacccgcc
     4861 aaagagttta caatgattga cccacaggca aaggttaaac cataaaaaat actgtttaat
     4921 aaataagttc ccacactgaa ccgggaaata acctcttgat aagttttaaa ccaattagct
     4981 ggattcaaac taggtaaaaa agctttgaag gaagctaaat tttgatagac atctgcttca
     5041 ggcttcatag aggaaactac catccacagc atcgggaaaa tgaataatgc cgctaaaagg
     5101 aacagcaaac aatattctac gatcattagc ggtccaaatt tcttggtcat gctggatcat
     5161 cctcctttac caattttctt tgagcaattg taattaaacc aatcatagtg gtaaagatta
     5221 acgcaatgga gctggagtag cctaccatac ggtctgtaaa tccagtttgg taaatgtaat
     5281 aaaccattgt catagtagaa ttcattggtc caccttgagt cattaccatt ggctgaatga
     5341 ttagtttaaa ggccgaaatc aatgtggtaa gtaaaataaa aatcgccgtg ggctttaata
     5401 atggcatggt gatatagcga aattgctgaa acttagaaaa accatcaatt tcggctgcct
     5461 cataaacgtc ttgcggaata ttttgcattc cccctaaaaa cagcagcatc tgatatcctg
     5521 caccttgcca tgccgatacg aaaacaagcg tatacattgc ttgtttggga ctagtcaaaa
     5581 aaggctgcga agcaattcca atctttgcaa atagcgtatt aaccagacct tcattaggat
     5641 ttaacaagta caaccataaa atcgaaataa cgactaagga catcacgact ggcgcaaaaa
     5701 aggctacttt aaaaaagata tttcctttac gatttttgtt gataataagt gccattccta
     5761 aagctgcccc tatttggacc ggaataatcc aaacaacaaa tttagccgtg ttccaaatac
     5821 tttttacaaa aattggatca gcgaacagtc gcttaaaatt ttctaagccg ataaattgtc
     5881 tagcatctgg cgttaacaaa taataatcag taaaggcata ataaattacc atgattacgg
     5941 gtatgatcag aaaaatcgtc agcagcagca aagctggcgc tagaaagccg taacctatca
     6001 aattgtcttt cattgtcggt ttttttaaca cttgattggc cccctttaga aacttctatt
     6061 ttagtgaagt atcaattgcc tgttgcattt cagcagcgcg actatctgct actttttgta
     6121 catctgggtt ttcctcgaag tagctaatat cttgaactgc ttgttggaat gctctagata
     6181 cttgcggata agctacaacc actgggcggg catgtccaga aagttggttt tgcttaatta
     6241 atacctgcat tccttccgac acttcatctt taattaaatc aactgttgaa tttctgatag
     6301 gtagaacact gttccctaaa ctcataatac gactagattc tgtactggtt gcaaacttaa
     6361 taaaggccgc agaagcatcc tttttctctg ttttctcagt cattgctaac tgccagcttc
     6421 cggttggtga aactaactcc ccagttgcat ctgaaacggg gtaaggcaaa ataccataat
     6481 caatctcttg atagttttcc tctaaatctg ccattgtcca agaaccgctc agcatcatgg
     6541 catatttgcc agtttcaaat cctttttcaa taggactgat tgtggtgtaa cctttttcaa
     6601 ccaattcttg aataaattcc aacgcttcaa cggatttttt accgttaaaa tagtcttcgg
     6661 ctttggttcc atcagcatta acgacatcgc cgccattcga ccatataaat ggtgtatatg
     6721 cgtaggtcag catttcgtct cccgacgcca tttgcatatc aattgcaggc gttttgtatt
     6781 tatcagtcag cttttggcaa attgcttgaa attccgtcca agtccaagga tcttctaacg
     6841 tcggcaattg atcttctgta attccagcat ctttgaacag cgacttattg taatagacac
     6901 ccacactgga ctctgagaaa ccaaacgcat acattttgcc atcgtaggtt ccttgctgaa
     6961 taatgctgtc taatacatca tccatgtccg catctttcaa ataatcatct aatggagcaa
     7021 tgactcctga atgagcataa gctgcagtat tcggtccatc taacgtaata acatcaggaa
     7081 gggtattggt tgtaattgca gcatttactt tatcctcata tccaccgccg cttccgctac
     7141 gaggaatata ctcagtaaca gctttatatt caccctcgta ttcatcatta aaattgttca
     7201 caatctctgc ccaagctttt ccttcaggcg tttcatcaga aaactgaacc caaatcttaa
     7261 tttctcctgg atcactgcta cctgaacttt tctccttact gccacatcct gcaagtcctc
//
LOCUS       DACDIP010000094         7028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDIP010000094 DACDIP010000000
VERSION     DACDIP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.094"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1180)
                     /gene="ltrA"
                     /locus_tag="IV257_002546"
     CDS             complement(<1..1180)
                     /gene="ltrA"
                     /locus_tag="IV257_002546"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8815628.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     K"
     gene            complement(1868..3562)
                     /locus_tag="IV257_002547"
     CDS             complement(1868..3562)
                     /locus_tag="IV257_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8815629.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            complement(3725..4318)
                     /locus_tag="IV257_002548"
     CDS             complement(3725..4318)
                     /locus_tag="IV257_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815630.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(4319..4651)
                     /locus_tag="IV257_002549"
     CDS             complement(4319..4651)
                     /locus_tag="IV257_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815631.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(4703..>7028)
                     /locus_tag="IV257_002550"
     CDS             complement(4703..>7028)
                     /locus_tag="IV257_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815632.1"
                     /translation="TIYQSFDLKDLLNAAGVNALFQSLISKVLALFCVVTLIYLGLKF
                     FMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAANKADKTGSPAF
                     QIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIITPEQAKGMAGND
                     KSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRYSSKPGIILA
                     GESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIEDISQSFLLL
                     AFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSVTKIFGVDTS
                     LKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGHRKDVTPDNG
                     EEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAEDLTEKAKDT
                     LEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQVNAAVKANTTTE
                     KENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPKTDPEQYRYKPN
                     SDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKRISDLPKRNQEV
                     QTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDNDQSFVHQSPAGT
                     VQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQNVHTGYKPANVA
                     FTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
BASE COUNT         1818 a         1495 c         1137 g         2578 t
ORIGIN      
        1 ctttgctatt aggacttttt tgaattcgtt tgatttgttt ttttagtttc actttaatca
       61 tgctttgtgc ttttgtacct atatgcgtat taacaacctt tttgttacct ttggtcacta
      121 atttcatcgt aaagcctaaa aattcacttt cttcttttga aagatttgtg atttttgact
      181 tttcttcaga tattggtaac tttagacgct cctgtaacca cattttagtt gcatagaaaa
      241 cttttttcgc ttcattataa tttctacaga aaattttaaa atcatctgcg tatctcacta
      301 ggtatatttc ttttagagtt gtactctttc tgagcttgtg atagatatta cctttatccc
      361 aagctccatt tttcctaaaa tgatgtttta gctctttggc atctttttcc tcccactgat
      421 ttgctatcca ccagtcaaat tcgttgagat tgatgttcgc aagtaatggg ctaagtattc
      481 cgccttgtgg tgttcctttt gttgggtata tcaacttccc atttggaagt actacgggtg
      541 ctcttaacat tttcctaatg attaagagaa gctgtttgtc tcgtataccc atagaccaga
      601 tttgtctcat gagtttagta tgattgactt cgtcgaagaa acctttgata tctacatcta
      661 ccacatattc catgtgactt tgattcattc gggccataca atctgctatt gcgtgttctg
      721 ctgatctatt tgggcggaaa ccatagctgt gattattgaa ttttgcttca catattggtt
      781 ctaatacttg taagatgcat tgctgcgcga ttctatccca tattgatgga atacccaacg
      841 gtcttaattt accattatat ttaggaattt ctacacgcct tactttccga gggttataat
      901 gtgcgaatcg cttcctaaca atttctacga attcattacg gttgagattt tcaatatctt
      961 taatcgttac tccatcttct cctcttgtca cactaccgtg atttctcttg atattacgat
     1021 atgctaggac aatgttgtta tcagaaatga taatctgcat tagattcttg aaattatctc
     1081 ccttcaaact tttggagtat atcttatcga agatttccgt catgccataa tattctgcat
     1141 gccgcaactt cctatctttt ggacttgttt cttttgtcat ggtttatcac gctccaattg
     1201 gctcgtgacc tttttagtca tacgagaatc ccatggttga acgggtttca tgacttttac
     1261 agacgaatcg agcctgttcc tccagttcca ttacagaacc ttcatcagtc gtgactctac
     1321 tttcaccatc actaaagtaa gttatttgtc attcacatat ttcttactac cacttcatcg
     1381 agtatgcact ctccatgttg atggcttccc ctgttccctt atatctatcc acatgcctta
     1441 acttagatgc tagcttttgc cccctaactt ctcctacgcc tgtaacgcag catggtcttt
     1501 cataacaacg acttttactc aaccacaagt tagcacaatg agtgattttt tgctcactgt
     1561 ctctcccatt tctaggagct atgcctttcg gacagtacat tcgctagttc gtcagtacgt
     1621 ttgtacatac tcaccatatt aagttttttt ggacacccga cttatcggat acacccttta
     1681 cctctagaga actttcgtgg tattcactat ttcttttacc ttacggtatc cttcagccga
     1741 cttcaccgag cttcacacaa cgttggtctg cataaactac ttgttgcatg tcggagtatc
     1801 agtggaagcc ctttgtggcg ttacccactc atttgacttc tcgttataag agttattaca
     1861 atagttttta gactacttgc aacccacagc ttttcactgc agaacaggtc gcaccattat
     1921 ggcactcatc aatcatcgcc aagaagcgtt tgaagtcttg ttcttccatt tgtttttctt
     1981 taattaaacg tcgttgtctt cttccttgaa tcaatgcctg attccaaatc aacgtcatag
     2041 ccatgtacat ttgacattgt tgtacccctc gatctaagcc tgtaatagag tcaatgtcaa
     2101 aaaagaccaa ctgttgttgg tcaaggtgtc gaatcgtact atgaccatca aaaatcatgc
     2161 catacgtctc acataaggat tctacgatgg attccatttc ttcttttcga ttctttttct
     2221 cttgggaagc acctggtaat tccagttgtc ctaaaaagtc taaaaattct ttgaacgtcg
     2281 gataagcttc tggatctttc cctgtgactt gcgtatcagt atttgaacgc gaatttgagg
     2341 aagtaaagcc gcgtggtact gttccatact cttcataaaa acgatttaag taggtcttaa
     2401 attctgcttt catcgttcct tttagctgag gactcactaa ctgtacctga tagaccactt
     2461 tgtctaagtg tgtttgatag gacgctgctt cattgatctt caaatctttg actgatccat
     2521 cgtcatataa actggatgct tcatcatacg tccgtgtggc aaatatctct aaaggattga
     2581 tcattccgtc agatccgtct aaacgcacca taataccgtt atattccttt aagaaaggaa
     2641 taaagtcttt tcccttttca aagccccaaa taaagtctcc tgcagctaaa tgggcttccg
     2701 cgatcatttt aagaacggtc gatttaccag ctcccatcat tcctagaagc aaggtattag
     2761 tagacaaccg tacactattt ttatacgtcg cattccagac aaatgggccg cctgtcatgg
     2821 tttccccata ttgtgtccct cctggatctt ttagcgtttg gtggtggaac gggaaacctc
     2881 cgccaatgtt taacgatggt agtgattgcc ctttgatatt tccgaaccat tcagcttgtt
     2941 catcgaacat ggtaaacatc gattgccaat ctcgtttggt tttatgcaga taagtcaccg
     3001 cacgataatc catccctttg agattttcga tcagttcctg cgctcgttca tctacttctt
     3061 gaatcgtgtt tccgtaaaca tgtactctca ctttgacttg cttagaaatt tccccattag
     3121 aagtcaattt tctagcatat tccgctagtt ccaggtattc ttccgcgctt ttatttcggt
     3181 cagtcgcttt tcgttcatta tctgatcgat catttaattc atctaaggca cgattgattt
     3241 gatcaatcac tttttgcttt tcatctgtgc taacatcgag tgtggccact gtatgaggat
     3301 tgttcatgag attggccaac caaaaaagcg taggattttt tgtgtagtga taaactgtca
     3361 cacatcgtga ataaccatta ccatacgtca catattcttc atcaaaattt aacccacctt
     3421 gtggcatcac ttgttttaaa aaatctaaat catacccttt ggattttaca atttctattt
     3481 cccgatttcc tagttgcttt aatggatcaa gtactttttg atcattttgt ggtgaaaaag
     3541 ctgttttaat tgcttctaac atgtacagtc acctcttctt gtttgattct ttacttggtt
     3601 aaaagattgc ccttttccca acttacccag gcttaaagtg tgaggaaatt ttctagtggt
     3661 ctcttccttc ggaacatctt tttctaacca tatcaacgga tatttcattc tctcttctcc
     3721 attcctactc atcattcata ttgttcattc gatacagaat cttgatcaat tcttttttgt
     3781 agagtggttt taaccctaaa atgccactag atcgcattaa ctgacggttt ctggtttgta
     3841 aatccttgat tgtctttcct tcaatgacga aaaagaagct taagtcagga cgttcttgtt
     3901 ctgctcgttt caattcctgg atttcttcga ctaatttctt ttcatatttt tttaatcgtt
     3961 gtagatttct tcctaaggac tgttccatac gatttttatc taataattgc cgcttttgca
     4021 cgatttgttc cgttagatcc gtctctgtgg tcaaactaac taacttaatt gttggcacat
     4081 acgtacgcat gacggtggtc aactgattca ttcgttctag tttctcttgt tcatttaatc
     4141 cttccacgtc tgtagaaact acacgataat aacgttgaaa atgatcttct ctagtaatta
     4201 agactccctc ttctgtacag gatttatatg gaaataagtc caagatagag cgctcttctg
     4261 gttttccaaa ctgcaaaatg gatttccttt caactgaatt ttgtcctttg actcgcattt
     4321 attcatcctc cttatacgta ccatgataat ttcggttagg atctaacgca tgataatggt
     4381 tcttgtccgc aaaaaaaata gactcgataa tcgcaaaaat ttgtcgttga cctgggttat
     4441 tatagggtcg ccaaacaatc actaacgcta tcgcaatcca taagccataa ttcaagagtg
     4501 caaaaaagag tggtaagtgg acaagattat tgaccatttt cgcccaaaaa aagcagccac
     4561 caagcgttaa tagatctaaa aagaaaaaag cgaaaaactt ccattctcca ctaaatcttg
     4621 gagggttttt catgttacta tatttatcca taaatgattt ctccttttca tacatagaaa
     4681 aaaggcctaa cttgtaggcc ttttatggtt tgatatcctg tttgatatcc ctcaatagtt
     4741 gttctttcaa tgcattgttc ctgttttttg tttctcttgt cacatttatt tttgttggtg
     4801 cagtaaatgc cacatttgct ggcttgtaac ctgtatgaac gttttgaata ggttttggtg
     4861 cagcttctct tgtcacaaca ttttgaacgg acgcattccc tgtctcacca ctttcagata
     4921 aattgtaaga aacagttgtc agatttccat tttcaggcaa acgacttggt ttcatttgaa
     4981 cagtacctgc aggtgattga tgaacaaaac tttgatcatt atccttgaat tgattcaaac
     5041 gactatcttt cattggtaaa ttttgtgcct taggtaaacc aattttcgtt gatttttcag
     5101 ttccaacttc gattccatta tcatttggca gaacgatgga atcctcatcc gtttgtactt
     5161 cttgatttcg ttttggtaaa tctgaaatgc gtttttcatt ttcggagtta acagatctat
     5221 accgatattg ttctggatca gtttttggta actgtccctc attttctgct ttgtcttctt
     5281 gatttacttt tgataaatct gaaattcgct tctcattttc aaggtcactg ttaggtttat
     5341 agcgatattg ttctggatca gtttttggta actgtccatt ttttccttct tctgaatttc
     5401 tttcttctat ggaatttttc tcttcaatcg aattttcatt agagtgcaac gcttcttggt
     5461 gatctttgac tgaaggaatc actatacctt cttgattttc tttttcggtt gttgtattag
     5521 ctttcactgc tgcattgact tgtccttcac catacgcatc ttttagtcct tctttgactt
     5581 ctgaagcttt ttctttttgt ttttctagtg cttcggatgg attcttcaca gcttcagcca
     5641 attctttagc tctcgtgccg tctaatttat cttctaaggt gtctttggct ttttctgtaa
     5701 gatcttcagc tgttcctttt aacccacgtt cattgacgta accaagacct tctgcggctt
     5761 tagaaacacc atttttcgct ttcccgaaca tttcggaaac tgttttctta ctattaggta
     5821 cagttgtagc tttatcgtct gattgttctt ccccgttatc aggggtaaca tcttttcgat
     5881 ggccaaatcc atttttacga atatcattta aatggcttaa accactctta ccagcacttg
     5941 caaggttttt agctccgcta cccacatttt tcgcaatatt tcctaaggca aacatgctaa
     6001 gaagagaatt actgccgttt ttcaggcttg tatcaacgcc gaaaattttc gtaactgatt
     6061 gagagccttt gaatagcgca atggttagta caatcaaagc aacgacatac aagaatccat
     6121 tgatctttcc tgccgcatgc aaatcaccga tacctgaaag cattaaggta tagattcgta
     6181 attctaaaat cgtaaacgcc aataacaaga aggattgaga aatatcttct atcgcacttt
     6241 tcatccgttg acctgtctca aagtccgttg acgcggcaat cactaaatag aattttttaa
     6301 ataccagctc aataatgcaa gtaacgatcg taaataggat aaataaatag gctactgcca
     6361 aagaagattc accagctaaa ataatacctg gtttactact gtatcgacga taccctgaag
     6421 tatagacata ttttgctaag ccttcgtcac taatttgaac aggaatttct ttcccattat
     6481 catctagttc caatcggtat tgtaatgcct ttaaccgatc cttttgtata tctgataacc
     6541 cacttttatc attacctgcc atacctttag cttgttcagg agtaataatc ccattgatat
     6601 tcgctgtttt aaaatttttc gctgttaaag catttctttt atctgttggt agggattcta
     6661 ctttattcgg gtcagttgct agtaacgtac tgacatcgat cacattattt tgaataattt
     6721 gaaaagcagg tgatcccgtt ttatctgctt tatttgctgc agtgacgtct ccgtaaaagc
     6781 ttttactgat atttaaccct tcatcgatta aagaactacc accaaaaatc aagatcatgg
     6841 ccataaataa attaaccaag atatttttga cttttggtgg gtgtttactc ataaaaaatt
     6901 tcaagcctaa ataaattaat gtgacgacac aaaaaagagc caatacctta ctaatcaaac
     6961 tttgaaatag tgcatttacc ccagcggcgt tcaataagtc ttttaaatca aagctttgat
     7021 aaatggtt
//
LOCUS       DACDIP010000095         6851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDIP010000095 DACDIP010000000
VERSION     DACDIP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.095"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            238..1629
                     /locus_tag="IV257_002551"
     CDS             238..1629
                     /locus_tag="IV257_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8815633.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1688..2491
                     /locus_tag="IV257_002552"
     CDS             1688..2491
                     /locus_tag="IV257_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8815634.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2524..3378
                     /locus_tag="IV257_002553"
     CDS             2524..3378
                     /locus_tag="IV257_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAP8815635.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3375..4076
                     /locus_tag="IV257_002554"
     CDS             3375..4076
                     /locus_tag="IV257_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAP8815636.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4084..5151
                     /locus_tag="IV257_002555"
     CDS             4084..5151
                     /locus_tag="IV257_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8815637.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5144..6109
                     /locus_tag="IV257_002556"
     CDS             5144..6109
                     /locus_tag="IV257_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8815638.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6154..>6851
                     /locus_tag="IV257_002557"
     CDS             6154..>6851
                     /locus_tag="IV257_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8815639.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKW"
BASE COUNT         2475 a          952 c         1315 g         2109 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga
     1561 ctagatgaag atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt
     1621 tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa
     1681 tgtaagtatg ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt
     1741 aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc
     1801 tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga
     1921 acatacgaaa aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg
     1981 attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa
     2041 tattgaggaa tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata
     2101 taacaatgca attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat
     2161 gtttgataaa aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga
     2221 ttattatctt tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac
     2281 attagtctat gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct
     2341 tggttctggt cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga
     2401 ttttataatt gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg
     2461 aaaattttat caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca
     2521 aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt
     2581 ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa
     2641 caagggttta ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa
     2701 aaattttatc aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct
     2761 tctaaaaatt ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt
     2821 attaaaactt atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg
     2881 ttagatgttg cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta
     2941 tatgctttat tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt
     3001 agtaagatac tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt
     3061 gctgaaaaac aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt
     3121 gtcggaccta aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg
     3181 gtgccattcg aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa
     3241 gtttttggtg actatatgca gcttccgcct gaaaaagatc aggtttctca tcatgaagca
     3301 gtttatattg atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa
     3361 ggagcagaaa aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg
     3421 gacaagatat cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt
     3481 tagaatcatt tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt
     3541 gggaagaaac gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca
     3601 ttacaggtgg atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact
     3661 attctgaaga tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac
     3721 tggtattgtc agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac
     3781 cttacaacga acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc
     3841 gtaacacatt gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag
     3901 catatgaccg ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca
     3961 tgatggtaga tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat
     4021 tgacaacaac agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga
     4081 aagatgatga cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat
     4141 catacaccaa aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga
     4201 atgatcggct cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa
     4261 atcaaagttt atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat
     4321 cttgaattag atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa
     4381 attcaagctg actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact
     4441 gatccaatcg gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca
     4501 gtagcaaccc aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat
     4561 cgaggcgata tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg
     4621 agagcaggct accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca
     4681 aaacacgacc tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt
     4741 ttatctgata cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt
     4801 gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct
     4861 gctattcttc atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa
     4921 aactttgatt tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa
     4981 gtaatttttg aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca
     5041 gtacttgaaa ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa
     5101 tcattggttc atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat
     5161 tattgttcct gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc
     5221 ccaaacattt actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg
     5281 gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc ataaagaaaa
     5341 cggcggatta agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg
     5401 ctttgttgac agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt
     5461 gaaaattcat gatgccgaca ttagctctgt tgaattgatt cctttttatg gtgatagata
     5521 taagaaagct aataaagaaa agaaagtaat tatattaaat aaaaaggaag caataaaatc
     5581 tgttttagaa ggaacgcaat tttatgcata cgcatggaat aaactttatc gtaaggaact
     5641 ttttaaagat aatcgttact tggatggaaa aacatttgag gatgcatata taataataga
     5701 tctattattt caaacagaaa aaattgttgt ttccaatgaa gagaaatatt tctatcttca
     5761 aagaaatgat agtattatgg gaaaaagttt ttctatgaat aatttcgatg ttatagaagc
     5821 atggcaatat aacaaagaga agattttaga tgcttttcca gatttgcatg attcctatta
     5881 tcgccgacta tgttgggcat atttttacgt gttggataaa atggttttat caagtagtta
     5941 tcaacaaatt ccacgtaaaa aaacagtaat tgaattttta aagaagaaca gaaattttat
     6001 attaacttat aagggattca ctaaatcgcg caaaatagct attattgctc tgagctttag
     6061 agtagattgg tataggaaat tggttgaaca aaaaaataaa aaaaattaga atgtttaaat
     6121 tagtaaataa aagaaaagga attgaaaact aaaatgtgca gaataagtat aatagttcca
     6181 gtttataatg ttgaaaaata tttaaaaaag tgtgtcgatt caattctagg ccaaacattt
     6241 accgattttg aattattatt aattgatgat ggttctacag ataattcagg aagtatatgc
     6301 gatgaacttg cgaaaacaga caaccgaata aaagtgatac ataaggaaaa tgggggtcta
     6361 agtgatgcca gaaatattgg tattgaggta gcaaagggag attttattgg gtttatagat
     6421 agtgatgatt acattgatga agatatgtat gcatttttat ataacaatat attgaaatat
     6481 gatgcagagt tatcaatgtg tggtatttat gacgtccata agaataaaga aattaaaaag
     6541 ttaactcctt tttctcaatt ggttactaaa tctgaagcaa ttgaattagt attagatggt
     6601 aagctagttg ttgctaatgc agtaagcaag ctatataaaa aagaattatt tgagaatgtg
     6661 agatatccaa aaggaaaaat tgctgaagac gcagcagtaa tactgaaaat tatcaatcag
     6721 tgtaataaaa tccatgttga tacttctcaa aaatattatt attatcatcg tggagatagt
     6781 attactagta aaaaatttac aaaaaaagat tttgatataa ttgatgtatg gaatgagaat
     6841 gaaaaatgga t
//
LOCUS       DACDIP010000096         6657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDIP010000096 DACDIP010000000
VERSION     DACDIP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.096"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2693)
                     /locus_tag="IV257_002558"
     CDS             complement(<1..2693)
                     /locus_tag="IV257_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8815640.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDS"
     gene            complement(2707..3924)
                     /locus_tag="IV257_002559"
     CDS             complement(2707..3924)
                     /locus_tag="IV257_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8815641.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(3914..5509)
                     /locus_tag="IV257_002560"
     CDS             complement(3914..5509)
                     /locus_tag="IV257_002560"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8815642.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            5634..6419
                     /locus_tag="IV257_002561"
     CDS             5634..6419
                     /locus_tag="IV257_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8815643.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1941 a         1355 c         1115 g         2246 t
ORIGIN      
        1 gaatcaatat catacaactt agtggtattt tcactataaa attcgatcgt tgaaaaatct
       61 tctccctctt tttctggttc ttcctccatc aatgaaccct tcacggtctc gtacacgcca
      121 atttgttctt ccaaaagttg aatttgattg ttcaaagttg aagattgcac catgtcatca
      181 ttgtaatcat cataagtcac taacgattca tacgcacgat tcaattcttg aaatgctttg
      241 acgtactcga tacggatttc aagtggcgta tgttcatcaa tggattccat ggatgaaacc
      301 tcttttaact taccatatgc ttgcttgaat ctttttttgg attcttggta acttggataa
      361 atcaaacccg attcttcttt agcttctgaa tacagttttg tcgcatcctc gacattcttc
      421 tccattgtcg ctggctttcg gaaagtaaca attagtcctt ttgttttttc aggatacgta
      481 cggttcgtac gtgaaaaggc ttggatcaat cctgcatact ctagattacg atccacaaat
      541 agggtttgaa tcgtaggggc gtcaaaacct gtcaatagac gatccactac gattactaaa
      601 tcgatttgtt tcccaaattg tttgaattcc gctctttttc gtgccaaacg attattgatg
      661 tctccgttat aacgctcaat gtctgctagt gaccaggcag tatcataata ctgattatat
      721 tcctcaataa ttttttgcat ttcttcttgt tgggcacttg aatctcggct attttcttct
      781 aatgaatagg taatagcgat tcgtgggaaa tcaggatcct ccatcgttcg tccttcacgg
      841 ataggctgat tgggaaactc ttttattaac caatcagatt ctttcgtcat ctctttgatg
      901 gcccgataat actttttggc catatcaatg gaactcgtgg ttaagatcgc agacttcgtt
      961 ggacggccat ttcgaaaatc aaattttgta taggcattgt ctggacggaa cattttatga
     1021 atgacttttt ggatatgttc ctctgcttca aaaacagtag ggtccaaata actttctttt
     1081 ttgacagact ccatttgatc gatcatacga ttgatctgtt ccggtgaata ctctgcatat
     1141 gtttctactt cttttaattt ccgatagatt ttattctcta atgaagtcgg ctccacggtg
     1201 ttttcatgct ctacttgaaa tcctaaaacc gagccatctt ctaatgcatt cttgatggta
     1261 taggtgtgca agacctctcc atactgatca tgggtcgtac gtgccaattg ccctttggct
     1321 tgtttccgat tctcagggaa aatcggtgta cctgtaaagc caaaccaagt ggatttcgga
     1381 aacatttttt tgatttcttc catattttca gcacttaacg ctcgatgaca ctcatcaact
     1441 acaaacacga tatgttgccc cattaatttt tggaagcgat tggttccttt tttctcttcc
     1501 tgtttctttg catatttcaa cgccgcatct agtttttgac gggttgtaat gatcacaaca
     1561 ttcgcattgg catctgccaa cagagtttca cttaactctt tggcacttcc tgttcccacg
     1621 atcaagctat tcgctttggc attaccagaa gaaaccccag tattaaactc cgaagcaaat
     1681 ttcgtaaact ctgtggtggt ctgattatct aaatctttgc ggtccactaa catgatcgtt
     1741 cgatcgatcc ccgattttcg tgctaaaagc ttagtagata caaaactagt caacgtcttt
     1801 cctgaaccag tcgcatgcca cacataccct gattgatgtt tatttgcggc aataaacaag
     1861 gcttgaatcg catgcacttg ataaggatgt aacaccatga gggttttatt gtcttgatct
     1921 tcactgacaa tcgtataatc cgcaatcaag cgatgggcat ctgggatatt tagcacttgt
     1981 ttgcaaaact catataagtt ttctaccttt ttattatctt tcgttcgcca actaaataaa
     2041 aacttaggat gcaaatcctt cggtaaggca tttgcaaaat accgagtcgt ttgttcattg
     2101 gaaaccacaa acaactgtaa ggtcgaaaag atattgttcc gaaacatgcc ttcttcagca
     2161 tattttttga tttgattaaa cgcctgataa aaaccatctt ttgcagttac ttgttttagc
     2221 tcgatttgga taatcggtaa gccattgatc aataaggtga cgtcaaaacg tcgatcccgc
     2281 gcttctatgt tacttccacg ttttgcaatt tgatggacca cttcataagt agagattcct
     2341 ccaccaatgt cttgattgga ataaaggata agtgagacag aacccaattg cggattttcc
     2401 cgttcaatcg tgatccgagc catcccattt tcacctttta accatttcgc agcatcaaat
     2461 ggggttttcg tccgtagcaa taattcggtt ttgatcgttt caaactcttt atcggtcaaa
     2521 ggagaatcat tcagttctgc ttgattattt gagatgattt tttgacgtaa attgctccaa
     2581 agatcttctt ctgattttag atccggacga tacgtccact gattatgtcc ttccccgagg
     2641 acacgaatca actgttcttc gacttccgct tcctcacggt gactaatttt cattcttctt
     2701 ccctccctaa acaaacatct tttgcaagaa gccttttttc gtttccttca gcaaatctaa
     2761 cttacgttga tgaagagcga tcgtatcatc tagctgcttg aagaaggcac caattttttg
     2821 ttgttcattt atatccggtg gaaactgaaa tgtcctttgt tctataccac ctttagtcac
     2881 atgtaccatt gtcgttccgt tagttgattc tttaattttc tctttatcag tcaatagcca
     2941 agtatataga tactgtttgt ccaattcctt agataaaaat ttaagtttcc aaatatgata
     3001 atggtagatt acacgttctt cattccacat ttcaggaccg aagcttgttg cccataaata
     3061 taagagatct cctttattag catacttgtc ttcctctaat tctaagtttg aataatacca
     3121 tctatcatta gtattaaaat tacctactct taaaacccta tattttccag aatcaagtaa
     3181 ttcttgttgt ttaaaagctc taccattcaa aaaaactacc acttccccca acttccgctc
     3241 ttcccaatct cccgtaaatc ctggaaaacg aacttctggc acttttgctc cgtttttagg
     3301 gaacattttt tgtaagaatc cttttttcgt ttccttcagc aaatctaatt cgtgctggtg
     3361 aagcatgatg gtgtgatcaa gttgtttgaa aaatgaaccg atcttgatct gttcgtcata
     3421 agttggaagt gagataggtg tctttttacc actatcaatt gtataggttc cgacggttcc
     3481 aatgcctgca acgtcacgga tatacttctg aattttgtta tttatgaacg agtagtaaaa
     3541 gaagtttccg tcaattttat gctcattctt gaaccaaatg atgtttccat ccttaaagta
     3601 aattgggtta tcatcagtga ttaataaggg gacacctatt gatccgacac cagttacaag
     3661 caagtctcct tgagaaactt tacctgaatt tttcgaatat tcattatatt tttcttctga
     3721 aatgtaaaga taatcggatg gttcctcatt ttttgcagct gagacaatat cacgagcacg
     3781 taaaaatcta atacctgagt tagtccaatc tgattgatga attcgcttca cagaagtaac
     3841 atccatcata tctcctaact tacgctcttc ccaatcttcc gtaaaaccag gaaaccggat
     3901 ttctggtcgt gtatcattgc tcatgtttga acacctccag cgcaccttgt aaccacgctt
     3961 cattttcagg tgttgtttgc agggaagaga tagcttctaa aagttctttt tctagcactt
     4021 gctgttcttg tcgtatcttc ttgatgtcat ttccgacatg aaccatatca attggttctt
     4081 cttcttcgaa agtatcgaca tagcgtggga tatttaagtt atagtcattt tctttgattt
     4141 catcaaaggt tgccaaatga gcgtatttct cgacatcttt tctttcagcg taggtttcga
     4201 ggattttttg aatgttttct tccgataatt tattctgatt tttcccttta acaaattcac
     4261 gactagcatc aatgaataat acatctcgag tttgacgatt cttcttcaat acaatcaccg
     4321 tcgttggaat cgatgtacca aagaacaagt tcgcaggcat accgatcaca gcataaatac
     4381 tgccgtcttc taataatttt tgacgaatga ccccttctgc agcaccacgg aataaaaccc
     4441 cgtgtggcaa gacgatagcc atagtccctg tttccttcaa atgatagaaa ccatgaagaa
     4501 gaaacgcaaa atctgctttg gattttggcg ctaatttccc gtagcgatta aaccgagaat
     4561 catctaagaa ggtcgtatct gctgaccaat tagccgaata aggtggattc atcactactg
     4621 catcaaatgt gtaaggttca tctgttggcc agtccttatt caacgtatcc ccattgcgga
     4681 gattcatttc ttcggcatct accccatgta aaatcaagtt catttttgct agattgtatg
     4741 tcgttgtatt tagttcttgc ccatgatatt taacgttatc tggatgagtt aagtagttac
     4801 gtacatttaa catcaaggaa cctgaaccca tggttgggtc aaagacacta aagaatcgtc
     4861 gttctttttg atccaacgtc acgatttgtg ccatcatatc agaaaccata tgaggcgtgt
     4921 aaaattctcc ggctttcttc cctgcttctg aagcaaattg actgatcaga aattcatacg
     4981 catccccgat cacatcccca tcgtgaccta aaacttccac gtcattcaat ttcttgataa
     5041 cttcggtaat cgtcacattt cgttgttgct catctgttcc tagttttttt gattggagat
     5101 ccacatcatc aaataaccca ttaaattgat tgtaggtaga tgccaattgc acaaaggctt
     5161 tgtttagatc gttcaattgg aacgttgctt gttttgcttg atccgctaag acgttgaaaa
     5221 gatactctgg tgagatcgaa taccccaaaa tatcaacgat tgtggcaatc aggtcttctt
     5281 ttgattcttc atctgaaagt aattcacgat acaacattgt ttgtttgctt actgtgtcat
     5341 attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaaacgg tcggataaat
     5401 atttatagaa aatcagtccc aataaataat ttttatattc tgacgcatcc attttactac
     5461 ggagattatc tgccgcacta aataattttt gatttaattc tgcacccaaa aagatacact
     5521 cctatctctt atttaaagtt gctatatacc ctcttatttt agcatactcc cttttagaaa
     5581 aagcaaaaaa aaaagaaaaa atcttattct tgataacaag ctacgtgacg tatatgccta
     5641 acaaaaatag aacttttgaa tcaatcttaa atgaaggttt aaccacagaa ccggcatcca
     5701 ttatttttga tgaagttatc gatgacgttg aagatagtaa gtatcataaa aatttaaaag
     5761 aactttttta ttttgcactc aacaatggac caagtttgtt cactggtata tcagtacctg
     5821 atagtgataa tccattaatt atggcaaaaa tttacttaac aaaatggtgt aataaataca
     5881 tcagggatag gaataaccca gcattaaaaa aacctcttaa aacatttggc gaaaaagatg
     5941 ctgctttaac tactagagta gcagcaaatg ctaatattga tgatcaaaaa gtattgaatg
     6001 actacttgcg tggacatttt ttatatatga gcgctgagaa catgaacgga agtattttag
     6061 aggaatattt agccgaagtg ttagaacctg aaggttggat ttggtgcgcc ggctctgtat
     6121 atcgagctgt tgatttctgt tatttaggaa cctcaccaat tcttttacaa gtgaaaaata
     6181 aatacaatac agaaagcagt tcgagttcag ctataagagt aggaacaaca attagaaaat
     6241 ggaatcgttt aaataagtcg acaaaaattt ctggtttgga tagccctatc cccaattgga
     6301 aagcattaat agaaatgact gaagctagta aggaactggc agctaagtta actgaaaata
     6361 gttatttagc ttatattaat gaaaaatcca cacgagaatt atggacactc gatgactaac
     6421 aatatcctta ttagtatcta tccacgatac atggaaaaaa ttataaacaa acaaaaaaac
     6481 tttgaattta gaaatttcta tcttacctca gatccttata atatcttttg gatttacgaa
     6541 acaatcccca ccaaagcttt aaagtataaa ttgattgtaa aaaatccaat tactaaactt
     6601 aagaaaaatc aacactactt tttaggagac gatgagtttg aacaattagt ttaaaag
//
LOCUS       DACDIP010000097         6632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDIP010000097 DACDIP010000000
VERSION     DACDIP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.097"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            148..2823
                     /locus_tag="IV257_002562"
     CDS             148..2823
                     /locus_tag="IV257_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8815644.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3005..3244
                     /locus_tag="IV257_002563"
     CDS             3005..3244
                     /locus_tag="IV257_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815645.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3260..4633
                     /locus_tag="IV257_002564"
     CDS             3260..4633
                     /locus_tag="IV257_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8815646.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4637..6271
                     /locus_tag="IV257_002565"
     CDS             4637..6271
                     /locus_tag="IV257_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8815647.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6434..>6632
                     /locus_tag="IV257_002566"
     CDS             6434..>6632
                     /locus_tag="IV257_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815648.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2322 a         1071 c         1332 g         1907 t
ORIGIN      
        1 ttgtgatttc caaaatcaca acttttttct tttgtcaaga ttttgagtgg gatatatttt
       61 tgtagaactg aagatcatga aattatgaga ggttggaggt aatgtagtca tacaaataaa
      121 aattttttag aaagtgagtg gtaattaatg aaaatgaatc acacttcaat tcagaagcat
      181 ttacaactgt taaatacact ggggatttct tatgttaatt tgagaacttt ttctacatta
      241 aacaagaaaa ccgtttctca tattttttct actgcagatt ctcaagcaat tattaactat
      301 ttagaaaaac agaaattgtc tgaaggtaca gtaaacataa cttataatcc tattaaacaa
      361 gaggtgatta cacaaaaaaa gcattctgtg agagacaatg atattgaaaa aattcggttt
      421 gtatttattg atattgaccc taaaaggaaa gagggaagtg caacagatag cgaaaagaaa
      481 aaggcagaaa atgttatgga acaagtagaa cgttatttga aagaaaagag aatagaatca
      541 tttgtaaaag ttgatagtgg gaatggctac catatttttc ttcccataaa tgaacaacca
      601 aataatcacg agacgattct tactattcag aattttcttc aattacttca ccgaagattt
      661 ggagtagaag atgaagttga tattgatact tctgtttata atccttcaag attatgcaaa
      721 ttagttggta caccagcggt caaaggaaag tccacaaaag agcgtccgca tcgtttatct
      781 agtttccttt ctattcccga taacatagat agaagtaatt gttttaaaat tcttgaacag
      841 atagttgaag aaaattccat gatgtcaacc tctagagtaa aaagccatcc atcttttaga
      901 gaagtgttac cttttattca agcagactat aaacaatggt tagctcacta tcctgaatta
      961 acttatacca taaaagaggg cgatcataaa ggagttacct tacttatatt tgacgaatgt
     1021 ccattaagaa aacacacgaa taattctaat ggttcttgtt tgtctgttac ggctgataat
     1081 aagattcgat ttcattgtct gcatgcttcg gataaaaata aagatattca agactttgta
     1141 aaaaaatatc cgttgccaaa acctgtaaat ccttctgtag aatcattaga atccaatcaa
     1201 gaatattatt ttgaccagtt taaactgaat caagaaggag tatttttggt gaaagaaggt
     1261 caagaagaaa aagttgcttg tccgatgttt attaagcaaa tacgcaaaaa tatcgaacaa
     1321 aaagtcatta cacttgttct tgcctattat gtcgatcatt gttgggatga aatagaaata
     1381 gaaggtggat gtttacaaac taataatttt aaacaattag caaaacatgg cttaatcatt
     1441 aaagctagaa gagaagctga agtcgttgac tttttacaga ttcaacggga gaatgctcca
     1501 atcttttttg aacatgaatc tcttggctgg aaaaaagaaa atgaagcaat gatttttcag
     1561 ttacaagatt cttttggacg tgaaattcct tctgtattaa ctaaaaactc atgctaccaa
     1621 ttaacaacta atggtacaga agcggaattt gacgcattgg ttcataagta tgttttagga
     1681 acgaacttag aacttgcttg gtcgattggt attgcttcta ttgtgttagg ctatttaaac
     1741 gttacgaagg ctaatacttt tttgagttta ctaattaacc tacagggcaa gagcacaacg
     1801 ggtaaaacca ctacgttaca ctttattgcc tctttatttg gaaatccaac tacggttctt
     1861 agaaatatga atgctaccaa taaagccatt ataaagctag catcaaacaa tcagggaggt
     1921 gttccactaa tactagatga gttaggagca gcaactcaaa ataactttag ctcgttgatc
     1981 tatcaattag cttcaggaga agaaaggcta agattagatt ctaattgtca gcttatgcca
     2041 caagagactt tttctgtttt gacccttacc tcttctgaag agcgtttgga aaattatttc
     2101 aatgagaaat taactggttt gaaagttcga tatatcgaat tttctgatat caagtttaca
     2161 aaggatgcga atcacgcaga gaaagttaaa agtctttctt ctaatacttt tggttggggt
     2221 ataaagttat ttcttttgaa actctttgaa caagggatta caaaaattga agcaactttt
     2281 gtcgaagctc aaaaaaaact atgggctgtt ttacctgaac attctttgaa gcaacgcatt
     2341 ggtaataatc tagctttgat ttatagtgga gccctcctaa ttcaagatat actgggctat
     2401 cctttacgat tagagcatat acagacggtg cttattaatt cgtacgaaca gttaattgta
     2461 gatactagtt taggaagtga gcaaacggta tttgaccgag tgaaaacatt gttggtgatg
     2521 aatagccata aatttttaac aaacagtgac aacaaacata cgcccagtac gatttgggga
     2581 aaagtatcaa ttcaaaaaaa tggggatatt aaggtaaata tttttcctat cccgtttgag
     2641 gacttgttaa ggcgagaatt tcaagtaaag gatctaacct atctttttag gcaattgctt
     2701 gattcgggta atatgtgtgc agaaaaaggg agaagaacga aacgtattca catgaatcgt
     2761 caagttcttc caacatacga gattctatta ccttcttcac tcaaatcgta ttttcgattg
     2821 taatttagaa taaaccaaag tatgaaagtg aggcaccatg ttatggaaat atttcttatt
     2881 gatttagcaa aacaactgat tacttatctg tatgatagcg agcaaaccca aacggaagta
     2941 aatgaagctt gatctatgct tcatttactt cattcaatca aacatgaaag aaaggaaaat
     3001 tctaatgact aaaaataaaa tgacactaaa agcagaggtt cttctgtata ttcaagaaca
     3061 tttttcaaat caagcctttt ttacaaaacc aatttacttg gatttcgaaa taagaggggt
     3121 atctgctgga tctatcgggg gtactcttca agcgttgaag aacgaaggat atttagaaaa
     3181 tcactttgtg caacgttcgt tcaatagaag agtcgtgaaa aaatggtatt tagttcattc
     3241 atagagaggg gaatcaagca tgcgtgtggc ttgttatgat cgaacgtctg tttatgaaga
     3301 agcgaaaatt agtaatgacg ctcaatatga tgcagtgaaa gagtatgttt tagagaaata
     3361 tgaacaaatt ccccaacgat tttcagacaa aggagaatca ggggaaaaag cggagcgtcc
     3421 agcttttcga cagcttagag cttgggtttg tgcgtcagaa gaagaggggg tgatctatgt
     3481 ttggcgttat gatcgattgg cacgtcaagc caaaatagcc ttagaattta ttgagttgtg
     3541 ccaacaacat caggttcaag tgtattcgat ttctgaacct cttcctgctt ctttctcaca
     3601 cgcgtcaacg aataaactat ttgtgcaatt attgttcact tttgcagaac aacaacgaaa
     3661 cgtcattatt gagaatgttc gtgccggact ttcctataaa aagcaacaac ggcaatattt
     3721 gtcttctgcg gtaccatatg ggtatcgttt agtgaatggg aaaatccaac aagaagtaca
     3781 cgaagcaagg attgttcgct atatttttca actttatttg accaaaaaat atggctacaa
     3841 aaagctttgt caacgattga cgcaacaaaa gcttttcttc cgcgaacgtc cttttcagcc
     3901 ctatcatatt tatagtatcc taaaaaatcc attgtactat ggcgaaatca aaggaggatc
     3961 tttggggaag tatctaggaa cctttgaacc gattctctcg aaagcaacct ttttacaagc
     4021 ccaagagatt cgccaatcaa ggtgtacagc taaaaaagat acgtatctgt acttattacg
     4081 tcaaaaaatt cggtgtccct tctgtggtcg tcatctgagt tcgaagtatc aatggaatac
     4141 aaagaagacg aagacgcttc attattatca ttgtaccgac cgaagctgcc aaggaatttt
     4201 tcttcgggca gaaagggttg agtcagaagt attggaaaag atacaggctt tttttcaata
     4261 cgaaggcgtc tatcaagcct tgattgccca attaacgcaa caattagccg aaaagcgaaa
     4321 agatgaaaaa cgcaaagtaa tccaacaaaa gaaacaagaa gcacaatgga ttaaggcatt
     4381 tgaagagggc aattgttcac tggaagaact caagcaacaa ctgcaagctc ttcaaaagcc
     4441 gatgtctact tcttcgtttc tttcactcca acagtgtgaa gcaaaagtga aagaatggct
     4501 tcaactaaaa gacctctcta gtcgacaatt attatgtgac tatgtttgtc ggattgaggt
     4561 aacaaaggat aaaacagttt gtgggattta cttccgacaa ctgacacaaa atattttaga
     4621 aaggaacgaa tgatctatgg aaatgataca aaccaaacga gtcgcattgt atattcgcgt
     4681 gagtacacaa gaacaagctt tagaaggaca tagtattcaa gcacaagaac aaaatggacg
     4741 acagtttgcc gaacggatgg gctatgaagt ggtagaagtt tatgccgatg aaggaatcag
     4801 tgggaaatcc acaaaaaatc ggttcgccta tcaacgaatg atgaacgatg ctcggaacca
     4861 tcgttttgat ctactagtga tttggaaatt gacccgtctc ggacgcaata tgttagatgt
     4921 cttacaaaca gtcgaggaat tattgcgata tgacgtagga ttacattcga tctcggaaca
     4981 atttgacgtg accacttcga caggtaaatt gatgttacaa ctccttggga gtttcggtga
     5041 atttgaacgc aaccaaattg cggagaatgt tcaaatgtca atgcaaagct tagtgaaaga
     5101 taaaaagcgt tatgcaggag gccgacgttt aggctatgtc agtggtattg acacgaatgg
     5161 tcaaaaacaa ttggtcattg aaccagaaga ggcgaaaatt gttcgtctga tctatgcaaa
     5221 atatttaggc ggagcagggt atcgagcgat tgccaatttt ttgaatcgtc aaggatatca
     5281 aacagtcaaa aagaatgctt tttctaccac ggcagtcaaa gatatcttac gcaataaaat
     5341 ttatgcaggg tatttagaat atgcgcgcta catcaattgg gaaaccaaac gacggaaggg
     5401 gaagaatccg agaccgattt tagtgaaagg aacccatgaa ccgattattt cggaagaaga
     5461 ttatcaaaaa gtacaagaac gccttgcttt agaaggaaaa caacccaaat ggaaccatac
     5521 aggtgaaaat gtattgacag gcttgttacg ttgtccagaa tgtggcgcac cgatggcagc
     5581 gagtaatgtc acaaataccc taaaggatgg cacaaagaaa cggattcgat attattcttg
     5641 ttcagtcttt cggaataaag gagcaagtgt gtgtcatgcc aattcgattc gtgccgaaca
     5701 agcggaaaca tttgtcgccg accgattaaa ggagatcgtt cagctaccag aagttcttcc
     5761 ccgattggtg gcagcgttaa atgaagaaat agtgcgacaa agccaaccat tggaacaaga
     5821 actcgtggtg ttactggaac gaaaggaaga attgaagact aaaattgaaa aatgggaagc
     5881 tgctttggaa gatagtcctg aattgtttcc gatgttaaaa gaccgcttag acgaattgac
     5941 tgaaaaacga cgacagttgc acattcgaga aaatgaaatc ttagggatct ttcaacaagg
     6001 agagccgatt caagtcaaag atgtccaacg gatattgact agtttggatc gttttcttgc
     6061 ccagagtgag aagaagcaaa tcaaagcctt gtatcgtacc tttatagaga aaataacctt
     6121 tgatcctaac cataaggaga tgttggaaat tacgatgaaa ttcacgcatg ccgtggtaca
     6181 acaattgaat gagcaatatc aaatagccgt atccaaaaca aaggatacgg ctatttttgt
     6241 gttaaaaaca ccctttacgt taacgatcta gtctaggtta tatcaaacaa cgttagaggg
     6301 gcgctaacgc ttctctagcg atttgaaatc gttagagaag tagttaggga ggtccttatc
     6361 ataaataggg atatttatta atgaatattt gaaatataat tgtatataag aattaaaaag
     6421 agtggggata gatatgaaaa agatgggtaa gtgtatttta tgtgaaaaat acactgaact
     6481 tactaaagaa catgtaccac ctaaaaaatc agggaatact ggaggtaaaa agaccagaac
     6541 tggtaattta gatgatttcc ttaagagcga ttttacaaaa ggtgattttc cgaaggggat
     6601 taaaaggaaa cctcaaggaa atgtttatta ta
//
LOCUS       DACDIP010000098         6341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDIP010000098 DACDIP010000000
VERSION     DACDIP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.098"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            290..1135
                     /locus_tag="IV257_002567"
     CDS             290..1135
                     /locus_tag="IV257_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8815649.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IV257_002568"
     CDS             1416..2636
                     /locus_tag="IV257_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8815650.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IV257_002569"
     CDS             2633..5353
                     /locus_tag="IV257_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8815651.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IV257_002570"
     CDS             5337..6281
                     /locus_tag="IV257_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8815652.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          982 c         1223 g         1773 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt t
//
LOCUS       DACDIP010000099         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDIP010000099 DACDIP010000000
VERSION     DACDIP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.099"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IV257_002571"
     CDS             725..811
                     /locus_tag="IV257_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8815653.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IV257_002572"
     CDS             complement(932..1621)
                     /locus_tag="IV257_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815654.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IV257_002573"
     CDS             complement(1628..1906)
                     /locus_tag="IV257_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815655.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IV257_002574"
     CDS             complement(1866..2780)
                     /locus_tag="IV257_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8815656.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IV257_002575"
     CDS             complement(2762..3142)
                     /locus_tag="IV257_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8815657.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IV257_002576"
     CDS             complement(3592..3879)
                     /locus_tag="IV257_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8815658.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IV257_002577"
     CDS             complement(3899..4123)
                     /locus_tag="IV257_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8815659.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IV257_002578"
     CDS             complement(4763..5296)
                     /locus_tag="IV257_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8815660.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IV257_002579"
     CDS             complement(5317..6159)
                     /locus_tag="IV257_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8815661.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDIP010000100         6103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDIP010000100 DACDIP010000000
VERSION     DACDIP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.100"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..478
                     /locus_tag="IV257_002580"
     CDS             <1..478
                     /locus_tag="IV257_002580"
                     /inference="COORDINATES: protein motif:HMM:NF013070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8815662.1"
                     /translation="SNKEELTNRLMNHLRPAIYRIRYELPFEIDDLAEIVKENHEVSA
                     VLNFVKESIVPLEAFLRKTIPETELYLISVYFCSELLNSSIIETPVRKKAIVVCSNGL
                     IMANVMAKTLKEIFPDIQFVASSSVREMDKFVEDVDLIFSNQPLKSEIPLYIIQPI"
     gene            475..1170
                     /locus_tag="IV257_002581"
     CDS             475..1170
                     /locus_tag="IV257_002581"
                     /inference="COORDINATES: protein motif:HMM:NF012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8815663.1"
                     /translation="MNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRYSAVQNEES
                     LQTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTWQNAMELSC
                     QPLLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGCSLLISVDP
                     IIFPGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTEDEVRKKIKN
                     FLRRKQYVNNEFS"
     gene            1148..1798
                     /locus_tag="IV257_002582"
     CDS             1148..1798
                     /locus_tag="IV257_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8815664.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            1785..2237
                     /locus_tag="IV257_002583"
     CDS             1785..2237
                     /locus_tag="IV257_002583"
                     /inference="COORDINATES: protein motif:HMM:NF012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8815665.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            2254..2559
                     /locus_tag="IV257_002584"
     CDS             2254..2559
                     /locus_tag="IV257_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HAP8815666.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            2571..3848
                     /locus_tag="IV257_002585"
     CDS             2571..3848
                     /locus_tag="IV257_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IIABC"
                     /protein_id="HAP8815667.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLIVGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            3867..5015
                     /gene="dgoD"
                     /locus_tag="IV257_002586"
     CDS             3867..5015
                     /gene="dgoD"
                     /locus_tag="IV257_002586"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP8815668.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            5027..5929
                     /locus_tag="IV257_002587"
     CDS             5027..5929
                     /locus_tag="IV257_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HAP8815669.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEE
                     LLKVMSEPTFNKMIEMNPINILSK"
BASE COUNT         2000 a          919 c         1297 g         1887 t
ORIGIN      
        1 tagtaataaa gaagagttga ccaatcgatt gatgaatcat ttacgacctg caatctatcg
       61 aattcgctac gaattaccat ttgaaataga tgatttagct gaaattgtta aggaaaacca
      121 tgaagttagt gcggtactta attttgttaa agaatccatt gtacctttgg aagcattttt
      181 gcgtaagacg attcctgaaa ctgaattata tttgatttct gtttattttt gttcggagct
      241 attaaattct tctattatcg aaacacctgt tagaaaaaag gcaatcgtcg tatgtagcaa
      301 tggattgatt atggccaatg taatggcgaa aacattgaaa gaaatttttc cagatattca
      361 gtttgttgct agttcttcgg tcagagagat ggataagttt gtcgaagatg ttgatttgat
      421 ttttagcaat caaccattga aaagtgaaat tccattatat attatacaac cgatatgaac
      481 gaagaagaaa aaaaccgctt gcggaaccgt gtactcattg atttttctac gaataatatc
      541 agcaaagaga ctaggaacct tactgaaatt attaagcgtt actctgctgt tcaaaatgaa
      601 gaaagtctac aaactgatat tgagagattt ttaattaatt atgcaaattc actaatatat
      661 ctagatcgtc aaagtaaaca ggcacaaaag atatcttttg tggaattgct tgtttctgaa
      721 gggattattc ttgtatctac gaaacaactt acttggcaga acgcgatgga attgtcttgt
      781 cagcctttat taaaggagca ggtaattaat cagaactatc agactatttt aatgagtcaa
      841 atggcggatg aagaggctta ttattttctt aataatcgta ttgctattcc acattcatcg
      901 ccagaaaatg gggtaattaa gggcggttgc tcgttactta tatcagttga tccaattatt
      961 tttcctggaa ataggcctat ccattttatt tccccttttg caattggaaa gaaggaaaat
     1021 tatttacttg cgattaacca attactacaa ctttctaagg acgaggaata tcagcaagaa
     1081 atacttcttt gcacgacgga agatgaagtg agaaaaaaaa taaaaaattt tttaaggagg
     1141 aagcaatatg tcaataacga attttcctga attaaccatc attttaagag gatatagttt
     1201 tgaagaggcg atgtttgtca tggaagaatt ggctgcgatg aacacttcgg taggaattga
     1261 agtaacaacc aataatgatg actttgcacg tatcatcaca gaaggcaaca aaaaatttgg
     1321 gcaacaatta ttgattggtg cagggacagt tttaacattt tcacaagcag atcaagcaat
     1381 tgctggtggt gcgaaatttt tattaggacc aaaaaaattt tcaacaccaa ttttttcgtt
     1441 ggctaaagaa cgtggggtgg taactgtgcc atcagctttt acgccatcgg aaattcaagt
     1501 tatgtttgat gaaggtgccg atattgtaaa ggtttttcca gcagtcactg tagggccaac
     1561 ctactttaaa caactggccg gtccttttgg tatacggcct ttaatggcag ttggaggagt
     1621 gacgattgat aatattgcgg cttttaaaaa agcaggagtt gtgtactttg gggtaggatc
     1681 aagcatgttt caaaaagctg atatacaaaa ccgcaatggg caagctattc gaaaatcaat
     1741 tcaatcatat ttagataaac tagctgaagc aggaagtgaa gaagatgagt ctcattaaat
     1801 tagaagaaaa aaatattttg ttaaatcaaa gtttttcaaa ccaagaagaa tactttcaaa
     1861 agattacaaa gaaactgatg acggaaaaca tggtaaagga gggatatctc gagtcaatca
     1921 ctaagagaga aacaacttat ccaacggggc tatatattgg ggaacgctca atcgcaattc
     1981 cacatacaga ttatcagtta tcaaatgtta ccgctatttt agtatccgtt cttaaagata
     2041 gtttgcattt tcagcgaatg gatgatcccg aagaaatggt ttcagtgaat gttattattc
     2101 accttttatt cgatagtccc gataagcaac cgcaattatt gaaggagttg atgagtaaag
     2161 ttaccaatca ggatttctta caagcaatta tggaagcaaa gagtaaaaca gaagttttaa
     2221 ctgcatttaa tcaataaatt ggagggaaat acaatgagta aaaaaacaat tatcgtcgca
     2281 tgtgcatcat caatgattac atctacaatg gcagctaata aaattcggaa aatagcaaaa
     2341 acaaatggat taccagaacc tcgaattttg caactaaaat tttcagaagt tgcaggtgct
     2401 ttagaatcaa atcaagttga ctttatcgtt cctacaggga aattacaaga aaatgtaacc
     2461 aaaggaatta aagttatacc tggaacacca tttgttactg gagtgaatga agaaaaatct
     2521 gaatctttgg tgttagaagc attgaaagag cttgactagg agggtgaaaa atgaatattg
     2581 tcattgatgc gttaaactgg atctcaagtt tgggtccgat gattatgatg cctatcatta
     2641 tcttgatttt aggattattt tttagaatta agctaaatac attactaaaa tcagcgttga
     2701 ctgtggggat tggttttgcg ggtgtaaata ttattattaa ttggtttatt tcacaggtag
     2761 gtccatctgt tcagttaatg gtcaaaaatt ggggtattca aacaagtatt atggatgtcg
     2821 gctggccagc tcgtgcagca gccacttggt cttttcctct agcagcagtt atggtattta
     2881 ttattttggg tattaatgtg ttgatgctga tttttaaaaa gaccaataca gtaatggtcg
     2941 atttctggag ttataatcac tatatttttg tagctgcttt ggtttattat gcaacggata
     3001 aaaatattat attggcggta atcttcgggg cgattgatgc aattattagt tttaaactcg
     3061 ctgattggac aacgccgtta gtagaggatt actttgaact agaaggggtt aatttcccga
     3121 ctgcgaactc agttggttgg gcacccttgg cttggctact taacaaattg tgggaccgca
     3181 ttcctggatt aaataaggta gatgctcgtc ctgaaaaaat tcaagagcgg tttggttttg
     3241 ttggtgagcc tatgtttatg ggatttgtca tcggcttggt tattgggtta ttagcaaaag
     3301 aacctgttga taaggtattg atagtgggaa tgagtacttc tgcaacaatg gttctaacac
     3361 ctaaaatgat gcaaatttta atggaaggat tattaccctt tgctaattct gttaagaaat
     3421 tcttgaatgt aaaatttcca ggaaacaatt ttacaatggg tgtggatgct gcgttgacag
     3481 ttgctaattc aagtgccatt gctacgggta ttatcatggt tccgattacc ttagtgttgg
     3541 ctgctatttt accaggtaac aaactcttac ctatttcaga tattgcctac caagcaatgt
     3601 ggctatctgc atggccggtc gccttttcaa aaggaaatat tattcgcgga atactttcca
     3661 ccacgattat aacagcttgt gtgcttttaa ttgctactag tttagccggc atacatacac
     3721 aattagcttt ggctggggga ttccaattac cagcaggaat gacacaaata tctaccgaag
     3781 atgcaggtac acacattatt ggtttcattt taatgaagat tgctgagttt atttcaaata
     3841 ttttctaata ttcaggaggg catacagtga aaatttcaaa aattacagtc tataaagtaa
     3901 aaccaagatg gatttttgtc aaaatatcta cagatgaagg tattgaaggc tggggtgaaa
     3961 tgatttctgg aactaagact gaaacagttg tggcaggagc ttatgagata ggaaatcgat
     4021 tcttagttgg tcaaaaccca tttgcaattg aacgtctgtt tcaggaaatg catcgttcat
     4081 ttttccgagg tggtcctatt aatggaacga ttgtctctgg acttgaaatg gctttatggg
     4141 atattaaagg gaaagcctta aatgttccag tttatgaatt actaggtgga gcagcccgtg
     4201 ataaaatcaa agtatattct tggattggtg gagatcgtcc ttctgaagta gtagagcagg
     4261 cacaagatcg agtagatcgt gggtttaccg ccgtgaaaat gaacgcgaca gaagaattac
     4321 actacattga ttcttatgaa aaagtagctg aagtagtgga acgtgttgct tcaattagag
     4381 aacgttttgg agatacattg agcattggtg tcgattttca tggtcgtgtt cacaaaccaa
     4441 tggctaaggt tttagcaaaa gcactggaac cctatcatcc aatgttctta gaagaagtgg
     4501 ttttgccgga gaatgaagaa catttcagtg aagtagccaa tgcagttgct gtaccactag
     4561 caacgggtga acgtttgtat acaagatggc aatttaaatc aattcttcaa cagggagcga
     4621 ttgatatcat tcaaccagat gtagctttat gtggagggat tttagaaaca agaaaaattg
     4681 ctgcaatggc tgaagcttat gatatagcag ttgcccccca tgcaccatat ggaccgattg
     4741 cgttagcagc tacattacaa gttgattctt gtacacctaa tgtgtttatt caagaacaaa
     4801 gcttaggtat tcattacaac aaagggtttg atttgttaga ttttgtaaag aataaagaga
     4861 tattccaata caaagatggc ttcgtagatc ttccgacaaa accaggttta gggttagaaa
     4921 tggacgaaga tcgagtgaaa gaaattgcac aagaaggttt agtttggacc aatcctaagt
     4981 ggaaaaatta tgatggtaca atcgctgaat ggtaggagat gagtgcatga tcaatacggt
     5041 tacaggccca atcgaagctg agtctttggg tacaacattt attcatgaac atttatatgt
     5101 cattcctaat gagttaagaa agtatgaaga ttataccttg gatgatattt atcatagtat
     5161 tgcggaagtt atggctttta aacaagctgg tggaaaaact atcgttgatt tgacaccaat
     5221 taattatgga cgaaatccgt tagctttgaa aatgatatct gaagctaccg gagtgaatgt
     5281 agcatttgtt acgggattcc ataaggaaga gtttcagcca aaatggttga caacgctcaa
     5341 tgatgatgag atctttgagt tgctttattc cgaaataact cacggagtca cttcacaaaa
     5401 ccttttgcca ggagcaatga aagtaggtac gagtaaaaat gaggttacat tggctgaaca
     5461 acgaatacta gccatagccg gtcaagtaca aaaaaaaaca gggattccca ttattacgca
     5521 ttgtgatggg gggacgatgg gactggagca aattaattgt ttaacaaaaa acggtgcgaa
     5581 agcaagtaat atttgtttga gtcatgtaga tttaactaaa gattttcact atttagctca
     5641 aatctgtgaa acgggagcaa gtatttgtat ggatcatatt ggccgagagt tggcgaatca
     5701 tgatgcagac cgcgtccaac taattaaacg actggttgat gcgggttatg aaaatcagat
     5761 ttgtctttca ggtgatatgg gacgaaaaaa atatttgcca acttatggag gaacgcctgg
     5821 gcttacctat attttaacaa gtttgaaaga agaactatta aaagtaatga gtgaaccaac
     5881 ttttaataaa atgattgaga tgaatccaat taatatactg agtaagtgaa gtcaattgaa
     5941 tcattcagaa gaactaatga aattagttgc tttcgcaggg ttaattcttc tctctgggtt
     6001 tggaaaagag ggcttagctt cggattccct agttaaacta ggggttttat caggttattg
     6061 tactgattat aagtacctga attattcatg aaatactttt tcc
//
LOCUS       DACDIP010000101         5999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDIP010000101 DACDIP010000000
VERSION     DACDIP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.102"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            55..1602
                     /locus_tag="IV257_002588"
     CDS             55..1602
                     /locus_tag="IV257_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8815670.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1711..2658)
                     /locus_tag="IV257_002589"
     CDS             complement(1711..2658)
                     /locus_tag="IV257_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8815671.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2836..3639)
                     /locus_tag="IV257_002590"
     CDS             complement(2836..3639)
                     /locus_tag="IV257_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8815672.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            3785..4699
                     /locus_tag="IV257_002591"
     CDS             3785..4699
                     /locus_tag="IV257_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8815673.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            4747..5217
                     /locus_tag="IV257_002592"
     CDS             4747..5217
                     /locus_tag="IV257_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8815674.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            5302..5910
                     /locus_tag="IV257_002593"
     CDS             5302..5910
                     /locus_tag="IV257_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8815675.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
BASE COUNT         1978 a          979 c         1186 g         1856 t
ORIGIN      
        1 gtttcttgtg gtattctgta acaaaatcag ttcgattcaa ggtggtgaaa aattttggaa
       61 actacagata cgttgcttca gttgcttcaa gaagcccata aaaccaatca agcacaacaa
      121 caaaccattc agaatcttac aactgaaatc caactattga atgaaaaggt caattattta
      181 accaacaaat tatttggacg ctctaaagag actctctttg aggaaacgaa tgggcaattg
      241 aatcttttta gtgatgaaga aatttctgta agtgttccag aagcagctgc caccattatt
      301 ccagtaaaag gacatcaacg agttgtagga acgaaagcag acaaaatcaa acacttacct
      361 ataacagaga aagaacatct ccttcctttg gaagaacaat tttgtgaaca ttgcggttca
      421 cagatgaaag atatcggacg gacaaaagta cgagaagaaa ttcgtttcca tcaggctatg
      481 ttggattgtc tcacccatta tcaacatacc tattgttgta aaagctgcga aaaagaagga
      541 ctttcttctt ttaaaaaagc gatcgttcct aaacctttga tttctaatag cttaggttcc
      601 aatagtttag tggcggaaac gatccggatg aaatttggtc agaaagttcc tgcctatcgg
      661 caagagaact attggaaaca aacacatggg ttggatattt cgagagataa tatcaccaat
      721 tggcatatta aagcagtgca aaacgcgtta gaccctttag gagaacgatt aagagtctat
      781 cttaatcaag aagagattct acacggggat gaaacaagtt atcgtgtgat tgaaagtgcc
      841 aaaacagata cctattactg gcagttttgt acaggaaaag acagtcagca tcccatcgtt
      901 tattatcacc acgacgaaag tcgtgctgga gatgttccga aaacttttct caaggagttt
      961 acagggtatt tacactgtga cggctatagc gggtataatg ccgtggaaag tgtacgactt
     1021 gtctattgtt tcgcgcatgt tcggcggaaa tttttcgaag caattccaaa aggaaagaaa
     1081 aacacggata ttcccgcagc tcaagcagtc aaacaattgg ataaatggtt tgtcctagaa
     1141 aagaaatgga aagacttttc tcctgagaag cgattaagct gtcggcaaca agaactgcgt
     1201 ccattattta ttgcttttta tgagtggatg gcaacgatag atcctgttgc aaaatccaag
     1261 ttggatgcag ccgttcaata tgcgtgtaaa ctacgaagtg gttttgaacc tatttttgaa
     1321 gatggtcgtt tagaactcac aaataatcgt gcggagcgaa atatcaaaga gctggtgata
     1381 ggacggaaaa attggttgca ttccacaagt cttgaaggag cacgaacttc aggaatcatt
     1441 ctgagtgtat ataaaacagc agaacttaac ggattgaatc ctgtaaagta tctagagttt
     1501 ctatttgata agataccaaa tcttcccgta ctttcagcag aaactttaga tcaactgttg
     1561 ccttggaaca aggatgtaca acaacatttt tctcgtaact aaaaacgcca aacattcccg
     1621 attctattct ataggtcggg ttatttgacg tacaggtact gttctatggg gcgcttacct
     1681 tttttctttt gaaaatattg ttttcagcta ttaatatcct gcagacaatt ttacttgatt
     1741 ggtttgaagc tcatgggtgg caaagtagct tttcagattg gttaaaaaaa tagccattaa
     1801 tttcttttga aaatcaggtg tcaaacctgc aatgtgggaa gtaatcagta cattgtccat
     1861 tttccaaaga ggatgatctt gtggaagagg ctcttcctca aaaacgtcta atgctgcaaa
     1921 ggaaagttgc tggttattta aagcctgcac cagatcttgt gtcttaacag aagcaccacg
     1981 gccaacattg atgaatacag attttggatc aaagtgttca aacatatgct gatcaaagag
     2041 cccagttgtt tcctccgtta gaggtaagat attgactacg aaatctactt caggaagaac
     2101 ttcctttagt tgatttaaag aaaaagtctt ttccattcct tctgctggat gaccggatgt
     2161 attgattcca tagcagttca tgtctaatgc tttgcatttt tttgcaatca tttttccaat
     2221 atggccagtt ccaatgatta gtattttttt cccttggata gattggtaat ggaccgatga
     2281 acctaaccat ttcatgtcac tctgtgcctt ttgtgcttga aaaagaccac gagaataccc
     2341 aaggatgatt cctaagacat gctccgtaat cgattcactg tggatgccac taccatttga
     2401 aagtaaaata tgtttctttg aaaaagtctc aaggggtagc tgatcaacac cagctgagat
     2461 agattggacc catttcaagg tagaagaggc taatagtttt tcttggtagt ctgagttcca
     2521 gcccaaactg atctctacat tttgtagatc ctctggttgt aattctgttt taaagatata
     2581 accaggcgct atagtatgga tttcttcgat aaattcatca cgaaatggac gatctgcata
     2641 aatgattctt tctgacatag ttaatttccc gcctttcttt actctaagtg tagcgcatac
     2701 aagaagaact ttgaagcgcc ttgcttgttt tttgagaaag aaatattttt caaataaaaa
     2761 aggggctgtg acaaaaaaat gtcacaacct ctaaaagggt ctattgaagt agaaataacg
     2821 agttgtcttt atttcttaat tgatcgatat ttttgcccat cctttgacag cttgagcaaa
     2881 aataaagatt cctgcaaaag caatgaaggc gctagtgtca aaagaaggaa gaaagaccat
     2941 caagattgtt gcaattatag taggcaatga aattgaatag atcaatgttt ttaaacaatc
     3001 aagaaaagta gcttttgtta gtcgaaagcg tgcatagaga aaagcagcaa tgttggtaaa
     3061 tagcaaagtg atcagcagat tcagaaaact aggataaata gaaaatataa atgtcaggac
     3121 ttttatccaa agtggtatag atgcttcact taaaaaagaa cggagctgct tacctgttaa
     3181 gttttttaaa gattcattgg tgtagggaag ttctaattgg ttattgttca acaaagcaga
     3241 agtcgtgcct gtattaggaa gagctatgat aagtttgttt tttaacatcc cgacactaac
     3301 aaaattcccc atcaaatcag tagagatgtc ctgttctgtt cgttttccct ctggatcaaa
     3361 tgtgaagata atggagtttg tttggtaaat gaatccttct ttttcttcgg tttgaagctg
     3421 cccgttttgg attgtaaaat caggaatgcg agtagcaatc ttttgcccat ccaattgaat
     3481 gttgtttaag acttgaaaaa cttgaaaaga gattggcaaa gccataatta tactcaaaac
     3541 taaaagatag acaatggctt tccaaaatgg gacatttctc gcctccttta ggtctttgaa
     3601 tcgtataaag gaactgataa ttaattgttt tgttgtcata agtactcctc atctagtagt
     3661 gtcatcactt gcaattctag cgaagtatca agtgaaatac aagtgcttta aggaaacagc
     3721 tgaatgattg gtgaaatatc aaaaaaatgt tattcttgtc taaagactat ccaatataaa
     3781 taaaatggag gaaggaatag tgcctacaca gccacagaga tcatcaaaaa aactatttgg
     3841 agtagtggtt ttgaatgcgg tgatcacttt atcagaattt attggaggga ttatgtcagg
     3901 gagtttgtct cttgtttcag atgctttcca taaccttagt gatacattgg caattatttt
     3961 ttcttacggg gcacaaaaaa tggcccgaaa agaagcaaat aaaaaacgta cgtatggcta
     4021 ccagcgttta gaaatattga gtgctttcat caactcattc atattgatca tattatcctt
     4081 gtttttaacc gttgaagcat tcaagcggtt cacttcacca gaaaagataa atagtcattt
     4141 gatgctaaca gtagcagtta ttggattgct cgcgaatttg ttctcagtat tattattacg
     4201 acaagaagca gatgaaagtt tgaatataaa atctagttat ttgcatttgt taagtgatac
     4261 actgtcttcg gtaagtgtga ttatcggagc aattttgatc cgttttttcg atatctactg
     4321 gatcgatcca gttattacac tgatcatttc gatatatata ttgatagaag cgataatggt
     4381 gatcaaaaaa gctgccgcta ttttgataca aagtgcacct gctatcgact atgaaaaaat
     4441 ggaacaagaa attgaagcaa tcgatggggt aaaggatgtt catcatgtcc atatttggca
     4501 gtatagcgaa aagataatta ttttcgacgg acatattgat tttgaagatc agctactaag
     4561 cgagattgaa aagatatatc ctcgtataat ttctttatta aaattaaaat atggtattac
     4621 acatgtgacg atccaagcag agacaaaggc aaaggatcaa aaaaaattaa tttttttaaa
     4681 taaagatgaa agttattgaa atatagagta tgataaataa gaatttttaa taaggtaggt
     4741 tggctcatga agcagtatcg acagttaaaa ggatatttag aagcaaaagg atattgggag
     4801 atactagtct atctcttttt tggtggtttg gctacgattg taaattttgt tagttttgct
     4861 ctcgcacgcc aatattttga tttaagtatg gccttatcca attctatttc gtggttttgt
     4921 tctgtattgt ttgcttttgt aacgaacaaa ctttgggtat tccattcaaa atcaccgaat
     4981 tttacacatg cacttattga atgcggtaag ttcttcttct accgtatcct ttcttacgga
     5041 ttggacatgg gggcaatggt attgttgatc aatgttatga acagcaatga gtatgtggcg
     5101 aaaatcatca ctcaaattat tgttatcctt gcgaactata ttttcagcaa gttgtttatt
     5161 tttaaagaaa ctgaggtttt tgaagaagaa gtaggtcaaa gtgaaaaaga cgactaggca
     5221 taacaattga agaatttcct tctctttgtt actctttgta atgtagacat tattaagaaa
     5281 acattgagga ggaatttttt tatgacttac acattaccag atctacctta tgcatatgac
     5341 gcattggaac cttatattga cgaagaaacg atgcatctgc atcatgataa gcatcacaat
     5401 acttatgtga cgaatttaaa ttcagcaatt gagaaatacc cagaattagg cgaaaaaaca
     5461 atagaagaat tattatctga tatggacgct attccaacag atatcaagac agctgtacgt
     5521 aacaatggtg gcggacatgc taaccattca tttttctggg aaatcatggc accaaatgct
     5581 ggtggcgaac ctacaggaga aataaaagaa gcgattaatg aagcttttgg tgatttttct
     5641 tcttttaaag aagaattcaa aaaagcagcc gctggacgat ttggttctgg atgggcttgg
     5701 cttgtaatgg aaaatggaaa attagctatt acctctactg caaatcaaga ttctccattg
     5761 atggaaggaa agacaccaat tctaggtttg gacgtttggg aacatgctta ttatttgaaa
     5821 tataaaaatg tccgaccaga ttatattgca gcattctgga atgttattaa ttgggatgaa
     5881 gtaaacaaac gtttcgaagc agcaaaataa ccatttcctc cgtcatatca gtcttaaaaa
     5941 taatttggaa aataaaagag cgaaagcttg ctagcgtttt ctaaattggg tataatgtt
//
LOCUS       DACDIP010000102         5913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDIP010000102 DACDIP010000000
VERSION     DACDIP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.103"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(124..609)
                     /locus_tag="IV257_002594"
     CDS             complement(124..609)
                     /locus_tag="IV257_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815676.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(748..963)
                     /locus_tag="IV257_002595"
     CDS             complement(748..963)
                     /locus_tag="IV257_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8815677.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1187..1987
                     /locus_tag="IV257_002596"
     CDS             1187..1987
                     /locus_tag="IV257_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8815678.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2006..3874
                     /locus_tag="IV257_002597"
     CDS             2006..3874
                     /locus_tag="IV257_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8815679.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3867..4358
                     /locus_tag="IV257_002598"
     CDS             3867..4358
                     /locus_tag="IV257_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8815680.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4346..4900
                     /gene="srlA"
                     /locus_tag="IV257_002599"
     CDS             4346..4900
                     /gene="srlA"
                     /locus_tag="IV257_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8815681.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4918..5913
                     /locus_tag="IV257_002600"
     CDS             4918..5913
                     /locus_tag="IV257_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8815682.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2066 a          945 c         1193 g         1709 t
ORIGIN      
        1 gaaatagctg taacgaatta ttaggttaat gcaaatgctt atttatactc cgtataactt
       61 tctcagaaaa aatgatacca ataatgactt gctagtactg taaatccgta taatatcatc
      121 taatcaaaat ggtgttccat ttgcatctgt catcgtaatc gatccttcgg gtgacactcg
      181 ataaaaacca acgcttccag atcctccttg ggaacggatt tgtttgctgg aagctgtgaa
      241 caaataatct cgatcaattt tttgataaaa gctaataaca atatcgtcac tttgtaattc
      301 ggaacggtgg gcaataagaa atagaagtgc tttgtcagag ttttctaatg gaagagaagg
      361 tacagtgctt tcttgcattt cagatgttga agaaatgcta aacgactgaa tacttgattg
      421 gctatgttgc gaatctacat taccagtaga agtattagta atggaagtag tagaagtact
      481 tttagttgaa ccaacctcag ttgttgaaat ggctgctgaa gattctttaa cagaattctt
      541 tgttttatat ccacaggcag aaaaaagtat tacagttccc aacaagcctg cgtaaatccc
      601 tttatgcatg atagctcctc ctaaaataga ttatctttat tcagtatatc acttaaacaa
      661 agaaaagcag atggtttaaa atgaaaaaac cttcccaata aagggaaggt tcatgaagat
      721 aataactttt ttaataatta tagctttcta atatctttca tcaacttttc tagtaatcat
      781 tgggaaaaat ttcaagcagt agatcgcaca aatacctacg acgatataaa tgatcttcgc
      841 aaaaatagtt gttgaaccac cagcaatcat agctactaaa tcaagttcga acaagcctac
      901 caacagccag ttcaaccctc ccacaatcaa taaagttaat gcaatagcat ctaaagtttt
      961 cattagaatt ctcctttctt aaatgataaa acaattatat atcaaaaaga gaaacattgc
     1021 ttatataacg ctcaggaatt ttagcaatct atgcaaaaag atgaaaattt tttttgctct
     1081 tccattagta cacatttttt agctgaagct gtaaatttca tgtcttaatt ttttttagtg
     1141 agcttttata ctggatttgt aagaaaaaag aaggagagga tcacatatgt cggattggtt
     1201 ggatattgca ggaaaagtag taattgtaac tggaggttcc tcaggaattg gaagaagtat
     1261 cgtcgaaaat ttgttgaagc agaatgcaca agtagcgaat tttgatgtaa ctgaatgccg
     1321 tatacagcat gaaaacctac tatctttaaa agtcgatgtg tcttctaaaa cggacataga
     1381 agaagggata tataaagtaa tgaaacattt caagacgatc gatggtctag tcaataatgc
     1441 aggaataaat attccttctc ttctaataga tagaaatcat ccaaaaagca agtatgaatt
     1501 atcagaacaa gtttttgata agatgatcgc tgtcaatcaa aaaagtgtat atttgatgag
     1561 ccaagcagtt ggaagaatct tggtgcaaaa gggatcagga gtgatcgtga atctttcttc
     1621 tgaatcagga ttggaaggat cagaaggaca gagctgttat gctgctacaa aagcagcgat
     1681 gaacagtttt actcgttctt gggcaaaaga gttaggaaag aggaatgttc gtgtggtagg
     1741 tgttgcacca gggatactgg aagaaaccgg tttgagaaca caagaatatg aagaagctct
     1801 ttcttatacg agaggtataa gcgtcgaaca gctgagaaac ggttacagtc gaacaagtac
     1861 gatccctcta ggaaggagtg gtaagctgca agaagtggcg gatctagtct gctattatct
     1921 ttcagaaaga tcaagttata tcactggagt cacgactaat atttcaggag gtaaaacaag
     1981 aggataaagg atagggggga cgcacgtgac gctagtaaac agatggtacc aattattgaa
     2041 attactagtc actcacaaga aagtttcaat ccaggaagtg caaaaagaaa tcaaaaccag
     2101 taatcaaacc ataaaaaaag atattgggga gttgaatgaa gaaattgccg gtattgcaaa
     2161 aatcatccaa aaaaacaaac attttgaact tgaaatttat gattttgatt tttttgatga
     2221 gatcatgcaa ggaaaattga aaacagccag tgatttcaat tcggcatcca aaagaatcgc
     2281 ttatttacta aataggctga tcgaaacaga ggatttctta cgtatggatg atttgtctga
     2341 agaagtcggt gtcagcagag gaacagtggt caaggatcta aagacattaa aagagatgat
     2401 caatgatttt gatgtaaaga ttacaggcac accaaataaa ggactaagaa taataggaga
     2461 tgagctagaa ttacgactac ttttatttta ttttgtttct ccctactttt cagagacgaa
     2521 aacagaacag tttctagaag aaaaacaagc aatcaaacat tttcagaaga agacgaatgc
     2581 tagtaaacaa gaaatcaccc ttttaacgaa agttatcggt atttctttga atagaatctc
     2641 tagtaactat ctgttgaaga agcccatcaa tcgttacagt gggtgttttg aatatatcca
     2701 agaattcaat gaactgatca atcagttgga agaaatctat gaactaactt tgagccaata
     2761 tgaaaaagat tttatcagtt ttcctttgaa catcatcaac acaagtataa cgatgataag
     2821 gaaagctaac tctgatttgc gcttatattt cgaccccatg atgatgcgta ttcgacgatt
     2881 agatctcatt gatctggatg agtccttctt atatcaagag atgaaacagc acctctcttt
     2941 attgataaat cgggtaattt ttcgttatcg attgactgat ttattttatg gtgagataga
     3001 aaaaaagtat cctttttctt accaaatcgc tagcgaatgc atacaagcat tagaagctat
     3061 cttgcaacat aagatctcta aagtagagat cagctaccta acgttgtatt tggagttagc
     3121 atttcgatat caaaaagagc atttttcctc taaaaaagta gccattgttc atacgacagg
     3181 aaaaggaaca gcattaatga tccaaagaca aatcaaaagg atattgggga gggatataca
     3241 aataacacat gtttcagaag aacaatatga aaaagtagat ctaaacaaat atttcgctgt
     3301 ttttacaacg attccgttaa aaaatgtaaa tgaagagaca ccggtgatcc atttgactaa
     3361 tctttttgat gactcttggt taagggcaca atggcaaaaa gcaaaaaaaa agcgattagc
     3421 cgggtttcat tctactgttt ttttctatca aatatggaaa gaaaaaaaga cgtatcaaga
     3481 atatttaaac aaattagtcg ctcctttgat cagaggctat tatgtggatg aagaatttga
     3541 aaaaaggatc ttgaaaagag aatatcagca aagcacattt gttaacaaag aatttgcttt
     3601 tcctcacaca accaaccagc tatctcaaca cattgtttta tcagtagggc taataactga
     3661 tcaaggttta gcagaaacag gaggaaatgg agttattttt ttatttgcca tacctgaaga
     3721 aatgacggct gaagttgaga atgaattatt tgagctatac gatatcctat ttgaactcat
     3781 aaaaatggga atagatgagt ttattgaatt agcaccgaca caagaatcat ttttaaaact
     3841 aatcaaaagt aaaggagaga gacttcatga atgatatgta tctattcata gttttcgcag
     3901 cttcagctta tgtgctgcaa gcagttatca gttggaagca actaaagcat ttcaatcaaa
     3961 catatagcga actgcgaaga aaaggaaaag tagcgattgg taaacgacct gggaaaatcc
     4021 gttcaggcac gattgttatg ctagctatag acgatcatga tcaagtttta gaagtcagaa
     4081 aaatgcaagg ggttacatct ttggcaaaat ttcattcatt acctgtctat gcgggtgaag
     4141 atatccatta catcgatcgt taccatccct cagttcgagc ggaagatcgt ttgacacaag
     4201 cagctttaga aaatgccaga gaagtatact tacgaataga ggcaggaaat gaaaaaagtg
     4261 aacaaccttc attgcctttt attgctatgc tcaataattt tcggatatgg aaaagtacga
     4321 tcaaagaaaa aaggagtgac aaacgatgga tatcataaca aaatttgctg agggatttat
     4381 gaggctgttc caaacaggtg cagaaacatt cataagctgg atgacaggaa tcgtaccagt
     4441 tgtcttgatg ctgatggttg caatgaatac gctgattgct tttttaggtg aagaccgagt
     4501 aacgaaagta gccaaattat cagcgaaaaa tccgcttact cgttaccttg tgttaccgtt
     4561 cttatctgct tttatgttgg gaaatcctat gtcatttaca atggctcgtt tccttccaga
     4621 atactacaaa ccaagctatt atgctgctca agctcaattt tgtcatacga gcaatggtgt
     4681 attcccccat attaatcctg gagaattgtt tgtatggatg gggattgctc aagggatcga
     4741 aaccttaggg ctaaattcga tggagctggc tatccgatac ttgcttgtgg gactgctgat
     4801 gaactttatt ggtggttgga taactgattt tactactgga tttgtatgca gacagcaagg
     4861 aatcgttctt agcaaaaaag tagagctttc agttgattag agaaaggaga gttttaaatg
     4921 tatggtgata ttaagatcgt aagaggaaac ggcgggtatg gaggtccttt gattattagg
     4981 ccgacaaaag ataaacacaa gttcatctat gtcacaggtg gaggtgagag gccaatgatc
     5041 gtagataaaa tagaagaact gacaggtatg gagtctatca atgggttcaa aacatcaata
     5101 cctgatagtg aaatcgcgct tgcaatcgtt gactgtggag ggactcttag atgtggaatt
     5161 tatcctaaaa aagggatacc aaccatcaat atcgtttcca caggaaaaag tggcccatta
     5221 gcacaatata tcactgaaga tatttatgtt tcagcagtag ggttagaaca aatacagcca
     5281 ctaatggaaa ctgatgataa caaagaaaca gtaaatactg catctccaga taaaaagtac
     5341 aaatatgata cttctaaaaa gataaccgag caaaaagcag agcagcaaaa ctggatagca
     5401 aaaattggaa tgggcgcggg aaaagtagtt gctacattca atcaatcagc aagagaagca
     5461 gtagatacga tgatccggac agtgatccct tttatggctt tcgtttccct tttgattggt
     5521 gtgatacaag gttctgggat cggtaatgta tttgctaaca ttatggcacc tttagcagga
     5581 aatgcagttg gattaacaat cattggcttt atttgttcac tgccattttt atctcctttg
     5641 cttggtccag gggcagttat tagccaagtg attggaacat tgatcggtgt agagattggg
     5701 aaaggaaata ttcctcccca attagcatta ccggcactat ttgctatcaa tactcaaaat
     5761 gcatgtgatt ttattccagt cggtctaggt ttggaagaag caaaagctga gacagtagaa
     5821 gtaggtgtac catcagttct ttattcaaga tttcttaacg gcatcccacg agttatcatt
     5881 gcttgggtag caagttttgg attatatagc tga
//
LOCUS       DACDIP010000103         5751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDIP010000103 DACDIP010000000
VERSION     DACDIP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.104"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..434
                     /locus_tag="IV257_002601"
     CDS             <1..434
                     /locus_tag="IV257_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8815683.1"
                     /translation="KDYWGESKGFIYPDSNMGGNWKATNPKKEQEAMKLKNTKSNNLL
                     YATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSGGQNISSVSYETALL
                     EYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            454..1014
                     /locus_tag="IV257_002602"
     CDS             454..1014
                     /locus_tag="IV257_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP8815684.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGILSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADE
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            1838..2605
                     /locus_tag="IV257_002603"
     CDS             1838..2605
                     /locus_tag="IV257_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8815685.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIREKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            2631..3173
                     /locus_tag="IV257_002604"
     CDS             2631..3173
                     /locus_tag="IV257_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815686.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFATYESGMSAYDETGFDGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGYSLDDKTRLRLFDIEGKQIGLNKFCKNGKEFATKDF
                     IKGYRLLLENYGINLEESVK"
     gene            3174..3740
                     /locus_tag="IV257_002605"
     CDS             3174..3740
                     /locus_tag="IV257_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815687.1"
                     /translation="MFQKRKDKFPNLEVKSQKVIDGEMIELFQKNYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            3755..4003
                     /locus_tag="IV257_002606"
     CDS             3755..4003
                     /locus_tag="IV257_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815688.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTENLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4023..4262
                     /locus_tag="IV257_002607"
     CDS             4023..4262
                     /locus_tag="IV257_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815689.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            4633..4914
                     /locus_tag="IV257_002608"
     CDS             4633..4914
                     /locus_tag="IV257_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8815690.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            4904..5245
                     /locus_tag="IV257_002609"
     CDS             4904..5245
                     /locus_tag="IV257_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8815691.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIGEWEKAGLKKQSWIDVGSLKSIPKSRENISFKKVGMLSASDTKKL
                     NKFIERLQRKV"
BASE COUNT         2170 a          767 c         1161 g         1653 t
ORIGIN      
        1 aaaaagatta ttggggtgag tcaaaagggt ttatttatcc agattcaaac atgggaggga
       61 actggaaagc tacaaatccc aaaaaagagc aagaagccat gaaattaaag aatacaaaaa
      121 gtaataattt attatatgct acgtgtaaac attttcgaca tgtacgagat acagaattta
      181 cgagttatca tctttcagga atagtaattg acagtttcgt atatgaggct atgggaaatt
      241 ggaaatttgt tgaaaataac agtgggggtc aaaacattag ttctgtatct tatgagacag
      301 ctttattaga atactataac tcacataaag ttatgggcgg tttaaattta tactctcctg
      361 gaagcaatca atttgttaat tctgatagta gtatcatatg tttagaaaaa gtattaaaaa
      421 aaatagcttt ataaagtttt atggaggaac aacatgggag acgaaaaaaa gcgcttggaa
      481 gttcaattac gtgaagcata tggacgagta acgtatactt acaccactca tttaaagcaa
      541 gccgataata ttatttctga caataaaatt ttaaggtgtc ttcaaattgg tttgtcagcc
      601 cttactactg gagggatact tagtaccatt attactgata atagtgagtt aacctttgtt
      661 gctggattat gttcaacaat ttctcttgga ttaaacttat actttaagaa ctttgatctt
      721 gctgaagaag ctaaaaatca tcaaattgtt gccgatgaat tatggttgat cagagaagaa
      781 tacgtaagct tactaacaga tttaaatcgt ttaagtgttg aagatattgt gaaaaaaaga
      841 gatcaattaa tgaacgaaac tcatgaagca tataaaaatg cccctaaaac aaatagtaaa
      901 agctatgaat cagctcagaa agcattaaaa tctgaagaag agcaattttt tacagaagag
      961 gaattggata agatgttacc agagcatctt agaaaaaaga gttctaaata ttgaactctt
     1021 tttttatgct taaaattctc ttgtaatttt tctattcaat caataaataa tctatattta
     1081 agaagaaata tgatgataga aacatctcct aaagccaccg caagccgttc taagcatagc
     1141 ttagcccgac attgctagat atgctcatct caccatgaac gtttcctaaa gccaccgcaa
     1201 gccgtcctag tcattgccta gtccgacatc gctagattag tgcatctcgc cataaaagtt
     1261 ttctaaagcc accgcaaacc gtcctagcca tcgcctagtc cgattttgct tgatcggttc
     1321 catttctgtt tatttagtgg gtcaacaccg taatcttaaa tagccatcgc ctatttaaga
     1381 ttactaggtc tgctttgaag gtaaaaagtc tttcattctt caagactttt aggctttcaa
     1441 gctacgcctg aaagcattga aattcatcgc tctttatttg gttttgccat ttataattcc
     1501 ttacgcccat tatctttttc gcctgtcttg ccttgtcaga agtgtttaga gcttatctca
     1561 tctttggatt aatacaagtg gcgtaaattt ttttctaaac gcaagcgtta cgaaaataat
     1621 ttacttaaac acttgtatta atccggcaga taagaacgca ttctaatcac tcccgacaag
     1681 gcaagacagg cgaaaaagat aaataaatga acgaaaggaa gaaacataaa atgcaaaacc
     1741 aaatagctat gaagaaaaaa acacctccct ctaaaacaat ccaaacaaaa aagaattttg
     1801 aaaaaccaaa aatcaaaaac agaaagaagc gttcaacatg agagatagaa ttttagaagt
     1861 taatcaagaa atggtaaaca agttaacaaa tatcagtggg aactatgaaa agtacgatgg
     1921 tactttactt gataactata tttttcacga tacacaaaat atcagaattg gcagaagtaa
     1981 accgagagcg ttcattctca tcagagaaaa atatttgaat gaatggaata gcacctatga
     2041 aatggttatg actgataact ttgaaaaggt attggatttc agaaatgctt tgttgggttc
     2101 tgatatggga aatagcagtc ttgaatctat tgaagttgaa aacaagaccg agaatgaagg
     2161 tgaaggattg attaagaatg tcttgaaaat cgaacaaaaa tatatcagtg aagatagaag
     2221 tttttataga gtcacaagct cggttggtct agcaaacttt gctactaact taattggaaa
     2281 caatgctagt gaatctttgc tagtgatgtg tctgaataca aagaatgagg tcattgctta
     2341 tagcgaagtt ttcaaaggtg gaattaatca atcagtagcg ataccaagag agatttttca
     2401 actagctttg ctaaacaatt cagcaaaaat cgcagtatca cataatcatc cgagcggata
     2461 tgaatttcca agtgaaaatg acaaaagttt tactacgaat ttgaaaaacg caggaaaaac
     2521 aattggtatt gaattgttag accatatcat cgtaacaagt aaaaacacaa gctattactc
     2581 atttcaagaa aatggttctt tataaaaatt agagaaaaga ggaaaacaag atgggcgaaa
     2641 gaacacaagc gttaattaca gtcaaatatg gtaacagcaa aacgaaagca agcgtgtcga
     2701 ttcattatca gtgggggtat ggtcgatata tgctgatgga cgtattgaat caaggaattt
     2761 atttaatcaa taaagcatac gacttacgag ataaatcaag agaagaatta gcaaaaggaa
     2821 tctttgatag aagtacaggg gttatcaatt ttgccaccta tgaatcaggt atgagtgctt
     2881 atgatgaaac aggatttgat gggcgctatg aaccttatca agaaacgatt gaagatgtga
     2941 tgaagttttc tgataacaat gatggctatg ctcatttaga gatggtgttc gatgaaaatg
     3001 gatatagtct agatgataaa accagattgc gattgtttga tattgagggt aaacagatag
     3061 gactaaacaa gttttgtaag aatggaaaag agtttgctac aaaagatttt attaagggat
     3121 acagattgtt gctagaaaat tatggaataa acctagaaga gagcgtgaaa taaatgtttc
     3181 aaaaaagaaa agacaaattc cctaatttag aagtgaaaag tcaaaaagtg attgatggtg
     3241 aaatgattga actattccaa aaaaattatg gaacgataga aactaccgca agttgcaaat
     3301 actactttca aggaaagaga aacacgttta aaggctttgc cgattctgat gaagaattgg
     3361 caaagaaaat cattgattgg cttgtcagca gtgaacacgt agcaagagaa gataccgtta
     3421 gaacaacgat taatggggaa gtcctaacag gtactagatg tgtttgttgt aatcatgaat
     3481 ttgtagaaga tgaaggttgc taccgaatca atgaatatgg tgattttatt tgccagagtt
     3541 gccacgagat agaatttcca accgaaagaa gttggtttga atacatgcta caagattatg
     3601 agatggaaaa agaaaatggt gaagtagttt tttgtaccaa agaggaattg agtaagagaa
     3661 gcgatgatga aattgaagat tggtgtgtgt ggttagtagg ggcagatgat tgcgttgcat
     3721 attacacaac agcagaatag gagtgtgaat cagcatggaa ggaatggatt ttctagatca
     3781 tgaagatttg gttgattttg gttatacatg gaaaggaatg gtaggtatta gcagaagtct
     3841 tgctaatgcc ttttacgaaa gaaattatgc agtatatgtt ctttatgatg acgatacaga
     3901 aaaccttgtt gacgaggaat ataaattgga tttagagaat gttttatatg gaatagaaaa
     3961 ggaagattta gcaaaatata ttttttcatg gttgggtcaa taagtgaaag gaagatagaa
     4021 acatgacaaa atttaaaagt aagggtatgc aatggtgttc ttattattat gttggaaatg
     4081 atgaagattt attgagaaaa acccaacgaa aaggttatcc agataagata ttgaaacaaa
     4141 taggttatta catgccagaa gagaggatag aaaaagaatt ttgtgaggtt ctttttgata
     4201 gtgaacagct attagttttg ttaacccaaa ataaattgat ttgccaattt gaaaataatt
     4261 aagatgaggt ggtgaaccat gaatgaagat aagatggtat taagtgaata tgctagattg
     4321 agaactatta attctttagc atctcattta agttgttctt tccttacaaa ggtgctaaaa
     4381 ttaatttaca aatgtgaaga ttttgtacaa gagaagcaca aagacaagcc tactgcgaat
     4441 aggcttgtct ttttttcatt cattacgatt ccgtatgtgt gggttagcga caatttgttc
     4501 aattaccgta tgatatagca tcttaatttg attagattac ttgcaatgta gtttattaag
     4561 agtataatat aatcataaat gtggataatt tgagaaataa tgagtgtttt atactcaaaa
     4621 ggagtggatg aaatgaaggt caaaaatcca actgaaaaaa gcagaatcca agtcggaata
     4681 gacaagaatt taaaagagaa cgcagaaatg atattggaag agctaggttt aaatcctaca
     4741 acagcaataa caattcttta caaacaagta gtcgctagag gtgagtttcc agtagagata
     4801 aagctaagtg aagaggagaa gcaaggaatc agattgcaac agctaactaa ggacatgcca
     4861 gtagatgttc tagatacaga tgaaaagtta gaagagtggt tcgatgaagc ctaatgagat
     4921 agtaacggtc tatgttgcat ttacagataa aaatggtggt aaaagaagac ctattttggt
     4981 ggttagtgat aaagaagata gagtagaatt ttttggaatc acaagtcaat atgagaagaa
     5041 atcagaccaa ataaagagag tgtattttcc tattggagaa tgggaaaaag caggtcttaa
     5101 aaagcaatca tggattgacg tagggtcttt aaagtctatt cccaaaagcc gagagaacat
     5161 atcatttaaa aaggttggta tgctatcagc tagtgatacg aaaaagctaa ataagtttat
     5221 cgagagatta caaagaaaag tctaacaagg cttttctttt tttgcttata tgtatattaa
     5281 gatatacgtc tatacaaacg tatttatgta tataaaaata cacgtaaata tatacgtgaa
     5341 tataaacatt aagaaaatat taagaatgat taaatagtgg taattgttaa tatacgaatt
     5401 ataagatata ttttaaatcg aatgtatgta aataaataaa atgactggag gatatttaat
     5461 agtgataaat aacagtaatc cagacggata cagtgagtga agtagcttgc tacgaacgaa
     5521 caagcagaat gaaacggtag tttcattaga gcaagtcgct tgcgacgtag cgttaccgct
     5581 gatgtaactg gcaagttaca tagagagagt ttgcttgcaa acgaacgtta cctacttgaa
     5641 tagaaaacaa aaataacaga ttagtgaatt ttaaatagga gccacaactc aaccaaccgc
     5701 ttgcggtggg tgagtcccac ggaggaaaag acgaacgaac ggtcgcgtag c
//
LOCUS       DACDIP010000104         5733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDIP010000104 DACDIP010000000
VERSION     DACDIP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.105"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..293
                     /locus_tag="IV257_002610"
     CDS             <1..293
                     /locus_tag="IV257_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815692.1"
                     /translation="VLLITVKIVDYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKK
                     LKESPNEMWFEVTVESSDFKEKKKEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            618..1235
                     /locus_tag="IV257_002611"
     CDS             618..1235
                     /locus_tag="IV257_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8815693.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1276..1575
                     /locus_tag="IV257_002612"
     CDS             1276..1575
                     /locus_tag="IV257_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815694.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1609..1776
                     /locus_tag="IV257_002613"
     CDS             1609..1776
                     /locus_tag="IV257_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8815695.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1821..2426)
                     /locus_tag="IV257_002614"
     CDS             complement(1821..2426)
                     /locus_tag="IV257_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8815696.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            2551..5538
                     /locus_tag="IV257_002615"
     CDS             2551..5538
                     /locus_tag="IV257_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAP8815697.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
BASE COUNT         1959 a          946 c         1048 g         1780 t
ORIGIN      
        1 tagtactttt aataactgtc aagatagtag attactttgc gaatactcct gaagaacaaa
       61 tacagagaaa acttcctgag tttgaaagag taccacctta tgatccaaga gataattcat
      121 ttcaagtaaa aaaattaaaa gagtctccaa atgaaatgtg gtttgaagtt acagttgaat
      181 caagcgactt caaagaaaaa aagaaagaat acaattatat tgtaaaggca aaagtatcaa
      241 aaagatttat ttttaaccct aaattagaaa tatatagtgt tgtaatagaa taatagtgtt
      301 aaaaaaattg taaaagttaa agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg
      361 aagacataaa agaaaaactg gataactagt ttatttaatg gcagaccgtt aaggcctgcc
      421 atttttatgt attttagcgg aataaatata atcatgcggg agtgtaaact tactgtttca
      481 aaaagtatac tttttgacat gccttgattt tctttgttta tgtatggcat aataaccaca
      541 taatacaatt tgaaacgttt ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa
      601 aagggtagat aaaaacgatg agaaaatttt tttatatccg ggtcagtagt tatgatcaat
      661 cgatcgaacg acaactgatt tccgcgaagg aattaggaat ccctgaagaa tatatatttg
      721 tagaaaaagc cagcggcaaa gattttaaac gccctgaata tcaattaatg aaacggatgt
      781 ttcgaaaagg agatttactc tatctacaat cattagatag actaggcaga aataaacaaa
      841 tgattttaga agaatggcga gaattaacta aggaaaagca gattgatatt tctgtgttag
      901 acatgcctct gttagatact gctagttaca agcaactaga tgggttagag acgcttatct
      961 ctgacttaat tctgcaatta ctcagctata tggccgaaga cgagcggaaa cgaattaata
     1021 gccggcaaag agaaggcgta gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga
     1081 aaattgcttt acctcctgaa tttcctgcga tctattctga ctggaaagca ggaaagataa
     1141 ctgcagtcta tgctatggat caattgggca tgaaacgaaa taccttttat cgacgggtga
     1201 aagaatatga acataccttg caccactttt cttgatagat ataacagcta ttagacggga
     1261 agggagagtt tgaaaatgga cttaacgaat caaagggtcg tttttactgg aacgctgcag
     1321 caaatgacac gaacccaagc catcggtttg gttcactctt taaacggcca ttgtcaatca
     1381 agtgttactt caaaaacaaa tttcctggtt tgtggtcagt attctaagtc gttgtttgat
     1441 gattctctct atacaaaaaa ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag
     1501 attaccattt tatcagaagt cgaattttat gcgttaatta gtcaacagtt gaaagaatgg
     1561 caacggtatt tataatcgtt agtaaaattt acaaggagga aaatttttat gaagcgaaaa
     1621 tcttatgatc gttggggcat tgaaataact agttttaatc gaaatattgc ttttcacgtg
     1681 gagtgtgact gtggggaatt agcaaaaagt gtttatcaaa aaaattactt ttactgtcct
     1741 aaatgccatc gccgatacgg ggtacatgga acatagaaac gaaatagtgc ggtagaaaag
     1801 cgaatgaatg tagtatattt ctacaaatca ttcgctttct ttattttata aatagttggt
     1861 cgagagatcc cgttttcttt tgcaatctct gcaactggaa tgccttgctt taacatgtcc
     1921 accacggttt taaaaactaa gcgtttttgc ggatctgttg agttaataga atactcgatt
     1981 ggtcgtccct tatatcggcc ggctttttga gccaataata ctccttgccg ctgacgttca
     2041 cgaattttct cacgctcgtt ttgtgcaata taacttagca gtgataaaaa catatcgaac
     2101 atagcggtat ccaggagttc atttccagta ttaaaatcta aaaactgtgc atctaatatt
     2161 tttaacgcta cttttttctg tttcataaaa gcaactgtat tttttatatc ttcataatct
     2221 cttcctaaac gatctagtga tgtaacgatg acgcaatcac cttcacggat ataatttagt
     2281 aatttttgaa actcaggacg ctcagtatct ttaccactgg ctttttcttg gaataattta
     2341 tcaatgttaa gtccttccat cattgccaat tgtcgatcca aattttgatc ttgtgaagat
     2401 actcgaatat agccaatttt tcccataaaa aactcctttt taaaagtgat ataatttaca
     2461 tgtaaaattt acttgtagtt ttactgctat catagcattt ttaaaaataa attttatgta
     2521 gacttaaaat ttacttcatg gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca
     2581 tttggcattt atttaggaga acctagtaca gaacaattaa accaattttt ttatttgcat
     2641 gaaaaagatt tagaattgat ggagactata cgacgaccag ctactagatt aggttttgca
     2701 ctacagttag tctcactacg ttttttagga acttttatat tagatcttac caatattccc
     2761 caaaatgtat tgagttatgt agcaactcaa ctaaatattg atcctggaga aattatctac
     2821 tatagcagga gacagaccag gtatcagcac acaaaattga taaaatcaca ttatggatat
     2881 caagatttca accaaccgga aatagaaaca tacctagcta actggctttt aaatagagcg
     2941 ctgtatacaa cagaatcttc agatatgttg tttgatatgc ttctaaaaaa atgtctggat
     3001 gaaaagattg ttttacctgg aattactact ttttctcgtt ttgttgctag tgcaattgaa
     3061 aaagctgaag agcatttata ccgacaattg tctttggttc caactgaaga tgagagaaag
     3121 cggcttctca atcatttaga attaattgga actccagttt atggggcaat aatcaaaatg
     3181 gacattttga gaaccccgtt agctgacgat agtcataaag aaatagccag aggttttgac
     3241 cgactaaagc aatttcaagc tttctctact gaaaaatgga agatggaatc tattcctgaa
     3301 gggaaaataa aagttcttgc aaattatgca tttaaagcta aagctcaatt aattcaacga
     3361 atgagtgaac agaaaaaact ggcgttgtta gtggcattta ttttcatata caaacgtaag
     3421 gctatggatg aacaaatttt agcactatct aatttttttg aaactgtttt tagacgcgcc
     3481 aaaaataagg aatctaaaga acgtcttcgt accataaaag atctagatcg agccgcatcg
     3541 acactttcag atattgtaga tttagtaatg tccagtcatt ctgatactaa tgaactatcc
     3601 cttcgcgatc gtatcttaga aaaatattct gaggaaaatg ttaccgaagc tgtatcgcaa
     3661 gttcggctat tggttaaaaa tgatcgtgaa ccaatagcca tttctgaact tctgaacact
     3721 tatcgaaagt tcagaaaatt tattccggat attttaacta cactaacatt tgacagcaat
     3781 gactacggta acaactgtaa ggaactctgg accttcatta ataatagatt tcctaagccg
     3841 attacttaca aaatgtttgg agctattaaa catactcttc caaagaaatg gatttattac
     3901 attcatgaat accctgaaca aactaataaa tgtgtattga ttgcagctgt cgaattgtta
     3961 attcaatctt taaaacgcca tgatatttat attcctcaga gtgtcttata taacaatcct
     4021 atggattgct tgattgatga gaaaacttgg gttcaacaaa aatacgtttt attagaacaa
     4081 ctagatcttc cttcttcagc aaatgaaatg tctttatggt taaagaacga tttggaactg
     4141 tcttataaag aagcgctaaa tcgttggcct acttctaaaa tggccagaat agaaaatgca
     4201 gatcgcctgg ttgtctctag acttaaaaag acatatgaaa aaaaggaaga taagtctttt
     4261 agacgtcgca ttcagcagtt acttcctaaa attgatttgt cagatttgtt attggaagta
     4321 aaccaacagt taaatcttac tcaatcattt aaccacttaa gtgacaagga caccaaaatg
     4381 aaagacttgg atattagttt attagcagtt cttttatcag agtcctgcaa tattggtttt
     4441 tccccggttt caaaagaagg atttgattct ttaaagtatg atcgtcttgt ctatgtcaat
     4501 catcattata ttcgagtgga tacactttca ggagctaatc aaaaaataat acatgcacac
     4561 aggaaacttg ccacctctaa gatatggggt gatgggcata tggcttcagc tgacggtatt
     4621 cgttatatca caccacaacg ttccctatat tctagaagta atccaaaata ttttggccgt
     4681 ggtcgtggga tcacattcta caattttgtc tctgatcaat atatagggtt ccacggaatg
     4741 gtcgtttcag ggacactgcg agattcgctt taccttctag aaggattcct caatcaaccc
     4801 agcgaattaa ctcctaatca gatcatgact gatacagctg gttatagcga tatgattttt
     4861 ggtctctttg gcttattagg gtttcaattt agtccaagaa ttgccaatga taaaggaacc
     4921 aaactttggc gaatcgacgc taacgctcac tacggggaac ttgatacgtt gtctcaaaat
     4981 tcaattaata tggaactgat ttatacccat tgggaggaca ttcttagggt tgcaggttcg
     5041 ttgaaatctg gaaaagtgaa tgcaacagaa ttaacgaaat ctcttcaacg gaatgggcaa
     5101 cccacgcctt taggaaaagc aataactgag tatggaaaag tttataagac gaaacatcag
     5161 cttcgttata tttctgacga aatatatgca cggcaaattt tagaacaatt gaataagggg
     5221 gaagctcggc atgccttatg ccgtaatatt ttttatggac gaaaaggaaa actctaccag
     5281 acttattatg atgggatgga agagcagctc aatgcattag gattagttac taatgccatc
     5341 atatattgga actcactgta tttagaaaaa gtatttgaac aaatgaaagc tgaaggattc
     5401 gactgttccg aagaaatgat acgcaggcaa tctccactaa ttagtgatca catcaatttt
     5461 gttggtaagt atacgtttag atacgaagct gaattatcca acggtaagtt acgtccattg
     5521 aatgtagaag aattctagca atgttcgggt tttgcatgct accgcactat ttcgtatgac
     5581 tgttccagcg aatcacttcg aatagaaaaa gagaaaaaca cgagaacagc gttaaatcaa
     5641 cgtttcttgt gtttttactt ttgtgctacc gcactatttc gtttctatgt tccatgtacc
     5701 cctttatcaa gaagcgcaaa cacgccaaat tgc
//
LOCUS       DACDIP010000105         5727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDIP010000105 DACDIP010000000
VERSION     DACDIP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.106"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1052
                     /locus_tag="IV257_002616"
     CDS             <1..1052
                     /locus_tag="IV257_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP8815698.1"
                     /translation="TGANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQ
                     SGSIKDYKGTPRTVLVQRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPL
                     GVRKMTLIDNDCFQAENLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEV
                     ESMNVLDLISMQPGFFKEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGG
                     HTLFINYSLNQKGCFNCLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAI
                     ASTECAINAIKLFHKVNQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEE
                     RFFSKYCKVCGELDD"
     gene            1045..1506
                     /locus_tag="IV257_002617"
     CDS             1045..1506
                     /locus_tag="IV257_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815699.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            1519..2379
                     /locus_tag="IV257_002618"
     CDS             1519..2379
                     /locus_tag="IV257_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815700.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            2417..2806
                     /locus_tag="IV257_002619"
     CDS             2417..2806
                     /locus_tag="IV257_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8815701.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            2793..5240
                     /locus_tag="IV257_002620"
     CDS             2793..5240
                     /locus_tag="IV257_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8815702.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            5197..>5727
                     /locus_tag="IV257_002621"
     CDS             5197..>5727
                     /locus_tag="IV257_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8815703.1"
                     /translation="MLLIPVRQFKREWSEEMKKKLFRIFGIVLISLVTLIILLSLVGT
                     VAEATGLVDDTVGAGNLYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAI
                     TNFIWTVSLYLSNATGYVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFG
                     FLLLMILALGVYVAYTG"
BASE COUNT         1858 a          869 c         1196 g         1804 t
ORIGIN      
        1 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
       61 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
      121 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
      181 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
      241 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
      301 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
      361 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
      421 ttaaagttga tttaatgcag gaggagctcg aaaaaaaatt cccatacctt tgtgttgaag
      481 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
      541 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
      601 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
      661 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
      721 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
      781 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
      841 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
      901 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
      961 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     1021 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     1081 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     1141 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     1201 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     1261 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     1321 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     1381 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     1441 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     1501 ctataaagga gtgattagtt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     1561 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     1621 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     1681 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     1741 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     1801 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     1861 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     1921 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     1981 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     2041 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     2101 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     2161 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     2221 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     2281 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     2341 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     2401 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     2461 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     2521 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     2581 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     2641 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     2701 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     2761 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     2821 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     2881 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     2941 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     3001 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     3061 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     3121 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     3181 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     3241 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     3301 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     3361 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     3421 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     3481 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     3541 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     3601 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     3661 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     3721 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     3781 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     3841 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     3901 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     3961 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     4021 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     4081 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     4141 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     4201 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     4261 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     4321 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     4381 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     4441 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     4501 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     4561 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     4621 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     4681 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     4741 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     4801 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     4861 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     4921 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     4981 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     5041 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     5101 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     5161 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     5221 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     5281 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     5341 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     5401 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     5461 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     5521 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     5581 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     5641 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     5701 ctaggagttt atgtggcgta tacagga
//
LOCUS       DACDIP010000106         5699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDIP010000106 DACDIP010000000
VERSION     DACDIP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.107"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IV257_002622"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IV257_002622"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IV257_002623"
     tRNA            203..276
                     /locus_tag="IV257_002623"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IV257_002624"
     tRNA            316..405
                     /locus_tag="IV257_002624"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IV257_002625"
     tRNA            420..491
                     /locus_tag="IV257_002625"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IV257_002626"
     tRNA            507..579
                     /locus_tag="IV257_002626"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IV257_002627"
     tRNA            589..661
                     /locus_tag="IV257_002627"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IV257_002628"
     tRNA            697..772
                     /locus_tag="IV257_002628"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IV257_002629"
     tRNA            774..854
                     /locus_tag="IV257_002629"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IV257_002630"
     tRNA            861..934
                     /locus_tag="IV257_002630"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="IV257_002631"
     tRNA            937..1009
                     /locus_tag="IV257_002631"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="IV257_002632"
     tRNA            1021..1092
                     /locus_tag="IV257_002632"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="IV257_002633"
     tRNA            1138..1208
                     /locus_tag="IV257_002633"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="IV257_002634"
     tRNA            1229..1313
                     /locus_tag="IV257_002634"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
     gene            1375..1447
                     /locus_tag="IV257_002635"
     tRNA            1375..1447
                     /locus_tag="IV257_002635"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:His,seq:gtg)
     gene            1457..1528
                     /locus_tag="IV257_002636"
     tRNA            1457..1528
                     /locus_tag="IV257_002636"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Gln,seq:ttg)
     gene            1546..1630
                     /locus_tag="IV257_002637"
     tRNA            1546..1630
                     /locus_tag="IV257_002637"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1580..1582,aa:Leu,seq:caa)
     gene            1885..2760
                     /locus_tag="IV257_002638"
     CDS             1885..2760
                     /locus_tag="IV257_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8815704.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2864..3628
                     /locus_tag="IV257_002639"
     CDS             2864..3628
                     /locus_tag="IV257_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8815705.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3645..5057
                     /locus_tag="IV257_002640"
     CDS             3645..5057
                     /locus_tag="IV257_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8815706.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            5074..>5699
                     /locus_tag="IV257_002641"
     CDS             5074..>5699
                     /locus_tag="IV257_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8815707.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIY"
BASE COUNT         1720 a          965 c         1287 g         1727 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tttattgaga aattaaataa catcttttat gatggcgaat
     1381 gtggcgaagt ggttaacgca ccggattgtg gctccggcat tcgtgggttc gattcccatc
     1441 attcgccctt tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     1501 tgcgttggtt cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa
     1561 ttggcagacg cgctggactc aaaatccagt gtccgttgag gacgtgccgg ttcgaccccg
     1621 gccgccggta taattatcaa tatagcaact ttcttataga gagttgctat attttttttg
     1681 tttttatgta attacctcca aatcaattcg atacaagtat tatcataaaa cgagtaaaaa
     1741 agatattgat aacgctttta ttatgtgtta aactattttt gtaaattatg tagggtgcta
     1801 ctattcaatt aggcacgacc taagaaaggg ttctttctta ggtcgttttt tgtctttaaa
     1861 aattgtttgg agacaaaaat atgtatgaag gagtctgcaa gttgttttgt tttatcatat
     1921 agaggaggtg aattaacggt gtatgtgatg aaaaagctga atcataatgc tgtattgatc
     1981 gatgacgatg gaatagaaaa aattgcgatg gggaaaggga ttggcttttc tgctaaagtg
     2041 agtaagccat ttgatgttga ggatgctgac aaattatttg tattagattc taaagaaaag
     2101 atcaaacgat tcagtgagat agtctcgcag attccaatcg aatttatgga ttttgcggaa
     2161 gaagtgattg aactgataac agctaggatc tcctctccat tagattcaaa tatctatatt
     2221 acattgacag atcatattta ttttgctatt caacgaaaaa gagaagatag agatgtcaca
     2281 gcaattatgt tgccagaaat gagattcctt tatccaaatg aatatgatat agcagtagaa
     2341 gtggtcgagc gaatcaatca tcgatatcaa accgagttag ggtcaaatga agtaggattc
     2401 atcactatgc atatcattaa tgctgagcta ggagagccta atagtttaaa gagcttaaaa
     2461 atagtagaaa tgacgaccac gattttgtcc catctagaaa aatcctattc aaacgtttat
     2521 gataaagaat cactgactta tcatcgctta atggttcata tcaaattcct ggttaaacgc
     2581 ttgatttatc aggaagaaac gccagtagaa aatattggtt tttttactga aacatttaaa
     2641 catagcaaag cgtattgtac agcactaaag attaaagata tcatcgaaca gcaatttcaa
     2701 cttgccattc aagaaaatga aatcatctat ttggcaattc atctagcaag aatctattaa
     2761 aataagggcg taactatcta taggcgcaac caaatgacac atagtaaatg gactatgatt
     2821 atttggttgc gcctatttta gtagaaaaaa gaaggagaat gaaatgggaa aagtgatttt
     2881 taattcggat gattttggct atagccatgg tgtgaattat gggattatgg atgcttatca
     2941 gcgaggaatt ttgacatcaa ctacattgat ggcaaatatg ccgggtttcg agcatgctgt
     3001 gaaactaaga aaggaaatgc ctaggctagg tgtaggagtc catttaacat tgacgtgtgg
     3061 caaaccactc ctcaaaactg tcgatactct tatcgaaagc ggagagttca gatccttatc
     3121 ttattatcag catccattta acattgacaa agatcaactg tatcaggaat ggaatgctca
     3181 gattcaaaaa atttatcatg cggggatcat accaactcat ttagacagtc atcatcatac
     3241 tcatactttg gatgaaaatc aggaggtagt tgttacatta gcaaaaaaat atgatttgcc
     3301 ggttcggagc aactttgaac gaaaagatga agttcgtcat gtcgattatt ttgagccttg
     3361 tttcgatgtc gtaggagaaa ctgatgaaaa aaaacgacaa atcaatacga gtgtggaaga
     3421 atatttggca agtttactca tgatattgag agaagataaa acgatcgaag tcatgtgtca
     3481 cgcagcgtat ttggatcagg aattgttaaa tggttcaagt tttgtgtatc ctcgtaccca
     3541 ccaagtcgaa tttttaattc attcagcatt tgctcaaaaa gtgaaaaatg atcctttgat
     3601 tgagttagta acatatgagg atatttaata gaggaggaat aaaaatgaat gttaaatcaa
     3661 atgtgcagaa gcttggaaga gcgatgctac ttccagttgc agcgatgcca ttagcgggat
     3721 tgatcatgcg tttatcagct gacgatatgc tgaacatccc agtaattggt gcagctggaa
     3781 atgccgtttt tggaaacttg gatatcttat ttgctatagg agttgcaatc ggttttgtca
     3841 aaaataagga caaaggaata cctgcactta ctggtttttt agcgattgct accttgaaaa
     3901 aaggcttaga aattatggac ccagaagtga atatggggat ttttggtgga attatttccg
     3961 gtttagtagc ggcatggacg tataatcgct ttaaagaaca aaaattacca gtagtttttt
     4021 cttattttgc gggcgaaaaa ttcccattga ctatggtgat gattctacaa acaatcgcag
     4081 ctgttttttt gggctttatc tggccgctca tccaaattgg gattgataat tttgcaaaac
     4141 tgctcgttga tatgggagca ttaggtgtgg ggatatttat gtttttgaat cgtttgctga
     4201 tcccctttgg actgcaccat gtgttgaata cgtatattta ttacgattta ggaagctata
     4261 catctcctag caaggaagtt ttcagaggag agatgacacg ctttatcaac ggagatcctc
     4321 aggctggact gtttttatca ggtttttttg tagtaatgat gtttggtata ccagctatat
     4381 gtctagctat ctataaagca gcttataaag aaaataaaga gatggtcaaa ggaatcatgg
     4441 gaagcggtgc aggaacttca tttattgcaa atatcactga gcctgttgaa tttagtttca
     4501 tgtttctttc tcctatgttg tacgtgatcc atgcactttt tgccggttta gcagggattg
     4561 tctgttactt attcaatata cgaattggtt ttacattcgg agcatgtatt gttgattatc
     4621 tcatcaattt tcgaatcgct accaatgcta ttttgattct tccaatcgga attttctttt
     4681 ttgccttgta ttacgttact ttttattatt tgatcaataa gagaaacatt cagacattag
     4741 gacgtgaagc taaggcagag tttggcaatg aagtaacctt agaagaaaca gagttagggt
     4801 tagcaagtaa aaattatgaa tatatggcaa caaaaatgct acaagcgttt ggaggaaaag
     4861 ccaatatcct ggatgtctat agttgtaata cacgcctacg ggtagaggta gtggatccca
     4921 ccatggtcga ggaacagcga atcaagcaat tagggataag cgggatcatt aaaccaactg
     4981 aaaaaaatta tcaaattatc atcggcttag aagtgactta tgttatggca gaattcaata
     5041 aattattaga agaatagaaa ggaaaagatg atgatgaaaa aacagattat cactatagct
     5101 ggaggcggga gtacgtatac accaggaatc gttcaagcca ttttgaacaa tgaaaaacgg
     5161 ttgccattat ctgaaatacg tttatatgat attgatgaag aaagaaatat ggatatgtac
     5221 ttgattgtta aatttatgct gaaaagaaaa ggtttttcaa atattcgcat tcgtgcaacg
     5281 gatgatccaa agcttgcttt tacaggatgc gattttgttt tctcacaaat tcgtgttgga
     5341 ggattggaga tgcgagaaaa ggacgaaaag attccactta aacatggatt agtaggacaa
     5401 gaaacgtgtg gactcggagg atttgcttat gggatgcgct caatcaaagg tttattggaa
     5461 attgtcgatc atatacaaga ctatgccccc aaagcatgga ttttgaatta tacaaatcca
     5521 gagtcgatcg tttctgaagc tgttcgtcgt aaatatccga atgtaaaaat gattaatgcc
     5581 tgtgatatga ctatttcaat tgaagaaacg attgcagtga attacggtta tgatcgtaag
     5641 aattggatcg ttacgtatta tggtttaaac cactttggct ggtatacatc gatttatga
//
LOCUS       DACDIP010000107         5644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDIP010000107 DACDIP010000000
VERSION     DACDIP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.108"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..878)
                     /locus_tag="IV257_002642"
     CDS             complement(<1..878)
                     /locus_tag="IV257_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815708.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQH"
     gene            1000..1584
                     /locus_tag="IV257_002643"
     CDS             1000..1584
                     /locus_tag="IV257_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815709.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            1595..1840
                     /locus_tag="IV257_002644"
     CDS             1595..1840
                     /locus_tag="IV257_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815710.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1846..2568
                     /locus_tag="IV257_002645"
     CDS             1846..2568
                     /locus_tag="IV257_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815711.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            2699..3442
                     /locus_tag="IV257_002646"
     CDS             2699..3442
                     /locus_tag="IV257_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815712.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            3448..3813
                     /locus_tag="IV257_002647"
     CDS             3448..3813
                     /locus_tag="IV257_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815713.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3829..4359
                     /locus_tag="IV257_002648"
     CDS             3829..4359
                     /locus_tag="IV257_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815714.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            4521..4751
                     /locus_tag="IV257_002649"
     CDS             4521..4751
                     /locus_tag="IV257_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815715.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVRLPVSELKK"
     gene            complement(4955..5434)
                     /locus_tag="IV257_002650"
     CDS             complement(4955..5434)
                     /locus_tag="IV257_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815716.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
BASE COUNT         2053 a          746 c         1000 g         1845 t
ORIGIN      
        1 tcatgttgta attctggatg ggtagctata aaagtaagta aaaagaaccc ttttgtatac
       61 gtgatctgtt gattttcacc aagtgattta ttaatttcct ctcttgattc agctgtgaac
      121 ctatactttg caatttcttt ttttagaata tttaaataga attctttatt atcaaaaatt
      181 agggcattaa gtagtctgaa agtactttcc ctatttctat ctgcttcctc atttccataa
      241 gataattgaa aaacaaactt aaaggctgtc ttacataggt tttctttggc tttttcgctt
      301 agtttattta gatagctatt ttttagtgca gagtgaaatt cttcaggtgt aacagacata
      361 gcatgtatgc gacttgcata ttgtataatt tcgatcgtta ctttttttcc taatattgga
      421 ttttttagga atataatttc ataggacttc cgtataacat cctgacaagt ttcttttgtt
      481 atatctctta attcccaagt attaccgttg taagtgacta caggatgagc tgaataattt
      541 ctttgatcct taatatattt taaatcctca tattcatagc tatcaattag ctcagttgaa
      601 ctataagctt tttctagtag ttccatttcc cattttggag agtttggatc actttttctt
      661 agtctagcta tatcttctaa aattttctta gaagtattat cctcgtaaat atttgctaaa
      721 tcagataatt tatcaattaa atctaaaata agcgcactgt aattgacagc tattgcagaa
      781 cgataatttc cctgataaaa actacttcta acttcttcta ataatggaat aattctagga
      841 tttttaactt tttttatttc atcatctaat ataaacatat aaatctcctt tttttatttg
      901 tatgacaagt ttaccataaa aaaaaattaa aaagcgagac atatttatgt tgttgtgtta
      961 tcataataac ataaatatgt agagtgagat gatgaaaaaa tggatttgaa acaatcgttt
     1021 tacatctggt tatatcctga taatcctatg tcaataatgg ctgatatttt tgtagttagc
     1081 atcttattgt atttatttta tctaattttc tttcacaatt taatcgggta catgattctt
     1141 gattggaaag gaatgttcga tatagaaaga aaaccatttg ctaacaatca aacctatacc
     1201 gtcttcgaaa gtggagattg ggaagaaaat gaagttgatg tgatccatga aattggatta
     1261 gctttatcag gaatacaaga tgctgatgtt ttggtatcaa tgacaacgga tactggatac
     1321 aacgcttatg aggtatttta tcttatgtat tttatatggc gtgatggtca agaaattatc
     1381 aatggtcgtg ctgaaaaaac aatcaattct aatactgaaa tttctttttg tatgggtgaa
     1441 ggggaaatga ttgtatataa agatgggtca gcaaacatta aaacagcctt tggtgatatt
     1501 gaaacaagta ttaaggaact tagggaatat gtgcagaacc catataattt tcaatattta
     1561 gcaggaagaa taaatttagt ttaggagtaa aaatatgaaa aaaattataa atcaaccaac
     1621 aaatcacttg ataattgaag gtgttatttt cgtaacaatg tattttactg taggaaatgg
     1681 catctcaaat acaatcattc aaaaaaatcc acaataccta ttatttagtc tttatgcttg
     1741 gattgtacaa atggtttggg tttccatatt gatgctgttg ttttattgga tagtacgggt
     1801 tattctttta ttgttaaaca aaagaaaaaa ggataggtga ataaaatgaa atcaactaaa
     1861 ttgaaggttg tattgttatc gacagcagca ttaataaccc ttacagcatg tggtacaact
     1921 aatagtgcta ataataacaa agacacaaag tctagtagta cgatggttac taaaaacagc
     1981 actacatcgg catcaacaac aatgtcttca atgactgaaa ataatgctga aaagtatcaa
     2041 aaatacgaag acattaataa aggtcaaaag ccggatgaag tttataacaa ctttattaat
     2101 acagtaaaaa atgatgaaaa ttatgggaat gatccattgc aaaataaaaa tctgactatc
     2161 gttctttaca aacctgattg tccagattgt caaaaagcag aatcaaaaat catgcaagaa
     2221 ataaaagaag gatttagtta tgctccaaca ttaatgcatg atattaccat aaataaacca
     2281 gcttacgagt attacgcagt tgctgtagat attactgatg gtgtacctga gtgggtacaa
     2341 aacattcctg gtctggctga tgagaacaat caatatcata cgccaacagt tgcagtagta
     2401 acaccaaaga aagttaacgg cacactatac tgggatgtaa tgggaatgac aacaaataca
     2461 actgatgaaa tgattgaaag tacaactaaa tattatatgg atttgggagc atataaatta
     2521 tatcaacaat cagagcctgt tatggacgat caatccaaag ttaaatagat ctttagggcg
     2581 taagccctat ttttttaaaa ttaagtgata acaaataaat gatttctgaa acaaacgttc
     2641 ttactcaact actcgaaaca cacaagctaa ttaactagat tataaaggag aatgcgtaat
     2701 gaaagaaatc agtgcaaatg aagctcatat tccgcaaaag aatcaggttg gtatatataa
     2761 ggtgcttcat ggtgaaggaa tttataaaat acagttactt accggttaca ataatggtgc
     2821 aaatgggaag tggttaaagg caacaagtat tcgtgaactt agaagtgaag tagaaaaaat
     2881 taggcttggt ctcacaagtg aagaaatagc taaactgaag ggaaataatt cttttgtaat
     2941 tgaaaaagaa aaggaaaaac atctacttta tcgtgagctg aaaattgaag aatactatga
     3001 aattgattta cctgaaaaat taagaattat tgatcaacgt tcagaagaaa aatggaaaac
     3061 tgaaatatat ccaagcttaa atgactacaa gacatcagga aagcccatgt ataactacac
     3121 aggcttttta acctacacac aatacaaata tgcctttcgc aaattaaaag gcattaaaca
     3181 agctgaaatt gctctgtatg agatgaaaaa agtggataga ttgaaattga ttcaagattt
     3241 agtgcaaaat acatggaatc aagcgattgt cgagctagaa aaagaaaatg ggaaaatttt
     3301 aactaataaa tatctgatcg taaatgaaat tgaagaatta attggtttat tttcatgtga
     3361 aaaaggtgaa gtcttcttga agtcatcacc aacctattca attttggaca gtccgtatca
     3421 gtttatacag gcaaggagat gagttagatg tctagcttga aagatgacta tatccaagca
     3481 aaacaagagt atgaaaaaat acagatgttg aaatctgaga aagtcgaatt atttttaaca
     3541 caaaaattag cagaggatca tattgctaac ttagatgcta aaactttaga ggaatatgat
     3601 cggcagttta atcagataca tcacaatatc attttgcttg ttgagcaggc aaagcgaagg
     3661 tttgaaaaag cggaatatga tctattagat caaggaattg tatgcttgga taagggattg
     3721 cgaagatatt atggtgcaga gttaaaaaag caaaatgtag atagaaatgc actaaaacat
     3781 gaaatcttag aattgataat gcaacgaatt tgaagggata gaaagcaaat gtcacaaaac
     3841 attagtgctg taaaacaatt aatagaagaa gatttagaga ccacagttca gagattagcg
     3901 gaacgaatcc attttgcaaa ttatcaaacc atctctaact ggtcaagcag gaacagaaat
     3961 ccggaagaaa ttccaatgaa atttttttat cgaatcggaa aagaatatgg tgttgatccg
     4021 ataaaaattt tcgagaagac catgatttat tactatcatg gaacacctaa cgaagtgtta
     4081 gaacatcttt tggaagaggc aaaagaaaaa tcatcaatat tagagctatc gcaaaatgac
     4141 aaagttattc aagtgaaatg cgttgatcca acaggaaaga ttattgtcgg ttttgatatt
     4201 gaaagaaaat ccaaaataga ttttattaca caattaaaaa agataaataa tttagatatt
     4261 ctaacgctta gaccagatca tgaacagttg tttatgagtt ttaaaaaaca attgaaagaa
     4321 atacaaaaaa gatataacaa ggtgtttcct aatgcgtgaa aagctcaaag aaatcagagt
     4381 tttttggata attaacaaag aatgtatcat cttatatact cttggatttc ttagcatgat
     4441 cttgttttgg tggttatatt taaaacagta attggagagg gtcaagagga tttttatcaa
     4501 gataaggatg tggaaactgc atgtggaata atagattaaa aacaggattg ttattaatag
     4561 ttatttcctg cgctatgatg attggtatga ggatacaaag ggaacaatcc tattttgaag
     4621 tttctgctaa caacgtaata gaaaaatgct attacggtca acattattgg agtgaggaag
     4681 tcagagaaaa tatagatcgt gaatatgttc aaagaattgt tagattacca gtaagtgaac
     4741 taaaaaaatg atgagattat cagcatatct tttaatgttt ttgaatgtgg tcacgttaat
     4801 ctttgtagta atcggtgtga ctttcgattt aatggaatag tgggaaatct ataatggaaa
     4861 aaagagacac gaatgaacat attttttgaa tcaatattgt tgatggtttt tgcgatcagc
     4921 gcttaccaga aatctaaaaa agagtaatga ttgtttaatc atcactcttt ttttgtaggg
     4981 aaattgaaat atgtggagct tgtattggtt tagttaggat tgcttggcta taaggagcta
     5041 gttctttatc aaaaccatca aagtaggttt tcagtagcga tttaactgat cgctcatctt
     5101 gtttgatttg gataatttca gcatccaaat cggttgtgag atcaggagct tttgatgcga
     5161 ttacctgatt ttttttatct tccaactctc ttaggctgtt taacaagttg taaaaagaat
     5221 cattttgatt gttcattcca acgtgagttg caattttaac agaagtttcg tccgtattgt
     5281 catcaaaaga actcattgga atatcaagta gtaacgagga tgtttctttt actgactttt
     5341 tctttacagc attttttgtt tttccatctt ttttgctctt tactcgagat gtttttggag
     5401 ctagtttagg taaatcactt gttttatcag tcatttatta gcttttcctt tctattaatt
     5461 tatgttatta tattatcata ataacataaa aaagtacact ttttgggggc gtagttattg
     5521 aaaaaatcta agagaaagat tgccacattg cttatgttaa tggtgcaatt tacagggatt
     5581 gcaatgagtg taatcacaat aaatccgcaa gtgatacatg ctgatgggat agctatttat
     5641 gatg
//
LOCUS       DACDIP010000108         5397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDIP010000108 DACDIP010000000
VERSION     DACDIP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.109"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            33..1544
                     /gene="gadC"
                     /locus_tag="IV257_002651"
     CDS             33..1544
                     /gene="gadC"
                     /locus_tag="IV257_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP8815717.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1557..2957
                     /locus_tag="IV257_002652"
     CDS             1557..2957
                     /locus_tag="IV257_002652"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP8815718.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3122..4557)
                     /locus_tag="IV257_002653"
                     /pseudo
     CDS             complement(3122..4557)
                     /locus_tag="IV257_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4950..>5397
                     /locus_tag="IV257_002654"
     CDS             4950..>5397
                     /locus_tag="IV257_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP8815719.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         1718 a          873 c         1026 g         1780 t
ORIGIN      
        1 tgacaaatta aataggaaag ggaagtaata atatgaataa aaaaaagtta tctctattcg
       61 gtttttttgc cctgacagct tccatggttt tgactgttta tgagtatccg acctttgcaa
      121 catcacaatt acatttggtt ttctttcttc ttttgggagg tattttatgg tttctaccag
      181 ttgctttatg tgctgcagaa atggcaacag ttgaaggatg gaaagatggt gggattttca
      241 gttgggttag tcaaacttta ggagaacgtt ttggttttgc agctattttt ttccaatggt
      301 ttcaaattac agtaggtttt attacaatga tctacttcat actaggagcg ctgtcgtatg
      361 taattaattt tccagagctt aatacagatc catttatcaa gtttattggt gtattagtca
      421 ttttctgggt actgactttt tcacagttgg gcgggaccaa tcgtaccgct aaaattgcta
      481 aagcaggatt tgtcattgga attgttattc cttcgatcat tctctttggt ttagcagcag
      541 cctactttat cggcggtaat ccgattcaaa tggctattaa tgcgaaagct tttattcctg
      601 acttttctca gctttcaact ttagtagtat ttgtatcttt tatattggct tatatgggcg
      661 tagaagcttc agcatcacat attaatgagc tggaaaatcc ccaaagaaac tacccattgg
      721 caatgttttt actagtgatt ttggccatcg ccttagatgc aatcggcggt ttctcagtag
      781 cagcggttat tccacaacat gacttgtctc tcagtgctgg tgtaatccaa acatttgaaa
      841 cgcttatttt acattttaac agtcatttac attggttggt aaaagtgatt gctctcatga
      901 ttgcctgtgg cgttatgggt gaagttagtt cttgggtagt tggtccatca cgagggatgt
      961 ttgcagctgc tcagcgaggt ctgttaccag ttttcttccg gaaaactaat aaacaagacg
     1021 ttccagttcc gttgattatg atacaaggaa ttatcgttac tatttgggca gcagtcttaa
     1081 cttttggtgg aggaggcaac aatttatcat tcttagtagc aatctcctta actgttgtca
     1141 tttatctcgt aggatattta ctatttttcc taggttactt tgtattagtt tttaagaaaa
     1201 aagacttaaa acgaacttac aatgtaccag gaaaaactgt tggcaaaacg attatcggtg
     1261 gaattggttt tattttatca gtgttcgcat tgcttatctc ttttgtacca ccagcttcaa
     1321 ttgcagctag tgagcaccga acataccaaa tcatactact tattagcttt gtagtgactg
     1381 ttcttttacc ttttatcatt tatgaagtac atgataaaag tgctcacaca actattgagg
     1441 aacctactca cgtgaaagct gaagacgtta atcccgctgt ttatccagct gctagaggag
     1501 agcaccacgt tacaaaaaat gatgaacata ttttaaaaca ttaggaggaa ttcattatgt
     1561 tatacggaaa agataatcaa gaagaaaaaa actatttgga accaattttt ggctctgcaa
     1621 gtgaggatgt tgacttgcct aaatataagt taaacaaaga atccattgaa ccacgaattg
     1681 cttatcaatt agtacaagac gagatgttgg atgaaggaaa tgcgcgatta aacttagcta
     1741 ctttttgtca aacgtatatg gaacctgaag cagtgaaatt gatgacccaa acgttagaaa
     1801 aaaatgcaat tgataaatca gaatacccac gaacaacgga aattgaaaac cgctgtgtaa
     1861 atatgattgc tgatttatgg catgctccaa ataatgaaaa attcatggga acttcaacga
     1921 tcggctcttc agaagcctgc atgctgggtg gtatggccat gaaatttgct tggcgtaaac
     1981 gtgctgaaaa attaggtctt gatattcaag caaaaaaacc taacctggtg atctcttctg
     2041 gttaccaagt ttgttgggaa aaattctgtg tatattggga tgtggaactg agagaagtcc
     2101 caatggatga aaaacatatg tcaattaatc tagatactgt catggattat gtggatgagt
     2161 acacaattgg tattgtaggt attatgggta ttacttacac tggtcgttat gatgatatca
     2221 agggtctgaa tgatttagtt gaagctcaca ataaacaaac tgactataaa gtatacattc
     2281 atgttgacgc tgcatcgggt ggcttttatg caccatttac tgaacctgat ctagtttggg
     2341 attttcaatt gaaaaatgtt atctcaatta attcttcagg tcacaaatat ggtttggtat
     2401 atccaggtgt gggttgggtc ttatggcgtg accaacaata cttaccagaa gaattagtat
     2461 ttaaagttag ttacttaggt ggagaaatgc caactatggc tatcaacttc tctcatagtg
     2521 cagcacaact aattgggcaa tactacaact ttgtacgcta tggctttgat ggttatcgtg
     2581 atattcacca aagaactcat gatgttgctg tttatttagc caaagagatc gaaaaaactg
     2641 gtatttttga aatcattaat gatggatcag aattgccagt tgtgtgctat aagctgaaag
     2701 aagatcccaa tcgcgaatgg acactatatg atttatctga tcgtctgtta atgaagggat
     2761 ggcaagtccc agcctaccca ttacctaaag acttggatca attaattatt caacgcttag
     2821 ttgttcgagc agactttgga atgaacatgg ctggtgatta tgtacaagat atgaaccaag
     2881 caattgaaga gttgaataaa gctcatattg tttatcataa aaaacaggat gtgaaaacgt
     2941 acggctttac tcactaaaat tcttcagaga aatataaaag aagttaatta actaataaaa
     3001 ggagttgtga ttaccacaca gctccttaaa tctatttgaa taaatatatg atattcattt
     3061 gttacactct caatcttagc tgttgttcta ttcatcatta ctcttattgt aagtagttca
     3121 gtcaaatttg cttgttcaat tgatcgataa cagtatgaat tgtttgaaaa gtaatcggat
     3181 atataattgt tttctcacta cctaagcctt cttggtataa agaactatcg caaagtataa
     3241 tccgctgagt acaaaagaaa gtgtcgtcta ctatttctct aagttgttta caaatgatat
     3301 tactaataga aaagtgttct cccaaaatgt cttctaactg ttgtcgatag tataaatacc
     3361 taaagtctga cggagctact atgaacactt caattggaga tttccgttta tgctcattta
     3421 aaagacttaa actaatggtt agtttacgaa tagaattctc attccaacgc agaaataaat
     3481 tgttttgttt tcgccattgt tcaagaattt catgaacttc taagtacaaa gcgcgttgct
     3541 cttcatttaa caagtaagtc ttatcaggtt gaaatatctg aatatcagcc caaagtgagc
     3601 ggataaacgg gagaagtgct ttatcaaaaa gtatctgatt ttcttcagat atattcagag
     3661 ataagataag tttttgactg agctcaatca ccaaagggtt atctgctaca tggttttgat
     3721 aaacaacgtt caagtctttt tgtaacaatt caaagttatc gcatagataa tttctagagt
     3781 tgaatagaaa gtagatataa aggcattcat cttcgtcatg acataaagag aaagaaagtt
     3841 gctctaatcc tttctgtatt agctgaaaca agggagtatg tcgggcaact cttttttctt
     3901 cctgactaaa atatattggg ttagtcttag atctctgcaa accgatagcg atacctaata
     3961 aaataaattg tttttcaact gtagggtaac ttcttgaaaa aaatgttttt ctacataatc
     4021 aatcaattgt tgaccgcttt gaataattga tttatttagc tggatcaact ctgtccgatt
     4081 tgtctcatag attatcgtta gtaacaaact tcgataatcc ttttcaggaa tttcaaaatg
     4141 gccatcagca tttaagtact gcatttgttt aaaaaaatta atcaactcat ttcgtaagtt
     4201 gtaaactttg cttaacgaaa tgaaggtagt atcagctata ttactccaat tgtgttcccc
     4261 tgataaataa tgtcctagta cattgaaaaa gtcagagcgc tgatagatga gttgagtgag
     4321 tgccaacctc gaaaacgaag agtcgaatgc gactgtaaag ctacttctag atttctccac
     4381 tgaaataatt tgctgatgga gtatgtcggt aatgcattcc aagtcgttag tgacagtgag
     4441 tgggcaaacg ttcaattgat ctgctatttc gcgaatctga atggtccctt tttcgtgtaa
     4501 ttgctcacaa atgtaaactt gacgaaaaat acttttctcc agataggttt ccaacatagt
     4561 actcctccta gtcattttat acataactat tataactcta ccaatcggaa tagtcctgta
     4621 atatattgaa tcaaaaatat aaattataaa ataattagat ggggtatttg gtttaagtat
     4681 cccttatata gtaataaatt tataggtgtg attctcgaat taatacccga aaatgtgaga
     4741 gaatttttat ggatttttta ggatacaaat taattatttt tgcttttatc ctgattacca
     4801 gtccagtact gtcggtatcc cattttgcag caaaatcatg ttgataaagt gacaagaaga
     4861 ctgatgagat atattatcca aattgagaag ataattaatc ggattcttga gtatacccta
     4921 ctttttaaga aagtagggta tacaagcgta tgaataaaga cataactttc cctgtgatgg
     4981 acgttcggct tgttccaatt accaaaatac aggcgaacga ttataatccc aatcgagtag
     5041 cgtctccgga aatgaactta ttggagcttt ctatcattga agatggcttt actcaaccat
     5101 tagtttgtta ttacaaggaa aaagaagaga agtatatctt ggttgatgtg cttcataggt
     5161 atcgtgttgc taaggaaaga ctcaacttaa aagttgtacc ggtaacagtt attgataaac
     5221 ccatagaaga acgtatggct tcaacaattc gacacaatcg tgcaagaggg acccatgaaa
     5281 taagaggaat ggaaattgtc atccaaagat tagtaaaaaa tggatggtca gacaagagaa
     5341 tatccaaaga attaggcatg gacattgaag aaatttttcg ctttaagcaa gtgtctg
//
LOCUS       DACDIP010000109         5327 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDIP010000109 DACDIP010000000
VERSION     DACDIP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.110"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..159)
                     /locus_tag="IV257_002655"
     CDS             complement(<1..159)
                     /locus_tag="IV257_002655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815720.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDS"
     gene            complement(460..1284)
                     /locus_tag="IV257_002656"
     CDS             complement(460..1284)
                     /locus_tag="IV257_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815721.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(1281..2036)
                     /locus_tag="IV257_002657"
     CDS             complement(1281..2036)
                     /locus_tag="IV257_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8815722.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(2049..4025)
                     /locus_tag="IV257_002658"
     CDS             complement(2049..4025)
                     /locus_tag="IV257_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8815723.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(4078..4749)
                     /locus_tag="IV257_002659"
     CDS             complement(4078..4749)
                     /locus_tag="IV257_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8815724.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(4809..5018)
                     /locus_tag="IV257_002660"
     CDS             complement(4809..5018)
                     /locus_tag="IV257_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815725.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(5085..5324)
                     /locus_tag="IV257_002661"
     CDS             complement(5085..5324)
                     /locus_tag="IV257_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815726.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         1469 a         1108 c          793 g         1957 t
ORIGIN      
        1 actatcatga taaagcacca gattattatt ttctttgatt tgactcggat caactttttc
       61 taaggcctgg actgcagttc ccctcgcaaa aagaagaagg aacaaaaaca acactaaagt
      121 aattactctt cccgtagtgc aatgaatttt attttccata atgccctcca tctaatattt
      181 ttttaaacga tcgtaagctt ttcattcttt ttttcctaat acccacgtta ccaatcctgt
      241 aagaagaacc aaactaacca taaaaattgg attgatcaag agctttttaa tcacctctaa
      301 tggcatggaa gcacattgaa tgtttagaaa atgaatgaac aatcgttgat atcttggata
      361 aattgataat tgagaaaaaa ttaaaccgca acacaatatt gttgcggcta aaaaggatag
      421 attaaaaact aatattctta ttattttcat catttcatcc taattatcaa aaaataattg
      481 agcaaaaaat tcccaacaat tttcaaaaaa gttcttcttt acaccagttg acacaacttt
      541 tttaatttct tctttcggct ctgatttctc tgatttaggg tataaccaaa catccgtcaa
      601 aaattcacct ttttcatttg aaacaggtgt caataaaaca atatcttcac tgtttgaaat
      661 gttttcaggg cttgattcat taacgatata gtatgcttgt tttttgtttg caggtacttc
      721 aatggaaaat tcaatcacgc cttcctgatc atcaatcgtt ttggtttctc cttcagccac
      781 ttttttcaac tggtcgtatg aaagtttttt tgctcgatct ttcaattttg actcaattat
      841 ttctacagat tgatttttct cattatcatc ttgtgacatt tcttctaaga tattcgtgat
      901 atcgtagaca ataaacttcg cgccattgat accttcttgc tgggctactt gactgccttg
      961 attgttgatt tgattcgaag aaataatttt ttgaatatga atggcttgct tgatcacttg
     1021 cgtatcttct acgtcttgcg gcttttctga agtctctgaa gaactttctg acgttggatt
     1081 cgttgattca gtaaagctta tatttgaatc tgccgtttct tctgtagtat tctccgaact
     1141 gtccgtttcg cttggtgcag taattgaatt atctattgtt tgagtggtac tactatcact
     1201 gctgtcagcg aaagacagtt gcgaataagc tacagtagaa aagagagctg ctacacaaac
     1261 aaacagccat tttctcttat tcattccgat ctcctcctta tttttttata aataaaaata
     1321 ataggaacgc aaaaaaaagc gaatagcagt gctaatcgcc aatccaatga aattgatttc
     1381 ctattttttg cttttttctg tagttttaaa tcttctttac tgaaggaaac tcgttcacca
     1441 tacactaaaa agcggtgtga gttgacacct tttggtgtgc atgtcattaa cgtgatccta
     1501 tcttggtctt tttcaggcat aagataatct aaataatttt tttgtaattc ttctgcttta
     1561 acttcttcaa ttcgataaat ttgataggca tgttgttctt catttacctt aataaagaat
     1621 ttttgccctt tttttactga aggcaaatca tcgaaaagat tatctttata aagtccactg
     1681 tgccccgcga ttaaaggatt tttaccgttt cctcctgtaa tatcgccagt tccttccgta
     1741 atccctacac cattctctaa aattttgtcg gaagttccgt cataaattgg tagttttaaa
     1801 gaaatacttg gaattgtaag aacaccaaca ggatcgccta actctatttt cgcaacatcc
     1861 aaagaagaat ctgttttttc aagattttca atggctaaaa ttctttgctg ttgttgaaat
     1921 ttcgctacat ttgtttccac aactggaatt aaatagactg ctactgccga cagcaaccaa
     1981 aaaaatatca tcagtttgtg catgactctt ctttttgggg gcttacgaat aatcattagc
     2041 tgatccttct aggctttctt cttaaagtaa acaactgcag aaagtccccc tgctcctagc
     2101 aacaagcaag agatcaatac gtaagattgg aaaccgttgc taccagtcat tggtacggaa
     2161 gctttttgat tatttttgat cgtgattcgg ttttcttctt tgtatgaatc tttgtctact
     2221 ttgaaagaga tctcttttgt taatttttga tagttttcag gagcttttgt ttcttccagt
     2281 gagtaatctc cttcagttaa accagtgatt tcaaaatgac cctctttatc tgattttagg
     2341 attgctgcat ttgctacatc gtctacccaa actgcttctt tgacaccatc tttatctgta
     2401 tcagtaagtc caccgtagta cttaccttct gcattcttag ctgcaaattc tgcgccttca
     2461 agcggtgttg ctttgatacc tgttccaaat aggccgctat cgacttttaa gaattttgcg
     2521 ccataagtca caacgtcagc tttcgttttt tcgtggtgtt cttcctgatt aaatggatta
     2581 ttgttataaa cgaaatccac gctatttgtt aatggatttg cgatttcagc actatcattc
     2641 acaaacatgt tataagtcat ttttagatct tgaccatgta aattcgcaac tgtatcactt
     2701 acttgtccat tatttacgat aaaatcgatc acaaaaccac tatcagaatt ttcgatttta
     2761 tagtcagtat tttcttttaa tacgactgaa ccatcattat ttgttacttt aatatctgct
     2821 ttttcagcaa cataagaaag tcctttacct gctgtatctc cccatttaaa tacactgtaa
     2881 ggagaagttt cgcttttcac accgtttact gtgataccgt ctgcgccacc tgcaatgtct
     2941 gttggaatat gaatcgttcc ttgataattt actgcgtcac caatttggaa agaatgttct
     3001 tgtcctgttc catttgttac tgttttatcg atcactggtg ctttatagtt tacgtatgaa
     3061 gcttttaagt cacttgtcac gccatcattc gtaatttcaa aggttaattt attgtgcgca
     3121 tcgttgcgag cgtctgcgcc taataaatat tgatctgctg ttggttcttg atctgatccc
     3181 actacaactt ccgaaggaac ttccacaaaa tagtattttc ctaacgtcaa atcagtcgca
     3241 gtcagctttc cttgatcatc cgatacaaga tcacccaatt ttgttccttt tccaggttcg
     3301 cccttgtata actcaaattt tgcccctttt aaaagggttt gtttgctttg gtctgcgcca
     3361 tcgtcaccaa atttcgttaa ttcaaaactc aacttagaaa caatgttctt aggatacaaa
     3421 tgaatatttt ttaaaaatcc tgatcctgtt ttatctgtca ttggcgcaat cacaaccatt
     3481 ggttttgctt tttgtgtgac taatccagca gcggatttac tttcataaac cacatataca
     3541 ttccctttgg aattgacata ggctggttga tcagtaaaag tgatctcacc attttcatct
     3601 acagctgaag taattttgtt ggttgcattc ttgacgtact cactgttaga tcccttgtct
     3661 tctacatctt gtacaatctc gtccactttc gcgttggtta gatcagaatc ttctctcggt
     3721 aatacttgat ccgtaatatt tcctaacgta aactcaacat caccataatc tgccttgttg
     3781 tatttcgtta cacctgtagg atattgatcg tgcgtataac catcgttttt gatcccgtca
     3841 atatcaacat tcaattgcgt tccttgatcg tacattaatt tatgaaccgt gatattgacc
     3901 ttctctggtg gtgttttttg cgtgacatct tctgcatgta cttgataccc ctgaaagcag
     3961 cctgcaatga ttcctaacat tccaacaagc attccagctt tttttacata ccgatttatt
     4021 gccatttttt actctcctca tttcatattt caacgactcg cgttgattaa aaccttgtta
     4081 ttgtagctct ccctgtacca cgtatctttc ttttccttgt gacgtataaa cacaagtaaa
     4141 caacgttaat aagggtttct ctcctttctt tggcaaagac aagaaagagt tctcttcagg
     4201 aacgccatcg attaatttaa agtacccagg tacgataaat ttacgcgtta ccttgtaacg
     4261 atagacgcca tcgttactaa ctaagtccat cacctcccct aatgacaact tttgaatatc
     4321 agaaaataaa acacctggca tttccatgtt atgacctgct aggacatagt ttcctttgcc
     4381 catttcggca tcttcataaa aataagtagc cgctccaaga ctcaaagtgt attgatttgc
     4441 ccctttaaaa attggcaatg ctaaaccaag tgcaggaatc tgaatttgtg caaatacttg
     4501 tttattgacg cccatttcga ttgcttcttt ttgtatggac aacatttgcc ccaaagacat
     4561 atcattattg acgttcgttg catcctcctt ataactcttt gcatgtgcat tgatttgatc
     4621 tacgggcatc tttttttcta tattttgctt aaacacctct ccttcatgtt tttggtagct
     4681 aacatagcca aacacactgc aaaagacgat taaagtgact cctaaaaaac caaataacca
     4741 ttttttcatc catctgcctc ctttcagaca aaacaaaaaa actttttctc atttccatag
     4801 aaaaagtttt aaaaatgaat tctataagtt cgtctcaact gttcaaagtt tttcattgaa
     4861 cctaacaaac aagctttctc ttttcctgtt aactgcatat cattgatata ttgcgcgaca
     4921 gataatcttt taaggtgttt ttgcttcatc gtttttagta acttttcttc catttctttc
     4981 gacacttttt ttctgttttc gatttcttca taattcatga gttttcctac cttccttgtt
     5041 ttttgcaaaa ataaaaaagc ctatgatcac atacgctcta cacttcagtc ttgggttatc
     5101 tttttctttc gtttaattac tggccgtgct ggtttcaaat ccttgattcc attatgggtt
     5161 ctccaataat cgaattcttt tcgcaatctt tctttttcca tatctgttcc tttagataag
     5221 gctttgaata cttctttagg tgaataacca cgagctaaca gctgtacata ccttcctttt
     5281 gtcaaagtat ccatatccat cgcctctaaa actggtcgcg ccatcta
//
LOCUS       DACDIP010000110         5078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDIP010000110 DACDIP010000000
VERSION     DACDIP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.111"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            184..552
                     /locus_tag="IV257_002662"
                     /pseudo
     CDS             184..552
                     /locus_tag="IV257_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            652..852
                     /locus_tag="IV257_002663"
     CDS             652..852
                     /locus_tag="IV257_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815727.1"
                     /translation="MLKRIAWDKDNKTVIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            986..2212
                     /locus_tag="IV257_002664"
     CDS             986..2212
                     /locus_tag="IV257_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815728.1"
                     /translation="MIFYMFININEVLKMTTITMTKNIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYRNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRLKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            3137..4461
                     /locus_tag="IV257_002665"
                     /pseudo
     CDS             3137..4461
                     /locus_tag="IV257_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            4454..4801
                     /locus_tag="IV257_002666"
     CDS             4454..4801
                     /locus_tag="IV257_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815729.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
BASE COUNT         1967 a          619 c          956 g         1536 t
ORIGIN      
        1 ttacttgaat catggtctat attatcgtgt tatgagtgaa atttctaata aagataagat
       61 attttatttg gataatcatg tgaatatgaa cttaacaatt aaacgtattt acgatctttt
      121 atcacttata taaagagaga agtgataaat aggacacaaa atttggaaag taggtaagtc
      181 actatgagag aactaacttt atcttctgag gaattagaaa agaaaactgc tttattaaac
      241 gaaatattta gtgctgattg tataatccaa agtttaaaag aaagttcatt ttcaaaacta
      301 acagatcaga aaaaaatttc ttatatgcta agtttagata atctaggaac taaatatgat
      361 gaatcggcaa gtagcgataa gcttttgatt gaattagtat caagtcttag tagttttgga
      421 aaaatgaaaa gatttaatgt gatattagaa atttttgctt ggataatccc ttcatttatt
      481 tggttttttt atattttcat agttgaatct gagttaatag ccaaggtctc tcttgaactg
      541 ttaattccga tatatgcatt ttatttaata ggttcagcaa tttactttaa taacttgtat
      601 acacgtattc aaataaaaaa gcagttatta catgaaggat taagtggtaa tatgttaaaa
      661 agaattgcat gggataaaga taataaaaca gttattgagc ttaaagaata taaatacaat
      721 tatttctcga gatgtataga tagaaagtta ctgtttaaaa aagaaggatt agaatttagt
      781 tttgatgaag atgaatttga aaaattagtg atggactggc gtaagaattt actggatacg
      841 gtacaattct aaaagcaatt gaggtcgaaa ttgcgatact tgaatcatgg tctatattat
      901 cgtgttatga gtgaaatttc taataaagat aagatatttc atttggacag tcatgtgaat
      961 atgagcttaa caattaaacg tatttatgat cttttatatg tttataaaca ttaatgaggt
     1021 gctaaaaatg acaacaataa ctatgacaaa aaatatagaa gtatatgttg atggatcaca
     1081 agaagaaagg gattcctttt ttgatttttt ttggaaaaat agtagagaat atgtaaaagc
     1141 gttgaatctg gtatataaac agctaatgtt tgaagagttg ataaaagatg caattaaaga
     1201 aatggatcaa ggttatgctg agagagaaga aagtattcaa aatcggattg aaaagttagc
     1261 atatactgat ccaatagatc aaaaaaaact tgataaagca aaagctgaat tatctaaatt
     1321 gagaaatgaa aaaaataaag aagcgagaga aatattaacg caagcgatag ggttgaagag
     1381 tcaaacgaga acacgagatg tagcaaaaaa attagacttt gactttggag attttgttga
     1441 ttcagcaaat atgaaagcta gtaaggattt tggcaatgat tttattggaa ttgttactgg
     1501 tcaaagaaca cctagaatct atagaaataa aagtaaatac ggattgttac cgttcagagg
     1561 tcgggatatt aagattcaca aagctgacaa gttttatatc tctttaccta aaggatttag
     1621 acttaaagta aatttaggta gcaagccaag aaaagcaaga gatgttgctt cagttttaca
     1681 acacattatg gatggcgatt acaaattaaa tcaaagtaat cttatttttg atggaagaaa
     1741 attatttttt aatctaacaa tttcttttga aaaaagctat aatgaaatgg aattcaagaa
     1801 aggtaatgaa ttgatcgtaa actttgcaga tttgtgtagc ggtgaatgtt taattaatga
     1861 taaatctgta atgtcttttg gtgatagcca atatataaga aacttcaaaa gtaaaatttt
     1921 agcaatgaaa tcaaaagaac aaagccgagg agtcttttct aagggcggtc atggtcgaaa
     1981 aagaaaatta gatcaagatc gatgggaagc aataaagaaa agagaaagta actttgttaa
     2041 aacttataat aatcaatgtt ctgcaaaaat cgttaaaact gctattcgca ataaagctca
     2101 aattattcgt atcattgaac cagataaggc ttatggcgaa tgggcttact atcaattgaa
     2161 agaacaacta gtaaacaaag gaaaaagata tgggatagaa gtcagaagtt agtaatttaa
     2221 acaatcgttg atttggcaac gaaatagtga gggaaaatcc actcactgaa gggtaaactc
     2281 cgtcaagttg tgtgaccaga cggaaggacg aatgttgttg aggttttaga actatataaa
     2341 acagcatgtt tctcaaatta tgagatgtta agaaaaatga ttcgagaggt tttagaacta
     2401 tataaaacag catgtttctc aaacaaagtg cagaacgaaa atcaattcat cacagtttta
     2461 gaactatata aaacagcatg gttttaaaac aagaaataag tcagttttcc ttttgcttta
     2521 gtaaggaaaa atagagtatt ctcaataaaa cttaaaataa gaaagaataa gcaaaccttt
     2581 gttttaatag ctaatcgaag taaaacccaa agccatatga actcctgaat cagcgaaagt
     2641 ttatgatgat tgggcttact aataatttag gaattagatg gtaaatctaa attaaacaat
     2701 cgttgatttg gcaacgaaat agtgagggaa aatccactca ctgaagggta aactccgtca
     2761 agttgtgtga ccagacggaa ggacgaatgt tgttgaggtt tagaaccaca taaaacagca
     2821 tgtttctcaa attgccttag tggttgaatt gagatctatc acgttttaga accatataaa
     2881 acaacatagt tctcaaattg tttaaaatga ttgtcaagtt aaaaaaatct gggatcatat
     2941 aaaatagtat agttctcaaa tatgattaga aaccctatta aagagcaata ttaaaccaac
     3001 ttacttaaat cgtttaatta agaacagtaa tctcttttag tcagactttt agtctgactt
     3061 ttttgtattt ccaaaattta ttctttacaa aagcgaacgt acgtttgtat aattgcttgt
     3121 gaatggggga ataaaaatgt ttgattacag tagagaaccg gtgaatgact attttttaat
     3181 cgacatgaag tctttctatg ctagcgtgga atgtattgaa cgaaatttag acccaatgac
     3241 tacaccgtta attgtcatga gtagatcgga taacactggt tcaggtctga ctctagcttc
     3301 ttcacctact gcaaagaaaa aatatcatat cactaatgtt agtagagcta atgatttacc
     3361 ttatccatta ccaaaagaac tttatattgt tccacctcga atgaacttat atataaaaaa
     3421 aatatggaag ttaatcagat ttttagaaag tatgtcgcag atgaagattt attgatttat
     3481 tcgattgatg aatcaatttt aaaagtgaca aaatccttaa atttatttac tagttcagaa
     3541 acaagaagcg agaggagaag agaattagct aaaataatca aagatgagat tgagaaagaa
     3601 acaggactaa tagcaacagt cggaatagga gacaacccat tattagctaa actcgcactg
     3661 gacaatgaag cgaagagtag taaagaattt atagctgagt ggacttatga gactgtacct
     3721 gaaaaaatat ggaaaataaa cgagatgact gacttttggg gcattggatc acgcatgaaa
     3781 aaaagattga atcatatggg aattatgagt atagaagatt tagcaaattg gaatccctac
     3841 acaattaaaa gtcagttagg tgtaatcgga cttcagttat atttccatgc caatggaatt
     3901 gatcgtacgg atattgctat tccaccagaa ccaaccaaag aaaagtctta tgggaattca
     3961 caagttctac caaaagatta tacgaggaga gaagaagtag aactagtagt caaggagatg
     4021 gcagagcaag tagccattcg aattagacaa cataattgca aaactggttg tgtacgctta
     4081 gcaattggta cgtcaatttt ggaagaaaaa tcaggtttct cccaccagat gaaaatacca
     4141 attacagata atacgaaaga attacaaaat tattgtcttt ttttattcaa taagttttat
     4201 gaggggcagg aagtaagaca tataggagta acttatagca gattagtata cacagattcc
     4261 ctacaattag atttatttag tgatccaaga aaaaagattg ctgaagaaaa tttggatagg
     4321 attattgata agattagaca aaaatatgga tttacagcta ttatacatgc ttcatctatg
     4381 ttagaaggtg caagaagtat tacgagaagc actttaatag gaggacacgc aggaggatta
     4441 ggaggtttgc ataatgattg aatataacga tagaggacgt ttaaaatggt cgggatttta
     4501 tttgtctgat catactgaaa gaatcaatgc agacaatgta caacgtgcta atcataattt
     4561 ggcaaaagaa caaatgacga ctgaagagat tgcgaaaata ctgagaactg ctgtcataaa
     4621 aaatagaccc gtctgcattc aaaaagagga acgaaacgct gaaggttact atccagatga
     4681 tgtagtagga aaaattattg gatatgacga gcttggtgta tttgtggaag aagaaaaagt
     4741 tcactatgat gaaattagga atgtttcttt cgcggaatta ttaaaatgga gtcagatatg
     4801 aacattatta aaaaatacga attcggttat attacgtatg aacagctaat agaggaatta
     4861 tggggatatg gtcaacaatt aattaatgaa gtggggattg attgcttttg tttctatatt
     4921 gaatccggag caggatacca taagaacaga tactatatat ttccatatcc accaaaatag
     4981 tctcttagag atttttaaat tttctccaaa tactttcaat tattgtgaaa tatttccctt
     5041 taacagaatt aggcagaagt cgcccatcct ctataggt
//
LOCUS       DACDIP010000111         5059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDIP010000111 DACDIP010000000
VERSION     DACDIP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.112"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(627..1631)
                     /locus_tag="IV257_002667"
     CDS             complement(627..1631)
                     /locus_tag="IV257_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8815730.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(1702..1998)
                     /locus_tag="IV257_002668"
     CDS             complement(1702..1998)
                     /locus_tag="IV257_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815731.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1991..2284)
                     /locus_tag="IV257_002669"
     CDS             complement(1991..2284)
                     /locus_tag="IV257_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815732.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(2430..2981)
                     /locus_tag="IV257_002670"
     CDS             complement(2430..2981)
                     /locus_tag="IV257_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP8815733.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(3155..3454)
                     /locus_tag="IV257_002671"
     CDS             complement(3155..3454)
                     /locus_tag="IV257_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8815734.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(3481..3789)
                     /locus_tag="IV257_002672"
     CDS             complement(3481..3789)
                     /locus_tag="IV257_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8815735.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3906..4865)
                     /locus_tag="IV257_002673"
     CDS             complement(3906..4865)
                     /locus_tag="IV257_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8815736.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
BASE COUNT         1483 a          964 c          733 g         1879 t
ORIGIN      
        1 aactcataat tcccatcttc tgatttattt ttgggaacaa ctatcaaact tttataaagt
       61 ttatccacat caaaatttat cttttttgca taatttgtag ccattaatac atctccttat
      121 tcataataaa tttcatatga cgctcctctc aaaatttacc ggtagactag aaaaaagaat
      181 aaaagatacg tcaattcaca ctttattctt aaaaaaaaac tggtctctct accggtaata
      241 tcgattgagt ccgcctttat cgactttatc ctttttttat attaaatttt ttcaccggta
      301 gacagggaga gaaagaataa actaaaaagc ccttaattct taatgttttc acctccttac
      361 cgttcctacc gctaaaattt aatataacta aattctataa cattagaaga aattttaaat
      421 acctttgata aaaaatattg atgttttttc tctcattgtt tacaaactag atagcttatg
      481 tattcttatt aaaaataata gatatttcga acatcatcac tctcatctac ttaatttcgc
      541 atttttactt tataaaaaaa ctgattctaa gaaaattaaa taatttttct caaaatcagt
      601 ttttctttga taaataatat tttcaattat ttaatcattt gaaaattgtt agtcattcca
      661 atacgttgag aaattcttcc atctgtataa acacggaatt gttttccacc agccattgtt
      721 gtatttccat tatcatccgt tacaatatta attgaatagt agcttccatt ttcatcttgc
      781 attagatctt tcatatattc atttgtgcta attaaattag tatttgatgc atttttatca
      841 ggagaatcat acaaccataa agctttatta tttagtacag tttctttagc agcttcccaa
      901 cttgaaattg ctgagctact tgtagattgt gtcgttgtag ttgttgcttg ttgagtagat
      961 tcttctacta ctggagtttg aggagtttgt ccattcacaa tatcaatgaa accttgtttt
     1021 aactgttggt tctgagtttc attgaagtac atgtagtttt tatcattgcc ttgattttgt
     1081 tgcgttaaat aaactttagg ttgtccattt tgaaagccaa agaaatatac atgctgccct
     1141 aaatattctc cagtttctac atcagaataa actgctacta aattgaaatc tgcatgacca
     1201 gttccatctt ctgaccattg tacggtaact ggtgcttcat tgattgccat tttccaattt
     1261 ccatcaataa ctgcacttgg taaaggcgta ccatacagac ttacattgtt ctgaggattg
     1321 tagcttttat attcttgacc taatgtcttt ccccattgtg ggacaaaaat tgctagttga
     1381 gtagatttct cactatccca caaagtattt ttaacctctt cagaagaaga ttcaatgatt
     1441 gaactttcac ttgtacttgc ttccacagat tgtttcattt ctgttgtagt ttgttcagtt
     1501 gattgcgttt tacttttttt agcctcaatt gttgtatcag tttctacagt ctctttagtc
     1561 ctcgtattct cagatgtaca accagtgaat aaaatcccca ttccaattag taataacaac
     1621 cattttttca ttttatttcc ccctttcaat ttctccctcg cgtacttatt ttatcagtca
     1681 attaatctca atggtgttca tttagttaat cctttcccaa gaatgtcgat agccagcatg
     1741 acaacgatct cttccttgag ggctttcaga tggattaaaa ctagtagaaa tttcccttcc
     1801 acatcctcta cagcgataag ttctcattat ttttgggcta ttagtattat ttttcttagt
     1861 tagagctatg agtccaacta tgattacccc aactgtctta attacttttc ctttatctat
     1921 attcggatgc gcaagcatat atttatataa ccaatatcca ccgtaaatta aaccacctaa
     1981 tacaatcagc ttagccatgt actcgtctcc tttctttttg tttaattact ttttgaatcc
     2041 atcttgcaga gaaaccataa acttctgtca attgacggat atctacagtt ttttcctgtt
     2101 ctatcatgtt ttcgattctt tttctaacta actcacgatc gtacattttc attggaaaag
     2161 aaatctgttg ccctcgaaaa gcaagataaa attgatagag caattctgaa tccagttcca
     2221 tttcctcaaa aaagtcattc atacttttat aagagttact caaacaagtt tcatcaatct
     2281 ccatctacac accccctgtt attttttaca taatgaaaat agcatattac tgggtacatt
     2341 gataattcat ataacatgta tcatcacatg tatttttaaa gtcaaaaagc atgtaaaact
     2401 acttataagt aacataagaa aataaaatgt tatttttctt gaaataataa ttgactatct
     2461 gccaattgcc attcattacg aacatgaata cttagtgggt gggataactt accttctgca
     2521 tttatcaata ctttggtcat ctttttttgt ttcgcactca attgatcgta aatggtcttc
     2581 gcttgttcat aagccacttg actcgtcgct gtaatcgaac cacaagcaaa aataatggtg
     2641 ttgattttct tttctgtgaa aacagttgtt aatatttcca aagattgttc atcatttcca
     2701 cgaggaaata cccgaggact tttcgctttt gtatacgaaa aaagattcac agcaataaaa
     2761 ccagtaaatc ctaattgtcg gatattcttt tcgattaata acatactcaa atcattcgta
     2821 aatggttctg tatctgttgg acgaatagta atgactaccg ctaatttatc ttccgcttct
     2881 tttcctttga tcgcccattg tcgtttgaaa acatagcgat gggtatgatg aggatctcgc
     2941 caaacttctg tttgtgtggt cactaattct ttttcaatca tctctttttc tcctatgcta
     3001 atttttgttc aagcaattca atcaccattg ccgttataag tgtcaatact agttgaaaat
     3061 atttcatatt ctttctcctt ttttcttcag aatttgatga atcgctgtaa ggatatcttc
     3121 taaacatttc gcaatcattc tcaaataatc gatcttattc attcgttcct ccaatctgac
     3181 tccaaaccaa tcgtccttct accagtaacc cacgaacaaa ttctcgctgg ctggtaatat
     3241 ctgtaatgaa ataaactaca tagtcttttc catcttctgg tgcgcaataa atataaagat
     3301 gtgtcatcac taaacgtggt agagtaacat cttcaggaag catacccatt tcaatttcct
     3361 ctttttccat cgtaacggat tcatagcccc actcttcagg agtagtgact agctgtgcac
     3421 acttttccag tacttcgata gccgtttcta acaagtcctt tcgttccttt cttttagata
     3481 tcagtattct tctggtaata acatcgtttg atactcaccc gaatcaatga cccatatttt
     3541 ttgagacact gatttcaaac gaagcttagg tgaaatcaga tactcttttc ttgcttctgg
     3601 ttcttcttga cgatgaacca acttttgatt tcctttttcc gtagaaaatt caaagatttg
     3661 aagatagtct aaatgaaatt gcttttgaac gttttgatcg attagattcc aacataaagc
     3721 ttgtaattcc accggcaaac tgtccattac tccctttgtg acataacgtt gtcctttatt
     3781 ttgaaacatt tcttttccct cctttgccat aaaaatagcc caatcttcta ttaaagaaga
     3841 ttgggcttca tggatataat atatatttga tattcaattt gattcatttt atactatctg
     3901 atttgtcaaa ttaagctaga caatatgtct ttatctatgc agctcaaaaa tacttccaat
     3961 ggtgttcgat aatttaatga ttttcttgga atatggtttc ttttcgaggc aacggattga
     4021 ataaaggttt catccgtgtc cctaaagtcc attgttttag gtaagccatc tcgtctaagc
     4081 aagccgtttg agtgctcatt tagccctctt tgcgaaggtg ttcctggatc tgcgaaaaag
     4141 atggcaatat cgttcgagtt acttatagat cgccagttgg aaaattcttt tccacaatca
     4201 aatgtcatgg atttaaacaa gtgtttaggc atggatttga accaatcatt taacctgttt
     4261 tcgatatctt cggctcgtct gcctgtcggt ttcaaagtaa taataacctt ggataaacac
     4321 tcaaccaatg taataacagc gctcttgtgt ttaactccca caatggtgtc gacttcgaga
     4381 tgaccaaatt ctttagtcag gtgtggatag agactgttcc gttcatgaat cgttctgcga
     4441 aaggcctgtt ttccacgctt ttcttgatga ttattcggtt tacgtttccc cttcataggt
     4501 aacgactgtg tgtcaaaaag gcctttttta aacatccgat agagtgtacg aacagaacaa
     4561 tcaattggaa actcgccacg gccgataatc acatcgggtg accatccttg agctaccttt
     4621 tcttgaacat atgttgattg ttcatcagat aaaacggttg aacgtcgtcc acaacgagat
     4681 ttattttgtt tgtattgacg gtaataatct agcacttttt ttccttgttt gagaaacgaa
     4741 taaacattat aaatggtttg tctagagcga tttaaacttt gtgcgacaag agcgacaggt
     4801 ttctcgatat tgaaataaga ctctatgatt actagttcgt ctgttgtaag atgggtgtaa
     4861 gtcatttgta ctcactcctt attaattctt tggtcggaac tattttgagt gtagcacaaa
     4921 tgatcttttt agttgtctag cttaatttta caatcgacgt tttaataaat tgtgatataa
     4981 tgaagtataa ctttttaggg agtgtactat gaattttcaa gataatagac cattatattt
     5041 acaagtagaa gcttctata
//
LOCUS       DACDIP010000112         4933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDIP010000112 DACDIP010000000
VERSION     DACDIP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.114"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            206..1480
                     /locus_tag="IV257_002674"
     CDS             206..1480
                     /locus_tag="IV257_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8815737.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1534..2961
                     /locus_tag="IV257_002675"
     CDS             1534..2961
                     /locus_tag="IV257_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8815738.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2974..3846
                     /locus_tag="IV257_002676"
     CDS             2974..3846
                     /locus_tag="IV257_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8815739.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3941..>4933
                     /locus_tag="IV257_002677"
     CDS             3941..>4933
                     /locus_tag="IV257_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8815740.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
BASE COUNT         1694 a          682 c          974 g         1583 t
ORIGIN      
        1 tataaaaaat agtgccatta aaaaagaaaa gcgacacacc aataaaagtc atatcctgtg
       61 acattatatg gcattatcca aatagaataa ccggttgaca tgtaagcgga tttcttttat
      121 aattttagga gtacaagtcc ttttctaaaa atgttaaccg gttatcatga aaggggatac
      181 aaatatatcg ttggaggata aaaatatgtc aaaagttaac gtatttaaaa atcaatctta
      241 tttacaaagt tcagctacat tattactatt ttttgcttct tggggtgttt ggtggtcatt
      301 ttttcaactt tggctaacat ctgaatcaaa tggtttaggg ttatctggca gtgctgtagg
      361 aacagtattc tcggcaaatt cgttagttac cttaattttg atgtttattt atggaacatt
      421 acaagataaa ttgtatatta aacgaaattt attaattttt gcttctgtat tagcgacact
      481 tgttggacca ttttttatat ggatatatgg gccattgcta gataacaatt ttaatttagg
      541 cattattatg ggagcgctat ttttgtcagc tggatattta gcttctgtag gagtttttga
      601 agctgtgtca gaaaggttta gtcgtttatt tggctttgaa tatggacaag caagggcgtg
      661 gggatcattt ggttatgcct tggtagcgct tttggcagga tttttatttg taaaaaatcc
      721 tcatttaaac ttttgggcgg gatctttctt tggttcttta ctattgttaa atttattatt
      781 ttggaaccct aaagttgaac gggaagcaaa tcaaaatttt aatcaagaac aagctgaatc
      841 aaatagtatt ccttctttaa aagaaatgtt tgatctaatg aaactgcctc aattatggac
      901 gataatcatc tttattgttt ttacatggac attttatacg gtattcgatc aacaaatgtt
      961 tccgggattt tatactggtt tgttttcaac atcagctaat ggtgaaaaaa tatatgggac
     1021 attgaatgct attcaagtat tttgtgaagc gttaatgatg ggaattgttc caatcattat
     1081 gagaaaatta ggggttcgaa atactttgtt attaggtgta accattatgt gtgtacgaat
     1141 tggattgtgc gggtttgcct cgacaccatt atctgtttca tgcataaaaa tgttgcatgc
     1201 tttagaagta ccattattta cattaccaat gtttcgctat tttacacttc attttgatac
     1261 aaagctatca gcaaccctct atatgatagg atttcagata gctgctcaaa ttgggcaagt
     1321 gattttatca acaccattgg gaatattaag agacaacgtt ggctatcaac caacatttaa
     1381 aattatttct cttattgtat tactagcagg catatatgca ttctttattc ttaaacaaga
     1441 tgatagagat gttcaagggg atccatttat tcgaggataa tggaaaccaa cagaataaaa
     1501 aacaatttta tctagaaagt gagcgaatat attatgacaa aaagagctat agaattaaca
     1561 aatacacgtt atcgtttggg atatcatgtg tctgcaccat caggatggat taatgatcca
     1621 aatggatttt gctattttga tgggtattat catgtgtttt atcaacatca tccatatagt
     1681 gctgaatggg gaccaatgca ctgggcacat gctagaagta aggatttagt tcattgggaa
     1741 tctttaccac ttgctttgac tcctggagat caagaagatg aaggtggttg tttttcagga
     1801 agcgctattg aaaaaaatgg tgtgttgtat ctgttctata caggacatca ctattatgga
     1861 gacaatgatc cagatcattt ttggcaaaat caaaacatgg cgtatagtac ggatggcatt
     1921 cactttacta aatatgaaaa aaatcctgta atagcaaaag caccagaaga taacacacat
     1981 catttcagag atccaaaagt atggaaacac gatgaaaaat attacatggt tttgggtagt
     2041 caagaaaaag atggattagg tcgtgctatt gtttatcagt cgttagattt attaaattgg
     2101 gattacattg gaccaatttc taaagcgaag gaagtactta cagaaggatt tatgtgggaa
     2161 tgcccagact tttttgaatt aaatggaaaa tctattcttt tattatcccc tcagggaatt
     2221 gaagcaaatg gaaaagatta tttaaatcta tatgaaacag gctactttat cggtgattat
     2281 gattatgaga cagcaaaatt cattagagga aatttttatg aattagataa gggacacgat
     2341 ttctatgcaa cacaaacaac tttatctcca gatggtagac gtattgttat ggcctggatg
     2401 gatatgtggg aaagccctat gcctgaaaaa gtagacggat gggcaggagc attaacgctt
     2461 cctcgagaat tagagttaat tggagatcgt ttgtatatga aacctgtgag agaattagaa
     2521 caacttcgag ttggcagcgg cgttgaaacg acagttaatt tggcaggaga aataataatc
     2581 gctcaagatg cttcattcaa agaagctcta attgatgtac tattgactaa atcaaaaaat
     2641 caagaagtca aattttcatt aaaaacaaaa gatgaagagt taatcaatat tttttattct
     2701 aaggataaaa atgaatgtac cttaactcgt aacggcaaaa atgatccaag gtatggaacc
     2761 attcagatgt gtgatagatt atcacttcga gtgtttattg ataaaagttc ggcagagatt
     2821 tttattaatg atggcgagct tgtttttaca gagcgttatt atacagaaaa tcaaccaagt
     2881 atgcatttga gtgttagtga agaagctttt gtctcgagta caatttatca tctaaaaaaa
     2941 gatgcaattt cttacgagta aggaggtttt atgatgttaa ttggtagcat agaagcaggt
     3001 gggactaaat ttgtatgtgc cgtgggagac gaggattacc gagtacaaga ccaaataatt
     3061 tgtccaacga ccacccccag ggaaacattg aaaaaaactg ttgactattt taaacagttt
     3121 gatataaatg cattaggtat tgcatcattt ggtccaatag aaattcgaaa aaattcacca
     3181 aaatcaggat atattacgtc tacaccaaag gtgggatggc gaaatgttaa tgtgttaggt
     3241 tatttacaag atgagctgaa tgttccaatg tcttggacaa cggatgtgaa cggatcagct
     3301 tatggagagt acatcatgtc cactttatcg aatgaaaaaa taaattcatt agtttattac
     3361 accgttggta ctggcgttgg tggtggagct gttgttgacg gaaaactatt aggatcgttt
     3421 ggtcattcag aaatgggcca tacgtttgta aaacgccatc ctgatgatat tgattttaaa
     3481 ggaatttgtc cttatcatgg tgattgttta gaaggcttag ttgcgggacc aacgtttgaa
     3541 gcacgttttg gaaaaaaggg tgaagacgtt tctttaacgg atcctatttg ggatgtaatg
     3601 gcctattatc tagcacaagc tgtgattcaa acaacattga ttatacgacc ggataaaata
     3661 gtatttggtg gaagcgtagt aaatgaaaaa ttgttagaaa aaattcgtgt taatgttaat
     3721 aaattattgg atgattacgt ggaaatacca ccgttaagta aatatattac gcgtccttta
     3781 attaaaaata atggatcggc aacactagga aactttgctt tgggcttgaa tcaattacaa
     3841 gaataaaaca tagaaaaaat atcgattatc aggtatagta gttctataga gttctttttt
     3901 tcgatagtta attattgcat tatttaggag agacaagaga atgcgcccaa aattagaaga
     3961 tgttgccaaa agagcaaatg tatcaaaaac aactgtttca cgtgtattaa acaatcgtgg
     4021 ctatttaagt caaaaaacga ttgataatgt ttataaggct atagaagaac tcaattatca
     4081 accgaacgtt gtagcgcgtc aattatttca aaagaaaact aacattgtcg ggttactatt
     4141 tcctacagta gccaatcctt tttttagtga actcgttgag gcattggaaa aaaagttata
     4201 tgaaattggt tataaggtat taataggcaa ttcgatgaat aataaagaaa aggaaacaaa
     4261 ttatttaaat cagttattgt ctgatcaagt agatggttta attgttggta cacataacca
     4321 aggaattcag gaatataaat atcagaattt accgattgtt gcaattgatc gagtgatgaa
     4381 tgaagatatt ccagttgttg aatccgataa ctacaatggt ggaaaacttg caaccaagtt
     4441 attaattgca caaggtgcaa aaaatattat tcatacaaac ggtccaattg acttacagac
     4501 tccggctaat cgtcggagac tagcctatga agatactatg aaagcatatc agttaattcc
     4561 acgaactgtg acattagatt tcaatatttc ttatgtaaag aaaaaacaga tttttttcca
     4621 aatgtttgag gattatccta aaatagatgg tatttttgct tcaaatgaca tcgatgcggc
     4681 cctaatatta caagtagcaa aagaaaaggg gttaaatgta ccagctgatt tattagtagt
     4741 cggatatgat ggtactttaa tgactcggag tatattacca gatctgacaa cagtaataca
     4801 accaattaat gatatagcag acacagctgt tgctattttg atgaaacgaa taaataaaga
     4861 agaaacaaaa aaggaatata ttttacctgt cacattatgg atggggaaga catcagtaaa
     4921 gaaaaagaac aat
//
LOCUS       DACDIP010000113         4892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDIP010000113 DACDIP010000000
VERSION     DACDIP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.115"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IV257_002678"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IV257_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8815741.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="IV257_002679"
     CDS             complement(2064..3062)
                     /locus_tag="IV257_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8815742.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="IV257_002680"
     CDS             complement(3288..3941)
                     /locus_tag="IV257_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8815743.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IV257_002681"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IV257_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8815744.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1446 a         1019 c          753 g         1674 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa at
//
LOCUS       DACDIP010000114         4800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDIP010000114 DACDIP010000000
VERSION     DACDIP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.116"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(51..1199)
                     /gene="nagA"
                     /locus_tag="IV257_002682"
     CDS             complement(51..1199)
                     /gene="nagA"
                     /locus_tag="IV257_002682"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8815745.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1275..2111)
                     /locus_tag="IV257_002683"
     CDS             complement(1275..2111)
                     /locus_tag="IV257_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8815746.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2295..3512)
                     /locus_tag="IV257_002684"
     CDS             complement(2295..3512)
                     /locus_tag="IV257_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8815747.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3895..3978
                     /locus_tag="IV257_002685"
     tRNA            3895..3978
                     /locus_tag="IV257_002685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3929..3931,aa:Leu,seq:gag)
     gene            complement(4218..4796)
                     /locus_tag="IV257_002686"
     CDS             complement(4218..4796)
                     /locus_tag="IV257_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815748.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1454 a         1001 c          775 g         1570 t
ORIGIN      
        1 ccttaaaacc aaacttaaga agtttagttt taaggttaac ttaaattata ttatgcgtgg
       61 aaacgttcca cgccatctag ataagttgct tccaatgtca tatcagggtt caatacgata
      121 aagtctgcat cgcgtccttg agcgattttt ccacatgtat catcgatttt acagctgatt
      181 gctggaatca gactagccat catgactgct tgttctggtg ttgctaatcc ccagtctaca
      241 acatttttaa ttgcttcttt caatttcaat atacttccag ctaagttgcc agattctaga
      301 cgtgctgttc cttcttttac tactactgga aattcgccta aattgtaatt tccatcaggc
      361 atcccaccgg ccatcataca gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc
      421 attaggattc ccgcagcatt tggatgtaca tgatggccat cacaaatcaa ttcagaaaag
      481 acatgttcta aagataataa cgcacccacc atacctggtt cgcgatgatt caatccacgc
      541 ataccattga aagcatggac aaatacactt gcgcctgctt caacagcttt ttgcgcttct
      601 tctagtgtag cattactgtg tcctagagaa acaactactc cttcttcagt cacttgtttg
      661 acaaactctt ctacaccttg acgttccggt gctaaagcaa tttttttgat taaaccacct
      721 gaagcttctt gccattcatg gaaagtatcc aaatcaggat ccccgaaata acttgggttt
      781 tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc tttgatttta
      841 gctcctggtg cttcctgata cattttgccg attgtctcag ctacatcttt taaacgttct
      901 ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc cacaagataa cagaccttca
      961 gacatcactt tgatgccttc tgcatcatta tccattacat catgattcat atagccgtga
     1021 atatgtgtat ctaccaaacc aggagcaatc catttgccgc tttggtcaat gatgttgact
     1081 tctccctcag gtagttcttt ggtgtattca ccaaaaagtc catcggtaat ttcaagatag
     1141 cctgcatgtt tgacacctga cgctaaaaag aatttgtcag catagataaa agttctcatt
     1201 ctattctctc cctttgtttt tctacctcta aagtactctt atttagagaa gaagtcaaga
     1261 atggtctaca ccatttacaa attttttgaa atttgtttca cagcttgtgc taagccttca
     1321 tagaatgctg ctctttctgg ttcttcttta cgcttgtata ttaagctttt aagcacaaat
     1381 aaatcattga ggtaatagta gctattcttc tcttgagccc aatagattcc ttgattcacc
     1441 tgttctaacg cttcgtcgat ttcattttga tggaataaga aatttccata attataaaaa
     1501 atttgcgaaa attcactttt tgatcgttca tcagctgatt gatgcagcag ttggatgctt
     1561 cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca caccatatat ttttccgata
     1621 caactaatca gttgtatttc tgtgtcagaa acatggattc tttctttttg gctagagtag
     1681 cttaatgctt ctttcaaata ataaagggac tgagagatat tttgacgcaa cgtgaattcg
     1741 caaacaccga gataataata atagcgttga aagtcagttg cttgatacag attttcagaa
     1801 atatcagaag attttagtac ttgttctaac ttccgataat cacgtttttg gaaacaacta
     1861 tccaacagtt caaataattt atcgtttgtc cgagtagttt ttcgagagag attcatcaca
     1921 tgatcaatcg ttacccctag ccgttgacaa atctgcatca tcaccaatac attcggcagt
     1981 tcctcattat tttcgatccg acttaataca ctttgtgaac aaatatcatg cgaaagcatt
     2041 ttttgcgtca ttttttgttc ttttcgaatc tctttaatga tcgttccaaa ggactcgatt
     2101 aacgtttcca taagtatcct ccccaaaata tgcgtattcg cattctattc gtattttact
     2161 ttaggaagtc gcaaacttca agaaaagact tgcaactaaa taatatactt tagttattta
     2221 agaaaactcc actagaaaga gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa
     2281 tttggttttt aaaatcattt ttggcgatag gttgctagga attctctcat tttcttagct
     2341 gtttgtttca aatcatccat tttaggaaga taaacgattc gaaaatgatc tggctggttc
     2401 caattaaatc caccaccatg tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg
     2461 aatttttcat catccaaaat attgaatcgc tttgtatcaa tttttgggaa tatataaaaa
     2521 gcagcttttg gttttacggc tgaaagacca ggaatatcat tgattgcatt atagatatat
     2581 tctctttgtt catagatcct tccgcctggc aggagcagct catcgacact ttgataaccg
     2641 cctagcgcgg tttggataat ttgctgagac aatacattgg aacacaaacg catggaggac
     2701 aacatattca gcccttctat atagcctttg acgtgtgact tatctccact taggaccatc
     2761 cagcctactc gaaatcctgc tacacgatga gatttggata gaccgttcag cgtaacaacg
     2821 aacaaatcag gcgctaatgt agcaatcgga atatgttcta gaccatccat cactaagcga
     2881 tcatagattt cgtcggaata aataatcagg ttattttgtc gagctacttc aacgatttct
     2941 tctaataatt cttttggata aagtgcccca gtaggattat taggattaat gataacaata
     3001 gcttttgtcc gactcgttat ttttgatttg atatcatcaa tatcaggata ccattctgcc
     3061 tcttcatcgc aaatataatg aacaggtgtg cctccagcta atgcaataga agccgtccac
     3121 aacggatagt ccggcattgg aaccagtact tcatctccat tgtccagcaa cccctgcata
     3181 cacatcgtga tcaattcgct cactccgttg ccggtgtaga tatcattgat cgtcacattc
     3241 ggaaaatgtt tcagttggca atattgctca attgcttttc tagcagaaaa gatccccttg
     3301 gaatctgagt agccttctga gtcacgggca ttcatgatca aatcacgaat gacttcgttt
     3361 ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca attttaaaat actaatgccc
     3421 tcttcttgca tccgctccgc ttcttctaga acgggaccgc gaacatcgta actgacacct
     3481 tctaatttgt tggatttttc aaattctctc atcacttagc caatccctca tttcaaaaag
     3541 ttagaacttt ccaaattttt tgttatttac tatttcaata tcatactcca tcctagaaaa
     3601 ttttgccact tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac
     3661 ttaattgtca tgatagtcat tccatagatt cgctgcccaa tccattgcaa ttccacaaat
     3721 gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca atacacctaa
     3781 gatcattccc agtcctgaaa attctccgtc atatttctcc atcttaataa gtcctcccat
     3841 ttttgagctt gacaactatg taaaacctcg ctatcattag tgtgttatta ttacgcagat
     3901 gtgttggaat tggcagacaa gcatgattga gggtcatgtg agcgttagct cgtgtgggtt
     3961 cgagtcccat catctgcact acaactcgtg aagttaattt tgcttcacga gtttttttgt
     4021 ttgaacgttt ctttatgcca ttatatacat atcactgtat cctttacaaa aagaacgtac
     4081 aatcatgtaa acagcctgag gtgagtatat gaaggtcgga aacaagctac tcgtagaaac
     4141 ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc
     4201 gagggatttt ttgtatatta tctgtttatc accgttatta taaaggtaca aataccaagc
     4261 acggttaatc caaattgcat aaattcattt ttttgtacac tcatgctttt cattagctct
     4321 ttaactgcca ttagtactaa gatggcgata acaacataaa gtatatagta cgtccgcatt
     4381 ccggaagccc atactgtggg agaaaatccc ataattaacc ttgaacaaaa tcccattatc
     4441 atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa aacttaaata aattccaaca
     4501 attaaacata gtaataatgc caagactact aaagtagcta tccaagtacc tggataaaaa
     4561 agtgacagtt tcgttcctaa ctctgtaaag agattattca ggttactact actccaaatc
     4621 attgcccttt tatttccatt gacgtaagta aagctttgac ccattgtatt accaaaataa
     4681 ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac gcacataata gttttgacat
     4741 ttcctatatg ttagaaggaa aattaagaaa aatagcaata aaaataagat attcatgtct
//
LOCUS       DACDIP010000115         4730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDIP010000115 DACDIP010000000
VERSION     DACDIP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.117"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IV257_002687"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IV257_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8815749.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2887..3192)
                     /locus_tag="IV257_002688"
     CDS             complement(2887..3192)
                     /locus_tag="IV257_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815750.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3337..4542)
                     /locus_tag="IV257_002689"
     CDS             complement(3337..4542)
                     /locus_tag="IV257_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8815751.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
BASE COUNT         1490 a          874 c          627 g         1739 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagtg tttgcatcaa cacttgcatt accgaagtac ttattataga
      181 catctgaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcgataagtt tagttaaatc attattagtt attgaaccta
     1141 tgccataatt ttcatgacct tcccgtcgct cgattgctaa atcagctact tttgttccgg
     1201 attcaaacga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagatga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggtttc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta ggctcttttc aaacgtaatt accccattag
     1681 gagcggtttg cgtatcggtt gtttctgccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggc
     2101 tgcttggaga aatttcatct tgtgctttaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaatcaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa taattacttt cctagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa ccttatgtta actaatttac ttagagaatt tcatgaacat
     2401 tacaacaaaa aaaagtacat ttcttcatgt ttttttcgga tatttgtgct ttttaaccat
     2461 tcagcagctg aaaacatata aaaagatatg tatgtattct ttctgaaatt aagttcattt
     2521 ctatgcgatg aagcatgttt tcaaaatatt actctttctt agattaaact tcagagtatt
     2581 caaatagcgg aataaggagc cttatcccgc attactgaat gagactgtga cataagtaaa
     2641 aaggtgactt caaattcctt cttcggaatt tgaaatcacc ttctttataa ttataaagta
     2701 tcggtttcac agcattcctg aatgattcct agaatcttaa acgaatgatt ggagttatca
     2761 acattttttc actcgttttt agcgaaacaa aaaatgacat ttgtcacagc ctcattaaat
     2821 tattgattct ttttctttat aattgaattg tccatattta aagtgtctag tactctacat
     2881 gaataattaa ttgctttcac tcaaatactt ataatctatt aacttgtagt tgtttaactt
     2941 ttctgaaaaa gttagaccca cgtaataatc ttcccagctt tctctttcaa ttaactgatc
     3001 caatttttga taatccataa attcatcatg tattaaaaag aataaataac ttgatatttt
     3061 agcatctagc ttaatcagat taaataatgt ttgcttaaaa ttgctaccat taatatgact
     3121 gatagattct ctagcttctt cacataaact ttttacttta ttaattacta ctttgtcata
     3181 ttcatttaac acactattat taccattact tagacgctca gattctacga aacaactcaa
     3241 atcagaaatt tcccaaaaaa ttttttgtta aaatccaatt tcctcaactc ctccaaacta
     3301 cgtaaatgat atagtctcat aataaaataa aacctctcag ctaatgttgt tgaaattatc
     3361 cactaaacct aattgatatt gcttaggaga tacacctgcg tactttttga atactctcga
     3421 aaagtgtgaa gaatctgaaa atcctaaacc taaagaaact tcactgatag tatggtttga
     3481 tttcaacatt attttagccg tagaaatttt tcgcttagag atataattat aaatcgtaat
     3541 atgcatttca ttcttaaagc tttctcttaa tttagtttca cttacagaaa aataattagc
     3601 tatttccttt acagtaattc ttttatagat atttaagccg atgaattgaa cgacagaaga
     3661 aattaaggga gaaagatgct ctatcttagt ttttccgatt tccttagtat aataaacaat
     3721 cccagaatcc ctcactttta agacctcagg taacgataca gctaattcgt tttcttgtat
     3781 caaagcatct ctactttgat acgcttctat tatatccatt cctaatgtta tagacgtttg
     3841 tgctaacttt tcaaaaacaa tgatagaata atttttttca gaccgaatcg tatctccact
     3901 aacacttgga attattccat tactcagatt aaaaaccatg ttttctaaat ttcgaatatc
     3961 tccattttta acatattcca gtattttatt ttcataaaga aacagatata tttctggatc
     4021 gtaattttgt gataacagat tatctatttt gatttgctcg atagttttac ttagatttcc
     4081 tgtatattca attaacggtt catggaatag atcttctatt tttccagtaa aaaaataatg
     4141 aactaaaatc agaatatctc gaatgtctcc taaagaatac aaagtcaaat tttgcaatga
     4201 attatagagt cgttctctat cagccattgc aacattcaaa ttgttcattt ttagtttaaa
     4261 aaaatcttta tcaatattat ttgctctaaa tggaccaaat aagtaatatc tggttttaaa
     4321 catgtataga agaaacatct cattaaaatc accagtaaag aaatggaaat ctttagtttt
     4381 tttaatatta tcagttagta actttttata atcataaaaa aaataagtag ttctatctga
     4441 gcgatactct tttataatac tgaattcgga attaaaaacc acgataggta actttaaaca
     4501 aacatgaaga gactttaata agacttcttt atctgaaaac acttttgttt ctctctttcc
     4561 tttctacatc attaaatagt attttatacg ttatctaaaa tactattcct ctgtcaaaat
     4621 aagctaactt acttcattat aaaatatcca cttatcgctt tcatgtatat tccaccgtaa
     4681 aaagtacatt ttatcaccga gaactcatca tttttcatca tgatgaatac
//
LOCUS       DACDIP010000116         4631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDIP010000116 DACDIP010000000
VERSION     DACDIP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.118"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(3..1451)
                     /locus_tag="IV257_002690"
     CDS             complement(3..1451)
                     /locus_tag="IV257_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8815752.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(1451..2167)
                     /locus_tag="IV257_002691"
     CDS             complement(1451..2167)
                     /locus_tag="IV257_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8815753.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(2148..2498)
                     /locus_tag="IV257_002692"
     CDS             complement(2148..2498)
                     /locus_tag="IV257_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8815754.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2642..3415
                     /locus_tag="IV257_002693"
     CDS             2642..3415
                     /locus_tag="IV257_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8815755.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3586..3952
                     /gene="ssrA"
                     /locus_tag="IV257_002694"
     tmRNA           3586..3952
                     /gene="ssrA"
                     /locus_tag="IV257_002694"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4257..>4631)
                     /locus_tag="IV257_002695"
     CDS             complement(4257..>4631)
                     /locus_tag="IV257_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8815756.1"
                     /translation="EGRKAVDIENSINEWLQSVLKNLFKSITFDCGKEFSNWKSISNT
                     NDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMDFHQVNQGFISSVASKRNHIPRK
                     SLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         1364 a          900 c          825 g         1542 t
ORIGIN      
        1 ttttactctt ttttgatttc tacgtcaggc aaacgaacag cgaattttgt tccttttcct
       61 agttcgcttt ctacttctaa ttttccttta tgcaagcgta caagttgctg cacgattggt
      121 aaacctaatc ctgattcacc atattttgta ttttttcttg aaggatctac tttataataa
      181 cgatcccaga tacttttcat ttgttgttca gacattccaa ttcccgtatc ttcaatcgtg
      241 atgatcgttt caagatagcc tttttctaaa gtaatcgtga tttggccgtt ttcagtaaac
      301 tgtatcgcgt tttgaataat attgaccatg atttggacaa atcggtcata atcagcgtaa
      361 acatcaatat catcaacggc tttcaatgtc aatgtatctc ctgctgcttc tgctttagct
      421 tctaactgtg caacaatatt tttcaatgtt tctgttccat tgaatttttt aatgaccata
      481 ctgatttgat tcgtacggat tttttcataa tccaagtttt cattcaccaa acggatcaga
      541 cgctctgttt catttttcat caatttaatc gcgttttctc gctgattttc agggattgca
      601 ttgtattcta acccttccag aagtccgttg attgtcgtaa gaggtgttct catctcgtga
      661 gaagcatctg ccataaattg tcttcgacga ttttcttgtt cttcaatttc tgcttgagat
      721 tctttcaatg aattcgtcat tttattgaaa tcatctgcta actcatcaaa ttcatctttg
      781 tcatgtacgg ggagctgtac atcaaaatta ccattcgtca cttctttcgt cgcattcctt
      841 aaccgattga tacgcttaac ttggaatgct gcaaaagcat agctggcaat aactgcaacg
      901 atactggaaa aaataaatcc tttgaacagg ttcagtgtaa ttggacgaac gctgttatcg
      961 atatttcgcg caggttgact aactacaagt gctccataga attcatgatt caaattaaat
     1021 ggcacaagtg cgtaagaggt cgcctgtgct tgtcctaaaa tatttttatt cgatgtgaat
     1081 ttttcttgac gtccattcct cagatttttc cattgatcag ctgttaaggt aaaattcagt
     1141 aaagctgtat tagtcggata aatgacacgt tcgtttttat cgataaatat aaagttcaca
     1201 ttttgttctg tcagtacttg ctctgtcagc agcaatgcat tttgaaacgc ctgatcttga
     1261 ctaaactgcg ggagcgtatc ggcataggtt tgcgttgtct tttcaactga ttcagcatat
     1321 ccaaacagtt gctggtaatt gttttcttca atcgtttgtt ttgtcagttg tgtaaaagaa
     1381 actccaacga tcaataagat caacgcgacc attccgcaaa aagctaagag ttgctgatac
     1441 aaataacgca ttaggcaaca ccagaatcat cgaatttgta gccgactccc caaacagttt
     1501 ggatgacttg tggtccgacc ttttcgattt tttgtcgtaa tttcttgata tgggcgtcca
     1561 ctgtccgttc atcaccaaag tactgataat cccagactaa ttcaagcaac tgctcccgag
     1621 agaatacttg acgtggtttt tttgctaaag taaacaatag atcgaactct ttaggcgtca
     1681 gtccttcaat tggccggttg tccagatacg cttctcgtgt tttcgtattc atcttgaaat
     1741 gactagtctc gatatcaaaa tgatcatctg aaactgctgc ttcttctctt gcttctgcca
     1801 actcactacg gcgatgcaaa gctttcattc gagcaataag cgtcagagga ctgaaaggtt
     1861 ttgtgacata atcatctgcc cccatctcta agccgatcac ctgatcgctt tcagaatctc
     1921 tagctgtcag catgataatc ggcacagtac ttgaaacttt gcggatctct cttcctacta
     1981 gcataccgtc cagtgtgggc aaattcaggt ctagtgtaat catatcccaa tgttgtgggt
     2041 tttctaaaaa ggcatcgagc ccttcttttc catcatactt gaaagttgct tcccaacctt
     2101 catttaagaa aaacatctgc atcatttcag aaacagattg attatcttca atcattaaaa
     2161 tattcattat cgttccctcc agtttccagg tcatatttct gtaatttctt ttctgtcata
     2221 ataaaaatag gcataattgg aaatactagc agaagcattt caatcagaaa ataattcgta
     2281 tgcccagatg tctttaaata atagccgatc aatgccatca gtaagcccat aagaaaaaac
     2341 cagtttccgc ttgtcttttg agccagccgc caatgagcgt ctgtttttgc ggcaagaggc
     2401 gaatgatacc cataaaaagg cagatttcct tttgagggca gaatccgaaa aagactgcca
     2461 attattaaga ataagatacc aatactgaaa aaaatcattc tttccctcct aaattctctt
     2521 cttatagtat actctaaatt gttgactgaa ccaatatgtt caggaattct tcacgaaagg
     2581 tttgtctttt gttcattctt tgttaagata aacttattcc aataaaaaag gagttttatt
     2641 tgtgtcaaat tatcgtgcaa tcactttctt tgatcttgat ggaacattac tagatgcgaa
     2701 atcaaaaatc acacccgaag tggcaaaagc tatgaacgca ttaaaagaaa acaatgttct
     2761 ccccgtcatc gctacaggac gtacagaagc agaaatccac tctatcatgg aagatgcggg
     2821 cattacttct gcggtcacca tgaatggttc tttcattcga gtagacggcg aagaaatcta
     2881 ttctcaaaca ttttcttcag aagaatgtca aaaaatgtat gatcatgtac agttgaacgg
     2941 tcatgaactt tcttactata acgatcagaa aatctggtgt accggtcata gccaaacaat
     3001 gatcaatgct tacgacttca ttcattcaga tgttccggaa attgatcctt taggctttaa
     3061 agaaaacaca gtaaatatgt tgctgatctt atctcaatca ggtgacgaat actatcacga
     3121 acattttcct gagctaactt tttatcggaa tggtccttac tcgatcgata cagtgaagaa
     3181 aggaatctca aaaggggcag gtgtcaaacg tctgcaaaaa gaacttcagc tggaaaaagt
     3241 tcctactttt ggatttggcg atggtccgaa tgattttgcc ttgttggaag cttgcgacaa
     3301 caaaatcgct atggacaacg cctatgacgg attaaaagaa ctttctacct ttatcacaaa
     3361 gaaaaataca gaaggcggaa ttgtccacgc attgaagtat tttgatctga tttaagatct
     3421 ttttttgaag ctacctcttt tcttaagaaa atcgcttatt ttttcttaag aaaggggcag
     3481 ttattatata aataatggga tatgattcgc ttttttattg aatttatccc aatatatgct
     3541 atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga cgttacggat
     3601 tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt aaaaacgtta
     3661 cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc ctaaaaacag
     3721 ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa ctttagtggg
     3781 atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag cgatacagaa
     3841 tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat gaacgtagat
     3901 ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat atatgtatgg
     3961 ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt attttggtca
     4021 aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc aaaaaaacat
     4081 tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg tttacaaaaa
     4141 gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa ggaaaaacac
     4201 aattatggtt taagcatgtt ccttcaaata agttttttta attattattt gtcaaattaa
     4261 gcgagacaaa actttccatt tacgtaactc aaaaaaactt ctaatggtgt ttggtaattt
     4321 agtgattttc tagggatatg gtttcttttg gacgcaacgg atgagatgaa tccttgattg
     4381 acttggtgga agtccatttc ttttggtagt ccatcttttc ggaggagtcc gtttgaatgt
     4441 tcatttaatc cccgttggga aggcgttcct gggtctgcga aataaatatc aatatcattc
     4501 gtattactaa tacttttcca attagagaat tcttttccac aatcaaaggt aattgatttg
     4561 aaaagatttt tgagtacgga ttgaagccat tcattaatcg aattttcaat atcaactgcc
     4621 ttacggcctt c
//
LOCUS       DACDIP010000117         4465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDIP010000117 DACDIP010000000
VERSION     DACDIP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.119"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IV257_002696"
     CDS             574..1008
                     /locus_tag="IV257_002696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815757.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IV257_002697"
     CDS             2298..3221
                     /locus_tag="IV257_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8815758.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IV257_002698"
     CDS             3237..3776
                     /locus_tag="IV257_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8815759.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          674 c          757 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
      421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctaa
     4261 acggtcggcg aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DACDIP010000118         4454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDIP010000118 DACDIP010000000
VERSION     DACDIP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.120"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..580
                     /locus_tag="IV257_002699"
     CDS             <1..580
                     /locus_tag="IV257_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8815760.1"
                     /translation="FEIRLREQGISKEGQYGKYEGYKPYLRSGQIRYIRGFPILPVGY
                     IQHRHPMCKKRNINKYTEEGREAIHKKQQTVEEWKISWLCSNPVLSKRATIEFADNRI
                     SKFIAQKGRCAVTGEELILSEMHCHHIIPYHESKSDSYENLVIVTEEVHRVIHATQSE
                     TIEELLKYLKLNPKQKEKLNELRLKVGNEEIS"
     gene            711..1088
                     /locus_tag="IV257_002700"
                     /pseudo
     CDS             711..1088
                     /locus_tag="IV257_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            1088..2293
                     /locus_tag="IV257_002701"
     CDS             1088..2293
                     /locus_tag="IV257_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8815761.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            2309..2953
                     /locus_tag="IV257_002702"
     CDS             2309..2953
                     /locus_tag="IV257_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815762.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            2964..3770
                     /locus_tag="IV257_002703"
     CDS             2964..3770
                     /locus_tag="IV257_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815763.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            3792..4022
                     /locus_tag="IV257_002704"
     CDS             3792..4022
                     /locus_tag="IV257_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815764.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         1653 a          707 c          932 g         1162 t
ORIGIN      
        1 ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg gaaagtacga
       61 aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt ttccaatatt
      121 accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata ttaataaata
      181 caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag aatggaagat
      241 atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat ttgcggacaa
      301 tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg aggagcttat
      361 cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa gtgatagcta
      421 tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta cacaaagtga
      481 aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag aaaaactcaa
      541 tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg taggacggga
      601 aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg cacgtacggt
      661 gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta tcgattaatc
      721 cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg cgtaaagtag
      781 aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa aatatgtctt
      841 ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag atctttatgc
      901 gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca atgacgaact
      961 cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat tttggctcaa
     1021 aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg tttgatggag
     1081 gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc gtcgtgcaaa
     1141 gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta ttttgatcac
     1201 tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt cggattcaga
     1261 ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac cgatttataa
     1321 agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg taggtgcagc
     1381 agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg aatcagcgtt
     1441 tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt ggtttgatgg
     1501 ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg gctggtacat
     1561 gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag ccacacaatc
     1621 caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga tcttaggacg
     1681 aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag gttgtatgtc
     1741 aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac attggggtga
     1801 aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc ctggcagcag
     1861 tttagataaa agttccttca gcggtggtca attatttgga acgaatccag gcggtgaatt
     1921 ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc caggaagtga
     1981 gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta tttcaggttt
     2041 gggcgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc aagaatttgc
     2101 aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg gtcaagtaat
     2161 tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt ggcgtcaagc
     2221 acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat ttttgaatag
     2281 cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa tcgtcttaac
     2341 agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc tgaaagagga
     2401 acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg agaagctcaa
     2461 aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca tcaatgagaa
     2521 aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag aggaagtaac
     2581 tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata aatcattaag
     2641 cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga tcgacaaatt
     2701 aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct ttacattagg
     2761 tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt ttgtcgtatt
     2821 tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa aaataaaagg
     2881 tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag actttagtta
     2941 tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa cgtttcaaaa
     3001 aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt ttgattcttg
     3061 ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc acgaacgcct
     3121 ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac cagcaattag
     3181 tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg gctaatctta
     3241 attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga gccaaagtga
     3301 ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa gaatatatcg
     3361 ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg caagaaccac
     3421 aagatctgat ttattacgtg cacgaagata aggttaaaga tcaagtgaaa gatgaagatt
     3481 atgaacaaca cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa aaagcagctc
     3541 aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta gtgaagaagc
     3601 taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa aacaagatta
     3661 atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa gaagagatca
     3721 ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag aaggacaggt
     3781 ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct gagcagaaag
     3841 tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga gaagtgaatt
     3901 tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat actttaaaat
     3961 tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct gataacaaat
     4021 aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga atcagctata
     4081 tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc taacgcaaac
     4141 atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca accaaacagg
     4201 aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca tatgacaaaa
     4261 tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac acaaataacc
     4321 ttaattttta aagagcataa ggaaacgtga ccccactttt aaaaaaagtg gttggatagg
     4381 ctgtgcctat cacgtttctg aaaaaaataa atgaccccaa aataccaata tataggatag
     4441 tacaaggata gcaa
//
LOCUS       DACDIP010000119         4374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDIP010000119 DACDIP010000000
VERSION     DACDIP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.121"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..641
                     /locus_tag="IV257_002705"
     CDS             <1..641
                     /locus_tag="IV257_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP8815765.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(727..1821)
                     /locus_tag="IV257_002706"
     CDS             complement(727..1821)
                     /locus_tag="IV257_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8815766.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            2010..2771
                     /locus_tag="IV257_002707"
     CDS             2010..2771
                     /locus_tag="IV257_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8815767.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2886..3326)
                     /gene="fabZ"
                     /locus_tag="IV257_002708"
     CDS             complement(2886..3326)
                     /gene="fabZ"
                     /locus_tag="IV257_002708"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8815768.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3546..4301
                     /gene="fabI"
                     /locus_tag="IV257_002709"
     CDS             3546..4301
                     /gene="fabI"
                     /locus_tag="IV257_002709"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8815769.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         1415 a          850 c          762 g         1347 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata acctataaaa
      661 aaacaaaata aaaaagcaga aataactggc tatttctagc ggattatttc tgcaataact
      721 aattatctat tttttatttt caattaattt aaagcttgac catggtttca aaaagtcaag
      781 gaggaataat tcgtcatcgg agattcttcc tacaacattg acacgtccgt cgtttttcat
      841 atccttcaag gcgatttgtg tttctccttt gtattgaccg tacccttcat tgtcaatcag
      901 tacatcccca tgatgcatat ctcgcgtatt atgaggcggg aaatctttat ctttgtagta
      961 tacacgagtc atcgttgaac gaaggatata ttctgagcga tcccctcgat aactatgaag
     1021 gttatcaaac aagcagattc gttcgttttc tgtaatacct tcttccgtat ctactttcaa
     1081 tgttggataa tccgcgttaa atgcttctgc catttctttc aattcttctt cacttgcgta
     1141 cgcatttcca atagaaatat catcaatcaa tcctgtaagg attaaatgct tcacttgtgt
     1201 cgcaatttca agatctcggt ggtcttccag cgtgcacaag ccatcttgcg taggccaagg
     1261 gccaaactca gcagattgcg aattgacgaa tgccatcgta ttcaaattat attttctgaa
     1321 tttttctgaa caaaaaacaa agtgttcgta tcctaaacca gagtatcgat gaggataaaa
     1381 attatgagaa cctaataaat tatccgtgtt tggcgaataa ctcatgatgt tgtcaacgta
     1441 attcgtgccc gaactcatat tgatctcgat tttgatacca aaaggattgc gcgtcatttt
     1501 tgcttcttct gctcctgtga atccgatatc taagcggacc ccgtaggcac ccatcttatg
     1561 gaaaaaagat aagtcatcgt aagaaatctc caactgttca aataacgctg gattgatatc
     1621 aaccattact tccatgccta ggctattcgc gtaatcaact acttctttaa attctgaaag
     1681 aactttttct ttatcatcat tgatctgcag caaactggta aaaacccgtt tatacccata
     1741 cttatgagct aaatccaagt acgctttgtc cttttcaaat gttgaccgtt ctggataaat
     1801 cgagattcct aattttccca tattaccatt tcctcctgca tcttttcgtt tttgtatcca
     1861 cttacattat agcaaacaca aagtaaatgg tctacacctt ttctttttct tttaaaataa
     1921 atgataaatt ccaataattt aagtaggtat tttccctcta ttttgttatc attaaagata
     1981 acaaaatagt aaattggaaa ggaacagtta tgaagaaaaa taaagttatt ttaagtatta
     2041 gtttagcagc agcattgctg tttgctgctg gttgttctgc aaacaacacg acttcttcta
     2101 gttcgtctac agaaagttca aaaagtacat ctactgcgga aagcacgact gccagtagcg
     2161 tcgatttgaa cgcattggaa ttaccacaat tgacgaaaga tgtcgccgaa aatgaagatc
     2221 tggtcgaaat ggtgacgaat aaaggcacaa tcaaaatcaa attatttccc gaacacgcac
     2281 caaaagcggt tgaaaacttt atgacacatg caaaagacgg ttactacaac ggactgactt
     2341 tccatcgcgt catcaaagat ttcatgatcc aaggtggcga tcctaaaggc gatggcactg
     2401 gaggggaaag catttgggga gaaggatttg aaacagagat caacaaccac ctttataata
     2461 taagaggtgc gttatctatg gctcgctcac aagatcctaa cagtaacggc agccagttct
     2521 ttatcgttca aaatacagat gatatgcatg atggtctttt aaaagacgac tatcctcaag
     2581 caatcatcga cgcttataaa aacggcggtt atccttcact agatggccat tatactgtct
     2641 tcggccaagt gattgaagga atggatgtcg tggatgctat tgcaaatatc gaaacagata
     2701 gttcagacaa accaaaagaa gaagtaaaaa tcgataaaat caatatactg caagaagcaa
     2761 aacaaaaata aaaaaataac gtctgaatcc tgcatagatg acaacgtatg ctaggattca
     2821 ggcgtcttct ttttacataa aaagcaagta agcttccttt tgttttgcta tatcaggttt
     2881 tatagtcaag cattttggtt gacgatgaac gtgaactcgc actcacagac ttttttatca
     2941 tctacatatg ctgcagataa cgctgtaccg actgatccct tcattttggt gatttcaaaa
     3001 tgacatttca gcacatctcc tggaacaact ttttgacgga atttggcctt attgatccca
     3061 ccgatataag ctctttttcc tagaaactct tctgatttta ggataagtat cgaacctgct
     3121 tgtgccagtg tttctagaat caacgcaccc ggcatcactg gattgtcagg aaagtgacct
     3181 tggaaaaaat cttcattgat tgttacattt tttgtcgcaa cgatcttttt tcctggttct
     3241 aaagtatcta cataatcaat ataacaaata ggataacgat taggaatcaa ttccattact
     3301 tcagtagcag ataatacgta agacataatt aaactccttc tttcaggcaa ttgccatttt
     3361 cattactttg actatcaaag tatcatattc ctaaatattt agcaatcttt ttttaattta
     3421 agaaaaaata gtttgacaat caaaacattt tgactaataa ttaagaaatc aagaagttat
     3481 tttagctatt attctctatt tttttacgac tatcaaattt ttatcagaaa caaaggagag
     3541 tcattatgtc atttctagaa ggaaaaaaaa tcgtcattat gggcgtcgct aacaaaaaaa
     3601 gcattgcttg gggttgtgct gaagcaatgt tgaaacaagg ggcagaaatt atctatactt
     3661 accaaaatga acgaatgaaa aaatcattag tcaaattggt cggcgaagaa gcattcactg
     3721 tagaatgtga tgtttctagc gacgaaagca tcgaaaaagc aatgaatgcc atcggtgaat
     3781 atgctgggga gatccacggc cttgtccatg cagttgctta cgccaacaaa gaagaattgt
     3841 ccggaaatgt gtctgatatt tccagagacg gctatcaatt agctcaagat atcagcagct
     3901 actctttaat tgctgtatca aaatacgcac aaccgtttct tgcaccaaac agcgggatcg
     3961 ttagcatgac ttatctaggt tcagaacgcg caatcccgaa ttataacatg atgggtattg
     4021 cgaaagcttc tcttgaagct gctatccgtt atttggcagc tgagttctcg cctaaaggaa
     4081 ttcgggtcaa tggaatctca gctggggcaa tcaaaacatt agctgtcaca ggtgttaaag
     4141 actatcaaaa actgatccag ttatctgaat ctcgtacgcc tgatcagcaa ggtgtgacga
     4201 tcgaagaagt cggtaatgct tgtgctttct taatcagccc actttccagc ggaatcatcg
     4261 gtgatattat ctacgttgac aaaggtgtcc atctttcata aacttttgtt gcattcttta
     4321 aagtaaaaca tataaaaatc aggaaaaaac tttttaaagt ctcttcctga tttt
//
LOCUS       DACDIP010000120         4372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDIP010000120 DACDIP010000000
VERSION     DACDIP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.122"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(83..1051)
                     /locus_tag="IV257_002710"
     CDS             complement(83..1051)
                     /locus_tag="IV257_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8815770.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(1496..2902)
                     /locus_tag="IV257_002711"
     CDS             complement(1496..2902)
                     /locus_tag="IV257_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8815771.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSENEIEW"
     gene            complement(3124..3486)
                     /locus_tag="IV257_002712"
     CDS             complement(3124..3486)
                     /locus_tag="IV257_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815772.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3490..3798)
                     /locus_tag="IV257_002713"
     CDS             complement(3490..3798)
                     /locus_tag="IV257_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815773.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3807..4226)
                     /locus_tag="IV257_002714"
     CDS             complement(3807..4226)
                     /locus_tag="IV257_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815774.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4219..4359)
                     /locus_tag="IV257_002715"
     CDS             complement(4219..4359)
                     /locus_tag="IV257_002715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815775.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1241 a          979 c          604 g         1548 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tactattcct cctctgtcac atcaagtccc tgtaaccaag
      121 tatccaattc tttttttaaa tcatctgctg attcttcaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcgcttta ataaatttcg atatttatat ttcgagtttc
      241 ttgacaaaat cgcccaatta gaatgattag ctaacaatcc atcaatcata actttatccg
      301 taggttttat atcagttggt aaaaaatgtg gactcacaaa gctatccaaa ctttctttca
      361 ccatagcata ccattccgta gctttaccac gtcttaactg catttctaac cgccaccaag
      421 tattaatttc tgaaggcacc acctttcttt tcatttcttg ctctattttt ttattgtaca
      481 aacgaatttg ccgctcgctt gcccgactac cccaatacac cgtttccagt tttcctgagc
      541 gtccataaat taatttaaat gaaactggat caataattcg atattgcgtg ataaactcat
      601 caggcacatc aataagatcg caggcaacat ccgctcgtga aaaatggggc tctaaaaata
      661 aatcatgaat aaaatttttc atggaatccg ctaaaaatcg actgatttta ttaggattaa
      721 aatcaatccg cccttttcct tcttgccatt tagaaatttc aatataggca atattttcgt
      781 catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag ccattgcctt
      841 catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga gtatgataaa
      901 aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa tttaatttta
      961 tagtttgtac caaagtttga acatttaacc gcctgttaga cagggctgtt aatttttccc
     1021 actcattcac gattgttctt tttttactca tattcagttc ctcctcaaag aaaaaagaga
     1081 tttttggaaa gaatttccag agttcacgta actctaaaaa ttcataacca tcaatctcta
     1141 gtcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac tagtgtggtt
     1201 agtttccgta aaggtcgaga acgttggtag cgttgctcga cttatttttt tgctcttttt
     1261 ccagttcatt tgtttgctaa ctcaataata gcacttctct ttagctatgc aaataccaaa
     1321 aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc taagttggcc
     1381 aaaaaatgga cttgccattt tttagcaaat gtcagcttca ggggctagtg gtttagccgt
     1441 caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat cgtggctacc
     1501 actcaatctc attctcagaa cgctctaatt tatgctgtga gacgttttga cgataaaata
     1561 aggttttctc ctgctctaat acttctaatt cctcagaaag ccccatatcc ttttctgcaa
     1621 gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgcgtttttt aatcgctcta
     1681 aacgctggcg taaagagcga ttttcttctt tcagcttttt attttccact tgtaaatctg
     1741 ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca tactcattta
     1801 attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca gcatttctct
     1861 tggcatttgt tgctctcgta gctcgttgat acaaatcaac aaaatcattc ccagataaat
     1921 gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aaccatatct ttttggaaaa
     1981 tgaccgccat ctcttttttt acattcagca tatttttctt ctcttcttca atcttcgctc
     2041 tctcagaagc catcagagcc ttatcacgct cgttttcttc tctttgccta taaatatacc
     2101 tcaactcatc agaaacgctc tcacgctctt ttacagccgt ttggcgcaat tcagaaattt
     2161 cactcatgat ttctttgtat tcgtgaatat ctttaatttt gttggtaccg acaatatccc
     2221 ttttccaacc gtaatcattc attaatcgtt ctatagagtc aacttcttga tttctaaaat
     2281 tattgaaaag cactcgacca ttatctttat cagcgtgtat cccttgttga accagtgctt
     2341 tattaaagct cggctgcttt tgaacacccc gtttataacc ttcagcaacc ggcacaatat
     2401 tcaagtgcag gtgcggactt atttcgtcat tatgaataac agcattataa acagccaaat
     2461 tagggtttct ctcttgaaac tccttgtaat actgaaccaa aattttattg gcaatcactc
     2521 gattttgttc agcttgttgt ttcgatatgc cagtagaaga accatctgct cgatttgtct
     2581 ggaaatcatc taaaatgcct atttgcacaa taaattctcg ttgaggttcc aactttttat
     2641 ccttgcgcac tttcgctaaa tagtcctcga tttttctatc ggctcttttt tgtttcgcat
     2701 tatatttttc taccacttcg ccaaacaatt cctcatattt atctgctaga ttcgtctgct
     2761 ctaaataaat attttcatcc gatttcgtga agtcaatgtg cttatgccaa tcattggttt
     2821 tctggcgatc agttaactca cgattattat gagaaatact tgtattcttc gttccttttt
     2881 taaatgaaat actaaacccc aagttaaaac cctccttcgc gctgtcgcta ataaaaagat
     2941 tgtaaacctt tctgttactc tttgtcaaag agtaacattt tcctaattgt gacatggatg
     3001 tcacaattcc aaacgctctc cgagggtttt cgctgtcaca agtgacaacg aaaaaaggag
     3061 ccgaaagcgt atcacgctat caactccttt tatgctttat tttgcttcac gcaaaataat
     3121 cgcttatcgt aaattcacgt cattaaatat tgacgtaaat ttactctcac tcatctgact
     3181 tttcttattc ggtatcactc cgaataagtc gtcagattca tcgggataat ctcgttcgct
     3241 tggcgctgat ttttcatcag caagccactc attatccact gttttttcgt gttgctgatc
     3301 gtcagaaatt gacgataacc accgctcaaa atcagcaaaa gtttcaaacc caaaacgctt
     3361 gcgaagagcc ttgcccattg cagcattttc tttctcaatc aagcgttttt tctgttggcg
     3421 aagtttcgct tgttcttttt ttaatttttc ttcttgcgct ttaatatctg ctaaagtcat
     3481 tcccataaac tacatctcct tttctttgga attaataacg gaaatttggt ttaactgcat
     3541 aacatgactc attaatttca accaaaattc tttttctgat tcatcagttg tttgcgctaa
     3601 atgcttatga acaaattcat tagtcaaaac tgaagatttt gctagatcag cattgataac
     3661 cgagttgaga acatccataa ttcgttcaag catttcacta taattatctt catccggcaa
     3721 attcaaataa tcagctaacc gttcatacat caattcaatt ctatcgactg tttcttctct
     3781 caaatcaatt ttatccatgt cacacctcac ttccagaatt gccaccattt tcgatttttt
     3841 aattcttgat tttctagtaa caactgatcg tttttttgtt gctgaagcaa agtcaatttt
     3901 tgttggtcac gaatcatttc cgttaattca gaaatatgcc tatctttttc cgctaattgt
     3961 tcacgcaaaa attcattttc tttttctaac gggaaatcat tgcctgcatc attgaccggt
     4021 aaatcaccag tcaattgacc accttttgac cgataaattg accgcacaac accaatcaat
     4081 tccttgtcta acatgataac cccattatct ttataccgag caaggtcata acctgcattg
     4141 accagttgat tgcgcactgt atttttagat atacccaact catcagcaag gttttttatg
     4201 gtcatttttt ctggcaaatc agtcattatc ttctccgtta ctttcagcta ccccataaac
     4261 atatttaaca gcatgcttag ggataatgat tggggaatca attttattat ttttcaaaat
     4321 caactcatac tgcgttacat caagtaactt accggacatt tttttgctat cc
//
LOCUS       DACDIP010000121         4353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDIP010000121 DACDIP010000000
VERSION     DACDIP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.123"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            38..2092
                     /locus_tag="IV257_002716"
     CDS             38..2092
                     /locus_tag="IV257_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815776.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            2092..>4353
                     /locus_tag="IV257_002717"
     CDS             2092..>4353
                     /locus_tag="IV257_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8815777.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIED"
BASE COUNT         1669 a          676 c          869 g         1139 t
ORIGIN      
        1 tactttttac tccttttttt atgaaaggag aatattcatg ttttttacga tttcaggaaa
       61 tgcaaaattg gataaagagt taataagaaa agcaacgaag aaagtgggaa agcgtacttc
      121 ctttttaccg aaaacaatta aatattctgt cattgaaacg gaagaacagc tacatgtgtt
      181 aggacaagtg ttagaacaag agaatcaatc ttctgttaaa ttgactctca cgttacatga
      241 taaagaagga aaaatagaaa aggctggcac agaatacgtt tttggcccac tggattatca
      301 aatttcagaa ggtttttatc ctacattgat agatttaatc cgccttgatg tgtttaaaga
      361 tacggatgaa actatgagtg tggaagagtc ttatcgaaac gaaaaacttt tacaagtttt
      421 agaagaagag ttagagagcg accaaagaaa agtgaatcaa gtgagtgaag tcgaagtaaa
      481 tgaagaagta ccatgggaac gaccaatctt tccaaagaag gagtcaaaag aaaaagagac
      541 agtctatgtt gacgaacaag ctgatattcc gactcagaaa tcgatatttg aggaagagaa
      601 cgatgtgatg ggtcaattgg aggtagatcc ctttgaagaa acaatggagg atgaagagac
      661 ggacgaagta gaagttgatc cagttgaagc taatgaagag tcaacatgga ctgaagggga
      721 ttttcaggaa tatattgaac cacttatcag tgaagaagta gaaaaaaacg aaacagagat
      781 acaaaaggca actgtatttg attatatttt ggatcaatat ccgccagagc atgaatggat
      841 gaaagaaaaa aacaaattat ttttaaatga gctttattcg aaatatcaat taccccaaac
      901 ctatcaagca tttttagaga gtaaagaaga attaatctca cagggtaaag gcacactttt
      961 agaacagtta gagtctgtcc aatcaacaaa ttggcatgag cttgcacaag aagatttatt
     1021 agccaccttt gaagaacgtc aacaagaaac agatcaagaa attaatgagt atgcgacgga
     1081 acagtcgtct aattgggaga aagtaaaaga ggaactaaat aaagaagaac agcgagtgat
     1141 tgaagaagaa gtacaaaaag tcaaagagcg gtacgaaaag aaacgcgaca atgcttattc
     1201 cttgtgtgta gaaaagatca acaattttac taagacgaat caagaagcgt tatcgaaaga
     1261 aaaagaaaac ttgttaaaag aacgtattaa agaacgaaaa gaagagttct accgcggttt
     1321 acgtacagat aagctaaaga ttagcaaaga attaaatgaa caactgaata agctatatca
     1381 aacaacgact gaagttctta tggaaaaaca cgaacagatc cagcaagaat tacaagtaca
     1441 aattccaaaa tggaaaaaag agtatgaaat tcaacaacaa gaattgaaag aacaagaaga
     1501 aaaacaacgg caaaaagaaa aagagcgtga acaattagaa ataagaaaac gggaacagga
     1561 tcttgcggaa caaaaaatgg cgatagaaaa acaacggtta gagcaagaaa aggaaaaaga
     1621 agcagctgct cgtaaggaac gtcaagaaca aatggataca ttgcgtcaag cacagttgaa
     1681 ttatctagca caacctcaac cagtagctgc tgttcaaacg ccagtgaaca accaagcaaa
     1741 tttaactcct tcaccccagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt
     1801 aagcttgctt ctaggtggag gaacagtatt tgcttttaat catatcaatc aatcttcaca
     1861 ggctgattct gtagccacaa cggcgtcaat caaggaagaa gcccaggaac aagcaagaaa
     1921 agagtatgaa caacaattag aagaattatt gaatcgtacc aatcagtcaa caacagagtc
     1981 aagcatggaa aatagcaaag agagtgattc tagtaaggct aaagaaggat caagcgattc
     2041 aactgtacaa tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt
     2101 agagaaacaa cgaagcgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct
     2161 catttctatc gctatactcc ctcaggagaa gagctaccag aaaaacatga caaacgcatt
     2221 tcgaaaaaga ttttttatag tggacttgta tacctccctt tagagtattt agtaacaaga
     2281 gtaccagaag ttttcaaccg attggaaatt gttaagggaa gcttacaagg aataaatctg
     2341 tttcaattac aggaaatgcc acaggtcttt cattttgatt cagctggttt ttggaatcca
     2401 tttactccta caactagttt gggtacacgt ttagcaattt cgacagtttg tgtggttcca
     2461 gctgtactta tgggattgaa aatggacatg tggtttcgtc ctctagcaga tggacaaaaa
     2521 ggggataatc gattaatgac cttaaaagaa atccaacaga tttatccgca aatacctgaa
     2581 acaaaaacta cttttccagg ttatggaggt attccaatca cacattacaa aaaatattgg
     2641 tacattcaaa cagatactgt taatacgtgt attgttggta cctctcgatc agggaaaggt
     2701 cagattgaag tgttagccac cattgacaat ttatctcgtg cagaaaaaca aagttcaatg
     2761 gtcgttaatg atccaaaaac agaattatat gttggtagta aagatgaact agaagctaga
     2821 gggtatgatg tatatgcctt taatatcacc gatccactgc aatcaatgag tgataatcca
     2881 ttggcactca tcgtgaaata ctggaaacga ggagacatag atactgcaac acagttaacc
     2941 aatactttca caaatacgat ttattatgat gccaacgctt cagataacaa atactttaac
     3001 gaaaacgcgc agaaagctgt caatgcgctt atttttgcgt tgttagaaca agctgatcaa
     3061 acaggagatt actccaaatt gacaccgaat aatctcgttg agcttctttc tgagattggc
     3121 ggttttaatt attatccaga tccagacaat gaattcaaac agttgaatgc cttagatgaa
     3181 tttatgaatc agttgccacc tggaaacatt gcaaaaaaac aatatggggc aacgaaaatt
     3241 ggatctgata aagcgaaggg aaatatttta tcaacggcaa tcacgggatt gaatccattt
     3301 actctaacga aaattgcaaa gatgacttct caatcaacga ttgattataa atcggttggg
     3361 tttccaaaat acttagattt gaaattacat gaaagtcttt taaatcaacg agtcgtagta
     3421 gaattttatc ataagggaaa gaaatttcat gaagaacgtg tcaaagtagg ctataaagga
     3481 tttagtgaaa tcaattttga ttgtcattta gttgcaggtg atcaagtttg tttaaaatac
     3541 gatcatcaag gaaaagaata tgtggcttgg tatgaatttt ctcaaagagt attgaaagac
     3601 gaagagggta atatcgttta tcggaaaaaa cgaggagaaa cccatttgcc agaattagaa
     3661 aaagaagccg ttctaacatt agatgaggaa aaaagtaact tattagtaga gtctattcgg
     3721 atgcactata ccgataaacc aatggctatt tttatgctta cacctgatta tgatccatct
     3781 aaccatatca ttgttagtat ttttcttagt cagttatata aagaattatc tacgcaatgt
     3841 gttaagacta aaggagataa atgtcaccgc aggattcatt ttattttaga tgaatttggg
     3901 aatatgccac caatggataa tatggaagga atcatgacgg tcacagctgg ccgaaacatg
     3961 ttgtgggatc tgtttattca atcttatcag caactttatg cgaagtatgg agaacaaaac
     4021 gcagcgatta tcaaagaaaa ttgtcagaca catatttata ttatgtcgac agatgataat
     4081 accatcgaag aattttctaa aaaagtgggg aataagacgg ttgaacaaga gaacagtacc
     4141 atcaattcat taggtatgaa tactcatatc aatcgcaatg ttgattcaga tcgtatctta
     4201 actcctgaaa gaatttctac ttttttagaa ggagaaacga ttgtactcgc tcctttaaaa
     4261 cgacaagatt tgaaaggtca taaaatacgg ccgtatccta tttttaatca tggggaaacg
     4321 aatcttcctt atgcttatca atatattgaa gat
//
LOCUS       DACDIP010000122         4165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDIP010000122 DACDIP010000000
VERSION     DACDIP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.125"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            471..1964
                     /locus_tag="IV257_002718"
     CDS             471..1964
                     /locus_tag="IV257_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8815778.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2099..2395
                     /locus_tag="IV257_002719"
     CDS             2099..2395
                     /locus_tag="IV257_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8815779.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(2419..3378)
                     /locus_tag="IV257_002720"
     CDS             complement(2419..3378)
                     /locus_tag="IV257_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8815780.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            3561..>4165
                     /locus_tag="IV257_002721"
     CDS             3561..>4165
                     /locus_tag="IV257_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815781.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT         1377 a          669 c          805 g         1314 t
ORIGIN      
        1 ttcagtatat cagatatatt caaaatgtca aaatatattt gtgcgttttg cgttgacaat
       61 ttagagtgaa aagaggtaaa cttaattcta tagaaaccac agataattca ctatttacaa
      121 agtagcaaaa gcagtggtac tctttaaaca gaaaaattac ataaaaaaaa gagacggaaa
      181 taattgattt tagcagaatc aattatttct gaatcacatg gttaggacat gtgttcacgc
      241 tcgtctcaag aatatgtaag tagctaccaa ctacttatat taaaagttga tcggatacct
      301 tttccgattg cctttattct accaaaaaaa ggcgagcaac tcaacagaag aacgtttatt
      361 tagtgtactt aaattaaata ttcttaaaca ctgaagagag aacacatact atggctgaga
      421 ttaaagctaa actgtgttct ctttttgttt gagaaaggac gttacaatcg atgttgagcg
      481 aaatttattc agctattctt aaaaacggct tacgtacata caaatatcga aacagcacca
      541 tgaaacctgc aggtcatatt gattccagta ggagtggtgc tatctttgcg taccgtagca
      601 agcaattaat gaatgttggg cgtggtatgg tcattacttc ggaagaagct attttagaga
      661 acgaaaagaa actaacgcat tggacaccta atacctatcg ctttggtact tatgcggatg
      721 attatcgaac agtggttaaa ggtcattctg agaaaaactt aagtcaaatc aacacatttg
      781 tagttgatat cgattcaaaa gagaaccatc aaggcgaaat tattttagcc tgtttagatc
      841 aagtgggcta catgccaacg ctaattttag aatcagatca tggttatcaa gtttattttg
      901 tactaaagac accaggctat gtcacaaaga aaagcaattt caaggtgatc gatgtagcaa
      961 aaaaaatttc aaaaacaatt cgtctacagt tggctgaaaa attgtccgga gtggatctag
     1021 gatgtaacca cttcggaatt gctcgattcc cacaagaaaa taatattgtt tttaaagaaa
     1081 tgaactatca gtattctttt gctgactgga ttcaatggtc gatgaagctt gaatcagatc
     1141 aaaaaacaaa agaagatcga gaacgaaaac taattgtctt tcctgagaaa aaagaaattc
     1201 gtcaagtaga tgaaccgtgg ttcgatatgt tacttcataa agcaaacatc gtcggcggag
     1261 aaggacgtct tggtcgaaat aatgtgattt tcacgctttc tttagcttat tactcttcag
     1321 gttatgcatt ggatacttgc gaatacaata tgttcacatt caacgaacgg ttacaggaac
     1381 cgctatctga agcagaatta gtaaagattg tacgtagtgc ttattctggt aattatcaag
     1441 cagcaagcag agaattcatt attgaattgt gccagcaatg ggtttcacca accttaaaaa
     1501 ataatgatct gttcattcaa cgacgtggct ggtggaaatt taaaaagcca cgagatcaaa
     1561 gaaaatatag tcataaaaat gaatggaaag aagatctatt ggcttatcta agcgaaaaaa
     1621 gtcttgctag tgagccttat ttgaacctgt caaaaaaaga attggcttca caactaacga
     1681 tgcccaagcg aacacttgat aagatcttaa atgagttacg agaggagaat aaagtcttct
     1741 acaaggtaac acgaggaaga aatggcggaa tgatcattgc ttcagtcaaa acgttgttcg
     1801 cgagggttat tcaactaagt aaagaaaaga gactagatta ttcaacaagg atcacagagg
     1861 tatttggact caacgagagc ttagtagaag agacatttag acaattatca acccccttga
     1921 accctcttaa agaagtgaat ctttttgaag tagatacagg ttgaaaaata aaaggtgcaa
     1981 attgccttat acaatatatc tatgatactt ctgtaggtgt tgctcccttt gcctagctac
     2041 gtgtaggtgg tatacttata actgcgtatg cagtcttatg tatcatggag gaagttgtat
     2101 gtcgttaaaa aattatgtct attcacatcc tgttaatgtg tttatcattg gtcgtttggg
     2161 attatctgtg gaagtgttct gtgagcttta tggttttaag caggggactt tgtcttcttg
     2221 ggttaccagg gaaaaaacag ttgcttcttt gccgattgag tttcttcatg ggttgtcctt
     2281 agcttctggg gagactatgg atcaggtata tgattgtttg tgtgtgttag agcaggagta
     2341 tattgagtat aaaatagcta acgagttaag aaaaaggaag aagtatatcc agtagtagtt
     2401 tttatttact cttaattgtc aaattaagtt agacaaatca tcaatactta cgtaactcaa
     2461 aaatacttcc aaaggtgttc gataattcag tgattttcta ggaatattat ttcgtttcga
     2521 tgcgacagat tgaataaaag attcatctac tgaattgaaa tccatagatt tcaataaacc
     2581 atctctacgt aacaatccgt tagaattctc gtttaggcct ctttgagacg gcgttcctgg
     2641 atcagcgaaa taaatggcaa tatcattggc attactgatc tgtttccaat ttgaaaattc
     2701 ctttccacaa tcaaaagtga tggatttgaa tagatttttc ggtacagatt caaaccaatg
     2761 atttaatttg ttttcaatat caatcgcttg tctaccacac ggtttcaatg tgatgataac
     2821 ttttgataat cgctcaacta aggtaattac agcactttta tgtttcaaac ctacgatagt
     2881 gtcaccttca aggtgaccaa actcatttga gaattggcta taatcttttt cacgttcatg
     2941 aatagagcga cggaaagctt gttttccacg tctttcttga tgtccatttg gtttacgctt
     3001 gcctttcatc ggtaagtcag aaggattaaa tagccccttt ttgaacatac gataaatggt
     3061 acgagcagaa caacgaatag gaaacgctgc acgaccaaca atcacatcgg gtgtccatcc
     3121 ttgaacaacc tttctttgaa tatattctga ttgttcctca ggtaaaacaa gcgggcgtct
     3181 accacagttt gatttgtttt tcttatactg ttgatagtac tctaaggctg attttccttg
     3241 cttgaagaac aggtatactt tatggatcgt ttgtcttgaa cgattcaagc aatgcgcagt
     3301 tttcgcaaca gattgattta ttttgaaata agactctatc attacaagtt catccgttgt
     3361 aagatgggta taggtcattt gtactcactc cttataattc tttggacgga actattttga
     3421 gtgtagcaca aatgactttt ttagttgtct agcttaattt tacaatcggc gtacttaaaa
     3481 aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt taaaggaaag
     3541 aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc tggcttcctc
     3601 tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca ttaggaaaac
     3661 tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt ggagtatttg
     3721 gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac atttttacag
     3781 tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta gtcatgcaat
     3841 ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat ttaattgttg
     3901 taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga acaacaaaag
     3961 gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg tatttgtttg
     4021 aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt aaacgttcaa
     4081 ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag tacaatgttg
     4141 attttggaag atatgacgaa ttgat
//
LOCUS       DACDIP010000123         4117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDIP010000123 DACDIP010000000
VERSION     DACDIP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.126"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            168..1142
                     /locus_tag="IV257_002722"
     CDS             168..1142
                     /locus_tag="IV257_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8815782.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1124..1585
                     /locus_tag="IV257_002723"
     CDS             1124..1585
                     /locus_tag="IV257_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8815783.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1635..2099
                     /locus_tag="IV257_002724"
     CDS             1635..2099
                     /locus_tag="IV257_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8815784.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2113..2862
                     /locus_tag="IV257_002725"
     CDS             2113..2862
                     /locus_tag="IV257_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8815785.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2862..3692
                     /locus_tag="IV257_002726"
     CDS             2862..3692
                     /locus_tag="IV257_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8815786.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3692..>4117
                     /locus_tag="IV257_002727"
     CDS             3692..>4117
                     /locus_tag="IV257_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8815787.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPN"
BASE COUNT         1410 a          533 c          784 g         1390 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctggcatttt attatataaa attgttataa
       61 aaaaattttt ttgaaaaata gtattgcaaa ataaagcaat ttcatagata ataggtattg
      121 taaagttata tgactttata atacttatta gagtgaggga taaagaaatg agacaactac
      181 acaaagaaca gattgaaaca gcaatggcag cgttaaagga aaagacaatt gatcatgtat
      241 attttacagc atgtgggggt tcacaagccg ttttaatggc tggtcaatat ctatttgata
      301 aagaattaac tgtaccttgt catgtttata ctgctaacga atttaactat gctataccaa
      361 aaggattatc tgaaaattct ttagttatca cttgttccca ttctggcaca acaccagaaa
      421 ctgtagaagc aacaaaaaaa gcaagagaat ttggagcaat gactatttgt ttatctaatg
      481 aagaaggatc tcctctatgg gaagcagctg aatatcctgt tcactatgat tggggtaaag
      541 atgttgatgc tagtgattta aataaaggaa ttctttatgg tttattattt aatattttac
      601 ttgttttaga tggctctaaa aaatgggaaa cttgcttgaa tgaatttgat aagttaaatg
      661 atcttgttat tgctgaaaag agcaaatttg aaataactgc taaagaatgg ggtaaaaacc
      721 aaaaacgaga taaaattatt tacacaattg gtagcggtat caattacgga gaaatttact
      781 ccacagcagc ttgctggttc atggaaatgc aatggattaa ttctaatgct attcattctg
      841 gggaattttt ccatggtcct tttgaaatca ctgattatga tgtaccattc atcttagtga
      901 aaagtattgg agatacaaga tttttagatc aacgggtaga agacttcact aaaaaattca
      961 gtaataaatt aatggtttta gaccaagctg attttgactt agaatctata gatagtgtag
     1021 cgaaagaata tattgcagct gtacttagtg gcgttgttat tcgccaaatg gtagaagcga
     1081 ttgcttttga aagaggccat tccttagatg ttagacgcta tatgtggcaa atgaaatatt
     1141 aattaatagg aggttgcaaa agatggcaaa tcaattattt cacattgtga ttacaacaca
     1201 ttctaccctt tgtgagggtt atttaaaggc aacaaaatta attttaatga atgacgccac
     1261 aggtattagt gctgttccat ttaaagaaaa tactagtcca gatgattttg aggagagtat
     1321 gaaagaaata attagtcagc atcatttgca acctctaatt attttaacgg atttaattgg
     1381 aggaacacct aataatgttt ctattaaaca gttgaacaac aatagaattc aaattattgc
     1441 tgggattaat ttacctcttt tactggagtt attaatggcc cagtcagctg gtaaatctat
     1501 agaagaaata gattttaagg aattactgaa taactctcaa aaaagtatgg ttcatattaa
     1561 ttcgctttta aaagatgtca gttaaggtgg caatttctat tctataaatt ttaaaaattg
     1621 aaggaggaaa tagaatgatt aaattagtgc gtgtagacca cagattactt catggacaag
     1681 taatattttc ttggactaaa cagtatgata ttaaccatat tattgtggct gataacaaag
     1741 ttccaggaga tccaatgtct gtgatggctt tgacaattgc taaacctgct gattgcaaat
     1801 tagacattgt taaaattagt gatgttaaag ggattatgga aacatcccca aatgataatt
     1861 acatgatttt aattaaagga cctaaggaag cttttgaatt agttcaaaat ttaccagaaa
     1921 ttaaagaaat taattttggc ggtgtgtcta agaaaagcga gtccaaacaa tatggaaagg
     1981 cagtcttttt aactcctgaa gaattagaag ccacaaaaaa aattcttgat caagaagtga
     2041 aaatatttgt gcaacaagtc ccatcttcaa gtattgaaaa tgtagatttt agtaagtaag
     2101 gggggaaaat aaatgttatt aaaagccatt ttattaggtt tagttggtgt ggtagctgtt
     2161 attgactcca ggttaattgg tcgacaaaat attggtagac cactaatttt gagtacactt
     2221 gccggacttg tgctaggtga cttgcataca gggattgttc ttggggcatc tttagaatta
     2281 atttctatgg gattcgtctc aattggggcg gctggacctc ctaatatgca acttggtagt
     2341 attattgcca ctgcttttgc aattttaact aatagtagta cagaaacagc cttaacaata
     2401 gctatcccag tggctgtagc tggtgaattt atgagtatta tcatgcgcat gataattgct
     2461 caatttggac atgctgcaga taaagccatt gaaaataata aatttagaaa agctcaaatt
     2521 attcatattt attggtcatt tatttttaat gcttttgttt actttattcc aatcttttta
     2581 acagtatatt ttggtgctga cgttgtggca gatttagtag ataaaattcc agaaatgatt
     2641 acagacgcct tgactgtctc tggaaatatg ctttccgcat tagggtttgc aatgctatta
     2701 agcacaatgt tgagtaaaaa attatatcct tactttattt ttggattctt tattgttgcc
     2761 tattcaggtc tttcactaat tggcgtgaca atttttgcag ctctgattgc ctttgtaatg
     2821 gatcaagtta aatatggaaa aagagaagag gcgcatggtt aatggaaaat agtacagaaa
     2881 aaaaacgttt aatcacaaaa aaagatctta ataagatgtg tcttcgttca tggacaatca
     2941 tgtgttcgtg gaattatgaa agacaaatgc atatgggatt tatgtatggg atggcgccaa
     3001 ttttaaataa agtttataaa aatgatgaag agaaaaaaag agaagcttat actcgtcata
     3061 tggcattttt taactgtacg cctcaaatga cttcatttat tatgggactt tgtgcttcta
     3121 tggaagaaca aaatgctcaa gcagaagaag gaaactttaa tactgaatct atctccatga
     3181 ttaaaaccag tttaatgggt ccttttgctg gaattggtga ttcatttttc caaggtactt
     3241 taagaattat tacatttggg attggattgt cttttgctaa gcaaggtagt attttaggac
     3301 cgattttagc tgttggcctt tttgctatac cttctatttt agctgttttt tacggcggta
     3361 aattaggata tgagtctggc aataaatacc ttactaaatt gtatcaagaa ggtcttatgg
     3421 atcgagtaat gcaatttgct tctatcattg gtttggctgt agtaggtgga atggtggcta
     3481 gcatggtgcc aattgtcacc cctttaaaat tcactacagg gggaacagaa cttgttttac
     3541 aagacttact tgattccatt attcctggat tattaccact tgtatgtact ttgggaatat
     3601 ataaattgat taagaaaaat gttagtacaa atatgctctt attaggttgt attttggcag
     3661 ggtttgtttt aagtataata ggtatcttat aatgattaag gctctaattt ttgatatgga
     3721 cggagtttta attgatagcg agattatgta tcaaaagtgg gtatttaatt ttttaaaaca
     3781 agaaaattta agctatcctt tgtattctta tcaaaaaaag attggaacta cagcaagtat
     3841 ttttgatgat atagaaaagt acaatgaggg atgtaacagt ttagaattga agggaaaatt
     3901 tactaaatat tggaaatcgc aaaagattga ttacttggat atttttccta atatactaat
     3961 tgaacagctt gaatggttaa aaaagaaaaa aataaaaatg gcaattgctt cttccagtcc
     4021 aatggatact ataaatgaag ttgttaccac ttgtaaaata gagtcatatt ttgattgttt
     4081 aattagcgga agagatttgc cagaaagtaa acctaat
//
LOCUS       DACDIP010000124         4094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDIP010000124 DACDIP010000000
VERSION     DACDIP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.127"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            594..926
                     /locus_tag="IV257_002728"
     CDS             594..926
                     /locus_tag="IV257_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815788.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
     gene            1051..1308
                     /locus_tag="IV257_002729"
     CDS             1051..1308
                     /locus_tag="IV257_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8815789.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            1540..1944
                     /locus_tag="IV257_002730"
     CDS             1540..1944
                     /locus_tag="IV257_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP8815790.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            1963..>4094
                     /locus_tag="IV257_002731"
     CDS             1963..>4094
                     /locus_tag="IV257_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8815791.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLS"
BASE COUNT         1501 a          559 c          754 g         1280 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tacgttatgt agtaaatgta atagtttttt tggtagtgag tatgttgagg
      241 aatatattag atttgcagaa gacaataaaa atttccttta taacaataca agtttaaaga
      301 atggtaagtc tgatttaaca cattcaataa agaaaatgaa ttcacttaga gttgcaaaag
      361 caattgtagg aatgtttttt agtttaaatg gggatgaaga ttcaatggat aagccattct
      421 tagattcggt acgagaatat cttagcaatc ctaaaagtac tttatttcct catgaagact
      481 acaaaattat aatgaattat tatcttccaa taggtgctcc acagaattct aaaatcaaaa
      541 ttcctaataa cattgtaaat gatggtgaca ctcacaatgt aataaaaggt tttctgcccc
      601 ttgatctatc aactgggaga aaaattcgat tttcagaaat tcagtatgaa ggaattggtt
      661 ttactcttat agatttaaaa aattcaactt ttgaaataaa agaaggatta gatttaaaaa
      721 atctcctttg tgcagatgat aaaatcaaaa aaatacaatt gtacaaagtc cctatattaa
      781 caccaacagt acttacaatg ttggagatgg aaaataatat taattatatt gactcaggtt
      841 cagatttgct gattaggtgg caggtaataa ctaattttct tatggaatta aaaaaataca
      901 gtaatactca aacgaagttc tcttgaagtt ataaagaata caataaatat aaatttcctt
      961 tagactatat taaaatcagc aatcgaagga aaataaaaga agaaaatggt acgcaaaatc
     1021 taactattag agcaaaaaca ggaagctgct ttggaagata gtcccgaatt gtttccgatg
     1081 ttaaaagatc gtttaaatga attgactgaa aaacgaagac agttacaaat tcgagagaat
     1141 gaaatcttag ggatctttca acaacaaggg gaaccgattc aagtcaaaga tgtccaacag
     1201 atattgacta gtttggattg ctttcttatc cagagtgaga agaagcaagt taaagctttg
     1261 tatcacacct ttattgagaa aatcaccttt gatcctaatc agaagtagat attgccgatc
     1321 ccgatgagat tcacacctac tgtgatttga taattgaatg agtaatatca aatagccgta
     1381 tccaaaccaa aggatatggc tatttttgtg ttagggatat acgtcacgta gcttgttatc
     1441 aagaataagt ttctttattt ttttgctttt tctaaaactg agtatgctaa aataagaggg
     1501 tatatagcaa ctttaaataa gagataggag tgtatctttt tgggtgcaga attaaatcag
     1561 aagttattta gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga atataaaaat
     1621 tatttattgg ggctgatttt ctataaatat ttatccgacc gtttgttgga acaagtggtt
     1681 cttttagcag atgaatcctt agaggaatat gacacagtaa gcaaacaaac gatgttgtat
     1741 cgtgaattac tttcagatga agaatcaaaa gaagatctga ttgcaacaat cgttgatatt
     1801 ttggggtatg cgatttcgcc agagtatctt ttcaacgtct tagcggatca agcaaaacaa
     1861 gcaacgttcc aattgaatga tctaaacaaa gcctttttag caagtcatat ttatggaaaa
     1921 atactgtcgg caaaattcga atagtgtcga ggaggtgctg tgatggaaga attaaaagaa
     1981 cgcattgtca attatgtcat agagaactct gagattaaaa ataaatgggg gaacaataca
     2041 gcacagaaaa tatctgaaaa attaaatgtt agtcgtaata ttgtctctca atatttgaat
     2101 gaattcgtaa aagatggaga atttggaaaa atcaatactc gtccagtaat cttcttttct
     2161 ttaccggaga aagttccaaa tcaacaaatt tttagctctt cagaggaagc ggaaaaatgg
     2221 tatgatagct cttttagggt tggaatagaa agggttatcg gaagtcaagg aagtctgcga
     2281 aatgtaattg ctcaaattaa agcagcaatc tactatccgc caaatggctt acccatttta
     2341 cttcatggcg agactggaac tggaaaaagc tatttagcaa attatgtgta taaggatttg
     2401 ttggccaata attatataga agagaaggca tctttcattt cagttaattg tagtgaatat
     2461 gccaataatc cagaactttt cttgtccaat ttttttgggc atgtaaaagg tgcatataca
     2521 ggagccgaag atggaagaaa gggtttagca gaattagcta ataatggagt cttattttta
     2581 gatgaaattc atgcattgag caatgaatgt caggagaaac tttttcagtt tatggatacg
     2641 aggaaatttc atcggatggg tgataatgaa aactggtaca catcaagttg ctggattata
     2701 tttgcaacat cagaaccacc tgaggataaa ctattatcaa ctttgttgag aagaatacct
     2761 gtaaagctaa cacttccaac attagacagt cgttctcttt atgaaagaaa acagttaatc
     2821 aagatatttt ttaaatcaga agaagaaaga ctagcaaaga aaatactgat atctcaacaa
     2881 ttattagatt ttttattaaa ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc
     2941 attcaattga cctgtgccaa tgccttttat ttcttagatg atttggaaga actttatatt
     3001 cagatgtatc atttaccaca atgggtgaat ttcaacggca tgaaggttat gaaaagacag
     3061 aatgaattaa aatatattca aatggatgat ttggaaaaag tagacgaagt ttttaatatc
     3121 aacgaactta tagagtatct ttatcaaacg tggaagaaag acactgataa gcagcagctt
     3181 tttgagaaat atgatagata ttttagacaa aagaatgttg atatccaaaa taattcagct
     3241 ttaacatatc ccacctatca agagattcga tttagacagt atgttaagga aatagagaga
     3301 gattttcaaa tagaatttga ctattattat cttcaaataa cgtctatctt tttgaattct
     3361 tggatttatc aaatccaatt aacatcggac gataaattat attccttcat caatgatatt
     3421 aatagttggt gtagtcgaga agttcaatta gtcaatcgtt ttattcaaat aggggatatt
     3481 tatttaacaa aaattcaaaa aaatgttttg gttattttat ttaagttaat gtgtagtaaa
     3541 gaatttcagc aaaaacgttt agcactgatt ttggctcatg gagatctaac ggcaacctgt
     3601 atagcaagta ctgctaatag gatcgttgag agatatatat acgacgccat tgatatgcca
     3661 ctaatgactt caagtagtga gattgcagac aaaattaata gatacttaca agagtgtacc
     3721 ggatatgaag aaattatttt acttgttgat atgggttcac tggagaaaat ttatctaaat
     3781 ttggaactag acgctcaaaa aaaattcgga ctactaaaca atgtttcaac aagaatggct
     3841 attgatgttg caaatcagat attattagat ttaccgctta aacagattct tgaacaagta
     3901 aaagaaaatc aaaatgtgga ttatatttat gaatcatcaa atattaagaa ggatatgatt
     3961 gtctgttctt gtgcaagcgg attaggaact tcgtttaaat taaaaaggat acttgaagat
     4021 agctttccaa gggatactga tcttgtcata gaaacgtgtg actattactc tttagtgaat
     4081 gaaaactatt tatc
//
LOCUS       DACDIP010000125         4004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDIP010000125 DACDIP010000000
VERSION     DACDIP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.128"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..904
                     /locus_tag="IV257_002732"
     CDS             47..904
                     /locus_tag="IV257_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8815792.1"
                     /translation="MIFFFYIPVMANVVAFKNFHISADGFLASLRESPWVGWENFKFL
                     FSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLS
                     WVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSI
                     IYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGL
                     FYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIAR
                     RFDSDSALF"
     gene            916..1836
                     /locus_tag="IV257_002733"
     CDS             916..1836
                     /locus_tag="IV257_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8815793.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1877..3334
                     /locus_tag="IV257_002734"
     CDS             1877..3334
                     /locus_tag="IV257_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8815794.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3679..>4004
                     /locus_tag="IV257_002735"
     CDS             3679..>4004
                     /locus_tag="IV257_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8815795.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFI"
BASE COUNT         1266 a          678 c          808 g         1252 t
ORIGIN      
        1 gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga tttttttctt
       61 ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt cagcagatgg
      121 gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca aatttttatt
      181 ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg tagcatttat
      241 ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt tgagaaataa
      301 acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat cttgggtcat
      361 tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca atcagtggat
      421 cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt ggccgtttat
      481 cttggtgttc attggtacat ggaaagggat tggttataac agcattattt actttgcatc
      541 tgttatggga atcgatccga cttattatga agctgctatg gttgatggag ctagcaaatg
      601 gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga ttttaacgat
      661 tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca ttcctaggaa
      721 ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca atggcctgac
      781 atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag ttgtcggctt
      841 tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt ctgcattatt
      901 ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg ctctttcaat
      961 aagacggcta attttttctc taatataatg attgcactct ttgctatttc ctgtattttg
     1021 ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc gactcacggg
     1081 tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt cgaccagatg
     1141 caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt aggtacactg
     1201 atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa ctttccattc
     1261 cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg gatggtggcc
     1321 aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc attgatcttg
     1381 ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa acgttcggtc
     1441 tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg aatatttgtt
     1501 cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt tgccgcattg
     1561 ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa gctagttcca
     1621 ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca aaattcagga
     1681 gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc cagaatggcg
     1741 atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca gaagcatttt
     1801 gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat aaatcataaa
     1861 attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg tttcagcgtt
     1921 gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg cagaagctag
     1981 caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt tcgatcaatt
     2041 gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca atatcaatta
     2101 cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg gggaaaatta
     2161 tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct ttgctgattt
     2221 gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg aagcatatat
     2281 taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa atgtctttgc
     2341 tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt caattgatgg
     2401 aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa aagaaccaaa
     2461 cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg attatccttt
     2521 agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa tcatcaacca
     2581 atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt acaaacaagg
     2641 actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag gaaatacttg
     2701 gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt tgacaaatgc
     2761 agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta cagctcaagc
     2821 acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat cagtagaact
     2881 tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg ggatcgaggg
     2941 agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag atggttatca
     3001 gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat atacacaaga
     3061 gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg cagaaacttc
     3121 accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa gtaatatttc
     3181 taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc cagatgaaac
     3241 attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat tgaaagaaat
     3301 gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa aggggttgtg
     3361 acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc cagtttctcg
     3421 gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt tttcttacta
     3481 ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc agctaaccct
     3541 cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg tttctctaat
     3601 aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag tataaactta
     3661 atgtttggag gagaagatat gttagggaag gttttggaag agttatttat ccggatctgg
     3721 gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg gatcatcttt
     3781 ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg attcgagtat
     3841 aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt aagaggcaat
     3901 ctgctattta gtttattttt atctggtact gctttattga gttacaattt atatttatct
     3961 gttcaaatca aagggctcat ttttttagct attgatttta tttt
//
LOCUS       DACDIP010000126         3924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDIP010000126 DACDIP010000000
VERSION     DACDIP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.129"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            91..303
                     /locus_tag="IV257_002736"
     CDS             91..303
                     /locus_tag="IV257_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815796.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            417..2474
                     /locus_tag="IV257_002737"
     CDS             417..2474
                     /locus_tag="IV257_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8815797.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2512..>3924)
                     /locus_tag="IV257_002738"
     CDS             complement(2512..>3924)
                     /locus_tag="IV257_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8815798.1"
                     /translation="RNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKV
                     VGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALL
                     WTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSF
                     KITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFL
                     ANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNK
                     KVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDP
                     VEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQ
                     LIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTV
                     IGSILFLTLASLGYVVLKLK"
BASE COUNT         1293 a          726 c          676 g         1229 t
ORIGIN      
        1 tccacctttc tatagaaaat ttttctcttg atattgtgat tcattacaaa aaagagctat
       61 aataaagaaa aaacaagaag gagcattgaa atgtacgctg ataaaaagaa aatcgaatgg
      121 ttgctgtttg atagtggtaa atcagttcgt tctatatcca tagaatctgg cgtaccctta
      181 actacagtaa gtgaccttgc caacaaacgc tctagtatag acaacatgag attcataaat
      241 gctatgaaat taaataaatt ggctgaagaa atcgttgata ttaatacttc aactaacaaa
      301 taaaataaga aaagccaaaa ttatataagg cttttcttat tttttattga ctatatcggt
      361 tagccgatat ataataatca tgtaaacatt tttaattaaa tttaggagtt gaaaaaatga
      421 aattatttaa tccaaaaact gttttagcta ttggaacatt tgctgcctca cttagtcttg
      481 ctggatctgt tgacgctgct tcaactaacg tcatgtatcg cctttacaat ccaaataata
      541 atgaacattt ttacacctct agcaaaaaag aacgtgacca tctagtaaag ataaaatggg
      601 gaaattatga aggccccgcc tgggaagcac cgactagcgg tggccatctt gtctatcgtt
      661 tgtacaataa aggtcttcgt gatcaccatt acacagctag ttgggatgaa gtgaaatggt
      721 tgactaaaaa ttacggctgg acttatgaag ggcctgcctg gcgttctgcc gaaaaaaata
      781 ataaaccgat ctaccgcctt ttccttcctg gtgtaaaatc tggctcacat cactacactg
      841 caagttggaa agaagtacaa tggttaacca aagactatgg ctggaaatac gaaggtatcg
      901 gttggtatgg cgctgacacc tctaaaccca aagtgaataa atcggctcta gaagctcttt
      961 atacgagcgt taagggaacg aaaaaaggtg actataccga caatacctgg aacgcattcc
     1021 aaaccgcttt aaacaatgct aagaaagtgt taaatgactc aaaagcgact caaaagcaag
     1081 tggatagtgc caaaaataaa ttagattctg cttacaaagg attaaagaaa aaaccagttc
     1141 ctactgtgaa taaagctgaa ttacaagctt tatacaaccg agtaaaatca actgctaaag
     1201 gagattttac agaagaatct tggaataatt ttcagactgc tttatccaat gctaaaaaag
     1261 tactagacga ttctaaggtt tctcaaacac aagtaaacaa tgctaaaaat agtcttgatg
     1321 ctgcatttaa agggttgcaa cacaaacctg aaacaccaaa agaatatacg gttactatta
     1381 accatattga caatcaaaca ggtcaagtga ttgctacaga tcaagcaaaa gtaacttctg
     1441 gttctactta cactgctaag gcaaaagcat ttaaatatag cgaaggcgct aaagataatt
     1501 ttagctacaa agtgattggt aatgatactc aaagcaaaac aatcacaggt aatacaacaa
     1561 tcacattcaa ttataatcaa gtgcatcaaa tcaatttgtt gtgcaataac aactacaaaa
     1621 acgaaggacg agttaatatt ttgaaagaac agatagtcaa tgtcactcac ggtgacagtg
     1681 tcactttaac tgctccttct attcaaggat atgttttaga cgatcgggta gaaccaacaa
     1741 attcagtaac cttaaacaat gtgactgaaa gtcaaaatta tattttcaac tacactcgta
     1801 aatttaatgt gactgttaat catgtaaatg ctgataccaa cgctgttctc tctagtgaat
     1861 caaaaacagt atacgaaggg gatagctttg ctactccttg gaaaaacatg actgatcaaa
     1921 attatttctt gtgcagaaat gacgatccta ccgttaccgt ggataaaaac ggccaacgat
     1981 ccattaataa cgtcgatcaa aataaaacaa tcacattcaa atataaaaag atttcattag
     2041 acgaattacg taactttatg cgtcaaaaag aacttgcttg gttaaatgat tatcgccaac
     2101 aaaatggtgt tgccccaatg caattcaatg atattgttca acaggctgca gatattcgag
     2161 ctaaagaatt acaagtcagc ttcagtcact atcgccctgg tggtggtact ttccaagatt
     2221 tattggaaag cttaggctgc tacggtgcta agggagaaaa cataagtcaa actgggctgt
     2281 atattgatga ccttttatca gacgatggtg ctatcgatgc catggtagga tggaaaaata
     2341 gccctgccca taatgcaaat ttactttata ataaccaaag tattctcgga ttaggtcata
     2401 attttgtagt tgactctgct ggtagaattg gcgtagataa tgtatttatt agtgcgaatc
     2461 caatcttcaa ataaaagaaa acaaggctga ttctcagcct tgtttttata tttatttgag
     2521 ttttaaaact acatagccca atgatgctaa agtaagaaat aatatacttc caataacagt
     2581 tagcactaaa tgattcgatc cagtttttgg cacaatgatc tgaggtaagt taggttttac
     2641 cccaatcgtt actacattag ttgctttctc aatactttct actgaaactt ttgcttggtt
     2701 gtcgatatcc aattcttcat tcaaaggagt tttagctgta aatatcagct gaatttctcg
     2761 tccatctaaa agatttttac ttaagaaatc agcggaagct tcccacttca gcgttcttga
     2821 attgctatca taggttagtg taccttctga agtaatatct tttcctgtac tcatatctat
     2881 cactcgcgca gaaccttctt tatacgtcaa acgatcttcc actggatctt gaatactaaa
     2941 ttgcttgtaa tgatctgagg caatgtctac accttttgta tgccattttt gtgtgactag
     3001 atattgtact tcagatcctg catttaaagg agctgtaggc gttccttttt taccatcatt
     3061 tcgaccatca acatctttcc catcttgttc cactttttta tttggtttaa gagagaaata
     3121 gttttctact gtatttgttt tttgtgttcc tttgttcgga tcattgttaa acgttacagt
     3181 agccacattt ggtgcaattc cttttgcatc attttttgct ctcatatgga gctgcaaaat
     3241 ataatcccta ccgtaatttg cagcgttagc taaaaagctg tcagaagcag tataagtgac
     3301 tgtattacct gatacagtta actttcctcc tgtatcagct tttttccaag taccattcgc
     3361 ttcaacatac cacaatgcgc catcatagtt tttcccatcc gataccatat caaaattttt
     3421 gctgtcaaag gtatcagtga ttttaaatga tttgtatttg ttcattgtgt tgactgttaa
     3481 tgcatcaaac ttttggtgaa tatgatagta aacatctgat ccttgtggaa tcactttttt
     3541 gtcaatattg tgtgctgcct cttcagtatt agctttgtta aacacttctt ttgtaggctt
     3601 cgatggttgt ggtgtccata gcaatgcagt atcaaagcta aacatcatac tattcgtatt
     3661 tgcgccaatc atgaatgttg gggaagcgcc ttgaagcgta tacgttacac cacttctgtg
     3721 ataagtatca gctcctacaa catcggtgaa atgatcattc gagctataag ccttttctgc
     3781 acgtcctccc actacttttg aagttaaatt gtttgttgat aacgcaggag ctttgtattc
     3841 tgcacatgct gtatctgttg tgacaaaata aggtaagcta cccatttttt catacccaac
     3901 aaattctctc atatcaatat tacg
//
LOCUS       DACDIP010000127         3746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDIP010000127 DACDIP010000000
VERSION     DACDIP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.130"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(351..2123)
                     /locus_tag="IV257_002739"
     CDS             complement(351..2123)
                     /locus_tag="IV257_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815799.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSRTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(2077..>3746)
                     /locus_tag="IV257_002740"
     CDS             complement(2077..>3746)
                     /locus_tag="IV257_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815800.1"
                     /translation="VLTVYGYYSIYKFLKSLIIVGDEYLAKSYAVQTVAWLTLGALFY
                     IFSGLFSHILGFRLETNLRKKGISGLTEASFRFFDLNSSGYIRKTIDDNAAKTHMAIA
                     HLIPDSAQAIVTPVLSVALGFFINFKIGLVLIAMLVIGVFLLKKMTENTNFIQKYQES
                     LDVLSSETVEYIRGIQVIKIFGAKVTSFKALNKAINDYAKFAYKYSLSGKTPFVLFQW
                     LFFGIVCILIVPITFFITSLTTPKILAVDLLMLFFLSGIIFVSFMRIMYVSMHLFEAR
                     YAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFENVSFSYKEECVLENFNLVLEQKRI
                     YALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYSKDTIINTIAFVFQDTKLF
                     NQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPEREHTLIGSKGVYLSGGEKQR
                     IAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLMKEKTVIMIAHRLSSIKSVD
                     EILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWRLDNEKFL"
BASE COUNT         1255 a          689 c          515 g         1287 t
ORIGIN      
        1 tcaaatcatt taaaagaata tgcagtttaa gctctgaaaa ataagaattc acttttatta
       61 gtccaatcaa acacaaatac cgttttaagt agcttttcct ctacttaaaa cggcaatatg
      121 aaagaaacta taacttttta ttcaatcttt tatgtttaac tggtttatcg ccaatttttt
      181 aaaactttga ttattcctta tattataaga atagacatta agtgaactaa ttcaatcata
      241 ctgattcttt cgtgtattca aaattagatg ctgtttaata aaattattct ttttgaaaaa
      301 gaattataac tatatcccaa aaattgtagg ttaatttaaa gcctatttca tcatcgtgat
      361 tgagcttctt tttcatagac gaatttctca gatagtttag ttttctttat gagatttttg
      421 tatatttctg accttcgata caactcacta tgagtacctt gatcaactat ttttccttga
      481 tccatcacta caattttatt taccttttca atagacttca agcgatgcga tataattagt
      541 actgtcttat cttgtattaa acgatttaaa ctttcctgaa tacgtttttc attttccgca
      601 tctaaactgg cagtaatttc atctaaaatt aaaattggcg catttttcaa aaaagcccgt
      661 gctatggata ggcgttgtcg ctctcctcca gaaagtgaac ttccgttttc tccgaccaat
      721 gtatgataac cgtcaggtag tttttcaata aagtcttcgc agttagctaa acgagctgct
      781 tttttcactt cctcatctga cgcttgacta tttccaatac gtatattttc caaaatactt
      841 gtattaaaca aagttacctc ttgaaaaaca atagcgattt tgctaaacaa agaagctgga
      901 gatacacgtt taatatcata cccatcaatt aaaatacagc cttcatcata atcatacagt
      961 ctagacacta atttcaagat actagttttc ccggaaccac tagcacctac taacgcagtc
     1021 acttctcctt gtttagccgt aaaagagata tgatctaaaa ttggggtatt gttatcatat
     1081 gagaaagaga catctcgcaa ctcaatgtca aaagatttca gaggactatc tgatccctct
     1141 tggatactgg tttccttcat agctctaatt cgctgtattt taggagctaa ataaaatatt
     1201 tcaagcactg cttctttcat agaatcgaag gaatccttta tttttattgc ggctagtaag
     1261 taaccaacga cgtagagtat agtcacttca cccgttaaca gtaagtgaac acctactaga
     1321 atgactactg ctaaactaac atatgagaaa atagaggata aggccatgac tgaaaaagta
     1381 cttaattcta cctttaaatg aatcctttca ctctcttcca tttttttgaa aagtctgtct
     1441 tgaacttttt tagatagatt aaagctatta atctcttgct gcaattctat agtttcttga
     1501 aattcttccg agttttctct caaagtatcg taatatttag tattggcttt cgtttgggat
     1561 ttctttgata acaagattaa gacaaaacta aataacgttg gcaaaataac agctaatccc
     1621 attttgacat taccaaccaa aagcatcact gaaataaaag ggaaaaacag agccataccg
     1681 ccggctttag gtattgcatg actcatcgca tgctcaatac cttcaacgtc agacatgata
     1741 gttcgagata aatctgataa attatgtttt gaaaaatagg ataatggtaa atttgacaaa
     1801 tcgtctgcaa tttgcattct taaatgcgca ctttctttat aagtactatt atataagttt
     1861 tcatactccc tatctaacaa ccaatacatg aaaagcaaag taacgaacga tactgtaaaa
     1921 taaagccaag gcggctttac attttctaaa actaattcgt ctatcagcat cattataatg
     1981 aatgctggta ccatattaat gcagtataca aaaaagctag aaatagatgc tttaaccaaa
     2041 tcttctgcgc cctgattagt taaagcgaat ttgtttttat aaaaactttt cattgtcaag
     2101 cctccaatca tttgcattct cataattagt gaccaattgt ttatacactt gatgtttagc
     2161 cattaattca ttatgatttc cacgttctac tattttccct tctgacaaga caagaatttc
     2221 atctacagat ttaatgctgg ataatcgatg agctatcata ataactgttt tttctttcat
     2281 cagatgttga aaagcctttt gtagttcata ctcattatct ggatcaatag atgcactggc
     2341 ttcatccata ataacaatct ttgcatcttt taaaattgct ctggcaatag ctatacgttg
     2401 tttttctcct cctgacagat aaactccttt actaccaatt aaggtatgct ctctttcggg
     2461 aaatttttct aaaatcgatt cgcaaccggc taatcttagc gcgtcaaata ctttacttgc
     2521 tggcgcttga ggatttgcta tagaaacgtt atcgtaaata ctctgattga atagtttcgt
     2581 atcttgaaag acaaaagcaa ttgtgttaat aattgtgtct tttgaatagt gatttaaagg
     2641 cttaccacca attttaatct gtcccttatc cacattgtag aaacctgaaa ttagtttagc
     2701 tatcgtactc tttccgcttc ctgattttcc tactaatgca taaattcttt tttgttctaa
     2761 aactaagttg aaattttcta aaacacactc ttctttgtat gaaaagctga cattttcaaa
     2821 tgcaatgtca caatttttaa tctgtgtctc gttcccatat tttagttggt cttgcttcat
     2881 ttctaaataa agggagtcta gtgtttcgac tgcatatctt gcttcaaaca agtgcatact
     2941 tacatacatt attctcatga aagaaacaaa aataattcca cttaaaaaaa acaacatcaa
     3001 caagtcaaca gctaatatct tgggagtggt aagagaagtt ataaaaaagg ttataggaac
     3061 tatcaaaatg caaactatgc caaaaaatag ccattgaaaa agaacaaaag gtgttttccc
     3121 actcaaagaa tacttatatg caaacttagc ataatcgtta attgctttat tcaaggcttt
     3181 aaatgatgtt actttagcac caaatatctt tataacttga attcctcgga tatattctac
     3241 cgtttcagag cttaacacat ctaacgattc ttgatatttt tgaataaaat ttgtattctc
     3301 tgtcattttc tttaacaaga atacaccaat tactaacatg gcgattagaa ccaatccaat
     3361 tttaaaatta atgaaaaagc ctaaggcaac tgataaaaca ggtgttacta tggcttgagc
     3421 actatctgga atcaagtgag caattgccat atgtgtttta gccgcgttgt catcaattgt
     3481 ctttcgaata tatcccgaag aatttaaatc aaaaaaacga aaacttgctt ctgttaaacc
     3541 gctaattccc ttttttctca aattagtctc taagcgaaac ccaaggatat gagaaaataa
     3601 gccagaaaaa atataaaata aagctcctaa agttaaccaa gcaacagttt gaaccgcata
     3661 actcttagct aaatattcat ctcctacaat aattagagac ttaagaaatt tgtaaatact
     3721 gtaatatcca tagactgtta atacga
//
LOCUS       DACDIP010000128         3733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDIP010000128 DACDIP010000000
VERSION     DACDIP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.131"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(267..1565)
                     /gene="eno"
                     /locus_tag="IV257_002741"
     CDS             complement(267..1565)
                     /gene="eno"
                     /locus_tag="IV257_002741"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8815801.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1681..2436)
                     /locus_tag="IV257_002742"
     CDS             complement(1681..2436)
                     /locus_tag="IV257_002742"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8815802.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2540..3730)
                     /locus_tag="IV257_002743"
     CDS             complement(2540..3730)
                     /locus_tag="IV257_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8815803.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
BASE COUNT          944 a          782 c          700 g         1307 t
ORIGIN      
        1 atttatacga gaggacttgc ccatagattc ctattctact gttctatcct taaaagctct
       61 tatatgatgc tagttattgt tagctttcct ttttcaagga tgatcttttc ttgtaccatt
      121 ctttgtaaac atcatataca gaagctagtt gcaaaaaaac tctctacctg ctgatatttc
      181 agcaagtaga gagttcgatt acttaactaa gtgatcatac tcgactatct ttcaatagtc
      241 gatcatgtat cttcattaga aaagaattat ttgtttttta agttgtagaa agatttcaaa
      301 cctttgtatt ctgcaacttc accaagttgg tcttcgatac gtagtaattg gttgtattta
      361 gcaatacggt cagtacgtga tagagaacca gttttgattt ggccagcgtt tgttgcaaca
      421 gcgatatcag agattgttga atcttctgtt tcaccagaac ggtgagatac aactgctgtg
      481 tagccagctt ctttagccat ttcgatagct tcgaaagttt ctgttaatgt accgatttgg
      541 ttcactttga taaggattga gttagcaatt cctttttcga taccttcaga taatttttga
      601 gtgtttgtta cgaacaagtc gtcaccaact aattgaactt tgtcgcctaa tacgtcagtt
      661 aatttcttga atccgtccca gtcgttttcg tctaatccat cttcgattga gatgattgga
      721 tatttagaaa ctaattcttc gtagaatttg atcatttcgt cagttgtttt ttcgccttcg
      781 cctgagtcag ctagtacgta aacacctttt tctttgtcgt agaattctga agaagcagca
      841 tccatagcaa gaacaacgtc tttaccaggt acatagccag ctttttcgat tgcttcgatg
      901 attacttcaa aaccttcttc gtttgaacca aggtttggag caaatccacc ttcgtcacca
      961 acagaagttg ctaatccgcg agctttcaag attgaagcta atgcgtggaa tacttcagca
     1021 cccatacgta gagcttcttt gaatgttgga gcgccaacag gcatgatcat gaattcttgg
     1081 aagtcgattg agttatcagc atgtgatccg ccgttgatga tgttcatcat tggtgttggc
     1141 aatacttttg tgttgaatcc gcctaggtag tggtataaag gtacttctag gtagtcagca
     1201 gctgcacgtg caacagcgat agaaacacct aaaatagcgt tcgcacctaa tttacctttg
     1261 ttaggagttc cgtctaaagc gatcatagct ttgtcgatag ccatttggtc acgaacatcg
     1321 tagccaatga ttgcttcagc aatgatgttg tttacgttgt cgacagcttt agttacacct
     1381 ttaccgccat aacgagcttt gtctccgtca cgtaattcaa ctgcttcgta ttcaccagtt
     1441 gaagcacctg aaggaaccat tccgcggcca aaagctccgc tttctgtgta tacttctact
     1501 tcgattgttg ggttaccacg tgagtctaag atctcgcgag cgtaaacatc agtaataatt
     1561 gacatgtttt gtctctcctt tgagtttgtt ttttcctaag ggatttccct catatacgat
     1621 tttagttaat tttgctcatt agtgcaaaca tttcttgcat ttttcgcaaa ttttcaacaa
     1681 ttattttaca gcgtctaaca atgataagaa tgaatctgct tgcaagcttg cgccaccgac
     1741 taaagcaccg tcaacatttt cttttgccat gtattcagca atattttctg gttttacaga
     1801 accgccgtat tggatacgaa ctgcttctga tacttctttt ccataaagtt tttcaactgt
     1861 ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg tcagctgatt taccagtacc
     1921 gattgcccag attggttcat aagcaataac catgtttgac acctgttcag ctgaaagatc
     1981 agctaaacca gctgtaattt gtccttcgat ccattcagca gttttgcctg cttcataggt
     2041 ttctaaagat tcgccgcagc ataggattgg tgtcatacca tttgcaaaga tagctttcgc
     2101 ttttttattg atgtcttcgt ctgtttcatg gaaatattca cgacgttctg aatgtccgat
     2161 gatcacataa tgaacaccta gatcagcaag agctgctggt gaagtttcac cagtgaacgc
     2221 acctgagttt tcccagtagc agttttgtgc tgagattttt agttctgtac cttcagctga
     2281 tgtcattaat tcttgtaaga ataatgcagg agatccgatg actgaatcaa ctttgtcatt
     2341 tgaagggatt ttgtttttta ctgcttcagc aaattctttt gcttctgatg cagttttgtt
     2401 cattttccag ttacctgcga taataggttt acgcattgag gaacacactc cttaatttat
     2461 tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt ttgttcgatt actcgtcaga
     2521 attgagcaaa cttggtcgtt tatttatcgt tgattgctgc tagtcctggt aattctttac
     2581 cttcaagcaa ttccaagctt gcgccgccac ctgttgagat gtgtgtgaat ttgtcagcaa
     2641 atcctaattg ttctgctgct gcggctgagt ctccgccacc gatgattgtt gttgcgcctt
     2701 ctaagttagc gattgcttca caaacaccga ttgtaccttt agcaaagtta ctcatttcaa
     2761 atacacccat aggtccgttc caaacaactg tttttgcacc ttgtaatgta tcagcaaata
     2821 attttactga tgctgggccg atgtctaaag ccatcaaacc atcaggaatg tctccttcga
     2881 ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca gacattgtcg attggcaaga
     2941 caagtttgtc gcctgctttt tcgattaatt cttttgctag ttccacttta tcagcttcta
     3001 ctagagagtt accgatcttg attccttttg cttcgtagaa tgtgtaagtc attccgccac
     3061 cgatcaagat tttatctgct ttaggaatca agttttcgat tacgccgatt ttatcagaaa
     3121 cttttgcgcc accaaggatt gcaatcattg gtcgttttgg ttcttctact gcttcaccga
     3181 tgaacttgat ttctttttcc atcaagaatc cagcaactgt tgggatacca gttgaagcga
     3241 tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa agcatcgtta acaaatacat
     3301 cgcctaatga agcccagtat ttacctagtt ctgtatcatt tttgctttct tttttaccgt
     3361 caatatcttc aaatcgtgta ttttcgaata cgacaacgtc gccgtcattc atgttgcgga
     3421 ttgcttcttc taattgttct ccgcgagttt caggtacgaa tgttacttct ttacctaata
     3481 attcgcctag acgttttgca actggagcta aagattttcc ttctttatct tcttctgttt
     3541 ttacacgtcc aaggtgagag aaaaggattg cttttccgcc ttgttctaga acgtagttga
     3601 ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat cacgccgtct ttcaaaggaa
     3661 cgttgaagtc aacacggaca agaacttttt tgtcttttaa atcgatatct ttcactgttt
     3721 tcttagccat ttt
//
LOCUS       DACDIP010000129         3680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDIP010000129 DACDIP010000000
VERSION     DACDIP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.132"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1616
                     /locus_tag="IV257_002744"
     CDS             <1..1616
                     /locus_tag="IV257_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8815804.1"
                     /translation="ETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAA
                     LSLGTEIVVPNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKIL
                     SVSPDENKGKDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLT
                     GIMLFSQILFIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAF
                     SISTMFFNISATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKP
                     QMLMNRTLRQLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHE
                     IPSDLNKEKPIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYN
                     FHKGIEKTKKAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKR
                     RENVPIKAKVQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVK
                     RRSTLSKRTNRKVQKSLNPHPKPRSGEKK"
     gene            1613..2617
                     /locus_tag="IV257_002745"
     CDS             1613..2617
                     /locus_tag="IV257_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8815805.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            2636..3547
                     /locus_tag="IV257_002746"
     CDS             2636..3547
                     /locus_tag="IV257_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8815806.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
BASE COUNT         1256 a          623 c          808 g          993 t
ORIGIN      
        1 gagaaactac caaagcagtc cgggctgtca ttaattttgt gatgattttt ctactttcgg
       61 gttcgttcat tgcgtatgcg cccacctata tcacaaaaat caatgatttt agctcagatg
      121 tcagcgaagc ggctcttagc cttggaaccg agattgtcgt gccgaattca gaaagccaag
      181 gaaaggacag tgtggattta atccgagata gcttgttttc cattcaagtc caacaaccgt
      241 ggttactctt gcaatttgat gattccaata tagaagaagt tggcgaagat cgtgttaaca
      301 aaatcctgtc tgtaagtcca gatgaaaata aaggcaagga tcgggaagaa gcggtcaaag
      361 cggagattga agataatgac aatgcgaatc tcagtattac caagaccatg aaccgcttag
      421 ggaccgtggt ctttttggtg ttgttcaata ttggcatatc cttctttgtc tttctcctaa
      481 cagggattat gttgttctcg caaattctct ttattatctt tgcgatgttt ttgccgatta
      541 gtttcttatt gtccatgttg ccgacttatg agagtttggg caagaaggcg attattcgct
      601 tgttcaatac gattatgatg cgagcaggag ttactttggt gattacgact gcatttagca
      661 tctccacgat gtttttcaat atttcggcaa cctatccatt tttcatggtt gcttttctgc
      721 aaattgtgac ctttgccggt atctatttca aattgggcga cattatgaat atgttcaatc
      781 tccagagtaa tgatagccaa tcaatgggaa gacgggtcat gcgaaaaccg caaatgttaa
      841 tgaatcgaac attgcggcaa ctcaatcgaa atgtgggacg gactttggct tttggtggtg
      901 gtgcagcagt tggaaataag ttggcaaagg aacaatcaaa atccaaattt aagccatccg
      961 gttcttctct acgaaagaat tcacgattac cgaacgatca cgagatacca tctgacttga
     1021 ataaggaaaa gccaattcca aataataaga aacaatcccg aatgaatttg gccgggagaa
     1081 aagtcggcaa ggttctggat acccaagctt tggtgaaaga taagaccaaa caagtaaagg
     1141 atggagtacg gaatacgcca accaacttga aatacaactt ccataaagga attgaaaaaa
     1201 cgaaaaaagc accggaagag tttaagcgag gactggtcca agaaaaagca aatcgaggag
     1261 agttgcgaga aaaacaacaa caacgtagag acgaaaaaat agctgaaaag cgcaaagtat
     1321 tgaacgaaat agggaatcct catgggaaaa gaagggaaaa tgttccgata aaagcaaagg
     1381 tccaatctca aaaggataga gcaccaaagc ggattgtgag agcaaattcg aatcccgaaa
     1441 tcaaacgaaa attcgctagc caagaaatca tttcaaaagg aataaaccaa tcctcaagta
     1501 agaaaaattc ttttcaacaa gtgaaacggc ggtctactct gtccaaacga accaatcgaa
     1561 aagtacaaaa aagtttgaac cctcatccca aaccaaggtc aggtgagaaa aaatgaacgt
     1621 aaagaaatta agtgccattt cgttaacttt cctaacaatc tcttttatgg gtatcttgat
     1681 gtgtgtgggc ttattgtttg gtgaagacag tgaaggaagt agtagttcag gtgctcctcc
     1741 tggggggagt agtgtctcag aagaggtttt gaaacaccgt gcaatggtgg aaaaatatgc
     1801 caaagaatcg gatataacag agtatgttcc gattctttta gcgattattc aagtggaatc
     1861 tggtggcaca atggaagatg tgatgcaaag ttctgagtca gccggtttac caccgaattc
     1921 attaagtacc gaggcatcca tcaaacaagg atgtctctat tttgcgtctt tggttaagcg
     1981 ttccaaagaa ttaggttgtg atcaagatag tatcattcag gcctataact ttggcggtgg
     2041 ttatttggac tatgtggcaa aaaacgggaa gaagcatagt tttgctttag ctgaatcttt
     2101 ttcaaaggaa aaatcttgcg gtcaaaaaat cgattaccct aatcccatcg cgaataagga
     2161 aaatggtgga tggcgctata actatggcaa tatgttttat tgcttattgg tgaagcaata
     2221 cctaactaca acacagtttg acgataaaac agtccaaggg atttttgacg aggcgtttaa
     2281 gtatgaagga acggcttatg tatttggtgg gtcaagaccc gaaacaggat ttgattgtag
     2341 tggcttgact caatggtgtt atgccaaagt tggtctgaaa cttcctcggg tggctcaaga
     2401 tcagtatgat gccatgaccc atattgacct aaaagatgcc aaaccagggg atttggtttt
     2461 ctttcattcg acttatgatg ccggtactta tgtgacccat gtcggtattt atgcagggga
     2521 gaatcgcatg tttcatgcag gagatccagt aggctggacg aatctaacag aaagctattg
     2581 gcagcagcat cttatcggtg ctggacggta caaataagag aaaagaggaa aaagaatgaa
     2641 gataaaaatt gaacggaatc aaaaggaaaa gagcctaaaa aagaaaaaag aacgagtcgt
     2701 atccgtgggt aagcatcgaa aaatggtgct tgccctttgg cttttattat cttgtagtct
     2761 tacttttggc gtttataaaa atttcaccgc cattgatcaa catacaactc atgaaaaagt
     2821 ggttattaaa gagaaagtag tgaatacttc tggtattgaa aactttacga aggatttttc
     2881 gaaagaatat ttttcgtgga aaaataacaa agaagcaatt gaaaagagaa tgaacaacct
     2941 cgaacagtac cttacagaag aaggtttcgc tttaagtcaa gatatggttc gagcagatat
     3001 tcccactaat tccgaagttc agtcagtaaa agtcttagat gttgaaaaga gctcggagga
     3061 atttgtcgtt tcatttttag tggagcaaaa gattacagag ggaaaaaagg cacaatcgat
     3121 ttcttctgca taccgagtga cgatctttga agatgaaaac aggaattata tagtaacgag
     3181 tttgccaact atgattgcaa aacctgatag ggcaaaatac aagacaaaac aagtagaaaa
     3241 tgattcgaaa attgatgcca aaactacaga agaaatcgct gagtttttgg aaacgttctt
     3301 taaattatat ccatcagctt ctgaaaaaga attggagtat tatgttgaaa atgatgcgat
     3361 gcgaccaatc aatattgact taaagttttc gaagttagag gatccagttt acctacaaaa
     3421 cggaaaaaaa attctagtga gtacaaagat aaaatacctt gataatgtat caaaaaccac
     3481 tagctacttt cagtacaaat taatattaca aaaaaatgga aactggaaaa tcattgatgt
     3541 taaataataa tgactcaatc taaatttatg agaagttata agtagtttaa cttatttcag
     3601 gcagaagtcg cccatcctct ataggtgggt gatgaatgcc gttcggtaac tcgaaaaccg
     3661 acaattaaga catagtgcct
//
LOCUS       DACDIP010000130         3594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDIP010000130 DACDIP010000000
VERSION     DACDIP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.133"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1155)
                     /locus_tag="IV257_002747"
     CDS             complement(<1..1155)
                     /locus_tag="IV257_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8815807.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..3407)
                     /locus_tag="IV257_002748"
     CDS             complement(1158..3407)
                     /locus_tag="IV257_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8815808.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
BASE COUNT         1171 a          673 c          512 g         1238 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgatatctt aaaaatgtaa cttatctcct gattaaacaa atgaaattgt
     3361 tggttttctg atcgataaat aatattcatt tcattatcct tatacataag ctctcctccg
     3421 tttctttatt gcattataaa aaacgctcac ataaatagta agcgtttaaa gtaacgaata
     3481 tttatcaaaa tcaaatgttt agtttattca ccattaatga ttcctagacc taatctacgt
     3541 tttcgatata ctcccggtga aacaccattt gctttcttaa aggctttaga aaaa
//
LOCUS       DACDIP010000131         3578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDIP010000131 DACDIP010000000
VERSION     DACDIP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.134"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1087
                     /locus_tag="IV257_002749"
     CDS             5..1087
                     /locus_tag="IV257_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8815809.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IV257_002750"
     CDS             complement(1305..2447)
                     /locus_tag="IV257_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8815810.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IV257_002751"
     CDS             complement(2452..2637)
                     /locus_tag="IV257_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8815811.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IV257_002752"
     CDS             complement(2991..3236)
                     /locus_tag="IV257_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8815812.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..>3578)
                     /locus_tag="IV257_002753"
     CDS             complement(3230..>3578)
                     /locus_tag="IV257_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8815813.1"
                     /translation="KKVLRNEAKSIRKRNANIKDNEEPLADSNEGRYSQTHFDEQDVY
                     FLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDAEIGKILNMSTSGIWYQRK
                     KAVEQLKMGYDLW"
BASE COUNT         1175 a          648 c          547 g         1208 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata ctttttta
//
LOCUS       DACDIP010000132         3523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDIP010000132 DACDIP010000000
VERSION     DACDIP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.135"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            111..335
                     /locus_tag="IV257_002754"
     CDS             111..335
                     /locus_tag="IV257_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP8815814.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(540..860)
                     /locus_tag="IV257_002755"
     CDS             complement(540..860)
                     /locus_tag="IV257_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8815815.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(1008..3242)
                     /locus_tag="IV257_002756"
     CDS             complement(1008..3242)
                     /locus_tag="IV257_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8815816.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
BASE COUNT         1025 a          667 c          660 g         1171 t
ORIGIN      
        1 gattttccgt gacggctctt tttactatat taaaatcata gaaatgtcta atcaaaaaaa
       61 tataaaaatt attttgcgta catatgatta aagaaaggag agtgtgacac atggaaacag
      121 aatggcaaag acgtgaaaga aaaaaacaga aaaaagaaga gatgttacga ttatgtcgat
      181 tattagatgt aaaagactgg gacaaaaacg agcaaattgc acaaaaagta cgatcaatca
      241 tccataaaga ggaaaaagtt aaaaataaag acacgcgtag aactgaaaat gaaaagagaa
      301 aagtacaaaa gaaaagagaa aaatcaaaaa gctagaaaga aaaagaggct gagacagaag
      361 cgtttaactc caagaaataa gctggaattc acaaaaattt gaaaagcaat tttcgtgaat
      421 ttcagcttat ttccgaagga gttgcttctg ctttcgccgt ttatacgtgt ttagagcgtg
      481 agacaaaagt gttctttact tttgtcccac gctctttaat tattatgttc aaaatttgtt
      541 tacacggcta atgtagcgcc taattcatgt tttggaatcg agatttcaac aaggcctgcc
      601 aattcgatta ctttgatttc atcctcgtgg taaacggcgt ctttgatttt atctaagaaa
      661 taatttttag catcttcgat ttctttcggc tggattttac gtttgtcatt gatcagcaga
      721 atttcattat gttcaggggc ttcagtgtag acaatagtgg tatttccggc tgtgtagacc
      781 ttgactagat gggcatcagt gttttcaagc tggtttaata caagacggga atggctgttt
      841 gttacattta ccaatttcat ggcgctcacc tcttttatgt atttttaatt gcttgtttct
      901 cttattataa tttctttttt ataaaatgaa cagtattttg acttttctga aaaaagaatg
      961 agacggcatt tctcaaagaa ataccgtctc acgctataaa actggattta ttcagccgtt
     1021 tcttcggctg attctttagc caaacgttct ggtttagaag taacgatgat ttttccatct
     1081 ttatccagtt ttgctttcaa ctcatggata gaagggtgat caagataaaa ttcagcgata
     1141 ccatcttcga tttgttcttg gatcgtgcgt cgaagaggac gagcacccat agatggatca
     1201 tagcctaagt caactaattt ttctttgaca ttcgtaggaa cttcgatgtg aagttgctgt
     1261 gctgccagca tttggttgac atcgtctagc attagtgtga cgattgtcat caagttttct
     1321 tttgataatg cactgaattc gatgatcccg tcaaaacggt tcagaaattc tggtgtaaag
     1381 tagttgttta attggttcaa tacagaacga gtaacacctt cgcgtgccgc accaaaacca
     1441 acgtttgctt ctactttacc agttccggca ttacttgtca taatgatgat cgtatcttta
     1501 aagctgaccg ttcttccttg tgcatctgtc aatcgaccat catctaagat ttgcaagaac
     1561 atatgcaaaa catctggatg agctttttct acttcatcta acaagacaag actgtaagga
     1621 tttctccgta ctttttctgt taattgacca gcttcttcgt atccgacata acctggagga
     1681 gaaccaatca atttagagac actatgtttt tccatatatt cagacatatc gaaacgaatc
     1741 attgaatctt gtgaaccgaa tagttcgtag gcaagttgtt tcgcaagctc tgttttacct
     1801 acaccagttg gtccaacaaa taagaaggag ccgattggac gattctgttt gtttaatcca
     1861 acacggtttc ggcgaatcgc tttcgctact cgatctacag cgttatcttg tccgatgaca
     1921 tgagctttca agtcatctgc taagtttttc agttgtgtct gttctttttc ttttaattca
     1981 cccactggaa tacctgtccg ttgttcaacg attttttcca tatctttttc ggtaatcaca
     2041 ggtgtttcct cttcagtgag ctgacgttct ttcatttttt gaagtttatt gatctgatcg
     2101 cgataataag ctgctttttc gaaatcttct tctcgagaag ctaaaacttt ttgttcttct
     2161 gcttcttgca gttttttctc gattgtttta ggatcaacga tttggatcgt caagtttttc
     2221 ttagaacctg tttcatctag caaatcgatg gctttatccg gcaagaagcg atcttggacg
     2281 taacgattgg ataaagttgc ggctgcttcg atggcctcat cagtatattt tacatgatga
     2341 tagtcttcat aacgtttttg caaacctttc aaaatactga tcgtttcttc gacagttggt
     2401 tcatctaccc gtacaggttg catacgacgt tctaaagcag cgtctttttc gatgatgcgg
     2461 tactcattta aggtagtagc accaaccatt tgcaattctc ctcgagctaa agctggtttt
     2521 aaaatattac cagcatccat atttccatca ccagcagaac cagcaccgac gatttcgtga
     2581 acttcatcta tgaataggat cacattttct gcttgacgga tctcttcaat caatttttgc
     2641 atacgttctt caaattgtcc tcgtatacca gtaccttgga ctaaagatac gacatctaaa
     2701 cgaatgactt ctttgtccat taatttttgc ggtacatcac catctacgat tttttgtgct
     2761 aaaccttcaa caactgctgt tttaccaaca cctggttctc caatcaagac aggattgttc
     2821 ttggttcggc gatttaaaat ttcaatgact cgtttgattt cgtcatcgcg tccgatcaca
     2881 ggatcgatat caccttgtcg tgcttgttct gtgatattga ttccgtattc tcccaataaa
     2941 ccttctgtat tgccttgagg tggctgacca ccattgaagt tgttgtttcc gccaaattgt
     3001 gttggtggtg tttgctcata atttccttgc tgttgcattt ggcgagacat ggaacggaaa
     3061 agatcgtcca aactgccaaa tccaaatgga tcattttggg ccatattagt cagacctcca
     3121 ttttgttgat ttttgatttt ttgataacag ctctgacagt aatcgagttg tgtccgctga
     3181 ccattgacag tggcatacaa gtgaatcgtt gcctcattct tgccgcagtt ttgacaaagc
     3241 atgaatcatt cacgtccttt cacaaaaatg ggtacatcca tattttacat ctcgtttttc
     3301 atgaggtaaa agaataaccc ctagtttttt aagagattta tgaggtagaa aaggtgaaat
     3361 ctctattgac cattcctgac ctttgactct tattatacta aaaataaaaa gggaagcaag
     3421 taattcgttt catatgaaaa caaagagggt caaaggaaag aatgataaac gtcaggagaa
     3481 gagaaagcga caagaaaaag gaaataaaca cgtttgttat aaa
//
LOCUS       DACDIP010000133         3458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDIP010000133 DACDIP010000000
VERSION     DACDIP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.136"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            555..1994
                     /locus_tag="IV257_002757"
                     /pseudo
     CDS             555..1994
                     /locus_tag="IV257_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2339..2506
                     /locus_tag="IV257_002758"
     CDS             2339..2506
                     /locus_tag="IV257_002758"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8815817.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            2765..2980
                     /locus_tag="IV257_002759"
     CDS             2765..2980
                     /locus_tag="IV257_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815818.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3168..3455)
                     /locus_tag="IV257_002760"
                     /pseudo
     CDS             complement(3168..3455)
                     /locus_tag="IV257_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1045 a          573 c          716 g         1124 t
ORIGIN      
        1 tatttaggat cgtatgattc tattaagagt gcagctcaag ttagaaaaga tgctgaaaat
       61 aaattgtttc aacctttgat tgacaaatat cgaggtaatc attaattatt gtggataata
      121 attaacagtg atatataaac atctataata tattattcat actattgaca tttagtataa
      181 tcctatggaa ttgaatatca tagcaatgta tttgaaaagc aaattggaaa acaaataggt
      241 gcaacaccat aaatatgaaa aaattgggaa aaagtagaat ggaaattttc ttacgtctca
      301 cgagatagct cacactatta atctgaagaa caatgaagtc gacttttaga aattgctaga
      361 agaaagtaaa gcgaattatc actagttact atagagtaag ttcaaataaa gcatctcaaa
      421 cttacatggt tacatgagaa taacaaatcg aaattactca agcttcctat ggctagagtc
      481 gcgagaagtt gttgggcagt agacataggt tatattgttc ttagctacac gatcattaga
      541 aaggaaatcc tattatgcat agttatcaag aagatgcaaa agtccaaaaa aatcgctggt
      601 ggattttgat tgctatatct atgtttacct tcatgtccac attggatggc agtattgtca
      661 atattgcact acctaccatc tcagatgata tgggcatacc aatgaatcaa gcggaatggg
      721 tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt gctgtttggt aaaattggcg
      781 atagctgtgg gaaaatcaaa gtatttcgga ttggtacgct gatctttgtc ttgggctcgc
      841 tgttgtgtgg gttcaatcat tcgctaggta tttaattgtt cgcacgggtg gtacaagcca
      901 ttggtgctag tatgactatg gctacaaatg ccggaatcat cacggaagtc tttcccatac
      961 atgaacgtgg tcgggcattg ggagcaatcg gtgcgcttgt ttcattaggc tcgattgctg
     1021 gaccaggtct tggcggcttg attttgtccc aattttcttg ggcgtacatt ttttggatca
     1081 atgtaccggt gggaatcgta acgatgttga ttgctgaaaa attcttacca aaagatatca
     1141 ccatgagcgg ccatacaatc gatggagggg gctttgctgc cattgcggct acgattatca
     1201 gcctttttgg aacaatcttt ttggggcaag aaagtggctt tttccatcca gtcccccttg
     1261 tactgttcgt aatttcagtg attgcctttg tcagcttttt aaaagttgaa aagaaagcag
     1321 cctctccatt gatttctttc ggtattttta agaacaagat ttttaccatg agcttgatta
     1381 cagccttgtt gattttttct tccaacttct ttgttaatgt agtgattcct ttttatctgc
     1441 aaaatgctcg cgggttgagt cctagctatg ccggactgtt gatgatggtc tttcccttga
     1501 tgatggtcgt cggggcgcct ttaagcggat acctgaccga ccgggttggc ccggagattt
     1561 ttgtattagt aggcttgggt ttattgtcgg taacccaagt catgtatatg tttttgtcag
     1621 tggactctcc tttgtggctg tacattgtcg ctactgccat catgggtgcc ggcaatgcct
     1681 tgttccagtc cccaaataat acgatggtta tgagttctgt cactatcgaa aatttgggac
     1741 tggcaggctc catgaattcc tttgcccgaa atttggggat ggtcatcggg atttcgcttt
     1801 ctacgacggt cttatatcaa gcgatgtctg ctaagatggg ccaacgagta actaactata
     1861 tcgcagatcg gccggatgtc tttttatatg gaatgaagat taccttttta ggcagctttg
     1921 ttttgtgttt tgttgccttt ttcctgacta tctggcgtat caaaaaacaa ggaggtattc
     1981 agaaaaatgt ctgaaaaacc attgttgaag aatttttgga ggcctgcgta tgggtgagtg
     2041 ggatttccat ggatgatgga agatgaagtg aacttctgaa aaccgaaaaa ctcaagtatt
     2101 atcttcaaaa tactagtact caattagatg ctcgatttga ttacaagaag actgcgaaag
     2161 ccactggcaa ccgcaagcta ggtatccacc gatggactac gttttagagg caactggtta
     2221 tgaatacgga gctaacgctc tcatctgcat aagaatgcaa cacccagatt atgtaaagat
     2281 tttcgatact tctgtcaaaa aaatcatcaa gatagataaa tgatttccgt ctagtcaaat
     2341 tggttcagaa tgtggacaca cagatggtaa gaagtcattc acaattcgag aatggacttg
     2401 tcctatttgc cgtacacatc atgatcgaaa cgtcaacgtt agtatcaata ttttgatcaa
     2461 gggctatgta tacaagcatt gccttagaaa acatcaagaa ccttaggaac aacggggata
     2521 gattggttaa taagagatat cgctgttagt aaagacatag gcttgcaagt atactctttt
     2581 atcaagaatt ccgtggcttt agtcatgata ggtttaatac tatgaaaatt ttctttagca
     2641 tttttgattt aggttccttt tctaattata ataaaatgac cactacagta acctactgaa
     2701 atatataaac agccactgta gtaccatttt caaagtagtc tatgagagga gaatcttcat
     2761 tcttatgaac gctggaattg cggatatgaa tttaataaaa aaaaccctta atgattttac
     2821 ttccaattca atatcaaaag gtactggtat caatttaagc acaataaaaa agctgaaaag
     2881 tggtgaaaga tcagtggaaa agctgaatct tttagatgct ataaaaataa cggaatttgc
     2941 tatgaaaaat ggaaaagctg agattgaaat atggagataa ttatcgtcta gtgaactact
     3001 tgccacttag ctgaatttaa cgatccttag tgcttgaagt gggagattct tgggaataga
     3061 gcatacttgc aagcttatgt ctttattaac agcgatatct cttattaacc aagctatccc
     3121 cgtagttcta aggtttttac ttttatctaa gctaatgatc gtattcaaag gaaatttttg
     3181 acccttagaa agtctcattt tatcagacat gagcttaggc attgctgaat tttgttaatt
     3241 cggttttagg ttgtgctttg agcataatat gaaagtagtc tttatcacga tttcactcaa
     3301 ctaaggtaat gtgatgtgat tctgatattc tttcaaagat agttttttgc ataagctaac
     3361 attacatcat caatgagatg tttacaatgt ttcactacta aaataaggtg gtaacaaaga
     3421 agaaataatg aatgattatt tgtatctaat tccatggt
//
LOCUS       DACDIP010000134         3388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDIP010000134 DACDIP010000000
VERSION     DACDIP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.137"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..884
                     /locus_tag="IV257_002761"
     CDS             <1..884
                     /locus_tag="IV257_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8815819.1"
                     /translation="SGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLD
                     IEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIA
                     NKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTY
                     SIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSL
                     FLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFA
                     SSLMLIKKPKEILSKIS"
     gene            894..1550
                     /locus_tag="IV257_002762"
     CDS             894..1550
                     /locus_tag="IV257_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815820.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            1550..2926
                     /locus_tag="IV257_002763"
     CDS             1550..2926
                     /locus_tag="IV257_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8815821.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3056..>3388
                     /locus_tag="IV257_002764"
     CDS             3056..>3388
                     /locus_tag="IV257_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8815822.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1344 a          378 c          595 g         1071 t
ORIGIN      
        1 gtagtggagt atttacaatt aaagaaggta aaaatataga agaaaatgat aagaattcaa
       61 ttattgttca tgaagaattt gctaaacaaa acaatctaaa attaggtgat gaagttgatc
      121 ttgaattact agatattgaa aaaagtggaa aaataaaaag tcataaattt aaaattatag
      181 ggatcttttc tggtaaaaaa caggaaacat atacaggatt atcttctgat tttagtgaaa
      241 atatggtgtt tgtagattat tcaacaagcc aagagatatt aaataattca gaaaataatg
      301 aaattgcaaa taaaatttta atgtattctg gtagtgcaga atctacagac ttagccttaa
      361 acaaattgaa agagcttaaa attgatgagt caaagtattt tgttgaaaaa gattctaatg
      421 cgtttgaaga gtctttagag tcagtgagtg gaataaaata tataataaaa gtaatgactt
      481 attctattat gttaggtggg atggttgttc tttcattaat cttgattcta tggttaaggg
      541 aaagaattta tgaaataggt atattcttat ctattggaac atctaagata caaattataa
      601 tgcaatttat attcgagtta atattcatat cgataccaag tataatatct tccttatttt
      661 taggaaatgt attactaaaa gtaattgtag atggattcat taactcagag gattcaatga
      721 tttccggtgg aagtttaata aataatagta gttttatgtc aaatataaca acacttggac
      781 aaagttattt aatattaata agtattattg ttttatcagt tgtatttgct tcttcattaa
      841 tgttaatcaa gaagcctaaa gaaatcttat caaaaataag ttaggagaaa ataatggata
      901 tattagaaat aaagaatgta gcatatagtt atgcaaattc taaagaaaaa gttttgtcag
      961 gagtaaatca aaaatttgaa cttgggaagt tttatgcgat agtaggaaag tcaggaacag
     1021 gaaaatctac acttctttcc ttacttgctg gacttgataa acctcaaaca ggaaaaatat
     1081 tgtttaagaa tgaagatata caaaataaag gatatagtaa tcatagaaaa aataatatat
     1141 ctttagtatt tcaaaactat aatttaatag attatttatc accaattgaa aatattagat
     1201 tagtaaataa atcagcagat gaaagtatct tgtttgaact aggtttagat aaaaaacaaa
     1261 taaaaagaaa tgttatgaaa ttatctggtg gacagcaaca aagagtagct attgctagag
     1321 cattggtatc agatgctcca ataatactag ctgatgagcc tactggtaat ctagacagtg
     1381 ttactgctgg agaaataatt aatatattaa agacattagc taaagataga aataaatgtg
     1441 taatagttgt aacacatagt aaggaagtag cagattctgc ggatatcatt ttagaactaa
     1501 gtggtaaaaa gttgaaaaaa gtaaataaaa tgaatttgga ggttgaataa tgataaaaaa
     1561 tgcatttgct tatgtaacta gaaaaagttt aaaatcatta attatcatat tagttatttt
     1621 atctatgtca actttaagtc tcattagttt atctattaaa gatgctacgg acagagcttc
     1681 aaaagaaaca tttgctaata taacaaatag tttttctatg gagataaata gacaagtaaa
     1741 tccaggaaca cctagaggtg ggggaaatgt aaaaggtgaa gatattaaaa agatatctca
     1801 aacagatagt atagactcct atgtaaaaag aataaacagt gttgcagatt tagttgatta
     1861 tgatattatt gaaactcaag agactcttgc gaatcaatca cctgaaagag caaagaattt
     1921 taaaagaaca gttatgttaa ctggggttaa cgactcaaca aaagaaacaa aatttgtatc
     1981 agaagcatat aaattagtag aaggaaagca tttagaaaat gaagataaaa ataaaatctt
     2041 aatgcataaa gatttagctg aaaaaaataa ccttaaagtt ggagataaga taaaaataaa
     2101 atctaattta tttgatgctg ataatgagaa aggtgcagat gaaacagtag aagtagaaat
     2161 taaaggtcta ttcgatgggc ataatagcgg tgtagtaagt gcagcacaag aactatacga
     2221 aaatacatta atcactgata ttcatacagc tgctaaagtt tatggtaata ccgaagatac
     2281 agctgtatac caagatgcaa cattctttgt aaaaggtgat aagaatcttg atagtgtaat
     2341 aaaagatctt gggaaattag atataaattg gagagaatat aatttgatta aaagttcatc
     2401 aaattaccct gcattacaac aatctatatc aggtatttac tcaatttcaa ataaattatt
     2461 ttttggctca ttaatatttg caggagttgt agtttcatta ttattattct tatggatgaa
     2521 tgctagaaag aaagaaatag cagtattact atcattaggt atatcaaaat tagaaatttt
     2581 tggtcaattc ctaattgaga tggtatttat atcaatcccc gcattcgttg gttcctattt
     2641 cttagctcaa tatacagctg acaagcttgg gaataatata ttgaataagg ttactggtga
     2701 tatagctaaa caaatagcta aacaatctgc atctagccaa ttaggtggtg gggcagaagc
     2761 tgaaggattt aacaagacat tatcaagttt agatatcaat gtattaccta aattcatgat
     2821 ttatgtagtt atatttatga gtttagtact tcttgtatca ttgattattt cttcattcaa
     2881 tatattaaga aggaatccaa aagaattatt aattgacaat aattaaaatt ttcggtgctt
     2941 tttagcacca aaaatgagag tgtaatataa accgtgtaag taaccatcaa tcctaggcgg
     3001 gtggttgctt cacggttttt atttttctaa aataagatca agaggtgatt acacgatggc
     3061 tagaaagaaa agaaatcctg atgctgagaa gttagctgaa tccattttga atacttatca
     3121 acccgaatct gtagaggata tgcaagatgt tttaaaagat gtgtttggtc ccttatttga
     3181 aaaaatgctt caaggagaat tgaataacca tttagggtat gacgcccatt ctaaagagcc
     3241 aaagaaacat gacaatcgac gaaacggtta tggcaataag acgcttaaaa cgagctttgg
     3301 taaagtaaac attgatgttc ccagagaccg ggaggcttct tttgaacctg agttgattcc
     3361 taatagaaaa cgagatgtct ccgacatc
//
LOCUS       DACDIP010000135         3351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDIP010000135 DACDIP010000000
VERSION     DACDIP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.138"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(551..2164)
                     /locus_tag="IV257_002765"
     CDS             complement(551..2164)
                     /locus_tag="IV257_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8815823.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="IV257_002766"
     CDS             complement(2305..3213)
                     /locus_tag="IV257_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8815824.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1093 a          568 c          581 g         1109 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
     3241 tttctattta taattaatga ctattataac aaaaatgaaa agccaaaacg acaagtaaaa
     3301 tattttttta tataaatatt tgtgttacta attccgttaa tatacataag t
//
LOCUS       DACDIP010000136         3221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDIP010000136 DACDIP010000000
VERSION     DACDIP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.139"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            460..1128
                     /locus_tag="IV257_002767"
     CDS             460..1128
                     /locus_tag="IV257_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8815825.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            1119..2252
                     /locus_tag="IV257_002768"
     CDS             1119..2252
                     /locus_tag="IV257_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8815826.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2547..2906)
                     /locus_tag="IV257_002769"
                     /pseudo
     CDS             complement(2547..2906)
                     /locus_tag="IV257_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1016 a          532 c          683 g          990 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaacctgctg cattattagg ggaacgaatt ctaaaatagt aagtgagtta aaacgaaggg
      121 gtttttcttt agagagattt tcttacatgt ggtacttaat gacttgatgg caattttcag
      181 tattctaacg aggcttattg gtcataaacc aacgaacttt gaaaagtata tacaagaaaa
      241 ccaagcaaat agaaagtagt gagactggag gaaaggttcc agtctttttc tattctaggt
      301 attttcaaac agtaaaatca ttcttaatta tgtgtttttt aacagaatgt atgaatttct
      361 tcaaatcttc acaaaaagaa cgtggacttt gaagaagagc gtgtactatg ctaaaaagca
      421 taagtgtaat tactaaatcc gtatgaggag tgaatataca tgcgtgtact ggtggttgag
      481 gacaatcgtg agctggtttt atccgtaaaa aagggcctag agcaggagaa tatttctgta
      541 gatgtagctt atgatggcga gtcaggagaa gagaaggcct tcatcaattc atacgatggg
      601 atactactag atttaaatct gcctaaaaaa gatggattat ctgttttaca aagtctccgt
      661 gctgaagaag tcgaaagtcc agttatcatt attactgcac gtgatgagat tgaagaacga
      721 gcaaaaggct tggattttgg agcagatgat tatttagtca aaccctttga gttactagaa
      781 ttaaaagcac gaatacgcgc ggttattcgt cgttatcatg gacgtgcgct gccgcagatt
      841 tcaattggtg ctttgaagat tgatccatta ggtcgaaggg tcagctatca aaagacagag
      901 gtggtcctga aacccaagga atttgatatc ttactgtgca tcgctcagcg tcatccagcg
      961 gttgtttcgt ctgaggaaat ttctgaacat gtctacgatg aagattttga tcctttttcg
     1021 tcggttctgc gagtgcatct ggctcgtcta aaaaagaaac tggctgaagc agctgaaaaa
     1081 gaggtcttaa aaaccattcg agcaaagggg taccagctat gctattaaat tttaaaatga
     1141 cgttaacgat gctggctttt tcactgttgg tgacaggcgt gattttagtg agtttcttct
     1201 ttctgagaaa taatttggag ggaatcagta tcgcatctac cttaggtgaa gatgtttatt
     1261 ttatggaaga gggtaagacg gttcaagcag agacgcagat ggaagcttcg aaatcttcca
     1321 acaatcaatt tatgactatt tcgatgatag ataaagctta ccaggacgaa ctatttaaat
     1381 atttaccagc gatggtgctg gttatttgtt ctgccgtcat gttattaacg tttattcttt
     1441 ggttggtttt acgcaaaatt tacagtaatc aaatcttagc gattacgcgt gatttaacgt
     1501 ttattgatca agttcctaaa aatactgtga aggacgaggc attaaatgcg gcttttaata
     1561 agctaaagga taagttcgat ggtcatttgg aggattatcg acgattaaat agttatctga
     1621 cccacgagca gaaaaatgcc atcgctattt tgcgtacgaa tctggaaata gaacggcaac
     1681 atgccgatca aggaaattta atgcttttag atcggatgac cgacagtatt gacgatattt
     1741 tgacattatc caataactca gaggatctta gcacggaagt cgtagacgtt tcactgatct
     1801 gtgcggaagt ttgtgatgcc tacaaaatga attatccgaa tcttttgttt gcgtttactg
     1861 aagatcaacc aaccctgatt catggacgag atcgttggat ttatcgggcg gtttcgaatt
     1921 tagttgacaa tgcaatcaaa tatggtgaag gaaaactggt aattgtcacc atttcaaacc
     1981 aaaagggtag cgtgattatt gaagtaaaag atcagggaat tggcattcct caggagaaac
     2041 aggcatcaat tttttctcat cagtaccgag tgcatgggct caagcaggat ggcttaccta
     2101 aaacaatgga ctttagggac acggatgaaa cctttattca atccgttgcc tcgaaaagaa
     2161 accatattcc aagaaaatca ttaaattatc gaacaccatt ggaagtattt ttgagctgca
     2221 tagataaaga catattgtct agcttaattt gacaaatcag atgtctataa aattcgttag
     2281 aaaatacaat ttatagatat ccaattgatt tgtaaacaaa aaggagttgt caactaagcc
     2341 actccttttt gttgtaattt ttcccaataa gaaagtctac tactttagga agataatagt
     2401 aagatagaaa tgttgccccc aaaattatta tccagctgga tataattaat caaataaaaa
     2461 ggatcaaaaa tgtagtgacc cccaaaagtt agactttttt ggagtggaga aatccactct
     2521 ttttttcatg ctctaaaatg atatttttac acaaccgtaa cggttgtttt acgaaattgg
     2581 acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta atagatatag
     2641 ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg aaaaatctct
     2701 tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt ccctttccga
     2761 gacatgcttt gaaagatttt gtactccttc aatttgtttc tataagcttt catttgatac
     2821 gaccaacctt gatctgagtg aaacgtacga cgatagggac aatcgttagt ggtctggata
     2881 gcttcctcca aaccttccat taccgcacgt gtttctgaat ggtggtagcg accgatttga
     2941 acggctaggg cggaaatacg gtgcaaatgc acggtggttt catgcaatgc ttacagggcg
     3001 tattttgtaa gggtatgtaa cggagataat cccgacggta atggatgtga gaggccaagc
     3061 cgacgctgac ggtagggcaa acaaacatag ttgcactcta gcgatcgtta tttattttta
     3121 acggtcaaac tagggtacaa ctatgcttaa aacacttcgc ctcaacttct ggtagcaaaa
     3181 gtgattttga aagtcaaaat attttttatg agttatttat a
//
LOCUS       DACDIP010000137         3193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDIP010000137 DACDIP010000000
VERSION     DACDIP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.140"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..1261
                     /locus_tag="IV257_002770"
     CDS             2..1261
                     /locus_tag="IV257_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8815827.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            1271..2143
                     /locus_tag="IV257_002771"
     CDS             1271..2143
                     /locus_tag="IV257_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8815828.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            2155..2985
                     /locus_tag="IV257_002772"
     CDS             2155..2985
                     /locus_tag="IV257_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8815829.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
BASE COUNT         1030 a          483 c          614 g         1066 t
ORIGIN      
        1 aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca ctaggagcaa cgatctcttt
       61 gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag gtagaaatag agttttttaa
      121 tcaaaaaaaa gaaatgactc aaacgattca ggagattgct aaagattttg aagcaaaaaa
      181 ccctgatatt catgtaaaag ttgtggatgt accgaatgct ggtgaagtaa ttaaaacacg
      241 tatgcttgca ggcgatgtac cagatgtcat taatctttat cctcaaagta tagaattgaa
      301 agaatgggcg aaagctggtt atttagagga cttaaccgaa gaaccgtatt tagagaatat
      361 caaaaatgga tatgcacaac gttttgctat tgaggatcgt gtatatagca taccattaac
      421 cgctaatgtc tatggttttt actataacaa aactgctttt gaggaaatgg ggattcaagc
      481 accagaaact tgggcagaat ttgaaaaaat agtagctgag attaaagatc aaaacaaagt
      541 accgtttgcg attgcaggag cagaaggctg gactttaaat ggttatcacc aactagctat
      601 agcgactgct gctggtggtg aagaggaagc gaacaatgtt tggcgtttct cagaagttaa
      661 tggaattaat gctgaaagca aagaaatgca aaaagatttt aatcgattag atttgttaag
      721 agaaccaaaa gctttgcaaa acaactggca aggtgccggt tataacgata cagtagttac
      781 ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca tgggcaatgc cgatgattca
      841 ttcacaaaat ccagattttg aagtaggaac ttttcctttt ccagcagatg aagcaggtca
      901 aagtctaacg attggcgcag gagacctagc cttatcagtt tctgcaactt ctcaacataa
      961 agaagaagcc aaacgttttg tagaatatat gacaacacct gaagcgatgc agaaatatta
     1021 tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc gagaatactc aggattcacc
     1081 attaagtggt ttaactgaac ttgcttttac tgatcgtcat ttagtttggt tagccaaaga
     1141 ttggaatagt gaaaatgatt tttatacctt aacaacaaat tatttacaca atggcaatca
     1201 aaaagccatg attaacgcat taaatgcttt ctttaatcca atgaaagctg acggaaggta
     1261 ggaaaataaa atgaaaataa aagaaatttt aaatcgttac tgggcaatca tttttgtagc
     1321 aattccaatt attttgcaga tgattttttt ctactttcca atggttcaag gtgcttttta
     1381 cagtttaact aattggactg gtctgactta taattttgat tttgtgggtt tgaacaatta
     1441 taagattctt ttttctgatc cgaagtttat caaatcgatt ggcttcactt taattgtgac
     1501 gctttgcatg attgctggac aaattggttt tggtattttg attgcgcggg cgttgaacgc
     1561 aaatattaaa gggaaaacat tctttagagc atggttcttt tttccagcgg tcttatcggg
     1621 cgtggttgtt tctttagtat ttaaacaaat ttttaactat ggtttaccaa aaattggtag
     1681 tatgttaggc attgaatttt tacaaaccag tcttttagga acaacatggg gagcgatttt
     1741 tgcagtaatt tttgttttac tttggcaagg aattgccatg ccagtcatca ttttcttagc
     1801 gggttttcaa agtattccac aagaaattat tgaagctgct agaatggatg gggcgaatga
     1861 taagcaaatt ttttggaata ttgaaatcac ctacttatta ccaagtattt caatggtatt
     1921 tattatggca ttaaaaggtg gcttaacagc atttgatcaa atttttgctt taactggtgg
     1981 tggaccaagc gatgccacaa catctttggg gttacttgtt tataattatg cttttaaaaa
     2041 caatcagttt ggttatgcga atgctattgc tttagtctta tttatcttaa ttgggattgt
     2101 ttcactggca caattaaaat tatcgaaaaa atttgaaatc taggaggttt cccaatgaaa
     2161 acatcaaaag aaaaaaatat ttctaaatac ttgcttttat tattaggggg cttcatgatt
     2221 ttagttccct tagtttcaac gctattcggt tcatttaaaa caacacccga tattatgaat
     2281 aatttctttc gttttcctaa tccagcgact ttggaaaatt tccgaagatt gttagcggat
     2341 ggaattggtc attacttttg gaattcggca atcatcacaa tcattgcagt agttgctgtg
     2401 acagcaatta ttcctatggc tgcttattca ctagcaagaa aaatgactcg tgggaaagca
     2461 tacggattga tgtatttatt tttaatttta ggaatttttg ttcctttcca agtaattatg
     2521 attccgatta ccgttatgat gagtaaaatt ggtttagcaa atatgtgggg attaatctta
     2581 ctctatctca cttatgccgt accacagaca ctatttttat atgttggttt tattaaacta
     2641 aatgtgccta tcagtctgga tgaggctgct gaaattgatg gggctagtca ctttacaact
     2701 tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg cgacgactat tattcttaat
     2761 gctctatggt tttggaatga tttcatgcta ccattattaa ttttaaatcg tgattctaat
     2821 atgtggacac taccattgtt tcaatataat tatacgggac aatattttaa cgactatggt
     2881 ccaagttttg cttcctatac agtaggaatt gtcacgatta cactagttta tcttgctttc
     2941 caaagaagta ttatagctgg aatgagcaat ggagcagtga aataaccaat aaattattct
     3001 cattataaag gagttcataa acttatgatt atatttgaca atcacacaca aacatttcat
     3061 ttacaaaata aaaaaattag ctatctatta ggaatcgaag aaaataatta tttaacgcat
     3121 ctttattttg gaaaaaagat tacgcactat tctggtgggt atcgctaccc ccgaacagat
     3181 cgttccttct cac
//
LOCUS       DACDIP010000138         3149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDIP010000138 DACDIP010000000
VERSION     DACDIP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.141"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1975
                     /locus_tag="IV257_002773"
     CDS             <1..1975
                     /locus_tag="IV257_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8815830.1"
                     /translation="TLPLEFPGYGHGDFREPAHNIKLGNGSRVNDFRYDSYEIQKGKP
                     SLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVIIRSTQLVNNSDET
                     VEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLDSKRGSSSHQQNP
                     FVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGINSFNFGWQLKTGD
                     SFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLINNWEATYFDFNEE
                     KIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLACGLENLAKQVHDN
                     GMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDFSRQDVRDNIYQQ
                     MTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLYEFLEKLTTNYPT
                     ILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVYPISSMGAHVSAV
                     PNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAFYKKHRQLLQYGT
                     FYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLAGLAEEATYQMED
                     VTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            2036..>3149
                     /locus_tag="IV257_002774"
     CDS             2036..>3149
                     /locus_tag="IV257_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8815831.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQ"
BASE COUNT         1084 a          479 c          663 g          923 t
ORIGIN      
        1 cacattacca ttagaatttc caggatatgg acatggtgat tttagagaac cggcgcataa
       61 tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct acgaaattca
      121 aaaaggaaag cccagcttag cgggattgcc agccttatat acggaagatg atgatgaagc
      181 tgaaacgctg attattcaat tgaaagacgc gattagtgga ttgactttac agttgttcta
      241 cacaatttat caaaatcaca gtgtaattat tcgttctacg caattagtaa ataatagtga
      301 tgaaacagtg gagattaacc gcttagctag tcaatcaatt gattttccaa atcgtccact
      361 cgagttaatt catttaaatg gtacctgggc aagagaacgt caaatgacta gagaaaaaat
      421 tcatacaggg ataaaagtat tagatagtaa acgcggatca agcagccatc aacaaaatcc
      481 gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat atggatttaa
      541 tttagtttat agcggtaatc acgaaacggt gattcaaaaa gatccatttg accaaacacg
      601 ggtcattatg ggaattaatt catttaattt tggttggcaa ctaaaaactg gcgactcatt
      661 tcaaacgcca gaagtcgtga tggtttatag taatgaaggt ctgaatacgt tatcacagac
      721 gtatcatgaa ttgtataatc accgtttaat tcgtggaaac tatcgtttaa aagaacgtcc
      781 cacgttaatc aacaactggg aagcaacgta ttttgatttt aacgaagaaa aaatcatgaa
      841 tattgtcgat gatgcgaaga acttgggcat tgaaatgttt gttttagatg atggttggtt
      901 tggcggtcgt gaaaatgact tcacttcatt aggtgattgg gttgaaacag aaggcaaact
      961 tgcttgtggt ttggaaaatt tagccaagca agtgcatgat aacgggatga aatttgggat
     1021 ttggtttgaa ccggaaatga tttctgaaga tagtgaacta tttcgagcac atccagattg
     1081 ggcattgggt attccaggtc gtgaccgctc aattagtcga aatcaatatg ttcttgattt
     1141 ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg acggcgattt ttgatcgtgt
     1201 accgattgat tatgtgaagt gggatatgaa tcgaaatatg acagaagtct attctgtctt
     1261 gttggactca acgatgcaag gtgaagtggc tcatcgttat atgttagggt tatatgaatt
     1321 tttagaaaag ttaacaacga attatccaac tattttattt gaaagttgtt cgggtggagg
     1381 cggtcgtttt gatgcaggtt tcttgtatta catgccacaa acttggacga gcgacaatac
     1441 cgatgccgtg gctcgtctga aaattcaata tggaaccagc ttagtgtatc caatttcaag
     1501 tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa cgagtttaac
     1561 tattcgtggc aatgctgcga tggctggtgt ttttggttat gagctagatc taacgcaatt
     1621 aacttctgaa gaaaaagcag aaattaaaga acaagttgcc ttttataaaa agcatcgcca
     1681 acttttgcaa tatggaactt tttatcgctt ggaaagtccg tttgaaggca atgatactgc
     1741 atggatgttt gtctcaaaag accaaacaga agccatggtc ttttatttcc gagtattagc
     1801 agaagctgcg catccattag tcacattgaa attagcagga ttagcagaag aagcaactta
     1861 tcaaatggaa gatgtaacat ttgctggcga tgaattgatg aatctcggtt tttatattga
     1921 ctcagaacta catggtgatt atgcgacaca acgcttttat ttgaaacaga tatagtagct
     1981 tgaatttaca atataagtgc aacaccaaaa gagactcatt ccaaacgaag aaaatatgtt
     2041 ttatttacac gcatttacta aaaagcagcc tgatttgaat tgggaaaatg aagaagtacg
     2101 tgaagaaatc tatgcaatga ttaactattg gttagataaa ggactaggcg gatttagaat
     2161 cgatgcgata ttaaacatca aaaaaaaact agagtatggc atctttgaat ctgatggaga
     2221 agatgggtta gccttcattg ggccctggat tcttaatcag tcgggcattg aagtgtggtt
     2281 aaaagaaatg agagagcgga catttaaacc acataatagt atgacggtag cagaagccga
     2341 tgttcctaat gaacgtttag atgaatatat tggtgaagat ggctattatt cgatggtttt
     2401 tgattttagt tatacggata ttgatgtacc tgaaactggt gaatggttta aagatagcca
     2461 atggacatgg acggatatgc gtaccaatat ttttacgaat caattagtta cgcaagataa
     2521 aggttggggc gccttatatt tagaaaacca cgatcaacca cgttcaatta ataaatatat
     2581 tccagaagaa tggatcaatg attatagtaa aaaaatgttg gcgacgcttt ttatgttact
     2641 aagaggtacc ccttttattt atcaaggaca agaactagga atgaccaaca tagaaatggc
     2701 atcattagaa gattttgatg atgtagctac gcattctcag tataaacggg cattagaata
     2761 tggattggca ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg ataattctcg
     2821 cacacctatg caatggaata cgcaaaaaaa tgccagattc tcaacttctg ataatgtatg
     2881 gttaaaagta aatcctaatt atccagaact caatgcagaa gctcagttaa ccaatcaaca
     2941 gtcggtattc aattattatc atcaattaat taaattaaga aaaaatagtg tatacaaaga
     3001 cgtgctaatc tacggacaat ttctaccagt gaaagataaa aatgaacata ttttgcttta
     3061 tgaacgccaa ttagaagata aaatcgtatt agttatctta aacgttacag caacagaaca
     3121 agaatattat gtagatgaac agtaccaac
//
LOCUS       DACDIP010000139         2976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDIP010000139 DACDIP010000000
VERSION     DACDIP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.142"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            61..1521
                     /gene="gtfA"
                     /locus_tag="IV257_002775"
     CDS             61..1521
                     /gene="gtfA"
                     /locus_tag="IV257_002775"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8815832.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            1598..2704
                     /locus_tag="IV257_002776"
                     /pseudo
     CDS             1598..2704
                     /locus_tag="IV257_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         1025 a          495 c          555 g          901 t
ORIGIN      
        1 tcgaaaattg cgtttacgac cgtttgaaag tattgtatta gctaattatg gaggacaatt
       61 atgaaaatta aaaacgaagc aatgctcatc acctatgcag atagtttagg aaaaaacatg
      121 ggcgaattaa aacaagtctt agacaaacat ttacaaggtg ttattggtgg tgtacatctg
      181 ttgccttttt ttccatcgac aggtgatcga ggctttgcgc cgagtgatta tacgacagtt
      241 gatccagctc ttggaacatg ggaagatgtg gaagcattag gtgaagatta ttatttgatg
      301 tttgatttta tgattaatca catttctcgt gaatcaaaat ttttccaaga tttcaaaaaa
      361 aaccatgagc agtcaccata caaagaaatg tttatccgta ttcatgaatt tttcccagaa
      421 aatcgaccaa cacaagaaga tattgattta atctacaagc gtaaagacaa agcgccgttt
      481 caaacggtag aatttgcgga tggtacgaca gaagaggttt ggaatacctt tggggaagaa
      541 caaattgatt tagatgtgac caaagaagtt gtgaaagaat ttattcgaga aaccatcaaa
      601 gatatggcga accatggctg ttctttaatt cgattagatg cgtttgctta tgcgattaaa
      661 aaattagata ccaatgattt cttcgtggaa ccagatattt gggaactttt agacgaagtg
      721 cgtcaagaag cagcgaaata tgacgtcgaa ttattaccag aaatccatga gcattattcc
      781 attcaaatga aaattgccaa tcatgattat tatatctatg acttcgcctt gccaatggtc
      841 actctgtatt cactttatag tggaaaatca gaacgtttag ccaattggtt gaaaatgagt
      901 ccgatgaagc agtttacaac attggatacg catgatggaa ttggtgttgt ggacgctcgt
      961 gatttattaa cagatgaaga attggattac acctctgagg aattgtataa agtcggggcg
     1021 aatgtgaaga aggtctattc aagtgccaat tacaataatt tagatattta tcaaattaac
     1081 agcacctatt atagtgcatt aggcgataat gaccgtagct atctgttagc acgtgcgatt
     1141 caagtatttg cgccagggat tccacaaatt tattacgttg ggttattagc aggaaaaaat
     1201 gatattgaac tgttggaagc aacgaaagaa gggcgtaata ttaatcgtca ttactatgat
     1261 ttagccgaaa ttgaagaaga agtgcaacgg ccagtgattc aacaattatt tgcactttta
     1321 aaatttagaa atgaatctgc ggcgtttgat ttggatggaa cgattgaagt agcaacgcca
     1381 tcagaatcag aaatagtgat tacacgtaaa aataaaaacg gtagtgatag tgcaacatta
     1441 acggctaact tggatacgaa agaatttatc attgagtcta aagatataaa aattttcact
     1501 agtgttgcat taaaatttta acctattcca tagaacaaaa aatcctttat actagcactt
     1561 ggatgactta tccaagacca ataaaaagga ctcatacatg gataagtata aaacaaatac
     1621 agcatttaat aaatggtttt ctgccataaa attaggaaaa ctttctgtca actcccaaaa
     1681 atgtattttt gattacaatt cgtatcgtaa aaaactgtct tttgaagctg tgctcaagct
     1741 ctttctctac gcagttaatg aagaaaaaga aagtttgcgt gatttgagta cagctcttat
     1801 cgaccgatcg ctccaactag aaaccgatgt gtcatcgatt agtcataccc aattgtctcg
     1861 agcatttaat gcacttgaac ctaaagtgtt agaagaaatt tttcaacaac tgttagaaaa
     1921 agttcaacat caaatcaaac caaccaagcg aaatcatctg tatctgattg attccagtac
     1981 gttttcgctt agtttaaaac gtcataagtg ggcgactttc cgtcaaacaa aatcaggtgt
     2041 caagttacac ttaaactatt gttatatgga cgatactacg atgtatccaa ctgattttac
     2101 cttaacaaat gcccatgaac atgatctcaa tcaactacca gtattggtga atcaaccaga
     2161 ggcaacttac gtgtttgatc gtgggtatta ggactttgaa aagatggatc agatgcattg
     2221 ggatggctat ttcttcgtca ctcggattaa aaaaaacacc aaagttcacg tgatagatac
     2281 tctggaaaca tcgccagaaa cagaaattct acgagacgag ctggttcgtt taggatctaa
     2341 aacctatcta accgccaatt tccgactagt cactgttcaa gataagaacg gacgtgtgtt
     2401 tcaatttatc accaatcgaa tggacgtctc ttcaaaagaa atctctgata tgtatcacgc
     2461 tcgttggcaa atcgaattgt ttttcaagca catcaaacaa cacatgacca ttaaaacctt
     2521 cttttctcaa agtgaaaaag gcgtgcagaa tcaattgatt ttaacgatga tttccgctct
     2581 cctcacgttt ctcatcaaat tggagacgaa aacggaaaaa tcagtgttcc aaatcaaacg
     2641 attctttcgt tatttactgt ttcagccctt tgaatgttgt ctagaaaaat taattcccac
     2701 atgagaaaaa agaaatttgg ctcgttactt ttggttttca gttgaataac agataaaaaa
     2761 ttttctggaa aaagtcatcc tctgcgtcag cacagttttt tttgacttat tttcagagga
     2821 aatagttttc agaatattct aaaatgtttt atgcaacact agtgatattt tttagaatta
     2881 gatttctaat gtgcattttt tgagtattaa ggagcaagta tacaaagatt tataaagctg
     2941 attaaacttg aataaaaata tgcaggaacg ctacta
//
LOCUS       DACDIP010000140         2948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDIP010000140 DACDIP010000000
VERSION     DACDIP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.143"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..722)
                     /locus_tag="IV257_002777"
     CDS             complement(<1..722)
                     /locus_tag="IV257_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8815833.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALL"
     gene            complement(842..1489)
                     /gene="eda"
                     /locus_tag="IV257_002778"
     CDS             complement(842..1489)
                     /gene="eda"
                     /locus_tag="IV257_002778"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8815834.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1573..>2948)
                     /gene="uxaC"
                     /locus_tag="IV257_002779"
     CDS             complement(1573..>2948)
                     /gene="uxaC"
                     /locus_tag="IV257_002779"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8815835.1"
                     /translation="HKFAKHQPIIDYHCHLDPAYLLENKQYKNLTQVWLNDKGAGDHY
                     KWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPIFEWSHLELQRYFDIDLTI
                     SEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDDPTDDLWYHTELSKQDNSF
                     KVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGLKQALSQRVDHFHQIGCRL
                     ADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQFVTGIQLHLMSEYTRYGWT
                     MQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVALFDTAEKSGCLPKTILYS
                     LNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMRQQLTTMAQQSLLSNFTGM
                     LTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYLSQIVENICYRNAYNYFNF
                     FEIGSRK"
BASE COUNT          934 a          614 c          404 g          996 t
ORIGIN      
        1 agtaatgccc tatttttaac taaccctttt aaagatttta acaatgaaac tttgtattga
       61 gattggtcaa ttcttactcg ttcagtagtt agtttataac atattaaata acaaataagt
      121 gcacatatcg aaaatattcc cgcaacaatt gtaaaagttg atccaccacg aactatttga
      181 gcgcctgaat catctcttgt ataaataaat aatggtgtta atacaccaat gaccaaactt
      241 gctaaagtag ctccaacatt tcggaatgtc gatagcattg ttctttcttc tgtaatagaa
      301 gtaatagctg atgccattga tccataagga atatttatag atgtgtaaaa aatactcccc
      361 cataatatat acgtaacata catgtatact accttaacgc tcatagaagc tgaaatcata
      421 gaagtttgat acattaaaaa acttgctaaa gcgactggac cactcattct taaaatccac
      481 ggtttaaact taccctgctt agttgctttc gatctatcaa caatacgccc catagttaca
      541 tcagtaactg catcgataaa tcttgctact aaaaataatg ttccaacggc agctccgctt
      601 atccccaata cttttgtata aaagatcatt aaaaaagaac tagcaaaaat gaaagtaaaa
      661 tcgtttccaa aatctccaaa catatatcct attttgtctc ttataccaaa ttttctttcc
      721 atacaattct ctcccttctg ttgttaagcg ttttcattct gatgaaagta cgtactatac
      781 tctgaagcgt aaagagtcag tatttatttt ttacatttca gagtattatt tgttttttct
      841 cctaataatt ctttatctca taaaacttat ctatatattt actagctact cgacttactt
      901 cttcaaaatc acctttttca acagaagcca ataaattccc acctacacca actgcaacaa
      961 ctcctacttc aaaccaatct tctaagttct ctatacttac tccgccagta ggcattatat
     1021 ttacgtgtgg taatggagct tgataggctt ttacaatgga tggaccaaaa gcagaacctg
     1081 ggaatagttt aatgatatct acgccagcag taagtgcctc ctttatctca gtaattgtca
     1141 tacaacctgg taaataagga atttgatata aattacaaat ctcagctgtt tgtcgatcaa
     1201 aagatggact aacgatatat tctgccccag ccataattgc gatacgagca gtaaacgcat
     1261 ctaaaaccgt accggctccg attattacat ctttttgatc agcatatttt ttagcaattt
     1321 ctttgattat ttcttcagca ttaggaactg taaaagttag ttcaatccct ttaattccac
     1381 ccgcaataat tgaatttatt gctttaatgg cctcatcctt atttttccca cgtacaaccg
     1441 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
     1501 tcctttatgg atacatactt tgtctagctg tactaactta caaagaataa atttaagctt
     1561 ttatttcaag tattattttc tacttccgat ttcaaaaaaa ttaaaataat tatacgcatt
     1621 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcggcc
     1681 tgctttaacc cactcgctta gtaattgaca taatactcga cgaaaatact catggcgagg
     1741 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
     1801 ttgtgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
     1861 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
     1921 tggaagccaa tcagttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
     1981 ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
     2041 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
     2101 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
     2161 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
     2221 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
     2281 accgtgatct gctaatcgac agccaatttg atgaaaatga tccactcgtt ggctcaatgc
     2341 ttgcttcaac ccattaaagt ctttgattgg tatagccgct gtttgactaa gttttaaaat
     2401 atagttcgaa aagcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
     2461 taccttgaaa gaattatcct gtttagaaag ctcagtatga taccacaagt catctgtagg
     2521 atcatccgtt gtacagatgg ctttcacatc catcatctcc aacaattctt tcgggcgata
     2581 ggaatccgtt tgaatttttt cgttagcacg tcgccaaatt tctggtgcat tcttctcaga
     2641 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
     2701 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcatcacc
     2761 tggaccactg atcaactctt cttctatacc attagctcgc atcaatcgcc atttataatg
     2821 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
     2881 tagcaaatat gctgggtcta aatgacaatg atagtcgata attggttggt gttttgcaaa
     2941 tttatgaa
//
LOCUS       DACDIP010000141         2737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDIP010000141 DACDIP010000000
VERSION     DACDIP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.144"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV257_002780"
     CDS             complement(<1..132)
                     /locus_tag="IV257_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8815836.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     "
     gene            207..485
                     /locus_tag="IV257_002781"
                     /pseudo
     CDS             207..485
                     /locus_tag="IV257_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
     gene            628..849
                     /locus_tag="IV257_002782"
     CDS             628..849
                     /locus_tag="IV257_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8815837.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            865..1707
                     /locus_tag="IV257_002783"
     CDS             865..1707
                     /locus_tag="IV257_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815838.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1770..>2737
                     /locus_tag="IV257_002784"
     CDS             1770..>2737
                     /locus_tag="IV257_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HAP8815839.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSP"
BASE COUNT          970 a          400 c          543 g          824 t
ORIGIN      
        1 atgagaaacg ttaatgccac gatcatataa gatttcttga acttcacgat agctaaggtt
       61 ataacgaaga tagtagccca cggctacaat aatcacatcc tgctgaaatt gctttccttt
      121 aaaatgattc atcgtcattc ctcctgctat ctttttctat tattctacct tatttgatag
      181 tagatttaaa actttgcaac agaaccaaag aactcatcaa gctggctaat aagggtcaag
      241 attatgaaca tctagcagat gagattgatg agctgagaga caagcgacag ctccttttag
      301 tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt gaatttatcc
      361 gcaagaacaa attccgtacc ttagagtacg atgataagct tgtaaggaag ataatccaga
      421 gcgttacagt ctatgaagaa cacttcgtca tatccttcaa atctggcatc gaaatggaaa
      481 tatgaaaacc agaagtaaat agcccatgat tctacagtag agtcgtgggt tttcttttgc
      541 ttgctgatta taataatatt ttaataacta ttgttattat aaaccaaatg atttataata
      601 ttcttagcgt aaacttggag gtgctgtatg actacggcag aaatgattaa agaactgtgt
      661 gagcaaatga atataagtgt ttccgaactt gctagacgta ttggacagac tccacagaat
      721 ttcaataaga aattacaacg agaaacggta accttagatg agttgaaagc gattgctgat
      781 gtgctaggtg tcaagtttgt gcaggctttt atattgcctg atggcaatga aataaagata
      841 ggtaattagg aggtgtaatt tgtattgaaa atcataacaa taaatgatgt tgagtatgct
      901 gtatttgctg ctaatgaagg tacttccaaa ccacaacctc atatcataga aacgaaatct
      961 ggtactattc cagaaggaaa acaactgagt ttattaaaag aatatcttaa gcagaatgat
     1021 atttccccaa taaaaggagc aacaacatat tggtgcattg acaaggttct taagcttgat
     1081 tcaagtaaag aaaaaactat aagtgaaaca atccataaac aaaaatactt atctttaact
     1141 gaagaaaata ttgagaaaca gcataagttt gtaggtgcaa gctctaatta tgggaaagaa
     1201 ggtctaataa tacatgatgt tttgaatgca tttcctcttc ataatgattt aaatactatt
     1261 gcgatgaaga ttgcggtaat tgatgtaaca aattcaacac atctttctca atataagagc
     1321 aggttgtctc tatatgattt ggctaaagta attctggaga ttccaaattt tgatgatcgg
     1381 ttggcaaaag gagatcctca gttgataaat attatcgcaa ggaacattgg tgcagttaat
     1441 atgttctcat ttgcatccaa gtactgtaca tatcataatg tggaagtgtg tgggagagat
     1501 gattactcca tctttgatgg tatcgttaaa aatactcttc cacactatat ccaaggacta
     1561 actacgaata agattgatac gtggcgaaga agttttgatt acgaggcatt taacgaatgt
     1621 gttggaaaac tacttgatga aaataatata catattcctt ttagaagaag aaagttagat
     1681 cacttcttgt ggtatgcgaa taggtagaat ataagaaaaa ggcatcgtaa aagtgattct
     1741 ggataaaatt aatgggggag gaaataatag tgggattatt tgattttcta aagaaaaaac
     1801 aaggaaaggt tagtaaacac gttgattatc aactggcttc tagtctattt cgtactggta
     1861 aagtttttgc tgaaaaattg gaagtggaca tatcaaatgt agatgagttt cgtaaaactt
     1921 ttatcgcttt tgatgtggaa acaactggcc taaattcttc ttcagataga attgtagaga
     1981 ttggtgcggt gctatttaaa gaaggagttc caattcaatc attcggaact cttgtcaatc
     2041 caaaagttag aattgcagcc tcagcttcgg caataaataa tataactaat gaaatgctaa
     2101 actctgctcc aaatgagcag gaagtttacc cccagttggt agaattttta ggagatgcac
     2161 tagatggaaa aatcactatg tgtgcacaca atgcaagttt cgattttgga tttttaagta
     2221 acacattttc tagacttggg tatgatgcta aaattaagta tgttgatact ctgagtctct
     2281 ctagaaaaca tataaaaggc attgtaaatc ataagcaaga gactgtagcg gaatatttcg
     2341 gattaataaa tgacactgcc catagagcgg aatcagatgc agatatatgc ggaaagattt
     2401 tttggggtgt tttagatatt attgaggagt cgttggaaga acaaaaaaag caaatagaaa
     2461 agatgactcc agacataaaa gaacttgaag cttgtgcttt tattcaagat attattgaga
     2521 aaaaaggatg cgacaccagt tggatcaggt atcgaaaaaa cagtaataat tatgtcgatg
     2581 taacttgtct atatacattc ttaaagttta agtttgctaa gaagggaaat tacatcatta
     2641 ttagaaaaga tgctacaaat ggaattgatt tacctataga accttgtact gtaagtgaag
     2701 gcggtacaac aaatgtgcga gtctattttc gtagccc
//
LOCUS       DACDIP010000142         2664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDIP010000142 DACDIP010000000
VERSION     DACDIP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.146"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..305
                     /locus_tag="IV257_002785"
     CDS             9..305
                     /locus_tag="IV257_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815840.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            307..729
                     /locus_tag="IV257_002786"
     CDS             307..729
                     /locus_tag="IV257_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815841.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            748..2340
                     /locus_tag="IV257_002787"
     CDS             748..2340
                     /locus_tag="IV257_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815842.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2361..>2664
                     /locus_tag="IV257_002788"
     CDS             2361..>2664
                     /locus_tag="IV257_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815843.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         1076 a          388 c          522 g          678 t
ORIGIN      
        1 ggatatttat gaatgttaaa attagagatt tagatccaaa attcatttct gaaattgatc
       61 gaagatgcgt tgaactaagt aatcgtacag gaaataaatg gagtcgtaat gattatttaa
      121 agctactcgt tgaaaatgat tttgatcgtc ctttgatgga atacaaacaa gaaaagtttg
      181 accagttatt agaaaagttt tcagaaattc aaagttataa cacaaagata ttagaagaat
      241 atgttagcca aaataatcgt attatagaaa tcttgattga gcaaaataga ggaagtgaac
      301 tttgatatgg caaaggttat agacaaacac atttactttc accaaacaca tatcgatgta
      361 ttggatgaaa ctatttctac tcatgcagag gttaaaaact atagtgaagc tgttcgcttt
      421 ttatgtatga actatgatcc agaagtggag aaaaaagtaa attccaatca agcaaagttg
      481 aatggaatga gtaaagaaat ttctatttta accgaaatgg tttcagaatt tgcggatctt
      541 catttgaaag atacaggaat attaattggt aaaaattcaa ctgtttatca agaagcaaaa
      601 gaacgagtgg aaggaaaaat taaatccaat caaacaaaaa aatattcccc tcagttgaag
      661 aaacctgtga atgaatctgt tcatgccaga gtagatccat ttgatccaac gaagattcta
      721 aaaagataaa aatgaaggag gataataatg actcaatcgc cttcagttat tctcacttgt
      781 caatttactt tgccgaataa aaagtcattt tctacttata ttgactatat tacaagggaa
      841 caggctcttg aagaaaagga acgacgtacg cccgaagaag aacaggaact tcaacgaatc
      901 aagaatgctt tagaggagtt ttatattcct gaaggagaaa ctttttcgga gagtaagacg
      961 ggtaaagaat tcagtgaaac gtcgaatgaa gctcaatcgt tgatggaaca tggaatcaat
     1021 tttgacaagt tagaggaaca agattttacc aattatcttt cttatatgac acgtcactat
     1081 gcgttagaac aaaagaaaga actgacgcaa agtgaacaaa aagaatacca acgcttgaac
     1141 caagcaatga aaaaatatca aacagaaccc cataaaaaag cttcaggaaa tcttcctgga
     1201 gttttttcta tagcttcaga cgaagtgaaa ggaagtgatc tgaaggagat taagcaaatc
     1261 ttccaaagag gacaacaaaa aggctcaatc atttatcaag atgttgtttc tcatgataat
     1321 tcgtatcttg aaaaaatagg attgtacgat cccaaaacag atactttaga tgaagaaggg
     1381 ttaaaagcag ctggaagaaa aatgatggag acattattcg agaaagaaca attgaatgaa
     1441 acaggttact ggatggcaac gattcaccgt aacactaaac atatccatat tcattttgca
     1501 gttgttgaaa aagaaaatac gcgtaaacct tacacggagg ttgaaaatgg tgtgagttat
     1561 atcgtaccga aaggtaagag aagacaagca acattagacg caatgaagac caattatgta
     1621 catgagttag aacgtttttc ttatgaaaag gatcgaatga ttggtatgga gaaacgaaat
     1681 ttattgacgc ggaaaagtga tttgcgtaac accttaacaa aagtggtaaa agagccagcg
     1741 cgttacgatc gacacgcgat gaacttatta aatgaagtat ataaaaccct acctgacaaa
     1801 cgttcggaat ggaattacgg ggatgaaaaa cgaaccaaac tttcaccata cacaagagag
     1861 aaactcgatg agttaaccaa atatgtttta acaaaagaac ctgaatatca agaatatata
     1921 cagatttcaa aagcattgaa agaagaagca aaaagtcttt atggggaaag caagagagaa
     1981 tcaaaggatg cagagaaaaa tgctctcttt gatttaaaaa agcgcacagg aaatgccatc
     2041 ctaacagagc tgaagagaca ggatagtcaa ataaaaccat taatagaaag tgttgatcag
     2101 atgcaatcta tttacaatga acgatccttt cgaacgctta aacaattgcc aacgaatgaa
     2161 tacaaatata gttacgaaga attcatgaag cgcaggaaac aattccaaca aagagttgtc
     2221 acgcagcgga gtttgcgtca attaaatcga acgattaatg cccatgaaga aaaataccac
     2281 gcaagtaaag catacgaaga gcaacaacga agaattcaaa tggaacaaga aagacagtaa
     2341 agacagtagg aggaatagca atgaaacaat atttagatca atggaaagtt atagaaggat
     2401 cattaagaga agaacgaatc gaacaacttc ctgattgttt agaaaaagag catttgttcc
     2461 agataagaga aatgttgaga aacgaacagt ttgatccaaa tcaattttta gtcgttgagt
     2521 acccagctac tggtgtgtat tgttgtaatc atgtgaatgg tgaaaaatat tttatcattc
     2581 aagaatatga gggcaagtta gcaccttatt atactacttg ggaaatgaat gaagagggaa
     2641 tcaataactt tccatgtaaa agca
//
LOCUS       DACDIP010000143         2579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDIP010000143 DACDIP010000000
VERSION     DACDIP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.147"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..622)
                     /locus_tag="IV257_002789"
     CDS             complement(<1..622)
                     /locus_tag="IV257_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8815844.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRS"
     gene            complement(971..2422)
                     /locus_tag="IV257_002790"
     CDS             complement(971..2422)
                     /locus_tag="IV257_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8815845.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT          742 a          497 c          367 g          973 t
ORIGIN      
        1 tagaacgata aactccctta acaccacttg tattattttt cggtaatttt gacgttagac
       61 actctattac tgtcccttca acacatgtcc ttgtttcttg cctttttaaa catccacaac
      121 tttgagtatg tccaaaaaat aagttactat aacttatctc acacatactg ccacactcac
      181 attgacaaac ccattttgtt ttccatgaac agttattgtc caaaggatac tttgcagtaa
      241 gtaatccgaa ttttttattt tcaatgtgat gcttttttga tttcaaacat ccacagcttt
      301 ttacatcacc ttttagaagt cgctttgccg atactaactt ttttctccca cacagacatt
      361 gacattccca aacaacagtt ccttcacttc gcagtttagt tggcttgatt gcaactaact
      421 taccaaaagt tttatttgtc aaatttttat atttatgcct atttttataa tttccacatc
      481 cacaactttt ggttctacct gatagtaagt ttgatcgata tacaatcgtt tcattcccac
      541 agtcacatag gtaacgatat tttttatatc cattatgagt aatctcctca acaacagtaa
      601 ggtgaccata tctttccttc actgcaccta ccctcccaag aaagaagtac catataattt
      661 caattaactt ttaataaatc agaaaaaaat attttcaatt tttcttagtc tgattttatg
      721 actaatttat aatacaagac tgaacgatac gtacacgaaa tcctcaagat agataaattg
      781 ttttcatcta gtaaaacctg ctcaaaatat gagcataaag acggcaataa tacactcgat
      841 attcgataat ggacttgttt tatttgtcaa actcatcgta atcgagatat caattccagt
      901 ctcaatatcc tgatcaaagg tctaagaatc caaccatttt cttgttactt tgtcagattg
      961 attacctttt ttagtctccg aataataagg ttttcggatc aactaacatg ctctgagaaa
     1021 gactactatc tttgcaaatt tccaaatgaa ggtgtgggcc agtactattc ccagtacttc
     1081 ctacatagcc aattatttgt ccttgcaaaa cattatctcc agtttttact aggtattgtt
     1141 gctggtgagc atataacgcg gttgatccat cttcgtgttg tatcaccacg taatttcccc
     1201 aagagtagtg aaactctgct ttaacaacct taccagcttt aactgcatga atcggctcac
     1261 cctgtgtagc tgccatatca agtcctcggt gaaattcatt tccacgattt ccaaacaaac
     1321 tggttattga ataattatga ataggcactt ggtatccagt cttactcatt tgtggacctt
     1381 taacttcttt atcatagttg gttaaatcat aggttttaat taattcattt agcttcaaat
     1441 agtattgtgt atctgttgca taccgtcctg taaggaattt agttgcttct cgatatgttt
     1501 tactattagc ttttaatgct cctgcataaa aatctttatt tccactaatt cccttagtta
     1561 ataatttggc ataatcttca aaactttcct catagttttc atacactcta aattttgatt
     1621 gggtcgtata aagcgttcca ttacccaaat cttcgtttgt taccataatg acaaagtttc
     1681 cattataagt tccttttatc ccaaataaat tgtaattcgg tgcctgagct aactggcttt
     1741 gaccactggc agattctaag atcgcttgtg caatcataac agaagcatat aaatcatatt
     1801 tctgaccaat ttttctcgca gactcaccaa ttttcataat aaaattttct acagattcat
     1861 ttttgtcaaa atgaatttct tcattaccag atagtattgg ttgtgattgg aaagattctg
     1921 ttgattctat ttgctgtggt gtcgaaaaat tttttgtttg tccttccgtt tttggcgctt
     1981 ccttttttga agactctata tggtttggtc ttgtcacttt cgaagaagtt atttcacttg
     2041 tactacttga agttgttgaa gattcggcac ttgaaatcgt actactttca cttgacgaag
     2101 ttgttgaaga ctcggcattt gaagtcgtac tactttcact tgacgaagtt gttgaagact
     2161 cggcatttga agtcgtacta ctttcacttg atgaagttgt tgaagactca gcatttgaag
     2221 tcgtactact ttcacttgat aaagttgtca aaagttcact actctctgct gttacatcgg
     2281 aattactcat ttcagtcgtc tcggttcttt cttttgcaat tttgtatgtt gaattttcgc
     2341 ttgccaacac atggaaaggt gttgctataa agagttggga cattaatata actgctgatg
     2401 caagttttaa agattttttc atttgattta cctctctttt ttctctttaa tagtgttttc
     2461 tattaaaaat tatgataaat attttcaatg aattgagtaa tacagtatat ttcttgattt
     2521 catttagtaa cgattatgtt tcaatgttta atcctgttac tgcctttaaa ataaaatta
//
LOCUS       DACDIP010000144         2485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDIP010000144 DACDIP010000000
VERSION     DACDIP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.148"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1016..1306)
                     /locus_tag="IV257_002791"
     CDS             complement(1016..1306)
                     /locus_tag="IV257_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815846.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1514..2205)
                     /locus_tag="IV257_002792"
                     /pseudo
     CDS             complement(1514..2205)
                     /locus_tag="IV257_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          735 a          459 c          348 g          943 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat ttaggtttac
     1501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     1561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     1621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgattt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg taattttaat ataaatacta tcaaatactc atttaatcct ccttttttat
     2341 ctatttaaaa aatctttttt atagagaaaa atgcaggtta atactttctt aaataaatat
     2401 ttatggtgaa caaaaacaaa aaagcaagtt ttattaaacg taaccctttt catttcatag
     2461 attactgtat ttattacaaa aaacg
//
LOCUS       DACDIP010000145         2465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDIP010000145 DACDIP010000000
VERSION     DACDIP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.149"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..97
                     /locus_tag="IV257_002793"
     CDS             <1..97
                     /locus_tag="IV257_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8815847.1"
                     /translation="ILGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(127..1542)
                     /locus_tag="IV257_002794"
     CDS             complement(127..1542)
                     /locus_tag="IV257_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8815848.1"
                     /translation="MSNKEEAKSIINFVGGKENIAALTHCVTRLRFALRDDTKVDKAG
                     LENLEDVKGTFNANGQFQVVIGPKVADIYEEVISQSGLSGANKEDVKKVAAELNLNWF
                     QRAVKILGDVFVPILPAIVTAGLLMGLNNLLTNPGIFFEQSLIEVYPNIADVADMINL
                     IANTSFQFLPALITWSTVKYFKGNPLLGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHW
                     TIFGLSISKIGLQAQVLPALVSGYVLAKTELFLKKRVPETIQLLVVAPVAILFTSVIT
                     FIIIAPITIAIGNTIVAGIVWLFQKVPFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLM
                     ANGGDYIWPIGVMSSLAQGSAAIAMNFLIQNKKLKGVGLAAGLSAYMGITEPAMFGVN
                     LKYKFPFVVSILTSGIIGGFMASQHVIATSVGISGIPGFLSIPFEFWPIFFICMLASC
                     VVSFGATYLYGKNKVLLQSFK"
     gene            complement(1535..2089)
                     /locus_tag="IV257_002795"
     CDS             complement(1535..2089)
                     /locus_tag="IV257_002795"
                     /inference="COORDINATES: protein motif:HMM:NF025668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815849.1"
                     /translation="MDVLCGKINPAGKLTITIPRHLGQIPIYYNYFNTGRPYSSEKFN
                     TTKYLDVSNEPQFPFGYGLSYTTFSMSPISIKEKGKNCLKVTTTVSNKGSRDGYEVVQ
                     LYIGAQYGRYIRPTKDLVAYKKVFVKSGDSVTVSFELTNKDFKYYDEEFNEIIDDGAY
                     WIMVGNDSSQALTNKEEIEVRINE"
BASE COUNT          819 a          511 c          387 g          748 t
ORIGIN      
        1 cattctagga ttatataaga aaacccgaaa agaaggcact ctcttcgggt tttcggtctg
       61 tactgaaatc aaggtattat tgggaatccc agcttaaatc atagataccg taagggattt
      121 tattctttat ttaaaacttt gcaacagaac cttatttttc ccgtaaaggt aagttgctcc
      181 aaatgagacg acacaacttg ctagcataca aataaagaat attggccaga attcaaacgg
      241 aatagacaag aaaccaggaa ttccactgat accaacagaa gttgcaatga cgtgttgtga
      301 cgccataaat cctccaatta ttccagaggt cagaatggaa accacgaatg ggaatttata
      361 ctttagatta accccgaaca ttgctggctc agtgataccc atataagcag acaaaccggc
      421 agccagtcca acacctttca actttttatt ttggattaag aaattcattg caatagcagc
      481 agaaccttgt gccaaacttg acatgactcc aataggccaa atataatctc caccgttcgc
      541 catcaattga atatgaactg gtaaaaacgc atgatgcatc cctgtcacga ctaatggcgc
      601 aagaccaata ccataagcaa atccgccaat aaatggaact ttttggaata accatacaat
      661 acctgcgaca atcgtattac caatagcaat tgtaatcggc gctataataa taaatgttat
      721 aacactagta aaaagaattg caactggtgc aacaaccaac agttggattg tttcaggcac
      781 acgctttttc aagaatagct ctgttttagc taacacatag ccggaaacta gagctggcaa
      841 tacttgtgct tgtagtccta tcttagagat ggacaatcca aagattgtcc aatgtggaat
      901 atctcccgtg gttactgctt gcccgtaatt ccaggcattc atcaaatctg ggtgtacaaa
      961 gactagacct aacacaatcc caagaagagg atttccttta aagtatttta cagttgacca
     1021 tgttattaac gcaggcagaa attgaaagga agtgtttgcg ataagattta tcatatcagc
     1081 gacatcagca atattaggat atacttcaat gagtgactgc tcaaaaaaga ttcctgggtt
     1141 tgttaataaa ttattcagtc ccatcagtaa accagctgta acgatcgctg gtaaaatcgg
     1201 cacaaataca tcacccaata ttttaactgc acgttgaaac caattcaaat tcaactcagc
     1261 cgctactttt ttaacgtctt ctttgtttgc cccactcagc ccagattggc ttataacctc
     1321 ttcatagata tctgctactt taggaccgat aacaacttga aactgtccat tagcattgaa
     1381 ggtacccttc acatcttcca ggttttccaa tcctgcctta tcaactttag tgtcatctcg
     1441 caaggcaaac ctcagacgtg tcacacagtg agtcagtgct gcaatattct cttttccacc
     1501 aacaaagtta atgattgatt tagcttcttc tttattactc attaattctt acctctattt
     1561 cttctttatt agttaatgct tgagatgaat cattgcctac catgatccaa taagcaccat
     1621 catctataat ttcgttgaac tcttcatcat aatacttaaa atctttgttt gtaagttcaa
     1681 aagaaacagt cacactatcc cctgacttaa caaaaacctt cttgtaggct accaagtctt
     1741 ttgttggtct tatataacga ccatattgag ctccaatata tagttgaaca acctcataac
     1801 catctctact tcctttattt gaaacggtag tagtcacctt caaacagttc ttacccttct
     1861 ctttaatgct aattggtgac atagaaaaag tggtataact caacccatac ccaaagggaa
     1921 attgtggttc attggataca tctaagtatt tggtcgtatt aaacttttca cttgaatacg
     1981 gtctaccagt attgaaataa ttataataaa tagggatttg tcctaaatgt ctcggaattg
     2041 ttattgtaag ctttccggct gggtttattt tgccacaaag gacgtccaaa atagcattgc
     2101 cagcctctgc tcctagatgc caagcctcaa ctaatccctt acatatagat ttttaaatta
     2161 cacactagtt taaactaaaa caaacaagga aaaacttacc atttaatcaa aaaattagct
     2221 catatgaata tattagaatg taaattagac tggtcaaaat ctggtcaaaa aagaggacga
     2281 atacaggtag tcctcttttt tgttattttg atattctaaa tcatttttta ttggattatt
     2341 cccttgttcg atatagaccc aaattgatct atcaataatt atcaaactga gtcatataga
     2401 gacaaaaatt tcaaaaatga cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg
     2461 acgga
//
LOCUS       DACDIP010000146         2462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDIP010000146 DACDIP010000000
VERSION     DACDIP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.150"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..277
                     /locus_tag="IV257_002796"
     CDS             <1..277
                     /locus_tag="IV257_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8815850.1"
                     /translation="PTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPVLIRATMI
                     AEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
     gene            279..1703
                     /locus_tag="IV257_002797"
     CDS             279..1703
                     /locus_tag="IV257_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8815851.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            1721..2290
                     /locus_tag="IV257_002798"
     CDS             1721..2290
                     /locus_tag="IV257_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8815852.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT          876 a          312 c          451 g          823 t
ORIGIN      
        1 tccgacttat cgttctgaaa taagaatgat taaagaatct ttaaaaatga gaaacattcg
       61 cttgtctttt tttcagcctc tgcaatattt ggaatttctg ttagttcccg ttcttatacg
      121 tgcaactatg attgctgaag aaatgaccgc taatgcaata acaaagggga tagaatctcc
      181 tgtgaaaaga agttcttact ataaaacaaa catgagctta ttagactata tcatcctgtt
      241 ttccgttact atttttttct taagtatttt gatttgaggt gaaagaattg tttgaattta
      301 acaatgtttc tttttcatat aaggatgtta aggaacaagc gcttgttgac cttaattttt
      361 caataaaaaa aggtagtctt acattgatta ctggttcttc tgggagtggg aaatccacac
      421 tattaaagtt aatgaatgga cttattccaa gactttatga cggaaaattg gttggggaaa
      481 ttttatttga aaataactcg ctacaatcct taacacaaga aagtatatca aaaaatattg
      541 gctacgtaag tcaagatcct agaggacaat tctttacaac aaatacaacg agtgagttag
      601 tattttcgat ggaaaacttt ggatttacaa aagagcatat ggataaaaga atcaatgaat
      661 tagctgaatt attagatctt tcaaatatat tagataaaaa cattttttcg ctatcaagtg
      721 gtgaacggca gaaaatttct attggatgta gtttatcgct aaactctaaa ttaattatat
      781 tggatgaacc gtcttcaaat ttggacttca aagcgactaa gaaacttgct gacttacttt
      841 gtgatttaaa aaataaagga tttactattg ttatagctga acatcgtttt tactatttga
      901 aagaattaat tgatcaagtt ctatttgtta aaaatagaac tgtaaaaact acaactattc
      961 agttgttaaa aactgaaaac aataacgcat taaggaaatt tgacattttt aatagttcga
     1021 ttgaagttcc ctatatcgaa aaggtaagtg cagatgtatt aaaagttgaa gaaattactt
     1081 atgaaaatat tctaaaagac ataaattttg atgtcaaagt tggagatgtt attggtttgg
     1141 tagggaaaaa cggtgtgggg aaaacaacct tactaagaat actcatgaag attattcgcc
     1201 ctaccaaagg aaagataatc gaaaataaaa aaaatttgtc atcgccgttt ttagtaatgc
     1261 aagaaatgga ttaccaattt ttcacagaat cagtgaggag cgagttagcc ctggggaatg
     1321 aaatagttag taaagatcag caagaaaaaa ttttaaaaaa aatggaactt tataaaatga
     1381 aagatcaatt accgtttgat ttatccggtg gagaaaaaca aagattattg gtttctatag
     1441 cttcattgtc aaaaactaat ttatttctat ttgatgaacc tacaagtggg ttggattata
     1501 ttaatatgga aaggattgct gaccttatta aagacttaaa agaaaagggt gcagtagtta
     1561 tagccactca tgatccggaa tttctttata aaacatgcaa tagaattatt tatttaaaaa
     1621 atggtggaat aaaaaaagat ataaaactta gagtagaaga ttcaactttg atagaacaga
     1681 tttttaatga cataacgaaa tagaattgag gagaatatat atgaaaacaa aagattatat
     1741 ttttattggt ttagctacag ttatttccct agtaatttac tttttttcaa taatggttag
     1801 tagtattggt ggggcgtttg gtcacagcat atccccaggg atttttggat tattatctgg
     1861 aatcatattt gtttatatta gctataacta ccctagaaaa ggaattttta ctatttacac
     1921 aattgtttta ttgttatttt ttacgttaat gggcggggcg tacttacctt ggtttatcag
     1981 ttcgattagt atggcgatct tagctgatat tattctatct gtatttggat atgatcgagc
     2041 aattcctcaa gttgcgtctt gggctctaat gcaattaggc agtgctgccg gacagtggat
     2101 tcctatatgg ttctttacag atcgttttcg tcaagattgg atagataaag gacaatctgc
     2161 tgcaacaatg gatgcaatga tccactatgc ggtaggtata tggggaatca tttcagtgtt
     2221 agttgtagca agtttatcta tgattggtgt attgattgga agaaaagtct taaaaaaata
     2281 caaaaaatag aacgtagttt accttctatc tgtttgtaca tctctaattt acgctattac
     2341 ggaaagcatt aacagttgta aacgaataag agaagaaatt gaatgcgggg gaggaaaaaa
     2401 tgaaagtata caaaaaatta tttagttatg tccctaaaat gaaatactac ggatggttag
     2461 cc
//
LOCUS       DACDIP010000147         2402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDIP010000147 DACDIP010000000
VERSION     DACDIP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.151"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IV257_002799"
     CDS             207..2069
                     /locus_tag="IV257_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8815853.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..2401
                     /locus_tag="IV257_002800"
     CDS             2069..2401
                     /locus_tag="IV257_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8815854.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          716 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
     2401 at
//
LOCUS       DACDIP010000148         2371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDIP010000148 DACDIP010000000
VERSION     DACDIP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.152"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            155..1456
                     /locus_tag="IV257_002801"
     CDS             155..1456
                     /locus_tag="IV257_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8815855.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1560..1760)
                     /locus_tag="IV257_002802"
     CDS             complement(1560..1760)
                     /locus_tag="IV257_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8815856.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          769 a          454 c          408 g          740 t
ORIGIN      
        1 tttttcgtac aaaaaaaccg tcttttcttg gctctataat ttctaggact gatagaaaat
       61 cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc cgttagaatt
      121 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact
      181 actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa
      241 gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt
      301 gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat
      361 tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca
      421 ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt
      481 tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac
      541 gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct
      601 gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt
      661 tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg
      721 aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca
      781 atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta
      841 tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat
      901 tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga
      961 tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct
     1021 gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg
     1081 ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg
     1141 aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac
     1201 tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa
     1261 aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa
     1321 tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt
     1381 tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga
     1441 atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat ctcactctat
     1501 cagtcctatt tgacaaagag cccttttctt tcgaaaagac ggttaaaagt caatttttat
     1561 taagctttaa caatgtttgt tgcttgaggg ccacgttgac catcttcaac atcgtaagac
     1621 actgcttggc cttcttctaa agttttgaag ccgtcacctt ggatagctga aaaatgtgca
     1681 aatacatcat ttccatcttc accagtgata aagccaaaac ctttgtctgc gttgaaccat
     1741 tttactgtac cgttattcat aaataaattc ctcctgatac gtatcaatgt acgttttttc
     1801 gttgcaataa atatttgata ggcaaaagga gttttatcta tgtagatata tcgctcaaat
     1861 tacgtttcaa aatagattac tcatttaata tatcatgctt tttctaataa cacaaggata
     1921 atgcaatgaa atataaaatt cattgtcttc tattgtcttc gaatcgaaga aaaagttgaa
     1981 taaccgcgat tgattcactt tttgttttct tgtttatttg cttttatctg caacttttga
     2041 ctttaaaatt tcttaactat gaaaaccttt caattggtgg tatacttata aatggaattt
     2101 cgttatcaac gcgttcgttg cctttcagca agtatttaaa gaaaggagaa tggctatgac
     2161 attctataac aaagatatac aatatcgagt aaccttggat acagatttga atctatttgt
     2221 cgtttttgac aaagaaaata ccaatcatgt agcgacaggt gttacgatcg aacaagcagt
     2281 gcaagaatta aaaaaatctg cataaggagt ggaaaccctc atatggacga ttctcgttct
     2341 tctctttaaa cgcatctact ggtagataac a
//
LOCUS       DACDIP010000149         2352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDIP010000149 DACDIP010000000
VERSION     DACDIP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.153"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IV257_002803"
     CDS             <1..544
                     /locus_tag="IV257_002803"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8815857.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IV257_002804"
     CDS             complement(1017..1589)
                     /locus_tag="IV257_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8815858.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IV257_002805"
     CDS             complement(1605..2210)
                     /locus_tag="IV257_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8815859.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          469 c          359 g          784 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta gt
//
LOCUS       DACDIP010000150         2276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDIP010000150 DACDIP010000000
VERSION     DACDIP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.154"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..247
                     /locus_tag="IV257_002806"
     CDS             47..247
                     /locus_tag="IV257_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8815860.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            1001..2227
                     /locus_tag="IV257_002807"
     CDS             1001..2227
                     /locus_tag="IV257_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8815861.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT          812 a          327 c          479 g          658 t
ORIGIN      
        1 ttcttgcaag gttacttgca agaacttttt tggaggatat acatttatgg aacacggaac
       61 ggttaaatgg ttcaacaatg aaaaaggatt tgggtttatt acagtagacg gcggagatga
      121 cgtatttgtt catttctcag caattcaagg agacggcttc aagagcctag aagaaggtca
      181 agaagtagaa ttcacgattg ttgaaggtgc acgcggacct caagctgctg aagtgactaa
      241 attataataa tttgttgaat aaagacacga cttcttagag agtcgtgtct ttttgtctat
      301 ttatgaagtt tttagatttt ttggctaagt aaatgcattt gttcacttga catgtcctaa
      361 ctaattatca agcaaggaac attttcttcg ttagtaatgg attttaaaga aatttcagtt
      421 tttggatgtt gaataaaaga ccatttgtga aaccactagt atacatactc attaccatgt
      481 caattctatt actttaaaag aaaatcgttt tactttgaag aggagcatat ctatccatta
      541 aaagatgatg gtaatgcaat cactagttcg agtcatttta agagccatgc agtgagctgg
      601 aaataaaacg aatagaagat gggtttaaca aaaggaccag tcataggaag aaattgtcgt
      661 gaaatcgaat gataatctga acggtaggca aagactgtta aaaagatggg aaaggtatgt
      721 gcgatcattt tactgcacga ttatttggaa aataccttgt tgttcgcttt ttctggaaaa
      781 aatacataaa aaaagataaa acgattgcga actgcgtgta ttcggtcttt cgatcttttt
      841 cacggtatgg tatgatagtt aagaattttg agaaatttta aaaaataata atcgttacac
      901 aaaaatgttt tttaaagttt tcttatgatt aaaaagtgaa tttgctgatt gaacagttat
      961 atgagacaat attttagaaa atgtgaaaca gaggtgtatt atgaagcgat ggcaaaaagt
     1021 tctattaacc ttgttagggg tattcgtttt gctgattgcc ggattatcag catacggaat
     1081 cagatttatg ggagaagcga gccaaacagt aaacaaaatt tctaaaaact ctaatcgaat
     1141 atcctccaaa cgttctgaga gagtaagcat tgatgacaaa gaaccttttt cggttttgct
     1201 attaggttta gataccggtg gattaggaag aacggaacaa ggacgttctg atacgatgat
     1261 ggtagtaaca gttaatccga aacaaaaaaa atcaacgatc atcagtttgg accgagatat
     1321 ttatacgaat atcgtaggtt atggaacggt agataaatta aatcatgcct acgcatttgg
     1381 tggtgtcgaa atggccatgg attcaattga gcaactgctc gatattccta ttgatcatta
     1441 tgtaacgatc aatttggatg gcatggaaga tttgatcaat gcggtcggtg gcatcaaagt
     1501 gaataataaa attgacttta ctttggatgg tgtccatgta ccagtaggtg aacaaacatt
     1561 gaatggagaa aaaggtttag cctattctcg aatgcgacac gaagatccag aaggcgatat
     1621 cggtcgacaa cgtcgtcagc gggaagtagt gacgaaaatc gtcaataaag ttttaagttt
     1681 agatggcgta agcaattata gaaaaatttt aaaagcagtt gagaacaatg tgacgacgga
     1741 cttagattgg gatgacatgc ttgatgtagc gacgaactac accccggcgt ttgaaacaat
     1801 caaacaagac cagttgcaag gcgaaggaca aatgatcaat gatatctact atcaaatttt
     1861 aggaaaaaat gatcttcttt cgatccaaaa tgaactgaag aaacaattaa atataaaaac
     1921 aagcgataca ttacctaatt tgaaaaatga gaatgcttca atgatgttct ttgatgattc
     1981 ggaagatggc gattcgacaa acggtgatgg atcgaatagt gaagggtaca ccgattattc
     2041 ttcctattca gaaaacacat atgatcagta ccaagatccg aatacctatt atcagcaaac
     2101 agaagataca agtcagtact atacaccaag tcaagatact caaagttatg atccttacgc
     2161 tggacaggaa caaaatggtt atggttatga ccagaataat gagtatggaa caacaagtgg
     2221 gtactagaaa aaaatagttt ggttaataaa gagtgccacc taaattagac aaaaaa
//
LOCUS       DACDIP010000151         2268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDIP010000151 DACDIP010000000
VERSION     DACDIP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.155"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..425)
                     /locus_tag="IV257_002808"
     CDS             complement(<1..425)
                     /locus_tag="IV257_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8815862.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IV257_002809"
     CDS             complement(534..1565)
                     /locus_tag="IV257_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8815863.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          727 a          359 c          372 g          810 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt ttgcttattg
     2041 atggattgga taaattaaac catcacaacg agtggaaatt atatgaaatt tttcataaaa
     2101 attcatatag aaacaaatga cattttatta gaatatgact ttcattaaca taaatgtttt
     2161 caaatttcaa ttaaattgtt ttaatcaaat tccaatctaa atatttgtag aaaacaatcg
     2221 tctcctatag aataaattta taagtaacaa tataccaatc ataaatct
//
LOCUS       DACDIP010000152         2255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDIP010000152 DACDIP010000000
VERSION     DACDIP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.156"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IV257_002810"
     CDS             complement(<1..142)
                     /locus_tag="IV257_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8815864.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IV257_002811"
     CDS             complement(183..437)
                     /locus_tag="IV257_002811"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8815865.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IV257_002812"
     CDS             complement(548..802)
                     /locus_tag="IV257_002812"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8815866.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IV257_002813"
     CDS             complement(995..1270)
                     /locus_tag="IV257_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8815867.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IV257_002814"
     CDS             complement(1242..2195)
                     /locus_tag="IV257_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8815868.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          671 a          415 c          329 g          840 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattc
//
LOCUS       DACDIP010000153         2233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDIP010000153 DACDIP010000000
VERSION     DACDIP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.157"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(28..>2233)
                     /locus_tag="IV257_002815"
     CDS             complement(28..>2233)
                     /locus_tag="IV257_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8815869.1"
                     /translation="TNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGI
                     NPGGFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSK
                     IRKMQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTS
                     KTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTE
                     IADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGK
                     DERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIP
                     LTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYA
                     SFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNEL
                     SNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIIS
                     SPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTIND
                     SVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYT
                     LAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDIL
                     GVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT          744 a          464 c          290 g          735 t
ORIGIN      
        1 ggacactgtt taaatagtaa acgattctta attaagttta cttctatttt cttgaaattg
       61 aataattttt tcagaaaatc catttgcaac tttcccctca tctccaataa aatcaagata
      121 agtaaaatct gtcccaagtg aattattaac tattttatca atgaaaaatg aaattatccc
      181 tacacctaag atatcaactg tttcgtcatt ttctaataaa ctatttgtta ctgaagcgat
      241 tttatctgaa gaaccaaatc tgttcttaac aaattgatta aatctatcaa tagcaccctt
      301 atcacaatag tacgttttta aagccgataa agctgttgaa tcagaatttc tttttgctaa
      361 ggtataaaca tttaccgcgt caacatcttg gtataaatct tctagactaa atgttccacc
      421 tgcagccgta cctactaatg caaatacacc ttgctccatt tgtattccta atttcttaac
      481 ttcaccccag caagtcatta aatctcctaa ccaacctgca aagtcagaaa cactatcatt
      541 gattgtgtcg gctagaaaca tctgtgattg taacgtcaca attaagtgag gcaaacctat
      601 tattgaacca gtattaggat ctataaaata attatacagg ccctcgctat tagttaattt
      661 attcctatat tcatcataaa tttttctatc atgctctgtt acaggagaag aaatgatgtc
      721 ccatgacaaa ccagaataat ttacatatct ataaaatgtc aaatgagggt tccaaggatt
      781 atcaagacca gcttgtgata gatattgata cgcacgtacc atctgattta taaccttctg
      841 attgtatttg gcaatgtaat tatcattatt agtatcattt gataactcat taaatccgct
      901 atctctaccc gatgtagcga ttaaatcgat accaacagct ccgttgccac ttcctatggt
      961 aaattcaaca aattgatcaa aagaccattg tctaggcata ggaaaaccta aattcccact
     1021 ataaccagtc gacatatctg aaacaaatgc aaacgaagca tacccgttat tgattatttg
     1081 agaacaaaca tttcttgttc cgtaaacacc cgtttgatac ccgctatatg acattacagc
     1141 agataccccc ttaaaatatt ctccagcata tgcaagaatt tcttccccta acatgtctag
     1201 atcaacagca aaataaataa ctgttgttaa tggtatattt agattttttg ctgcatctat
     1261 agccttctta ccatctgata agccacgatc ataagtaaaa taagctaatg ctgcacctcc
     1321 atcttgatag ataggaaaca cagataaatt tttagaaaaa atattattca attcctctgt
     1381 agttagatac ttatttcgtt catcttttcc aattccaaca gtaccagtaa gatatctacc
     1441 aactattttt atatcattat caaccaatgt ttggatttga gatctagtta attgcgtaga
     1501 agtatctgcg ccatctgcag atcttctaat atctccagcg ctagacaata gtccttttat
     1561 tacaacaaca tctgctattt cagtatattc aggcaatacc ataaattttc ggaatgccag
     1621 tacttctaaa cccatatatt tagtgaaaat accatcgaat gtaccgtctt caaaataacc
     1681 attaacatat aatccatatt gaattattct tacatagttt cccgagttac cttcagacac
     1741 aggataagtt ttactagttg tcccattacc ataagaccct gttgcttcac tgggagaata
     1801 tcctagttca gcttgtaatg caaaaattaa tgcttgattt gaatttcttt gataaatacc
     1861 atcacaaggc attattcccg tgtattctaa ataatttaca tttaaccatt gctgcatttt
     1921 tctaatttta ctatctccgc cgggaacaag aacaaaagca ctcatatcaa ataacgcttt
     1981 ggcccatttc gcatccaaaa ctccagttac agcgcttcca ttaaaccctg catcagtctg
     2041 caactctttt acagcagtat ctaaatatgg agtatatacc ccatcgaagc cacctggatt
     2101 aatcccctta caccaaaagg caccttgaat aagaaataca atatttttag gtagattttt
     2161 gccccattta ggagtaacct gattatcgta cattttttca gtggtaggtc caaaattagc
     2221 tactagatta gta
//
LOCUS       DACDIP010000154         2155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDIP010000154 DACDIP010000000
VERSION     DACDIP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.158"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            135..1244
                     /locus_tag="IV257_002816"
     CDS             135..1244
                     /locus_tag="IV257_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8815870.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1327..2155
                     /locus_tag="IV257_002817"
                     /pseudo
     CDS             1327..2155
                     /locus_tag="IV257_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
BASE COUNT          715 a          326 c          457 g          657 t
ORIGIN      
        1 ctttaacagg gatagctgtt ttaactggga cattactcgc agcatgtggt gggggagata
       61 gtaattctaa agacgggcaa gtaactttaa gctatgcgag ttgagatact gaccaagcac
      121 caggactaag aaaaatgtta gatgaatttg aaaaacaaaa tcctgatatc aaagtagaaa
      181 tggagacaac gccttgggat caatattggg ttaaattaga agctgcaaca acgggaggta
      241 atatgccaga tgtagtaacc atgcactcta gcgaatccta taaatatatg tcacagaatg
      301 ctttgatggg acttgatgat gttatcaaag aaaatgatat tgacatggga aactttactg
      361 aaggaattgc tgatttctat acgcttgaag acgaactata cgcaatccct aaagatgctt
      421 ctgtagtggg gctatggtac aacaaagaat tatttgataa tgcaggtgtc gattatccag
      481 atgaaacatg gacatgggaa acactcagac aagctgcaat tgatttaaca gatgaatcaa
      541 aaggcatcta tggttttgca tcacatattg gaacagagac tggctactgg ccatttattt
      601 atcaaaatgg gggagaagta tttgccgatg acaatacaaa atcaggtttg aataccccag
      661 aagcacaaga agctttagct ttttatcgag atattgcact tgttgatcat gcttcaccaa
      721 caattacaga attacaagat cctgataagt taagtcgttt tcaatcaggt agattagcaa
      781 tgatgattga aggaaactgg tttgcaccgg gatttttgaa aaatgactat ctagttgaaa
      841 atgttgcttc tgccgtgtta cctaagggaa aagttaatgc aacaattaca aatggacttg
      901 gttgggctgc cagtgcgagt acaaaatacc cagaagaagt gaaaaaacta atggcatttt
      961 tagctagtga ggacgccaat aaaatccaag cggaaacggg ggcatcaatc ccagcactaa
     1021 aaggctttga agacgagtgg gctgcttctt cagatacaat tgatttgagt gcttttggaa
     1081 aaatgttgga gtatggagtc cctcgtccat acaataaaga tggattacga gctgaacaag
     1141 cagaagttca aattattaac cgtattctgt ctggtgaagt aactgttgaa gatggaaccg
     1201 ctcaaattga aaaagaagtc aatgcgatat tgagcggaaa ttaaattagt taattataca
     1261 atatacaggc gaaaggattc tatttcgtct gtatattaat ggatgaatac caaaaagggg
     1321 gatattatga aaaatcgaat ttttggaaaa tatcgtaaag atcaagtgat atggggctat
     1381 atttttattt ttccaacggc attaggatta attttattaa atatatggcc agcaattcaa
     1441 acagcaattt taagttttca aaaaacggta gggttcggtg atactgaatg ggtcggatta
     1501 agaaattacc aaaaaatgtt tgcagacggt gaagtttttc agtctttatt aaacacatta
     1561 atttatgcag tggtttcgat acctagtatt gttgtattat ccttgttggt ggcagtttta
     1621 atgaatagaa aagttaaagg attgtcgatt tatcggacaa tttattttct accagtcgtt
     1681 gctgcaccat ccgcagtcgc aatgatttgg cgctggatgt tcaataatga ttatggactg
     1741 attaataaca tgttggctaa aataggtctc gatggaccag catggttaac ggatccatcg
     1801 attgcgattt attctattat cattgtagga gtttggagtg ctattggtta taacatggtg
     1861 ttattactag ccggcttata agaaattcct aaagattatt atgaagcagc ggaaattgat
     1921 ggtgctagtc caatccagca gtttttcaac attacgttac cattagttac accaacatta
     1981 ttttttgtag ttgtaacaac agttatcaat gctttccaag tatttgatgt tatttttatg
     2041 atgattactc ctaatagtac cgccatgttt aaatcacaat cattagccta tttattctat
     2101 aaacattcat ttattttaaa tgacaaggga tatggttcgg caatcgttat gttcc
//
LOCUS       DACDIP010000155         2015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDIP010000155 DACDIP010000000
VERSION     DACDIP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.159"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1177)
                     /locus_tag="IV257_002818"
     CDS             complement(<1..1177)
                     /locus_tag="IV257_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8815871.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     "
     gene            complement(1311..>2015)
                     /locus_tag="IV257_002819"
     CDS             complement(1311..>2015)
                     /locus_tag="IV257_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8815872.1"
                     /translation="DADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKV
                     DVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPN
                     AEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSN
                     EKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILL
                     GTLGLLGYKNRKKENE"
BASE COUNT          590 a          378 c          251 g          796 t
ORIGIN      
        1 aaagttgtac acctactatt ctctttgtat ttttttcaaa gctaattctt aattttacac
       61 gactattttt tctcaaaaaa acaggtctaa tgaaatcttc tatttctata taatctatag
      121 actcacttaa taatcgtgct gtttttaaat tcaaacctgc tgcgtaaaaa ttgtaattaa
      181 atatagaaaa gccatttgta aattgggaac cagaaggtgt aaaatcttga ttgtcaatat
      241 gttgagttgc aatataagct gttcttagag cattagaaat agaaaaatca acactacttc
      301 tattaatgga cgtaaaaaaa gttgtagcac aatccccaat agcatatata tcttttatat
      361 ttgtttgctg gtattcatta gttagatatg ctccactaac atctctcaag agttcctttt
      421 tgccaattgg actattagat agaaatccta tagcttgtac gaccatatcc atagagtatt
      481 gggtaccact tgtagtgact attttttcaa tcttttttct tcctaaatat tttttaggac
      541 tttctgatag acaaagatgt attccatttt ttgatagtat ttcttcaact ttatctgaaa
      601 aactacggtc gtaataagtc gacaacactc tatctgcaac atcgaaaaga tatacttctt
      661 tccctctttt tcttatggct tcagcaattt caactccaat atatcctgct ccaataattc
      721 ctactttttt aatagtatta tcatctaaag ctttaaaaat ctctttagca tttggaaaat
      781 tctttatcga aaaaatatta gctaaattat tccccggtac atctaacata atttgctttg
      841 aacctgtagc tagtataagt ttgtcatatt cttgggaaaa tcttgtgcca tcttttttta
      901 cacaaataat ctttttattt tgaatatcaa ttttgtcaac tgctgtctcc aaaaacaact
      961 tttttatctt agaatttagt tctcttctat tttgataaaa aagatcttct aaattgctaa
     1021 cttctctttt tatgaacaac gatgtcccac attctaagta actaatttct gattttttat
     1081 ctatcagaat aacttcataa tttgaatcac ataagtttag aaatcgagct gcagctagcc
     1141 ccgaatggtt tgatccaata attactattt ttttcactta atcacattct tttcccttct
     1201 atgaaaagta tcttcatttt taactaacaa cctatctccc aaaataaaaa acaaaaagag
     1261 gaagaaaaag attcttcctc ttttcagaga agatttaaga aattgatttt ctattcattc
     1321 tctttttttc gatttttata ccctaataac cctaaagttc ctaacaggat taaaccacca
     1381 ctaatagtaa agatattact atcaatctct cccgtcttag gataagcttt tgaggactgc
     1441 ttttgaggac tgcttttgcc tctatttttg ttttgcttgc taccttgatt gtcagtctta
     1501 ttatctttac taacggcttt ttcactgtct ttttcgtttg aagaaacaac tacattagta
     1561 ggttcactag tattaccaga ttcatcagtt gcagttacta tcacttttgt tcctgcctct
     1621 aatttaggaa ctttaactgt aaaattacca tttttatcag ctgtcccatg gtattgactc
     1681 ccgtttggta atgtaactgt cacctctgca tttggttcag ttttaccagt aacaatttgg
     1741 tcaccctctg taataggatc aacttgaggt ttattcggtg cttttttatc ttctacagaa
     1801 atagtaacct ctacttcttc tttagatcca tctggatatg ttactactac aatccctttt
     1861 ttgtttccag ctgtactaac atctactttc tctttccaag tataagtagt tccttttggt
     1921 agatcttctt tgttcttaat tccctcagat gcgtctggct ctttatttaa gtctgtagtc
     1981 actttttgtc cttcaggatt gtatttatcg gcatc
//
LOCUS       DACDIP010000156         2010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDIP010000156 DACDIP010000000
VERSION     DACDIP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.160"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            347..1462
                     /locus_tag="IV257_002820"
     CDS             347..1462
                     /locus_tag="IV257_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8815873.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            1459..>2010
                     /locus_tag="IV257_002821"
     CDS             1459..>2010
                     /locus_tag="IV257_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HAP8815874.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIAS"
BASE COUNT          736 a          204 c          339 g          731 t
ORIGIN      
        1 ttgtgatttg gtatgaagca tttatcatat ataaaacggt atttgagaaa cgttttattc
       61 atttcttttt aactttctgg cgaagtttta tagaattaat aatttcggca atagtcgttt
      121 ttctattaaa tgaatattta gcaaatggag gcagtacgtt ccttcaattt ataactaaaa
      181 ccttaataac tatagtaaca acatcgatat tatatttgtt attatatttt agaagagaag
      241 aatttacatt tatcaaaaaa attcttttta ggtatatttt aaaaaaatag ctttctataa
      301 aggagtgttt tatttgaaaa tagctatact atctccagct ttctcaatgg gaggagctag
      361 tatcgtggcc tcaaaaattg caaaaggatt aatagatagt ggccatgaag ttttttttgt
      421 ttcttactta gaaagtgaaa aaccacaagt agaatctaag tattatgatt tatacgaaaa
      481 aaaaattttt ttatttaaaa atttttctcg atttcataag atattagagt atagattgtt
      541 tggaggcttt gttcctagta gatatataaa aaaagaaaaa gaacaactaa aaaaagttct
      601 aaagaaagaa aaacctgatg tcatcatatt caatactttt atacctagtg tactattctc
      661 cgaattccta aagtatgaat ttccaacagt gaaattaatt acttggatgc actctgatcc
      721 tgattacagc ttaaatcata ttgctaaatt ttataaaaaa gaatatagaa aaagttttga
      781 acgcgtggat tccattgttt gtctttcgca agatacaaaa gaaaaacttt cattatacaa
      841 taaccatatt aaagttattt ataacccatt gacattaagc ggctctagtt taataagtaa
      901 ccttgataaa aaaattattt catttactgc cagatacgat ataaatatta aagggtttga
      961 ttatttatgt gaagtagcta aaaacttacc tgttggatgg gctataagga ttgctggaga
     1021 tggtacagaa gaagaacgta aagagctgaa aagattaatt gagaaaaatg gtgtgttaga
     1081 taaaattatt tgcgcaggtg cattatttgg agatgaatta atcaatcatt atatagaaag
     1141 ttctattttc atatctactt ctagaagtga gggactacca ttagtgttga tagaagcaat
     1201 gacttatgga ttacctgtaa taagttttga tcatggtggt gctaaggaaa tattacaaga
     1261 tggaaattat ggaattttaa ttaagaattt tgacgtacaa gatatgaata atgagataga
     1321 aaaattgtct aataatcgtt tacttatgga aaaatatcaa aaattctctt tagaaagagc
     1381 taaggatttt aatgaaaaaa aaataattgg tgaatggcaa acattgttaa atgaattagg
     1441 aaatgaggat gttagaaatt gaggacttcg attaaaaatt ttaatttatg gaaagaatgg
     1501 tatctatata tattttttat agggatgatt attcgtgcaa taaattttta tagtttatta
     1561 ccgtccactt ttgatagtat tttttttaaa cttataggaa tttcaggagt tttgctatta
     1621 gggatagatt tcataattaa atttaataaa agaaaagtaa cttataacat tttattgata
     1681 gcatatatgg tgattttagt aataacgagt tttgttcata aagaatatgg tctaggagaa
     1741 aacttaaaga ctataatgtg ggcaatctta cagtattttt tgatttatca gtttgctttt
     1801 gagaatagcg ataataaacg attttttaaa aggataagtt ggggatttat ttatagttgg
     1861 tttgttttag tatcaatgtc aatggttctt ttctttggta aattcggcta tgagaaatat
     1921 tataatgcta gacataggat cagaattggt tttttagaat caagactgtt tgggacgttt
     1981 tctgatatta acaatgctgc tatagcatct
//
LOCUS       DACDIP010000157         1985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDIP010000157 DACDIP010000000
VERSION     DACDIP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.161"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..318
                     /locus_tag="IV257_002822"
     CDS             <1..318
                     /locus_tag="IV257_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8815875.1"
                     /translation="EARHALCRNIFYGRKGKLYQTYYDGMEEQLNALGLVTNAIIYWN
                     SLYLEKVFEQIKTEGFDCSEEVLRKQFPLISDHINFVGKYSFRYEKELSNGILRPLNI
                     ENY"
     gene            483..710
                     /locus_tag="IV257_002823"
                     /pseudo
     CDS             483..710
                     /locus_tag="IV257_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            836..1807
                     /locus_tag="IV257_002824"
     CDS             836..1807
                     /locus_tag="IV257_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8815876.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
BASE COUNT          673 a          302 c          395 g          615 t
ORIGIN      
        1 gaagctcgtc atgctctatg tcgaaatata ttttatggta gaaaaggcaa actttatcaa
       61 acttattacg acggtatgga ggaacagctc aacgcattag gattagtaac taatgctatc
      121 atatattgga attcactata tttagaaaaa gtctttgaac aaattaaaac ggaagggttc
      181 gactgttccg aagaagtgtt acgcaagcaa tttccattaa ttagtgatca cattaatttt
      241 gttggcaaat atagtttccg ctatgaaaaa gaattatcga atggaatact acgtccgtta
      301 aatatagaga attattgaga atgttcgtct tttggttcct accgtgcatt ttcgcacgga
      361 tgttccagat actatttttg aatgtataaa attgaaaaca caagaatact aataaatcaa
      421 cgattcttgt gtttttaatt tttcactacc gtgcattttc gtttctatgt tccatgtacc
      481 ccaatcttga aaaattaatt aaagacttag ataaagccat tgacgactta gtcgtggtgc
      541 taccggagta tcaatgttta accagtatcc caggtattgg tcctgtttag gctgctggaa
      601 tactcgctga aattggtcca attgaacgtt tttctgatga aataaaattg acaaagtatg
      661 ccggcctata ttggcgacag agtcaatccg gtaaatctga atacgaaaat ttttttacga
      721 aaagcttcac gtctaatatc tattttaaaa taaaaatatt cctgctaatt tggaatcgtt
      781 tccttctttt ggtataataa tacctgaaat tcttttgaga ggagttgttc gtgaagtgaa
      841 aactactatg aaggatgttg ctgcgttagc aggagttggt gtgggtacgg tatctagagt
      901 aataaatgga gtaaaggtga aagaggttac caaagaaaaa gtaatggctg caattagtga
      961 gcttaattat gagcctgatg aatatgccag aggtatgaaa acgaatagta gcaatacaat
     1021 agctcttatt ttacctacga tttggcatcc cttcttttcc gagtttgcct actatgtaga
     1081 ggaagctctc agccaaaaaa attataagct ttttttgtgt aatgcgaacg gcagtgcgga
     1141 aaaagaaaaa gaatatatcc aaatggtcaa gcagagtaaa gtagacggaa ttataggtat
     1201 tacctattca gacattgata aatatgtatc atcaaattta ccatttgtca gtatagaccg
     1261 ccatttttcg gaacaggttt cttatgtaac ggcagataat tatttaggtg ggcaaattgc
     1321 cgctcaagaa ctacttactc gtaactgtaa aaacttgtgc tatgttgggg gtgtttcacc
     1381 ttaccctaac gaaacaaaca atcgtaaagc tggtttttac aatatttgtg aggaaaataa
     1441 aatagctgtg aaggtattgg atatgcctga accaattagc aatttagagg aacaattgct
     1501 cgcttttttt tcggataatc ctgaaattga tagcattttt accatcaatg atttcatggc
     1561 tttggatgtg ataaaagccc aaaaaaaact agggaaggaa gcacctaagg attttcaaat
     1621 tattggtttt gatggagtca agctatcgtt gcaaaaagac tatttactat caacgattgt
     1681 acagccgata gaggaaatgg ccaaggtttc agtagaaaca ctcatggcga tgattttaaa
     1741 tgaagaaccg cctaagcgtg tggtagtacc ggttttcttt cacgctggag agacgactaa
     1801 agagtaaaaa accggtttat aacgattttt ttgagatagg ttgacagaaa gtgatttcaa
     1861 tgatatctta aaaccaatta attggaaacg ttaccaatta attgaaaaat aaaacggaaa
     1921 cgtttcaaaa aataggagga acatcgatga aaaagagaat gttagcaatt gcagcaattt
     1981 tagca
//
LOCUS       DACDIP010000158         1980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDIP010000158 DACDIP010000000
VERSION     DACDIP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.162"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            271..837
                     /gene="amaP"
                     /locus_tag="IV257_002825"
     CDS             271..837
                     /gene="amaP"
                     /locus_tag="IV257_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8815877.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            850..1038
                     /locus_tag="IV257_002826"
     CDS             850..1038
                     /locus_tag="IV257_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8815878.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1051..1608
                     /locus_tag="IV257_002827"
     CDS             1051..1608
                     /locus_tag="IV257_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8815879.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1640..1882
                     /locus_tag="IV257_002828"
     CDS             1640..1882
                     /locus_tag="IV257_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8815880.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          721 a          305 c          406 g          548 t
ORIGIN      
        1 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
       61 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      121 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      181 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      241 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      301 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      361 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      421 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      481 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      541 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      601 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      661 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      721 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      781 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      841 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
      901 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
      961 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1021 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1081 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1141 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1201 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1261 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1321 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1381 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1441 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1501 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1561 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1621 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1681 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1741 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1801 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1861 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     1921 atttgagaac cgtagcgaca ctcgctgcgg ttctttttta tatattttat atgaagtatt
//
LOCUS       DACDIP010000159         1963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDIP010000159 DACDIP010000000
VERSION     DACDIP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.163"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IV257_002829"
     CDS             complement(<1..281)
                     /locus_tag="IV257_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8815881.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGR
                     MANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYLHLY"
     gene            complement(429..987)
                     /locus_tag="IV257_002830"
                     /pseudo
     CDS             complement(429..987)
                     /locus_tag="IV257_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302360.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1156..>1963
                     /locus_tag="IV257_002831"
     CDS             1156..>1963
                     /locus_tag="IV257_002831"
                     /inference="COORDINATES: protein motif:HMM:NF019884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8815882.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDE
                     LSLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRY
                     SELLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIES
                     SMAKLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLT
                     KLYYLIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAI"
BASE COUNT          622 a          323 c          364 g          654 t
ORIGIN      
        1 ttgtacaggt gtagataagt tggtacttct tgatacggaa gcaactgaat ccaactatca
       61 tgataagcat gacggatgac agaatcaaac ccacaacatt cgcaacgatg cgcttctcca
      121 gttaataagc cggtaaatac attggccata cggccatgaa cgcgctcttt actaatggca
      181 tcttttgaaa aggtaatatg ggaatctttg atgttcaaaa tttgtttgat ggaatgactt
      241 gtcttttcat ccgtacattt gatattctta gttatggaca tttggttatc ctcctatgat
      301 tgtttttgcc gatttaatta taggacaacc agatgtcttt ttgttataaa aaaatatttg
      361 gcgtcactac tcagtttgag taacaaaacc aaatattata gaaccggctt tttcagtttt
      421 ttcccttcac aaacaacatc gatgattttc tcggcatggg tacgggtatc attcattaat
      481 tctgtatcgg ttggatattt gatgttttgt ggagcacaag tggcatccaa aatcaatgtt
      541 ccggaattga aatcgtcata agaatcgttg tccttatcgt cttttttctt gtcgaagacg
      601 agtatttttt cattgatttc tatcaacgtt gtttcgtcca agcgtttccg gaaatagacc
      661 attgtagagc atcgaaaggc ttggcatctt gatagcccgg caaaccgatg aagaactata
      721 aatacgggtt ttcttgaatt tgtaagaccg tttcttcgtc gctatagcta tattcctttt
      781 gaatcagtag agctccgaag gtcatgcgaa agggttttgc gacgtttcct tttttttacg
      841 aaaattttta gcgtagtctt tttcaagttc agcccatgga atgagctctg ctttttgaat
      901 ccaacggtta gacggattca ttttgagacc tatcggttga ttgaaatctt caaaagccaa
      961 ttgaacggtt gtttgtaatt tatacatgaa aagcctccaa aattgagtgg tactccacac
     1021 cattctgatg gtgcataaga taaaaaatta aagaagtgcc tgtcatttgt ctgatttaag
     1081 ccaaggtttg acaaattttg gatattccaa ttaatactgt aagcggttat atttaactca
     1141 gggaggtaga tgaatgtgaa tacaaaagat ttaaaactgt tagaaaccat tgtacgatca
     1201 catgtgacaa actctcaaca attgaaatca ttagtacatt tttcgactag gcagataact
     1261 tactctataa aaaaaattaa tgatgaatta tcccttcatg gattgagtct gataaagcga
     1321 agtaactcag gggagtggtt atatgaagcc aatgatcttc aaaaactttt gatatacatg
     1381 aatcaagcaa tggtttttaa agaaggtgcg attcctgaat atataaacga tgatttggct
     1441 tatagtgaga taagaagata ttcggaactg ttatttattc tagccaaccc caaaaaagtg
     1501 acaacccaag aaataatggc gtttattggt atcagtaaaa atacggctag aaacgacttt
     1561 tctaaaataa aaaaagagtt gccaccagac tacactttga tatttgatcc ccaaaatggc
     1621 tatatgattg aatcaagcat ggcaaagctt catacattgc tcttagaagt tttcaattat
     1681 ttatttgaaa ggcatgcaac agttaaccgt tttgagtcac tcttttctga agaaagggaa
     1741 tttgttggtt tttttattca cacctacgaa caaactttta aattgaaatt ttctgataat
     1801 tcactaacca aactttatta tttgataggt ttatcacttt ctagttttaa aagaaggacg
     1861 aaaggacaga ttgattttag ttatgattta caggaattag atgagtttca aatgattcga
     1921 agctgcattg atcaacatta tcaacatgct gagatagcta tag
//
LOCUS       DACDIP010000160         1927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDIP010000160 DACDIP010000000
VERSION     DACDIP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.164"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..1132
                     /gene="xerS"
                     /locus_tag="IV257_002832"
     CDS             47..1132
                     /gene="xerS"
                     /locus_tag="IV257_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8815883.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(1416..1787)
                     /locus_tag="IV257_002833"
     CDS             complement(1416..1787)
                     /locus_tag="IV257_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8815884.1"
                     /translation="MTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPE
                     TASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIV
                     GVHIIRIHGLRHSHASLLISQ"
BASE COUNT          689 a          329 c          300 g          609 t
ORIGIN      
        1 attatagaga tactcttctc ccctacttat tgaaaggatg aatgacatga aaaatacaga
       61 aatatatcaa aaaatagata caatcatact tcatatgccc gattatataa aagattttgt
      121 tcaagatcgt gaggataaag atcaatcacc tcgaaccact ttagaatatc taaaaaatta
      181 taagctcttt tatgaatggc ttctgagtga gtctatcgtt cctgatactt ctatcacctc
      241 gataagacag ttaactgcct atgacctaaa cttgtacaaa tctcatttga aacgtcgcac
      301 aaaagaaaat gtaaaaaaag agacaacaaa aactaagctc aaagataata ataacttagg
      361 tctatctact tctactatca atagaaatat cacggcatta aaggttctat ttaaatatct
      421 ttcgaaaagt tccaataatc ctctaggaaa accatactta gacaataatc ctatggatca
      481 agtagcaacc atcacagata aaactacgtt agctgcacgt gccaatgcca ttgagaaaaa
      541 attatttcta gataaggata cacaaaatta tctagattat attgccaatg aatataaaaa
      601 tactttgagt aaacgagctt tgatctatta tcaacgagat gtagaaagag acctggcgat
      661 caatgcactg attttaggat ctggattgag actctccgag gttataaata ttaatttaga
      721 tgacctatct ttagataaga ataatgtggt agtaactaga aaaggaaata aaagagatgc
      781 tgtcaatata gcagcattcg caatggaata tttagctaat tatctagcta ttcggaaaga
      841 acgatataaa gtaacagaaa acgaaaaagc tttatttcta gcaatttacc aaggagaagc
      901 caagcgtata tccggtattg caatcgaacg gatggtagca aaatattcaa aaggctttag
      961 aattcaagtc agccctcaca aactgcgtca tacattagct actagactct accaacagac
     1021 aaattcattg gtattaactg cccaacaatt gggacattca agtaccaata caacaacttt
     1081 atatacccat atagacaatg cggctacgat cgatgcatta aatgctttat gagtataatg
     1141 tataaatagg cggaaataag aaacaacacg ctagaaaagc ctgtattata gtttttctag
     1201 tgtgtttctt ttggtgtgat cctacttttg tttatcattt attttttatt atcgtaaata
     1261 ggtgtcggtt tgattttaga aaaattagtt tggatataat ctgtgggatc atttgttatc
     1321 tctctcaatg aatttagata caaatgacca taagttccta aagtcgtttg tacatcagca
     1381 tgttttaacc tttctttaat tcgaagtgca tctttttatt gtgatataag caaagaagca
     1441 tgactatgcc ttaatccgtg tatgcggatt atatgaacac caacgatcgt tgcatgtctt
     1501 ctcataatat gacggattgt atatttctga gtaggtgttc cattataaga aagtacaaac
     1561 gacatgcctc ctattgaaac ttgacgctcc ttccaagctt ttaaaaaggt aattgtattg
     1621 tcgtctagtt cgattgtacg aatactagaa gccgtttcgg gttctacaaa ctcataggtt
     1681 ttggctctct tataatagag tgttttattc acacgcaagt ttttattttc aaaatcaata
     1741 tctttccaag ttaatgcagt ggcttctccc aaacgcataa cggtcataaa aagtaaccac
     1801 atacaaataa aaccaaaatg ttggtacaaa tcatcaatat aaaaagttga aataactttt
     1861 gtaaattctt cttttgtcca aaaatcaact gtagttttcc ttttcttcac atttccaata
     1921 acttttg
//
LOCUS       DACDIP010000161         1800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDIP010000161 DACDIP010000000
VERSION     DACDIP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.165"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..95)
                     /locus_tag="IV257_002834"
     CDS             complement(<1..95)
                     /locus_tag="IV257_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8815885.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVA"
     gene            complement(107..595)
                     /locus_tag="IV257_002835"
     CDS             complement(107..595)
                     /locus_tag="IV257_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8815886.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(672..>1800)
                     /locus_tag="IV257_002836"
     CDS             complement(672..>1800)
                     /locus_tag="IV257_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8815887.1"
                     /translation="LRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCGN
                     YVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGEL
                     LLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMSG
                     LGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRSF
                     SIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNPT
                     IVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKPD
                     EDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT          561 a          389 c          263 g          587 t
ORIGIN      
        1 gctactacta acccggttaa atttagactc tttgtccaag tcatcgctac ttgtccatga
       61 attaatcgtt catcaattcg cactgcttta atcatttttc ttcctcctaa aaaaaatctt
      121 cctcttcatt tactacatgt acctcatcaa gaatggtcat ctttaattcc tgttggcatt
      181 tatttaccag ttcctgaata tcctcctttg ttaattcctt ggttgccaat actatttcga
      241 gcagaattgg cagattaaag ccagacaaaa tagttacagc aatctctttc tccaaaaagg
      301 ttttggtaat cttctgattg acacttccgc caaataaatc ggtaaaaaca aaaatctgat
      361 cctgctcgtt cacctggttt acaaatacat tcaactcctg ttcaaaatca cactcatcca
      421 cataagcatt aatcaccgta atctcttgaa ccttccccgt cagtaattga atactgctaa
      481 gaaagcctga agctaagtga ccgtgagtcg ctattagaat tttcttcata tggttaattc
      541 ctttctttct gttcactttt atataagcaa ttcctgtgcc aacttttaaa aacattggtt
      601 taatcacgtt aattcacatt aaaaaaagtg tctaacctaa aaaaaacaga ttagacactt
      661 ttttagacac tttatttttg aatatacggt cctaataatt catagagcag cgaaagctca
      721 taagcattga ctttaaaatt atatttcatc tccattgatt gaaaaatact tctagaaaca
      781 taataaaatt catcttcatc tggtttcaaa tcataattcg gttcttcaga tttttctgtc
      841 cgagccaaga ataaacgttc catcatcaat gccgtatgca tatagagatt tagttttaca
      901 cggccattta aagtgaaatg atagtaattt tcatatttag aaatgactgt ttccacttca
      961 ctcatcacga tcgttgggtt cagaaagttc aaacggtccg caattccttc caacgaaaag
     1021 aattttacta gctcctgaat caagcggtta aattctgccg gcttcatttc aggactcaaa
     1081 atatgctcca aattggctgc tccatcaccg tctaagatgt cataaacatt aatattagga
     1141 attgaaaagg aacgtggaag atccgaagtc gtgatgacca attttgtctt gtcaaaataa
     1201 gtttcatcat tttggtcgat taattcacgt agttctctgt agtccttcgt gaaaacttcg
     1261 atggattcat ccgataaatg atgacgaaaa acatctttca atttgtccga aatccccaaa
     1321 cccgacatac aggaaatgat aatgttgcta ctttgagaaa agccttcaaa gtattgaaca
     1381 ttgattgaat acttgtctgc tgcatcctcc gcaatctgtt taaatggcgc attcatcgtc
     1441 atcttcaagc caatatcaag tgcaatggaa gtcgtcaaat tattcagaag aagtaactcc
     1501 ccttttaaat cgttcttgat ctgtgtgtaa atttgattta aagagcccat atctacgatc
     1561 agcactagac tatctgtatt cacttgccgt ttcacgaaat tccttgtcga ttcgacaatc
     1621 tctgacacat tactctctac tggcatatcc aacgcttcaa aaacataatt gccgcataat
     1681 tgattcacta ctgcttgaat gctgctagcc gtactatctc cgtgagcaag caacaggccc
     1741 ttcagctcga tggattcatc tacataatca gataaagtca cgacaataag cagtcttaga
//
LOCUS       DACDIP010000162         1734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDIP010000162 DACDIP010000000
VERSION     DACDIP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.166"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..519)
                     /locus_tag="IV257_002837"
     CDS             complement(<1..519)
                     /locus_tag="IV257_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8815888.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGN"
     gene            774..1340
                     /locus_tag="IV257_002838"
     CDS             774..1340
                     /locus_tag="IV257_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8815889.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            1485..1572
                     /locus_tag="IV257_002839"
     tRNA            1485..1572
                     /locus_tag="IV257_002839"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1517..1519,aa:Ser,seq:cga)
BASE COUNT          646 a          275 c          324 g          489 t
ORIGIN      
        1 attccccaat aaaataccta tcagtaaagg aaaacgcata ccaaaagtga tataactaac
       61 gaccggtgat aatacgctac caaacagtcc gatcatgatg tactgagaaa aaggtttatt
      121 gacaaataca gcgtatagat acacacctaa aatgattgag atagaattat aaaggttttt
      181 gccaaaaaaa gagaaaccgg taactgttag aatcgaagca aataagggac cgtttaaaat
      241 caccttgtgc cgataagcta aaagcacaga aagtaaggtc aaaatcccta catttatgaa
      301 tgcgcttccg aaaacaccta aagcaaaata atctgtcaaa agattgcttg gcgaggtaag
      361 aatagttagc aatccactcc aattatcagt aggagtatta gaaagcaagc caataaaaag
      421 aaaaaaaaca cctgtaaaac caagataaaa atactttctt ttctgtgaaa cgattcgttt
      481 ttgaggatga atataaataa cgtcagtttc gttttccatt acacaccttc tttttcattt
      541 tctttaacta taacttgaaa tgaaagattt ggacaactat catttaggtc aaaactaaaa
      601 atgtaaggga acccattgaa tttaaccaaa tttttttaat tttgcaaaaa aaagttaaag
      661 aagactgaaa aaaatttaaa atataacgtt ttgagtaaaa tgtttgcaaa aaatgctaaa
      721 aatagggtac aataaaacaa catatactgc agtaaagaag tgggggagtt ttaatgagaa
      781 gaaaagtcta taccaaggat caaattctaa aagctgctta tgatgttgtt gcaaaggaag
      841 ggtttaaagg atttactgcg agaaatattg ctaaaaagat ggggatttcg acacagccga
      901 tttatctgga attcaaaaac atggaagatt taaaaaatac gttgctggat tctatctttg
      961 aacaactaaa agaagatgtt ttccctattg aacgtactgg tgataaaatc attgatctcg
     1021 gtttgaacta tatctacttt gcaaaaaagc atcatagtct ttacatggca ttgtacattg
     1081 atgaacatgg ggacggaaag aaaatgcatg atttttcata caattatttc cgtgaaatag
     1141 ttaaagaaga tgaaagatac aatcaattat cggatgaata tgtcgatgct ctgcatactg
     1201 gtacatgggt agcagtgact ggcttagcgt cattgatgtc atcaaacatc atgcatccaa
     1261 cagaagaaca aatcattgca ctgatcaaac ggacaattga ctcgatttta gcattagata
     1321 atcctgaaat aatcagatag tgttctatca gtatgaattt gatcggtaag tccgttggct
     1381 gcaaactgtc aatggacttt tttgctaaaa aaagcgattt ctgttattta tctattagaa
     1441 ataggcttga catttcgtga aaaaagttta aaaatagtac ttacggaagg ttggcagagt
     1501 ggtaatgcac cggactcgaa atccggcgaa ccggctaata ccggcgcgca ggttcaaatc
     1561 ctgtaccttc cttatatcaa cgattagagg ttgtgaagaa gctgtcctgc atcaagtaat
     1621 aagaacggcc aaacaaaaat agcttttcct attttttcgt ttgattgggc ttattaccgc
     1681 agatgcaagc ttttgaacac cgtttgttcg gaataagggg ctgtgacaag actt
//
LOCUS       DACDIP010000163         1713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDIP010000163 DACDIP010000000
VERSION     DACDIP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.167"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..136
                     /locus_tag="IV257_002840"
     CDS             <1..136
                     /locus_tag="IV257_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8815890.1"
                     /translation="LFKYRKYEEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK
                     "
     gene            212..577
                     /locus_tag="IV257_002841"
     CDS             212..577
                     /locus_tag="IV257_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815891.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(638..1201)
                     /locus_tag="IV257_002842"
     CDS             complement(638..1201)
                     /locus_tag="IV257_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815892.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          532 a          328 c          258 g          595 t
ORIGIN      
        1 tttattcaaa tatcgtaagt acgaagaagg agaagaaatt ccaagcatca ttgaattaat
       61 ctatatcgct gacttttacg atatttccgt tgattaccta attggtcgct cagaaacagc
      121 aagcaaactg aagtgatttt ttccagcctt ggtgctggtt ttttcattta acgtctaagg
      181 ttaacaatct atatataaag gagacatttc tatgcgatta tggcatgaaa aattgatttc
      241 aaagcttcct cgtcaacagc ttttaggaca acatcgggaa tgctgtgcat taagaggaaa
      301 tggctgggga aagaaacatg ctactgtgga ttatgttttt cactattctc cttatagatt
      361 attccaatat catcagcttg tcatggatga aatggaaaaa cgtggttaca gcccagcacc
      421 agagtggaaa aatcctatgt atcgtggcaa atcatgcgaa gcattttttg atttacctac
      481 tgtagagcgc aacgtgccga tttatccgga acacgatgaa gtttatctag cagaatgttt
      541 agcaaatttg gagcaaaaag gaatttatct tacctaatat aactagaaaa gccttctatc
      601 tcctcttact tcattaaaaa gctttaacca tcttagatca agctaaaatg tcgtaagttt
      661 tagggttatc ttcatcctca cgtgcaaaac taaaagcgta ctctagtttt ggttcatcgc
      721 caacttccct agacataaat gaacatctcc tgacgtggtt taaaagtacc gcatcttcga
      781 cgtctgtata cgatgcacag cacagattgc ttcttttgat ctttcttttg atttttctgt
      841 tgcttcttct acaccttctt cgattactac atcgatgctt acaattgctt ctgcctaata
      901 ttcccatgca tcgtcgattt aagcctctgt agtcactgtg atcgtttcca cattttaact
      961 cctctgtcgt ggcttcagtt gattttgtga tcagttcagt tgctaagcca gagtcagaac
     1021 aacctgccat tgctagcgtt aatattgata gtaaaaatag tttttttatc ataaggttcc
     1081 ccccctgaaa atagactaaa ataataacgc tttctttttt tgttttcaga cgaaaattaa
     1141 taatttctaa atattcttta aaaaagctta taactattat gcctttttta atatataaca
     1201 cagttatttt taaaaataag aatgctaaat cactctacaa taacggcgaa agcacaaaaa
     1261 ataaaaaaat tattacgtta ttaaaaaaac agatgaaaga tcaggtttta ttagatctca
     1321 ttctttcatc acttgaaggg atttaatgaa ctactttttc ctctttgtcg tttttatata
     1381 ttcgtattcc ttcaaacagt aggatagcct ctttcaatgc ttgatatttg aactgtccgt
     1441 tacttaaaaa tttagatgtg ccgccctctt cctagaacat attctttttt actttttcat
     1501 taattgacag accaatcgaa cgaaactgta aatttcgata ggataatgat tggtcaaact
     1561 cattcatttt caagacatct cgattcatat tcaagatagg aatcttttta tcaacatcac
     1621 tttattcgta ttttcctcta ccataaaagc ttcacgctcc acaaaattgt tttatacact
     1681 actattcagc ccattcaacg accaatgaat ttt
//
LOCUS       DACDIP010000164         1688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDIP010000164 DACDIP010000000
VERSION     DACDIP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.168"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            40..699
                     /locus_tag="IV257_002843"
     CDS             40..699
                     /locus_tag="IV257_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8815893.1"
                     /translation="MREGKQALSSKELTDWLDAVRDDFNDQLLSFHDYTLFMLTLYLG
                     DRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTIIQVPEIVMTLLQEWK
                     SVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRINSIKKRHPHLVHLTP
                     HKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLVDKAVFEAFQRGLQKS
                     N"
     gene            complement(857..1474)
                     /locus_tag="IV257_002844"
     CDS             complement(857..1474)
                     /locus_tag="IV257_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8815894.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
BASE COUNT          546 a          308 c          250 g          584 t
ORIGIN      
        1 attactgcac ctaagaaagt tgctctggaa gaaaaaagaa tacgagaagg aaaacaagct
       61 ttatcttcga aagaactcac tgattggtta gacgctgtaa gagatgattt caatgatcaa
      121 ttactttcct ttcacgatta cactcttttt atgcttactt tatatcttgg ggatagaaag
      181 agtgagacct atgctttgca atggaaatac attgattttg aaaaccaaac agtccgtctt
      241 aaatatgcct tggataaaca cacaaaaaag aaatttacaa aaggtcgaaa agatactatt
      301 atccaagttc cggaaattgt aatgacacta ttgcaagaat ggaaatcagt gcaagctgaa
      361 caactattga agttgaaaat taaacagaca cctgaccaat atctttttag ttactccaaa
      421 ccttctggtg aagtaaattg tccagtccat actgattact taaattatcg aattaattct
      481 ataaaaaaaa gacatcccca tttggttcat ttaactcctc ataaacttcg acatacttac
      541 gcaactttag ctagacaagg tggggctgat atgaatcaaa tttctaacgc tttgacacat
      601 tcaggtattt caactacaaa aatttatgtg aatacacctg atttagttga taaagctgtg
      661 ttcgaagcgt ttcagcgagg attacaaaaa tctaattaat gtgttctaga atgtgttcta
      721 aatataaaaa aaagacactt tccaaataat tggaaagcgt cttgaacgtt gatatataaa
      781 ggatattaac gttttgagaa ttgagatgct ttacgagctt tcttaagacc tggtttcttg
      841 caaaattata ttagcctcaa ctgaaagatt tatttttttg ctccctaact tcattagtaa
      901 taccatgttt cattcgatat cttctaaacg ttctacgatt aatccctgtt agctcttcaa
      961 cttctttatc tgtagatcca gtcaaaaaca aattaaatgc taattgaagt cgcggatcat
     1021 tctcacgata ttttagcgta cctcccttgt atttcccttt tgctttagcg agtgcaattc
     1081 cttgttgttg cgtttcgcga atataccgac gttcttcttc ggcctggtac ttgtatagtt
     1141 cgataattaa gttattaagt aatcgacgta aattatcatc agtgatacct gttaacgctg
     1201 gcaaattcaa aacttccagt gtagctttct tcagctgaat taaattcatc gtttctgtta
     1261 gctctttatt atttcgtcca agtcgtttta attctgtcac aacgacaata tcaccttcac
     1321 gtatgaattt catcatttct tgaaatcctt ctcgattggt atctttccca cttgctttat
     1381 cactaaaaat tttatcacac tctgataata attctaattg tcggtctaaa ttttgatcta
     1441 agctgcttac tcgagcatac ccaattttcg ccataataaa actcctttaa aattctgaaa
     1501 tccggatcat ttttaaattc tgttgcccaa ctctgttgtc atttttctag ataaatggca
     1561 taaattttca gcgctaagtg acaaaagcaa ctaatttttc atgatagatt tttaaatatc
     1621 tcaatctatc tccaaaatta gttgcttttg tagctatagt tgactatgaa tatgacaaat
     1681 aattagaa
//
LOCUS       DACDIP010000165         1674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDIP010000165 DACDIP010000000
VERSION     DACDIP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.169"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            385..911
                     /locus_tag="IV257_002845"
                     /pseudo
     CDS             385..911
                     /locus_tag="IV257_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1066..1641
                     /locus_tag="IV257_002846"
     CDS             1066..1641
                     /locus_tag="IV257_002846"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8815895.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
BASE COUNT          584 a          267 c          329 g          494 t
ORIGIN      
        1 attattttgt ctacctcttt tctacaagac aaagtatagc aagcaaaatt cttctccact
       61 tccagaattt tttattttca tttttagaaa agtgaaaaaa ctgacaacaa aagcttgttt
      121 ctttcactct attaatatct ttagtgaaaa agctcgtttt atatcgtatt cttccagcga
      181 atgtacttct cataagtcca aaaaaagctg cgaaaaagaa agtacctcgg tacctacttt
      241 ttcgtagctg cacataactt gagcaagtat taatcgaaga tacttttgtt gagagtgtaa
      301 aatattttgt gtaaatagaa aaaaggaagt cccttctgta gaatagagtt accacaacac
      361 attcacagaa aagaggactt ccatatgaac gattttacta cagaaatcct aaagactcta
      421 gcgaacaaag gcgatttgaa tggattattc cgtgtccatt tggaaaaagc tgtcaatacg
      481 cttctcaaaa cggagttaac ggctttcctc gattacgaaa agtacgatcg cattggtttt
      541 aacacgggta attctcgtaa cggctcctat gaccgtacgg tcaagaccga gtatggggaa
      601 cttcatctcc agattccgcg cgaccgcaat ggcgagttca agcaacagac tgttcctgct
      661 tatagacgga cgaatgacac attagaggag accgtcattc acctcttccg aaaaggtatt
      721 accatgtcgg aaatcgcaga cttgattgag aaaatgtatg ggcatcacta cacgccccaa
      781 accatgtcca acatgactaa agtgctgact gaagaagtaa atgcatttaa agttagaact
      841 ctaaatgata agtatgtcgc tattttcatg attccttcag tttttcagat gatataccac
      901 tcaaaaaaca atatgagaga tgttcaggaa taccatctct aatggcttat aaattggttt
      961 tagggatagt agatgttata gacatatcaa ggaataaacg agcagctgca taaaagcaga
     1021 tggaattaag gaataattga attgtaaaag aagggaataa atttaatggc acaattattt
     1081 tacagatatg gaacaatgaa tagtggaaag agtattgagc tactgaaagt ggctcataat
     1141 tatgaagaac aagggaagag agttatcata atgacaagcg caatagatac tcgatcagga
     1201 tttggaaaag ttgagagtcg tataggtgtt tcgaaaccag ctattgcaat tacttctgaa
     1261 atggatgtgt acggagaatt agtggagatt attgatcagt acggaaaagt tgattgtatt
     1321 ttggtcgatg agtctcaatt tttaagtaga gagaatatta ttgatctatc acttatcgta
     1381 atgaatgagg aagtaccagt aatttgctat ggattgaaga atgattttca aaataatctc
     1441 ttcaagggat ccgaagcatt attgacattt gctgataaaa ttgaggaaat taaaactatt
     1501 tgccattttt gcaataaaaa ggctgtaatg aatgctcgta ttgttgatgg tattccagtt
     1561 tattcaggtg aacagattgt agtaggaggt aatgaagatt acctttcggt atgtcgcaac
     1621 cactactaca acgaagattg aaataaaaaa cgtgtaatca aataattgat taca
//
LOCUS       DACDIP010000166         1658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDIP010000166 DACDIP010000000
VERSION     DACDIP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.170"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            60..917
                     /locus_tag="IV257_002847"
     CDS             60..917
                     /locus_tag="IV257_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8815896.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            918..1466
                     /locus_tag="IV257_002848"
     CDS             918..1466
                     /locus_tag="IV257_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815897.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          631 a          225 c          350 g          452 t
ORIGIN      
        1 aaaataaata tgttataata gtatcaaatg aatattgcta aaagaaaaga ggtaggaacg
       61 tggtagcgaa gaaatatgta atcggtaatt ttaaaggtgg cgtaggtaaa agtacgtgcg
      121 cgcaaatgtt agggtttgaa tcagcagtta atgaaggaaa aagaaccttg ataattgact
      181 tggatatgca aggaaatact tctgatgtga tgaacttaac gcatatgaac ttcactaacg
      241 aagaaggagg cggagaaggt gaaccgattg tttacgaaaa tactgtgact gacgtgttga
      301 taaacaatct tccacctaaa gaggctgtct ataaagtaat caataacctc tatatacttc
      361 cagcagacat gtcctttgaa atgtatgatg attggatcaa agaccaatat aatgattcat
      421 tatcccaatt caaatatatg aaagaaaagc tagaaccatt atttgatgag tttgacgtca
      481 tctatttaga tgtcccacct tctatttctg tatatagtaa atccgcgatg tacattgcgg
      541 attgggcaat cgtcgtctta cagacccaag taaaatcgat gagaaatgcg atgcaatatc
      601 ttgagtatat ggactttttt gtgaaagaat ttgatacaaa tttatatgtt gctggtgtta
      661 ttcctttcat gttggaaagc ggtgacgcag tagatcagga aatgtataaa caagcccaag
      721 agatttatgg ggaacatttg attaaaaatg ttgtactcaa aaatgcgcgc ttaaaacgat
      781 acgacggatc aggtattacg atggagaaga caaagaaagg tctcttaaaa caatgggatc
      841 gcaaaagcca cgaattattt atcagtattt tgaaagaatt ggaagaacac gagaattggt
      901 atgcggaggt ggagtaattg gctgacatca aaggacttga tatttttggg aacaacaaag
      961 gactcgccaa aataaaaaaa gaagcagaag caaagaaaaa gcagcaagca attacaccat
     1021 ctgaaaataa gcaattagaa gtgcaccaag ttcaagaaat aaaaacttcc tccaatgttg
     1081 aaagacagag tagaggaaga acaaaaaaag ttggcgcacc aatcagaaag tttgataagg
     1141 tttattccac caagcatcct ttaaaactgt cgactttgtt aaatgcgact tccagagttt
     1201 tagtagaaaa gtatgagact aatatgacaa gagatgaatt gcttagaaaa gctttagatg
     1261 agtatattaa acagaatctt acgcaagaag ataagcaaga tttgtttaat gacgtggtga
     1321 aagaactaga cttgttcaga gaaaaatatc caattgttcc agaattagat gatcgtggag
     1381 aaataataag aactaccagt cagataaaac aagataccga agactcactg cgcaaagggt
     1441 ggggaatgaa taataaagtt agataataat caaagaagaa ctaagtaact acctttaaac
     1501 ggtagttact tagttcttct ttgatttgca tgaaatagag gggatttatt tttctggtta
     1561 cgtgattaaa aaatccaaaa aatgagcata ggaacggtta tgattttagg gtgtgtttta
     1621 atgagatatt gtgatttcat taacaagtgt atgataat
//
LOCUS       DACDIP010000167         1637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDIP010000167 DACDIP010000000
VERSION     DACDIP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.171"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(184..1503)
                     /locus_tag="IV257_002849"
     CDS             complement(184..1503)
                     /locus_tag="IV257_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP8815898.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          486 a          301 c          325 g          525 t
ORIGIN      
        1 aattctttgg cacattttat tgaaatatgt atcactaata tccctatcaa ctgattttga
       61 ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg aggaataaat tttgggtgta
      121 aggcattttg agcatacttc tccctggata acttcccagt aaaaaaatcg ttagattgtt
      181 ggattagatc cattgactgg ctcgcctcag gcgagcaaag accctgtaga attcattctg
      241 atacacatga taactagata atttgagata gactcgtcta cccgtttgga ctaatttccc
      301 agcaactttc agaaacttca atcgaataga atgaatggtc attccctttt ggacttcttc
      361 aaagccaatc gttcttaaga agttgactaa gttgtaagct atcaagctga gcatcattcg
      421 gacatgattc tccaaaaagc gaggactgtc tgttttgtca aagtaaaagc cagctttcgc
      481 ttctttaatg aagttctcca ttgtgccacg tttggcatag atagaaaaga tggtatcagg
      541 agaaacattt tctgatagat tcgtcacgat aaattcatgt cgaaagagta gttcgcccgc
      601 ttcacgtgtt gagcgtatac acacgcgacg actttgtgac caggattgtg cttgataagt
      661 ggtcgagaag tactgaactt ctcgttcttc ccacttttga ttatcgccat aaagtactga
      721 tttctcagct atttgaccta gtctacgatt attcttcagt cgaataacat aatgactctt
      781 ttttgattca cacgaatcat acacaccagg tgtagcgaac ccgctgtctc cacgaaccaa
      841 gatgtcagtg tttggtagag agtgattata gtgctctaat aaaggtgtga gaaactcctt
      901 tacgcctttt gatgtatatt gatttcctga acgtagttca gcttttaaga agtcaccagt
      961 caatccgtca aaagctacca atggatggta accataggtt tggtagtgtg tattataatt
     1021 cgtttgttct tggtgaccaa atgtatctga atgggtcgaa tctaaatcaa tgattaattc
     1081 cgtatcatta cggatgaggc gtgctttatc gataagttct tggttcaaag cttgaagttc
     1141 atggatattc tcctcagata gtcgatctaa aaatcgggaa agtgaagatt gagaagcgag
     1201 ttctttccta tctaaaacag ctttaaacac aggatcttgt ctcaggagat tagctgcaga
     1261 atcagctgag taaccagcaa ttaattgcat aatgaactgc tcaagtatgg ataagttgtc
     1321 atgagtaaaa tacgctcgtt cgtcttcaat gtgaatgtgt tgcttagcca gttcagaaaa
     1381 accaaagatg ttcatcagct ctttcactag gacgagaccc gaatcactcg ataattgacc
     1441 acctgtatga gaaatagtga tatttgaatt gaattttact tggtttttgt gtaagctagt
     1501 catgaaaaga actcctttct tttggttggt ttagtcactt taaccatagc agaaaggggt
     1561 tcttttttca tcacttaacg ggtgaagatg aaaaagtaat tgtaagctgc cgtgaggcag
     1621 tttcacatgg ttttaat
//
LOCUS       DACDIP010000168         1629 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDIP010000168 DACDIP010000000
VERSION     DACDIP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.172"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..239
                     /locus_tag="IV257_002850"
     CDS             <1..239
                     /locus_tag="IV257_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAP8815899.1"
                     /translation="QILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEEQVAKLRE
                     LFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            259..1479
                     /locus_tag="IV257_002851"
     CDS             259..1479
                     /locus_tag="IV257_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8815900.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1537..>1629)
                     /locus_tag="IV257_002852"
     CDS             complement(1537..>1629)
                     /locus_tag="IV257_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8815901.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          553 a          299 c          328 g          449 t
ORIGIN      
        1 gccaaatatt ggattcacca gagttaaagg cacgaattat gccaagctca gaatttttat
       61 tgggtcattc actccaagcc tttgatgagc aaggacgact cattgatgaa gaacaagtcg
      121 caaaattacg tgaactattc aaagatttct tactctttgt cacaatcact agtcaattgc
      181 aacatgcgca ccaagcaaat aaacgtgaag ccgaaagctt ttcatgggaa acgatttaat
      241 cgaggggagc aaacaaagat gaaattaata ggtatcgtcg gatcagcagc tgaactttca
      301 tataaccgta aattattaac atttatccaa aaacaatatc atgaactatt taatttagaa
      361 atcattgaaa tcaaagattt acctctgttc aatcaagatg atgatcaaac aaatagtcct
      421 gctattcaac agctaaatcg caaaatctta atggctgatg gggtaattat tgctacgcca
      481 gaacacaatc atacggttcc agcaggattg aaaagtgcac ttgaatggct atcatttaaa
      541 atccatccac ttgaaaataa acctgtaatg gttgtgggtg cttcttacta cgatcaagga
      601 acttcacgcg cgcaattaca cttgcgccaa attttggacg caccaggcgt gaatgcatta
      661 gtgatgcccg gaaatgaatt tttattggga aaagcaaaag aagcatttga ccaagaagat
      721 cgcttgattg atccacgaac tgttgacttt ttaggcagta cattagcaag ttttgtaaaa
      781 ttcattaaag tcgtgtctac cttagcaggg cctaaaaagc ttccaccaga agatttgcat
      841 gcgacaggaa caattgctac gacaataccc gatgttgata tgtcagatga tcattggttg
      901 gaaaaagctt ctgaagcagt cggtgccgtt tctggaaata cttacgtcaa attaaatcgt
      961 gggttattga cagtggatca aatcaattac tttttagatt cgatgccgat ggaattaact
     1021 tttgcagata ataataacca atttttatat tataatttgc gccaagaagc gaaagagatg
     1081 ttagctgctc gtgtgcccgg tcaggtaggg aatccattag ccaactgtca cccagaaaaa
     1141 tcgtataaaa atgtagagtg ggtaattcaa caactgcgaa gcggagcaac agatgcggta
     1201 cgtgttcatg taccaaaaca agtgccagaa aaatatattg tgcataatta ccaagcaatg
     1261 catgatgaaa atggagagta tgctggaatc aatgaataca ttcttgattt caaaccgatc
     1321 attgagtggt atttacaaca aacgggtcaa caacttgttg gaaatgaaga tgcggtttca
     1381 agtgcatctg ttcatggtgg aaaaacagat gcagtaacca gcgccagcac acacgatgaa
     1441 actgcagtag atgcagtttc gagtgcttct gtaaaataaa aaggttctgt tgcaaagttt
     1501 taaataaaga ataaagtccc ttacggtatc tatgatttaa gctgggattc ccaataatac
     1561 cttgatttca gtacagaccg aaaacccgaa gagagtgcct tcttttcggg ttttcttata
     1621 taatcctcg
//
LOCUS       DACDIP010000169         1591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDIP010000169 DACDIP010000000
VERSION     DACDIP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.173"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..502)
                     /locus_tag="IV257_002853"
     CDS             complement(<1..502)
                     /locus_tag="IV257_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8815902.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSD"
     gene            514..987
                     /locus_tag="IV257_002854"
     CDS             514..987
                     /locus_tag="IV257_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8815903.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(1160..>1591)
                     /locus_tag="IV257_002855"
     CDS             complement(1160..>1591)
                     /locus_tag="IV257_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8815904.1"
                     /translation="LRMSDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILL
                     YTEGKEENDWLFPSRQQGRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKK
                     TRDIATLMFIFNHSSQAITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT          512 a          284 c          253 g          542 t
ORIGIN      
        1 catcggaata ttgtacgcgt agttgaatgg catcaattcc taaaatggca tgctctgtag
       61 ggatctggtc tgtgacatac gggaaataat catcaatatc ttcaaattcg cgtaagggaa
      121 ctaaagctaa tcgtgttgca tctggccctt caaaacgtaa gataggtcgt ccagaaaatg
      181 attcgattcc ataatggcaa acgccttgtg tttcaaaata tatttcccaa aaatgcaatg
      241 atgcttcgga aggaactttt agtatcgttc gttcaatcgt attggttcca aaaaactggt
      301 tgttcccttc ttgtacttca aaaaaagtca gttcagttcc cacacggtgg tgattatctg
      361 aaaaaaacag gtgatacatg gtatgatcgt cttgattaat tgttttcatt agtaatttta
      421 aaccgagtat attatgataa aagtcaaatg tcggtcgaat aaagcgattc acaatagaaa
      481 tgtgatgaat acgtgcgggc ataatatcta aaaatgagaa tcaaacctat aaaagatggt
      541 gaacaacttt tgttttcatc agaagaaaat aagccccttt atttagacta tgtaaatcac
      601 aatttgaaaa tcatcattaa agagaataac ttaaaacata tcacacttca tggttttcgt
      661 catacccatt gtagtctgct ctttgaatct ggtgcgtctg tcaaagaagt gtatgttcga
      721 cttggacata cagatattaa aaccactatg gatgatatat acacccatat cgcgaaaaag
      781 cagacagagg aaacggcaat tcgttttgca gatttcatga gttctacaga ggaaaagcaa
      841 ggaagtttgc agaaggtcaa aagtatggtc aaaaacgaaa aaagcagact atccctatta
      901 gatagtctgc taaacgttga gttatcagta tttttcttat tccgcagcgt ttgcgttttt
      961 gagaccgtat tgattctctt tttttaattt catccttttc caacctaaaa atgttattta
     1021 attacttagt ttatataaca cgaacctata ccaagtacca gtaaccccag cttttattcc
     1081 gcgaacttta tgaaataaaa tcgttctaga taaaaatagc ttttaatctt tgtctttact
     1141 gtttataaaa atttttaaat tatacaccta gtttaaatcc acgaagtgat gctcctattt
     1201 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
     1261 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
     1321 atgtatgtgt tcctatatca tctcgtccta atttttctgc tatatctgca tatatctgat
     1381 agacacgatc ccttgttata tgtcgacctt gttgccgact cggaaataac caatcatttt
     1441 cttctttacc ttcagtatat aataaaattt cttctctcaa attatctata aaaatcggtt
     1501 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttgcctcgt atatctccaa
     1561 cttttaatcc tataatatct gacatacgta a
//
LOCUS       DACDIP010000170         1587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDIP010000170 DACDIP010000000
VERSION     DACDIP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.174"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..824)
                     /locus_tag="IV257_002856"
     CDS             complement(<1..824)
                     /locus_tag="IV257_002856"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8815905.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFST"
     gene            complement(883..1344)
                     /locus_tag="IV257_002857"
     CDS             complement(883..1344)
                     /locus_tag="IV257_002857"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP8815906.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
BASE COUNT          553 a          297 c          193 g          544 t
ORIGIN      
        1 gttgaaaata atacagttgt tgttgctgaa gttacaattg caaaaatacc attaaccgtt
       61 gtcattgtag ttttgtatat tcccaaatag tatacgctta aaatgcctcc tacgataaaa
      121 gtacctatat aagatgacaa ccatgtacct aatgattcca ctagtgacca cgcactaaag
      181 gaaaacattt cgtaaaattc tttttttgaa aaaaaaagtt ttggtttcca ctctgataac
      241 ttagttaata ttgcagctgt aactaaattt cctattatag atccaattac tagtgaccaa
      301 taacttagtc caattatcgc caacggtact gtaataacta aaggtatcaa tgcagatatt
      361 attctagcat aacacaaaat tttgtaatca aaatttcgtc taaaaattgc cgattgtata
      421 ctggaaaaag aagtaattgg aagagaaact ccagctacta taattactaa tccaagtcca
      481 gaatttccaa ctaagtgcgc tattttatta ttaaacacac aaataaataa ccataataat
      541 aaagaaatac ctaagttact agtaaaagct acatttgttg atgaaaacat gtgttcttca
      601 ttatcaaatt catgctgtac taaaaatttt tgaaatcctg aatcactaaa catatcagcg
      661 aaactaataa ccatagttac agtggctact actccaaaag catctggagt caataatcta
      721 gctaaaatca tattcgaaat tggagttata atctttgcaa ttatttcagc taaggatgac
      781 cattttaaag cttttgctgt ctttccatga atattatttc ccatttatta ccccattttg
      841 ctaaataaat atagcaacct ttcgcatatt aaacattact ttttaatcaa atttataatc
      901 aaatttcaac tctgttatga ttgctggatt tccatatact aagctatatg gtggaacact
      961 ttttgtaact acagcatttg cacctatgat tgaatcatgt ccgatattaa ttggtcctaa
     1021 aacacatgca ccagcgaaaa cagccacatt ttcctctagc gttggtgccc caacagtttc
     1081 ttttccattt attattttgc catttccacc caaagtcaca ttttgatata ttttacaatt
     1141 attaccgatt tttgttttag ggtggaatac acaaccaaga ccattatgga ctagttgagc
     1201 attagagcta aaatctacct gtccgtagaa ttcacatcca taaataatcc ttaaaatata
     1261 gcaggatatt tttgacattt tattttgacc attcctgtat tgtctacaag ctttatctgc
     1321 ccatttctct gcttttcttc tcattttttc tacctcaact ttcttttttt atagttcttt
     1381 ctgatgcaga aatcatacac aaaacaacta ttgcgtaaag aaaacgcgaa gatacaatat
     1441 ccggtataaa cattgctccc actactatca tcaaaaataa ccatttatag caacctgatt
     1501 tctttaattc ttcatacgta aatttaaaga agccacttaa aataattagc gtaccaataa
     1561 ttcctgtctg taataaatat tgaataa
//
LOCUS       DACDIP010000171         1550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDIP010000171 DACDIP010000000
VERSION     DACDIP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.175"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..>1550
                     /locus_tag="IV257_002858"
     CDS             80..>1550
                     /locus_tag="IV257_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8815907.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHC"
BASE COUNT          536 a          217 c          320 g          477 t
ORIGIN      
        1 tttatattga caacggttac aatgggtttt aaaatataaa atgtatcaaa actaacatat
       61 cagtttgagg aggttaatta tggtttcgat taaaaattgg caaaattcta tcgaagaatt
      121 caatgaaatg cagattctac tgccgtgttt tgatgtcaaa gaagttaagc aagaaggcca
      181 gcaacatcca gtatggcttc attttggtgg aggaaatctt tatcgaggat ttcatgcaga
      241 aattgctcaa tgcttagcag atcaaaatga attagaaaca ggagtagttg tgtgtgaaac
      301 atttgatgaa gaagtagtgg aaaaagctta tcatgactat aacaatgaca ttttggaaat
      361 tgttatgagt ggagatggtg aactcaaaaa aagacttttg gcagtaacgg ctgatagttt
      421 atattgcaat ccgaagaata tagcggacta tcaaagagtg gttaaatatt ttgaagatcc
      481 taaattacag tttacaacgt ttactattac tgaaaaaggt tacagtttaa aagattctaa
      541 agggcaattc ttttcggtag tcaaagctga tatcaaatct ggacctagca atcctcaaca
      601 tacgatgagt attgtaacat ccttgttata tagcagattt aatgctggag agttgccgat
      661 tgcgatggtt tctactgata atttctcaca gaatggtcaa cgttttcaag atagtatttt
      721 tacgattgca gaagcttggc aaaaagaagg atatgtttct gaaaaattta ttgcttattt
      781 aaaggataaa gaaaaagtga cttttccttg gagtatgatt gatcgaatta cgccaaatcc
      841 ttcagagaat gtaaaggaag atctattgat gatggggctc gaggatttag atatcattca
      901 cacaaaaaaa cataccaata ttgctgcttt cactaatact gaggtagttc attatttagt
      961 tattgaagat agttttccaa atggccggcc taaattggaa aaggctggtg tgattatgac
     1021 agatagagag acggttgata aagcagacat catgaaggtt acgacaagct tgaatccact
     1081 tcatactgct ttggcagtaa caggttgtct gctaggatat caatcaattt cagcagagat
     1141 gcaggatgat gatttggttg gtttaatcaa aggaataggt tatttagaag gtttaccagt
     1201 agttgaaaat ccaggcatta ttgatccaaa acaatttatt gatgaagtaa ttaataaacg
     1261 tttgcctaat ccctatattc cagatacacc acaacgaatt gcaacagata cttctcaaaa
     1321 agtagcgatt cgctatggtg aaacaattaa aaagtatgtc gaaaaagacg gaaatgcaga
     1381 aaatctaaca tttattccta tcgctattgc tgcttggtta cgttatttac tggcagtcga
     1441 tgatcaaggg gaaaattttg tgccaagtcc tgatccgtta ttggtagagt tgcaggataa
     1501 attatcagta ttgaaattag gtgaacaaga tccttcaaag attcattgtg
//
LOCUS       DACDIP010000172         1516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDIP010000172 DACDIP010000000
VERSION     DACDIP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.176"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            290..697
                     /locus_tag="IV257_002859"
     CDS             290..697
                     /locus_tag="IV257_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815908.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            711..1112
                     /locus_tag="IV257_002860"
     CDS             711..1112
                     /locus_tag="IV257_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815909.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1114..1485
                     /locus_tag="IV257_002861"
     CDS             1114..1485
                     /locus_tag="IV257_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815910.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          492 t
ORIGIN      
        1 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
       61 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      121 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      181 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      241 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      301 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      361 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      421 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      481 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      541 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      601 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      661 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      721 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      781 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      841 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      901 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      961 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
     1021 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
     1081 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
     1141 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
     1201 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
     1261 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1321 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1381 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1441 ctgatgaatc gagaagcaga aaaaataata aacaaactca cctaatgaat ttcaagcgag
     1501 tttgtttgtt attttt
//
LOCUS       DACDIP010000173         1511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDIP010000173 DACDIP010000000
VERSION     DACDIP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.177"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..187)
                     /locus_tag="IV257_002862"
     CDS             complement(<1..187)
                     /locus_tag="IV257_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8815911.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVH"
     gene            complement(188..532)
                     /locus_tag="IV257_002863"
     CDS             complement(188..532)
                     /locus_tag="IV257_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8815912.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            882..1478
                     /locus_tag="IV257_002864"
     CDS             882..1478
                     /locus_tag="IV257_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8815913.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          411 a          268 c          257 g          575 t
ORIGIN      
        1 tatggacatg ccagtgattg cgacaacctg gttcaaaagt tacattacct acaccaacag
       61 aaacttcggg atcattcact aacatattca gatagctttg cccggtaaat acttgagcgt
      121 aagcttcgtt tttttcacct gctggaaaaa tcacgccatt gtgtatttct tcgtgttttg
      181 tcatcattta cgcctctttc ttatcttcgc tattttcttc taaatagatt tcttttgcta
      241 aactaaaagc tgaccaagct tttggccatt ctacataaaa agctaaatgg gtaatgattt
      301 ctgctatttc ttcactagta atgccatttt gcttgccaat cttcagatga gctggcaatt
      361 gttcaaaatt tcctcctgaa attaatgcag atatggtgat gatactacgc tgatgagcag
      421 ataattcttc tctggaccat acttgtccaa ataaaacatc atcatttaat tctgcaaatt
      481 ttggtgcaaa atctcctaat tgatctcgtc ctgcggtttg tttttctgcc atttctctta
      541 ttctcccttc acgactgtta ggtgattcaa ctatggttct aaggcaactt tattttctat
      601 tgtttcttcg catttcattt taaatgtagt ctttcagcct gtccaatgct tagtttattc
      661 agtcgatatg ctttttaagc atagttgcta acgctttttt tattttaaaa aaaacaagtt
      721 attaaataag tctttagttg gatgtttttt tgagatcaat cttttatact tatgatgaac
      781 ttacaaaatt gtttgttttc tgtcatatca aaaaatggcg cagttagttg cttttctagt
      841 aaagtatagt taacaaagaa cctctaagga gttgatgaat aatgaatatt gctgcctctc
      901 gaaaactgat aaatttcata tccattatcg gattaggcct atcaatcgcg ctgacgattt
      961 attttattaa tttaggtgtt ttcaaagatt tgaatgcgtt aagaggatta gtaggtgatt
     1021 ccatcatttt aggaccgatt atttttgtct tcatccaaat cctgcaagta gttattccaa
     1081 tcattcctgg agggatcagt actgcagctg gtgtcctgat atttggccct tatgctggat
     1141 ttatctataa ctatattgga atctgcattg gttcgattat catttttctg ctagggcgtc
     1201 gttatggaaa gccattcata ctttccatga ttagcgataa gacatataac aaatatattg
     1261 gctggctgga taatcaaaac cgttttgaga agctgtttgc tctagctatt tttcttccag
     1321 ttgctcctga cgatgcgtta tgtttgatgg ctggattgac aaacatgtca gtgaaacgat
     1381 atacactaat cattttgatt gctaaaccac tgtcgatttt cttatatagt atggccttga
     1441 tttatggagg tcaatatcta agtggtcttc ttggataata tttattttgt aaaattagtc
     1501 attgattgac a
//
LOCUS       DACDIP010000174         1499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDIP010000174 DACDIP010000000
VERSION     DACDIP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.178"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            218..550
                     /locus_tag="IV257_002865"
     CDS             218..550
                     /locus_tag="IV257_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8815914.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1169..>1499
                     /locus_tag="IV257_002866"
     CDS             1169..>1499
                     /locus_tag="IV257_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8815915.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVAD"
BASE COUNT          521 a          203 c          329 g          446 t
ORIGIN      
        1 aatacgtgat attacgatga atctatgcct aagcataatg ttataaaaat gtccgtgtgg
       61 ctctttgccg tatgacgaac gctgaaatta gagggaacgc atgtttggat gcgttccctc
      121 ttttttgttt gtcatgaacg aaaatcaatt ctctattttt gaaaaataac aagaaaaact
      181 tgttaaaaat atttaaagaa attgggtggt ttattttatg tcatatacag gaattttgag
      241 tctcaaagat atttgtcact acggtaaacg gtgtaccgct acagagaaaa tcacgaagaa
      301 actatcaact ggccaaagta aaaccgttgt gcagtgtaag aaatatataa tccagaaaga
      361 caaagtctca gaggaaatga tttattacat tgggaaacaa aagcaaataa ttttaaaaga
      421 tcctatcccc ttaaaagagc tatatccaac aatcaaacat gtttatgacc aaaacggtgt
      481 actgattggt cgaagaaaaa atggagtctt gcgttgtacc gcaaaaggga tggggcgttt
      541 gatcggctga attgatcatt ctcataactt gtattttatc gtttttcgct tgcttgaaaa
      601 tattgtcgag acttctaaag gaacttagag tcactagaaa aataaggagt agacttgatc
      661 tatcaacaat tcccttcggg gagtgacggg gtaggtttta acgattggta acgtaaaaaa
      721 ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga aatgaatttg tgtaaataat
      781 aagcgcagag taaacaagag ggaatttaga agttagacaa gttagaatag cgtctagatt
      841 aatcatgagc agctactaag ggatctagac tttgacgcaa actcatggaa taaggagtag
      901 gtcttctcgt aatggggagg acgcacaaga aattgtgtcg tgtgctgcag tacatgtaga
      961 tacttgcgag gacgttgatt attggatcaa tcgagcattt accgctgtct actagggttt
     1021 ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt atttttaagt aaacgaaaaa
     1081 cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt aatataaagc atagagaaaa
     1141 tgaaatttac gtaatggagg aatgacaaat gaaaaattat gatccgaata tacgattggg
     1201 cacacatact attaaggttt ctttccaacg ttgggattac aaaggatttg tgacgtttag
     1261 aaggggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg atttatacga
     1321 tcaaacattg actgataatc caattggatt cggcttgcta cctgaagatg atgaaggtaa
     1381 tgaatggttt aaaatgacgc ttatgaatga taatggagac gaattatctg ttgaagatac
     1441 ttggtcatat ttgagtgatt atattgtcag ctttgaaatc attgaatttg tagcggata
//
LOCUS       DACDIP010000175         1490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDIP010000175 DACDIP010000000
VERSION     DACDIP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.179"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            196..921
                     /locus_tag="IV257_002867"
     CDS             196..921
                     /locus_tag="IV257_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815916.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IV257_002868"
     CDS             911..1420
                     /locus_tag="IV257_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815917.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
BASE COUNT          409 a          288 c          242 g          551 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt
//
LOCUS       DACDIP010000176         1484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDIP010000176 DACDIP010000000
VERSION     DACDIP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.180"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(23..309)
                     /locus_tag="IV257_002869"
                     /pseudo
     CDS             complement(23..309)
                     /locus_tag="IV257_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(299..628)
                     /locus_tag="IV257_002870"
     CDS             complement(299..628)
                     /locus_tag="IV257_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8815918.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(705..977)
                     /locus_tag="IV257_002871"
     CDS             complement(705..977)
                     /locus_tag="IV257_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8815919.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(977..1240)
                     /locus_tag="IV257_002872"
     CDS             complement(977..1240)
                     /locus_tag="IV257_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8815920.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT          457 a          295 c          184 g          548 t
ORIGIN      
        1 tcttaagaac gacaacaaat cactagatta attttttcaa ctgttcataa aagttttcat
       61 gcgttccaca taacaatact ggaataacct tgttttctat aatctcgtaa gctactctat
      121 atgttgtgcc cgcatatttg aacccactag accaaatacc tgataaatcc ccagtttttt
      181 gttcaccctt atatggatca atcgcaattt catcataaat taaattcaaa aatttttctt
      241 tcaatggctt ctcttttagc tttttaaata cttaatgaca tgttttgaag gaacgatttc
      301 ataagccaat ttctgttgcc aactcctctt tagtcattac tttagaatta gtaagtgtat
      361 cttcagcaat atctctaaag gaagaatgca ttttgttttt tctattctta aattctacaa
      421 gaatctcatc tttatcgtag ccttctgcaa ttatatcaga caaaatatct tcgctaaaat
      481 cataaaattc tttttgtggt tcaacaaatt cataaagaat accattctct accaactttg
      541 cttcaacttc gactccatta ggaacattaa aatctttagg tactgtcaac atgattgaat
      601 tgccttgttt tcttgttttt gtaatcattt aaatccctca tttctaacag tatgtaatta
      661 cttagtaaat acattatacc acgagataat gtatttacaa gtaattactg tgactcgtaa
      721 tgcgtccttg ctgaataaat ttcaaccacg tgttcatctt catctacctt ataaacaacc
      781 cggtgttgtc tattcaatct tctagaatac ctaccttcat gtgtaggttt taacttctca
      841 aatgattggg tagggagata gggatcttcc tttaaagtct gaataacctt ctcaaattga
      901 ggtttcaaat tagactgttt gattttcttt aaatcagctt tagccgagtt tttaatcatc
      961 acagaatagt tattcattat aagttatccc aatcaatttc atcaacatta gtaaatccac
     1021 tatcatcttt ttctcgctta cgaacaacac ccaaagtgcc tatttgctca agtaatagcg
     1081 tttctttgat tgctttccaa tcatccaaac caataagcac cgcattattt tcactgcgct
     1141 cactaatgat ttcaatttcc gtatgattct catttacatc tttcaacaac ttataaaaat
     1201 ttttccgtgc tgagctagga tttaagactg tttttgccat atctactccc cctctaattt
     1261 atatctccat tctaacgtac ttaattaagt acgtaaattc gattgttttg tttaaattgg
     1321 agtaccaaaa cagcattgta aagtgttatc cgtttccttt atacccgtgg tgctagttaa
     1381 ttttttccta gaggaaaatg tctaaacatg ttggtaaatc aacaaaaaaa cgcatagagt
     1441 agtctcaaaa ctcccaagaa atgaggacta catatgcgtt catc
//
LOCUS       DACDIP010000177         1413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDIP010000177 DACDIP010000000
VERSION     DACDIP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.181"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            88..1380
                     /locus_tag="IV257_002873"
     CDS             88..1380
                     /locus_tag="IV257_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8815921.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          459 a          288 c          256 g          410 t
ORIGIN      
        1 cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga attaatttgg
       61 cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa agaaacatta
      121 gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga aaaatacaaa
      181 gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga atgcatccac
      241 tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt ccggttgtta
      301 atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg cttcaaatgc
      361 aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca ttgtttcatc
      421 agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc aatgacagag
      481 atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa gcgattttat
      541 cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga tgaattcaaa
      601 tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca aacacagcgc
      661 ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt tagtcatttt
      721 tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc tccttatgta
      781 tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt tcacatcgtc
      841 caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca actattaaaa
      901 agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg gaaacttctt
      961 caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg cttcaaagct
     1021 catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta
     1081 ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta cgtatcgttt
     1141 gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac cttgagaacg
     1201 ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc aaatggccca
     1261 cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg ctttcgtagc
     1321 ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat tttcaactaa
     1381 aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DACDIP010000178         1388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDIP010000178 DACDIP010000000
VERSION     DACDIP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.182"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            597..1070
                     /locus_tag="IV257_002874"
     CDS             597..1070
                     /locus_tag="IV257_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8815922.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1079..1306
                     /locus_tag="IV257_002875"
     CDS             1079..1306
                     /locus_tag="IV257_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8815923.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          473 a          227 c          210 g          478 t
ORIGIN      
        1 tactttttat tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt
       61 tgatttataa actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag
      121 taccacccca tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag
      181 gacaaataag tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa
      241 acatgaactt gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat
      301 acagttctgt taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg
      361 tccaatataa tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa
      421 ataactggtt ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt
      481 catactctat cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg
      541 ttttacaata attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga
      601 aatttaaaac attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt
      661 tcggcgtact tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc
      721 agaccacatt atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt
      781 atttcttatt gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct
      841 taaacatact aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct
      901 tacttcctaa atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag
      961 gaatcgctgt cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac
     1021 tgcttgaaca tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat
     1081 ggcaattatc gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt
     1141 gagtgagaaa gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa
     1201 agccatccgt ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga
     1261 tttgttgacc tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca
     1321 aacagtaaat aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg
     1381 tctattat
//
LOCUS       DACDIP010000179         1377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDIP010000179 DACDIP010000000
VERSION     DACDIP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.183"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(680..753)
                     /locus_tag="IV257_002876"
     tRNA            complement(680..753)
                     /locus_tag="IV257_002876"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(717..719),aa:Ile,seq:cat)
     gene            complement(943..>1377)
                     /locus_tag="IV257_002877"
     CDS             complement(943..>1377)
                     /locus_tag="IV257_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8815924.1"
                     /translation="TDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENA
                     NLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFN
                     MRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          426 a          251 c          241 g          459 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcatga ctgacgtgcc ggacgcacgt agcgatcatc ggcgttatga
      121 ctatataaag tatactttcg ttgatccaga aaggtataaa gattaaagta ggctacctaa
      181 aaaggtagcc cggaacgaat tttatcacca tacttgtgaa aggagatatt ttttaagtta
      241 gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc tataaaatca
      301 aaaataagtc actaattaac taccactcca attgtaagcc ttttttctca ctttttttca
      361 aaaatatggt atgcttttta atggcttcaa atataaaaga gtttaaagcg taacacactt
      421 atggggaagt ggtttggggt gcgctttaaa ctcttcttta ttattatctc acaatttaac
      481 ccaaatgtct ttctatttaa aaatcaaagt aaaacttttc aaatatacag aagtataact
      541 atgtgaaaca tcctttcatt aatccataaa aggatacata aaaaagccac tcatttgagt
      601 ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaaattcta acacagaacg
      661 attcaaatgg ctacgttaat gtaccctgta ggactcgaac ctacgaccgg acggttatga
      721 gccgtctgct ctgaccaact gagctaaggg tactggttgt tgccacataa agccataaac
      781 aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc atagcttgga gtgtgactat
      841 ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaataa tttttatagt
      901 tgtcaatata gttatgtact gctcctcaac gaggagctat ttttatcgtt taggaatatt
      961 taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc
     1021 attcgttgcc cgcatcatta catagacttt cttactagga aaattacgca tattgaaaga
     1081 tacatgataa ccaacatttc cagaagtatt ataagcttgg tttacatctg gtctataaat
     1141 tccatcagct cttactcgag ctaattcttt cccagtattg tagtccataa tgaaaatata
     1201 ctcgtattta tagttagcaa tgtgccatcc agccacatgc aagtttgcgt tttcgatttc
     1261 cccaaactga tcaatgtggg cgtgatttgt tccatctgtc agcgtaggat tagctgcacc
     1321 agctcgtgtt ggatcaatga caggcttgtt ttcagaagtt gttggatttt catcggt
//
LOCUS       DACDIP010000180         1350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDIP010000180 DACDIP010000000
VERSION     DACDIP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.184"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..265)
                     /locus_tag="IV257_002878"
     CDS             complement(<1..265)
                     /locus_tag="IV257_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8815925.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRM"
     gene            complement(396..980)
                     /locus_tag="IV257_002879"
     CDS             complement(396..980)
                     /locus_tag="IV257_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8815926.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          494 a          232 c          163 g          461 t
ORIGIN      
        1 acattcgaaa tacaatatat aaggatgtcc caaaaaatat agtaaaaaac tctatcttga
       61 tagcatcaat aattttcatt actaaaaaca gaactgaata aatcaagatg aaataaatac
      121 atgtctttat tttacgaaac atcaatacta aaactgtagc aataatgaaa caccccactt
      181 gaaatatatt tccctttcct aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca
      241 aggttaacgg attgattttt gacactcttt cacctccaaa aaataagatc gcactgttat
      301 cttaagctga taaaaatgca ccgttattca gataacagtg cgatcttatc aaaaagcata
      361 actgagaaca attctcaatg caagcttttt tttaattaac tcagtaattc aatccaccca
      421 ggaacagaaa attgatacaa tcttctggtt aatagcatag tcttttcttt tgaatagcta
      481 tgcttcaata tatcatagaa tgtagcgtag aatgagtaaa tgtagaagtg gatttcttct
      541 tcattaaaga ctaaattttg attgatcatt ccgatagtct ttaacttatc tagacaacag
      601 actgtctttt ttgtcacaaa tgatgtaatt ttttcaacaa tatcttgata tttggttcca
      661 gctgattgga ataaaaccaa atcgaatata cgtttattct caaaaatata attaataaat
      721 ggatctaatt cattgaagtt ctctctccaa cttttctcta tatctatatt ttctaagttt
      781 aagaggtatt tggtaaactc ttcattatac atattaataa gcgcattaat atgcggctga
      841 ataatttctt caaataacgc ttccttagaa gtaaaatgtt tataaaaagc acccgtagtt
      901 acgcctgcgg tcaagcacag ttgccttatc ttagtttgct gaaatcctac tttttcaaaa
      961 tggcttagtc cactttctat taatttttgg tgagttgtaa tataatctct ggtttctatg
     1021 ggtacttcct cctaataaga tgaattaact atattatctt acacaggact tgaagtttca
     1081 atcgttaacg tctattactg aactcattcc ttagagctaa actaatgaga aagagcaaaa
     1141 taactgatac aaaaagcaat cctacaagaa ttaatagact catctaaatc attcaccttt
     1201 tctaaaataa tccgagaaag atttaaaaaa attatatcca gctattagga taagtcctat
     1261 cataatcact ccaatagcca ttttcttatc ctccctaaaa ataaagcctt ttacttaaaa
     1321 ccttttttgt aatcgtcaat aaattttttc
//
LOCUS       DACDIP010000181         1343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDIP010000181 DACDIP010000000
VERSION     DACDIP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.185"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(69..1208)
                     /locus_tag="IV257_002880"
     CDS             complement(69..1208)
                     /locus_tag="IV257_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8815927.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          351 a          289 c          249 g          454 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtttatta
       61 aaaaaatctt acgctaagta ggataaaata attccttgat atttttcgat catatccaaa
      121 taattatcga tttcaacata ctcatctact tgatgaggca aagtaacgac accaggccca
      181 aagacgacaa agtcgaagga atggttagct ttcgtaaatt cggctgcatc cgttgttgct
      241 gccgctccaa ctaaaggtaa tggctggctg aattgttgct gaatgcaatg aatcaaagga
      301 gaatctggat cagctttgac aggtattttg ttgtaatcga tcatcaattc caaatgatag
      361 tctgtctctt gattcaattc attgacgatt gattgcagca atgcaatgat tttatcattt
      421 gggtattctg gaatcgatcg aatgttccct tggagctgcg catggcttgg gatgctgttg
      481 acttgattcc cgccactgat caatgtaaca ttatggattg tttttcctag gacaggattc
      541 tcgattgttt cagcgaggtg attcatttca gcatttgctt tcgtaatgaa ttcatttagg
      601 tgattgattg cattataacc ttggtctggc atagagctat gcgcttcttt tccgtgcgag
      661 gtgactgtat agttgattga tcccatgtgc gtatacatca aactgtaatt ggttggctct
      721 ccgatgatca aggcatctaa gtcatctaca tagcctgctt tcgtgagctg ttccccacct
      781 agttcaccca cttcttctcc gacagtggct aataatttga ctgttccgtt aaatggtttt
      841 cctgactctt tcagttcgat catggcaatc accattgctg ccaagccact cttcatatct
      901 gtagcaccac gtccgtacaa acggttccca tggatttctg ctgcaaaggg tgcataggtc
      961 caagacgatt catcaccagc cgccacgaca tccatatgcc cagataggcc taacactttt
     1021 cctgattggc ccttttggta acgagcaatc aaattatctc taccatctct gtaagaaaca
     1081 atctctcctg ttatgtcatg ctttgccaaa agcttattca aataggctgc tacttcacct
     1141 tcgtttccat tcactgattt aattcggata atctcttgta aaatcgcgat tttttcttct
     1201 tttttcatct aatcctctcc cttgccattt ctttttattg tatttgagtg tgctcgaatt
     1261 ttcaaatagg agcaaccaaa gtaaaaatga agaaagagag ccactttatg aaaaatatgc
     1321 tgctacttcc ctcctatttt att
//
LOCUS       DACDIP010000182         1312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDIP010000182 DACDIP010000000
VERSION     DACDIP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.186"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(156..1297)
                     /locus_tag="IV257_002881"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="IV257_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8815928.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          323 a          263 c          278 g          448 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc tt
//
LOCUS       DACDIP010000183         1262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDIP010000183 DACDIP010000000
VERSION     DACDIP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.187"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            70..408
                     /locus_tag="IV257_002882"
     CDS             70..408
                     /locus_tag="IV257_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8815929.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            500..1241
                     /locus_tag="IV257_002883"
                     /pseudo
     CDS             500..1241
                     /locus_tag="IV257_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          479 a          201 c          257 g          325 t
ORIGIN      
        1 aaaatgacaa attgatgaat caaattcaaa ttaagggggg caaccctgga agtcttaaat
       61 ttaccctcaa tgaatggtat tgaagatgaa aatttaaggc gtttgattaa tagccttgtc
      121 attgaattgt acaagtatca agcagaatca gaacgaaaaa aaattaagga acgtcaggca
      181 caaggaatcg aaattgctaa gaaaaaaggc aaattcaaag gtcggcaact gaaatttaaa
      241 aaaaatgatc cacgtttaaa gcatgctttc gatttgtttt tgaatggttt atccgataaa
      301 gaagttgaag aacaaactgg gatcaatcgc cgaacgttta gaaggtatcg agcaagatat
      361 aacgtgacag tagatcaaag aaaaaacaat gaaaagaggg atagttaatg agtacggtta
      421 ttttagctga aaaacaagcc aggcattagc ctacgcaagt gctttaaaac aaagcaccaa
      481 aaaagacggt tattttgaga tcaaagaccc actatttaca gatgaaacgt ttatcacctt
      541 tgattttggg catttagtgg aattagcaga accaggtcat tatgacgaaa agtggcaaaa
      601 ttggaaactt gaatctttgc cgatttttcc tgatcgatac gattttgaag ttgcaaaaga
      661 taagggaaag cagtttaaaa ttgttgcaga acttctcaaa aaggcaaata caattattgt
      721 tgcaacagat agcgacagag agggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa
      781 tgccttttca aaagataaaa catttaaaag actatggatc aatagcttag aaaaagatgt
      841 gatccgaagc ggttttcaaa atttgcaacc aggaatgaat tactatccct tttatcaaga
      901 agcgcaaaca cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttgtatac
      961 gttaaattta caacagaagg gcgtacaagg tacattttca ctaggacgtg ttcaaacgcc
     1021 caccttatac cttatttttc agcgccagga agctagtata aaagtaaaac aagggtcatt
     1081 taagggcgtt ctaagcccca cacagcgttt taaaacccaa gaggagcttt tttgcttttg
     1141 tttcttctaa acaagctaaa ataggcaatc aagaggggat aattgctgat gttcaaacca
     1201 aagagatgga taggctacaa ttaactagac agaaaaatta aggtgtgtag actagaagaa
     1261 aa
//
LOCUS       DACDIP010000184         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDIP010000184 DACDIP010000000
VERSION     DACDIP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.188"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IV257_002884"
     CDS             200..742
                     /locus_tag="IV257_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815930.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IV257_002885"
     CDS             832..>1246
                     /locus_tag="IV257_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8815931.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDIP010000185         1237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDIP010000185 DACDIP010000000
VERSION     DACDIP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.189"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1237
                     /locus_tag="IV257_002886"
     CDS             <1..>1237
                     /locus_tag="IV257_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8815932.1"
                     /translation="LGDAMLTPSLQDAHIALYDIDEKRLKESNLMLKNINKNSNENRA
                     DIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKKYGLRQTIGDTMGIGGIFR
                     TLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLKATSIKTVGLCHSVQVCVP
                     ELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDFYPTIRQLAAEKPNPHPDS
                     VRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELGIPLDEYLRRCEQQINDWE
                     SMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNVLNKGLITNLPTDVCVEVP
                     CLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALTLDKSKIYQAAYLDPHLSS
                     ELSLTDIKNLVDDLIEAHGD"
BASE COUNT          428 a          192 c          247 g          370 t
ORIGIN      
        1 ttaggcgatg cgatgttaac tccaagtcta caagatgcac atattgcatt gtacgatatt
       61 gatgaaaaac gactaaaaga atcaaattta atgcttaaaa atattaataa aaattcgaat
      121 gaaaatcgtg ctgatattgt ttcttattat gatcgcaaag aagcgttaag agaggctgat
      181 tttgttatca atgctattca agtgggtggt tataaacctt caacagtgat tgattttgat
      241 attcctaaaa aatatgggtt gcgacaaact attggtgaca ccatggggat tggtgggatt
      301 tttagaaccc tacggacact accagtaatg ttagactttg ctaaagaaat ggaagaagtt
      361 tgtccaaatg cttggttatt gaactataca aatccgatgg cgatgttaac catgggtgtt
      421 ttaaaagcga cgagtattaa gactgttggt ttatgtcata gcgttcaagt atgcgtacca
      481 gaattgtttg agcatttagg aatagccgat caatatgact taaatgactt tcaatggaaa
      541 attgcaggca ttaatcatat ggcattcttg ttggaaatta caaaaaatgg ggaagatttc
      601 tatccaacaa ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga ttctgtacgt
      661 tttgaattgt tgaaacgttt tggttactat attacagaat caagtgaaca caatgcagaa
      721 taccatccat actttattaa atcaaaatac ccagaattga ttgacgaatt agggattccg
      781 ttggatgaat acttacgtcg ttgtgaacaa caaattaatg attgggaaag catgcgagag
      841 gacattgtaa ataatgggga gttaacacat acgagaagcc gtgaatatgc ttcatatatc
      901 atggattcta ttacgacagg aactccgtca gtgattgcag gaaatgtctt aaataaaggc
      961 ttaatcacaa acttaccgac agatgtttgt gtggaagtac cttgtttagt cgataaaaat
     1021 ggcgtgcaac caacctatgt cggtgaattg ccaacacaat tagctgcttt aaatcggacg
     1081 aatatcaatg ttcaagaact gacagtagaa gccgctttaa cactagataa atcaaaaatt
     1141 tatcaagcag cgtatttaga tccacatcta tcatctgaat tgtctttaac agatatcaaa
     1201 aatttagtgg atgatttaat cgaagcacat ggggact
//
LOCUS       DACDIP010000186         1235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDIP010000186 DACDIP010000000
VERSION     DACDIP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.190"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..115
                     /locus_tag="IV257_002887"
     CDS             <1..115
                     /locus_tag="IV257_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP8815933.1"
                     /translation="RVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            291..1034
                     /locus_tag="IV257_002888"
     CDS             291..1034
                     /locus_tag="IV257_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8815934.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1027..>1235
                     /locus_tag="IV257_002889"
     CDS             1027..>1235
                     /locus_tag="IV257_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8815935.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGF"
BASE COUNT          448 a          178 c          250 g          359 t
ORIGIN      
        1 cagagtaaac gaaatagtaa catttgcaaa tggagaacaa ctagagatgg gaaagagaag
       61 tattgataaa ctaaagaatt ttatgcaaga aaagatagaa aatgaatcat tttaaagaaa
      121 acaaagactc atatcttacc tgttagaaga gtgttttcct gtagaagaga acaacccaag
      181 tagcagccta gtcgtttcta tccttttttg ttatgcactc tttttgacat ttactccacc
      241 aataacgaag ggtacaataa aaagtaataa caaacaaaag gagtacagaa atgattgaat
      301 tcatcaatgt aagtaaaagt tacgggaaaa aacaagcatt aaaagacttg tctctctcga
      361 taagacaagg ggaaattttt ggatttttag gacataatgg tgccggaaaa tcaactacga
      421 ttaaaagttt agttagtatt attgaaccat catcgggaac gattttagta gatggaatga
      481 aattgacgga aaatcgattg agtatcaaac aaaaaatcgg ttatgtgcct gattccccag
      541 atattttttt acaattgacg gctggagaat attgggactt gatttcagct gcctatgaat
      601 tagacacaca aaaaaaagaa aagcgtttag cagaattgac agcgcttttt gatatgtata
      661 gtcatcaaaa tgaaacaatc gcaagttttt cacatggaat gcgtcaaaaa acgattttga
      721 tcggcacgtt gctgccagat ccagatattt gggtattgga tgaacctttg caaggacttg
      781 atcctcaagc agcatttgat ttgaaagaaa tgatgaaggc tcatgccgca aaaggaaaaa
      841 cagtcatttt ttctacacat gtattggata ccgcacaaca gttatgcgat aaactggcta
      901 ttttgaagaa aggtgaattg atttatcagg gggcagttag tgaattattg aatggctcac
      961 ctgatgaaac gctagaaaag atttatttga aaatggctgg aaggcaagcg acaggagatg
     1021 gaccgtatgt ataagcgaca attattagaa ttaattcgag ttaatctttt atatgttaat
     1081 ccacagctca cgaataaagc aagagagaaa ggaaaaaagg gaaagcgatt aactatctat
     1141 cttttgtctc aaaatctttt ttcagggctg atttttttac tgatttacgg atttgctatg
     1201 tttgctatga actttgttaa attgccaggt ttctt
//
LOCUS       DACDIP010000187         1218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDIP010000187 DACDIP010000000
VERSION     DACDIP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.191"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            97..1176
                     /gene="uxuA"
                     /locus_tag="IV257_002890"
     CDS             97..1176
                     /gene="uxuA"
                     /locus_tag="IV257_002890"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8815936.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
BASE COUNT          415 a          166 c          261 g          376 t
ORIGIN      
        1 gaaaactgaa aaaatctttt gcgaaatgct agtaggtcct caagcagttc gtaaaacaat
       61 caataaatat ataacagaag ttggaggaaa gtaactatgg aaatgacttt tagatggtat
      121 ggggcaaatg aagaaaaaat ctcactacaa gaaattcgtc agatcccagc aatgaaagga
      181 attgttggaa cattaatgga tataccagcc ggagaggttt ggccgagaga acgaattaaa
      241 tctttaaaag atgaagtcga agccaatgga cttaccttga aagttattga gagtgttaat
      301 gttcatgatg atatcaaaat tggtttacca acacgagatc aatacattga gaattataaa
      361 cagacgattc gcaatttggc agaatttggg attgaggtta tctgttataa ttttatgcca
      421 gtatttgatt gggtaaaatc ggatttggat tatcgtttag atgatgggtc ttctacgtta
      481 gcctatattt caaaaaatat ccctactgat ccggaggaaa ttattagaga agtagaaaat
      541 tctactaata attttgaatt gccgggttgg gaacctcatc ggttatcaca aattaagcaa
      601 ttattagaag cctataaaga agttgatgaa gagaaattac gagagaactt tgcatatttt
      661 ctcaaagcaa ttatcccgac atgtgaagaa gttgggatta aaatggcagt tcacccagat
      721 gatccaccat attccatttt cggattacct cgtattgtta aaaatcgtga cgatttagat
      781 tggttgtgta atgtagtgga ttctccttct aatggtatta cattatgtac tggaagtatt
      841 gcagaacgac cagataatga tgtctatgaa attctatctg aattttgtca acgggatcgg
      901 attccttttg cacatgttcg taatatcaaa tatttaagtg acaatggact cgatttttac
      961 gaagcgccgc ataaatcaga atatggttct ctagatatgt ataagatatt aaaaacaatg
     1021 tacgataatg gatttaatgg ctatattcgt ccagatcatg gtcggatgat ttggggagaa
     1081 accggtagac ctggttatgg attatatgac cgagctctag gtgcagttta tttaaatggt
     1141 atttgggaag cgcttgaaaa gggggcgagt aaataattat ataagggagt ttatttagag
     1201 gaaaaatttt ctcttcta
//
LOCUS       DACDIP010000188         1206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDIP010000188 DACDIP010000000
VERSION     DACDIP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.192"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            278..670
                     /locus_tag="IV257_002891"
     CDS             278..670
                     /locus_tag="IV257_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815937.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(843..1025)
                     /locus_tag="IV257_002892"
                     /pseudo
     CDS             complement(843..1025)
                     /locus_tag="IV257_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          437 a          142 c          187 g          440 t
ORIGIN      
        1 cgcttattat tgatatgaaa atttcgattt gacaaaaggt accgaataat tttgtaatat
       61 gaattattat caagaagtaa ttatgatgat aacatatgga aactagttag taataataac
      121 aggtgattaa acttttgaac gacttgtaat tctatatttt ttcttttaag taaaaaatat
      181 aggtgatttt ttttgacttc atatttataa taaagacacg taaatcaatt atttttcatt
      241 ttactttaac taataatgtt tatttggagg gattattatg ttagatgata gattgacgtg
      301 ttctaattgt ggtacattat tttactatga ggaagttaga aatattgtaa aacatagaaa
      361 aaaagaaatg cctatacgtt gtccaaattg caatgaagtt attaagcgaa agttgtctca
      421 tggttatttt gtcagctacg aactatatga agaaagtaaa ataaatctga aaaaaaaacc
      481 aaaatactat aaaaatttga aaactggagc tattataact gaaaaagaat ttaaaaattt
      541 agtgactgaa gaagaaagca ggttttataa tttgtctggt aatatttgcc ctggatttat
      601 ggatcagatt attgaaaatg agtcaattga atcaattgaa aactatgttc ctgttgactt
      661 accaaaatag aaaaaataat cctgttttta cagtgaatta ttttaatttt gattttcaat
      721 aaaaatatat tacaataata atcgtaatag ctaatgaaac aatttctcaa tctttttacc
      781 actccaaaca gggagcgtca ataattttgt gtaaataact cgtccttctg caaaataatg
      841 ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt
      901 ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat
      961 tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt attgaaagat
     1021 tcgattgtag aaccagaagg aatggagaac acttttgaac tttacaacaa aactactgtt
     1081 gataaattaa ttccaatttt agttccaact gatttaaatg gtcatgatgt tttacttcgt
     1141 tttaacggat ttttaggtga ggattataga tacctaaaaa tttctgatcc tcaaccttta
     1201 acagaa
//
LOCUS       DACDIP010000189         1203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDIP010000189 DACDIP010000000
VERSION     DACDIP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.193"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..821
                     /locus_tag="IV257_002893"
     CDS             <1..821
                     /locus_tag="IV257_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8815938.1"
                     /translation="RYKNIACKLKESICKQMTIDGPFGEQFLEGIGDEEKTTYSVKHY
                     QKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDHLYQNTMAFSAS
                     SFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNPQAFVTRLEELICLADLDGSWW
                     WWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQYFGLKFKKGVLQIDPVDNLTFS
                     WKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILHVEKGKTIHIQRRKR"
     gene            818..>1203
                     /locus_tag="IV257_002894"
     CDS             818..>1203
                     /locus_tag="IV257_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8815939.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPT"
BASE COUNT          372 a          200 c          282 g          349 t
ORIGIN      
        1 agcgatacaa aaacattgcc tgtaaactaa aggaatcaat ttgtaaacaa atgactatcg
       61 atggtccttt tggtgagcaa tttttggagg gaatcggtga tgaggagaaa acaacctatt
      121 cagtgaagca ctatcaaaaa ccgattcttg agcaagggtt aatcttttta agtgatgtga
      181 ttgtggatgg aaaaattaat ttattgatgc atgatggaga agaatcagac actacgttga
      241 tgccttttta tcaattttta gataataaag atcatttata tcagaacacc atggcctttt
      301 ctgctagttc tttcaatcca acctatagcg aggaaatcaa aggcatcact tggggattag
      361 aatccggcgc cacttttcct ggctttatca ctgttttaat gagcgagctg cacaaccctc
      421 aagctttcgt aacccgatta gaagaactaa tttgtttggc agatttagac ggttcgtggt
      481 ggtggtggcc gtatcgctta gaggctcaac aaggagaagt cgtgcgagac tttggctgcg
      541 gcaaatgcgg ctgggcatcg ggtctttttg tcagcctaat gatcagtcaa tatttcggat
      601 tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg ttttcttgga
      661 agaatttgaa gttcggtcaa gccttcattt cgattgaatg tacgcaatcc gagttgagta
      721 tttcaaattt gtcagaaaag ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag
      781 aaaagggaaa aacgattcac attcaaagga gaaaaagatg agtatactaa aaaatattac
      841 ccaatttaaa aaaatccaga gtcgagcaat cactcccgaa aatccaacag gcgagaaagg
      901 taaagctgcg atgttgccaa gcgagctagg accaagccgc aaaggtcgtg gcgcaattcc
      961 gctgacttct ggtgaagaaa ctgtgattgc ggatattact ggaacgggaa taattcgtca
     1021 tatgtggatg acgattcgtg atcgaacaga atttggcagt tttgttttac gtgatttaat
     1081 cttaagaatt tattgggatg ggtcggatag ccctgctgtt gaaagtcctt taggggattt
     1141 cttttgtaat gggtttggag agcgttgcga ggtgaactcg ttgccgatag tggttaatcc
     1201 tac
//
LOCUS       DACDIP010000190         1203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDIP010000190 DACDIP010000000
VERSION     DACDIP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.194"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..821
                     /locus_tag="IV257_002895"
     CDS             <1..821
                     /locus_tag="IV257_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8815940.1"
                     /translation="RYKDIACKLKESICKQMTIDGTFGEQFLEGIGDEEKTTYSVKNY
                     QKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDPLYQNTMTFSAS
                     SFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNHQAFVTRLEELICLADLDGSWW
                     WWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQYFGLKFKKGVLQIDPVDNLTFS
                     WKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILHVEKGKTIYIQRRKR"
     gene            818..>1203
                     /locus_tag="IV257_002896"
     CDS             818..>1203
                     /locus_tag="IV257_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8815941.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPT"
BASE COUNT          375 a          194 c          285 g          349 t
ORIGIN      
        1 agcgatacaa agacatcgcc tgtaaactaa aggaatcaat ttgtaaacaa atgaccatag
       61 atggtacttt tggggagcaa tttttggagg ggatcggtga tgaggagaaa acaacctatt
      121 cagtgaagaa ctatcaaaaa ccgattcttg agcaagggtt aatcttttta agtgatgtga
      181 ttgtggatgg gaaaatcaat ttattgatgc atgatggaga agaatcagac actacgttga
      241 tgccttttta tcaattttta gataataaag atcctttata tcagaacacc atgacctttt
      301 ctgctagttc tttcaatcca acttatagcg aggaaatcaa aggcatcact tggggattag
      361 aatccggcgc cacttttcct ggctttatca ctgttttaat gagtgacatg cacaaccatc
      421 aagccttcgt aacccgatta gaggagctga tttgtttggc agatttagac ggttcatggt
      481 ggtggtggcc gtatcgttta gaggctaaac aaggagaagt tgtgcgagac tttggctgcg
      541 gcaaatgcgg ctgggcatcg ggcctttttg tcagtataat gatcagtcaa tatttcggat
      601 tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg ttttcttgga
      661 aaaatttgaa gttcggtcaa gccttcattt cgattgaatg tacgcaatcc gagttgagta
      721 tttcaaattt gtcagaaaag ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag
      781 aaaagggaaa aacgatttac attcaaagga gaaaaagatg agtatactaa aaaatattac
      841 ccaatttaaa aaaatccaga gtcgagcaat cactcccgaa aatccaacag gcgagaaagg
      901 taaagctgcg atgttgccaa gcgagctagg accaagccgc aaaggtcgtg gcgcaattcc
      961 gctgacttct ggtgaagaaa ctgtgattgc ggatattact ggaacgggaa taattcgtca
     1021 tatgtggatg acgattcgtg atcgaacaga atttggcagt tttgttttac gtgatttaat
     1081 cttaagaatt tattgggatg ggtcggatag cccggctgtt gaaagtcctt taggggattt
     1141 cttttgtaat gggtttggag agcgttgcga ggtgaactcg ttgcctatag tggttaatcc
     1201 tac
//
LOCUS       DACDIP010000191         1202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDIP010000191 DACDIP010000000
VERSION     DACDIP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.195"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..667)
                     /locus_tag="IV257_002897"
     CDS             complement(<1..667)
                     /locus_tag="IV257_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8815942.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIY"
     gene            complement(826..1092)
                     /locus_tag="IV257_002898"
     CDS             complement(826..1092)
                     /locus_tag="IV257_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8815943.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1082..>1202)
                     /locus_tag="IV257_002899"
     CDS             complement(1082..>1202)
                     /locus_tag="IV257_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8815944.1"
                     /translation="VVNGRYVITHGFTKKTEKTPISELKHAKELREEWFTNDD"
BASE COUNT          333 a          234 c          154 g          481 t
ORIGIN      
        1 catatattag cagcaatctt tcatcattgt caaattctat tggatcaaaa atatctaagt
       61 tgtcatcaaa atctttatca gttccggtaa cttttttgtt aattactgaa ggtgtcttgt
      121 aagaaataga atctaaatca aagcgttttg attctaaatt attttgtgct ttctcaatta
      181 aataattcac tttttttgtg agaaaccttc tgatattgtc tttgctatat gttccataaa
      241 aacccttatc ttctaaagag tccctgtact tcttaacttc tatatactct tcagccaaaa
      301 cttgtggaac tggttcatct aagaaaaaaa cccctacctc gcctttgcta tttttataaa
      361 agtaatcaat ttctgctata gttcccgatc tataattatc aactggagaa cccaacttat
      421 ggccaaaaat cgctaataat atatcactat tttctacaat ttgtttatta ataacacttt
      481 gcccagagct attatttata taagtaggaa tcgcattcgt ttcccatcga attggcatca
      541 aaattacttt tgtattatct gtattttcta tattccatct atatatggtt tcttctactt
      601 cattcctctt tgctgctata tcacctggtg atgctataaa tacttttatt agatttatgg
      661 ttttcattta ttgtaccttt ctctttatgt ctctttaaag taatttttgt ataaaattta
      721 ttgactttta cactatagga caacttttat ctatttagta caaaaaggcc cactaaacta
      781 attgattagt taggagccta ttacaggtat attttcaact cgcttttaag caaattctac
      841 taccagcttt ttgcctaatg ccctagcgat atcatcaagt ttatcgaaag atacgttatc
      901 ccctctctca attcgagcaa tagtagattg agttgtatgt gctttttgtg caagctccgc
      961 ttgagttagg cctgcttcct cccgtgcatg ataaagagca accgcagttt cgagttttct
     1021 tccttcacgg tcatatgctt ctgcaaaatc tttgtctttt tgcttttctt caatcaattt
     1081 tttaatcatc atttgtgaac cactcctctc taagttcttt tgcatgttta agttcactta
     1141 ttggtgtttt ctccgttttt ttagtaaatc catgggtaat gacatacctt ccattgacca
     1201 ca
//
LOCUS       DACDIP010000192         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDIP010000192 DACDIP010000000
VERSION     DACDIP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.196"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1194
                     /locus_tag="IV257_002900"
     CDS             <1..>1194
                     /locus_tag="IV257_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8815945.1"
                     /translation="GNVTTSNEYSYYRNIPDVKGLTFDVSNQKTLGNLDVCGTIQTLT
                     FYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVYHLSESDHRIEVDVIQDCLPRY
                     IHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETHESLQVSVKDVPLYQEKYSDQQ
                     NVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTFQESDIEKWLKETLNYFAQLFG
                     QLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNWGSYPATNRIWNKDMYYASLPF
                     TMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSNSLSSALLASLYFEHTGDTKFF
                     ERYPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDAFALGKYHTGSNLCLWKACSGL
                     AEIFEA"
BASE COUNT          350 a          215 c          259 g          370 t
ORIGIN      
        1 ggaaatgtga ctacttcaaa cgaatacagc tactatcgga atattcctga tgtaaaggga
       61 ttgaccttcg atgtaagtaa tcaaaagaca ttaggaaacc ttgatgtttg cgggacgatt
      121 caaacgttga ccttttatcg agaaaattta ctaactgagg aaaaaccagg tgtttgggtc
      181 aataaacagc tttctcaaac caattcgtta tccttaaagg ttgaagtaaa cgggattgtc
      241 taccatttgt cagaatcgga tcatcgtatt gaagtcgatg tgattcaaga ctgtttgcct
      301 agatatattc atcattattc gacgtttaag gtcattgtag tatcctttgc tccaatactg
      361 gctaataagc gtttaagtat gcttgttcaa caggtttaca ttgtgaatga aacacacgaa
      421 agccttcaag tttcagtgaa agatgtgcct ttgtaccaag agaagtattc ggatcagcaa
      481 aatgtgttga tttctcaaaa aggaaataag gacacgatcg cacaacagga agtggcaagc
      541 ttcgccattg cttttgtgga cccaaatgct tttgaagaag taatgacttt tcaggaatct
      601 gatatcgaaa aatggctgaa ggaaacattg aactattttg ctcagctatt tgggcagctg
      661 tcaatgccaa acgatagagt tgtccatctc tatcgtcgtg cactttatca aagcttttct
      721 agtttcggta tgaatcgaca gggacaggtc gtcggcagca attggggcag ctatcctgct
      781 actaatcgaa tttggaataa ggatatgtat tacgcatcat tgccattcac catgtttgaa
      841 ccggagttat gtcaaaaaac ggttctatgg tttgatcaat atggcgttaa gtttcctggt
      901 tcaaaattta caggaggtat caatcattct ctgagtaatt cgttatccag tgcgttactg
      961 gctagtcttt attttgaaca tactggggat acaaaattct ttgagcggta tccgcaggtt
     1021 ctactgaatg cttcaagaat tattgaggac atattagctc aacgtcggtc aggggaacca
     1081 atgctttttc catctgtttg gttatcagac gcctttgcac ttggaaagta tcataccggc
     1141 agcaatcttt gtctttggaa ggcctgtagt ggacttgcag aaatttttga agcc
//
LOCUS       DACDIP010000193         1192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDIP010000193 DACDIP010000000
VERSION     DACDIP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.197"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IV257_002901"
     CDS             <1..1076
                     /locus_tag="IV257_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8815946.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DACDIP010000194         1181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDIP010000194 DACDIP010000000
VERSION     DACDIP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.198"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            335..535
                     /locus_tag="IV257_002902"
                     /pseudo
     CDS             335..535
                     /locus_tag="IV257_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          402 a          200 c          222 g          357 t
ORIGIN      
        1 tcagctattc tatattaaca ttgattgcta tggttttagg tacttcattt tataatgctt
       61 ttgtaagtaa gcatgttgag gaatatgaag tattaatgta cttaaaagag aaaaaaacaa
      121 gtaattaata tgagaacacc acgcatagtt tttattttga gatattctag aatgaaacac
      181 attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta agttttttca
      241 tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc ccgttattga
      301 agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa tatttgagaa
      361 acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga tcacctccta
      421 ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt gccagttaga
      481 tatagatacc tttgaaacat ttgaagtgat cctagataaa attaaacaga attgatcaga
      541 aacaagcgaa gccttctctc ggttcgcttg ttttgctatt tattagttgg ctcagggcaa
      601 ctctaagctc gtttgaaaga aaaccgatgg ataatcccct tgtttgtacc actttagaat
      661 acatggatgt acgtattgcc attgactcct attagttact gaaaaaaaat ttaaacaagt
      721 tgagtattgc agataaatag ctccagtgag gtgaataact gctccactca aggcaataga
      781 tagctccatc cagggcaaca aactgctcca ctttcatatt taatagatga ataaccttct
      841 gtataataga aaggcatacc tttcggtatg acaacttata cagaatgtta ttttattggt
      901 tcactatcga aaaattttgg agctttattt ttccggtatt tctcaacgaa cgattagttt
      961 aactgtggga agctccagac atacgattcg agaagttact tctcgagcag acaggaaggg
     1021 ggtgactgaa ctaaccgctg atatgagcga tacttggctg taggaatatc tttttccgga
     1081 aaaacaaaca acagcacgag gttaccttcc tgtagactgg gaatatgttc ataaagaact
     1141 aatgaaaaag aatatgacat tgaaactcct tcatagagaa t
//
LOCUS       DACDIP010000195         1178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDIP010000195 DACDIP010000000
VERSION     DACDIP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.199"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1018)
                     /locus_tag="IV257_002903"
     CDS             complement(<1..1018)
                     /locus_tag="IV257_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8815947.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYS"
BASE COUNT          382 a          236 c          134 g          426 t
ORIGIN      
        1 ctgagtaagg ataatgtgat gttctaaaag aattagcacc tatccatttc attaaattaa
       61 aatctttaat atttacagcc tcattcaaac cacgtccatt aacatacgaa tcctcatgct
      121 taccaaatcc tttaaaataa aaaggtttat tattaatcaa aaatttgcca ttggtaacct
      181 gaacactgcg aataccaaat tcttctgtat aggtatcgat cactttatta tcatcaatta
      241 aactaatttt catactatat aaataagcgt ctagaggttc ccaaaaaatt ggattcttaa
      301 gctgaatcat tcctttcttt ccttcagctt cagctacaag ttgagcctct tcgtcatata
      361 tcgctattaa gatttttccg gtccattctg tatcgatatt gaattctaca cttgcatcca
      421 aatcagttat tcttgtatta atagtaatat cacttatata gtctttcgga gttgtatata
      481 acttaacagg tctatgaata cctgcataat tgaagaaatc aaaattaact aagttttcct
      541 tttttatatc cccattttcc tgtactgttt ccttcataat ccccacaggt aaagtactat
      601 cgtcaataat attgtttaca gcaactgtaa ttcgattatt cccgtctttt acaaaccgat
      661 taatttcaga ctcaaatggt gtaaacccac ctttgtgttg agtaataaac tttccattca
      721 aaaaaacttt tgcttcatgt gttgcagatc caaaacgaag aactaatctt tcttctaata
      781 atcttttagg aagagtcact tctttttcat accacaccca tccaacatga tttctaatgt
      841 tctctgtttc aaataaatca ttataagaag agggaactga aatcggataa ctctcttttt
      901 catttattgg ttctaaagca agttcttcct gtaagtcatc atttgtatct aatttaaatc
      961 tccagagacc gcttaagtca attacttgcc tcgtctctgt cataatggga tacaacatat
     1021 aaaccactcc tatctaaata ttaaataaat gtatccttta aaccacaata agcatctatt
     1081 ggacttactc ccttaaattc agaaagaccc attagtttgt ccaccaatat ttctaaagtt
     1141 tcttgttttc catcataagt attaatataa gtctttac
//
LOCUS       DACDIP010000196         1154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDIP010000196 DACDIP010000000
VERSION     DACDIP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.200"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            400..1032
                     /locus_tag="IV257_002904"
     CDS             400..1032
                     /locus_tag="IV257_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8815948.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          402 a          166 c          177 g          409 t
ORIGIN      
        1 aatgcgcagt tttcgcaaca gattgattta ttttgaaata agactctatc attacaagtt
       61 catccgttgt aagatgggta taggtcattt gtactcactc cttataattc tttggacgga
      121 actattttga gtgtagcaca aatgactttt ttagttgtct agcttaattt tacaatcggc
      181 gttttaaaaa cattctaaat taaatcagta aaaattttta ttctaagatt tttattagaa
      241 ttacgcctaa tttgtgtctc tcagggcaat tctaagaacg tttcaagaga tagcgacact
      301 atcgttatcg aaaaaggcga aaacctatct cattttctgc taatgtaagt tggtttagct
      361 tttgttaata tgactttatg aaaatggagg taattaaata tgatttttcc taaagttaat
      421 gaagcaagct tgaataaagg atcctttttt catatctatg aaaacgaaga ttttgaactg
      481 tataatatcc tttcacaaaa agaggtcatc gaaaaccaaa taatctgcca tccaattatt
      541 tacaaatcta ttttcaagta tgcatttttt gcttccaatg attttcaaag aatggagatt
      601 accgactgta tattcgaaaa ctgtgatttt tcaggctgta tattgacaaa aagcatgttg
      661 catcgcgtga aatttgtcaa ttgtaaatta acaggtacta aattgatgga aagttatatt
      721 gggaacacgt tattcgagaa ttgtaaaatg gactatgtaa atttatctgg ttcaaacata
      781 aaagaaagta cctttgagca ctctgtctta tcaagcgcag attttgtaga ctgttccctc
      841 actaaaataa tgttttctac taatgattta gaaaatgcta gcttttttga tactgattta
      901 aaaaatatcg atttaagtag caactccttt gaaatgattg aagtacgctc ggaacatcta
      961 aaaaattgta cgcttaatca gttgcaagca cttggttttg ctagaaaatt tttacaaata
     1021 aaagtagtat aatcaaaatg aatatataag ataaattttt agcaatacaa atatccataa
     1081 aaaaacattg cctattttaa ctcttcatgt atactgactc tatcttcaaa aactgaatag
     1141 taggagttat tatc
//
LOCUS       DACDIP010000197         1116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDIP010000197 DACDIP010000000
VERSION     DACDIP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.201"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..891)
                     /locus_tag="IV257_002905"
     CDS             complement(<1..891)
                     /locus_tag="IV257_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HAP8815949.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYI"
BASE COUNT          391 a          207 c          171 g          347 t
ORIGIN      
        1 gatataagct tgatgtcgtt cttgaataac acttggttca cctacaatgt agtcaaaaga
       61 aagaaacgct attttctcat gaccataatg aatcaaatgt tccactgcca atgtactagc
      121 ttggtaatca tttgtattta caaataaacg attcatttgt tgttcaagtt ctcttaatgt
      181 ctcttttgaa agatgcgtat caatataaac aacaggacca tcaaaaacgt tatttaattc
      241 caaaaattct tttacacgta caccaacaac aatgaacccc tcaaaacgcc aatttctctg
      301 aatagtttta acatcttctg ctgaattaac attatgcacc aatacaaaat aaccatgatt
      361 ctttgcctgt tcctcaattc cagacaaaac ttctgcggta aatggatcag aaaatacaaa
      421 atcagcatta tcaatagccg aaaaataaag caatcctatt aaaaatgcaa tcgtcttacc
      481 gattccctga cagacagaac acattccact aggatcatta aatgaataag catttgcata
      541 tcctaaacta ggtgttgcaa aacatgaaaa caacacacga aataacgcat tgatgttagt
      601 aatcgtccct aacgttgagc gcgcactgcc accaatcttc ttttgattga ccacaatcgc
      661 cgtagaaaga ttatcaatct catcaacctc tggacgactg tatttaggta aaaataaacg
      721 ggtaaaacta tcataggtgt cattcagttg acgaccagct tcttgggtaa tagtatcaaa
      781 aacaatcgat gattttcctg aaccagatac gctggtaaaa atactaattt tatttttggg
      841 aattttgaga ctgacatttt taagttgttt ctcttgcgtg gataatttca atatactcgt
      901 tcatttggta ctccccttta ttttgaaata gttgagaatg tttcaatgag acacggtatt
      961 tttggcagat gtttagttta cctagaaaga ggtataaaaa tcaagaaaaa aagagaggct
     1021 gactcataag tagttttatt agtcgaaaat gatagtttat aaaaatagga accgcatcaa
     1081 atcaacattc tttagaatgg atttgatgcg tttttc
//
LOCUS       DACDIP010000198         1112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDIP010000198 DACDIP010000000
VERSION     DACDIP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.202"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            67..1107
                     /locus_tag="IV257_002906"
     CDS             67..1107
                     /locus_tag="IV257_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8815950.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          458 a          166 c          188 g          300 t
ORIGIN      
        1 ttgctatttt cgaaaagagt aggtaatttt acataaataa aacaacgtga aagtagagga
       61 tctacaatga ctgattttaa attttttaag gcggatagag tttacaacga attattttat
      121 caatttccaa aagtctttat tgtttctgac gaatacaaaa aaatgaaaga ttcaactaag
      181 attgcctata tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat
      241 gaagaaggta atgtttattt tacttatacg acaaatgaac tatgtagagt attaaactgc
      301 caaaaacaaa aagcgatagc aatcaaaaaa gagttggaat cctttggttt attattacaa
      361 aagcagatgg gatttaacaa acagttaggg aaaaataatc ctaatagact atatctagca
      421 gaattaaaag tctcagaaaa tgatatctac ttactcgaaa aatttgatag agagaatagg
      481 gaaaacgttg ataaatcaga gggtatgaaa atcataccca ccctcgacga aaaatcagac
      541 gctgaatccc ttggggctca agagggtatg aaaatcatac cgtgccaaaa cgttgataaa
      601 tcagagggta tgaaaatcat accagaactt aataataata tattagacac taatagacac
      661 aatatagaca ctgaaaaaga ccgcctacaa gatcaattgt tgttagacaa ttttgagaca
      721 attatgacaa acgacagcat tgctacgttt gtccctgaac gagtattaaa tttgataaaa
      781 acattttctt caagttacag tgaagctcaa aaaaccgtcc agactattca taatgcaaag
      841 aaaaaagctg aaatagaaag tgatatttcg atagtttttg aagaactcga tagttattat
      901 gtcaatgcag aacaagaatt atacacgaca ctgttaaaag cctatcaaaa attaaaaacc
      961 gaaaaagtcg aaaatatcca gaacctgatt tttgtctatg taaaaaattg gtttatcgaa
     1021 aaaccaatag ctgctaaagt atcaagtgaa aaacgtttga attatgcaag ctccccaagc
     1081 actattacga aagactggtt agagtgaaac tt
//
LOCUS       DACDIP010000199         1104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDIP010000199 DACDIP010000000
VERSION     DACDIP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.203"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..330
                     /locus_tag="IV257_002907"
     CDS             <1..330
                     /locus_tag="IV257_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8815951.1"
                     /translation="DFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLTNIENKGQQP
                     SFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQAIY
                     GEKCKFN"
     gene            complement(844..1011)
                     /locus_tag="IV257_002908"
     CDS             complement(844..1011)
                     /locus_tag="IV257_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8815952.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
BASE COUNT          364 a          164 c          188 g          388 t
ORIGIN      
        1 gatttcaaac cattgggtct tgcgattaaa gaagcccgtc ttagtaaagg attgactcgt
       61 gagcaggtag gatcaatgat ccagattgcg ccacgctatt tgacgaacat tgaaaataaa
      121 ggacaacagc caagttttca tgttgtatat gatttagtga ctttgctaaa tatttctttg
      181 gatggattta ttttagggga agaaaacagt cataaaagct ccaaacgtct tcaagtagaa
      241 gcacagttgg attgtctgga tgaagatgaa ttagtgacga ttgggaaagt gattcaagca
      301 atttacggag aaaaatgtaa atttaactag ttcaataata cacaatggat taattaatac
      361 tatcaggaaa gattgattac aaagatgtaa tgaatttttt ctgatttttg ttaatcataa
      421 atagaaaatg aatttatctt tgttacactt aggaactaga gcgaatgata aatagttctt
      481 tggttactct tatcgctgca aagtacagtt cattttttgt tgttgggaaa attttctgga
      541 tttattgcat atcacttcat ccgactaaaa atttttgatt cccatagcga agttcgtttt
      601 tgttccttag gaaaaacttg actgatatac tagttttgaa atgacgagta actctgtact
      661 tcatcaataa tcaagtattg aattttaatt gtatactttt tatgcttgag ggatgtctac
      721 tgtaatacag ctactgcaaa aaaaatcagt acacttattt tcttataaag gatatcaaga
      781 actaagctca atggtatgca tagaaaagaa aaagccgata aaatgagatt ttgatatctc
      841 gaattatcgg ctctgcgtct ttgcgtctgg ctctgtaatt acagttattc taaacttcgt
      901 tatttcaatt aaaatttctt gtctgcattt aggacaaaat agagggaaat tttttaattc
      961 agtatcttcc ctcattttta aacgggtctt attgccacat atagggcaca gtatccacct
     1021 agtatttaca atatctatat cctcctgttc taattaacaa ctcaatattc attttaaaaa
     1081 tttctcttta gttcactatc aatg
//
LOCUS       DACDIP010000200         1092 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDIP010000200 DACDIP010000000
VERSION     DACDIP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.204"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IV257_002909"
     CDS             6..980
                     /locus_tag="IV257_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8815953.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          136 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta ac
//
LOCUS       DACDIP010000201         1067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDIP010000201 DACDIP010000000
VERSION     DACDIP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.205"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..420
                     /locus_tag="IV257_002910"
                     /pseudo
     CDS             50..420
                     /locus_tag="IV257_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            420..>1067
                     /locus_tag="IV257_002911"
     CDS             420..>1067
                     /locus_tag="IV257_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HAP8815954.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          390 a          165 c          219 g          293 t
ORIGIN      
        1 ataagtaata gagctaactc taaaataaaa ctattaggag gagatgtagg tgaaaaagtt
       61 taaaaagaaa atatatttag gattggttgt tggggtgagt atgtttgggt tcatggctca
      121 tactaaacaa gtaactgcag atactaacca aaaagaaagg attatgagcc aattagatga
      181 ctctcaaata agtatgtacc caatgagcta attgtcagtt taaaggctac acaaagaatt
      241 tcaacagaga aaaataaacc gcaaaattta ttgggtgtaa aagttatggt ggaaaaagat
      301 ttgactctta ggacaaatga aagcgattgt aataagcgag atcaagggtt ctgttgcaaa
      361 gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa agacagagag gacagagtaa
      421 tgaatcattt taaaggcaaa caattcaaaa aagacgtcat tattgtcgct gttggttact
      481 acctgcgtta caatctaagc tatcgtgaag ttcaggaatt gttatatgat cgtggaataa
      541 atgtttgtca tactacgatt tatcgttggg tacaagagta cagcaaagtc ctctattatc
      601 tttggaagaa gaaaaataga caatccttct attcatggaa aatggacgaa acctatatca
      661 aaattaaggg acgttggcat tatctttatc gtgcaattga tgcggacggc ttaaccttag
      721 atatctggtt acgaaagaaa cgggatacgc aagcagccta tgctttctta aaacgactcc
      781 ataaacagtt tggtgagccg aaagcaattg tgaccgataa agcaccttct cttggctccg
      841 cctttagaaa gttacagagt gtgggtttat atactaagac agagcaccga actgtgaagt
      901 atcttaacaa tttaatagaa caagaccatc gacctattaa acgacgaaat aaattttatc
      961 aaagtctccg tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata
     1021 aaaagaaccg aagaaatgga acgctcttcg gcttttcggt gtctact
//
LOCUS       DACDIP010000202         1063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDIP010000202 DACDIP010000000
VERSION     DACDIP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.207"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(38..994)
                     /locus_tag="IV257_002912"
     CDS             complement(38..994)
                     /locus_tag="IV257_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815955.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DACDIP010000203         1041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDIP010000203 DACDIP010000000
VERSION     DACDIP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.208"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            40..711
                     /locus_tag="IV257_002913"
     CDS             40..711
                     /locus_tag="IV257_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8815956.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          388 a          161 c          146 g          346 t
ORIGIN      
        1 taaaatatca gttaaaataa aagttaagga gcattgtgta tgacaattaa aactagtctc
       61 caaaatcaag cttaccaatc tattcgccga caaattatct atgctgactt agaacctgga
      121 aaaaaagtat cagaaaaaaa attagaagaa acactttcta ttggtcgtac accaatacgt
      181 gaagcactga ttcaactccg acaacaaggt cttgttgata caattcctca aagcggaaat
      241 tatatctcat taattgattt agattcggcc aaaaatgctc gatttgtcag agaacatttg
      301 gaacgacaaa ttatgttaga gtgttgtgcc aaaatgaata accaaaaaaa acggatttta
      361 gaaactataa tttcagaaca agaaaaagct gttagtcaaa aaaatgctcg tagttttttt
      421 cataatgata atttattcca taaagcttgt tttgaaattg cagagcgaga agaagtatgg
      481 caatggttag aaaataacaa tacacactta gaacgatttc gatggctacg agtaaaaacg
      541 gaaggattaa agtgggaaaa aattatggaa caacattatc aactatttga ggcattaaca
      601 aaacaagatc cagaagaagc taactttcta acatcgttgc atcttcattt aatgcttaat
      661 gagcaaaata ttgttttagc tactcatcca aaatatttca aaaatatata acttttaact
      721 ctttttaaat taaatggttg ttctgatcag aaaaatatcg cttgttttct tattatgtaa
      781 tatatttcat aaactattct gcataacaac tattgtttgt actttttaca ttggataact
      841 aattatgact gctgataact tttgatatta aactagtatc tccaatacaa ataaaaaata
      901 gatatcttgt tcatcaaaat tagttcgtgg atacttacct tcatttacat cattcagcgg
      961 tttcccattt tcttttattc tattatttca ttttctaata gacttggctt catttcgcaa
     1021 tacttttttt caataggcat c
//
LOCUS       DACDIP010000204         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDIP010000204 DACDIP010000000
VERSION     DACDIP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.209"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IV257_002914"
     rRNA            complement(<1..>1035)
                     /locus_tag="IV257_002914"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACDIP010000205         1029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDIP010000205 DACDIP010000000
VERSION     DACDIP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.210"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            147..770
                     /locus_tag="IV257_002915"
     CDS             147..770
                     /locus_tag="IV257_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8815957.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            complement(767..>1029)
                     /locus_tag="IV257_002916"
     CDS             complement(767..>1029)
                     /locus_tag="IV257_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815958.1"
                     /translation="YPFKGINTGSDMAMISGETVEEILANYKEQNIKTLELIKKNIKY
                     YDPLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKELF"
BASE COUNT          370 a          161 c          167 g          331 t
ORIGIN      
        1 tctatcatga aaaattagtt gcttttgtca cttagcgctg aaaatttatg ccatttatct
       61 agaaaaatga cagctttata gtgtataaaa aaattagtgt tgattttgag tgtattttaa
      121 atagtttgag gaagtaggta aaaattttga aaattgctta tattcgagtt tcgtcgattg
      181 atcaaaatga gcaacgccaa attgaagaaa tgaaaaagtt cggcgcagaa aaaattttca
      241 tcgagaaaca atctggagca acgattactc atcgaccgat tttccaggaa gctttagagt
      301 ttgtccgaga ccaagatatt tttattgtcg aagcaatcga ccgacttgga agaaattacg
      361 acgaaatcat tgcttcagtc aattacctaa agaagaaaaa cgtgcagctg atcatcacca
      421 gtctaccgat catggctgaa gcaattggca atcctcattt ggataaattc attaaagatt
      481 taatcatcca aattttggcc atgattgcag agcaagagcg taccgaatct aaacgacgcc
      541 aggcgcaagt cattaaaatt gcaaaagcaa atggcgtcta taaaggtcgt ccaaaactct
      601 atagtgcaga tacaaaaaat cctcaacgac gtttagttta tgaaaacatc gttaaggatt
      661 taaatgaggg catagctatt tcaaaaattg ctaaaaacta caatattact agacaaacta
      721 tttacagaat taagaatgat attgagtata acagtaatca aatgaattaa aaaagttctt
      781 tatacattaa aatttcttct tttaactcat caattctagg agatctaaca tcagaatcag
      841 caattgcatt taataactta tcaatatttt ctttattcgc tagtaaagga tcatagtatt
      901 ttatattctt tttaattaat tccagagttt ttatattttg ttctttgtaa ttagctagaa
      961 tttcttctac agtttcgcct gaaatcatag ccatatctga accagtattg attcctttga
     1021 atggataag
//
LOCUS       DACDIP010000206         1028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDIP010000206 DACDIP010000000
VERSION     DACDIP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.211"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            192..446
                     /locus_tag="IV257_002917"
     CDS             192..446
                     /locus_tag="IV257_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8815959.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="IV257_002918"
     CDS             447..764
                     /locus_tag="IV257_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8815960.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          362 a          162 c          213 g          291 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaa
//
LOCUS       DACDIP010000207         1000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDIP010000207 DACDIP010000000
VERSION     DACDIP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.212"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..673
                     /locus_tag="IV257_002919"
     CDS             <1..673
                     /locus_tag="IV257_002919"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8815961.1"
                     /translation="NSVIGVSNSVKEEFRYKQLIKDKFVLLPNTVNTERIIKMGNEFV
                     EDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVMIGEGPLFDEVKMQIQKRN
                     LTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEAMLLETPVVAYNVGGLKDI
                     IDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISAKKFSNMNRYQGVLEKIYV
                     SAALK"
BASE COUNT          369 a          107 c          177 g          347 t
ORIGIN      
        1 taattcagta attggtgttt cgaattccgt taaggaagaa tttcgttaca aacaattaat
       61 aaaagataaa tttgtgttgt tacctaacac tgtaaatact gaaagaatta taaaaatggg
      121 gaatgaattt gttgaagatg atattgatat tttatatgta ggacgcatgg cagaacctaa
      181 aaatccaata ggtgcaatta atgttttaga aaatgtagta aaaaaatatt caaaacattt
      241 acgtgtcgtg atgattggtg aaggaccatt atttgatgaa gttaagatgc aaatacaaaa
      301 aagaaactta acagatgtta ttgagttaaa gggattcaaa agtaatccct ataagtacat
      361 gaagaaatct aaagttctat taatgccatc tctttgggaa gggtttggcc tagtatcagt
      421 agaggcaatg ttgttagaga cgcctgttgt agcttataac gtaggaggat tgaaggatat
      481 tatcgatgag acaaatggat ttctgtgtgg tagtgaagtt gaattatcta attcactaat
      541 gaagctatta aattcagaag aacttagaaa aaaaatggga gaaaatgctg ctattagtgc
      601 aaagaaattt tctaatatga atcgttatca aggagtatta gaaaaaattt atgtatctgc
      661 agctttaaaa taaaatatga attataaaaa tagagatcaa ttagttttac gaaaaataat
      721 acccattagt attttgtttc aatcaggaat gattttttat tcactgatgc cctcaatagc
      781 aattgcagtt gtttcttttt ttatgttttt gtattatatg tggtgcttaa aaaagtattt
      841 ttgtttagca gatagcttat tgttaacatc aattcttttc attccaacat caactatatc
      901 tatattagga actagttatt ctagcttacc attgacgtgg cataatctaa taataataat
      961 tttgatgtta tatattatta tagaaggaac attaaatcgt
//
LOCUS       DACDIP010000208          983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDIP010000208 DACDIP010000000
VERSION     DACDIP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.214"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..707
                     /locus_tag="IV257_002920"
     CDS             <1..707
                     /locus_tag="IV257_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8815962.1"
                     /translation="KYLPGEQLPTEEELCKIYGVSKITIRKAFKLLAESGTVERLRGK
                     GTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTVLHSKIIKADEFLANKLQINVTDRV
                     YNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQYTSLYKLLEDDYDVKATSSIMEI
                     NGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPFHYSQSTVRCDRISYVIEVTENI
                     TLDTVHKNDSDPKVID"
     gene            complement(805..>983)
                     /locus_tag="IV257_002921"
     CDS             complement(805..>983)
                     /locus_tag="IV257_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8815963.1"
                     /translation="EECLVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYD
                     IKYLRQEVQVKFGI"
BASE COUNT          361 a          147 c          150 g          325 t
ORIGIN      
        1 aaaaatatct tccaggagag caattaccta cagaagaaga attatgtaaa atttatggag
       61 taagtaagat taccattcga aaagctttta aacttttagc agaaagtgga acagttgaac
      121 gattaagggg gaaagggacc tttgtaactc aaaaaaaaga aagtttaaaa ttagctggag
      181 ttcatggatt tagcgattct ctttctaccc gaggacataa aattagatat actgttttac
      241 attctaaaat tataaaggct gatgagtttt tagctaataa attacaaatc aatgtaactg
      301 atagagttta caatattaaa cgtttaatgt gggaagacgg tgccccgatg ggaattgata
      361 attttttcgc atcagaagaa aaatttccaa ctttatttga aaaaggtagc caatatactt
      421 cattatataa acttctagaa gatgactatg atgtcaaagc tacgagctct attatggaaa
      481 taaacggttt atctgcaaca cctgagcttt ccgacttatt gcaatgtata gtaggagatc
      541 cgctatttat tttagaaaag gttggcaaag ataaaaataa tcaaccattt cattattctc
      601 aatccacagt tcgttgtgat agaattagtt atgttattga agtcacagaa aatatcactt
      661 tagatacggt gcataaaaat gattcagatc ctaaagtaat agattgatat taaaagaaat
      721 catacccaaa gcaatcaatt ttgattagat ttgggcatga tttttatttt tactaatatt
      781 ctctaaatac atcaaaaaac ttatttatat tccaaatttc acctgtactt cttgtcgcag
      841 atatttaata tcgtaaataa catcatctgc tagacttaca tcttgagaaa aacgtttatc
      901 tttaattgca agaaccttca tatttgctct tttaccagct tttataccat tataagaatc
      961 ttctatcacc agacattctt caa
//
LOCUS       DACDIP010000209          954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDIP010000209 DACDIP010000000
VERSION     DACDIP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.215"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..578
                     /locus_tag="IV257_002922"
     CDS             <1..578
                     /locus_tag="IV257_002922"
                     /inference="COORDINATES: protein motif:HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8815964.1"
                     /translation="LEDQKGLYHLIKAISMLKNKDNVVVYILGHGTKKELLENLIKAY
                     QLQNVIFLLGGQSNPYKYMSRADLYVMPSITEGFPNALVEAMSTETAVLCADCKSGPR
                     EILSNRALDTICENIEYAESGILVCPMTDSRNYSPQYEECDKNLANAIEELIENDNLR
                     NKYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
     gene            616..>954
                     /locus_tag="IV257_002923"
     CDS             616..>954
                     /locus_tag="IV257_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8815965.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITN"
BASE COUNT          371 a          129 c          169 g          285 t
ORIGIN      
        1 gattagaaga tcaaaaaggt ttatatcatc taattaaagc tatatctatg ttaaagaaca
       61 aagataatgt tgttgtgtac atcttaggac atgggacaaa aaaagaatta ttagaaaatt
      121 taataaaagc atatcaacta caaaacgtaa tatttttgct aggtggccaa tcaaatcctt
      181 ataaatacat gtcacgtgca gatttatatg tcatgccatc tataactgaa ggttttccta
      241 atgcattagt tgaagcaatg tctacagaaa ctgctgtctt atgtgcagat tgcaaatctg
      301 ggccaagaga aatattatcg aatagagcgc tagacacgat ttgtgaaaat atagaatatg
      361 ctgaatcagg catattagta tgtccaatga cagacagtag aaattattcg ccccaatatg
      421 aagaatgtga taaaaattta gcgaatgcta tagaagaatt gattgaaaac gataatttga
      481 ggaataagta tgcaaaagca gcaaaaaaga gagcagaact atatagttat gatgcctttg
      541 aacataactt gttgcaaatt atgaagacat ttaattaatt catatgatga aataaaaata
      601 agggagtata actagatgct gaaaaaaaat acatctttac agaataaaac aatattaata
      661 actggcgcag ccggttttat tggatcaaat ttggttcttg agttattacg tactcagtca
      721 tcaataaaaa tcattggcat tgacaatatg aatgattact atgatgtctc tattaagaac
      781 tatagattat cagagataga aacggaggca aagaaccatc caggatctac ttggaatttt
      841 atcaaaggtt ctattgctga taaagcaaca attgatttcc tctttgataa ttataaacca
      901 agtatcgtgg tgaatttagc agctcaggca ggagtccgtt attcaattac taat
//
LOCUS       DACDIP010000210          898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDIP010000210 DACDIP010000000
VERSION     DACDIP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.216"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..889
                     /locus_tag="IV257_002924"
     CDS             <1..889
                     /locus_tag="IV257_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8815966.1"
                     /translation="CQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIED
                     KMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVL
                     PNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANIN
                     HYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFS
                     LLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFK
                     SFENMRIRIFLINQLINVR"
BASE COUNT          307 a          180 c          167 g          244 t
ORIGIN      
        1 ctgtcaggta tctttgacca ccgttattcg tacattgaaa gagtttaaaa gctatttacc
       61 aaagcaatct aagaagatcc tccccagagt attgatggtt gacgaatttc gttcgcatgc
      121 ttccatagaa gataagatga gctttatttg cgcggatggc gaaacaggaa aattaataga
      181 tgttttgcct acgcgtaaat tacctcgatt aacaagctat ttcttaggtt gtaccaatcc
      241 agaagaagta gaattcttgg tgacagacat gaacgccgcc tacttccagc tcaccaaacg
      301 tgttctgcca aatgcgaaag tcgtgattga tcggtttcat attgtcaaac acatgaatca
      361 agcgttcaat gagttgcgta tccgtgaaat gaatgaactt cgtaaagccg gacagaaaag
      421 ccaggcagaa aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa
      481 ccattatgaa tacaaaacat ggaaaagttt ccgagcacca aaatacccat ttcttactga
      541 agcaatgatg attgatcgat tgcttgagtt ttctccgccc ctaaaggagg cgtatccctt
      601 ttttcatgaa ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt
      661 ggcagaactt cccgaaacgt tagatgacag ctttcgggaa aagcttcaaa accttctgac
      721 ctatgaagaa ggcatcacca acgcaatgat ctatccttat tccaatggaa aaatagaagc
      781 gaagaatacc cacataaaga caatgaaacg agtatcctac ggatttaaat catttgagaa
      841 catgagaatt agaatctttt tgatcaatca attaatcaat gtaagataac aaaaaatc
//
LOCUS       DACDIP010000211          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDIP010000211 DACDIP010000000
VERSION     DACDIP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.217"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IV257_002925"
     CDS             <1..888
                     /locus_tag="IV257_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8815967.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDIP010000212          877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDIP010000212 DACDIP010000000
VERSION     DACDIP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.218"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            27..>877
                     /locus_tag="IV257_002926"
     CDS             27..>877
                     /locus_tag="IV257_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8815968.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNI"
BASE COUNT          307 a          126 c          199 g          245 t
ORIGIN      
        1 tttcaaatat gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg
       61 gtgtgtccat tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg
      121 atgaaaccaa acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc
      181 ccgttgcaaa aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt
      241 tggaagatcc ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca
      301 aagtcaattt gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc
      361 taaaatgtca aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat
      421 cagcaattaa ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg
      481 cagtttatac tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag
      541 gacaccgaaa cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata
      601 aacacgtgtc agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag
      661 gattagaaaa atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg
      721 cagcagattt acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag
      781 gaaatgatcc tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc
      841 cagaagatat ttcgattgtg agttttgata atattga
//
LOCUS       DACDIP010000213          853 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDIP010000213 DACDIP010000000
VERSION     DACDIP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.219"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(463..>853)
                     /locus_tag="IV257_002927"
     CDS             complement(463..>853)
                     /locus_tag="IV257_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8815969.1"
                     /translation="FKQFPLFQGKYMTESEVLDYLLSIDNQLRQSYEVYQELLAAFDA
                     KDFTEFFNLIESLPHSLNDGFKKAICYLKKHTEAITNSLKYPYSNGKLEGKNNLIKVI
                     KRIAFGFRTFRHLRMRVLIQQNICEII"
BASE COUNT          271 a          136 c          165 g          281 t
ORIGIN      
        1 atcagaaaaa cgaacgagag actctcataa cgtgccacta aataaaaatc gtacgttatt
       61 ttaaaaaatg ggttgattct aggcagtact ttgtcggtct atttttagaa aaagaggttc
      121 tttgtcaaac atacatgaat tcgcaagctc attcaccctg aaggatgaaa ggcagaaact
      181 gtagtatact aatggtggaa aggaaaaaaa gccgccagca atttggaaaa gaacgttatg
      241 tcgattgtca aactggcttg ctttatttct cgagaaccct gaaatatagc caatgagcat
      301 gtgtataaaa aattgatttt atatgaaata aagcactcct ttcctaaaat tattggaaag
      361 gagtgcgttt tttatggaaa ttatttaggt tctttgtcaa ttgctattgt tttgctcaaa
      421 tcgtatgtta ggaaatgctt cggcatttcc ttttttgcgt tattaaatga tttcacagat
      481 gttctgttga atcaggacac gcatccggag atgacgaaaa gtccgaaatc caaaagcaat
      541 tcttttgatt actttgatga ggttattctt tccctccaat tttccgtttg aatagggata
      601 ttttaaggag tttgtgattg cttctgtgtg ctttttcaga taacaaattg cctttttaaa
      661 cccatcattc aaggaatgtg gcaatgattc aatgagattg aaaaattcag taaaatcttt
      721 tgcatcaaaa gcagctaata actcttgata gacttcatag ctttgtcgta gttggttatc
      781 tatagagagt aggtaatcaa gcacctccga ctccgtcatg tatttccctt ggaagagggg
      841 aaattgctta aaa
//
LOCUS       DACDIP010000214          853 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDIP010000214 DACDIP010000000
VERSION     DACDIP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.220"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..818
                     /locus_tag="IV257_002928"
     CDS             <1..818
                     /locus_tag="IV257_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP8815970.1"
                     /translation="SNYIFSAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAM
                     KELLFDAKNRGYDETEVNLNIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGY
                     PYWERGETARKNGKVDEAIALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDE
                     GIMRKTRHDVGTLEARRDKAIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRG
                     RQIIQMTDDGTIIKEFDTIASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT          324 a          106 c          185 g          238 t
ORIGIN      
        1 tttcaaatta tattttcagt gcttatagcg attgttataa ttctatgaat gaatatatga
       61 gtgttagtaa ttacactaaa aaagaagcag agcaaagcct tagtatgctg aaatctattt
      121 caaaatcagc gatgaaagag ttattattcg atgcaaaaaa tcgtggatat gatgagactg
      181 aagttaacct aaatattgaa aaagtgatat ctagagaaga tgtaattatt aacgggttgc
      241 ataatcgagt ttcattaaac aagatacgaa atatagggaa ttgggagaaa ggatttgatg
      301 caggttatcc atattgggaa cgtggagaaa ctgctaggaa aaatggcaaa gtagatgaag
      361 ctattgcact ttttgataaa gctcgatata acggctatga tgctcctgcg ctatatgatt
      421 cttatgctaa agcttatcga aaaattaagg attacgataa tgaaattctc attcttgatg
      481 aaggaattat gcgtaaaaca cggcacgatg ttggtacttt agaggctcgt agggataagg
      541 caattaagct attatttgct aaacaagagg cagagcggat agctaaggaa aaatctgatt
      601 ttctaaaagc gaacaaaaaa gaggatgcga ttagtgagcc tagcaagaag cgtggaagac
      661 agattattca aatgactgat gatggaacaa taataaaaga attcgatact atagcttctg
      721 ctgtaaaaga agttggcgtt aactcaaaaa gtattaggga tgctgcaaat ggagttcaaa
      781 aacatgctgg aggatactac tggaaataca aagattagca gataatctaa gataacctag
      841 gatcttctta ata
//
LOCUS       DACDIP010000215          838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDIP010000215 DACDIP010000000
VERSION     DACDIP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.222"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..>838
                     /locus_tag="IV257_002929"
     CDS             6..>838
                     /locus_tag="IV257_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8815971.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          288 a          147 c          159 g          244 t
ORIGIN      
        1 tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag tcttatttca
       61 aaatgaatca atctgttgct aaaactgccc attgcttgaa tcgttcaaga caaacgatcc
      121 ataaagtata tctgttcttc aagcaaggaa aatcagcctt agaatattat caacagtata
      181 agaaaaacaa atcaaactgt ggtagacgtc cgcttgtttt acccgaggaa caatcagaat
      241 atattcaaag aaaggttgtt caaggatgga cacccgatgt gattgttggt cgtgcagcgt
      301 ttcctattag ttgttctgct cgtaccattt atcgtatgtt caaaaagggg ctatttgatt
      361 cttctgactt accgatgaaa ggcaagcgta aaccgaatgg acatcaagaa agacgtggaa
      421 aacaaacttt ccgccgctct attcatgaac gtgaaaagga ttatagccaa ttctcaaatg
      481 agtttggtca ccttgaaggt gacactatcg taggtctgaa acataaaagt gctgtaatta
      541 ccttagttga acgattatca aaagttatca tcacattgaa accgtgtggt agacaagcga
      601 ttgatattga aaaaaaatta aatcaatggt ttgaatctgt accgaaaaac ctattcaaat
      661 ccatcacttt tgattgtgga aaggaatttt caaattggaa acagatcagt aatgtcaatg
      721 atattgccat ttatttcgct gatccaggaa cgccgtctca aagaggccta aacgagaatt
      781 ctaacggatt gttacgtaga gatggtttat tgaaatctat ggatttcaat tcagtaga
//
LOCUS       DACDIP010000216          836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDIP010000216 DACDIP010000000
VERSION     DACDIP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.223"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>836)
                     /locus_tag="IV257_002930"
     rRNA            complement(<1..>836)
                     /locus_tag="IV257_002930"
                     /product="23S ribosomal RNA"
BASE COUNT          183 a          261 c          188 g          204 t
ORIGIN      
        1 tctctcaggg atcttacttt cttaaagaaa tgggaaatct catcttgagg tgggcttcac
       61 acttagatgc tttcagcgtt tatcccttcc ctacatagct acccagcaat gcccttggca
      121 gaacaactgg tacaccagcg gtaagtccat cccggtcctc tcgtactaag gacagctcct
      181 ctcaaatttc caacgcccgc gacggatagg gaccgaactg tctcacgacg ttctgaaccc
      241 agctcgcgtg ccgctttaat gggcgaacag cccaaccctt gggaccgact acagccccag
      301 gatgcgacga gccgacatcg aggtgccaaa cctccccgtc gatgtggact cttgggggag
      361 ataagcctgt tatccccagg gtagctttta tccgttgagc gatggccctt ccatgcggaa
      421 ccaccggatc actaagcccg actttcgtcc ctgctcgact tgttggtctc gcagtcaagc
      481 tcccttctgc ctttacactc ttcgaatgat ttccaaccat tctgagggaa cctttgggcg
      541 cctccgttac cttttaggag gcgaccgccc cagtcaaact gcccatctga cactgtctcc
      601 caccacgatt agtggtgcgg gttagagtgg ccataacgca ggggtagtat cccaccagcg
      661 cctccatcga aactagcgtt ccgatttcta cggctcctac ctatcctgta catgcggtac
      721 agacactcaa tatcaaacta cagtaaagct ccatggggtc tttccgtcct gtcgcgggta
      781 acctgcatct tcacaggtac taaaatttca ccgagtctct cgttgagaca gtgccc
//
LOCUS       DACDIP010000217          831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDIP010000217 DACDIP010000000
VERSION     DACDIP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.224"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..795)
                     /locus_tag="IV257_002931"
     CDS             complement(<1..795)
                     /locus_tag="IV257_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8815972.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIE"
BASE COUNT          300 a          151 c          135 g          245 t
ORIGIN      
        1 ttcaatgaat tttctttgga aaacgatgaa tatcacaact attggccaaa ttgctaataa
       61 cgaacctgcc attaattgtg ggaagttcgt ggcatattgt ccttgtaagt ttgctaatcc
      121 cggagctaaa ggcatcttat caattgaagt attgacaatc aatggccaca ttaaatcttt
      181 aaatgcaaac aacgctgtga atattccaag tgcaaccaat cctgatttag caagcggcaa
      241 cataattttg aaaaagatcg tccaaatatt ggcaccttct aaataggccg cttcctccaa
      301 ttcatcaggt aaacctaaga aaaattgtct taataaaaac gtaccaaacg cactaactac
      361 ccctggtaat attaatgctg gaattgtatt taaaataccc aatttttgtg ctaataaata
      421 ctgtggagta ataaaaatgg aaccgggaat catcatttga ataagtacta agctgaaaag
      481 gatatttttc cctggaaaac ttagacgcgc acaagcatat gcagccattg ctgaaaataa
      541 aaccgagcca atcactcgtc caacaaccat taaaaatgta ttgatataaa aagccatgaa
      601 tggtaataaa tcaagtactt gttgatagtt acttagctga aatttctcag ggaaaattgt
      661 aggtggaatt tgggtagatt caaccaacgt tttccctgaa gttaaaatca tccaaataaa
      721 aggtaatgcc attgcaatac cacctaaaat aagcatgata tggattggcg catttgaaaa
      781 aaatttattt ttcataattt gatagcctcc ttaatagttt acccactttt t
//
LOCUS       DACDIP010000218          796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDIP010000218 DACDIP010000000
VERSION     DACDIP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.225"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..>796
                     /locus_tag="IV257_002932"
     CDS             1..>796
                     /locus_tag="IV257_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP8815973.1"
                     /translation="MEILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGF
                     NSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQK
                     GITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIP
                     LKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVV
                     TDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          271 a          117 c          148 g          260 t
ORIGIN      
        1 atggaaatac taatcaataa aggtgatttg gatgatttgt ttcgtcgtca cctagaattg
       61 gctattaacg ctttattaca agctgaatta acggtatttc ttgactatga aaagtatgat
      121 agagctggat ttaattcagg taattcccgt aatgggaatt attcgcgctc ttttaaaaca
      181 gagtacggtg aattgaattt agtaatacct agagatagga atggagactt ttctcaacaa
      241 actttacctg cttataaaag aaccaatgac tctttagaaa ccactattat tcagctagtt
      301 caaaaaggaa ttactatgtc cgaaatctct gatctaatcg aaaaaatgta tggtcattat
      361 tacaaaccac aaactatttc aaatatgagt aaaattgtgt ctgaagatgt ttcggcattt
      421 aaagaaagaa ctttagaagc taaatactca gtcattttta tggatgctac tcatattccc
      481 ttaaaaagac aaaccgtatc aaaagaagct gtctatatag taattggtat caggttggat
      541 ggaactaaag aggttctagg atttactata gctccaactg aatctgctta tgtttggaaa
      601 gagattcttc aagatctaaa agaccgtggt ttagaagagg ttttgttagt cgtaacagat
      661 ggtttaagtg gtattgacga tagtattcat agtgtttatc caaatgctca atttcaacaa
      721 tgttgtgtcc atatatctag aaatatcgct cataaggttc gtgttagtga tcgacaagaa
      781 atctgtagcg atttca
//
LOCUS       DACDIP010000219          795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDIP010000219 DACDIP010000000
VERSION     DACDIP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.226"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            120..353
                     /locus_tag="IV257_002933"
     CDS             120..353
                     /locus_tag="IV257_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8815974.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            350..757
                     /locus_tag="IV257_002934"
     CDS             350..757
                     /locus_tag="IV257_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8815975.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          296 a          112 c          147 g          240 t
ORIGIN      
        1 taattaatta tatagaaaga acaatctttc tatagagtta gacatgagag ggactatgct
       61 tgatttaaaa tcaacaagcg tatatactgt atatacaaag aaaaaggagg gagaagacta
      121 tggcaattaa agaaagaaaa tttagggaag tggggaactc agtagtatta actatttcaa
      181 aggagtttct taaagaaaat gggttaaatc ccggagatac ggttttttta gatgaagaaa
      241 aacttcgagc ggcaattaca aaaaaacaag ccttatctga tgaaattgat ttagcaatat
      301 ctcaagcatt aactgaatat gatactactt ttagagagct tgtcgataaa tgagtattca
      361 ttacctagaa gccaaagata ttataaaaat gaatgtaatc caaataaaaa aatactctcc
      421 taatgaaccc cttggtgtaa aggatgcgaa tgctttagaa atgtgtgtag ggcaacttcg
      481 agcctcagct tttggtgaag aggtttatcc ttcgatttat gaaaaagcag ctatcctact
      541 tatacaattg ataaagaaac atccttttca caatgcaaat aaacgtactg ctttcttagc
      601 tacttttgtc tttttgaaaa tcaatggaca gcttttagct attgatcaaa aagatgctgt
      661 aaatttagcg gtatatattg ctacgtatga taaggatttc gatcaactaa aatatgaagt
      721 gatcaacact ataaagacac atacgcagcc catttaagga gttactgtat gtgtctttat
      781 agggttatta gctgc
//
LOCUS       DACDIP010000220          777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDIP010000220 DACDIP010000000
VERSION     DACDIP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.229"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..159
                     /locus_tag="IV257_002935"
     CDS             <1..159
                     /locus_tag="IV257_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8815976.1"
                     /translation="NVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGK
                     RIIKKNHR"
     gene            125..475
                     /locus_tag="IV257_002936"
     CDS             125..475
                     /locus_tag="IV257_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815977.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(567..>777)
                     /locus_tag="IV257_002937"
     CDS             complement(567..>777)
                     /locus_tag="IV257_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8815978.1"
                     /translation="VTGGEGWYQEEGKPARRLMAGDVVVTHKGIKHWHGATKDSWFEH
                     LAITAGNSEFLEPVTDEVYEGLENQ"
BASE COUNT          233 a          164 c          138 g          242 t
ORIGIN      
        1 aacgtcagca ctttgcacgg aggactaaat ctatataaca gtggtttttc aactggtttc
       61 gttgcaggta tccttgtacc gatactagat aactttaccg ctgtcagaaa ggaaaaaaag
      121 acacttggaa aaagaattat caaaaagaat catcgatgag cttcttactt atttatattc
      181 ccatgaaata actgaaatac gtattggagt agaatttgct gaggaagggc ttttcataga
      241 gatacagggt acggcagaaa ctcagccaga agatttagat gtacttgctg atttgctgaa
      301 caatcctcgg gaccctagta tggaaatcta ctatcaagaa ctattaggta atccccgaca
      361 tgatcgggaa gattatcatc tgttaggtag tttgattgat gacgtggaaa tcatgtatgc
      421 ccagccgatt atcaatatta agatctttcg acgtgcttct cattcctatt attaacccta
      481 ttttctattt ataaaagact gaatcaaaaa agcagctcct gaaagttcga ttaaaatcta
      541 ctttcaggag ctgttcaagc ttgacattat tgattttcca atccttcata cacttcatct
      601 gttacaggtt ctaaaaactc tgaattacca gcagtgatcg ccaaatgttc aaaccaactg
      661 tcttttgtcg caccatgcca atgtttaatc cctttgtgcg ttacgacgac atctccagcc
      721 attaatcgtc ttgctggctt tccttcttcc tgataccagc cttctcctcc ggtcaca
//
LOCUS       DACDIP010000221          768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDIP010000221 DACDIP010000000
VERSION     DACDIP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.230"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            40..549
                     /locus_tag="IV257_002938"
     CDS             40..549
                     /locus_tag="IV257_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815979.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT          305 a          116 c          135 g          212 t
ORIGIN      
        1 aagtaaggaa ttacaaaaag agaaagaaga gaaggaagaa tgaaaaaggt attatttgcg
       61 gttagtttag ttgttaatat agcactagca ggaggattat tttataccat tacagaaatg
      121 aataagcttg atgaccaacg ggaaagctta gcaatcagta acgaacaata caaaaggaag
      181 ctagatgaag ctagcagcac caacgtggac actcaagaag ccgtagaatc ctctacaatt
      241 gtttatagca atgaatccag tgaaacccta acaaattcgg aaagaagcac agctccagct
      301 acagaaactg tttctcaaaa agctactata acaataggaa gtaaagtatt cacgtttata
      361 gtaaatggct tgacaataga acacgattct agcagaacag gaaaaatgac cgagctttct
      421 catgagtttc cagtgattgt tactactcct agtgctaccc ttaacggtat attggtgcaa
      481 gacaacaatg ataataattg ctatataatg gtagacggtc aaactctata cagaggaaca
      541 taccaataaa ttttgagtct taatatgact gatatttata aaaaattcct gatattagaa
      601 caagaatacg ctgtcttcaa atgtataata ttacaatagc acgatatagc aatattgtta
      661 tatcgtgttt cataataatt aagagcttta aaattagtat ttattagata cttaaacgct
      721 attttagtat tgataaattg ttaccagcaa caaaaaagaa gttactta
//
LOCUS       DACDIP010000222          762 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDIP010000222 DACDIP010000000
VERSION     DACDIP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.231"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            48..>762
                     /locus_tag="IV257_002939"
     CDS             48..>762
                     /locus_tag="IV257_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8815980.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTE"
BASE COUNT          292 a          102 c          125 g          243 t
ORIGIN      
        1 aacataataa aacaaaaatt atccaaatct aacataggag ggtggatatg gaaaattcta
       61 tatatttagt aacagaacct aacactcagc tgaaagattg ttactttaat ttttgtgggt
      121 attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa tacttaatac
      181 acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat tttctgaaga
      241 aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca gatgaaaaaa
      301 atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac tatctaaaag
      361 aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat tcatttaaag
      421 gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa ttgtccagac
      481 aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat ctgaacgaag
      541 ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata atacgtgaga
      601 atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata cacgaaaatt
      661 atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat attagtgaaa
      721 tcaaaatttc tattgcaaac gatctgttat catcaactga at
//
LOCUS       DACDIP010000223          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDIP010000223 DACDIP010000000
VERSION     DACDIP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.232"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..311)
                     /locus_tag="IV257_002940"
     CDS             complement(<1..311)
                     /locus_tag="IV257_002940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815981.1"
                     /translation="MKKIKIMHLLQSNRFSGAENIVCQIMSLFQNNEKFEMIYVSPDG
                     PIANILNIRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSH
                     L"
     gene            complement(334..>742)
                     /locus_tag="IV257_002941"
     CDS             complement(334..>742)
                     /locus_tag="IV257_002941"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8815982.1"
                     /translation="RETLQNIANELGLHQRVFLLGYRSDIPKLLAAANIYILPSLREG
                     LNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEIAGAINRLADNKLLC
                     ESMGAHNLDKIKDFDLSVVINQINNVYHEIVSK"
BASE COUNT          232 a          138 c           90 g          282 t
ORIGIN      
        1 tgcaaatggg aaaggatgat ttgcttctta tatctacttg ctcttacact agcattaaaa
       61 tcatgagcat gaattatatc tgggtttaat tcatgtacgg ctctgtcaat ttcttttctc
      121 gaaaattttt ttaatggtaa gtaacttaaa cctctaatat ttaaaatatt tgctatagga
      181 ccatctgggc taacgtaaat catctcaaat ttttcgttat tttgaaataa actcattatt
      241 tgacaaacaa tattttcagc gccagaaaat ctattacttt gcaacaaatg cataattttt
      301 atttttttca tattaatatc actttcaatt caattattta cttacaattt catggtaaac
      361 attatttatt tggtttatca caactgataa atcaaaatct tttatcttgt ctaaattatg
      421 tgcccccata gattcacaca ataatttgtt atcagccaat ctatttatcg caccagcaat
      481 ttcttcttcg tctttaggat tgattagaaa tccaccctta ccatcttcta taagatctac
      541 atttcctctt atattaccga caatacatgg taaaccacta gccatcgctt ccattaaaga
      601 ggcgttgagg ccttccctta atgagggtag aatatatatg tttgcagcag ctaataattt
      661 gggtatatca cttctatatc ctagtaaaaa aactcgctga tgtaatccta attcattagc
      721 aatattttgt agtgtctccc tc
//
LOCUS       DACDIP010000224          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDIP010000224 DACDIP010000000
VERSION     DACDIP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.233"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..703
                     /locus_tag="IV257_002942"
     CDS             <1..703
                     /locus_tag="IV257_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8815983.1"
                     /translation="RRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQ
                     LIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNY
                     EKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRD
                     ISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNF
                     KLRIMIRHGKTFLTK"
BASE COUNT          235 a          151 c          138 g          217 t
ORIGIN      
        1 tcgacgcttg ccttatttag aacgctattt ttctcggttt tcattagcga ctcgtgaagc
       61 cgttcaattg attgttattg atatgtatgc gccttatgtt tcgttggtga aaaagctttt
      121 cccgaatgct cagttgatca tcgatcgttt ccacatcgtc caacatattg gcagaacttt
      181 cttgaatcat cgagtgaaag aaaccacggt acgacttaaa ggaaattccc actctcaacg
      241 agggttaggg aaaaaattga agcgctattg gaaactccta caaaaagaag aagcaaagtt
      301 aaactatgaa aaacgggttt ggcgtgccag ttttaaagat tatttgactg aaagtgagat
      361 tgccgatcga ctgttagctg cttgtcccaa cttacgccaa ggctatcagt tataccaaga
      421 cattctttat gcggtaaaga aaagagatca gtcactattt gaggattgtt taactagaga
      481 cattagcggt ctgccagaaa cctatacaac cactttacga acgtttaaaa agtttctgcc
      541 acagatccaa aatgcgcttc actacagcta ttcaaacgga ccattggaat gcttaaacaa
      601 ccacatcaaa gtattaaaac gcaatgcgta cggctttcgc aacttttaca atttcaaact
      661 acgaattatg attcgtcatg gcaaaacatt tttaaccaaa taaacagaac agaaagaggt
      721 tttcttcttt ctgttctgtc c
//
LOCUS       DACDIP010000225          740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDIP010000225 DACDIP010000000
VERSION     DACDIP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.234"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            3..>740
                     /locus_tag="IV257_002943"
     CDS             3..>740
                     /locus_tag="IV257_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8815984.1"
                     /translation="MKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGSSR
                     DKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTD
                     GTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRS
                     FGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNS
                     FGKVYSGLIEAGLPSIMAGHIHMPAYSK"
BASE COUNT          250 a           96 c          160 g          234 t
ORIGIN      
        1 ctataaaagt agatttacga aaaaaaccat ataatttaga tgaagatgct atttcttgga
       61 ttgaagagac gattgattct atgactttgg aagaaaaaat aggtcaattg tttgttaata
      121 tgggatctag tcgtgataaa aattacttga gtaatatggt caatcagtac catattggag
      181 ctgtacgata taatccagga actgctgatg aggtatatga tcaaaactat atcttacaaa
      241 cagaaagtaa aattcctcta ttaattgctg ctaatacgga agcaggtggt aacggagctt
      301 gtacagatgg tactgaagtt ggtgtagaag taaaaattgg tgcaacaaat gatgcgaaat
      361 atgcttatga attagggaga gtttctggga ttgaagcatc ggctattgga tgtaactgga
      421 gttttgctcc tattgtagat attaatagaa attggagaaa tcctatcatt tctagtcgat
      481 cttttggaca aaaccctgat aaagttttag aacttagttt agcttatatg aaaggtattc
      541 aggagagtgg aattttgcct gcagctaaac atttccctgg tgacggtatt gacgaacgag
      601 atcagcatct atcttttagt attaatagtt taggtacgga agaatgggat aattcttttg
      661 gaaaagtata ctcaggctta atagaagctg gattaccttc aattatggca ggtcatatcc
      721 atatgccagc ttattcaaaa
//
LOCUS       DACDIP010000226          727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDIP010000226 DACDIP010000000
VERSION     DACDIP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.235"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..723
                     /locus_tag="IV257_002944"
     CDS             <1..723
                     /locus_tag="IV257_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8815985.1"
                     /translation="EDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATLLLTL
                     SWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTVKNDD
                     RITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLLPAGI
                     TSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMCKTIF
                     VIFGKEYHADLEGEREFINQIK"
BASE COUNT          276 a           81 c          130 g          240 t
ORIGIN      
        1 gaagatttgc caaaagaaat gcagaatgat gaaatacggt attattatga tatcctcagt
       61 aaaaaaaaat tcagcttagt ggtaaaaaga gtatttgata ttttagtgtc tgcaactttg
      121 ttactgacac tttcatggtt attttttatt cttgcaatag tgataaaaat tgattctcct
      181 ggacctgtat tttatagaca agaacgtatt actcgttatg gaaaaaaatt taaaatttat
      241 aagtttcgta ctatggtaat aaatgcagat aaagatggca ctcttgttac tgtgaaaaat
      301 gatgatcgta ttacaagagt aggaaaggta atccgtaaat atagattaga tgaaattagt
      361 cagctaatta acgttttggt cggagatatg tcttttgtag gaaccagacc agaagttgga
      421 aaatatgtaa agtcgtattc accattaatg ttagccacat tattattacc agccgggatt
      481 acatcagaaa ctagtatttt atacaaagat gaagataaat tgttgcatga taaaaataac
      541 atcgacaaaa cttatataga aaaaattctt cccagtaaaa tgtattacaa tttgaaaagt
      601 atcagtgaat ttagttgtat gaatgatttg aaaataatgt gtaaaactat ttttgttata
      661 tttggtaaag aatatcacgc cgacttagaa ggtgaacgag agtttattaa tcagataaaa
      721 tgaatgg
//
LOCUS       DACDIP010000227          727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDIP010000227 DACDIP010000000
VERSION     DACDIP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.236"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(28..>727)
                     /locus_tag="IV257_002945"
     CDS             complement(28..>727)
                     /locus_tag="IV257_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8815986.1"
                     /translation="CRHSYDNGESGVEHLVYASSSSVYGTNKKIPYSTEDKVDNQVSL
                     YAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLRAGDNIQIF
                     NYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHIYNIGNNTPENLLDFV
                     DILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSALERDFEFKPSTSLRDG
                     LRKFAQWYKSFYEV"
BASE COUNT          234 a          149 c          117 g          227 t
ORIGIN      
        1 taaactttta taagtaaaaa aatacaatta tacttcataa aaagacttat accactgcgc
       61 aaatttacga agcccgtcac gcaaacttgt acttggttta aattcaaaat ctcgttctaa
      121 cgcactagta tctgcataag taactggcac atctcctggt tgcattggaa ctagttcggt
      181 atgagattca aagtcgtaat cttctggtag cacccctgct gcaattagct cttgttgtag
      241 aatatctaca aaatctagta ggttttccgg cgtattgtta ccaatattat aaatatgata
      301 tggtgggatt ggtagcccat cttccccatc aattttctct ggagcgcact gcataaccct
      361 ctttactcct tcaacaatat catctatata ggtaaagtca cgcttgcaat tcccataatt
      421 gaatatttgg atattatcac cagcacgaag tttatcagta aaaccaaaat aagccatatc
      481 tggacgacca gcaggaccat atactgtaaa gaaacgtagc cctgtagacg gaatattgta
      541 aagtttagaa tatgcatgtg ccattgattc attacttttc ttagttgctg catacaacga
      601 tacttgattg tctactttat cctctgtaga ataaggaatt ttcttattag taccataaac
      661 ggaagacgat gaggcataca ctaaatgttc cacaccagat tctccattat catatgaatg
      721 acgacaa
//
LOCUS       DACDIP010000228          717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDIP010000228 DACDIP010000000
VERSION     DACDIP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.237"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(74..649)
                     /locus_tag="IV257_002946"
     CDS             complement(74..649)
                     /locus_tag="IV257_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8815987.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          240 a          153 c          126 g          198 t
ORIGIN      
        1 cttaatttga caaataataa aatgaaaaaa cagagccaga ttagcattat tttgctaatc
       61 cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgg ggcaacatag
      121 tgatgactaa tgaaacaacg acgatagaca acaagcgatc aaggtagtct gtacccgcct
      181 gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc tgaacgatca
      241 tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc gaagaaacga
      301 tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg tttttatctt
      361 tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt tggattggcg
      421 aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg gacaatcctg
      481 tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg atagaatcga
      541 gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg atcccaatac
      601 acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt ttgttagccc
      661 atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DACDIP010000229          717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDIP010000229 DACDIP010000000
VERSION     DACDIP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.238"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>717
                     /locus_tag="IV257_002947"
     CDS             <1..>717
                     /locus_tag="IV257_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8815988.1"
                     /translation="CQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIED
                     KMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRIL
                     PNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANIN
                     HYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFS
                     LLAELPETLDDGFREKLQNLL"
BASE COUNT          227 a          145 c          140 g          205 t
ORIGIN      
        1 ttgtcaggta tctttgacca ccgttattcg tacgttgaaa gagtttaaaa gctacttacc
       61 aaagcaatct aagaggattc tccccagagt attgatggtt gatgaatttc gttcgcacgc
      121 ttccatagaa gataaaatga gctttatttg cgcagatggc gaaacaggac aattaattga
      181 tgtcttgcct acgcgtaaat tgcctcgatt aacaagctat ttcttaggct gcgctaatcc
      241 tgaagaagta aaatttttag tcacagacat gaacgccgcc tacttccagc ttaccaaacg
      301 tattctgcca aatgcgaaag tagtgattga tcggtttcat attatcaaac acatgaatca
      361 agcctttaat gagttgcgta tccgtgaaat gaatgaactt cgtaaagtag gacagaaaag
      421 tcaggcagaa aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa
      481 ccattatgaa tacaaaacat ggaaaagttt tcgagcacca aaatacccat ttcttacgga
      541 agcgatgatg attgatcgat tgcttggctt ttctacgtcc ctgaaggagg cgtatcccta
      601 ttttcatgaa ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt
      661 ggcagaactt cccgaaacgt tagatgacgg ctttcgggaa aagctccaaa accttct
//
LOCUS       DACDIP010000230          709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDIP010000230 DACDIP010000000
VERSION     DACDIP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.240"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..206
                     /locus_tag="IV257_002948"
     CDS             <1..206
                     /locus_tag="IV257_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815989.1"
                     /translation="RYKIVDSAGIREFINKPDSLFIVVPYEKDGVKKIIFLGSSKDEK
                     RTKEKLIHDGSELNKYKLQKTSE"
     gene            203..445
                     /locus_tag="IV257_002949"
     CDS             203..445
                     /locus_tag="IV257_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815990.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            463..>709
                     /locus_tag="IV257_002950"
     CDS             463..>709
                     /locus_tag="IV257_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815991.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAA"
BASE COUNT          252 a           96 c          151 g          210 t
ORIGIN      
        1 aaagatacaa aatagtagat tctgcaggca ttagggaatt tataaacaaa cctgattcat
       61 tatttattgt tgttccctat gagaaggatg gcgtaaaaaa aataattttt cttggatcta
      121 gcaaggatga gaagcgaacg aaagagaaat taattcatga tggatcagag ttaaataaat
      181 acaaattaca aaagactagt gagtgattgc tagtcttttt atttatcctg aaggaggaat
      241 cacagtggca aaagttaaaa caccattcga aaaagccgcg tctaaaatct tagaacgccg
      301 tggtgtttct gaaatacagt ggaaaaaaga agttcttgaa aaagcacaat ttgacctatt
      361 acgaggaaaa gataaggact tagaagaata tatctatcaa cgtgaacgtg aaaaacttgt
      421 attcgatgaa attattaaac attaaaagga gcgaataatc ttatgtttga atcaattaca
      481 atgaattttt taactcaacc gaaagtagta gctgcagttg atatttttag agcattacaa
      541 actgccgcaa ctagtttgaa aaataatctg atgatttttg ctgctgttgc ggcagtggct
      601 tccattgctc ttggaggtat tttcttcttc tttgggcgaa gaggagctga aaacgggaag
      661 acacacgtag gttatgtatt gttgggggta ggagttattt tagctgccg
//
LOCUS       DACDIP010000231          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDIP010000231 DACDIP010000000
VERSION     DACDIP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.241"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            74..679
                     /locus_tag="IV257_002951"
     CDS             74..679
                     /locus_tag="IV257_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8815992.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          268 a          103 c          128 g          205 t
ORIGIN      
        1 aaatgctatg ataacagtaa aactacaagt aaattttaca tgtaaattat atcatttttt
       61 aaaaggagtt tttatggcaa aaattggata tattagagtg tcttcacaag atcaaaattt
      121 agatcgacaa ttggcaatga tggaagacct taacattgat aaattattcc aagaaaaagc
      181 cagtggtaaa gatatccagc gacctgagtt tcaaaaatta ctaaattata tccgagaagg
      241 tgattgtgtc attgttgcat cactggatcg cctaggcaga gattatgaag acataaaaaa
      301 tacagttacc tttatgaaac agaaaaaagt agcattaaaa atactagatg cacagttttt
      361 agattttaat actggaaatg aactcctaga tactgctatg ttcgatatgt ttttatcatt
      421 gctaagttat attgcacaaa acgaacgtga gaaaatccgt gaacgtcagc ggcaaggagt
      481 attattggcc aaaaaagctg gtcgatataa ggggcgacca attgaatatt ctgagaactc
      541 aacagatccg caaaaacgat tagtctttaa aactgtggtg gacatgttaa atcaaggagt
      601 tccagttgca gaaattgcca aagaaaatgg tctctctcga ccaaccatat ataaaataaa
      661 gaaagcgaat gatttgtaga atttatacta tattcattcg cttt
//
LOCUS       DACDIP010000232          692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDIP010000232 DACDIP010000000
VERSION     DACDIP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.242"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..643
                     /locus_tag="IV257_002952"
     CDS             <1..643
                     /locus_tag="IV257_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8815993.1"
                     /translation="HKTVIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVEL
                     TKELDPQKRPVTIVTYLKSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKE
                     LDGWMERCPDTPIMFTEYGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVG
                     EQVWNFADFATSQGILRVQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          251 a           95 c          147 g          199 t
ORIGIN      
        1 tcataaaacg gtgatcaagg aattgataga acgcgacaag aaccacccaa gtgttgtaat
       61 gtggtctatt gcaaatgaac cagattcgga ttccgaaggt gctaaagaat attttgaacc
      121 tttagttgaa ttaactaaag aacttgatcc gcagaaaaga cctgtaacaa tcgttactta
      181 tctaaaatca acgccagatg tttgtaaggt aggagatatt gtcgatgttt tatgtttaaa
      241 tcgttattat ggttggtatg tttcgggagg ggatctggca catgcaaaat atctgttgaa
      301 aaaagagcta gatggatgga tggaaagatg tcctgatacg ccaataatgt ttacagaata
      361 tggagcagat acagtagctg gatttcatga tacagtacca acaatgttta cagaagagta
      421 tcaagtggct tattacgaag caaatcacga agtaatagat acatgtgaaa attttgttgg
      481 tgaacaagtg tggaattttg cagattttgc aacaagtcaa gggattttaa gagtgcaagg
      541 aaataagaaa ggaatcttta ctagagatag aaaacctaaa atgattgctt actatctaca
      601 gaaacgatgg tcatcaatac ccaatttcag ttataaaaaa taatttggat tttttaagaa
      661 tgttcaaaaa acgattagcc tagcctaatc gt
//
LOCUS       DACDIP010000233          682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDIP010000233 DACDIP010000000
VERSION     DACDIP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.243"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..428)
                     /locus_tag="IV257_002953"
     CDS             complement(<1..428)
                     /locus_tag="IV257_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8815994.1"
                     /translation="MKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSGTTKTD
                     TMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAILLISAK
                     DGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQ"
     gene            complement(444..530)
                     /locus_tag="IV257_002954"
                     /pseudo
     CDS             complement(444..530)
                     /locus_tag="IV257_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
BASE COUNT          227 a          126 c           93 g          236 t
ORIGIN      
        1 tcttgataaa catctggcaa atttattcca ttttgatcaa ttttgttaat aaaaaatatt
       61 ataggtatgt tcatctttct aagtgcatgg aatagtatac gagtttgtga ctgtactcca
      121 tcttttgcag agattagcaa aatagctcca tccaaaacag ataatgaacg gtatacatct
      181 gccaaaaaat ccatgtgtcc aggagtatct acaatattta ctttaacatt ttcccgttga
      241 aaagatgtta ttgcggtctg aatagtaata cctctctggc gttccaaaaa catagtatcc
      301 gttttcgttg taccgctatc tacacttcct aactctttaa ttgctccgct actgtatagt
      361 aagctttctg tcaaagtagt ttttcctgca tcaacatgtg ctaagatacc gatattaata
      421 attttcaatt ttatttcctc cattcaaaag ctcaaaaagc agaaaaaatc ccagtgataa
      481 atactcttat cactgggatt ttaatacaaa aatagctgta tactacacac tgatagccac
      541 agatgcttta tgataaaatg ataaaagtat tcttaaactg ggcacaaaaa accagtcctt
      601 attttaaata aggatgctta attaattatt cccgctatct aattgacagt ttatttaaga
      661 ataccttgcc gcatttttat ta
//
LOCUS       DACDIP010000234          652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDIP010000234 DACDIP010000000
VERSION     DACDIP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.244"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..213
                     /locus_tag="IV257_002955"
     CDS             <1..213
                     /locus_tag="IV257_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HAP8815995.1"
                     /translation="SKNIAVWFFFGAVVMAYLELPLIAIAAVGTIIAIVIAQRDLQLK
                     NISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            210..>652
                     /locus_tag="IV257_002956"
     CDS             210..>652
                     /locus_tag="IV257_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8815996.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLV"
BASE COUNT          173 a          114 c          168 g          197 t
ORIGIN      
        1 agtaaaaata ttgctgtgtg gtttttcttt ggggccgttg ttatggcgta tctcgagtta
       61 ccattgattg caattgccgc agttggaacg attattgcaa ttgttatagc ccaaagagat
      121 ctacaactaa aaaatatatc tgttcatgca gttcaaagcg atgctgctgt aaatgcaacg
      181 cttgaccaag aagaggagga tttctttcaa tgaaattagc tgatggaatt tctaaagatg
      241 agaaaaaatt ggttcggaag atgttttggc gctcgatgac tatgtatgct tcggtgaatc
      301 cggtgacgat gggcggcggt ggtttcgcct attctatgat gcccttcatt aatcatttct
      361 acaaaagtga agaagatcga gctgcggcgc tggaacgtca tacacagtat ttttcgacga
      421 ccattccttg cgcgtccttt gtcatgggga ttgcggcatc gatggagaag gaaaattctg
      481 aaaaagctgg ttttgacgtg gaaagtatca acgcgatcaa aacgagtttg atgggaccat
      541 tggctggaat tggcgattca ttgttctggg gagtttggcg tgtcgttacc gcgggactag
      601 cgatcaactt agccaatgcc ggaaatattt tggcaccaat tatcttttta gt
//
LOCUS       DACDIP010000235          646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDIP010000235 DACDIP010000000
VERSION     DACDIP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.245"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..525
                     /gene="tnpB"
                     /locus_tag="IV257_002957"
     CDS             <1..525
                     /gene="tnpB"
                     /locus_tag="IV257_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8815997.1"
                     /translation="KMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLH
                     EKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQ
                     YKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASIN
                     ILTEGLRLHSMGLA"
BASE COUNT          240 a          103 c          125 g          178 t
ORIGIN      
        1 aaaatggaaa agaaactaaa gcgtgaacaa cgcaaattgt ctaggcgtgc tttattggct
       61 aaaaataaag gtaccaatct atttgaagct aaaaattatc aaaaacaaaa acgcaaagta
      121 gctagattgc atgaaaaagt aatgaatcaa cgtactgatt ttctgaataa gttgagtaca
      181 gaaatgatca aaaatcacga tattatctgt attgaagact taaacacaaa aggtatgttg
      241 cgaaatcata aattagctaa aagtatttct gatgtttctt ggtcaaagtt tgtgactaaa
      301 ttacaataca aggctgactg gtatggacga aaaatcatca aagtagataa atggtttccg
      361 tctagtcaga tttgttcaga gtgcggacat aaagatggca agaaatctct tgaaattcga
      421 gaatggactt gtcctgtttg tcatactcat catgatcgtg atatcaatgc tagtatcaat
      481 attctgaccg aaggtctaag actacattct atgggattgg cttagatcca atcatcaaga
      541 accgtaggaa ctacggggat agcttggtaa ataagagaca ccgctgttag taaagaaata
      601 cgctatcaag tatgctctat tcccaagaag ctcccacttc aagcgt
//
LOCUS       DACDIP010000236          643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDIP010000236 DACDIP010000000
VERSION     DACDIP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.246"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..379)
                     /gene="tnpB"
                     /locus_tag="IV257_002958"
     CDS             complement(26..379)
                     /gene="tnpB"
                     /locus_tag="IV257_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8815998.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(369..563)
                     /locus_tag="IV257_002959"
     CDS             complement(369..563)
                     /locus_tag="IV257_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8815999.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          183 a          137 c          111 g          212 t
ORIGIN      
        1 acattttttg tgttaacccc ccagcttaaa aaactccttt ttttcctggc aaaatagctc
       61 gaggttgatc gatacttaat ccttctaaca accatcgaac ctgttggttc gttaaggttc
      121 gaacttcgtc tttcgtccga ggccatttta acttgccgtt ttctatccgc ttataacaga
      181 gaagaaaacc atcaccgtcc caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa
      241 ataaaaaaac ggcatcttcg taaagctcga gttgatactc ttgttgaata agactggcaa
      301 gaccatcaat tccttttcgc aaatctgttt ttccgcacac aatataaata tgtttggttt
      361 gaagtaaatc ataaagcatt ggacaacagt tctttcaaaa tagcttgaag aatataattg
      421 ttaataccgt tatagatcaa aacttcagaa ccattctctc ttttgatgcg catcgctaac
      481 ttggcagccg cgggtttacc tttttctggt gcactctttt cgtttcctaa ttggacagga
      541 acaatctcat ttaatttata catagaaaac ctccagactt cttgatagct caagtatatc
      601 ttgaggtgca tttgttagta aggtactgtt ctatggggcg ctt
//
LOCUS       DACDIP010000237          641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDIP010000237 DACDIP010000000
VERSION     DACDIP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.247"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            85..>641
                     /locus_tag="IV257_002960"
     CDS             85..>641
                     /locus_tag="IV257_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8816000.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMM"
BASE COUNT          198 a          122 c          121 g          200 t
ORIGIN      
        1 tcttttttgt ataaaaaaat atagaaaaga caccctgaaa tcctttagaa ttaatttggc
       61 taccaattca taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat
      121 attctagacc taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg
      181 caaatttgct tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt
      241 ctttgtgtcg tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg
      301 aatgatgtgt ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag
      361 tcttgccaaa gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt
      421 caaaaagttc gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc
      481 gctcgtcaga aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc
      541 ccattgaatc ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg
      601 gttcgaagtg ttgccagtgc aatgagtttt atcatgatgg a
//
LOCUS       DACDIP010000238          619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDIP010000238 DACDIP010000000
VERSION     DACDIP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.249"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            101..562
                     /locus_tag="IV257_002961"
     CDS             101..562
                     /locus_tag="IV257_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP8816001.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT          272 a           74 c          104 g          169 t
ORIGIN      
        1 ttcagcaaaa cattaaaaaa gcccgtgctg caacacgggc tctttcctca tatatgagct
       61 tctacaaaaa aaatcatatc acagaaatga ggaagaaaaa atggaaaaag agattttaat
      121 aaaatctcca ggaaaaacct tgattaaagt aactgatgat tctatttcaa ttattagaaa
      181 aggatttata aatttagtta atcaaggtat taaaggagaa aaaactatcc cctttaaaaa
      241 tatatcagcc gtacaattaa aaaaaccagg tatgagtaat ggttatatac aatttacctt
      301 gctaggcgga aacgaaagtc gcggaggtat attagcggcc acgaaagatg aaaacaccgt
      361 tatgtttaca aaaaaatatt ggaatgaaat ggaaaattta aaaaaatata tagagaagca
      421 acaatctata ttagataata attccaaaga aacacaaatt tcaagtgcag atgaaattaa
      481 aaagtataaa gagttgttag atgaagggat tattaatcaa gaagaattcg attttaagaa
      541 aaaagaattg cttggtttat aatattcaat atcaaaagag aaagaggatc tattaatgga
      601 cggtatattt ggttttact
//
LOCUS       DACDIP010000239          617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDIP010000239 DACDIP010000000
VERSION     DACDIP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.250"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..614)
                     /locus_tag="IV257_002962"
     CDS             complement(<1..614)
                     /locus_tag="IV257_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8816002.1"
                     /translation="MPFQTSAKITIENLHPKDMSSFFYTINYALVDELEENSLYFHAN
                     WHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLERYWWGEGEFKFYIDGDSEYPTV
                     TSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYPYQSNRDATRDRFETGKLNAVH
                     AFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGNDDVRLFER"
BASE COUNT          177 a          140 c          122 g          178 t
ORIGIN      
        1 tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt gtaaagtgac cgataaatct
       61 tcggtaaaac agatggggtc ttgaagatgc caacgataaa gaccatgtcg tggcattgcg
      121 tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt caaaacgatc tcttgtggca
      181 tctctatttg attggtaagg ataccctaaa aaggctgttg aatagttttt cgcatgtgga
      241 cgtccgaact catcgtactc atgaaaggcc cacgccccgc caaaataatc ttctgaacct
      301 gttgaggtta cggtcggata ctcactatcc ccatcaatgt agaatttaaa ctcgccttcg
      361 ccccaccaat aacgttctag gcaggtaagc gcaaggtaag tacccacgta atagccttga
      421 ccctttaatc gatcaattaa cttataatcc tcctgtaatt tggtaatgcg ttcccgatgc
      481 caatttgcgt gaaaatatag gctattttct tccaattcgt ccaccaaagc gtaatttatc
      541 gtataaaaaa atgaggacat atccttcgga tgtaaattct cgatcgtaat tttagcggat
      601 gtttggaaag gcatttc
//
LOCUS       DACDIP010000240          614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDIP010000240 DACDIP010000000
VERSION     DACDIP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.251"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IV257_002963"
     CDS             <1..390
                     /locus_tag="IV257_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8816003.1"
                     /translation="KALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQ
                     ASDDLIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKP
                     FKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          216 a           91 c          124 g          183 t
ORIGIN      
        1 aaagctttgg ctaaattgaa taaaacagaa ggagtcaaag aagtttaccg acaaatttta
       61 aatgcatttg atgtggggac tttagatagt aatgaagata tctttgtcat taaacgtcgc
      121 tattttacac aagcgagtga tgatttgatt catgtctttt ccaatgagtg gtttgtttct
      181 gagaaagaat tacatgcttc taatctgcaa tatctaccag gtgaagatcc aattcctaat
      241 atgaaagcaa ttatcaacag taaggattac gaaggatata aagccaaaca tccagaagcg
      301 aagccattta aatacccaca agaaatgaag cgagcatggc gtaaaatgtt agatgatgag
      361 ttgattccac ttgagaatga attgaggtaa acgagaagag ttaagaaaat tgtacgattc
      421 aaagatggta gtcaagtgga agtgtagaaa atagggatag atgtctttta tttgttcaca
      481 ttttataacg aagtatttaa gacagctatt gaataatgtt tcctccacct ttttcattca
      541 tttaccccac cccgttgtta tatgaccgtg ttttgagagg aaccaataaa aagcataggc
      601 taactagggg ttcc
//
LOCUS       DACDIP010000241          599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDIP010000241 DACDIP010000000
VERSION     DACDIP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.252"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            79..234
                     /locus_tag="IV257_002964"
                     /pseudo
     CDS             79..234
                     /locus_tag="IV257_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..>599
                     /locus_tag="IV257_002965"
     CDS             253..>599
                     /locus_tag="IV257_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8816004.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNV"
BASE COUNT          248 a           56 c          105 g          190 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgt
//
LOCUS       DACDIP010000242          597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDIP010000242 DACDIP010000000
VERSION     DACDIP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.253"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(164..463)
                     /locus_tag="IV257_002966"
     CDS             complement(164..463)
                     /locus_tag="IV257_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8816005.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          189 a          109 c           72 g          227 t
ORIGIN      
        1 tactagctgt ttttgtgtta attaaaggat aaaatacaat ataaagaaac atctgtttct
       61 ttatttagaa taaaaaaata cagactctgt tcaaataaat gaacctagcc tgcattatga
      121 gattgactat actttttaat tcattgagat gaataactcg tttttataaa tgcccaaaag
      181 taatcggtgc cgaagccatt cctcgaccta attctttttc aaattttcct attgttgata
      241 tctccatata aaacggtgat actggtttag acaattctcc cctgattgct aacggacgta
      301 aactacgttc caaattattt atgactttag gataatactg acctttttct aagcctgtct
      361 ttgcgtctat caaaagtata cgttcctttt ctttgatctc tggatttaaa acgagatcat
      421 ataattttag taccatgttt tctatttctt cacttttaga catcacgatt tcctcccaaa
      481 aatatatttc ttcccactac attctataaa caaagtatac gctacatcta gattttttta
      541 aatcattact ggattgttaa aacctttcaa tacactgtta tggcaacgat ttttatt
//
LOCUS       DACDIP010000243          596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDIP010000243 DACDIP010000000
VERSION     DACDIP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.254"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..>596
                     /locus_tag="IV257_002967"
     CDS             12..>596
                     /locus_tag="IV257_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8816006.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKEL"
BASE COUNT          243 a           50 c          113 g          190 t
ORIGIN      
        1 tggaggtata tatgaaaata ttaactgttg aagatgataa tatgataaga gagggaataa
       61 gtgaatatct ttcagaattt ggatatactg ttattcaagc taaagatgga agagaagcct
      121 tgtcaaaatt taatagcgat ataaatttgg ttatcttaga cattcagata ccttttataa
      181 atggtttaga agtgttgaaa gaaattagaa agaaaagcaa tttaccaatt ctaatcttga
      241 ctgcatttag tgatgaagaa tataaaattg atgcatttac taatttagta gacggatatg
      301 tagaaaagcc attttcattg ccggtcttaa aagctagaat agattcttta attaagaaga
      361 attatggaca tttagagaag tttgaatata aggatctatc ggttaatttt aatagctata
      421 cagctaaaat aaatggtgaa gaaatagatg taaatgcaaa agaactagaa gtattaaaat
      481 gtttattgga taataatgga caagtgttaa caagaatgca aattattgat tatgtatgga
      541 aagatagcga agaaactcct tatgatcgag taatagatgt atatataaaa gaactt
//
LOCUS       DACDIP010000244          583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDIP010000244 DACDIP010000000
VERSION     DACDIP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.255"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>583
                     /locus_tag="IV257_002968"
     CDS             <1..>583
                     /locus_tag="IV257_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8816007.1"
                     /translation="DRNGKFSQQTLPAYKRTNDSLETTIIQLFQKGITMSEISELIEK
                     MYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVIFMDATHIPLKRQTVSKEAVYI
                     VIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRGLKEVLLVVTDGLSGINDSIHS
                     VYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          197 a           94 c          100 g          192 t
ORIGIN      
        1 gatagaaatg gtaagttttc tcaacaaact ttacctgctt ataaaagaac caatgactct
       61 ttagaaacca ctattattca gctatttcaa aaaggaatta ctatgtctga aatctctgaa
      121 ttgattgaaa aaatgtacgg gcatcactat acgccacaaa ccatttcaaa tatgactaaa
      181 atagtttctg aagatattat tgcctttaaa gaaaggtcct tagaatccag atactctgtc
      241 atttttatgg atgctactca cattccttta aaaagacaaa ctgtttcaaa agaagcagtt
      301 tatattgtaa ttggcattcg attagatgga accaaagagg ttcttgggtt tagtcttgct
      361 ccaactgaat ctgcttatgt ttggaaagag attcttcaag acctaaaaga ccgtggttta
      421 aaagaggttt tattagtcgt aactgatggt ttaagtggca ttaacgatag tattcatagt
      481 gtttatccaa atgctcaatt tcaacaatgt tgtgtccata tatctagaaa tatcgctcat
      541 aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt tca
//
LOCUS       DACDIP010000245          576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDIP010000245 DACDIP010000000
VERSION     DACDIP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.257"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>576
                     /locus_tag="IV257_002969"
     rRNA            2..>576
                     /locus_tag="IV257_002969"
                     /product="23S ribosomal RNA"
BASE COUNT          184 a          114 c          166 g          112 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatcta
//
LOCUS       DACDIP010000246          575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDIP010000246 DACDIP010000000
VERSION     DACDIP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.258"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..575
                     /locus_tag="IV257_002970"
                     /pseudo
     CDS             1..575
                     /locus_tag="IV257_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          228 a           98 c          108 g          141 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggcta
//
LOCUS       DACDIP010000247          573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDIP010000247 DACDIP010000000
VERSION     DACDIP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.259"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            73..>573
                     /locus_tag="IV257_002971"
     CDS             73..>573
                     /locus_tag="IV257_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8816008.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVE"
BASE COUNT          210 a           80 c          120 g          163 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gag
//
LOCUS       DACDIP010000248          563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDIP010000248 DACDIP010000000
VERSION     DACDIP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.260"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>563
                     /locus_tag="IV257_002972"
     CDS             <1..>563
                     /locus_tag="IV257_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8816009.1"
                     /translation="CSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELV
                     GIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSI
                     TNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEK
                     LDHSLLGKQRKTVETVFSSLEKLGCQN"
BASE COUNT          196 a          106 c           92 g          169 t
ORIGIN      
        1 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
       61 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      121 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      181 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      241 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      301 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      361 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaatc catgaaaagc
      421 ttgcccttaa gggcattact atatccgttc cgccacgtaa aaacatggat aaatcagaaa
      481 aactagacca cagcttactt ggaaaacaaa gaaaaacagt tgagactgtt ttttcttcct
      541 tagaaaaatt aggttgtcaa aat
//
LOCUS       DACDIP010000249          560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDIP010000249 DACDIP010000000
VERSION     DACDIP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.261"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            194..424
                     /locus_tag="IV257_002973"
     CDS             194..424
                     /locus_tag="IV257_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8816010.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          218 a           84 c           78 g          180 t
ORIGIN      
        1 acaaaaccac tatattctat tatgtatcta acaccctcaa aacagctata aatcaacaat
       61 accgacataa aataaacact atgttcaatc tgagatatat caaaggtaaa cgcatttttg
      121 cccccttttt gcccctcaaa tatcgcttta caacaagaac gtaagttcgt ataatcgttt
      181 tgaggtgatt tttatgatgg aagaatttat tagaaaaaat atcagtgatg aatatgcaga
      241 cttttatgat caaagcagta aaaaagataa attccagatg gatgtttcaa tcttagctat
      301 attagctttt tccgaaaata aacaacctgt aactgcaaaa aaagaaacag tattctctga
      361 aggcaaaata aaaactcgat atatattaga ggtagaaact aattttaaaa acagatcgga
      421 gtaatggtta tgctttttaa tgagacacaa ttatggttta aatttgaccc ttcgaataga
      481 tttatcaaag atttttataa agtatggaat tcagaagttt tctttttagc aatcgaagat
      541 agcttattaa ttaatctcta
//
LOCUS       DACDIP010000250          559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDIP010000250 DACDIP010000000
VERSION     DACDIP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.262"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..277
                     /locus_tag="IV257_002974"
     CDS             <1..277
                     /locus_tag="IV257_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8816011.1"
                     /translation="QYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKY
                     TKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          209 a           83 c           75 g          192 t
ORIGIN      
        1 gcagtatcca cgagtggtta aattgctcat gaatcctgct atattaacct tttataattt
       61 ccctccgtca atcagaagaa ctatttattc aactaatttg attgaaggtt ttaacaaaca
      121 attaaagaaa tataccaaaa gaaaagagca atttcctaat gaagaatctc tggagagatt
      181 tctagtatct caattcaaca actataatca aaagtttcta tgccgagttc ataaaggttt
      241 taaagaaata catgatacat tagaatcaat gttttaactt gatcaacaga atgggctttt
      301 ccatttacac aaaattattg acgctcccga caaaattatg atcttgcgta aagttataaa
      361 atattaataa aaatatttta ttaatgaaaa gcttggtagc acggttattc atatgctaat
      421 ttatttcact ttatagtaag gggagttctt agtatattgt tcaaacttaa atttcatata
      481 atttaattat gtaaattagc tattctaaga acttctacgg ataactgccc gtctaaaatt
      541 taaataagag cttagaatc
//
LOCUS       DACDIP010000251          550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDIP010000251 DACDIP010000000
VERSION     DACDIP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.263"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..349
                     /locus_tag="IV257_002975"
     CDS             <1..349
                     /locus_tag="IV257_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8816012.1"
                     /translation="SHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKR
                     VMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLE
                     GELISRDSVHKIK"
BASE COUNT          199 a           75 c           95 g          181 t
ORIGIN      
        1 atcgcattgg atatataaca actctgaatt tgagcgaatt tatcaagaat tagctgaatt
       61 tactgcaatt gttgttactg gagattattt agcggctaaa ttagtaaaat ttttaaaaaa
      121 taagaataaa cgagtgatgg aacaattatc gattattgga tttgatgata ttacttttgc
      181 agaattaatg gatcctgcgt tgacaacaat acgactaaat ccaggtaaca agggggcgat
      241 tgcttttcac caactgattg gactggccaa acaagaagaa ataaaaaatc aagtactact
      301 attagaggga gaattgattt ctcgagactc tgttcacaaa atcaaataaa aaagctctgt
      361 gaaataagta tagttaacaa taaaatatcc gaacgataga gagaatgaac tgtgatctag
      421 cagttcctcg tcgcaaattc actagaatta gcattattta ctctaaattg ttcggatatt
      481 ttttcttgtt tttaattatg attacagtgc ctatttttaa cgaaatttgc ttattttcaa
      541 aatgtatgat
//
LOCUS       DACDIP010000252          546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDIP010000252 DACDIP010000000
VERSION     DACDIP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.264"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(142..336)
                     /locus_tag="IV257_002976"
     CDS             complement(142..336)
                     /locus_tag="IV257_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8816013.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT          168 a          113 c           84 g          181 t
ORIGIN      
        1 aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga
       61 tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca
      121 tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat cgacgaacgt
      181 cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg ccgctttgtc
      241 tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat tgtctctgct
      301 tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc ttctctttct
      361 tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa caattcattg
      421 aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat tcacttaaca
      481 actaacactc tgtgagtgaa atggactctt caatgtgatg ttaggatgat ttcatggaca
      541 cgattt
//
LOCUS       DACDIP010000253          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDIP010000253 DACDIP010000000
VERSION     DACDIP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.267"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IV257_002977"
     tRNA            complement(5..90)
                     /locus_tag="IV257_002977"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IV257_002978"
     tRNA            complement(107..178)
                     /locus_tag="IV257_002978"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IV257_002979"
     tRNA            complement(190..262)
                     /locus_tag="IV257_002979"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IV257_002980"
     tRNA            complement(280..361)
                     /locus_tag="IV257_002980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IV257_002981"
     tRNA            complement(369..441)
                     /locus_tag="IV257_002981"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IV257_002982"
     tRNA            complement(445..517)
                     /locus_tag="IV257_002982"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDIP010000254          522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDIP010000254 DACDIP010000000
VERSION     DACDIP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.268"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IV257_002983"
     CDS             <1..390
                     /locus_tag="IV257_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8816014.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            443..522
                     /locus_tag="IV257_002984"
                     /pseudo
     CDS             443..522
                     /locus_tag="IV257_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
BASE COUNT          195 a           94 c           87 g          146 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc ccaatggaac accagaaaat catttttcaa accagatcag
      481 cttgaatgtt tatagtcgtg ggatgggagg attgggctta cc
//
LOCUS       DACDIP010000255          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDIP010000255 DACDIP010000000
VERSION     DACDIP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.271"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV257_002985"
     CDS             complement(<1..132)
                     /locus_tag="IV257_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8816015.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACDIP010000256          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDIP010000256 DACDIP010000000
VERSION     DACDIP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.272"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..434)
                     /locus_tag="IV257_002986"
     CDS             complement(<1..434)
                     /locus_tag="IV257_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8816016.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
BASE COUNT          146 a           85 c           80 g          199 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc
//
LOCUS       DACDIP010000257          507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDIP010000257 DACDIP010000000
VERSION     DACDIP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.273"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>507
                     /locus_tag="IV257_002987"
     CDS             <1..>507
                     /locus_tag="IV257_002987"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8816017.1"
                     /translation="DKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPF
                     VGIYGLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELA
                     YGRSFVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNYV
                     MQLLSVCL"
BASE COUNT          152 a           60 c           98 g          197 t
ORIGIN      
        1 atgataagac atatttagat tttattaaaa ttgtttccat ttttattatt cctctgggta
       61 taggtatata tgtatatagg gaactagtaa caaatattct actcgggagt caatggaaag
      121 aggctgtacc atttgttggg atatatggat taatgagctg ttttactctt gtcttgggac
      181 aatatgcctc tgagtactat cgtggtttag gaaaaccaaa agcaaatgtt cttatgacta
      241 tcctacattt agcagtatta attccagttc taataatcta ttcaaggaaa gggtttatag
      301 aactagcata tggtagatct ttcgttaaga tagagcagat tatagtcttt tggattattt
      361 tatggtttgg ttttaagttt aatccattta aaattttaaa gcaggtttgg aaatcttgtg
      421 ctagtgcatt gctaatggga gttctagcaa aaattatgtt ggaattctgt actaattatg
      481 ttatgcaatt actttcagtg tgtcttt
//
LOCUS       DACDIP010000258          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDIP010000258 DACDIP010000000
VERSION     DACDIP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.274"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>479
                     /locus_tag="IV257_002988"
     CDS             <1..>479
                     /locus_tag="IV257_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8816018.1"
                     /translation="KAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNP
                     EGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSS
                     DEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPTLPEGTTVTDVTP"
BASE COUNT          199 a           75 c          106 g           99 t
ORIGIN      
        1 aaagcaaaag atggttatac agatgcgaaa tggccagaag aagcaactca accaattaca
       61 gcagatgata cagaatttgt atcaagtgca acaaaactgg atgataaatc tgacgctgac
      121 aaatataatc ctgaaggtca aaaagtgact acagaattga ataaggaacc agacgcatct
      181 gagggaatta agaacaaaga agatttacca aaagatacta agtatacttg gaaagaaaaa
      241 gtagatgtta gcgcagctgg aaataaaaaa gggactgttg tagtgacata tccagatgga
      301 tcatctgatg aagttgaagt agatgttaca gtgacagaca atcgttctga cgccgataaa
      361 tatgagccaa cagtagaagg cgaaaaagta gaagttggag gcacagtaga tctaacagat
      421 aatgttacta acttgccaac attaccagaa ggaacaacag taacagatgt cactcctga
//
LOCUS       DACDIP010000259          478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDIP010000259 DACDIP010000000
VERSION     DACDIP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.275"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..335
                     /locus_tag="IV257_002989"
     CDS             <1..335
                     /locus_tag="IV257_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAP8816019.1"
                     /translation="INDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGLHDQLEE
                     KNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMIGKRLLL
                     ESEKVVKE"
BASE COUNT          175 a           62 c          101 g          140 t
ORIGIN      
        1 atatcaatga tatagacata cattgtacac atgcggactt ggatcttaag gctttacaaa
       61 ttgattatga ttatttccta ccagatgaat atgctagaaa tctctatcat ggactgcatg
      121 atcaattaga agaaaaaaac gaagaaaata tgttttataa aatcgttcaa tctcttgaga
      181 atgagacggc taaaaaattg ttaattcaat tttacgaaga acaagcagca gatcgacttc
      241 aagaatatgt tgaaaagttg atcaaaaata aagaaatttc tgaaatgatt gggaaacggt
      301 tgttattgga atctgaaaag gtggtgaaag aatgatcatt ggaggaattt tttctggcat
      361 agccgactgg tggtcaggct tattcggaga aggggatgca aaccccaaaa ccgtaacgaa
      421 agtcttacaa gattatggga attatctgca gtatcaagat gtatttagtt ggatttgg
//
LOCUS       DACDIP010000260          449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDIP010000260 DACDIP010000000
VERSION     DACDIP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.277"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>449
                     /locus_tag="IV257_002990"
     CDS             <1..>449
                     /locus_tag="IV257_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8816020.1"
                     /translation="YRFDFRKVKNLDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGL
                     NFPGNADYKELQEGKDNVAPFEFIPWILGQCSTIDEAKELLATINLVNIDFSEKLPLS
                     PLHWLLADKEKSIVIESMKDGLHLYDNPVGVLTNNPPFDYQLFNLNN"
BASE COUNT          162 a           66 c           83 g          138 t
ORIGIN      
        1 tatcgtttcg attttagaaa ggtaaaaaat ttagataaac attatgcaat gataggaata
       61 gcagctgggg tatccaacta tccactctat tatgaggcta caaatgaaaa agggttgagt
      121 atggcaggac tgaattttcc tggcaatgct gattataaag aattacaaga aggaaaagat
      181 aacgttgcac catttgagtt tattccatgg atactagggc aatgctcaac catagacgaa
      241 gctaaagaat tattggctac tatcaatcta gtcaatatcg attttagtga aaaactaccc
      301 ttgtcacctt tacactggtt attagctgat aaagaaaaat cgatagtaat tgaaagtatg
      361 aaagatggac ttcatttata tgataatcct gtcggtgtgt tgacaaataa tccgccattt
      421 gattatcaat tatttaatct gaacaatta
//
LOCUS       DACDIP010000261          443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDIP010000261 DACDIP010000000
VERSION     DACDIP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.279"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..344
                     /locus_tag="IV257_002991"
     CDS             15..344
                     /locus_tag="IV257_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8816021.1"
                     /translation="MNAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLN
                     QQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQI
                     VNEHTIQ"
BASE COUNT          213 a           53 c           61 g          116 t
ORIGIN      
        1 gaaaacaaaa tgaaattaat gcgaaaatag acgaaattaa ccaaaaagta gctgaaataa
       61 atcgattaaa tagcgattgg aataatcgtt tacaaaatga aaagaacaaa tttaatgaag
      121 aacaatctac tattaatagt ttaaaccagc aaattaatca aatgttgagt gaaaaaaata
      181 acattcaaaa tcaactgcaa gctactcagc aaaagctaga agaagaaaag tcaaaaaata
      241 gtagtttgaa taattacatt gaaaagcttc aaaaagctaa aaatgatgta gaggataccg
      301 caaataaagc acaacaaatt gtaaatgaac atacaatcca atagatatag ttttaattta
      361 acaaatggta tgtttggaga taatgagata gaatataaat gtcataatat tatacaatct
      421 aaacaccgtt tccttttcta ttt
//
LOCUS       DACDIP010000262          440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDIP010000262 DACDIP010000000
VERSION     DACDIP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.280"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..>440
                     /locus_tag="IV257_002992"
     CDS             11..>440
                     /locus_tag="IV257_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8816022.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DACDIP010000263          437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDIP010000263 DACDIP010000000
VERSION     DACDIP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.281"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..>437
                     /locus_tag="IV257_002993"
     CDS             46..>437
                     /locus_tag="IV257_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8816023.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTP"
BASE COUNT          164 a           79 c           72 g          122 t
ORIGIN      
        1 gaataaagtt aacgacaacc aattcacaga aaagaggact tccctatgaa tgattttact
       61 acagaaattg tgcaaactct agtcactaaa ggcgatttaa atgaattatt ccgttcgcac
      121 ttagaaaaag cgataaacac actcctacgg actgaattaa cggctttttt agattacgaa
      181 aaatatgatc gcactggttt taattcaggt aattcgagaa acggttctta ctttcgatca
      241 atcaaaaccg aatatggtga attaacattg gaaataccta gagatcgtaa tggtgagttt
      301 aaacaacaaa ctttaccagc ctacaaaaga acaaacgata cattggaaac cactattatc
      361 catttattcg aaaaaggtgt tacgatgtct gaaattgctg atttgatcga aaaaatgtac
      421 ggtcatcact atactcc
//
LOCUS       DACDIP010000264          428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDIP010000264 DACDIP010000000
VERSION     DACDIP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.284"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>428
                     /locus_tag="IV257_002994"
     CDS             82..>428
                     /locus_tag="IV257_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8816024.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHV"
BASE COUNT          150 a           78 c           84 g          116 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgt
//
LOCUS       DACDIP010000265          428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDIP010000265 DACDIP010000000
VERSION     DACDIP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.285"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>428
                     /locus_tag="IV257_002995"
     CDS             82..>428
                     /locus_tag="IV257_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8816025.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHV"
BASE COUNT          148 a           74 c           86 g          120 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgt
//
LOCUS       DACDIP010000266          401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDIP010000266 DACDIP010000000
VERSION     DACDIP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.287"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>401
                     /locus_tag="IV257_002996"
     rRNA            <1..>401
                     /locus_tag="IV257_002996"
                     /product="23S ribosomal RNA"
BASE COUNT          125 a           87 c          117 g           72 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt c
//
LOCUS       DACDIP010000267          362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDIP010000267 DACDIP010000000
VERSION     DACDIP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.291"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>362
                     /locus_tag="IV257_002997"
     CDS             <1..>362
                     /locus_tag="IV257_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8816026.1"
                     /translation="DIQKMTLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVP
                     NVKDAVKVAKAFPTEIELMNVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELY
                     PDTVFQPTPAMEKRSAIS"
BASE COUNT          125 a           65 c           81 g           91 t
ORIGIN      
        1 gatattcaaa agatgacatt gaagatggct gcaccatcga atgtcaaagt cattattaaa
       61 acagttgatg acgcgattca tttgttaaaa gatccgcggg cgaaggacat gcgcttgatg
      121 gttttagtcc ctaatgtcaa agatgcggta aaagttgcga aagctttccc aacggaaatc
      181 gaattaatga acgtggggaa tgctgggaaa atgacaccgc catcggaaga taagaaaacc
      241 ttatcaaagg aagtgatgtt aacgacgtca gaaattcaag acttaaaaga attagttgaa
      301 ctttatccag ataccgtttt tcagcctaca ccggcgatgg aaaaacgttc agctatctct
      361 at
//
LOCUS       DACDIP010000268          349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDIP010000268 DACDIP010000000
VERSION     DACDIP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.denovo.294"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(44..>349)
                     /locus_tag="IV257_002998"
     CDS             complement(44..>349)
                     /locus_tag="IV257_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8816027.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           98 a           86 c           64 g          101 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggt
//
LOCUS       DACDIP010000269         6033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.guided.101, whole genome shotgun sequence.
ACCESSION   DACDIP010000269 DACDIP010000000
VERSION     DACDIP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.guided.101"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..221
                     /locus_tag="IV257_002999"
     CDS             <1..221
                     /locus_tag="IV257_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8816028.1"
                     /translation="SMDDRRQILCKLRKRNILMYRQLERLKHDLLRLESKRVQCELEG
                     NQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            234..2312
                     /locus_tag="IV257_003000"
     CDS             234..2312
                     /locus_tag="IV257_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8816029.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2472..4358
                     /locus_tag="IV257_003001"
     CDS             2472..4358
                     /locus_tag="IV257_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8816030.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4370..5317
                     /locus_tag="IV257_003002"
     CDS             4370..5317
                     /locus_tag="IV257_003002"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8816031.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5337..5864
                     /locus_tag="IV257_003003"
     CDS             5337..5864
                     /locus_tag="IV257_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8816032.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(5927..>6033)
                     /locus_tag="IV257_003004"
     CDS             complement(5927..>6033)
                     /locus_tag="IV257_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8816033.1"
                     /translation="FFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         2003 a         1000 c         1316 g         1714 t
ORIGIN      
        1 tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt
       61 atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt
      121 gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa
      181 aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat
      241 ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca
      301 ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga
      361 cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac
      421 cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga
      481 caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt
      541 tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag
      601 gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg
      661 cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc
      721 agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc
      781 tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa
      841 aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg
      901 aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca
      961 ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg
     1021 atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg
     1081 cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag
     1141 acggtttata tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt
     1201 ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt
     1261 tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca
     1321 gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt
     1381 ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg
     1441 tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga
     1501 ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa
     1561 aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt
     1621 acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt
     1681 tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg
     1741 gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt
     1801 tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc
     1861 cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata
     1921 tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata
     1981 acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg
     2041 aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc
     2101 tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg
     2161 aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt
     2221 tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag
     2281 caaagtcaaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa gttttataaa
     2341 aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa
     2401 gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat
     2461 aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa
     2521 aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg
     2581 ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg
     2641 acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag
     2701 cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct
     2761 tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag
     2821 accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt
     2881 ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata
     2941 aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga
     3001 ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga
     3061 tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg
     3121 atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat
     3181 acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag
     3241 aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg
     3301 ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa
     3361 aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac
     3421 gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac
     3481 tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa
     3541 atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg
     3601 gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa
     3661 tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag
     3721 cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta
     3781 tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga
     3841 aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca
     3901 tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg
     3961 tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa
     4021 acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga
     4081 cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga
     4141 agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac
     4201 ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc
     4261 gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg
     4321 cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc
     4381 gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg
     4441 cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc
     4501 tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt
     4561 aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg
     4621 ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg
     4681 ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt
     4741 ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat
     4801 ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa
     4861 acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg
     4921 gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta
     4981 tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg
     5041 cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg
     5101 tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat
     5161 cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa
     5221 tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga
     5281 cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt
     5341 tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt
     5401 ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga
     5461 tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg
     5521 ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag
     5581 acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg
     5641 tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat
     5701 tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta
     5761 gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta
     5821 atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga
     5881 ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata
     5941 aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca
     6001 tatagcggat agaattcaag ctataaaaga atg
//
LOCUS       DACDIP010000270         5050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.guided.113, whole genome shotgun sequence.
ACCESSION   DACDIP010000270 DACDIP010000000
VERSION     DACDIP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.guided.113"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..289
                     /locus_tag="IV257_003005"
     CDS             <1..289
                     /locus_tag="IV257_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8816034.1"
                     /translation="QGVTLRTFESMYFQKKLVTDNQAIAHYQFYHSENIFLLQERDLD
                     ELPEFLHTPFQPIPSELLEFFDAENWAKRFFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(432..1373)
                     /locus_tag="IV257_003006"
     CDS             complement(432..1373)
                     /locus_tag="IV257_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8816035.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1499..2620
                     /locus_tag="IV257_003007"
     CDS             1499..2620
                     /locus_tag="IV257_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8816036.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            2878..4380
                     /gene="lsa"
                     /locus_tag="IV257_003008"
     CDS             2878..4380
                     /gene="lsa"
                     /locus_tag="IV257_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8816037.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            4447..4974
                     /locus_tag="IV257_003009"
     CDS             4447..4974
                     /locus_tag="IV257_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8816038.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
BASE COUNT         1635 a          910 c         1076 g         1429 t
ORIGIN      
        1 gcaaggagtg actttgcgaa cgtttgaatc tatgtatttt caaaagaaac tagtgacaga
       61 taatcaagcc atagctcatt atcagtttta tcattctgaa aatatttttt tgcttcaaga
      121 acgtgattta gatgaattac cggaattttt gcatacgcca tttcaaccaa tcccatcaga
      181 actgttggaa ttttttgatg cagaaaattg ggcaaaacgt tttttcacta ctgactccgc
      241 tatttatgaa caatatgaat acgatctttc ctctgttaaa aataaataaa agtgccaacc
      301 aatcatagat gaaaataaac aagcgcaatt cttatggcaa catgagaatt gtgtttttct
      361 ttttgaactt tcatgggggt aaaaaaatga gaaccagctg ctgatccagc tgattctcat
      421 cagagagcct tttattctgc gccaaggatt ccttcttttg catggactaa ttgggtcaaa
      481 aaggcacaat atggtgtagc aatatcatat ttttgtcctt ttcgccaaac ggcaccattg
      541 atgtagtcga tttcagtcaa acgatggttt ttgatcaggt cttggtacat agatgggtaa
      601 tgcaacccga tgttgtcagg atcataagta gaagcaatat gttggtacac ttcttcttga
      661 tctaatgtga ttccttcttt tgctgcgata gaagcaaact cagatacaat cgttcgaacc
      721 atgccctctg ccgctttttg cgcacctaat tcagcaatat tacaatccaa tatcgtgcat
      781 aatccattta atgttccgtt tacacaagct tttcgccaaa tggaataacg aacattatga
      841 ctatagattg gattcaattt tgcttcttga aagacttcta caactttttt tgtaaatgct
      901 tcgccttctg gttccaagtt ctctagttcg atttctccat cacctaataa tgtgactttt
      961 ccgggaccag ctagaccagc tgtccacatc gtgatcccaa ataagatatt tttctttggt
     1021 acatattttt ccagtacatc ttcatgacct aatccattca gtaaacacaa aacataagta
     1081 ttctcagtaa taatggattg gatcgaacga aacatatcat ctaattgatt cgcttttgtc
     1141 aaggctacga tcaaatccac gtgtttgttg ctttcgatga tttcttctgg tgaatagatt
     1201 ggcagttttg caactacttc tttgccatta aagtcagcaa tcaatccatt tgtacgaatg
     1261 gcttcaatgt gagcaggcca gcgatcgatc aagacgacct catttccgct ttgatgcaac
     1321 attaatccga ttcggctgcc cattgctccg gctccagcta ttgcgatttt catgtttttt
     1381 ctctccttta ttcaatgagt tcccttttag tatatagaaa aacagaagaa aatagcattg
     1441 ttttgtttga aaaaagtgaa aaaaagtgtc ttacttaaga agaaagtgag ggatatagat
     1501 ggatattttg gcggcaatcg attcatttaa aggaagtgct tcttcatttg aattaaatca
     1561 ggcagttcta aatggttggg aagaccgtaa aggaagaaaa atcaatgtgc cgattgcaga
     1621 tggcggagaa aatactgcag aagcaatcta ttacgcgctc gggggaacct ggcgtacgat
     1681 tgatgggatg gatctcttat ttcgtcagcg aaaaatcagt tatttattga cgaactggca
     1741 aggaaaaaat accgcagtca ttgaatctgc acaagtatta ggattagatt tacttgataa
     1801 accaacggat caaaccatcc gacttgcttc tagttatgcc ttgggagagc ttatcaaaga
     1861 tacactgctt caagaagtgg aacaaatcat tgtcacttta ggaggaagcg gatgtagcga
     1921 tggtggtcta ggattactcc aatctttggg tgcttcttta gaagggtaca cagaaggaaa
     1981 tcctttgtta tctactagaa aacttcattt ggatcgaagg aacgttcttg tttctaaagt
     2041 aaaagtgatc atcgctgcag acgtgaccaa cccatataca ggtcaacaag gagctgcttg
     2101 gcaatttgga agacaaaaag gaggaacgga tacgacgtta gctttcttgg ataaacaagc
     2161 tgaaaagatt gctcagcaga taaaagatgt ttgggaggtg gatttagatg acatcccagg
     2221 aagcggagca gcaggcggta ttggcggagc cctttttttg ttaggagcga agatggtctc
     2281 tggttttgag ttggtcagcc aaatcactgg attagaaaaa cagatcgaac aagcggatgt
     2341 catcattaca ggagaagggc ggattgatca gcaaacaatc cacggaaaag ttccatatgg
     2401 cgtagcagtg cttgcaaaaa aatatcgaaa accaataatc gctatttgcg gaagtagaga
     2461 ccgagagctg gaaggactct ttgattttct ccccattgtc ttaagcattc aattaggacc
     2521 aatcgattta gaggaagcaa tggaacacag gcaaacatta gaaaaaacag aaatcttagg
     2581 acagacactc agcagatttt tcaacattct cccgaagtag ttgacaaaaa caatttcttt
     2641 atgctatcgt aatttcaaat ataggacaaa gtgagggatc cgtgcattga aaaattaagt
     2701 cgattgatga aagctgcatt cagactgtac cttgtggaga caagggagca gtgtgcagat
     2761 tttcgggatt ggcttggttc tttcgatgta tgggtctttt tgtgtgcccc aacccttgca
     2821 tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga tggaataatg
     2881 tctaaaatcg aaataaaaaa tctgacattc ggctatgaca gccaaggcac attattattt
     2941 gaacaagcca atctaaactt tgacacacaa tggaaactag gacttatcgg acgaaacggt
     3001 cgaggaaaga caactttact gaatatcctg caaaacaaac tcccttatca agggcaagtg
     3061 atccatcagc aagcgtttgt ctatttcccg cagcaaacaa aagataaaga gcgtttgacc
     3121 tattatgtgt taaatgagat tatggatttt gagatatggg aaattgaaag agagctccaa
     3181 ctgatgcaaa cagatcctga aatcttatgg agagaattca gcacactatc aggaggagag
     3241 aagacaaaag tcctactggc actcttattt gtggatgaca ctcatttccc gttaatcgat
     3301 gaaccaacga atcatttgga tatctctggc agaaaacaag tagcggctta cttgaaaaag
     3361 aaaaaacaag gattcatcgt ggtgagtcat gatcggggat ttatcgatga agtagtggac
     3421 catgttttag caatcgaaaa gagccaactg gaactttatc aagggaattt ctctatctat
     3481 gaagaacaga aaaaacttcg tgatgaattt gaaatagcta aaaatgaaaa attgaaaaaa
     3541 gaagtcagca ggctaaagaa aacagcagct gaaaaagcag aatggtctcg ttcccgagaa
     3601 ggggacaaaa caaagaaaaa agtcggattc atcgatacgg aatctaggcg agtgaataaa
     3661 ggagcagtag gcgctgatgc tgcacggacg atgaaacgat ctaaagcaat cgtgaatcgg
     3721 atggaaaccc agatcagtga gaaagaaaaa ttattaaaag atatcgagta tatcgatgct
     3781 ttgacgatga atagccaagt gtctcatcat aaacggctgt taagcgtaga aaatcttcaa
     3841 ttagggtatg aaaatctgtt attcgagcca gttcatttta cgatcgaacc tcatcagcgg
     3901 atggcaattt caggtcctaa cggcgcggga aagtcatcca ttatccatta tcttctgggg
     3961 acattcaacg gcaaggttgt aggagaaaaa tcccagccca aacatttgac cattagttac
     4021 gtacgccaaa attatgaaga caatcgaggg acattggcgg aattcgcgaa gaaaaaccga
     4081 gtagactatc aagcattttt gaataatctc cgaaagcttg ggatggaaag agatgttttt
     4141 cataataaaa tcgaacagat gagtatggga caacggaaaa aagtggaatt ggctaaatct
     4201 ttatcccaac cagctgagct ctatatatgg gatgaaccat tgaattattt ggatgtcttc
     4261 aatcaagaac aattagaaca actgattttg aacgtgaaac ctgctatgtt actagtggaa
     4321 catgatcaaa cctttctaga taaagtatct actgagatta tttctcttga gaaaatctaa
     4381 accttgtaat ctcaaagaca acaaggtatg gttaaggaga actttgtcaa cgaaggagag
     4441 aatcacatgc tttatgtaac gagacacgga gaaacaactt ggaacgcaca aggattagtt
     4501 tgcgggcgag cagatgtgcc gctgactgaa aaaggacaga tgcaggcaca ggcactagca
     4561 gaaaaggtag tagatttgcc tgttccaatc acgaaaatca tccattcccc tcttcaacga
     4621 gcaagagata cagcacaagc agtagttgat cgtctcaatt tgccgatgac cgctgatgag
     4681 cgattggtag aaatggattt tggggattat gacggactgc caagcaaaga tgaaaacttt
     4741 caaaaagcgc gtttagcgtt tgctgtacgt ttcccaaatg gagagtcggt cttagatgtg
     4801 tacgcacgga tcgttccttt attgaaagaa tgcatggaag acgaagaaaa tgtctacttg
     4861 ttagtctgtc ataatgcctt gataagagtg atcaatgctt attttcatcc aatgcctaat
     4921 gaagggttct ttacattcat ggtagacaat acagaattaa tttcttatga gtaattagac
     4981 ttttgtgtta tttaaataaa atgtttcaag gcactgcttt tgcttagcag tgcctttttt
     5041 gtggctgtgt
//
LOCUS       DACDIP010000271         4178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.guided.124, whole genome shotgun sequence.
ACCESSION   DACDIP010000271 DACDIP010000000
VERSION     DACDIP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.guided.124"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            129..1607
                     /gene="msr"
                     /locus_tag="IV257_003010"
     CDS             129..1607
                     /gene="msr"
                     /locus_tag="IV257_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8816039.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1654..2100
                     /locus_tag="IV257_003011"
     CDS             1654..2100
                     /locus_tag="IV257_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8816040.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2299..2814)
                     /locus_tag="IV257_003012"
     CDS             complement(2299..2814)
                     /locus_tag="IV257_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8816041.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2931..4132
                     /locus_tag="IV257_003013"
                     /pseudo
     CDS             2931..4132
                     /locus_tag="IV257_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1241 a          708 c          908 g         1321 t
ORIGIN      
        1 gtgatttagt catagttatt tcgttcagag taggaaaaga tgtgatgcag tttttttgca
       61 aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga aataggagtg
      121 taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc tttggcaacc
      181 agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata gggatcatag
      241 gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa ctttttcccg
      301 atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta acctatctcg
      361 ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga ctggaagaaa
      421 cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata cgtcttcttt
      481 cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat agaaaaagta
      541 ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt gttagtcatg
      601 atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac ggagaaatcc
      661 gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag aaaaagactc
      721 agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa tcgatccaag
      781 aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa
      841 tcagaccgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa
      901 agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc aagccgcagc
      961 agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc cgctatccca
     1021 tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta aatggtgatt
     1081 ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa
     1141 ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg aaagtaagct
     1201 ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc attcgttttg
     1261 tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat cacttagggt
     1321 ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa gcgacccgtc
     1381 tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat gaaccgacta
     1441 attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata tatccgggaa
     1501 cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat gaagtttatg
     1561 aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg tgctacgatg
     1621 attttcgtga caaatttaaa ggagtggtac agattgccta caaataaaaa agaaggaata
     1681 atttttacaa caatgatgtg ttttttgatg gtcttgggga tgagcgcgta taatttatgg
     1741 ctgcatcaag atcttcattt atcaagtttg ttgattggtc tagtgcctgg attcgtggtg
     1801 gctttcattt tggatgtctt catcatcggt gtgctggcga aaaaaattgc ctttaaacta
     1861 ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta tttcatgctt gatggtttta
     1921 ggaatggtga catgtatgtc tttattcggt gtgatcatgg aacaaggaat acctgatcaa
     1981 ttattctctg cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt accgttgcag
     2041 ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag caattcaaag taatgaatga
     2101 tggaacactg ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc tcttgaatgc
     2161 agttctagaa aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg atcgcttttt
     2221 cttttccaac tgtattgctc ttttattgaa gataatttgc tcttcaatag atatttctag
     2281 aaaggttctg tttatccctt actcaaaaaa ctgctgcatt tgttctctta cttgctgaca
     2341 ccattggagt tcctgctgac aaagcataat tctgcgctgg atcacaacat aacgtccaaa
     2401 ttcttgtttc ttgtctagcg agaggtcatt caattttttt tgattttttt gcatgatttt
     2461 gttcagctgt tgttctctag tgaatagcag tccagtagct gttgttttat cgagcgtcga
     2521 aaaagcatag atttttgcta gaaattcatc tttttgcgta ggagcgggag tttcctcttt
     2581 gatccattcc tctacaagag cacgtccttc atcagtcaag tggtagatct ttttttgaga
     2641 gtgctcgtct ttgtccaaaa cggtcacata accctgctgg tctaacttgg caagcgttgt
     2701 atagatctga ctgtgatggg cttcccaaaa aattgtgatc agctgcttca attcataccc
     2761 agaacgtggt ttggataaaa gcacgcataa taatatatag gctaaagtat tcataaaaat
     2821 cctacctctc gtcttatttt ctttattata tcacagtaaa gtttccacgt tttacatgtg
     2881 catatttaca tgtaaaataa atcatgtatg atgctaatag agagaaaggg atggaaatat
     2941 tggagaatca aatagatata caagaacaaa caaatgaaga aacaaagggg aaaagttcag
     3001 cgctagttgt agcagctacc tgtatgatcg cttttatggg agtgggtcta gtagacccaa
     3061 tcttaaaaac gattgctgca cagttaaacg ctacgccagc tgaaacgacc ctgttattta
     3121 ctagttacat gttagtcact ggtgtggtga tgctatttac aggtttctta tctagtcgga
     3181 tcggtttgaa aaaacattga tgacaggatt gtttatcatc gttgtattcg ctggtcttgg
     3241 cggattatct gggaatatcg gaatgctgat tgggttacgt gccggctggg gtgtcgggaa
     3301 tgctttattt gtctctactg ctttagcaac gatcgtcagt attttagttg gtaacacaga
     3361 aaaagcaatc atgatgtatg aagcagcact tggctgcgga atggctgttg gccctctagt
     3421 cggtggggtc ttgggcagtg gttcatggcg ttatccattt tttggggtag catttttgat
     3481 gttttgcggt tttatagccc tagcggtact tttacctgaa actgagaaac caaagagaaa
     3541 agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt cagttggact
     3601 aatcgcattg ttatataatt tcggattctt tactttactt gcgtatgcgc catttttatt
     3661 gattggctat tctgaattag aagtcggttt tgtatttttt ggctggggtg tgcttctggc
     3721 tattgcttcg attttcgttg caccggctct tgagcggaag tttacgactt atcggacaat
     3781 tttagcagca tttgtcttat tcattatttg tttgctgtta ttaggagctg gaacttctat
     3841 gccagtcctt gtaccgatca gcattgtgtt agcaggattt ttccaaggaa ttatcaatac
     3901 actgttgacg acgattgcta tggaaatacc aggtctgcaa cgaaacgttg cttcgtccag
     3961 ctatagtttt gtccgctttt tcggtggagc tttagcacca tttattgcag gtgtgattgg
     4021 tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt gccattgttc ttatcagtgt
     4081 atgttgtatc atttatcacc gtaattattt tgctacaaat gaagaagaat aagaaatcag
     4141 aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DACDIP010000272         2670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.guided.145, whole genome shotgun sequence.
ACCESSION   DACDIP010000272 DACDIP010000000
VERSION     DACDIP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.guided.145"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..208
                     /locus_tag="IV257_003014"
     CDS             <1..208
                     /locus_tag="IV257_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8816042.1"
                     /translation="LAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRK
                     EHGTDIDAACGQLRSKQMKKTVAK"
     gene            complement(401..1504)
                     /locus_tag="IV257_003015"
     CDS             complement(401..1504)
                     /locus_tag="IV257_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8816043.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            2057..2605
                     /gene="aac(6')"
                     /locus_tag="IV257_003016"
                     /pseudo
     CDS             2057..2605
                     /gene="aac(6')"
                     /locus_tag="IV257_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
BASE COUNT          869 a          465 c          516 g          820 t
ORIGIN      
        1 attagcatat gtcaacttga ttccatataa tccagtcagt gaacatgatc aatattctcg
       61 cagtccaaaa gctgatgtat tgagatttta tgatgtattg aagaaaaacg gcgtaaactg
      121 tgtgatacgt aaagaacatg gtacagatat tgatgcagct tgcggacaat tgcgaagcaa
      181 gcaaatgaaa aaaacagttg ccaaataatc gtatcctact cgttttttca attaccataa
      241 aaggagcagt tttcttaatt aaggaactgt tcaacttaaa taagagaaaa gattctatag
      301 cgaaagctat aaagcttttt ttgacaagaa aaaccctcat caagcaaaga aatgtcctca
      361 atttgatgac agcctgcttg atgggggttt tagcaattat ttaactgatt ttcttatatc
      421 cttgctggct ttcttgtgta gcatgataag tttctacgtc aagaatggcc tgtttaaatt
      481 tttctcgggt gatttcataa ggcacatgtt taatatcatt tgttgccatg gctttatcta
      541 agattgcttc taaatcggtc gtgacattga gattcatgtc ggttaacgat actggaagtt
      601 ggttcttttt cataaacaca tagattttat caaacaaatc gaactgctga tccatcatca
      661 ataaggtcag tacaccataa ctgacagatg ctccgtgaag gtatccctcc attttttcta
      721 gaacagtcgt accgtaatag aatgagtgtg ccatagcaga attatagtta ttgttaacta
      781 aaacagaaac caatccggta gtaacaatga tatccgaaac gacttgttca attgcatatg
      841 agggacgatc gtcggcacta tctttcaatg cttgcgtacc ataagtcaag agacgatcaa
      901 tgcacatttt tccgatttgg attcctaatc tatccgtgta gcctagatca tcgccacgag
      961 cagaaaaagt agattcgact tctttggata aagcatcgcc aatacctgcc cataagtatt
     1021 tatcaggtgc ttcggcaata attgctgtat caatgaaggt gtgtacaggt ggttttgaag
     1081 gaaagaagag tccgtccatt acgccttctt cgttatagat gacacatacc gcagtgacgg
     1141 gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg tttagctagt ttttcaccaa
     1201 tgacttttac agtatcgcaa gcacggccac caccgactgc aaacaggacg tctgcttttt
     1261 ggacactttc atgctggatc aactgttcaa tatttccata agttgaatta cccccgtacc
     1321 agagactggc gacaacctct atttcctgtg tcaaagctac ttttattttg ttttctgcct
     1381 ttgaaagtgc agtcttccct ccaatcagca ctacttttgt cccatattct ttgacgattc
     1441 catttgcttt ttcatacgca tctgttccaa tggtataatt tggtaagaaa acactttgac
     1501 tcatattcat ttccttcttt cttcgttgaa acgggttaaa attttaaaga gtgatcgatc
     1561 tgactgcggc tgtagatgga gtagaggtga acctaagaca ggataagtga tgaataaggt
     1621 ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa cgaacaagta caaaaacatt
     1681 gaaaacgaca ataatgaatt tttgttcctc ttagtatttc taccgattcc ctttgctttt
     1741 ttgtaattat ctaaaaattc taaaaattaa gattattata tgaaaacagg ttttatttgt
     1801 caattcataa ttggtttttt ttaaagcaaa agataatcta tatcatgatg aacaaacaga
     1861 gttttttcct ccaattgatg gctttcctga tctttcctat tttgaataaa gtgtttctgt
     1921 ataatttttt tattttataa gtaagataaa taatgaaaga atagagatac tgattggtag
     1981 ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag gactgttaga ataagcaaga
     2041 acaaggggga agacgtatga taatcagtga atttgaccgt aataatccag tattgaaaga
     2101 tcagctttct gatttactga gactgacttg gccggaagaa tatggagaca gctaggcaga
     2161 agaagtagaa gaaatgatga atccagaacg aatcgcggta gcagcggtag accaagatga
     2221 gttagtagga tttattggtg caatccctca atacggtatc acaggttggg aattgcatcc
     2281 attagttgta gaaagctccc gacgaaagaa ccaaataggt actcgattag tcaattactt
     2341 agaaaaagaa gtagcttcca gaggaggaat cacgatttat ttaggtacgg atgatttaga
     2401 ccatggaaca acgttaagtc aaacggacct gtatgagcat acatttgata aagtggcttc
     2461 catccagaac cttcgtgaac atccgtatga attctatgaa aaattaggtt ataaaatcgt
     2521 aggtgtctta ccaaatgcaa atggctggga caaaccggat atttggatgg caaaaacgat
     2581 tattcctcga ccaaattctc aataataaaa agaggttgtg acagaagtaa tcagcttcaa
     2641 gaaataagaa ggaatttacg aaaattactt
//
LOCUS       DACDIP010000273         1000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63715_2016
            SAMN12640301-rid9885213.guided.213, whole genome shotgun sequence.
ACCESSION   DACDIP010000273 DACDIP010000000
VERSION     DACDIP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640301
            Sequence Read Archive: SRR10040950
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742894.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:11:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,017
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 42 of 70
            Pseudo Genes (incomplete)         :: 23 of 70
            Pseudo Genes (internal stop)      :: 20 of 70
            Pseudo Genes (multiple problems)  :: 11 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640301-rid9885213.guided.213"
                     /isolate="E63715_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1000
                     /gene="tet(S)"
                     /locus_tag="IV257_003017"
     CDS             <1..>1000
                     /gene="tet(S)"
                     /locus_tag="IV257_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAP8816044.1"
                     /translation="LRQIDKAEPGEIIILKNELLKLNNVLGDKKRLPHREILENPLPM
                     LQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIVLSFLGEVQMEVTCTLI
                     QEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASIGLSVTPLPLGSGIQYE
                     SLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKYGLYYSPVSTPADFRML
                     APIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKYCANILNTKLKGNEVIL
                     IGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAFQPRRPNNRIDKVRHM"
BASE COUNT          342 a          164 c          184 g          310 t
ORIGIN      
        1 ttacgccaga tagataaggc agagcctggt gagattatta ttttaaaaaa tgagctttta
       61 aaactaaata acgtacttgg agataaaaaa agattaccac atagagaaat tcttgagaat
      121 cctcttccta tgttacaaac aacaattgaa ccatgtaaat cagtacaaag agaaaagtta
      181 ctagatgcac tttttgaaat atccgatagt gatccccttc tacaatatta tgtagataca
      241 gtaactcacg aaattgtgct atctttttta ggtgaggtcc aaatggaggt aacttgtact
      301 ctgattcaag aaaaatatca tattgagata gaaacaagaa aaccaactgt catttatatg
      361 gaaagaccat taaaaaaatc tgaatttacc attgatatcg aagtacctcc aaatcctttc
      421 tgggcttcta ttggtttatc tgtaacacca cttcctttgg gtagtggcat tcagtatgag
      481 agcctggttt ctctaggtta tttaaatcaa tcatttcaaa atgcagttat ggaaggtata
      541 cgctatgggt gtgaacaagg attgtacggt tggaaattaa cagactgtaa gatctgtttt
      601 aagtatggtc tatattacag ccctgtcagt acgccagcag atttccgaat gcttgcgcct
      661 attgtactag agcaggcttt tagaaagagt ggtacagagt tattagagcc atatcttagc
      721 ttcgaaattt atgtaccaca agaatatctt tcgagagcat ataatgatgc ttccaaatat
      781 tgtgcaaata ttttaaatac taagttaaaa ggtaacgagg tcattctcat tggtgaaatt
      841 ccagcccgtt gtattcaaga gtatcgaaac agtttaactt tctttacaaa tggacgcagt
      901 gtctgtttaa cagagttaaa aggttatcag gttactaaca ttaagtctgc tttccaacca
      961 cgtcgtccaa ataatagaat agacaaagta aggcatatgt
//
